LOCUS       DBNHXU010000001         1798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3770603, whole genome shotgun sequence.
ACCESSION   DBNHXU010000001 DBNHXU010000000
VERSION     DBNHXU010000001.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1798
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3770603"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            139..1179
                     /locus_tag="AC161X_00005"
     CDS             139..1179
                     /locus_tag="AC161X_00005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011558488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase family
                     protein"
                     /protein_id="HIR4987959.1"
                     /translation="MKAIVYHGPGLKAWEEVPKPIIIDSTDVIVRVDAVTICGTDLHI
                     LKGDVPAVTDGRILGHEAVGTVEEVGSGVKTVKAGDRVLVSCITACGSCRYCRAGQYG
                     QCLGGGGWILGHKINGTQAEYVRVPFADTSTYPVPAGVSDEQILMMADILPTGYEVGV
                     LNGRVGPGDVVAVVGAGPIGLAAILGARLYSPSHIIAIDLADSRLEAAKQFGADLTIN
                     NGREDAVRIVKEMTDGLGADVSMEAVGVPAAFELATSLVRPGGRIANIGVHGEPATLH
                     LEELWIKNVTITTGLVDTYSTPTLLGLIQSGMINPSPLVTHTFKMEQFEEAYDVFGRA
                     SETGALKVVLAR"
     gene            1220..1612
                     /locus_tag="AC161X_00010"
     CDS             1220..1612
                     /locus_tag="AC161X_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2237 family protein"
                     /protein_id="HIR4987960.1"
                     /translation="MSERNVLGGDLEPCGVDPLTGFYRDGTCVTGPEDIGSHTVCAVV
                     TSEFLDHQRGIGNDLSTPMPEYLFPGLVPGDRWCVTASNWLRAHHDGAAAPVVLASTH
                     ERALDLISLETLREHSVDVPADPGILEG"
     gene            1623..>1798
                     /locus_tag="AC161X_00015"
     CDS             1623..>1798
                     /locus_tag="AC161X_00015"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4987961.1"
                     /translation="MLPAVDVSGLSVSYQKTGHGPPLVLLHGFAVDSRVWRPQLESLS
                     DQFTVIAWDAPGAG"
BASE COUNT          321 a          557 c          575 g          345 t
ORIGIN      
        1 gacggcggtg gctgggacat cttcgcctgc cggggctgag cagccgcatt acggccgttt
       61 ggccctacaa gtcttcaccc ctccggccgt acggtcgcgg tagttggaac tttcacgccc
      121 ctcgagagga ggcccctcat gaaagcaatc gtctaccacg gcccaggcct caaggcttgg
      181 gaggaagtcc caaagccaat catcattgac agcacagacg tcatcgtccg cgttgacgct
      241 gtgaccattt gcggaacgga cctgcacatt ctcaaaggtg atgtccctgc ggttaccgat
      301 ggccggatcc tcggccacga ggccgtcgga accgtcgaag aagtgggctc cggagtcaag
      361 accgtcaaag caggagatcg ggttctggtc tcgtgcatca ccgcgtgcgg atcctgtcga
      421 tattgccggg cgggtcagta tggccagtgc cttggcggcg gcggatggat ccttggccac
      481 aagatcaatg gcacccaggc cgaatacgtc cgggtcccgt tcgccgacac atcgacctac
      541 ccggttcccg ccggcgtcag cgacgagcag atcctgatga tggctgacat cctcccgacg
      601 ggttacgagg ttggtgtcct caatggcagg gtcggtccgg gcgatgtcgt cgcggtggtc
      661 ggtgcaggcc caatcggcct cgctgccatc cttggtgcca ggctctacag ccctagccac
      721 atcattgcga tcgatctggc ggacagcagg ctcgaggctg cgaagcagtt cggagctgat
      781 ctcacgatca acaacgggcg cgaggacgcg gtgaggatcg tcaaggagat gaccgacggc
      841 ctcggtgccg acgtgtcgat ggaagccgtc ggagtccccg ccgcgttcga gttggctacc
      901 tcgttggtcc gcccgggagg caggatcgcc aacatcggag ttcacgggga acctgcgacc
      961 ttgcatctcg aggagctgtg gatcaagaac gtcaccatca cgacggggct ggtggacacc
     1021 tactccaccc cgacactgtt gggcctgatc cagagcggga tgatcaaccc ttccccgctg
     1081 gtcacccaca ccttcaagat ggagcagttc gaagaggcat atgacgtgtt cggtcgggca
     1141 agtgagaccg gtgctctgaa agtggtgttg gccagataac tggccacttg cgtttcacca
     1201 gaacgcgata ctggggccgg tgagcgaacg caacgtgctg ggcggtgact tggagccctg
     1261 tggcgtggat ccgctcaccg gcttctatcg cgacggcacc tgcgtgaccg ggccggagga
     1321 cattggtagc cacaccgtgt gcgccgtggt cacttccgag tttctcgatc atcaacgcgg
     1381 gatcggcaac gacttgagca cgccgatgcc cgagtatctg ttcccgggcc tggtgcccgg
     1441 cgaccgttgg tgtgtgacag cttcgaattg gctccgggcg catcacgatg gggcggcggc
     1501 gccggtcgtt ctcgcttcca ctcacgagcg ggccctagac ctgatatcgc tggagacgct
     1561 tcgggagcac tcggtcgacg tgcccgctga tcccggcatc ctcgagggct gattcggcca
     1621 gcatgttgcc agcggttgac gtgagcggcc tttccgtcag ttaccaaaag accggccacg
     1681 gcccacccct tgttctcttg cacggctttg cggtcgactc ccgggtctgg aggccgcaac
     1741 tcgaaagcct gtcggaccag ttcactgtca tcgcatggga cgctcccggc gccggtca
//
LOCUS       DBNHXU010000002         2281 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4762923, whole genome shotgun sequence.
ACCESSION   DBNHXU010000002 DBNHXU010000000
VERSION     DBNHXU010000002.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4762923"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /gene="lipA"
                     /locus_tag="AC161X_00020"
     CDS             <1..769
                     /gene="lipA"
                     /locus_tag="AC161X_00020"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HIR4987962.1"
                     /translation="NCPNIYECWGMGTATLMILGDKCTRACSFCNVNTGKPTEFDIFE
                     PYRAAEAIRKMGLKHAVITSVNRDDLDDGGSGIFAHTITETRRQSPGTEIEVLIPDFK
                     GDREALQTVMDAVPEVLNHNTETVLRLQRDIRTSANYGRSLALLWRAKQMNPSGLVKS
                     GLIVGMGETREEVLDTLADMRAVGIDIITIGQYLRPTARHRSIHRYVHPDEFAEYKSF
                     GEGRGIPHVESGPLVRSSYHAKESRAAVPVTIGSSAG"
     gene            complement(841..>2281)
                     /locus_tag="AC161X_00025"
     CDS             complement(841..>2281)
                     /locus_tag="AC161X_00025"
                     /inference="COORDINATES: protein motif:HMM:NF045027.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4987963.1"
                     /translation="RNRVLGLRSLWVTAMRLRIVITLIIAFVMAQGVYPAAADPVAVR
                     DFEDDTWTDGLVDVRTTDLSRTDVVTSGFAGNGLTVIIPEGAFRGLGPFDRLPLPVPN
                     EAWYRYHIRLTSWNSADTGKLPGLSGSYSSSAKGCIPSTPDEPGWSARGLFGVPGTNG
                     APAGHVPIGTYLYHLDQVGACGDELFWPGADLEPGRWQCIEGHVELNTPGANDGSVEG
                     WLDGTKRFTRTGLAFRRTGEPAIGIREMWLNVYFGGSWPTPNPLSLTIDQVVVSTEGR
                     VGCLDPFTDDNNSLHKKAFTELHARGLLYGCGYRLACPQRLMTRGEVAALFARVLGLP
                     PATKNYFNDDDGHLFEGAINKVAAAGITVGCGAGAFCPDRDLTRAEFATMTVRAMGLS
                     PAGGDAFTDDDGHWAESAINTFADAKLTEGCGSNRFCPNRVLSREEAASFFLRVADRL
                     EPLHPASVEPPPSWPPPGDPPPIPPGERD"
BASE COUNT          434 a          694 c          751 g          402 t
ORIGIN      
        1 caactgcccc aacatctacg aatgctgggg gatgggcacc gccacattga tgatcctcgg
       61 cgacaagtgc acccgggctt gctcgttctg caacgtcaat acggggaagc cgaccgagtt
      121 cgacatcttc gagccgtatc gggccgcaga agcgatccgg aagatgggtc tcaaacatgc
      181 cgtgatcacc tcggtcaacc gcgatgacct ggacgacggt gggtcgggca tctttgccca
      241 caccatcacc gagacccggc gccagtcgcc aggcaccgag atagaagtac tgatcccaga
      301 cttcaagggt gatcgcgagg cgctgcaaac tgtgatggat gcggtcccag aggtgctcaa
      361 tcacaacacc gagaccgtgc tccggttgca acgcgacatc cggacttcgg ccaactatgg
      421 gcgctcgctg gcgcttctat ggcgcgcgaa gcagatgaac ccgtccggcc tggtcaagtc
      481 gggcctgatc gtggggatgg gggagactcg cgaggaagtg ctcgacaccc tggccgacat
      541 gagggcggtg ggcatcgaca tcatcacaat cggccaatac ctgcgcccca ccgcaaggca
      601 tcggtcgatc catcgctacg tccaccctga cgaattcgcc gagtacaaga gctttggcga
      661 ggggaggggc atcccgcatg tggagtcggg tcccttggtg cgttccagct atcacgccaa
      721 ggagagccgg gcggccgttc cggtgacgat cgggtccagc gcgggctagg cccgcttcgt
      781 aattgcgttc gggaggtcgt atgtgatccc tgtcgcacac aattaggcga ggggggccgc
      841 ctagtcgcgt tctcccggtg gaatcggtgg cgggtcgccg ggcggcggcc aggagggcgg
      901 cggttcgacc gaggccgggt gcaacggctc gaggcggtct gccacccgaa ggaagaagct
      961 tgctgcctcc tctcgagaca gcacccgatt aggacagaag cggttggagc cgcagccttc
     1021 ggtgagtttg gcgtcggcaa aggtgttgat ggccgattcg gcccagtgcc catcgtcatc
     1081 agtaaaggcg tcgccaccgg cgggggatag acccatggct cgcaccgtca tggtcgcgaa
     1141 ctcggctcgg gtcaggtcgc ggtcggggca gaacgctccg gctccacagc caactgtgat
     1201 cccggcggcc gccactttgt ttatcgcgcc ttcaaacaga tgcccgtcgt catcgttgaa
     1261 gtagttcttg gtggctggcg gaagacccag cacccgggca aacaaggcgg ccacctcccc
     1321 tctcgtcatc aagcgctgcg gacaggcaag ccgatatcca catccgtaga gcagcccgcg
     1381 ggcatggagc tcggtgaagg ccttcttgtg aaggctgttg ttgtcgtccg tgaatgggtc
     1441 gaggcatcca actcttccct ccgtcgagac cacgacctgg tcgatggtca gactcaacgg
     1501 gtttggagta ggccagctgc ccccgaagta gacgttgagc cacatctcgc ggattccgat
     1561 ggcaggctct ccggtgcgtc gaaaggcaag accggtgcgg gtgaagcgct tggtcccgtc
     1621 cagccatccc tcaaccgacc catcgttggc gcccggggta ttcaactcga cgtgtccttc
     1681 gatgcactgc caacgaccgg gttctagatc ggctccgggc cagaagagct catcgccaca
     1741 tgcgccgacc tggtctaggt gatagaggta ggtgccgatg gggacgtggc cggccggagc
     1801 gccattagtg ccggggacac cgaacaggcc gcgggcggac cagcccggtt cgtcgggcgt
     1861 tgaaggaatg catcccttgg ccgacgacga gtagctcccg gacaggccgg gcagtttccc
     1921 ggtgtcggcg gaattccaac tggtgagccg tatgtgatat cggtaccagg cctcattggg
     1981 aaccggcagg gggaggcgat cgaatggccc caggccacgg aacgctccct ccgggatgat
     2041 gacggtgagg ccgttcccgg cgaatccgga ggtgacgaca tcggttctgg agaggtccgt
     2101 cgtccggacg tcgaccagcc cgtccgtcca tgtgtcgtct tcgaagtcac gtaccgcgac
     2161 cggatcggct gcggccggat acacgccttg agccatgacg aaggcgatga tgagggtgat
     2221 gacgattctc aggcgcattg ccgtcaccca aagagaccgt agcccgagaa cccgattacg
     2281 c
//
LOCUS       DBNHXU010000003        12645 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_794415, whole genome shotgun sequence.
ACCESSION   DBNHXU010000003 DBNHXU010000000
VERSION     DBNHXU010000003.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 12645)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12645)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12645
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_794415"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="AC161X_00030"
     CDS             complement(<1..513)
                     /locus_tag="AC161X_00030"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4987964.1"
                     /translation="MKVLAIAWNNVRRMLRERSNIFFVFIFPLALILLIGAQFGGGVD
                     PVVGLYLADDGRLAAAVADAVEAEETLDVIRFENEEALATAVEHGGAQAGLFLPAGMD
                     ETAEAGEQVEMGFVTRPDGFGPQIQSVVGAAISEVMAPVTAAQFAMDKAGASFDEALT
                     TAQEQVSAAPG"
     gene            complement(510..1691)
                     /locus_tag="AC161X_00035"
     CDS             complement(510..1691)
                     /locus_tag="AC161X_00035"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4987965.1"
                     /translation="MGPATRIATKDLKLRIRDRSAFILGIFAPLILAIIFKFVFGAAF
                     TADSLDLRFGFVDLDGSETSQAFHDVLEGAESEDILTLTEFDDADGADVALEAGDINA
                     YYLIDPGLEEAVRAGDDFVIRVVGDVDAQTSTQIAASFAEQFGAGVAGVQLAVATSVH
                     LLDGPPPQDVSTWGQEAAGRGPSFALTEVATEVRQLDGTTYFAAGMAVFFLFFTVQYG
                     VIGLLEEQRDGTLARLLAAPIRRTSVISAKALLSYWLGVISLGVLIVATKFLMGANWG
                     PTLGVILLVLAGVFAAVAIMGLVGSVAKSPEGAGNLASIVAVILGMLGGTFFPVGQGD
                     DFLSKLTYLTPHAWFLRGLANISGGASWTAVLPAVAALLIFGLVTGSIAWVALRRKIT
                     V"
     gene            complement(1694..2605)
                     /locus_tag="AC161X_00040"
     CDS             complement(1694..2605)
                     /locus_tag="AC161X_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4987966.1"
                     /translation="MVKKFGDLTAVAGVGFSIEEGETFGLLGPNGAGKTTSISMIAGL
                     LEPDAGEIMVAGTAISPTSIAGKASIGLVPQDLAIYPDLTASENLAFFGRLYGMKGDE
                     LERRVDEVLEVIALNERKDDLTKEFSGGMKRRLNIGIGLLHRPKLLILDEPTVGVDPQ
                     SRNSILESVESLSAEGMAVLYTTHYMEEAERLCDRVAIIDEGTIKAEGTRRELVSMVG
                     ERDRVAIAATGDMTTAIQAALGIPGVFEASQSDQGLDVLADEASGLLPELLGKIASAG
                     AVITGVDVIEPNLEAVFLHVTGKALRD"
     gene            complement(2651..4054)
                     /locus_tag="AC161X_00045"
     CDS             complement(2651..4054)
                     /locus_tag="AC161X_00045"
                     /inference="COORDINATES: protein motif:HMM:NF015983.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HIR4987967.1"
                     /translation="MRLLLVSTYELGHQPVHLALPAAELSATGVEVELLDLSIEPFDV
                     AVLQGVDAVAFSVPMHTAMRLAIEVASMVRAARPELPIAFYGLYAAVGRDGTVGRVAD
                     LLISGEYLPGLKAWADALANGREPLQGVVLGLDRPTAVVPDRTTLPSNDGYARLEWEG
                     EARVAAAVEASHGCRHRCRHCPIPVVYDGRIRLAGLEPVLADIANLVADGVEHVTFAD
                     PDFFNAPRYSTDILRAAHDAYPDLTFDVTVKVEHILAHDSVWTELSGLGLLFVVSAFE
                     SVDERTLQILDKSHTVEDMSGAVRILRGSGVYVRPTWLPFLPWTDPDHLVGMVEFIDS
                     HQLWSAMDPVQMAIKLLIPEGSLLEGHPAVTPFLRSYDPMTLTWAWEFEHAASELMHK
                     ELDAIAADGSDCGAESVETLGLMRLAIARGAGVDLGPMPRLGRPTPRLTESWFCCAEP
                     TAGQAIAAGLSIGRVPG"
     gene            4096..4980
                     /locus_tag="AC161X_00050"
     CDS             4096..4980
                     /locus_tag="AC161X_00050"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4987968.1"
                     /translation="MGEYVTVSTGQVFRENFGGSGDLILLVHGLGGSTANWLAVTAGL
                     ARHGRVVGLDLPGFGVSPPARDYRLETHRQAIEDLVEIEGGRATLIGNSTGGLLSQMV
                     ASHRPDLVDKLVLVAPASPPVFPDPRLDWPTAIRLALQATPGIGITYGRRFVDGHTPE
                     ELVRLSMQMITHSMGRVPMEVIEASVSMARIRKELPWAAEATARTATSIAACYANRAR
                     YIRMIRAIVAPTLVVQGLSDRIVSPTAVERICELRPDWNLVQLRDTGHTPQMDAPLRF
                     LAVVEPWLSEVESRAVGA"
     gene            5023..7740
                     /gene="aceE"
                     /locus_tag="AC161X_00055"
     CDS             5023..7740
                     /gene="aceE"
                     /locus_tag="AC161X_00055"
                     /EC_number="1.2.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring),
                     homodimeric type"
                     /protein_id="HIR4987969.1"
                     /translation="MPGDHGLILGDEFIHQLPDTDPTETQEWVDSFDAVVHEQGPDRA
                     RFLMAKLLERAHETSIGVPGSVTTPYVNTISAEDQPEFPGDEDIERRIRRFIRWNAAV
                     MVIKANHREKGIGGHLSTFASSATLYEVGFNHFFQGRNAPGGGDHVYMQGHAAPGIYA
                     RAFLEGRFDEKHLDNFRFEIGGDGLSSYPHPRLMPDFWEYPTVSMGIGPINSIYHARF
                     NKYLENRGLDDTSQSRIWCFIGDGETDEPETLGAISLATREGLDNLTWVINCNLQRLD
                     GPVRGNGQIITELEAVFRGAGWNVIKVIWGTGWDRLLANDQDGALIGVMSSTVDGEYQ
                     RYKAEDGAYVRDHFFGKDPRTKAMVDDMTDDQIWDLRRGGLDHRKVYAAYKVASELKN
                     GKPTVILAKTIKGWTLGPDVEGRNATHQIKTLTNQQLMDLRETLHLDKEIPAESLEGE
                     SEPPYFRPGPGTPEYEYLQQRRQELGGLLPSRVVAESSLQLPAPEAYTEIHAGSGKVE
                     ASTTTAFTRLIRNLSRVEGFGDRIVPIVPDEARTFGMDSLFRELKIYASRGQLYESVD
                     ASMLLSYTEAQDGQILEEGITEAGSVASWIAAATSYATRGLPMIPFFIFYSMFGFQRV
                     GDLIWAAADSRARGFLLGATAGRTTLQGEGLQHQDGHSLVLATTVPVCEAYDPAFAYE
                     VGTIIEHGLQRMYGAGEDRFYYLTLYNENYAQPPRPDGVSDGIIEGLYRWQVAPGDNG
                     PRTAILFSGSANLAAREAAEELAQNYGVRADLWSATSYKKLREEALGVDRWNRLHPGE
                     TPRVPLVSRRLADVGGPVIAVTDFMKLVPDQVAQWVPQRFVTLGTDGFGRSDSRQALR
                     KFFEIDAGSIVAATLWALANDDLVPRSAVLDAFTRYGIDPEAPDPAHQH"
     gene            7767..8153
                     /locus_tag="AC161X_00060"
     CDS             7767..8153
                     /locus_tag="AC161X_00060"
                     /inference="COORDINATES: protein motif:HMM:NF025819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008235 - metalloexopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HIR4987970.1"
                     /translation="MPEPIRQAILTHAENSLPDECCGLIASVEGGIRFAYPLTNSNPS
                     PVSFTVDPGEHFGAMSHAENQGWEISGVFHSHPTGEPSPSATDVAMAWDPLWIHLVVA
                     RGEIAAFRIVDGEVEAVELWLVAKGP"
     gene            8204..8689
                     /locus_tag="AC161X_00065"
     CDS             8204..8689
                     /locus_tag="AC161X_00065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter regulatory subunit"
                     /protein_id="HIR4987971.1"
                     /translation="MPRIEESALPGVGVRYEFKTDDGRRIGVVHHRTGRRELFVCEPD
                     DTDAAVVNMSLSDEESHALSEALGGSTVVANLANLQQQVEGLAIDWLSIAPGSPYAGK
                     TIADARIRTRSGVSVVAVLRSGETFPSPDPGFAIEPGDTLVVVGTPDGIKQTQEILRT
                     G"
     gene            8693..9850
                     /locus_tag="AC161X_00070"
     CDS             8693..9850
                     /locus_tag="AC161X_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014986711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR4987972.1"
                     /translation="MDESALFLIELGAVILALGVMARLAGRLGISPIPLYLLAGLALG
                     EGGVFPVITSEGFIEFGAELGVIFLLLMLGLEYSARELVQGVRSVASVGLADFLLNFT
                     PGFVAGIVLDLGLVPAVFFGGVTYVSSTGIVAKLLEDLGWSGNRETPVVLSILVFEDL
                     AMAVLLPILGVLALGSSMSSAVLTVGLAVVVIVGFIGLVSRGGDRLGRLVFHHSDEVS
                     LLSLFGLTLVVAGLAEGVNISAAVGAFLVGIGVSGDSVDRARSLLTPMRDLFAAVFFV
                     FFGLQTDPGDIPEVAVAAITLAIVTGLTKVLVGWWAASRAGVGRQGRLRAGILLVARG
                     EFSIVIAGLAVSVAGGSQLKPLAATYVLIMAIAGPIAAKVADARRKHVILV"
     gene            complement(9861..10880)
                     /locus_tag="AC161X_00075"
     CDS             complement(9861..10880)
                     /locus_tag="AC161X_00075"
                     /inference="COORDINATES: protein motif:HMM:NF019351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR4987973.1"
                     /translation="MPEGVSRRDLLKKVGLGGAVFLGGSVLGHTIQHQAQAADTEDYD
                     PGSEHAMPGESDDGKYSNLHSPPTSLGPGDLDHQTFPPPRDGQPAGVVRELELAVEDR
                     LIEVGDGVLFEAWTYNGTVPAPIIRATEGDTLRISFENRTGHPHNLHFHGAHRPEQDG
                     WEQVPPGGKTTYEIEAGPVGVHPYHCHTTPLAEHIGRGLYGMMIVDPVEERAPATEVA
                     LLVSGFSSEALGVNGVIAWNGVAGFYNSYPIKVPVGEPVRAFVVNMLEHEPIASFHLH
                     AETFDVYPAGMGGTPAFRDDTIVLGQAQRAMIEFTLPSLGRYMFHPHQHWLAERGAMG
                     WFAAV"
     gene            complement(10889..12535)
                     /locus_tag="AC161X_00080"
     CDS             complement(10889..12535)
                     /locus_tag="AC161X_00080"
                     /inference="COORDINATES: protein motif:HMM:NF024627.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="right-handed parallel beta-helix
                     repeat-containing protein"
                     /protein_id="HIR4987974.1"
                     /translation="MSRRRGLLVVLVIGLAACAGPPVGDPSPAALGDPATDSPMEWSG
                     ETRLVPQDYPTIQSAVDAADPGDLVLIDRGVYLEEVEVTTPGLTIRGVDRNDVIIDGE
                     MQRTNGVEVFFADGVAVENLTVRNATGNGVFWTGVRGFRGSYLTALNNNDYGIYAFDS
                     SDGLMEFSYAFGSPDAGFYVGQCDPCQATITDSIAEWNGLGFSGTNASNDTYIVNSVW
                     RYNLSGIAPNTLDSELLPPSRGVTVVGNLVYDNGNVKAPAKALQWAAQGNGIMLAGGG
                     DAYVARNRVFNHSQSGIVILPMLDANMYMSFDHHVEGNVVEGSGLADLTLGGPADSGN
                     CFEDNEYTSTLPAALELKQPCEGLRLPALFEFGSSSAQLGRLFESGVGLQPEFDPAAV
                     PEPERQPSMPGGADAPVIPAVNVFAGSKPDLDSITTPEMPPGLEVTQKRGINIMGVSL
                     ASAAGVFFGLYAYVLPLVLYTSWVVIAIWDLSRREDVSRGKAIGWMGAILVIPFLGVI
                     AYYLFGKSTIPVWQRWTFLAGGMAVYLFFLALGMVVGGIV"
     gene            complement(12532..12639)
                     /locus_tag="AC161X_00085"
     CDS             complement(12532..12639)
                     /locus_tag="AC161X_00085"
                     /inference="COORDINATES: protein motif:HMM:NF012355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR4987975.1"
                     /translation="MDFDQPGEYKFFCTFHGNAEGSGMAGTVIVEALEG"
BASE COUNT         2353 a         4061 c         3981 g         2250 t
ORIGIN      
        1 cccgggagcc gcagacacct gctcctgagc cgtcgtcagc gcttcgtcga aagaggcacc
       61 cgccttatcc atcgcgaact gtgcggcggt caccggtgcc atcacctcgg agatcgccgc
      121 gccgaccaca gattgaatct gtggaccgaa tccgtcgggc cgagtaacga atcccatctc
      181 gacctgctca ccggcttcag cggtctcatc catcccggca gggaggaaca gaccggcctg
      241 ggcaccacca tgctccaccg ctgtggccag cgcttcctca ttttcgaagc ggatgacgtc
      301 gagggtttcc tctgcttcga ccgcatctgc gactgcagcc gccaatcggc cgtcgtcggc
      361 gaggtagagg ccgactaccg ggtcgacgcc tccaccgaac tgggccccga tgaggaggat
      421 caaggccaag gggaagatga agacgaaaaa gatgttggag cgctcccgca gcatccttcg
      481 cacgttgttc caggcgatcg ccaagacctt catacggtga tcttccgacg aagggcgacc
      541 caggcgatgc tgccggtgac cagtccgaat atcagcaggg ccgccactgc gggcagcacg
      601 gcagtccacg aggccccgcc ggagatgttc gccaaacctc tcaggaacca ggcatgaggc
      661 gtcaggtagg tgagtttgct cagaaagtcg tcgccctggc caaccgggaa aaaggtgccc
      721 ccgagcatgc ccaggatcac tgcaactatc gacgccaggt tgcccgcacc ctcggggctc
      781 ttggccacac tccccaccag ccccatgatc gccacggcgg caaacacgcc cgcgagcact
      841 aggaggatca ctcccagagt tgggccccag ttcgcaccca tcaagaattt ggtggcgacg
      901 atgaggactc cgagggatat gaccccgagc cagtaggaga gcagggcctt ggcggagatc
      961 acacttgtgc gccggatcgg cgctgccagg agacgagcga gggttccgtc gcgctgctct
     1021 tcgaggagcc cgatcacccc gtattggacg gtgaagaaca ggaagaagac ggccatcccg
     1081 gccgcgaaat aggtggtccc atccagctga cgaacctcgg tcgccacttc ggtcaatgcg
     1141 aacgaagggc cgcgccctgc agcctcctgg ccccaggtgg aaacatcctg gggtggaggt
     1201 ccatccaaca aatgaaccga ggtcgccacc gccagttgga ccccggccac acctgccccg
     1261 aattgctcgg cgaaggaggc ggcgatctgg gtggaagttt gggcgtcgac atcgcccacg
     1321 acccggatga cgaagtcgtc tccggcgcgc acggcctcct ccaagcccgg atcgatgagg
     1381 tagtaggcgt tgatgtcgcc cgcctcgagc gcgacatcgg cgccatcggc atcgtcgaac
     1441 tcggtcaggg tgaggatgtc ctcgctctct gccccttcga ggacgtcgtg aaaagcctgt
     1501 gaggtctccg agccgtcgag gtcgacgaag ccgaagcgaa ggtcgaggga atcggcggtg
     1561 aaggcggcgc cgaacacgaa cttgaagatg atcgcaagga tcaagggggc aaagatcccg
     1621 agtatgaagg ccgaacggtc ccggatccgg agtttgaggt ccttggtggc gattcgggtg
     1681 gctgggccca cgactagtcc cgcagcgctt tgcccgtcac atggagaaaa accgcctcca
     1741 ggttgggctc gatcacatcc accccggtaa tcactgcgcc tgcagacgcg atctttccga
     1801 gcaattccgg caacagaccg gaagcttcgt cggccaatac atccaggccc tgatcggatt
     1861 ggctggcctc gaacacgccc gggatcccga gtgcagcctg gatcgctgtt gtcatgtcgc
     1921 cggtcgccgc gatggcgacc cggtcacgct cgccaaccat cgaaaccaac tcgcgtcggg
     1981 ttccctctgc cttgatggtt ccttcgtcga tgatggccac ccgatcgcag aggcgctccg
     2041 cttcctccat gtagtgggtc gtgtagagca cggccatccc ctcggccgag aggctctcca
     2101 ccgattcgag gatcgagttc cgcgactgag ggtcgacgcc aaccgtcggc tcgtcgagaa
     2161 tcaacagctt gggccgatgg aggagaccga tcccgatgtt gagcctccgc ttcatcccgc
     2221 ccgagaactc cttggtgaga tcgtccttcc gttcgttcag ggcgatcacc tccagaacct
     2281 cgtcgactcg cctctcgagc tcgtcaccct tcatcccgta gaggcgcccg aagaaggcca
     2341 ggttctcgct ggccgtgaga tcggggtaga tcgccaggtc ctggggaacc aatccgatgg
     2401 atgccttgcc ggcaatcgaa gtcggggaga tcgcagtgcc ggcaaccatg atctcgccgg
     2461 cgtccggttc gagaagaccg gcgatcatcg agatcgaagt ggtcttgccg gccccgttgg
     2521 ggccgaggag cccgaacgtc tccccctctt cgatcgaaaa accgactccg gcgaccgcgg
     2581 tgagatcgcc gaatttcttg accatgccat ttgtggtgag aacgctcgcc aagatgcctg
     2641 cctgtcgtcg ttagccaggc accctaccga tgctcaggcc ggccgcgatg gcctggccag
     2701 cagttggctc ggcgcagcag aaccagctct ctgttaaccg tggtgtcggc cgtccgaggc
     2761 ggggcatcgg cccgagatcg acccctgctc cccgggcaat ggcgagacgc atcaggccca
     2821 gagtctcgac cgactcggcg ccgcagtcgg agccatccgc ggcgatggcg tcgagctcct
     2881 tgtgcatcag ctcggaggcg gcgtgctcga actcccacgc ccaggtgagg gtcatcgggt
     2941 cgtagctccg tagaaatggc gtgacggccg ggtgcccttc gaggagcgag ccttcgggta
     3001 tcaagagctt gatggccatt tgcaccgggt ccatcgccga ccacagctgg tgggaatcga
     3061 tgaactccac cattccaacc aaatggtcgg gatccgtcca gggcaggaac ggcagccagg
     3121 tgggtcgcac atacacgccg gaaccacgca ggattcggac cgcgcccgac atatcttcca
     3181 ctgtgtggct cttgtccagg atctgaagag ttctctcgtc gacactctca aaggcactga
     3241 cgacgaagag gagcccgagc ccggacagtt cggtccacac cgagtcgtga gccaggatgt
     3301 gctccacctt gaccgtcacg tcaaaagtca ggtcggggta ggcatcgtgt gcggcacgca
     3361 ggatgtcggt gctgtatcga ggggcgttga agaaatcagg gtcggcgaag gtgacatgct
     3421 cgactccatc ggcaaccaag ttggcgatgt ctgcgaggac cggctcgaga ccggcaagcc
     3481 ggatcctccc gtcatagacg acagggatcg ggcaatgccg gcagcgatgt cgacacccat
     3541 gggatgcctc gaccgcagcc gccacccgtg cctccccttc ccattcgaga cgcgcataac
     3601 catcgttgga ggggagagtc gtccgatcgg gaacgaccgc agtcggtcgg tcaagcccca
     3661 gcaccacgcc ctgaagtggc tctctgccgt tagcgagggc gtcggcccag gccttcagcc
     3721 caggtaggta ctcgccagag attaaaaggt cggcgactct ccccactgtc ccgtcacggc
     3781 cgaccgcggc gtagaggccg tagaaagcga ttggcagctc gggccttgcc gcccgcacca
     3841 tcgaggcaac ctcgatggcc aggcgcatgg cagtgtgcat cggtaccgag aaggcgaccg
     3901 cgtcgacccc ctggagaact gcaacgtcga acggctcgat cgagaggtcg agcagttcga
     3961 cttcgactcc cgtggcagac agctcggccg cgggcagggc cagatgaacg ggttgatggc
     4021 cgagctcgta ggtggagacg aggaggaggc gcacgactct gagtctaagg acaggcggtc
     4081 ggtagccttt tcccgatggg tgagtatgtg accgtctcca ccgggcaggt gttccgggag
     4141 aacttcggtg gaagtgggga cctgatcctc ctggttcacg ggctcggcgg atcgacggcc
     4201 aattggctcg ccgtcacagc cgggctcgcc aggcatggga gggtggtcgg ccttgacctt
     4261 cccggattcg gagtcagccc accggcacgt gactaccgcc tcgagactca ccgccaggcc
     4321 atcgaggatc tcgtggagat cgagggtggc cgggccaccc tgatcggcaa ctcgaccggc
     4381 ggcctgctct ctcagatggt cgcttcccac cggcccgatc tcgtcgacaa gctcgtgctg
     4441 gtagcgcccg catccccacc ggtcttcccg gatccgagac tcgactggcc gacagcaatc
     4501 cgtcttgccc tccaagccac ccccggtatc ggaatcacct atgggcggcg gttcgtcgac
     4561 ggccacaccc ccgaggaatt agtccggctc agcatgcaga tgatcaccca ctcgatggga
     4621 cgggtgccta tggaagtcat cgaggcatcg gtttccatgg cacgaatccg caaggagctc
     4681 ccctgggcgg cggaggcgac ggcacgaact gcgacctcga tcgcagcctg ttacgccaac
     4741 cgggcccgat acatccgcat gatccgggcg atcgtcgcac ccaccctcgt ggtgcaaggt
     4801 ctctctgatc gaatcgtctc accaaccgcc gtcgagcgca tttgtgagct gaggcccgat
     4861 tggaacctcg tgcagctgcg ggacaccggt cacactcctc agatggacgc gccgttgcgg
     4921 ttcctcgcgg tagtcgagcc gtggctgagc gaagtggaga gtcgagccgt cggagcttga
     4981 cccctgggag caccgcgccc agccctaact agcattcagc aaatgccagg cgaccacgga
     5041 ttgatcctcg gagacgagtt catccaccag cttccggaca ctgatcccac agaaacccag
     5101 gagtgggttg actctttcga cgccgtcgtc cacgagcagg gcccggaccg ggcccgattc
     5161 ctcatggcca aactcctcga gcgtgcccac gagaccagca tcggcgtgcc gggatcggtg
     5221 acgaccccat atgtgaacac catctcagcc gaggatcagc cggagttccc tggcgacgag
     5281 gacatcgagc gccgcatccg ccgattcata agatggaacg cggccgtcat ggtgatcaag
     5341 gccaaccatc gggaaaaggg aattggtggc catctctcca ccttcgcctc ctcggcaacc
     5401 ttgtacgagg tcggcttcaa tcacttcttc caaggccgga acgccccagg cggtggcgac
     5461 cacgtctaca tgcagggaca cgccgctccc ggcatctatg cccgggcctt tctggaaggc
     5521 cggttcgacg agaagcacct cgacaacttc cggtttgaga tcggcggcga tggcctctcc
     5581 tcctatcccc acccccgtct catgcccgac ttttgggaat accccaccgt gtcgatgggg
     5641 atcggtccga tcaactcgat ctatcacgca aggttcaaca aataccttga gaaccgaggc
     5701 ctcgacgaca cgtcacagtc gaggatctgg tgtttcatcg gtgacgggga gaccgacgag
     5761 ccagaaacgc tgggcgcgat ctccctggcg acgcgggaag gcctcgacaa tctgacctgg
     5821 gtcatcaact gcaacctgca gcgactcgac gggccggtgc ggggaaacgg gcagataatc
     5881 accgagctgg aagccgtgtt ccgcggggcg ggatggaacg tgatcaaggt catctggggc
     5941 acgggctggg accgcctgct cgccaacgac caggacggcg cactgattgg ggtgatgagc
     6001 agcaccgttg acggggagta ccagcgctac aaggccgagg atggcgccta cgtccgcgat
     6061 cacttcttcg gcaaggaccc caggaccaag gcgatggtgg acgacatgac cgacgaccag
     6121 atctgggacc tgcgtcgcgg tgggctcgac caccgcaagg tgtacgccgc ctacaaggtg
     6181 gccagcgagc tcaagaacgg caagccgact gtgatccttg ccaagacaat caagggatgg
     6241 actctcggcc ccgacgtcga gggacgcaac gccactcatc agatcaagac tctcaccaat
     6301 caacaactga tggaccttcg ggagacactc cacctggaca aggagattcc cgccgagtcg
     6361 ctcgagggcg agagcgagcc cccgtacttc cgcccaggcc cgggcactcc tgagtacgaa
     6421 tacctgcagc agaggcgtca ggagctcgga ggcctgctcc cgagccgggt cgtggccgag
     6481 agcagcctgc agctccctgc accagaggcg tacaccgaga tccacgccgg ctcgggaaag
     6541 gtcgaggcct cgaccacaac tgctttcacc cgtctcatcc gcaacctgtc acgggtggag
     6601 ggcttcggcg atcggatcgt gccgatcgtt cccgacgagg cccggacctt cggcatggac
     6661 tcgctgttcc gcgagttgaa gatctacgcc tcccggggcc agctctacga atccgtggat
     6721 gcctccatgc tgctgtcgta caccgaggcc caggatgggc agatactgga ggaagggatc
     6781 accgaggccg gttcggtcgc ctcgtggatc gcagctgcca cttcctacgc cacccgcggc
     6841 ttgccgatga tcccgttctt catcttctac tcgatgttcg gcttccagcg ggtcggtgac
     6901 ttgatctggg ccgcggcaga ttcgagagcg cggggtttcc tgctgggcgc aacagcaggg
     6961 agaaccacgc ttcagggcga agggctgcag catcaggatg gtcatagcct ggtgctggcc
     7021 accaccgtcc cggtctgcga ggcgtacgac ccggccttcg cctacgaggt gggaaccatc
     7081 atcgagcacg ggttgcagcg gatgtatggg gcgggtgagg atcgcttcta ctacctgacc
     7141 ctgtacaacg agaactacgc ccagccgccg cgccccgacg gtgtgagcga cggcatcatc
     7201 gaagggctct accggtggca ggtcgcacca ggagacaacg ggccaaggac ggcaatcctt
     7261 ttctccgggt cggcgaatct ggctgcccgc gaggcggccg aggaattggc acagaactac
     7321 ggagtgcggg cggacctgtg gtcggcgacc tcctacaaga agctgcgcga ggaggctctc
     7381 ggcgtcgatc gatggaaccg gttgcaccct ggcgaaacgc cgcgggttcc gctggtcagc
     7441 cgccgcctcg ccgacgtcgg gggaccggtc atcgccgtca cggacttcat gaagttggtc
     7501 cccgatcagg tggcccagtg ggtgccgcag cggttcgtga ccttgggcac ggacggattc
     7561 gggcgctccg actcgagaca agccctgcgc aagttcttcg agatcgacgc cgggagcatc
     7621 gtggcggcaa ccctctgggc cctggccaat gacgatcttg tgcctcgaag cgcagtattg
     7681 gacgcgttca cccgctacgg gatcgatccg gaggctccgg acccggcgca tcagcattga
     7741 ctccggaagg gcccctggtg gtggtgatgc cggagccaat ccggcaggcg attctgaccc
     7801 acgccgagaa ctcgcttccc gatgagtgct gcgggctgat cgccagtgtg gagggtggta
     7861 tccgcttcgc ttacccgctg accaactcca acccatcacc tgtgtccttc accgtcgatc
     7921 ccggcgagca cttcggcgcc atgagccacg ccgaaaacca gggctgggag atctcgggtg
     7981 tctttcattc ccatccgacc ggcgagccgt caccctcggc gaccgacgtg gcgatggcct
     8041 gggatccgct ctggatccat ctcgttgtcg cccggggtga gatcgcggcc tttcgaatcg
     8101 tggatggtga agtggaggcc gtcgagctgt ggttggtggc aaaaggccca taagatccgg
     8161 tcctgccggg tagcgtcgcc gagggagccc gaggaggtca ccgttgcccc gaatcgaaga
     8221 gtctgccctt cccggagttg gggtcaggta cgagttcaag accgatgacg gtcggcgcat
     8281 aggggtggtc catcatcgga ccgggcgccg tgagctcttc gtctgcgagc ccgacgacac
     8341 cgatgccgcc gtggtcaaca tgagcctctc cgacgaggag agccatgccc tctccgaagc
     8401 tcttggcggg tcgaccgtgg tggcgaactt ggccaacctg cagcaacaag tcgagggcct
     8461 tgccatcgat tggctatcga tcgccccggg atccccctac gcgggcaaga ccatcgcgga
     8521 cgcccgcatc cgcacccgct ccggtgtatc ggtggttgct gtgctccgca gtggtgagac
     8581 ctttccctcc ccggacccag gctttgccat cgagcctggt gacaccctgg ttgtcgttgg
     8641 cacccccgac ggcatcaagc agacccaaga gatccttcgc accggctagc tcatggacga
     8701 aagcgccctc ttcctaatcg aactgggcgc cgtgatcctc gcgctcggtg tgatggcccg
     8761 tctcgcgggc cgtctcggga tctccccgat ccccctctac ctgctcgcag gcctcgctct
     8821 cggagagggg ggtgtcttcc cggtcatcac ctcggaggga ttcatcgaat tcggggccga
     8881 actgggtgtg atcttcctac ttttgatgct gggtctcgaa tattcggcgc gcgagttggt
     8941 gcaaggggtg cgatcagtag ccagcgtcgg tctggccgac ttcctgctca acttcacccc
     9001 aggttttgtc gccggaatcg tcctcgatct cggtctggtt cccgcggtgt tcttcggagg
     9061 cgtcacctat gtctcatcga ccgggatcgt ggccaagctt ctcgaagacc ttggctggtc
     9121 gggcaaccgg gaaacaccgg tggtgctttc gatcctggtc ttcgaagacc tggccatggc
     9181 agtcctcctc ccgattctcg gagttctggc gctcggatcg tcgatgtcct cggcggtgct
     9241 cacagtcggg ctcgcagtgg tggtcatcgt cgggttcatc ggcctcgtct cccggggtgg
     9301 ggatcgcctc ggacgactgg tgttccatca ctcagacgag gtgagcctgc tctccttgtt
     9361 cgggttgacc ctggtggtgg ccgggttggc ggaaggggtc aacatctccg cagccgtcgg
     9421 cgcattcctg gtcggaatcg gggtttccgg agattcagtg gaccgggccc ggtccctgct
     9481 caccccgatg cgcgacctgt tcgctgcggt cttcttcgtc ttcttcggcc tgcagaccga
     9541 ccccggtgac attcccgagg tggcggtggc cgcgatcacc ctggccatcg tgaccgggct
     9601 gaccaaggtc ctggttggtt ggtgggcggc cagccgggcc ggggtggggc gccagggccg
     9661 tctccgtgcc ggcatcctcc tggtggcgag aggcgagttc tcgatcgtga tcgctggtct
     9721 cgcggtttcg gtcgccggtg gtagccagtt gaagcctctg gccgcgacct atgtgctcat
     9781 catggcgatc gccgggccta tcgccgccaa ggttgcagat gcacgccgca aacacgtgat
     9841 cctcgtctga agaccctgcc tcagacggcg gcgaaccaac ccattgcccc ccgctcggcg
     9901 agccagtgct gatggggatg gaacatgtag cgcccgagac tgggcagggt gaattcgatc
     9961 atggctcgct gcgcctgacc gaggacgatg gtgtcgtccc gaaaggccgg cgtgcccccc
    10021 atgcctgccg gatagacatc aaaagtctca gcatggaggt ggaacgaagc aatcggttcg
    10081 tgttccagca tgttgaccac gaaggcccga acgggttctc cgaccggcac cttgattgga
    10141 taggagttgt agaaaccggc gactccgttc caggcgatca cgccgttgac tcctagcgct
    10201 tcgctgctga acccggagac aagcagggcg acctcagtag ccggcgcccg ctcctctaca
    10261 ggatccacaa tcatcatccc gtagaggccg cgcccgatgt gctcggcaag tggagtggtg
    10321 tgacagtggt agggatggac tccgacgggg ccggcttcga tctcataggt ggtcttccct
    10381 cccggaggca cctgctccca accgtcctgt tcgggccggt gagcaccgtg aaagtgcagg
    10441 ttgtgcgggt gaccggtccg attctcgaag ctgatcctca atgtgtcgcc ttccgtggcg
    10501 cggatgattg gcgccggaac ggtgccgttg taggtccaag cctcgaacaa caccccatca
    10561 ccgacctcga tcaacctgtc ttcgacggct agctccaact cgcgaaccac tccggccggc
    10621 tgaccgtccc gcggcggggg aaaagtctga tggtcgaggt cgccagggcc cagagaagtg
    10681 ggtggtgagt gcaggttgct gtacttgccg tcgtcgctct cgcccggcat tgcatgctca
    10741 ctccccgggt cgtagtcctc ggtgtcggca gcctgcgctt gatgctggat cgtgtgcccg
    10801 aggacggatc ctccgaggaa gacggcgccg cccaacccca ccttcttcag caaatcgcgg
    10861 cgggacacac cttcgggcat cggtcgactc agacgatgcc cccgacgacc atccccaggg
    10921 ccaggaagaa gaggtagacc gccattcctc ccgccaggaa ggtccacctt tgccagaccg
    10981 ggatggtgct cttgccgaac aggtagtagg cgatcacacc gaggaatggg atgaccagga
    11041 tggctcccat ccaaccaatt gccttgcccc gactcacatc ctcacggcgg gagaggtccc
    11101 agattgcgat caccacccaa gacgtataga ggaccagcgg aaggacatat gcatagaggc
    11161 cgaagaagac cccggcggcc gaagccaagc tgacacccat gatgttgatt cctctcttct
    11221 gtgtgacttc caaccctgga ggcatctccg gggtagtaat gctgtcgagg tcgggtttcg
    11281 acccggcaaa gacgttgacc gcggggatga ccggggcatc agcgccaccg ggcatgctgg
    11341 gctgccgctc cggttcgggc accgcagcag ggtcgaattc gggctggagg ccgacaccgc
    11401 tctcgaatag ccgtcccaat tgggcgctgc tcgagccaaa ctcgaaaagc gcagggagcc
    11461 gaagtccctc acagggctgc ttcaactcga gagcagcggg caaggtcgat gtgtactcgt
    11521 tgtcctcgaa gcagttgccc gagtcggccg gacccccgag ggtgaggtct gccagccccg
    11581 acccctcgac gacgttgccc tcgacgtggt ggtcgaaaga catgtacatg ttggcgtcga
    11641 gcatggggag gatgactatc ccgctctgtg agtggttgaa cacccgattc ctcgccacgt
    11701 aggcgtcgcc ccctcccgcc aacatgatcc cattgccctg agccgcccat tgcagggcct
    11761 tggcgggagc cttcacattg ccattgtcgt agaccagatt ccccaccacg gtcacaccgc
    11821 gagaaggggg cagaagctca ctatcgagcg tgttgggagc gatcccggac aggttgtacc
    11881 gccacaccga gttgacgatg tacgtgtcgt tcgaagcatt ggtgcctgag aagccaagcc
    11941 cgttccattc ggcgatcgaa tcggtgatcg tcgcctgaca tgggtcgcat tgcccgacgt
    12001 agaacccagc gtcgggagag ccaaacgcgt atgagaattc catcagcccg tcactggagt
    12061 cgaaggcgta gatcccgtag tcgttgttgt tgagggcagt caggtacgac ccgcgaaagc
    12121 ctcgaacccc ggtccagaac acaccattgc cggtggcgtt gcgcacagtg aggttctcga
    12181 cggcgacgcc gtccgcaaag aacacctcga cgccgttggt ccgctgcatc tcaccgtcga
    12241 tgatcacgtc gttgcgatcg acaccccgga tcgtgagccc gggcgtcgtg acttccactt
    12301 cttccaggta gaccccgcgg tcgatgagta cgagatcgcc gggatccgcg gcgtcgaccg
    12361 ccgactggat ggtcgggtag tcctggggaa cgagacgggt ctcccccgac cactccatgg
    12421 gcgaatcggt cgcagggtcc ccgagcgcgg ccggcgaagg gtcgccgaca ggcgggccgg
    12481 cacaggcagc aaggccgatc acgaggacca ccaggagccc ccgtcggcgg ctcacccttc
    12541 cagcgcctcc acaatcacgg ttcccgccat gcccgagcct tcagcattgc catggaacgt
    12601 gcagaagaac ttgtactcac ccggctgatc gaaatcgatc gtggc
//
LOCUS       DBNHXU010000004         1543 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4366425, whole genome shotgun sequence.
ACCESSION   DBNHXU010000004 DBNHXU010000000
VERSION     DBNHXU010000004.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1543)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1543)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1543
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4366425"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /gene="manB"
                     /locus_tag="AC161X_00090"
     CDS             <1..479
                     /gene="manB"
                     /locus_tag="AC161X_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003941022.1"
                     /note="converts mannose-6-phosphate to
                     mannose-1-phosphate; the resulting product is then
                     converted to GDP-mannose by ManC which is then used in the
                     synthesis of mannose-containing glycoconjugates that are
                     important for mediating entry into host cells; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HIR4987976.1"
                     /translation="IAEAGGRAIRTRVGHSYIKQVMAESGAVFGGEHSGHYYFRDNFN
                     ADSGMLAMLILMRVMTEDGRPLSELRRDVERYAASGEINLTVRDPLASIEVAAKTYAS
                     ATQDRLDGLSIDLGWAWFNLRASNTEPLLRLNVEAPDADSVAKLVNEIERLVEEVP"
     gene            476..655
                     /locus_tag="AC161X_00095"
     CDS             476..655
                     /locus_tag="AC161X_00095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HIR4987977.1"
                     /translation="MSLIDPTLAEILVCPVDHSDLDQDEAASRLVCTECGRRYPVRDG
                     IPVMLVEEAEGGPDA"
     gene            648..>1543
                     /locus_tag="AC161X_00100"
     CDS             648..>1543
                     /locus_tag="AC161X_00100"
                     /inference="COORDINATES: protein motif:HMM:NF021916.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HIR4987978.1"
                     /translation="MPDMIDQIRSLGDQLRWAANVEVPDIEPTSDVLVAGMGGSGIAG
                     DFAAALAVSGAGRVAVQKGYGPIPGWARERKPLLIAISFSGNTEETLDVVRSAIGSDL
                     PVVTLTAGGELGRITAERGWPSIAVPGGTQPRAAVGYLFGAVARVVSAAGIIPDQGPA
                     LLEAAEAADRATTEGSPRWELATGISEGLAGRIPIIYGGGPISGAVAQRWKTQINENA
                     KTPAWWSILPELDHNEIVGWEAMPHLTRESLGIVALTEGADHPRVKDRLAHSRALTEA
                     AVPWVAEVAAEGDTPLARLVSLV"
BASE COUNT          285 a          442 c          533 g          283 t
ORIGIN      
        1 tcatcgccga ggcgggaggg cgagcgatca ggacgagggt gggtcatagc tacatcaagc
       61 aggtgatggc cgaatcgggt gcggtgttcg gtggggagca cagcggccat tactacttcc
      121 gtgacaactt caacgccgac tcagggatgt tggcgatgct gatcctgatg cgggtcatga
      181 ccgaggatgg aaggccacta tccgagctca gacgcgacgt cgagcgttac gccgcctcag
      241 gggagatcaa tctcacggtg cgtgaccccc tggcctcgat cgaggtggcg gcaaagacct
      301 acgcctccgc cactcaggac cggctcgatg ggttgagcat cgaccttgga tgggcgtggt
      361 tcaatctgcg ggcgtcgaac accgagccac ttctccggtt gaatgtggag gcaccggacg
      421 cggattccgt tgcgaaactc gtgaacgaga tcgagcgact tgttgaggaa gtgccatgag
      481 cttgatagac cccaccctcg ccgagatcct ggtgtgcccc gtcgaccact cggatctcga
      541 tcaggacgag gcggcctcgc gcctggtttg caccgagtgc gggcggcgat atccggttcg
      601 ggacggcatc cccgtgatgc tggtcgaaga ggccgaagga ggccccgatg cctgacatga
      661 tcgatcaaat tcggagcctc ggggatcaac ttcgctgggc agccaatgtc gaagtgcccg
      721 atatcgaacc gacctctgac gtcctggttg cagggatggg agggtcaggc attgccggcg
      781 acttcgctgc agccctcgcc gtatctggag cggggagagt cgccgtgcag aagggatacg
      841 ggccaattcc cggttgggcc cgggaacgca agccactgct gatcgcaatc tcgttttcgg
      901 gcaacacgga ggaaacgctg gatgtcgtgc ggtctgctat cgggagcgac ctgcccgttg
      961 tcaccctcac cgctggtggt gaattgggtc gtatcaccgc cgagcgaggc tggccgagca
     1021 tcgctgttcc tggtgggacc cagccgcgag ctgcggtggg ctacctattt ggagcggtcg
     1081 cccgtgtggt gtcggccgcc gggatcattc ccgatcaggg cccagcgttg ctggaggccg
     1141 ccgaggctgc cgaccgggca acgaccgagg ggagcccgag atgggaactg gcaactggga
     1201 tttcggaggg actggctggg cggattccca tcatctacgg aggcgggccg atcagcggag
     1261 ccgtggccca acgttggaag acccagatca acgagaacgc gaagactccg gcttggtggt
     1321 cgattctccc tgagctcgat cacaacgaga tcgtggggtg ggaggcgatg ccccatctga
     1381 ccagggagtc tctcgggata gtggccctca ccgagggtgc cgatcatcca agggtgaagg
     1441 atcggctagc ccactcccgg gctctcacag aagctgcggt tccctgggtt gccgaagtcg
     1501 cagcggaagg cgacactcct ctcgccagat tggtgtcttt ggt
//
LOCUS       DBNHXU010000005         2489 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3969901, whole genome shotgun sequence.
ACCESSION   DBNHXU010000005 DBNHXU010000000
VERSION     DBNHXU010000005.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3969901"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..>2489
                     /locus_tag="AC161X_00105"
     CDS             137..>2489
                     /locus_tag="AC161X_00105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4987979.1"
                     /translation="MTLQRRAGQGFPWPTFLILAGISLVILIVLNPGWILSATTPTGG
                     DMGAHVFGPAYLRDHLLPHGRILGWSNDWFAGFPAFYFYFPLPSLVIVLLDVLLPYGV
                     AFKVVTVIGLLGIPPAAYFLGRSMLLGRHVSIVAAGAGAVFAFLESYSIYGGNVASTL
                     AGEFAYSWSFALSLVYLAFLIKAVREDKKYVKWAALAFAAMLLSHVLTTIVIVFASLF
                     VLPWRKGFWRTLAIGFWGFAMAAFWAVPLAARIGLTSDMAWSPLTRWEEVFPVELWLL
                     IPIAIPGAIWASRHTSRAGPLMAATLLPLIYYPLPTVLPELFPTLFGGERWKLWNGRL
                     LPYWYFGVAFFAAVGVGAAVVWASRRLPSRISGHWARLLVVVVTVVAAFLVADAADFP
                     AWAWIPVALAGAGLLALSFFWSSDLSTRQFLTAFATVVIVFGALAGVTFIDGWSKWNY
                     EGYESKKPWPEYEALMVELDKLPDGRVHWEANNEALNRYGTPMSPMLIPYWTEGSQKS
                     MEGLFFESSLTTPFHFINSSEMSEKPSNPIPGLTYHTGEMDRGIKHLELYGVSYYVSV
                     TPESTAKADAMPELTKLATTAPFTIYALPETGLVEAASYVPSVYDVGERGLLALLTGH
                     ATVTDENGVTLPSFYDMSLEWYEDINHMDRWVVADGPAEWPRIESLTERPAEQLDVPA
                     DAVSDVVVEDHRISFHTTAVGVPHLVKVSYFPNWVAEGAEGPWRAAPSLMVVVPTSED
                     VVLEFKNTWAETGGMVISAVGIVVLIGAGVVGFRRKKAALSAQS"
BASE COUNT          390 a          762 c          774 g          563 t
ORIGIN      
        1 agatcgagca tctcctgccc gacggcaccg aggtccgctt cttcgcctgt cacaactgtg
       61 aggagaagtg gtggaacaag gacggtgttg acatcgacgt cgcccaggtc ttggagatcg
      121 tccgccagca tcgcgcatga ccctccaacg gagggccggc cagggcttcc catggccgac
      181 gttcctcatt ctcgccggga tctctttggt gatcctgatc gtcctcaatc ccgggtggat
      241 cctttctgca accactccca ccggtggaga catgggggcc cacgtctttg ggccggcata
      301 cctccgagac catctgcttc cgcacggccg gatcctcggt tggtcaaatg actggtttgc
      361 gggctttccc gccttctact tctacttccc gcttccctca ctggtcatag tcctcctcga
      421 cgtgctcctg ccctacggag tggcgttcaa ggtcgtcaca gtgatcggcc tccttgggat
      481 ccccccggcc gcttacttcc tgggtcggtc gatgctgctg ggacgtcacg tctcgatcgt
      541 ggccgccggc gccggagccg tgttcgcctt tctcgagagc tactccatct acggcggcaa
      601 cgttgcgtcg accctggccg gcgagttcgc ctattcatgg tcctttgccc tttccctggt
      661 ctacctggca tttctgatca aggcggtgcg agaggacaag aagtacgtga aatgggcggc
      721 gctcgccttt gccgcgatgc tgctctccca cgtcctcaca acgatcgtga ttgtgttcgc
      781 atcgctattc gtccttccgt ggcgaaaggg cttctggcgg accctggcga tcggtttctg
      841 ggggtttgcc atggcggcgt tctgggcggt gcccctcgcg gcccggatcg ggttgacctc
      901 ggatatggca tggtctccgc tcacccgctg ggaggaggtc ttcccggttg agctgtggct
      961 cctgatcccg atagcgattc ccggtgcgat ttgggcaagc cgtcacacat ctcgggcggg
     1021 gcccttgatg gcagcgaccc tgctgccttt gatctattac cccctcccca ctgtcctgcc
     1081 cgaattgttc cccaccttgt tcggtggcga gcgttggaaa ttgtggaatg ggcgcctgct
     1141 cccgtattgg tacttcggtg tcgctttctt cgccgcagtc ggcgtgggag ctgccgtcgt
     1201 ttgggcttcg cgacggctcc cgtcgcgaat ttccgggcac tgggctcggc tactggtcgt
     1261 ggtcgtcacc gtcgtcgccg ccttcttggt ggcggatgct gccgactttc cggcttgggc
     1321 gtggataccg gtggcccttg ccggcgcggg gttgcttgca ctgtcgttct tctggtcgtc
     1381 cgacctgagc acgcgtcaat tcctgaccgc ctttgccacg gtggtgattg tgttcggtgc
     1441 cctggccggg gtgacgttca tcgatggctg gtcgaagtgg aactacgagg ggtatgagtc
     1501 gaagaagcca tggcccgaat acgaggcgct gatggtggag ctcgacaagc tcccggatgg
     1561 gcgggttcat tgggaagcca acaacgaggc gctcaatcga tacgggacac cgatgtctcc
     1621 gatgctgatc ccatattgga ccgagggctc ccagaaatcg atggagggtc tgttcttcga
     1681 gtcatcgctt acgacaccat tccatttcat caactcctcg gagatgtcgg agaagccttc
     1741 caaccccatc cccggcctga cctatcacac aggtgagatg gacaggggga tcaagcacct
     1801 cgagctgtac ggggtgtcgt actacgtatc agtgacgccg gagtcgacgg ccaaggcgga
     1861 cgctatgccc gagctcacca agctcgccac caccgcgcca ttcacgatct acgcgctgcc
     1921 tgagaccggc ctggtcgaag cggcgagcta cgtgccctcg gtgtatgatg ttggcgagag
     1981 gggccttctg gctttgttga ccgggcacgc caccgtgacc gacgagaacg gtgtgaccct
     2041 ccccagcttc tatgacatgt cgctggagtg gtacgaggac atcaaccata tggatcgctg
     2101 ggtggttgcc gacgggcccg ccgaatggcc ccgcatcgag tcactgacgg aacgcccagc
     2161 cgaacagttg gacgtcccag cggatgcagt atcagacgtc gtcgtcgaag accatcgaat
     2221 cagttttcac accaccgccg tgggcgtgcc tcaccttgtc aaggtttcct attttccgaa
     2281 ttgggtagcg gaaggagcgg agggcccgtg gcgcgccgcg ccatcattga tggttgtagt
     2341 cccgactagc gaggacgtgg tcctcgagtt caagaacacc tgggccgaga ccgggggcat
     2401 ggtcatctcg gcagttggga ttgttgtgct gatcggcgcc ggcgtggtcg gcttcaggcg
     2461 gaagaaggct gcgttgagcg ctcagtccg
//
LOCUS       DBNHXU010000006         6385 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5359008, whole genome shotgun sequence.
ACCESSION   DBNHXU010000006 DBNHXU010000000
VERSION     DBNHXU010000006.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6385)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6385)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6385
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5359008"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..120
                     /locus_tag="AC161X_00110"
     CDS             <1..120
                     /locus_tag="AC161X_00110"
                     /inference="COORDINATES: protein motif:HMM:NF049491.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PaaD-like zinc ribbon domain-containing protein"
                     /protein_id="HIR4987980.1"
                     /translation="CPQCAAGAPRMVSRFGSTACKALMVCSQCGEPFDWFKEF"
     gene            127..1200
                     /gene="paaE"
                     /locus_tag="AC161X_00115"
     CDS             127..1200
                     /gene="paaE"
                     /locus_tag="AC161X_00115"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaE"
                     /protein_id="HIR4987981.1"
                     /translation="MTPVEFHPLRVVEVTSLTDESVVVSFDVPPSLTRVFKFLPGQHV
                     TLRAVVDGEDLRRSYSICANANSGKLRVGIKRLPGGLFSTWATTRLRAGEVIGVMPPV
                     GEFTIKPDPGRAGHRVAIVAGSGITPVLSLVATTLELEPGSSWTVIFGNRTVNSVMFL
                     EELEALKDRYPARLQLFHLLSREGSDLALLSGRIDPGKIEMIQSRLLDERPVDGWFLC
                     GPYEMVTMARETLTRLGVAGELIHDELFFAGPLDLSSLPPEPPPGEGSVQLTVILDGR
                     AVETLMTPETSILDAALRVRSELPFSCKGGMCATCKGRIEEGSVTMSKNYALVDAEIE
                     AGYVLTCQAHPTEDRVVVRYDHR"
     gene            1200..1778
                     /locus_tag="AC161X_00120"
     CDS             1200..1778
                     /locus_tag="AC161X_00120"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR4987982.1"
                     /translation="MVTVRNTRQDVVAAAGRLFAENGYHGTSMRDLGRELGLLGSSLY
                     AHVDSKQDLLVEVVEEGARLFQASADAALATEGSAANKLHRLIAGHVGVVVDNPDVVR
                     TYLNEARMLDPEHRGRVIAARDGYEQAFRRVIALGSSDGSLRPDADPKLGSIFLLSIL
                     NAIERWYHPEGEVGVTELVDEVSEFALGALRP"
     gene            complement(1768..2904)
                     /locus_tag="AC161X_00125"
     CDS             complement(1768..2904)
                     /locus_tag="AC161X_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4987983.1"
                     /translation="MSNNEGGTTEQQKPSKGRAIATIVLVVLSSLSVVLAVVASWAHN
                     TLLETDQFMEAVGPALEEPALHTALGDKVSTEVVTALDLETRIGTALAQVDDFLFGSL
                     IDALDLGPRGQQILEAYNGPNLESLAPTIASGLEERVTTRIQDFFASPDFGSRLPGLV
                     ERAHEATIALLRDEFTEIPNVSIVAGEVQLNLIPIIADAIRQVLPDLGDLGSNINLPD
                     RLSEAVAEGRQQLADAIGAQLPDDFGQVTIMSEDRLVELQNGVVMLDRLVWLLIGLAI
                     VLIVVTILASETRRRTAIQLAIGVVIALLVAGLVLNAVEDRVIEAIDNPNGQTAAAAI
                     MGDVFSKLWSIGLQVGSLALILGVVAFLAGVSSKAGNTDETIKV"
     gene            3010..3426
                     /locus_tag="AC161X_00130"
     CDS             3010..3426
                     /locus_tag="AC161X_00130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005518648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7144 family membrane protein"
                     /protein_id="HIR4987984.1"
                     /translation="MSHQAGNGYDGPPAMAIGAIMFAGIMMMMSGVFSAFSGIVALAK
                     DDFYVVLPNYLLELDPTTWGYVHLVLGIVVALAGFFVLTGRTWARVIGIIVALVSAIA
                     NFGFIPFYPVWSLLIIAVDIVVIWALAAHGRAMSQA"
     gene            complement(3497..4525)
                     /locus_tag="AC161X_00135"
     CDS             complement(3497..4525)
                     /locus_tag="AC161X_00135"
                     /EC_number="1.2.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:ferredoxin oxidoreductase subunit
                     beta"
                     /protein_id="HIR4987985.1"
                     /translation="MTAPTYTRADFQTDQEVRWCPGCGDYTILASVQQFLAGLGRPRE
                     EFVFVSGIGCASRFPYYMNTYGMHSIHGRAPAIASGVATTRPDLSVWVITGDGDGLSI
                     GGNHLIHALRRNINLKILLFNNQIYGLTKGQYSPTSELGKQTKSTPFGSIDYPFNPVS
                     LALGAEATFVARTLDMDRSHTEGLLQAAYEHEGSAFIEIYQNCNVFNDKAFIQLTGKE
                     ERVHNRIDLEPGKPIVFDEGAKAVVMRNGQATVVDSASVDPGAILVHDPTRPDPSIAF
                     ALSRLSHGPYGPTPLGIFRSVKRPVYESSMIAQIETAKEKAGEGDLAKLIASHGTWTV
                     KDPNANGG"
     gene            complement(4522..6381)
                     /locus_tag="AC161X_00140"
     CDS             complement(4522..6381)
                     /locus_tag="AC161X_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase subunit alpha"
                     /protein_id="HIR4987986.1"
                     /translation="MATAKPPTPDTETFDKIVIRFAGDSGDGMQVAGSRFTDASALFG
                     NDLATLPSFPAEIRAPAGTLPGVSSFQVQIADFDILTAGDQPDCLVAMNPAALKKELK
                     DLRQGGTLVVNSDAFDERNLAKAGYTANPLDDGTLDGFRVISVPMDELTKEAVKDSGV
                     SGRSVLRSKNFLALGLLAWLFHRPTEPTVEWARKKFAKTPEVANANIAAFQAGYTLGE
                     ATEAAKTTFVIKPAALEPGTYTNINGNSALAWGLIAASKLSGLPLFYGSYPITPASDI
                     LHELSRHKNFGVRTFQAEDEIAGVGSAIGAAFAGSLAVTGSSGPGIALKSESISLAFM
                     MELPLIVINVQRGGPSTGLPTKPEQSDLLFSLYGRHGESPIPVIAAASPSDAFAMAIE
                     AARLALKYMTPVLLLSDNQIANGSEPWKLPDVSTLAPIPVEFAKYANGPDGVFLPYMR
                     DIDTFARPWAVPGTPGLEHRIGGLEKEAISGNVSYEPANHQLMTDTRAWKVANIANDI
                     PPVEVAGDTDAEILVLGWGSTYGSVRAAARRVRLAGKKVATAHLRYLNPLPINLGEVL
                     NSYKQILIPELNTGQLRRVIRAEFLLDVKGLNKVAGEPFKVAEIEEKILEMIK"
BASE COUNT         1079 a         1922 c         2079 g         1305 t
ORIGIN      
        1 tgcccccagt gcgcggcggg ggcgccacgg atggtttcga gatttgggtc caccgcatgc
       61 aaggcgctca tggtgtgttc gcaatgcggt gagcccttcg attggttcaa ggagttctga
      121 gcgatcatga ccccggtcga gttccacccg ttgcgtgtcg tcgaggtgac ctcgcttacc
      181 gatgagtctg tcgtcgtcag cttcgacgtg cccccgtcgc tgacccgggt gttcaagttc
      241 cttccgggac agcacgtcac gcttcgagcc gtcgtcgacg gcgaggatct gcggcggtcc
      301 tattccatct gtgccaacgc caacagtggc aagctcagag tcggcatcaa gcgacttccc
      361 ggaggcctct tttcgacttg ggccacgacg aggctcaggg cgggggaggt catcggggtc
      421 atgcccccgg ttggcgagtt cactatcaag cccgacccgg gccgtgcggg acatcgcgtg
      481 gcgatagtgg ccggatccgg gataaccccc gtgttgtcgc tcgtggccac cactctggag
      541 ttggagcccg gctcatcgtg gactgtcatc ttcgggaacc ggacggtcaa cagcgtgatg
      601 ttcctcgagg agttggaggc actcaaggat cgctacccgg cccgtctcca gctcttccat
      661 ctcctgagtc gagagggatc cgacctcgcc ttgctctcgg gtcgcatcga tcccgggaag
      721 atcgagatga ttcagagccg gctgctcgac gaacgcccgg tcgatgggtg gttcctctgt
      781 gggccgtacg agatggtcac gatggcccgg gagaccctta cccgcctcgg agttgcggga
      841 gagctcattc acgacgagtt gttctttgcc ggccccttgg acctttcgag tcttcccccg
      901 gagccgccgc caggagaggg gtcggtgcag ctgacagtca tcctcgatgg gcgtgccgtg
      961 gagacgctta tgacccccga gacgtcgatc ctcgatgccg ctctccgggt tcgatccgag
     1021 ctgcctttct cgtgcaaggg cggcatgtgc gccacttgca agggacggat cgaggagggc
     1081 agcgtgacca tgtccaagaa ctacgccctg gtcgacgccg agatcgaagc cggttatgtg
     1141 ctcacgtgtc aggcgcaccc aacggaagat cgtgtcgtag tccggtacga ccaccgctga
     1201 tggtcactgt ccgcaacacc cggcaggatg tggtggcggc cgcggggcgt cttttcgccg
     1261 aaaacggata ccacgggacc tcgatgcgtg acctggggcg ggagctgggc ctgctcggct
     1321 cctccctcta tgcccacgtc gactcgaaac aggatctcct cgtcgaggtc gttgaggagg
     1381 gagcacgcct gttccaggcg tcggccgatg ccgcgctggc gacagaaggt tccgccgcca
     1441 acaagctgca tcggctgatt gctggtcatg tcggggtggt agtcgataac ccggacgttg
     1501 tccgcaccta tctcaacgag gctcggatgc tcgatccgga gcatcgaggg cgggtgatcg
     1561 cagcccgcga cggttatgag caggccttcc gacgggtgat cgccctcggg agttccgacg
     1621 gatcgctgcg tcccgatgcg gatcccaagc tgggctcgat cttccttctc tcgatcctca
     1681 atgccatcga gaggtggtac caccctgagg gggaggtcgg tgtcaccgag ctggtggacg
     1741 aggtgagcga gtttgccctg ggtgccctca gaccttgatg gtctcgtcgg tgttgcctgc
     1801 ttttgaggag actcccgcga ggaaggcgac tactccgagg atgagtgcca gcgatccaac
     1861 ttggagcccg atcgaccaca gcttggagaa gacgtcaccc atgattgctg ctgccgcggt
     1921 ctggccattc gggttgtcga tggcctcgat caccctgtcc tccaccgcgt tcagcaccaa
     1981 tcccgccacg agaagcgcaa tcaccactcc aatcgccagt tggatagcgg tccttcttcg
     2041 ggtctcggaa gccaagatcg tcacaacgat caacacgatg gccagtccga tcaatagcca
     2101 aaccaaccgg tccagcatca ccaccccatt ctgaagctcg acgagccgat cttcggacat
     2161 gatcgtcacc tggccaaaat cgtcggggag ctgggcgcct atcgcgtctg ccagctgctg
     2221 acgcccttca gcgaccgcct ccgagaggcg atcggggagg ttgatatttg atcccaggtc
     2281 gccgaggtcg gggagcacct gtctgatcgc atcggcgatg atcggaatca ggttgagctg
     2341 aacttcgccg gcgacaatcg acacattcgg gatctcggtg aactcgtcgc ggagcagcgc
     2401 gatcgttgct tcgtgggctc gctccaccag tcccggcagt ctcgagccaa agtcagggga
     2461 cgcgaagaaa tcctggatcc gggtggtgac ccgttcctcg agcccggagg cgatcgtcgg
     2521 ggcgaggctt tccaggttgg gcccgttgta ggcctcgagg atctgctggc cccgcggacc
     2581 gaggtcgagg gcatcaatga gggagccaaa taggaagtcg tcgacctggg cgagggcggt
     2641 cccgatcctg gtttctagat cgagggcggt tacgacctct gtgctgacct tgtctcccag
     2701 ggctgtgtgg agggccggtt cctctagggc gggcccgact gcttccatga attggtcggt
     2761 ttcgaggagt gtgttgtggg cccaggaggc gaccacggcg agaacgacac tcagcgagct
     2821 gaggacgacg agcacgattg tggcgatggc ccggcctttg ctgggcttct gctgttcggt
     2881 tgtcccgccc tcgttgttgg acatgttctc tcgactcggg tgcgtcacgg cactctaggt
     2941 tgccaaacac cccgggaagc tgtcgtgtag gtttccggtc cacaacggac ctcatcgaaa
     3001 ggaaagagga tgtctcacca agcgggcaac ggctacgacg ggcctccggc catggccatc
     3061 ggggccatca tgttcgccgg aatcatgatg atgatgagcg gggtgttctc cgccttttcg
     3121 ggaattgtcg ccctggccaa ggacgatttc tacgtggtgc ttcccaatta cctgctcgag
     3181 ctggacccaa caacctgggg ttacgttcac ctagtactcg ggatcgtggt ggctttggcc
     3241 gggttcttcg tcctcaccgg caggacctgg gcccgggtga ttggaatcat cgtggccttg
     3301 gtcagcgcca tcgccaactt cggcttcatc cccttctacc cggtctggtc gctcctgatc
     3361 atcgccgtcg atatcgtcgt gatctgggcc ttggccgccc acggacgcgc catgtcccag
     3421 gcctaggcag caactccact cattttgtga atgcagggcg tcacggattc gtgacgccct
     3481 gccttaggaa ttgggctcag ccgccgttgg cgtttgggtc cttgaccgtc caggtgccgt
     3541 gactggcgat cagcttggcc aggtcaccct ctccggcctt ctctttggcc gtttcgatct
     3601 gggcgatcat cgacgactca tacaccggtc gcttcacgct tcggaagatt ccgagtggtg
     3661 tcggcccgta gggaccgtgt gacagccggc tgagggcgaa tgcgatcgat gggtcgggcc
     3721 gggttgggtc gtggaccagg atggccccgg ggtcaacaga tgcgctgtcg acgaccgtcg
     3781 cctgcccgtt gcgcattacc accgccttgg cgccttcgtc gaagacgatc ggcttgcccg
     3841 gctcgaggtc gatgcggttg tggacccgct cctccttgcc ggtgagctgg atgaatgcct
     3901 tgtcgttgaa cacgttgcag ttctgataga tctcgatgaa tgccgagcct tcatgctcgt
     3961 aggcggcctg gagaaggccc tcggtgtgac tgcggtccat gtccagggtt ctggccacga
     4021 atgtggcttc agcccccagc gccagagaga ccgggttgaa cgggtagtcg atcgagccga
     4081 acggtgtcga cttggtctgc ttgcccagct ccgaggtcgg cgagtactgg cccttggtca
     4141 acccgtagat ctggttgttg aacaacagga tcttcaggtt gatgttgcgg cgcagcgcgt
     4201 gaatgaggtg gttgccgccg atcgataggc cgtcgccgtc tccggtgatc acccaaaccg
     4261 acaggtcggg gcgggtggtg gccactccgg aggcgatcgc cggcgcccgc ccgtggatcg
     4321 agtgcatccc gtaggtgttc atgtagtacg ggaatcggga tgcgcacccg atgcccgaca
     4381 cgaacacgaa ctcctcccgg ggacgcccca atccggcaag aaactgctgg accgaggcga
     4441 ggatggtgta gtccccgcaa ccggggcacc accgcacctc ctgatcggtc tggaagtcgg
     4501 cgcgggtgta ggtgggggcg gtcacttgat catctccagg atcttctctt ctatctcggc
     4561 gaccttgaac ggctccccgg ccactttgtt caggcctttg acgtcgagca ggaactccgc
     4621 ccggatcacc ctcctcaact gaccggtgtt cagctcaggg atcaggatct gcttgtagga
     4681 attgagcacc tcgccgagat tgataggtag agggttgagg tagcgaaggt gggcggtcgc
     4741 caccttcttg cccgcgaggc gcacccgtcg ggccgctgcc cggactgacc cgtaggtcga
     4801 tccccacccc aggaccaaaa tctcggcatc cgtgtcaccg gcgacctcga ccggtgggat
     4861 gtcgttggca atgttggcca ctttccaggc ccgggtgtcg gtcatgagct ggtgattggc
     4921 cggctcatat gacacgtttc cgctgatggc ttccttctcc agacctccga tccgatgctc
     4981 cagaccggga gtgccgggga ccgcccaggg tcttgcgaag gtgtcgatgt cccgcatgta
     5041 ggggagaaag accccgtcgg gcccgttggc gtacttggcg aattccaccg ggatgggtgc
     5101 cagggtgctg acgtcgggca gcttccaggg ctcagagcca ttggcgatct ggttgtcgct
     5161 gagaagcaag accggtgtca tgtacttgag ggcgagccgt gccgcttcga tggccatcgc
     5221 gaaggcatca ctgggtgagg ccgcagcgat cacggggatc ggggactcgc catgacgccc
     5281 gtagagcgag aacaagaggt ccgattgctc cggcttggtc gggagcccgg tcgacggccc
     5341 tccccgctgg acgttgatga cgatcagcgg tagctccatc atgaaggcca gcgagatcga
     5401 ctcactcttc agtgcgatgc ctggtccgga tgacccggtg acagcgagtg agccggcgaa
     5461 cgccgccccg atggccgacc cgaccccggc gatctcatcc tcggcctgga aggtccgcac
     5521 cccaaagttc ttgtggcgcg acaactcatg gagaatgtcg gacgccggtg tgatcggata
     5581 ggacccatag aacaggggca aacctgacag cttggaggct gcgatcagac cccatgccag
     5641 tgccgagttg ccgttgatgt tcgtataggt cccgggctcg agcgcagcgg gcttgatgac
     5701 gaaagtcgtc ttggcggctt cagtcgcctc accaagggtg tatccggcct ggaacgccgc
     5761 gatgttggcg ttggcgactt ccggggtctt ggcgaacttc ttccgggccc actcgacagt
     5821 tggctcggtg gggcgatgga acagccaggc caggaggccg agggcgagaa agttcttgga
     5881 gcgaagcacg cttcgcccac tcacgccgga gtccttgact gcttccttgg tcagctcatc
     5941 catcggcacc gagatgactc gaaagccatc gagggtcccg tcgtcgaggg ggttcgccgt
     6001 gtagccggcc ttggccaggt tccgctcgtc gaaggcatcg ctgttgacca ccagggtgcc
     6061 tccctggcga aggtctttga gctccttttt cagcgccgcc gggttcatcg ccaccaagca
     6121 gtcgggctgg tccccggccg tgaggatgtc gaagtcggcg atctggacct gaaagctcga
     6181 taccccggga agggtcccgg caggtgccct gatctcagca gggaacgacg gcaaggttgc
     6241 caggtcgttg ccgaataagg cactggcgtc ggtgaatctg gatccggcta cttgcatccc
     6301 gtcgccggag tcgccggcga atcggataac tatcttgtca aaggtctcgg tatcgggggt
     6361 cgggggtttt gctgtggcga tttgc
//
LOCUS       DBNHXU010000007         3494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2779660, whole genome shotgun sequence.
ACCESSION   DBNHXU010000007 DBNHXU010000000
VERSION     DBNHXU010000007.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3494
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2779660"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..491
                     /locus_tag="AC161X_00145"
     CDS             <1..491
                     /locus_tag="AC161X_00145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4987987.1"
                     /translation="FEGRIAEELGRLSGIGQIRVIDAVVVTREGDDVAVLSVSDLDDE
                     ERAELRTVVGALIGLGVAGIDGAVAGAEAGASIDVDALTAAEMVGASLIEGLPDGSSA
                     LVLAIEHLWAIPLRDAVRDAGGVVMAHRSLTAEDLIAMGMAIGMEAEMDMEKEELADS
                     EA"
     gene            complement(569..641)
                     /locus_tag="AC161X_00150"
     tRNA            complement(569..641)
                     /locus_tag="AC161X_00150"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(606..608),aa:Arg,seq:cct)
     gene            complement(877..1131)
                     /locus_tag="AC161X_00155"
     CDS             complement(877..1131)
                     /locus_tag="AC161X_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010960311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR4987988.1"
                     /translation="MATNIYVGNLTFNTNSADLENLFAAHGTVSKAQVVTDRDTGRSR
                     GFGFVEMDSSDAAQKAIDALNGKDVDGRQLTVNLAKERSR"
     gene            complement(1351..>3494)
                     /locus_tag="AC161X_00160"
     CDS             complement(1351..>3494)
                     /locus_tag="AC161X_00160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4987989.1"
                     /translation="KYTHVFEEPDRDEGYEVEGFHWGGLTAGDWKTVFWILLAPFALA
                     NVAGWTSKSNSNWGVALVRVTALSLTALLVSQAIIALVLIPYLWFVKEFASLSSDTGN
                     LAAIGLFVVTILLFLLLVRTSTQSHFTTLTPRKQFSLLVTPRVGAMLPPEDETGMDTG
                     IVEDPWADPAGTVATDPAIWRPHAILHRLRRLHLAIGVTVIAMAAALWAHHLLLFWLG
                     VALFGILVLTTFATSFGPRHRAVLWVTAVSSVTSLILALISVGAIAFSDPPGLEMEGL
                     HNLTFAITVVLGVSAMACVFNAGWLTVGALVVASQFGAVLGIAVGLIAEQLADIKPVL
                     VAHGAGFVAVAMLFLIGVIVITALILSAIPHPRSSEKELTTLLRRIVLRGPWVFYAAA
                     AYGIGFGVLVLFQSGRESARAADVTGIAGIFSTNFGKAMFVGFTPKSLTVADSGSLVW
                     NIAIAMGALIVLLAWWRISATKGWKFGLLVPIGAAALVLLTGDDFKTSFLGLDFVLTR
                     QLVDIAVFVTVLIPGLFMLKSIISGVRQGEKSRRQVGILWDVGSFWPRWFHPLAPPGY
                     GPIAVKGLQKELKANHREILAAHSQGTLISAVALSQMDEADLPDSFVTYGSQLGVLFP
                     AMFPAADIDGLVAEVASRFQDRWINLWRDDDPIGGQFIETIALSNWQVCTGHGHSGHE
                     VTPEYRVARARVLEGVPTWPDDMPKPECWGQ"
BASE COUNT          705 a         1030 c         1136 g          623 t
ORIGIN      
        1 tgttcgaggg tcggatcgcc gaggaattag gacgcctaag tggtatcgga cagattcgcg
       61 tcatagacgc tgtggtcgtg acccgtgagg gcgacgacgt cgcggtgctt tcggtgtccg
      121 acctcgacga cgaggagcga gccgagttgc ggaccgtggt gggcgccctg atagggctag
      181 gcgttgcagg catcgatggt gccgttgctg gcgccgaggc gggagcatcc atcgacgtgg
      241 acgcactgac cgctgccgag atggtcggtg ccagcttgat cgaagggctt cccgacggca
      301 gctcggcgct cgtcctcgcg atcgagcatc tctgggcgat accgctccgt gatgcggtcc
      361 gcgacgcggg aggagtggtc atggcccatc gcagtctcac ggctgaggac ctgatcgcga
      421 tggggatggc catcggcatg gaagccgaga tggacatgga gaaagaagag ctcgccgaca
      481 gcgaggcctg attctgacgg atccgggccg agaagaaata catcgtggct ggtggaacgg
      541 ccccgtgtag ttgcttctcg gcgatgagcg ccctcgggag gactcgaacc tccgacacca
      601 ggtttaggaa acctgtgctc tatcccctga gctacgaggg cgagggcgag ggcgacccgg
      661 ctcaccgcca ttcaagggaa agaatagagg ccaggcccga atcggacctg gcctctgcga
      721 gttggttggg gctggtgcct taagaccggc cttcacactt gcatcgaggg accgtgcaca
      781 tcgcctggcg gtgctccttg tttgggtgcc cgcagccgtt gcagcgatcc gtcttcggtt
      841 tgtagtcgcc gcgctttggc ttggggaccg ctttgatcaa cgagagcgct ctttggcaag
      901 gttgacggtg agctggcgcc catcgacgtc cttgccgttg agagcatcga tcgccttctg
      961 ggcggcgtca gatgagtcca tctcaacgaa gccgaacccg cgcgaacggc cggtgtcacg
     1021 atcggtgacg acctgggcct tgcttacggt gccatgggcg gcgaagagat tctcgaggtc
     1081 ggctgagttc gtgttgaacg tcaggtttcc gacatagatg ttggtggcca ttgtgctgct
     1141 ccttcttgga agctcgaacc gtctgattgc tcaacgcccc acaggtctcc ttgcgagaac
     1201 ctcgttcttg gggagcgggg gtccggcttg gccctgcggc gtcttcaccc gacggaacga
     1261 cagaagcgaa gaaagggcgc agcacgcatt aggcgtgcgc tgtgcaaatg agtgtagtcc
     1321 ttcgttgtgg acaactcctg gtcggggcgg tcattgaccc caacactccg gtttgggcat
     1381 gtcgtcgggc caggtcggca caccttccaa gaccctggct cgagctactc gatactcagg
     1441 tgttacctca tggccagaat ggccgtgccc ggtgcacacc tgccaattcg agagcgcgat
     1501 ggtttcgatg aattgaccgc cgatcgggtc atcgtcacgc cacaggttga tccatctgtc
     1561 ctggaatctg ctcgctacct cggcaaccaa tccatcgatg tcggcggccg ggaacatcgc
     1621 cgggaacagg acgccgagtt gggatccgta ggtgacgaag gagtcgggga gatcggcttc
     1681 atccatctgg gacaacgcca cggcagagat gagagtccct tgggaatggg cagcaaggat
     1741 ctcgcggtga ttggccttca gttccttctg gaggcctttg actgcgattg gcccgtatcc
     1801 gggtggcgcc aggggatgaa accatctggg ccagaaggag cccacatccc acaaaatccc
     1861 aacctggcgc cgactcttct ctccctgtct gacgcctgag atgatgctct tgagcatgaa
     1921 cagtcccggg atcaacacag tgacgaagac ggcgatgtcc accagttggc gggtcaacac
     1981 gaagtcgagg ccaaggaacg acgtcttgaa gtcatctccc gtaaggagaa cgagtgcagc
     2041 tgctccgatc gggacgagaa gaccgaattt ccaccctttg gtcgcggaga tccgccacca
     2101 ggcgagcagc acgatcaggg cgcccatggc gatggcgatg ttccagacca aactgcctga
     2161 gtcagccaca gtcagagact tcggcgtgaa cccgacgaac attgccttgc cgaaattcgt
     2221 cgagaagatc ccggcgatgc ccgtcacgtc agccgcccgg gcgctctctc gaccggactg
     2281 gaagaggacc aggactccga atccgatccc gtaggccgcc gccgcataga acacccacgg
     2341 cccgcggagg actatgcgcc gcaacagcgt cgtcagttcc ttttcgctgc tccgggggtg
     2401 ggggatcgcg ctcaggatca acgcggtgat cacgattact cctatcagga acaacatcgc
     2461 caccgctacg aacccggcac cgtgtgcgac gaggaccggt ttgatgtcgg ccaattgctc
     2521 ggcgatgaga cccaccgcaa tcccgagcac cgccccgaac tgcgaggcaa cgaccagggc
     2581 tccaacggtc agccagccgg cattgaagac gcaggccatt gcggacacac cgagaaccac
     2641 ggtgatcgcg aaggtgaggt tgtgcaggcc ctccatttcg agtccgggcg gatcggagaa
     2701 ggcgatggcc cccacactga tcaaggcgag aatcagcgat gtgacagagg agacggcggt
     2761 cacccacaga actgctcggt gcctcgggcc gaaagaggtg gcaaacgtgg tcagcaccaa
     2821 tatcccgaag agcgcaacac ctaaccagaa cagcaggagg tgatgggccc agagggcggc
     2881 ggccatggcg atgaccgtga ctccgatggc gaggtgcagc cgtcgcagcc gatggaggat
     2941 ggcgtgtggc cgccagatcg cgggatcggt ggccacagtt cccgcgggat ccgcccacgg
     3001 atcctccacg atgcccgtgt ccatccccgt ctcgtcctcc gggggcagca tcgcgccgac
     3061 tctgggcgtc accaggagcg agaactgctt cctgggcgta agcgtcgtga agtgggattg
     3121 ggtggacgtt cgaaccagga ggagaaacag aaggatcgtc accacgaaca agccgatcgc
     3181 cgccaggttg ccggtgtccg agctcagcga ggcgaactct ttgacaaacc agaggtaggg
     3241 gataaggacc aaggcgatga tcgcctgcga aacgagcaac gccgtgagcg acagggcggt
     3301 gacccggacc agggcgacac cccaattgct attgctcttc gaggtccatc cggcgacgtt
     3361 ggcaagggcg aacggggcga gcaggatcca gaacacggtc ttccagtccc ctgcggtgag
     3421 gccaccccaa tggaaccctt cgacttcgta gccttcatcc cggtcaggct cctcaaagac
     3481 atgggtgtac ttgt
//
LOCUS       DBNHXU010000008         2420 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_200658, whole genome shotgun sequence.
ACCESSION   DBNHXU010000008 DBNHXU010000000
VERSION     DBNHXU010000008.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2420)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2420
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_200658"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..273)
                     /locus_tag="AC161X_00165"
     CDS             complement(<1..273)
                     /locus_tag="AC161X_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4987990.1"
                     /translation="MFDALEAPPDCIAPEDLIEELESLFTHRDRYDARIATVMQQIEE
                     TKAFSKDGYTSTTAMLKHRMSLHPGEAWRHVTRANGLAQAPLTTFAY"
     gene            complement(370..714)
                     /locus_tag="AC161X_00170"
     CDS             complement(370..714)
                     /locus_tag="AC161X_00170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HIR4987991.1"
                     /translation="MPIEASTQRIRVEIDGVTVADSERPMFLYERGLPVRYYLPPSDI
                     RMDLLVPTDTSTTCHWKGQARYWSAAIDGKSYKDIAWGYDHPLAEAEAIAGLVSFYNE
                     KVDVYVDEVLQP"
     gene            complement(723..1637)
                     /locus_tag="AC161X_00175"
     CDS             complement(723..1637)
                     /locus_tag="AC161X_00175"
                     /inference="COORDINATES: protein motif:HMM:NF017085.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HIR4987992.1"
                     /translation="MTGFYDGEEAFGEAVHAARKSTKKVRALLRLVRSEIGEKVYRFE
                     NRAMRDTARMLSSVRSAAVMGNALDDLELLYGSFLADGTFEETHERLEANRARVETRA
                     MEDPELVPRVVANLERAHSRYASWPTDSHAREVYGTGIRDRYSSIGPGLKATYRRGRS
                     EMVMAYRNPSPATFHSWRKRVKYLKHQMEIITPLWPEVVLGMVITLDRMSELLGEDHD
                     LAELLESLTDRPELCPNPMERSLLRALAEQRRSDVETASRILGRRIYAEGPAGLKGRF
                     GAYWESMELARDMALAMNGGESASLRSG"
     gene            1653..2228
                     /locus_tag="AC161X_00180"
     CDS             1653..2228
                     /locus_tag="AC161X_00180"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HIR4987993.1"
                     /translation="MKTEPLETDAQGLASMESEVRESQDDGIRMGRLGSQKGHPASVM
                     SGSVRSAGLLPFRQHDGIEVLVAHPGGPYFAHRDNGWWSLVKGMVEKGEHDEVAAARE
                     FEEETGWPAPSQPWIALGDVVMKSRKVVVAWAVEADYDIDTLVPGLFSMGHQRFPEID
                     RVQWMRPELARVKLNPAMGTFVDRLEQLLGT"
     gene            2284..>2420
                     /locus_tag="AC161X_00185"
     CDS             2284..>2420
                     /locus_tag="AC161X_00185"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011311617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase"
                     /protein_id="HIR4987994.1"
                     /translation="MSDTPQTEAEWRQKLDPEQYQVLREAGTERAFTGKYWNSKAHGT
                     YR"
BASE COUNT          479 a          749 c          734 g          458 t
ORIGIN      
        1 ataggcgaag gtggtcagcg gcgcttgggc cagtccattc gccctggtga catgtctcca
       61 ggcttccccg ggatgcaggg acatccgatg tttcaacatc gcggtcgtcg aggtgtaacc
      121 gtccttactg aacgcctttg tctcttcgat ctgctgcatc acggtcgcaa ttcgggcgtc
      181 gtagcgatcg cgatgggtga acagagattc cagctcttcg atcagatcct cgggggcgat
      241 gcagtcgggt ggtgcttcca gcgcgtcgaa catatgttcg taacgtactc aaccccggtg
      301 acagattcca taccctcgcg gccaagttcg aaaagaattc cttcggccgt cgaggaagca
      361 ccgccgaacc tagggctgaa gcacctcatc gacatagacg tccaccttct cgttgtagaa
      421 ggagaccagc ccggcgatgg cttcggcttc ggctagcggg tggtcatagc cccaagcgat
      481 gtccttgtac gacttgccgt cgatagcggc cgaccagtag cgagcctgac ccttccaatg
      541 acatgtggtg gaggtgtcgg tagggaccaa gagatccatg cgaatatcgc tcggaggcaa
      601 gtaataccgc accggaagac cgcgctcata gaggaacatg ggccgctccg agtcggcgac
      661 ggtgaccccg tcgatctcca cgcgaatccg ctgggttgaa gcctctattg gcacagccaa
      721 gatcaaccgc tccgaagaga ggcagattcc cctccgttca tggctaacgc catgtcgcgg
      781 gccagttcca tcgattccca gtaggcaccg aacctcccct tgagccctgc agggccttcc
      841 gcgtagatcc ggcgccccag gattcgggaa gcagtctcga cgtcagacct cctctgctcg
      901 gcgagagccc tcagcagaga gcgttccatc gggttggggc acaattcggg acggtcggtc
      961 agcgactcca acagctcggc caggtcgtgg tcctctccca agagctcgga catccgatcg
     1021 agggtgatca ccatccccag caccacttcg ggccacaacg gcgtgatgat ctccatttgg
     1081 tgtttgaggt acttgacccg cttcctccaa ctgtggaagg tggccgggct tggattccga
     1141 tacgccatga ccatctcgct cctgccgcgt cgataggtgg ccttcaaccc tggaccgatg
     1201 ctcgaatacc tgtcacgaat cccggtcccg tacacctctc tggcgtggga atcggtcggc
     1261 caggacgcat atcggctgtg agctcgttcg agattggcca ccacgcgggg caccagctcg
     1321 ggatcctcca tggccctggt ctccactcgg gcccgattcg cctcgagacg ctcgtgcgtc
     1381 tcttcgaaag ttccgtcggc aaggaatgac ccgtagagca gctcgagatc gtcgagcgcg
     1441 ttgcccatca ccgccgccga gcggacgctg ctcagcattc gcgcggtgtc acgcatggcc
     1501 cggttctcga agcggtagac cttttcgcca atctccgacc gaacaagccg gagcaaggcc
     1561 cgaaccttct tcgtcgactt ccgcgcagcg tgaaccgcct cgccaaaagc ctcttccccg
     1621 tcatagaagc cggtcaccgc gacctccatt tcatgaagac tgagccgttg gagaccgacg
     1681 cccaagggct cgccagcatg gagtcggaag tcagggagag tcaggacgac ggcatccgca
     1741 tggggaggct gggcagccaa aagggtcacc cggccagcgt aatgtcgggg tctgtgagat
     1801 ccgcgggtct tctccccttc cgacaacacg atggcatcga ggtgctggtc gcccaccccg
     1861 gtgggcccta tttcgcacat cgcgacaatg gctggtggtc gctcgtcaag ggcatggtgg
     1921 agaaagggga gcacgacgag gttgccgcgg cgcgggaatt cgaagaggag acgggctggc
     1981 ccgccccatc tcagccctgg atcgcgcttg gcgatgttgt catgaagtca cgcaaggtcg
     2041 tcgtcgcctg ggcggtcgag gccgactacg acatcgacac cctggtcccc ggcctgtttt
     2101 cgatgggcca ccagcgtttt cccgagatcg atcgagtgca atggatgcgg cccgagttgg
     2161 cccgggtcaa gctgaatccg gcgatgggga cgttcgtcga tcgtttggaa caactcctcg
     2221 gcacctgatg tttcgacatg tgagatctga gcgaagacgg atttacagaa acggagaacg
     2281 acaatgagtg acacgccgca gacagaagca gagtggcgcc agaagctcga ccccgagcag
     2341 taccaagtgt tgcgcgaggc cgggaccgag cgggcgttca ccggtaagta ctggaactca
     2401 aaggcccacg ggacctaccg
//
LOCUS       DBNHXU010000009         5970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_994377, whole genome shotgun sequence.
ACCESSION   DBNHXU010000009 DBNHXU010000000
VERSION     DBNHXU010000009.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5970
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_994377"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..479
                     /locus_tag="AC161X_00190"
     CDS             <1..479
                     /locus_tag="AC161X_00190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4987995.1"
                     /translation="FETPLTMGQANELLESDADVRVVWAGFEVGDGSGLSSTGVLGYG
                     TCGTPSIEVSGAIGASGGGSGSWSGEPASIDDALAETLRAVRNLIDHPELLDGIDVRA
                     EEAESVLVELGDPQVASLVVTGPTKEIVAFIEEAAPDVVNVLALDFTNWFQPLCGR"
     gene            484..1206
                     /locus_tag="AC161X_00195"
     CDS             484..1206
                     /locus_tag="AC161X_00195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4987996.1"
                     /translation="MTLYRDIRRNRRWVLIGVVLGYGMTLAVLVIRLVNTGTESGEVL
                     GSIALAASAAIGPSLAWLSLDRRPGLLPAAALASLITGIISIVLLPVAFVTSLIWMYA
                     WTSRPVKVEISRGQWWSGVALGMTAALAVLVLFVHLDPVCTETLADGTVVQVDPAEQG
                     YRSGWRLGSDTSVSSGTGSSGPDGPIASSCSSDTIVWGEALASLIVSLGAIAAAARWW
                     PLNARHSVEESLDVGTASRDLT"
     gene            1211..1960
                     /gene="dapB"
                     /locus_tag="AC161X_00200"
     CDS             1211..1960
                     /gene="dapB"
                     /locus_tag="AC161X_00200"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010841435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HIR4987997.1"
                     /translation="MIQVVISGAGGKLATAIADAVVAADDLELTGLFNPKRAGQTMYG
                     KTVSGDHDAMTGDVVVETAHNDVVLENLGHWQANGLAAVVGTSGFTPERLAQLRESWG
                     SDGPACLVVPNFAIGAVLMMRFAAQAAPYFEFVEIVERHSSTKPDAPSGTSLATAIGV
                     SAAGGRSAEGSEELVDGARGALVEGVRVHAMRLPGLISQQEVALSNTGEVLSIEHLST
                     EYGAFAAGALLAIRKVHDLEPGVHLGLDVVL"
     gene            complement(1989..2765)
                     /locus_tag="AC161X_00205"
     CDS             complement(1989..2765)
                     /locus_tag="AC161X_00205"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HIR4987998.1"
                     /translation="MLAVVIWGVIQGITEFLPVSSDGHLVVIPALLNEMGLDVQPPTL
                     ASTAILHLGTLVAMFVYFRHDLARVIRFRTDPEGRKIALLVAIGTIPAVIFGLPLEGE
                     LNAFSTTISNVGWALIGNGVILYLGSRLAFGTRRIMDMKIPDSILIGLAQAMALIPGI
                     SRSGTTISVGSARHIEPTEAARYSFLLGIPAVTGAGLLELYRVSGSGTFDPILIVGVI
                     VAGITGYLAIGWLMASLKRVGLLPFAIYCVALGALTVVVF"
     gene            complement(2782..3300)
                     /locus_tag="AC161X_00210"
     CDS             complement(2782..3300)
                     /locus_tag="AC161X_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4987999.1"
                     /translation="MLWLQTPPWGRWLAAGLIVLGSLWVELRPAPSVEHPFASEEILA
                     GAEIGDWNIVLRRVPAGLFEAVAPGGRALTNIAPGEPLLASTVGEHDQTTPAGWWSIE
                     MTLPISARPGDQAQIVLLDAGSTVPAVVVAAVADDPLGSGLGIVAVPGDQAADVAAAA
                     VEGRAAIMIATG"
     gene            complement(3349..3732)
                     /locus_tag="AC161X_00215"
     CDS             complement(3349..3732)
                     /locus_tag="AC161X_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016472568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HIR4988000.1"
                     /translation="MYDRDPPLSSGQISMPTYEYVCKSCGATVEKVQSFSDKPLRRHE
                     TCGGDLQKVFHARGIVFKGPGFYTTDARASSNTSKNSEGSKAKETSKASKDGSGSSSK
                     EPSKSSSKEPSKSSVDSPTPSSSSD"
     gene            3781..4386
                     /gene="plsY"
                     /locus_tag="AC161X_00220"
     CDS             3781..4386
                     /gene="plsY"
                     /locus_tag="AC161X_00220"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HIR4988001.1"
                     /translation="MTPGLAIPLALMAAYVVGSIDFAVIVARMHGVDIHEAGSGNPGT
                     SNVLRTVGRLPAAMVLIGDMLKGTVGAAMGMIASGVANPMGPWAFAAGFAAVVGHCYP
                     IFHKFKGGKGVATGAGVLLFTVPVAAVIELALWGIIVKLTKTASIASLVIMVVTIPIL
                     IWQGVPGMSLVWVALIIALIVWRHRGNIQRMGSGREHKVTT"
     gene            4383..5198
                     /locus_tag="AC161X_00225"
     CDS             4383..5198
                     /locus_tag="AC161X_00225"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: protein motif:HMM:NF012695.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003983 - UTP:glucose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase"
                     /protein_id="HIR4988002.1"
                     /translation="MNIDVAVIPAAGRGTRMRPATRVVPKALLTVVDRPAIQYAVEEA
                     ARAGAKEAVVIVDLDVGHLVAQHFSLEGPLPGLEEIHLRPVVQEEPLGLGHAVNEAAD
                     VVGDRPFFVLLADNIVRPGVDVLASLAAGSNDGTVSVVCLRELDDEFLSKYGVVVPSS
                     PVEESYLMLGGAVEKPGIEEAPSRLGMVGRYLFTPEVFGLLDKTAPGTGGEIQLTDAI
                     DGLGKAGRLRGFVAALDLLDVGTPLGLLEASVVLGKEQFGEEFTTWLEGRPGT"
     gene            5195..>5970
                     /locus_tag="AC161X_00230"
     CDS             5195..>5970
                     /locus_tag="AC161X_00230"
                     /EC_number="2.10.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF015418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin molybdotransferase MoeA"
                     /protein_id="HIR4988003.1"
                     /translation="MRPLEEARSEVLRSTEALESEAVSIWEASGRVLATDVTATESVP
                     PFHNSAMDGYAVLGADVARPGAVLDVLGDLAAGFVAESRVGPGQALKIMTGAPMPEGA
                     DTIVKVEDTDGGTDRVTVVPAVDTGTSVRAAGGDVALGSLVFEAGTRLSPMHVGVLAT
                     IGVAAPVVYRRPRVAYMSTGDELVPPGSGPLAPGMIRDSNRPMVKALLEDAGAELIDL
                     GIIPDNDEALREALKTGSSFDVIVSTGGVSMGDYDVTKKVL"
BASE COUNT         1043 a         1809 c         2024 g         1094 t
ORIGIN      
        1 gattcgagac acccctcacg atgggccagg caaacgaact gctcgagagc gacgccgatg
       61 tgcgcgtggt ctgggctgga ttcgaggttg gtgacggctc ggggctgtcg tccaccggtg
      121 tcctcgggta cgggacatgc ggtactccat cgatcgaggt ttcgggagcc ataggggcga
      181 gcggcggtgg cagtggtagc tggtcaggcg agccggcttc gatcgatgac gcactggctg
      241 aaaccctccg ggccgtgcgc aacttgatcg accacccgga gcttctcgac gggatcgacg
      301 tcagagcgga ggaagcagaa tcagtcctgg tcgagctggg cgacccacag gtggcttccc
      361 tggtggtcac cgggccgacc aaggagatcg ttgccttcat cgaggaggcc gcccctgacg
      421 tcgtcaatgt gctcgccctt gacttcacga actggttcca acccctctgt ggaaggtgac
      481 gcaatgaccc tgtatcgaga catccggaga aaccgacgct gggtgctgat cggagtcgtt
      541 ctcggatatg ggatgacact cgccgtcctt gtcatccgac tcgtcaacac cgggaccgag
      601 tctggggagg tcctggggtc gattgccctc gccgccagcg cagccattgg cccctcgttg
      661 gcctggctgt cgctcgatag gcgacccggt ttgctcccag ctgccgccct tgcctcgttg
      721 ataaccggga tcatcagcat cgtgctgctc cccgtcgcct tcgtgacttc gttgatctgg
      781 atgtatgcct ggacgagtcg accggtgaag gtcgagattt cgagagggca gtggtggtcc
      841 ggcgtggcgc tgggcatgac ggccgccctt gccgtcctcg tcctcttcgt ccacctcgac
      901 ccggtctgca cagagacgct ggccgatggc accgttgtgc aggtcgatcc ggcagagcag
      961 ggatatcggt ccggctggcg ccttgggtcc gacacgtcag tttcttcggg gaccgggagc
     1021 tcggggcccg atggcccgat cgcgagctcg tgcagctcgg acaccatcgt ttggggggaa
     1081 gccctggcgt cactgatagt cagcctcggt gccatcgccg ccgccgcccg atggtggccg
     1141 ctcaatgcca ggcactctgt tgaagaatct ctcgacgttg ggactgcttc acgcgaccta
     1201 acgtgattcc atgatccaag tggttatctc gggtgccgga ggaaagctgg cgaccgcaat
     1261 cgcagacgcc gtggtcgccg cggacgatct cgagttgacc ggcctcttca atccaaaacg
     1321 ggccggccag acaatgtacg gcaagacagt gagcggggat cacgacgcca tgaccggtga
     1381 tgtcgtcgtg gagaccgcac acaacgatgt cgtcttagaa aacctcggtc actggcaagc
     1441 caacggcctg gcggccgtgg tcgggacctc cggcttcacc ccggagcgcc tcgctcagct
     1501 tcgggagtcc tggggatcgg atggcccggc ctgtctggtc gttcccaact tcgcgatcgg
     1561 ggcggtcctg atgatgagat tcgccgcgca agcggcgccg tacttcgagt tcgtggagat
     1621 cgtggagagg cactcttcga ccaagcccga cgcaccctca ggcacctcac tggctaccgc
     1681 gatcggggtg tccgccgcag ggggccgttc cgccgaaggg tctgaggagc tggttgacgg
     1741 agctcgaggg gccttggtgg agggagtccg tgtccatgcg atgagacttc ccgggctcat
     1801 ctcccaacag gaggtggcct tgtccaacac gggtgaggtg ctttccatcg agcatctgtc
     1861 gaccgagtac ggggccttcg ccgctggtgc attgttggcg atccgcaagg tccatgacct
     1921 cgagccaggg gtacacctcg ggttggatgt ggttttgtag gacgccgggg ccgccgctcc
     1981 agggtcggct agaaaacgac gaccgtcagc gcacccaggg ccacgcagta gatggcaaag
     2041 ggaagcagcc cgaccctttt cagagatgcc atcagccagc cgatcgccag atacccggtg
     2101 atcccggcca cgatcacacc gacgatcagg atcggatcga aagtcccaga gcccgacacc
     2161 ctatagagct cgagcagtcc ggcgccggtg accgcaggaa tcccgagcag gaaggagtat
     2221 cgggcggcct ctgtcggctc gatgtggcgg gcactgccga ccgagatcgt tgtgcccgat
     2281 ctcgatatcc cggggatcaa ggccatcgcc tgggcgagtc cgatgaggat cgagtcgggg
     2341 atcttcatat ccatgatgcg acgggtgccg aaggccagcc gggagccgag atacaggatc
     2401 accccgtttc cgatcagagc ccatccgaca ttggaaatcg tcgtcgagaa cgcattcagc
     2461 tcgccttcga gaggcagtcc gaagatcacg gccgggatgg tcccgattgc caccagcaac
     2521 gcgatctttc gcccttcggg gtcggtccgg aagcggatga cccgcgccag atcgtggcgg
     2581 aagtagacga acatcgccac gagggtcccc agatggagga tggcggtcga agccaacgtg
     2641 ggaggttgca catcgagccc catctcgttg aggagggcag gtatcaccac caggtgacca
     2701 tccgaggaga ccgggaggaa ttcggtgatc ccctggatca ctccccatat gacgacggca
     2761 agcacggcgg cacggtaacc ctcacccggt cgcaatcatg atcgcggccc tgccctccac
     2821 cgcggccgcc gccacatcgg cggcctggtc tcccgggacc gcgactatgc cgagaccgga
     2881 gcccaaaggg tcgtcggcga ccgctgccac caccactgca ggcacggtgg accccgcatc
     2941 gaggaggacg atctgcgcct gatctccggg gcgtgccgag atgggcagtg tcatctcgat
     3001 gctccaccat ccggccggcg tcgtctgatc gtgctctccg accgtcgaag cgagaagagg
     3061 ctccccggga gcgatattgg tcaacgccct cccgccggga gccacggcct cgaacaatcc
     3121 cgccggcacc cgccgcagga cgatgttcca atcgccgatc tcagccccgg cgaggatctc
     3181 ttccgacgca aatggatgtt caactgatgg cgcgggtctg agttcgaccc acaaggatcc
     3241 gaggacgatg agacctgctg ctaaccagcg tccccatggc ggtgtctgca accagagcac
     3301 cctttgaagg tacgggtgcc ggctggccgc cggaagaggg acggctgttc agtcgctgga
     3361 ggaagaaggg gtgggcgagt ccacggacga cttcgagggc tcttttgaag aagacttgga
     3421 aggttccttg gaagaggacc cggaaccatc cttgctggcc ttggatgtct ctttggcctt
     3481 ggagccttcg ctgttcttcg aggtattcga agaggcgcga gcatcggtgg tgtagaagcc
     3541 ggggcccttg aagacgatcc cgcgcgcatg gaagaccttc tggaggtcgc cgccacaggt
     3601 ctcgtgtcgg cgcaggggct tgtccgagaa ggactggacc ttttcgaccg tggcgccgca
     3661 cgacttgcag acgtattcgt aggtaggcat cgaaatctga ccgctggaga ggggtgggtc
     3721 gcgatcgtac aaccgccaac cgttcacttc cattccgacc ccggcaatat gatcgccccg
     3781 atgacacccg gtcttgcgat ccctctggcc ctgatggcgg cctatgtcgt gggaagcatt
     3841 gacttcgcgg tgatagttgc ccggatgcat ggcgtcgaca tccacgaggc cgggagcggc
     3901 aacccgggga cctcgaatgt gctccgcacc gtcggccggc ttccggcagc gatggtgctg
     3961 atcggggaca tgctcaaagg cacggtcgga gcagcgatgg ggatgattgc gtcgggagtt
     4021 gccaacccga tggggccctg ggcgtttgcc gcaggcttcg cggctgtcgt cggccactgc
     4081 tatccgatct tccacaagtt caagggcggc aaaggggtgg ctaccggcgc cggtgttctc
     4141 ttgttcaccg tgccggtggc cgcggtgatc gagctggcac tttgggggat catcgtgaag
     4201 ctgaccaaga cggcgtcgat agcttctctg gtgatcatgg tggtaacgat cccgatactg
     4261 atctggcagg gggtgcccgg catgtcactg gtctgggtgg ccctgatcat tgccctgatc
     4321 gtctggaggc atcgggggaa catccaacgc atgggttccg gcagagagca caaggtgact
     4381 acatgaacat tgatgtcgca gtgatccctg cggccggacg ggggacccgg atgcgcccgg
     4441 cgaccagggt ggtgccaaag gcgctgctga cggtggtcga ccgcccggcg atccaatacg
     4501 cagtcgagga ggcggcgcgc gctggggcaa aggaggcggt ggtgatcgtc gacctcgacg
     4561 ttggccatct cgtcgcccag catttcagtc tcgaagggcc actccccggt ctagaggaga
     4621 ttcaccttcg ccccgtggtc caggaggagc cgttgggtct ggggcatgcg gtgaacgagg
     4681 cggccgacgt ggttggggac cgacccttct tcgtccttct cgcggacaac atcgtgcgtc
     4741 ccggtgtgga cgttctggcg tccctggcgg ctggaagcaa cgacggaacg gtgtcggtgg
     4801 tctgcctccg cgagctcgac gacgagtttc tctcgaaata cggcgtggtg gtgccctcat
     4861 ccccggttga ggagagctat ctcatgctcg ggggagcggt cgagaagccc gggatcgagg
     4921 aggcgccttc ccggctgggg atggtgggcc gctacctgtt caccccggaa gtcttcggtc
     4981 tcctcgacaa gacggcccct gggaccgggg gcgagatcca gctgaccgat gcgatcgacg
     5041 gattgggtaa ggccgggagg ttgcgaggtt tcgtcgcggc gctcgacctc ctcgacgtgg
     5101 gcactccgct cggcctcctc gaggcgtcgg tggtgctcgg aaaggagcaa ttcggcgagg
     5161 agttcacgac ctggctggag ggtcggcccg ggacgtgaga ccactcgagg aggcccgctc
     5221 cgaggtcttg cgctcgactg aggcactgga gagcgaagcg gtctcgatct gggaagcgtc
     5281 gggtcgggtc ctcgccacag acgtgaccgc cactgagagc gttcccccgt ttcacaattc
     5341 ggccatggac ggttacgcgg tgctcggcgc cgacgtggct aggccaggtg cggtgctcga
     5401 tgtcctcgga gatctcgccg cgggcttcgt cgcagagagc cgggtcggtc cgggccaggc
     5461 gttgaagatc atgaccggcg cacccatgcc cgaaggcgcg gacacgattg tgaaggtcga
     5521 ggacaccgac ggtggcacag atcgggtgac cgtcgtcccg gcggttgaca ctggaacatc
     5581 ggtgcgggcg gccggaggtg acgtggccct cggaagcctc gtgttcgaag cagggaccag
     5641 gctcagcccg atgcacgtcg gggtgttggc aaccattggc gtcgccgccc ccgtcgtcta
     5701 tcgccgcccc cgggtcgcct acatgtcgac tggtgacgag ctcgtgccgc ccggaagcgg
     5761 tcctctggct cctgggatga tccgcgactc caatcggccg atggtgaagg ctctgctcga
     5821 ggatgccgga gccgaactga tcgatctggg gatcatcccc gacaatgacg aagcactgcg
     5881 ggaggctctg aaaaccggtt cctccttcga tgtgatcgtg tctaccggtg gcgtctcgat
     5941 gggggactac gacgtcacca agaaggttct
//
LOCUS       DBNHXU010000010         4974 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1589773, whole genome shotgun sequence.
ACCESSION   DBNHXU010000010 DBNHXU010000000
VERSION     DBNHXU010000010.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4974)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4974)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4974
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1589773"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC161X_00235"
     CDS             <1..210
                     /locus_tag="AC161X_00235"
                     /inference="COORDINATES: protein motif:HMM:NF014669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015225 - biotin transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015878 - biotin transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HIR4988004.1"
                     /translation="PTMFTAFVLGSFIIYFFGVIGLMITFDMTLSEAIAAGVVPFVIG
                     DLIKATAAGLLLPGAWRLVGEYPRP"
     gene            complement(215..649)
                     /locus_tag="AC161X_00240"
     CDS             complement(215..649)
                     /locus_tag="AC161X_00240"
                     /EC_number="2.8.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010038468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030366 - molybdopterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HIR4988005.1"
                     /translation="MEQPGQRIRVSVGEAQIDAAGLLSEVGRPDSGATVLFLGTVRDH
                     SDHVENVTHLEYEVYAGVVEASIREIVAEASDKWPLLSVAVEHRSGVVTLGEASVAVA
                     VATGHRADAFEAARFIIDELKTRAPIWKKEHWPGGAEWSQGS"
     gene            664..1824
                     /locus_tag="AC161X_00245"
     CDS             664..1824
                     /locus_tag="AC161X_00245"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR4988006.1"
                     /translation="MTIQGPEGPEPAIPIEEPAEPAQVGRRRLDPWTLVLGILGGALL
                     GTGVTFAILGSTGVFEEPTTTTNPPPPSVTTPPPTSTPPPVAVRASATDIAQHVIPSI
                     VAVEVAGLLGQSGGSGVVYGSDGYLITNDHVVEGAEEVTVVFADGARYPAQIIGTDPL
                     TDLAVLRVTREDLTPMDIGDPSALTVGDMAVAIGNPLALMGGPSVTSGIISAVDRWLE
                     VEVGTFLYGLVQTDAPITHGSSGGALVDENARVVGITTAIAVSDVAAEGFGFAIPIDM
                     ATDVADDLIESGTVNHALLGINGGTYFAEESEAQYPVGVLVHEVTAGDAYEAAGGQVN
                     DVIVALDGVPIDTLERLLTELRMHRAGDETSVGITRSDGDMNLTVTLGKYDP"
     gene            1821..2891
                     /locus_tag="AC161X_00250"
     CDS             1821..2891
                     /locus_tag="AC161X_00250"
                     /inference="COORDINATES: protein motif:HMM:TIGR03624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR4988007.1"
                     /translation="MSDDLFSQLFDLFDQPGPVNWKLAAEVARHLSGEQQAIEPWAAE
                     EYRELARLAEYRVEMIAPFRIIPASDVFPTDPRGWAEKNLETYGPLVEPFASSVVAEG
                     PVAPLMAQMTPAIVGLQVGSLVGSLSSWIVASFDAGLPQDRPGHLAVIVPNIETLTRS
                     AGLDRKEVRLWVVANEVAFRAVSQLPWIQDHLDHLLTNYADAVRIDPTQLSGLMAGGT
                     DPAAVEEAIARAGGIESILGGEEAEGPKRELEAFLGVMTGCARLLARRALGELSPGFD
                     RITAERDLARSEAEGRDLIGVGPVPPELTRLGDEFNQEVERRYGEDALEILWSDPTRM
                     PSADELQDPTSWAARVLLDGWS"
     gene            complement(2910..3626)
                     /locus_tag="AC161X_00255"
     CDS             complement(2910..3626)
                     /locus_tag="AC161X_00255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HIR4988008.1"
                     /translation="MGEAFLWGLLAASSLVLGALVVFVHAPEKRSLGLIMGFGAGVLL
                     SAVSFELIDEALTVGGGQGGTILGFFSGAIVFYVGDSLIARIGKGAEGEGSSGLSIVL
                     GAVLDGIPESAVLGLTLLQTGSIGVSMLVAVFISNVPEAIGASSDLLESGWKKSQIIG
                     LWSGVALVSAMAAAIGYLLLADAPPQTLAFVLAFAAGSILTMLSTTMIPEAFKDARRP
                     VGLATTLGFAVALTVSWIAG"
     gene            complement(3619..4566)
                     /locus_tag="AC161X_00260"
     CDS             complement(3619..4566)
                     /locus_tag="AC161X_00260"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhjD/YihY/BrkB family envelope integrity
                     protein"
                     /protein_id="HIR4988009.1"
                     /translation="MADPPDEEQTTAAGEQAEVTPGRIARARVRADEAKRVGAEMLAR
                     QEAHRVSVRVAVETYNRDRRFAGGLLAGGLAFRLFLWLLPFSLLVVALLGNVSTRLEQ
                     PPSDLAERAGLSAALAGTVAEAVHASGDGRWYLILVGAFFTLWAGMGVVKAFRLISGL
                     AWDVPPKMNFNPLVSSAMVCVVVAAVLAGHLVSAWIQRGPVASDVLALLGEAAFLVAV
                     GTWVFWKLPHAAEASWLAMVPGGVVVTVGVLVTRLVTAVYFAGRLDRVDDLYGALGVA
                     SVFLAWLFIIARLWVAGSSLNASTYIASEMNSPTATRDG"
     gene            4650..>4974
                     /locus_tag="AC161X_00265"
     CDS             4650..>4974
                     /locus_tag="AC161X_00265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988010.1"
                     /translation="MTAPEEATPRNPDTGMPATRRAAILTARLGVIQATLLLVSFWLL
                     SRIPGPTSPDAEFVAFYESDKRQLVVLVGLYLMPFAGIAFLWFAVALRMWLSRMTSTQ
                     DVLLSN"
BASE COUNT          971 a         1656 c         1485 g          862 t
ORIGIN      
        1 cccaccatgt tcaccgcgtt cgtcctcggc tcgttcatca tctatttctt cggagtgatc
       61 ggcctgatga tcaccttcga tatgaccctc agcgaggcga tcgctgcagg agttgtcccc
      121 ttcgtgattg gtgacctgat caaggccacc gccgccggcc tcctccttcc cggcgcttgg
      181 cgattggtcg gcgagtaccc gaggccgtaa agccctaaga gccctggctc cactcggcgc
      241 cgccaggcca gtgctctttc ttccagatcg gggcgcgggt tttcaattcg tcgatgatga
      301 aacgggccgc ctcgaatgca tctgcacggt gcccggtggc gaccgcgacg gccacagacg
      361 cctcgcccaa tgtgaccact ccggaccggt gctcgacagc gaccgagagc agaggccatt
      421 tgtcactggc ttcggccacg atctctctga tcgaagcctc gaccaccccg gcgtagacct
      481 cgtactcgag atgagtcaca ttctcgacat gatccgagtg atctcgcacc gtccccagga
      541 agaggaccgt cgcacccgaa tccgggcgac cgacctcgct cagaaggccg gcggcgtcga
      601 tttgtgcctc cccgaccgac accctgatcc gttgtccggg ttgctccata caatcccaag
      661 cctatgacga tccagggacc cgaagggcct gagccagcaa tcccgatcga ggagcctgcc
      721 gaacccgcgc aggtgggcag gcgacgcctc gatccgtgga ccctcgtcct cgggatcctc
      781 gggggtgcgc tactgggaac cggcgtcaca tttgccatcc tcgggtccac tggcgtcttc
      841 gaggaaccga cgaccaccac caatcctccc ccaccctcgg tgaccacccc gcctcccacc
      901 agcactccgc ccccggtggc ggtcagagcc tcggccacag acatcgccca acatgtcatc
      961 ccttcgatcg tcgctgtcga ggtcgccggt ctgctcgggc agagcggggg tagcggggtg
     1021 gtctacggct cggacgggta cttgataaca aacgatcatg tcgtcgaagg cgctgaagaa
     1081 gtgaccgtgg tcttcgcgga cggagccaga tacccggcgc agatcatcgg cacggacccg
     1141 ctgacagacc tcgcagtgct ccgggtgacc agggaagatc tcaccccgat ggacatcggt
     1201 gatccgtcgg cattgaccgt tggagatatg gccgtggcta tagggaaccc gttggcactg
     1261 atgggggggc cgtccgtgac atcgggaatc attagcgcag tcgaccggtg gctcgaggtc
     1321 gaagtcggca ctttcctcta tgggctggtc caaaccgatg ctccgatcac ccatggatcc
     1381 tcgggtgggg ccctcgtcga cgaaaacgct cgagtggtcg gtatcaccac tgccatcgcg
     1441 gtttcggacg tggctgccga aggcttcggc ttcgcgatcc cgattgacat ggcaaccgac
     1501 gttgccgacg atctcatcga gtcgggcacg gtgaaccacg ccctcctcgg gatcaacggc
     1561 ggcacctatt tcgcagagga gtccgaggct caatacccgg tgggggtcct cgtccacgaa
     1621 gtgaccgccg gcgacgccta cgaggcagct gggggccagg tcaacgacgt gatcgttgcg
     1681 ctcgacggag ttccgatcga cactctggag cgcctgctga ccgagttgcg aatgcaccgc
     1741 gctggagacg agacgtccgt gggtatcacg agatccgatg gcgacatgaa cctgaccgtg
     1801 actctgggaa aatacgaccc atgagcgacg acctgttctc tcaactcttc gatctcttcg
     1861 atcaaccggg gccggtcaac tggaagctcg ccgccgaggt ggcgcgtcat ctgtcaggtg
     1921 agcagcaggc aatcgagcct tgggctgcgg aggaataccg cgagcttgcc cggctcgctg
     1981 agtatcgggt ggaaatgatt gccccgttcc gcatcatccc ggcgtctgac gtctttccga
     2041 ccgatccccg gggctgggcc gagaagaatc tcgagaccta cggtccgctg gtcgagccct
     2101 ttgcctcctc ggtcgtggcc gagggaccgg tcgcacccct gatggcccag atgacgccgg
     2161 caattgtcgg ccttcaagtc ggctcgctcg ttggttcgct ctcctcttgg atcgtcgctt
     2221 cgttcgatgc cggcctgcct caggaccgac ctggccacct cgcagtgatc gtccccaaca
     2281 tcgagaccct gacgcgatca gctggtctgg accggaaaga ggttcggctt tgggtcgtag
     2341 ccaatgaagt tgcatttcgg gcggtttccc aactgccctg gatccaggat catctcgacc
     2401 acctcctcac caattacgcc gacgcggtcc gcatcgatcc gacccaactc agcgggctga
     2461 tggccggcgg taccgacccg gcagccgtgg aggaggcaat cgcccgcgcc gggggcatcg
     2521 agagcatcct cggtggcgag gaagccgaag gcccgaagcg agagctcgaa gcatttcttg
     2581 gtgtcatgac cggctgcgca aggctcctgg cgcggcgcgc tctcggcgag ctctcgcccg
     2641 gtttcgaccg gatcaccgcg gaacgcgacc tggcgaggtc ggaggccgag ggtcgagatc
     2701 tgatcggagt cggcccggtt cccccagagc tgacacgcct gggtgacgag ttcaaccagg
     2761 aggtcgaacg ccgctacggc gaggacgcat tggagatcct gtggtcagac ccgacgcgta
     2821 tgcccagcgc cgatgagcta caggatccga cttcgtgggc tgcgcgggtc ctcctcgatg
     2881 gatggtcctg atttcgaatt gtcgactggc tagccagcga tccaactcac agtcagagca
     2941 acagcaaagc cgagcgtcgt cgccagaccc accggccgcc gcgcatcttt gaaggcctcg
     3001 ggaatcatcg tggtcgacaa catggtgagg atcgaacccg cggcgaaggc aaggacgaag
     3061 gcgagagtct gcggcggagc atcggcgagc aacaggtacc caatggcagc ggccatcgcg
     3121 ctcaccagtg ccaccccgct ccacaaaccg atgatctggg atttcttcca ccccgactcg
     3181 agaaggtcgc tcgacgcccc tatcgcttca ggcacgttgg aaatgaagac cgccaccagc
     3241 atcgagaccc caatcgaacc cgtctgcaag agcgtgagcc cgagaaccgc cgactccggg
     3301 ataccgtcga gaacggcacc gagaacgatc gacagtcccg atgacccttc accttcagca
     3361 ccctttccga tcctggcgat cagcgaatcc cccacgtaga agacaatggc gccactgaaa
     3421 aacccgagga tggtcccgcc ctggcctcca ccgaccgtaa gggcctcgtc tatcaactcg
     3481 aatgacaccg cgctgagcag cactcctgcg ccgaacccca tgatcaaacc aagtgatctc
     3541 ttctccggtg catggacaaa gacgaccaaa gcgccaagaa cgagtgagct tgcggccaac
     3601 agcccccaca ggaatgcctc acccatcgcg tgtcgctgtc ggtgaattca tctccgaggc
     3661 aatgtatgtc gatgcgttga ggctggaccc ggccacccag aggcgagcga tgatgaatag
     3721 ccacgccaga aacaccgaag ccaccccgag agcgccgtag agatcatcca cccggtcgag
     3781 ccggcctgcg aaatagaccg ccgtaaccag gcgagtcacg aggactccga cggtgaccac
     3841 aacgccaccg ggcaccatgg ccagccagct tgcctcggcg gcatgaggga gcttccagaa
     3901 cacccaggtc ccaaccgcga ccaggaaggc ggcttcccct aagagagcca agacgtcgct
     3961 ggcaactggc ccccgttgta tccaggcgga taccaagtgc cctgcgagaa ccgcggccac
     4021 gacgacgcag accattgcgg aggaaacaag tgggttgaag ttcatcttgg gtgggacgtc
     4081 ccaggccaat cccgagatca ggcgaaacgc cttcaccacc cccatccctg cccagagtgt
     4141 gaagaacgca cccaccagta tcaggtacca gcggccatca cctgaggcgt gaactgcctc
     4201 ggcaactgtt ccggccaatg ctgcagagag gccggcgcgc tcggcgagat ccgaaggcgg
     4261 ttgctccaac ctggtggaca cgttgcccag aagcgctacg acgagcagcg agaacgggag
     4321 cagccagagg aacagtcgaa aagcgaggcc ccccgccaac aagccgcctg cgaaccgccg
     4381 gtcacgattg taggtctcca ccgcaacccg caccgaaacc cgatgggcct cctgccgggc
     4441 cagcatctcg gccccgaccc gcttggcttc atccgccctg actctcgctc gggcgatccg
     4501 accgggagtc acttccgcct gctccccggc tgctgtcgtc tgctcctcgt caggagggtc
     4561 tgccataccc gcaagctaga ccgggcgctg gtgctcaagg gcactcaacg ggcgcggtca
     4621 tcatcgggct aacttcgcct gcaagtgata tgacagcacc agaagaggcc acaccgagga
     4681 atccggacac ggggatgccc gccacccggc gcgcggccat cctcaccgct cgcttagggg
     4741 tgatccaggc gacacttctg ctggtctcgt tctggctgct cagtcggata ccgggcccaa
     4801 catcgccgga tgccgaattc gtcgcgtttt atgagtccga caaacgacag ttggtggtct
     4861 tggttgggtt gtacctaatg ccgtttgcag ggatcgcctt cctttggttc gcggtggcac
     4921 tgcggatgtg gctctcccgc atgacatcga ctcaggatgt cctgctttcc aacg
//
LOCUS       DBNHXU010000011         5778 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2383821, whole genome shotgun sequence.
ACCESSION   DBNHXU010000011 DBNHXU010000000
VERSION     DBNHXU010000011.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5778)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5778)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5778
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2383821"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..437)
                     /locus_tag="AC161X_00270"
     CDS             complement(<1..437)
                     /locus_tag="AC161X_00270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HIR4988011.1"
                     /translation="MSWEPVIGIEVHVELLTDSKMFCGCEVGFGAEPNTNVCPTCLGL
                     PGALPVTNRVAIESIMAIGLALNCEVSARSVFHRKNYFYADLPKNYQISQFDIPVCVG
                     GHLDIDVDGASHRVGIERAHMEEDTGKSTHVGAGGRIHAAESTL"
     gene            complement(434..1900)
                     /gene="gatA"
                     /locus_tag="AC161X_00275"
     CDS             complement(434..1900)
                     /gene="gatA"
                     /locus_tag="AC161X_00275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HIR4988012.1"
                     /translation="MTVNPGVAESGRMLRSGEATAVGLLEHVLERAGSSEAQLHAFLT
                     IDRERAFEAAAQADADLVDGIDRGPLHGIPVAIKDNMCTRGVETTAASQILAGYRPPY
                     DATVVSRLRDGGAVVVGKTNLDEFAMGSSTENSAYGPTYNPWDTNRVAGGSSGGSAAA
                     VAIGSALAALGSDTGGSIRQPASLCGVVGMKPTYGTVSRYGLIAFASSLDQIGPLTRT
                     VEDSAITLEAIWGHDPMDGTSYSGPYPDPKAGLGRGAEGLRVGVVKELGGEGYEPAVE
                     AAVAGMVSQLEGSGVEVVEVSLPSFEIALSAYYLIAPAECSANLARFDGIRYGHRGAG
                     KTTESLMARSRAEGFGPEVTRRILLGTYALSAGYYDAFYGQAQRVRAAIQDDFARAYQ
                     HVDVLVSPTSPTVAFEAGARTQNPLAMYLSDICTIPSNLAGHPAISVPVALDGQGLPI
                     GFQVMAPPLGEEVMYRVAAEVERLAGFEVAPGLVEVAS"
     gene            complement(1897..2187)
                     /gene="gatC"
                     /locus_tag="AC161X_00280"
     CDS             complement(1897..2187)
                     /gene="gatC"
                     /locus_tag="AC161X_00280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015746765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0006450 - regulation of translational
                     fidelity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HIR4988013.1"
                     /translation="MPVEIDIAHVARLARLDLSDEELEAYKSQLSVILDHAARVQSLD
                     ADPNPENAHPLGLTNIFRDDVVLPSLDRDEVLASAPETADGYFVVPPAMEGE"
     gene            complement(2198..3223)
                     /gene="dapE"
                     /locus_tag="AC161X_00285"
     CDS             complement(2198..3223)
                     /gene="dapE"
                     /locus_tag="AC161X_00285"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HIR4988014.1"
                     /translation="MTDLVETLSWLIDIPSVIGDEARIRDAIAGRLSGMPAQVVGDSL
                     VVGQPREQAVLLVGHLDTVPLQGHVGATVDGGRVHGLGATDMKGGLAVMIHLIESLGP
                     EHVACVFYAGEEGPLSGNQIGIILEAAPWLLGAAAAVVMEPTDRGLEAGCQGVINAEV
                     VFEGEAAHSARPWLGDNAITKGGEFLTRMRSLEPEPHIVGGLEFREVISVTTAHGGVA
                     KNVIPSLFTLNVNYRFAPDRSMDEALAHLREMCTDADRFSVADSAPAGAVSIDHPLFR
                     RLIEVSGSQVAAKQGWTDVAQLSSVGIPAVNFGPGEPALAHKPGESVRVDDLTWAYDS
                     LAAVLSS"
     gene            complement(3220..4026)
                     /locus_tag="AC161X_00290"
     CDS             complement(3220..4026)
                     /locus_tag="AC161X_00290"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012235582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HIR4988015.1"
                     /translation="MSDQELIRSAFAGDVDIEDARDAVERALDSLDRGEARVAEKTGG
                     RWVVNEWLKEAILLFFRLREMETIEVGGFEYHDKIPLKTGLAKQGVRVVPPGTIRYGA
                     YCEPGVIVMPGFVNIGAYVGAGTMVDTWATVGSCAQIGKGVHLSGGVGIGGVLEPPSA
                     SPVIIEDGAFIGSRSIVVEGVLVEEGSVLGANTVLTASTPIIDVTGPEPVEHRGRVPA
                     GSVVVPGTRPKEFPSGTYDLVCALIIGRRTEATDRKTALNDYLRELGVQL"
     gene            4092..4481
                     /locus_tag="AC161X_00295"
     CDS             4092..4481
                     /locus_tag="AC161X_00295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988016.1"
                     /translation="MSYRYIYLGLGLVAVAAIAFGIAFAIEGDAIELPGPVESVSPED
                     GSLNPPQTTVIIDLEVGYDAQIFVDDWLVTDATFVEGTGVYRWAPSASNPTINEWTPG
                     EHTIRVVYDTVNGLPDPGDFSWSFRVG"
     gene            4546..5460
                     /locus_tag="AC161X_00300"
     CDS             4546..5460
                     /locus_tag="AC161X_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018485052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR4988017.1"
                     /translation="MTELVGNTPVVKLAKVVPEGAADIFVKLEWFNPTGSYKDRMALA
                     IIEEAERRGDLKPGMTVVEYTGGSTGSSLAFVCAVKGYRFKVVSSDAFAPEKLRTMKA
                     FGAELTIVPSEGGMITPDLIPRMIEKATEYASEPGTYFTNQFHNRDALVGHRHFGEEL
                     LGQIGRPIDVFCTAVGTAGFSMGVSSVLKEASPSTRVVLFEPESTAVISGNAPGTHHV
                     EGIGVGFVPPLLDQGLYDEIRTIDEAEARVMARRLAAEEGLLTGTSSGLNVFGAIGIA
                     QELGAGHTVATVAVDTGLKYLTGNLFDD"
     gene            complement(5471..>5778)
                     /locus_tag="AC161X_00305"
     CDS             complement(5471..>5778)
                     /locus_tag="AC161X_00305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007462855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR4988018.1"
                     /translation="RGRKTGKLRRTALIFGVDGDRYVLVASVGGAAKNPAWYLNLVEN
                     PDVELQVKAEKFKGRARTASAEEKTRLWQLMAAIWPDYDSYQTKTEREIPVVIVERV"
BASE COUNT         1084 a         1858 c         1837 g          999 t
ORIGIN      
        1 agggtggact ccgcggcgtg gatcctcccc ccggcgccga catgggtcga cttgccggtg
       61 tcctcctcca tgtgggcgcg ctcgattccc actcgatgac tcgcaccatc gacatcgatg
      121 tcgaggtgtc cgccgacgca caccgggatg tcgaactggg agatctgata gttcttgggg
      181 agatcggcat agaagtagtt cttccgatgg aagacggacc tcgcggagac ctcgcaattc
      241 aaggcgaggc cgatggccat gatcgactcg atggcgacac gattggtcac cgggagcgcg
      301 ccgggaagac cgaggcacgt gggacagaca ttggtgttcg gttcggcgcc gaaaccgacc
      361 tcgcatccgc agaacatctt ggagtcggtg agaagctcga cgtgaacttc gatcccgatc
      421 acaggctccc aactcatgag gcgacctcca ccagaccggg tgccacctcg aagccggcca
      481 agcgctcgac ttcggcggca acccggtaca tgacctcttc gccgagaggc ggggccatca
      541 cctggaatcc aatcggcagg ccctggccat ccagtgcaac gggcaccgag atcgccggat
      601 gtccggccag attggagggg atggtgcaga tgtcggagag atacatggcg aggggattct
      661 gcgtgcgagc accggcctcg aaggccacgg tggggctcgt tggcgagacc agcacatcga
      721 catgctgata ggccctggcg aagtcgtcct ggatggcggc acgaacgcgt tgcgcctgcc
      781 catagaaggc gtcgtagtac ccggcggaga gggcgtaggt gccgagcagg atccgtctgg
      841 tcacctcggg gccgaacccc tccgccctgc tccgcgccat gaggctctct gtcgtcttcc
      901 ccgccccccg gtgcccgtag cgaatcccgt cgaatctggc gaggtttgcc gagcactctg
      961 cgggagcgat gaggtaatag gcggagaggg caatctcgaa ggaaggcagc gacacctcga
     1021 ccacctccac accggagccc tcgagctggg acaccatgcc cgcaacggct gcctcgacgg
     1081 ccggctcgta gccctcccct cccaattcct tgaccacacc cacccggaga ccttcggccc
     1141 cccgacccaa accggccttt ggatcgggat aagggcccga gtacgaggtg ccgtccatgg
     1201 ggtcgtggcc ccagatggcc tcgagggtga tggcggagtc ctccacggtt cgggtgagtg
     1261 gcccgatctg gtccagagag gatgcgaagg cgatgagccc atatcggctc accgtcccgt
     1321 atgtgggctt catccccacc accccacaca gggaggctgg ctggcgaatc gacccgccgg
     1381 tgtcggaccc gagcgcggcc agcgccgagc cgatggctac agcggcagcc gaccctcctg
     1441 acgagccgcc ggcgacacgg ttggtgtccc aggggttgta ggtaggccca tatgccgagt
     1501 tctcggtcga ggagcccatc gcgaactcgt cgagattggt cttgcccacg acgacggctc
     1561 ctccgtcccg gagccgggat accactgtgg cgtcgtaggg gggccgatac ccggcgagga
     1621 tctgggaagc ggccgtggtc tcgacgcctc tggtgcacat gttgtccttg atggcaaccg
     1681 ggatcccatg caaagggcca cggtcgatgc cgtcgaccag gtcggcatcg gcctgagccg
     1741 cggcctcgaa agcgcgttcc cggtcgatgg tgagaaaggc gtggagctgg gcctcggagc
     1801 taccggcgcg ctcgaggaca tgctccaaca gcccgaccgc ggtggcctcg ccggatcgga
     1861 gcatgcgacc cgactcggcc acacccgggt tgacggtcac tctccctcca tcgcgggcgg
     1921 gaccacgaag tacccatcgg cagtctccgg cgcgctcgcc agcacctcat cacggtccag
     1981 cgagggaagc accacatcgt cgcggaagat gttggtgagc ccgaggggat gagcgttctc
     2041 ggggttgggg tcggcgtcga gggattgcac tcgggcggcg tgatccagaa tcacgctcag
     2101 ctggctctta tacgcctcga gctcctcgtc actcaggtcg agacgggcca aacgggcgac
     2161 atgggcgata tcgatttcga ccggcatggc ccggagttta ggaagagagc accgccgcca
     2221 acgagtcgta tgcccaggtg aggtcgtcga cccgcaccga ctcccctggc ttgtgagcca
     2281 gtgccggctc gccggggccg aagttgactg ccgggatccc gaccgacgag agttgggcga
     2341 catcggtcca gccttgcttg gcggcaacct gcgaacccga tacttcgatc aagcggcgga
     2401 acaacgggtg gtcgatgctc actgcccccg caggagcgga atcggcaacc gagaagcgat
     2461 ccgcatcggt gcacatctca cgaagatgag ccagtgcctc gtccattgac cgatccggtg
     2521 cgaatcggta gttgacgttc agcgtgaaca gtgaagggat gacgttcttg gccacgccac
     2581 cgtgggctgt ggtgacggag atcacttctc tgaactcgag accgccgacg atgtgcggct
     2641 ccggctccaa tgaccgcatt cgagtcagaa actcgccgcc cttagtgatg gcgttgtctc
     2701 ccagccatgg ccgggcggaa tgggctgcct cgccctcgaa gaccacctcg gcgttgatca
     2761 caccctggca acccgcttcg agaccccgat cggtgggctc catgaccacc gccgcagccg
     2821 ctccgagcag ccacggtgca gcttcgagga tgatgccgat ctggttcccc gacaacgggc
     2881 cttcctcacc ggcatagaag acacaagcga catgctcggg cccgagggac tcgatgagat
     2941 ggatcatcac ggcgagacca cccttcatgt cagtggcccc gagcccgtgt actcgcccgc
     3001 catcgacggt ggctcccacg tgaccctgta ggggcacggt gtcgagatgc ccgacgagaa
     3061 gtacggcctg ctcccgaggc tggccgacaa cgagtgagtc gccgacgact tgcgcgggca
     3121 tcccggaaag gcgtcccgcg atcgcatcgc ggatccgggc ctcatccccg atgaccgagg
     3181 gaatgtcgat caaccaggaa agggtctcga cgagatcggt cacagctgga cgccgagttc
     3241 cctcaggtag tcgttgagcg cggtcttgcg atcggtggcc tcggtccttc gcccgatgat
     3301 caaggcgcag accaggtcat aggttccact gggaaactcc ttggggcggg tgccgggaac
     3361 gaccaccgaa cctgcgggga ctctcccccg atgttcgacc ggctcgggcc cggtgacgtc
     3421 gatgatcggc gtcgaggcag tgagcaccgt atttgcacca aggaccgagc cttcctcgac
     3481 gagcacgcct tcgaccacga tcgagcggct gccgatgaac gccccatcct cgatgatcac
     3541 cgggctcgca ctcggcggtt cgaggactcc cccgatcccc actccaccgg agaggtgcac
     3601 ccctttcccg atctgggcgc aagagccaac cgtggcccag gtgtcgacca tggtgccggc
     3661 gccgacatag gcgccgatgt tgacgaaacc cggcatgacg atcacaccgg gctcacaata
     3721 ggctccatag cggatggtcc ccggaggtac caccctgacg ccctgcttcg ccaaacctgt
     3781 cttcagcggg atcttgtcgt ggtattcgaa gcctccgacc tcgatggtct ccatctcccg
     3841 cagacggaaa aagaggagaa tcgcctcctt cagccactca ttgaccaccc agcgtccgcc
     3901 ggtcttctcc gcgacccggg cctcgcctcg gtcgagcgaa tcgagagctc gctccacggc
     3961 gtcacgtgca tcctcgatat cgacgtcgcc ggcgaaagcc gacctgatca gttcctggtc
     4021 cgacaaacct cgatcacctc acaacgtgtc ccggtggcag agtaccggtg cctggctacg
     4081 gtgcccgtgg catgtcgtac cgctacatct atctcggtct gggcttggtg gcggtagccg
     4141 cgatcgcgtt cgggatcgcg ttcgccatcg agggcgatgc catagagttg ccggggccgg
     4201 tcgagtcggt ctcccccgag gacggcagcc tgaatcctcc ccagaccacc gtgatcatcg
     4261 acctcgaagt tggctatgac gcccagatct tcgtcgacga ctggctggtc acagatgcca
     4321 cgtttgtcga gggaaccggc gtctacaggt gggccccgag tgcctccaac ccgaccatca
     4381 acgagtggac ccctggggag cacacgatcc gggtggtcta tgacacagtc aatggattgc
     4441 ccgaccccgg cgacttctcc tggtcgtttc gcgtgggttg aacgtccagg cataatcgcg
     4501 cgctgaccaa ccgaggagcc aaccatcaca cgactgggtt catccatgac cgaacttgtg
     4561 ggtaacaccc cggtggtgaa gctggccaaa gtggtgccgg aaggcgccgc cgacatcttc
     4621 gtcaagctgg agtggttcaa cccgaccggg tcctacaagg accgcatggc cctcgccatc
     4681 atcgaggagg ccgaaaggcg gggcgatctg aaaccgggga tgacagtcgt cgaatacacg
     4741 gggggaagca ccgggtcgtc tctggccttt gtttgtgccg tcaagggata ccgcttcaaa
     4801 gtggtgtcct cggatgcctt tgcccccgag aagttgagga ccatgaaagc gttcggcgcc
     4861 gagctgacga tcgtccccag cgaaggtgga atgatcacgc cggatctgat accacgaatg
     4921 atcgagaagg ccacggagta cgcctcagaa ccgggcacct acttcaccaa tcagttccac
     4981 aatcgtgacg cacttgtcgg acatcgacat tttggcgaag agttgttggg ccagatcggc
     5041 cggccgatcg atgtcttctg caccgcggtc gggactgctg gcttttcgat gggggtctcc
     5101 agcgtgttga aggaggccag tccttcgacc cgagtggtcc tattcgagcc ggagtcgacc
     5161 gcggtgatct cgggaaacgc gccgggcacg caccatgtcg aagggatcgg agtcgggttc
     5221 gtgccacccc tcctcgatca gggtctctat gacgagatca ggacgatcga cgaggccgag
     5281 gcgagggtga tggccaggag attggcggcc gaggaaggac tgctcacggg aacctcgagc
     5341 gggctcaacg tcttcggtgc aatcggaatc gcccaggaat tgggggcggg acacaccgtg
     5401 gcaacggtcg ccgtcgacac cggtctcaaa tacctgaccg ggaacctctt cgacgactga
     5461 gccggaagga tcagacccgc tcgacgatca ccaccgggat ctcacgttca gtcttcgtct
     5521 ggtagctgtc gtagtcgggc caaatcgcgg ccatgagctg ccagagccgt gtcttctcct
     5581 cggcagatgc ggttcgggcg cgtcctttga acttctctgc cttcacctgt agctccacat
     5641 cggggttctc cacaaggttc aggtaccagg cgggattctt ggcggcgccg ccgaccgacg
     5701 ccaccagaac gtagcgatca ccgtcgacgc caaagatcaa ggcggtacga cgcaactttc
     5761 cggtcttgcg gccgcggg
//
LOCUS       DBNHXU010000012         1857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2186137, whole genome shotgun sequence.
ACCESSION   DBNHXU010000012 DBNHXU010000000
VERSION     DBNHXU010000012.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2186137"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="AC161X_00310"
     CDS             <1..210
                     /locus_tag="AC161X_00310"
                     /inference="COORDINATES: protein motif:HMM:NF015048.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hotdog domain-containing protein"
                     /protein_id="HIR4988019.1"
                     /translation="ASNAAGDRAVAVATHMAFTSSVRLGDRLEAKVTESARGRTMGTY
                     RVDVTRGDGRTVAVFTGTVHITTSS"
     gene            238..1353
                     /locus_tag="AC161X_00315"
     CDS             238..1353
                     /locus_tag="AC161X_00315"
                     /inference="COORDINATES: protein motif:HMM:NF015755.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR4988020.1"
                     /translation="MGIVQYVIHHPWRVAIMVVAGVLLGLGSFYVYQVQAALGTVASE
                     EFNPQEARAAIDVTPTTSPDIVFVDPAPYPEGEELAEQQLELAGEFDLTGSFDPRLVT
                     PNSFGEPIADEMFTSYLLVGADASGFLADVIIFAIQPEDGSDPIMVSLPRDLFVWNLC
                     KRTLTRLNEGLGGCTGVASGSELLAIMVEDYTGIPVDHVARVNFAGFARIVDAMGGVT
                     ICVDHPTRDAKSHLEITSSGCRRANGEVALAWVRSRHTEQLIGGAWVGVAGSDFARQN
                     RQQDVLFQLAGKASGFSSPISLTNKLSAVSESVRLDSSWTFGQAVSAAWRYRGITKDS
                     VDRFSIEVQNYRAPGGAAVLLPTESFKDQLEAIHPLE"
     gene            complement(1367..>1857)
                     /locus_tag="AC161X_00320"
     CDS             complement(1367..>1857)
                     /locus_tag="AC161X_00320"
                     /inference="COORDINATES: protein motif:HMM:NF021883.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HIR4988021.1"
                     /translation="PILSERLTGMARLLRGYAQVGLENVALWHERDISHSSAERVVLP
                     DASTVLHYMLVKFSGLVERLEVDVDRMKANLDSTRGLVYSQAVLLALVDAGMTRDEAY
                     RIVQMHAMSVWEGEGDLEQRLQSDPQIRLEPELLASCFDPARVSRTATVVFERLDSLR
                     LG"
BASE COUNT          331 a          586 c          591 g          349 t
ORIGIN      
        1 gcttcaaacg cggccggaga cagggcggtt gcagttgcga cgcatatggc cttcacctca
       61 tcggtccgtc ttggcgaccg cctcgaggcc aaagtcaccg agtccgctcg aggccggacc
      121 atgggcacct atcgggtgga cgtgaccagg ggtgacggaa ggacagtcgc ggtgttcacc
      181 gggactgtcc acatcaccac ttcgtcgtga tcgtccagat ctaggatcga ttgcgaagtg
      241 ggaatcgtcc aatatgtgat ccaccacccc tggagggtgg cgatcatggt cgtcgccggg
      301 gtgctcctcg gtttggggtc cttttatgtc tatcaggtcc aggctgccct gggcacggtg
      361 gccagcgagg agttcaatcc gcaggaggcg agggctgcga tcgacgtgac ccccaccacc
      421 tctcccgaca tcgtgttcgt cgacccggcg ccgtatcccg agggcgagga gctggcggaa
      481 cagcagctgg agctggcggg ggagttcgat ctcactggat ccttcgatcc tcgcctcgtc
      541 acgcccaact cgttcggcga gccgatcgcc gatgagatgt tcacctcgta tctcctggta
      601 ggcgcagacg catcaggatt cctcgccgac gtgatcatct tcgccattca acccgaggat
      661 ggctccgacc cgatcatggt gagcctgccc cgcgacctat ttgtgtggaa cctgtgcaag
      721 cggaccctga cccggctcaa cgaagggctc ggcgggtgca ccggggtggc ctcgggctcc
      781 gagctcctcg caatcatggt cgaggactac acgggcatcc ccgtcgacca tgtggcgagg
      841 gtgaacttcg ccggcttcgc ccggatcgtc gatgccatgg gaggggtcac catttgcgtc
      901 gaccatccca cccgagacgc caagtcccac ctggagatca ccagctcggg gtgtcggcgc
      961 gccaatggtg aggtcgcact ggcctgggtc cggtcccggc acaccgagca gctcattggc
     1021 ggcgcctggg tgggggtggc tgggtccgac ttcgcccgcc aaaaccggca gcaggacgtg
     1081 ctcttccagc tggctggaaa ggcctcgggt ttctcttcgc caatctcgct gaccaacaag
     1141 ctgtctgcgg tgtccgaatc agtccgcctc gactcgtctt ggaccttcgg tcaggcggtt
     1201 tcggcggcat ggcgctatcg ggggatcacc aaggactcgg tggaccgctt ctcgatcgag
     1261 gtgcagaact accgggcgcc gggaggggct gcggtcctgc ttccgactga atcgttcaag
     1321 gatcagttgg aggcgattca tcccctcgaa tgaaccgcag caggcgctag cccagtcgca
     1381 gtgaatcgag gcgctcgaac accaccgtgg ctgtcctcga aactcgagcg gggtcgaaac
     1441 acgacgcgag taactcgggt tcgagcctga tctgagggtc actctgcaat cgttgctcca
     1501 ggtcgccttc gccctcccag accgacatgg cgtgcatctg gacgatccga tatgcctcat
     1561 cgcgggtcat cccggcgtcg accagggcga gaagcaccgc ttggctgtac accaggcccc
     1621 tcgtggagtc gagattggct ttcattcgat ccacgtccac ctccagccgc tcgacgagac
     1681 cggaaaactt caccagcatg tagtgaagga ctgtggaggc atctgggaga accacccgct
     1741 cggccgacga gtgggagata tccctctcgt gccagagggc aacattctcg aggccgacct
     1801 gggcatagcc gcgaagaagc cgcgccattc cggtgagccg ctcggagaga atcgggt
//
LOCUS       DBNHXU010000013         8837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2186210, whole genome shotgun sequence.
ACCESSION   DBNHXU010000013 DBNHXU010000000
VERSION     DBNHXU010000013.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8837
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2186210"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..838)
                     /gene="ruvB"
                     /locus_tag="AC161X_00325"
     CDS             complement(<1..838)
                     /gene="ruvB"
                     /locus_tag="AC161X_00325"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HIR4988022.1"
                     /translation="MREDSLLNPVVENEEEVSLRPKRIEEFIGQNRVRERLRISLEAA
                     RKESRALDHVLLSGPPGLGKTTLAGVISHELGVTMRITSGPALERPGDLAAILSNLDD
                     GGVFFIDEIHRLPRAVEEILYPAMEDFQLDIVLGKGPTAQSIRLDLPRFTLVGATTRK
                     GKVTAPLRDRFGIEERLDYYETEDLTRIVIRSASIIDVVIDPVAAEVIASRSRSTPRI
                     ANRLLARVRDYAVARASGTIDPDVADEALGVYEVDQLGLDKVDRSILDAVINRFGGGP
                     VGL"
     gene            complement(843..1412)
                     /gene="ruvA"
                     /locus_tag="AC161X_00330"
     CDS             complement(843..1412)
                     /gene="ruvA"
                     /locus_tag="AC161X_00330"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00084.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HIR4988023.1"
                     /translation="MIGRLRGTLVSKRGEVVCLEVGGVGYDVTMTPRSLATLPGIGEE
                     VVIHTHTHVREDELSLFGFVSEPDRDLFKILLGAAGVGPKLALAMTATLSHDEIIRAI
                     VTEDSDALTAVPGVGKRGAQRIVLELAPKLTGKDAEIVGSPATVTVRQALEGLGYSTE
                     EINGVMPELDPGATIELQITTALRALGRR"
     gene            complement(1409..1891)
                     /gene="ruvC"
                     /locus_tag="AC161X_00335"
     CDS             complement(1409..1891)
                     /gene="ruvC"
                     /locus_tag="AC161X_00335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HIR4988024.1"
                     /translation="MFVLGIDPGLSATGYGLVEGTNPPTARLAGVIRTAVDLPMSERL
                     AELHQGLTSVVADANPDVMAIESVFTNRNLQTAISVGRASGVALLVAAQAGLPVFEYV
                     PTAVKSAITGDGSAGKSQIQEMLARLLHLAEPPQPADSADALAVALCHLRAAPLEARR
                     "
     gene            1962..2387
                     /locus_tag="AC161X_00340"
     CDS             1962..2387
                     /locus_tag="AC161X_00340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009954116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4988025.1"
                     /translation="MEQRISLITLGVSDLGRSRAFYEALGWAAATSPAEDVVFFQTGG
                     MIVGLWSRAELAEDSGVEDSGGWGGVTLAHNVGSPAEVDAVIEEARAAGGTIAREPAA
                     TFWGGYSGVFIDPDGHPWEVAHNPGWILKDDGAIELPSA"
     gene            complement(2388..2759)
                     /locus_tag="AC161X_00345"
     CDS             complement(2388..2759)
                     /locus_tag="AC161X_00345"
                     /inference="COORDINATES: protein motif:HMM:NF015669.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="META domain-containing protein"
                     /protein_id="HIR4988026.1"
                     /translation="MTSPVMSLVGSYWKLLEVDGHPAVAMDELREAHIILLAEEQRLV
                     GSSGCNRIMGTYEVTGDQLRISPLAMTMMMCSEDLMRQEQALTAALEAATGYRISGRS
                     LELFDGEQIVARFEGSDPESS"
     gene            complement(2832..3980)
                     /locus_tag="AC161X_00350"
     CDS             complement(2832..3980)
                     /locus_tag="AC161X_00350"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR4988027.1"
                     /translation="MFEGMEWERVSNDEIDEGVGHFLGLMSGAAAAACEWLIEVDRRQ
                     LFLADGARDLAQWLSARYGLRHSTAVQLVGVARRLQDLPSLRERFASGDLSLDQVDAI
                     SRLATPGSEAELIEETMGLSTAALDRAARRAHPPTSEDERSVWERRFLLIQNSLDGSE
                     GRLHARLPGAEMQIVESAIRSRADAIPPNPETDRFDPYPVRLADGLVEVCATTGDQSS
                     PSVQITIHADLDALTTDTETSGVAELQSGPVIASETARRLACDAIVETAVHDDCRVLG
                     VGRLTRTIPGWLRRQLWHRDGGCRFVGCPHKGWIHGHHIQHWADGGPTDLTNLVLLCS
                     FHHRFLHEHGWTINGDPDTKLTFHKPDGSIYPPPRPELDPRIRELVRT"
     gene            complement(4048..4674)
                     /locus_tag="AC161X_00355"
     CDS             complement(4048..4674)
                     /locus_tag="AC161X_00355"
                     /inference="COORDINATES: protein motif:HMM:NF017959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF998 domain-containing protein"
                     /protein_id="HIR4988028.1"
                     /translation="MITQGDRWLVAGAIWGPGLFILAWVVGGFMMDGYSPIEDHISAL
                     AAVTAPSRFVMNLGFAAYVAGVGSAAWPLRKVIGNGASVFLFVNALLVLGVLFTPDGL
                     SSNADFLHGGFAILTYLTLALVGPLAALTFRRNGRVGWAIGSMAVGTITSLSLWFSLA
                     QTNSGLFQRIGLTTTDLWLMVVGIAYLNGHFFPDRSVGGSTAGGGEGG"
     gene            4723..5004
                     /locus_tag="AC161X_00360"
     CDS             4723..5004
                     /locus_tag="AC161X_00360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020143073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HIR4988029.1"
                     /translation="MGDVLGTIIGGLVAGIIIGPLARLVMPGKQNISLIMTIVIGAVG
                     AIVGGFIADAMDVGATDGIDWIKLAIQVGVAVVAVVLYGSMGGRKTTAT"
     gene            complement(5014..5763)
                     /locus_tag="AC161X_00365"
     CDS             complement(5014..5763)
                     /locus_tag="AC161X_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019137060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR4988030.1"
                     /translation="MSGHSKWANIKHRKGRQDAARGKLFGKLAKAIEIAAREGGGNID
                     FNPTLGTAVFKAKAASMPNDNIERAIKRGTGEVAGAQYEEIWYEGYGPGGVALYVQIL
                     TDNRNRAASDVRSTFTRSGGNLGEPGSVGYLFSQKGLIEAKGTEDEVMLAALDAGAED
                     IRDAGDQWFEVVTSPGDLQKVRSALEAAGLELESADITQLPSTTVPVGEGDARKLLRL
                     IDALDDLDDVQAVFANYDIDDEIMEKVLEEV"
     gene            complement(5763..6326)
                     /gene="pdxT"
                     /locus_tag="AC161X_00370"
     CDS             complement(5763..6326)
                     /gene="pdxT"
                     /locus_tag="AC161X_00370"
                     /EC_number="3.5.1.2"
                     /EC_number="4.3.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007520606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HIR4988031.1"
                     /translation="MTTIGVLALQGDVREHLAILDSLGVNPLPVKKPEQLEAIEALVI
                     PGGESTTIGKMAVRFGLLEPLRSAITDGLPVYGTCAGMILLSGSITEGDQPLLGVLDV
                     VVRRNAFGRQNESFEEDLDIDGLHSPFHAVFIRAPWVEKVGSEVEVLASVGEHPVMVR
                     QDRLLATSFHPELTGDGRIHEMLLELI"
     gene            complement(6328..7221)
                     /gene="pdxS"
                     /locus_tag="AC161X_00375"
     CDS             complement(6328..7221)
                     /gene="pdxS"
                     /locus_tag="AC161X_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020573680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0042823 - pyridoxal 5'-phosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase lyase subunit
                     PdxS"
                     /protein_id="HIR4988032.1"
                     /translation="MEPVVETGTDRVKRGLAEMLKGGVIMDVVTADEAKIAEEAGAVA
                     VMALERVPSDIRKDGGVARMADPTKIEEIQASVTIPVMAKCRIGHFAEAAILQALGVD
                     YIDESEVLTPADEEHHVDKWAFTTPFVCGARNLGEALRRIGEGAAMIRTKGEAGTGNV
                     VEAVRHIRTVTNEMRALTTMDEAQLMSAARDHQAPYDLVREVASTGKLPVVNFAAGGI
                     ATPADASLMMSLGVDGVFVGSGIFKSSNPEAYARAIVEATTFWNEPEVVAKVSRGLGE
                     AMPGIELGTLTETERLASRGW"
     gene            complement(7261..8292)
                     /locus_tag="AC161X_00380"
     CDS             complement(7261..8292)
                     /locus_tag="AC161X_00380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR4988033.1"
                     /translation="MSLRVGLVSPYDLSVPGGVQSQVLGLAGYLEGEGIEPVVIGPGL
                     PPEVTGVDLGRTVAVPGNGSKVPIALDPRIRSKVREAAVEVDLLHVHEPLMPLVSLFA
                     TRSGPPVIATFHAAPGAVGRLAYRVTGPIMSWLLGNTAAVTAVSRTAASSLPKALQVR
                     IIPNGLNVGAMKTDVPRDPTRVSFLGRDEPRKGLDVLLAAWPKVSEAVPGATLTVMGA
                     DRGNAGAEWLGRVDHPTKAQVLGSSAVFVAPQLGGESFGIVLLEAMASGAAVVASNLD
                     AFVEVADGAARFFPIGDSTALARGIVDVLGDPLECARLASVGFEVAARYDWQLVGASY
                     RSLYDEIAS"
     gene            complement(8289..>8837)
                     /locus_tag="AC161X_00385"
     CDS             complement(8289..>8837)
                     /locus_tag="AC161X_00385"
                     /inference="COORDINATES: protein motif:HMM:NF015251.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LpxL/LpxP family acyltransferase"
                     /protein_id="HIR4988034.1"
                     /translation="KGMIFALPHMGNWDVAAPVAVYEGIPVVAVAERLANTRITDWFT
                     KMRAGFGIEIVLATGSTEVMRKLEEALANNKGVALLADRDLKGRGVEVEFFGEMTTLP
                     PGPATLALRTGAPIFPITSFFRPKGHRLEVTSPISIPETGTRTEKIQVMTQELAYRME
                     KMISEDPQQWHLVVPNWPSDRE"
BASE COUNT         1663 a         2810 c         2791 g         1573 t
ORIGIN      
        1 aaagcccgac cgggccccca ccgaaacggt tgatcacggc gtcgaggatc gaacggtcga
       61 ccttgtccag acccagttgg tcgacctcgt agacgccgag tgcctcgtcg gcgacgtcgg
      121 ggtcgatcgt gcccgaggct ctggcgacgg cgtagtcacg gacccgggcc agtagccggt
      181 tggcgatccg cggtgtcgat cgacttcgtg aggcgatcac ctcggcggcg accggatcga
      241 tcacgacatc gatgatggag gcggagcgaa tcacgatgcg ggtcaagtcc tcggtctcgt
      301 agtagtcgag tctttcctcg atcccgaagc gatcacgcag cggtgcggtg accttccctt
      361 tccgggtggt cgcgccgacc agagtgaatc gaggcaggtc gaggcggatc gattgcgcgg
      421 tggggccctt gccgaggacg atgtccagct ggaagtcctc cattgccgga tagaggatct
      481 cttcgacggc acggggcaga cggtgaatct catcgatgaa gaacacgcca ccgtcgtcga
      541 ggttggagag aatggccgcc aggtcaccgg gccgctccag ggcggggccg gaggtgatcc
      601 gcatcgtcac cccaagctcg tgggagatca caccggcaag ggttgtcttt cccaaaccgg
      661 gtgggccgct gaggaggacg tgatcgaggg cacgactctc tttacgtgcc gcctcgagcg
      721 agatccgcag ccgctcccgc acccggttct ggccgatgaa ttcttcgatc cgcttcgggc
      781 gaagcgatac ctcttcctcg ttctcgacaa ctggattgag aaggctgtcc tctcgcatgg
      841 gcctacctcc gaccgagtgc cctgagggcg gtcgtgatct gaagttcgat ggtggcaccg
      901 ggatcgagct cgggcatgac accgttgatc tcttcagtgg agtatccgag cccttccaga
      961 gcctgacgaa cggtgacggt ggccggggag ccgacgatct cggcgtcctt gccagtgagc
     1021 ttcggtgcca gttcgaggac tattcgttgc gctccacgct tgccgacacc gggcacagca
     1081 gtcaaggcgt ccgaatcctc agtgactatg gctcgaatga tctcgtcgtg actgagggtg
     1141 gccgtcatgg ccagcgccag cttgggcccg actcctgccg cacccaggag gatcttgaac
     1201 aggtcacgat caggctcgga gacgaagccg aagagactca gttcgtcctc acggacatgg
     1261 gtatgagtat ggatcaccac ctcctcgccg atcccgggaa gagtggcaag gcttcgaggc
     1321 gtcatcgtca cgtcgtatcc gaccccaccg acttcgaggc agaccacttc gccgcgcttc
     1381 gagaccaggg tgccccgcaa tcgcccgatc atcgtcttgc ctcgagaggg gcagcccgca
     1441 aatggcagag ggcgacggcg agggcgtcgg ccgagtcggc cggttgtggc ggctcggcga
     1501 gatggaggag gcgagccagc atctcctgga tttggctctt tcccgccgac ccatccccgg
     1561 tgatcgccga tttcaccgcg gtgggcacgt actcgaacac cggcagacct gcctgggcgg
     1621 cgaccaggag agccacccca gaggcgcgac cgaccgagat cgcggtctga aggttgcggt
     1681 tggtgaagac actctcgatt gccatgacat cggggttggc gtcggccacg acgctggtca
     1741 gcccctgatg cagctcagcg aggcgctccg acatcggaag gtcgacggca gtgcggatga
     1801 cacccgcgag ccgagcggtc ggaggattcg tgccctccac cagtccatat ccagtggcgg
     1861 acagcccggg atcgatgccc aatacgaaca tgtgttctac gacagtacct gctctggctg
     1921 acgtttccga gccttgttgg gaacatgcga gaatccgcca aatggagcag cggatcagcc
     1981 tgatcacact cggtgtgtcc gatctcgggc gttcgcgggc gttctacgag gctctcggct
     2041 gggcggccgc cacctcgccc gccgaggacg tggtgttctt tcagaccgga ggcatgatcg
     2101 ttggattgtg gagccgcgcc gagctggcgg aggacagcgg tgttgaggac tccggcggct
     2161 ggggcggggt caccctggct cacaacgtgg gctccccggc cgaggtcgac gctgtgatcg
     2221 aggaggcccg ggcagccggg ggaaccattg cgcgtgagcc cgcagcgacg ttttggggtg
     2281 gctactccgg tgtcttcatc gaccctgacg ggcatccatg ggaagtggct cacaacccgg
     2341 gatggatact gaaggatgac ggcgctatcg agctgccctc ggcctgatca gctcgactcg
     2401 gggtcgctcc cctcgaatcg ggcaacgatt tgctcgccat cgaatagttc cagcgaccgt
     2461 cccgaaattc gatacccggt tgccgcctcc aacgctgcgg tcaacgcttg ctcctggcgc
     2521 atcaggtcct cggagcacat catcatggtc atggcgagcg gtgagattcg cagctgatcc
     2581 cctgtcacct cataggtgcc catgatccga ttgcaaccgg aggacccgac gagtcgctgc
     2641 tcctccgcca gcaagatgat gtgagcctcc cgcagctcat ccatcgctac cgcggggtgc
     2701 ccgtcgacct cgagcagctt ccaataggaa ccgaccaggc tcatgacagg tgaggtcata
     2761 gtccgatcct gccacatggc tcgcagtgtg gatgatgtcg atggtcgggg tggtggtggg
     2821 ggcggtggcc cctaggttcg gacaagttct cggattcggg ggtcgagttc tggtcggggt
     2881 ggcgggtaga tgctgccgtc gggcttgtgg aaggtgagtt tggtgtcggg gtcgccgttg
     2941 atggtccagc cgtgttcatg gaggaaccga tggtggaagg agcagagcag gaccaggttg
     3001 gtgaggtcgg tgggcccacc atcagcccaa tgttggatgt ggtggccatg gatccaaccc
     3061 ttgtggggac atccgacgaa ccgacagcca ccgtcccgat gccagagttg gcgtcgtagc
     3121 cagccgggaa tggtccgggt gagccgacca actcctagga cccggcagtc atcgtggact
     3181 gcggtctcca cgatcgcatc acaggccaaa cgtcgtgccg tctctgaggc gatcaccgga
     3241 ccggactgga gttcggccac gccgctggtc tcggtgtccg tggtgagcgc gtcgaggtcg
     3301 gcgtggatcg tgatctggac actcggggag gattggtcac cggtggtggc gcagacttcg
     3361 accagcccat cggcgagccg gaccggataa gggtcgaacc ggtcggtttc agggttgggc
     3421 gggatcgcat cggcacggga ccggatcgca gattccacga tctgcatctc cgcaccgggt
     3481 agacgggcgt ggagtcgacc ctccgaaccg tccaaactgt tttgaatcag taggaaccgg
     3541 cgctcccaca ccgaacgctc atcctcactg gtaggcgggt gggctcgtcg ggccgcccga
     3601 tccaacgcgg ccgtcgacaa acccatggtc tcctcgatga gttcggcctc ggagccgggg
     3661 gtcgccagcc gggagatcgc atccacctga tccaacgaaa ggtcaccaga agcgaagcgt
     3721 tctcggagtg aaggcagatc ctgcagacgg cgggccaccc ccaccagttg gaccgcagtc
     3781 gaatgcctca atccgtaacg ggctgacagc cactgagcca gatcccgtgc accatcagcc
     3841 aagaacaatt ggcgacggtc cacttcgatc agccactcgc acgccgccgc agccgccccc
     3901 gacatcaaac ccaggaaatg gccgaccccc tcatcgatct catcgttgga aaccctctcc
     3961 cactccatac cctcgaacat acgttcggcc agggacagaa acaagaggat tgacgaccga
     4021 aactgagaat attttctacc cgccgagcta gcccccctcc ccgcctccgg cggtactccc
     4081 cccaacgctt cggtcgggga agaaatggcc gttcaagtag gcgatcccga ccaccatcaa
     4141 ccacagatcg gtggtggtga gcccgatccg ctggaacaga ccggaattcg tttgtgcgag
     4201 cgaaaaccaa agggacaacg aggtgattgt cccgaccgcc atcgaaccga tcgcccatcc
     4261 cacccggcca tttcgccgaa aggtcagggc ggccagcggc cccaccaacg caagagtgag
     4321 gtaggtgaga atcgcgaagc cgccgtgcaa gaagtcggcg ttggacgaga gaccatcagg
     4381 ggtgaagagc acgccgagca ccaacagggc gttgacgaag aggaagactg aggcaccgtt
     4441 gccgatcacc tttcgcaacg gccaggcggc cgatccgacc ccagcgacat atgccgcgaa
     4501 tccgagattc atcacgaaac gggaaggagc agtgaccgcg gccaaggcac tgatgtggtc
     4561 ctcgattggc gaatagccat ccatcatgaa accgccgacc acccaggcca ggatgaagag
     4621 acctggaccc cagattgccc cagccaccag ccagcggtcg ccttgtgtga tcacgatcgg
     4681 agagcgtagg gtccgagcgt tcacgacttc taaggagcgg aaatgggaga tgtcctcgga
     4741 acgatcatcg gcggcctcgt agccgggatc atcatcggcc cactggcccg tctggtcatg
     4801 ccaggcaagc aaaacatctc gctgatcatg accatcgtca tcggggcagt cggcgccatc
     4861 gtcggcggat tcattgccga tgcgatggat gtcggcgcca cggacgggat cgactggatc
     4921 aagctggcaa ttcaggtcgg cgtggcagtg gttgccgtgg tcctctatgg gtcgatgggc
     4981 ggacgcaaga cgaccgcgac ctagaaccgg gcctcagacc tcttcgagaa ccttctccat
     5041 gatctcgtcg tcgatgtcgt agttggcgaa cactgcctgc acgtcatcga ggtcatccag
     5101 ggcatcgatg agccgtagaa gcttccgggc atccccttct cccaccggca ccgtggttga
     5161 ggggagttgg gtgatgtcgg ccgactccaa ttcgagaccg gcggcctcca gggccgagcg
     5221 gaccttttgc aggtcgccag gcgaggtgac cacttcgaac cactgatcgc cggcgtcacg
     5281 gatgtcttcg gctccggcgt cgagggcagc cagcatgacc tcatcctcgg tgcccttggc
     5341 ctcgatcaga cccttctgac tgaataggta cccgaccgat cccggctccc ccagattccc
     5401 cccgctgcgg gtgaacgtcg atctcacgtc cgatgctgcc cggttgcgat tgtcagtgag
     5461 gatctgcacg tacagggcga caccccccgg cccgtatccc tcgtaccaaa tctcctcgta
     5521 ctgggctccg gcaacctcgc cggtgccccg cttgatggcg cgttcgatgt tgtcgttggg
     5581 catcgaggcc gccttggcct tgaacaccgc cgtgccgagg gtggggttga agtcgatatt
     5641 cccaccgcct tcccgggcgg cgatctcgat tgccttggcc agcttcccga agagctttcc
     5701 ccgggccgcg tcctgacgac ccttgcgatg cttgatgttg gcccatttgg agtgcccgga
     5761 catcagatca gctccaacaa catctcatga atccgcccat cacccgtgag ctcagggtga
     5821 aaggacgtcg ccagaagccg atcctgacgc accatcaccg gatgctcgcc cacggaagcc
     5881 aggacctcga cctccgaacc gaccttctcg acccaggggg cgcgaatgaa caccgcgtga
     5941 aacggggagt ggaggccgtc gatgtcgaga tcttcctcga acgactcgtt ctgacggccg
     6001 aaggcgttgc gccgcaccac gacgtcgaga accccgagga ggggctggtc gccctcggtg
     6061 atcgaccccg aaagaaggat catcccggca catgtcccgt agacgggcag cccgtcggtg
     6121 atcgccgaac gaagcggctc gagtaggccg aagcgaaccg ccatcttccc gatggtggtg
     6181 gattcgccgc cagggatcac aagtgcctcg atcgcttcaa gctgctcagg tttcttcacc
     6241 ggaagcggat tcaccccaag tgaatcgagg atcgcgaggt gctcccggac gtccccttgc
     6301 aaggcgagga cgccgatcgt ggtcatctca ccaacctcgg ctggccaggc gctccgtctc
     6361 ggtcaatgtt cccaattcga tgccgggcat tgcttcgccc agaccgcggg agaccttggc
     6421 aactacctca ggctcgttcc agaacgtcgt ggcctcgacg atcgccctgg cgtacgcctc
     6481 gggattcgag ctcttgaaga tcccggagcc aacgaacaca ccatcgacgc ccaacgacat
     6541 catcagtgag gcgtcggcag gagtggcgat gccgccagct gcgaagttca ccacggggag
     6601 cttgcccgtg gacgccactt cgcgcaccag gtcataggga gcctgatggt cacgcgccgc
     6661 cgacatgagc tgggcttcat ccatcgtggt gagagcgcgc atttcgttgg tgacagtgcg
     6721 tatatggcga actgcctcga ccacattccc ggtgccggcc tcgcccttgg tgcggatcat
     6781 ggcggcacct tcgccgatac ggcgcaacgc ctcacctagg ttcctggcgc cacagacaaa
     6841 tggggtggtg aaggcccatt tatcgacatg gtgctcctcg tcggccggag tgaggacctc
     6901 ggactcgtcg atgtagtcga ccccgagcgc ttgaaggatt gctgcttccg cgaaatgccc
     6961 gattcggcac ttggccatga cggggatggt caccgacgcc tggatctctt cgatcttggt
     7021 ggggtcggcc atgcgggcca ctcccccgtc cttacggatg tcggagggga cccgttccag
     7081 ggccatgacc gctaccgcgc cggcctcttc ggcgatcttt gcctcgtcgg cggtcaccac
     7141 gtccatgatg acgccgccct tcagcatctc cgccaggcct cttttcacac gatctgtacc
     7201 ggtttccacg acaggctcca ttaacgggaa aggccatggt acagactcgg cggcaatgaa
     7261 ctaagaggcg atttcgtcgt agagggagcg ataggaggct cccaccagtt gccagtcgta
     7321 gcgggcggcc acttcgaaac cgaccgatgc cagccgagca cactcgaggg ggtcacccag
     7381 gacatccacg atccccctgg ccagggccgt gctatccccg atggggaaga atcgggcagc
     7441 gccatcggcg acctctacga aggcgtcgag gttcgaagca acaactgccg cccctgatgc
     7501 catcgcctcg agcaggacaa tcccgaagga ttcgcccccg agttgggggg cgacaaagac
     7561 cgccgacgag ccgaggacct gcgcctttgt cgggtggtcg acacggccaa gccactccgc
     7621 gccggcgttg ccccgatcgg cccccatcac cgtcagggtc gcgccgggaa ctgcctcgga
     7681 caccttgggc catgccgcaa gcagaacgtc aaggcctttg cgcggctcgt ccctgcccag
     7741 gaaggacact cgagtcggat cgcggggaac atcggtcttc atcgcaccga cattcagccc
     7801 gttgggaatg attcgaacct gcagcgcctt cgggagcgat gacgctgcag tcctgctgac
     7861 ggcggtcacc gccgcggtgt ttcccaatag ccagctcatg attggcccgg tcacccggta
     7921 tgccagccgc cccacggcgc caggagcggc gtgaaaagta gcgatgacgg gcgggccgga
     7981 tcgagtggcg aacagggaga cgagtggcat cagcggctca tggacgtgaa ggagatcaac
     8041 ctccacggcg gcctcacgaa ccttcgagcg aattctggga tccagggcaa tcgggacctt
     8101 ggagccgttg cccgggaccg ccacagttcg cccgaggtcg acaccggtca cctccggggg
     8161 caagccgggc ccgatcacca ccggctcgat cccctcaccc tcgagatacc cggccagacc
     8221 taggacctga gattggactc cccccgggac cgacaggtcg taggggctca ccaggccgac
     8281 tcgcaagctc attcgcggtc agacggccag ttgggaacca ccagatgcca ttgctggggg
     8341 tcctcggaga tcatcttctc catgcggtag gccaattctt gggtcatcac ctggatcttc
     8401 tccgttcggg tcccggtttc aggtatcgag attggagacg tgacctccag ccgatggcct
     8461 ttgggccgga agaaggacgt gatagggaag atcggggccc cggtcctgag ggccagggtc
     8521 gccgggccgg gagggagtgt cgtcatctca ccaaagaact cgacctcgac gccccgcccc
     8581 ttgagatcac ggtccgccag cagggctact cccttgttgt tggcgagggc ttcctcgagc
     8641 ttcctcatca cttcggtgct tcccgttgcg agcacgatct cgatcccgaa gccggctctc
     8701 atcttggtga accagtcggt gattcgcgtg ttggcgaggc gttccgccac agcgacgacg
     8761 ggaatcccct cgtaaaccgc aaccggcgcc gccacgtccc agttccccat gtggggaagc
     8821 gcgaagatca tgccctt
//
LOCUS       DBNHXU010000014         2766 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2385068, whole genome shotgun sequence.
ACCESSION   DBNHXU010000014 DBNHXU010000000
VERSION     DBNHXU010000014.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2385068"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="AC161X_00390"
     CDS             <1..1264
                     /locus_tag="AC161X_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011320765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR4988035.1"
                     /translation="AGQEPAPGTNARAVPIYQTTSYTFNDAAHAARLFGLEEFGNIYT
                     RIMNPTTDVFEKRVAALEGGLAAVATSSGQAAQLLAFTTLLETGDHFVSTSYLYGGTY
                     NQFKVAFPRLGFNVSFVDGDDPADFEKAITEKTKALYVETIGNPKYNIPDFEALAEIA
                     HRNGIPLVVDNTFGAAGYISRPIDFGADIVVHSATKWIGGHGTSIGGVIVDAGNFDWR
                     NGKFPIFTEPSPGYHGLVFTDVFNPDGPFGNIGFAIRARVEGLRDFGAALSPFNSFLF
                     LQGLETLSLRVQRQVDNALELAKWLESHALVDWVSYPGLPSHPYHEAAKKYLRNGFGG
                     VLSFGIAGGFEAGKRFIDATKLASHLANVGDAKTLVIHPASTTHQQLSEEEQISTGVV
                     PELVRISVGIEHIEDIKEDVDQALEAVS"
     gene            1275..2414
                     /locus_tag="AC161X_00395"
     CDS             1275..2414
                     /locus_tag="AC161X_00395"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006514440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016413 - O-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-acetyltransferase family protein"
                     /protein_id="HIR4988036.1"
                     /translation="MSKPGPSGQVVETRFFDLPPDSLPFKMRSGDLLPAVRLAYELQG
                     EISPEGDNVILLFHALTGSQHFAGFTPSVEGVGRWVEENQTGWWEGFVGSGLGIDTDK
                     YAVLCVNYLGGCYGTTGPESIDPRTGQRYGPAFPPLHLADILESQLPLLKHLGITRLK
                     AVAGASVGALLSLSLASHYPDLVENVIILAGGLAGTTLHRMYNFEQKLAIFNDPDFNS
                     GDYYDGAFPADGLALARIISHKAFVSLGAIEERARNEIIDHPYVTHPVESYFWNQGQK
                     LVIRFDPNSYLRIIDSWSNYDLVEDTGAASYVDALVPCRDQNWHVFTIDTDVCFYPAE
                     QAGLVSALEEAGVPVEWIRVTSPKGHDSFLTEPHLFEDALRKALA"
     gene            complement(2411..>2766)
                     /locus_tag="AC161X_00400"
     CDS             complement(2411..>2766)
                     /locus_tag="AC161X_00400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988037.1"
                     /translation="RTVNGCRGLMAEMSPLRRRSWSIGATVLGFAMLAAYVTLILSEG
                     GNTLGTTMPWALLMLIAAAIALVSALVADPRLARKLLLGAGVLYGLLGVVSILTIGIG
                     FLAAAGVSLIGASKV"
BASE COUNT          539 a          874 c          848 g          505 t
ORIGIN      
        1 tgcggggcag gaacctgccc cgggcaccaa tgcccgggcg gttccgatct atcagaccac
       61 cagctacacg ttcaacgacg cggcccacgc cgctcgtctg ttcgggctgg aggaattcgg
      121 aaacatctac acccggatca tgaaccccac caccgacgtc ttcgaaaagc gtgtggcggc
      181 gctggagggc gggttggctg cggtggcaac gtcgtcagga caggcggcgc agctgctcgc
      241 cttcacgacc ttgctggaga cgggtgacca tttcgtgtcg acctcgtacc tgtacggagg
      301 gacttacaac cagttcaaag ttgcgttccc ccgcctcggg ttcaatgtca gcttcgtcga
      361 cggcgacgac ccggctgact tcgagaaagc gatcacggag aagaccaagg cgctgtacgt
      421 cgagacgatc ggcaacccga agtacaacat cccggacttc gaggctctgg ccgagatcgc
      481 ccatcgcaac ggcatcccgt tggtggtgga caacaccttc ggggctgccg ggtacatctc
      541 gcgaccgatc gacttcggcg cagacatcgt ggtccattcg gcgacgaagt ggatcggcgg
      601 tcacggaacc tcgatcggtg gagtgatcgt cgacgccggc aacttcgact ggcgaaacgg
      661 gaagttcccg atcttcaccg agccgtcccc cgggtaccac ggtctggtct tcaccgacgt
      721 gttcaaccct gacgggccgt tcgggaacat cgggttcgcg atccgggcga gagtcgaggg
      781 tcttcgtgac ttcggtgccg ctctgtcgcc gttcaactcg ttcctgttcc ttcagggtct
      841 ggagaccctg tcgctgcgcg tgcagcgtca ggtcgacaac gcactcgagc tcgcgaaatg
      901 gctcgagtcg catgctctcg tcgactgggt cagctacccg ggcctgccct cgcatcccta
      961 ccatgaggcc gcgaagaagt acctgcgcaa cgggttcggc ggggtgttgt ccttcggcat
     1021 cgccggtgga ttcgaggccg ggaagcggtt catcgacgcc accaagctgg ccagccattt
     1081 ggccaatgtc ggggatgcca agaccctcgt gatccatccg gcatcgacca cccatcagca
     1141 gctgtccgag gaagagcaga tctcaacggg ggttgtgccc gagctggtga ggatctcggt
     1201 gggaatcgag cacatcgagg acatcaagga ggacgtcgac caagccctgg aggcggtctc
     1261 ctaaggagat aggtatgagc aagccgggcc cgagcgggca ggtcgttgag acccgtttct
     1321 tcgacctgcc accagactcg ctgccgttca agatgcgcag cggggacctg ttgcccgcgg
     1381 tccggcttgc ctacgagctc cagggagaga tctccccgga aggggacaac gtcatcctgt
     1441 tgttccacgc tttgacaggc agtcagcatt tcgccgggtt caccccatcc gtggagggag
     1501 tgggccggtg ggtggaggag aatcaaacgg gctggtggga gggtttcgtc ggtagcgggc
     1561 tagggatcga caccgacaaa tacgcggtcc tgtgtgtcaa ctatctgggc ggttgctacg
     1621 gcacgacggg cccggaatcg atcgaccccc ggaccggcca gcggtacggg cctgcctttc
     1681 cgccgcttca tctggccgac attctcgaat cgcagctgcc tcttctcaag catctcggga
     1741 tcacgcgact gaaagcggtg gccggagcct cggtaggagc cctgctctcc ctgtcgctgg
     1801 catcccacta cccggatctt gtcgagaatg tgatcatcct cgccggtggg cttgccggca
     1861 ccacgttgca ccggatgtac aacttcgagc agaaactggc gatcttcaac gatccggact
     1921 tcaacagtgg cgactactac gacggcgcct tccctgcgga cggactagcc ctggcccgga
     1981 tcatcagcca caaggcgttc gtgtctttgg gggcgatcga ggagagagct cgcaacgaga
     2041 tcattgacca cccctatgtc actcacccgg tcgagtccta cttctggaat caaggccaga
     2101 agctggtgat tcgcttcgat cccaacagtt atctcaggat catcgattcc tggtccaact
     2161 acgacctggt cgaagacact ggagctgcga gctacgtcga cgccctcgtc ccctgccgcg
     2221 accagaactg gcacgtcttc accatcgaca ccgacgtctg tttctacccc gcggagcagg
     2281 ctgggctggt gtcagccctg gaggaggcgg gggtcccggt ggagtggatc cgggtcacat
     2341 cacccaaagg gcacgactcg tttctcaccg agcctcatct cttcgaagat gcgttgcgca
     2401 aagcgctggc ctagaccttc gaagcgccga tcagggatac tcccgcggca gccaggaatc
     2461 cgatgccgat cgtcaggatg ctcacaacac caaggagccc gtagagaacc ccggcaccca
     2521 acagcagttt gcgggcgaga cgagggtcgg ccacgagcgc agacaccaat gcgatcgcag
     2581 ccgcgatcag catcaacaga gcccagggca tcgtggttcc cagcgtgttc cccccctctg
     2641 acaagatgag ggtcacgtag gcggccagca tggcgaaccc aaggaccgtc gcgccgattg
     2701 accaggaccg acgtcgcaga ggactcattt cagccatgag accacgacac ccattcaccg
     2761 tccgac
//
LOCUS       DBNHXU010000015         9879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3774278, whole genome shotgun sequence.
ACCESSION   DBNHXU010000015 DBNHXU010000000
VERSION     DBNHXU010000015.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9879
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3774278"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..690
                     /locus_tag="AC161X_00405"
     CDS             <1..690
                     /locus_tag="AC161X_00405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018731711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4988038.1"
                     /translation="LADEINRAPAKVQSALLEAMGERQISIGGTTYRLPLPFLVLATQ
                     NPIESEGVYPLPEAQRDRFLMRIPVDYPTAVEEHEIVDRSRRQTAAPASLLSTDDVAS
                     LQTAAESVYVDRSVQDYAIRLVLATRNPSEHGVKIDGLLAYGASPRASIGLVAAGRAL
                     ALLRGRNHLLPQDVYDIAYDVLNHRLVLSFDAVADGVTVDEVLIELLTTVVAPRLSSS
                     QLIEQRSPVGV"
     gene            693..1661
                     /locus_tag="AC161X_00410"
     CDS             693..1661
                     /locus_tag="AC161X_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014350856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR4988039.1"
                     /translation="MLGRSGPPSGVEAIPRVSRLTSPSRVPIDRLRMTVLRRLDGLLS
                     GDHAGLFPGHGTERGEARPYVPGDDPRHIDWAVTARTHDPHVRDTIADHELEVWLVLD
                     TSSSLGFGTGLATKHEVAWSAAGAFALLASSGGNRVGAVRSTTDGPASRPQILPARSG
                     SSHTGAVLAALYRAPVDGEAGDLGAAIDMVRRTARRRGLAVVISDFLGEPTWERSLRA
                     LSHRHELIGVEVVDPREVDLPDVGLISVVDPETGRRRLVDTSTSSVRRAYGELAVRRR
                     ADIAARLKSCGADQLTLRTDRDWVLDFVRFVSGRKARLMAAGRSTR"
     gene            1658..2458
                     /locus_tag="AC161X_00415"
     CDS             1658..2458
                     /locus_tag="AC161X_00415"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR4988040.1"
                     /translation="MSDFGFLVPGRLALLVLPIAMGLAYLVLQARRRRYAMRFTTIEM
                     LDQVAPDRPGWRRHLTALGLVLAVVVATVAFARPVIAGETYESVQTVVLAIDTSLSME
                     ADDVSPSRIDAVRDAAGEFLDTVPEGVSVGVVAFDGEARELIAPTERLDAVRRVIDKS
                     IDHGDLGEGTAIGEAVFAGINAIESSAPDLEATTPETGESVGTIVVLSDGETTMGRTN
                     DEAATAARNASIPVHTIAFGTDAGTIVDPYGEVVPVPVNEAALRKLAD"
     gene            2507..2644
                     /locus_tag="AC161X_00420"
     CDS             2507..2644
                     /locus_tag="AC161X_00420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988041.1"
                     /translation="MYEDLGRSVQVETEPVEIADWFIGAALAVLSLAGLGSLVWFGRL
                     P"
     gene            complement(2656..3996)
                     /locus_tag="AC161X_00425"
     CDS             complement(2656..3996)
                     /locus_tag="AC161X_00425"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4988042.1"
                     /translation="MPVSVTDFPDTRYAKSGDISIAYQTIGNGPDLIFCSGLFSNVDV
                     MWEEPHWEHLLGRMASFSRLTVFDMRGIGQSDRGSEAPILERQRDDLGAVMDAVGVED
                     AIIFGVARAASMSTLFAATHPQRTEGLILYAPTAKRVASSDFSVGISAEEQASFLDRF
                     VREVGTGKNLSIQAPSLAHDERFVKWWARFERLVATPAAYRELGQILTDLDIRDVLPA
                     IQVPTLVLHREGDLIVPRDQARFVADQIAGARYVELPGVDHLPIAGDSDAILDVVEEF
                     VTGKRQVPRTDRILATVMFTDIIDSTSLQASLGDHEWKRLLEAHHRIVRRQLERHGGR
                     EQDTAGDGFYIVFDGPARAIECARDVMTDLAPLGVQIRVGVHTGECEIADGKCAGLAV
                     SIGARVMGQAGASEILVSQTVKDLTAGGDLHFREAGEHELRGIPDRWRLYRVAA"
     gene            4200..4847
                     /locus_tag="AC161X_00430"
     CDS             4200..4847
                     /locus_tag="AC161X_00430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR4988043.1"
                     /translation="MKPVHISIVLDRSGSMSRIADDVVGGFNQFLRKQRETEGEARVT
                     LVQFDSDDPFELLIDGVPLREVIDLDRTGYQPRSTTPLYDAVGRMIVRIDGVIVSRNE
                     RALPEEDQVVLIVTDGLENASTDFDRSKVFEMVSDRRKQGWVFVFLGADQDVYAEGGK
                     VGVATANRVAWEKSKAGSDKMWKDIAYSTTTYREKEEFLRRQDRDRFHEEDPDKE"
     gene            complement(4877..5779)
                     /locus_tag="AC161X_00435"
     CDS             complement(4877..5779)
                     /locus_tag="AC161X_00435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988044.1"
                     /translation="MATLRWLGKSESLLFSLVTLLLVACATPPTSAATTTTGGKQAVP
                     TTTTIKEEVTESTEIAISTTTIETPTTSTTAPESITVSDYPESWAQALVDAANQDNFG
                     LLDSYAFVSDQKRELFRVFVESDLPELELGSCEQNEFYSGCSFGSADKQMYRVELDLD
                     SGSELFEDLLIRAQGIDDLASDGAYGSGCSPGPGPLTDGIWFGYIAARRSDAIDFDLA
                     CLLPNDWVDVEMVNSSAALRTLPVEGDLLVTQMIVSEFESESFAGTLYRDWGAEWCSV
                     TGTCAVWIRIEGGRVIYIAEEFYA"
     gene            5872..6249
                     /locus_tag="AC161X_00440"
     CDS             5872..6249
                     /locus_tag="AC161X_00440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988045.1"
                     /translation="MEPETRYARLGEDRIAYQVVGDGPVDLVISRGTFSVLDAIWGTS
                     ETAAPYLQLAAVCRLILFDRLGSGWSDAVPLDTLPPLESRWAESVRSWMRLRVNGRSS
                     WGSRTAGRRSCSVRQPNPIEWLA"
     gene            6207..7262
                     /locus_tag="AC161X_00445"
     CDS             6207..7262
                     /locus_tag="AC161X_00445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4988046.1"
                     /translation="MFGAATQPDRVAGLILTHSPARSRVDVDYPFGYDEATIAEQSKM
                     VAEWDLDTMISRSFPSRGNDERFLQWGRRYMRGMASPTAMRAYTEEMGQIDVRDLLPS
                     IRVPTLVLHRREFAWAPPELSRYVADHIAGARYVELPGGDADFFFDETAPMLDAITGF
                     LAEIEPRTIQRRSAERMMATILFTDIVSSTERAQASGDAKWVVQLQLHDDMSRDLIGK
                     HGGRLVKSTGDGVLALFDGPGRGLLAAGDMCSSLDRAGMPVRAGLHAGEIELRGRGCG
                     RGRGSHRSAGDGRSRTGRGVGLQDSSRSRGRLRLSIRGSGLAPSQRCGRRVAAVRPSR
                     ALIIGRPDVRNLPVYKA"
     gene            complement(7250..8527)
                     /locus_tag="AC161X_00450"
     CDS             complement(7250..8527)
                     /locus_tag="AC161X_00450"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR4988047.1"
                     /translation="MGAIHRFDRWWFPEWLVDGKAPSGKHEDTMQQDGSGGTPPGRRR
                     LADLADATPDKRDRYVDFLRALSVVVVVLGHWLGAVVYLQDGELTATSALDVVPGLWI
                     ITWALQIMPIFFFVGGFSNFVTYEGLQRLGEGPGVFLKGRASRLLRPVGIFLLFWAIV
                     APFALQLDLPDGAAEKAVLVLLGPLWFICAYLMLVLMAPVMVFLHRKFRIRVLVALVL
                     IPVAIDLLRFAGDVPVIGWFNFLFVWLFIHQFGFFYADGTFDRVSRSLFWIMAIGGFA
                     AMVGLTQMQVYPASMVGCCSDEISNMAPPTLAIMVFGVYQVGLSMLLRPSLTKWLARK
                     RPWSVVIAINSSIMTLFLWHLSALVIAASILLPLGFPQPEMGSVQWWLTRPLWFGAAG
                     LVLALLVFIWGRFERPDLDPAEQYWDLEEFYAL"
     gene            complement(8479..9207)
                     /locus_tag="AC161X_00455"
     CDS             complement(8479..9207)
                     /locus_tag="AC161X_00455"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR4988048.1"
                     /translation="MPDRGRFLVLGAVGGYNLVQNYMVSERAYVPANMVATLGLVALA
                     RRNGCTWDELGLNPLEAGRGIRHGLAGAAGAAVVAGGITTLRSARPYLLDERAAGHSA
                     AEITYRAAVRFPLGTALFEEVAFRGVIYGTWRHSGASPRTAAVVTATAFGLWHLIPSG
                     QALRGNPLGARIGTRCAEAGVVMAGALLTGISSLGFGWMRRRSGSLVAPWIGHAAINS
                     FTYLAGVGAWGRSTASTGGGSLSG"
     gene            complement(9200..>9879)
                     /locus_tag="AC161X_00460"
     CDS             complement(9200..>9879)
                     /locus_tag="AC161X_00460"
                     /inference="COORDINATES: protein motif:HMM:NF048150.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR4988049.1"
                     /translation="LGLHQGWGLTETAALCAVQRPDDLEIGMVGAPLDGVEIKLGMDG
                     EVLVRGPNVFSGYEGDPDLTAEAIDEDGFFHTGDVGRLTGDHRLEIIDRLKDVIITAG
                     GRKVAPRAIEDRLRADPLISGAMVFGEGRSYLTALISLDGQTASDIVETNPDNETGLW
                     EHPKVHQRVDRAVGTVNRSLSESEQVHGFAILPFGFPDEALTPTLKLKRRVVEETFAD
                     LIEGMYA"
BASE COUNT         1889 a         3145 c         3074 g         1771 t
ORIGIN      
        1 ctcgccgacg agatcaacag ggctccggcc aaggttcaat cggccctcct cgaggccatg
       61 ggtgagcgtc aaatctcaat cggcggtacc acctaccgac tcccgcttcc tttcctggtt
      121 ctggccacgc agaacccaat cgagtcagag ggcgtctacc cccttccgga ggcgcagcga
      181 gatcgtttcc tgatgagaat cccggtggac tatccgaccg cggtcgaaga gcacgagatc
      241 gtcgaccggt cccgccgcca gactgcagct cccgcctctc tgctctccac cgatgacgtg
      301 gcgagccttc agacggcggc ggaatccgtg tacgtcgatc gttccgtgca ggactatgca
      361 atccggctcg tcctggccac ccggaatccc tccgagcatg gagtcaagat cgacggcctc
      421 ctcgcctacg gggcgagccc tcgggcgagc atcggcctgg tggccgccgg cagggccctg
      481 gctctgctga ggggacgaaa ccacctcctc ccacaggatg tctacgacat tgcttacgac
      541 gtgctcaacc atcggctcgt cctttccttc gatgccgtgg ctgatggggt cactgtcgac
      601 gaggtgctga tcgaattgct caccactgtc gtagcgccgc gcctctcgtc atcacagctg
      661 atcgaacagc gatcacctgt gggggtctga gcatgctggg aagatccggt ccgccctctg
      721 gcgtggaggc gattccgcga gtgtcacggc tcacatcgcc gtcgcgggtg ccgatcgacc
      781 gcctccggat gactgtgctg aggcgcctgg acggcctgct ttccggtgat catgccggcc
      841 tcttccccgg tcatggcaca gagcgcggcg aagcccgccc ctatgtcccc ggagacgacc
      901 ctcgccacat cgactgggcg gttactgccc gcacccatga tccccatgtc cgggacacca
      961 tcgccgacca cgaactcgag gtgtggctcg tcctggacac ctcatcaagt cttggctttg
     1021 gaacgggcct cgccaccaag cacgaggtgg cctggtcagc agcgggggcg tttgccctcc
     1081 tcgcgtcctc gggcggcaac cgggtcggtg cagtccgctc caccaccgat ggcccggcat
     1141 cccgtcccca aatccttccg gcgcgcagtg gctcgagcca caccggcgcc gtgctcgccg
     1201 ccctctatcg ggctccggtc gatggcgagg caggcgacct cggcgctgcg atagacatgg
     1261 tccgccggac cgcccgtcgc cgcggcctcg ccgtggtgat ctccgacttt ttgggggagc
     1321 cgacatggga acgctcgtta cgagccttgt cacatcgcca cgagctgatc ggggtcgaag
     1381 tagtcgatcc acgtgaggtc gacctgcctg acgtcgggct gatctcggtc gtggatccag
     1441 agaccggtcg ccgtcgcctg gtcgacacct ccacctcgtc ggtccgccgt gcttacgggg
     1501 agttggctgt ccgccgccga gccgacattg ccgctcgcct caagagttgt ggcgccgatc
     1561 aactgacgct caggacggac cgcgactggg ttctcgattt tgtccgattc gtgtccgggc
     1621 gcaaagcccg ccttatggcc gcagggaggt ccacacgttg agcgacttcg gattcctcgt
     1681 acccggccga ttggcccttc tggtgctccc catcgccatg ggccttgcct acctggtgct
     1741 ccaggcgaga cgacgccgct atgcgatgcg tttcacgacc atcgagatgc tcgaccaggt
     1801 cgctcccgac cgcccaggat ggcgccgcca tctcaccgcc ctcggcctcg tgctcgcggt
     1861 agtcgtggcg accgtggcgt tcgcccgccc ggtgatcgcg ggggagacct atgagtcggt
     1921 ccagactgtt gtgctggcca tcgacacgtc actctccatg gaggccgacg acgtgtcgcc
     1981 atcacggatc gacgcagtcc gggatgctgc cggggagttt ctcgacaccg ttccggaggg
     2041 tgtttcagtc ggcgttgtcg ctttcgatgg cgaggcacgc gagctgatcg ctccgacaga
     2101 gcgcctcgac gccgttcgcc gggtcatcga caagtcgatc gatcacggcg accttgggga
     2161 gggaaccgcc atcggagagg cggtgttcgc cgggatcaac gccatcgagt cgtcagcacc
     2221 ggacctggag gcgaccacac cagaaacagg tgagtcggtc ggaaccatcg tggtgctttc
     2281 cgacggtgag accacgatgg gtagaaccaa cgacgaggcc gccaccgcag ctcgcaacgc
     2341 gagtatcccg gtccatacga ttgctttcgg cacggacgca ggcaccatcg ttgacccgta
     2401 cggcgaggtt gttccggtcc cggtcaacga ggccgcactt cggaaattgg cggattagac
     2461 agacgggaag gcactcaccg cggccaccgc ggagcaactg ggccaggtgt acgaggatct
     2521 cggccgctcg gttcaagtcg agaccgagcc agtcgagatc gccgattggt tcatcggagc
     2581 ggcactggcg gttctcagcc tggccggtct gggctcactg gtctggttcg gccgacttcc
     2641 ctgaaaggac cgaactcagg cggccactcg atacagccgc caacggtcgg ggatgcctct
     2701 caattcgtgc tcgccggcct ctcggaagtg gagatcacca ccggccgtca gatccttgac
     2761 ggtctgggag accaagatct ccgaggcccc ggcctgaccc atgacccggg caccgatcga
     2821 cactgcgaga ccggcgcact tcccgtcagc gatctcgcac tcaccagtgt gcaccccgac
     2881 ccgaatctgg acacctaggg gtgccaggtc ggtcatgaca tctcgggcgc actcaatggc
     2941 gcgggccggc ccgtcgaaca cgatgtagaa gccgtccccg gccgtgtcct gctcccgccc
     3001 tccgtggcgc tccaattggc gacgcacgat ccgatggtga gcctccagga gccgtttcca
     3061 ctcgtggtcg ccgaggcttg cctgaagtga ggtcgagtcg atgatgtcag tgaacatgac
     3121 tgtggccagg attcggtcgg tcctaggcac ctgccgcttg ccggtgacga actcctcgac
     3181 cacgtcgagg atggcatcac tgtcacccgc gatcgggaga tgatctacgc cggggagctc
     3241 gacatatcga gcgccggcaa tctggtcggc gacaaagcgt gcctggtctc ggggcacgat
     3301 caggtcccct tcccgatgca agaccaaggt cgggacctgg atggcgggga gcacatcccg
     3361 aatatccaga tcggtcaata tctggcccag ctctcgatag gccgccgggg tagccaccag
     3421 ccgctcgaag cgggcccacc acttgacgaa tcgctcgtcg tgcgccagcg acggcgcctg
     3481 aatcgagagg ttcttcccgg tacccacctc tctgacaaag cgatcgagaa aggatgcctg
     3541 ctcttcggcg ctgatcccta cagaaaagtc cgacgacgcc accctcttgg cggtcggggc
     3601 gtagaggatc aagccttcgg tgcgctgggg gtgggtggcg gcaaaaagcg tcgacatgct
     3661 cgccgctctg gcgacaccga agatgatcgc atcctccacg cccacggcgt ccatgactgc
     3721 acccaggtcg tcgcgctgtc gctcgaggat gggcgcctct gatccccggt ccgactggcc
     3781 gattcctcgc atgtcgaaca cggtgagacg ggagaaggat gccattcggc caaggaggtg
     3841 ctcccaatgc ggctcctccc acatcacatc tacgttggaa aagagccccg aacagaagat
     3901 caggtcgggt ccgttgccga tcgtctgata ggcaatagag atgtcgccac tcttcgcgta
     3961 tcgggtgtcg ggaaagtcag tcacggagac cggcacacgc taacgctgac cgtccccgga
     4021 atctgtcaca ggcgcccggc acactcttgg tcacggcgat tcctgaagtt tcttgtcggc
     4081 ccctacgggg ccgacaaccc ggggggaggg gttgacagca ctgccagcca tgccgtattg
     4141 gtgcggcccg ttcggtcgca aggggagtgg gaagtgccca tgaaacagaa ggaggaccaa
     4201 tgaaaccagt acatatttcg atcgttctgg accgctctgg atcgatgtcg aggatcgccg
     4261 acgatgtcgt cggcggcttc aaccagttcc tgcgcaagca gcgggaaacg gaaggcgaag
     4321 cgagggtgac cctcgtccag ttcgactccg acgatccgtt cgaactgctc atcgacggag
     4381 tgccgttacg ggaggtcatc gacctcgacc gaactggtta tcaacctcgt tccacgacgc
     4441 cgctctacga cgcggtgggc cggatgatcg tccgcattga cggtgtgatc gtcagccgca
     4501 acgagcgcgc actacccgag gaagaccagg tggtcctgat cgtcaccgac ggtttggaga
     4561 acgccagcac cgatttcgac cgctcgaagg tcttcgagat ggtgtccgac cgtcgcaagc
     4621 agggttgggt gttcgtgttc ctcggcgccg accaggacgt ctacgccgaa ggaggcaagg
     4681 tcggagtagc cacggccaat cgggttgctt gggagaagtc gaaggcgggc agcgacaaga
     4741 tgtggaagga catcgcctac tcgaccacca cttatcggga gaaagaagag ttcctgcgcc
     4801 gtcaggatcg agaccgcttc catgaggagg acccggacaa ggagtgacct tcttgtcgtc
     4861 cacccgcgca acgcttctat gcgtagaact cctccgcgat gtagatcacg cgaccacctt
     4921 cgatccggat ccagaccgcg caggttccgg tcaccgagca ccattcggct ccccagtcgc
     4981 ggtacaaggt gccagcgaac gactcggact cgaactctga aacgatcatc tgtgtgacaa
     5041 ggaggtcccc ctcgacgggc agagtccgca aggcagcgct ggaattgacc atttcgacgt
     5101 cgacccagtc attggggagg aggcaagcca ggtcgaagtc gatcgcgtca ctgcgtcggg
     5161 cggcgatgta accgaaccag atgccatcgg tgagcggacc cggcccaggg ctgcatcccg
     5221 agccataggc accatcggat gcgaggtcgt cgatgccctg agccctgatg agcagatcct
     5281 cgaagagttc agagccggag tccaagtcca actcgacccg gtacatctgt ttgtcggccg
     5341 agccaaatga gcacccggag tagaactcgt tctgctcaca agacccaagt tccaactcgg
     5401 gcagatcgga ctcgacgaag acgcggaaga gttcgcgttt ctggtccgag acgaaggcgt
     5461 aactgtccag tagcccaaag ttgtcctggt tggcagcgtc gacaagagcc tgggcccacg
     5521 actctggata gtccgacacg gtgatggatt ccggtgcggt cgtggatgtt gtaggtgtct
     5581 cgatggttgt cgtactgatt gcgatttcgg tgctctcggt cacctcttct ttgatcgtcg
     5641 tcgtggttgg gaccgcctgc tttcctcccg tcgttgtagt cgcggcggat gtcgggggag
     5701 ttgcgcaggc caccagaagc aacgtgacca gcgaaaagag aagagactca gatttcccca
     5761 accagcgaag tgttgccaca cgctgatcat gatcgaaagg cgtcacagcg cctatggggc
     5821 atcaccccta tgggatcggg ggcactcgag ccggctttct acactcgccg catggaaccg
     5881 gagacgaggt atgcgcgcct gggcgaggat cgcatcgcct atcaggtggt cggggacggt
     5941 ccggttgacc tggtgatcag caggggcacc ttctcggtcc ttgatgcgat ttggggaacg
     6001 tcggagaccg cggcccccta cctccagctc gccgcggtgt gccggttgat ccttttcgac
     6061 cgactgggca gcggctggtc ggacgccgtc ccgctcgaca cgttgccacc actggagagc
     6121 cggtgggcgg aatctgtgcg gtcatggatg cggctgagag tcaacgggcg gtcatcatgg
     6181 gggtccagga cggcgggccg tcggtcatgt tcggtgcggc aacccaaccc gatcgagtgg
     6241 ctggcctgat cctgacccac tccccggctc ggagtcgggt tgatgtcgac tatccgttcg
     6301 ggtacgacga ggcgaccatc gccgaacagt cgaagatggt tgcggagtgg gacctcgaca
     6361 ccatgatctc cagatccttt cccagtcggg gaaacgatga gcggttcttg cagtggggcc
     6421 ggagatacat gagggggatg gcctcgccga ctgccatgcg ggcctatacc gaagagatgg
     6481 gtcaaatcga cgtgcgcgac ctgcttccgt cgattcgggt gcccactctg gttcttcatc
     6541 gccgagaatt cgcctgggct ccacccgagc tcagtcgcta cgtggccgat cacatcgctg
     6601 gcgccaggta cgtcgagttg cccggaggag acgccgactt cttctttgat gagaccgccc
     6661 cgatgctcga tgcgatcacc gggttcctgg ccgagatcga gccccgcacc atccagcgcc
     6721 gttccgcgga gcgaatgatg gcaaccatcc tgttcaccga catcgtctcc tcgacagagc
     6781 gggctcaggc gagcggcgac gcgaagtggg tggtccaact ccagctccat gacgacatgt
     6841 ccagggatct gatcgggaag catggcggtc gtctggtcaa gagcaccggt gatggggtgc
     6901 ttgccctttt cgatggcccc gggcgaggcc tattggccgc cggagacatg tgctcgtccc
     6961 tggatcgggc cggcatgccg gtacgggccg gcctccatgc gggcgagatc gaactaaggg
     7021 ggcgaggatg tggccggggt cggggttcac atcgcagcgc gggtgatggc cgaagccgca
     7081 ccggcagagg tgttggtctc caggacagtt cgcgatctcg tggtcggctc cgactttcga
     7141 ttcgaggatc ggggctcgca ccttctcaaa ggtgtggacg gcgagtggca gctgttcgcc
     7201 ctagccgggc actgataatt ggcaggcccg acgtgaggaa cctgcctgtt tacaaggcgt
     7261 agaactcctc gaggtcccag tactgctcgg ctgggtcgag atcaggcctc tcgaatcggc
     7321 cccagatgaa gaccaggaga gccagcacca ggccggcggc cccaaaccac agcggccttg
     7381 ttagccacca ttgcacgctt cccatctcgg gctgggggaa cccgagggga agcaggatcg
     7441 atgcggcgat caccagggca gacaggtgcc acaggaaaag ggtcatgatc gaagagttga
     7501 tcgcgatcac aacgctccag gggcgctttc gagccagcca tttggtgaga gaaggacgga
     7561 gcaacattga caggccgacc tgatagaccc caaagaccat gatcgccagg gtcgggggtg
     7621 ccatgttcga gatttcatca ctgcaacaac cgaccatgct cgccggatag acctgcattt
     7681 gggtcagccc caccatcgct gcgaacccac cgatagccat gatccagaac aagctcctcg
     7741 agacccggtc aaaagtcccg tccgcataga agaacccgaa ttgatggatg aagagccata
     7801 cgaataggaa gttgaaccac ccaatcactg gtacatcgcc tgcgaatcgg agcagatcaa
     7861 tggcgaccgg gatcagaacg agtgccacca ggacccggat gcggaacttg cgatgcagga
     7921 agaccatcac cggcgccatc agcaccagca tgaggtaggc gcagataaac cagagtggcc
     7981 ccagtagaac aaggacagcc ttctctgcag ccccatccgg caggtcgagt tggagggcga
     8041 atggggcgac gatcgcccaa aacaggagaa agatgcccac cggcctgagt aagcgactcg
     8101 cccttccttt gaggaagacg ccggggcctt ccccgagccg ctgtagtccc tcataggtca
     8161 cgaagttgga gaacccgccg acgaagaaaa agatcggcat gatttgcaag gcccaggtga
     8221 tgatccagag cccgggcaca acatcgaggg cgctggtagc ggtgagctcg ccatcctgca
     8281 gatagaccac ggctccgagc caatgaccca ggaccacgac cacaaccgat agagcgcgga
     8341 ggaagtcgac gtaacgatcg cgtttgtccg gtgtggcgtc ggccagatca gctagacggc
     8401 ggcgaccagg aggagtgcct ccgctgccgt cctgttgcat ggtgtcctca tgtttgcctg
     8461 atggcgcctt gccgtccact agccactcag ggaaccacca ccggtcgaag cggtggatcg
     8521 cccccatgcg ccgacccctg cgagataggt gaagctgttg attgcagcgt gaccaatcca
     8581 tggagcgacc agactcccgg atcgtcgacg catccagccg aacccgagac ttgaaatgcc
     8641 cgtcagcaat gccccagcca tcaccacacc tgcttccgcg caccgtgtcc cgatgcgggc
     8701 accgagcgga tttccccgga gcgcctggcc agatgggatg agatgccaaa gaccaaaggc
     8761 agtcgccgtg acaaccgcgg cggtccgtgg tgaggcaccc gagtggcgcc acgtcccgta
     8821 aatcacaccg cggaacgcca cttcttcgaa taatgccgtg cccaatggga acctcacggc
     8881 ggcccggtat gtgatctccg ccgcactgtg gccagccgcg cgctcatcca acaagtaggg
     8941 gcgtgctgag cgaagggtcg tgatcccacc cgccactacg gcggcacctg cagcccccgc
     9001 caggccgtgg cggattcccc tcccggcttc gagcggattc agaccgagct cgtcccaggt
     9061 gcagccattt ctccgcgcca gggccaccaa ccccagcgtg gcgaccatgt tcgcggggac
     9121 atacgcgcgc tctgaaacca tgtagttctg gaccaggttg tatccgccga cagcccccaa
     9181 gaccaagaac cgaccccgat caggcataca tcccttcgat caggtcggcg aaagtctctt
     9241 cgacaacccg acgcttcagc ttcaaggtcg gggtcagggc ttcgtcgggg aacccgaaag
     9301 gaaggattgc gaatccatgc acctgttcgg actcggacag ggaccggttc accgtcccga
     9361 ccgccctgtc aactcgctgg tgcaccttcg ggtgttccca caatccggtc tcgttgtccg
     9421 ggtttgtctc gacgatgtcg gaggcagttt gcccgtcgag cgagatcagg gctgtcaggt
     9481 aggagcgacc ctctccgaac accatggctc ccgaaatcag gggatcggcc cggagtcggt
     9541 cctcgatcgc cctcggggcg acctttcgcc caccggccgt gatgatcacg tccttcagcc
     9601 gatcgatgat ctcaagtcga tgatctccgg tcagccggcc cacatcgcct gtgtgaaaga
     9661 agccgtcctc gtcgatcgcc tcggccgtta gatctggatc gccctcatag ccggaaaaga
     9721 cattggggcc acggaccagc acttcgccgt ccatcccaag tttgatttcg accccgtcca
     9781 gaggagctcc caccatgccg atctcgaggt cgtcaggtcg ctggacggca cacagtgctg
     9841 ccgtctcggt gagaccccaa ccctgatgga gtccaagac
//
LOCUS       DBNHXU010000016         6514 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1393658, whole genome shotgun sequence.
ACCESSION   DBNHXU010000016 DBNHXU010000000
VERSION     DBNHXU010000016.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6514)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6514)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6514
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1393658"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..132)
                     /locus_tag="AC161X_00465"
     CDS             complement(<1..132)
                     /locus_tag="AC161X_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020631694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR4988050.1"
                     /translation="MVWLTIGELAERAGVATSALRFYEDRGLIASERTPGNQRRYPRA
                     "
     gene            187..1914
                     /locus_tag="AC161X_00470"
     CDS             187..1914
                     /locus_tag="AC161X_00470"
                     /inference="COORDINATES: protein motif:HMM:NF025178.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR4988051.1"
                     /translation="MTTQQGEWVAAIGIDDLAVGGRKLFRHYDKRIAIFRTEQAVYAV
                     DNRCPHQGYALLQGEMRGETLTCAWHNWKFELDENGTCSFGGEAVRAYPVRVNQGQVF
                     VDVTDPSAEVIAPQLFQSLLEAMGDVDIGRMARDTLRIRQIGRPLADVVREGVMYGAP
                     RMEYGWNHSLATLTDCLHLAAMFEGELQAFPVIQGMSVVAQNEVRRPFRPRPDPIDPV
                     ELYGSIDDALAAYPVLVDEERGDEAEGLLRGLIAVGVDKARIRHALLSAITDHFLGYG
                     HPMIYAQKSFEMLETVGWGEADTVLGPLVPSTVLATRYDRLPYMRKFSRAFQELAPDL
                     GRQATAGGHASFDARRFTLAVLDADAETACRALSEALDSGVAVLLLLDAISRAAAERL
                     RRFDIDIDLDDTNEWGWLDVTHNLTYLDALRWAWSVDSSPEVLRGLYHAVWFVNFTRQ
                     LDERAPRTRPEPWVNSDTGIVLDAIRAKDPEGVEAAIAGFQGPQEDLRGALAHGASED
                     NSVAPIMVAHAVKTARASIVESEIIGDRAPMIAAGRFLASPKRERFVYNATLEAIDFT
                     RGRARGEAS"
     gene            1933..2742
                     /locus_tag="AC161X_00475"
     CDS             1933..2742
                     /locus_tag="AC161X_00475"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR4988052.1"
                     /translation="MFDELAEGVYRRRFESLDLNVGLVFGESAVLVIDTRASHREADE
                     LIREIEAFTKLPVAWLVNTHWHWDHAFGNSRFPGAEIWGHRLCREALIDLGDEMLEEV
                     ADMFREDGNDAMADDIKEVVVVPPGSVFEDSVTIDLGGRAVELTYHGLAHTDADIVIR
                     IPDCGVTFFGDMLENGAPPNFGDSYPLSWPSTLGAAMSSLDGLIVPGHGEVMTPADAG
                     TQLEELKEVARAATWCLVDKHPVEEMAQTGPYPAEVMMAALNRALEVGGSG"
     gene            complement(2734..4728)
                     /locus_tag="AC161X_00480"
     CDS             complement(2734..4728)
                     /locus_tag="AC161X_00480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004984053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HIR4988053.1"
                     /translation="MASRYGHPVANRLAQASSPYLLQHQDNPVDWQEWGEEAFAEARE
                     RDVPVLLSVGYSACHWCHVMAHESFEDPETAEVMNRLFVNVKVDREERPDVDAIYMEA
                     VQAMTGRGGWPMTVWMTPDGQPFYTGTYFPNRDHHGMPSFSKVMTAVSDAWVNRRDEV
                     LEQSARLTEAINRDIPPADGLPQEPALQDAYETIAATFDPINGGFGEAPKFPQQPVLE
                     FLLRVHGEPWASRADSMLRTTLMEMAAGGIHDQLAGGFARYSVDNQWLVPHFEKMLYD
                     NAQLARIYLWAGKELADPGLLDVARATLDYLLTDLRHPDGGFFSAEDADSEGVEGKFY
                     VWTLGELASVLGDDDAAEAARFFGTTPHGNFEGSNILYRPTTDPWSARIDSIRQRLLE
                     ARSERIRPGLDDKVVTSWNGLALRALAEAGAALHDPGYLEAAESTGRFLLDHLIVDGA
                     LMRSWREGQTRVRGFLEDHASLALGLFALYAATGGYEWYAAAERLTRQIPLHFADPSG
                     GFYDNAEDSSLIKRPKSLADNPLPSGNAMAAEALAILAAYTGEAELRDLAISAITAAG
                     TLIERYPSMVGHHLSVLHGLGMARELAVVGPRPGPFESVYWERFRPAVVLATALEADD
                     RVPLLEGRGQPDSTLAYVCENQVCMLPTTDPEVVRAELSA"
     gene            4753..5406
                     /locus_tag="AC161X_00485"
     CDS             4753..5406
                     /locus_tag="AC161X_00485"
                     /inference="COORDINATES: protein motif:HMM:NF025279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HIR4988054.1"
                     /translation="MPDQRLEELYQSAEKRWLQNWLDGPGLPGNPLRRGDFAPDVQLV
                     GEDGSPVALSSYWSKRTALVMLWRHLGCGCGLERTTQLQKDYPDYVNAGLEVVVVAPG
                     DPQRVAFYKEKYGIPAPMLADGDYSTHRAFGLGHWSPEQVLYDAPDHFCELAEETGVD
                     FQAERRAMGRPLVDDPWMQAGEFVIGVEGVIRVAYPYNYCEDFPDPRIFTTAARLAG"
     gene            complement(5403..6038)
                     /locus_tag="AC161X_00490"
     CDS             complement(5403..6038)
                     /locus_tag="AC161X_00490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012888242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HIR4988055.1"
                     /translation="MPEPSTLLVFLGATLLLLVIPGPAVLYIVTRSVTQGRRAGFVSV
                     LGIHLGSLVHVAAAVVGLSALIAASATAFAVVKFAGAGYLIYLGIMAFRSAGRPEGLA
                     RPEPMSMRRIFWQGAIVNILNPKTALYFLAFVPQFVDPALGNTTLQLIVLGGIFIVAG
                     LISDGTYAMVAGAAGARFLDSDAWRRGQGYVAGTIYTGLGLAAAFTGGSKA"
     gene            6096..>6514
                     /locus_tag="AC161X_00495"
     CDS             6096..>6514
                     /locus_tag="AC161X_00495"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR4988056.1"
                     /translation="MKTILLMRHAKSDWDASYASDHDRPLAPRGHRSATVMGRVLTAW
                     DEVPDLVITSSAARARATAEEVVRAGSWKCPIAVESDLYGTGPGTVLGVAEGTATDEE
                     RIMLVGHEPTWSMLAEQITGGRVEMRTAGVAAIDLLA"
BASE COUNT         1201 a         2025 c         2135 g         1153 t
ORIGIN      
        1 ggctctcggg tagcgccgct gattgccggg agtccgttct gaggcaatca agccgcgatc
       61 ctcatagaaa cgaagtgccg aggtggcgac tcccgcgcgc tccgccaatt caccgatcgt
      121 cagccacacc atctcggcag tctacctctt gacttcaagt gaacttgaag ttgtagggtg
      181 aacgacatga caactcagca aggggaatgg gtggcggcga tcggaatcga cgacctggcg
      241 gttggcggtc gcaagctgtt tcgccactac gacaagcgga tcgccatctt ccgcactgaa
      301 caggcggtct acgccgtcga caatcgctgc ccgcaccagg gctatgcctt gctgcagggt
      361 gaaatgcggg gcgagaccct gacctgcgcc tggcacaact ggaagttcga gctcgacgag
      421 aacggaacat gcagcttcgg gggagaagcg gtccgcgcct atccggtacg tgtcaaccaa
      481 ggccaggtct tcgtcgacgt gaccgatccc agcgccgagg tcatcgcacc ccagctcttc
      541 cagagtctct tggaagcgat gggtgatgtc gacatcggca ggatggcccg ggacacgttg
      601 cgcatccgtc agatcggacg accccttgcg gacgttgttc gggaaggggt gatgtacggg
      661 gcacctcgca tggaatatgg atggaaccac tcgctggcga ccctcaccga ctgtcttcat
      721 ctcgctgcca tgttcgaggg cgagctccag gcgttccctg tgatccaggg gatgtcagtg
      781 gtggcccaga acgaggtccg ccggcccttc cgaccccggc cggacccgat cgatccggtg
      841 gagctttacg gcagcatcga cgatgcattg gccgcctatc cagttctggt ggacgaggaa
      901 aggggggacg aggcggaggg gttgttgcgg ggtctgattg ctgtaggtgt ggacaaggcg
      961 aggatccgcc atgccctgct ctccgccatc acggaccact tcctcggata cgggcatccg
     1021 atgatctacg cccagaaatc gttcgagatg ctggagaccg ttggctgggg agaggctgac
     1081 acggtcctcg ggccactcgt gccgagcact gtcctcgcca ctcgctacga ccgtctcccc
     1141 tacatgcgaa agttcagtcg tgcgttccag gagctcgccc cggacctggg ccgccaggcg
     1201 actgccggcg gacacgccag ctttgatgcg aggcgtttca cattggccgt cctcgacgcc
     1261 gatgcggaga ccgcgtgccg ggccctcagc gaagccctgg attccggcgt cgccgttctc
     1321 ctgctcctcg acgccatctc gcgtgccgcc gcggagcgac tgcgtcgatt cgacatcgat
     1381 atcgatctgg atgacacaaa tgagtgggga tggctcgacg tgactcacaa cctgacgtat
     1441 ctcgatgccc tgcgctgggc ctggtccgtc gactccagcc cggaggttct tcgtggcctt
     1501 taccacgcgg tctggttcgt caatttcacc cgtcaactcg acgagcgagc cccgaggacc
     1561 cggcccgagc catgggtcaa ttccgatact gggatcgtcc tcgatgcgat ccgggccaag
     1621 gatcccgagg gagtcgaggc tgccatcgcc gggtttcagg gcccacagga ggacttgagg
     1681 ggtgccctcg cccacggcgc ctccgaggac aacagtgtgg ctccaatcat ggtggcccac
     1741 gccgtgaaaa ccgctcgtgc ttcgatcgtg gagtccgaga tcattgggga tcgtgcgccg
     1801 atgatcgctg ccggcaggtt cctcgcctct ccaaaaaggg agcgcttcgt gtacaacgcc
     1861 actttggagg caatcgactt caccagagga cgggctcgag gcgaagccag ctagtcggtc
     1921 tagcctcgcc tggtgttcga cgagcttgcc gaaggggttt ataggagacg tttcgagtct
     1981 ctcgatctga acgtcggcct ggtgttcgga gagtcggcgg ttctggtcat cgatacccga
     2041 gcctcgcacc gagaggccga cgagttgatc cgcgagatcg aggccttcac caagttgccg
     2101 gtcgcttggc tggtcaacac ccattggcat tgggaccacg cctttggcaa ctcccggttc
     2161 cctggcgccg agatctgggg ccaccgactt tgcagggagg cgctcatcga cctgggcgac
     2221 gagatgctcg aggaggtcgc cgacatgttc cgcgaagacg gcaacgatgc aatggccgac
     2281 gacatcaaag aggtggtcgt ggttccaccg ggatcggtct tcgaagactc agtgaccatc
     2341 gatctcggcg gccgggccgt ggagctgacc tatcacggac tggctcacac tgacgccgac
     2401 atcgtgatcc ggattccaga ctgcggcgtg accttcttcg gggacatgtt ggagaacgga
     2461 gcgcccccaa acttcggtga cagctatccc ctgtcctggc cgagcactct cggcgccgcc
     2521 atgtcatcac tcgacgggct gatagtcccc ggtcacggtg aggtcatgac gcctgccgac
     2581 gctgggaccc agctggagga gttgaaggag gtcgcccgcg ccgcgacctg gtgcctggtg
     2641 gataagcacc ccgtcgagga gatggcacag accgggccat atccggccga ggtgatgatg
     2701 gccgcgctca atcgggcact ggaagtcggc ggctcaggct gagagctcgg ctcgcaccac
     2761 ttctgggtcg gtggtgggca gcatgcagac ctggttctcg cacacatagg cgagggtcga
     2821 atcgggctgc cctcggccct ccagaagcgg cacccggtca tcagcctcca aagcggtggc
     2881 gagcaccaca gcggggcgga accgctccca atacacactc tcaaatggac cgggccgggg
     2941 acccacgacc gccaattcac gggccatccc caggccgtgg aggaccgaga gatgatgccc
     3001 gaccatcgac ggatagcgtt cgatcagtgt tcctgctgca gtgatggcgc ttatcgccag
     3061 gtctctgagc tcggcttcac cggtgtatgc cgccaggatg gccagcgctt cggccgccat
     3121 cgcgttcccc gaagggagcg ggttgtcggc caggctcttg ggtcgcttga tcaagctgga
     3181 gtcttcggcg ttgtcgtaga acccgcctga cgggtctgcg aagtggaggg ggatctgcct
     3241 ggtgagccgc tccgccgccg cgtaccactc gtagcccccg gtcgccgcgt agagagcgaa
     3301 aaggccaagc gccaacgagg catgatcttc gagaaacccc cggacccggg tctgaccttc
     3361 ccgccaggac cgcatcaggg cgccatcgac gatcaagtgg tccaggagga agcggccggt
     3421 ggattcggcg gcctccagat agccgggatc gtgaagggcg gcgcccgctt cagccagggc
     3481 ccggagcgcc agcccgttcc atgaggtgac caccttgtcg tcgaggccgg ggcggatccg
     3541 ctcgctgcgt gcctcaagga gacgctgtct gatcgagtcg atgcgggcgc tccaggggtc
     3601 ggtggtgggc cggtaaagga tgttggaacc ttcgaaattt ccgtgtgggg ttgtaccgaa
     3661 gaagcgtgcc gcctctgcgg cgtcgtcgtc ccccaggacg gaggccaact cacccaacgt
     3721 ccacacatag aacttgcctt ctacgccttc ggagtccgca tcctcggccg agaagaaccc
     3781 gccatcaggg tggcggaggt cggtgagcag atagtcgagc gtcgcacgag caacatcgag
     3841 cagacccgga tccgccagtt ccttgcccgc ccagaggtag atgcgggcga gctgtgcgtt
     3901 gtcgtagagc atcttctcga aatgaggaac cagccactgg ttgtcgaccg agtagcgggc
     3961 gaagccccct gcgagctgat catggatccc acccgctgcc atctccatca gggtggtgcg
     4021 aagcatggag tcggcccgcg atgcccaggg ctcgccgtga acccgcaaca agaattccag
     4081 gaccggctgc tgagggaact ttggcgcctc accgaaccca ccgttgatcg ggtcgaaagt
     4141 cgccgcaatc gtctcgtatg cgtcctgcaa cgccggctcc tggggcagac cgtcggccgg
     4201 aggaatgtcc cgattgatgg cctcggtcaa tcgcgccgac tgctcgagga cctcgtcacg
     4261 ccggttcacc caggcatcgg agaccgcagt catcaccttg gaaaacgaag gcattccgtg
     4321 atggtctcgg ttggggaaat aggtacccgt gtagaagggc tgaccgtcag gggtcatcca
     4381 gacggtcatc ggccacccac ctcgtccggt catcgcctgg acggcttcca tgtagatggc
     4441 gtcgacatcc ggtcgctctt cacggtcgac tttcacgttg acgaaaaggc gattcatgac
     4501 ctcggccgtt tccgggtctt cgaatgactc gtgtgccatc acgtggcacc agtggcaagc
     4561 cgaataaccg acactgagca gaacgggcac gtcacgctcc cgggcctcgg cgaaggcttc
     4621 ctcgccccat tcctgccaat cgacggggtt gtcctggtgt tggagcaggt aaggagagga
     4681 tgcctgggca agacggttgg ccacaggatg accgtaccgc gatgccatga ctggttggct
     4741 aagtttcgac acgtgccaga tcagcgtctc gaggagctct atcagtcggc ggagaagcgc
     4801 tggctccaga actggctcga cggccccggg ctgcctggca acccgcttcg acgtggcgat
     4861 ttcgccccag acgtgcaact ggtgggagaa gacggttcac cggtggcctt gagctcgtac
     4921 tggtcgaagc ggacggccct ggtcatgctg tggcgacacc tcggttgtgg gtgcgggctg
     4981 gaacggacga cccaactcca gaaggactac cccgactatg tcaacgctgg tctcgaggtg
     5041 gtcgtggtgg ccccgggcga cccgcagcgg gtcgccttct acaaggagaa gtacgggatc
     5101 ccggcgccga tgctggccga tggcgactat tccacacacc gggcgttcgg gcttggccat
     5161 tggtcacccg agcaggtcct ctacgacgct ccggaccact tttgcgagct agccgaggag
     5221 accggtgtcg acttccaagc cgaacggcgc gccatgggcc ggccattggt cgacgaccca
     5281 tggatgcagg ctggggagtt cgttatcggt gtggaaggag tcatccgggt ggcttacccc
     5341 tacaactatt gcgaggactt tcccgatccc aggatcttca ccacggcggc ccggctcgcc
     5401 ggctaggcct tggatccacc ggtgaaggcg gccgccaggc cgaggccggt gtagatcgtt
     5461 cccgcgacgt aaccctgccc ccggcgccag gcgtcactgt cgaggaagcg ggctccggcg
     5521 gccccggcaa ccatcgcata agtcccgtcg ctgatcagcc cggcgactat gaagatcccg
     5581 ccgagcacga tcagttggag agtcgtgttc cccagcgccg ggtccacgaa ctgggggacg
     5641 aatgcgagga agtacaacgc cgtcttcggg ttgaggatgt ttacgattgc gccctgccag
     5701 aagatgcggc gcatcgacat cggttcgggt cgggcgaggc cctcgggcct gcccgcggag
     5761 cgaaaagcca tgatcccgag gtagatcagg tagccggcac cggcaaactt gacgactgcg
     5821 aatgcggtag ccgatgccgc gatcagggca ctcagcccga cgaccgcagc cgccacgtgg
     5881 accaggctcc cgaggtggat tccaaggacc gagacgaaac cggcgcgccg cccttgggtg
     5941 acgctgcggg tgacgatgta gagaacggcg gggccgggga tcacgagcaa caacagagtg
     6001 gcgccgagga agacgagcag ggtggagggc tcgggcatcc ggtgaggcta aacctcacgg
     6061 gcaccagtca atccccaatt tcgtaggctg acgcggtgaa gaccattctc ctcatgcgcc
     6121 acgccaaatc cgactgggat gcttcctacg cctccgacca cgaccgtccc cttgccccga
     6181 ggggccatcg gagcgcaacg gtgatgggca gggtgctcac agcttgggac gaggtcccgg
     6241 atcttgtgat cacgtcgtcc gcggctcggg cccgggcgac tgcagaagaa gttgtccgcg
     6301 ccgggtcgtg gaaatgcccg attgcagtcg agtccgatct ctatggcacc ggcccgggga
     6361 cagtcctcgg agtagcagag ggcaccgcca ccgacgagga gcgaatcatg ctcgtcggcc
     6421 atgagcccac gtggtcgatg ttggccgagc aaatcacggg cggccgggtg gagatgagaa
     6481 ccgccggggt ggctgccatc gaccttctgg cgga
//
LOCUS       DBNHXU010000017         4655 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3179568, whole genome shotgun sequence.
ACCESSION   DBNHXU010000017 DBNHXU010000000
VERSION     DBNHXU010000017.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4655)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4655)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4655
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3179568"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..341)
                     /locus_tag="AC161X_00500"
     CDS             complement(<1..341)
                     /locus_tag="AC161X_00500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019178943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR4988057.1"
                     /translation="MSVRGAASLGIGAMVGAGIFALLGEAGAVAGNATWLSFLLGGLV
                     ATLLGYVVAKLGARYPSKGGLVTFLMEGYGPGHLAGVFSWLLYFAAIIITAMVAVSFG
                     SYAVALFIGEGA"
     gene            complement(388..1728)
                     /locus_tag="AC161X_00505"
     CDS             complement(388..1728)
                     /locus_tag="AC161X_00505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic C4-dicarboxylate transporter family
                     protein"
                     /protein_id="HIR4988058.1"
                     /translation="MDITFILEGLVVLGAIVMGTRAGGVGIGLWGGLGVAILVFVFQE
                     PPGEPPISALLIILAVVTAASMMQVAGGVDWMVKVAAGVIQAHPKQITVIAPLTAFAF
                     TVGAGTSNIMYPLLPVIYDVSYQNNIRPSRPLTVAVVQSGMSLAASPVSAAMAAMLTL
                     TAVAPYNFDLAQILSITIPAGIIGIIVTALFVGRMGLDIDDDPEIQAKIASGELPKPG
                     ASGTKIELTATKEGRNAGIIFLLGVVAIVIFGLFPDLRPSFGAEGEAVPIDMTTTIEL
                     VMFSVGVLIMLVARPDVSQVPEASVFKAGMVSAIALFGIAWLTATFIAAHEEFIVTTI
                     GGWVNNATWIFALAIFLVAALTTSQSTATRTIVPIGLAAGLAPGLVTGMWGGALGGVY
                     TLPANGTQIAAANFDLTGSTKLGTKLLDHSFFVPMLIMTVVSVVVGALIGVIFY"
     gene            complement(1744..2160)
                     /locus_tag="AC161X_00510"
     CDS             complement(1744..2160)
                     /locus_tag="AC161X_00510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988059.1"
                     /translation="MTYLRQQKWAFALTALALILTACGNAQERDLCREYEDLQQAVTQ
                     VDELDPETATAADALAIVENVMLQLDQFQAVSDGLYDQAVSNLNLALEDLRVVVFDLG
                     DEGLVVARPLMEDSLTNTAAAYDALKQRLDVVCGTD"
     gene            complement(2177..3286)
                     /locus_tag="AC161X_00515"
     CDS             complement(2177..3286)
                     /locus_tag="AC161X_00515"
                     /inference="COORDINATES: protein motif:HMM:TIGR01883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR4988060.1"
                     /translation="MSGLADRVDQDRLLRTFLELVAIDSPTGHEEAIGRELTSRFEKL
                     GCSVTQDHVGNLVAVLPGDRPDTIMISTHMDTAGTDIGIIPIIEDGVIRTDGSTILGA
                     DDKSGIAGCLEVLTLLREDPTASHPTVEFVISVGEESGLVGSRQMDVSQLNATHGFVF
                     DTAGAISAITYWAPTSVYITVKVHGKKVHAGVEPEKGINAVKVAAEAIAAMPLGRIDE
                     ETVANIGTITGGEARNIVPGEVVLEGMARSHDQSKLDEQLDAMRTALEDAAARHGATV
                     DFEAEEIYRTYRIEESETPYREAARAIRSLGLEVVPRKSGGGTDGNFFNAKGIPCVAM
                     ATGMVDEHATTEHIAIEDMVTACKILVTIATQDPE"
     gene            complement(3283..3651)
                     /locus_tag="AC161X_00520"
     CDS             complement(3283..3651)
                     /locus_tag="AC161X_00520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR4988061.1"
                     /translation="MDLFWTMLWLFLFFAWIWLLITVFSDIFRSEDLGGWGKALWIMF
                     TVFLPYLGVLVYLIARGGSMQQRSLAEAAAREEMTRAYVQQVARSGSTAEELTQLANL
                     RDTGVITAEEFNQQKAKLLL"
     gene            3818..>4655
                     /locus_tag="AC161X_00525"
     CDS             3818..>4655
                     /locus_tag="AC161X_00525"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR4988062.1"
                     /translation="MRSRFATIALTVLLGLTISGCTEEEPLDVAAAQAQFCSDVEQYV
                     SAIGSYGGLFEDVDLTVGDVKNAADELAPGREAVEESAATFQEAVTADTTPGLTVEII
                     QPESIDAVKDAEAAFAAASDIDDNTTVVEAGVNFTSAAYGLEVAWVRLFADAGCIEDE
                     AQASQWVSDYVTALQTDLQAAGLYEGDIDGIYGPQTIAAVEQLQKDAGLPVTGLVDPA
                     TQAALAAALGQRQSAEIGALQGILISTGYYDGVVDGIWSREVEDALKALQTDLGVPAT
                     GVV"
BASE COUNT          939 a         1448 c         1427 g          841 t
ORIGIN      
        1 gcaccttcgc cgatgaacag ggcgaccgcg tagctcccga aggacacggc gaccatggcg
       61 gtgatgatga tggcggcgaa gtagaggagc catgagaaga cccctgccag gtgaccgggg
      121 ccgtaaccct ccatgaggaa cgtcaccaat cctccctttg acgggtagcg ggccccaagc
      181 ttggcaacga cgtatccgag cagcgtcgcc accaggcctc ccagcaagaa ggacagccac
      241 gtcgcattgc cggcgaccgc accggcctcc ccgagcaggg cgaagatgcc ggctccgacc
      301 atggcgccga tcccgaggga cgcagctccc cggacgctca tccggggagc cgttacgttc
      361 tcagacgttg tcatcagtct ctctcgttta gtagaagatc acgccaatca gggcaccaac
      421 cacgaccgag acaacggtca tgatgagcat cgggacgaag aagctgtggt ccaacagctt
      481 ggtccccaat ttggtcgacc ccgtcaggtc gaagttggct gccgcgattt gggtgccgtt
      541 ggcggggagg gtgtacacgc cgccaagcgc accaccccac ataccggtca ccaagccggg
      601 ggccaacccg gcggcaagac cgattgggac gatggtccgg gtcgcggtcg actggctggt
      661 tgtcaaagcc gcaaccagga agatggcgag ggcgaagatc caagtcgcat tgttgaccca
      721 gccgccgatc gtggtgacga tgaactcctc atgcgccgcg atgaaggtgg ccgtgagcca
      781 ggcgattccg aacagagcaa tcgccgagac cattcccgcc ttgaacaccg aggcctcggg
      841 cacttgtgac acgtcggggc gtgcaaccaa catgatcagg acgccgacgc tgaacatgac
      901 cagttcgatg gtggtcgtca tgtcgattgg gaccgcctca ccttcggcac caaatgaagg
      961 ccgcagatcg gggaacagac cgaagatgac gatggcgacc acgccgagca ggaagatgat
     1021 cccggcgttc cggccttcct tggttgccgt caactcgatc ttggtgccgg acgctccggg
     1081 cttgggcagt tcacctgaag cgatcttggc ctggatctct gggtcgtcat cgatgtcgag
     1141 gcccatccgc cccacgaaca gagcggtgac gatgatgccg atgatgccag ccgggatcgt
     1201 gatggaaagg atctgcgcca ggtcgaagtt gtagggggcg accgcggtga gagtcaacat
     1261 cgccgccatc gccgcggaga ccggtgacgc cgccagcgac atcccggact gcaccactgc
     1321 aacagtcagc ggtcgcgatg gcctgatgtt gttctggtag gacacgtcgt agatgaccgg
     1381 gagcagcgga tacatgatgt tgctggttcc cgccccgacc gtgaaggcga aggcggtgag
     1441 aggcgcgatg acggtgatct gcttggggtg agcttggatc acacctgcag ccactttgac
     1501 catccaatcg accccgcctg ccacctgcat catcgaagcc gcggtgacta ccgccaggat
     1561 gatcagcagc gccgagatcg gcggctcccc cggtggctcc tggaagacga acaccaggat
     1621 ggcgacaccg agaccgcccc agaggccgat cccgacgcca ccggcccggg tgcccatgac
     1681 gatggcacct aggaccacca agccttcgag gatgaaagtg atgtccattt gtctcctttg
     1741 gaatcagtcc gtgccgcaga cgacgtcgag tcgctgcttc aaggcgtcat atgccgcagc
     1801 cgtgttggtc aacgagtcct ccatcagcgg cctcgccacc acgaggcctt catcaccgag
     1861 atcgaaaacc accactctca gatcctccag ggcgagattc agattcgaga ctgcctggtc
     1921 gtaaagaccg tccgagactg cctggaactg atcgagttga agcatgacat tctcaactat
     1981 cgccagcgca tcggcagcag tggccgtttc cggatccagt tcgtcgacct gcgtgaccgc
     2041 ctgttgaaga tcctcgtatt cgcgacagag gtccctctcc tgggcattcc cacacgcggt
     2101 aaggatgaga gcgagcgcgg tgagcgcgaa cgcccacttc tgttgcctga gataggtcat
     2161 ggtgccgctc cctccttcac tccgggtcct gagtggcgat cgtcaccagg atcttgcacg
     2221 cggtgaccat atcttcaatg gcgatgtgct cggtcgtggc atgctcatcg accatcccgg
     2281 tagccatggc gacacagggt atgcctttgg cattgaagaa gttgccatcg gtccctccgc
     2341 cgctctttcg tggaaccacc tccagcccga gcgaacggat ggcccgggcg gcctcccggt
     2401 atggcgtttc cgattcctcg atgcgatagg tgcggtagat ctcctcggcc tcgaaatcaa
     2461 cggtcgctcc atgccgggcg gcggcgtctt cgagagcggt tcgcatcgca tctaactgtt
     2521 catcgagttt cgactgatcg tgactgcggg ccatcccctc taggaccact tcaccgggga
     2581 caatgtttcg cgcttcccca ccggtaatgg tcccgatatt ggcaacggtc tcctcgtcga
     2641 tgcggccgag cggcatggct gcgatcgcct cggcggcgac tttgaccgca ttgatgccct
     2701 tctcgggctc aacgccggca tgcaccttct tgccgtggac cttcaccgtt atgtagacgg
     2761 aggtgggtgc ccagtaggtg atcgcgctga tcgcccccgc ggtgtcgaac acaaacccgt
     2821 gggtggcatt gagttggctg acgtccatct gacgggagcc aacgagaccg ctttcctcgc
     2881 caacgctgat cacgaactcg acagtggggt gtgaagcggt gggatcctcc cggagcagcg
     2941 tcaagacctc gaggcaaccc gcaatacccg acttgtcatc ggccccgagg atggtcgagc
     3001 catcagtcct gataaccccg tcctcgatta tcggaatgat tccgatatcg gttcccgcgg
     3061 tgtccatgtg ggtcgagatc atgatcgtgt cgggacgatc tccgggcagg accgcgacga
     3121 gattccctac atggtcctga gtcacggagc aacccagctt ttcgaagcga gaggtcagtt
     3181 ctcgaccgat ggcttcctcg tggcccgtgg ggctgtcaat ggccaccagt tcgaggaaag
     3241 tgcgaagcag gcggtcctgg tcaacgcgat cggcgagtcc gctcaaagca gaagcttggc
     3301 cttttgctgg ttgaactcct cggcggtgat gaccccggtg tcccgcaggt tggcaagctg
     3361 ggtgagctct tcggccgtgg agccgctcct cgcgacctgc tggacatagg cccgggtcat
     3421 ctcttcacga gcagccgcct cggcaaggga cctctgttgc atcgagccgc cacgagcgat
     3481 gaggtatacg aggacgccta ggtagggcag aaacacagtg aacatgatcc acagcgcctt
     3541 gccccagccg ccgagatcct cactgcggaa gatgtcggag aacaccgtga tcagcagcca
     3601 gatccaggca aagaacagga acaaccagag catggtccag aacaagtcca tcaatggcac
     3661 gcttgtctcc tctaatggtg cgaggcccaa aagatacacg gttggcggag gggctcgtgc
     3721 cgcataggaa cccggattac tcagatagcg gtgacgtcga attagcttgg cttgaaacgt
     3781 ccacggccac tctccgagtc aaagtgaagg aactgtcatg agatcaagat tcgcgactat
     3841 cgctctgacc gtcctcctcg gactcacgat ctcgggatgc accgaggaag aaccgctcga
     3901 tgttgccgcc gcccaggcgc agttctgctc ggacgtcgag caatacgtgt ccgcaatcgg
     3961 tagctacggc ggcctgttcg aggatgtcga cctcacagtc ggtgatgtca agaatgccgc
     4021 agatgaactg gcacccggcc gcgaggcagt cgaggagtcg gcggccacct tccaggaggc
     4081 cgtcacggct gacaccacgc ccggtctcac ggtcgagatc atccagcccg aatcgatcga
     4141 tgcggtcaag gacgccgaag ctgccttcgc cgccgccagc gacattgacg acaacaccac
     4201 ggtggtcgag gctggtgtca acttcacatc cgccgcctat ggcctcgagg tggcatgggt
     4261 tcgcctcttc gccgatgccg gctgcatcga agacgaggcc caggcctcac aatgggtctc
     4321 cgactacgtc acagctctcc aaaccgacct ccaggcagca ggcctgtacg agggcgacat
     4381 cgacgggatc tacgggccgc agacgatcgc cgctgtagaa caactgcaga aggatgccgg
     4441 gcttcccgtg actggactcg tcgacccggc aacgcaggca gcgctggctg cggctctggg
     4501 gcagcgtcag tcggccgaga ttggtgccct tcagggaatc ctgatcagca ccggctatta
     4561 cgacggagtc gttgacggga tctggtcccg agaggtcgag gacgcactca aggcgctgca
     4621 aaccgatctc ggagtcccgg ccaccggcgt ggtcg
//
LOCUS       DBNHXU010000018         8499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_203481, whole genome shotgun sequence.
ACCESSION   DBNHXU010000018 DBNHXU010000000
VERSION     DBNHXU010000018.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8499
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_203481"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..506)
                     /locus_tag="AC161X_00530"
     CDS             complement(<1..506)
                     /locus_tag="AC161X_00530"
                     /inference="COORDINATES: protein motif:HMM:NF012531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit alpha"
                     /protein_id="HIR4988063.1"
                     /translation="MTAPFFDLKHFHDACGVGFIAALDRVPSYRMTRLAVECLERLDH
                     RGARAADGTGDGAGLLTQIPVRLISRELSAHGLEVAPERLGLVMCFLSPTHAAASRSV
                     VTQAIESEGMRVLFWRSVPFDPAMLGDHARDVLPLIEQAIVTGSEEGAAFERSLYLAR
                     KRIERAATP"
     gene            complement(603..1760)
                     /locus_tag="AC161X_00535"
     CDS             complement(603..1760)
                     /locus_tag="AC161X_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009309336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR4988064.1"
                     /translation="MVSDRLAPFGVTIFSEMTALAAEHGAINLGQGFPNWDGDQFVKE
                     AAARSMAEGGHDQYPPSPGIPELREAIATRYGPLLERELDPRSEITVTCGCTEALTAA
                     FLGLINSGDEVVLIEPFYDSYPVHISITGAVPRYVTLRPPDFHLDLGELEAAFSPKTR
                     AIVLNNPHNPTGHVFGMEELSAIAALCQEHDAFAISDEVYEEMVYGVPHLRLAGLPGM
                     WERTLTLSSLGKTFSLTGWKVGWAIGPSHLTAGVRAAHQFMTFTTPTPVQHGAVAALG
                     APQSLFDDLRSSYLAKRDLLGAGLVEAGFEVYQPQGTYFMMAGHARFGFSDDRAFCYH
                     LAEIARVVAIPPGVFYHRPEDGASLVRFAFCKDEKTLEEAIDRLKSFASKR"
     gene            complement(1828..2535)
                     /locus_tag="AC161X_00540"
     CDS             complement(1828..2535)
                     /locus_tag="AC161X_00540"
                     /inference="COORDINATES: protein motif:HMM:NF013856.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HIR4988065.1"
                     /translation="MTTETIVWKGRCGPFDLQVAPDTFRPSTISTLLADSMVIEPGSV
                     VIDAGCGSGVLSIVAAKLGAKMVYGVDAAAETVEIATANAKAQGVSDRTTFYEGDMFG
                     PIPDGVEADVVIGDVSGIPDEFAKVSGWFPSGLSGGPSGAELPMRMLDEAKRILRRGG
                     KLLLPTGTLQDENSILDKARSLFGSIRQLSERSIPLPSSLAEQPAVLGLIREKVIQFT
                     ERGSRLLWTVRVWEVSG"
     gene            complement(2582..3961)
                     /locus_tag="AC161X_00545"
     CDS             complement(2582..3961)
                     /locus_tag="AC161X_00545"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020513057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HIR4988066.1"
                     /translation="MFAASEFPAGFLWGVATAAYQVEGAVDEDGRAPSIWDTFSHTPG
                     RTHNGDNGDLAVDHYHRYADDVALMTELGVNAYRFSISWSRILPEGTGRVNEAGIDFY
                     RRVCKELLEVGIAPVVTLYHWDLPQALQDRGGWLNHESVDWFAEYATATKERLGDLIK
                     VWATFNEPWCSAFLGHSSGEHAPGIKEPGQSFVAAHHLMLAHHAGVKAMRSANRHDDD
                     QLGIVLNLIPAWPVTDSPEDEFAAASVDAVSNGLFTGAVFDGRYPAEILGYIDRYGVT
                     GQIDIDELSNAVQPIDFLGVNYYNINHVEHAPGEPAPGAWPGSWDARIATPPGVLTEM
                     GWGVEPHGLLWMLERVGREHPGIPLYICENGAAYPDRVSADGSVLDPERVAYLEGHIE
                     AVHTALQRGVDVRGYFVWSLLDNFEWGKGYDKRFGIVRVDLDTMERTIKASGHWYRDF
                     ISGRVGVRE"
     gene            complement(3998..5089)
                     /locus_tag="AC161X_00550"
     CDS             complement(3998..5089)
                     /locus_tag="AC161X_00550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014432608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR4988067.1"
                     /translation="MTVTLNPPPVDVISAPPPRKRKPDRQPLRKGWLSTSITALILFA
                     FLLPLAYMAVTSLKSEEMIANIGGPILPKSPATFEYEGKDLDMYTVPLPDGTEMGLAM
                     LKPGRQVSQFIDPANPETPIEWEGNWRILEPAYELDPQWVNYERAWELMNFPNLLRNT
                     LIIAGLGTIGAVISSILVAYGLARFRIPFGRSILVVLIATILLPSYVTLVPKYAFFVS
                     IGWVGTYLPLIVPHFFANAFNVFLLRQYFLTIPIELDEAATIDGAGAFRTLWSVVLPQ
                     MKAAIVAVCLFHFYFAWNDFFEPMIYLSTKPDLVPISVGLRTFNTIYDTQPTLIQAGS
                     LIALVVPVVIFLLAQRVFLQGIDLSGVQK"
     gene            complement(5091..5945)
                     /locus_tag="AC161X_00555"
     CDS             complement(5091..5945)
                     /locus_tag="AC161X_00555"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR4988068.1"
                     /translation="MPWIVGFFLWYAVPIVTSLWFSFTDFQLVTTEPTRFVGLANWQR
                     MFNDPEVRQSVLVTLKFGLIALPLTMIVPVTLAYLLVTRSLRGREGFRALFFMPSIIP
                     FVAAVLIFGGMFNPKTGWVNRILAVFGITGPDWFYDTTWVYPSLAFIGLWGVGNAMLL
                     FIASMNSVSASLYDAARIDGATELRTFWHITMPIISPIIFYNLILGLIGVFNYFLVPY
                     VLNNGTGAPNGATNFYALQFFKQGFKYFDMSYAATLAWALFLAALAVTGLVFWSAKYW
                     VYYEARRD"
     gene            complement(6078..7538)
                     /locus_tag="AC161X_00560"
     CDS             complement(6078..7538)
                     /locus_tag="AC161X_00560"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4988069.1"
                     /translation="MKQAKSTALVVVLLLILAACGESSGGSTTTAAAGEGSTTTAAAT
                     DTTAAPAGEPVTIRWFIGLGTGANPEQKEAQEAAVADFNASHPNIVLEIEIVDHDAAP
                     AALATEISSGNAPDIIGPVGREGANGFAGQYLDLEPLVESSGFDMSIWPEATVENMRD
                     QDGTLAGLPFASYPSEMYFSKALFDEAGLPYPPQEYGADGTAVYGPGTEWEGTWDFDK
                     VAEIAKILTVDENGNDATSPDFDIATRTQYGFVWQWTDRLFQQGSFWGAGYPMADDGS
                     AAFPQEWVDEWHWYYDAIWTSGFAPGQADYDAMGGNAFQSGKAAMGISHLWFTCCIRN
                     DDSGVAGDFYDLAVLPSHNGKVTANMHADTFRVLSSTEHPEEAFEVIAYLVTDGALPL
                     LTTYGAAPADPTKVADFLATLDERYPQGVNWQVALDGANFADTPPHESFVPGWQQYVD
                     RMGVTLTAMLTDGSLDLDAEIATLESDLTTIFQTNQ"
     gene            complement(7635..>8499)
                     /locus_tag="AC161X_00565"
     CDS             complement(7635..>8499)
                     /locus_tag="AC161X_00565"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR4988070.1"
                     /translation="LAARNLASGKSGVVGVVMHVAAQLTFSDNYFAGLLTGICDSLTE
                     SAAGMMLWLGNRTKEETLDQILSMGMLDGIIVTADTIDDPLVDGLRSSGVPSILIGHR
                     RADNDASYIDIDNEAAAEAIADHLIGLGRKRIAHITGRRDSVSSRDRKTGYRRALRRA
                     NLPTEGLIIEGDYTAEGGYKAATSLIEAKVDAIFCASDNTAAGALDAIRNAGLRVPDD
                     VALAGFDDLAFASELAPPLTTMRQDIQGIGQEAARNLLTLLDRPEGGPRRVLLPTELV
                     IRQSTVGGLSG"
BASE COUNT         1745 a         2564 c         2640 g         1550 t
ORIGIN      
        1 ggggtggcgg cccgttcgat tctcttccga gccaggtaga ggctccgctc gaaggctgcc
       61 ccctcctcgg agccggtcac gatcgcctgc tcaatcaggg gaaggacgtc acgggcgtga
      121 tcgccgagca tcgctggatc gaacggcacc gatctccaaa acaggacccg catcccttcg
      181 gactcgatcg cctgggtcac caccgatcgg gaggcggcgg catgggtggg agacaagaag
      241 cacatcacaa gtccgagacg ctcgggggcg acctcgagcc catgggcact cagttcccga
      301 ctgatgaggc gaaccggaat ttgagtaagg aggcctgcac catctccggt tccatccgcc
      361 gcccgcgcac ctcgatgatc gagtcgttcc aggcactcca cggcgaggcg ggtcatccga
      421 tacgagggga cgcgatccag agcagcgatg aacccgacgc cgcaggcgtc gtgaaagtgc
      481 ttcagatcga agaaaggtgc cgtcattgct ccctcgaaat gggggagcga tcaagtttaa
      541 acacgtccca ggtcgcaagt cccaggtccc aagcttctgg cttaggactt gagacctgag
      601 actcaacgct tcgaagcgaa agacttcagc cggtcgattg cttcttctag cgtcttttca
      661 tccttacaaa aggcgaatct caccaacgag gccccatcct cggggcggtg atagaaaact
      721 cccggcggaa tggccaccac ccgggcgatt tccgcaaggt ggtagcaaaa ggcccgatcg
      781 tcggaaaacc cgaaacgggc atgcccggcc atcatgaaat aggtgccctg gggctgatag
      841 acctcgaacc cggcctcgac caagcccgct cccagcagat ctcgcttggc gagataactg
      901 gagcggagat catcgaacag agactggggt gcgccgagcg cagccacggc tccatgctgg
      961 acgggggtcg gagtggtgaa ggtcatgaat tgatgggcag cacgcacccc cgccgtcagg
     1021 tgggaaggcc caatcgccca tcccaccttc catccggtga gcgagaaggt ctttcccagt
     1081 gatgaaaggg tcagggttcg ctcccacatg ccgggaagcc cggcaagtct gaggtgaggc
     1141 actccgtaga ccatctcctc gtacacctcg tctgagatcg cgaaagcgtc gtgctcctgg
     1201 cacagggcgg cgatggcgct cagctcctcc atgccaaaga catgaccggt tgggttgtgg
     1261 ggattgttga gaacgatggc gcgggtcttc ggtgagaagg cggcctcgag ctcacccagg
     1321 tcgaggtgga agtccggtgg tcgcagtgtc acgtacctgg ggacagcgcc tgtgatcgag
     1381 atgtgcaccg gataggagtc atagaagggc tcgatcagga ccacctcgtc ccccgagttg
     1441 ataaggccta ggaatgcggc ggtcagggcc tcggtgcacc cgcaggtgac agttatctca
     1501 ctgcggggat cgagttcccg ctcgagaaga ggtccatatc tcgtggcgat cgcttcacgc
     1561 aactccggaa ttccggggct gggcggatac tggtcatgcc ctccctcggc catcgacctc
     1621 gccgccgcct ccttgacgaa ctggtcgccg tcccagttgg gaaacccctg accgagattg
     1681 atcgcaccgt gttcggcggc aagggcggtc atctcgctga agatggtcac cccgaacgga
     1741 gcgaggcggt cagacaccat gggtctcagg ttacgaggcg cagagtttct ggcttgagat
     1801 ctgagacttg ggacctgaga ccggctttca gccggaaacc tcccacaccc gcaccgtcca
     1861 gagcaggcga cttccccgct cggtgaactg gatgaccttt tcgcgaatca ggccaaggac
     1921 cgccggctgc tctgcgagcg acgagggaag agggatgctg cgctcggaga gttggcgaat
     1981 cgacccaaac aaagacctcg ccttgtcgag aatcgagttc tcatcctgaa gagtgcctgt
     2041 cggcaacaag agcttgccgc cacgacgaag aatgcgcttg gcctcgtcga gcatcctcat
     2101 tgggagctcg gcgccgcttg gtcccccgga gagccctgag ggaaaccagc cggacacctt
     2161 tgcgaactca tcagggatgc cggaaacgtc gcctatgaca acatcggcct caacgccatc
     2221 agggatgggg ccaaacatgt caccctcgta gaacgtcgtg cgatcactta ccccttgggc
     2281 tttggcatta gcggtggcga tttcaactgt ctcggccgcg gcgtccactc cgtagaccat
     2341 cttcgccccg agcttggcgg cgacgatcga gagaaccccg gagccgcacc cggcgtcgat
     2401 caccacggaa cccggctcga tgaccatcga gtcggcaagc agcgtcgaaa tcgtcgacgg
     2461 gcggaaggtg tccggagcca cctgcagatc gaatgggccg caacggccct tccagacgat
     2521 ggtctcggta gtcatgtcaa tcagacacta tccaaaggga ttcggttact gcacggcggg
     2581 ttcattctcg gacgccgacg cggcctgaga tgaagtcccg ataccaatgc ccactggcct
     2641 ttatggtccg ttccatggtg tccaagtcga cccgaacgat gccaaaccgc ttgtcgtaac
     2701 cctttcccca ctcgaaattg tcgagcaggg accagacgaa gtagccacgg acgtcgacac
     2761 ctcgttgcag tgcggtgtga accgcctcga tatggccctc caggtaggcg acgcgctcgg
     2821 gatcgagcac agatccgtcg gccgacacac ggtcggggta ggcagcaccg ttctcacaga
     2881 tatagagcgg gatcccgggg tgctcacgac ctactcgctc cagcatccag agcaggccgt
     2941 ggggctcgac accccaaccc atttcggtga gaacgcctgg tggcgtggcg atcctcgcgt
     3001 cccacgaacc cggccaggca cccggggcag gctcgccagg agcatgctcg acatggttga
     3061 tgttgtagta gttgactccg aggaagtcga tcggctgcac agcgttcgac agttcatcga
     3121 tgtcgatttg gcccgtgacc ccgtaacgat cgatatatcc gagtatctcg gccggataac
     3181 ggccgtcgaa gaccgcgccg gtgaacagcc cattgctgac cgcatctacc gaggctgcag
     3241 caaattcatc ctcgggcgaa tcagtgactg gccaggccgg tatgagattg agcacgatcc
     3301 ccaactgatc gtcgtcgtgg cggttggcgc ttctcatcgc cttcaccccg gcgtggtggg
     3361 caagcatcag gtgatgggcc gccacgaatg actggccggg ctccttgatg ccaggggcgt
     3421 gctcgccaga gctgtgaccg agaaaggccg agcaccacgg ctcgttgaac gtcgcccaga
     3481 ccttgatcag atcccccagg cgctccttgg tggcggttgc gtattccgcg aaccagtcaa
     3541 cggactcgtg gttgagccag cctccccgat cctggagtgc ctgtgggaga tcccagtgat
     3601 acaaggtgac cacgggagcg atcccgactt ccagcaactc cttacacacc cttcggtaga
     3661 agtcgatccc cgcttcgttg actcgacctg tcccctcggg aaggatccgg gaccatgaaa
     3721 tcgagaatcg gtatgcgttg actcccaact ccgtcatgag agccacgtcg tcggcgtagc
     3781 ggtggtagtg gtcaacggcg agatctccgt tgtcgccgtt gtgcgtccgg cccggcgtgt
     3841 ggctgaacgt gtcccagatc gatggggcgc gaccatcttc gtctaccgcg ccctcgacct
     3901 gataagccgc ggtggcgacg ccccatagga aaccggccgg gaactcagat gcggcgaaca
     3961 tttgcgcccc tagccatcct cattgctcga cccccgacta cttctgcaca ccggacaggt
     4021 cgatcccctg aaggaacacc ctttgggcta ataggaagat cacgactggg acaacgagag
     4081 cgatcagcga acccgcctga atcaaggtgg gctgagtgtc gtagatcgtg ttgaacgtgc
     4141 gcaaacccac cgagatcggc accagatcag gcttggtcga aagatagatc atcggctcga
     4201 agaaatcgtt ccaggcgaag tagaagtgga acaagcaaac ggcaacgatc gctgccttca
     4261 tctggggcag aaccaccgac cacagggttc tgaatgcccc agcgccgtcg atggtggcag
     4321 cctcgtcgag ttcaatgggg atcgtcagga agtactggcg caacaggaag acgttgaatg
     4381 cattggcaaa gaaatggggg acgatcaagg gcaagtaggt gccgacccac ccaatggaga
     4441 caaagaaggc gtacttgggc accagggtga catagctggg aagcaggatc gtggcgataa
     4501 gcacgacgag aatcgatctg ccgaacggga tcctgaatcg ggccaggccg taggcgacga
     4561 ggatcgagga gatgactgca ccgatggtcc cgagaccggc aatgattagc gtgttgcgca
     4621 gcaggttggg gaaattcatg agttcccacg cccgctcgta gttcacccac tgcgggtcca
     4681 gctcgtacgc cggctccaag atccgccagt tgccttccca ttcgattggg gtctccgggt
     4741 tagccgggtc gatgaattgg gacacctgcc ggcccggctt caacatcgcc aggcccatct
     4801 cggttccgtc cgggagcggg accgtgtaca tgtccaagtc cttcccctcg tactcgaacg
     4861 tagccggcga cttgggaagg atcggccccc cgatattggc gatcatctcc tcggacttca
     4921 gcgacgtcac ggccatgtag gccaggggaa gcaggaaagc aaagagaatc aatgccgtga
     4981 tcgaggtcga tagccagccc ttgcgcagcg gttgacggtc cggcttgcgc ttgcgagggg
     5041 gtggagccga aatcacatcg acgggaggcg ggttcagagt gacggtcacc tcagtccctc
     5101 ctcgcctcgt aatacaccca atacttcgcc gaccagaaaa ccagccccgt caccgcgagg
     5161 gcggcgagga aaagcgccca ggccagggtg gcggcataac tcatgtcgaa gtacttgaag
     5221 ccctgtttga agaactggag ggcatagaaa ttggtcgcgc cgttgggagc gcccgtgccg
     5281 ttgttgagca cataaggaac caggaagtag ttgaacaccc cgatcaggcc gaggatcagg
     5341 ttgtagaaga tgatcgggga gatgatcggc atcgttatat gccagaaagt tcgcaactcg
     5401 gttgcgccgt cgatcctcgc cgcgtcgtac aacgatgcag aaacggagtt catcgaggca
     5461 atgaagagca gcatggcatt gccgacaccc cacaagccga tgaaagcaag tgaggggtac
     5521 acccaagtcg tgtcgtagaa ccagtcggga ccggtgatcc caaagacggc aaggatccga
     5581 ttcacccatc ccgtcttggg attgaacatt cccccaaaga tgagaaccgc cgcaacaaag
     5641 gggatgatcg acggcatgaa aaagagggct cggaagcctt ccctgccccg caagctcctg
     5701 gtgaccagaa gataggcgag ggtcacggga acgatcatcg taagaggaag agcgatgagc
     5761 ccgaacttga gggtcaccaa gactgactgc ctcacttcgg ggtcgttgaa catcctttgc
     5821 cagttcgcca ggccaacgaa acgcgttggc tcagtggtga ccagttggaa gtcggtgaac
     5881 gagaaccaga gtgaggtgac gatcggcacc gcgtaccaga gaaagaaccc cacgatccaa
     5941 ggcatcacga accagagtcc gacgcggccc cgccgtttgg ctagtgagac tgtcttcttg
     6001 gtcttgggtg ttgatggggc ggccggaaga ccggccgccc catcttcggt aatagctgtc
     6061 aaatcaccaa caactaacta ctggtttgtt tggaagatag tcgtcaggtc tgactccaac
     6121 gttgcgatct cggcgtcgag atcgagcgaa ccgtcggtga gcatggcggt caaggtgacc
     6181 cccatccggt cgacgtactg ctgccatccg ggaacgaagc tctcgtgtgg cggagtgtcc
     6241 gcaaagttgg caccgtccaa tgccacctgc cagttcacac cctgggggta cctctcatcg
     6301 agagtcgcca ggaagtcggc caccttggtc gggtcggccg gggccgcacc gtaggtggtc
     6361 aacaacggga gagccccgtc ggtaaccagg taggcgatga cctcgaaggc ctcctcaggg
     6421 tgctccgtcg acgacaggac gcggaaggtg tcggcgtgca tgttcgctgt caccttgccg
     6481 ttgtgtgacg gcagtacggc gaggtcgtag aagtcgccgg cgacaccgct gtcatcgttg
     6541 cggatgcagc aggtgaacca gagatggctg atgcccattg cagccttacc agactggaag
     6601 gcgttgccgc ccattgcgtc gtagtcggcc tgacctggcg caaaccccga cgtccaaatg
     6661 gcgtcgtagt accagtgcca ctcatccacc cactcctgcg ggaaggctgc cgagccgtca
     6721 tcggccatcg gataaccggc tccccagaag gatccctgct ggaacagacg gtcagtccac
     6781 tgccagacga acccgtactg ggtccgcgtg gcgatgtcga aatccgggct ggtggcatcg
     6841 ttgccgtttt cgtcgacagt caggatcttg gcgatctcag cgaccttgtc gaagtcccag
     6901 gtcccttccc actccgttcc ggggccatag acggcggtgc cgtcggcgcc atattcctgg
     6961 ggcggatagg gcagaccggc ctcgtcgaac agggccttgc tgaagtacat ctccgaggga
     7021 tacgaggcga acggaagacc ggcaagggtc ccgtcctggt cccgcatgtt ctcgacggtg
     7081 gcttcgggcc aaatcgacat gtcgaacccg ctggactcga ccaacggctc caggtcgaga
     7141 tactggccgg caaacccgtt ggcaccttca cgacccacgg ggccgatgat gtcgggggca
     7201 ttgcctgacg agatctcggt ggccagagct gcaggggcgg cgtcgtgatc cacgatctcg
     7261 atctccaaca cgatgttggg gtgcgaggcg ttgaaatcgg cgacagctgc ttcctgagct
     7321 tccttctgtt cggggttggc accggtgccc aacccgatga accatcgaat tgttacgggc
     7381 tcgcctgccg gcgctgcggt cgtatccgtc gcagctgccg tcgtcgtgga cccttctcca
     7441 gccgccgccg tcgtggtgga accaccactg ctttcaccac aagcggcgag gatcaagagg
     7501 aggaccacca cgagggcggt actctttgct tgcttcatct cttccgttct cctccgtcgc
     7561 ccccggacat gtcaccgatc gttacgatcg atgcggccgc actccgggga cgggcgcgac
     7621 cgtgctactt cgggctatcc cgatagacct ccaaccgtcg attgccttat caccaactcg
     7681 gttggaagaa gtactcgccg tggcccgccc tcggggcggt cgaggagcgt gaggaggttg
     7741 cgtgcggcct cctggccaat cccctggatg tcctggcgca tcgttgtcag cggcggggcg
     7801 agctcgctgg cgaatgcaag gtcgtcgaac ccggccagcg ctacgtcgtc ggggacgcgc
     7861 aatccggcgt tgcgaatcgc atccagcgcc ccggccgccg tgttgtcact cgcacagaag
     7921 atggcatcga ccttggcctc gatgagactg gtggccgcct tgtatccacc ttcggccgtg
     7981 tagtcgcctt cgatgatcag gccttcggtg gggagattgg cccggcgcag cgctcgtcgg
     8041 taaccggtct tgcgatcccg actcgagaca ctgtcacgtc ggccggtgat gtgggcgatt
     8101 cgtttccgtc ccagtccgat caaatggtcg gcgatggcct cggcagccgc ttcgttgtcg
     8161 atgtcgatgt agctggcatc gttgtccgcg cgtcggtggc cgatgaggat gctgggcaca
     8221 cccgaggagc gaaggccgtc gaccaagggg tcatcgatgg tgtcggcggt gacaatgatc
     8281 ccgtcgagca tccccatgga gaggatctgg tcgagagtct cttccttggt ccgattgcca
     8341 agccaaagca tcatccccgc cgccgactct gtcagcgagt cgcagatacc cgtcaacagg
     8401 ccggcgaagt agttgtccga aaaggtcagc tgtgcggcga catgcatcac gacgccgacg
     8461 acgccggact tgccgctggc gaggttgcgt gcggcgagg
//
LOCUS       DBNHXU010000019        10676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2584544, whole genome shotgun sequence.
ACCESSION   DBNHXU010000019 DBNHXU010000000
VERSION     DBNHXU010000019.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 10676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10676
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2584544"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3478)
                     /gene="dnaE"
                     /locus_tag="AC161X_00570"
     CDS             complement(<1..3478)
                     /gene="dnaE"
                     /locus_tag="AC161X_00570"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013131676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HIR4988071.1"
                     /translation="MTAVSFAHLHVHTEYSMLDGAARIRDVVSAAAADGQPAIAITDH
                     GVLYGVVDFVKTAREIGIKPIIGIEAYFTDGSRFDRPLGGANKRFHMNLLAVSEVGYR
                     NLLQLSSKAFLEGYYYKPRMDFELLAQHAEGIIATSGCLGGMVPQLLAPDSMSEEGNK
                     GQIRDYEAALAAAGKFQDIFGKENFFIEVQDHGLPAQKAIMPDLLAISTHTGAPLLAT
                     NDAHYTRRNEHDAHDVLLCIQTGSLRSDTSRLRFEGSDHYLKTAAEMRQLFPDDTFPG
                     ACDNTLLVAERAEVELELGRILLPHFPVPTGETEVTYLRKLVDQGTRERYGHNPAPEV
                     WDRVEHELKIIEEMGFPAYFLIVWDLIRYARENRIRVGPGRGSAAGSIVSYCLRITDI
                     DPLAYGLIFERFLNPGRKEMPDIDMDFDERYRSDVIRYASQKYGADRVAQIVTFSTIK
                     GKQAVRDAARVLGHAYGVGDRIAKAMPPPILGKEATLEQVLVAPPADADSSVKDWYSN
                     AQGLRELYESDEAVRETVDAARGVEGLRRQDSIHAAAVVIGPEPLINIVPIQQKGEGA
                     EIVTQYEMYGVQSLGLLKMDFLGLRNLSIIERCLELVEETTGERVDIDSVPLDDEKTF
                     DLLQSGNTIGVFQLEGTAMRSLIRSLRPDSFNDVIALVALYRPGPMGANMHNLYADRK
                     NGRAEIEQLHPALTDKLEDTYQIMVYQEQVMTVAQEMAGYSMVEADDLRRAMGKKIKK
                     EMLAQEERFIQGCMSQGHTEETGKEIFGLIEHFAGYGFNRSHSAAYGLVAYQTAYLKA
                     HHPAEYLAALLTATKKDKDRTAVYLNECRQMGIKVLVPDVNESDSDFTVREGRIRFGL
                     SAVRNVGDGVVEKIVEARAEEPFTSLQDFVDRVDVSALNKRTLESLIKAGAFDASGDP
                     RKGLTMVYEQILDATLERRRNEDMGQFSLFAGDTEAVQESHIEVPALVWPQKIRLGFE
                     KEMLGLYVSDHPLLSIGPSLAAATSNGIHDLGELADRSSVVVGGLVASITRRWTRAGE
                     PMIFFQLEDLEGSIEVMAFPRTVHDFGPAIIEDAIVVVSGNLDNRGDEVKVIAREIKE
                     LEVKDDNSVRLQVAAGRLSPEVVTRLKGILSNHPGSAVVYLHMVGDNGTKVLKLSDEH
                     RVEPRSALFAELK"
     gene            complement(3490..4323)
                     /locus_tag="AC161X_00575"
     CDS             complement(3490..4323)
                     /locus_tag="AC161X_00575"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: protein motif:HMM:NF013827.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR4988072.1"
                     /translation="MTQENTTEAPVCYRHPDRQTLLACSRCGRPICAACSIDASVGQR
                     CPECVREEGGQKVIPTGPRRRTSAATGAPATMVFIALSVVFYLASNFGAFGDQIFSAL
                     AQDNQAVAAGEWWRIFTVVLLHGSITHILFNMWALWVLGPQVERGVGTWPFVSLYIAA
                     AGMGGVAAYYFGGPFDVGVGASGAIFGLFGLWISWAVHRRNTAYGRAVLNQLGFLLLI
                     NAAMPFIFPGISWQAHLGGLITGYLIGEVWAKIQGPNATAIRAAVGFAVAALAAVAVS
                     L"
     gene            complement(4352..5272)
                     /locus_tag="AC161X_00580"
     CDS             complement(4352..5272)
                     /locus_tag="AC161X_00580"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HIR4988073.1"
                     /translation="MADTLTFEVPEPLDGMRVDKAIASLLGVSRFVARELVDRGVVLD
                     GNAARPRDRVRTGSVIETPHPAVELSLQPEDVPFDVLYEDDALLVVDKPAGVVVHPGS
                     GTKEGTLAAGLLNRYPELEGVGEPGRWGLIHRLDKDTSGTLIVARTEDAYRSLVDDLS
                     ARRIARTYITLVYGLFPVPTGTIDAPIGRDPSRPTRRAVSPTGKSAVTHYELIEEFPQ
                     HEVALLEVNLETGRTHQIRVHLAAIEHPVIADRTYSPFNTRVKSPRIFLHARRVRLNH
                     PVSGAEVSVESPLPEDLQTVLDGLRSGSAS"
     gene            complement(5257..5712)
                     /gene="lspA"
                     /locus_tag="AC161X_00585"
     CDS             complement(5257..5712)
                     /gene="lspA"
                     /locus_tag="AC161X_00585"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HIR4988074.1"
                     /translation="MIRRYALAAGIAAFVVTIDALTKRWASIHFVGNPVEVIPGFLTF
                     TYTENPGAAFGSLQSAGPFLGLAAIGVTLGLLWAIRHPRPTLEIVAFGLIMGGALGNL
                     VDRIFRGPGLLDGKVIDWVNLWWIPTFNLADTSITIAVVLLLIQSWRTR"
     gene            complement(5709..6080)
                     /locus_tag="AC161X_00590"
     CDS             complement(5709..6080)
                     /locus_tag="AC161X_00590"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR4988075.1"
                     /translation="MARNLAKSTVERYRKRLEDERERLLVMIRDYQLELEEARLTESS
                     SDRSPDPGNAEAGSMKFEYEKELSIEQNTVDLLGKVERALENIENGRYGICESCGKPI
                     PMARLDVLPYVTLCVDCARKS"
     gene            6190..9369
                     /gene="ileS"
                     /locus_tag="AC161X_00595"
     CDS             6190..9369
                     /gene="ileS"
                     /locus_tag="AC161X_00595"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004822 - isoleucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006428 - isoleucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HIR4988076.1"
                     /translation="MTNETGFQRVDPAVDIPRLEEAVLALWRDTDAFAESVRRRPADK
                     EYVFYDGPPFPTGSPHYGNLLAGVIKDMVPRYWTMRGYRVERRFGWDTHGLPIEMEVE
                     KQLGISGPRQIAEYGIDRYNEACRIGVQANTEAWETLTERIGRWVDFENDYKTMDPDF
                     METVWWVFKQLWDKGLIYRDFKVLPYSWGATTPLSNFEANLDYRDVEDPSITVRLAVV
                     EGNDKAEAGDYLLIWTTTPWTLPGNLAVAIGPGNEYLRIADGGDHYWIAAERVESVFG
                     NRGVTVARVKGAELLGVGYEPPFDHFADRWHVGAFRTIPSDEVSVDEGTGLVHMAPAY
                     GEADFLALQAAGIEALVDPVDAGGNFTDDVPEVAGRNVKEADAILIDLLRGSGKLVHS
                     GRIVHSYPYCYRTGTPLIYKAIPTWFVAVEQFRDRMVELNETVHWVPEYVGSRRFGNW
                     LANARDWAISRNRYWGSCIPVWQCDSCEDRVAIGSVDELEGLSGQRPTDLHRHFIDLI
                     TWPCASCDGVMTRVPEVLDCWFESGSMPYGMNHYPFENTERFEATFPAKFIAEGLDQT
                     RGWFYTLIVLSTALFDRPAFENCVVNGLILAEDGRKMSKSLKNYPDPSEIFDQHGADA
                     VRAFLINSPVLRAEPLRFSKDGVADVVRTVLLPLWNTYSFFTTYAEADGLTTSDFADA
                     PPVEERPEMDRWILSVLQSLVAEVNTQMEGYYLYSVVPPILGFIDHLTNWYVRRSRRR
                     FWRARGESGEGDVDKLAAFATLYQVLTTFIEVLAPILPFITEHLYQDLIARHDPDAVR
                     SVHHRDFPEADETLIDSELESSMAVVREVVRLGRNLRKKEGHRVRQPLAGLTILTHDP
                     DIGAAVETHGDIISEELNVKKVTATDDEGALVHLSAKANFRKLGPTLGSEMQTVAAGI
                     AALGGDDLAGILDGGSVEVAGHSINLDDLIVERTPVEGVIVETGADFACALDTTLDDD
                     LVLEGVAREIVSRVQKLRREAGLDVVDRISLTWHSLDPQVAAAFDTHHDVIAGEVLAS
                     SVDSSSAEVGDSFDLDGRTVWLSLVRTDS"
     gene            complement(9373..10278)
                     /locus_tag="AC161X_00600"
     CDS             complement(9373..10278)
                     /locus_tag="AC161X_00600"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009987 - cellular process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR4988077.1"
                     /translation="MPITPSDIEHKTFSTALRGYDLDEVDDFLDEIVVALRDLHGELT
                     AAKAKIAELEQSKGPEVPAVAAVATSVAVGDEASVGRVLILAQQAADRTVDEARTEAD
                     RLLEEARGEADRTLTEARTEADSFEAEREERRTAAQTEMAELTVRVSSVRNQLALLAT
                     SVADKLDEMDSTIESADLAAEDDDVDDMADLEDMTSETVDDGEDLELALDDEMDDAES
                     DEELTTDLTTVDDEAEAEDPDAMIDESEELDLDAASEEAEIEEDAVEDSVEDVEEDGV
                     EEVIEEAGDEVTDEVDGVEKAENSD"
     gene            complement(10336..10605)
                     /locus_tag="AC161X_00605"
     CDS             complement(10336..10605)
                     /locus_tag="AC161X_00605"
                     /inference="COORDINATES: protein motif:HMM:NF014389.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HIR4988078.1"
                     /translation="MDPIGIIILILQVYFYAVLAWVILSWIPTSSTHPLGKVSIFLDR
                     IIYPVILPLRRVIPPLRLGGGMLDLTPIVLLIGINILIGILRRVG"
BASE COUNT         1969 a         3387 c         3312 g         2008 t
ORIGIN      
        1 ccttcaactc ggcaaagagg gccgacctcg gttcgacccg gtgctcatcg ctcagtttca
       61 agaccttggt tccgttgtcg ccgaccatgt gcagatagac gacggctgag cccgggtggt
      121 tggagaggat ccccttgagg cgggtgacca cttcggggga aaggcgccct gccgccacct
      181 ggaggcggac cgagttgtcg tccttgacct ccagctcctt gatctccctg gcgatcacct
      241 tgacctcatc gccacgattg tcgaggttgc cactgaccac cacgattgcg tcttcgatga
      301 tcgccgggcc gaagtcgtga acggttcggg ggaatgccat gacttcgatc gatccctcga
      361 ggtcctccag ttggaagaag atcatcggct cccctgcccg cgtccaacgg cgggtgatgg
      421 aggcgaccaa gccgccgacc accaccgagc ttcgatccgc cagctcgccg aggtcgtgaa
      481 tcccgttgct ggttgcagcg gccaacgagg ggccgatcga gagcaaagga tgatccgaga
      541 catagagccc caacatctcc ttctcgaagc cgaggcggat cttctggggc cagaccagtg
      601 cgggcacctc gatgtggctc tcctggactg cttcggtatc gccggcaaac aggctgaact
      661 ggcccatgtc ctcgttgcgc cgacgctcga gcgtggcatc gagaatctgc tcatagacca
      721 tggtcaggcc tttgcgcgga tcacccgagg cgtcgaaggc gccggccttg atcaacgatt
      781 cgagggtccg cttgttgagt gccgacacgt cgaccctgtc gacgaaatcc tggagtgagg
      841 tgaagggctc ctcggccctg gcctcgacga tcttctcgac cacaccgtcg cccacatttc
      901 gcaccgcaga gagcccgaat cggatcctcc cctctcgcac tgtgaagtcg ctgtccgact
      961 cgttgacgtc aggaaccagc accttgatcc ccatctgccg gcactcgttg agatagaccg
     1021 ccgttcggtc cttgtccttc ttggtggcgg taagcagagc ggccaggtac tcggccgggt
     1081 ggtgggcctt gaggtacgcg gtctgatagg cgaccaggcc ataggcggcg ctatgtgatc
     1141 tgttgaagcc atacccggcg aagtgctcga tgagcccgaa gatctccttg ccggtctcct
     1201 cggtgtggcc ctggctcatg cagccctgga tgaatcgctc ctcctgggcg agcatctcct
     1261 tcttgatctt cttgcccatt gccctacgca ggtcgtcggc ttcgaccatc gagtagccgg
     1321 ccatctcctg ggcaaccgtc atcacctgct cctggtacac catgatctga taggtgtctt
     1381 ccagcttgtc ggtcagggcg ggatgcaatt gctcgatctc ggcccggccg ttcttgcggt
     1441 cggcgtagag gttgtgcatg ttcgccccca tcggaccggg gcggtaaagg gcgaccaaag
     1501 caatcacgtc gttgaatgag tcgggtctca gcgacctgat caaggaccgc atcgccgtcc
     1561 cctccaactg gaacacaccg atggtgttcc cgctctggag gaggtcgaaa gtcttctcgt
     1621 cgtcgagagg caccgagtcg atatcgaccc gctctccggt ggtctcctct acgagctcga
     1681 ggcaacgctc gatgattgag aggttgcgta ggccgaggaa gtccatcttg agcaacccga
     1741 gggactgcac accgtacatc tcgtactggg tgacgatctc ggcgccctcg cccttctgct
     1801 ggatggggac gatgttgatg agcggctcgg ggccgatgac gacggccgcg gcatggatcg
     1861 agtcctggcg acggagcccc tccaccccac gagccgcatc caccgtctca cgaaccgcct
     1921 cgtcactctc gtagagctcg cgcaacccct gggcgttgga ataccagtct ttgacgctcg
     1981 agtcggcgtc ggccggcggg gcgaccaaca cctgctcgag ggtggcctcc ttgcccagaa
     2041 tcggtggcgg cattgccttg gcgatccggt cgccaactcc gtaggcgtga ccgagcaccc
     2101 gggccgcatc acgcaccgcc tgcttgccct tgatcgtcga gaacgtgacg atctgggcca
     2161 cgcggtcggc gccgtacttc tgcgaggcgt atcggatgac gtcggagcga tagcgctcgt
     2221 cgaagtccat atcgatatca ggcatctcct tgcgccccgg gttgaggaag cgctcgaaga
     2281 tgaggccgta cgcaaggggg tcgatgtcgg tgatcctgag gcagtaggag acgatcgaac
     2341 cggcggcgga tcccctccca gggccaacgc gaatccggtt ctcccgggcg taccggatga
     2401 gatcccaaac gatgaggaag taggcgggga agcccatctc ctcaatgatc ttcagctcgt
     2461 gctcgacccg gtcccagacc tccggagccg ggttgtgccc gtaacgctcc ctggtgccct
     2521 ggtcgaccag ctttcgcaga taggtcactt cggtctcgcc ggtgggaacc gggaaatggg
     2581 gcagcaagat ccgccccagc tccaactcga cctcggcccg ctcggcgacc aacagcgtgt
     2641 tgtcacaggc cccggggaac gtgtcgtcgg ggaacagctg gcgcatctcg gccgcggtct
     2701 tcaggtagtg gtcacttccc tcgaaacgaa gccgggaggt gtcgctgcgc aacgagccgg
     2761 tctgaatgca gagcaggacg tcgtgagcgt cgtgctcatt gcgcctggtg tagtgggcgt
     2821 cgttggtcgc caacagaggg gcgccggtgt gagtcgagat ggcgagaagg tcgggcatga
     2881 tcgccttctg tgccgggagc ccatggtcct gcacctcgat gaagaagttc tccttgccaa
     2941 agatgtcctg gaacttgccg gctgcggcca acgccgcctc gtagtcacgg atctggcctt
     3001 tgttgccctc ctcgctcatc gagtccggcg ccagcagttg gggcaccatc ccgccgagac
     3061 agccggaggt ggcgatgatc ccctcggcat gctgcgccag cagttcgaag tccatccgag
     3121 gcttgtagta gtagccctcg aggaaggcct tggatgagag ttgaagcagg ttgcggtatc
     3181 cgacctcgct cacggcgagg aggttcatgt ggaagcgctt gttggccccg cccaggggcc
     3241 gatcgaatcg cgatccgtcg gtgaaatagg cctcgatccc gatgatcggc ttgatcccga
     3301 tctcccgcgc agtcttcacg aagtcgacga ccccgtagag gacgccgtgg tcggtgattg
     3361 cgatcgccgg ctgaccgtcg gcggccgccg cagagaccac gtcccgtatc cgggccgcgc
     3421 cgtcgagcat cgagtattcg gtgtgaacgt ggaggtgggc gaatgacacg gctgtcattc
     3481 aaccttacct cataacgaca cggcgacggc ggccagggcg gccaccgcaa agccgactgc
     3541 ggcgcgaatc gcggtcgcat tcggcccctg gatcttcgcc caaacctcgc cgatcaggta
     3601 tccggtgatc agccctccga gatgggcctg ccaggagatg ccagggaaga tgaaaggcat
     3661 tgccgcgttg atcagcagca gaaagccgag ttggttgagg accgcccggc cgtaggccgt
     3721 gttgcgccgg tgcactgccc aactgatcca gagcccgaac aacccgaaga tggcgcccga
     3781 tgcgccaaca ccgacatcga acgggccgcc gaagtagtaa gcagccactc cccccatccc
     3841 ggccgccgcg atgtagaggc ttacaaaggg ccaggtgcca acccctcgct ccacttgggg
     3901 gcccagtacc caaagcgccc acatgttgaa caggatgtgg gtgatcgacc cgtggagcag
     3961 gacgacggtg aagatcctcc accactcacc agcggccacc gcctgattgt cctgagcgag
     4021 agccgagaag atctggtccc cgaaagcgcc gaagttcgac gccaggtaga agacgacgga
     4081 gagggcgatg aacaccatgg tcgccggggc tcctgtcgcc gccgaagtcc ggcgcctggg
     4141 gccggtgggg atcaccttct gcccaccctc ctcacgcaca cattcgggac agcgctggcc
     4201 taccgaggca tcaatcgagc aggcagcaca gatcggacgg ccacagcggc tgcatgccaa
     4261 aagggtctgg cgatctggat ggcgataaca gaccggcgcc tcggtggtgt tctcttgagt
     4321 cacttgatca aacggtagat atctcggttt cttagctggc ggaacccgac cggagaccat
     4381 ccagcactgt ctgcagatct tcgggaagag gcgattccac actgacctcg gcgcccgaca
     4441 ccggatggtt gaggcggacc cgacgggcgt ggaggaagat tcggggggac ttcaccctgg
     4501 tgttgaaggg cgagtaggtc cgatcggcga tcaccggatg ttcgatcgcc gccaggtgga
     4561 cccggatctg atgtgtcctc ccggtctcga ggttgacctc gagcagggcc acttcgtgct
     4621 ggggaaactc ttcgatcaac tcatagtggg tgacagccga ctttcccgtt ggtgagaccg
     4681 cccttcgtgt ggggcgggaa gggtcgcgtc cgatcggggc atcgatggtc cctgtcggca
     4741 ccgggaacag gccatatacg agggtgatgt aggtcctggc gatcctcctc gcgctcaggt
     4801 catcaaccag ggagcggtag gcgtcctcgg tgcgggccac gatcaacgtc cccgaggtgt
     4861 ccttgtccag acggtggatc aggccccacc gccccggttc cccgacccct tccaactccg
     4921 gatatcggtt gaggaggccg gcggcgaggg tgccctcctt cgtgcccgac ccagggtgga
     4981 ccacgacgcc ggccggttta tccacgacca gaagagcgtc gtcctcatag aggacgtcga
     5041 aggggacgtc ctcgggttga agagagagct caaccgcggg gtgtggcgtt tcgatcaccg
     5101 acccggttcg cacccgatct ctcgggcgag ccgcgtttcc gtcgaggacc accccccggt
     5161 cgaccagctc tcttgcgacg aagcggctca ctccgagcag tgaagcaatg gccttgtcga
     5221 cgcgcatccc gtcgagcggc tccgggactt cgaatgtcag cgtgtccgcc acgactgaat
     5281 cagcaatagg accacggcaa tggtgatcga agtgtcggcc aggttgaacg tcggtatcca
     5341 ccacaaattg acccaatcga tcactttccc gtcgagaagg cctgggccgc gaaagatccg
     5401 atcgacgaga ttcccgagtg ccccacccat gatcaggccg aaggcgacga tttccagagt
     5461 ggggcgcgga tggcgaatgg cccagagcag gccaagcgtc accccaattg cagcaaggcc
     5521 caagaaaggg ccggcgcttt gcagcgaccc gaaggcagcg cctggattct cggtgtaggt
     5581 gaaagtcagg aatcctggga tcacctcgac ggggttaccc acgaagtgaa tcgaggccca
     5641 gcgtttggtc agggcgtcga tggtgacgac aaaggccgcg atcccggccg cgagcgcgta
     5701 ccggcgaatc aactcttgcg ggcgcagtcg acgcagagcg tcacataggg gaggacatcg
     5761 agccgagcca taggtatcgg cttgccgcag ctctcacaga tcccgtaccg cccgttctcg
     5821 atgttttcga gagcgcgctc caccttgccc aggagatcga ccgtgttctg ctctatggag
     5881 agctctttct cgtactcgaa cttcatcgac cctgcctcgg cattgccggg atcagggctg
     5941 cgatccgatg agctctcggt gagacgtgcc tcctccaatt cgagctggta atcccgaatc
     6001 atcaccagga ggcgctcacg ctcatcctcg agacgtttcc ggtaacgctc aaccgttgat
     6061 ttggcgagat ttctggccac gagtcatcct cgtaaagggc gggaacctta tcaattccct
     6121 cgttctactt cacagggggg cgcacaccct acgattcccg ctcccgagcg ccccgaaaga
     6181 ctggccgcat tgaccaacga gaccggattt caaagagtcg accccgcggt cgacatcccc
     6241 cgcctcgaag aagcggtgct cgccctgtgg cgtgacaccg acgccttcgc cgaatcggtg
     6301 cgccgacggc ccgccgataa ggagtacgta ttctacgacg gccctccatt tcccaccgga
     6361 agcccccact acggcaacct gctcgccggg gtgatcaagg acatggtccc ccgctactgg
     6421 acgatgaggg ggtatcgagt cgagcggcgc ttcggatggg acacccacgg gcttccgatc
     6481 gagatggagg tcgagaagca gctcggcatc tccggccccc gccagatcgc cgaatacggc
     6541 atcgaccgat acaacgaggc ctgccggatc ggagtccaag cgaacaccga ggcctgggag
     6601 acgctcaccg agcgcatcgg acgctgggtc gacttcgaaa acgactacaa gaccatggac
     6661 cccgacttca tggaaaccgt ctggtgggtg ttcaagcaac tctgggacaa agggctcatc
     6721 taccgcgact tcaaggtgct cccctactcg tggggcgcca ccaccccgct ctccaacttc
     6781 gaggcgaatc tcgactaccg ggatgttgag gacccctcga tcacagtgcg tctggcggtg
     6841 gtcgaaggaa acgacaaggc cgaggccggc gactatctgt tgatctggac gacgacgccg
     6901 tggactctgc cgggaaacct tgccgtggcg atcggcccag gcaacgagta cctgagaatc
     6961 gcagatgggg gcgaccacta ctggatcgct gctgaacggg tcgagtctgt gttcggcaac
     7021 cggggtgtga ccgtggcccg ggtcaagggc gccgaactcc taggggtcgg ctacgagcca
     7081 ccgttcgacc acttcgccga ccgttggcat gtcggagcct tccgcaccat cccctccgat
     7141 gaagtctccg tcgacgaggg gacggggctc gtgcacatgg cgcccgccta tggagaagcc
     7201 gactttctcg ctttgcaagc tgcaggtatc gaagccctgg tcgacccggt ggatgccgga
     7261 ggcaacttca ccgacgacgt gcccgaggtg gccggacgga acgtcaagga agcggacgcc
     7321 atcctcatcg acttgctgag gggctcgggc aagttggtcc actcggggcg catcgtccac
     7381 tcctatccct actgctaccg aacgggcact ccactcatct acaaggccat ccccacctgg
     7441 ttcgtggccg tggagcagtt tcgggaccgc atggtcgagc tcaacgagac ggtgcattgg
     7501 gtgcccgaat atgtcggttc ccgccgattc gggaattggc tggccaacgc ccgcgattgg
     7561 gcgatcagcc gcaatcgcta ctggggctcg tgcatcccgg tctggcagtg cgactcttgt
     7621 gaggacaggg tggccatcgg ctccgtggac gagcttgagg ggctctccgg acaacgaccc
     7681 accgacctgc accggcactt catcgacctg atcacctggc catgtgccag ctgcgacgga
     7741 gtcatgacca gggtccccga agtcctcgac tgctggttcg agtcgggctc gatgccctac
     7801 gggatgaatc actacccatt cgagaacacc gagcggttcg aagccacctt cccggcgaag
     7861 ttcatcgccg agggcctcga ccagacccgg ggctggttct acaccctgat cgtgctctcg
     7921 accgccctgt tcgaccggcc cgccttcgag aactgcgtcg tcaacggcct gatcctcgcc
     7981 gaggacggca gaaagatgtc caagagcctc aagaactacc ccgacccgtc ggagatcttc
     8041 gaccaacacg gcgcagacgc cgttcgcgcc tttctcatca attcgcccgt gcttcgagcc
     8101 gaaccgctgc ggttcagcaa ggatggagtg gccgacgtgg ttcggaccgt tctcctcccg
     8161 ctgtggaaca cctacagctt cttcaccacc tatgccgaag cggacggcct gaccacttcc
     8221 gacttcgccg acgcccctcc ggtcgaggag cgccccgaga tggatcgttg gattctctcc
     8281 gtgttgcaga gcctggtcgc ggaggtgaac acacagatgg agggctacta cctctacagc
     8341 gtggtgccgc cgatcctcgg attcatcgac cacctgacca actggtatgt gcgccgctcg
     8401 aggagacgat tctggagggc gaggggtgag tccggcgagg gcgatgtcga caagctggcc
     8461 gccttcgcaa ccctctatca ggtgttgacc acgttcatcg aagtcctggc gccgatcctt
     8521 cccttcatca ccgagcatct ctatcaggac ctcatcgccc gccacgatcc cgacgcggtg
     8581 cgaagcgtgc accaccgtga tttcccggag gcggacgaga ccctgatcga ctccgagttg
     8641 gaatcgtcga tggcggtggt cagggaggtc gtcaggctgg gacgcaacct gaggaagaag
     8701 gaagggcacc gtgtccgcca gccccttgct gggctcacca ttctcaccca cgaccccgac
     8761 atcggagccg ccgtggaaac gcacggggac atcatctccg aagagctaaa cgtgaagaag
     8821 gtgactgcga ccgacgacga aggggcgctc gttcacttgt cggcgaaggc caacttcagg
     8881 aagctcggcc cgactctcgg ctcagagatg cagaccgtcg ccgcgggaat cgccgcactt
     8941 ggaggcgacg acctcgcggg catcctcgac ggtggctcgg tcgaagttgc cggtcattcc
     9001 atcaatctcg atgacctgat cgtggagcgg acacccgttg agggcgtcat cgtggaaacc
     9061 ggcgccgact tcgcttgtgc gctggatacc accctcgatg atgacctggt gctggaaggc
     9121 gtggcacgtg agatcgtgag ccgggtgcag aagctgcgcc gcgaggccgg gctcgacgtt
     9181 gtcgatcgga tctcgctgac ctggcacagc ctggacccgc aggtggcggc agcgttcgac
     9241 acccatcacg atgtgattgc gggcgaagta ctcgctagtt cggtcgattc gtcgagcgcg
     9301 gaggtgggtg acagtttcga cctggatggc cgaacggtct ggttgtcact cgtccgtact
     9361 gactcgtagc gctcagtccg agttttcggc tttctcgacc ccgtcgacct cgtcggtcac
     9421 ttcgtcaccg gcttcttcga tgacttcctc taccccgtcc tcttcaacat cctccaccga
     9481 atcctcgaca gcgtcctctt cgatttcggc ctcttcgctc gccgcatcga gatccaattc
     9541 ctcggactcg tcgatcatgg cgtcggggtc ctcggcctct gcctcgtcgt cgaccgtggt
     9601 taggtcggtc gttagctcct cgtcggattc ggcgtcgtcc atctcatcat ctaacgccag
     9661 ttcgagatcc tctccgtcgt cgacggtttc agatgtcatg tcctcgaggt ctgccatgtc
     9721 gtcgacgtcg tcgtcctcgg cagcgaggtc ggcagactcg attgttgaat ccatctcgtc
     9781 gagcttgtcg gccactgacg tggcgagcag ggccagctgg ttcctgacac tggagacccg
     9841 aaccgtcagc tctgccatct cggtctgcgc cgccgtccgg cgctcctccc gttctgcctc
     9901 gaaggagtcg gcctcggtgc gggcctcggt caacgtccga tctgcttctc cccgtgcctc
     9961 ttcgagcaac cggtcggcct ccgtccgggc ttcgtccacg gtccgatcgg ccgcctgttg
    10021 ggccaggatc aagacccgac ccaccgaagc ctcgtcgccg acggcaacgc tggtggcgac
    10081 ggctgcaacc gcggggacct caggtccctt gctctgctcg agctcggcga tcttggcctt
    10141 ggcggctgtg agctcaccgt ggagatctct cagtgccacc acgatctcgt cgaggaagtc
    10201 gtccacctcg tcgaggtcgt agccgcgaag cgcggttgaa aaggtcttgt gctcgatatc
    10261 ggatggtgtg attggcatta atgaacctct cgagttggcg ttgaggatac gaaacgcgtc
    10321 cgccggctcc aagaatcagc cgacacgtct caggattccg atcaagatgt tgattccgat
    10381 caggagcact atcggagtca gatcgagcat tcccccaccc agccgaagcg gcgggatgac
    10441 ccggcgcaaa gggaggatca cggggtagat gatccggtcg agaaagatcg agacctttcc
    10501 cagcgggtga gtggatgaag tggggatcca ggagaggatg acccacgcga ggacggcgta
    10561 gaagtacacc tggaggatca ggatgatgat tccgatgggg tccaaggtca gtcgtcgaga
    10621 tcgtcgatct cgtaggctcc gagggtcgtc aatcggcgtt tttcgtcccg gctcaa
//
LOCUS       DBNHXU010000020         3358 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4569707, whole genome shotgun sequence.
ACCESSION   DBNHXU010000020 DBNHXU010000000
VERSION     DBNHXU010000020.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3358)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3358
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4569707"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..368
                     /locus_tag="AC161X_00610"
     CDS             <1..368
                     /locus_tag="AC161X_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019309140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA polymerase"
                     /protein_id="HIR4988079.1"
                     /translation="ARNQGYTTTLFGRRRYLPELKSDNFRIRQMGERMALNAPVQGTS
                     ADIIKKAMIELDQGLRSGGFRSSLILQIHDELILESPDEEMSAIEKLVVETMEGVVDL
                     DVPLVVDVGTGSDLASVKV"
     gene            471..2027
                     /gene="rpsA"
                     /locus_tag="AC161X_00615"
     CDS             471..2027
                     /gene="rpsA"
                     /locus_tag="AC161X_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HIR4988080.1"
                     /translation="MPNDQENAPDNVAEEAIEGAPDPTAEDLPEVEAPAVTEEPEAPA
                     AAESPVETEAEPVAPPEVSAPVDLALEDPTPEVPVPEVTASTSVSGREALVAPDLSDL
                     PDDIGDDFERAIAATVLEFKEGDIVEGTVVSVDGDGAMIDIGYKSEGLIPTRELSIRN
                     NVDPNDAVKVGDRVEAVVLNKEDDEGRLILSKKRAMYERAWGRIQEIADQEGTVEGTV
                     IEVVKGGLIVDIGLRGFLPASLVDLRRVRDLDPFIGQTITAKVIELDRPRNNVVLSRR
                     AYLEEAQAEERNAFLDGLGEGEVRDGVISSVVNFGAFVDLGGMDGLVHVSELSWQHVN
                     HPGELVKVGDKVTVKVLEVDRDRERISLSIRQTSDDPWDVFAREHEVGDVVDGVVTKT
                     VPFGAFVSVADGVEGLVHVSEIAVHRVESPELELSIGQPVKVKITEKENERRRVSLSI
                     KQASPDWQERETQIEEQKKAPAPSSRRRPQKEFEREFGPTDDERPPMPVDASLEAILQ
                     ELKERGIGRR"
     gene            2310..3233
                     /gene="cpaB"
                     /locus_tag="AC161X_00620"
     CDS             2310..3233
                     /gene="cpaB"
                     /locus_tag="AC161X_00620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HIR4988081.1"
                     /translation="MGRRAIVLLVALILAGLAAWAVWNYLQGIENDIEAGQEAVTVFR
                     AVDAIAEGTDGAIILSDFNAGGQLIVKGTEEKEDLPDLAITSEDQLNAVLSNKVSAGP
                     ISANSVLVETQWTEINIDVTPLAEQIPEGKQAMVISTDNVRGVNGFVNPGDRINLIIT
                     LDIAFDLLPEGFQGITVGAEAPPTEGAAPTEEATQVITYTRYVLQGIPVLATGRDIAT
                     DPDAPEQVEAEPTTPSDGTTETTVAEEDTGNETIFTLEVTPEQAERIAYAFENGSMWL
                     TLVPEDFVEVETQGVIIDTLFGGDLVEDIFG"
BASE COUNT          699 a         1009 c         1067 g          583 t
ORIGIN      
        1 tcgccaggaa tcagggctac acgacgaccc tcttcggccg ccgccggtat ctgcccgagt
       61 tgaagtcgga caacttccga atccgccaga tgggggagcg gatggcgctc aacgcaccgg
      121 tgcaagggac ttcggccgac atcatcaaga aggcgatgat cgagctcgac cagggcctgc
      181 gtagcggtgg ctttcgatcg tcactgatcc tccagatcca cgacgagttg atcctcgaat
      241 ctcccgacga ggagatgagc gccatcgaga agctggtggt ggagaccatg gagggagtgg
      301 tcgacctcga tgtgccgttg gtcgtagacg tcgggaccgg atcggatctg gcgagcgtca
      361 aggtgtgatt ctctcaatcc gcttggccat tctcccccga taggcgtaca cttcccgagc
      421 ctgcgggcct cgcgcacgca gagttccttt cggaggtaat cacccacaac atgcccaacg
      481 accaagagaa cgcgcccgat aacgtcgccg aagaggcgat cgagggggcg cccgacccga
      541 ccgctgaaga tctgcccgaa gtagaggcac cggcggtcac cgaggagcct gaggctcccg
      601 ccgcagccga gtcaccagtt gagaccgagg ccgagcctgt cgcgccgcct gaggtctcgg
      661 cacctgtaga tctggcgctc gaagatccga cacccgaggt cccggtacct gaagtcacgg
      721 cttcaacctc agtctccgga cgtgaggccc tggtcgctcc cgacctgagt gatctccccg
      781 acgacatcgg ggatgacttc gagagggcca ttgcggcgac cgtcctcgaa tttaaagagg
      841 gcgacattgt ggaaggaacg gttgtgagtg tcgatggcga cggcgccatg atcgacatcg
      901 gctacaagtc cgaagggctg attccgactc gcgagctgtc aatacgcaac aacgtcgacc
      961 ccaacgacgc cgtcaaggtg ggcgacaggg tcgaagccgt tgtcctcaac aaagaggacg
     1021 acgaagggcg tctcatcctc tccaagaagc gcgccatgta cgagcgcgcc tggggtcgga
     1081 tccaagagat cgccgatcaa gaaggcacag tcgagggaac tgtcatcgaa gtcgtcaagg
     1141 gcggtctcat cgtcgacatc ggcctccgtg gatttctccc ggcctcactc gtcgatctac
     1201 gccgggtccg tgatctcgac ccgttcatcg gccagacgat cacggccaag gtcatcgagc
     1261 tggaccgtcc gaggaacaac gtcgtcctct cccgccgtgc ctatctcgaa gaagcacagg
     1321 cagaggagcg gaacgcgttc ctcgacggcc tgggcgaggg cgaggtccgc gacggcgtca
     1381 tctcgtcggt ggtcaacttt ggcgcctttg tcgatcttgg tggcatggac ggtctcgtcc
     1441 acgtttcgga gctctcctgg cagcacgtga accacccggg cgagctggtc aaggtgggag
     1501 acaaggtcac ggtcaaggtc ctcgaggtcg accgtgatcg cgagcgcatc tcgctgtcga
     1561 tccgtcagac gagcgatgac ccctgggatg tcttcgcccg cgagcacgag gtcggagacg
     1621 ttgtcgacgg cgtcgtgacc aagaccgtcc cgttcggcgc attcgtctct gtcgccgacg
     1681 gagtggaagg cctggttcac gtatcggaga tcgcagtcca ccgcgtcgag tcgcccgagc
     1741 tcgagctctc gattggccag ccggtcaagg tgaagatcac cgagaaggag aacgagcggc
     1801 gccgggtctc gctttccatc aagcaagcgt caccggactg gcaggagcgg gagactcaga
     1861 tcgaagagca gaagaaggcc ccggctccga gcagccgtcg ccgtccgcag aaggagttcg
     1921 agcgcgagtt cgggcccact gacgacgaac gtcctcccat gccggtcgat gcctccctcg
     1981 aggcgatcct gcaggagctc aaggagcgtg gcatcggtcg gaggtgaccc gccggtcgaa
     2041 tccggcggtg gaagggtcat ctgactagtc acaacgtgat ggtttcacgg tgtgagctag
     2101 tcacgccttt caacccagtc acgatatgag acgaccgaac tatggagggt tgaggggaaa
     2161 ccctcaagac tcactccata cggtccgaat cattcctgtg gaattggact tgaaaaactg
     2221 ggagggcgcg ccccaaccgg tagcatgcca agcggtatgc gccggcgacg ggtcggcgcc
     2281 cgaccgaggg gacaaccgaa ggggactcaa tgggtaggag agccatcgtt ctgcttgtgg
     2341 ccttgatact ggccgggctc gccgcttggg cggtctggaa ctacttgcag ggcatagaga
     2401 acgacatcga agcaggccag gaggcggtca cggtcttccg tgccgttgac gccatcgctg
     2461 aagggaccga cggggcgatc atcctgtcgg acttcaacgc gggcggtcag ctcatcgtga
     2521 agggcaccga ggagaaggaa gatctccccg atttggccat cacttccgag gaccagctca
     2581 acgcagtcct gagcaacaag gtgtcggccg gtcctatctc ggcaaacagt gttttggtcg
     2641 agacgcagtg gacggagatc aacatcgacg tcacaccgct ggccgagcag atccccgagg
     2701 gcaagcaggc catggtgatc tcgacggaca acgtccgcgg ggtcaatggc tttgtcaacc
     2761 ccggcgatcg gatcaacctg atcatcaccc tcgacattgc gttcgacctt ctcccggagg
     2821 gattccaggg gatcacggtt ggcgctgagg cacctccgac cgaaggagcg gccccgaccg
     2881 aggaggcgac acaggtgatc acctacacca ggtacgtcct gcaagggatc ccggtgctgg
     2941 ctaccggtcg tgacatcgcg accgaccccg acgctccgga gcaggtggaa gccgagccga
     3001 cgaccccgtc tgacggcacc accgagacca cagtcgctga agaggacaca ggtaacgaga
     3061 ccatcttcac cctcgaagtc acacccgagc aggcagagcg gatcgcatac gccttcgaga
     3121 atggctcgat gtggctgacc ctcgttcccg aggacttcgt cgaagtggaa acacagggtg
     3181 tgatcatcga taccctcttc ggtggagacc tggtcgagga cattttcgga taagtttgag
     3241 taagaggttc gaccatgtca ttggctgagc acaacctgtt ggtggttgat aaggacgacg
     3301 acttcgtctc ggctgccaaa gagctgttcg aaggccgact gccggtagct cgctccat
//
LOCUS       DBNHXU010000021         1861 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3379716, whole genome shotgun sequence.
ACCESSION   DBNHXU010000021 DBNHXU010000000
VERSION     DBNHXU010000021.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1861)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1861
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3379716"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="AC161X_00625"
     CDS             complement(<1..992)
                     /locus_tag="AC161X_00625"
                     /inference="COORDINATES: protein motif:HMM:NF014877.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohexomutase domain-containing protein"
                     /protein_id="HIR4988082.1"
                     /translation="MDPRIVFKAYDIRGRTDNGEVTPDLFRRAGAAFVEVVGAREVAV
                     GRDCRVSSQGLFEGLVAGITGAGADVVDLGEVPTDLVYYYSGANVMPGAIITASHNPA
                     EYNGLKLCREMAAPVGADTGLNEIRDRVLSGFEPSSTERGSVKSHAAVDAYVDHLLSI
                     VEPSSIGQMRVAADGGNGMAGITVSRVFARLDSELDGLYLEPDGTFPNHPADPIQPEN
                     LEDLIAKVADGGHDLGVAFDGDADRAFFIDDAAHALSGSTVTALIARWMLQRHPGAPI
                     IHNLITSRAVPEIIAEAGGRAIRTRVGHSYIKQVMAESGAVFGGEHSGHYYFRD"
     gene            1054..1770
                     /locus_tag="AC161X_00630"
     CDS             1054..1770
                     /locus_tag="AC161X_00630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR4988083.1"
                     /translation="MRPTLSIVVPVYNEAAFIPQALPLLIAEMESLGLPYRIHLMENG
                     STDGSADIARAVAGDAPITVSSLATADYGNAMRQGFLEAEGDWVVNFDIDYFSANFLQ
                     AVMSTPDADLVIGSKRDPDSQDRRPWIRRLATLVFNLMLRTILGSKVSDTHGMKAFRH
                     NLVDDLAAIVVSRQDLYDTELVIRAERSGYRIVEVPVVVEEIRTARSSLMKRVPRTIV
                     GLLRIRRVLRTERSTQPSSA"
     gene            complement(1721..1825)
                     /locus_tag="AC161X_00635"
     CDS             complement(1721..1825)
                     /locus_tag="AC161X_00635"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HIR4988084.1"
                     /translation="MVISAVGIVVLIGAGVVGFRRKKAALSAQSEGPA"
BASE COUNT          359 a          597 c          547 g          358 t
ORIGIN      
        1 ttgtcacgga agtagtaatg gccgctgtgc tccccaccga acaccgcacc cgattcggcc
       61 atcacctgct tgatgtagct atgacccacc ctcgtcctga tcgctcgccc tcccgcctcg
      121 gcgatgatct cagggaccgc ccgtgatgtg atgaggttgt ggatgattgg agcacccgga
      181 tgccgttgca acatccatct ggcgatgaga gcggtgaccg tgctcccact cagagcgtgg
      241 gcggcgtcgt cgatgaagaa ggcccggtca gcatcaccgt cgaaggcgac acctaggtcg
      301 tgaccgccgt ccgcgacctt ggcgatcagg tcctcgagat tctcgggttg gatcggatcg
      361 gccgggtgat tggggaaggt cccatcaggc tccagataga gaccgtccag ttcggagtcg
      421 agtctggcga acactcggga aacagtgatc cccgccatac cgttgccgcc atcggcggca
      481 acccgcatct gccctatgga agatggctcg actattgaga gcagatgatc gacgtaggcg
      541 tccaccgccg catggctttt gacgctcccc ctttccgtcg acgagggctc gaatccggag
      601 agcacgcgat ccctgatctc attcagaccg gtgtccgccc caaccggcgc ggccatctcg
      661 cggcatagtt tgagcccgtt gtactcggcg gggttgtgag aggccgtgat gatggcgccg
      721 ggcatgacgt tggctccgga gtagtaatag acgagatcgg ttgggacctc tccgaggtcg
      781 acgacgtcgg caccggctcc tgtgatcccg gccaccaggc cttcgaacag cccctgggaa
      841 gagacacggc agtcacggcc cactgctacc tccctggcgc cgaccacctc tacgaaagct
      901 gcgccagccc tccgaaacag atctggcgtc acctctccgt tgtcggtgcg acctcgaatg
      961 tcataggcct tgaaaacgat tcgggggtcc atcgggacca tcgagtgtag gggtgggtct
     1021 atccgtcacc gattcctaca ctcgcccact ccgatgcgcc ccaccctctc aatcgtcgtg
     1081 cccgtctaca acgaggcggc cttcatcccc caggcgttgc ccctgctcat cgcggagatg
     1141 gagagcctcg gcttgcccta tcgcatccac ctcatggaga acggatcgac cgacggatca
     1201 gcggacatcg cacgagccgt cgccggagac gcgccgatca cggtgtcctc cctggcgacg
     1261 gccgactacg ggaatgcgat gagacagggt tttctggaag ctgagggtga ctgggtcgtc
     1321 aatttcgaca tcgactactt ctctgccaac tttctccagg cagtgatgtc gacaccggac
     1381 gcggatctcg tgatcggatc gaagagagac cccgattcac aggaccggcg accgtggata
     1441 cgtcgactcg cgacgcttgt cttcaatctc atgctgcgaa cgatcctggg gtcgaaggtc
     1501 tcggacaccc acggcatgaa agcgtttcgt cacaacctgg tcgacgacct cgctgcgatt
     1561 gttgtcagtc gccaggacct ctacgacaca gagctggtga tacgcgccga gcgcagcggc
     1621 tatcgaatcg tcgaagtccc ggtcgttgtt gaagaaatca ggacggcaag atcgtctctc
     1681 atgaaacgag tcccacggac catcgtcggg ctgttgagaa tcaggcgggt ccttcggact
     1741 gagcgctcaa cgcagccttc ttccgcctga agccgaccac gccggcgccg atcagcacaa
     1801 caatcccaac tgccgagatg accatgcccc cggtctcggc ccaggtgttc ttgaactcga
     1861 g
//
LOCUS       DBNHXU010000022         2606 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3975186, whole genome shotgun sequence.
ACCESSION   DBNHXU010000022 DBNHXU010000000
VERSION     DBNHXU010000022.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2606)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2606)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2606
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3975186"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1136
                     /locus_tag="AC161X_00640"
     CDS             <1..1136
                     /locus_tag="AC161X_00640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988085.1"
                     /translation="LPGTENLTPEVRDELVNLVAVTEEVRGLQFIEMPTITVVSEEEL
                     EERVRAQFEEDAADFPADAALYKLLGLLGGDVDFLTLLTDVYGEQVAGYYDGDTRELV
                     VPITTEGFSVVQRATLVHELTHALTDQQFEFHAVFDAMFEEDRLDQASAYQALIEGDA
                     SLAQLHYLQSLSQAELGEFFAEALDVDTTALDAAPQFLQDSLIFPYDSGLAFVQELYD
                     NDGDWSDVNDAYTSMPSLPGSTEQVITPSDFERDLPKEITPEVVSVPGYDLERTSVWG
                     ELGFRVMLDQVLGQGVGVTAADGWGGDYYLQWFDGTNAALLLVYEGDTNRDTDELRQA
                     MSDYAAAVFEQDYASVQVIDGQLVFIAADEEAVGQLILEAISA"
     gene            1181..1498
                     /locus_tag="AC161X_00645"
     CDS             1181..1498
                     /locus_tag="AC161X_00645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR4988086.1"
                     /translation="MRRVRLVSDGWASEASRPATFVERFRGLRGQPVDARLLFETSSI
                     HTIGMHRSIALVMIGADWRVVRVQTLAPNRLVRERAVRFILELPEGAEVPSEGDDVVI
                     TDV"
     gene            1491..2330
                     /gene="rsmI"
                     /locus_tag="AC161X_00650"
     CDS             1491..2330
                     /gene="rsmI"
                     /locus_tag="AC161X_00650"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: protein motif:HMM:TIGR00096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HIR4988087.1"
                     /translation="MSDRGRLVLCATPIGNLADVSDRLRTALAEADVVYAEDTRRTSV
                     LLAHVGVSTPIRSLFVGNEEARTEELVGAVLEGKVVALVSDAGMPGISDPGASAVTRV
                     REAGRPVTVIPGPSAVTSAVAVAGFGGEGFVFEGFLPRRGKARSQRLQRIAVEDRPVV
                     LFLAPHRFEADLVDLASTSGGERRLVVTRELTKLHEEVWAGTISEARDEWSQRAPKGE
                     FTIVLAPAEPPVISFSEAVEQARLLVSGGSTPSEASREIGRTTGLPRRQIYEALIGSH
                     DRS"
     gene            complement(2315..>2606)
                     /locus_tag="AC161X_00655"
     CDS             complement(2315..>2606)
                     /locus_tag="AC161X_00655"
                     /inference="COORDINATES: protein motif:HMM:NF015947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR4988088.1"
                     /translation="ATDRRPRGRLGSRRSMPGMETGIARKIDDLGRIVIPAETRRLFN
                     IREGDHLFIAVEGSSIVLRKMTDTCTFCGSDTKVTQFKGRGVCSSCRSQLRS"
BASE COUNT          451 a          787 c          859 g          509 t
ORIGIN      
        1 ctcttcccgg taccgagaac ctgacccccg aggtgcgaga cgagttggtg aacctggtcg
       61 cggtcaccga ggaggtgcgc gggctccagt tcatcgagat gccaaccatc actgtcgtgt
      121 ccgaggaaga gctcgaggag cgggttcgtg cccagttcga agaggatgcg gccgactttc
      181 ccgctgatgc cgcgctctac aaattgctag ggctcctcgg cggcgatgtg gactttctga
      241 ccttgctgac cgatgtctac ggggagcagg ttgccggcta ctacgacgga gacacccgcg
      301 agctcgtggt gccgatcacg actgagggtt tcagcgtcgt ccagcgggca acgctggttc
      361 acgagttgac gcatgcgttg accgatcagc agttcgaatt tcacgcggtc ttcgacgcga
      421 tgttcgaaga ggaccggctc gatcaggcct ccgcttatca ggcgctcatc gaaggcgacg
      481 ccagcctggc ccagcttcat tatttgcagt ccctatccca ggccgagctg ggtgagtttt
      541 tcgccgaggc cctcgatgtc gacaccacag ctctcgacgc tgcgcctcag tttctccagg
      601 actcgttgat ctttccctac gactccggtc tggcgttcgt ccaggagctg tacgacaacg
      661 atggtgactg gagtgacgtc aacgacgcat acacgtcgat gccttcgctt cccggttcga
      721 ccgagcaagt gatcacaccg agtgacttcg agcgtgacct gccgaaagag atcacgcccg
      781 aggtggtctc ggttcccggc tacgacctgg agcggacgtc ggtgtgggga gagttggggt
      841 tccgagtcat gctcgatcag gtgctgggcc agggggttgg tgtcaccgcg gccgacggat
      901 ggggcggcga ctactacttg cagtggttcg acggcaccaa tgccgccctg ctcctcgtct
      961 acgagggaga taccaaccgt gacaccgacg agttgcgtca agcaatgtcc gactacgcgg
     1021 cggcagtgtt cgagcaggac tacgcctcgg ttcaggtcat cgacgggcag ctggtgttca
     1081 tcgctgccga cgaagaggcg gttggccagc tcatcctcga ggcgatctcc gcctaaggcc
     1141 acccttcccg gccggcaccg catcatctag cgtcggtctg atgaggaggg ttcgactggt
     1201 cagcgacggt tgggcgtccg aggcgtcccg gcccgcgacc ttcgtcgaga gatttcgcgg
     1261 acttcgcggg caacccgttg atgcacggct ccttttcgaa acctcgagca tccacacgat
     1321 cggcatgcac cgatcgatcg cactggtgat gatcggagct gattggcggg tggtgagggt
     1381 gcagactttg gcgccgaacc gtctggtgcg agagcgggct gttcgcttca tcctcgagct
     1441 cccggagggc gccgaggtgc catctgaggg agatgacgtc gtgatcaccg atgtctgacc
     1501 gggggcgtct tgttctctgt gccactccca tcgggaacct ggccgatgtc tcggatcggt
     1561 tgcgaaccgc actcgctgag gccgacgtcg tctatgccga ggacacaaga aggacttccg
     1621 tgctcttggc gcatgtgggg gtatcgacgc cgattcgatc gttgttcgtg ggcaacgagg
     1681 aggcgcgtac cgaggagctc gtgggcgccg tcctcgaggg gaaggtcgtc gccctggtca
     1741 gcgacgccgg catgcccgga atctccgacc ctggagcatc agccgtgacg agggtcaggg
     1801 aggccgggcg cccggtcaca gtgatacccg gcccgagtgc ggtgaccagt gctgtcgccg
     1861 tcgccggatt tggcggcgag ggatttgtgt tcgagggttt tctcccccga aggggaaagg
     1921 ccaggtcgca gcgtctccag aggatcgcgg tcgaggatcg acccgtggtc ctgttcctag
     1981 ccccgcaccg gttcgaggcc gatctcgtgg atctcgcttc tacctccggg ggggaacgtc
     2041 ggctggtcgt caccagagag ctcaccaagc ttcacgagga agtgtgggcg gggaccatca
     2101 gcgaagcgcg cgatgaatgg tcacaacgcg ccccaaaagg cgagttcacc atcgttcttg
     2161 ccccggccga gccgccggtc atcagcttta gcgaggcagt cgagcaggct cgattgctgg
     2221 tttcaggagg atcgacgccg tcggaggcat cccgtgagat cggccgcacc accggcctgc
     2281 ctcgccgcca gatctatgaa gccctgatcg ggagtcacga ccggagctga gaccggcagg
     2341 aggagcagac accacgaccc ttgaactggg tgaccttggt gtccgatccg cagaaggtgc
     2401 aggtgtcggt catctttcgg agcacgatcg aacttccctc gaccgcgatg aaaaggtggt
     2461 cgccctccct gatattgaac agacggcggg tctccgcggg aatcacgatc cttcccaggt
     2521 cgtcgatctt gcgggcgatt ccagtttcca tgccgggcat tgacctccgg gatccgagcc
     2581 taccccgggg cctccgatcg gtggcg
//
LOCUS       DBNHXU010000023         2208 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5364913, whole genome shotgun sequence.
ACCESSION   DBNHXU010000023 DBNHXU010000000
VERSION     DBNHXU010000023.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5364913"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1133)
                     /locus_tag="AC161X_00660"
     CDS             complement(<1..1133)
                     /locus_tag="AC161X_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR4988089.1"
                     /translation="MIDWLARHDPKRVFLETPFGSHTYGDVLESLHNRPVAGTEVLRP
                     RLTAGSVVDILAVMSLGSAVIVGPDADAPGDLDPGGAATVVFTSGSTGRSKGVRLTRD
                     NWEAAVVASQVHLGNSPDDVWLLGMGMHRVGGIAIVLRSARTGGTVRMPAKFDAAEFA
                     GGLRSGVTIASLVPTMLHRMLDIDPGPYRGVRAVLVGGGPIPDGLLERAVAAGLPVLP
                     SYGMTETCGQVATLRPGSAIEKKVHPLPGVEVRIEPDQRIAVRGRMVSPGYVGEPDRL
                     SAGWFLTGDLGALDTDGALRVLGRADDVIVSGGVNIDPGVIEAALDEIAGVESALVFG
                     MPSPEWGMEVACVYVGSLVPEDVEVRLRERLSGPLIPKRWHRV"
     gene            1165..2064
                     /locus_tag="AC161X_00665"
     CDS             1165..2064
                     /locus_tag="AC161X_00665"
                     /EC_number="4.1.3.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019634597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008935 - 1,4-dihydroxy-2-naphthoyl-CoA
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoyl-CoA synthase"
                     /protein_id="HIR4988090.1"
                     /translation="MVSDIFDSADWDSVPGFDLADITYHRAKGAGVVRIAFDRPEVRN
                     AFRPQTVDELFATLDHARQTTDVGCVLLTGNGPSPKDGVWAFSSGGDQRIRGADGYKY
                     ADGEEKESVDPARVGRLHILEVQRLIRFMPKVVIAVVPGWAVGGGHSLHVVCDLTIAS
                     EEHALFKQTDADVASYDAGYGSAYLARQVGQKRAREVFFLGRTYTAQEAFEMGMVNAV
                     IPHADLERVALEWGVEITHKSPTSQRMLKFAFNLIDDGLVGQQIFAGEATRLGYMTEE
                     AREGRDAFLEKRDPDFGRYPWHF"
BASE COUNT          419 a          731 c          670 g          388 t
ORIGIN      
        1 tcgacccgat gccagcgctt cgggatcaga gggccggaca accgctctct gagccggacc
       61 tcgacgtcct caggcaccaa actccccacg tagacacagg cgacttccat cccccattcc
      121 ggactgggca tcccgaacac cagcgccgac tcgactcctg cgatctcatc cagggcagcc
      181 tcgatcaccc cgggatcaat gttcaccccg ccgctgacga tgacatcgtc tgcccggccg
      241 aggacccgca gcgccccgtc ggtatccaac gctcccagat cgccggttag gaaccatcca
      301 gccgacagac ggtcgggctc accgacatac cccggagaga ccatccgccc ccgcaccgcg
      361 atccgttgat cgggctcgat gcgcacttca acgccaggca agggatgcac cttcttctcg
      421 atagctgacc ccggacgcag ggttgcgacc tggccacagg tctcggtcat cccgtagctg
      481 ggaaggacgg gcagtcctgc cgcgacggcc cgctccaaca atccgtccgg gatcgggcca
      541 cccccaacca gtaccgctcg caccccgcgg taggggccgg ggtcgatgtc gagcattcga
      601 tggagcatcg tggggacgag agaggcaatg gtcaccccgc ttcgtaggcc cccagcgaac
      661 tcagcggcat cgaacttcgc aggcattcgc accgttccgc ctgtgcgagc cgagcgaagg
      721 acgatggcaa tgcctcccac tcgatgcatc cccatcccaa gcagccaaac gtcgtcagga
      781 ctgttcccca gatgcacctg agatgcgacc acggccgcct cccaattgtc acgggttagc
      841 cggaccccct tcgaccttcc agtcgaaccc gaggtgaaga ccaccgtggc cgcacccccg
      901 ggatcgaggt cccccggagc atcggcgtca ggaccgacga tgactgcact gccaagagac
      961 atcacggcca ggatgtccac aactgaaccg gccgtgaggc gaggacgcag aacttctgtg
     1021 ccggctaccg gtcggttgtg aagtgactcg agaacatcgc cgtaggtatg agagccaaac
     1081 ggcgtctcca aaaacacccg tttcgggtcg tgacgggcga gccaatcgat cacatgacga
     1141 ttgtgcctct tatcctgcga gtccgtggtt tccgatatct tcgattcagc cgactgggac
     1201 tcagtgcccg ggttcgacct cgccgatatc acctatcacc gggccaaggg cgccggggtg
     1261 gttcgcattg ccttcgaccg gcccgaagtc cgaaatgcct ttcgccccca gaccgtcgac
     1321 gagctcttcg ccacgctcga ccacgctcgt cagaccaccg atgtcggctg cgtcctgctc
     1381 accggaaacg ggccatcacc aaaagacggc gtttgggcgt tctcgtctgg tggggaccaa
     1441 agaatccgcg gcgccgacgg ctacaagtac gcggatgggg aggagaagga aagcgtggac
     1501 ccggcgcggg tcggccgtct ccacatcctc gaggtgcagc gactgatccg tttcatgcca
     1561 aaggttgtca tcgctgtggt gcctggttgg gctgtgggtg gcggtcacag ccttcacgtc
     1621 gtgtgcgacc tcacgatcgc gtccgaggaa catgcgcttt tcaagcagac cgatgccgac
     1681 gttgcctcgt atgacgccgg ctatggctcg gcttacctgg cgcggcaggt aggacagaaa
     1741 cgggctcgtg aagtgttctt cctgggaagg acctataccg cgcaggaggc gtttgagatg
     1801 gggatggtca acgcggtgat cccccacgcc gacctggaaa gggtggccct ggagtgggga
     1861 gtcgagatca ctcacaagag ccctacgtcg cagcggatgc tcaagtttgc gttcaacctc
     1921 atcgatgacg ggttggtcgg tcaacagata ttcgccgggg aagccacccg cctcggatac
     1981 atgaccgagg aggctcgcga gggccgcgat gccttcctcg aaaagcgcga tccggacttt
     2041 ggccgctatc cctggcactt ctgatccctt gactcgtttc caggcctgga aggccgcagc
     2101 gcgggtgcac acgttgcccg cagcggtggt gccggttctg gttggaggtg gcctcgctct
     2161 tggggaggga gtgtttcgtg ccgacgcgtt cagcctgaca ttgatcgc
//
LOCUS       DBNHXU010000024         9380 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5166503, whole genome shotgun sequence.
ACCESSION   DBNHXU010000024 DBNHXU010000000
VERSION     DBNHXU010000024.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9380)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9380)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9380
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5166503"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1203
                     /locus_tag="AC161X_00670"
     CDS             <1..1203
                     /locus_tag="AC161X_00670"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4988091.1"
                     /translation="RASVQLHNADEAGRAATVARSHIALAALGSEDQQSLSAQEATNA
                     LSAFDDSIASLEGAPDAEDAIAGGKAFAAAARAAVGSIGTGEINQEDVDLRFRELISS
                     LDGLRLGLVAYLDLMGSAYSVLGIVLGIATVGLAPLVLVIWTRSMARKTVEVRELSLQ
                     LKHEGEIRDAHNSVLRTVVHEFRTPLTGISGLAAVLEDPSLRASAEAAEMIGMIRTEA
                     EDMSQLTDDILASAGLESGQLEIRVEPIDVAEVADRALAMFHRRGIAIDVNLDPAAVI
                     ADEFRLRQVLKNLISNAVKYGGPRISVTGTETLDTYELVVADNGAGVPDAIVDQLFEP
                     FPHGGKGAPSQSVGLGLFIVRELAEAMGGSVVYERTDDITTFTVSLPLVEAESAQIIL
                     HPAMSKGA"
     gene            complement(1306..1623)
                     /locus_tag="AC161X_00675"
     CDS             complement(1306..1623)
                     /locus_tag="AC161X_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007976943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988092.1"
                     /translation="MSARVVVVVGGSVVVVVGGGGGGLACFTGGWEVEVVVVAAVVVV
                     VAGGALVVVVTALVVVVVGGAVVVVVGGAVVVVVGGGGGAVVVVVVVLPLHQSFHQPH
                     RWL"
     gene            1664..1870
                     /locus_tag="AC161X_00680"
     CDS             1664..1870
                     /locus_tag="AC161X_00680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988093.1"
                     /translation="MPDQVSAATETGSGIQLLVSLAGAFVGMTVQGLGYYLFVFLVPV
                     LVAIWLTVVRRREDQEPGLLHETP"
     gene            complement(1890..1964)
                     /locus_tag="AC161X_00685"
     tRNA            complement(1890..1964)
                     /locus_tag="AC161X_00685"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1927..1929),aa:Ile,seq:gat)
     gene            2044..2116
                     /locus_tag="AC161X_00690"
     tRNA            2044..2116
                     /locus_tag="AC161X_00690"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2077..2079,aa:Ala,seq:tgc)
     gene            2189..3367
                     /locus_tag="AC161X_00695"
     CDS             2189..3367
                     /locus_tag="AC161X_00695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011957265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR4988094.1"
                     /translation="MPNAVIIDAVRTPIGRRNGVFKDIHPVDLAAIPLQALVERNQLD
                     PSQIDDVIMGTVSQTGEQAYNVGRNAALAAGFPEEVPGVTIDRQCGSSQQAAHFAAQA
                     VMAGVQDIVIAAGVESMTRVPMGVTAQQGPGLPFGPRMLERYSQGLVPQGISAEMIAE
                     KWGLSRTDLDEIALESHRRAGRATEEGRFEEQIIPVGITRENGNRETVTRDEGIRSDS
                     SLEKLAGLQPAFKGDGVVTAGNSSQISDGAAAVLVMSEDRAAQLGLTPKARFVSFAMA
                     GVDPIMMLSGPIPATEKVLARAGLEVGDIDLFEVNEAFASVVAAWRVEHGGSDPISLW
                     DRTNVNGGAIALGHPLGASGARVLTTMLWELERTGGRYGLQTMCEGGGMANALIIERL
                     "
     gene            3381..3629
                     /locus_tag="AC161X_00700"
     CDS             3381..3629
                     /locus_tag="AC161X_00700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988095.1"
                     /translation="MTPLNVVLAVALGLIIWRVSISVIRMLSTPPPEIDPADVVAVDQ
                     DYKCTICGAELTMRAINVQDEAPPRHCREDMIPVWRPA"
     gene            3721..3960
                     /locus_tag="AC161X_00705"
     CDS             3721..3960
                     /locus_tag="AC161X_00705"
                     /inference="COORDINATES: protein motif:HMM:NF018487.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein CrgA"
                     /protein_id="HIR4988096.1"
                     /translation="MPESKRRKPKSKPLPTQSAAAAAKAKPPSPTWYVAVMAGLMTIG
                     VLLVLSRFVFELDRLVLIGGLACIAIGFLMTTNYR"
     gene            complement(3962..5893)
                     /gene="pknB"
                     /locus_tag="AC161X_00710"
     CDS             complement(3962..5893)
                     /gene="pknB"
                     /locus_tag="AC161X_00710"
                     /inference="COORDINATES: protein motif:HMM:NF033483.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HIR4988097.1"
                     /translation="MNTPSELTERYHITSHLARGGMADVYEGQDTLLNRKVAIKVLHA
                     QYSADEAFVKRFRREAQAAANLSHPNIVGIYDWGQAQGTYFIVMELVDGRSLRDVLKS
                     EGALLARRATEIASEVAAALSIAHQAGLVHRDVKPGNILLAKDGTVKVTDFGIARAWD
                     DSAELTKTGAVIGTATYFSPEQAQGAAADARSDVYALGVVLYEMLTGRPPFTGDSPMS
                     VAFQHVSTEASAPSALNADVPPALDTVVGKAMRKDPNGRYQSADEMRADLVAVLRGQN
                     VEPAVVGAVVAGAAAGDNATRVMTAQVPPATVPPDEVYRGVEEEPSSQMPFIVTAFAL
                     LIALGILLFVLFRLAGGGNISNDLVEVPNVAGLTRQGAEARLITAGFTVKFTAEASQD
                     VEVDLVIRTNPAGGTEAEPDSEVEVFYSTGAEEFEVPPLIGQTQEAAERIIEQNGFVL
                     GEVVEEPSAEFAEGTVIRQDPNSGVLAGRGAAISITVSTGAETHILPVLEGETERDAV
                     TALQRLGLLSTVNDEFSNTVDEGLVIRTEPEAGAEVRSGDTILLVVSQGPRPVEVPNL
                     LGSTQGQAEGILADLGLEMNVSASTQPVVDASQDGRVVAQFPDDGVEVFPGDVVTVTL
                     GKFTPPPTTTTTTATPPST"
     gene            complement(5931..7388)
                     /locus_tag="AC161X_00715"
     CDS             complement(5931..7388)
                     /locus_tag="AC161X_00715"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR4988098.1"
                     /translation="MNGPVRRISIGVFTSFFVLLLAVTWIQVIHADALKADPRNARPA
                     LSERGKERGLIVTIDGTVVAESVADPDDPRSFVRTYPEGEAFAHVVGYNSFIVGDSGL
                     EEAYAPVLRSKRDLTISDLISVILGRDLRPHSIEMTVNGPLQMAAYEALGGQTGAVVA
                     IDPKTGAVLAAVSSPSFDPESLLGEDAAAVWDELLADPVSPLSDRSTKELFAPGSTFK
                     TVVTATALDTGEADPSTEFPDPVEYQLEGSTETISNFDGEVCNDGESVTLLVAFVRSC
                     NTVFADLSVRLGARDIGITAEALGFNTDLDFPWSVPQASYPVDELIDDPAALAQSGIG
                     ERDVRATPLHMAMVAAAVANQGEVFNPYLVNRVFDADGSTLETSQPISLGRAMAPATA
                     SVLAQMMERVVTEGTGRNAAVPGIRVAGKTGTAEGAGDGPHAWFIGFAPVDDPTIAIA
                     VLIAGGGDIGESATGGGVAAPIAADLISLWLQGTP"
     gene            complement(7385..8680)
                     /locus_tag="AC161X_00720"
     CDS             complement(7385..8680)
                     /locus_tag="AC161X_00720"
                     /inference="COORDINATES: protein motif:HMM:NF013279.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0015648 - lipid-linked peptidoglycan
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HIR4988099.1"
                     /translation="MTRNAEGATVVAASVLAAFGITLVNFAREQSVDAQTALTFLVFL
                     IAFGGLHLAVRTWAPRATPLLIAPITALTAIGFIEIYRLDPVKAGLQRWWMLIASAAA
                     IAVLALFARSSTTILRRYRNITLLVSFILLALPNLPTDWGFPLKGLVVNGSRLWIVLD
                     LEFTTLQFQPAELAKLGFVIYVAAYLADHQKALREARRHLGPIRFPEPRQLIPLVITW
                     MVSILILVSQRDLGASLLLFAGFVLLLYAATGNTGYLVTGGFMAAAAGVASYFMFEHV
                     QRRVVAWLAPFEHFDDEGYQIAQGLFGMGTGSLSGAGPGLGRPDLIPNASTDFIFAAV
                     GEELGFAGTVSVLALFSLVIAVGLGISFRSRDMFRKLLAAGLTFVFAVQTFLIIGGVV
                     RVVPLTGITLPFMSYGGSALVGNLILIALLLRISHEEAT"
     gene            complement(8670..>9380)
                     /locus_tag="AC161X_00725"
     CDS             complement(8670..>9380)
                     /locus_tag="AC161X_00725"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR4988100.1"
                     /translation="WAAATHQGMARTNNEDTIFPETSGESTGPAVLMVADGMGGHIAG
                     EVASRLAVNTAASADLGPADRVAAANRAIREQVARQPDLEGMATTLTLVELTANGTAH
                     FGHIGDSRAYIYRAGELRQLTDDHTVVAQYIAAGTLSPEEAADHPHSHMLTRCLGLTR
                     FVNVDEIELPLENGDRLLLCSDGLTLMLTDDEIAENLGTESADETVWALVEQSNQAGG
                     HDNISVIIVDVLPGANDT"
BASE COUNT         1710 a         2951 c         3032 g         1687 t
ORIGIN      
        1 cgagcttcgg tccagctgca caacgcagat gaggccggcc gtgcggcgac ggtggcccga
       61 tcccacatcg cactggcggc cttgggatcg gaagatcagc agagcctttc cgcccaggag
      121 gcaaccaacg ccctctcggc ctttgacgac tccattgcca gtctcgaagg ggctccggac
      181 gccgaggacg ccatcgccgg cggtaaggct tttgcggcag ctgccagagc ggcggtgggc
      241 tccattggga cgggagagat caatcaggaa gacgtcgacc tccgattccg ggaattgatt
      301 tcctccctcg acgggttgcg gctcggtttg gtggcctacc tcgatttgat ggggtcggcc
      361 tacagcgtgc tcggaatcgt gctcggaatc gccaccgtgg gccttgcacc gctggtcctc
      421 gtcatctgga cccggtctat ggcgcgcaag acggttgaag tgagggagct ctccctccag
      481 ttgaagcatg agggggagat ccgcgatgcc cacaactccg tgttgcggac cgtggtgcat
      541 gagttccgta ctccgctcac cggtatcagc ggtttggccg cagttctgga agacccatct
      601 ctacgggcgt cggccgaggc tgcggagatg atcggcatga tccgcaccga agccgaggac
      661 atgtcgcagt tgaccgatga catcctggcc tcggcggggc tggagagcgg tcagctcgag
      721 atccgggtcg agccgataga tgtagccgag gttgcggatc gagccctcgc catgttccac
      781 cggcgtggga tcgccatcga tgtgaatctg gacccggcgg cggtaatcgc ggatgagttc
      841 cggctccgcc aggtcctgaa gaacctcatt tccaacgcgg tgaagtacgg ggggccaagg
      901 atcagcgtca ccggcaccga gactcttgac acctacgagc tggtggtggc ggacaacgga
      961 gccggagttc cggacgcgat cgtcgatcag ctcttcgaac ccttccccca cggcgggaag
     1021 ggcgctccca gccagagcgt cggtctcggg ttgttcatcg tccgtgagct cgccgaagcg
     1081 atgggcggct cagtcgttta cgagcggaca gacgacatca ctaccttcac cgtttcgctt
     1141 ccccttgtgg aggcggagag cgcacagatc atcctccacc ccgcgatgag caagggcgct
     1201 tgagttgacc cgtcgccgtg tcaccgccat cggcgcgggc cttctggtgc tggggcttgt
     1261 cggttcggcc gccccgtcgg catcggcagc aactcagacg gcttcttaca gccaccggtg
     1321 cggctggtgg aacgactggt gcaatggcag caccactacg accacgacca ccgctccgcc
     1381 tccaccaccg actacgacga ctactgcccc acccacaacc acgacgactg ccccacccac
     1441 gacgacaact accaaggcag tcacgaccac aaccaaggca ccgccagcga ctaccaccac
     1501 gactgcggcg acgaccacga cctcgacctc ccagcctccg gtgaagcacg ccagcccacc
     1561 gcccccacct ccaaccacga ctacaacgct ccctccgacg accacgacca ctcgggcaga
     1621 cacagtgggg ttctttgccg acttcgatcc cccgagaggc tccttgccgg atcaggtatc
     1681 ggctgcgact gagaccggct caggcatcca actgctggtc tcgcttgccg gcgcatttgt
     1741 cggaatgacg gtgcagggcc tcggctatta cttgtttgtc ttcttggtgc cggtcttggt
     1801 cgccatctgg ctgaccgtgg tcagacggcg cgaggaccag gaaccgggct tgcttcacga
     1861 aaccccgtaa ttcgaagggg tttatggggt gggcccacgt ggacttgaac cacggacctc
     1921 atccttatca gggatgcgct ctaaccgggc tgagctatgg gcccccggca gccaagggct
     1981 gcagcgagcg ccaaatctta tcgggtcgtc tgagtcaggc gaacccgtaa actggcatct
     2041 ccaggggacg tagctcagct ggaagagcac cggctttgca agccgggggt cgtgggttcg
     2101 agtcccatcg tctccaccgg atcgccgtac gggattgatt ccatcccggt cggtgttctc
     2161 ccttccgcga cgtgaccgag gagcccacat gccgaatgcc gtgatcatcg atgcggtgcg
     2221 gacgcccatc gggaggcgga acggtgtctt caaggacatt catcccgtcg acctggccgc
     2281 cattcccctg caggctctcg ttgaacgcaa ccaactcgac ccctcgcaga tcgacgacgt
     2341 gatcatgggc acggtcagcc aaaccggaga acaggcctac aacgttggcc gaaacgccgc
     2401 cctcgccgcc gggttccccg aggaggtgcc cggggtgacc atcgaccgac aatgcggctc
     2461 ctcgcagcag gccgcccatt tcgcagccca ggcagtcatg gctggggtgc aggacatcgt
     2521 gatcgccgca ggcgtggaga gcatgaccag ggtgccaatg ggagtgacgg cccagcaggg
     2581 ccccggactg ccgttcgggc ctcgaatgct ggagcggtat tcgcagggat tggtccctca
     2641 gggcatctcg gcggagatga tcgccgagaa gtggggccta tcccgcaccg acctcgacga
     2701 gatcgctctc gagtcacacc gtcgggcggg ccgtgccact gaggagggcc gattcgaaga
     2761 gcagatcatc cccgttggga tcacacggga gaacgggaac agagagacgg tcacccgcga
     2821 tgagggaatc cgctccgatt catccctcga gaagctggcg ggcctccagc cggccttcaa
     2881 gggcgatggc gtggtcaccg cagggaactc atcgcagatc tccgacggtg cggcagcggt
     2941 gctggtcatg tctgaggacc gggccgccca actcggcctt actccgaaag cccgttttgt
     3001 ctccttcgcc atggccggtg tcgatccgat catgatgctc tccggtccta tccccgccac
     3061 ggagaaagtg ctggcccgag ccgggctcga ggttggagac atcgacttgt tcgaggtgaa
     3121 tgaggcgttt gcatcagtgg tcgcggcctg gagggtcgag catgggggat cggacccgat
     3181 ctccctgtgg gatcggacca atgtgaatgg cggcgcgatc gctctcgggc atcccctcgg
     3241 tgcttccggc gcccgtgttc tgaccaccat gttgtgggag ttggagcgga ccggtggtcg
     3301 ttacggcctg cagacgatgt gcgagggcgg gggcatggcc aacgctttga tcatcgagcg
     3361 actgtgagcg gtcctgcccg atgaccccgc tcaacgtggt gctcgcggtg gccctcggcc
     3421 tgatcatctg gcgggtttcg atttctgtta ttcgcatgct gtcgacaccg ccacccgaga
     3481 tcgatcccgc tgatgtggtc gctgtcgacc aggactacaa gtgcacgatc tgcggcgcgg
     3541 agctgactat gcgggccatc aacgtccaag acgaggcgcc gcctcgccat tgtcgtgagg
     3601 acatgattcc ggtgtggcgg cccgcatgaa accgtctaat cacaagcgtg tagttatccc
     3661 gaggtttctc cacaccttgt tgagaactac acccttgtaa ctcgcattac gctcgccgcc
     3721 gtgcccgagt caaaacgccg gaagcccaag agcaagccac tgccgactca gtcggcggcc
     3781 gcggctgcca aggccaagcc accaagtccc acctggtatg tcgcggtgat ggccggcctg
     3841 atgacgatcg gcgtgctcct ggtcctgagc aggttcgtct tcgagctcga tcgcctggtt
     3901 ctgatcggtg gcctggcctg catcgccata ggcttcctga tgacgaccaa ctaccgctga
     3961 gttaggtgct cggcggcgtc gccgtcgtgg ttgtggtggt cggcggcggg gtgaacttgc
     4021 cgagcgtcac agtgaccaca tcacctggga acacctcgac accgtcatcg gggaattgag
     4081 cgaccaccct cccgtcctgt gaggcgtcca ccaccggttg ggtgctcgcc gagacattca
     4141 tttcgagacc aagatcggcg aggatgcctt ccgcctggcc ctgggtggag ccgagcaggt
     4201 tggggacttc aaccggcctc gggccttggg acacaaccag gaggatggtg tcaccggagc
     4261 gcacctcggc ccccgcttcg ggctcggttc tgatcaccag tccctcatcg acggtattgg
     4321 agaactcgtc gttgacagtc gagagtaacc ccagccgttg caaggcggtc acggcgtcac
     4381 gctcggtctc accctcgagc accgggagga tgtgggtttc ggcgccggtc gagaccgtga
     4441 tgctgatcgc ggcacctctt ccggcgagca cgccggagtt cgggtcctgt cgaatcaccg
     4501 tgccctcggc gaattcggcg ctcggctctt ccaccacctc cccgagaacg aagccgttct
     4561 gctcgatgat cctctcggcg gcttcctggg tttggccgat caggggtggc acctcgaact
     4621 cctcggctcc ggtagagtag aagacctcca cctcggagtc tggctcggcc tcggttccac
     4681 ccgccggatt ggtgcggatg accaggtcca cctccacgtc ctggctggct tcggcagtga
     4741 atttcacggt gaaccccgct gtgatgaggc gggcctcggc accttgtctg gtgagcccgg
     4801 cgacattggg gacttcgacc aggtcgttgc tgatgttgcc cccgccggcg agacggaaca
     4861 ggacgaacaa caagatcccg agggcgatca gaagggcgaa cgcagtgacg atgaatggca
     4921 tctgtgagga gggctcttcc tcgaccccgc gatagacctc gtcaggggga accgtggccg
     4981 gagggacctg ggcggtcatc actcgggtcg cgttgtctcc tgcggccgcc cctgcaacca
     5041 cagcgcccac caccgccggc tcgacgttct gtcctcggag aaccgcgacc aggtctgctc
     5101 gcatctcgtc tgcgctctga tagcggccgt tggggtcctt gcgcatggcc ttgcccacaa
     5161 ccgtgtcgag tgctggcggg acatcagcgt tgagggcaga aggtgcagaa gcttcggtgg
     5221 acacgtgctg gaaggcgacc gacatcgggc tgtccccggt gaagggcggt cgtccggtga
     5281 gcatctcgta aagcaccact ccaagggcgt agacgtcgct ccgtgcgtcg gctgcggctc
     5341 cctgggcctg ctccgggctg aaataggtgg cggtgccgat cactgccccg gttttggtca
     5401 attcggcgct gtcatcccaa gccctggcga tcccgaaatc ggtgaccttc accgtgccgt
     5461 ccttggcgag gaggatgttc ccaggcttga cgtcacggtg gaccaggccg gcctgatggg
     5521 cgattgagag cgcagctgcg acctcggagg cgatctcggt ggcccgacgg gcgagaagcg
     5581 ccccttccga cttgaggacg tcacgcaggg agcgtccgtc gaccagttcc atcacgatga
     5641 aatacgtgcc ctgggcctgt ccccagtcgt agatgccgac gatgtttggg tggctgaggt
     5701 tggctgccgc ctgcgcttca cgtcggaacc gcttcacgaa cgcctcgtca gccgaatact
     5761 gggcgtggag gaccttgatc gcgaccttgc gattgagcaa cgtgtcctgg ccttcgtaga
     5821 cgtcggccat cccgccccga gccaggtggg acgtgatgtg ataacgctcg gtcagttcac
     5881 ttggggtgtt catagaaggc gatggattgt aggaatgccc tgggcgcaac tcatggggtt
     5941 ccttgcaacc agagcgaaat caggtcggcg gcgatcggag ccgccacccc tccaccggtt
     6001 gcggattctc cgatgtcccc accgcccgcg atgaggaccg cgatggcaat cgtcgggtcg
     6061 tcgaccggtg cgaaccctat gaaccacgca tggggcccgt cacctgcgcc ttcggcggtt
     6121 ccggtcttgc cggcgaccct gatcccggga actgcggcat ttctcccggt gccttcggtc
     6181 accacccgtt ccatcatctg ggccaggacc gaggcggtgg caggagccat tgcccgtccc
     6241 agggagatgg gttgggaggt ttccagggtg ctcccgtcgg cgtcgaacac ccgattcacc
     6301 aggtacggat tgaatacttc gccctgattg gcgaccgctg cagcgaccat tgccatatgc
     6361 aacggagtag cgcgcacatc gcgttccccg atcccgcttt gtgccagggc ggccgggtcg
     6421 tcgatcagct cgtccacggg gtaggaggcc tgcgggaccg accagggaaa gtcgaggtca
     6481 gtgttgaatc ccaatgcctc ggctgtgatc ccgatgtcac gggcgcccaa tcgcactgag
     6541 agatcggcga agaccgtatt gcaggagcga acgaaggcaa ccagcagcgt caccgactcg
     6601 ccgtcgttgc agacctcccc gtcgaagttg gaaatggtct cggtggagcc ttcgagctgg
     6661 tactcgacag ggtcgggaaa ttcggtcgaa ggatctgcct cgcctgtgtc caacgccgtc
     6721 gcggtaacga cggtcttgaa ggtcgaaccc ggcgcgaata gctctttggt cgatcggtcg
     6781 ctgagcgggc tcaccgggtc ggcgagcagc tcgtcccaca ccgccgccgc gtcctccccg
     6841 aggagcgatt cgggatcgaa tgaaggcgag gagacagcgg cgagcaccgc gcccgtcttc
     6901 gggtcgatcg caacgacggc gccggtctgt ccgcccaacg cctcgtaggc ggccatctgc
     6961 agtgggccgt tgacggtcat ctcgatgctg tgaggacgca ggtcgcgccc gagaatcacc
     7021 gaaatcaggt cgctgatcgt caggtcgcgt ttcgatctga gcacaggggc gtaggcctcc
     7081 tccagtccgg agtcaccgac gatgaacgag ttgtagccga ccacatgggc gaaagcctca
     7141 ccttccgggt aggtgcgcac gaaggagcgt gggtcatcag ggtcagccac cgattcggcg
     7201 accacagttc cgtcgatggt cacaatcagg ccccgttcct tgccccgctc ggagagggcg
     7261 ggcctggcgt tgcgaggatc ggcctttaac gcgtcggcgt gaatcacctg gatccaggtg
     7321 actgccagga gaaggacaaa gaaggaggta aagaccccga tcgagatgcg acgaaccggg
     7381 ccgttcatgt tgcttcctca tggctgatcc ggagcaagag ggcgatcagg atcagattgc
     7441 cgaccagggc cgatccccca tatgacatga agggaagcgt gatcccggtc agggggacca
     7501 ccctgaccac cccaccgatg atcagaaagg tctgaacggc gaaaacgaac gtgagaccgg
     7561 ccgccagcag cttgcggaac atgtcccggc tccggaatga gatcccgagc ccgaccgcga
     7621 tcaccaacga gaacagtgcg agcactgaca ccgtccctgc gaatcccaat tcctccccga
     7681 ccgcggcgaa gatgaagtcg gtcgaggcat ttgggatcag gtcggggcgg cccaggccgg
     7741 gcccagcccc agagaggctc cccgtcccca ttccgaaaag gccctgtgcg atctggtacc
     7801 cctcgtcgtc gaagtgctcg aaaggcgcca gccaggcgac gacccgccgc tgtacgtgtt
     7861 cgaacatgaa gtaggaggcg acaccggcag ccgccgccat gaacccacct gtcaccagat
     7921 agccggtgtt cccggttgcg gcgtagagga ggagcacgaa cccggcaaag agcaacaggg
     7981 aggcgccaag gtcgcgctgg gataccagga tcaggatgct caccatccag gtgatcacca
     8041 gcgggatcag ctgccgcggc tcggggaagc gaatcgggcc tagatgccgc cgcgcctcac
     8101 gcagcgcctt ctgatggtcg gccaaatagg ccgccacgta gatgacaaag cccagtttgg
     8161 ccagctcggc gggttggaac tgaagggtcg tgaattcgag gtcgaggact atccagagcc
     8221 gggatccgtt gacaacgagc cccttgagcg gaaagcccca atcggtcggc aggttgggta
     8281 gggccagcag tatgaacgag acgagaagag tgatgttgcg gtaacggcga aggatggttg
     8341 tcgagctccg tgcgaacaga gcgaggacag cgatggcggc ggcggaggcg atcaacatcc
     8401 accagcgctg caatcccgct tttaccggat ccagcctgta gatctcgatg aacccgatcg
     8461 cagtgagcgc ggtgatcggg gcgatgagaa ggggtgtggc tcgcggcgcc cacgtccgca
     8521 ccgccagatg cagaccgcca aaggcgatca ggaaaaccag gaaggtaagt gcggtctggg
     8581 cgtcgacact ctgttcgcgg gcaaagttca ccagcgtgat gccaaaagcg gcgagcaccg
     8641 acgccgcgac cacggttgcg ccttcggcgt tacgtgtcat tcgctccggg gaggacatcc
     8701 acgatgatga cgctgatgtt gtcgtggccg cccgcttggt tggactgctc gaccagcgcc
     8761 caaaccgttt cgtctgcaga ctcagtgccc aggttctcgg cgatctcgtc atcggtgagc
     8821 atcagggtca ggccatcgga gcaaagcagg agtcggtcgc cgttctcgag cggcaactcg
     8881 atctcgtcga cattcacgaa tcgagtgagc ccgagacagc gggtgagcat gtgactgtgg
     8941 ggatggtcgg ctgcttcctc cggcgagagg gtcccagcag cgatgtattg ggcgacaacc
     9001 gtgtgatcgt ccgtcaactg gcgcaattct ccggctcggt agatgtaggc gcggctatca
     9061 ccgatgtggc cgaagtgtgc agtcccgttg gcggtcaact ccaccagggt gagcgtggtt
     9121 gccatcccct cgagatcagg ttgccgggcc acctgctctc gtatggcgcg attggccgca
     9181 gccactcgat ctgccgggcc gagatcggcg gacgctgccg tgttcaccgc gagacgagag
     9241 gcgacttcgc cggcgatgtg cccacccatg ccatcggcca ccatgaggac agcaggaccg
     9301 gtcgactccc ctgaggtctc ggggaagatg gtgtcctcgt tgttcgtcct cgccatgccc
     9361 tgatgggtgg cggcggccca
//
LOCUS       DBNHXU010000025         4450 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3579055, whole genome shotgun sequence.
ACCESSION   DBNHXU010000025 DBNHXU010000000
VERSION     DBNHXU010000025.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4450)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4450
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3579055"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..661)
                     /locus_tag="AC161X_00730"
     CDS             complement(104..661)
                     /locus_tag="AC161X_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014741922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HhH-GPD-type base excision DNA repair protein"
                     /protein_id="HIR4988101.1"
                     /translation="MDRPDTLPFTESDEANRLLATDGLALLIGMLLDQQFPMERAFYG
                     PQLLKDRLGEDLDAARLAEWDPENLESIFKGPPAIHRYPGSMAGRTQEMCRVIVAEYE
                     GRAENLWETADDGTELLKRLQALPGFGVAKSRIFVGVLGKRLGVRPDGWEEKAADWAS
                     IADVAAFEDVATLREKKRAMKARKE"
     gene            875..2086
                     /locus_tag="AC161X_00735"
     CDS             875..2086
                     /locus_tag="AC161X_00735"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009711847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HIR4988102.1"
                     /translation="MSEDVYIADGCRTPMGKLGGQLSHVRPDDLAAHAISRLLARNPN
                     VDPMAIEDIQWGAANQAGEDNRNVARMAGLLAGLPIEIPGATVNRLCGSGMQAVVSAS
                     HALAGGWGDLMVAGGSESMSRAPYVITKSDRAFPTGFPEAADTVIGWRLVNPRMTELY
                     PPITLGMTAEMVAEKYHVTREDQDAFALRSHQLALAAVAAGRFGQEIEPIPAPTDHRR
                     REMATVTADEGPRADTSLERLAALAPVFKAGGTVTAGNSSPMNDGAAALLLATQAGLE
                     SHGLKPLARIVSSAAAGVHPDVMGIGPVPASEKALARAGIGVGDLDLIEVNEAFASQA
                     LASMRLLELDEEKVNVNGGAIAIGHPLGASGARILVTLVHELARRQGRYGLATMCIGV
                     GQGIAVVVERV"
     gene            2079..3260
                     /locus_tag="AC161X_00740"
     CDS             2079..3260
                     /locus_tag="AC161X_00740"
                     /inference="COORDINATES: protein motif:HMM:NF014564.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA transferase family protein"
                     /protein_id="HIR4988103.1"
                     /translation="MSEGPLHGTLVVEFGNLIAAPYAAMLLGDLGARVIKVEPPDGDL
                     GRQFGPFQNGESAFFMAVNRGKESFALDTKDWLSKRILDNLVKKADVVISNLRLGAME
                     RLGLGEVRSRDLNPKLIYAVISAFGAEGPYARRAGIDVVFQGESGMISITGDPKDGPH
                     KTATSIGDYLAGTNLALAVSAALVDKRSRGCRIDVSLRDGVMAVQSGWNALAFTSGEQ
                     PTRTGTASPFIAPNQVFEASDGPFTLAIVSERHFELLADALERPDLVKDYPTNESRMS
                     NRAQLARRLSNVFKTEPVEYWVDLLEQVGLPAGRVLTLAEAFADPQARHNEMTVEMEH
                     PIAGAIKVTGSPIRVDGSPARASTPPATLGQHTASILHELGMDKSTIEQMVEAGTAVV
                     S"
     gene            3265..3633
                     /locus_tag="AC161X_00745"
     CDS             3265..3633
                     /locus_tag="AC161X_00745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988104.1"
                     /translation="MIHWAPEVGDDWGEVLTALTGAFEASRAAAQARESIVYVVHNDD
                     LLGRRGAGKAMLATGLLSAARTAALEGVKDGWTANVIAVSNEVDREQVAHWAGQLIES
                     ADVTGEVLRIGPDHIGKALP"
     gene            3648..4328
                     /locus_tag="AC161X_00750"
     CDS             3648..4328
                     /locus_tag="AC161X_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005256876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4988105.1"
                     /translation="MTGAARGIGRVIAEKVASTGPLTVIGADLAPELALAEQITPVQV
                     DLTSAEGPGIVRKAVQDSGLPLLGLVNNAGITRDSRLINMTDGDFRSVLDVNLGAAFR
                     LCAELAPLMSDGASIVNIASRAYLGNFGQFNYSMSKGGVVGLTRALALSLAPAIRVNA
                     VAPGLISSEMSMGIPEDVRDRMVAAIPLERMGKPEEVANLVWFLLTPLSSYITGEVIV
                     VGGGRSLS"
BASE COUNT          814 a         1398 c         1435 g          803 t
ORIGIN      
        1 gtcagcgagt tgccccgaaa gctgcatctg tgattccata gatgaggtcc ctgtcccttt
       61 tgggcactat cggcattcgg ggccgaacag ttgagcctgt tgatcactct tttcgagcct
      121 tcatggctct ttttttctcc cgaagagtgg ccacatcctc gaaggccgcc acgtccgcga
      181 tgctggccca atcggccgcc ttctcctccc agccgtcggg tcgcactccc aaccttttac
      241 cgaggacgcc aacgaagatc cgcgacttgg cgaccccgaa tccaggcagc gcctggagcc
      301 gcttcagaag ctccgtcccg tcgtcggccg tctcccacaa attctcagcg cgaccttcgt
      361 attcggccac gatcacccga cacatctcct gggttcggcc cgccatcgaa ccggggtagc
      421 gatgaatcgc tggtgggcct ttgaaaatgc tctccaaatt ctcgggatcc cattcggcga
      481 ggcgagcagc atcgagatct tccccgagac ggtccttgag gagctgcggg ccgtaaaagg
      541 cccgctccat gggaaattgc tggtcgagga gcatgccgat gagcagagca agcccgtcgg
      601 tggccagcaa gcggttggcc tcgtcggact cggtgaaagg gagagtgtca ggtcggtcca
      661 tgggaccaac cgtagttatc aggacaggat gcgaatcagg acgtagagca ccagcaggcc
      721 ggccggggtg taggcggcga ttgagccgtg atgtcggttc tgtccgttca tactcatatc
      781 tgacgtcacc gtatggtgaa aaggtttcat cggatatggg ggttgtcccc cattcgaccc
      841 ttccagtttc ggcaagaatg agaatcgact gaagatgagc gaagacgtct acatagccga
      901 cgggtgtcgg acccccatgg gcaagctcgg aggccaactc tcccatgtca ggcctgacga
      961 tctcgccgca cacgcgataa gccgcctcct ggctcgcaat cccaatgtcg acccgatggc
     1021 catcgaagac atccagtggg gcgccgccaa tcaggctggt gaggacaacc gcaatgtggc
     1081 ccggatggcc gggctcctcg ccgggctccc catcgaaatc ccgggggcca cggtgaaccg
     1141 cctttgtggg tccggcatgc aggctgtcgt ctcggcttca catgcactgg ccggcggctg
     1201 gggagacttg atggtcgccg gcgggtccga gtcgatgagt cgcgctccat acgtgatcac
     1261 caagagcgat cgagcctttc cgacagggtt tcccgaagca gcagacacgg tcatcggttg
     1321 gcgtttggtc aatccaagga tgacggagct ctatccgccg atcacgctgg ggatgacagc
     1381 ggagatggtt gccgagaaat accacgtcac cagggaggat caggacgcct ttgccctccg
     1441 cagtcaccaa ctcgccctgg ccgcggtcgc ggctgggcgg ttcggtcagg agatcgagcc
     1501 gataccggct cccaccgacc accggcggcg cgaaatggcg actgtcactg cagacgaggg
     1561 ccctcgagcc gacacctcac tggagcgtct ggctgccctt gccccggtgt tcaaagcggg
     1621 cgggactgtc accgccggca actcatcccc gatgaacgat ggcgcggcgg ccctgcttct
     1681 ggctacccaa gctggtctgg agtctcacgg cctcaagcca ttggcccgga tcgtgtcttc
     1741 ggcggcggcc ggggtccatc ccgacgtcat ggggatcggc ccggtgccgg cctcggagaa
     1801 ggcgctagcc cgagccggga tcggggtagg cgacctcgac ctcatcgagg tcaacgaggc
     1861 cttcgcctcc caggcgctgg ccagcatgcg gctgctcgag ctcgatgagg agaaggtcaa
     1921 cgtgaacggc ggtgctatcg caatcgggca cccgctgggc gcctcggggg ctcgcatcct
     1981 cgtcaccctc gtccacgagc tggcccggcg tcagggccgt tacggactcg ccaccatgtg
     2041 cattggcgtc gggcaaggga tcgccgtggt cgtcgagcgt gtctgaaggc ccgctccacg
     2101 gcaccctcgt agtcgagttc ggaaatctga tcgcggctcc gtatgccgcc atgcttctcg
     2161 gcgacctggg cgccagggtc atcaaggtgg agcctcccga cggagatctg ggacgtcagt
     2221 tcggtccttt ccaaaacggc gaatccgcct tcttcatggc cgtcaatcgg ggcaaagagt
     2281 cgtttgccct cgacaccaag gactggctct cgaagcggat cctggacaac ctcgtcaaga
     2341 aggccgacgt ggtcatcagc aatctccggc tcggtgccat ggagcgtctc gggttggggg
     2401 aggtgcggtc ccgggacctg aacccgaagc tgatttatgc cgtgatctcc gccttcggcg
     2461 ccgagggccc atacgccaga cgagccggga tcgatgtcgt cttccagggt gagtcaggga
     2521 tgatctcgat cacgggcgac cccaaggacg ggccgcacaa gacggcgacc tcgatcgggg
     2581 actatctggc cgggaccaac ctggcattgg ctgtatccgc agctctggtc gacaagcgat
     2641 cgaggggatg ccggatcgat gtctcgctcc gggacggtgt catggcggtc cagtcagggt
     2701 ggaacgcttt ggcgttcact tcaggagaac agccgacccg aaccggaacc gccagcccgt
     2761 tcatcgctcc gaaccaggtg tttgaggcgt cagacggccc ctttaccttg gcgatcgtct
     2821 ccgagcgcca cttcgagctg ctcgccgacg ctttggagcg gccggacctg gtcaaggact
     2881 atccgaccaa cgagtcacgc atgtcgaatc gggcccaact ggcgcggaga ctcagcaacg
     2941 tgttcaagac agaacccgtc gaatactggg tcgacctctt ggaacaggtg ggcctacccg
     3001 ctgggagggt gctgaccttg gccgaagcct tcgccgatcc ccaggcgcgt cacaacgaga
     3061 tgacagtcga aatggagcat ccaatcgccg gagcgatcaa ggtcaccggg tccccgatcc
     3121 gcgtcgacgg ctcaccggcg agggcctcca ccccgccggc gactttgggt cagcacaccg
     3181 cgtcgatcct tcacgaactg gggatggaca aaagcaccat cgagcaaatg gtggaggccg
     3241 ggaccgccgt cgtatcgtga cgcggtgatc cactgggcac cggaggtggg cgacgactgg
     3301 ggcgaggttc tcaccgcact caccggagca ttcgaagcca gccgagccgc ggcccaagcc
     3361 agggagtcga tcgtctacgt cgtgcacaac gacgacctgc tcggacgccg gggcgccggg
     3421 aaggcaatgc tggccaccgg cctgctatcc gcggcaagaa ctgcagcgtt ggagggtgtc
     3481 aaggacggtt ggacggccaa cgtgatcgcc gtttccaacg aagtcgaccg ggagcaagtt
     3541 gctcactggg caggtcagct gatcgagtca gccgatgtca ccggagaagt cctgaggatc
     3601 ggacccgacc acatcggcaa ggccctgccg tgaatggtgt catcgtcgtg acaggtgccg
     3661 cacgtggcat cgggcgagtt atcgccgaga aggtggcgag caccgggcca ctcaccgtga
     3721 tcggcgccga cctcgcccct gagctcgccc tggccgaaca gatcaccccg gtccaagtcg
     3781 acctgacctc ggccgaaggc ccggggattg ttcggaaggc agtccaggac tcagggctcc
     3841 ctttgctcgg actggtgaac aacgccggga tcaccaggga ctctcggctg atcaatatga
     3901 ccgacggtga cttccgctcg gtgctcgatg tgaatctggg cgccgccttc cgactgtgtg
     3961 ccgagctggc cccattaatg agcgacggtg cctcgatcgt caacattgca tcgcgggcct
     4021 acctggggaa cttcggccag ttcaactact cgatgtcgaa gggtggggtg gtgggactaa
     4081 cccgtgctct ggccctgtcg ctggctccgg cgatccgggt caatgcagtg gcgccggggc
     4141 tgatctcttc ggagatgagc atgggcatcc ccgaggatgt gagagaccgg atggtggcgg
     4201 cgatcccctt ggagaggatg ggcaagccgg aggaggtggc caatctggtg tggttcctgc
     4261 tcaccccgct ctcgagctac atcacgggag aggtgatcgt ggtcggcggg ggacggagtc
     4321 tctcgtaggt tggcttggca gggcgccccc ctccccgcct ccggcggtac tccccccaac
     4381 gctcacttcg ttcactgggg ggagaaactc agtagtagta cctgctgacc gtctcggcga
     4441 ccatcgccgg
//
LOCUS       DBNHXU010000026         4257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_404693, whole genome shotgun sequence.
ACCESSION   DBNHXU010000026 DBNHXU010000000
VERSION     DBNHXU010000026.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4257
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_404693"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..862
                     /locus_tag="AC161X_00755"
     CDS             <1..862
                     /locus_tag="AC161X_00755"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4988106.1"
                     /translation="SSKSSLWVTDRNSSKMFEISFDGTTPPSTTSTSTTQQTTTTTQA
                     TTSTTSGTTTTTQGNPTTTTTQATTTTTSGGTTTTTTMGTTTTTIPGSTTTTTQPGGG
                     GGGGTNPFIDDDGSVFENSIEWLAAAGITQGCNPPANNRFCPNDRVTRGQMATFLVRA
                     LNYPGTSHDWFIDDESSVFENSINRLREAGVTQGCNPPDNSRYCPNALVTRGQMAAFL
                     DRAFGYSAGGSIDYFTDDNGHVFENAINRLRTARVTLGCNPPSNNRYCPNDFVTRGQM
                     AAFLKRAMGG"
     gene            898..1875
                     /locus_tag="AC161X_00760"
     CDS             898..1875
                     /locus_tag="AC161X_00760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR4988107.1"
                     /translation="MVSSESTEVASTLGVVIPTIGDRPELKRLLLTILAQTRPVQAIQ
                     VVVDADDTTLVDAILAELQGDLGRTVVEVQRTGVMRTEGDYLVDTGYGYTVNRGLERL
                     DTDLVSFLDDDDEIRPSHFAQLEAALDPSAGRSASYSRVLVVNPDGAERHHPEGTMPA
                     GKIPVGVLIDAHPVLLPATLMHRSVLDTVQSLDQTLDREADTDMIVRLGLATEFAAVD
                     DPTYVYYRVSRKAIVNERVLSERSRMLHKHQSHLTRREKLHLWDPIGRSALRAGFTEI
                     GRDAGEQVISALWNHAPGFLVGWYVFLRGRQTPEFIKRAVKRLSGGPGQ"
     gene            1872..2825
                     /locus_tag="AC161X_00765"
     CDS             1872..2825
                     /locus_tag="AC161X_00765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR4988108.1"
                     /translation="MTETSDLARQLLELQEEFHWESKSAAYELVASWERERSGSVFHR
                     LIGKPSGPEVVVKVSADWASEDALRTFEAMIDLADAIEAADIAGAAAIRPLAWSEAPP
                     QLVMPFVDGADVVSILRQPDHPAWREDVATWMRSAGGMLSAFHARNVAAGSAEALAEV
                     RALAERFRVRRNFDGVDWQGRTTGSFGDFGPGNLHAGADGVLYLLDPPLVAVSSPVHR
                     DLGNFTFELRRQLDGRGYTASPPVTGHFDRLCDAFLQGYSDAWGSTLDETDLALIALF
                     ELRRAAGMARKRFPGRLGDALWFARSALARRREVIATPDHS"
     gene            complement(2816..>4257)
                     /locus_tag="AC161X_00770"
     CDS             complement(2816..>4257)
                     /locus_tag="AC161X_00770"
                     /inference="COORDINATES: protein motif:HMM:NF049778.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988109.1"
                     /translation="DPNKSITLTASGNVLVLGELVMKPASADIEHALIFQGVDNSAFV
                     GGGMDPVPTDVGLWVMGDGRLDLEGEEKPAWGYEFDPSWLGDEVMAAPNTQGDYYVFE
                     EMTSTPPPNELGYPTELMNLTRNVRIEGTPEGFTHVFLRSTQPQTIRYVALRYMAPHF
                     GDSDRTGRYGLHFHMAGEGSRGSIVEGVVVRDAGNHAFVPHASHGITFRDTIAYNVLN
                     EAYWWDPAPEEEDTTNDTNDLIWDRAVVAGVDLGVEGNKFRLAGFFLGNGSNMTISNS
                     VAVGVRGEPDSESSGFIWPEGAGAVWLFENNVAHNNLSNGVFVWQNNAEVHPVDGFTG
                     YYNGKAGIEHGAYENSYQYHDLALPGNGVAIISHALGGESDQGEVDTQTWTNITSGGG
                     TLIIDKHATQPEVPVRFMDCDFGLVVVADEEGGVPGKYDFVNCDLQPDEFDLESAYGS
                     SVFRVQNADGTTYQLLGNGTETEIAPFYE"
BASE COUNT          827 a         1398 c         1260 g          772 t
ORIGIN      
        1 ctccagcaag tcgagcctct gggtgaccga tcgcaacagc tcgaagatgt tcgagatctc
       61 cttcgacggg acgacgcccc cgtcaacgac ctccacgagc accacccaac agaccacaac
      121 tacgacccag gcgacaacgt ctacgacctc tggtaccaca acgaccacac agggcaaccc
      181 gaccaccacc acgactcagg ccacaaccac taccacttcc ggcgggacga ccaccaccac
      241 aaccatgggg acaaccacca caacgatccc cggttcaacc actacaacca cgcagccggg
      301 cggcggcggt ggaggaggca ccaacccctt catcgatgac gacggctcgg tattcgagaa
      361 ctccattgaa tggctggcgg ccgcggggat cacccagggc tgcaatccgc ccgccaacaa
      421 caggttctgc cccaatgaca gggtcactcg cggacagatg gcgaccttcc tggtccgagc
      481 cctcaactat ccgggcacta gccacgactg gttcatcgat gacgagagca gcgtgttcga
      541 gaactcaatc aatcgtctcc gggaagccgg ggtcacccaa gggtgtaacc ctcccgacaa
      601 cagccggtac tgcccgaatg ccttggtcac ccgaggtcag atggccgcgt tcctggatcg
      661 cgcctttgga tattcggctg gcggatccat cgactacttc accgatgaca acgggcacgt
      721 cttcgaaaac gcgatcaaca ggcttcgcac cgctcgggtg acccttgggt gtaacccacc
      781 cagcaacaac cgatactgtc ccaacgactt cgtcaccaga ggacagatgg cagcctttct
      841 caaacgggcc atgggaggct gaccgacgct gagtggcggt cggctcagga ggcaactttg
      901 gtcagttcgg agagcacaga ggtcgcctcg actctcggtg tcgtgattcc gactatcggg
      961 gatcggccgg agttgaagcg cctgctcctc acaattctcg ctcagacccg cccggtccaa
     1021 gccatccagg tggtcgtcga cgccgacgat acgactctgg tcgacgccat cctcgccgaa
     1081 ctccagggtg atctggggag aaccgtcgtg gaagttcagc gcacgggggt gatgcggacc
     1141 gagggcgact atctggtcga caccgggtac ggatacacgg tcaatcgcgg gctcgagcgt
     1201 ctcgacacgg atctggtctc cttcctcgac gacgacgacg agatacggcc ctctcatttc
     1261 gcacagctcg aggcggctct ggatccttca gctgggagaa gcgcctccta ctcaagagtg
     1321 ttggtcgtga atcctgacgg tgccgagcgt caccacccgg agggcacgat gcctgccgga
     1381 aagatccccg tcggagtcct gatcgacgcc catccggtgc tgcttccggc gaccctcatg
     1441 caccgatccg tcctcgatac ggtgcaatcc ttggaccaga ccttggaccg ggaggcagac
     1501 accgacatga tcgtgcgcct cggcctggcg acggagttcg ccgcagtcga cgaccccacg
     1561 tatgtctatt accgggtctc gcgcaaggca atcgtcaacg aacgcgtgct atccgagcgg
     1621 tcgcgaatgc tccacaaaca ccagagccac ctgaccagac gcgagaagct tcacctctgg
     1681 gatccgatcg ggagatcagc gttgcgagcg ggattcaccg aaatcggtcg cgacgccggc
     1741 gaacaggtga tctccgccct ctggaaccac gctcccgggt ttctcgtcgg gtggtacgtc
     1801 ttcctgcggg ggcgccagac cccggagttc atcaagagag cggtcaaacg cctcagcggc
     1861 ggacccggtc aatgaccgag acgtcggacc tcgcccgcca attgctcgaa ctccaggagg
     1921 agttccactg ggagtccaag tccgccgcat acgagctggt ggcaagctgg gagcgcgaac
     1981 gttcgggatc tgttttccat cgcctgatcg ggaagccatc aggacccgag gtggtggtca
     2041 aggtgtcggc cgattgggct tccgaagacg ctctacgcac cttcgaagcg atgatcgacc
     2101 tggcggatgc catcgaggcg gccgacatcg ccggagccgc cgccatccgc ccgctggcat
     2161 ggtctgaagc tccaccgcag ctggtcatgc ccttcgtcga tggagccgac gtggtttcga
     2221 tcctccgcca gcctgatcac ccggcctggc gagaggatgt cgcgacctgg atgagatcag
     2281 ccggaggaat gctgtccgct ttccacgccc ggaatgtcgc cgccggatca gccgaggcat
     2341 tggccgaggt cagagcactc gccgagcggt ttcgggtccg ccgaaacttc gacggagtgg
     2401 actggcaggg ccggacaacc ggttcatttg gtgatttcgg acccggaaac ctccatgccg
     2461 gtgccgatgg tgtcctctat ctgctcgatc ccccgctggt cgcagtttcg agcccggtgc
     2521 accgcgacct ggggaacttc accttcgaat tgagacgcca actcgacgga cggggatata
     2581 cggcaagccc acccgtgacc ggtcatttcg accggctctg cgacgctttc ctccagggct
     2641 attccgacgc ctggggctcg acactcgatg aaaccgacct ggcactgatc gcgctgttcg
     2701 aactgcgacg cgccgccggc atggctcgga agcggtttcc ggggcgtttg ggcgatgccc
     2761 tctggttcgc ccgctccgca ctggcgagac gacgcgaggt gatcgcaacc ccagatcatt
     2821 cgtaaaaggg ggcgatctcc gtctcggtgc cgttacccaa tagctgatag gtggtcccgt
     2881 cggcattctg cacccggaaa accgacgatc cataggccga ttccagatcg aactcgtcgg
     2941 gctgcagatc acagttcacg aagtcgtact tgcccggaac accgccctcc tcgtcggcca
     3001 ccacgaccag gccaaagtcg cagtccatga atctcaccgg cacctcgggc tgggtggcgt
     3061 gcttgtcgat gatgagggtg ccaccaccac tggtgatgtt ggtccaggtc tgagtgtcca
     3121 cttcaccctg atcgctttcg cctccgaggg catgggaaat gatcgccacc ccgttacccg
     3181 gcagagcgag gtcgtggtac tgatatgagt tctcataggc gccgtgctcg atgccggcct
     3241 tcccgttgta atagccggtg aacccgtcga ccggatggac ttcggcgttg ttctgccaga
     3301 cgaagacacc attcgacagg ttgttgtggg cgacgttgtt ctcgaatagc cagactgcgc
     3361 ccgccccctc cggccagatg aaccccgagc tttccgagtc gggctcgcca cgcactccca
     3421 ccgcaaccga attgctgatc gtcatgttgc tgccgttgcc gagaaagaat ccggcgagcc
     3481 gaaacttgtt gccctcaacg ccgaggtcga cccccgccac caccgcccga tcccagatga
     3541 ggtcgttggt gtcgttggtg gtgtcctcct cctcgggggc agggtcccac cagtacgcct
     3601 cgtttaggac gttgtaggca atggtgtcgc ggaaggtgat gccgtgcgag gcgtgaggga
     3661 cgaaggcgtg gttgccggca tcccggacca ccaccccttc cacgattgag cctcgactcc
     3721 cctcgcccgc catgtggaaa tgaagcccgt agcgcccggt ccggtcgctg tcgccaaagt
     3781 gaggggccat gtaacgaagc gcgacgtagc gaattgtttg cggttgggtg gagcggagga
     3841 agacatgggt gaaaccctcc ggggttccct cgatccggac gttgcgggtc aggttcatca
     3901 actcggtcgg gtaacccagc tcgttcggag gcggggtcga cgtcatttcc tcgaacacgt
     3961 agtaatcgcc ttgagtgttg ggggcagcca tcacctcatc ccccagccac gacgggtcga
     4021 attcgtaacc ccaggcaggt ttctcctccc cctcgagatc caaccgaccg tctcccatca
     4081 cccacaggcc gacgtcggta ggtaccggat ccatcccccc accgacgaaa gccgagttgt
     4141 cgacgccttg gaatatcagg gcgtgctcga tgtcagccga tgccggcttc atcaccagct
     4201 cccccagcac cagcacattc ccggacgcgg tgagggtgat cgacttgttg gggtcga
//
LOCUS       DBNHXU010000027         5608 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_801207, whole genome shotgun sequence.
ACCESSION   DBNHXU010000027 DBNHXU010000000
VERSION     DBNHXU010000027.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5608)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5608)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5608
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_801207"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..548
                     /gene="udk"
                     /locus_tag="AC161X_00775"
     CDS             <1..548
                     /gene="udk"
                     /locus_tag="AC161X_00775"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF004018.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIR4988110.1"
                     /translation="SVLDRLDGQVALVHHDAYYRHAPELSYEERTKVNYDHPSSLETE
                     LLVEHLMALRSGVAIERPVYDFAVHLRSDVTVRVEPAPVVVVEGILVLAEPELRSELD
                     LKIFVDTDSDIRLARRLQRDIEERGRTVDSVVSQYFETVRPMHIEYVETSKRYADLII
                     PEGYNPRAVATVVELIRSRLG"
     gene            577..1389
                     /gene="proC"
                     /locus_tag="AC161X_00780"
     CDS             577..1389
                     /gene="proC"
                     /locus_tag="AC161X_00780"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012085059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004735 - pyrroline-5-carboxylate
                     reductase activity [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HIR4988111.1"
                     /translation="MSGRTSVAIIGVGAMGEALMSGLFRAGWEPADVSLCVRRPDRAQ
                     SLTEISGALVSSDPIETIRDRDVVVVAVKPRDVPSLLDHLSGHVGPEQMVLSLAAGVT
                     TGTYESRLGAVPVVRAMPNTPALVGEGVTGIAGGANAGSDHLDLAGEILGAVGSIRIM
                     DESLLDAVTAVSGTGPAYVFLLAEALTEAAVREGLPRDIAENFVHQTIRGAGHLLTST
                     GKTPAELRSQVTSPGGTTAAAMHVLEERGFRALVEDAVAAAARRSTELGGKS"
     gene            1443..3620
                     /locus_tag="AC161X_00785"
     CDS             1443..3620
                     /locus_tag="AC161X_00785"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR4988112.1"
                     /translation="MALTQERQDEATTSKRPSVSYVIEGLGVVGALATLALGLLGTIP
                     EEPEVEIGRAVFGNIPAAVQFVFYVTVSVFIWVSAHLLARRAASWQRGRPESRVGLWG
                     RRIKDLDAGLRMKTLMRDPRAGVMHSLIYYGFIVLFLGTVTLEIDHLLPANLKFLQGP
                     VYQGYSAILDLAALAYLGGLALAIANRYLLRSPRLRTKTKPEDALILGLLVLLGVSGL
                     ITEAARISLGGRPDFEVWSFVGYPLSALIPAASASGLHQAFWIGHVVGFVAFLVILPV
                     TKLRHMVTAPANLFLSAGPRPKGAMREMPNLLEAEDIDTIGASLAADLTWKQIFDTDA
                     CTICGRCTSVCPANITGKPLDPREIVLKTGEVAAAAAGVSPAGSSIFLLEGEPSLFDR
                     VRPEEVFACTTCRACDEICPVNIEIVDRILDMRRYLTLMDSDFPSELGKAFVAMENQS
                     NPWGMSQQNRAAWTEELDFEVPIFGENGKTTAEYLWFVGCAGSFDDRNTAVSVALARL
                     LNKAGIDFAILGRAELCNGDPARRAGNEFVFQQLALQNIANFDEIGVKKVITQCAHCF
                     NTISNEYPQFGGHYEVVHHSQLLAELLSQGRITVPLKANGQPKKIAYHDPCYLGRHND
                     VYLAPRNVIGASGDIELVEMPRHGTRALCCGAGGARFWMEEQTGKKVNIERAEEALAT
                     DADEIAVACPFCYVMLDDGVKELGRDDVPVRDLAMILADGVGP"
     gene            3662..4192
                     /locus_tag="AC161X_00790"
     CDS             3662..4192
                     /locus_tag="AC161X_00790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988113.1"
                     /translation="MLALLAACTPEAGGPSGTDSQSTSTTTPTTTSTTTITGVAVEEF
                     RTCLAEHDVDIEEVPLDATGRPRLDLVIVSVDFGDPEVADALAVCSASLNTGALDLGD
                     DEELRREVNDELLEFSKCMRANGVEDFPDPVPGFAGVGAPFAVAEIPYGDPELATASA
                     ACRASVLGGLPGLDGS"
     gene            4212..4496
                     /gene="clpS"
                     /locus_tag="AC161X_00795"
     CDS             4212..4496
                     /gene="clpS"
                     /locus_tag="AC161X_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012229018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HIR4988114.1"
                     /translation="MTTRPSPVITPETGESTDVDLPWQVIVWNDPVNTMAYVVYVFRK
                     LFGYDVEKATKLMLEVHFEGRSRVSDGSREKAEIDCFRLHGHGLWATMEQ"
     gene            4493..4954
                     /locus_tag="AC161X_00800"
     CDS             4493..4954
                     /locus_tag="AC161X_00800"
                     /inference="COORDINATES: protein motif:HMM:NF020989.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2017 family protein"
                     /protein_id="HIR4988115.1"
                     /translation="MSRGRFRYKSGKIVVELPKEEREFLSDVLELLSSIPSSGDDPAT
                     RRLQVPVYLDDPEANSEWWLFMGEELSIARSADRDVYQRVMDSEKRITLTTEEADAFL
                     RVLNEGRLALGARFGLEVEADHDQLPEEQRQVLDYLGWTLEEMTVALSAAL"
     gene            5004..>5608
                     /locus_tag="AC161X_00805"
     CDS             5004..>5608
                     /locus_tag="AC161X_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988116.1"
                     /translation="MPEPATDIQHAGLARRHLLPSDPIAGLRSGVGALITIYLNRPGP
                     GGMAALLTDLLKPVRDEAERIGGFVRKSVRADATRIHELANQLEGDVAPAFAIFAADV
                     DGLFVLQPLAYSVPNVSTLGPRPYLRPLRAAPRPLRAGIIVADRASARIFIGSDDLVE
                     ELAAYSVDIGKPNYGGFGGYEEHNARAHAGEETNRMWKEAGA"
BASE COUNT          961 a         1785 c         1794 g         1068 t
ORIGIN      
        1 aatcggtcct cgatcgtctc gacggacagg tggccctggt ccaccatgat gcctactacc
       61 gacacgctcc tgagctgagc tacgaggagc ggaccaaggt caactacgac cacccgtcct
      121 cgttggagac cgagttgttg gtcgaacacc tcatggcctt gcggtcaggc gtcgccattg
      181 agcgccctgt atatgacttc gctgttcacc tccgctcgga cgtgacggtc cgggtcgaac
      241 ctgcgcccgt tgtcgtggtg gaggggatcc tggtgcttgc cgaaccggag cttcggtcag
      301 agctcgacct gaagatcttc gttgacaccg attccgacat ccgtctcgcc cgtcgtctgc
      361 agagggacat cgaggaacgg gggcggacag tggactcggt ggttagccag tacttcgaga
      421 ccgtgcgccc catgcacatc gaatatgtgg aaacttcgaa gcgctacgcc gacctgatca
      481 tccccgaggg ctacaacccg agggcagtgg cgacggtggt cgagctgatc agatcccgcc
      541 tcggctgacg agctggcctc tccatatact cgcccgatgt ccgggcgcac atccgtagca
      601 atcatcgggg tcggggccat gggcgaggcc ctgatgtctg ggttgtttcg cgccgggtgg
      661 gagcccgccg acgtttcgct ctgtgtgcgt cgacctgatc gggcgcaaag cctcacggag
      721 atctccggcg ctctcgtctc gtccgatcca attgagacca tccgcgaccg tgacgtggtg
      781 gtggtggcag tcaagccacg cgatgtcccc tctctcctcg atcatctctc cggtcacgtc
      841 gggcctgagc agatggttct ctcccttgcc gccggggtga ccacggggac ctacgagtcg
      901 cgactcggtg ccgtcccggt agtgcgtgcg atgccgaaca ctcccgcctt ggttggcgaa
      961 ggcgtcaccg ggatcgccgg cggtgccaac gccggatcgg atcatctcga tttggccggt
     1021 gagatcctgg gcgcggtcgg atcgatccgg atcatggatg agagcctcct cgacgcggtc
     1081 accgcggtct ccggcaccgg tcccgcctat gtcttcctcc tcgcagaggc tctgaccgag
     1141 gcggcggttc gagaagggct ccctagggat atcgccgaga acttcgtcca ccagacgatc
     1201 cggggtgccg gccatctgct gacgagcacc gggaagacgc cggccgagct tcgttcccag
     1261 gtgacctcac ccggcggcac caccgcggct gccatgcacg tcctcgagga gcgaggtttc
     1321 cgcgcgctgg tggaggatgc cgtcgccgcc gcggcgaggc gttcgaccga gctcgggggg
     1381 aaaagctgac cgtttcggcg gggtagcagg actcggaggc aggcacttct acactcgccg
     1441 ccatggccct cacccaggag cgtcaggacg aggccacgac ctctaagcgc ccatccgttt
     1501 cctatgtgat cgagggtctt ggcgtggtcg gggctctggc cacgcttgca ctgggcttgc
     1561 tcggcacgat tcccgaggaa cctgaggtcg aaatcgggcg ggcggtgttc ggcaacatcc
     1621 ctgctgcggt ccagttcgtc ttctacgtca cggtctccgt attcatctgg gtttcggccc
     1681 atctcctggc ccgcagagcg gcgtcctggc agcggggccg gcccgagtcg cgagtcgggc
     1741 tctgggggcg tcgcatcaag gatctcgatg ccgggctgcg catgaagacg ctgatgcgtg
     1801 acccgcgggc cggtgtgatg cactcgctga tctactacgg gttcatcgtt ctctttttgg
     1861 gaacggtcac tctcgagatc gaccatctcc tccctgcgaa tctcaagttc ctccagggtc
     1921 cggtgtatca gggctactcg gcgatcctcg atttggccgc tctggcctat ctcggcggct
     1981 tggccctcgc catcgccaac cgctacctgc tgcgcagtcc tcgtctccgg accaagacca
     2041 agccggaaga cgcgttgatc ctgggcctat tggtcttgct gggcgtgagc ggcctgatca
     2101 ctgaggcagc ccgcatctcg ctcgggggac gaccggactt cgaggtgtgg tcgttcgtcg
     2161 ggtacccgct ctccgccctg atccccgctg cgagcgccag tggcttgcac caggcgttct
     2221 ggattggcca cgtggtcggc ttcgtcgcct tcctcgtgat ccttcccgtc accaagctcc
     2281 ggcacatggt caccgctccg gccaatctct tcctatctgc cggaccccgg cccaagggag
     2341 cgatgaggga gatgcccaat ctcctggaag cagaggacat cgacacgatt ggggcctcgc
     2401 ttgcggccga cctcacctgg aagcagatct tcgacactga tgcgtgcacc atttgtggcc
     2461 ggtgcacttc ggtctgcccg gcgaatatca ccggcaagcc cctcgatcca agagagatcg
     2521 tcctcaagac cggggaggtc gcggcggcgg cggccggggt cagcccggca ggctcatcga
     2581 tcttcttgct cgaaggcgag ccctcgctgt tcgaccgggt tcgacccgag gaggtgttcg
     2641 cttgcaccac ctgccgggcc tgtgacgaga tctgcccggt gaacatcgaa atcgtcgatc
     2701 gaatcctcga catgcgccgc tacctgacgt tgatggactc cgacttccca agcgaattgg
     2761 gcaaggcgtt cgtcgccatg gagaaccagt cgaacccgtg gggcatgagc caacagaatc
     2821 gtgcggcctg gaccgaggag ctcgacttcg aggtgccgat cttcggggag aacgggaaga
     2881 caaccgccga gtacctctgg tttgtcggct gcgccgggtc attcgacgac cggaacacgg
     2941 cggtctcggt ggctctggcc cgattgctca acaaggcggg aatcgacttc gccatcctcg
     3001 gccgggccga gctctgcaat ggcgacccgg cccgccgtgc cggcaacgag ttcgtcttcc
     3061 aacagctggc cctgcagaac atcgccaact tcgatgagat cggagtgaag aaggtgatca
     3121 cccagtgcgc ccactgcttc aacacgatct ccaacgagta tccacagttt ggtgggcatt
     3181 atgaggtggt gcaccacagc cagcttctcg ccgagctact gagccagggc cggatcaccg
     3241 tgcccctcaa ggcgaacggt cagccgaaga agattgccta tcacgacccc tgctatctgg
     3301 gaaggcacaa cgacgtctac ctggcaccaa ggaatgtcat tggggcatcg ggcgacatcg
     3361 agttggtcga aatgccccga cacggcaccc gggcgctgtg ctgcggtgcc ggtggcgccc
     3421 gcttctggat ggaggagcaa accgggaaga aggtcaacat cgagagggct gaggaggctc
     3481 ttgccaccga tgccgacgag atcgccgtcg cctgcccctt ctgttacgtc atgctcgacg
     3541 acggggtcaa ggagttgggc cgggacgacg tcccggtgag ggacctggcg atgatcctcg
     3601 ccgacggagt tgggccgtag cccggagcaa tctcattcga cgccttgccc cgctgttcgc
     3661 cgtgcttgcg ctgctcgccg catgcactcc cgaagccggt ggcccttcgg gcaccgactc
     3721 tcagtcgaca tcgaccacga cgccgaccac cacctcgacc acgacgatca ccggagtcgc
     3781 cgtggaggag ttccgtacct gtctcgcaga gcacgacgtg gacatcgagg aggtgcccct
     3841 cgacgcaacc gggcgtccac gcctcgatct agtgatcgtg agtgtcgact tcggcgaccc
     3901 cgaagtcgct gacgcccttg ccgtctgttc cgcaagtctc aacaccgggg ccctggacct
     3961 gggtgacgac gaggagttgc gtcgcgaggt caatgacgag ttgttggaat tcagcaagtg
     4021 catgcgagcc aacggggtcg aggacttccc cgacccggtt ccggggttcg caggggttgg
     4081 cgctccattc gcggtggcgg agatccccta tggggatccc gagctggcca ctgcgtcggc
     4141 cgcttgcagg gcgtccgtgc tcggaggtct ccccgggttg gacggatcct gacctggcgt
     4201 acgattcggc cgtgacgact cgccccagcc ccgtcatcac cccggaaacc ggtgaatcga
     4261 ccgacgtcga cctcccctgg caagtcatcg tctggaacga cccggtcaac acgatggcct
     4321 acgtggtcta cgtgttccgc aaactcttcg ggtacgacgt ggaaaaggca accaaattga
     4381 tgttggaggt ccatttcgag ggccgttcac gggtgtccga cgggtccagg gagaaggctg
     4441 agatcgactg ctttcgactt cacgggcacg ggttgtgggc gacgatggag caatgagtcg
     4501 cggtcgcttc cgctataaga gcggcaagat cgtggttgag ctcccgaaag aggaaaggga
     4561 gttcctctcc gacgtgctcg agcttttgag ctcgattcca tcaagtggtg acgatcccgc
     4621 gacccgccgt cttcaggtcc ctgtgtatct cgatgatcct gaggcgaatt cggagtggtg
     4681 gctcttcatg ggcgaggagc tttcgattgc ccgctccgcc gatcgggacg tctatcagcg
     4741 ggttatggac tcagaaaaga gaatcacatt gaccacggag gaggccgacg ccttcctgcg
     4801 ggtgctcaat gaggggcggc tcgccttggg agcccgcttc ggtctcgagg tcgaagcaga
     4861 tcacgaccag cttccggagg agcagcgcca ggttctggac tacctgggtt ggaccctcga
     4921 ggagatgacc gtggctctct ccgcagcgct ctgaccgtag ggccgcgtcg aggagtaggt
     4981 ttcgcgaggc agtgactcct cgagtgcctg agcccgccac cgacatccaa catgctggct
     5041 tggcgaggag gcatctgttg ccttcagatc cgattgccgg gctgaggagc ggtgtcggag
     5101 ccttgatcac tatctatctg aaccggcccg gcccgggtgg aatggccgct ctcctcacgg
     5161 acctgttgaa gccggtgcgg gacgaagccg agcgaatcgg aggcttcgtt cgcaagagcg
     5221 tgcgggccga tgcaacgcga atccacgagc tcgccaacca actcgaaggg gatgtggcgc
     5281 ccgccttcgc catcttcgct gcggacgtcg atggactctt cgtgctccaa ccgctcgcct
     5341 attccgttcc caacgtctcc acccttgggc cacggcccta cctgcggcca cttcgtgccg
     5401 cgccgcgacc gctgcgagca ggcatcatcg tcgccgaccg ggcgtcagct cgcatcttca
     5461 tcggctcaga cgacctggtc gaagaactag ccgcctattc ggtggacatc gggaagccga
     5521 actacggggg ctttggcggc tacgaggagc acaacgcaag ggcccatgcc ggcgaggaga
     5581 cgaaccggat gtggaaggaa gccggcgc
//
LOCUS       DBNHXU010000028         3202 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4571810, whole genome shotgun sequence.
ACCESSION   DBNHXU010000028 DBNHXU010000000
VERSION     DBNHXU010000028.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3202)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4571810"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..369
                     /locus_tag="AC161X_00810"
     CDS             <1..369
                     /locus_tag="AC161X_00810"
                     /inference="COORDINATES: protein motif:HMM:NF046506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIR4988117.1"
                     /translation="VLGGGWDTLWVRSFPARHPSLAIGLRLRVPSSWGEESLKVSVEL
                     QDADGAAMLPQPLAHVVTLPGHSQPSSTTDFGLIRSFTFNNLLFRKEGSYSFVISVDD
                     EPVSRLRFAVRARVEAGEPA"
     gene            519..1106
                     /locus_tag="AC161X_00815"
     CDS             519..1106
                     /locus_tag="AC161X_00815"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR4988118.1"
                     /translation="MIAIIAVTALVPPSSAAAAEGPPFQIRFPQETQPTSFSSTFGAR
                     RSGGRRHHGVDLMAPKMTQVYAAADGYVSRIETSSLAGRYLELTHVAGWTTKYVHLNN
                     DTPGTDDGDADWSSTLAPGIVIGAWVEAGQLIAWVGDSGNAEWTGSHTHFELAVDGIP
                     IDPYPFLKLAWARDQSRYRADVWDLGHPLDVYRVF"
     gene            complement(1117..1554)
                     /locus_tag="AC161X_00820"
     CDS             complement(1117..1554)
                     /locus_tag="AC161X_00820"
                     /inference="COORDINATES: protein motif:HMM:NF017206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NmrA family NAD(P)-binding protein"
                     /protein_id="HIR4988119.1"
                     /translation="MPVIVIGADTGAGLAISTELANPGREVRAFVSDPVIAGDLKRMG
                     VKVALGDVSDDSHIEAAAMHCFTAILVTEAARDDRERSFAKSEADVLEAWARAVSNAK
                     VNRVIWVYDGAAPEVEVSEVATVSSSDPNLARRVAELDDARVI"
     gene            1713..3122
                     /gene="sufB"
                     /locus_tag="AC161X_00825"
     CDS             1713..3122
                     /gene="sufB"
                     /locus_tag="AC161X_00825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HIR4988120.1"
                     /translation="MATVDLDLGSYRLGWHDDVEYAFTPRKGLDQGVIREISALKGEP
                     EWMLAFRLKAYDRFLKKPMPQWGGGGLLNTIDFDDIYYYIKPAGEQQKDWDMVPEGIK
                     ATYEKLGIPQAERKYLAGVTAQYESEVVYHRNREDLEKLGVLFTDMDTALREYPDIVK
                     KYFGTIIPPNDNKFAALNSAVWSGGSFIYVPPGVHVDQPLQAYFRINSENMGQFERTL
                     IIVDEGAYVHYVEGCSAPVYTTDSLHAAVVEIIVKEGARCRYTTIQNWSNNVFNLVTK
                     RAVAYRNATMEWVDGNLGSRLTMKYPAVWLMEPGAHGEVLSIAFAGEGQHQDAGAKMV
                     HVAPDTTSTIVSKSIAKGGGRSGYRGLVRVEPGAENAKSFVRCDALILDENSRSDTYP
                     YMEIEENDAEIGHEATVSKVGEEQLFYLMSRGLTEDQATSMIVAGFIEPIVKELPMEY
                     AVEMNRLIELNMAEAGAVG"
BASE COUNT          612 a         1006 c          965 g          619 t
ORIGIN      
        1 gtgctgggtg gcggctggga caccctttgg gtccgctcgt ttccagcccg tcatcccagc
       61 ctggcgatcg gtcttcggct ccgcgtccca tcttcctggg gcgaggaatc gttgaaggtg
      121 tccgttgagc ttcaggacgc ggatggcgct gcgatgctgc cccaaccact agcccacgta
      181 gtcactctcc ccggtcacag ccagccatcc tcaaccaccg acttcggctt gatccgctcc
      241 ttcaccttca acaacctgtt gttccgaaaa gagggctcct actccttcgt catctctgtc
      301 gacgacgagc cggtgtcacg gctccgcttc gcggttcggg ctcgggtcga ggccggcgag
      361 cccgcttgat gacgcgaatc ggtgtttgca tagcgcgagc accttcgtag cctgccgacc
      421 tcgcacggga caacacgtac gtcccgactc ttcccctatc tacggaggtc attctgtttt
      481 cgctgcaaca tgctttgccg cgcctcgcgg gaggcttcat gatcgcgatc atcgcggtga
      541 ctgctctcgt tcccccgtcc tcggctgccg cggccgaagg cccgccattt cagatcaggt
      601 ttcctcaaga gactcagccg accagcttct cctcgacatt tggcgcccga cggtcagggg
      661 ggcgccgtca tcacggcgtg gatctgatgg ctcccaagat gacgcaggtg tacgcggcgg
      721 ccgacggcta cgtgtcgagg atcgagactt cttcgcttgc cgggcgctat ctggagctca
      781 ctcatgtcgc cgggtggacc actaagtatg tgcacctcaa caacgacacg ccgggcactg
      841 acgacggcga cgctgactgg tcgtccacgc tggctcccgg gatcgtcatc ggggcatggg
      901 tagaggccgg gcagctgatc gcatgggttg gcgactccgg caacgcggag tggactggct
      961 cccataccca tttcgagctt gctgtcgacg gcattcccat cgacccctat ccatttttga
     1021 agctggcttg ggcccgggat cagagccgat atcgggcgga cgtctgggat ctcgggcatc
     1081 ctctggacgt ttatcgagtt ttctgagtct gatcagttag atcacgcggg cgtcatcgag
     1141 ctccgcaacc cggcgcgcca ggtttgggtc tgaggaggag actgtcgcga cctccgacac
     1201 ttcgacttcg ggcgcggcgc cgtcgtagac ccaaatcacc cgattgacct tcgcattgct
     1261 gactgcgcgt gcccaggcct cgaggacgtc ggcttcgctc tttgcaaagg atcgctcacg
     1321 atcgtcccgg gccgcctcgg taacgagaat ggcggtgaag cagtgcattg cggcggcctc
     1381 gatatggctg tcgtcggaga cgtcacctaa ggccaccttg acccccatgc gcttcagatc
     1441 tccagcaatc accggatccg agacgaaggc ccgaacttca cggcccggat tggcaagctc
     1501 ggtgcttatg gcgagcccgg caccggtgtc ggcgccgatg acgatcacag gcatgggccg
     1561 aacctaatgg atccggaaca aagccccgga ccgctgatgt tgattatgtc agtggctggc
     1621 atgggctttc atccagcccc atgcttccgg tacaatcgca ctaccgggat agtggaaatt
     1681 aattcagccc acaggaattg gtgattactg aaatggcaac tgtcgacctc gatctgggtt
     1741 cgtaccgcct cggctggcac gacgatgtcg agtacgcctt cacgccgcgc aagggcctcg
     1801 atcagggcgt gatccgcgaa atctccgctc tcaaaggaga gccggagtgg atgctcgcgt
     1861 tccggctcaa ggcttatgac cgcttcctga agaagccgat gcctcaatgg ggcgggggcg
     1921 gcctgctcaa caccatcgat ttcgacgaca tctactacta catcaagccg gctggcgagc
     1981 agcagaagga ctgggacatg gtccccgagg ggatcaaggc cacctacgaa aagcttggga
     2041 tcccgcaggc ggagcgcaag tacctggccg gtgtcaccgc tcagtacgaa tccgaagtgg
     2101 tgtaccaccg caaccgggag gacttggaga aactcggcgt cctgtttacc gacatggaca
     2161 ccgccctccg cgagtacccc gacatcgtca agaagtactt cggcacgatc atcccaccca
     2221 acgacaacaa attcgccgcc ctgaactcgg cggtgtggtc tggtgggtcg ttcatatatg
     2281 ttccgccggg ggttcacgtc gaccagccgc tacaggccta cttccggatc aactccgaga
     2341 acatgggcca gttcgagcgg acactgatca ttgtggacga gggcgcgtat gtgcattacg
     2401 tcgagggctg ctcggcgccg gtctacacca ccgactccct tcacgccgcg gtggtcgaga
     2461 tcatcgtcaa ggagggcgcc cgctgtcggt acacgaccat ccagaactgg tcgaacaacg
     2521 tcttcaacct ggtgaccaag cgcgcagtcg cctaccgcaa cgccacgatg gagtgggttg
     2581 acgggaacct cggaagccgc ctcaccatga agtaccccgc tgtctggctg atggagcccg
     2641 gggcccacgg tgaagtgctc tccattgcat tcgctggaga gggccagcac caggacgccg
     2701 gagccaagat ggtccatgtc gcacccgaca ccacctccac gatcgtgtcc aagtcgatcg
     2761 ccaagggtgg gggccggtct ggttatcggg gactggtccg ggtcgagccc ggtgccgaga
     2821 atgcaaagag cttcgttcga tgcgacgccc tgattctcga cgagaacagc cgctcagaca
     2881 cctatccata tatggaaatc gaagagaacg acgccgagat cgggcacgaa gccaccgtgt
     2941 ccaaggtggg tgaggagcaa ctgttctatt tgatgagccg gggcctcacc gaggatcaag
     3001 ccacatcgat gatcgttgcc ggcttcatcg agcccatcgt caaggagctg ccgatggagt
     3061 atgcggtcga aatgaaccgc ctcatcgaac tcaacatggc ggaggcgggt gcagtcggtt
     3121 aaacggtggc cgccgacggc taggtgttgc tgcggccgac gacgtagccg aggatgaggc
     3181 cgccgacggc cccccacacc ac
//
LOCUS       DBNHXU010000029         6239 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4374686, whole genome shotgun sequence.
ACCESSION   DBNHXU010000029 DBNHXU010000000
VERSION     DBNHXU010000029.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6239)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6239)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6239
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4374686"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..317)
                     /locus_tag="AC161X_00830"
     CDS             complement(<1..317)
                     /locus_tag="AC161X_00830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988121.1"
                     /translation="MTTLIERPWTEVTRVPRRSMVVESTIGIVGVLAAAVGAWMYYVP
                     ADWLLGGLAAGWYLGMFVGAGVLLATAFGLYARSAFKADGAWTTRVTFATMLGVAAAI
                     GAVT"
     gene            485..1387
                     /locus_tag="AC161X_00835"
     CDS             485..1387
                     /locus_tag="AC161X_00835"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR4988122.1"
                     /translation="MTIPDPYWPRADVWLNQEHPDPDVLVVGIPSSKASLSLSRADLS
                     PLAMRDRLARFSTFHGEWGVDFGEVAVNDYGNWPVSELDRDQMPILVEELASALPKVP
                     LTLYLGGDNAITRPLVAAMAPDLARVGVLTFDAHHDVRTLELGRTNGNPIRGLVDEDG
                     LPGPNVTQIGIHSFANSAEYRAYCDRVGIKVVTISDIETGGISEVTNLALASLSERCD
                     AIYVDVDVDVLDRAFAPACPGARPGGMTLRDLAEAVRRCARDPKVKAMDFVEVDAEAD
                     RDGLTLDSMAHLMLSAVAGYAERG"
     gene            complement(1384..2496)
                     /gene="dusB"
                     /locus_tag="AC161X_00840"
     CDS             complement(1384..2496)
                     /gene="dusB"
                     /locus_tag="AC161X_00840"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HIR4988123.1"
                     /translation="MLRIGPLTVELPVVLAPMAGVTNAPFRRLCREFGAGLYVSEMIG
                     ARGLVEGDEKTLALAAFDADESPRSIQLYGTDPGSLGEAVRRLVEVDQVDHIDMNFGC
                     PMGKVTRHGGGAALPWKRDLFRSIVRAAVDNAGTVPVTVKFRKGIDDEHLTFIETGLT
                     AADEGAAAVALHARTARQLYSGAADWDAIRALKEAVTSVPVLGNGDIWTADDAVRMME
                     MTGCDGVVVGRGCLGRPWLFRDLADRLSGREPGPAPNLGEVALIMIAHAQLLAEKFGE
                     GIGVSLFRKHTAWYLKGFPAGPSIRDRFARVGSLDEVVALTGLLDPTVPFPKEAERMV
                     RGHSHGPRPVRLPHGFLDHRDGDSLGREAEQLISGG"
     gene            complement(2509..2919)
                     /locus_tag="AC161X_00845"
     CDS             complement(2509..2919)
                     /locus_tag="AC161X_00845"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011209055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0047617 - fatty acyl-CoA hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HIR4988124.1"
                     /translation="MENPFEDRTTFVGHSGIELTDASADHATGRIQITENHHQPYGVV
                     HGGVYCTLVETLTSTGGAIWAIENGMAGVVGINNQTDFIRATREGVLLGEATPIHRGR
                     TQQLWQCVVTREEDGKLVARGQVRLQNIKDLEGL"
     gene            complement(2920..3906)
                     /locus_tag="AC161X_00850"
     CDS             complement(2920..3906)
                     /locus_tag="AC161X_00850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988125.1"
                     /translation="MRRWTIALSVLLGVIVVVLIFMFTRDDAGETTTTEFGATTTSTA
                     LGSTTTVSDGTTTTSDESTTTTQEATTTSIAGGLEGNWAEEPLVAAGFGGLGWWDGGN
                     WVQVDEGTSLPVSGGEDYQIAFLGVEARTTGGGEELLCEPLNNPGVVLADEDLLGDWP
                     GPFGVAISADWDLVPNLVEETVDDGTYAAIASQLLAERGLTVANPPIKQVVRVDLEGD
                     GVNEVLVVSEDVPDGLLAEVGDYSIAFLQKVVDGAPATSVIGESVVVELAEGETPFVL
                     SFSIAAVADLSGDGRMEIVLDSAYYEGVGIEVWEYVNDDLGPMAQISTGCGA"
     gene            complement(3916..4509)
                     /locus_tag="AC161X_00855"
     CDS             complement(3916..4509)
                     /locus_tag="AC161X_00855"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012302559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009146 - purine nucleoside triphosphate
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HIR4988126.1"
                     /translation="MRLVLASGSPRRRALLETLRLDFDVEPADVDETRLPDEAPALYV
                     ERVARAKAETVARLDRLVIGADTTVVHEGRVMGKPGHPEEARAMLRRLQGETHEVLTG
                     LAVARFDSELSIQAVVDVAMVEMVPMTEDEIADYVGTGEPMDKAGAYALQGMGGVFVR
                     RVIGSPFTVMGLPIHLLDRLMSAAGAGLSHFRIQDSG"
     gene            complement(4506..5570)
                     /locus_tag="AC161X_00860"
     CDS             complement(4506..5570)
                     /locus_tag="AC161X_00860"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR4988127.1"
                     /translation="MRFVLRPPGGEPLIRDIAGDSWVKPSGSADSTFGQGMWAIPGLV
                     DGHAHFAAVERVDWVTDGVAGATERARDALAAGVMLALDKGWSDLTTMEMIRSTGPEA
                     RPDIEAAGVINAVEDGYWPDFGRRVAPSEVARVVTEAALEGEGWVKLVGDWPRRGVGP
                     MPNFTEAELVEAVEAAGRLGARVAIHTMAREVPSMAVRAGIQSIEHGLFLTEDDLGAL
                     GARGGMWVPTILRMEAVIGQLGAESSGGRLLREGIENAAGLLGIAVEAGVFVLTGTDL
                     AVGTRAVAEEAVRLWELGLSPERVVQAVSSAGFWATGRAASFEVGTPANVVLYAEDPA
                     RDPRVLAHPTLVVRRGRVMG"
     gene            5618..>6239
                     /locus_tag="AC161X_00865"
     CDS             5618..>6239
                     /locus_tag="AC161X_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988128.1"
                     /translation="MLKSVLVAALLVSACTSGAGQTTTTTPATPTTTSLRPTTTAPPL
                     PVQVDDCSAPQVSFSALCETYQLIQEWHVDRPLDTTALAAAALEGLDNFATDQSADPP
                     RALMCAIPDPAFADLCEHLAARVDSESIPIGPAVEAAVSEMANHGLDQFSYYVPPDQV
                     GAFRSNGVVGGVGALLDATDAVGSHCARVTASCPLRVVFVVEDNPAE"
BASE COUNT         1149 a         2190 c         1836 g         1064 t
ORIGIN      
        1 gtcacagccc caatagccgc agccacaccc agcatcgttg cgaatgtgac ccgggtcgtc
       61 caggccccgt cggccttaaa cgcgcttcgg gcgtagagcc cgaatgcagt tgccagcagc
      121 acgccggctc cgacgaacat gcccaggtac cagcctgcgg cgagtccgcc aaggagccag
      181 tcggcgggca cgtagtacat ccacgcgccg acggcggctg caagcacacc gacgatcccg
      241 atcgtcgatt cgacgaccat tgaccgtcgc ggcactcggg tgacctccgt ccatgggcgc
      301 tcgataagcg ttgtcacgaa gatcacctcc tttcttctgg gtagccctcg tgttcgctac
      361 ccggttcgag tgtcctcaaa tcgacagccg gcgcctagag ccatccggcc cgaaacagga
      421 cgcgttgggc ccttgccacg cctacggaca acgagcatga atccgcggat accctgaacg
      481 gcgcatgacc atccctgatc cctactggcc gcgagccgat gtatggctca accaagagca
      541 tcccgaccct gatgtcctgg tggtcggaat cccctcctcc aaggcgtcgt tatcactctc
      601 gcgcgccgac ctcagcccac tcgccatgcg agaccggctt gctcgatttt ccactttcca
      661 cggggaatgg ggtgtggact tcggagaggt tgcggtcaat gactatggca attggccggt
      721 ttcggaattg gaccgagacc agatgccgat cctggtcgaa gagttggcat cggcgctccc
      781 gaaggtgccg ctgacgctgt atctgggggg cgacaacgca atcaccaggc cactggtggc
      841 ggcaatggcg cccgacctcg cccgggtggg tgtgctcacc ttcgacgccc accacgatgt
      901 gagaaccctc gagctcggca ggacaaacgg caaccccatt cgcgggctgg tcgatgagga
      961 tgggctgcct gggcccaatg tcacccagat cggtatccac agcttcgcca attcggcgga
     1021 gtaccgcgcc tactgcgaca gagtcgggat caaagtcgtc acgatcagcg acatcgagac
     1081 cggcggaatc tcagaagtga ccaacctcgc cctggcctcc ctctccgagc ggtgtgatgc
     1141 gatctacgtc gacgtcgacg tcgacgtcct cgatcgcgcc tttgccccgg cgtgccctgg
     1201 agcacggccc gggggaatga cgttgcgaga cctggcggag gcagtgcgtc ggtgcgcccg
     1261 cgacccgaag gtgaaggcaa tggactttgt ggaggtcgat gccgaagcgg accgcgatgg
     1321 gctgaccctt gattccatgg cccatctcat gctgtcagcc gtcgccggat acgccgagcg
     1381 cggctaaccg ccgctgatca gctgttctgc ctcacggccc agcgagtcac cgtcccgatg
     1441 atcgaggaac ccgtggggca atcgaaccgg gcggggccca tgggagtgac cgcgaaccat
     1501 gcgctctgcc tctttcggaa acggaaccgt tgggtccagc aacccggtta gcgccacgac
     1561 ctcgtccaac gacccgaccc gggcaaagcg gtcgcgaatg ctcgggcccg cggggaatcc
     1621 cttcagatac caggcggtgt gcttcctgaa gaggctgacg ccgatcccct ccccgaattt
     1681 ctcagcgaga agctgggcat gggcgatcat gatcagcgcc acctcgccga gatttggagc
     1741 cggacccggc tcacgtcccg acagccggtc agccaggtcc cgaaacagcc atggccttcc
     1801 gaggcagccg cgaccaacca cgaccccgtc gcatccggtc atctccatca tccggaccgc
     1861 atcatcagct gtccagatgt ccccgttgcc gagcacgggc accgaggtca cggcctcctt
     1921 gagagcccga atcgcatccc agtcagccgc tcctgaatag agctggcggg cggtgcgggc
     1981 gtgcagcgct accgccgctg ccccctcgtc ggcggctgtc agaccggtct cgatgaaagt
     2041 gaggtgctcg tcatcgatgc ccttgcggaa cttgacggtg accgggaccg tgccggcatt
     2101 gtctaccgcg gctcgaacga tggaacgaaa gagatctcgc ttccaaggca gggctgcccc
     2161 tccgccgtga cgggtgacct ttcccatcgg acagccgaag ttcatgtcga tgtgatcgac
     2221 ctggtcgact tccactagcc gtcgcacggc ctcgcccaag gagccagggt cggtgccgta
     2281 gagctggatg gagcgggggc tttcgtcggc atcaaaggcg gctagggcga gagtcttctc
     2341 gtcaccttcg accaggcccc gggcaccgat catctcagag acgtagagcc cggcgccgaa
     2401 ttcacggcac agccggcgaa atggcgcatt ggtgaccccg gccatagggg ccagcaccac
     2461 cgggagctct acggtcaggg ggccgatgcg aagcatatgg ctgtcaggct aaagaccctc
     2521 gagatccttg atgttctgca ggcggacttg gccccgagcc accagcttcc cgtcctcttc
     2581 gcgggtcacc acgcactgcc acaactgctg ggtgcggccc cgatgaatcg gtgtcgcctc
     2641 accgagaagc accccttcac gggtggccct gatgaagtcg gtctggttgt tgatccccac
     2701 cactccggcc atgccgtttt cgatggccca aatcgcacct ccggtcgacg tcagggtctc
     2761 gaccagagtg caatagaccc cgccatggac taccccgtag ggctggtgat ggttctcggt
     2821 gatctgaatc ctgcctgtcg catgatccgc cgaggcgtcc gtgagctcga tgccgctatg
     2881 cccgacgaat gtggtccgat cctcgaacgg attttccact caggccccgc agcccgttga
     2941 gatctgcgcc atcggcccga ggtcgtcgtt cacgtactcc cagacctcta tgcctacccc
     3001 ttcgtagtag gcggaatcga ggacgatttc catacggccg tcaccactga gatccgccac
     3061 cgcggcgatg gagaacgaca ggacgaaagg tgtctcgccc tccgccagct cgacgacgac
     3121 cgactctccg ataaccgatg tagccggtgc tccgtcgacg actttctgaa ggaacgcaat
     3181 cgaatagtcg ccgacttcgg cgagcaggcc gtcgggcacg tcctcggaaa ccaccaaaac
     3241 ctcgttgaca ccgtcccctt cgaggtcgac ccggaccacc tgcttgatcg gcgggttggc
     3301 cacggtgagg ccccgctctg caagcaactg cgaggcgatt gcggcatagg tgccgtcgtc
     3361 gacggtctct tccaccagat tgggcaccag gtcccagtcc gccgagatag ccaccccgaa
     3421 cggccccggc caatccccca acaagtcctc atcggcaaga accaccccag gattgttcag
     3481 tggctcgcag agcagctcct cgccaccgcc cgtcgtcctc gcctcgaccc cgagaaaggc
     3541 gatctgatag tcctcgccgc cggacacggg caggctggtg ccctcgtcga cctggaccca
     3601 attgccgccg tcccaccagc cgaggccgcc gaatccggct gcgaccaaag gctcctctgc
     3661 ccagttcccc tccagaccgc ccgcaatcga ggtcgtcgtc gcctcctgag tcgtcgtcgt
     3721 ggactcatca ctcgtggtcg tggtgccgtc cgaaaccgtg gtcgtgctcc caagggcagt
     3781 tgaagtggtg gttgccccga actcggtcgt ggtggtctcg ccggcatcgt ccctggtgaa
     3841 catgaagatg aggacgacca cgatgacccc gagcaggacg gaaagggcga ttgtccaacg
     3901 gcgcatcggg ccaagttacc cgctgtcctg gattctgaag tgcgacaaac ctgcccccgc
     3961 ggccgacatc aaccggtcga ggaggtggat ggggagcccc atcaccgtga aggggctgcc
     4021 aatcacccgc ctgacgaaaa ccccacccat cccttgaagg gcataggcac cagccttgtc
     4081 catcggctcg ccggttccga cgtagtcggc aatctcgtcc tcggtcatgg ggaccatttc
     4141 gaccatcgca acgtcgacga ccgcctgaat cgagagctcg ctgtcgaatc gagccaccgc
     4201 cagaccggtg agaacctcgt gcgtctcccc ctgaaggcgg cgcaacatcg cacgagcctc
     4261 ttcgggatgc cccggcttcc ccatcaccct cccctcgtgg acaaccgtgg tatcggctcc
     4321 aatcaccagt cgatcgagcc tggcgactgt ttcggcctta gccctggcca cccgctcgac
     4381 atagagagca ggcgcctcat cgggcagtcg ggtctcgtcg acgtcggccg gctcgacatc
     4441 gaaatcgaga cgcaaggtct cgaggagagc acgtctccgg ggagatcccg acgccaacac
     4501 caatctcatc ccattacccg cccgcgcctg accaccagtg tcgggtgggc gaggacccga
     4561 gggtcacgcg ccggatcctc tgcgtaaagc accacattgg ctggtgtccc cacctcgaag
     4621 gacgcagccc ggccggtagc ccagaatccg gccgaggaaa cagcttgcac aacgcgttcc
     4681 gggctcagcc ccagctccca gagccgcacc gcctcctcag caacggctcg ggtacccacc
     4741 gctaaatcgg tcccggtcag gacaaagacc ccagcctcga cagcaatgcc aagcaacccg
     4801 gcagcgttct cgatgccctc gcggagaaga cgtccacccg aggactcagc cccaagctgg
     4861 ccgatgactg cctccattcg gagaatcgtg gggacccaca ttccgccgcg tgcgcccaag
     4921 gccccaaggt cgtcctcggt gaggaacagg ccatgctcga ttgactggat tccggcccta
     4981 accgccatgg aaggcacctc gcgcgccatg gtgtgaatcg caacccgggc gccaagccgg
     5041 ccagcggcct ccaccgcttc cacgagctca gcttcggtga agttcggcat cggccccacc
     5101 ccgcgacgag gccaatcccc gaccagcttc acccaaccct cgccttcgag ggctgcctca
     5161 gtgacgacgc gggcgacctc gctgggcgcg acccttcgcc cgaaatccgg ccaatacccg
     5221 tcctcgaccg cgttgattac gccggctgcc tcgatatccg gacgcgcctc tgggccggtg
     5281 gaccgaatca tctccatggt cgtcaggtcg ctccaaccct tatccaaggc gagcatcacc
     5341 ccggcggcga gcgcatcgcg ggcccgttcg gtggcccccg ccacgccatc ggttacccag
     5401 tcgaccctct cgaccgcggc gaaatgtgcg tggccgtcga ccagtcccgg aatcgcccac
     5461 attccctgcc cgaaggtcga gtccgccgag cctgacggct tgacccagga atcaccggcg
     5521 atatctcgaa tcaggggctc accgccggga ggccgcaaca cgaatcgcat cgacacatgt
     5581 gcgacggtaa ccgccggctc ctgccgataa tgccgtgatg ctcaagtcgg tcctcgtcgc
     5641 cgctctcttg gtttccgcct gcacttcggg cgcgggtcaa acgaccacca cgacccccgc
     5701 cacgccgacc acgacatctc tcagaccaac gacgacagcg cccccgcttc cagtccaggt
     5761 ggatgactgc tcggcgccac aggtctcgtt ttcggctctc tgtgagactt accaactgat
     5821 tcaggagtgg catgtcgatc gtcccctcga cacgacagcg ctggcggcgg cggctctcga
     5881 gggactcgac aactttgcaa ccgaccagtc cgccgatccg ccccgggccc tgatgtgtgc
     5941 gatcccggat cctgcattcg ctgacttgtg cgaacacctc gccgccaggg tggactcgga
     6001 gtcgatcccg atcggccccg cagttgaagc cgccgtgagc gagatggcca atcacggtct
     6061 cgaccaattc tcctattacg tgccgcctga tcaggtcggg gcctttcgat ccaacggagt
     6121 ggttggagga gtcggggccc ttctcgacgc caccgatgcc gtgggctcac attgcgctcg
     6181 agtgacggcc tcgtgcccgc tgcgggtcgt gttcgtcgtt gaagacaacc cggccgagg
//
LOCUS       DBNHXU010000030         8570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4374916, whole genome shotgun sequence.
ACCESSION   DBNHXU010000030 DBNHXU010000000
VERSION     DBNHXU010000030.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8570
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4374916"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..593)
                     /locus_tag="AC161X_00870"
     CDS             complement(<1..593)
                     /locus_tag="AC161X_00870"
                     /inference="COORDINATES: protein motif:HMM:NF014044.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase HerA domain-containing protein"
                     /protein_id="HIR4988129.1"
                     /translation="MDSEPGKFYIGADRETGEHLLYDSADLTTHGVIVGMTGSGKTGL
                     GIGLIEEALLSGVPCLVIDPKGDMGNLLLDFPDLQPEDFRPWIDEAAAAKEGVSPDEK
                     AASTAQAWGDGLSKAEITPDRMAKLRDTTAMTIYTPGSSAGVPLNVLGSLAAPRLDWE
                     TESEVIRDEIEGFVSSVLALAGVNADPISSPEHILLAT"
     gene            729..2525
                     /locus_tag="AC161X_00875"
     CDS             729..2525
                     /locus_tag="AC161X_00875"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988130.1"
                     /translation="MSSDKITPWSLLRDGIRLIWRFVKGHPAAFGLAVFGAALFAGAI
                     IASAWVIGRVTDEVIIPVLDQGSSFVGLLLPAALAILAVAVIKAFGIVMRRTSAGYLQ
                     FRTRQDVRNELLEHQLSLRMSWLNRQSIGDLLAVADTDVDQGTGVLGPLPYATGVSLL
                     LVGTIIMITAIDPWLGLGALIGLIIVIVVDIRGSWVTYGLWEKVQESRGEVASVAHES
                     FDGALTVKSLGRENYVAHHFGIASDLLRDRLIKVNSTWASYQAVIRALPQALIIALVT
                     VGALRIQEGAITAGNVVTVAYLLALLAFPIQLIGFVLWDLAGSLAGWKRVQAVFDADE
                     LMEYGQAQARSDSGAAPIHGETVHFSYDGSGPVLADLDIHLKAGTVLAVVGPTASGKS
                     TLTLLLARLWDPGAGAIHIDGQDLRAFAASELPKEVSYVTQDAFLFDDTVAGNITLGQ
                     AFSETEIKAAARLAGAESFIAALPKGLDTQLGERGTTLSGGQRQRIALARALIRKPRV
                     LILDDATSAVDPSVEAEILRSLRSAALPSTIVVVAYRPASIRLADEVVYVDERRVVAH
                     GRHDELLETTPGYARLVQAYEEDARRMAGEAS"
     gene            2522..4285
                     /locus_tag="AC161X_00880"
     CDS             2522..4285
                     /locus_tag="AC161X_00880"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988131.1"
                     /translation="MSESKASSKMLINAFRVAPALKQGLRVTLLMAMVGQAITVVTPI
                     VIQKIIDDEILAPGGVDMAGVVQKAAVAICALIVGAFVGRAAVHRLIRSSSTGLSDLR
                     TMTFGHLLKRSVLHTESDRRGDLVSQVTSDVTTLEEFMEWGGVGMIIAGSQVLLALAA
                     MAYYQWRLALVALVGVLLYLLMMRWFQKMLARNYDRVRSEVGNSLGVLSESITAIPVV
                     RAYGIESATKVKVADALERRFWSEFKTARFGNILFSSAEIFAGTLTAAIVVVGIIVGT
                     AQGMSAGTLIAFLFLTNLLIEPLQIVVETLEMGQSAASGLRRVLGALDSEIEVAEPVD
                     PDHLRPGGLSARFNDVSFSYEEGAEVLSGVTVDIAVGSHVAVVGETGSGKTTFAKLLV
                     RLIDPTAGSVFIGGVNAAKLPLDELRTRVAFVPQEGFLFDDTVAGNVRYGSPEADDAE
                     IWTSFHELDLGEWVAGLPKGLDTPVGERGGNLSAGERQLVALVRSWIGSPDLLVLDEA
                     TSAVDPALDVALRHAIQRLIEGRTSVTIAHRLATAEGADEVLVFDRGRLAERGHHRDL
                     IAQGGIYAALYHDWAAGTKTV"
     gene            complement(4300..6369)
                     /locus_tag="AC161X_00885"
     CDS             complement(4300..6369)
                     /locus_tag="AC161X_00885"
                     /inference="COORDINATES: protein motif:HMM:NF024929.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR4988132.1"
                     /translation="MPTSRRELRIRHRGRRTLVVLGLFTFAQIATSLPLAASDTTVRW
                     ESFSESPECGEPYTRPPMGSRGGSLPYSEPILGPYGTYFGRTITQVRARLVLWTVPGS
                     GGQRILVHKAILPALQRVSVGLADEAAAGRVYPITRVSAFAARTVSGTRQISRHAMAA
                     SIDINYPQNPYRADGKLITNMPDWFVKVWRDAGFCWGGSWRGSKDPMHFSWMGPKAAP
                     NVNDPLDPIPPLTSIKAFGPPVADIPTVFAPVMERYGFMTADGDGNASPDVVGLRNHP
                     DGAVLDIALGARNFGSCSVARFFIPDSSVVGAEHTLFVDIDGDSRQDLVTINGSQAVV
                     ATRRDDYEHLMTKTTSLDPDAVAVTGGDFDGDHHADLWAVSPDGTLRVFGGDSWDVLL
                     HEQALPNGAPQQIAVGDRDGGNIPEVFALYPAGSSSRVDVLRLNGSWQLIQGVPVALA
                     PAQVTSIAAADYDGDGRADLEIFDSQGRLKPYLGNSSTGRPASGWFVDVSPDCSDPVR
                     LVYTGSFFDDDSSVHVNGIEAMAAAGITKGCNPPFNDKFCPERVLTRAEAATLLARAL
                     SLPNSGQDYFSDDNGHVLEGGINRLAEAGITKGCNPPANDRFCPNRPLSRAEFAAFIS
                     RALSLPAASKDYFSDDKGNVLEGAINRLAEAGITKGCNPPANTHFCPASLTTRAETAT
                     FLTRALG"
     gene            complement(6383..7261)
                     /locus_tag="AC161X_00890"
     CDS             complement(6383..7261)
                     /locus_tag="AC161X_00890"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR4988133.1"
                     /translation="MPRLIRAAGGVVLRPTNKGRLKILVTHRPNYDDWSLPKGKADPK
                     EKPEQTALREVREETGFDCRIIASLGKTRYQVSAGTKEVSWFAMRPLPGSKGFAPNSE
                     VDQIKWLSRKKAASLLDYERDRELVMETDLKNLSKTGTLFLVRHATAGDRSKWTDHDL
                     RRPLNKSGLRQAAAIAKRLAPNGIERIVSSPYDRCTQTMEPLSALTGAPIEVTEALAE
                     EPEVDRTWELIESLIGYNAVLCSHGDVIPAIINRLMWVGLSIESRFYCSKGSTWEVEV
                     DGGKFTTGTYVPPPET"
     gene            complement(7291..7515)
                     /locus_tag="AC161X_00895"
     CDS             complement(7291..7515)
                     /locus_tag="AC161X_00895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988134.1"
                     /translation="MSHIRPVMNPSKHKIADCRRGSHDYGEGQHIGSGIMRRVCQTCA
                     YVTIDLTGTALTSDGMTESQVAARLTTARR"
     gene            complement(7550..8098)
                     /locus_tag="AC161X_00900"
     CDS             complement(7550..8098)
                     /locus_tag="AC161X_00900"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF037785.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR4988135.1"
                     /translation="MSWPRTVAVFGSSQTELDSEEWAEAVSVGARLASAGAAVITGGY
                     GGTMEAVSLGAAQAGGHVIGVTAPSLFPDRVGANPHVHELIEASSLAHRIDLMMGRAD
                     ATLSLPGSLGTATELLMAWNTNHIRRHHEARLFPSAAVGAQWKMVADALVLLIGAEID
                     GIYWANSSTEGVDWIVGQLGVL"
     gene            complement(8095..>8570)
                     /locus_tag="AC161X_00905"
     CDS             complement(8095..>8570)
                     /locus_tag="AC161X_00905"
                     /inference="COORDINATES: protein motif:HMM:NF014799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HIR4988136.1"
                     /translation="DPVIFLEPKVLYRAGRENVPVEHFTIPIGRARIRKPGTDLTLVT
                     YGGMVPVAIEAANRVEASVEVIDLRTLFPWDRETVLESVARTGRLLLVQEPQGSAGVA
                     ADVAAVVAEKALYDLQAPIVRVTGFDVPWPQFAIEKHALIDASRVVAGIEEALRG"
BASE COUNT         1458 a         2673 c         2775 g         1664 t
ORIGIN      
        1 gtggccagca ggatgtgctc cgggcttgag atcggatcgg cgttgacccc ggcgagggcg
       61 aggacagagg agacgaaacc ctcgatctca tcccggatca cttcggactc ggtctcccag
      121 tcgagcctgg gcgccgccag cgagccgagg acgttgagag gcaccccggc cgaggacccc
      181 ggggtgtaga tggtcatcgc agtcgtatcg cgcagcttcg ccatgcgatc aggggtgatc
      241 tctgccttgg agagcccatc cccccaggcc tgggccgtgg aagccgcctt ctcatcgggg
      301 ctaacgccct ctttggccgc cgccgcctcg tcgatccagg gccggaaatc ttcgggttgc
      361 aaatctggaa agtcgaggag gaggttgccc atatcgccct tggggtcgat caccagacat
      421 gggacaccgc tcagcaaagc ctcttcgata agcccgatcc cgagcccggt cttccccgag
      481 ccggtcatcc cgacgatcac gccgtgagtg gtgaggtcgg cgctgtcgta cagcagatgc
      541 tcaccggtct cccggtcggc gccgatatag aacttgcctg gttcgctatc cattgcgggc
      601 caacctaggc cggcgggcct cgtaattcga ctgacccagc cggcgccggg tcgtagaatc
      661 acccctggtt tggtcgggtc atctgacttg acttcaacca aacttgaagt tgtaggtttc
      721 tgagatccat gtcttccgac aaaatcaccc cctggtcttt gctgcgcgac ggaatccgcc
      781 tcatctggcg attcgtcaaa gggcatccgg cagccttcgg gctcgccgtg ttcggtgccg
      841 ccctgttcgc gggcgccatc atcgcctccg cgtgggtgat cggccgggtc accgatgaag
      901 tgatcattcc ggtcctcgac cagggctcca gcttcgttgg tctgctgctc ccggccgccc
      961 tcgccatcct cgccgtggcg gtcatcaagg ctttcggaat cgtcatgcgc cgcacctctg
     1021 cgggatatct ccagttccgc acccgacagg atgtgcgaaa tgagctgctc gaacatcagt
     1081 tgtcgttgcg gatgtcctgg ctgaaccggc agtcgatcgg ggacctgttg gcagtcgctg
     1141 ataccgacgt cgaccaaggc actggcgtac tcgggcccct gccctacgcc accggcgtgt
     1201 cgctcctcct cgtggggacc atcatcatga tcacggcgat cgatccctgg ctcgggctcg
     1261 gagccctcat cggtctcatc atcgtgatcg tcgtcgacat tcgtggcagc tgggtcacct
     1321 atgggctctg ggagaaggtc caggaatcac gtggcgaggt tgcgtcagtg gcccacgagt
     1381 cgttcgacgg agccctgacc gtcaagtcat tgggacgaga gaactacgtc gctcatcact
     1441 tcggaatcgc ctccgacctg cttcgggacc gcttgatcaa ggtcaactcg acctgggcct
     1501 cgtatcaggc cgtgattcgc gccctccccc aagcgctgat catcgccctg gtcactgtgg
     1561 gggcgctccg gatccaggaa ggcgcaatta ccgcggggaa tgtggtcacg gtcgcctatc
     1621 tcttggcgct gctcgccttc ccaattcagc tgatcgggtt cgtgctgtgg gacctggccg
     1681 gcagcctggc cggatggaaa cgggtgcagg cggtgttcga tgcagacgag ctgatggagt
     1741 acggccaggc tcaggcaaga tcggattctg gcgcggcacc gattcacggc gagaccgttc
     1801 acttctccta tgacgggtcg ggtccggtcc tggctgacct cgacatccac ctcaaggcgg
     1861 ggacggtctt ggccgtggtc gggccaaccg cctcgggcaa gtcgacgctc actcttctcc
     1921 tggcccggct ctgggatccc ggcgccggcg cgatccatat cgatggccag gacctgcgcg
     1981 cttttgccgc ttcggagttg cccaaggagg tctcctatgt gacccaggat gccttccttt
     2041 tcgacgacac cgtcgctgga aacatcacat tgggacaagc cttctctgaa accgagatca
     2101 aggcggcagc gcgcctggcc ggcgccgagt cattcattgc cgctcttccc aaaggcctcg
     2161 acactcagct cggggagcgg ggaaccacgc tctcaggtgg gcagcgtcag cgaatcgcgc
     2221 tggctcgggc gctgatccgc aagccaaggg ttctcatcct cgacgacgcc acttcggcgg
     2281 tggacccttc ggtcgaagcc gagatcctgc gcagccttcg ctcggccgct ctgccctcga
     2341 cgatcgtggt cgttgcgtat cgaccagctt cgatccggct cgccgacgag gtcgtatatg
     2401 tggacgaacg gagggtggtc gctcacgggc gccacgatga gctcttggag acgactccgg
     2461 gctacgcacg cctggtccag gcatacgagg aggacgcccg gagaatggca ggtgaggcgt
     2521 cgtgagcgag tccaaggcca gcagcaagat gctgatcaac gcctttcgag tggcgcccgc
     2581 cttgaagcag gggcttcggg tgacattgct catggcgatg gtgggccagg cgataaccgt
     2641 ggtgaccccg atcgtcatac agaagatcat cgacgacgag atcctggcac ccgggggcgt
     2701 cgacatggcc ggggtcgttc agaaggccgc ggtagcgatc tgcgccctca tcgtgggtgc
     2761 gttcgtcggc cgggctgcgg tgcatcgcct gatcaggagt tcctccaccg ggttgtccga
     2821 ccttcgcacg atgactttcg gccacctcct caagcggtcg gtgctgcaca ccgagtccga
     2881 ccggcggggg gatctggtgt cacaggtcac ctccgatgtg accaccctcg aggagttcat
     2941 ggaatggggc ggggtcggga tgatcatcgc cggcagtcag gtgctgctcg cgctcgccgc
     3001 gatggcctat taccaatggc gcctggccct ggtggcactg gtcggggtgc tgctctatct
     3061 cctcatgatg agatggttcc agaagatgct ggcccgcaac tatgaccggg tccggagcga
     3121 ggtcggcaac tcgctgggtg tcctcagtga gtcgatcacc gccattcccg tggttcgggc
     3181 ctatgggatc gaatccgcga ccaaggtcaa ggttgccgac gctctggagc gccggttctg
     3241 gtcggagttc aagacggccc gattcggcaa catcctgttc tcatcggcgg aaatcttcgc
     3301 cggcactctg actgccgcca tcgtggtcgt gggaatcatc gtgggcaccg ctcaggggat
     3361 gagcgcgggg acgctcatcg ccttcctctt cctcaccaac cttctgatcg agcctttgca
     3421 gatcgtggtc gagaccctcg agatgggtca gagcgcggct tctggcctga ggcgggtgct
     3481 gggagccctg gacagcgaga tcgaggtcgc cgagccggtc gaccccgatc atctgaggcc
     3541 gggcgggctc agtgcccgct tcaacgacgt ctccttctcg tatgaggagg gagccgaggt
     3601 cctgagcggg gtcactgttg acatcgcagt cggctcccac gtcgcggtgg tgggggagac
     3661 cggatccggg aagacgactt tcgccaagct gctcgtccgg ttgatcgatc cgactgcggg
     3721 aagtgtcttc attggcgggg tgaacgcggc caagcttccg ctagacgagt tgcggacccg
     3781 ggtggcgttc gttccccagg agggcttcct cttcgacgac accgtggccg ggaacgtcag
     3841 atacggcagc ccggaggccg acgacgccga gatctggacc tcgttccacg agttggacct
     3901 cggtgaatgg gtggcgggcc tgcccaaggg tttggacact ccggtgggtg agcggggagg
     3961 gaatctctct gccggcgagc gacaactggt ggctttggtt cggtcatgga ttgggtcacc
     4021 cgacctgttg gttctcgacg aggcaacctc ggccgtcgac ccggcccttg acgtggccct
     4081 ccgacacgcc atccagcggt tgatcgaagg ccgaacttcg gtgaccatcg ctcaccgcct
     4141 ggcgaccgcc gaaggcgcag acgaggtgct ggtcttcgat cggggccgtc ttgccgagcg
     4201 gggccatcac cgagacttga tcgcacaggg cgggatctat gccgccctct atcacgactg
     4261 ggcggctggc accaagacgg tctgaagctg gcccacgact tagcccaggg ctctggtgag
     4321 gaaggttgct gtctcggctc tggtggtgag gctcgccggg cagaaatgag tgttggccgg
     4381 cgggttgcat cccttggtga tcccggcttc ggcaaggcga ttgattgccc cctccaggac
     4441 gttgcctttg tcatcggaga agtaatcctt ggacgccgca ggaaggctca gggcccgtga
     4501 gatgaaggcg gcgaattcgg ccctggataa gggccgattc gggcagaaac ggtcgttggc
     4561 tggggggttg catcccttgg tgatcccggc ctcggccagc cggttgatcc caccttctag
     4621 aacatgcccg ttgtcatcgc tgaagtagtc ctgcccggag ttgggaagag aaagtgccct
     4681 ggccaaaagt gttgccgcct cggccctcgt gagaacccgc tccgggcaaa acttgtcgtt
     4741 gaacggtggg ttgcaaccct tggtgatccc cgccgccgcc atcgcctcga tcccgttgac
     4801 gtggacgctg ctgtcgtcgt cgaagaaact cccggtgtag accagcctga ccgggtcgga
     4861 gcagtcgggg ctgacgtcga cgaaccaacc ggaggcgggt cggcccgtcg aactgttgcc
     4921 gaggtaaggc ttgagccttc cctgggagtc gaagatctcc agatcggccc ggccgtcgcc
     4981 gtcgtagtcg gctgctgcta tggaggtcac ttgtgccggg gccaacgcca ccggcactcc
     5041 ctggatcagt tgccagctgc cgttgaggcg gagcacgtcg accctcgagc tgctgcccgc
     5101 cgggtagagg gcgaagacct cgggaatgtt gccaccgtcg cggtcaccga cggcgatctg
     5161 ttgcggggcc ccattgggga gcgcctgttc atgaagcagg acgtcccacg agtcaccgcc
     5221 gaagacgcgg agtgtgccgt ccggggatac ggcccagagg tcggcgtgat ggtcaccgtc
     5281 gaaatccccg ccggtcactg ccaccgcgtc cgggtccagc gaagtggtct tggtcatcag
     5341 gtgctcgtag tcgtctcgtc gggtggccac taccgcctgg gatccgttga tcgtcaccag
     5401 atcctgacga ctgtccccgt cgatgtcgac gaagagagtg tgctcggctc cgaccactga
     5461 ggaatcgggg ataaagaagc gggcgaccga gcacgacccg aagttcctcg caccgagagc
     5521 gatgtcgagc accgccccgt cggggtgatt gcggagcccg accacgtcgg gcgaggcatt
     5581 cccatccccg tcggcggtca tgaagccgta gcgctccatc accggtgcaa aaacggtcgg
     5641 gatgtcggcc accggcggcc cgaaggcctt gatcgacgtc aatggtggga tcggatcgag
     5701 gggatcattg acgttgggtg ccgcttttgg ccccatccag gagaagtgca tcgggtcttt
     5761 gctccccctc caggaaccgc cccagcagaa gccggcgtca cgccagacct tgacaaacca
     5821 gtcgggcatg ttggtgatca gcttgccgtc tgcccggtac gggttctgcg ggtagttgat
     5881 atctatcgat gctgccatgg catgtctcga gatctgacgg gtcccactga cagttcgggc
     5941 agcgaaagca ctcaccctgg tgatgggata gacccgtccc gccgccgcct cgtccgccag
     6001 gccgacagac acccgctgta gagcaggcag gatcgccttg tggaccagga tccgctgacc
     6061 gccgctcccg ggcacagtcc agaggacgag gcgggcccgg acctgagtaa tggtgcgacc
     6121 gaagtaggtg ccgtacggtc ccaggatcgg ctcgctgtac ggcagcgacc cgccacggga
     6181 gcccatcggg ggccgggtgt aaggctcgcc gcactctggt gactctgaga agctctccca
     6241 gcgcacagtg gtgtcggaag ctgccaacgg cagagacgtg gcgatctggg cgaaggtgaa
     6301 cagcccgagc accaccaatg tgcggcggcc acgatgtctg atcctcagtt cacgccggga
     6361 cgttggcatg agtcgcagcc tgttaggtct ccggaggagg gacataggtg ccggtggtga
     6421 atttgccccc gtcgacttcc acttcccagg tggagccctt cgagcaatag aagcgggact
     6481 cgatgctgag cccgacccac atcaaacgat tgatgatggc ggggatgacg tcgccgtggg
     6541 agcagaggac ggcgttgtac ccgatcaatg actcgatcaa ctcccaggtg cgatcaactt
     6601 cgggctcttc tgcgagggct tcggtgactt cgatcggcgc tccagtgagt gcggacagcg
     6661 gttccatggt ctgcgtgcag cggtcgtacg ggctactcac gatgcgctcg atcccatttg
     6721 gcgccaggcg cttggcgatg gccgccgctt gacgcaggcc cgacttgttg agggggcgtc
     6781 tcaggtcgtg gtcggtccac ttggaacgat cgccggctgt ggcatggcga accaagaaca
     6841 gcgttcctgt cttcgacaga ttcttcagat cggtctccat gaccagctcg cggtcccgct
     6901 catagtcgag gagagacgcc gcctttttcc gtgacagcca cttgatctga tcgacttcgg
     6961 agttcggggc gaagcctttg ctccctggca gtggccgcat tgcgaaccac gacacttcct
     7021 tggtgccggc actgacctgg tatcgggtct ttcctaaaga ggcaatgatc cggcagtcga
     7081 atccggtctc ttcccgaacc tcacggagcg cggtctgctc cggtttctcc ttgggatctg
     7141 ccttgccttt gggcagcgac cagtcgtcat agttaggtcg gtgggtcacc aggatcttga
     7201 ggcggccctt attcgtcggc cgcagaacga cacccccggc ggctcggatg agtcgtggca
     7261 ccggcgtgag cttagaggct cttgttggtt ttaacgcctg gcggtcgtga gccgggccgc
     7321 aacctggctc tcggtcattc cgtcggatgt gagcgcggtg cccgtgagat caatggtcac
     7381 gtaggcacag gtctgacaca cacggcgcat gatcccggaa ccaatgtgct gaccttcgcc
     7441 gtagtcgtgg ctgcctcggc gacagtcggc gatcttgtgt ttggacggat tcatgacggg
     7501 acggatatga ctcataactg tttcagagac tagggctggt caggttttgt caaagcaccc
     7561 caagctgacc gacgatccag tccaccccct cggtgctgct atttgcccag tagatcccgt
     7621 ctatttcggc gccgatgagc aggactagcg catcggcaac cattttccat tgagccccga
     7681 ctgcagcgct gggaaagaga cgggcctcgt gatggcgcct tatgtgattg gtgttccaag
     7741 ccatcaacaa ctcggtcgcg gttcccaacg agccgggcag cgacagcgtg gcgtcggccc
     7801 gacccatcat caggtcgatg cggtgggcca ggctggacgc ctctatcagc tcgtggacat
     7861 gtgggttggc acccacccgg tcaggaaaca aggacggagc agtcactccg atcacgtgtc
     7921 ctcccgcctg tgccgccccc aaggacacgg cttccatcgt gccgccgtag ccaccggtga
     7981 tcaccgccgc accagcggag gcgagtcggg ctccaacgga gactgcttcg gcccactcct
     8041 ctgaatcgag ctcggtctgt gacgatccga acaccgccac tgtgcgtggc caactcaacc
     8101 tctcaaggcc tcctcgatcc cggcgaccac ccggctggca tcgatcaaag cgtgcttctc
     8161 gatggcgaat tggggccagg ggacgtcaaa gccggtgacc cttacgatcg gagcctggag
     8221 gtcgtacagc gccttctcag ccaccaccgc cgccacgtcg gcggcgaccc ccgccgagcc
     8281 ctgtggctcc tgtaccagga ggagacggcc ggtgcgcgcc accgattcga gaacggtttc
     8341 ccggtcccac ggaaagagag tgcggagatc gatcacttcc accgaggctt ccacccggtt
     8401 ggcagcctcg atggccaccg ggaccattcc tccgtaggtg acgagggtca gatcggttcc
     8461 cggcttgcgg atccgtgccc gaccgatggg gatggtgaaa tgctcgaccg gcacattctc
     8521 tctcccggcc cgatagagaa ctttgggctc gaggaagatc accgggtcgt
//
LOCUS       DBNHXU010000031         1943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2192836, whole genome shotgun sequence.
ACCESSION   DBNHXU010000031 DBNHXU010000000
VERSION     DBNHXU010000031.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1943
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2192836"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..914
                     /locus_tag="AC161X_00910"
     CDS             <1..914
                     /locus_tag="AC161X_00910"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF885 domain-containing protein"
                     /protein_id="HIR4988137.1"
                     /translation="GWSEVHRLRDEMRATAAEIDGSMTVEEVIGFLNTDPAHAAPDHD
                     AFARFVQEIQDTAVQQLNGTHFDVPDPLKRVTVNLAPPGGSLGAWYISPSEDLTRPGS
                     IWYAPGERIRIPYWSEVSTAYHEGFPGHHLQVGMSVVNADQQSRFHRLFIWYSGAGEG
                     WALYAERLMDELGYFEKPEYRLGLLASQLFRATRVVLDIGTQLQLAIPADAPLHAGAT
                     WDYEIGVDYIEKVGLQPRDMAESEVKRYLGWYAQAISYKVGERVILDIRDQVMARDGA
                     AFDRKDFHRRMLDAGAIRLDHLREAMV"
     gene            911..1513
                     /locus_tag="AC161X_00915"
     CDS             911..1513
                     /locus_tag="AC161X_00915"
                     /inference="COORDINATES: protein motif:HMM:NF013756.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HIR4988138.1"
                     /translation="MNKRSFDLTTSEPNDHYKLLTGLVIPRPIGWIGTKVGDGKLNLA
                     PFSFFNAVSSEPPIVLFSAGRHRDRPKDSVAFAEESGVFTVNIVSEELASAMNQSSGT
                     FTPDEDEFVIAGLTPVMGSLVDAPMVGESPANLECRVLTVIDIGIETRMVVGEVVMIH
                     VREDALDGTRVDSEVIQAIGRLSGSSYTTTRDRFELRRPG"
     gene            complement(1520..>1943)
                     /locus_tag="AC161X_00920"
     CDS             complement(1520..>1943)
                     /locus_tag="AC161X_00920"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR4988139.1"
                     /translation="RAGRSLKVPAGGAWVCPVKGASFFNDWGFPRAGGTRFHEGNDLF
                     TEYAAPIRAPVSGTVKFKTGVLGGLQFNLKGADGVEYIGSHLSAKETDGKVSAGDVIG
                     YVGTSGNAAGGSPHLHFGMYLRGLPINPYPTLIANGCK"
BASE COUNT          367 a          596 c          615 g          365 t
ORIGIN      
        1 ggggctggtc agaggtccac cggctgcgcg atgagatgcg agcaaccgct gccgaaatcg
       61 acgggtcgat gacggtcgag gaggtcatcg ggttcctcaa caccgatccg gcgcatgccg
      121 cccccgacca cgatgccttc gcccgtttcg tccaggagat ccaggacacg gcggtgcaac
      181 agctcaacgg gacacacttt gatgtgccag acccgctgaa gcgggtgacg gtcaatctcg
      241 ccccacccgg cggcagtctt ggggcctggt acatatcgcc gtccgaagat ctgacccgac
      301 ccgggtcgat ttggtacgca ccgggtgaga ggatccggat cccgtactgg tccgaggtat
      361 cgaccgccta tcacgaggga tttcccggac atcatctcca ggttgggatg tcggtggtca
      421 acgcagacca acagtcgcgg ttccacagac tgttcatctg gtattcgggc gctggcgagg
      481 ggtgggcgct gtatgccgag cgtctcatgg acgagttggg ctatttcgag aagcccgagt
      541 atcgactcgg actgctggcg agccagttgt tccgggccac acgtgtggtg ctcgacatcg
      601 gaacccaatt gcaattggca atcccagccg atgcaccact tcatgccggg gcgacgtggg
      661 attacgagat cggggtcgac tacatcgaga aggtcgggct ccagccccgt gacatggcgg
      721 agtcggaggt gaagcgctat ctgggctggt acgcacaggc gatttcctac aaggtagggg
      781 agcgggtcat cctcgacatc cgcgaccagg tgatggctcg tgacggtgcg gcgttcgatc
      841 ggaaggactt tcaccgcagg atgctggacg ccggcgccat cagactcgat cacctgcggg
      901 aggcgatggt ttgaacaaga gatctttcga tctgacgacc tccgagccca acgaccacta
      961 caagctgctg acgggcctcg tgataccaag gccgattggt tggatcggaa ccaaggtcgg
     1021 agatggaaag ctcaatctgg ccccgttctc cttcttcaat gccgtatcca gcgagccgcc
     1081 gatcgtcctc ttctcggccg ggcgtcaccg cgaccgaccc aaggactcgg tcgcctttgc
     1141 cgaggagtca ggggtgttca ccgtgaacat cgtctcggaa gagcttgcgt ccgcgatgaa
     1201 tcagtcctcg ggaaccttca cccccgacga ggacgagttc gtgatcgccg ggctgacacc
     1261 ggtcatggga tccctcgtcg acgctccgat ggtcggcgaa tcaccggcca acctggaatg
     1321 tcgggtgctg accgtcatcg acatcgggat cgaaaccagg atggtcgtgg gggaggtagt
     1381 gatgatccac gtgcgagagg atgccctcga tgggacgagg gtcgactccg aggtgattca
     1441 ggcgatcggg cgcctctccg gaagttcgta caccaccacc cgcgaccgtt tcgaactgcg
     1501 ccgaccgggc tgagccgcgc tatttgcagc cgttggcgat cagggtcgga tagggattga
     1561 tcggcaggcc acgcaagtac atcccgaagt ggaggtgagg actgccacct gccgcgttgc
     1621 ccgaggtccc cacatacccg atgacgtcac cggcgctcac tttgccgtcg gtctccttcg
     1681 ccgacaggtg actgccgatg tactccacgc cgtcggctcc cttcaggttg aactggagac
     1741 cgccgaggac ccctgtcttg aacttgaccg tgccggacac cggggcccga atcggcgccg
     1801 cgtattcggt gaacaagtcg ttgccttcat ggaacctggt tcctccggcc ctggggaagc
     1861 cccaatcatt gaagaagctg gcgcctttga ccgggcacac ccatgcccct cccgccggaa
     1921 ccttgagact tcgacctgcc cga
//
LOCUS       DBNHXU010000032         2047 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1400080, whole genome shotgun sequence.
ACCESSION   DBNHXU010000032 DBNHXU010000000
VERSION     DBNHXU010000032.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1400080"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1227
                     /locus_tag="AC161X_00925"
     CDS             <1..1227
                     /locus_tag="AC161X_00925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR4988140.1"
                     /translation="PRVIEEVAMDPGSIAWLLVSSALVLFMTPGLALFYGGMVRSKNV
                     LSMLMSNFFTISIVTVIWVVVGYTIAFGADLGGGLLGGFDFFGLEGVTGDTLLFAVFQ
                     MMFAIITPALISGAVAERMKFSAWVLFVGMWSIFVYPVVAHWGFAADGLLFAWGARDF
                     AGGLVVHVNAGIAALALVFALGPRKGFKEEAMRPHSLPLTLLGTGILWFGWFGFNAGS
                     ALAADANAITAFTTTQIAASVAALGWVVAEWWKTGKPTTLGAASGAVAGLVAITPCAG
                     FVSPLASIAIGLIAGVVCFWAVSLKFRFNYDDSLDVVGIHLVGGIAGSLLLGVFASTA
                     VNEAVGLDGLVFGGTTLILKQLGALAVVLVYGFVASYLLARLTKAITGLRADEKAEID
                     GLDISLHEERAYVLTD"
     gene            1238..1576
                     /locus_tag="AC161X_00930"
     CDS             1238..1576
                     /locus_tag="AC161X_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018436703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HIR4988141.1"
                     /translation="MKLIIAYIKPFKLDEVKESLRSIGVTGMSVGEVRGFGRQAGQTE
                     VYRGAEYQVDFVPKIRIEVAVDDGAASRVVEAIESAARTGEIGDGKILVLAVEEVARI
                     RTGERGADAL"
     gene            1591..>2047
                     /locus_tag="AC161X_00935"
     CDS             1591..>2047
                     /locus_tag="AC161X_00935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988142.1"
                     /translation="MRELRASWSGHQAPGDPADLPRALTEAVDALIVEVAEGLDASDW
                     AVVATGGYGRSEMCLQSDIDIMILGNPAPGVVRQMFYPLWDTGMKVGHSVRSLKEAIV
                     GATESMETLCSLLSARLVLGDPQLLARLESQIALLLRRQRAFVDMLGVEE"
BASE COUNT          335 a          574 c          684 g          454 t
ORIGIN      
        1 ccccgagtaa tcgaggaggt agcgatggac ccaggctcaa tcgcctggct gcttgtttcc
       61 agtgccctgg tgttattcat gacacccggg ctggccttgt tctacggcgg gatggtgcgc
      121 tccaagaacg tgctttccat gttgatgagc aacttcttca cgatttcgat cgtgaccgtc
      181 atctgggtgg tcgttggcta cacgatcgcg ttcggcgcgg acctgggagg tggattgctt
      241 ggaggattcg acttcttcgg gctcgaaggt gtgaccggcg acaccctcct gtttgcagtg
      301 ttccagatga tgtttgcgat catcacacct gctctcatct caggggctgt ggcggagcga
      361 atgaagttct ccgcctgggt cctgttcgtt ggcatgtggt cgatcttcgt ctacccggtc
      421 gttgcacatt ggggcttcgc tgcggatggg ctgttgtttg cctggggggc gagggacttc
      481 gccggtggcc tcgtggtcca cgtcaacgcc gggatagccg ccctggccct cgtatttgcc
      541 cttggcccaa ggaaaggctt caaggaagag gcgatgcggc ctcactccct tcctttgacc
      601 ctcctcggaa ccgggattct ctggttcgga tggtttggat tcaatgcggg ctcggctctg
      661 gcggccgacg ccaacgccat aaccgcgttc accacgacgc agattgcggc atccgttgcg
      721 gcgctgggct gggtggtcgc ggaatggtgg aagaccggaa agccgacgac gctgggagct
      781 gcttccggtg cggtggccgg cctcgtcgcc atcacaccat gcgccggatt cgtgagccca
      841 ttggcttcga tcgcgatcgg cctcatcgcc ggagtcgtct gcttctgggc tgtcagcttg
      901 aagttccgtt tcaactatga cgactcgctc gacgtcgtgg gaatccacct cgtcggcggt
      961 atcgccggct cgctcctgtt gggggtattc gcctccacgg cagtgaatga agcggtgggg
     1021 ttggacggtc tggtcttcgg agggaccacg ctgattctca agcaactagg ggccctggcg
     1081 gtggtcttgg tgtacggatt cgtggcttct tatctcctgg ctcgtctcac caaggcgata
     1141 accgggctcc gtgccgacga gaaggcggag atcgacgggc tcgacatctc actgcacgaa
     1201 gagcgcgcct acgttctgac agactgagga aagaaacatg aagctgatca tcgcctatat
     1261 caagcccttc aaactcgacg aggtgaaaga gtcgctgcgg tccatcgggg tgaccgggat
     1321 gtccgttggg gaagtgcgtg gctttggccg tcaagcgggc cagaccgagg tctaccgagg
     1381 tgcggaatac caggtcgact tcgtaccgaa gatccggatc gaggtggccg tcgacgatgg
     1441 agccgcctcc cgagtggtgg aggccatcga atctgcggca cgaaccgggg aaatcggtga
     1501 cggcaagatt cttgttctgg cagttgagga agtagccagg atccgaactg gtgagagggg
     1561 tgctgacgct ttgtgacggc tagggatcgt ttgcgggagc tgagggcgtc gtggtctggc
     1621 catcaagctc ccggcgatcc ggccgacctg ccccgggctc tcaccgaggc tgtcgacgct
     1681 ctgatcgtcg aagtcgccga aggtctggac gcgtcggatt gggcggtcgt ggccactggg
     1741 ggctacgggc gatccgagat gtgcctccag tccgacatcg acatcatgat tctcgggaac
     1801 cccgcacccg gggtggtgcg ccagatgttc tatcccctgt gggacaccgg gatgaaggtt
     1861 ggtcattcgg ttcgttcgct caaggaggcg atcgttgggg ccacggagag catggagacc
     1921 ctctgctctc tgctcagtgc gcggctggtt ctcggtgacc cgcagctcct cgcgaggctc
     1981 gagagccaga tcgccctgct cttgcgccga caaagagcct ttgtcgacat gcttggcgtt
     2041 gaggagc
//
LOCUS       DBNHXU010000033         3574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1003321, whole genome shotgun sequence.
ACCESSION   DBNHXU010000033 DBNHXU010000000
VERSION     DBNHXU010000033.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3574
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1003321"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..399
                     /locus_tag="AC161X_00940"
     CDS             <1..399
                     /locus_tag="AC161X_00940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR4988143.1"
                     /translation="LIGSFLQEDEEEVIDDPDDFKKVPDADFIEMKLAAFQHRVPSLA
                     VRGQVSGVAGLYTMNREDVHPVVGPSGVDGFWVANGFSGHGFKLAPAVGSMVAQAFTG
                     ETMDFDTDVPMEFFSVDREPIDVAVKNVLA"
     gene            400..1269
                     /locus_tag="AC161X_00945"
     CDS             400..1269
                     /locus_tag="AC161X_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HIR4988144.1"
                     /translation="MRYLNAADTRAALPMPDAIEAMEYAFSGKSETPLRTLVGNSLVM
                     PGRLDDIMAVKVVSVVPGNPAGLVAVFDAEGSPVGLVDGPTLTAIRTGAVCGLATRLL
                     AREDSRTLVMLGAGAMAFDQIEAVRAVRPIERVLIWSRSVEKARALAGLVGGEAVTYA
                     DNAVAEADVISCATPASSPLFDPSLIRPSTHVNAVGAFTPRMAELPPDLLNRAYVVVD
                     DLEAAASEAGDLIQAHRQPDTTLRDLLAGSSPESSSDVTVFKSVGVAAQDVAAGHRAL
                     MNAASIGWGTLLE"
     gene            complement(1295..1510)
                     /locus_tag="AC161X_00950"
     CDS             complement(1295..1510)
                     /locus_tag="AC161X_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018518417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HIR4988145.1"
                     /translation="MCRSIKVLREGSVAAPPEEIEAAARQFVRKISGFSKPAAHNFEA
                     FEEAVHEIAHVSQHLLESLEIRGARSA"
     gene            complement(1521..2345)
                     /locus_tag="AC161X_00955"
     CDS             complement(1521..2345)
                     /locus_tag="AC161X_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988146.1"
                     /translation="MAFRSHVTRALDPRSPSNVAISGLTLLAAATGGVIALTADRTLW
                     LPILAAGATFTSWGLGRELDPDHQATGLLAAVAAGALALLGFDISILVTAAALLAARL
                     VVETTGRRPLPGDLTAMVVVASAVSFTALGWVFGFGLAIAIYLDDRMADFHKNQATLA
                     AIGAAVGASLVATLTDAFPQALPPIRAPLTLALGILALIAVLRDPMDPVSLVDSRKKT
                     LLRADRLHAARTLIGLLAFFGSLVASGDITAEVTVALTLALALGSEELGRLRRRSR"
     gene            2404..>3574
                     /locus_tag="AC161X_00960"
     CDS             2404..>3574
                     /locus_tag="AC161X_00960"
                     /inference="COORDINATES: protein motif:HMM:NF012861.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005247 - voltage-gated chloride channel
                     activity [Evidence IEA];
                     GO_process: GO:0006821 - chloride transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HIR4988147.1"
                     /translation="MSSPLDSLRKLPAVGVLAERQMGGFLAISALVGVGAGLGAAALV
                     VLVRWIQESLLPLAIDDGKAWIFLTVPTGFFVAWLIAKRFAPEVSGDGVPQAVAALEV
                     QGGQMRARVIPLKILATAATVGMGGSAGREGPIVQIGAAIGSVISKRFNLGEDQVRSL
                     VAAGAGAGIGATFNAPIAGMLFALEVILSSFAARHMSAIVIASVTAAITTQILVGSEL
                     AIAGGSYRLGSPLELFLYAGLAILVVAVGIGFLRLVGGLERFVRARDRSLGLLRPIAS
                     GLLVAGLIFLEPRVFGTGQAVTNQMLRGEVSDVWWVLIALALVKAVANALTLNSGASG
                     GAFMPSLFMGSALGSGFALLLAPVWGFSDLSSGAFAVVGMAAMFAVVGRAPLTAIL"
BASE COUNT          567 a         1058 c         1234 g          715 t
ORIGIN      
        1 ttgatcggct catttctgca agaggatgaa gaagaggtca tcgacgatcc cgacgacttc
       61 aagaaggtgc cagatgccga cttcatcgag atgaagcttg ccgcctttca gcatcgggtc
      121 ccttcgctgg cggtacgggg ccaggtctcg ggggtggccg ggctctacac catgaaccgt
      181 gaggatgttc acccggtggt ggggcccagc ggggtcgacg ggttctgggt ggccaacggg
      241 ttttccggcc acggtttcaa actcgcgcca gcggtgggat ccatggtggc tcaggcgttc
      301 acgggagaga ccatggactt cgacaccgat gtgccgatgg agtttttcag cgttgaccgc
      361 gagccgatcg atgtcgcggt gaagaacgtc ctggcctgaa tgagatacct caacgccgct
      421 gacaccaggg ctgctttgcc gatgcccgat gccatcgagg ccatggaata tgccttctcc
      481 ggaaagtcgg aaaccccgct tcgcactctc gttggcaact ccttggtgat gccgggccgg
      541 ctcgacgaca tcatggccgt caaggtcgtt tcggtggttc ccggcaatcc ggccggtctg
      601 gttgccgtct tcgacgccga ggggagcccc gttggcctgg tcgacggccc gaccctaacc
      661 gcgattcgaa ccggtgccgt ttgcggtttg gctacgcgct tgttggcccg tgaagactcc
      721 cggacgctcg tgatgcttgg tgccggagcc atggcattcg atcagatcga agcggtgcgg
      781 gcggttcgac cgatcgagcg agtcctcatc tggtcgcggt cagtcgagaa ggcgcgggcc
      841 ctggccggcc tggtgggcgg tgaggccgtc acctatgccg acaacgcggt cgccgaggcg
      901 gatgtgatct cctgtgccac acctgcgagc tctcccctgt tcgacccatc gctgattcga
      961 ccgtcgactc atgtgaatgc agtaggcgct tttacacctc gcatggccga actgccgcct
     1021 gatctgctca accgcgccta cgtggtcgtt gacgacctgg aggccgcagc ctccgaggcc
     1081 ggggacttga tccaggccca ccgccaacct gacaccaccc ttcgtgactt gttggccggg
     1141 tccagtcccg agtccagctc cgacgtgact gtgttcaaga gtgtgggggt ggcagcccag
     1201 gatgtggcgg ctggccaccg tgcgctgatg aacgccgcga gtattgggtg ggggaccctg
     1261 ctggagtagg ggcgtacggg gtaagcgatg gcctctatgc gctgcgggcc cctcttatct
     1321 ccaaagactc caacagatgt tgtgacacgt gtgcgatctc atggaccgct tcctcgaacg
     1381 cctcgaagtt gtgggcggcc ggtttggaga agcccgagat cttccgcacg aactggcgcg
     1441 ccgcggcctc gatttcctcg ggcggtgcgg cgactgagcc ttcccttaga accttgatcg
     1501 atctacacat gcggtgaaac ttaccggctc cgacggcgca gccgtcccag ctcctccgac
     1561 cccagggcca gcgccagggt gagggcaacc gtgacctccg cggtgatgtc gccgctcgcg
     1621 accagggagc cgaagaaggc gagcagcccg atcaaggtgc gagccgcgtg cagacggtcg
     1681 gctcgaagca gggtcttctt ccgcgagtcc acgagcgaga ccggatccat cgggtcccga
     1741 aggactgcaa tgagggcgag gattcccaag gccagggtga gcggtgctcg aatcggaggg
     1801 agtgcctggg gaaacgcatc agtaagtgtg gctaccagtg aagcgccgac tgcagcaccg
     1861 atcgctgcga gcgttgcctg gtttttgtga aagtcggcca tccggtcgtc gagatagatg
     1921 gcgattgcca gtccgaaacc gaagacccat cccagggcag tgaacgagac agcgctggcg
     1981 acgaccacca tcgcagtcag gtcgcccgga agcgggcgcc ggccggtggt ctcgacgacg
     2041 aggcgggcgg ccagaagggc cgcagcggtg accaggatgc tgatgtcgaa cccgagcagg
     2101 gcgagagcgc cagcggcaac cgccgccaga agcccggtgg cttggtgatc cgggtcgagt
     2161 tcccgcccca gaccccagga ggtgaaggtc gcgccagcgg cgaggatcgg tagccagaga
     2221 gttcgatccg ccgtcaaggc gatgaccccg cccgtggcgg ccgcgagcag ggtgagccca
     2281 cttatggcga cattggaggg cgatctcgga tcgagtgccc tggtcacgtg ggagcggaag
     2341 gccatgttca ccattttgga ccgacggtcg ggatttcgtg gttgtgtcgg taccctgcgc
     2401 tcggtgagta gtccactcga cagtcttcgc aagctgccgg cagtgggtgt cctcgccgag
     2461 cgccagatgg gtggattctt ggcgatctcg gctctggttg gcgtgggcgc aggtctcggg
     2521 gcggctgctt tggtggtctt agtccgatgg atccaggaat cgctgctgcc gcttgccatc
     2581 gacgatggaa aggcctggat cttccttacc gttcccacgg gcttcttcgt ggcgtggctg
     2641 atcgccaagc ggttcgcacc tgaggtgagt ggggatggtg tgccccaggc cgtcgctgca
     2701 ctggaagtcc agggtggaca aatgcgggct cgcgtgatcc ccctgaagat tctggccacc
     2761 gcggccactg tgggaatggg tggatcggcc ggtcgagagg gcccgatcgt tcagattggg
     2821 gctgctatcg gttcggtgat ctcgaagcgg ttcaacctcg gcgaggatca ggttcggtcg
     2881 ctagtggcgg caggcgccgg agctgggatc ggtgcgacat tcaatgcgcc gatcgccgga
     2941 atgctcttcg ccctcgaggt gatcctttcc tcgtttgctg cccgtcacat gtcggccatc
     3001 gtcatcgcct cggtgactgc cgcgatcacc acgcaaatcc tggttggctc ggagctggcc
     3061 atcgcgggtg ggagttaccg gctgggtagc cccctcgagc tgttcctcta tgcagggttg
     3121 gcgattctgg tggtggcggt agggatcggc tttctccggc tcgttggtgg gctggagcgc
     3181 ttcgtgaggg cccgtgatcg atcccttggc ctgctccgtc cgattgcgag tggtctgttg
     3241 gtcgccggcc tcatcttcct cgagccccgg gtgttcggca ccggacaggc agtgaccaat
     3301 cagatgcttc gcggcgaggt ctccgatgtc tggtgggtgc tgatcgcatt ggctctggtc
     3361 aaggcggtgg cgaacgcttt gacgctcaat tcgggcgcgt ccggtggggc cttcatgccg
     3421 tcactgttca tgggctcggc cctcgggagc ggcttcgccc tcttattggc gccggtatgg
     3481 ggattctccg atctcagcag tggggcattt gcggtcgtcg ggatggcggc gatgttcgcg
     3541 gtggtgggaa gggcgcccct caccgcgatt ctct
//
LOCUS       DBNHXU010000034         3111 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1201628, whole genome shotgun sequence.
ACCESSION   DBNHXU010000034 DBNHXU010000000
VERSION     DBNHXU010000034.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3111)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3111)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3111
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1201628"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            295..1689
                     /locus_tag="AC161X_00965"
     CDS             295..1689
                     /locus_tag="AC161X_00965"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR4988148.1"
                     /translation="MKLRRNRTLRGWFGRLRPTVQRKLKVAVPYSVMGILTLVLTYMF
                     SDPPPWLYWLPFTALFVLLEFFAVEVNDRLLQSSSVMVAMTAGVIFAMTPDSDAMFGM
                     ALMGGMALFTPLDFKEKRWFQPLANFGQFVLAGAVAGFLLDVNLGDLTEHTAEVLVQV
                     AMASALAALAYATVQTVLIRRAVKTVFGKDNLQPWSQMHVLFLGQFAMGLLGGLIGAA
                     VLIADRQAVLVLIVGVYAIGHMSLYAFSQLRESHIGSIRGFVKTLEAKDMYTRGHTER
                     VAVFAQMIGEELGFTGTQLEKVRWAALIHDLGKLAVPTELIRKRGRLDDEEYAEMQTH
                     AHLVEEILAEVDFLQPMVEIASGHHSNFDGTGYGGRGHTDGQTPSLEACIVGVADAFD
                     AMTSARSYRMALSQQYALRELRRHSGGQFHPDVVDAFEKALIKTGRNWGAPHVNDEEM
                     ARRLAEEPELTLRG"
     gene            1682..2932
                     /locus_tag="AC161X_00970"
     CDS             1682..2932
                     /locus_tag="AC161X_00970"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR4988149.1"
                     /translation="MANWHPRAVAVVSWLFPLLAAGVGLYAASQAAIDPFSIAVGVTA
                     LVIGSLVGVATSSSGNRFTTGLAVAAAIPFVFTNPETGRVDLAASIATYGIGLAVVWA
                     FGLARGEKHIDLLPTLTRRFAGYVTYATVYSAMRVGAFADVSGSFKDLVPFGVALVAW
                     IAVEVLLRAALVLKPGELSRTYLARSALYDLNVFLGLTLTGALFGELWPELDWWALPI
                     ALLPYSFAHSAFKRFQKTKATYKQTIRALARIPEVSGLGVDGHADRTTELASAVAKEM
                     GMGPSQVEDVEYAGLMHDIGRVSLNEPQIIRMGYTNDDIARWSAEIIAESPYLDRVAE
                     HVRRQYESYRRPGEQTDPDVSVVSRIIKVTAAYDWQVYQQGLSPLQALENLHTGAAYE
                     YDPEVVASVRRILEARGVLSPTRV"
BASE COUNT          538 a         1018 c          941 g          614 t
ORIGIN      
        1 gcctcttttg acatgtgaag agttcccgtg aaaacgggaa ctgggtgaga ccggcgtcag
       61 acgtcggcct gtaagcaatt ggacgccatc tacccaagcg gagcggaacg tctgggcggc
      121 aacacaaagc cggccaaata cgagaaccga aggtgagcgt cacagcatga aggcccgttt
      181 ggtccgaatt ctcgctgctg ccgccagcct cgctgccgtg atagccgcag gctcggcttc
      241 atcccgaatc ggctgatgta aggcgccggc tcaccaccca catcggggtc tcccatgaaa
      301 ctgagaagga acagaacgct tcggggctgg ttcggccgtc tccgccccac ggtgcagcga
      361 aagctcaagg ttgctgtgcc ctattcggtc atgggcattc tcaccctggt cctcacctac
      421 atgttctccg atccgccccc gtggctgtat tggcttccct tcaccgccct cttcgtcctc
      481 ctcgagttct ttgcggtcga ggtcaacgac cgtctcctcc agtcgtcgtc ggtgatggtg
      541 gcgatgaccg ccggtgtcat cttcgccatg accccggact ccgacgcgat gttcggcatg
      601 gctctgatgg gaggcatggc gctgttcacc ccgctcgact tcaaagagaa gcgctggttc
      661 cagcccctgg ccaatttcgg ccagttcgtc ctcgccggtg cggttgccgg gttcctcctc
      721 gatgtcaacc tcggggatct caccgagcac actgccgagg tcctggtgca ggttgcgatg
      781 gcctcggcgt tggcggcctt ggcctatgcg acagtccaaa ccgttctcat caggcgggcg
      841 gtgaagaccg tcttcggcaa ggacaacctc caaccctggt cccagatgca tgtgctcttc
      901 cttgggcagt ttgcaatggg gttgctcgga ggcctgatcg gcgcggcagt tctcatcgcc
      961 gaccggcaag cggtgctggt cctcatcgtc ggcgtctatg ccatcgggca catgtcgctg
     1021 tacgccttct cccagctccg ggaatcccac atcgggtcga tccgtggctt cgtgaagacc
     1081 ctagaggcca aggacatgta cacccggggc cacaccgagc gggtcgcggt atttgcccag
     1141 atgatcggcg aagagctcgg gttcaccggg acccagctgg agaaggtgcg ctgggcggct
     1201 ttgatccatg acctgggcaa gctcgcggtc cccactgagc tgataagaaa gcggggtcgt
     1261 ctcgatgacg aggagtacgc cgagatgcag acccacgccc atctcgtcga ggagattctc
     1321 gccgaggtcg acttcctaca gccgatggtc gagatcgcct ccggtcatca cagcaatttc
     1381 gacggcaccg gctacggcgg ccgcgggcac accgacggcc aaaccccgag tctggaggcg
     1441 tgcatcgtcg gggtcgccga cgccttcgac gccatgactt cggcgcgctc ctaccggatg
     1501 gccttgtccc agcaatatgc cctgcgcgaa ctgcgacgcc actccggtgg gcagttccac
     1561 cccgatgtgg tcgatgcctt cgagaaagcc ctcatcaaga ccggtcgcaa ctggggcgct
     1621 ccgcatgtaa acgatgagga gatggcccgt cgtctcgccg aagagccgga gttgaccctt
     1681 cgtggctaac tggcatcccc gcgccgtggc cgtcgtctcc tggttgttcc ctttgcttgc
     1741 ggccggagtc gggctctacg cagccagtca ggcagcaata gatcccttca gcattgcggt
     1801 cggcgtcacc gccctggtga tcggttcctt ggtcggtgtt gccacttcca gctcgggcaa
     1861 ccgattcacc accgggctcg cggtggctgc agccatcccc ttcgtgttca ccaacccgga
     1921 gaccggacgc gtcgatctcg cggcttctat cgccacctac ggcatcgggt tggctgtcgt
     1981 ctgggccttc gggttggccc gtggggagaa gcacatcgat ttgctcccga ccctcactcg
     2041 tcgcttcgcc ggatatgtca cctatgccac cgtctactcg gcgatgcgtg tcggcgcctt
     2101 tgcggatgtc tcgggaagtt tcaaggacct ggttccattc ggtgtcgcct tggtggcctg
     2161 gatcgcggtc gaggttctgt tgcgggctgc actcgtcctc aagccagggg agctttcccg
     2221 cacctatctg gcgagatccg ccctctatga cctgaacgtc ttcctcgggc tcaccctgac
     2281 cggcgccctg ttcggtgagc tctggccgga gctggactgg tgggcactcc ccatcgcgct
     2341 actcccatac tccttcgccc actccgcctt caagaggttc cagaagacca aggccaccta
     2401 caagcagaca atcagagcat tggcccgaat tccggaggtc tcaggtctcg gggtcgacgg
     2461 gcacgccgac cgcaccaccg agctggcgtc ggcggtggcc aaggagatgg gcatgggccc
     2521 atctcaggtc gaagacgtcg agtacgccgg gcttatgcac gacatcggac gggtcagcct
     2581 caacgagcca cagatcattc ggatgggata caccaatgat gacatcgcac gttggagcgc
     2641 cgagatcatc gctgagtctc cctacctgga ccgcgttgcc gagcacgtga gacgtcaata
     2701 cgagtcatac cggaggccag gtgagcagac cgatcccgac gtctcggtcg tctccaggat
     2761 catcaaggtc actgcggcct acgactggca ggtgtatcag cagggcctgt caccgcttca
     2821 ggctctggag aatctccaca ccggagccgc ctacgagtac gaccccgagg tggtcgcttc
     2881 ggtccgccgc atcctcgagg ccagaggggt cctcagccca acgagggtgt aaccggccgg
     2941 atctgaggca tcagggcttg gatgccgagc ccgcctgggc gtgtgcgccc cgcttgaacc
     3001 aacgtcgcgg cctccgcagc tcgtgctcca ggaagcgccc gagccctacg gcgaggaaca
     3061 cgtcacccaa actgatcact tgtccgatcg tggcgattcg cagcggaatc a
//
LOCUS       DBNHXU010000035         2928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1599470, whole genome shotgun sequence.
ACCESSION   DBNHXU010000035 DBNHXU010000000
VERSION     DBNHXU010000035.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2928
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1599470"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..718
                     /locus_tag="AC161X_00975"
     CDS             <1..718
                     /locus_tag="AC161X_00975"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4988150.1"
                     /translation="FAVDSRVWRPQLESLSDQFTVIAWDAPGAGQSSDAPDWFGIDHW
                     SDCLAAFLDTVGVQRAHVLGLSWGGLLAQVFYRRHPTRVRSLILADTYAGWKGSLPEP
                     VPEERLAASLKDASLPPKAFTSKYVPGMFGELATEDIRSELASIMSGFRPDGFRLMAT
                     ALAQADTRDLLPNIEVPTLLIWGDADTRSPISVAEQFRDAIPGARLAIISGAGHVSNL
                     EEPALFDSEVRRFCQSASNA"
     gene            767..1156
                     /locus_tag="AC161X_00980"
     CDS             767..1156
                     /locus_tag="AC161X_00980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HIR4988151.1"
                     /translation="MVDWDDVRRIALGLPETSERASWGLAHWRVRENLFAWERPLRKA
                     DRIALGDTAPEGPILGVRVGDLGVKEALLSDDPGVFFVTPHFDGYPAVLVRLEEIAAD
                     ELEEVIVEAWLDRAPKRLAKEYLDAMK"
     gene            1153..1644
                     /locus_tag="AC161X_00985"
     CDS             1153..1644
                     /locus_tag="AC161X_00985"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988152.1"
                     /translation="MTITFSRLPDAPFDEVLRLINEPRNTRHMPLFSPFTPDEAKGWI
                     AGKDAQWDEFGYGPWAFYIDDRFVGWGGFQREDDGADFGLVLFPEFWGHGLEITRVAL
                     GKGFNEFEFDEVYALLPLTRNADRAMAKVGFETLGVIDYDGIPFRQYRLGREQWELLQ
                     APE"
     gene            complement(1629..2042)
                     /locus_tag="AC161X_00990"
     CDS             complement(1629..2042)
                     /locus_tag="AC161X_00990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012918821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4988153.1"
                     /translation="MDMKLELMILPVSDPDRAKEFYEKAGFHVDVDHQASEDFRVIQV
                     TPPGSACSVTFGKGISQAVPGSIQGMHLVVKDLEAALAEFSERGVEVSEPFHFGAAGQ
                     TPGLHPERSDYGSYAAFSDPDGNGWLLQEVGHSGA"
     gene            2164..2685
                     /locus_tag="AC161X_00995"
     CDS             2164..2685
                     /locus_tag="AC161X_00995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013078380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3291 domain-containing protein"
                     /protein_id="HIR4988154.1"
                     /translation="MHLAQLNVGRLVDHPDSATVAEFIRALPAINLLGESSPGFVWML
                     KDDAGPGALEQRFPGHEDDELFIVNLTVWTNFESLRHFATRSGHAMYLRRRLEWFEKS
                     AEPTTVLWWIEEGHIPGLDEAAEHLAQLRSDGPTPRAFDMKTTFFPIDDGELASTHTS
                     RQIAEGEISPKVS"
     gene            complement(2668..>2928)
                     /locus_tag="AC161X_01000"
     CDS             complement(2668..>2928)
                     /locus_tag="AC161X_01000"
                     /inference="COORDINATES: protein motif:HMM:NF013499.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OB-fold nucleic acid binding domain-containing
                     protein"
                     /protein_id="HIR4988155.1"
                     /translation="KVAGVVTHRQRPGTAKGVYFLNLEDETGLLNIVVLPDVWAKHRE
                     IVRKSPALVIEGRLEFHDGVTNIVARDFQAIGVQTVQSRDFR"
BASE COUNT          539 a          907 c          900 g          582 t
ORIGIN      
        1 ctttgcggtc gactcccggg tctggaggcc gcaactcgaa agcctgtcgg accagttcac
       61 tgtcatcgca tgggacgctc ccggcgccgg tcaatcatcc gacgccccgg actggtttgg
      121 gatcgaccac tggtcggact gtctcgcagc tttcctcgat accgtcggag ttcagcgcgc
      181 ccatgttctt ggtctttcgt ggggagggct tctcgcccag gtgttctatc ggcgacaccc
      241 gacccgggtg cggtctttga tcctggctga tacctacgcc ggctggaagg gctcgctccc
      301 ggaaccagtg cctgaggaga gactcgccgc ttctctcaaa gatgcttccc ttccgccaaa
      361 ggcattcacg tcgaagtatg tgcccgggat gttcggcgag ttggccaccg aggacattcg
      421 aagtgaattg gccagcatca tgtctggctt tcggccggac ggattcagac tcatggcgac
      481 ggcgttggca caggcagaca cccgtgacct gcttccaaac atagaggttc ccactttgct
      541 gatctggggt gatgccgaca cacgatcacc aatcagcgtc gccgaacagt ttcgcgacgc
      601 catacccggt gcccgtctcg ccatcatctc tggggcgggg catgtcagta acctcgaaga
      661 accggcgctg ttcgattcgg aggtgcggag gttttgtcag tctgcctcga acgcctgaga
      721 ctcatttccg cgaccgatac ccgttccggg tttcccgagg gctgacatgg tcgattggga
      781 cgatgtccgg cggatcgcac tgggtttgcc agaaacgtcc gagagagcca gttggggcct
      841 tgcccactgg cgggtgaggg aaaacctgtt cgcctgggag cgcccgctgc gaaaggccga
      901 tcgcatcgcc ttgggcgaca ccgcgcccga gggtcccatc cttggagtgc gggtcgggga
      961 cctaggagta aaagaggcgc tcctctccga tgatcccgga gtcttcttcg tcacccccca
     1021 tttcgacggt tacccggcgg tgcttgtccg gctcgaggag atcgctgccg acgagctcga
     1081 agaggtgatc gttgaggcct ggttggatcg agccccgaag cggcttgcca aggagtattt
     1141 ggacgcgatg aaatgacgat cacgttctcg cgtctgcccg acgccccttt cgacgaggtc
     1201 ctgcgactga tcaacgaacc ccggaacacc cgccacatgc cgctattcag cccgttcacg
     1261 ccggatgagg cgaagggttg gatcgccgga aaggacgcgc aatgggacga gttcggatat
     1321 ggcccatggg cgttctacat cgacgaccga tttgtgggtt ggggcggttt ccaacgagag
     1381 gacgatggag ccgacttcgg gctcgtccta ttccccgagt tctggggtca cgggctggag
     1441 atcacccggg tggccctggg caagggtttc aacgagttcg agttcgacga ggtctatgcg
     1501 ctcttgccac tcaccagaaa cgccgaccga gccatggcca aggtcggctt cgagactctg
     1561 ggagtcatcg attacgacgg aatcccgttt cgtcagtatc ggctcggacg cgagcaatgg
     1621 gagctacttc aagctcctga gtgaccgact tcctggagca gccagccgtt gccgtcagga
     1681 tcgctgaatg ccgcgtaaga gccgtagtcg gagcgttcgg ggtgaagtcc gggtgtctga
     1741 ccggcggcgc caaagtggaa cggctcgctg acctcgaccc cccgttccga gaactcggcc
     1801 agggctgcct cgaggtcttt gaccaccaga tgcatgcctt ggatcgagcc gggaaccgcc
     1861 tggctgatcc ccttcccgaa ggtgacggag cacgccgacc ccggcggggt gacctggatc
     1921 acccggaagt cttcgctcgc ctggtggtcg acgtcgacgt ggaatcctgc cttctcgtag
     1981 aactccttgg cccggtccgg gtctgacacc ggcaggatca tcaattcaag tttcatgtcc
     2041 attggttctc ccctcgtcag agacacggct acgaaagcat caaccgccct atcaccgaaa
     2101 tctcgagaat gcacgctctg caccccgaac gagcgaaatg tgggtcatgg gtacccttgc
     2161 tcggtgcatc tggcccagct caatgtaggc cgcctggtcg accaccccga ctccgccacc
     2221 gtcgcagagt tcatcagggc gctccccgcc atcaacctgc tcggtgagtc cagccctgga
     2281 ttcgtgtgga tgctgaaaga cgacgcaggt ccgggtgcgc ttgagcagcg cttcccgggt
     2341 cacgaagacg acgagctatt catcgtcaac ctgacggtat ggaccaactt cgagtcgctg
     2401 cgccatttcg cgacgcggtc cggccacgcc atgtacttga gacgtcggct ggagtggttc
     2461 gagaagtcag ccgagccaac cacggtgctg tggtggatcg aggaagggca tatcccgggg
     2521 ttggatgagg ccgccgaaca cctggctcag ctgcggagtg acgggccaac gccacgagcc
     2581 ttcgacatga agaccacgtt cttcccgatc gacgatggag agctggcttc cacccacact
     2641 tcccggcaga tagcggaagg cgagatttca ccgaaagtct cgtgactgca ccgtctgcac
     2701 gccaatcgcc tgaaagtccc tggcgacgat attggtcacg ccgtcgtgga actcgagccg
     2761 gccctcgatc accagggcag ggctcttccg gactatttcc cgatgtttgg cccacacatc
     2821 gggcaacacc acgatgttca ggagaccggt ctcatcctcc aggttgagga agtagacgcc
     2881 cttggcggtg cccggccgct gccgatgggt gaccaccccg gccacttt
//
LOCUS       DBNHXU010000036         7102 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1401502, whole genome shotgun sequence.
ACCESSION   DBNHXU010000036 DBNHXU010000000
VERSION     DBNHXU010000036.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7102)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7102)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7102
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1401502"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..815
                     /locus_tag="AC161X_01005"
     CDS             3..815
                     /locus_tag="AC161X_01005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013224195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR4988156.1"
                     /translation="MKKVRIGAQLHPQQGEYRGLRSAVATAEELGFDLVYNWDHFYPL
                     YGNADGAHFECWTMLAAWAEQTSSIEIGALVTCNSYRNPDLLADMARTVDHISGGRLV
                     VGIGAGWFRRDYSEYGFDFGRAGSRLDALAEALPRIKSRWEKLNPPPTRRLPILIGGT
                     GERKTLRMVAEHADGWHAGFPEHPSELEPKVAALLGWCDRIGRDPGDIEWGIGLEPED
                     KGRVLAEHAETYLEMGFTQFTLGFNGPRWNVDDGVDWLTWRDENNGFPLNID"
     gene            1031..2758
                     /locus_tag="AC161X_01010"
     CDS             1031..2758
                     /locus_tag="AC161X_01010"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4988157.1"
                     /translation="MKRMSNRPRWLLLLFVFALVVAACAGDTESTTTTAAPAETTTTA
                     AQATTSTAAAPSSAMTITYDISPDAVWSDGTPITTADFACTWTATLNTPGSLSTVGYD
                     QITSVSEGSSAQQVVVEFATVYAPYKNLFNPLIPAHVVTDPTCMDVSADFLDGYGVSG
                     RAWQMESWSLDQAILTPNPAYWGDDKPVATRLVQVPRAEDGGIAALQAGEVDFIFPQA
                     FAGITDALNADNIKFVPGYGTNYEGLYFQQGEVRNGPFADDDFRAAFSMSIDRATILA
                     NIYDPIFPGAPLLQCGLWVPTVGPWCQNDQFTDSYDPEGAAALLEGAGWVKDGSGMWA
                     KDGVVPEVKWMINTPNPRREATQALMIPLLAEAGFNVVADNGDAAAVFQQRLPAGDYD
                     LGMYINTASPDPSVTGIMSCDAVPSADNGNQGQNSVFWCNEDASAVMAESDTTIDETA
                     RADLIHQIGQALVDDHVMLPLFQFPNVAAWRTDRLEGDAPGADASNYRAYNNSSQLWT
                     PLASDEIIVGAEQWPECLNPITECANSSWMVWTAAFPVLPGVYDTTADGNFVVTNLMA
                     SDPVVTVNE"
     gene            2836..3816
                     /locus_tag="AC161X_01015"
     CDS             2836..3816
                     /locus_tag="AC161X_01015"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988158.1"
                     /translation="MTRFIALRAMWAIPVLLIVTFLTFWAVKSATDPVASYTRINTRA
                     TPEMIEEYKVKHGLVGSTPEQYVRWLKNFVTLEWGNSIKGNRPVWPDLKAAMVTSLIL
                     GLTASFVGITVGLGIGILSALRQYSKFDTAATGAAFVGISIPPFVSAVLLQVFFAIMI
                     TEWLDLDKPFLPVAGVYPPGHVGFDLGLRIQHMILPVTVIAIQIIAVYSRYMRASLLD
                     VKEADYMRTARAKGISERKVITHHALRNAMIPVVTVAAIDIGAIIGGLIITERIFELH
                     GTGDYLLTALGNGDFPQIMPLMFIIVSSVIVFNLVADVAYAWLDPRIRLG"
     gene            3809..4801
                     /locus_tag="AC161X_01020"
     CDS             3809..4801
                     /locus_tag="AC161X_01020"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988159.1"
                     /translation="MVDVASPRPDTVVVPELDELEGIPVVSKSPRQLAMRRYRHHRAA
                     MISSVILLIITLACVFATWVAPYGENEAIKSTDGGLRNEILVPPNSQFWFGTDSIGRD
                     LYSRVLYGGRVSLFVGIASAISAGLIGTAVGSFAGFRGGRIDNALMRFTDLFIGLPLL
                     VILIIMRLLPEKQPWAATVLGEPGSKRLMVTLITLVSWTATARIVRGVVLSLKEKEFV
                     EAARAVGASDRRIITKHLVPNTIGPIVVSMTFIVAGAIALESTLSFFGFGLDPTQSSW
                     GNLLADAGTSVITGKWWLVFFPAMVLLVTILCINFMGDGLRDALDPKQEQRTTG"
     gene            4798..5775
                     /locus_tag="AC161X_01025"
     CDS             4798..5775
                     /locus_tag="AC161X_01025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988160.1"
                     /translation="MTQSVLSVRDLYVSFPTQEGLVQAVRGVSFDIAEHELLGVVGES
                     GSGKSVTFLAVMGLLPKSAIVTGSIQVGGQELVGRPADEVRKVRGKQVAMIFQDPLTA
                     LNPVHRVGAQVVEMILAHQDISDDEADSKAVELLDLVGIPQPDVRARQFPHEFSGGMR
                     QRAVIAMAIANSPEILIADEPTTALDTTVQAQILDVIQNVQHELGAAVAFITHDLGVV
                     ARIADRVQVMYAGRVTERGEAHDIFLRPTHPYTRGLLTSLPDANIERLVPIPGAPPNM
                     MRPPSGCAFRIRCAYAIDACSGEIPDLRPFGSIETACIRVEEIAAEVSA"
     gene            5733..6812
                     /locus_tag="AC161X_01030"
     CDS             5733..6812
                     /locus_tag="AC161X_01030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988161.1"
                     /translation="MHPGRRDCRGGECLSEPLLEVTDLFKLFEVRQNVGAKVIKQTVH
                     AVSGVSFSLQPGETLGLVGESGSGKSTVGRCILRLLEPTSGSVRFRGQEMTTLSREEL
                     RGLRSEMQIVFQDPYASLDPRMTIGAAIAEPLEVHKVPGDHRAIVGELLELVGLSPDH
                     ATRFPHEFSGGQRQRIGIARALSLRPSLIILDEPVSALDVSIQAGILNLLEDLQTELS
                     LSYLFIAHDLSVVRHISDRIAVMYLGKLMEVGDHKEVYDRSIHPYTQSLLSAVPIPDP
                     TIERTRHRIVLAGDLPSPVNPPSGCVFRTRCPIAFDLCAEEVPELRAHSKDHLAACHA
                     PLLPGQSLADVVASRTPVATTVVDE"
     gene            complement(6813..>7102)
                     /locus_tag="AC161X_01035"
     CDS             complement(6813..>7102)
                     /locus_tag="AC161X_01035"
                     /inference="COORDINATES: protein motif:HMM:NF013431.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HIR4988162.1"
                     /translation="LSEWDGTLRLAATLESPPNGLAMELHTTEPGVQVYSGGHMPPTT
                     GKDGVVYPTRGGICLEAQHFPDSPNQPQYPTVLLEPGGTYRQTTEYRFYSR"
BASE COUNT         1302 a         2232 c         2147 g         1421 t
ORIGIN      
        1 cattgaagaa ggttcgaatc ggagcccaat tgcaccctca acagggcgag tatcggggtc
       61 tgcgatccgc ggtggctaca gccgaggagc tcgggttcga cctcgtctac aactgggatc
      121 acttctaccc gttgtatggc aacgctgatg gagcccattt cgagtgttgg accatgctcg
      181 cggcctgggc ggagcaaacc tcgtccatcg agatcggcgc tctcgtcacc tgcaactcct
      241 atcgaaatcc cgaccttctc gccgacatgg cgcggaccgt cgatcacatc tccggagggc
      301 gactggtcgt tgggatcggg gcgggctggt tccgacgcga ctactccgag tatggctttg
      361 acttcggccg ggccggctcc cgtctcgacg ccttggcgga ggcccttccc cggatcaagt
      421 cccggtggga aaaactgaat ccacccccga cccgacggct cccgatcctc attggcggca
      481 ctggcgagcg caagactctg cgcatggtcg ccgagcacgc cgacggatgg cacgctgggt
      541 ttcccgagca cccgtctgag ctggagccga aggtggcagc cctgctcggc tggtgtgatc
      601 ggatcggccg cgatcccggc gatatcgaat gggggatcgg tctcgagccc gaggacaagg
      661 ggcgggtcct cgcggagcat gccgagacgt acctcgaaat ggggttcacc caattcaccc
      721 tcggtttcaa cgggccgcga tggaacgtgg atgatggtgt cgactggctg acatggcggg
      781 acgagaacaa cggattccca ctcaacatcg attgaggccg cgatatccgt gatttttggc
      841 cagccacggt catgaacagt gtcactgata gtggtttcct gacgtgccgt acccgctcgc
      901 cttggggtac actcgaaagc ctgcggcgag ttcctgggaa gacctcagtg atctgcgctg
      961 cggtgcggcc gaacggtcgc cagcaaaagt cgggcaaccc ccgactcagt tgaaagcagg
     1021 aaggaaatca atgaagagaa tgagcaatcg tccgcgatgg ctcctcctgt tgttcgtctt
     1081 cgcactggtc gtagcggcct gtgccggaga caccgagagc accacgacaa cggctgctcc
     1141 ggcggagaca acaactacgg ccgcgcaggc gaccacctct acggcggcgg cgccgtcttc
     1201 ggcgatgacc atcacctacg acatcagccc tgatgccgta tggagcgatg gcacaccgat
     1261 tacgactgcc gactttgcct gtacgtggac agcgacgctc aacacacctg ggtcactttc
     1321 gaccgtcgga tatgaccaga tcacttcagt ctcagaaggc tcgagtgccc agcaggtcgt
     1381 cgttgagttc gcaaccgtat acgcccccta caagaacctg ttcaacccgc tgatcccagc
     1441 gcatgtggtc accgacccga catgtatgga cgtgtccgct gacttcttgg acggctacgg
     1501 ggtgtcgggt cgcgcatggc aaatggagtc ctggagcttg gatcaggcga tcttgacgcc
     1561 caacccggcc tactggggcg acgacaagcc agtcgcaacc cgtctggtgc aggtcccgag
     1621 agcagaagat ggcggcatcg cggcgcttca agccggtgaa gtcgacttca tcttcccgca
     1681 ggctttcgcc ggcatcaccg atgccctcaa cgcagacaac atcaagttcg tgcctggtta
     1741 tggcaccaac tacgagggtc tgtatttcca gcagggcgaa gtgcggaatg gtccgtttgc
     1801 cgacgacgac ttccgggcag cgttctcgat gtccatcgac cgtgcgacga tcctggccaa
     1861 catctacgac ccgatcttcc ccggcgcccc actgcttcag tgtggtctct gggtgccgac
     1921 cgtgggcccg tggtgccaga acgatcagtt caccgacagc tatgaccccg agggcgcagc
     1981 tgcgctgctc gagggtgctg gttgggtgaa ggacggcagt ggcatgtggg cgaaggatgg
     2041 tgtcgttccc gaagtcaagt ggatgatcaa caccccgaac ccgcgtcgtg aggccaccca
     2101 ggctctcatg attccgttgc tcgccgaagc cggtttcaac gttgtcgctg acaacggtga
     2161 cgccgcggcc gtgttccagc agcgtttgcc tgccggtgac tacgacctcg ggatgtacat
     2221 caacacagcg tctccggacc cgagcgtgac aggcatcatg tcctgtgacg cggtcccgag
     2281 cgcggacaat ggaaaccagg gtcagaactc agtgttctgg tgcaatgagg acgcaagtgc
     2341 cgtcatggcc gagtccgaca ccacgatcga cgagaccgcc cgtgccgacc tgattcatca
     2401 gatcggtcag gccctcgtgg atgatcacgt gatgttgccc ctgttccagt tcccgaacgt
     2461 cgccgcatgg cggaccgaca ggctggaagg cgatgcccct ggggcagatg cctccaacta
     2521 ccgggcgtac aacaacagct ctcagctgtg gacccctctc gccagtgacg agatcatcgt
     2581 cggcgccgag cagtggcctg agtgcttgaa cccgatcacg gaatgcgcca actcatcgtg
     2641 gatggtctgg accgctgcgt tccccgtgct ccctggtgtc tatgacacca cagccgatgg
     2701 aaacttcgtg gtgaccaacc tgatggcaag cgatcccgta gttacggtca acgagtaacc
     2761 aactagcttg tttggggtgc ccgcgagagc gggcacccca aacccgtagt cataccaatc
     2821 ggagggacga ggaagatgac aaggttcatc gcccttaggg ccatgtgggc catccccgtg
     2881 ctcctcatcg tcacatttct gacgttctgg gcggtcaagt cggcgacaga tccggtcgcg
     2941 agttacaccc ggatcaacac tcgagccaca cccgagatga tcgaggagta caaggtcaag
     3001 cacggcctcg tcggctccac ccccgagcaa tacgtccgct ggctcaaaaa cttcgtcacc
     3061 ctggagtggg gtaactcgat caagggaaat cggccggtct ggcccgacct caaggcggcc
     3121 atggtcacca gcctgattct tggcctgacc gccagttttg tcggaatcac tgtcggcctg
     3181 ggcatcggca tcttgtcggc cctgcgtcag tattcgaagt tcgacaccgc ggcgacaggc
     3241 gccgcctttg tcgggatctc gatcccaccc tttgtgtccg cggtcctgct tcaagtcttc
     3301 tttgcgatca tgatcacgga atggctggac ctggataagc cgttcttacc ggtggccggg
     3361 gtctaccccc caggccacgt cggattcgat ctcggtttgc ggattcagca catgatcctc
     3421 ccggtcacgg tgatcgccat ccagatcatt gcggtctaca gccgatacat gcgggcctcg
     3481 ctgttggacg tcaaggaggc cgactacatg cgcaccgcca gggccaaggg aatctctgag
     3541 cgcaaagtga tcactcatca tgccctgcgc aatgccatga tccccgtagt gaccgttgcc
     3601 gccatcgaca tcggggcgat catcggcggt ctgatcatca ccgagcgcat cttcgaactt
     3661 cacggcaccg gtgactatct gctcactgca ctcggcaacg gcgacttccc acagatcatg
     3721 ccactgatgt tcatcatcgt gagctcggtc atcgtcttca acttggtagc cgatgttgcc
     3781 tatgcctggc tcgacccgag gatccgcctt ggttgatgtc gcttccccga ggccggacac
     3841 ggttgtcgtg ccggaactgg acgagctcga aggcataccc gtagtttcga aatctccgcg
     3901 acaactcgcc atgcgccgct atcgccatca tcgggcggcg atgatctcca gcgtcatatt
     3961 gctgatcatc accctggcgt gcgttttcgc cacctgggtt gctccctacg gtgagaacga
     4021 ggcaatcaag tccactgatg gtggcctccg caatgaaatc ctggtgccgc ccaactcaca
     4081 gttctggttc gggaccgact ccatcggccg cgatctctac tcacgggtgc tctacggagg
     4141 gcgcgtttcc ctcttcgtcg gtatcgccag cgccatctct gctgggctca tcggcactgc
     4201 ggtcggatct tttgcaggct ttcgaggggg gaggatcgac aacgcgttga tgcgtttcac
     4261 cgatctcttc atagggctcc cgctgttggt gatcctgatc atcatgagac ttcttcctga
     4321 gaagcagccg tgggccgcga cggtgctcgg cgaacccggc tcgaagcggt tgatggtgac
     4381 cttgatcaca cttgtgtcct ggacggccac cgctcgaatc gtccgcggcg tcgtgttgag
     4441 cctgaaagag aaggaattcg tggaagccgc gagggcggtc ggcgccagtg accggcggat
     4501 catcaccaag cacttggttc ccaacaccat cggcccgatc gtggtctcga tgaccttcat
     4561 cgtcgcgggt gccatcgccc tcgaatcgac gctgtccttc ttcggattcg ggctggatcc
     4621 gacccaatct tcgtggggca acctgctcgc cgacgcgggg acctcggtga tcaccggaaa
     4681 gtggtggctg gtcttcttcc ccgccatggt gctgttggtc acaatcctct gcatcaactt
     4741 catgggtgac gggctccgtg atgcgctaga cccgaagcag gagcaaagga ccactgggtg
     4801 acccagtcgg tcctatcggt ccgcgacctc tacgtatcat tccccacaca agagggtttg
     4861 gtgcaagcgg ttcgtggggt cagcttcgac attgcggagc acgagctcct cggggtggtc
     4921 ggagagtcag ggtccggtaa gtcggtcacc ttcctggcgg ttatgggcct tctccccaaa
     4981 tcagccatcg tcaccggctc catccaggtg gggggccagg agcttgtggg ccggccggcg
     5041 gacgaggttc gcaaagtgcg cggcaagcaa gtggcgatga tcttccagga tccgctgacg
     5101 gctctcaacc cggttcatcg ggttggtgcc caggttgtcg agatgatcct cgcccatcaa
     5161 gacatctcag atgacgaggc ggattccaag gcggtggagc tcctggacct ggttgggatc
     5221 ccacagcctg atgtgcgagc gcggcagttc ccccacgagt tctccggggg gatgcgccag
     5281 agggcggtga tcgcaatggc gatcgcaaac tcgccggaga tcctgatcgc cgacgagccg
     5341 acaaccgccc tcgacacgac agttcaggct caaatccttg atgtgatcca gaacgtgcaa
     5401 cacgagttgg gtgccgcggt ggccttcatc acccacgacc ttggtgtcgt cgcgcgtatc
     5461 gccgaccggg ttcaagtgat gtatgcgggt cgggtcaccg agcgcggcga ggcgcacgac
     5521 atcttcctcc gcccaactca tccttacacc cgggggctac tcacctctct gccggatgcg
     5581 aacatcgagc ggctcgttcc catcccgggc gccccaccga acatgatgcg gcccccgagc
     5641 gggtgcgcct tccgcatccg ttgcgcttac gccatagatg catgctccgg cgagattccc
     5701 gacctcaggc ccttcgggtc gattgaaact gcatgcatcc gggtcgaaga gattgccgcg
     5761 gaggtgagtg cttgagcgag ccactgctcg aggtaaccga tctcttcaag ctcttcgagg
     5821 tgcgtcagaa tgtcggagcc aaagtcatca agcagacggt ccacgcagta tcaggtgtct
     5881 ctttcagtct gcaaccgggt gagaccctcg ggctcgtagg tgagtccggg tcgggcaagt
     5941 cgacggtggg tcgatgcatc ctccgcctgc tcgagccgac ctccgggtcg gttaggttcc
     6001 gcggccagga gatgaccacg ctcagtcgtg aggagctgcg cgggctccgc tccgagatgc
     6061 agatcgtctt ccaggaccca tatgcctcgc tcgaccccag aatgaccatc ggcgcggcaa
     6121 tcgcggagcc cttggaagtg cacaaggtcc cgggagacca tcgggcaatc gttggcgagt
     6181 tgctcgaact ggttgggctt tcgccagatc acgcgacgag atttccccac gagttctctg
     6241 gtgggcaaag gcagcgaatc gggatcgcca gggccctcag cctgcgccca tctctgatca
     6301 tcctcgacga gccggtctcg gctctcgacg tgtcgattca ggcaggcatc ctcaatttgc
     6361 tcgaggatct tcagacggag ctcagtctgt cgtacctctt catcgcccat gacttgtccg
     6421 tggtgaggca tatcagcgat cgaatcgcgg tgatgtacct gggcaagctc atggaggttg
     6481 gcgaccacaa ggaggtgtac gaccgctcga tacatcccta cacccagtcc ctgctttcgg
     6541 cggtcccgat ccctgatccc acgatcgagc gcacccggca tcggatcgtc ctcgccggtg
     6601 atctgcccag tcccgtcaac ccgccgtcgg gttgcgtctt ccgcacccgg tgcccgatcg
     6661 cattcgacct ctgtgccgag gaagttcccg agttgcgggc gcattcgaag gatcacctcg
     6721 ccgcctgcca cgccccgctt ctcccaggcc agtctctcgc cgatgtggtg gcgtcgcgga
     6781 ccccggtcgc caccaccgtc gtcgacgagt agtcagcggc tgtagaagcg gtactcggtc
     6841 gtttggcggt aggtgccgcc gggctcgaga agcaccgtcg ggtactgcgg ttggttgggt
     6901 gagtccggga aatgctgtgc ttccaagcag attccgcccc gtgtcggata gaccaccccg
     6961 tcctttccag tcgtcggcgg catatgaccg cccgagtaga cctggactcc gggctcggtg
     7021 gtgtgcagct ccatggcgag cccgttcggc ggactctcca gggtggcggc gaggcgcagc
     7081 gtcccgtccc actccgagag ca
//
LOCUS       DBNHXU010000037         5079 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1599798, whole genome shotgun sequence.
ACCESSION   DBNHXU010000037 DBNHXU010000000
VERSION     DBNHXU010000037.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5079)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5079)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5079
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1599798"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1168)
                     /locus_tag="AC161X_01040"
     CDS             complement(<1..1168)
                     /locus_tag="AC161X_01040"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF002367.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988163.1"
                     /translation="MVFRAITPDEYPDLVRTMGLAFSFDPPADDHFRHVLPLERTVIG
                     FDGSKMVSSSAAFSLDMTVPGGTINCGGTTIVSVAPTHRRRGVLNAMMRAHLDEVLSR
                     GEPIAALWASDSAIYGRYGFGQASVHYDVKVRRDHVGFNRLAPEPAPVRLVDADQVGD
                     LLMPFYDEMRKSTPGFYARSPVWWEHHSMADPEPRRGGATAYRFGVVDGAEGIVGYVQ
                     YRAKSDWDDSHGAGTITVRDLFGTTPESWAGLWSYVLNHDLVAEVTAQRRPVDDPLFD
                     FLAGPRRVKAQRFDNLWVRIMDVPRALEGRAYSGPVDVVLEVHDPMGDIEGRYRLVAS
                     PEGAECTRTDAKASITLDAEDLGAVYLGRSRLRAMSRSGRVQGDPASLAAADAAF"
     gene            complement(1174..1776)
                     /locus_tag="AC161X_01045"
     CDS             complement(1174..1776)
                     /locus_tag="AC161X_01045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988164.1"
                     /translation="MAANPDPKPGRWILPLVILGMIAFTYFFVSSLPEGSPTTTLALG
                     STTTTLEGTGTTQPGTATTVPNAAQPYLDELDSINGELQLLSTEMVTVNEGFDADPRT
                     VEFDDAGTRLEVVVTDTQALADRLAALTPPAGLETNHEALISALDFCAGAAQDALDGL
                     RSTDTGELRNAAVDVYTTSAAGFDTEVQNAKNAAGATPDA"
     gene            1884..2705
                     /locus_tag="AC161X_01050"
     CDS             1884..2705
                     /locus_tag="AC161X_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988165.1"
                     /translation="MESDRNEVYERIPWETLERKGGDRQWWMIAVAGAIVLGALAYSF
                     MSNRPVAVPTQPIQATASTTAEAPAATVAAPPIANPSSPTVVAEADLYAVDPERLIDQ
                     ATAHAEWFVAEYIGVDGSEESQATLAALLPAGISPPVAPETTRVFVEWVRAVSLEEVA
                     PLHYRVGVLVRSLVAQGEEAYRRVAPMLATVEILVAEDGARVVMPPTVSPLPTPQPEP
                     LALVEVPPEIQQAAMTSSGATEVLGGVQTAAGDWQVVVMAPGLDGVTRPTTVNVP"
     gene            complement(2706..2963)
                     /locus_tag="AC161X_01055"
     CDS             complement(2706..2963)
                     /locus_tag="AC161X_01055"
                     /inference="COORDINATES: protein motif:HMM:NF020295.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU N-terminal domain-containing protein"
                     /protein_id="HIR4988166.1"
                     /translation="MPVSVAPTPNPNAAKFSVGQPVGGPATFVAGSNPDDPLAAGLLG
                     ITGVTSIFMTADFVTISKAPDSSWESIIPEATAILETRFSG"
     gene            2984..4882
                     /locus_tag="AC161X_01060"
     CDS             2984..4882
                     /locus_tag="AC161X_01060"
                     /inference="COORDINATES: protein motif:HMM:NF014071.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0017168 - 5-oxoprolinase (ATP-hydrolyzing)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HIR4988167.1"
                     /translation="MAVTVGVDVGGTFTDIVALDGETLIGGKVPTTPDQSVGVIGALS
                     DLVAEPIALFLHGTTAGTNALLEERGARVALVTSEGFEDLVEIGRQARPSLYDPFVDR
                     PLPLVSRMARIGARDKDQTLGKLVELQPEAVAVALVRSYADSSMEVELASAIEARLGV
                     PVSTGAGVSPGFREYERIATTVLNAYLSPEMSGYLQRLGEGILTPGRLVMTSAGGLLP
                     FESAAELPGRLVLSGPAAGVVAAAELARAKGHRSAISFDMGGTSTDVCRITDGEVVAG
                     MGRKVAERVNRVPSLPVNTIGAGGGSIGWRDDGGALRVGPISAGAVPGPVAYGRGGML
                     PTVTDANVVLGFLPPGLALAGSVPLDVVAAAQALALLGREVGLEAEATARGMIEIVDS
                     HMERAIRTVSVEEGSDPREAALIAFGGAGGLHAARLAKRLGMRTVLVPPLSGVFSALG
                     LLLARPRTDAIRTVLLEEGSDSLGQAIDQVVAQARSTFDAMEVSGPTEVVVLGAVRYV
                     GQSHELTVFADPDWGGLRQRFEESHRSHFGFDRPEEPIELVEVRAEMVGDPSLTWSDL
                     PAKSARGIPEPVSTTDQGFVVWDRDHLPSGFEIEGSALIVERDSVTLLEPSDRLTVHD
                     DGTLEISL"
BASE COUNT          850 a         1603 c         1738 g          888 t
ORIGIN      
        1 tgaaggcggc gtcggcggcg gcgagggagg cgggatcacc ctgaaccctg ccgcttcgtg
       61 acattgccct cagtcgtgac cgaccgaggt agacggcccc caggtcctcg gcgtcgaggg
      121 tgatcgaagc cttggcatcc gttcgcgtgc actcggcccc ttcgggcgag gccacaagcc
      181 ggtaccggcc ctcgatatcc cccattgggt cgtggacctc cagaacgacg tccacagggc
      241 ccgagtaggc gcgaccttcg agggctcgcg gaacgtccat gatccgcacc cagagattgt
      301 cgaacctctg ggctttgacc cgtcggggcc cggcgaggaa atcgaacagc gggtcatcga
      361 ccggccgacg ttgggcggtg acttcggcca ccaaatcgtg gttgaggacg tagctccaca
      421 gacccgccca cgactccgga gtagtgccga agaggtccct cacggtgatc gtccccgcac
      481 cgtggctgtc gtcccaatcg ctcttggccc gatactggac ataaccgacg atgccttcgg
      541 ccccgtcgac taccccgaaa cggtaggcgg tcgctccacc ccgcctcggt tccgggtcgg
      601 ccatcgagtg gtgctcccac cacaccggcg accgggcgta gaaaccgggg gtggacttac
      661 gcatctcgtc gtagaacggc atgagcaagt ctccaacctg gtcagcatcc accaggcgca
      721 ccggggcagg ttccggcgcc aggcggttga acccgacgtg atccctccgc accttgacgt
      781 cgtagtggac cgacgcctga ccgaacccgt atctgccgta gatggcgctg tcagatgccc
      841 agagcgcggc gatcggctca ccccggctca gcacctcgtc gaggtgggcg cgcatcatgg
      901 cgttgagtac gccgcgtcgg cgatgggtgg gggccaccga gacgatcgtc gtgcccccac
      961 aattgatggt cccgccgggg actgtcatgt cgagggagaa ggcggcagag ctgctcacca
     1021 tcttcgagcc gtcgaagccg atcactgttc gctccagggg gaggacgtga cggaagtggt
     1081 cgtcagccgg cgggtcgaag ctgaaagcaa gacccatcgt ccgaaccaga tcggggtact
     1141 cgtcgggcgt gatagcccgg aaaaccaccg ccatcaggcg tcgggcgtgg cgcccgcggc
     1201 gttcttggcg ttctggactt cggtgtcgaa gccggcagca gaggtggtgt agacgtcgac
     1261 cgccgcattc ctcagctcac cggtgtcggt ggagcggaga ccgtcgagtg catcctgagc
     1321 ggccccggcg cagaaatcaa gggccgagat cagagcctcg tgattggtct ccagccctgc
     1381 aggcggggtc agcgccgcca accgatcggc aagtgcttgg gtgtcggtca caaccacttc
     1441 gagccgggtg ccggcgtcgt cgaactcgac ggttctcggg tcggcgtcga aaccttcgtt
     1501 cacggtcacc atctcggtgg acaacagctg cagctcgccg ttgatggagt ccaactcgtc
     1561 cagatagggc tgtgcagcgt tggggaccgt ggttgcggtg ccaggctggg tcgtcccggt
     1621 tccttccagc gtcgtcgttg tgctccccag tgccagggtc gtcgtcggcg agccttcggg
     1681 gagggaactg acgaagaaat aggtgaaagc gatcatcccg aggatcacca gcgggaggat
     1741 ccagcggccc ggtttcgggt cggggttggc tgccataact ggcgaaacga tagctccaag
     1801 ccccctggtt cccgggttga cccgcatcgc ctcccaacac gaccctcttg aaccgccctt
     1861 ggggcctcca taggttcggc gacatggaga gcgacagaaa cgaggtctat gagcggatcc
     1921 catgggagac gctcgagcga aaaggtggcg atcggcagtg gtggatgatc gccgtggcag
     1981 gggccattgt gcttggcgca ctcgcctatt cattcatgag caaccggccg gtggcagtgc
     2041 caacccagcc gatccaggcg actgcgtcca ccacagctga ggcaccggcg gcgaccgtgg
     2101 cggcgccacc gatcgccaat ccgagcagcc cgacagtggt ggctgaggcc gatctctacg
     2161 cggtcgatcc cgagcgtctc atcgaccagg cgaccgccca cgccgagtgg ttcgtcgctg
     2221 agtacatcgg cgtcgacggc tcggaggaga gtcaggccac cttggccgcg ctgctcccgg
     2281 cggggatatc tcctcccgta gcaccggaga cgacaagggt gttcgtggaa tgggtgcgcg
     2341 cggtctcgtt ggaagaggta gcgccgctgc actatcgagt gggggtgctg gtccgttccc
     2401 tggtggcgca aggcgaggag gcctatcgcc gggtggctcc gatgctggcg acggtcgaaa
     2461 tcctcgttgc ggaggatggc gcacgggtgg tgatgccacc gactgtgtcc ccgctgccaa
     2521 cccctcaacc ggagccgttg gccctcgtcg aagttcctcc cgagattcag caggcggcga
     2581 tgacctcgtc gggagcgacc gaagtcctcg gtggtgtcca aacagcggca ggcgactggc
     2641 aggtggtggt gatggcccca ggcctcgacg gtgtcacccg ccccaccacg gtcaacgtgc
     2701 cctgatcaac cgctgaatcg ggtctctagg atcgccgtcg cctcagggat gatcgattcc
     2761 caggaggagt cgggagcctt ggagatggtt acgaagtcgg cggtcatgaa gatgctggtg
     2821 acccccgtta tgcccagcag tccggcagcc agggggtcat ccgggttgga acccgcgacg
     2881 aaggtggcgg ggccaccaac cggctgcccg acggagaact tggcggcgtt ggggtttggg
     2941 gtgggggcga cgctcaccgg cataggtcac cgaggctaat cgggtggcgg tcaccgtcgg
     3001 tgtggatgtc gggggcacct tcaccgacat cgttgcctta gacggggaga cattgatcgg
     3061 gggcaaggtg ccgaccactc ccgatcagtc ggtgggggtc ataggggccc tttccgatct
     3121 cgtcgccgaa ccgatagcgc tgtttctcca tgggaccacc gcgggcacca acgccttgct
     3181 ggaggagaga ggagctcggg tcgcactggt cacgtctgaa ggattcgaag acctggtcga
     3241 gatcgggcgc caggcgcgtc cctcgctcta cgaccccttc gtcgaccgtc ccctccctct
     3301 ggtctcacgc atggcacgga tcggagctcg agacaaggac cagaccttgg ggaagctggt
     3361 cgaactccaa ccggaagcgg tggccgtcgc cctggtccgg tcctacgccg actcgtcgat
     3421 ggaggtcgag ctggcgtctg ccatcgaggc ccggctcggc gtcccggtgt ccacaggtgc
     3481 tggcgtatcg cccggattcc gggagtacga gcggatcgcg accaccgtcc tcaacgccta
     3541 tctatcaccc gagatgtcgg gataccttca gcgtctcgga gaagggatcc tgacgccggg
     3601 ccggctggtg atgacctcgg ccggtgggct gctcccgttc gagtctgcgg cggaactccc
     3661 cgggcgactg gtgctctccg gtcccgcagc cggggtggtc gctgcagccg agctggcgcg
     3721 agccaaaggc catcgctcgg cgatcagctt cgatatgggc ggcacctcca ccgacgtgtg
     3781 ccggatcacc gacggggagg ttgtcgccgg catggggcga aaggtcgcgg agagggtgaa
     3841 tcgagtgcct tcgctcccgg tcaacaccat cggggcgggc ggaggaagta tcggctggcg
     3901 cgacgatggc ggtgccctca gggtggggcc tatctccgcg ggggcggtgc cggggccggt
     3961 ggcctacgga aggggcggca tgctgcccac cgtcaccgac gccaacgtcg tgctcggctt
     4021 tctgccccca ggcttggctt tggcgggctc ggtgccactc gatgtggtcg cggcagcaca
     4081 ggctctggct ctcttgggtc gagaggtagg gctggaggca gaggcaacgg cccgggggat
     4141 gatcgaaatt gtcgactccc acatggagcg agccatcaga acggtttctg tcgaggaggg
     4201 gtcggaccct cgcgaggctg ccctcatcgc tttcgggggc gccggtggcc tccacgccgc
     4261 ccggctcgcg aaacggttgg ggatgcgcac tgtcctggtc ccaccactct ctggtgtgtt
     4321 ctcagctctc gggcttctcc tcgcgaggcc acggacggat gcaatccgca ccgtgcttct
     4381 cgaggaagga tcggattcgc tcggacaggc aatcgatcag gtcgtggcac aggcccgatc
     4441 gaccttcgat gcgatggagg tgtcggggcc gacggaggtc gtcgtcctcg gagcggttcg
     4501 atacgtgggc cagtcccatg agctcaccgt cttcgccgac cccgactggg gcggtctccg
     4561 acaaaggttc gaagagagcc accgatcgca cttcggtttc gaccggccag aggagccgat
     4621 cgagcttgtt gaagtccgcg ccgagatggt gggtgatccg tctctgacat ggagcgacct
     4681 cccggccaag tcggcccgtg gaattccgga gccggtctcg accaccgacc aaggattcgt
     4741 ggtctgggat cgcgaccacc tcccttccgg attcgagatc gaggggtcgg cgctcatcgt
     4801 ggaacgggac tcggtaaccc ttctggaacc atcggatcgt ctgacggtcc acgacgacgg
     4861 gaccttggag atcagcctgt gagcgaattg tccgcccaaa cacctgccag cgaccgagag
     4921 tagggtcgcc ggtccatacg gaacgccccc aactggcgtc ctagccgcga aacgaaggag
     4981 gaccgatatg acttctcgct ctcgtcggtg gtggagtccc gccatcgtgt tactcagcgt
     5041 tctggctctc gccttgagca gccttccggc acttgccac
//
LOCUS       DBNHXU010000038         4590 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2195785, whole genome shotgun sequence.
ACCESSION   DBNHXU010000038 DBNHXU010000000
VERSION     DBNHXU010000038.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4590)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4590)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4590
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2195785"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1062
                     /locus_tag="AC161X_01065"
     CDS             <1..1062
                     /locus_tag="AC161X_01065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020462159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HIR4988168.1"
                     /translation="AEDELTLAANTDAYRRVTFRPRVLRDVGEIDTSSTLLGKPISMP
                     LVLAPTGFTRIADPQGELAVARAAERAKIPYALSTLGTRSIEELAATGASRLWFQLYV
                     WKDRGLVSDLVQRAAAAGYEALCLTVDAQWFGRRERDARRGFTLPPKLGPSTLFDGIV
                     HPGWTWQFLTSEPIIFANVVGREVGAGEEAITLAEYVAQQFDRALSWKDVEWLRGIWN
                     GPIVIKGIQTVADAVIAADAGVEAIALSNHGGRQLDSAPTPIDLLPEVAAAVSGRTEI
                     ICDGGVRRGSDIVKALALGANACMAGRAYMYGLGAAGEEGVDHVLNLLSTDLKRTMAL
                     VGCKTIGDIGPEYVSLEGR"
     gene            complement(1064..1954)
                     /locus_tag="AC161X_01070"
     CDS             complement(1064..1954)
                     /locus_tag="AC161X_01070"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR4988169.1"
                     /translation="MRDRPTVGFIGLGRMGSLMAANVARAGFPMRIYNRTQAVAEEFA
                     TEHGGSAMPTLRELAEGCDFVVSMLADGDALVEAYTDPGGVLEGLAAGSVAIDMGTSG
                     TEAVQQVRDRVEAAGSSFVDAPVSGSTPAAREAQLLIMVGGTDSQFEQVRPVLEAMGT
                     PVQVGPSGSAAALKLTVNSILYGLNEAVAEGVVLAERAGVKPEISLDILTRSAAGAPL
                     VKYRTPQYLNPDEAPVMFTLDLARKDIRLALDQARELGVPMPQLERTLEVVEQLIADG
                     EGARDMGFVVEGARRRAQGR"
     gene            complement(1980..2438)
                     /locus_tag="AC161X_01075"
     CDS             complement(1980..2438)
                     /locus_tag="AC161X_01075"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR4988170.1"
                     /translation="MSIGFEGVVEMQDGRATKVHLDEITYADGLKPGEGWIDMKVQFL
                     ITEENAGAQEVVFGRTVFTPGSRHESHRHQNAEEIQYLVAGEGIVLDGDDEIPMKVGD
                     VIHTKKGQGHGFRNTSASEDAVLIWLWAGAGSRDAAGYEARSPVADGTKD"
     gene            2542..3633
                     /locus_tag="AC161X_01080"
     CDS             2542..3633
                     /locus_tag="AC161X_01080"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR4988171.1"
                     /translation="MRAAVYHGPNDVRFEQVEDPVPGPGELLIDVATVGICGTDVAEY
                     VNEPHFFPVTERHPHSGHLGPTIPGHEFSGFVSGVGAGVEGFSIGDLVASGAGVACGV
                     CPPCVRGATNLCTSYWTVGLHTHGAMAEKVVVPASCCLNVADSNIAPDLAALAQPMSI
                     ALHAVRRGRLSADDLVLIVGVGGIGSFLTYCASAQGAEVIAVDLDQGRLDMAMALGAT
                     RALLVSASDEPGDRLIDGDRQPTLAFECTAAPGPLKMAIRVVADNGRVVVVGHQPRPI
                     EFDFKVLTFGEKEMIGTMAHAYAADFNDAVDLVNRRPDVWSHLAPTVHPLEDLVTAGL
                     LPMAEHRQNQVKTLFDPSLREPRPIHTRT"
     gene            complement(3634..4365)
                     /locus_tag="AC161X_01085"
     CDS             complement(3634..4365)
                     /locus_tag="AC161X_01085"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4988172.1"
                     /translation="MDGGYYRSDLSAVHHKGFGFHADMCAPGILHHLEPVLERGGLVV
                     ELGCGSGRLTRYLVDAGHRVIATDASPAMLDLAREYVPEADLRQLVLPDDPIPMADAI
                     VSTGHPLNYLADEGEVDRALVACAMALRPGGVLAVDICDLEWGRARMDAPPSARVGDD
                     WAIITMFSVPAPNQFDRQIASFLRNDDGSWRRDDETHHNVLVDTTLIPTFLADYAVTA
                     EVLPAFGSEQFPAGLRAVVGKKQSD"
     gene            complement(4383..>4590)
                     /locus_tag="AC161X_01090"
     CDS             complement(4383..>4590)
                     /locus_tag="AC161X_01090"
                     /inference="COORDINATES: protein motif:HMM:NF021604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016989 - sigma factor antagonist activity
                     [Evidence IEA];
                     GO_process: GO:0006417 - regulation of translation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR4988173.1"
                     /translation="LEPLGADRTYQLWVITPDEVAISAGVLGNEPGPSRFTWTADVAG
                     FALTREVAGGVVSSAGDVVSVANL"
BASE COUNT          835 a         1488 c         1418 g          849 t
ORIGIN      
        1 gccgaggacg aactgaccct cgccgccaac accgatgcct accgccgcgt gaccttcaga
       61 ccacgggtcc tccgtgacgt cggtgagatc gacacctctt cgacactcct cggaaagcca
      121 atctcgatgc cgctggttct cgctcccacc gggttcacca ggatcgccga tccccaaggt
      181 gaactggccg tggcccgagc tgcggagcga gccaagattc cctacgcgct gtcgacattg
      241 gggaccagat caatcgaaga gctcgcggca accggagctt cacgcctctg gttccagctc
      301 tatgtatgga aggaccgcgg gctggtgtcc gatctcgtac aacgggccgc tgcggccggc
      361 tacgaggcgc tatgcctcac cgtcgatgct cagtggtttg gcagacgtga gcgcgacgcc
      421 cggcgaggct tcaccctccc gcccaaactc ggcccttcca ccctcttcga cggaatcgtc
      481 catccgggct ggacctggca attcctgacc tcagagccaa tcatctttgc aaacgtggtt
      541 ggccgtgagg ttggagccgg ggaggaggcg atcacgctcg ccgagtacgt ggcacagcag
      601 ttcgaccgcg cgctgtcctg gaaggacgtc gaatggctcc gcgggatctg gaacgggccg
      661 atcgtgatca aggggatcca gaccgtggcc gacgccgtca tcgcggccga cgcaggggtt
      721 gaagcgatcg cgctgtctaa tcatggcggt aggcagctcg actcggcccc cacgcccatc
      781 gacttgctcc ctgaagttgc cgcggcggtc agtggtcgca ccgagatcat ctgtgatggc
      841 ggagtgagaa ggggcagcga catcgtcaag gccctggccc tgggcgcgaa tgcctgcatg
      901 gccggtcgtg cctacatgta cggactcggg gccgccggtg aagaaggcgt ggatcacgtc
      961 ctcaacctgt tgtcgacaga cctgaaacga acgatggcac tggtcgggtg caagaccatc
     1021 ggagacatcg ggcccgaata cgtgtctctc gaaggtcgct gagttatcgg ccctgcgctc
     1081 ggcggcgggc cccttcaacg acgaaaccca tatcccgcgc cccttcgccg tcggcgatta
     1141 gctgttcgac gacctcgagg gttcgctcca gttgaggcat cggcacaccc aactccctgg
     1201 cttgatcgag cgctaggcgt atgtccttgc gagcaaggtc gagggtgaac atcactggcg
     1261 cctcatctgg attgaggtat tgcggtgtcc ggtacttgac caacggtgcc ccagccgcgc
     1321 tccgagtcaa tatgtccagt gagatctcgg gcttgacacc ggcccgctcc gcgaggacga
     1381 caccttcggc caccgcctca ttgagcccgt agagaatcga gttgaccgtc agcttcaaag
     1441 cggcggccga gccggaaggt ccgacctgca cgggcgttcc catggcttcg agcacgggcc
     1501 tcacctgctc gaactgggaa tcggtgccac ccaccatgat caagagttgc gcctccctcg
     1561 cggctggcgt gcttcctgac accggggcat cgacgaatga cgatccggct gcctcaactc
     1621 ggtcccgcac ctgctgcacc gcttcggtcc cactggtgcc catgtcaatg gcaaccgacc
     1681 cagctgccaa accttcgaga accccaccgg gatcggtgta ggcctcgacg agcgcgtcgc
     1741 cgtcggcaag cattgacacc acaaagtcgc agccttcggc caactccctg agagtcggca
     1801 tcgccgatcc accgtgctcg gttgcgaact cttcggcgac agcttgagtg cggttgtaaa
     1861 tccgcatcgg gaatccagcc cgggcgacgt tggcagccat caggctgccc atccgaccca
     1921 gcccgatgaa tccgacggtc ggtcgatccc tcactacatc atcggaggtc attgtctcgt
     1981 tagtccttag tgccgtccgc gaccggtgac cgggcctcgt agccggccgc gtcacgagag
     2041 ccggcgccgg cccacaacca aatgaggacc gcatcctcgg acgcggaggt gttgcggaat
     2101 ccgtgcccct ggcccttctt ggtgtggatc acatcgccaa ccttcatcgg gatctcgtcg
     2161 tctccatcga gcacgatccc ctcaccagcc accaggtact ggatctcctc cgcgttctga
     2221 tggcggtgcg actcgtgtcg ggatcctggg gtgaacaccg tgcgaccgaa cacgacctcc
     2281 tgagccccgg cattctcctc ggtgatgagg aactggacct tcatgtcgat ccatccctcg
     2341 cctggcttca gaccgtcggc ataggtgatc tcgtcgagat gaacctttgt cgctcgacca
     2401 tcctgcattt ccaccactcc ctcgaagccg attgacaatg ccggccttgg cgggattaaa
     2461 ccagagcgat cgatttctcc tcgaaacgtc gaccatcggg agcggccgac gccccgacta
     2521 gcgtcgcctc atggtgccat attgagggca gcggtttacc acggtcccaa cgatgttcga
     2581 ttcgagcagg tggaggatcc agtccccggc cccggtgagt tgctaatcga tgtggccacg
     2641 gtggggatct gcggcacgga tgtggccgag tacgtcaacg aaccgcactt ctttccagtc
     2701 accgagcgtc atcctcacag cgggcacctc gggccgacca tccccggcca cgagttctcg
     2761 gggttcgtct caggggtggg tgccggtgtc gagggattct cgattggcga tctggtggcg
     2821 tcgggcgccg gggtcgcctg tggggtgtgt cccccttgtg tccgaggcgc aaccaacttg
     2881 tgcacttcgt actggacggt cggtctccat actcatggag ccatggccga gaaggttgtg
     2941 gtgcctgcat cctgttgcct caatgtcgcc gactccaaca tcgcgccgga tctggcggct
     3001 ctagcccagc ccatgtcgat tgcgcttcat gcggtgcgtc gcggccgcct cagcgcggac
     3061 gacctggttc tgatcgtcgg agtgggcgga atcgggagct tcctcaccta ttgcgcctcc
     3121 gctcagggag ccgaggtcat cgcagtcgat ctcgatcagg gccgtctcga catggcgatg
     3181 gcccttggag ccacaagagc attgctggtg tcggcgtccg atgagccggg cgaccgattg
     3241 attgacggcg acaggcagcc gacactcgcc ttcgagtgca cagccgctcc ggggcctttg
     3301 aagatggcga tcagggtcgt cgccgataac ggcagggtgg tggtcgttgg tcaccagcct
     3361 cgcccgatcg aattcgactt caaagtgctc acgttcgggg agaaggaaat gatcgggacc
     3421 atggcccacg cctatgccgc ggacttcaac gacgcggttg acctggtgaa caggcgtccc
     3481 gacgtgtggt cccatctggc gccgacggtg cacccgctcg aagatctggt gacggcgggg
     3541 ctacttccga tggccgagca tcggcagaac caggtcaaga ccctcttcga cccttcgctt
     3601 cgggagccgc gaccgattca tacgcggacc tagtcagtcc gactgcttct ttcccacgac
     3661 agcccgaaga cccgccggga attgctcgct accgaaggcg ggaaggactt cggcggtcac
     3721 ggcgtagtca gctaggaagg tcgggataag cgtggtgtcg acgaggacgt tgtgatgggt
     3781 ctcatcgtcc cggcgccagg agccgtcgtc attgcggagg aaggaagcga tctggcgatc
     3841 gaactgattg ggggcgggga ccgagaacat cgtgatgatc gcccaatcgt cgccgacccg
     3901 ggcgctgggt ggggcgtcca tcctggcacg gccccactcg aggtcacaga tatcgacggc
     3961 aagcacgccg ccgggccgca aagccattgc gcatgccacc agggcccggt cgacctcacc
     4021 ttcgtcagcc agatagttga gtgggtggcc ggtggagacg attgcatcag ccatcgggat
     4081 cggatcatcc ggcaagacca actgtcgaag gtcggcctcg ggcacatact cacgagcgag
     4141 gtcgagcatc gccggggagg cgtcggtcgc aataactcga tgcccggcat cgaccaggta
     4201 cctggtgaga cgaccgctcc cgcaacccaa ctccacgacc aacccgcccc gttccaggac
     4261 aggctcgagg tgatgaagga tccccggggc acacatgtcg gcgtggaacc cgaagccctt
     4321 gtgatgcacc gctgagaggt cagacctgta atagccgccg tccaccgatc agatccttct
     4381 gtttataggt tggctaccga gaccacgtcc ccggcgctgc tgacgacacc gcctgccacc
     4441 tctcgggtga gggcgaagcc ggcgacgtcg gcagtccagg tgaaacgtga gggacccggc
     4501 tcatttccaa gcactccggc tgagatcgct acttcatctg gggttatcac ccacaactga
     4561 taagtgcggt cggcacccaa aggctccagc
//
LOCUS       DBNHXU010000039         4494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_211471, whole genome shotgun sequence.
ACCESSION   DBNHXU010000039 DBNHXU010000000
VERSION     DBNHXU010000039.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4494
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_211471"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC161X_01095"
     CDS             <1..459
                     /locus_tag="AC161X_01095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen
                     decarboxylase/cobalamine-independent methonine synthase
                     family protein"
                     /protein_id="HIR4988174.1"
                     /translation="GVINRSLVNVPRDRVRLHVCWGNSESPHDSDVPLADILPIILKA
                     HVGGLVLPFANPRHAHEYKSFADHRLADDQILVAGVIDTLTNFVEHPEVVADRLERVA
                     SVVGDPTRVLAGTDCGFDTSAGWGRVAEDVVWAKLRSMRDGAELATERLY"
     gene            591..1721
                     /locus_tag="AC161X_01100"
     CDS             591..1721
                     /locus_tag="AC161X_01100"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR4988175.1"
                     /translation="MNEGPLQIGYGLIGCGWVGSAHAWGVAASSGETARLIATSDVDL
                     ALAQQMADRFEAQSAYQDYRELLRRDDIGAVSVCVPDFLHRQVVIDAALAGKHVLCEK
                     PLAMSVAEADEMIAACESNDVELGMIMNHRYAPDNIRAKDALSRGAVGGQLIGSVVHS
                     SGLTGPLGTSPWRGKANRAAGGVLSTQAIHFLDLLLWFMGPVRSVKALTDTLHWDIQD
                     HEDTAVLALRLESGALATLTTTNGSPIMDDFTGTRIEVHGTRGWLALEGDALKHAEFA
                     DAYEAPAVYLPDVPGEASEIIFGLGHVYEVIDFIQAIRSGTEAPIPARDGRHLMAVIE
                     AAYKSAAEGDEVELSKQAVGYTQPSPPGSLLSIGSHRPLQEH"
     gene            1721..2758
                     /locus_tag="AC161X_01105"
     CDS             1721..2758
                     /locus_tag="AC161X_01105"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR4988176.1"
                     /translation="MGSTMRAMVVTEFGSPPVLTEVPRPTPKEDEILLRVRAAGVCHT
                     DMKIRDGIVPDVDLPLVLGHEIAGEVAEIGAGVTTASVGNRGVPYGYVTCGNCRFCLE
                     GRTSLCEQLVLRYGFGSIGGYSEYVVVPERFFVPIPDSVRFDQAAVATCSIVTPYHAL
                     IKRARLRLGETVVVVGAGGGVGLHAVQLAHRTGGDVIAVDVDATREGAAKAQGASSFV
                     VPHEGRFADEVRELTHGRGADVVIDMVASTATMSDSIEALGLGGRLCLVGYQRGQLLT
                     AVTPDVVFREIEIYGSHWATLTDTVEVIDMIDRGLIDPIVIREFPLHEAPAALELLAV
                     GGGPGRSILTV"
     gene            complement(2799..3230)
                     /locus_tag="AC161X_01110"
     CDS             complement(2799..3230)
                     /locus_tag="AC161X_01110"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR4988177.1"
                     /translation="MVERKAINYPGHAAGLDGRIWSPGLKVGPWIFLSGVTSVDYDAK
                     KTVGIEAQGGLTPPKKDPEAQWRQVLTNIKAVMEEAGGTMADVVMANVFVTDMHYYYD
                     YEWIRKEFFVEPYPVCTAVEVGALVHTEWLLEIEVIGYIED"
     gene            3438..4349
                     /locus_tag="AC161X_01115"
     CDS             3438..4349
                     /locus_tag="AC161X_01115"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008712702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR4988178.1"
                     /translation="MVLPSHGRFEYSPITSRPDFSWPGGQRLAVYIAVCMEHFTYGEG
                     GLGLPYSPGIPHPNTYNWAWREYGNRVGGWRLLDLLQQSGIPPTVLLNTACYEHCPEL
                     VDAYRGAGAEFVGHGQTNSVHPNDLTEDDEREMIRDVFETILRHEGKPPGGWMSPGAN
                     PSAVTEDLLAETGFTYTLDWPMDDQPTWLRTRGGPLLSVPYPHEVNDVPMIVFHHGTA
                     PAYADMMTDNLAEMVEQSQHQPLVCGIVTHTFIMGQPYRLRRFRQAIEYLNGLSGVWL
                     TTPGEIAAHYAGITTPEQALFGSISTT"
BASE COUNT          840 a         1384 c         1400 g          870 t
ORIGIN      
        1 ggggtgatca acaggtcgct ggtcaacgtg ccgcgcgacc gagtccgcct ccatgtgtgt
       61 tggggcaatt cagagtcacc gcacgacagt gacgtcccac tcgccgacat cctgcccatc
      121 atcctcaagg cccatgtcgg tggattggtc ctcccctttg ccaacccacg ccacgcccat
      181 gagtacaagt ctttcgccga ccatcggctt gccgacgacc agatcctggt cgccggggtc
      241 atcgacacgt tgaccaactt cgtcgagcat ccggaagtag tcgctgaccg actcgagcgc
      301 gttgcctcgg tcgtcggcga cccgactcgg gtgcttgccg gcaccgattg tggattcgac
      361 acctcagctg gatggggtcg ggtagccgag gacgtggtct gggccaagct gaggtcaatg
      421 cgtgacggcg ccgagctggc aaccgaaagg ctctactgag ctggtcaccg tttggccccc
      481 ctgcattcta tataacatag gctgtccgtt gtcacgggct cgcaaggcaa agaagggctc
      541 caatccatga actcacccct ggttgtcgac agattcaagg gcacatccca atgaatgagg
      601 gtccgctgca gatcggatat gggctgatcg gttgcggctg ggtcggttct gcccacgcct
      661 ggggcgtggc cgcgagttcg ggcgagacgg cccgcctaat agcaacgtcc gatgtcgatt
      721 tggcgcttgc ccaacaaatg gcagatcgtt tcgaggcgca gagtgcgtat caggactacc
      781 gagaattgct gaggagggac gacatcgggg cggtgtcggt gtgcgtcccc gactttctcc
      841 atcggcaagt agtcatcgac gcagcgctgg caggcaaaca cgtgttgtgt gagaagccac
      901 tggccatgag tgttgccgag gccgacgaga tgattgcggc ctgcgaatcg aacgatgttg
      961 agctcgggat gatcatgaac cacaggtatg ccccggacaa catcagggcc aaggacgcgc
     1021 tatccagggg agccgttggt ggccaactca tcggaagcgt cgtccactcc agtgggctta
     1081 ccggccccct cggcacctct ccctggcggg gcaaggccaa tcgagcggct ggaggcgtgc
     1141 tgagcacaca ggccatccat ttcctggacc tgctgctctg gttcatgggc ccggttcgct
     1201 cggtcaaggc actgaccgac accctgcatt gggacatcca ggaccacgaa gacaccgctg
     1261 tcctggcgct taggttggag tccggagcgt tggcgactct caccaccacc aacggcagcc
     1321 cgatcatgga tgacttcacc ggcacccgga tcgaggtgca cggcacacgt ggatggcttg
     1381 ccctggaagg ggatgccttg aagcatgccg agtttgcaga tgcctacgaa gcgcctgctg
     1441 tgtaccttcc cgatgtgccc ggtgaggcca gcgagatcat cttcggcttg ggacacgtgt
     1501 acgaagtgat cgacttcata caggccattc gctcaggaac cgaggccccg attccggccc
     1561 gagacgggcg tcacctcatg gcagtcatcg aggccgccta caagtcggcc gccgagggcg
     1621 atgaggtgga actgagcaag caggcggtcg gttatacgca gccttccccg ccaggctcgc
     1681 tcctttctat cgggtcccac cgacctttgc aggaacattg atgggatcga ctatgcgtgc
     1741 gatggtggtg accgagttcg gaagtccgcc cgtgttgacc gaggtccccc gtccgacgcc
     1801 gaaggaagat gagattctgc tccgggtccg cgctgccggg gtttgccaca ccgacatgaa
     1861 gatcagggat gggatagtcc ccgatgtcga ccttcccctg gtactcggcc acgagatagc
     1921 gggcgaagtg gcggagatcg gtgccggagt caccaccgcg tcagtaggaa accgaggagt
     1981 tccgtacggg tatgtgacat gtgggaactg tcgcttctgt ctcgagggac ggacatcgtt
     2041 gtgcgagcaa ttagttctcc ggtacggctt cggctcgatc ggcggatact ccgaatacgt
     2101 tgtcgtgccc gagcgcttct ttgttccgat acccgacagc gtcaggttcg atcaggcggc
     2161 cgtcgcgact tgctcgatcg tcacccccta ccacgctctc ataaagcgag cgcgactcag
     2221 attgggcgag acggtggtgg tggttggcgc cggcggaggt gtcggattgc atgccgtgca
     2281 actcgcccac cgaacgggtg gggacgtcat cgcagtggat gttgacgcga cgcgggaggg
     2341 cgccgccaag gcgcagggcg ccagcagctt cgttgttcct catgagggtc gattcgccga
     2401 tgaggtgcgc gaactcacac atggtcgagg tgccgacgtc gtcattgaca tggtggcctc
     2461 aacggcgacc atgtcggatt cgattgaagc cttgggactc ggagggcgtt tgtgtctggt
     2521 cggctaccag cgagggcagc tattgaccgc cgtcacccct gatgtcgtat ttcgcgagat
     2581 cgagatctac ggatcccatt gggcgaccct gaccgacacg gtcgaggtca tcgacatgat
     2641 cgaccggggt ctaatcgatc ccatcgtcat tcgggagttc ccgctgcacg aggcacccgc
     2701 cgcgctcgag ctcctcgccg ttggcggagg accgggtcgc agcattctca cggtctaggt
     2761 cgggcgggct tccggcagcg gcttgaaatg gcgcccgatc agtcctcgat gtagccaatg
     2821 acctcgatct cgaggagcca ctccgtgtga actaaggcgc cgacttcaac agcggtgcag
     2881 accgggtagg gctcgacgaa gaactccttc cggatccact cgtagtcgta gtagtaatgc
     2941 atgtcggtca cgaacacgtt ggccataacg acgtcggcca tggtgccgcc ggcctcttcc
     3001 atgaccgcct tgatgttggt cagaacttga cgccactgcg cctcggggtc ctttttcggc
     3061 ggagtaagcc ccccctgcgc ctcgatcccc acagtcttct tcgcgtcata gtcgacggaa
     3121 gtgactcccg agaggaagat ccatgggccg actttcagac ccggactcca aattcgaccg
     3181 tccaggcccg ccgcgtgccc cgggtagttg atcgccttcc tctccaccat gactcgctcc
     3241 ttctgcgtct caccaccaga tgttgtctgg ccggggcgca acactacgtt cgggccaatc
     3301 ggttgcaaag tcgtcccctc tttgacctct aaccgccgtg ctgtaatgct ccgaaggcga
     3361 cggtgcgcgc cgttgggcca ccgccgtcga gtcacggggt tgctcaaatg cctcgagcga
     3421 gatcgaagga gcgttaggtg gtgttaccca gtcacgggcg attcgagtat tccccgatca
     3481 cttcaagacc tgacttctcc tggccgggag ggcagcgcct cgccgtttac atcgccgtgt
     3541 gcatggagca tttcacctac ggcgaaggtg gcctcggctt gccgtactca cctggcatcc
     3601 cccatccgaa cacgtacaac tgggcctggc gcgaatacgg gaacagggtc ggtggttggc
     3661 gcctcttgga tctcctccag cagagcggaa tacctccgac cgtgctcctg aacacagcct
     3721 gttatgagca ctgccctgag ttggtcgacg cataccgggg cgcaggcgcc gagttcgtcg
     3781 gacatggaca gaccaactcg gtgcacccca acgatctgac cgaggacgac gaacgggaaa
     3841 tgatcaggga cgtcttcgag acaatcctgc gacacgaagg caagcctccg ggcggctgga
     3901 tgagcccggg cgcaaacccg agtgcagtga ccgaagacct actggcggaa accggtttca
     3961 cctataccct ggattggccc atggacgatc agccaacctg gctgcgcacc cggggcggtc
     4021 cactcctcag tgttccctac ccccatgaag tgaacgacgt cccgatgatt gtgttccacc
     4081 acggcaccgc gccggcctac gccgacatga tgaccgacaa cctcgccgag atggtcgagc
     4141 agtcccagca tcagccgctg gtgtgcggca tcgtgaccca cacattcatc atgggccagc
     4201 cctaccggct gcgtcgcttc cggcaggcga tcgagtatct caatggtctc tccggggttt
     4261 ggttgacaac gccgggagag atcgccgctc actacgccgg gatcaccacg ccagaacaag
     4321 ctctcttcgg gagcatcagc acgacatgac ccgggtggcg gtggtaggcc tcggagcaat
     4381 aggccgtgtt gttgtgagcc ggttggtgca gggggtgcct ggggtggagc tgaccgccgt
     4441 ggcctcacgc gacatcgcac gggccgaggc aacaattcga gaccttggcg ccga
//
LOCUS       DBNHXU010000040         1666 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5171606, whole genome shotgun sequence.
ACCESSION   DBNHXU010000040 DBNHXU010000000
VERSION     DBNHXU010000040.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1666)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1666)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1666
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5171606"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..203)
                     /locus_tag="AC161X_01120"
     CDS             complement(<1..203)
                     /locus_tag="AC161X_01120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016499939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HIR4988179.1"
                     /translation="MHRPFGSDSFGRQAESFARFFGTPRFLIGQTVVVVLWISINVVA
                     VTYQWDPYPFILLNLVFSLQAAYA"
     gene            complement(316..720)
                     /locus_tag="AC161X_01125"
     CDS             complement(316..720)
                     /locus_tag="AC161X_01125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013732126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-opening dioxygenase LigA"
                     /protein_id="HIR4988180.1"
                     /translation="MRKAASISSIILGVLLIIGGVATWFSVSGELSEQKIVTSEDACL
                     PGRTVSGPFTAYCEAEVIAKHALEATGGLYYAEMDREDPLRATAATASFLRASLYTSV
                     VAFGSAAMAAGMGIVFILIGLGIRDVEQRTSV"
     gene            892..1659
                     /locus_tag="AC161X_01130"
     CDS             892..1659
                     /locus_tag="AC161X_01130"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4988181.1"
                     /translation="MPPRSFGRTIRYRRTKLGLSQAKLGELVGRSATTIRAWERDASL
                     PTDESVVTALAAVLDVDRQSLFEKAGLEVPVEETSPTLEEAFASLSAQQMPGPTQASL
                     KPAPIAIAPEPEPVIELDIENEPVEVTAEPVVLPAEPSEESTPVGEDYPIWEPDLSAA
                     TLAATTSVASNAVTPSMPAPGFIEPPEPFVLAAATPPMLEPSYMEDTGQRQLYRVRNL
                     ATIVLLVGMGIVLLWAMSSTFDALGSWWQDFVGTLKL"
BASE COUNT          335 a          515 c          499 g          317 t
ORIGIN      
        1 gcgtacgccg cctgcaggga aaacaccaga ttcaagagta tgaacgggta cgggtcccac
       61 tgataggtca cggccacgac attgatcgag atccacagga cgaccaccac cgtctgtccg
      121 attaggaatc gaggggtccc gaagaaccga gcgaacgact cggcttgtcg gccaaacgag
      181 tccgacccga acggccgatg catctgttgt gtgtcgccgt acggcgcaat cgaactattc
      241 accatttgag ctcccgacgt ggaagggttg accggattga cgttaggtcc gatcaaccct
      301 tcggccggga tgaggtcaga cgctggttcg ctgctccacg tcgcggatcc cgagcccgat
      361 caggatgaag acgattccca ttcctgctgc catcgcggcc gagccgaagg cgacgaccga
      421 ggtgtacaag gaggctcgga ggaaggaagc agttgccgcg gttgcccgga gtgggtcctc
      481 ccgatccatc tctgcgtaat agaggccacc ggtggcctcg agggcgtgct ttgcaatgac
      541 ctcggcttcg caataggcag tgaacggccc gctcacggtt cggccgggca ggcaagcgtc
      601 ctcagaggtc acgatcttct gctcggatag ctctccgcta acgctgaacc aggtggcgac
      661 accaccgatg atcaacagca cccccaaaat gatcgacgaa atcgatgcgg cctttctcat
      721 tcttcccttt cttgtggaat gcccgaagtc aacactcttt cgtgcgctcg tgttgagggc
      781 cgaaagtccc tgtgactggg gtcatgggcc atacatgagg gttgggcgaa gatgggatcg
      841 ggtaaggcct atgtgcccaa tccactccca cttgtaccat cgaggatgca aatgccccct
      901 cgttcttttg gaaggaccat ccgctaccgc cgcaccaagc tcggtctgag tcaagccaaa
      961 ctcggcgagc tggtcggacg ctcggccacg accattcgag catgggagcg cgacgcctcc
     1021 ctgccaactg atgagtcggt ggtcacggca ctcgctgcgg tcctcgatgt cgacaggcaa
     1081 tcgctgttcg aaaaggcggg gctcgaggtg ccagtcgagg agaccagccc gaccctggag
     1141 gaggcatttg ccagtttgtc tgctcagcag atgcctgggc cgactcaggc cagcctcaaa
     1201 ccggcgccga ttgcgatcgc tcccgagcct gagcccgtaa tcgaactcga catcgagaac
     1261 gagcccgtcg aggtcaccgc ggagcccgta gtcctgcccg cggaaccatc cgaggagtca
     1321 acgccggtcg gcgaggatta cccaatttgg gaaccagatc tctccgcggc gacattggcc
     1381 gctactactt ctgtagcttc gaatgccgtg acgccatcca tgccagctcc tggattcatc
     1441 gagccacccg agccattcgt cctcgcggca gcaacaccgc cgatgctcga gccttcgtac
     1501 atggaagaca cggggcagcg tcagctgtat cgagtccgga acctggccac catcgtgctc
     1561 ctggtgggaa tggggattgt gttgctctgg gccatgtcga gcaccttcga cgccctcggt
     1621 tcctggtggc aggacttcgt cgggacgctc aagctctagc gttcgg
//
LOCUS       DBNHXU010000041         2929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_410473, whole genome shotgun sequence.
ACCESSION   DBNHXU010000041 DBNHXU010000000
VERSION     DBNHXU010000041.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_410473"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..182)
                     /locus_tag="AC161X_01135"
     CDS             complement(<1..182)
                     /locus_tag="AC161X_01135"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR4988182.1"
                     /translation="MNRPEKLNALSSTLVESLSVALAEAADSEPTRVVILAGAGRSFC
                     AGYDLSEAGGETDIEVG"
     gene            complement(230..2275)
                     /locus_tag="AC161X_01140"
     CDS             complement(230..2275)
                     /locus_tag="AC161X_01140"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HIR4988183.1"
                     /translation="MNSLDPMLLARSVAVVGASERPGSVGDQVVRQLISGGFAGPVHL
                     VNPNYQKLHGLRCVPDLESIGEAVDLVVLAVANAHLEDGLRQAVKVGARSAAIFASCH
                     GEASDGTPLRSRLNQIAEDAALPICGGNGMGFLNIEEGLRVCGFYQPAALVPGGVTFL
                     SHSGSLFSALLHNRRGIRFNLVVSTGLEINTSMDRFLTWALERESTRVVALFLETIRD
                     PAGFRASLEAAAAVDIPVVALKVGVSATGQKAVATHSEALAGDDAVYEALFDACGVHR
                     VASMDEMADTLELLSTGRRATAFGLGAVHDSGGERAMLIDTADRVGVPLPQLTQTSRD
                     RIAAVLDPGLEPANPVDAWGTGRDAEDVFGVCLEALADDPGIGAVAFCVDLTPEEQED
                     GAYSHSAIEVAGKTTKPVMVLGNLTSTLDPYQTGLLREAGVPVLEGTVTGLQAVRHLL
                     ERHRKSSMSALAPRLTPAGDPSSGVDGQLAAFEVLAAYGIPIPALRSVTSEAEALAGA
                     ADIGYPVVLKTAAAVDHKTESQGVVLGIGDEDSLKDAYRDMSEALGTEAIVFGSVPSG
                     VEIGMGMVNDSQFGPVVILSAGGTLIEVIADRVAILPPVDSPRARDALDRLAIRRLFD
                     RVRGGPPMNIDALAEVVIRFSELAIDSAGTVAAIDVNPVIVGPERAVAVDALFKGNA"
     gene            2365..>2929
                     /locus_tag="AC161X_01145"
     CDS             2365..>2929
                     /locus_tag="AC161X_01145"
                     /inference="COORDINATES: protein motif:HMM:NF025343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR4988184.1"
                     /translation="MSPRPSLTESRRQDILAAASAAIAERGLCETRIADIADNIGASP
                     ALILYYFPSKAALLTEALVYQDRLFHESVKGALENASSAVEKFAILIDASCPRPARTG
                     GATDGWTLWPAAWEMSRHDPSLAAARARLDEAWRAQIAAIVDEGIASGEFRPLNSAEF
                     AIQLAAMLDGLAIEVMLGDPTVDADRMR"
BASE COUNT          582 a          974 c          870 g          503 t
ORIGIN      
        1 ccgacctcga tgtcggtctc gccccctgct tcggaaaggt cgtaaccggc gcagaaggag
       61 cgtccggcac cggcaaggat caccacccga gtcggctcac tgtccgctgc ctccgccaac
      121 gccaccgaca acgactcgac gagcgtactc gacagtgcgt tcagcttctc tggccgattc
      181 atggtcagga cacgaaccgg tccccgatct tcagttagca ccagatcagt catgcgttgc
      241 ctttgaaaag ggcgtccacg gccaccgctc tctcaggccc gacgatcacc gggttgacgt
      301 caatggccgc cacagttcct gccgagtcga tcgccaactc ggagaagcgg atcaccactt
      361 cagctagagc atcgatgttc attggtgggc caccgcggac cctgtcgaac agccgccgga
      421 tcgcgagacg gtcgagggca tcccgggccc gcggcgaatc gaccggagga aggatggcga
      481 cccgatccgc aatgacctcg atcagagttc ctcccgcaga cagaatgacc accggaccga
      541 actgggaatc gttgaccatt cccattccga tttcgactcc ggacggaacc gatccgaaca
      601 cgatggcctc ggtgccgagg gcctcagaca tatcgcggta ggcatctttc agagagtctt
      661 cgtctccgat cccgaggacg accccttgcg attcagtctt gtggtcgaca gcggccgccg
      721 tcttgagcac gacggggtat ccgatgtcag cggcccctgc caaggcttcg gcctcagagg
      781 tcaccgaccg aagcgctggg atcggaatgc cgtaggcggc caacacctcg aaggcggcga
      841 gttggccatc aacacccgac gatgggtcgc ccgcaggagt caatcggggt gcgagggcgg
      901 acatcgaact cttcctatga cgctcgagaa ggtgacgcac tgcctgcaat ccggtgacgg
      961 tgccttcaag gaccgggaca cctgcttcac gcaacaaacc ggtttggtag ggatcgaggg
     1021 tcgaggtcag gttgccgagg accatgaccg gcttcgtggt cttcccggcg acttcgatcg
     1081 ctgaatgtga ataggcaccg tcctcctgct cctcgggcgt caggtcgacg caaaaggcga
     1141 cggcgccgat tccgggatcg tcggcgagtg cctcgagaca cacgccgaaa acgtcctcgg
     1201 cgtcgcgacc ggtgccccag gcgtcgaccg ggttggccgg ctccagcccc ggatcgagaa
     1261 ccgccgctat ccgatcacgc gaggtttgtg tgagttgggg caatggcacc cctacccgat
     1321 cggcggtgtc gatcaacatc gctcgctccc caccagaatc gtggactgct cccagcccga
     1381 atgcggttgc ccgtcgcccg gtggatagca actcgagggt gtcggccatc tcgtccatgc
     1441 tcgccacccg atgaacaccg caggcgtcga agagagcctc gtacacggca tcgtcaccgg
     1501 cgagggcctc gctgtgggta gccaccgcct tttgccccgt ggcgctgaca cccaccttga
     1561 gcgctaccac cgggatgtcg accgcggccg ccgcctccaa agatgccctg aacccggccg
     1621 gatcccgaat cgtctccagg aacagcgcca ctacccgcgt gctttcacgc tccagggccc
     1681 aggtcagaaa gcgatccatc gaagtgttga tctcgagacc tgttgagacg acgaggttga
     1741 agcggatacc ccgtcggtta tgcaggagcg cggagaacaa ggatcccgaa tgactgagaa
     1801 aggtcacccc gcccggcacc agagcggcag gctgatagaa cccgcaaacc cgaagaccct
     1861 cctcgatgtt gagaaaaccc atcccgttgc caccacagat cggcaaggct gcgtcctcag
     1921 cgatctgatt gagccgggac cgcaatgggg tgccatcgga ggcttcgcca tggcagcttg
     1981 caaagatcgc tgccgacctg gccccgacct tgaccgcctg cctcagacca tcttcgagat
     2041 gagcatttgc caccgccagg accacgaggt cgactgcttc gccaatactc tcgaggtcgg
     2101 ggacacacct gagcccgtgc agcttctggt agttggggtt caccaagtgg accggacctg
     2161 cgaatccgcc ggagatcaac tgtcggacca cctgatcgcc cactgaccct ggacgttcgc
     2221 tggcaccaac caccgcaacc gaacgggcaa gaagcatcgg atctaatgag ttcatgggtg
     2281 acacatcctg aacggcgatt cagcgtaccg atgggtgatg aatcgtggca acggcacttt
     2341 cctcattgtg ccaatatccc cttcgtgagc ccgagacctt ccctcaccga gagtcggcga
     2401 caggacattc tcgccgctgc gagcgccgca atcgcagagc gaggactctg cgagacgagg
     2461 atcgctgaca tcgccgacaa catcggcgcc agtcctgcat tgattctcta ctacttcccg
     2521 tccaaggcgg ctctcctcac cgaggcattg gtctatcagg accggttgtt ccacgagtcg
     2581 gtgaaggggg ccttggagaa cgcctcatcg gcagtcgaga aattcgcaat cctcatcgac
     2641 gccagttgcc cacggcctgc acgcactggt ggcgccaccg acggatggac cctttggccg
     2701 gcggcatggg agatgtcgcg ccacgatcca tcactcgccg cggcccgagc tcggctcgac
     2761 gaggcttggc gggcccagat cgccgccatc gtcgacgagg gcatcgccag tggggaattc
     2821 cgcccactga actcggcgga gttcgccatc cagctggcag cgatgctcga cggcctcgcc
     2881 atcgaagtga tgctcggtga tcccaccgtc gatgccgacc gcatgcgag
//
LOCUS       DBNHXU010000042         5668 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_806464, whole genome shotgun sequence.
ACCESSION   DBNHXU010000042 DBNHXU010000000
VERSION     DBNHXU010000042.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5668)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5668
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_806464"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..797
                     /gene="rimK"
                     /locus_tag="AC161X_01150"
     CDS             <1..797
                     /gene="rimK"
                     /locus_tag="AC161X_01150"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002651277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="30S ribosomal protein S6--L-glutamate ligase"
                     /protein_id="HIR4988185.1"
                     /translation="CYMDITARRPKVLYRGETLNPEAVVPRIGATYTYYGAAVVRQFE
                     MAGIYSVNESQAISRSRDKLRSLQLLSRAGVGLPTTGFAHATQDIGGLLKVAGGPPVV
                     VKLLEGTQGLGVVLAETTKAAESVIGAFRQLDANILVQEFIKEAGGADIRAFVVGGRV
                     VAAMKRQAPPGEFRSNLHRGGTAEAIKLSPIERSMAIRAAKTMELNVAGVDLIQSSEG
                     PLVIEVNSSPGLEGIEGASGVDVAGEIIEFIENNAGMEPPRGRSRA"
     gene            778..1785
                     /locus_tag="AC161X_01155"
     CDS             778..1785
                     /locus_tag="AC161X_01155"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009544664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004181 - metallocarboxypeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HIR4988186.1"
                     /translation="MVARGPEIRNDPITVGGEVVAPGRRGRIDLPFAKVPTGAQEVLP
                     VAVINGRRPGPHVWLSGAIHGDELNGVEIVRQVMRLLDAKTLRGAVIAVPIVNPLGFI
                     IESRYLPDRRDLNRAFPGSLRGSTASRLAHLFMQEVVSQCSIGIDLHTASNHRINIPQ
                     IRADTHHAATLHLARAFGAPFIIHARVRDGSLRQAATELGATVLVYEAGEAHRFDRDA
                     IEAGVIGVMRTLRSLRMVDIKLPRVGTTRVMRRTRWVRARRGGLANITVELGEEVTKG
                     QVVATISDAFSTRPTTMTAVSSGYVVARTLRPLVNSGDALVHIASQQGPYVDEPAERR
                     R"
     gene            complement(1787..2050)
                     /locus_tag="AC161X_01160"
     CDS             complement(1787..2050)
                     /locus_tag="AC161X_01160"
                     /inference="COORDINATES: protein motif:HMM:NF014520.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR4988187.1"
                     /translation="MFDVKFSPWREGAACIDVVDEVNFFPDPEDLVGIARAKAVCAGC
                     PVADECLTWSIETNQSEGIWGGHTPKERRSVRRRWLEEIEKAS"
     gene            complement(2181..3404)
                     /locus_tag="AC161X_01165"
     CDS             complement(2181..3404)
                     /locus_tag="AC161X_01165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019930353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR4988188.1"
                     /translation="MDFSHSQKVQELQARLGDFMDQHVYPAEALYQEQMATAGDPHHH
                     PEVIEVLKKEARSQGLWNLFLPDPDLGGGLTNLEYAPLAEITGRSPQIAPESINCSAP
                     DTGNMEVLLMFGTDEQKETWLGPLLEGEIRSCFSMTEPDVASSDATNIRTRIDVDGDE
                     LVITGRKWFSSGAASPRCKFSIVMGVTDPDADSYRRQSMVIVPLDTPGVRVEREPTLF
                     GYQDRGGHPQILFDGVRVPATSILGERGGGFAIAQARLGPGRIHHCMRAVGAAERCLD
                     LMVERAQSRTAFGKPLADQGVIQEWVAEARIRIEQARLLVLKTAWMIDTVGAKSARTE
                     IAAIKVVAPSVLTYVADRAIQLFGAAGFTEDFPIAGLYTMGRWLQIADGPDEVHRRSV
                     ARAEIGRARISPAPE"
     gene            complement(3418..4167)
                     /locus_tag="AC161X_01170"
     CDS             complement(3418..4167)
                     /locus_tag="AC161X_01170"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-2 protease family protein"
                     /protein_id="HIR4988189.1"
                     /translation="MGSRSSVGQYEHVLSFRLLGIPVTVRPSFLLVAALLGLQTGSVE
                     LIFAWIAVVFVSILIHEMGHALTARSFGSDVEIELNTLGGLTSWTVPEADFGPGKRAT
                     VAAAGSGVGVVFGGLVWLVASATGPFYGVAGFIVTNLIYVNVFWGLLNWLPIRPLDGG
                     HLFTSLLEKIAPKRGEMIANVVFMVTAAAGLAAALYLRLTFVALLAGYLLFAELTRGR
                     TRQPQVPIPPMSFDRPPPESEPEPKPEEVDG"
     gene            4189..5523
                     /locus_tag="AC161X_01175"
     CDS             4189..5523
                     /locus_tag="AC161X_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015619494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR4988190.1"
                     /translation="MHDDLRAAVAADMPRLKEMLADLVRLPSVSAPGHDQEHVRDAAK
                     RIVAILENSGFHNSQLLEVDGGNPAVFGEVPAPDGAPTVLLYAHYDVQPSGPVSEWRN
                     DPFTPIEQEGRLYGRGSADDKSGVVMHLGAVAAFAGQLPVGVQIFFEGEEEAGSASLE
                     GILEKYSDLLKPDVIVIGDGGMWEVDVPAFLTSLRGIVATTIELRTLKAAVHSGEYGG
                     VFPDALTAMARLLATLHDDDGNVAIPGLVSEEPDGPTIPEARARALIGVVDGVEEIGT
                     GSLASRLWTSPSVSVLAIDAPPVSEAINQLVPVTRAKVSLRVAPHQDTTAALAALKEH
                     LTANAPWGTTVEFLHEEAGHGSVIDVGSQAAEAWRTGFREAYGTEPVEMGAGGSIPFI
                     DTFAALYPGSPILVIGCADHSSAYHAPNESVDLGVVEKSTFAEAIALRVLGG"
     gene            complement(5520..>5668)
                     /locus_tag="AC161X_01180"
     CDS             complement(5520..>5668)
                     /locus_tag="AC161X_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003805026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HIR4988191.1"
                     /translation="PDEVKALPWITQRLRIPMVADVRSLNVANAAAVVVYEAWRQVGF
                     TGAR"
BASE COUNT         1079 a         1793 c         1805 g          991 t
ORIGIN      
        1 gttgctacat ggacatcacg gcccgccgtc ccaaggtcct ctaccgcggg gagacactca
       61 accctgaagc ggtcgtcccc cgaatcggtg ccacctacac ctactacggg gcggcggtgg
      121 ttcgccagtt cgagatggcg ggcatctact cggtcaacga gtcccaggcg atcagccgat
      181 cgcgcgacaa gctccggtct cttcagctgt tgtcacgggc gggagtgggg ctgcccacga
      241 ccgggttcgc ccatgccacc caggacatcg gagggcttct caaggtcgct ggcggcccac
      301 cagtggtggt caagctcctc gagggcactc agggtctcgg tgtggtgctc gcagagacca
      361 ccaaggccgc cgaatccgtg atcggggcct tccggcaact cgacgccaac atcctggtcc
      421 aggagttcat caaggaagcg ggtggggccg acatcagggc gttcgtagtc ggtggaaggg
      481 tggtcgccgc catgaagcga caggctccgc ccggagagtt ccgaagcaac ctccaccggg
      541 gaggaaccgc ggaggcgatc aagttgagcc cgattgagcg gtcgatggcg attcgagctg
      601 ccaagacgat ggagctgaac gtggcgggcg tggacctgat ccagtcgtcc gagggtcccc
      661 tggtcatcga ggtcaactca tctcccggcc tggaggggat cgaaggcgcg agcggtgtcg
      721 acgtcgccgg cgagatcatc gagttcatcg aaaacaacgc cggcatggaa ccaccgcgtg
      781 gtcgctcgcg ggcctgagat tcggaacgac ccgatcaccg ttggtgggga ggtcgttgcg
      841 cctggaagaa gggggcggat cgatctccca ttcgccaagg tcccgaccgg tgctcaggag
      901 gtactgccgg tagcggtcat caatggccgg agacccggtc cgcatgtctg gctctcgggc
      961 gccatccacg gagacgagct caatggagtc gagatcgtgc gccaggtgat gaggcttctc
     1021 gacgccaaga ctctccgcgg cgcggtaatt gccgttccaa tcgtcaaccc tctcgggttc
     1081 atcatcgaat ctcggtatct cccggatcgc cgcgacctga atcgtgcctt tccgggatct
     1141 ctaagaggct cgaccgcctc gcgcctggct catctgttca tgcaggaggt tgtcagccag
     1201 tgctcgatcg ggatcgacct gcacactgct tccaaccatc ggatcaacat ccctcagatc
     1261 agagccgaca cgcaccatgc ggcgaccctc catctggcgc gggcgttcgg agctccgttc
     1321 atcatccacg cccgggtccg tgacggctcg ctccggcagg ccgccaccga gctaggtgcg
     1381 actgtcctgg tctacgaagc gggtgaagcc caccgattcg atcgtgacgc catcgaggcg
     1441 ggggtgatcg gagtcatgcg caccttgagg tcgctgcgca tggtcgacat caagcttccc
     1501 cgcgtgggca ccacgagggt catgcggcgg accagatggg tgcgggcccg tcgcggtggg
     1561 ttggccaaca tcacggtgga actcggtgaa gaggtgacca agggacaggt ggtagcgacc
     1621 atctccgacg ccttctcgac acggcctacg acgatgaccg cggtgtcgag cggctacgtg
     1681 gtagcccgaa cccttcggcc gctggtgaat tcaggggatg ccctggttca catcgcctcc
     1741 caacaggggc cctatgtcga cgagccagcg gagagaagaa ggtgagttac gacgccttct
     1801 cgatctcctc tagccagcga cgacgcaccg agcgtcgctc cttcggggtg tgaccacccc
     1861 aaatgccttc gctctggttg gtctcgatcg accaggtgag gcactcgtca gccaccgggc
     1921 agccggcaca aaccgccttg gcccgggcaa tcccgacgag gtcctccggg tcggggaaga
     1981 agttcacctc atcgacaacg tcgatacagg cggctccttc cctccaggga gagaacttca
     2041 catcgaacat tggtagatcc atctctttgc tgaatcgcgt caacgctttg tccgccttat
     2101 tcgttcccaa aacgggggcg gatgacactt cccacgacct ttcgaacctt acggtgtaat
     2161 accggcggct ccaaaaggcg ctattccggc gcaggcgaga ttcgggcgcg gccgatctcg
     2221 gccctggcta ccgaccgacg atgcacctcg tcgggaccat ccgcgatctg cagccaacgc
     2281 cccatcgtgt acaggccggc gatcgggaag tcctctgtga agcctgcagc gccaaacagc
     2341 tggatggctc gatcggcgac ataagtgagg accgacgggg cgacaacctt gatggcggcg
     2401 atctcggtac gagccgactt cgcccccacg gtgtcgatca tccaggcagt cttgaggacg
     2461 agcagacgcg cctgctcgat ccggatccgc gcttcggcga cccactcctg gatcaccccc
     2521 tggtccgcga gaggcttgcc gaatgcggtt cgggactgtg cccgctcgac catcaggtcg
     2581 aggcaccgct cagcagcgcc aactgccctc atgcagtggt gaatgcgccc cggtcctaga
     2641 cgggcctgag cgattgcgaa tcctccaccc cgctcgccga gaatgcttgt cgcaggtact
     2701 cgcaccccat caaagagaat ctgagggtgg ccgccccgat cctgatagcc gaacagggtg
     2761 ggttctcgct cgactctcac cccgggcgtg tcgagcggaa cgatcaccat cgactggcgc
     2821 cgatacgagt cggcatcagg gtcggtgact cccatcacga tggagaactt gcaacgcgga
     2881 gaggccgcgc ccgagctgaa ccacttccga ccggtgatga ccaattcatc accatcgacg
     2941 tcgatccggg ttcggatgtt cgtggcatcg gaactggcga catcgggctc ggtcatcgag
     3001 aaacaggacc gaatctcacc ctccagcagt ggtcccagcc aggtctcctt ctgctcgtcg
     3061 gtcccgaaca tgagcagcac ctccatgttc ccggtgtccg gcgccgagca attgatcgac
     3121 tcgggggcga tctgagggct gcggccggtg atctcggcca gcggggcata ttcgaggttg
     3181 gtgagccctc cccctaggtc ggggtcggga agaaacaggt tccacaggcc ttggctgcgg
     3241 gcttccttct tgaggacctc aatgacctcg gggtgatgat ggggatcgcc cgcagtcgcc
     3301 atctgctcct gatagagcgc ttctgctgga tagacgtgct ggtccatgaa gtcgccgagt
     3361 cgggcctgta actcctggac cttttgtgaa tgagagaaat ccatcggtgc gccgaaccta
     3421 gccgtcaacc tcttcgggct ttggctccgg ttcggattcg ggaggggggc gatcgaagct
     3481 catcggcggg atcggaacct gaggttggcg ggtgcgccct ctggtcagct cagcaaacag
     3541 caggtatcca gccaagaggg cgacgaaggt gagccggagg tagagagccg cggccaaccc
     3601 ggccgccgcg gtgaccatga agaccacgtt tgcgatcatc tcgccgcgtt ttggtgcgat
     3661 cttctccaga agagaggtga agaggtggcc accatcgagc ggtctgatcg ggagccagtt
     3721 gagcaggccc cagaacacgt tgacgtagat gaggttggtg acgatgaacc cggccacccc
     3781 atagaaagga ccggttgccg aagccaccag ccaaaccagg ccgccaaaga cgacgccaac
     3841 accagaaccg gcggcggcca ctgtggcgcg cttccctgga ccgaaatcgg cttcaggaac
     3901 cgtccatgag gtcaagccgc caagggtgtt caactcgatc tccacatccg agccgaagct
     3961 gcgcgccgtg agggcatgcc ccatctcgtg gatgaggatc gagacgaaga ccacggcgat
     4021 ccaggcaaag atcaactcaa cgctgcccgt ctggaggccg aggagggcgg ccaccagaag
     4081 gaaggacggc cggacggtga ccgggatccc gagtagtcga aacgaaagca cgtgctcata
     4141 ttggccgacc gaggacctgc tgcccaacga ggccgttagc ctcggcacat gcacgatgac
     4201 cttcgggcgg ctgtcgccgc cgacatgcca cgtctcaaag agatgcttgc cgatctggtt
     4261 cggctcccct cagtcagcgc accaggacac gatcaggaac acgttcgaga tgcagcgaag
     4321 aggatcgtcg ccattctcga gaattccggc ttccacaact cgcagctcct cgaggtggac
     4381 ggcggcaatc ccgcggtgtt cggcgaagtg cctgcacccg atggagcgcc caccgttctt
     4441 ctctatgccc actacgacgt gcagcccagc ggtccggtca gcgaatggcg gaacgacccc
     4501 tttactccca tcgaacaaga ggggcgcctc tacggtcgcg gctcggccga cgacaagtca
     4561 ggcgtcgtca tgcacctcgg cgccgttgcc gcctttgcgg gacagctacc ggtaggggtg
     4621 caaatcttct tcgagggtga ggaagaggca ggctccgcca gtctcgaagg catcctcgag
     4681 aaatactccg accttctgaa gccagacgtg atcgtcatcg gagatggcgg gatgtgggag
     4741 gtggatgttc cggccttcct cacgtcgcta cgcggaatcg ttgcgaccac catcgaactg
     4801 aggaccctca aagcggcggt ccactccggt gagtacggag gcgtgtttcc ggatgcactg
     4861 accgccatgg cacggctgct ggccactctt cacgacgacg acgggaacgt cgcgatccca
     4921 ggcttggtga gcgaggagcc tgatggcccc accatccccg aggcgagggc gcgggccctg
     4981 atcggagtcg tcgatggagt cgaggagatc gggaccggct cgctcgcctc ccggctctgg
     5041 acgagcccgt cggtttcggt gctcgcgatc gatgcacccc cggtgagcga ggcgatcaac
     5101 caattggtgc cagtcactcg ggcgaaggtc agtttgcggg tcgcccccca ccaggacacc
     5161 accgcggccc tggccgcatt gaaggagcac ctcaccgcca acgcaccctg gggaacgact
     5221 gtggagtttc ttcacgaaga ggccgggcac ggctcggtaa tcgacgtagg gagccaggct
     5281 gccgaggcct ggaggaccgg atttcgagaa gcctacggaa cggagccggt ggagatgggt
     5341 gccggtggat cgattccgtt cattgacacc ttcgccgcgc tctaccccgg ctcaccgatc
     5401 ctggttatcg gctgcgccga ccattccagc gcctatcacg ctcccaacga gagcgtggac
     5461 ctcggtgtcg tcgagaagtc gacttttgcc gaggcgatcg cgctgagggt gttaggtggt
     5521 tagcgggcgc cggtaaaccc gacttgccgc cacgcctcgt agaccaccac cgcggccgcg
     5581 ttggccacat tcaatgagcg gacgtcggcg accatcggga tcctcaaacg ctgtgtgatc
     5641 caggggagcg ccttgacttc gtcgggca
//
LOCUS       DBNHXU010000043         4834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1798946, whole genome shotgun sequence.
ACCESSION   DBNHXU010000043 DBNHXU010000000
VERSION     DBNHXU010000043.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4834
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1798946"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..558
                     /gene="hpt"
                     /locus_tag="AC161X_01185"
     CDS             40..558
                     /gene="hpt"
                     /locus_tag="AC161X_01185"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HIR4988192.1"
                     /translation="MDIKPLITADDINAKLAEMGKRITDDYSGEDLLLVGVLRGAFMV
                     MADLARHIDLPVEFDFMAVSSYGASTQTSGVVRILQDLDEEIEGRHVLIVEDIIDSGL
                     TLNYLMKSLNVRKPASLEVATLLLKEGIQRVPIGVKYVGFPIGPEFVVGYGLDYAGKY
                     RNLPYVGVMGGE"
     gene            634..2634
                     /gene="ftsH"
                     /locus_tag="AC161X_01190"
     CDS             634..2634
                     /gene="ftsH"
                     /locus_tag="AC161X_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018192130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HIR4988193.1"
                     /translation="MNRTLRTLVVYGLSIILLAVLAQWWFDQVSGPEEVTLSQFMEKV
                     DAGTFTEVQVLARSNQVQGRVEPGTDTEDTWDFVASYPEGSEGQITEAILATGVDVSA
                     DPEPPTFWELLISFLPWLFLLGFMVFIFMQMQGGGNRVMQFGKSRAKLVGKGQPKVTF
                     DDVAGLEEAKEELEEIKDFLANPDRYRQIGAKIPKGVLLFGPPGTGKTLLARAVAGEA
                     GVPFLSISGSDFVEMFVGVGAARVRDLFEQAKASSPAIVFIDEIDAVGRHRGAGLGGG
                     HDEREQTLNQLLVELDGFEGNTGVIVMAATNRPDILDPALLRPGRFDRQIVVDAPDIE
                     GRKAILEVHSKGKPLAKEIDLEVLARRTPGFTGADLANLINEAALLTARRHKKEVSML
                     ELEEAIDRVIAGPERKTKVMSDEERKLIAYHETGHALVGYAMPNSDPVHKVTIVARGR
                     SLGHTLALPTHDKYLVRRSELVDQLAMLLGGRTAEEIIFADPTTGAANDIEKATAIAR
                     RMVMEYGMSETLGPMQYGKPNGEVFLGRDYSRQQDYSDDVAAKVDEEVRKLITAAHEE
                     AREVLHRHRDAMERMVEQLLETETVGQDDVAKLFNDVPKWEHTAEGAMRIKYPDNPVT
                     PQPREQIAAAEKVAEEEEEPVTESLRLKRSLRPKGRPADAGA"
     gene            complement(2631..3404)
                     /locus_tag="AC161X_01195"
     CDS             complement(2631..3404)
                     /locus_tag="AC161X_01195"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR4988194.1"
                     /translation="MTEQTFDVGDSPRFVASISSGSVEVVESPAGVISLRIESSREAE
                     FEVSHSGDTVTVRRPPNGGGWSRREAHTRLRVAVPIGSTVRISCSSADVTTEVRLNEA
                     YIDTASGDVAIWEVKNAKVKTASGEVAIGTATGDVSLRTASGDVKVGDATGRVEISTA
                     SGSVDLGTTRGDVTSSTASGDLDIERYEGSDLDLKSASGSIRIGLPAGTKVALDANSM
                     SGRIRLPERTPSGISRADRHQVRARLRTISGDIELVRLG"
     gene            complement(3401..3838)
                     /locus_tag="AC161X_01200"
     CDS             complement(3401..3838)
                     /locus_tag="AC161X_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988195.1"
                     /translation="MHLDHVFIEIENAVDNQVRIGSPELAEVATELMTALRPTFERAM
                     LGVIQEAAAEMSAQLVGQSVEVRLSEGEPSLVLTENRKTPTNDPEELEARVTVRLPSS
                     LKSLIEESATSTGDSMNGWVVDALRTRAGRKARGTHVEETFEL"
     gene            3833..3955
                     /locus_tag="AC161X_01205"
     CDS             3833..3955
                     /locus_tag="AC161X_01205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988196.1"
                     /translation="MHQRRLVVRRTRSESSLSRLSALLIQNIGNLLGYDIHVMS"
     gene            4048..4623
                     /gene="folE"
                     /locus_tag="AC161X_01210"
     CDS             4048..4623
                     /gene="folE"
                     /locus_tag="AC161X_01210"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019226266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HIR4988197.1"
                     /translation="MKEIDQGRIEKAVREILEALGEDPEREGLIETPERVARFYLEVF
                     DGLHRDPGDEIDAFFGEERYQEIVMVREIPFYSMCEHHLVPFHGQAHVAYMPKGRVTG
                     LSKLARLVEGYARRPQMQERLTAQVADALAERLEPLGVMVVIEAEHLCMSMRGVRKPG
                     AKTVTSAVRGIMHTNPATRAEAMSLLFGTTR"
BASE COUNT          899 a         1472 c         1519 g          944 t
ORIGIN      
        1 ccgcggctac ctttccgttt ccgccagaga ggaatccgga tggacatcaa gcccctgatc
       61 accgccgatg acatcaacgc caagctcgcc gagatgggca agcgcatcac tgacgattac
      121 tcgggagagg atcttctcct cgttggcgtg ctgcgcgggg ccttcatggt gatggcggat
      181 ctggcacgtc acatcgacct acccgttgag ttcgacttca tggcggtttc ctcatacggg
      241 gcatccacgc agacctcagg cgtggttcga attctccagg acctcgacga ggagatcgag
      301 gggcgtcatg tcctcattgt cgaggacatc atcgattccg ggttgacgct gaactatctg
      361 atgaagagcc tcaatgtccg caaacccgct tcgctggagg tggcgacctt gctcctgaaa
      421 gaggggatcc agagggttcc gatcggggtc aagtacgtcg ggttccccat cgggcccgag
      481 ttcgtcgtcg ggtatggatt ggattacgcg ggcaagtacc gcaatctccc ctacgtcggg
      541 gtcatgggcg gcgaatgaga gagggttcga ctattcggcg tggtcccgta acattgaggg
      601 ttcgagagct ccctttctga aattgaggtc tttgtgaacc gaactctgcg cacgctggtg
      661 gtctacgggt tgtcgatcat cctgttggct gtccttgccc agtggtggtt cgaccaggtc
      721 tcggggcctg aggaggtgac cctctctcag ttcatggaga aagtggacgc cgggaccttc
      781 accgaggtgc aagttttggc ccgctccaac caggtgcagg gtcgggtcga accggggacc
      841 gacaccgaag acacctggga ctttgtggcc agctaccccg aagggtctga gggccagatc
      901 accgaagcga tactcgccac cggcgtggac gtcagcgccg atccggagcc gcccacgttc
      961 tgggagctgc ttatcagctt cctgccgtgg ctgttcctgc tcggcttcat ggtgttcatc
     1021 ttcatgcaga tgcaaggcgg cgggaaccgg gtcatgcaat tcggcaagtc ccgggccaag
     1081 ctcgttggca agggacagcc gaaggtcacc ttcgacgatg ttgccggcct cgaggaggcc
     1141 aaggaggaac tcgaggagat caaagacttc ctggccaacc ccgatcggta tcgccagatt
     1201 ggggccaaga tcccaaaggg cgtcctcctc ttcggccctc ccggcaccgg caagacgctg
     1261 ctcgccagag cggtcgcggg tgaggccggg gtcccattcc tctcgatctc tggatcggac
     1321 ttcgttgaaa tgttcgtcgg tgtcggcgct gccagggtga gagacctctt cgagcaggcc
     1381 aaggcgagct ccccggcgat cgtgttcatc gacgagatcg acgcagtggg acgtcatcgc
     1441 ggcgcgggtc tgggtggtgg ccatgacgag cgggagcaga ccctcaacca gctgctggtc
     1501 gagctcgacg gcttcgaggg aaacaccgga gtgatcgtca tggcggctac caaccgcccc
     1561 gacatcctcg accctgcttt gctccggccc gggcgcttcg accgtcagat cgtggttgac
     1621 gctcccgaca tcgaggggcg caaggcgatc ctcgaggtcc attcgaaagg caagcccctg
     1681 gccaaggaga tcgatctcga ggtcctggcc cggcgcaccc ccggattcac tggagctgat
     1741 ctcgccaacc tgatcaacga ggccgctttg ctgactgccc ggcgtcacaa gaaagaggtc
     1801 tcgatgcttg agctcgagga ggcgattgac cgtgtcatcg ccggccccga gcgcaagacc
     1861 aaggtgatga gcgacgagga gcgcaaactc atcgcctacc acgagaccgg tcacgccctg
     1921 gtcgggtatg cgatgcccaa ctccgacccg gtgcacaagg tgacgatcgt ggcccgcggt
     1981 cgatcattgg ggcacacgct ggcccttccc acgcacgaca agtacttggt gcgaaggtcg
     2041 gagctggtgg accagttggc aatgctgctg ggtggccgca ccgcagaaga gatcatcttc
     2101 gccgacccca ccaccggcgc cgccaacgat atcgagaagg caactgcgat cgcccgccgc
     2161 atggtgatgg aatacggcat gagtgagact ttgggcccca tgcagtacgg caagcccaac
     2221 ggtgaggttt tcctcggtcg ggactactcc cgtcagcagg actactcgga cgatgtcgcg
     2281 gccaaggttg atgaagaggt tcgcaagctg atcaccgcag cccacgagga ggcccgtgag
     2341 gtgctccatc gacatcgtga cgcgatggag cgaatggtgg agcaactctt ggagaccgag
     2401 acagtcggcc aggacgacgt tgcgaagctc ttcaatgacg tcccgaagtg ggagcacacg
     2461 gccgaaggcg cgatgcggat caagtacccg gacaacccgg tgacgcccca gccccgggag
     2521 cagatcgccg cggccgaaaa ggttgccgag gaggaagaag aaccggtcac cgagtcgctc
     2581 cgcctcaaga ggagtctgcg gcctaagggc cgtcccgccg acgccggcgc ctaacccagc
     2641 cgcacgagtt cgatatcacc tgagatcgtt cgcagcctgg ctctcacctg atgtcgatcc
     2701 gcacggctga ttcctgaggg agtgcgctcc ggaagtcgga tccgacccga catcgaattt
     2761 gcgtccaacg cgaccttggt ccccgccggg aggcctatcc gaatgcttcc ggatgccgat
     2821 ttgaggtcga ggtcgctgcc ttcatagcgc tctatgtcga gatctcctga ggcggttgac
     2881 gaggtcacgt ctcctcgggt cgtgccgagg tcgacggatc cggaggcggt gctgatctcg
     2941 acccgtcccg tcgcatcgcc gaccttcacg tcgccagacg cagtcctcaa actgacatca
     3001 ccggtggcgg ttccgatggc cacctcgccg ctggcggtct tcaccttggc gtttttgact
     3061 tcccaaattg cgacatcacc agatgcggtg tcgatgtagg cctcgttgag tcgaacttcg
     3121 gtggtgacgt cggctgagga gcatgaaatc ctgacagtgc tgccgatcgg gactgccacg
     3181 cggagtcgag tatgtgcttc gcgccgactc cagccgccac cattgggcgg ccggcgcacg
     3241 gtgacggtgt cgccagagtg gctcacttcg aactctgcct cccgcgagct ttcgattcgg
     3301 agactgatga cgccggccgg gctctcgacg acttcgacgg atcccgaact gatcgaggct
     3361 acgaaacggg gggagtcacc cacatcaaat gtctgctcgg tcacagttcg aaggtctcct
     3421 cgacatgggt gccccgcgcc tttctcccgg cacgggtgcg cagtgcgtct acgacccagc
     3481 cgttcatcga atcaccggtc gaggtagcgg actcttcgat gagagatttc aatgagctcg
     3541 gcagtcggac cgtgaccctc gcttcgagct cctcggggtc gttggtcggc gtcttccggt
     3601 tctcggtgag taccaggctt ggttcccctt cgctcagtcg aacctcgaca ctctggccga
     3661 ccagttgggc cgacatctcc gccgcggcct cctggatgac accaagcatc gcccgctcga
     3721 aggtaggccg caacgccgtc atcagctcgg tggcgacctc ggccaactcg ggggagccga
     3781 tcctcacctg gttgtcgacg gcgttctcga tctcgatgaa cacgtggtcg agatgcatca
     3841 gcgccgtctc gtggttcgcc ggacccggtc ggagagctcg ctttccagac tgtctgcgct
     3901 tctgatccaa aacatcggaa atctcctcgg ttatgacatc catgtgatgt cgtagtgatg
     3961 tcaaggtaat gtcaccatga cgtcgttgtc aagtctgata tcgttgcttt gggaatatgt
     4021 ggcccagagc gcctattttc accagggatg aaagagatcg accagggacg aatcgaaaag
     4081 gcggtccgcg agatccttga ggccctgggg gaggatcccg agcgtgaagg tctcatcgag
     4141 accccggagc gggtggcccg gttctatctc gaagtgttcg acggtctcca tcgcgatccg
     4201 ggagacgaga tcgacgcctt cttcggcgag gagcgctacc aggagatcgt catggttcgg
     4261 gagatcccgt tctactcgat gtgtgagcat catctcgtgc cgttccacgg tcaggcgcat
     4321 gtcgcctaca tgcccaaggg ccgggtgacc gggctttcca aactggctcg tttggtcgag
     4381 ggatatgccc gccgccctca gatgcaggag cgtctgaccg cccaggtggc cgacgccctc
     4441 gccgagcgtc tcgaaccgct cggcgtgatg gttgtgatcg aggccgagca cctgtgcatg
     4501 tcgatgcgcg gggtgagaaa accgggagcc aagacggtga catcggccgt gcgggggatc
     4561 atgcatacca accccgccac ccgcgccgag gcgatgtcgc tgctcttcgg caccacccgc
     4621 tagctcgctt cgctcgccgt tatacgttct actttttgcg ttcagaggag actgagcagg
     4681 tgagccaata ctggcaactg ggtgacaact tgctcgacgt gtcccatggc gtcctgatgg
     4741 gagttctcaa tgtcaccccc gactcatttt ccgatggagg gatgcacgcc acggcctctg
     4801 atgcagtcgc agccgggatc gcgatgagcg atgc
//
LOCUS       DBNHXU010000044         3390 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4974187, whole genome shotgun sequence.
ACCESSION   DBNHXU010000044 DBNHXU010000000
VERSION     DBNHXU010000044.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3390)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3390)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3390
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4974187"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..227
                     /locus_tag="AC161X_01215"
     CDS             <1..227
                     /locus_tag="AC161X_01215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4988198.1"
                     /translation="ANRKTPAASLVPSKLNEIAAPTYYVWSQDGEVPRESVDSILAAL
                     PAMQVEVVPAWKAHLESPEVVANAILTVAS"
     gene            complement(267..683)
                     /locus_tag="AC161X_01220"
     CDS             complement(267..683)
                     /locus_tag="AC161X_01220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988199.1"
                     /translation="MDDRVLVAIEVPLLGDERHKNWAKIVDSVDTSKSSGWAFDGPFV
                     ATGGIQDVPAGSVLLVYGERGSRVNPQITARVFIVNADATITMEGEAKGKAWARTIRD
                     TVERCLGMEKSPLDLSHLSDELLADELRARGWQVEK"
     gene            752..1753
                     /locus_tag="AC161X_01225"
     CDS             752..1753
                     /locus_tag="AC161X_01225"
                     /inference="COORDINATES: protein motif:HMM:NF014636.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HIR4988200.1"
                     /translation="MAKLRLFANLREIAGSARVDVGSGTVGGAIEEVSVRYGDDFRRT
                     VATSRVWVNGETASYDDPVGANDEMVILPPVSGGNRSMTAPATTLDFMAFLPFAVIAI
                     AVLANLQSQQIWAAALVAIVAAWALDLGAVMSSRGRMFAPLAVVVTSAVSAMAAHVMG
                     GTGYSLALVVAVVVALGWAVAFPPYRQVDVVSPLLLVSLFAGLGTASMILARSAHSPD
                     PRAVDVFLVSVIAGLVLGSIVERMPSVPVVDPFSMTAVGAVLGAVGAAFLWDLDVVGY
                     LLIGLGIAVALVAGRGFASMLRTGKVALTERPPGWLSSVDGAVLAAAIFFPLLAVIL"
     gene            1828..2913
                     /locus_tag="AC161X_01230"
     CDS             1828..2913
                     /locus_tag="AC161X_01230"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR4988201.1"
                     /translation="MAAARTHHRVVLLVTVTALVASLFPLATQAVTQSQVNEACADSR
                     QQLDDYRAAQAEFEDAALEYEGALNEVEKVEVKQDRVQGSMTVHTDDLDRIQQQIEEQ
                     AVQLYMQGGLSSPGIILSASSVDQLMTTSEFLTQAATGGQRSIDELIAARSELDRFNS
                     DLSDTRAELKVVEDHKLDAMNRQESAMEAEQAAYAKLSDRCKTLTSKYDTEQRARAAA
                     AKQRASGSVQVGSFICPITPGRSSFINSWGFPRSGGRRHKGTDMMTNYGEPLYAVTSG
                     TIRISHSSLGGNSIWLRGNNGNSFYYAHLSDYNVGSGAQVSQGQTIGFAGTSGNAAGG
                     APHLHFEIHPGGGAAANPYPTLAAACR"
     gene            2924..>3390
                     /locus_tag="AC161X_01235"
     CDS             2924..>3390
                     /locus_tag="AC161X_01235"
                     /inference="COORDINATES: protein motif:HMM:NF046924.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsiG family protein"
                     /protein_id="HIR4988202.1"
                     /translation="MSGLRRIDTVLEPQFLVGLDAIELDELRARRDTAEDVENQISYY
                     RRLLHGRMDLLNFELRRRSGEEQRSLLDALPEILASGMVLGPEPNLRHIDVMPPLPTT
                     TGRRLIDQIMDDGILTQLPELTDEDLDEAVERLREVEAELSNQRRQLHTVIDAL"
BASE COUNT          593 a         1090 c         1038 g          669 t
ORIGIN      
        1 gcgccaatcg caagacgccc gcggcctcat tggtcccgtc aaagctcaac gagatcgcag
       61 cgcccaccta ctacgtgtgg agccaagacg gtgaggtgcc ccgggagtcg gtcgattcga
      121 tcctcgcggc gctgcctgcg atgcaggtcg aggtggttcc cgcctggaag gcccatctcg
      181 agtccccgga agtggtggcc aacgcaatcc tgacggtggc ctcctaggag tcaaggactc
      241 cttaggcgcc cacgcttgga gggcgactac ttctcaacct gccaaccacg cgccctcagt
      301 tcgtcggcca gcaactcgtc gctgagatga ctcagatcga gaggcgactt ctccatcccg
      361 agacagcgct cgaccgtgtc gcggatcgtt ctcgcccaag ctttgccctt ggcctcgcct
      421 tccatggtga tggtggcgtc tgcgttgaca atgaacacac gggcggtgat ctggggattg
      481 acccgcgaac cgcgttcgcc atacaccagg aggacagatc cggccggcac gtcctggatg
      541 ccgcccgtcg ccacgaacgg gccatcgaac gcccatccgg aagacttgct ggtgtcgaca
      601 gagtccacga tcttggccca gttcttgtga cgctcgtctc cgagcagcgg aacctcgatg
      661 gccaccagca ccctgtcgtc catggggacg agagtacgca atcagacgcg tgaacccgcc
      721 tcctgggacg aagacgcttt acccttgacc catggccaag ctgcgtctgt tcgccaatct
      781 ccgtgaaatt gccggctcgg ccagagtcga tgttggatcc ggcacagttg gtggggccat
      841 cgaggaggtc tcggtgaggt acggcgacga ctttcgccgc acagtggcca cctcacgggt
      901 ttgggtcaac ggtgagactg cttcctatga cgatcctgtt ggtgccaacg atgagatggt
      961 catcctgcct ccagtctccg gcggcaatcg ttcgatgacg gccccagcaa ccaccctcga
     1021 cttcatggca tttctcccat ttgcggtgat cgcgattgcg gtcctggcca acctccagag
     1081 tcagcaaatc tgggccgcgg ccctggtggc gatcgttgcc gcatgggccc tcgatctcgg
     1141 cgccgtgatg tcatcccgcg gccggatgtt cgcccctctt gcggtggtgg tcacttctgc
     1201 agtctccgcc atggcggctc acgtgatggg tggcaccgga tactccctgg ccctggtggt
     1261 tgccgtggtg gttgccctgg ggtgggcggt cgcatttcct ccgtaccgtc aggtggacgt
     1321 cgtcagcccg cttctgctcg tttctctgtt tgcgggattg ggcacggcgt cgatgatcct
     1381 ggcgcggtca gcccatagcc ccgacccgag agcggtcgat gtcttcctcg tgtcggtgat
     1441 cgccgggttg gttttgggtt cgatagtgga gcgcatgcca tcagtcccgg tcgttgaccc
     1501 gttctcgatg accgccgttg gcgcggtgct tggcgccgta ggtgccgcct tcctttggga
     1561 cctcgacgta gtcggctacc tcctcatcgg cctcgggata gcggtggctc tcgtcgcggg
     1621 gcgggggttc gcctcgatgc tccgcaccgg aaaagtggca ttgaccgagc gaccaccggg
     1681 ctggctcagc tcggtcgatg gggccgtatt ggccgccgcg atcttctttc ctttgctcgc
     1741 ggtcatcctc tgacccatct cggggtggcg ggccgcttgt caggatggac tagagcaaag
     1801 aaccgggccg ggttacgctt tcatcgcatg gctgctgcgc ggacgcacca tcgcgtcgta
     1861 ctgctcgtca ccgtgaccgc cttggtggcc tctctgttcc cccttgccac gcaggccgtc
     1921 acgcagtctc aggtcaatga ggcctgcgct gactcgcgcc agcagctcga tgactatcgg
     1981 gcggctcagg cagaattcga ggacgcggcc ttggagtacg agggagccct caacgaggtt
     2041 gaaaaggtcg aggtcaagca ggacagggtt cagggatcga tgacagttca caccgacgat
     2101 ctcgatcgca tccagcaaca gatcgaggag caggccgtcc agctgtacat gcagggcggc
     2161 ctttccagtc ccgggatcat cctctccgcg tcatcagtcg atcagttgat gacgacttcc
     2221 gagtttctca cccaggccgc caccggcggc cagcgttcga tcgacgagtt gatcgcagcc
     2281 aggagcgagc tcgatcggtt caacagcgac ctctcggaca cccgcgccga gctcaaagtc
     2341 gtggaggacc acaagctcga cgcaatgaac cgccaggagt cggctatgga ggccgagcag
     2401 gccgcctacg ccaagttgtc agacagatgc aagaccctca cttcgaaata cgacaccgag
     2461 cagcgggcca gagcggccgc agccaagcag cgggccagtg gctcggtgca ggtcgggtcg
     2521 ttcatctgtc cgatcacccc cgggcgctcg tccttcataa attcttgggg attcccgaga
     2581 tcgggcgggc gacgccacaa agggaccgac atgatgacca actacggaga gcccctttac
     2641 gcggtgacct cagggaccat tcgcatttcg catagctcgc tgggtggtaa ttcgatctgg
     2701 ttgcggggca acaacgggaa ttccttctat tacgcccatc tctccgacta caacgtcggg
     2761 tctggagccc aggtgagcca ggggcagacc atcggcttcg ctgggacctc cggaaacgcg
     2821 gccggcggtg cccctcatct tcatttcgag atccaccccg gcggaggcgc tgcggcgaat
     2881 ccctatccga cccttgccgc ggcctgcagg taacctctac acagtgagcg gactccgccg
     2941 aatcgacacc gtcctcgagc ctcagtttct cgtcggcctc gatgccatcg aactcgatga
     3001 gttgagggcg aggcgggaca ccgcagagga cgtcgagaac cagatctcct actaccggcg
     3061 cctcctccac ggtcggatgg acctgttgaa cttcgagctt cgtcggcgaa gtggcgagga
     3121 gcagcggtcc ctcctcgacg cacttcccga gatcctcgcc agcggcatgg ttctcggtcc
     3181 tgagcccaac ctccgacata tcgacgtgat gcctccgtta cccacgacca cgggtcgtcg
     3241 actgatcgac cagatcatgg acgatgggat tctcacccag ctccccgaat tgaccgatga
     3301 ggacttggac gaagccgttg agcggttgag agaggtcgaa gcggaattgt ccaaccagcg
     3361 caggcagctt cacaccgtca tcgatgctct
//
LOCUS       DBNHXU010000045         3811 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1998342, whole genome shotgun sequence.
ACCESSION   DBNHXU010000045 DBNHXU010000000
VERSION     DBNHXU010000045.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3811)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3811)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3811
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1998342"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="AC161X_01240"
     CDS             complement(<1..656)
                     /locus_tag="AC161X_01240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR4988203.1"
                     /translation="MSEALENLLSEDRKFPPPPEFTAAANAQPGIHESAAADYLGFWQ
                     EQALSRVTWATEPTVVLDDSNHPFYKWFSDGELNISVNCLDRHLEDHGDQVAYNWVGE
                     PGDTRVLTYSDLHAEVCRFANGLKSLGVETGDRVAIYMGMVPELPIAMLACARLGLVH
                     SVVFGGFSAEALSSRIVDADARLVITQDGAWRAGNVVPLKVNSDLAVSQTPGVEHVVV
                     V"
     gene            complement(653..2149)
                     /gene="lnt"
                     /locus_tag="AC161X_01245"
     CDS             complement(653..2149)
                     /gene="lnt"
                     /locus_tag="AC161X_01245"
                     /EC_number="2.3.1.269"
                     /inference="COORDINATES: protein motif:HMM:TIGR00546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HIR4988204.1"
                     /translation="MTGLLLVVGGAALAGLAFPRIGPGWLILPGVALFLAGLRTAANR
                     RQGLMLGAAYGLVFNAILLWWFVELGLVAVIPLVLYQATFWAAYGWWLSKYNDRGSGT
                     WFLLVVGGWAVTELIRYHMPVLSFEWGAVGYGLSDQVWARNFAPMYGTTGVTVLAVMT
                     AAGLVAVWRRPRNWWVLVPVPVLALGFWLSQFNPTEINVFADFPVAIVQGSTPCPFEH
                     CPPDERLRTFQQHLELTKTIQPGDVSLVVWAEGSTGGFNADPVQNPEVGEAIAAEARR
                     IGAWFLVGSDRPISETHWENVNIVFNPEGEIVGEYQKQHPVPFGEYIPIRPLFEWIPA
                     LDQVPRDMIPGDNPVVFDLGDGLKMGSVISFEGGFARYPRQHVEEGANFIVIATNEAS
                     YGTTPGSDQFIGMTRMRAAELGLDVVHAAVTGKSTFIDASGAYGEETGLGTQEVLYGG
                     VAVGRTPSIYARTSDVLMITAAVIGLITWWRARKPLVASADPEEDESA"
     gene            2192..3001
                     /locus_tag="AC161X_01250"
     CDS             2192..3001
                     /locus_tag="AC161X_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012673.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HIR4988205.1"
                     /translation="MWEGDLWVAVRAARAGAEVIRRGFEEPFDTEMKGVVDPVTDIDR
                     EAEAAVTEVISDHFPDDSILAEEGGGSANWRGARVWIIDPLDGTVNFVNRVPHVAVSV
                     ALWDNGEPLVGVVIDVSRQEEFVAAVGEGARSNGQAIHVSNTASLEDSLLATGFPYDR
                     HQHAGAYLDAMEQVMTRSRDSRRMGAAALDMAWVACGRFDGYWEHSGPHGVKPWDAAA
                     AMLLVTEAGGTATNSKGGVNDLEPSAFVVTNGKIHEDLRTIIDDHMPAHLR"
     gene            3001..>3811
                     /locus_tag="AC161X_01255"
     CDS             3001..>3811
                     /locus_tag="AC161X_01255"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR4988206.1"
                     /translation="MFHDHHFHPMGYAQMKTGLELMDSADLEQVKARVSARAEQTSGA
                     IIGQRLNDEGLAELRLPTRTDLDEAVPDRPVVLYRYCGHIAVANSEALRLAGIDATTP
                     DPPGGAFDRDRTGFPTGVLRETALLVIGNAIAPLAEAPSDGDVLEALRGLLDYDLGSV
                     TGMVSATDPIWCGVGDELEALCRMAPELPLDINVYVITENPSDLVSAAKRIAEADGRL
                     SFSGWKGFADGSFGGHTASMYEPFSDKPDATGTLRLYPGQALTMADASLEIG"
BASE COUNT          753 a         1238 c         1191 g          629 t
ORIGIN      
        1 accaccacca cgtgctcgac cccaggagtc tggctcaccg cgaggtcgct attgaccttg
       61 agcgggacga cgttgccggc acgccaggcc ccgtcctgag tgatcaccaa tcgggcatcg
      121 gcgtcgacaa tcctgctgga gagagcctcg gcagagaagc cgccaaacac caccgaatgg
      181 accagcccga gccgggcgca ggccagcatc gctatcggga gctcggggac catccccatg
      241 taaatggcaa ctcggtcgcc ggtctcgacc cccaggctct tcaacccgtt tgcgaaccgg
      301 cagacttcgg cgtgaagatc ggagtaggta aggacccggg tatcaccagg ctcccccacc
      361 cagttgtaag cgacctgatc gccatggtct tcgagatggc gatccagaca gttgacggag
      421 atattcagct caccatcgga aaaccacttg tagaaggggt gattcgagtc gtcgagcacc
      481 accgtgggct cggttgccca ggtgacccgg gaaagcgcct gctcctgcca gaacccgaga
      541 tagtcagcag cggccgactc atgaatcccc ggttgggcat tggcggcggc ggtgaactcc
      601 ggcggtgggg ggaacttgcg gtcttcggaa agcaggttct ccagggcctc gctcatgcgc
      661 tctcatcctc ctcggggtcg gcggaagcta ccagaggctt gcgagcccgc caccaggtga
      721 tgagaccgat gacggctgca gtgatcatca ggacgtcgct ggtgcgggcg tagatagatg
      781 gggtgcggcc gactgccacg cctccgtaga ggacctcctg ggtccccagc ccggtctcct
      841 cgccatatgc gcctgacgca tcgatgaatg tggacttccc ggtcacggcg gcgtggacaa
      901 catcgagccc caattcggcg gcccgcatcc tggtcatccc gatgaattga tcggagccag
      961 gagtggtccc ataggaggcc tcgttggtgg cgatcacaat gaagttcgct ccctcctcga
     1021 cgtgttgtct cggatatcgc gcgaacccac cttcgaatga gatcaccgag cccatcttga
     1081 gaccatcacc gaggtcgaat accaccggat tgtcgccggg gatcatgtcc cggggtacct
     1141 ggtcgagagc cggtatccat tcgaataggg gacgaatcgg gatgtactcc ccaaacggga
     1201 cagggtgctg tttctgatac tcaccgacga tctccccctc ggggttgaac acgatgttca
     1261 cgttctccca atgggtttcg ctgatcggtc gatccgaccc gaccaggaac cacgctccta
     1321 ttcgtcttgc ttcggccgcg atcgcctctc cgacctccgg gttctgtacc gggtcggcgt
     1381 tgaaaccgcc ggtggagccc tcggcccaca ccacaaggga cacgtcacct ggctggatgg
     1441 tcttggtcaa ctcgaggtgc tgctgaaaag tccggagccg ctcgtcgggc gggcagtgtt
     1501 cgaaggggca gggagtcgag ccttgaacga tggcgaccgg aaagtctgcg aacacattga
     1561 tctccgtcgg gttgaactga gacaaccaaa atccgagggc caacaccggc accggaacca
     1621 aaacccacca attgcgcggc ctcctccaaa ccgccaccag gccggcggcg gtcattactg
     1681 ccagcaccgt caccccggtc gtcccataca tcggggcgaa attccgcgcc cacacctgat
     1741 cggagagccc gtaacccacc gctccccatt cgaaggagag gactggcatg tgatagcgga
     1801 tcaactcggt gacggcccag ccgccgacca cgagcagaaa ccaggtgccc gacccacggt
     1861 cgttgtactt cgacaaccac cacccatagg ccgcccagaa agtggcctgg tacaacacca
     1921 gagggatgac cgccaccaga cccaactcca cgaaccacca gagcaagatg gcgttgaaga
     1981 ccaggccgta ggccgcgccc agcatcaaac cctgcctgcg attggcagcg gtccgtaagc
     2041 cagccaggaa cagcgcaacg ccgggcagga tcagccaccc gggaccgatt cggggaaagg
     2101 cgaggccggc gagagcagcc ccaccgacaa ccagcaacag gccagtcacc ggcgagaggg
     2161 tagggatttc acgttccgta gagtgccggg catgtgggaa ggtgacctct gggtcgcggt
     2221 cagagcggcg cgcgccggcg cggaggtgat tcggcgcggc ttcgaggagc cattcgacac
     2281 tgaaatgaag ggcgtggtcg acccggtgac cgatatcgat cgggaagcgg aagccgcggt
     2341 caccgaggtc atctcagacc acttccccga tgactcgatc ctcgcggaag agggcggtgg
     2401 cagtgccaat tggcgcggtg cccgggtttg gatcatcgat cctctcgacg ggacggtgaa
     2461 cttcgtgaac cgcgtaccgc atgtcgcagt ctcggtggca ttgtgggaca acggcgaacc
     2521 attggtcggg gtggtgatcg atgtcagccg tcaggaggaa ttcgtggccg cggttggaga
     2581 aggggcgcgc agcaacgggc aggcgatcca tgtgtcaaac accgcatctc tcgaggactc
     2641 actgctggca accggttttc cctatgaccg tcaccagcat gccggcgcct atctcgatgc
     2701 catggaacag gtgatgaccc gatcccggga ttcccgacgc atgggcgccg cagccctcga
     2761 catggcttgg gtggcctgtg gtcgcttcga tggttactgg gagcactccg ggccacacgg
     2821 cgtgaagcca tgggacgcgg cggcggccat gctcctggtc accgaggcag gcgggaccgc
     2881 caccaacagc aagggcgggg tcaacgacct cgagccgtcc gcctttgttg tcaccaacgg
     2941 caagatccat gaggacctga ggacgatcat cgacgaccac atgccggccc atctccgatg
     3001 atgttccacg accaccattt ccatccgatg ggctacgccc agatgaaaac cgggctcgaa
     3061 ctgatggact cggcggacct ggagcaggtc aaggctcggg tctccgcgag ggccgaacag
     3121 acctcggggg cgatcatcgg ccagcgtctc aatgacgagg gcctcgccga gctccggctc
     3181 cccacgcgaa ccgatctcga tgaagccgtg cccgaccgcc cggtggttct ctatcgctac
     3241 tgcgggcaca ttgcagtcgc caacagcgag gcgttgcgac tcgccgggat cgacgcgacg
     3301 acgcccgacc cgccaggcgg ggcattcgat cgggaccgca ccggattccc caccggggtc
     3361 ctcagggaaa ccgccctgct ggtcatagga aacgccatcg cccccctcgc cgaagctcca
     3421 tccgacggag acgtcctcga ggcgttgcga gggctcctcg actacgacct gggctcggtg
     3481 accgggatgg tctcggcaac cgatccgatc tggtgcgggg tgggtgacga gttggaggcc
     3541 ctctgccgga tggcgcccga gctccccctc gacatcaacg tctatgtgat caccgagaac
     3601 ccctccgatc tggtctccgc cgccaagcgc atcgccgaag ccgatggccg actcagcttc
     3661 tccggttgga agggcttcgc cgatgggagt ttcggggggc ataccgcctc gatgtacgag
     3721 ccgttttccg acaagccgga cgccaccggc accctgcgcc tctaccccgg acaggcgttg
     3781 accatggccg acgcctctct cgaaatcggg g
//
LOCUS       DBNHXU010000046         2653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2395053, whole genome shotgun sequence.
ACCESSION   DBNHXU010000046 DBNHXU010000000
VERSION     DBNHXU010000046.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2653
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2395053"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1043
                     /locus_tag="AC161X_01260"
     CDS             <1..1043
                     /locus_tag="AC161X_01260"
                     /inference="COORDINATES: protein motif:HMM:NF014342.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DHHA1 domain-containing protein"
                     /protein_id="HIR4988207.1"
                     /translation="LTVDSGFIAVGQTATAVIDSPRREAIRKSHTGTHVLHWALRDVL
                     GEHAKQAGSLVEPGRLRFDFSHFAQVAPQELSEVEAEVNQRLIENASVQTTVTSKEQA
                     EQMGAIAFFGDKYGETVRVVQVGDFSVEFCGGTHTHSSAQVGPLLITSESSIGSNLRR
                     LEALTGLAAYQRMAEVRHGMDRAGQLLKTGPADVPDRVEALLERVSGLEKDLEAMRGS
                     QRGELATELSDSSTVIGETSLVVADAENMDANSLRQLALGVRDRLGGRSLVVVGSRHD
                     GKGVLIGLVSKDLVAAGISAAEVISEAARELGGGGSRDPELAQAGGQHGERLPQALEL
                     AREAAERALAAL"
     gene            1040..1444
                     /gene="ruvX"
                     /locus_tag="AC161X_01265"
     CDS             1040..1444
                     /gene="ruvX"
                     /locus_tag="AC161X_01265"
                     /inference="COORDINATES: protein motif:HMM:NF015607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HIR4988208.1"
                     /translation="MSRCLGVDVGTKRVGLALSDPIGLTATPLDVVERSEAVDEIIRL
                     AQIHEVGTIVVGLPTSLGGGEGTSAVDARSLGEELAQATSAEIVYLDERFTSRMAESA
                     LLESGMNRRDRKNTIDKVAAAIILQDYLNSLQ"
     gene            1441..2541
                     /gene="mltG"
                     /locus_tag="AC161X_01270"
     CDS             1441..2541
                     /gene="mltG"
                     /locus_tag="AC161X_01270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR4988209.1"
                     /translation="MTFEPVPEPSPWSRAGRMIGIAGIVVLSAIAVVGGASYLGRVVG
                     SSLNSEDPTAGPVNVVPGQEVTVEIPSGSSAEDIAAILAAQGVVRSALEFEVAVRNNE
                     AASRLQAGTYELETLMDPAEVVAVLVVGPVADVYRVTVIEGLRVGEILTRLSEASGVP
                     YEQFEQALLSGDVSTSLRTMPATMALADWEGLLFPDTYEFTKSAEPVAILQRLASTME
                     QRVDSIDWSVVEAAGFTPYEGLIIASLVETEVLLDEERPIVSSVIHNRLAIDMKLDID
                     ATVLYALGTRDIAEFNNEVDSPYNTYVVAGLPPTPIASPGRASLEAAAAPDTTDYLYY
                     VLSDLEGHHAFAATLDEHNANVEQARADGVLP"
BASE COUNT          459 a          852 c          860 g          482 t
ORIGIN      
        1 gcctgaccgt cgactcaggt ttcatcgcgg tgggccagac cgcgaccgcg gtcatcgact
       61 cgccgcgccg ggaggcgatc cggaagagcc acaccgggac ccatgtcctg cactgggcgc
      121 tccgcgatgt cctcggcgaa catgcgaaac aggccgggtc gttggtcgaa ccggggcgtc
      181 tccgcttcga tttctcccac ttcgcccagg tagcccccca ggagctttcc gaggtcgagg
      241 ccgaggtcaa ccagaggctg atcgagaacg ccagtgtcca aaccacggtc acctcgaagg
      301 aacaggcgga gcagatgggg gcgatcgctt tcttcggcga caagtatggg gagaccgtga
      361 gggtggtgca ggtcggcgac ttctcagtcg agttctgcgg agggacccat acccactcgt
      421 ctgcgcaggt gggcccgctg ctcatcacct cggagtcctc gattgggtcc aacctgaggc
      481 ggctggaggc actcaccggt ttggccgcct atcagcggat ggccgaagtc agacatggca
      541 tggaccgagc aggccagctg ctcaagaccg ggccggcaga cgtcccggac cgggtcgagg
      601 cgctcttgga gcgggtcagc ggcctggaga aggacctcga agcaatgcgt ggctcccagc
      661 gcggagagct cgccactgag ctgtccgact cgtccactgt gattggcgag acatccctgg
      721 tggttgcgga tgccgagaac atggatgcca actccctccg tcagctcgca ctcggggtca
      781 gggatcgcct ggggggtcgt tcgctggtgg tcgttggctc gcgtcatgac ggcaagggcg
      841 ttttgatcgg gctggtctcc aaagacctgg tagcggctgg tatttctgcg gccgaagtca
      901 tcagtgaggc cgcacgcgag ctcggtggtg gcggttcccg cgatcccgag ctggctcagg
      961 ccggtggcca gcatggcgag cggcttcccc aggccctgga gcttgcccgc gaagccgcgg
     1021 agcgcgccct cgcggccctg tgagccgctg tctcggggtt gacgtcggaa ccaagcgagt
     1081 gggactggcg ttgtccgatc ccatcgggct caccgcaact ccattggatg tcgtcgaaag
     1141 gtcagaggcc gtggacgaga tcatccggct cgctcaaatt cacgaggtgg gtaccatcgt
     1201 ggtcggtctc cccaccagtc tcggcggtgg tgagggcacg tctgccgtcg acgctaggag
     1261 tctcggcgaa gagctggccc aggccaccag cgccgagatc gtctatctcg acgagcgatt
     1321 cacctcgcgg atggcggaga gcgcccttct cgagtccggc atgaaccgtc gggaccgtaa
     1381 aaacaccatc gacaaggtgg ctgccgccat catcctccag gactatctga actctttgca
     1441 atgactttcg aaccagtccc tgagccatcg ccctggtcgc gcgccgggcg aatgatcggc
     1501 atcgccggca ttgtggtcct atccgccatc gccgtcgttg gtggagcctc ctacctcgga
     1561 cgggtcgtgg gatcctctct caactccgag gacccaaccg cagggccggt caacgtcgtg
     1621 cccggccagg aggtgactgt ggagataccg agcggctcca gcgccgagga cattgccgcc
     1681 atcctcgctg cgcaaggcgt cgtccgctct gccctcgaat tcgaggtggc agttcgcaac
     1741 aacgaggcgg cttcccggct ccaggcgggg acctacgaac tggagactct catggatccg
     1801 gcggaggtcg tggcggtcct ggtggtcggt ccggtggccg acgtgtatcg ggtcaccgtt
     1861 atcgagggcc tccgggtagg ggagatcctc acccggctct ctgaggcgtc gggggttccc
     1921 tacgagcagt tcgaacaggc tttgttgtcc ggggatgtgt cgacctcgct gcgaacgatg
     1981 ccggcgacca tggctctcgc agactgggaa ggcctgctct tccccgacac ctacgagttc
     2041 accaaatccg ccgagcccgt tgcgatcctc caacggcttg cctcgacgat ggaacagcgt
     2101 gtcgacagca tcgattggtc ggtcgtcgaa gctgccggat tcaccccata cgaggggttg
     2161 atcatcgctt cgctggttga gaccgaggtg cttctcgacg aggagcggcc gatcgtctcg
     2221 agtgtgattc acaaccgttt ggcgatagac atgaagctcg acatcgatgc cacggtgctt
     2281 tacgcactcg gcacccggga catcgcggag ttcaacaacg aggtcgactc gccctacaac
     2341 acctatgtgg tcgccggcct gccacccacc cccatcgcat cgccgggtcg ggcttcactg
     2401 gaggcggcag ccgctcctga caccaccgac tacctctatt acgtgctctc agatctcgaa
     2461 gggcaccatg cctttgcagc cacgctcgat gagcacaacg ccaatgtcga gcaggctcgg
     2521 gccgacggag tccttccctg agcacggacc cgggtcccag acggcgatga gagccgacct
     2581 gagatttgcg ctcctcggcg acccggtcga tcactcccga tccccggcaa ttcacagggc
     2641 agcgctcaca gag
//
LOCUS       DBNHXU010000047         1741 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2197804, whole genome shotgun sequence.
ACCESSION   DBNHXU010000047 DBNHXU010000000
VERSION     DBNHXU010000047.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1741)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1741
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2197804"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="AC161X_01275"
     CDS             complement(<1..771)
                     /locus_tag="AC161X_01275"
                     /inference="COORDINATES: protein motif:HMM:NF015655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988210.1"
                     /translation="MTDPRNATPAALAEERLRETVPDPVIRRELAESVSSGPDPSGAL
                     DRLLPILEADPELPADPEARRRLTALVGSSRALSRSLSRNPWLLQEDSQETVTLQLHH
                     ALATIAGDDLSGAIDLSESVARYSDAVDRIVSHALDRARVDIAERHPIVEALPFAVIA
                     MGKWGARELNYSSDVDFVLIHDNVEKSEVASRSAALALASRLVTVLSTPTSDGAALEI
                     DLDLRPEGSIGPLSRTLESYARYYEQWGEAWELQALLKA"
     gene            807..>1741
                     /locus_tag="AC161X_01280"
     CDS             807..>1741
                     /locus_tag="AC161X_01280"
                     /inference="COORDINATES: protein motif:HMM:NF012993.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR4988211.1"
                     /translation="MPVRVAGGQLNLTVGDLEGNEARIAEVITWAESQGADVLLLPEL
                     AINGYPPEDLVLRRDFVKAGIEARDRLAERSGRMAVVIGFVDFSDPVVRGAPDSQERS
                     VANAAALLQHGKVVGVYHKVLLPNYGVFDEDRYFSVGTRPDATWDLDGTLVGVSICED
                     IWVPEGPPAQQAASGARLLLNINASPFHRGKALDRERMIIERAQASSTPVVYLNLVGG
                     QDELVFDGASLVVSAEGAVLHRSPQFEEDLFIVDVPLAGEDAGATSLTPLLDPIEEVY
                     RALATGLGDYVRKNGFRSVVIGLSGGIDSALTAVIA"
BASE COUNT          285 a          525 c          596 g          335 t
ORIGIN      
        1 tgccttgagc agcgcctgca gctcccatgc ctccccccat tgctcgtagt agcgggcgta
       61 actctccaat gtgcgggaga gcggcccgat tgagccttcc gggcgcagat cgaggtcgat
      121 ctcgagagca gcaccgtcgc tcgtcggcgt cgagaggacg gtcacgagcc ggctcgccag
      181 ggccagggct gctgatcggc ttgcgacctc cgacttttcg acgttgtcgt gaatcaagac
      241 gaagtcgacg tccgatgagt aattcaactc gcgcgctccc cacttgccca tggcgatgac
      301 ggcgaagggc agggcttcga cgatgggatg cctctcggcg atgtcgacac gggcccggtc
      361 gagtgcgtgc gagacgattc ggtccaccgc gtccgaatag cgggccacgg actcagacag
      421 gtcgatcgct ccggacaggt cgtcgccggc gatggtggcc agggcgtgat gcagctgcag
      481 ggtcacggtc tcctggctgt cctcttggag caaccacgga tttctggaga gactccggct
      541 caaagctcga ctggatccga cgagggccgt caggcgtcgt cgggcttcgg ggtcagccgg
      601 cagttcgggg tcggcttcga ggatggggag caagcggtcg agggcacccg aggggtccgg
      661 tccggatgag acactctctg cgagctcacg ccgaatcaca gggtcgggaa ccgtctcgcg
      721 caatctttcc tcggccagcg ctgccggcgt cgcattcctc ggatcggtca cagggctcag
      781 gctaagggga ggcgtatcgt ggcggtatgc cagttcgggt cgccggggga caactcaatc
      841 tgacggtcgg cgacctcgaa ggcaacgagg cgcgaatcgc tgaagtcatc acgtgggcag
      901 agagccaggg tgcggacgtc cttttgctcc ctgaattggc catcaacggg taccccccgg
      961 aggatttggt gctccgtcgt gacttcgtga aggcgggaat cgaagcccgt gatcggctcg
     1021 ccgagcggtc gggccgcatg gccgtcgtga tcggtttcgt ggatttctcc gacccggtgg
     1081 ttcggggagc cccggattcc caggaacgat ccgtggccaa tgcagcggcc cttctccagc
     1141 atgggaaggt cgtcggtgtc tatcacaagg tgctcttgcc caactacggc gttttcgatg
     1201 aggaccggta cttctccgtc ggcaccaggc cggacgcgac ttgggacctg gacgggacgc
     1261 tggtgggggt ctcgatctgt gaggacatct gggtgcccga agggccgccg gcacagcaag
     1321 ccgcctcggg ggcccgcctc ctgctcaaca tcaatgcctc tccatttcac cggggcaagg
     1381 ctctcgacag ggagcgaatg atcattgaga gggctcaggc cagttccacg ccggtggtct
     1441 acttgaacct ggtcggtggc caggacgagt tggtctttga tggggcgtcg ttggtcgtct
     1501 cagctgaagg ggccgtactc catcggtcgc cccagttcga ggaggatctc ttcatcgtcg
     1561 atgtccctct cgccggtgaa gacgcagggg cgacgtcact gaccccgctg ctcgacccga
     1621 tcgaggaggt gtatcgggca ttggcgaccg gcctcggtga ctatgtccgc aagaacgggt
     1681 tccgatctgt cgtgatcggc ctctccggcg gcatcgattc ggctctcact gcggtgatcg
     1741 c
//
LOCUS       DBNHXU010000048         1857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1008070, whole genome shotgun sequence.
ACCESSION   DBNHXU010000048 DBNHXU010000000
VERSION     DBNHXU010000048.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1857
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1008070"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..843
                     /locus_tag="AC161X_01285"
     CDS             <1..843
                     /locus_tag="AC161X_01285"
                     /inference="COORDINATES: protein motif:HMM:NF019626.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine/phenylalanine carboxypeptidase
                     domain-containing protein"
                     /protein_id="HIR4988212.1"
                     /translation="HILEGLAGPIMEMEDDRSIDGNTAVGILQAQLDRSMGPGKIEVM
                     ADDGIAADAAAGADYIKLNEDKFFSQRDLDLLEAHEGWVHVGTTLNGLAQPVCTFLGK
                     AAPRSTVTQEGLAVLTEMINMRSHPRRLAKLMHRIEGIKMATEGATFIEVFEAFRTDG
                     LSEDEAWSAAHRIFRGSLPDAGPFTKDLGYGKGLVLTLLYVRLAIQTGKAHRIPLLFC
                     GKVDLLDMSVLHQLEEEGLVEQPEFVPPPFNDLPALSVALSLGGFMAKLDWTRLEADY
                     SSLI"
     gene            869..>1857
                     /locus_tag="AC161X_01290"
     CDS             869..>1857
                     /locus_tag="AC161X_01290"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4988213.1"
                     /translation="MWANRDVRRVVAGRFISRVGGEAAFFVGIWGKTAFELEASATGV
                     AAVMAALGLASLIGSAIAGVLVDRYDPRRVLIWGELLFIPVALSMVFANTIPTMVVAT
                     FALGLVGTPVYTAIASFGPYLTDDPEKLAKVNGYIEGASWAAFVVGPALGAVITITVG
                     LNGIFVLDAATSAIAVVLMIPVTVRKLDKKEQDGSHGFQELRDGFRYSYGSRRLRFYL
                     ILGSLVWILFGTFSALEPLFYRDVLGVAPETMGWVNSLFGIGLVTGTLISSRIPPRLR
                     TARTMVVLVALNSVGDLLYIGTNRLPVVVAAGMTWGVVIGTMAPLLRTMIQINS"
BASE COUNT          311 a          568 c          595 g          383 t
ORIGIN      
        1 cacatcctcg aggggctggc agggccgatc atggagatgg aagacgatcg ctccattgat
       61 gggaacacgg cggtggggat cctgcaggcc caactcgaca ggtcgatggg tcccggcaag
      121 atcgaggtca tggccgacga cgggattgcg gccgatgcgg cagccggggc cgactacatc
      181 aagctcaacg aggacaagtt cttctcccag cgcgacctcg acctgctcga agcccatgag
      241 gggtgggtgc atgtcggaac cacgctcaac ggccttgccc agccagtctg caccttcctc
      301 gggaaggcgg cgccccggtc cacggtgacc caggaggggc tcgcggtgct caccgagatg
      361 atcaacatga ggagccatcc ccggaggctg gccaagctga tgcaccgtat cgagggcata
      421 aagatggcca ccgagggtgc aacgttcatc gaggtctttg aggcctttcg gactgacgga
      481 ctgagcgaag acgaggcatg gtccgcggcc catcggatct tccgaggcag ccttcccgat
      541 gccggcccct tcaccaagga cctgggctac ggaaagggcc tggtgctcac gctgctctat
      601 gtgcggctcg ccattcagac cggaaaagct caccgtattc cgttgctctt ctgcgggaag
      661 gtggatctcc tcgacatgtc ggtgctccat caactggagg aggaaggcct cgtcgagcaa
      721 ccggagttcg tcccaccacc gttcaatgac cttcctgctc tcagcgtcgc gttgagcctg
      781 ggcgggttta tggccaagct cgattggacc aggctcgaag cggactattc cagtctgatc
      841 tgagcgtccc ggcggtaccg tggccgccat gtgggccaat cgtgacgtca ggcgggttgt
      901 cgccggtcgg ttcatatccc gggtgggggg cgaggctgcc ttcttcgtag ggatctgggg
      961 caagaccgcc ttcgagttgg aggcttcggc aactggcgtg gccgcagtta tggcggccct
     1021 gggcctggca tctttgatcg gctcggcgat tgctggagtt ctggtcgatc gatatgaccc
     1081 gcgccgggtg ttgatctggg gtgagctgct tttcatcccg gtcgccctat ccatggtgtt
     1141 cgccaacacc atccccacga tggtggtggc caccttcgcc ctggggctgg tcgggacccc
     1201 cgtctacacg gctattgcat cgtttggccc gtatctcacc gacgaccccg agaaactggc
     1261 gaaagtcaac ggctacatcg aaggcgcatc ctgggcggcg ttcgtcgtcg gcccggccct
     1321 cggggccgtg atcacgatca ctgttggcct caatgggatc tttgtcctcg atgccgccac
     1381 ttctgcgata gcggtggtgc tgatgatccc ggtcactgtg cgcaagctcg acaagaagga
     1441 acaagacggg tcccacggtt tccaggagct tcgagatgga ttccgctact cctacgggag
     1501 tcgccggctg cggttctatc tgatcctcgg gtccctggtc tggatcctct ttgggacctt
     1561 cagtgcgttg gagcccctct tctatcgcga tgtgctcggg gtcgcacccg agaccatggg
     1621 atgggtcaac tccttgttcg ggatcggcct cgtgaccggc actttgatct cctcgcggat
     1681 accaccgcgg ctcaggacag cccgcacgat ggtggtcctg gttgccctca actcggtggg
     1741 cgacctcctc tatatcggca ccaaccggct tccggtggtg gtggctgccg ggatgacctg
     1801 gggagtcgtg atcgggacga tggcccctct cctccggacc atgattcaga tcaactc
//
LOCUS       DBNHXU010000049         7250 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1008411, whole genome shotgun sequence.
ACCESSION   DBNHXU010000049 DBNHXU010000000
VERSION     DBNHXU010000049.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7250)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7250)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7250
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1008411"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /locus_tag="AC161X_01295"
     CDS             <1..1011
                     /locus_tag="AC161X_01295"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR4988214.1"
                     /translation="LLAPVWGFSDLSSGAFAVVGMAAMFAVVGRAPLTAILLVFEVTG
                     ARDYQLILPLMLTATLATFLAERFHPESIYSMALKGMGISVRRTGEVDVLDTIDVGAV
                     MTRPAVVASPADTLIALEKSLREAQSHGAPVVDNGSLVGIITISDIARFGEDSDSSQV
                     GDAMTPRPATVTASTRVSRALERMAALGVGRLPVVAEDDPAKLVGIFRREDAVRAYHD
                     ALTAATDVELHRARLAQRTGAGAGYYEFRVPPGSIADGRAIREISWPEGSTLVSIRRE
                     RDVMVPSGDTILLRGDLVTAFGTYTSKDRMIERLNEGADEATAEVSLAEIKSEGESGE
                     PA"
     gene            complement(994..1887)
                     /locus_tag="AC161X_01300"
     CDS             complement(994..1887)
                     /locus_tag="AC161X_01300"
                     /EC_number="3.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012703.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginase family protein"
                     /protein_id="HIR4988215.1"
                     /translation="MKSGFAMIGVPVDSAGGPGGTELAPAALRSLGLGEAIHGRDEGD
                     LEVRIRAEGRDPVTGVVALDDVCATTAEVRRAVAETLAKEERPFLVGGCCSIVPGALA
                     GVRDARGAASLVHLDGHLDLYDSTTSPTGEAADMPVTVALGLGPAAWVQAAGGPSLSG
                     GDVWILGYRDREQSEVDGMIMPEDVDPPIACLTTDEMRSLGSGDVGRRTAGALANGVG
                     NIWVHLDLDIVDPYLFFANDAPVPNGLDWTELTELLSAVCSSPALVGISLGCYNPEKD
                     RNQENGAQIVEVFRKALGGFA"
     gene            1979..2908
                     /locus_tag="AC161X_01305"
     CDS             1979..2908
                     /locus_tag="AC161X_01305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988216.1"
                     /translation="MEGVGAAVIAIVVLAVVVVSLRRQRKRAAAVDLEDTSWLRRAPD
                     EPEPLTKRLAVGEFHVSGHEARVVFDVPLPEEEDPILNELLVDEAVEVVREKRHSLPI
                     DDVTEIVVFAGRGNSREIGRTTLPAPGQLPVAIQPDMLNLTHIARDPFASQFEDDPMA
                     QVETRVDVPADDLGPIRNELRIPLGLERGIRTRGVDPATASGPELILALLEMFGYEVS
                     PQVDGTFIATKDKVKTFIRAEPHDSGSHPELDEQVVQKFIVEFGTSGATRGLLISDKL
                     GPFMIHDVEAMNPRVRFVTRQRIQRFIDSMAIG"
     gene            2964..3158
                     /gene="tatA"
                     /locus_tag="AC161X_01310"
     CDS             2964..3158
                     /gene="tatA"
                     /locus_tag="AC161X_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011744466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0043953 - protein transport by the Tat
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HIR4988217.1"
                     /translation="MLGLRGQEWLIILLIVLLIFGARKLPDLARSLGASAKEFRKGID
                     EGTETESDTQTPEPPPPSDS"
     gene            3201..3704
                     /locus_tag="AC161X_01315"
     CDS             3201..3704
                     /locus_tag="AC161X_01315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019853233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR4988218.1"
                     /translation="MTESVPLTWEEVARVYGRKIYNFAYRLTGNPDDAADLVQEVLLR
                     VRRGLASYQPGSFEGWLWRITRNAFLDDVRKRNRRPIAPLPDEIDRWELASTPGSDVE
                     YSRISLGDDIQKALLEVPIEFREAVVLCDVIGLSYEEIAIAVSVPIGTVRSRIHRGRK
                     ILKDLLS"
     gene            3701..4114
                     /locus_tag="AC161X_01320"
     CDS             3701..4114
                     /locus_tag="AC161X_01320"
                     /inference="COORDINATES: protein motif:HMM:NF024881.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor family protein"
                     /protein_id="HIR4988219.1"
                     /translation="MTHPTELISAYLDGELTRAEVSQLQAHMSSCGKCVADMEGLQKV
                     RTSVRSLPLIELPPGLLGDAEPVVVPLRRNRGFWVGAAAAGVAVVIAVATMLTPQPTS
                     LSVNDLNSRFGARVSLDPAFGPAKVAVPDVRDLTE"
     gene            4111..5019
                     /locus_tag="AC161X_01325"
     CDS             4111..5019
                     /locus_tag="AC161X_01325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988220.1"
                     /translation="MRRAVVVGVLLTLLPATAASAADLEELLDRSHDASYSAEQVISC
                     NTPEGARDAVVSLEQVGGELQVSGEVEVSSGSGGWALTSEGSVVDSASVEAGTNGAEP
                     IYRVDDGVANPFLDRKAGLYRLYAGDVLRGELIVDGATGALLSVVTYNADGEVYCDRR
                     FIEFDPTAPDVAVPSVASGEALEPSEDAAAFPERLGTFERLDIYEDGEGLVFAYYSDG
                     FFSFAVFKTPSVVELTDPGSLTLEGLVYGRSFSPGQVTYSWETRNGGMALVGDLPPDL
                     HPEILAGLDQPFSPGILGRLWRNLFG"
     gene            complement(5020..6657)
                     /locus_tag="AC161X_01330"
     CDS             complement(5020..6657)
                     /locus_tag="AC161X_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR4988221.1"
                     /translation="MKQDAIAGLPGAIGSVPDGMASAVLVGVNPVYGLYASFAGPIGG
                     GLTASTRLMVITTTTAAALAAGSALSSIDPEDQAASLFLLVLMAGAIMILAGVLKLGR
                     YTRFVSHSVMIGFLTGVAANIIFGQIPDFTGAESVGANSLAKALNVLLHPSSIDPVSL
                     LTGLAAVALIVILGRTPLSSYASIIALVVPTILVLGVESVARVEDVGDIPQGLPIPAL
                     PDITLLTPSLVVAALAIAAIVLVQGAGVAESAPNRDGSTSNPDRDFIAQGVGNLASGF
                     FRGLAVGGSVGQTALNISAGARDRWASISSGIWMLVILVVFSGVVGVVAMPTLAAILI
                     VAAIGSLRIGQIESILRTGPNSQIALISTFFATLFLPIAAAVAFGVVLSLMLQLNQEA
                     IDLRVVEWVPGPENSFAEQPAPRSLESQHVTILNAYGSLFYAGARTLQARLPEIGGAS
                     EPVVILRMRGRIELGATAFKVLDDYAGRLEAAEGRLYLSGIEPRVMSHFMRAGHVSAQ
                     GPIQLYEATPNIGESTLAALQDAEAWIVEHRSADDDA"
     gene            complement(6705..>7250)
                     /locus_tag="AC161X_01335"
     CDS             complement(6705..>7250)
                     /locus_tag="AC161X_01335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988222.1"
                     /translation="QLVVLVGLYLMPFAGIAFLWFAVALRMWLSRMTSTQDVLLSNVQ
                     LVSAIIFIALFLAGAAASTVLAVSVEFSDYPVDPGAARQFPQFGSALLLVFSMRMAAM
                     FVFTTSAIARRHKVLPRWFVWAGYVVGLFLLLVATLNRLLVLVFPIWILILSVILLLK
                     ARRIPREGQEPTPDGAVGAST"
BASE COUNT         1372 a         2231 c         2348 g         1299 t
ORIGIN      
        1 ttattggcgc cggtatgggg attctccgat ctcagcagtg gggcatttgc ggtcgtcggg
       61 atggcggcga tgttcgcggt ggtgggaagg gcgcccctca ccgcgatcct cttggttttc
      121 gaggtgaccg gggcccggga ctaccagttg atcctgccct tgatgctgac ggccacgctg
      181 gccacattcc tcgccgagcg atttcatcct gagtccattt actcgatggc tctcaaggga
      241 atgggcatca gcgtccgtcg aactggagag gttgacgttc tcgacacgat tgatgtgggc
      301 gccgtgatga cgcgtcctgc agtggtggcc tcgcccgccg acaccctgat cgccttggag
      361 aagagtctgc gtgaggccca atctcacgga gctccggtgg tcgacaacgg cagtctggtc
      421 gggatcatca ccatctccga catcgcccgg ttcggcgagg actcggacag cagccaagtc
      481 ggcgacgcaa tgacaccacg tcccgccacg gtcacggcgt cgacacgagt ttcgagggcg
      541 ttggagcgaa tggccgccct cggcgtcgga cggctacctg tcgtggccga ggacgatccc
      601 gccaagttgg tcggtatctt caggagggag gatgcagtcc gcgcctatca cgacgcccta
      661 accgcggcca ccgacgtcga actgcaccgc gctcggctcg cccagcgcac cggcgccggc
      721 gccggttact acgagttcag agttccgccg ggctcgatcg cagacgggcg tgcgatccgc
      781 gagatttctt ggccggaggg gtcgacgttg gtatcgatca ggcgggaaag ggacgtgatg
      841 gttcccagtg gcgacacaat cctgcttcgc ggggatctag tcaccgcctt tggcacctac
      901 acgtccaagg atcggatgat cgagcgtctc aacgagggag cagacgaggc cactgccgag
      961 gtttctctcg ctgagatcaa gtctgaaggc gagtcaggcg aacccgccta gagctttccg
     1021 aaaaacttcg acgatctggg ctccgttctc ctggttgcga tccttctccg ggttgtagca
     1081 acccagcgaa atcccgacca gtgcaggtga tgagcagacc gcggacagga gctccgtcaa
     1141 ctctgtccag tcgaggccgt tgggcaccgg tgcgtcgttg gcgaagaaca ggtatggatc
     1201 cacgatgtcg aggtcgaggt ggacccagat gttgcccacc ccattggcga gagcgcccgc
     1261 cgtccgacgc ccgacgtcac cggagccgag agagcgcatc tcgtccgtgg tcaggcatgc
     1321 gatcgggggg tccacatcct cgggcatgat catcccatcg acctccgact gctcgcggtc
     1381 gcggtatccc aagatccaga cgtctccgcc cgacaggctc ggaccgcctg ccgcctggac
     1441 ccaggccgca ggtcccagcc caagggcgac cgtgaccggc atgtctgccg cctcacccgt
     1501 gggggaggtc gtcgagtcat agagatcgag atgcccgtcg agatgaacga ggcttgctgc
     1561 gcctcgggcg tcgcgcactc cggccagggc cccgggcacg atcgaacagc agccgccgac
     1621 gagaaagggg cgctcctcct tggcaagggt ctcggccacg gccctccgaa cctcggcggt
     1681 cgtggcgcac acatcgtcga gagcgacaac cccggtgacc gggtcgcgcc cctccgccct
     1741 gattcgaacc tcgagatcgc cctcgtcgcg gccgtggatc gcctctccga gcccgagcga
     1801 gcgaagcgca gcgggtgcca gctcagtccc gccgggacca cccgccgagt caacaggtac
     1861 tccgatcatg gcgaatccgg acttcatggg cccaaactat ctacagattg gatgtgatcg
     1921 ccgcctgccc actcacaacg taggattgtc ggatggccga cagagaggtg tagctcagat
     1981 ggagggggta ggggcagcag taatcgccat agtggttctg gcggttgtgg tcgtcagctt
     2041 gcgcaggcag cgcaagaggg ccgctgcagt cgatctcgaa gacacctcgt ggctgaggcg
     2101 tgctccggat gagccggaac cgctaaccaa gcgcctggcc gtcggcgagt ttcatgtatc
     2161 cggacatgag gcgcgggtgg tcttcgatgt cccactacct gaagaggaag atccgattct
     2221 caacgagctg ctcgtcgacg aggcagtcga ggtggtccgg gagaagcgcc attctttgcc
     2281 catcgatgac gtgaccgaga tcgtcgtctt tgcggggcga ggcaactcga gagagatcgg
     2341 cagaacgacc cttccggctc cgggtcagct accggtggct atccagcccg acatgctgaa
     2401 tctgacccac atcgcccgcg atccgttcgc gagtcagttc gaagacgacc cgatggcgca
     2461 ggtcgagacg cgggtggacg tacccgcgga cgatctcggg ccgatcagga atgaattgcg
     2521 gatcccgttg ggcttggagc gcggaattcg gactcgtgga gtcgacccgg ccaccgcttc
     2581 gggccccgag ctgatcctcg ccttgctcga aatgttcgga tacgaggtga gcccacaggt
     2641 cgacgggacg ttcatcgcga ccaaggacaa ggtgaagact ttcatacgcg ccgagcccca
     2701 cgactcagga tcgcatcccg agctggacga gcaggtggtg cagaagttca tcgtcgagtt
     2761 cgggacctcg ggcgccacac gagggctgct gatcagcgac aagctcggcc cgttcatgat
     2821 ccacgatgtc gaagcgatga atccccgggt tcgattcgtg acccggcagc ggattcagcg
     2881 tttcatcgac tccatggcca tcggctgacc atcgcggggc aatatcgggc tgggggctaa
     2941 gctgcgcggg taaggaggtc ctattgctcg gtctcagagg ccaagaatgg ctgataattc
     3001 tgctgatcgt gctcctcatc tttggagccc gaaagctgcc tgacctcgcc cgttcgcttg
     3061 gtgcttcggc caaggagttc cgcaagggca tcgacgaagg aaccgagacg gaatcggaca
     3121 cgcagacccc ggaaccgcca cctccctcgg attcctagga accggacgcc cttctcgggc
     3181 gttgatggag taggtactcc atgacggagt ccgtcccact cacctgggaa gaggtcgcgc
     3241 gtgtctatgg caggaagatc tacaacttcg cctaccggct caccggtaat ccagacgacg
     3301 cggccgatct cgtgcaagaa gtcctgcttc gtgtccgtcg cggcctcgcc tcctaccagc
     3361 cggggagctt cgagggctgg ttgtggcgga tcacgcggaa cgcatttctc gacgacgttc
     3421 gcaagcgcaa tcgtcggcca attgcgccac tccccgatga gatcgaccgg tgggagctgg
     3481 cttccacgcc gggttcggac gtcgagtact cgcggatcag cctgggtgac gacatccaaa
     3541 aagctttgct ggaggtccca atcgagttcc gagaggcagt cgtcctctgc gacgtcatcg
     3601 ggctctccta cgaggagatt gcaatcgcag tctcagttcc gatcggaacg gtccgcagcc
     3661 ggatccaccg cggtcgcaag atcctcaagg acctcctgag ttgacgcacc ccaccgagct
     3721 gatcagcgcc tatctggatg gtgagttgac ccgcgccgag gtctcccagc ttcaggccca
     3781 catgagctcg tgcgggaagt gcgtcgccga catggaggga ttgcagaaag tcaggacctc
     3841 cgtccgctcg ctgcccttga tcgagcttcc acccgggctg cttggggatg ccgagccggt
     3901 cgttgtccca ctgcgaagga accgtggctt ttgggttggc gctgccgctg ccggggttgc
     3961 ggtggtgatc gcggtagcaa ccatgctcac ccctcagccc acctcgttgt cagtcaatga
     4021 cctgaacagc cgattcgggg cgagggtctc actggatccc gcctttggcc cggcgaaggt
     4081 ggctgtccct gacgtcaggg acttgaccga atgaggcgtg ccgttgtggt aggcgttctc
     4141 ctgacgctgc tccctgccac cgcggcgtcc gccgccgacc tcgaagagtt gctggatcgg
     4201 agtcacgacg cctcctactc cgccgagcag gtgatcagtt gcaacacccc ggagggcgct
     4261 cgcgatgccg tggtttctct ggagcaggtt ggtggcgagc tgcaggtgtc cggcgaagtc
     4321 gaggtgtctt cgggctctgg cggatgggcg ctgacgagcg agggctctgt ggtcgacagc
     4381 gcttcggttg aggccgggac caacggtgcc gagcccatct accgagtcga tgacggagtg
     4441 gccaacccat ttctcgatcg caaggccggc ctgtacaggc tttacgccgg tgacgtcctt
     4501 cgtggcgagc tcatcgtcga tggcgccacc ggagctctac tcagcgtcgt cacctacaac
     4561 gccgacggtg aggtctactg cgaccgtcga ttcatcgaat tcgatccgac tgctcccgac
     4621 gtcgcggttc cgtcagtagc gagtggcgag gccctcgagc ccagtgagga tgcggcggcc
     4681 ttccccgaac ggctcggcac cttcgagcgt ctcgacattt atgaggatgg tgagggattg
     4741 gtcttcgcct attactcgga cgggttcttc tcatttgccg tgttcaagac tccgtcagtc
     4801 gtcgagctga ccgatccagg ttcgctgact ctcgaaggcc tggtctatgg gagatcgttc
     4861 tctcccggac aggtgacata cagctgggaa accaggaacg gggggatggc tctcgtgggt
     4921 gaccttccgc cggacttgca ccccgagatt ttggcgggcc tcgatcagcc cttctcgccg
     4981 ggcattctgg gaaggctctg gcgcaacctt ttcggctgat caggcgtcgt cgtcggcgga
     5041 gcgatgctcc acgatccagg cttcggcgtc ttggagggca gccagtgtgg actctccgat
     5101 gttcggcgtt gcttcgtaca attggatcgg cccttgcgct gagacatgtc ccgctcgcat
     5161 gaaatgactc atcacccgag gctcgatccc gctcaggtag aggcgaccct cggccgcctc
     5221 gagccggcct gcgtagtcat cgaggacttt gaaagcggtc gcccccaatt cgatcctgcc
     5281 tcgcatcctg agaatcacga cgggctcgct ggcaccgcca atctcgggga ggcgggcttg
     5341 aagggttcgg gcaccggcgt agaaaaggct cccgtaggcg ttgaggatgg tcacgtgttg
     5401 gctctccagc gaacgaggag ccggctgctc cgcaaagctg ttctccggcc ccgggaccca
     5461 ctccacaacc cggaggtcta tcgcctcctg attcagctgg agcatgagcg atagcaccac
     5521 accgaaggcg acggcggccg caattgggag aaacaaggtg gcgaagaagg ttgagatcag
     5581 ggctatctgc gagttgggac cggtgcgaag gatgctctcg atctggccga ttcggagcga
     5641 cccgatggcg gccacgatca ggatcgcagc gagcgtgggc atcgctacca ccccgaccac
     5701 acctgagaag acaaccaata tcacaagcat ccagatcccg gatgagatcg aggcccaacg
     5761 gtccctcgct ccggcactga tgttgagggc ggtctggcct acggagccgc ccaccgccag
     5821 cccgcggaag aaccccgacg caaggttgcc gaccccttgg gcgatgaaat cgcggtcggg
     5881 gttggaggtc gagccgtcac gattgggcgc tgactcggcc actcctgcgc cttggaccag
     5941 gacgatggct gcgatggcca acgccgcgac gaccagggac ggcgtcaaga gggtgatgtc
     6001 gggcaacgct gggatgggca ggccttgtgg gatatcgcca acatcttcca cccgggcgac
     6061 gctttcgact cccagcacca ggatggtcgg cacaacgagg gcgataatcg acgcgtatga
     6121 gctcagcgga gtgcgcccca ggatcacgat cagtgccacc gcggcgagac cggtcagtag
     6181 cgacaccgga tcgattgacg acggatgaag cagcacgttc agagccttgg ccagagagtt
     6241 cgcccctacg gattcggctc cggtgaagtc tgggatctgg ccgaagatga tgttggccgc
     6301 gactccggtg aggaacccga tcatcaccga gtgggacacg aacctggtgt agcgaccgag
     6361 cttcaggact ccggcgagga tcatgatggc ccctgccatg aggaccagca ggaacagaga
     6421 ggccgcctga tcctctggat cgattgagct cagggcagac ccggcggcga gggcggcggc
     6481 cgtcgtggtg gtgataacca tcagacgagt gctcgctgtc agaccgccgc cgatcgggcc
     6541 ggcgaagctg gcatagaggc catagacagg gttgaccccg acgagaaccg cagaggccat
     6601 gccatcggga acactcccga tggcgcccgg aagcccggcg atggcgtctt gtttcaaggt
     6661 tcctggtctg gggcggaccg aggcccacca ctgcgacacg acattcatgt cgaagccccg
     6721 acagcgccat ccggggtggg ctcttgaccc tccctcggga tgcgtctcgc cttgaggagc
     6781 aggatcacgc tcaggatcag gatccagatc ggaaacacca acaccaacag tctgttgagt
     6841 gttgcgacca gaaggaggaa gaggcccacc acgtagccag cccagacaaa ccaccggggc
     6901 aggaccttgt ggcgtcgggc aatagctgag gtggtgaaga caaacatggc tgccatccgc
     6961 atcgagaaca ccagtaggag tgcggagccg aattgcggga attggcgggc cgcccccggg
     7021 tcgaccggat agtcggagaa ttcgacgctt actgcaagga ccgtggaggc cgcggcaccg
     7081 gcaaggaaga gagcgatgaa gatgatggca ctgaccagtt gcacgttgga aagcaggaca
     7141 tcctgagtcg atgtcatgcg ggagagccac atccgcagtg ccaccgcgaa ccaaaggaag
     7201 gcgatccctg caaacggcat taggtacaac ccaaccaaga ccaccaactg
//
LOCUS       DBNHXU010000050         1869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4381074, whole genome shotgun sequence.
ACCESSION   DBNHXU010000050 DBNHXU010000000
VERSION     DBNHXU010000050.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1869
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4381074"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..713)
                     /locus_tag="AC161X_01340"
     CDS             complement(15..713)
                     /locus_tag="AC161X_01340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013769933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HIR4988223.1"
                     /translation="MATRTERRSRPRDPLTHERVMRAAVDLADRSGIEALTMRNLAHE
                     LGVEAMSLYYHVANKEAVLDGVAEVVLSEIEAAVDRIDPPSHPDGWKSAMRARILAAR
                     EVLLRHRWAPSVLETRTAISPTTMRYFDSLLGLFRFGGFSYDIAHHALHALGSRALGF
                     TQELFEPDNVDDGEQDATIAIEDMADQFPHLVGMLAEISHDDPDSTLGWCDDQTEFEF
                     GVDLILDGLDRLRV"
     gene            827..1798
                     /locus_tag="AC161X_01345"
     CDS             827..1798
                     /locus_tag="AC161X_01345"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020630545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR4988224.1"
                     /translation="MKAIVQDRYGSPDVLELRDIDQPVPGDDEVLIQVRAAGVDPGVW
                     HLMTGLPYLVRVMGYGLAKPKNGVRGRDVAGRVEAVGKHVTRFRPGDDVFGTCEGSFA
                     EFATARQDRLALKPAIVSFEEASAVPISGITALQAVRDKGRVGPGQKVLIIGAGGGVG
                     TYAVQIAKALGAEVTGVCSTSKLELVRSLGADHVIDYTLEDFAEGSIRYDVIIDNAGN
                     RSLTRVRSSLTRKGILVIVGGEEGGQWVGPANRLLPAILLSPFVTQELKVLMATEGKE
                     DLEVLAEMIETGKVRPIVGRTYPLIEAPEAVRYVGEGHALGKVVVTV"
BASE COUNT          350 a          533 c          611 g          375 t
ORIGIN      
        1 gggggttggc cggtctagac gcgaagccgg tccaggccgt cgagtatcaa gtccactccg
       61 aactcgaact cggtctggtc gtcacaccac cccaatgtcg agtcggggtc atcgtggctg
      121 atctccgcca gcattccgac gaggtggggg aactggtcgg ccatatcctc gattgcgatt
      181 gttgcgtcct gttcgccatc gtcgacattg tccggctcga acagctcctg ggtgaatccg
      241 agtgcacggc tgccgagtgc atgcaacgcg tgatgagcaa tgtcatatga gaatccgccg
      301 aaccgaaaca gcccgagcag gctgtcgaag taacgcatcg tggtcgggct gatcgcggtc
      361 cgcgtctcga gcacgctcgg cgcccatcgg tggcggagca ggacttcccg ggccgccaga
      421 attctcgccc gcatcgccga cttccatccg tccggatggg aaggtgggtc gatccggtct
      481 accgccgctt cgatttcgga aaggacgacc tctgccactc cgtccaggac agcctccttg
      541 ttggcgacgt ggtaatagag cgacatggcc tcgactccga gctcatgggc gagattgcgc
      601 atcgtgagag cctcgattcc gcttcgatcg gcaagatcga ctgcggcacg catcacccgc
      661 tcgtgggtca gggggtcgcg agggcgtgat cttcgctcgg ttcgggttgc catgtctcca
      721 tcctaaccgt acagggtaag tctgatattt ccacttgaca gccttacgtc gtacggatta
      781 ccttacgcca tacgacttac aacgtacgag agatgaggag gacattgtga aggcgatcgt
      841 tcaagatagg tacggctcac ccgacgtctt ggagctgagg gacatcgacc aacccgttcc
      901 tggcgacgat gaggtgctca tacaggttcg tgcggcgggg gttgatcctg gggtatggca
      961 tctgatgacc ggcctcccct atctggtgcg cgtgatgggc tacggattgg caaagcccaa
     1021 gaacggtgtc cgtggccggg acgtcgccgg gcgggtcgag gcagttggca agcatgtgac
     1081 ccggttccga ccgggcgatg atgtgttcgg gacctgtgag ggctcgttcg ccgagtttgc
     1141 aacggctcga caggaccgac tggccctcaa gcctgccatt gtgagcttcg aggaggcatc
     1201 cgcggttccg atctccggca ttacggcact gcaggcggtt cgcgacaaag gccgtgttgg
     1261 tccggggcag aaggttctca tcatcggggc cgggggaggg gttggcacct atgcggtgca
     1321 gatcgccaag gcgcttggag cagaggtcac cggggtctgc agcacatcga agctcgaatt
     1381 ggtgcggtcg ctcggcgccg accatgtcat tgactacacc ctcgaggact tcgcggaggg
     1441 gtcgatccgc tacgacgtga tcatcgacaa cgccggcaat cgctcgctga cccgagtccg
     1501 gtcttcactc acccgcaaag ggattctcgt catcgttgga ggtgaggaag gaggacaatg
     1561 ggtagggccc gccaatcgtc tccttccggc aatcctgctc tcaccgttcg tgacccagga
     1621 actgaaggtg ctgatggcca cggagggcaa ggaggatctc gaagtgctgg ccgagatgat
     1681 cgaaaccgga aaggtcaggc cgattgttgg ccggacatac ccgctgatcg aggcgcctga
     1741 agcagttcgt tatgtgggag aggggcacgc gcttggcaaa gtcgtcgtca ccgtctgaac
     1801 gagacctatt ggctggggca tcatgaaggc gatcgtccga gacgagtacg gctctcccgg
     1861 tgttcttcg
//
LOCUS       DBNHXU010000051         1750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2793873, whole genome shotgun sequence.
ACCESSION   DBNHXU010000051 DBNHXU010000000
VERSION     DBNHXU010000051.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1750
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2793873"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1276)
                     /gene="hutH"
                     /locus_tag="AC161X_01350"
     CDS             complement(<1..1276)
                     /gene="hutH"
                     /locus_tag="AC161X_01350"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF006871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004397 - histidine ammonia-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - L-histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HIR4988225.1"
                     /translation="MSSIVIDGNPLTRADVIAVARDGVTVATGHNLAGRMKPSRDVVE
                     AAVDSSEVVYGITTGFGALASTHIGRDQTEALQYNLVRSHAAGVGDPLPDTVVRAMLL
                     LRARTLSQGHSGVRPEIVERLIEMLNRGILPLVPSQGSVGASGDLAPFAHLALPLIGE
                     GEVRWGGEILTGAEALTRAGLEPLRLHTKEGLSLLNGTEGMAAMGVLAHDQANTLLLA
                     ADVACALTVEAVMGSSRPFRPEVHGLRPHPGQSRSARRIAALLEGSGIGASHADDFHH
                     AVQDAYSLRCAPQVHGAVADTLNHMAGVLDIEIGSVVDNPIVFPDTAEVISAGNFHGQ
                     PLAFVLDFCAIAVTELGSISERRTDRMLDPTRSAGLPAFLSPDPGVNSGYMISQYTQA
                     ALVAENRVLSHPASVESIPTSGMQEDHVSMGWG"
     gene            1342..1749
                     /locus_tag="AC161X_01355"
     CDS             1342..1749
                     /locus_tag="AC161X_01355"
                     /inference="COORDINATES: protein motif:HMM:NF024773.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR4988226.1"
                     /translation="MTDLTPLLTQPGASVAVIGATDHPSKYGGIIYRDLKTKGYKVFA
                     VNPYRETVDGDPCWHSVLDLPENPTIADFVIPAARGLLVLDECEKAGIQNIWIQPGAE
                     SPELEKRLASGPFVWLTRACIMVRAGYAAALGG"
BASE COUNT          320 a          544 c          595 g          291 t
ORIGIN      
        1 cgccccaacc catcgagacg tggtcttcct gcatcccgga ggtgggaatc gactccaccg
       61 aggcgggatg agagagaacc cggttctcgg cgaccagggc cgcctgggtg tactggctga
      121 tcatgtaacc ggagttcacg ccgggatccg gcgacaggaa ggcagggagt ccggccgagc
      181 gggtcgggtc cagcatgcgg tcggtccgcc gctccgagat cgaacccagt tcggtgaccg
      241 caatcgcaca gaagtcgagg acaaaggcga ggggctggcc gtggaagttg cccgccgaga
      301 tcacttcggc tgtgtcgggg aagacgatcg gattgtcgac cacggacccg atctcgatgt
      361 cgaggactcc tgccatgtgg ttgagcgtgt cggccaccgc gccgtgcacc tgaggggcac
      421 agcgcaatga gtacgcgtct tgaaccgcgt ggtggaagtc gtcggcgtgg ctagccccga
      481 tcccactgcc ttcgaggagg gcggcaatcc gacgagctga tcggctctgc ccggggtgag
      541 gccgcaggcc gtggacctcg ggccggaacg ggcgagaact gcccatcacc gcctcaacgg
      601 tgagggcgca ggccacgtcc gcagccagca ggagggtgtt ggcttgatca tgggcgagga
      661 cccccatcgc ggccattccc tcggtcccgt tcaacagtga caggccttcc ttggtgtgaa
      721 ggcgcagcgg ttcgaggcct gccctcgtta gggcttctgc gccggtgagg atctcgccgc
      781 cccaacggac ctcgccctca ccgatcaagg ggagcgcgag gtgggcgaaa ggtgccaggt
      841 ccccggaggc accgaccgag ccctgggagg ggacaagggg cagaatgccc cggttgagca
      901 tctcgatcag gcgctcgacg atctccggcc gaaccccgga atggccctgg gacagggtcc
      961 gcgctcggag cagaagcatc gcccggacca cggtgtcggg aagcgggtct cccacgcctg
     1021 cggcgtggga gcggacgagg ttgtattgaa gggcctccgt ctgatcacgg ccgatatggg
     1081 tggatgccag ggccccgaat ccggtggtga tcccgtagac aacctcgctc gagtcgacgg
     1141 cagcctcgac gacgtccctt gaaggtttca tgcggccggc caggttgtgg cccgttgcca
     1201 cggtgacccc gtcgcgggcg acggcgatca catcggctcg ggtcagggga ttgccgtcga
     1261 tgacgatgct gctcacgctc actcacggta catattggat tccagggaag cccataactt
     1321 ccgataccgt tgtctatgga aatgacggat ctcacaccac tcctgaccca gcccggcgct
     1381 tcggttgcag tgatcggcgc caccgatcat ccctcgaagt acggcgggat catctaccgg
     1441 gatctaaaaa ccaagggtta caaggtgttt gccgtcaatc cgtaccgaga gaccgtcgat
     1501 ggcgacccct gctggcactc ggtcctcgac ctgccagaaa acccaaccat cgccgatttc
     1561 gtgattccgg cggcgcgggg cctgctcgtg ctcgacgaat gtgagaaggc cggaatccaa
     1621 aatatctgga tccagcccgg ggcggagtca ccagagttgg agaaacgact tgccagcggc
     1681 ccgttcgtct ggctgacccg agcctgcatc atggtgcggg ccgggtacgc ggccgctctg
     1741 ggaggctaga
//
LOCUS       DBNHXU010000052         9418 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5175597, whole genome shotgun sequence.
ACCESSION   DBNHXU010000052 DBNHXU010000000
VERSION     DBNHXU010000052.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9418)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9418)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9418
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5175597"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..193)
                     /locus_tag="AC161X_01360"
     CDS             complement(<1..193)
                     /locus_tag="AC161X_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017236540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HIR4988227.1"
                     /translation="MSQEVPAERTFPDGFRWGTATAAFQIEGAVNEDGRGTSIWDTFV
                     RTSDQMLVDDNADVADDHYH"
     gene            286..3027
                     /locus_tag="AC161X_01365"
     CDS             286..3027
                     /locus_tag="AC161X_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011022472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HIR4988228.1"
                     /translation="MTLQVSRETNWYGSTAAEVAAALEVSPEVGLTQAEVGSRLERYG
                     SNRLTGAKKESGFQAFLRQYQDFMQIILLAAAVMNQLVTGDTATTVVLAGLTLFNAVI
                     GMRQESKAEESVAALASMLKTIARVRRDGQVLEVQAEELVPGDIVLMEAGNLVPADGR
                     IMVAATLEIEEAALTGESLPVGKSIDPVPGDEVALGDRICMGYMNTSVTRGRGEMIVT
                     GTGMSTEVGHIADLLANTGTDKTPLQKQLDALSKIIATIAAAALVIVVLLGLSAGQSF
                     DTLFITGVALAVAAIPTGLPAVVTALLSIGTREIARRNAIVKRLPAVETLGSTSAICS
                     DKTGTLTLNKMTAREMAIPGENVFRVSGEGYSTAGEIKHVGGENFDLDPYLLPMVLCA
                     DAVLDGESLIGDPTEGALIVLGAKGGLDIDLTRSTYPRVAEVPFDSDYKFMATFHEME
                     SQEGSAVIRCYVKGAPDVLIAKGGSYLSPDGKKVPVTDENRHLALDVNERMAGSGERV
                     MVVAQRDFDPGSFDPRGNLIDLVRDLTLLAMVGIVDPPRAEAKAAIAKCHDAGIQVRM
                     ITGDHAVTAAAIAGELGIEGRAITGAQFAAMTDGELIEQLPEIGVIARVAPEDKVRLV
                     RMLQQSGNVVAMTGDGVNDAPALKAADIGVAMGITGTEVSKEAAVMILTDDNFATIVG
                     AVEYGRSLYDNLLKYLRFQMSTLVAYIAVFIGAGIFAVAGGIPLNPLQILWLNMVIDI
                     PLAIALGFDEPTPGLMKRSPRPVGAPVLSSRNWVRLCVQGFVMTVGTLAVYQITEPEY
                     GALVASTMLLTTLSLFHLIAGYMARDQENTIFDRASLPGSTQLRRYGIALFAIVAVTG
                     LGFLQRIFGTVELSFQQWATCIGISLSLLVVEELIKFFIRRRGRAPEAVPTSPALAV"
     gene            complement(3048..4550)
                     /locus_tag="AC161X_01370"
     CDS             complement(3048..4550)
                     /locus_tag="AC161X_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016492941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR4988229.1"
                     /translation="MATIVPRWEWRTFGASFPTAEDYFSALDPIEAKTSDDLYLVGEV
                     AANVKIRDDLVDVKVLKEVDSRGLELWTPMMKVGSPIPANDVLEVFAVLERPAPDLAR
                     DQYTMSQFLEEVVAADRVLQAVQVHKTRVRYQLDDCIAEVTDLDVGDRTVRTIAIEGE
                     HPDSVISAVDAAGLGEYLNVSYTTGLRSLIAGAGPRYAVIDIGTNSVKFHLGELQRDG
                     QWAAVVDRAELTRLGEGLEEHQEFQPSAIARTVDAVIGMVEEAKDSGARAIAAVGTAG
                     LRIAPNRDLVTGPIRARTGVKVEVISGEEEGRLAYLAVEAGLGLGTGSLVVFDTGGGS
                     SQFTFGEGAQVIERFSVQVGAVRYTETFALDHEVDLETVTAALAAISADLDRLNDRAT
                     PNALVGMGGAITNITAVSLELAAYDPDLVQGATLDIAEIDRQIDLYRGMNAEARRSIV
                     GLQPKRAEVILAGACIVRTIMEKLGQTALTVSDRGLRHGLLIEKFGPVSR"
     gene            complement(4563..6188)
                     /locus_tag="AC161X_01375"
     CDS             complement(4563..6188)
                     /locus_tag="AC161X_01375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4988230.1"
                     /translation="MAGAGLVLLTLSTGQFLMTLDSAVMNVSIAQVAEDVGTTVTGVQ
                     TAITLYTLVMATMMITGGKIGSMIGRRKAFAIGCIIYGAGSLTTALAPTLTVLIIGWS
                     FLEGLGAALIMPAIVALVASNFPPSGRARAYGLVASAGAIAVAAGPLIGGAVTTYWTW
                     RYVFAGEVVLVIAILALSRKMHDVPLEPQPPLDLVGVALSAIGLGLAVFGVLRSGTWG
                     WVQPKPDAPQWLGLSMTVWLILAGSFIVWLFFAWEERLIGRGREPLVRPQMLQNRQLA
                     GGLIMFFFQFLIQAGLFFIVPLFLSVALGLSALDTGIRLLPLSVTLLIAAVGIPKYRP
                     QANPRTVVRWGLIFLFFGIVSMVGALEIGAGAEIVTIPMLLAGVGVGALASQLGAVTV
                     SGVRDEETGEVGGLQNTMTNLGASLGTALAGSILIAGLTAAFLEGVVANPDIPPEVSN
                     QAEVELASGIPFISDAQLETALAETGLPPDQVNAIIDDNEAARIAGLRASLSVLAVLA
                     LSGLFFTNRIPTVPVGHSPPAADPDGVNAAETA"
     gene            complement(6230..7432)
                     /locus_tag="AC161X_01380"
     CDS             complement(6230..7432)
                     /locus_tag="AC161X_01380"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR4988231.1"
                     /translation="MTDAGNQPPAQQGQIAPEEAELVPISGQAWSASTRRLVAIALVI
                     GVVALGFFLLFGVLDSIIVAALLAFLIEPILRGLVNKLHFPRWLAVTVVYLATAAIAI
                     GAVFILPALIIGSLAQIDFHEIVASFETWLTNVATSLSSTNFLGVDLTAVSAVSDASS
                     QASTSADLLDPGRWLGALTQALVAAAGAAGLLIGIISGIVFVLIIAIYMTADSSRYFR
                     SIANLVPKEYELEVFELGRRLNASWNDYIRGQGIMILVIGFTTFVVVWLIGLPGALFL
                     AVIAGLLEVIPTFGPIIATIPAVIIALVQGSTRFVDMNNIVFALLVIIAYTLIQQLES
                     NIVAPKVMGHSVQLPALVVLISVTAGYQVAGILGAILAVPVVANVRVLLSYLWAKIHS
                     REPWAEPT"
     gene            complement(7475..8890)
                     /locus_tag="AC161X_01385"
     CDS             complement(7475..8890)
                     /locus_tag="AC161X_01385"
                     /inference="COORDINATES: protein motif:HMM:NF014522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding protein"
                     /protein_id="HIR4988232.1"
                     /translation="MRSRFAIIALTVVLGLTISGCTEEEPIDVAAAQAQFCSDVEQDV
                     SALGSYGGLFEDVDLTVGDVKNAADELAPGREAVEESAATFQEAVTADTTPGLTVEII
                     QPESIDAVKDAEAAFAAASDIDDNTTVVEAGVNFTSAAYGLEVAWVRLFADAGCIEDE
                     AQASQWVSDYVTALQTDLQAAGLYDGAIDGIYGPQTIAAVEQLQKDAGLPVTGLVDPA
                     TQAALAAALGQRQSAEIGALQGILISTGYYDGPVDGVWSRELEDALKALQTDLGVPAT
                     GVVDAATLRAFQKALNDAGEAPPTTATTEPDRGPATTLPAETTTTAAAATTTVAPPDT
                     TTPQPSGGILEVLADAGEFSQLLAAIDAAGLTETLSGPGPFTLFAPTDDAFAELAEPL
                     PSDPEALAAILLYHVVNENLGAFDLMDMSSVTTAQGSDIAITVDNDQLVLNGVSTVTV
                     SNVIGSNGTAHVVNTVLTPPG"
     gene            9057..>9418
                     /locus_tag="AC161X_01390"
     CDS             9057..>9418
                     /locus_tag="AC161X_01390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018101720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR4988233.1"
                     /translation="MDLFWTMLWLFLFFAWIWLLITVFSDIFRSDDLGGWGKALWILF
                     TVFLPYLGVLVYLIARGGSMQQRTLAEHAAREEMTRAYVQQVARSGSTAEELTQLANL
                     RDTGVITAEEFNQQKAKLL"
BASE COUNT         1806 a         2878 c         3009 g         1725 t
ORIGIN      
        1 ggtgatagtg gtcgtcggct acgtccgcgt tgtcgtcgac cagcatctgg tccgatgtcc
       61 gcacgaaggt gtcccagatc gaggtgccac gaccgtcctc gttgacggcg ccctctattt
      121 ggaaggcggc tgtggcagtg ccccatcgga acccgtctgg gaatgtgcgc tcggcgggaa
      181 cctcctggga catggtcacg accggcgaga gggtagagaa atggcggagc atggctcgaa
      241 acgaagcggc acctaatagt gtccggaaaa agaaaggctc attgagtgac acttcaggtc
      301 tcgagggaaa ctaactggta tgggtcgacg gcggcggagg tggcggcggc gctcgaggtc
      361 agccccgaag tcggtctgac tcaggccgaa gtaggcagcc gcctcgaaag gtacggttcg
      421 aaccggctga ccggggcaaa gaaggaatct ggtttccagg cctttcttcg ccagtaccag
      481 gacttcatgc agatcatcct ccttgccgca gctgtcatga accagctggt gacgggagac
      541 accgctacga ccgtggtctt ggctgggctc acacttttca atgctgtcat cggcatgcgc
      601 caggagtcga aggccgaaga gagcgtcgcc gctctcgcct cgatgctgaa gacgattgct
      661 cgagtccggc gcgatggcca ggtgctcgaa gtgcaggccg aagaactggt gcccggggac
      721 attgtgttga tggaggccgg caacctcgtc ccagcggatg gccggatcat ggttgctgca
      781 accttggaaa tcgaggaagc cgccctgacc ggtgagagcc tgcccgtggg gaagagcatc
      841 gacccggtgc caggagacga ggtggcactc ggcgaccgca tttgcatggg ctatatgaat
      901 acctctgtca ccaggggtcg tggcgagatg attgtcaccg gcaccgggat gagcaccgaa
      961 gtcggccata tcgccgactt gctcgccaac accggcacgg acaagacgcc gctccagaag
     1021 caactcgacg cactctccaa gatcatcgcc accatcgctg ccgcggccct ggtgatcgtt
     1081 gtattgctcg gactttccgc gggccagtca ttcgacacgc tcttcatcac cggcgttgca
     1141 ctggccgtgg cggccatccc gaccggtttg cccgccgtcg tgaccgcctt gctttcgatt
     1201 gggacccgtg agatcgcccg tcggaacgca atcgtcaagc gcctccccgc agtcgagaca
     1261 ttgggatcga cgtcggcaat ctgctctgac aagacaggga ctctgacctt gaacaagatg
     1321 accgcccggg aaatggcgat tccgggcgag aacgtcttca gggtgtcggg tgagggctat
     1381 tcgaccgccg gcgagatcaa gcatgttggt ggcgagaact tcgatctcga cccgtacctg
     1441 ctcccgatgg tcctctgcgc cgatgcggtt ctcgacgggg aaagcctgat cggagatccc
     1501 accgaggggg ctctgatcgt gctcggcgcc aaggggggat tggacatcga cctcacccga
     1561 tcgacatacc ccagggtcgc ggaggtcccc ttcgactcgg actacaagtt catggccacc
     1621 tttcacgaga tggagagcca agaaggttcc gcagtgatcc gttgctatgt caaaggcgcc
     1681 cccgacgttc tcatcgccaa gggtggctca tatctcagcc ctgacgggaa gaaggtgccc
     1741 gtcaccgacg agaaccgcca tctcgctctg gacgtcaacg aacgaatggc cggctcgggt
     1801 gaacgggtca tggtggtggc gcaacgagat ttcgacccgg gttcgttcga cccgagaggg
     1861 aatctgatcg atctggttcg cgatctcacc ttgctcgcca tggtggggat cgtcgacccg
     1921 ccccgagccg aggccaaggc tgccatcgcc aagtgtcacg acgctgggat ccaggtacgg
     1981 atgatcacgg gcgatcacgc cgtcaccgca gccgccatcg ccggggagct cggcatcgaa
     2041 ggtcgtgcga tcacgggtgc tcagttcgcc gcgatgactg atggggagct gatcgaacaa
     2101 ctaccagaga tcggcgtgat cgcccgcgtt gcccccgagg acaaagtgcg cctggtccgg
     2161 atgctgcagc aatccggcaa cgtggttgcg atgaccggtg atggggtcaa cgacgctccc
     2221 gccctcaagg cggcggacat cggggtggcg atggggatca ccggaaccga ggtctccaag
     2281 gaagctgcgg tgatgatcct caccgacgac aacttcgcca ccatcgtcgg cgcagtcgag
     2341 tacgggcgtt ccctctatga caacctgctc aagtatctcc gcttccagat gtcgacgttg
     2401 gtcgcctaca tcgcggtgtt catcggcgcc gggatatttg ccgttgccgg tgggattcct
     2461 ttgaacccac ttcagatctt gtggctcaac atggtcatcg acattccgct ggcgatcgcc
     2521 ctcggattcg acgagccgac gcccgggctg atgaagcgtt ctccgcgccc tgtcggagca
     2581 cccgtcctct cgtcgcgaaa ttgggttcgg ctgtgcgtac agggcttcgt gatgaccgta
     2641 ggcacactcg ctgtctatca gataacagag ccggaatacg gcgcgttggt tgcgtcgacg
     2701 atgctgctca ccacgctctc gctgttccac ctcatcgccg gctacatggc cagggaccag
     2761 gagaacacga tcttcgatag ggcgagcctt cccgggtcga ctcaactccg tcgctacgga
     2821 atagctcttt tcgccatcgt cgcggtcacc ggcctcgggt tcttacagcg gatctttggg
     2881 accgtggagc tcagcttcca gcagtgggcg acatgtatcg ggatctcgtt gagcctcctc
     2941 gtggtcgagg agctgatcaa gttcttcatt cggaggcgag ggagagcacc ggaagccgtt
     3001 ccgacctcac cggccctggc cgtttagagg gtggaatccg gttcccctca cctcgagacg
     3061 gggccgaact tctcgatcag gaggccgtgc cgcagcccgc gatcgctcac agtgagcgcc
     3121 gtctgaccca gtttttccat gatggtccgg acgatgcagg ccccggccag gatgacctca
     3181 gctcgtttcg gctgcaggcc gacaattgag cgtcgtgcct ctgcgttcat gccgcgatac
     3241 aggtcgatct gacgatcgat ctcggctatg tccagagtcg caccctggac caggtcaggg
     3301 tcgtatgccg ccaactcgag gctgacggcc gtgatgttgg tgatcgcccc gcccatgccg
     3361 acgagggcgt tcggcgtggc acggtcgttg aggcgatcca ggtcggcgga gatcgcagcc
     3421 aaggccgcgg taactgtctc gagatccacc tcgtgatcga gtgcgaaggt ctcggtgtag
     3481 cggacggcgc ccacctggac gctgaatcgc tcgatgacct gcgctccctc gccgaaggtg
     3541 aactggctgc tgccgccacc ggtgtcgaag accaccaggg agcctgtgcc gagccctagc
     3601 cccgcctcga cggccaggta ggcgagacgg ccctcctcct cgcccgagat gacctccact
     3661 ttgactccgg tcctcgcacg aatgggccca gtcactagat cgcggttggg ggctatgcgg
     3721 agcccggcgg tccccactgc agcgatcgcc cttgccccgc tgtctttcgc ctcctccacc
     3781 atcccaatta cggcgtcgac ggtgcgagca attgccgagg gctgaaactc ttgatgctct
     3841 tccaacccct ctccgaggcg agtgagctca gcccgatcga cgacggcggc ccactgcccg
     3901 tcccgttgca gttcgccgag atggaacttc actgaattgg tgccgatgtc gatcaccgcg
     3961 tagcgcgggc cggcaccggc gatcagcgac ctcaggccgg tcgtatagct cacgttcagg
     4021 tactcgccga gaccggcggc gtcgacagcc gagatgaccg agtcagggtg ctcgccttca
     4081 atggcgatcg tccggactgt gcggtcgcca acgtcgaggt cggtcacctc cgcaatgcaa
     4141 tcgtccaact ggtagcggac cctcgtcttg tgaacctgca ccgcctggag aaccctgtcg
     4201 gctgcgacga cctcttcgag gaattggctc atcgtgtact gatcgcgtgc aaggtcgggt
     4261 gccgggcgtt ccagcacggc gaagacctcg aggacgtcgt ttgcagggat cggggacccg
     4321 accttcatca tcggagtcca tagctccaac ccgcggctgt cgacttcctt cagaactttg
     4381 acgtcgacca ggtcatcacg gatcttgacg ttcgccgcca cctcacccac caaatagaga
     4441 tcgtcgctgg tcttggcctc gatcgggtca agcgccgaga agtagtcctc cgctgtgggg
     4501 aaggacgcgc cgaacgtccg ccactcccac cggggcacga ttgtggccac ttgctcctct
     4561 ccttacgcgg tctcggcggc gttgaccccg tccgggtcgg ccgcaggagg tgagtgtcct
     4621 accggcaccg ttgggatccg gttggtgaag aagagcccac tcagggcgag caccgcgagc
     4681 acggagagac tggcgcgcag ccctgcgata cgggctgcct cgttgtcgtc aatgatcgcg
     4741 ttcacttgat ccggggggag cccggtctcc gccaaggcag tctccagctg tgcgtcggaa
     4801 ataaacggga tgccgctggc gagctcgact tcggcctggt tcgacacctc gggagggata
     4861 tccgggttgg ccacgactcc ctcgaggaaa gcggctgtga gaccggcaat cagtatcgac
     4921 ccggcgaggg cggtaccgag cgaggcaccc aggttggtca tcgtgttctg caaaccaccg
     4981 acctcccctg tctcctcatc gcggacaccg gaaacggtca ccgctcccaa ttgagaggcg
     5041 agcgcgccca ccccgacccc ggcgagaagc atcgggatcg tcacgatctc ggcaccggcg
     5101 ccgatctcga gcgcgccaac catggagacg atcccgaaga acaggaagat gagcccccat
     5161 cgcaccacgg ttcgtggatt cgcctgcggc ctgtacttgg gaattccaac ggcggcgatg
     5221 aggagggtga cggaaagggg gagaaggcga atcccggtgt ccaaggcgct cagtcccagg
     5281 gccaccgata ggaacagcgg cacgatgaag aacaaaccgg cttggatcaa gaactggaag
     5341 aagaacatga ttagcccgcc ggcgagctgg cgattctgaa gcatctgcgg gcgcaccaat
     5401 ggctcacgtc ctctgcctat caggcgttcc tcccaagcga agaagagcca gacgatgaat
     5461 gagccggcga gtatgagcca tacggtcatg gagagcccaa gccactgagg tgcgtctggt
     5521 ttcggctgca cccatcccca cgtcccggag cggaggacgc cgaagaccgc gaggccaagc
     5581 ccgatcgctg acagcgccac gcctaccagg tccaatggtg gctggggctc gagcggcaca
     5641 tcatgcatct tccggctcaa cgccaggatg gcgatcacca gtaccacttc gcctgcgaac
     5701 acgtagcgcc acgtccagta cgtggttacg gcgccgccga tcagggggcc tgcggccacc
     5761 gcgatggcac cggccgaggc gaccagcccg tatgcccgtg ccctacccga gggcgggaag
     5821 ttggatgcga ccagagccac gatggccggc atgatcagcg cggctccgag gccttcgagg
     5881 aacgaccagc cgatgatcag tacggtcaag gtcggagcca gcgcggtggt caatgagccc
     5941 gctccgtaga tgatgcagcc gatggcgaac gccttgcgac ggccgatcat gctgccgatc
     6001 ttgcctccgg tgatcatcat cgtggccatc accagggtgt agagggtgat cgctgtctgg
     6061 acgccggtga ccgtggttcc gacatcctcg gccacctggg caatggacac gttcatcacc
     6121 gcgctgtcga gggtcatcag gaactggccc gtggaaagtg tcaacaacac cagcccggcc
     6181 ccagccatcg ctttctcggc tgtgctcggg cttgaagagt cggaggagct caagtgggct
     6241 cggcccacgg ttcacgggaa tggatcttcg cccagaggta ggacagaagg acacggacgt
     6301 tggccaccac gggcaccgcg aggatcgcac ccaggatgcc ggcaacttga tatccggcgg
     6361 tgactgagat caggacgacg agagcgggca actggaccga atgccccatg accttggggg
     6421 cgacgatgtt gctctccaac tgctggatga gtgtgtaggc aattatgacc agcagtgcga
     6481 acacgatgtt gttcatgtca acgaagcggg tcgatccttg aaccagcgca atgatcaccg
     6541 ccggaatggt ggcgatgatc gggccgaagg tggggatgac ctcgagcaga ccggcgatca
     6601 cagccaggaa cagggcaccg ggaagcccta tcagccagac gaccacgaac gtggtgaatc
     6661 cgatgacgag gatcatgatc ccttggcccc gtatgtagtc attccatgac gcgttgagcc
     6721 ttcgacccag ttcgaacact tccagctcgt attccttggg caccaggttg gcgatgctcc
     6781 ggaagtaccg actggagtcg gccgtcatat agatggcaat gatcaataca aaaacgatcc
     6841 ctgaaatgat cccaatcagc agcccggcgg cgccggctgc tgccaccaac gcctgggtga
     6901 gggccccgag ccaacgaccc ggatcgagca aatcggcgct ggtggacgcc tgactcgagg
     6961 cgtcgctaac ggccgagaca gcagtgaggt caacgccaag gaagttggtt gaggaaaggc
     7021 tggtcgcgac gttggtgagc caagtctcga aggaggcgac gatctcatgg aaatcgattt
     7081 gagcaagtga gccgatgatc agggccggga gtatgaagac ggcgccgatg gcgatggctg
     7141 cggttgcgag atagaccacc gtcacggcaa gccatcttgg gaagtgaagc ttgttaacca
     7201 gcccgcggag gatgggctcg atcaggaacg ccaggagggc ggcgacgatg atcgagtcga
     7261 ggacgccgaa caacaggaag aagccgaggg cgaccacccc aatcacgagg gcaatggcca
     7321 cgagacggcg ggtcgatgct gaccaggcct gtccgctgat ggggaccagc tccgcttctt
     7381 cgggcgcgat ctgaccttgt tgagccggtg gctgattccc tgcgtcggtc acagccgtga
     7441 aacgtagctg ttgaaggcca acagcgacga gcaatcaccc aggtggtgtg agcaccgtgt
     7501 tcacgacgtg ggctgtgcca ttgctcccaa tcacgttgct cacagtcaca gtcgacacac
     7561 cgttgaggac gagctggtcg ttgtccactg ttattgcaat gtcagagccc tgtgcggtgg
     7621 taacggatga catatccatc aggtcgaagg cgcccaggtt ttcattgacc acgtgataga
     7681 gcaggatcgc agccagggct tcagggtcag agggcagagg ctcggccaac tcggcgaaag
     7741 cgtcatcggt cggtgcaaac agcgtgaacg ggcctggccc ggacagggtc tcggtgagtc
     7801 ccgccgcatc gatcgctgct agcagttgac tgaactcgcc agcgtctgcg aggacctcga
     7861 ggatcccccc tgatggctgg ggcgttgtcg tgtcgggagg cgcaaccgtc gtcgtggcgg
     7921 cggccgccgt ggtcgtggtc tcggcgggca aggtggtggc gggcccgcgg tcgggctcgg
     7981 tcgtcgcagt ggtgggcggg gcctcacctg catcgttgag cgccttctgg aaagcccgca
     8041 acgtggcggc atcgaccaca ccggtggctg gcacgccgag atcggtctga agcgccttga
     8101 gggcgtcctc caactcccga gaccagacac cgtcaacggg gccgtcgtag taaccggtgc
     8161 tgatcaggat tccctgaagg gcaccgatct ctgccgactg acgctgaccc agggcggcag
     8221 caagtgcggc ctgagttgcc gggtcgacca acccggtcac cgggaggcca gcgtctttct
     8281 gcagttgttc tacggcagcg atcgtctgcg gcccgtagat cccatcgatg gcgccgtcat
     8341 aaaggccggc ggcctggagg tcggtttgga gagcggtgac gtagtcggag acccactgtg
     8401 aggcctgggc ctcgtcctcg atgcagccgg catcggcgaa gaggcgaacc catgccacct
     8461 cgaggccata ggcggctgag gtgaagttga cacccgcctc gaccaccgtg gtgttgtcgt
     8521 caatgtcgct ggcggcggcg aaagcagcct cggcgtcctt gaccgcgtcg atcgactcgg
     8581 gctggatgat ctcgaccgtg agaccgggcg tggtgtcggc cgtgacggcc tcctggaacg
     8641 tggccgccga ctcctcgacc gcctcccggc cgggtgccag ctcatctgcg gcattcttga
     8701 catcaccgac ggtgaggtcg acatcctcga acaggccgcc gtagctaccg agtgcggaaa
     8761 cgtcttgctc gacgtccgag cagaactgcg cctgagcggc ggcaacgtcg atcggttctt
     8821 cttcggtgca tcccgagatc gtgagtccga ggacgaccgt cagggcaatg attgcgaatc
     8881 tcgatctcat agcagttccg tcacctcaac tcgaaggatg gccgtggata tgtgatgcca
     8941 agctaattcc aggtcgtcgc tatctgtgtc atccgggctt cctgttggca cgagcctctc
     9001 caccagtgtg tatctttcgg gcgtcgcacc acctagagga gacaagagtg cccttaatgg
     9061 acttgttctg gacaatgctc tggttgttcc tgtttttcgc ctggatctgg ttgttgatca
     9121 cagtgttctc cgacatcttc cgcagtgatg atctgggggg ttgggggaag gcgttgtgga
     9181 tactgttcac agtcttcctg ccctatctcg gagtcctcgt atacctcatc gctcgcggcg
     9241 gctccatgca gcagagaacc cttgccgaac acgctgctcg tgaagagatg accagggcct
     9301 atgtccagca agtcgcgagg agcggctcca cggccgaaga gctcacccag cttgccaacc
     9361 tgcgggacac cggggtcatc accgccgagg agttcaacca gcaaaaggcc aagcttct
//
LOCUS       DBNHXU010000053         7750 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1207929, whole genome shotgun sequence.
ACCESSION   DBNHXU010000053 DBNHXU010000000
VERSION     DBNHXU010000053.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7750)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7750
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1207929"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            201..854
                     /locus_tag="AC161X_01395"
     CDS             201..854
                     /locus_tag="AC161X_01395"
                     /inference="COORDINATES: protein motif:HMM:NF012464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR4988234.1"
                     /translation="MDKRTNSKRKAVLYSRVSTEDQAGSGLGLADQREKLEAEATRHR
                     WEFTHFTDTGSGRTLHHRPDITTALDLLDGGEYDVLAVAKLDRVSRSVADFATILERA
                     QRNGWAFVALDLGVDTTTPTGELIAGILMTVAQWERRVISERTTAALHQAKARGVQLG
                     RKPNLPPEVVIRIITERRSGATLQAICDGLAGDGIPTSQGGIWRPATVRKLVMAYGS"
     gene            851..1315
                     /locus_tag="AC161X_01400"
     CDS             851..1315
                     /locus_tag="AC161X_01400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988235.1"
                     /translation="MNDPLAFISSVTWTFASTMPWAPHEYIVRHRVDRQGFDELALHI
                     RLHGRLGRWGSKLHWYWTLDDWKYWSMGWPLHETIIINRARVSADDTRYLIDGNLIAA
                     DREILDTWCDFTECPACLSREGITAEWEALDLLGYESRLVGLVCPACGHRML"
     gene            1814..2125
                     /locus_tag="AC161X_01405"
     CDS             1814..2125
                     /locus_tag="AC161X_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988236.1"
                     /translation="MPKPFTPPPIDEDLLTIEQLASLLDTTPNSIYVQRNRGLPPGSL
                     GVVFRNRLWWRARDVQRWLDESLAASFDEAWEKRNQRKPEPIVTEARRLADARRAGDV
                     E"
     gene            2215..2466
                     /locus_tag="AC161X_01410"
     CDS             2215..2466
                     /locus_tag="AC161X_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988237.1"
                     /translation="MSSNDFSRMTTPELTRRRRSLEREFQRVRKVFPPDIARLEEIVA
                     ETKAVATEQIKRANQQQPTGRIRGFRPPWAGKKKDDLSQ"
     gene            2522..3865
                     /locus_tag="AC161X_01415"
     CDS             2522..3865
                     /locus_tag="AC161X_01415"
                     /inference="COORDINATES: protein motif:HMM:NF024547.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3987 domain-containing protein"
                     /protein_id="HIR4988238.1"
                     /translation="MTQFSDDHRHVRSPIRWLRDQWDLAATTRFEDMRAQEPSEDEVL
                     SLPWRCRIGLVDVVLNRIETQLEADPLAIASILLTYFGNAMGSKPHVQIGPQAHGGNV
                     YTVLVGDSSSGRKGEAARAVSRIFEHADPYWAANCIQGGFASGEAVIAALAESADPRR
                     LVLEHEQAATLARAGREGSILSAVLRNGFDGGPLENRRAGGMVVAKGHHVSSVGMITP
                     EELGRSLTTVEASNGYGSRHLWLRVQRQRRLPFGGDIPDVAWASLGDEVGACLEEARR
                     VDVLEWTDDGRSLWKDLYARTPDVFGLARALLSRRETIMLRLAIIYALSDGRRRIDVD
                     HVIAASALWERSVSSVKTIWQQSTGDPLADAVLVELRHRSLTQTEVRQVLGHNYSAAR
                     IQDIVDVLLGTGLVDLTERKTAGRPAQVLSYREPSPEDFPPISPFSPNEEEKRHR"
     gene            4122..4301
                     /locus_tag="AC161X_01420"
     CDS             4122..4301
                     /locus_tag="AC161X_01420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988239.1"
                     /translation="MSELFQCDYCGRLRPLNDAVLAWAEDADRETKDGDSVFTVEVTC
                     GPSCAEKVNAIRVRR"
     gene            4551..5000
                     /locus_tag="AC161X_01425"
     CDS             4551..5000
                     /locus_tag="AC161X_01425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988240.1"
                     /translation="MSAISKPVGVILVGMLCVVFGAIIAIIALGLGLGNFFVFLDPQP
                     TWLLVLSCAFGLVVAYLATRVGLGLLRDPPTGRKSSGIAMWVGIPIVWLTSVGLNLLI
                     SQGQDDVGAWFPAGMFGVLWTAVLVAAGLYMRSRGVRAYFDEATPKL"
     gene            complement(5150..5662)
                     /locus_tag="AC161X_01430"
     CDS             complement(5150..5662)
                     /locus_tag="AC161X_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988241.1"
                     /translation="MADQAYPHLIRIRRWLYLGLGLLFGGFALLAWFEQTRGLLLDDG
                     SGSLTAPWVPIVFLASLVITPLVLFDFVRWLLKGGFGVRTRVISLLGILLAFFGGYFL
                     TWNASICFKEGGVPNHPEPGAGYDEVFGCYGPGDPTLSIAVGLICACIGAYLVWLVLR
                     NRGAMVSQPV"
     gene            5691..7157
                     /locus_tag="AC161X_01435"
     CDS             5691..7157
                     /locus_tag="AC161X_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013556463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HIR4988242.1"
                     /translation="MSSTVALVGGEARPVEVQAHVGRQIEAFKLSGLPDTALREAKDR
                     VRAAVLSAGIRFPNRTVTVNLAPADLPKSGTDYDLPIALGILAASREIPSLQRAVVVG
                     ELALDGAVRAGSNALGAGVLSASQGVPCLVASSVAAEAAAIPGARVYGVSTLADAVDM
                     LRDGLEGRAPATPDEPRRHDEALPDMSEIRGQMVARRALEVAAAGGHHLLFHGPPGSG
                     KTMLARRMIGILPRLEIAEAIETAMLWAAAGRRRGLDTTPPFRSPHHTASKAALVGGG
                     SGNPVPGEASLAHHGVLFLDELAEFPRGHLDTLRQPLEEAVVSVARRGMSVDFPASFQ
                     LLAASNPCPCGFHGDHRKPCECRPAIRWRYRSRISGPLLDRFDLIVHVGRVEANDYSG
                     DPGEASIDVADRVQRAREIQSSRGQINRRLTPSALPESVGARSLVASAVGSALLTARG
                     ADRVRRVARTIADLAGVEQVEEEQMAEAIGLRGEWRDD"
     gene            7150..>7750
                     /gene="dprA"
                     /locus_tag="AC161X_01440"
     CDS             7150..>7750
                     /gene="dprA"
                     /locus_tag="AC161X_01440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR4988243.1"
                     /translation="MTEHRIVKPGDATYPAGLLDLEEPPSLYVLGQTTTAPAVAVVGT
                     RRCTRYGLSLAEGFGRALAQAGWTTVSGLARGIDASAHRGTRGGRGEAVAVLGSGIDV
                     VYPAENRELYHSILAGTGAVISEYPPGTPPDRWRFPARNRLIAAIASAVVVVEASNKG
                     GALITARLAAELGRPVFAVPGDVDRAASVGCNLLIRDGAI"
BASE COUNT         1429 a         2469 c         2488 g         1364 t
ORIGIN      
        1 cccacagacc aggcagacgt cagcggagag ctggacgagc ggcttgctct cggtggttga
       61 tcgcaccgac atgaggccac cataaccctc gtctgtgacg tcgtaaaccc agccgtttcc
      121 ctctccgaaa agcgggcttg accaggacaa atggcgcaaa gggccgttag gtaacccgac
      181 cacccctggt acaatgggat atggataagc gaacaaatag caagagaaaa gcggtcctct
      241 actcccgggt ctccaccgag gaccaggctg gctccggcct cggtctggct gaccagcgag
      301 agaagctcga ggccgaggcc acccgtcacc gctgggagtt cacccacttc accgacaccg
      361 gctccggtcg cactctccac caccggccag acatcaccac cgccctcgac ctcctcgacg
      421 gtggggagta cgacgtcctc gccgtggcca agctcgatcg ggtctcccga tcggttgccg
      481 acttcgccac catcctcgaa cgagcgcagc gaaacggctg ggccttcgtt gccctcgacc
      541 tcggggtgga caccaccact cccaccgggg agctgatcgc ggggatcctg atgaccgtgg
      601 ctcagtggga gcgccgggtg atctcagagc gaactaccgc ggctctccac caggcgaagg
      661 cgcggggagt ccaactcggc cgcaaaccca acctgcctcc ggaggtggtg atccggatca
      721 tcaccgagcg tcgaagtggc gccaccctcc aggcgatctg tgacggtctc gccggtgacg
      781 ggatccccac cagccagggc ggcatctggc gaccggccac cgtccgcaag ctggtcatgg
      841 cttacgggtc gtgaacgatc cgctggcctt catctcatcc gtgacctgga cgtttgcctc
      901 cacgatgccc tgggcgcccc atgagtacat cgtccgccat cgggtcgatc gccagggctt
      961 cgacgagctc gccctccaca tcaggctcca tggccggctc ggtcgatggg ggtcgaagct
     1021 ccactggtac tggaccctcg acgattggaa gtactggtca atggggtggc cccttcacga
     1081 gacgatcatc atcaaccgcg ccagggtctc cgctgacgac acccgctacc tcatcgacgg
     1141 gaacctgatc gccgcagatc gggaaatcct cgacacctgg tgcgacttca ccgagtgccc
     1201 tgcctgtctg agccgggagg ggatcaccgc tgagtgggaa gcactcgacc tcttggggta
     1261 cgagtcccgg ctggtgggcc tggtctgccc ggcctgtggg caccggatgc tctgagaggc
     1321 cgcaggatcg ctcagacggg ggaaagcgtg ccaaacgtgg cacagatacc gaaccggccc
     1381 aggtttgggc cagtaagggc aattgcgggg cgctcagttg tgccaggggt ggtcggaccc
     1441 gatcagatcg agggtgagga cgaggcccct tgaggatggt cgagggtggg tcccgaccga
     1501 ggccccgccg ccaccctgac cccggggggc atgagttggt tccccaaggt ctcccggtca
     1561 gcttacgggt agatccccag agttatatcg gctccgattt gctagcaacc agggagctat
     1621 ctctgcccca catggatcgg ctccacggcg gttgccagcc gtggtgaggt cgagtaagat
     1681 ggactgataa gggagacagt ctccctgctg cgcacaggat cacgtctcag tcaccgaggg
     1741 cgcctgtctc cgcgttgggt aaacgtcacg ctggcggttg cggcaccagc acctaccgaa
     1801 aggtggtgca cgtgtgccca agccattcac tcctcccccg atcgatgagg atctgctgac
     1861 tatcgagcag ctggcgtccc tcctcgacac cacgcccaac tcgatctacg tccagagaaa
     1921 ccgcggccta cctccaggat cgctcggggt ggtcttccgc aatcggctgt ggtggagggc
     1981 ccgggacgtc cagcgctggc tcgacgaatc ccttgccgcg tccttcgacg aggcctggga
     2041 gaagcgcaac cagcggaagc ccgagccgat cgtgaccgag gcccgtcgcc ttgccgatgc
     2101 gaggagggcc ggcgatgtcg agtgacggcc catcagacga ggccctgcgt cgagcagcgg
     2161 tcgttctgag ggagatcgcc cttcgactcg ccaccgagca agaggaggaa cccgatgtcg
     2221 agtaacgact tcagccgcat gaccaccccc gagctcaccc gacgtcgaag gtcgttggag
     2281 agggagttcc agcgagtgcg gaaggtcttt cctcccgata tcgcccgcct cgaggagatc
     2341 gtggccgaga ccaaggccgt cgccaccgag cagatcaagc gggccaacca gcaacagccc
     2401 acgggccgga tccgcgggtt ccgcccaccc tgggccggaa aaaagaaaga cgacctctca
     2461 caatgagagg ccgtccttcg cgactgggtg tcgaccgagc caactcatgg gaggtgactc
     2521 attgactcaa tttagcgatg accatcgaca tgtccggtct cccattcgct ggcttcgcga
     2581 ccagtgggac ctggcggcga cgacacgctt cgaggacatg cgggcccaag agccatccga
     2641 ggacgaggtc ctcagcctgc cttggcggtg tcggatcggt ctcgtcgatg tggtcctgaa
     2701 tcgcatcgag acccaactgg aggcggatcc cttggcgatc gccagcatcc tcctcaccta
     2761 cttcggcaat gcgatgggat cgaagcccca tgtccagatc ggcccccagg cacacggggg
     2821 aaacgtatat acggtcctgg tcggagattc gagcagcggt cgaaaaggtg aggcggcccg
     2881 ggcggtgtcg aggatcttcg agcacgccga tccgtattgg gctgccaatt gcatccaggg
     2941 cggattcgcc tcgggggaag cggtcatcgc cgccctggct gaatcagcag accctcgacg
     3001 tctggtgctt gagcatgagc aggcagccac cctggcgcgc gccggacgcg agggatcgat
     3061 cctctcggcg gtgctccgca atgggttcga cggcggccct ctcgaaaacc gacgggcagg
     3121 cgggatggtg gtggcgaagg gtcatcacgt ctcctcggtg gggatgatca caccggagga
     3181 gctgggccgc tcactgacca cggtcgaggc atccaatggc tatggctctc gccatctctg
     3241 gctgagggtc caacgtcagc gccggcttcc ctttggtggg gatatccccg acgtcgcctg
     3301 ggcatcactg ggtgacgagg tgggtgcctg tctcgaagaa gcccgtcggg tcgatgtcct
     3361 ggagtggact gacgatggtc ggagcctctg gaaggacctc tatgccagga ccccggacgt
     3421 gttcgggttg gcgcgagctc ttctctctcg acgggagacg atcatgcttc gcctggcgat
     3481 catctacgcc ctctctgatg ggcggcgtcg gattgatgtc gatcacgtga tcgcagccag
     3541 cgccctctgg gagcgctcgg tgtcatcggt gaagacgatc tggcagcaat cgaccggcga
     3601 cccgttggcg gatgcggtcc tggtcgagct tcgacaccgg tccttgaccc agaccgaagt
     3661 ccgccaggtg ctgggtcaca actattcggc cgctcgtatt caggacatcg tcgatgtgtt
     3721 gctcgggacc gggctggtcg atctcaccga gaggaagacc gctggccgac ccgcccaggt
     3781 cctctcctac cgggagccgt cccctgagga ctttcctccc ataagtccct tttctcccaa
     3841 tgaggaagag aagaggcatc gatgagggaa gtcccagcat cccccctgga gggctttcct
     3901 cccaaaactc ccttaagtcc cgttaaggaa atgagagatg gagaggagaa agagcctccc
     3961 tcggagtctc tcgcggggtc tctctcagaa cctctcccag agggagatat gggagatagg
     4021 ggagaaaagg cttcctctct cgaggaccgt gaccgggccc gggagcaatc cccggctgaa
     4081 ttcaccccca tcgacgttct tcactccgaa ggagcatcga catgagcgag ctcttccaat
     4141 gcgactactg cggacggctc cgtcctctca acgacgccgt gctcgcctgg gcggaggacg
     4201 ccgatcgcga gaccaaggac ggtgactcgg tcttcaccgt cgaagtcacc tgcggtccgt
     4261 cctgcgccga gaaggtcaac gcgatccgcg tgcggcggtg agcgacaact gagcgaccct
     4321 tttggtatac gaaggccgga tacctaacgc tcagttggga taaaagccca ggtctaagtc
     4381 ccttttcttg tgcttcagga tgtcagcggt ttcccttggc gcggttgtgg gtgacgcaga
     4441 gcatctcgca gttgtcaagc acagtggagc gtgtggggtc aaactcgccg catttgcgct
     4501 cgcggggaaa cccgtttccg cgcatacggc ggtcgtagcc tgaatcgaca gtgagcgcga
     4561 tctcgaagcc cgtgggcgtc atcttggtcg gaatgctctg cgtggtgttc ggagcaatca
     4621 tcgcgattat cgcccttggg ctcggccttg ggaacttctt cgtcttctta gacccccagc
     4681 cgacctggtt gctggtgctg agctgcgcct tcggcctggt agtcgcttac ctcgcgacca
     4741 gggtcggcct cggcctcctg cgtgatccac caacaggcag gaagtccagc gggatcgcca
     4801 tgtgggttgg cattccgatt gtgtggctga caagtgtcgg cctcaacctt ctgatctccc
     4861 aaggtcagga cgatgtgggc gcatggttcc cggcgggcat gttcggagtg ttatggactg
     4921 cagtcctggt tgccgcaggc ctctacatgc gatcgcgagg cgtgcgcgcc tacttcgacg
     4981 aggccactcc aaagctctaa tcgagcgcag cgaagggccg agagaggatg gccgtcactc
     5041 acgctcaaac tttgtcaagc acagttgagc gggttgcggg gaatgatcag cagcgacggg
     5101 ttagaagcca gcgtccggcg ggccggcgtt tcgttgctat cgatggatgt cacaccggct
     5161 gcgagaccat cgcgccgcga ttcctcagta ccagccaaac caggtaggca ccgatgcagg
     5221 cgcagatcaa accgacagcg atcgagaggg tgggatcgcc gggtccgtaa cacccgaata
     5281 cctcgtcata ccccgctccg ggctctgggt gattgggcac gcccccttcc ttaaagcaaa
     5341 tcgaggcatt ccaggtcagg aagtaaccac cgaagaacgc cagcaggatg ccaagaagcg
     5401 agatgacgcg ggtccggacc ccaaatccac ctttcagcaa ccagcgcacg aagtcgaaaa
     5461 ggactagtgg tgtgatcact agcgaggcga ggaaaacgat ggggacccaa ggggcagtca
     5521 gcgaaccgga cccgtcatcc aagagcaaac cccttgtctg ctcgaaccaa gccagcaacg
     5581 cgaaaccgcc gaacagcaag cccagcccga gatagagcca ccttcggatt ctgatcagat
     5641 gagggtacgc ctggtctgcc atccatttgc gcaaggggtg ccgagaaaac ttgtcaagca
     5701 cggtggcgct cgttggggga gaggcccgac cggtggaggt ccaggcacac gtcgggcgtc
     5761 agatcgaggc tttcaagctc tccgggctgc ccgacaccgc ccttcgggaa gcaaaggacc
     5821 gggtgcgggc cgcggtgctt tccgccggaa ttcgcttccc caatcggacg gtgaccgtga
     5881 acctcgctcc ggccgacctc ccgaaatcgg gaaccgacta cgaccttccg atcgcactcg
     5941 gcatcctcgc cgcaagtcga gagatcccaa gcctgcaacg ggctgtggtg gtgggcgagt
     6001 tggcgctgga cggagcggtc cgggccggat cgaatgccct cggagcaggg gtgctgagcg
     6061 ccagccaggg tgttccctgt ctcgtggcct cgtcggtggc ggctgaagcg gcagccatac
     6121 ctggggccag ggtgtacggc gtctccaccc tggccgacgc cgtcgacatg cttcgtgatg
     6181 gcctcgaggg cagggctccg gcaactccgg atgaaccgag gcggcacgat gaagcccttc
     6241 ctgacatgtc cgaaatcaga ggccagatgg tggcgagacg cgctctggag gtagccgctg
     6301 ctggtgggca ccaccttctg ttccacggcc ctcccgggtc gggcaagacc atgctcgccc
     6361 gccggatgat cgggatcctt ccccggcttg agatcgccga ggccatcgaa actgcgatgc
     6421 tctgggcggc cgcagggcga cgccgcgggc ttgacaccac acccccgttt cgctcgccgc
     6481 atcacaccgc gtccaaggcg gctctggtag gcgggggcag cgggaacccc gtgcccggtg
     6541 aggcgtccct cgcccatcac ggcgtgctgt tcctggacga gctcgccgag ttcccacgag
     6601 gtcatctcga caccctccgt caaccactcg aggaagcggt agtttctgtc gctcgtcgcg
     6661 gcatgtctgt cgattttccg gcttcgtttc aactcctggc cgcgagcaac ccctgccctt
     6721 gtggctttca cggcgatcac cgcaagccat gcgagtgccg gccagcgatt cggtggagat
     6781 acaggtcgcg gatctcgggc ccactcctcg accgattcga cctgatcgtt catgtcggcc
     6841 gggtcgaagc caacgactac tcgggggacc ctggcgaggc cagcatcgat gtcgccgacc
     6901 gtgtgcaaag ggcacgcgaa atccagagct ccagagggca aatcaaccgc cgcctcacac
     6961 catccgcgct tcccgagtct gttggagccc gctccttggt ggcatcggcc gtggggagcg
     7021 ccctgctcac cgctcgtggg gccgaccggg tgcgccgggt ggctcggacc atcgccgacc
     7081 tggctggggt cgaacaggtc gaggaggagc aaatggccga ggcgatcggc ctccggggcg
     7141 aatggcgcga tgactgagca ccggattgtc aagccaggcg acgccaccta cccggcaggg
     7201 ctcctcgacc tcgaagagcc accctccctg tacgtacttg ggcagaccac taccgctcct
     7261 gcggttgccg tggtcggtac caggcgatgc acccgatacg gcctgagcct tgccgagggg
     7321 ttcggacgcg ccctcgccca ggcaggctgg accactgtca gcggccttgc tcgcgggata
     7381 gatgcatccg cccaccgcgg cacccgtgga ggccgtggcg aagcggtcgc cgtgctggga
     7441 tccggaatcg atgttgtcta tccggccgag aaccgggagc tgtatcactc gatactggct
     7501 ggaacagggg cggtcatctc cgagtaccca cctggcacac caccagatcg atggcggttt
     7561 ccggcgagaa accggctgat tgccgccatc gcctcggcgg tggtggtggt cgaggcaagc
     7621 aacaaggggg gtgcgttgat cacggcgcgt ctggctgccg agttgggacg ccccgtgttt
     7681 gcggttcccg gggacgtcga ccgggcggca tcggttgggt gcaacctcct gattcgagac
     7741 ggggcgattc
//
LOCUS       DBNHXU010000054        11025 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2794775, whole genome shotgun sequence.
ACCESSION   DBNHXU010000054 DBNHXU010000000
VERSION     DBNHXU010000054.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 11025)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11025)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11025
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2794775"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1305
                     /locus_tag="AC161X_01445"
     CDS             22..1305
                     /locus_tag="AC161X_01445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR4988244.1"
                     /translation="MGKHAELYERHQKVLPGWAPLYYQQPIAIVDGDGRHVVDAEGNR
                     YLDFFGGILVTMTGYRVPEVVDAIKRQADRMLHTSTLYLIESQIELAERVAELSGIPD
                     AKVFFTNSGTEANDAALMLATTYRKSNQVLAIRGSYHGKSHSTIAITGQKAWSGTSFS
                     PFQVTYVHGGYKLRSPFGHLSDEDFIAACVADLEDLLRVASAGEPAAMIAEPIQGVGG
                     FVTPPDGFFGAMKEVLDAHGILFISDEVHTGWGRTGEHFWGYQAHGITPDLLTFAKGI
                     GNGLSIGGVVAPAEIMEAYPALGISTFGGNPLATAGALANLEVLLDQDMQTNALKVGN
                     RLMQNLIPLSEELEMIGEVRGKGLMIGLELVGPDGEPAPEKAAAALEAAREEGLLIGK
                     GGIYGNSLRIAPALSLTEEEADDGFERLERALRRI"
     gene            1453..1665
                     /locus_tag="AC161X_01450"
     CDS             1453..1665
                     /locus_tag="AC161X_01450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988245.1"
                     /translation="MNLAVGILCPRCGHDVANLIEFVKDDSQVLVANRLQCVRCETVY
                     LEARELTDHEEAFPLEWVVDEAFGRT"
     gene            complement(1729..2679)
                     /locus_tag="AC161X_01455"
     CDS             complement(1729..2679)
                     /locus_tag="AC161X_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015439938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR4988246.1"
                     /translation="MGKPIRASDARPERGSGPGDTVGLYLDEVSAHLLLTAEDEVHLA
                     RSMERGKKAKSLLETGDFQVAERPQLMRLIAEGEEAKMTFIRSNLRLVISIAKRYTGR
                     GLDLLDLIQEGNLGLIRAVEKFDWRKGFKFSTYATWWIRQAITRGLGNNGRTIRLPVH
                     MVDVVRTVQDTKQSLHDRYHRAPTLEEISEASGLDTERVLAALEAPAETISLDRPVGE
                     DGDASLQDFVEDTGAANPEDRAASAWRRERLIKALQILDLEEQQVLWLRFGLDGDEPW
                     TLSDVGKVINSTRERVRQIEARGLAKLRHPSCDIDLQALL"
     gene            2784..4247
                     /locus_tag="AC161X_01460"
     CDS             2784..4247
                     /locus_tag="AC161X_01460"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024598.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycoside hydrolase"
                     /protein_id="HIR4988247.1"
                     /translation="MSRRSQFRLLVAFTIVWAVWAGWSLLGRATPYTLRVLDDSGAPV
                     ASAFVEVNGSQVGTSADDGLVDMDWNSSNSVLEVSAPGHVPLTITLTDRPEGVVDAVL
                     KARVLRGKVVDGSGLPVEAVQVVAGPASGVSDEEGEFTIRGAEPGAVQVTRPAWVDTS
                     FDWDGGPGEASVELLPFAARAVHISGEATADNLDHYIEMAEATELNALMIDLKDETGL
                     VWYNSQDSVADEVGAIRSLYDLTDVSARAHEHGLYLIGRLVIFNDPTAAVKKPSMAVW
                     DTATEAPFESNNQYFLDPTDPDARAYALSLAEEACSMGVDEIQFDYVRFPDQRSDTTV
                     FDGGITQEVRIEAITSFFHEAIAVLHPMGCAVGADVFGFTTKAEDDGGIGQRWPDILQ
                     IVDVASPMVYPSHYSTGWYGFDVPNDHPGEMVTNALEDGLARMPRNVVIRPWLQDFGY
                     TPDQVRAQIDSAEQYGLGWMLWNAKSNVTMDALGPAE"
     gene            4303..5565
                     /gene="sufD"
                     /locus_tag="AC161X_01465"
     CDS             4303..5565
                     /gene="sufD"
                     /locus_tag="AC161X_01465"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HIR4988248.1"
                     /translation="MPSFDSENLEKLIAAAPPWRRDGMLAAHGIFESLEEPTGAEEDW
                     RYVQFETPFSTLVSIDEPGASMGGGPFLASVDRTAGRVLIVDGAVLEAVSDGDLVKRF
                     ADLSQEPTRDALVPVDHNKLAAAHAAFSTDGALIEIPKGAVIADPLVIEIQGVTPNAA
                     SFPHVIVQVGENAEASVLVVYRSADDARLLMVPEIDLQVAAGGRLRYLAVQGLDQAAT
                     SVVHQRVTLSRDATARIGEVGLGAKLGRLDLGIDLIGDGSSSEVVGLYFGEGEQTLDY
                     RMVVTHHGKSTTSDVFLKGAVEDDAQSVFTGLLRIEKDARGTSTFETNRNLVLSENAK
                     AQSVPNLEILCDDVVCGHGSSVGPLEQEHLYYLQSRGLTRERAERLLIRGFFQEVIDR
                     LPIEAVGGPVSDEVFRRFVSAQEEGRVA"
     gene            5562..5873
                     /locus_tag="AC161X_01470"
     CDS             5562..5873
                     /locus_tag="AC161X_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018811709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rieske (2Fe-2S) protein"
                     /protein_id="HIR4988249.1"
                     /translation="MKIELGALEALPRDRGIRVTVGDTRVAVFRVADEVFAIGDRCSH
                     AEASLAEGEVYGTEVECPRHGSEFELRTGEPISLPATQPVPTYEVKIEDGNVYLFLEL
                     P"
     gene            5870..6670
                     /gene="sufC"
                     /locus_tag="AC161X_01475"
     CDS             5870..6670
                     /gene="sufC"
                     /locus_tag="AC161X_01475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020613851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR4988250.1"
                     /translation="MTAALKIKGLSASVASKQILQDLDLDVPYGEIHAIMGPNGSGKS
                     TLCHVLTGKAGYEVSGSATLDDVDLFSLTVDERSRLGLLQSFQYPTEVPGVRLREFLL
                     EATEERGVDPEVAATRIEAEAERFDTTRFLDRSLNNDLSGGEKKRSEIFQMAVLRPKL
                     ALLDEIDSGLDIDAVRQVSEAVESMRSPDLGVVMITHYSRILRYLMPDRIHVMIDGRI
                     VASGGAELAQELDTGGYESVRDRLGLQRASGEPVRKASDFFTDTPFDV"
     gene            6676..7773
                     /locus_tag="AC161X_01480"
     CDS             6676..7773
                     /locus_tag="AC161X_01480"
                     /inference="COORDINATES: protein motif:HMM:NF017181.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988251.1"
                     /translation="MADRSTAERWLAALTNSPTASGHEDAVIRWVRAWVARREDLSLR
                     SDAGGNLLITQKGRRTRSPVVAVAHMDHPAFVVTSVEGREARYEFRGGVRPEYFQNAR
                     VVFPSRGPAIKGVVQTHDPKTGTGSIRVTGNGAVNPGAIATWGFSGQRTKAGHFSAPA
                     CDDLAGCAAGLAALDRARATPALRHFGVLLTRAEEVGFVGAIHAAKTGTVSRETRLLS
                     IEASRASAHAPVGAGPVVRVGDASTVFDRELTNQVSWAAARRGLPHQRKLMAGGSCEA
                     TAFGVYGYRAAGLCLPLENYHNMGNLDEVESGSGKATPMLETIALSDFHALVDLLLLA
                     AEAVDDVGGLETRLDHLYEGSRHLLERRPLP"
     gene            7694..9190
                     /locus_tag="AC161X_01485"
     CDS             7694..9190
                     /locus_tag="AC161X_01485"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018845701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045239 - tricarboxylic acid cycle
                     heteromeric enzyme complex [Evidence IEA];
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0006106 - fumarate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HIR4988252.1"
                     /translation="MWVAWRRGSTISTKAVGTCSNADRYRKGDPRKGATTGVEQRIER
                     DSMGEVEVPADAYYGASTQRAVDNFPISGLRFGRRFIWALGLLKGSAASVAAQLGHVD
                     AERGAAIRKAADEVMEGSLDGHFVLDIFQTGSGTSTNTNANEVIAARASELLATGADP
                     VHPNDQVNFGQSSNDVIPAAIHIAAVAAIREQLIPALDRLSSSLDIKAREFADVVKSG
                     RTHLMDATPVTLGQEFGGWATQVAKGSERLAKVLPELEELALGGTAVGTGINAPVGFA
                     PAVIALMADRTGYPFREADNHFEAQGSKDAAVMASGAVKTVAVSLSKIANDIRFLGSG
                     PSSGLAEIRIPELQPGSSIMPGKVNPVMSEMVMQVAAQVIGNDATITWGGASGNFELN
                     LMMPVIAHNLLESIEILANAAEAFRAKCVEGIEADRERALALLERNAIIVTALNPHIG
                     YDNGAKIAKESLASGRSIRDLVLESGLMTADELDKALDLKKLTEGGIA"
     gene            complement(9294..9743)
                     /gene="sufU"
                     /locus_tag="AC161X_01490"
     CDS             complement(9294..9743)
                     /gene="sufU"
                     /locus_tag="AC161X_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly sulfur transfer protein
                     SufU"
                     /protein_id="HIR4988253.1"
                     /translation="MSLEDLYREVILDHYRTPRNKGHLDAPDAKAQGINPLCGDDITI
                     ELMYDGDTVGSVAIDGQGCSISLSSASMMSEAIKGKSRAEIEELTAKFRGLMSLDDGI
                     DPGVDHDHPGAALGDLEALEGVRQYPVRIKCASLAWNVLAEALASPD"
     gene            complement(9740..10954)
                     /locus_tag="AC161X_01495"
     CDS             complement(9740..10954)
                     /locus_tag="AC161X_01495"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006322747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - obsolete cysteine metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HIR4988254.1"
                     /translation="MDLSHLKADFPVLGDGVHFLDSGASSQKPIPVLEAMDRFARTSY
                     ANVHRGAYKLSVASTDAYEAARNRVAEFLGVKDPREIIFTRGATTSLNTVAFGWGLEN
                     LKEGDRILSTEMEHHANLVPWQIVAKRTGATLDHIPVTDDFRLDMEAYQQKMGPDVKI
                     VAVTGMSNVLGTMPPVRQIADLAHAQGARVVVDAAQLIPHAPVKVDDLGADFVAFSSH
                     KMLGPTGIGILWGKMDALQEAEPFEFGGEMISEVTLQHSTWAEIPHRFEAGTPPIIEA
                     VGLHAAIDYLDSIGMDKIFEHDRALTEVALDRLGDVPGLKIQGPPNTIDRGGVLSMTM
                     DEVHAHDLATILDEEGVSVRSGHHCARPLMQKLGVAATARASFYVYNTEEDIDALVLA
                     LRHARDLFGVGK"
BASE COUNT         2045 a         3352 c         3502 g         2126 t
ORIGIN      
        1 tggctcggta acctcgcgct gatgggaaag cacgccgagc tctacgagag acatcaaaag
       61 gtcttgcccg gttgggcacc gctttactac cagcagccga tagcgatagt cgatggcgac
      121 gggcggcacg tggtggacgc cgagggcaac cgctacctcg acttctttgg ggggatcctg
      181 gtcacaatga ccggctatcg ggtccccgag gtggtcgacg cgatcaaacg ccaggccgat
      241 cggatgctgc acacctcaac cctctatctg atcgaatccc agatcgagct agctgaaaga
      301 gtggccgagc tctcggggat ccccgacgcc aaggtgttct tcaccaactc ggggaccgag
      361 gcaaacgatg ccgccttgat gctcgccacc acctaccgca agtcgaacca ggtgctggcg
      421 atccggggca gctaccacgg gaaatcgcac tcgaccatcg ccatcaccgg gcaaaaggca
      481 tggtcgggga ccagcttctc cccatttcag gtgacctacg tccacggcgg gtacaagctt
      541 cggagcccgt ttggccactt gtccgatgag gacttcatcg ctgcctgtgt cgccgacctc
      601 gaagatctgc tccgggtcgc gagtgcggga gagccggcgg ccatgatcgc tgagccgatc
      661 cagggtgtag gcggctttgt cacccctccg gacgggttct tcggggccat gaaggaagtt
      721 ctcgacgctc acgggatact tttcatatcc gacgaggtgc acactggttg gggacgcact
      781 ggcgagcatt tctggggcta tcaggctcac gggatcacac ccgatctgct cacctttgcc
      841 aaaggcatag gcaacggcct gagtattggc ggggtcgtgg ccccggccga gatcatggag
      901 gcctaccccg cactgggaat ctccaccttc gggggtaatc cgctcgcgac cgcaggtgcc
      961 ctggccaatc tcgaggtact cctcgaccag gacatgcaga ccaacgcgct gaaggtcggg
     1021 aatcggctga tgcagaatct gattcccttg tcggaagagt tggaaatgat cggtgaggtg
     1081 agaggcaagg gtctgatgat cgggctcgaa ctcgtcggtc ccgacggaga gcctgctccc
     1141 gagaaggccg ctgccgcact cgaggccgct cgtgaggagg gcttgctcat cggcaaggga
     1201 ggcatttatg gcaattccct gcggatcgcc cccgcccttt ccctgaccga ggaagaggcg
     1261 gacgacgggt tcgaacgctt ggagagagcg ctcaggcgga tctagccccc ctcgcccgac
     1321 ctcaggtccg gcggttggtg gaggcaatag gggtgttccc ccaggggggt gatccccctc
     1381 ctgttcttgc tcggactaat gcacgatggg gtgcatgagc aaacaagcac agatcacagc
     1441 gtgtggtgtg tcatgaacct cgccgttggc attctctgtc ctcgatgcgg tcacgacgtg
     1501 gcgaacctca ttgagttcgt caaggacgat tcccaggtcc tggttgccaa tcgtcttcag
     1561 tgtgtgcggt gtgagaccgt ctacctggag gccagggagc tgactgacca tgaggaagcc
     1621 ttcccattgg agtgggtggt cgacgaggcc ttcgggcgga cctagccgcc gaatccccct
     1681 catcaagtgg ggaccgacgc agcggctagc tgcgccgatg gggggttact aaagaagagc
     1741 ctgaagatcg atgtcgcagc tcgggtggcg caacttggcc aggcccctcg cctcgatctg
     1801 gcggacacgc tctcgtgtcg agttgatgac ctttcccacg tcagagaggg tccacggctc
     1861 gtcaccgtcg agcccgaaac gaagccacag cacctgctgc tcttcgaggt ccagaatctg
     1921 aagagccttg atcaaccgct cgcgtcgcca ggcggaagcg gctcggtcct cgggatttgc
     1981 ggccccggtg tcctcgacga aatcctggag cgaagcatct ccgtcctcac ccaccggccg
     2041 gtctagcgaa atcgtctctg caggggcctc cagtgccgcg agcacccgct cggtgtccag
     2101 acccgatgct tccgaaatct cctccagcgt gggagcacgg tggtatctgt catggaggct
     2161 ctgcttggtg tcctggaccg tgcgcacaac gtcgaccatg tgcacaggaa gccgaatggt
     2221 tcgcccgttg ttgccgagcc cacgtgttat cgcctgacgg atccaccatg tcgcgtaggt
     2281 cgagaacttg aaccctttgc gccagtcaaa cttttccaca gcacggatca gcccgagatt
     2341 gccctcctgg atgaggtcga ggaggtcgag cccgcgcccg gtgtatcgct ttgcgatcga
     2401 gatgacgagc cgcaaattgg agcggatgaa ggtcatcttg gcctcttcgc cctcggcgat
     2461 gagacgcatc agttgcggtc gttccgcaac ctggaaatct cctgtttcga gcagggattt
     2521 tgccttttta ccccgctcca ttgacctggc gagatggacc tcgtcttcgg ccgtcaggag
     2581 aaggtgtgca gatacctcat ccagatatag gccaacggtg tcgccggggc ctgagccccg
     2641 ctccggtctc gcatcactgg ccctgatggg cttccccaat ctgacctcct caacaacccc
     2701 tgttgaaaac taaccctcct ggctgaatct ggcaaaccaa aatctcggct aacggatttg
     2761 ccctgggtaa cctccatttc tcagtgtctc gccgatccca gttccgtttg ctggtggcgt
     2821 tcacaatcgt ctgggcagtg tgggccggct ggtcactgct cggccgagcc actccctaca
     2881 cgctgcgtgt cctcgacgac tcgggtgcgc cggtggcatc cgcttttgtc gaagtgaacg
     2941 ggagccaggt cggcaccagt gccgacgatg gcttggtcga catggactgg aactcatcca
     3001 actctgttct cgaagtgagc gcgcccggcc acgtcccgtt gaccatcacc ctgaccgatc
     3061 gtcccgaggg cgtggtcgat gcggttctca aggcccgtgt gctcagaggg aaggttgttg
     3121 atggctccgg gctgccggtc gaggcagttc aagtggtcgc ggggccggca agtggggtga
     3181 gcgacgagga aggggagttc accatccgcg gtgcggagcc gggagcggtc caggtcaccc
     3241 gtccggcctg ggtggacacc agcttcgatt gggatggggg ccccggggag gccagcgtcg
     3301 agctgctgcc attcgcagca cgggccgtcc atatctcggg cgaagccact gccgataacc
     3361 ttgaccacta catcgagatg gcagaggcga ccgagctcaa cgctctcatg atcgacctca
     3421 aagatgagac tggccttgtc tggtacaaca gccaggactc ggtggccgac gaggtgggcg
     3481 cgatcagaag tctctacgac ctcactgacg tgtcggctcg cgcccacgag cacggtctct
     3541 acctgatcgg ccggctggtg atcttcaacg atccaacggc tgcagtgaaa aagccttcga
     3601 tggcggtatg ggacacggct accgaggccc catttgagtc aaacaaccag tatttcctcg
     3661 acccgactga tcccgatgcc cgcgcctacg ccctttcgct cgcagaggaa gcatgttcga
     3721 tgggggtgga cgagatccag ttcgactacg tgcgcttccc cgaccaacgc tccgatacga
     3781 cagtgttcga cggcggcatc actcaggagg tgagaatcga ggcgatcact tctttcttcc
     3841 acgaggcgat tgctgtcctt cacccgatgg gttgtgccgt cggagccgat gtgttcggat
     3901 tcaccaccaa ggccgaggac gatggtggaa tcggtcagag gtggcctgac atccttcaga
     3961 tcgtggacgt cgcctcgccc atggtctatc catcgcacta cagcactggc tggtacggct
     4021 tcgatgtccc caacgatcat cctggggaga tggtcaccaa cgccctcgaa gacgggttgg
     4081 caaggatgcc tcgcaacgtc gtgatccgac cctggctcca ggatttcggc tacaccccgg
     4141 atcaggtccg ggcgcagatc gactccgccg agcaatacgg gttggggtgg atgttgtgga
     4201 acgccaagtc gaatgtgacg atggacgctt tgggccctgc cgaataattg gcactatcgc
     4261 cataggtgaa atacgatccg agggtcccac ccggagaggt ctttgccctc gttcgactcc
     4321 gagaatctcg agaagctgat tgctgccgcc ccgccctggc gacgggatgg gatgctcgcc
     4381 gcccacggca tcttcgaatc tctcgaggag ccaaccggcg ccgaagagga ttggcgatat
     4441 gtccagttcg agactccgtt ctcaaccctg gtctcgatag acgagcctgg tgcctcgatg
     4501 ggcggaggtc cattcctggc atcggttgat cgaaccgcgg gccgggtcct gatcgtggac
     4561 ggtgcggttc ttgaggctgt ctccgacggc gacctcgtga aacgattcgc agacctgagc
     4621 caagaaccaa ctcgtgatgc cctcgtgccc gttgatcaca acaagctcgc tgcggctcac
     4681 gctgcgttct ccaccgatgg ggctctgatc gagatcccga agggagccgt gatcgcggac
     4741 cccctggtta tcgagatcca aggcgtgact ccgaacgccg catcgtttcc acatgtgatc
     4801 gttcaagtcg gcgagaacgc agaggcgagt gtcctcgtgg tgtaccgatc agccgacgac
     4861 gccagactgc ttatggtccc cgagatcgat ctccaagtcg ctgcgggggg ccgactccgc
     4921 tacctggcag tccaaggact ggatcaagcc gccacctccg tcgtccatca gcgggtgacg
     4981 ctgagccggg acgccaccgc ccgaatcgga gaggtcgggc ttggcgcaaa gctggggcgt
     5041 ctcgatctgg ggatcgactt gatcggagat ggctcctcgt cggaggtggt cgggctctac
     5101 ttcggggaag gggagcaaac gctggactat cggatggtcg tcactcacca tggcaagagc
     5161 acgacatcgg atgtcttcct caagggagct gtcgaggatg acgctcagag tgtgttcacc
     5221 ggcctgcttc gaatcgagaa ggacgccagg ggcacctcca cattcgagac caatcgcaac
     5281 cttgtcctct ccgagaacgc caaggcgcag tcggtcccca acctcgaaat cctctgtgat
     5341 gacgtggtgt gcgggcacgg ctcgtcggtg gggccgctcg agcaggagca tctctattac
     5401 cttcagagtc gaggtctcac ccgggagcgc gccgagcgac tcttgatcag aggcttcttc
     5461 caggaggtca tcgatcggct tccgatcgag gccgtcggtg gccctgtctc cgacgaggtg
     5521 ttccgacggt tcgtctccgc tcaggaggaa gggcgggtgg catgaagatc gagcttggcg
     5581 ccctggaagc cctcccgcgt gaccggggga tcagagtgac agtcggtgac acccgggttg
     5641 ccgtgtttcg ggttgccgac gaggtattcg ctatcggcga tcggtgctct cacgccgagg
     5701 cctccctggc tgaaggggag gtgtacggca ccgaagtcga gtgccccagg catggctcag
     5761 agttcgagct gcggaccggc gagccgatct ccttgccagc cacacagccg gtcccgacat
     5821 atgaagtcaa gatcgaggac gggaacgtct atctcttcct ggagctgcca tgaccgccgc
     5881 attgaagatc aaggggcttt ctgcctcggt cgccagcaag cagatccttc aggatctcga
     5941 tctcgacgtt ccgtacgggg agatccacgc aatcatgggc cccaacggct cgggcaaatc
     6001 gactttgtgt catgtgctca ccggcaaggc cggctacgag gtatcagggt cggcgaccct
     6061 cgacgatgtc gatctcttct cgttgactgt cgacgagcgt tcccgtctcg ggctcctcca
     6121 gagcttccaa tacccgaccg aagtgccagg ggtcaggctt cgggagtttt tgctcgaagc
     6181 aaccgaggag cgaggcgtgg accccgaggt ggccgccacc cgaatcgagg ccgaggctga
     6241 gcgttttgac acgactcgtt ttctcgatcg gtctctcaac aacgacttgt cgggcggaga
     6301 gaagaagcgc tcggagatct tccagatggc ggtgctccgt cccaagctcg cccttctcga
     6361 cgaaatcgac tccgggctcg acatcgacgc ggtccgccag gtgtccgaag ccgtcgagtc
     6421 gatgcgatca cccgacttgg gggtggtcat gatcacccac tacagccgga tccttcgcta
     6481 tctgatgccc gatcgaatcc atgtgatgat cgacggccgc atcgttgcct ctggtggtgc
     6541 cgaactggcc caggagctcg acaccggggg atacgaatcg gtgcgggatc gcctcggctt
     6601 gcagcgcgcg tcgggcgaac cggtgcgcaa ggcctctgac ttctttaccg acaccccgtt
     6661 cgacgtctga gcccaatggc cgaccgttcg actgccgagc gttggttggc ggctctcacc
     6721 aactctccaa ctgcgtccgg acatgaggat gcagtcatcc gctgggttcg ggcatgggtt
     6781 gcaagacgcg aagacctctc ccttcgcagc gatgctggcg gcaacctctt gatcacgcag
     6841 aagggacgtc ggacgcgatc tccggtggtg gcggtggcgc atatggacca tccggccttc
     6901 gtggtcacgt ctgtggaagg ccgagaggct cggtacgagt tccggggtgg ggtccggcct
     6961 gagtatttcc agaacgctcg ggtggtcttc ccatcccgcg gaccggcgat caaaggcgtg
     7021 gtccagaccc atgaccccaa gaccggcacc ggttcgatcc gggtgaccgg aaacggggcg
     7081 gtcaaccccg gagcgatcgc aacctggggc ttctctgggc aacggacgaa ggccgggcac
     7141 ttctcggctc ctgcgtgcga cgacctcgcc ggatgtgcgg ctggcctggc cgccctcgat
     7201 cgagcacgag ccaccccggc gcttcgtcat ttcggagtgc tgttgacgag ggcggaggag
     7261 gtcggtttcg taggggcaat tcatgccgcc aagaccggca cagtttccag agagacacgg
     7321 ttgctgtcga tcgaggcctc ccgggcttcg gctcatgcgc cggttggagc cggtccagtg
     7381 gtgagggtgg gagatgcctc caccgtgttc gatcgggagc tcaccaacca agtcagctgg
     7441 gccgcggcgc gtaggggtct gccacatcag cggaagttga tggcaggtgg atcatgtgag
     7501 gccacggctt tcggcgttta cggataccgg gcggccgggc tctgtcttcc cctcgagaac
     7561 tatcacaaca tgggaaacct cgacgaggtc gagtccggat cggggaaggc aaccccaatg
     7621 ttggaaacca tcgcactgtc cgacttccac gcgttggttg atctgctctt gctggccgct
     7681 gaagcagtcg acgatgtggg tggcctggag acgaggctcg accatctcta cgaaggcagt
     7741 aggcacctgc tcgaacgcag accgttaccg taagggcgac cctcgaaagg gagcaacgac
     7801 aggcgtggaa cagagaatcg aacgtgactc gatgggcgag gtcgaggttc cggcggacgc
     7861 ttattacggg gcttcgacgc aacgagccgt cgacaacttc cctatttcgg ggcttcgctt
     7921 tggccgtcga ttcatttggg cactcggcct gctcaagggc tcggcggcca gtgtggctgc
     7981 gcagttgggc cacgtggatg ccgaacgcgg cgccgccatt cgcaaggcag ccgacgaggt
     8041 gatggaaggc tcgctcgacg ggcactttgt gctcgacatc ttccagaccg ggtcgggcac
     8101 ttcgaccaac accaatgcca atgaggtgat agccgcccgg gcctccgagt tgctggccac
     8161 cggggcagat ccggtacatc ccaacgatca ggtcaatttc ggtcagtcct caaacgacgt
     8221 gataccggct gcaattcaca tcgcagcagt ggccgctatc cgcgagcagc tgatcccggc
     8281 gctggatcgt ctgagctcgt cgctcgacat caaggcgagg gagtttgccg atgtggtgaa
     8341 gtcggggagg acccacctca tggacgccac cccggtgacc ctcggacagg agttcggagg
     8401 ctgggcaaca caggtcgcca agggatcgga gcggctcgcc aaggtgctgc ccgagttgga
     8461 ggagctggcg ctgggcggaa ccgctgtcgg gaccggcatc aatgcacccg tggggtttgc
     8521 cccggcggtg attgcactca tggccgaccg caccggatat ccgttccgcg aggctgacaa
     8581 ccatttcgag gctcaaggct cgaaggatgc agccgtgatg gcgtcgggcg cggtgaagac
     8641 agttgccgtc tcgctttcca agatcgccaa cgacatccgc tttcttggat cgggccccag
     8701 ctctggcctg gcggagatca ggatccccga gctccaaccg ggctcgtcga tcatgccggg
     8761 gaaggtcaat ccggtgatgt ccgagatggt catgcaggtc gccgctcagg tcattggcaa
     8821 cgacgccacc atcacctggg gtggggcaag cggcaacttc gagctcaatc tgatgatgcc
     8881 ggtgatcgct cacaaccttc tggagtcgat cgagattctg gccaatgccg ccgaggcgtt
     8941 tcgggcaaaa tgtgtcgaag ggatcgaagc cgatcgagag cgggccctcg cactgttgga
     9001 gaggaacgcg atcatcgtca ccgccctcaa cccccacatc ggttacgaca acggggccaa
     9061 gatcgccaaa gagtctctcg cttcaggacg ttcgatacgg gacctcgtcc tggaatcagg
     9121 tctgatgacc gccgatgagc tcgacaaggc cctcgatttg aaaaagctca ccgagggcgg
     9181 gatcgcctag gcgcctccgg cgcccgtttc tccccccagt gagcgaaacg agcggtgggg
     9241 ggagtaccgc cgcaggcggg gaggggggca tcccaacacg catcgacggg gggttagtcg
     9301 ggagaggcga gcgcctcggc caacacgttc cacgccaggc tggcgcattt gatccgcacc
     9361 ggatactggc gcactccttc gagagcctcg aggtcgccaa gggccgcccc tgggtgatcg
     9421 tgatccacac ccggatcgat tccgtcgtcg agtgacatca aaccccggaa cttggcggtc
     9481 agctcttcga tctcggcccg gctcttgccc ttgatcgcct cactcatcat cgaagccgat
     9541 gacaggctga tcgagcaccc ctgaccgtcg atggccaccg acccgaccgt gtcgccgtca
     9601 tacatgagct cgatcgtgat gtcgtcgccg cacagcgggt tgatcccctg cgcctttgcg
     9661 tccggtgcgt cgagatggcc cttgttgcga ggggtccgat agtgatcgag gatcacctcg
     9721 cgatagaggt cttcgaggct cacttgccaa ccccgaacag atcacgagcg tgacgcaggg
     9781 ccagaacaag agcgtctatg tcctcttcgg tgttgtacac gtagaagctg gcccgggccg
     9841 ttgccgccac tcccagcttt tgcataagcg gtcgtgcaca gtgatgcccc gatctcacgg
     9901 acaccccttc ctcgtcgagg attgttgcca ggtcgtgagc gtggacttcg tccatggtca
     9961 tcgagagcac cccgccccgg tcgatggtgt tgggaggtcc ctggatcttg agccccggca
    10021 cgtcaccgag gcgatcgagg gcgacttcgg tgagagcgcg gtcgtgctcg aagatcttgt
    10081 ccattccgat cgagtccaga tagtcgatgg cggcgtggag gccgacggcc tcgatgatcg
    10141 ggggagttcc cgcctcgaaa cggtggggta tctcggccca ggtggaatgt tggagggtga
    10201 cctcggagat catctcccct ccaaactcga agggctcagc ctcctggagg gcgtccattt
    10261 ttccccacag gatcccgatt ccggttgggc ccagcatctt gtgggacgaa aaggccacga
    10321 aatcggcgcc gaggtcgtcg accttgaccg gcgcatgtgg gattagctga gcggcgtcga
    10381 ccaccacccg tgccccttgg gcatgggcga gatccgcgat ctggcgaacc ggcggcatcg
    10441 tgccgagaac gttcgacatc ccggtcacgg ccacaatctt gacgtccggg cccatcttct
    10501 gttggtacgc ctccatgtcg aggcgaaagt cgtcggtcac cgggatgtgg tcgagggtgg
    10561 ctccggtgcg cttggccact atctgccacg ggaccaggtt ggcgtgatgc tccatctcgg
    10621 tggagaggat ccgatcgcct tccttgaggt tctccaatcc ccagccaaag gcgaccgtgt
    10681 tgagcgatgt ggtggcacca cgggtgaaga tgatctctcg tgggtccttt actccgagga
    10741 actccgccac ccggttgcgg gcggcctcat aagcgtccgt ggaggcaacg gagagcttgt
    10801 aagcaccgcg gtggacgttg gcgtaggaag ttcgggcgaa ccgatccatc gcttcgagga
    10861 cggggatcgg cttctgcgat gaggcgcccg agtcgaggaa gtggactcca tcaccgagga
    10921 cgggaaagtc ggctttgaga tgcgatagat cgatcacaac taggtcaaca cactccggaa
    10981 cgaatcgtat ccctcctctt cgagacgggc cgccaactca ggccc
//
LOCUS       DBNHXU010000055         2718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4978113, whole genome shotgun sequence.
ACCESSION   DBNHXU010000055 DBNHXU010000000
VERSION     DBNHXU010000055.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4978113"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="AC161X_01500"
     CDS             complement(<1..589)
                     /locus_tag="AC161X_01500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988255.1"
                     /translation="MSRVFECGCGVAVSGTDIDDLAPQVAAHFDDVHAEMGITPIAVR
                     NYLEAEDRLIGPTERLDSIGSIEIKAIGPDTLNDILGFFDTEAYAGNPAWGSCYCMFF
                     PVGGREAEDIWGSRTASQNRSDQAARIEAGSTTGVLAYVDGKLAGFCNATARAQFPGF
                     ATGNGDQHVGSIVCFTISPPYREHGVAAHLLDGAIS"
     gene            complement(609..1841)
                     /locus_tag="AC161X_01505"
     CDS             complement(609..1841)
                     /locus_tag="AC161X_01505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988256.1"
                     /translation="MAEIKLDGVGKVYPDGTRAVGDLNLTINDGEFLVLVGPSGCGKT
                     TALRMIAGLEEISEGTITIGEKVVNDVAPKDRDIAMVFQNYALYPHMSVFDNMAFGLK
                     LRKFKKEEIDRRVKEAADILEIGEYLERKPKALSGGQRQRVAMGRAIVREPAAFLMDE
                     PLSNLDAKLRVQMRAELERLHTRLGTTTVYVTHDQTEAMTLGDRVAVLRPVSAARPFN
                     LQQVGPPTELFDNPDNLFVAGFIGSPAMNLTYGHLEAEGDQVWAVTPAGFRVKVDPRS
                     LAAHPGIETKMNQEFVIGIRPNSLEAAQVAGPDPDRTITAQVEVTEMLGAETFVHFTV
                     NRPPVVTPDIEELLADTGQDPLSLGDTTNFIARVSPNAQVHKGDSIDLIVDCAKLHFF
                     DPKSGLRVGSKAPVPVEA"
     gene            complement(1893..>2718)
                     /locus_tag="AC161X_01510"
     CDS             complement(1893..>2718)
                     /locus_tag="AC161X_01510"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR4988257.1"
                     /translation="YPHDPVSVGEIHIFDLPEWAAYYGEHLDQIHMPFNFHLMVTPWD
                     AASIRAVIETVLWHVPEGGWANWLLGNHDEVRLMTRLGPLNARLAAMLLLTLRGTPFL
                     YYGDELGMPQSEISPGEGRDPWGENVSYLSRDGCRTPMQWDDSPTAGFGDNGSWLQVD
                     PSFVELNVVAESGDPGSMLALYQRLLAYRRSSAVLREGKYVSHPASNGDVLVFERFTE
                     QERVIVALNLSDQLQLLDLPEGSVVISTNSITTRAVGSEGIFLGRREGVIIEVLVP"
BASE COUNT          525 a          835 c          829 g          529 t
ORIGIN      
        1 acgagatcgc accgtcgagg aggtgagccg ccacgccgtg ttcccgatat ggcggagaga
       61 tggtgaagca gacaatcgag ccgacgtgct ggtcgccgtt cccggtggca aacccgggga
      121 actgagctcg cgcagtcgca ttgcagaacc cggccagctt tccgtcgacg taggcgagga
      181 caccagtggt tgaaccggct tcgatcctgg ctgcctggtc acttcggttc tgcgaagcgg
      241 ttcggcttcc ccagatgtct tcggcttcgc gaccgccgac cgggaagaac atgcaataac
      301 acgaccccca cgccggattc ccggcatagg cctccgtgtc gaagaagcca aggatgtcgt
      361 tgagggtgtc aggcccgatg gccttgatct cgatgcttcc gatcgagtcc agccgctcgg
      421 tgggcccgat cagccgatcc tcggcctcga gatagttgcg aaccgcgatc ggggtgatcc
      481 ccatctcggc atggacatca tcgaaatgag cagccacctg cggggcgagg tcgtcgatgt
      541 cggttcccga gaccgcaacc ccacatccgc actcgaagac ccggctcatg ccggcatcaa
      601 tccgctgcct aggcctcgac cgggaccgga gccttcgacc caacccggag cccggacttg
      661 ggatcgaaga agtggagctt ggcacagtcc acgatgaggt cgatggagtc gcccttgtgc
      721 acctgggcgt tggggctgac ccgcgcaatg aagttggtgg tatccccgag ggacaatgga
      781 tcctgaccgg tgtcggccag tagttcctcg atatcgggcg tcaccacggg tgggcgattc
      841 acagtgaaat ggacgaaggt ctcggccccg agcatctctg ttacctctac ctgggccgtg
      901 atggtcctgt ccggatcggg cccggctacc tgagccgcct cgagagagtt ggggcggatg
      961 ccgatcacga actcctggtt catcttggtc tcgatgccag gatgcgcagc caaggaacga
     1021 gggtcaacct tgaccctgaa ccccgcgggc gtcaccgccc acacctggtc accttcggct
     1081 tcgaggtgac cgtaagtgag gttcatcgcc gggcttccga tgaatccggc gacgaagagg
     1141 ttgtccggat tgtcgaatag ctcggtcggc gggcccactt gctgcaggtt gaaaggccgt
     1201 gccgccgaaa ccgggcggag tacggcaacc cggtcgccga gagtcatcgc ctcggtctgg
     1261 tcgtgggtga catagaccgt ggtggttccc aacctggtgt gaagcctttc cagctcggcc
     1321 ctcatctgga ctctgagctt ggcatcgagg ttggaaagcg gctcgtccat gaggaaggcg
     1381 gcaggttcgc gaacgattgc ccgccccatg gccacccgct gtcgctggcc acccgagagc
     1441 gccttcggct tgcgctcgag gtattcccct atctcgagga tgtcggcggc ctctttcacc
     1501 cgccgatcga tctcttcctt cttgaacttc ctcagcttga gaccgaaggc catgttgtcg
     1561 aagaccgaca tgtggggata gagggcatag ttttggaaga ccatggcgat gtcccggtct
     1621 ttgggagcca cgtcattgac gaccttttcg ccaatggtga tcgtcccttc gctgatctcc
     1681 tcgagaccgg caatcattcg gagcgccgtc gtctttccac agcccgacgg tcccacgagg
     1741 accaggaatt ccccgtcgtt gatcgtcagg ttgaggtcac cgaccgctct ggtgccatcg
     1801 ggatagacct ttccgactcc gtcgagcttg atctcggcca actcatctcc tccaggaatg
     1861 tgttgcttgc gaagatagcc tgatttgcct cgtcacggca ctaaaacctc gatgatcaca
     1921 ccttcacgac gtccgaggaa gatgccctcg ctgccgacag ctcgcgtcgt aatgctgttg
     1981 gtggagatca cgactgagcc ctcgggaagg tccagcagct gaagttggtc gctgagattg
     2041 agagcaacga tgacgcgttc ctgttcggtg aatcgttcga acaccaggac atcgccattg
     2101 gatgcagggt gggagacgta tttgccctcc ctgaggaccg ccgaggaacg gcgataagcc
     2161 aggagtcgct gatagagcgc caacatcgaa cccggatcgc ctgactctgc aacaacattc
     2221 agttcgacaa aggaggggtc gacctggagc catgacccgt tgtcgccgaa cccggccgtc
     2281 ggcgagtcgt cccactgcat cggcgtccga caaccgtccc ggctcagata ggagacgttc
     2341 tcgccccagg gatcacgacc ttctccaggg gaaatctcag actggggcat tcccaattcg
     2401 tcgccgtagt agaggaaagg tgtcccccga agcgtcagga gcagcatcgc cgcaagacga
     2461 gcattgagcg gaccgagacg ggtcatgagc cgaacctcgt cgtggttgcc cagcaaccag
     2521 ttggcccagc ccccttcagg aacgtgccac aacaccgttt cgatgactgc cctgatactc
     2581 gccgcatccc aaggcgtcac catcagatgg aagttgaacg gcatgtggat ctggtcgaga
     2641 tgctcgccgt aatacgccgc ccactcgggc aggtcgaaga tgtggatctc tcccacggac
     2701 accgggtcgt ggggatag
//
LOCUS       DBNHXU010000056        13269 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1604987, whole genome shotgun sequence.
ACCESSION   DBNHXU010000056 DBNHXU010000000
VERSION     DBNHXU010000056.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 13269)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13269)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13269
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1604987"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            120..1262
                     /locus_tag="AC161X_01515"
     CDS             120..1262
                     /locus_tag="AC161X_01515"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein hydrolase activator EnvC family protein"
                     /protein_id="HIR4988258.1"
                     /translation="MTKASRTLAALLVVALALPAYAEVTDEDVDRARDEVDAVLAESE
                     ALGIQVQEAWARQYALDKEISDLETSIDLAHARIADTEARLEDVAVEMYMGSARSASL
                     QVLFSEGSEGFGAGQEYLQEISGADQDVVSQLRAYRSELDRQTGRMAEASAEQETVTA
                     DLEQMAGDLQAELAKAQKVYDRLVGQQQAEEEARRKAEEEARRRAEEAAQNATTTTAG
                     GGGGGNGGTTTTVGGGETPTTTPDPPPTPPSGGTCPVAGAVSFSDSYGAPRSGGRAHR
                     GVDMIAARGTPIVAIYSGTIRRFSNSSLGGLSIYFVSDDGDMFYYAHLDSFADVSAGQ
                     HVDQGRVLGYNGSSGNAPDWLPHLHFEWHPGGGGAVNPYQLVRSLC"
     gene            1728..2150
                     /locus_tag="AC161X_01520"
     CDS             1728..2150
                     /locus_tag="AC161X_01520"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR4988259.1"
                     /translation="MPAGTPSNLADWGIRAIGAIIDWAPVIIISIVTYRIPFFGLLSA
                     AYLAYLGHLEGTTGQTPGKAMQGTRLVNETGELLGSGAGIGRKFLHILDWICFIGFLL
                     PLVDTKRQTFADKIMNSYVVTGIEKKPFNVSLWAAPKA"
     gene            complement(2212..3348)
                     /locus_tag="AC161X_01525"
     CDS             complement(2212..3348)
                     /locus_tag="AC161X_01525"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HIR4988260.1"
                     /translation="MPTRDDVLKTLEGVRDPELGGNVVELGMITDVTVHGSTVDVGLA
                     LTVAECPLRNQIENDTRRRVLSMPGVDELNLRTTAMTKQQRAGLMSVARQRVRENAEP
                     TQVSPTTRVIAIGSGKGGVGKSSIAVNLSVALVGAGFRVGLLDADIWGFSTPRMLGVE
                     DRLHADDATKLIEPVDAQGVRLVSTGLIIESEETALMWRGLMLSKALEQFLKQVDWGE
                     LDYLLIDLPPGTGDIQMALTRLLPQAEMVVVTTPQRAAQKVAVRVADMARRSHMPIVG
                     VIENMAGLACDCGQVHTVFGEGGGQELADDLGVPLIASIPLDPEVVPGGDNGAPVVAS
                     GTDSAAGKALRQAAETLVTLVPPADLETCTGRIAKLLEGLEIGA"
     gene            3449..3811
                     /locus_tag="AC161X_01530"
     CDS             3449..3811
                     /locus_tag="AC161X_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HesB/IscA family protein"
                     /protein_id="HIR4988261.1"
                     /translation="MTQILSIKSSQPQKTVMLTDIAIDKVRELIQAESDTDLALRMAV
                     RPGGCSGFSYEMYFDSQIDASDIVEEFGDVKVVVDPQSAEMVRGATLDYKDGLMETGF
                     SINNPNVSHTCGCGNSFS"
     gene            3870..4241
                     /locus_tag="AC161X_01535"
     CDS             3870..4241
                     /locus_tag="AC161X_01535"
                     /inference="COORDINATES: protein motif:HMM:NF024788.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR4988262.1"
                     /translation="MVDNFWDVILWMLAFFVFCMFFWLFVSVLADLWSDPDTGGGAKA
                     FWTFFVVILPWLGILIYLIARGGGMAERAMKKQQAQEEYIRKVAGSGGSASELAALSD
                     LKAKGDLTQEEFDKAKASLLA"
     gene            4358..4738
                     /locus_tag="AC161X_01540"
     CDS             4358..4738
                     /locus_tag="AC161X_01540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015721517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR4988263.1"
                     /translation="MPKTVPVTPSAPGAMGPYSVVTEANGFVFVSGQVAVDPITKERA
                     PDDVEAQASQVMTNIIAILGDVGLGISDIVKTTIYLADIDDFPKVNAIYSGFLDEEPP
                     ARSTVQAGALPGGFLVEIDVVAAR"
     gene            4735..6249
                     /gene="amaB"
                     /locus_tag="AC161X_01545"
                     /gene_synonym="amaA"
     CDS             4735..6249
                     /gene="amaB"
                     /locus_tag="AC161X_01545"
                     /gene_synonym="amaA"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007416536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-piperidine-6-carboxylate dehydrogenase"
                     /protein_id="HIR4988264.1"
                     /translation="MNKTEIFDRLGVSESNSGVFGGEWLEATGETIEVVNPTTQETLG
                     SVTMGSVADYEKVVANSVETFHRWKLLPAPKRGAYIRSLGDALRDNIDALGALVTLEV
                     GKILPEGIGEVQEMVDICDFAVGLSRQLYGNTMASERPNHRMYEHWHPLGPVGVISAF
                     NFPVAVWSWNSALAAVCGDTVVWKPSERTPLSAIAVTRIAEEVMAGSGYEGVFNLVVG
                     DGATVGSAMNADARLPLISATGSCNMGRIVGPAVAQRMGRTILELGGNNAVIVMDDAD
                     LDLAVRATLFSAVGTAGQRCTSLRRLLVHRAVSDKMIDRLAEAYRQVNIGDPLVPGTL
                     MGPLVSEGAVEQMMNALDIAREQGGRIVTGGNRIDRPGFFVEPTIAVVPADATITQQE
                     TFAPILWVIEFDTLDEAIQIQNGVTQGLSSAIFTESVRNAEKFLSDVGSDCGIANVNI
                     GTSGAEIGGAFGGEKETGGGREAGSDAWKAYMRRQTTTINWGDDLPLAQGIEFG"
     gene            6252..6845
                     /locus_tag="AC161X_01550"
     CDS             6252..6845
                     /locus_tag="AC161X_01550"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4988265.1"
                     /translation="MDATDWDEKYKEAAHLHWPATPNIFVSDRLHDAVPGRGLDLAAG
                     EGRNAIWLASLGWKMTAVDFSEVAIERGSARATDVDFVVADALEWEPDTSFDLILIAY
                     LHLQSAEYEKVVHRAKEWLEPGGELFMIGHDVSNVEHGHGGPQYPEILWNVDEMIGWM
                     EGLTIIEGQVVRRPVETDEGRVFARDALVRGRRPVSA"
     gene            complement(6842..7993)
                     /locus_tag="AC161X_01555"
     CDS             complement(6842..7993)
                     /locus_tag="AC161X_01555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015896165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-sulfuration enzyme family protein"
                     /protein_id="HIR4988266.1"
                     /translation="MGFRTKQIHAGVEPDPTTGAILTPIHQSTTFVQESVDAYMAKGF
                     SYSRAGNPTVRAFEEKMIALEGGVDATAYGSGMAATVAVFLALLRAGDHVIIGDVVYG
                     GTYRFADQFLTRFGVDVSFVDAADPEAVASAVGPTTRMVFTETPANPTLKLTDLEAIS
                     DICSQAGILHVTDNTFLTPYFQRPFELGANVIIHSTTKFLDGHNATLGGVVVVDSADL
                     QEKIAFARIAAGLVMSPMVAWLTLQGVKTLSQRMDGQSANAMAVAEWLAGHPKVDYVN
                     YPGLASHPQHDLAKRQATGFGALVCFEIHGGLEPGKRLMDAVELWSLAENLGSVESLI
                     THPVTMTHAAVPAAERAAAGITDGLVRLSVGLEDAEDLIADLDSALDRI"
     gene            complement(8001..8636)
                     /locus_tag="AC161X_01560"
     CDS             complement(8001..8636)
                     /locus_tag="AC161X_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018218090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HIR4988267.1"
                     /translation="MIVAVHPVECSEGVTDGLALGGATVTPAPELVPEDRRINDSDLL
                     LVELGDQPMRRLRELTRIVETIGLPVIVVASVEHLALMEGAPGISDFLTAPVDPLELR
                     LRLQRLTMAEAVDELVKFKDLELNPLNYQASLAGDPLDMTFMEYELLRFLMENPVRVW
                     SREQLLSKVWGYDYYGGARTVDVHIRRLRAKLGEERSSWITTVRSVGYRFG"
     gene            complement(8633..9958)
                     /locus_tag="AC161X_01565"
     CDS             complement(8633..9958)
                     /locus_tag="AC161X_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR4988268.1"
                     /translation="MNKQAEYVLRTVEERRIRFVRLWFTDVQGFLKSVSIGPTELEVA
                     FEEGMTFDGSSIDGYARIQEADMLAKPDPSTFQVLPWRTEEQVARMFCDIYTPDGEPF
                     SGDPRIVLKRNLEKASEMGYIFYAAPELEFFYFRDSGPVPEVLDRGGYFDLTPLDVAQ
                     EYRRNTINALEHMGIPIEHSHHEVAPSQHEIIVRYTDALTMADNIMTSRLTVKEVALQ
                     HGIYATFMPKPLEDYDGSGMHLHLSLFEGDVNAFHEPGTPGGLSKVGQAFIAGLLRHA
                     AELTAVTNQWVNSYKRLVAGFDAPIYVSWARNNQSALVRVPSVKKNKPSSVRVEYRAA
                     DAACNPYLALSVILAAGLEGIREGYELPAEVAADIHRLTPAERAAAGVKRLPETLSEA
                     LDTLEQSELMAEALGEHVFDWFLRNKRREWDRFQHHVSQFELETYLPVL"
     gene            complement(9987..11123)
                     /locus_tag="AC161X_01570"
     CDS             complement(9987..11123)
                     /locus_tag="AC161X_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4988269.1"
                     /translation="MALIFLANGAVVGSWIPRLPEIRDRLSMDLDTLGLTLALGGLGA
                     LLGSSVSGVLLGRFGARRSAVVGSVVLFGLLPLIAVVPTPFLFGTLLAILGFVDAQTD
                     VGMNAVGVRVEESVGRSIMTRLHGLWSLGTLVGSAFAAFAVLVGVGLGPQLLALSAAG
                     LVVIAFAARLVPDSTPRRGTGERSGRIAIGLILAGATAVMIESAPMDWGAIFLIDITG
                     ASGAVVGTAAIVFTAGMLVGRMAGDHVVDRFHGVPTVVAGILVSASAMTLVVFTESTP
                     MALVGFGIWGLGISVVLPVLYKLAGSHPTFAEGAGLAALTVGTRLGFMAAPALIGFVA
                     ARSSLPTALGVVVGLAAAASLVTIRMTLSGGDQIRDSLPSRGAS"
     gene            complement(11172..11492)
                     /locus_tag="AC161X_01575"
     CDS             complement(11172..11492)
                     /locus_tag="AC161X_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR4988270.1"
                     /translation="MANRGNATKLRGVFIGGPELSPEVVADWRDQAACSGLPHSVFFP
                     VGDDLEVAITNAKRICAICPVTDDCLEFALETNEKAGIWGGTSEEDRKALRRKWLATR
                     RRVG"
     gene            complement(11513..12499)
                     /locus_tag="AC161X_01580"
     CDS             complement(11513..12499)
                     /locus_tag="AC161X_01580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988271.1"
                     /translation="MPNGSESQSSGTEKVKVQTRARPWSGLLLGMLLGISLAVILQQA
                     GVWPLDRLLLFGFAGLFGLIGILLTGWGRESASGFSSVVPLVLAVAMVGWGATGLTQL
                     DEHGELNGGCTVEAQSDVDSTIMTDTTRQEPFEVDPEGGLSWSATSPAPITDHTWEIW
                     VDVGGFQVVVAEGSDSNADQNLENSGDVPDISVYVEEVTSISGQQIRGVVEVGGSIDG
                     DGGACDGFAFVRLTADFFDTLISRIAAALALISLIALITITARRTRMADVVPEAAATS
                     AGAAAANQPMDDATDEASTAVETETQTDGGAMGAAKPDDTPPDDDEGFPPTT"
     gene            12600..13217
                     /locus_tag="AC161X_01585"
     CDS             12600..13217
                     /locus_tag="AC161X_01585"
                     /inference="COORDINATES: protein motif:HMM:NF036525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR4988272.1"
                     /translation="MPRIRAATIDEHKALTRSALLNAAKTLIREAGTADLPLGEIALA
                     AGVGRTTLYDYFVDRDDLIATLVEDELPGVVDALITAVPHGGSASERLADLAARTVEF
                     VISDPVLGVILHREVGRMGPEAEVRIRAAHATLADAMVGLYHAGVESGDFRSMPPDLA
                     GRLIQDSIMSAARTVLSAKSPQDRIESVTMHLRLFLLGGFGVGRL"
BASE COUNT         2470 a         4155 c         4205 g         2439 t
ORIGIN      
        1 ggagcacccc tccaggcact cgccctgcgc tcggtccagg ctcacctcac tttgctcacc
       61 gaccagggac ggatagcctt cgagcacggc gagcacgtcg tcgcgtcccc ggaatcccct
      121 tgaccaaagc atcccgcacc ctcgctgcct tactcgtcgt ggccctggcc ctgcctgcct
      181 atgccgaggt cactgacgag gacgtcgatc gtgcccggga cgaggtggac gcggttctcg
      241 ccgagtcgga ggcccttggg atccaggttc aggaggcatg ggcccggcaa tacgccctcg
      301 acaaggagat atctgacctc gagacttcga tcgatctggc tcacgcccga attgccgaca
      361 ctgaggcacg cttggaggat gtagcggtcg agatgtacat gggatcggct cgctctgcct
      421 cgcttcaggt cctcttctcc gaggggagcg agggcttcgg ggcgggtcag gagtacctcc
      481 aggagatctc tggagccgac caggacgtcg ttagccagct tcgtgcctac cgatccgagc
      541 tcgatcgtca gaccgggcgc atggctgaag catcggcgga gcaggagacg gtcaccgcgg
      601 acctggaaca gatggcggga gacctccagg ccgagctggc caaggcacag aaggtctatg
      661 accgtttggt gggccaacag caggccgagg aggaggcccg gagaaaggct gaagaagagg
      721 ctcgccgtcg cgccgaggag gctgctcaaa atgccaccac cacgaccgcg ggcggtggcg
      781 gtggcggcaa tggaggcacc acgaccacgg tgggtggtgg cgagacgccg accacgacac
      841 cagacccgcc gccgacgccg ccgagtgggg gcacctgccc ggtggcgggt gcggtgtctt
      901 tctctgactc ctacggggca ccgcgctcag gcggccgcgc tcatcgtggc gttgacatga
      961 tcgcggccag ggggacgccg atcgtcgcca tctactcagg cacgatcagg agattttcca
     1021 acagcagcct gggcggtctg tccatctact tcgtgtcgga cgatggggac atgttctatt
     1081 acgcccatct cgacagtttc gccgatgttt ccgctggtca gcacgtcgac cagggtcgag
     1141 tgctcggcta caacgggtcg tcggggaacg ctccggattg gcttccccat ctccacttcg
     1201 agtggcatcc ggggggtggt ggggcggtca acccctacca attggtcaga tcgctgtgct
     1261 gaaggcacgg aaaaagggcc cttctgggcc ctctttccga ggcctccctc tccccaaccc
     1321 caacagccca cttggcacgt tcctcacata tcattgacca cggagagtgg ttaggtaagg
     1381 acgattccac agtcacccat ctggactagt ccgtcgacga cccggtggat cgccagctaa
     1441 ctttgatcct tgccgacaca tcgcgatgcc aaatggcaca atgcgaaaat ccgatccgag
     1501 gagagaatgt cttgagcacc caagagcccc cggctccccc acctccctac gagccgccga
     1561 ctcctccacc acccccggaa cccccggctt cgcctcctcc ccctccgccg ccaccttccg
     1621 agcctccacc gcctccctcc ccgccaccat ctgagccgcc ggtctcaccg ccgccacctc
     1681 cgcctcccgc gccgccagcc gtgcctcccg catctccgcc acccgcggtg cccgcgggga
     1741 cgccatcgaa tctggccgac tgggggatcc gtgccatcgg ggcgatcatc gactgggctc
     1801 cggtgatcat tatcagcatc gtgacctacc ggattccctt ctttgggctg ctttcagcgg
     1861 catacttggc ttacctcggc cacctggaag gcaccaccgg tcagactccg ggcaaggcga
     1921 tgcagggaac caggctggtc aacgagaccg gcgagcttct tgggagtggg gctggaatcg
     1981 gtcgtaaatt cctccacatc ctcgactgga tctgcttcat cgggttcctg ctacccctcg
     2041 tggacacgaa gcggcagaca ttcgccgaca agatcatgaa cagttacgtg gtgactggta
     2101 tcgagaagaa gccgttcaac gtcagcctct gggcggcccc gaaggcgtaa gcgtgccttt
     2161 cggcacactc gtgatacgta gtacgtgtta cgcaggacgt ggagatcggc gctaggcgcc
     2221 gatctccaat ccttcaagga gcttggcgat gcggccggtg caggtttcga gatcggctgg
     2281 cggcaccagc gtgaccaggg tctctgcggc ttgacgaagg gctttgccgg cggctgagtc
     2341 cgtccccgac gccaccaccg gcgctccgtt gtcaccaccc ggcaccactt cggggtcgag
     2401 tgggatcgaa gcgatcagag gcacccccag gtcgtcggcg agctcctggc cgccgccctc
     2461 gccaaacact gtgtggacct ggccgcaatc acaggcaaga ccggccatgt tctcgatcac
     2521 tccaacaatc ggcatgtgcg agcgccgggc catgtcggca acccggaccg cgactttctg
     2581 tgccgcccgt tgtggggtgg tcacgacaac catctccgct tgtggaagca atcgggtgag
     2641 tgccatctga atgtcgcctg ttccaggggg aaggtcgatc aggaggtagt cgagctcacc
     2701 ccagtcgacc tgcttgagaa attgctcgag cgccttggaa agcatgaggc cccgccacat
     2761 gagggcggtc tcctcagatt cgatgatcag cccggttgat accagtctca cgccttgggc
     2821 atcaaccggc tcgatgagct tggtggcgtc atcggcgtgt agacggtcct cgacacccaa
     2881 catgcgggga gtggaaaagc cccagatatc ggcgtcgagc aggccaaccc ggaatccggc
     2941 gcccaccagg gccaccgaca ggttcaccgc aatcgacgac ttcccgaccc cgcccttgcc
     3001 cgagccgatc gcgatgaccc gggttgtggg ggagacctgg gttggctcgg cgttctcgcg
     3061 gactctctgg cgggcaactg acatcagccc tgctctctgc tgcttggtca tcgcggtggt
     3121 ccgcagattg agttcatcca cgcccggcat cgagagcacc cggcggcggg tgtcattctc
     3181 gatttgattg cgcagcggac actcggcgac ggtcagcgcc agaccgacat cgacggtgga
     3241 tccatggacc gtcacgtcgg tgatcattcc caactcgact acgttgccgc ccaactcggg
     3301 atcgcgcacc ccttccaggg tcttgaggac gtcatcacgg gtcggcatca accctcatgg
     3361 taagaggaga gacctggata gtggcattgc cggcacgcca tgctggtcca gatgtgaagg
     3421 cgggttacaa tgagggcagg ttcaacaaat gacacaaatt ctcagcatca aatccagtca
     3481 gccacagaag accgtcatgt tgacggacat cgcgatcgac aaggtccgtg agctcatcca
     3541 ggccgagtcc gacacggatc tcgcccttcg gatggcagtg cgcccaggcg gttgcagcgg
     3601 attctcttac gagatgtact tcgactccca aatcgatgcc tccgacatcg tggaggagtt
     3661 cggcgatgtg aaagttgtcg tcgacccaca gagcgccgag atggtgcggg gcgccacgct
     3721 cgactacaag gatggtctga tggagaccgg gttctccatc aacaacccga acgtctccca
     3781 cacctgcggg tgtggcaact ccttctcctg acaaaccctc cttttcagcg gtgtagggtt
     3841 tctcaagttc atcgaatcag gaggagtaat tggtcgataa tttctgggac gtaatccttt
     3901 ggatgttggc gttcttcgtg ttctgtatgt tcttctggct cttcgtctcg gtccttgcag
     3961 acctttggag cgacccggac accggtggtg gggctaaggc gttctggacg ttcttcgtgg
     4021 tgatcctgcc ctggctgggc atcctcatct atctgatcgc gcgtggcggc gggatggccg
     4081 agcgtgcgat gaagaagcag caggctcagg aggaatacat ccgcaaggtt gccggtagcg
     4141 gcggctcggc gtccgagctc gctgctctgt ccgacttgaa ggcaaagggt gacctaaccc
     4201 aagaggagtt cgacaaggcg aaggcctccc tcctcgcctg agcagaatcc gctaacgaaa
     4261 tcgaagggcc cgggtttccc cgggcccttc tttgtttcaa gactgacagc tccgacgttc
     4321 gggtaccctc ctgacttccc ccgaaacagg agcagcggtg ccaaaaaccg tcccggtcac
     4381 cccgtccgcg ccaggcgcta tgggccctta ctcggttgtc accgaggcca acgggttcgt
     4441 tttcgtgtca ggccaggtcg cagtggaccc aatcaccaaa gagcgagcgc cagatgatgt
     4501 cgaggcgcag gcgagccagg tcatgaccaa catcatcgcg attctcggtg acgtcgggct
     4561 cggcatctcc gacatcgtga agaccacgat ctacctggcc gacatcgatg atttccccaa
     4621 ggtgaatgcc atctactccg gcttcctcga cgaagaaccg ccggcgcgct ccactgttca
     4681 ggcgggtgcc ctccccggcg ggttcttggt cgagatcgac gtggtggcgg cgcgatgaac
     4741 aagaccgaga tcttcgaccg actcggagtt tccgaatcca actctggtgt ttttggcggc
     4801 gagtggctcg aggcgaccgg cgagacaatc gaggtggtca acccgacgac tcaggagact
     4861 ctgggctcgg tcacgatggg gtccgtcgct gactacgaga aggttgttgc caactctgtc
     4921 gagaccttcc atcggtggaa gttgctcccc gcccccaagc ggggcgcata catccggagt
     4981 ctcggtgacg cccttcgtga caacatcgac gcgctgggcg ccctggtcac cctggaggtg
     5041 ggcaagatcc ttcccgaagg gattggcgag gtccaagaga tggtcgacat ctgcgacttt
     5101 gctgtcggtc tctcccgcca gctctatggc aacacaatgg cctccgagag gcccaaccac
     5161 cggatgtacg agcactggca cccgctgggg ccagtcggcg tcatttcggc gttcaacttc
     5221 ccggtcgcgg tgtggtcatg gaactccgcc ctcgctgcgg tttgtggtga cacggtggtc
     5281 tggaagccct cggagcgcac gccgctgagt gcgattgcag tgactcgaat cgccgaggag
     5341 gtcatggcgg gaagtggcta cgagggagtc ttcaacctcg tggtcgggga cggtgccact
     5401 gtcgggagcg cgatgaacgc tgacgcccgt ctgcccctga tatcggctac cgggtcatgc
     5461 aacatgggtc ggatcgtcgg tcccgccgtc gcgcagagga tggggcgcac cattctcgag
     5521 ttgggtggca ataacgcggt gatcgtcatg gatgacgccg acctcgatct ggcggttaga
     5581 gcgaccctgt tctcagcagt cggcacggcc ggtcaacgct gcacctcgct tcgtcgcctg
     5641 ctggtccacc gtgcggtttc agacaagatg atcgatcgcc tggcggaggc ctatcgccag
     5701 gtgaacattg gcgacccact ggtcccgggg accctgatgg gaccgttggt cagcgaaggg
     5761 gcggtcgagc aaatgatgaa tgctctggac atcgcccggg agcaaggagg ccggatcgtc
     5821 actggaggca accgcatcga tcgtccgggg ttcttcgtgg aacccaccat cgctgtggtc
     5881 ccggccgacg ccaccatcac ccagcaggag acttttgccc cgatcctgtg ggtcatcgag
     5941 ttcgacactc tggacgaggc gattcagatc cagaacggtg tcacccaggg cctttcctcg
     6001 gcgattttca ccgagtcggt tcggaacgcc gagaaattcc tcagcgacgt cggatccgac
     6061 tgcgggattg ccaacgtcaa catcggcact tccggggcag agataggcgg ggccttcggc
     6121 ggggagaagg agaccggtgg cggcagagag gccgggtcgg acgcctggaa ggcgtatatg
     6181 aggcgtcaga ccacaaccat caactggggg gacgacctgc ccttggccca ggggatcgag
     6241 ttcggctgag catggacgcc accgattggg acgagaagta caaggaggcc gcacacctcc
     6301 attggcccgc aacgccgaat atcttcgttt ctgaccggct tcacgacgct gtgccgggta
     6361 ggggcctcga cctggccgcc ggtgagggac gcaacgccat ttggctcgct tcgctgggct
     6421 ggaagatgac ggcggtcgac ttctccgagg tggcgatcga acgcgggtcg gcccgggcga
     6481 ccgacgtcga cttcgtagtc gctgacgctc tggaatggga accggatacc agtttcgatt
     6541 tgattctgat tgcctacctt catctccagt cggcggagta tgagaaggtc gtgcatcgtg
     6601 ccaaggaatg gctcgagccg ggtggcgagc tattcatgat cggacacgat gtgagcaatg
     6661 tcgagcacgg gcacggcggg ccccagtacc cggagatcct ctggaatgtc gacgagatga
     6721 ttggttggat ggaaggcctg accatcatcg aaggccaggt ggtgcggcgt cccgttgaga
     6781 ccgatgaggg ccgggtgttt gcccgggacg ccctggtcag gggtcgtcgg ccggtgtccg
     6841 cctagatccg atccagggcg gaatcgaggt cggcgatcag gtcctcagcg tcctccaggc
     6901 ccaccgagag cctcaccaga ccgtctgtga tcccggccgc ggcgcgttcc gcagctggca
     6961 cggcggcatg ggtcatcgtc accggatgag tgatcagcga ctcgaccgag cccaggttct
     7021 cggcgagact ccacaactcc acggcatcca tcagcctctt gcctggctcc agcccaccgt
     7081 ggatttcgaa gcacaccagg gctccgaagc ccgtggcctg ccgctttgcg aggtcgtgtt
     7141 gggggtgcga ggcaagcccg ggatagttga cgtagtcgac cttggggtgc ccggcgagcc
     7201 actcggcgac cgccatcgca ttcgccgatt ggccgtccat ccgctggctg agtgtcttga
     7261 ccccctggag ggtgagccag gcgaccatcg gtgacatgac gagtccggcg gcgattcggg
     7321 cgaaggcgat tttctcctgc aggtcggcgc tatcgacaac aacgacgcct cccagggtgg
     7381 cgttgtgccc atcgagaaac ttggtcgtcg agtgaatgat cacattggcc ccgagctcga
     7441 acggcctctg aaaatagggg gtgaggaatg tgttgtccgt cacgtgcaag atcccggcct
     7501 gagagcagat gtccgagatc gcctcgaggt cggtgagctt cagagtcggg tttgccgggg
     7561 tttcggtgaa caccatccgg gtcgtcggcc ctacggcaga ggccactgcc tctggatcag
     7621 cagcatcgac gaaggagaca tcgactccga accgggtgag gaactggtcg gcgaatcggt
     7681 aggtgccgcc gtatacgacg tcgccgatga tcacatggtc accggcccga agcagagcga
     7741 ggaacacggc gacggtggcc gccatcccgg agccgtatgc agtcgcatcg accccgcctt
     7801 cgagagcgat catcttttcc tcgaaggcgc ggacggtggg attcccggcc ctgctgtagg
     7861 agaagccttt ggccatgtag gcatcgaccg actcctggac aaaggtggtc gactggtgga
     7921 tgggagtgag gatggctccc gtagtcggat cgggctcgac gccggcgtgt atctgcttgg
     7981 tgcgaaatcc catgtcgctc ctagccaaac cggtatccga ccgagcgcac ggttgtgatc
     8041 cacgaagacc gctcctcgcc gagctttgcc cggaggcgcc tgatgtggac atcgacggtg
     8101 cgagcccctc cgtagtagtc gtagccccac accttcgaga ggagttgctc ccgtgaccac
     8161 acccgcaccg ggttctccat gagaaagcgg agcagctcgt actccatgaa ggtcatatcc
     8221 agcggatcgc cggccaggct cgcctggtag ttgagcggat tgagctcgag atccttgaac
     8281 ttgaccaatt cgtcgaccgc ttcggccatc gtgagccgtt gcagccggag gcgaagttcc
     8341 agagggtcca ccggagcggt gaggaagtcc gagatccctg gtgcgccctc catcagagcc
     8401 aggtgctcga ccgaggccac cacgatcaca gggagcccga tcgtctcgac gatgcgtgtc
     8461 agctcgcgaa gtcgtcgcat gggttggtca cctagctcga ccaagagcag gtcggagtcg
     8521 ttgatccgcc gatcttcggg caccaactcc ggggccgggg tgacggtggc gcctcccagg
     8581 gccagcccat cagtcacgcc ttcggagcac tcgacggggt ggacggcgac gatcacagaa
     8641 ctgggaggta ggtctcgagc tcgaattggg aaacatgatg ttggaagcga tcccactcgc
     8701 ggcgcttgtt gcggagaaac cagtcgaaga catgctctcc gagggcctcg gccatgagtt
     8761 cggactgttc cagcgtgtca agagcctcgg acagagtctc gggaagccgt ttgacgccgg
     8821 cggcggcccg ctcggcgggc gtgagccggt ggatgtcggc ggccacctcc gccggcaact
     8881 cgtagccctc ccggatcccc tctagaccgg cagcgaggat caccgagagt gccagatagg
     8941 ggttgcaagc tgcatcggcc gcccggtact cgacccgcac gctcgacggc ttgttcttct
     9001 tgaccgatgg cacccgaacc agcgccgact ggttgttgcg ggcccaggag acatatatcg
     9061 gggcgtcgaa accagcgacc aggcgcttat aggagttgac ccattgattg gtgacggcgg
     9121 tcagttcggc ggcgtgccgg agcagaccgg cgatgaacgc ctggccgacc ttggagaggc
     9181 caccgggtgt ccccggctca tggaatgcat tgacgtcgcc ttcgaacagc gaaaggtgca
     9241 gatgcatccc ggagccgtcg tagtcctcca acggcttggg catgaaggtg gcgtagatcc
     9301 cgtgttggag ggcaacttct ttgacagtga gccgcgaggt catgatgttg tccgccatcg
     9361 tcaacgcgtc cgtgtagcga acaatgattt cgtgctggct cggcgccacc tcatggtggg
     9421 agtgctcgat ggggattccc atgtgctcga gggcattgat cgtgttgcga cgatattcct
     9481 gggccacgtc gagcggggtc aggtcaaagt agccgccccg atcgaggacc tcgggaaccg
     9541 gcccggagtc tcggaagtag aagaactcca gctcgggtgc ggcgtagaag atgtacccca
     9601 tttccgatgc cttctccagg ttccgcttga ggacgatccg gggatcgccg gagaacggct
     9661 cgccgtcggg ggtgtagatg tcgcagaaca tccgtgccac ctgctcctcg gtgcgccaag
     9721 gaagcacctg gaacgtcgat gggtcgggtt tggccagcat gtcggcttcc tggatgcggg
     9781 cgtagccatc aatcgacgag ccgtcgaagg tcatcccctc ttcgaaggca acctccaact
     9841 cggtgggccc gattgagacc gacttcaaga atccttgtac gtcggtgaac cacagccgta
     9901 cgaagcggat gcgccgctcc tcgactgttc gtagcacgta ttcggcctgt ttgttcatgc
     9961 tgagttcccg tccggctggc cccacattat gaggcgcccc ggctcgggag ggaatcgcgg
    10021 atttggtcac caccagaaag ggtcatccgg atggtgacca gcgaggccgc ggctgccagg
    10081 ccgacgacca cacccagggc agtcgggagg ctcgaccgcg ctgcaacgaa gccgatcaag
    10141 gctggagccg ccatgaagcc gagtcgggtt ccgaccgtga gcgccgccag cccggcacct
    10201 tcggcgaacg ttgggtggga cccggcaagc ttgtagagaa ccggaagcac cacggagatt
    10261 ccaagccccc agatcccgaa cccgaccagc gccatcggtg tcgactcagt gaacacgacc
    10321 aacgtcatgg cggaggccga gaccaggatc ccggcgacga cggtggggac accgtgaaac
    10381 cggtcgacca catggtcgcc ggccatccgc cccacgagca ttccagctgt gaagacgatg
    10441 gctgcggtgc cgacaacagc cccggaagca ccggtgatgt cgatcaggaa gatggcgccc
    10501 cagtccatgg gggcactttc gatcatcacc gcggtggcgc cagcgaggat caggccgatc
    10561 gcgatccttc ctgatctctc acccgtgccc cgtcgggggg tgctgtccgg caccaagcgt
    10621 gctgcgaacg cgatgaccac gagacccgcg gccgaaaggg ccaagagctg cggcccgagc
    10681 cccactccga ccaggacggc gaaggcggcg aaggcggacc cgaccagggt gcccagactc
    10741 cacagaccgt ggagtcgggt catgatcgag cgaccgacgg actcttcgac cctgaccccc
    10801 accgcattca tgccgacatc ggtctgagcg tcgacaaacc cgagtatcgc caggagcgtc
    10861 ccgaagagga acggagtggg cacgacggca atgagtggaa gcaggccgaa gagcaccacg
    10921 gacccaacca cggcggaccg cctggctccg aagcgaccca aaaggacgcc actcacggag
    10981 gacccgagga gagccccgag gccacccagg gcgagagtca ggcccagggt gtcgagatcc
    11041 atggatagtc gatccctgat ttcggggagg cgtgggatcc agctgccaac gaccgcgccg
    11101 ttggccagaa atatgagtgc cacgccggtg gtggcgcgct tgaccggcgt cagagcggtg
    11161 cccccgattc ctcaacccac ccgacggcga gtggccagcc acttccggcg gagagccttg
    11221 cggtcctctt cggacgtccc gccccagatc cctgccttct cattcgtctc gagggcgaat
    11281 tccaggcagt cgtcggtcac agggcagatg gcacagattc tcttggcgtt tgtgatcgcg
    11341 acttcgaggt cgtcgcccac cgggaagaag accgaatggg gcaatccaga gcacgcagct
    11401 tggtcacgcc agtcagccac cacctcgggg ctcaactcgg gaccaccaat gaacactccc
    11461 cggagtttgg tggcgttacc ccggtttgcc aagacggctg atcttgttgg agtcaggtcg
    11521 tgggtgggaa cccttcgtcg tcatcgggcg gagtgtcgtc gggtttcgcc gctcccatgg
    11581 ccccgccatc ggtctgagtc tcggtttcga ctgcggtcga cgcctcatcg gtcgcatcat
    11641 ccattggctg gttggctgcc gcggctccgg cagaggtggc tgccgcttca ggcacgacgt
    11701 ccgccatccg ggtgcgccgc gctgtaatcg tgatcagagc gatgagggag atgagtgcca
    11761 gggcggcggc tatccgcgag atcagggtgt cgaagaagtc ggcggtgagc cggacaaacg
    11821 caaagccatc gcatgcgccc ccatcaccgt cgatgctccc accgacctcg accactccac
    11881 gaatctgttg gccggatatc gaggtcacct cctcgacgta taccgagatg tcgggaacgt
    11941 cgccagaatt ctcgaggttt tggtcggcgt tggagtcact cccttcggcg accacaacct
    12001 ggaacccacc gacatccacc caaatctccc aggtgtgatc cgtgatcggg gcgggagatg
    12061 ttgcggacca cgagaggcca ccttctggat cgacctcgaa tggctcctgg cgggtggtgt
    12121 cggtcatgat cgtgctgtcg acgtctgact gggcttcgac cgtgcaaccc ccattgagct
    12181 ctccgtgctc gtccagctgg gttaggccgg tggcacccca tccgaccatt gccactgcga
    12241 ggactagagg taccaccgag gagaatccgg aggcgctctc tcgaccccaa cccgtgagca
    12301 gaattccgat caatccgaac aatccggcga aaccgaatag aaggagtcga tccaagggcc
    12361 agactccggc ctgttgcagg atcaccgcca gagaaatgcc gaggagcatg ccgagtagca
    12421 agccgctcca gggtcgggcc cgggtctgaa cctttacctt ctcggttccg ctcgattggc
    12481 tctctgatcc gttgggcatg gtgttcctcc tggaccacga atctacgccg cttgattccg
    12541 tcctaggcgg caactcttgt ggccgttgcc gcgaagtcgg gatagccttt cgtttctcga
    12601 tgccacgtat tcgcgcagcc acgatcgacg aacacaaggc gctcaccagg agcgctctgc
    12661 tcaacgcggc aaaaaccctc attcgcgaag caggcaccgc cgatctcccg cttggcgaga
    12721 tcgcactcgc cgcgggcgtc gggcgaacca cgctctatga ctatttcgta gatcgcgatg
    12781 acctcatcgc caccctggtc gaggacgagc ttcccggtgt ggtcgatgct ttgatcacgg
    12841 ctgtgcctca cggtggcagc gcttcggagc ggctggccga cctcgccgcc cggaccgtcg
    12901 aattcgttat cagcgacccc gtgctcggcg tgatcctgca tcgagaggtg ggtcgtatgg
    12961 gaccggaggc cgaggtcagg atccgcgccg cccacgccac gctggccgat gccatggtcg
    13021 ggctctatca cgccggagtg gaatcgggtg attttcgatc gatgccaccc gatctcgccg
    13081 ggcggctcat tcaggattcg atcatgtcgg ctgctcggac cgtgctttct gcgaaatcgc
    13141 cgcaagaccg aatcgagtcg gtcaccatgc atctgcgact ttttctactt ggcggtttcg
    13201 gggtaggccg cctctaaccg ataacagtgc cgtggtattc atcagcaccg aagtggagca
    13261 gttcgatcc
//
LOCUS       DBNHXU010000057         1653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4978297, whole genome shotgun sequence.
ACCESSION   DBNHXU010000057 DBNHXU010000000
VERSION     DBNHXU010000057.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1653
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4978297"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..422
                     /locus_tag="AC161X_01590"
     CDS             <1..422
                     /locus_tag="AC161X_01590"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF024897.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HIR4988273.1"
                     /translation="AKGAPVLGICNGFQVLCEAGMLPGALIANRDLAFICRFVEVEVT
                     DVSTILTRSAEIGQRIRIPLNSYEGNWVDPGSGARMVLRYVDNPNGSQGSAAAISNAA
                     GNVVGVMPHPERASDELLGSADGLVLLESFLHSLVPA"
     gene            419..>1653
                     /gene="purL"
                     /locus_tag="AC161X_01595"
     CDS             419..>1653
                     /gene="purL"
                     /locus_tag="AC161X_01595"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HIR4988274.1"
                     /translation="MTITGQPVHRRLGMTDEEYESVVGILGRDPLPAELAMYSVMWSE
                     HCSYKSSRAHLRRFPTQAPWVVVGPGENAGVVDLGDGWLAALRIESHNHPSFVEPYQG
                     AATGVGGILRDVFTMGARPIALWDQIRFGPLDDPKNRYLFGGVVAGIAGYGNAVGVPT
                     VGGEIEFDPTYAGNPLVNVMCLGILRQEQLVLGTAGEPGNVAVLLGKATGRDGIGGAS
                     VLASASFDDGSQEKRPSVQVGDPFEEKKLIEACLEIFEAGLVAGIQDLGAAGLACAIS
                     EPAGRAGVGMDVDLDSVHVRELGMTAPELLMSESQERMMAFVTPANVESVLAIADKWE
                     IDSAVVGTVKAGGTLTVRHGGETVAELPAASLADDAPLYDRPYSRPDWLDDLWEIPEP
                     SSIEPVDALLQLLDDPAIGS"
BASE COUNT          264 a          534 c          560 g          295 t
ORIGIN      
        1 ccgccaaggg tgccccggtt cttgggatct gcaatgggtt ccaggtgctc tgtgaggcgg
       61 ggatgcttcc cggcgccctg attgccaatc gggacctggc cttcatctgc cgcttcgtcg
      121 aggtcgaagt caccgacgtc tcaacgatcc tgacccgcag cgccgagatc gggcagcgga
      181 tccggatccc gctcaactca tacgagggca actgggtcga tcccggctcc ggggcacgga
      241 tggtgctgcg ctatgtcgac aaccccaacg gatcgcaagg gtcggcagcg gcgatttcca
      301 acgcggcagg caacgtggtc ggagtcatgc cccaccctga acgggcctcc gacgaattgc
      361 tgggttcggc cgacgggctg gtcttattgg agagcttcct ccactcgctg gtgcccgcgt
      421 gacgatcacc ggccagccgg tccaccgccg gctcggcatg accgacgagg aatacgagtc
      481 cgtcgtcgga atcctgggtc gggacccgct ccccgcagag ctcgcgatgt attcggtgat
      541 gtggtccgag cactgctcct acaagtcctc gagggcgcac ctccgcaggt tcccaaccca
      601 ggccccatgg gtggtggtgg gtcccggaga gaacgccggt gtggtcgacc ttggtgacgg
      661 ctggctggcc gcgctacgca tcgagagcca caaccacccc tctttcgtcg agccctatca
      721 gggcgccgcc accggtgtcg gggggatcct gcgcgatgtc ttcaccatgg gcgcccgacc
      781 gatcgccctc tgggaccaga tcaggttcgg cccgttggac gatcccaaga accgctatct
      841 gttcgggggc gttgtggccg gaatcgccgg ctacggcaac gccgtcgggg tgccaaccgt
      901 tggtggcgag atcgagtttg atcccactta cgcgggcaat ccgctggtca acgtcatgtg
      961 cctggggatc cttcgccagg agcaactggt gctgggcacc gcaggtgaac cgggcaacgt
     1021 ggctgtgctc ctcggcaagg cgaccgggcg agacgggatc ggcggcgcct cggtgctggc
     1081 ttcggcttcc ttcgacgatg gctcccagga gaagcgaccc agcgtccagg tgggcgaccc
     1141 gttcgaggag aagaagctga tagaagcctg tctcgagatc ttcgaagccg ggttggtggc
     1201 ggggatccag gacctgggcg cggccgggct cgcctgcgcc atctccgagc ctgcgggcag
     1261 ggcgggggtc gggatggacg tcgatctcga ctcggtgcac gtccgcgagc tggggatgac
     1321 cgctccggag ctcctgatgt cggaatcgca ggagcggatg atggccttcg tcaccccggc
     1381 gaacgtggaa tcagtcctgg cgatagccga caagtgggag atcgattcag ccgttgtcgg
     1441 cacggtcaag gccggcggaa ccctcaccgt ccgtcatggt ggcgaaaccg tcgccgagct
     1501 gccggcagct tcgctggccg acgacgctcc gctctacgac cgtccctaca gccgccccga
     1561 ctggctcgac gacctttggg agattccgga gccgtcatcg atcgaaccgg ttgatgccct
     1621 gctccagctt cttgacgacc cggcgatcgg ttc
//
LOCUS       DBNHXU010000058         3498 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4582261, whole genome shotgun sequence.
ACCESSION   DBNHXU010000058 DBNHXU010000000
VERSION     DBNHXU010000058.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3498)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3498
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4582261"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="AC161X_01600"
     CDS             complement(<1..216)
                     /locus_tag="AC161X_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988275.1"
                     /translation="MTEPTPRQVLYGLVAGGFFVVVLALVVGAGVTGVVPLPWTLTIG
                     ALWLILAVWSAFNWRQTARVLLFSIGLF"
     gene            complement(213..776)
                     /locus_tag="AC161X_01605"
     CDS             complement(213..776)
                     /locus_tag="AC161X_01605"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HIR4988276.1"
                     /translation="MGFTQAQLKAYEGQTVPDLVAPGIKLLFVGINPGLWTAATQTHF
                     CHPTNRFYPALRLAGLTDIEFDTDFGMSDSHRAQFLSRGIGITNLVARATARASEIGS
                     DELRMGGERLRATIGAVAPLVVAVAGVTAYRTAFGESGAMLGRQKGDLAGAETWVLPN
                     PSGLNAHETIATLSRWFAEVGKRARLL"
     gene            complement(784..1713)
                     /locus_tag="AC161X_01610"
     CDS             complement(784..1713)
                     /locus_tag="AC161X_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007517925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR4988277.1"
                     /translation="MSGPVQPLDGAVEQGPLAHIEIREGRSATVGATTVTRILPTKGR
                     RTVGPWCFVDLILPPDFEDPHPLEIGPHPHTNLATVTWLLEGSALHTDSLGTEQLIHP
                     GQLNLMTAGHGIAHAELGTTTGIHGVQMWLAQPEETRHGPSRFQHVAELPVVDFATAE
                     ATVMVGTLGAVESPAHVDTLMVGADVKARHGLLEVPLQTAFEYAVVPIDRSLKVGEDI
                     VEPGWLAMVPPGGDQIPIDVGPNGGRFLLLGGEPLGEPIEMWWNFVARSKDEITAAWR
                     AWQTHDEDWFGPVPSKLGRIDAPIPPWLPASEA"
     gene            complement(1739..2353)
                     /locus_tag="AC161X_01615"
     CDS             complement(1739..2353)
                     /locus_tag="AC161X_01615"
                     /inference="COORDINATES: protein motif:HMM:NF013286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR4988278.1"
                     /translation="MSEPTVISIAPDALDMIIQLRDQEPGDGEFALSIEVTGFRGPQF
                     TYELAFVALADKKDGWVVERHGELAVIFPEDDTPKLEGSSLDLTEEGLAMNNPNAPSS
                     PAMSGVHGDLTGPLVDQVQQVLNEQVNPAIAAHGGGAELVSVDGTIAYLRLYGGCQGC
                     GLAQVTLKQGIERTLLEAIPALTQVVDVTDHASGENPYIESQKK"
     gene            2368..>3498
                     /locus_tag="AC161X_01620"
     CDS             2368..>3498
                     /locus_tag="AC161X_01620"
                     /inference="COORDINATES: protein motif:HMM:NF014805.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HIR4988279.1"
                     /translation="MAATRKPMPTAVVVLPSTTYRASDFVAAAESLGVGLVVASEGPP
                     PIDMGDGYLQIDCTDPALAAETIADFGDRVGIDGVVAADDGGVIVAALTGQMLGLRSN
                     SPEAAAATRDKAAQRRALESAEVPQPRFMVVEPGDDADTAAAAISYPLVIKPIDRAAG
                     QGVMRVDQPEDLPPLLSRLRQIIGPSAPVLMESFMSGVEVAVEGLVSDGALTVLAVFD
                     KPDAGDGPFFPETIMVTPSRLPPGVIEECGRVAQGALDAIGITHGPVHIELMVDGAMV
                     RVIEVAARSIGGLCSKSLNFGLMSTSLETLILRNALGMDKPELHRESVASGVLMIPIP
                     THGAFKEIRNTEAVRRLPHVTGMDVTVPPGRQVEPPPEGDRYL"
BASE COUNT          638 a         1126 c         1140 g          594 t
ORIGIN      
        1 gaacaaaccg atcgaaaaca gcaggacacg ggcggtctgt ctccagttga acgccgacca
       61 aacggcgagg atgagccaca aagcgccgat cgtcagagtc cacggaaggg ggaccacgcc
      121 agtgaccccg gcaccgacga ccagcgccag aacgacgacg aagaagccgc cggcaaccag
      181 gccatagagg acctgtctgg gagtgggctc ggtcacaaca gcctcgcccg cttgccaacc
      241 tcggcaaacc agcgggaaag ggtggcgatg gtctcgtgtg cgttgagacc ggaaggattg
      301 ggtagcaccc aggtctcggc cccggcgagg tcccccttct gccggcccaa catcgccccc
      361 gattcaccga aagcagtccg gtacgcggtc accccggcaa cggcaaccac caagggggcc
      421 acggctccaa tggtcgccct cagccgctcc ccgcccatac gcagctcatc ggagccgatc
      481 tccgaggcgc gggcggtggc ccgggcaacc aggttggtga tccctatgcc tcgggacaga
      541 aactgcgctc ggtgggagtc gctcatcccg aaatcggtgt cgaactcgat atcggtgaga
      601 ccggcgaggc gcaaggccgg atagaagcga ttggttggat ggcagaaatg ggtttgggtc
      661 gccgccgtcc acagccccgg gttgatgccg acgaagagga gcttgatgcc gggggccacc
      721 aggtcgggga ccgtctgccc ctcgtacgcc ttgagctgtg cctgggtgaa tcccactggc
      781 ggttcaggcc tccgacgccg ggagccaggg tgggatcggc gcatcgattc gccccagctt
      841 cgaaggaacc gggccgaacc aatcctcgtc gtgcgtctgc caggctcgcc aggccgccgt
      901 gatctcgtcc ttcgagcgtg cgacaaagtt ccaccacatc tcaatcggct ctccgagcgg
      961 ctcaccaccc agaagtagga agcgcccgcc attaggaccg acatcgatcg ggatctggtc
     1021 accgcccggt gggaccatcg cgagccaccc cggctcgacg atgtcctcac ccaccttcag
     1081 gctgcgatcg atcggcacca cggcatattc gaaggccgtc tgcagtggga cctcgaggag
     1141 gccgtgtcgg gccttcacgt cggcaccgac catgagggtg tcgacgtggg ccggtgactc
     1201 gaccgctccc aatgtcccga ccatcaccgt tgcctcggca gtcgcaaagt cgaccaccgg
     1261 gagctccgcg acgtgttgga agcgggaggg tccgtgtcgg gtctcttcgg gttgcgccag
     1321 ccacatctgg accccatgaa tcccggtcgt ggtccccaac tcggcgtggg cgatcccatg
     1381 cccggcggtc atcaggttta gctgaccggg gtgaatcagt tgctcggtgc ccagggagtc
     1441 agtgtggaga gcggagcctt cgaggagcca ggtgaccgtg gccagattgg tgtgagggtg
     1501 ggggccgatc tccaagggat gcggatcttc gaagtcgggg ggaaggatca gatcaacgaa
     1561 gcaccagggc ccaacggtgc ggcgaccctt ggtggggagg atcctggtca cggtggtggc
     1621 accgacagtc gccgagcggc cctcccggat ctcgatgtga gccagcggcc cttgctcgac
     1681 agcaccgtcc agcggttgca ccggcccgct caagatgcct ccttgacgag ttgagccgct
     1741 acttcttctg gctctcgatg tatgggttct ctcccgacgc gtgatcggtc acgtccacga
     1801 cctgggtcaa ggcgggtatc gcctcgagca gggtgcgctc gatcccttgc ttgagagtca
     1861 cctgggccag cccgcagccc tgacagccgc catagagccg gagataagcg atcgtcccgt
     1921 ccaccgaaac cagctcggca cctcccccgt gggccgcgat tgccggattc acctgctcgt
     1981 tgagcacctg ctgcacctga tccacaagtg gaccggtgag gtcgccatgg acgccggaca
     2041 tcgcgggaga ggaaggcgcg ttcgggttgt tcatcgccaa gccctcctcg gtgaggtcga
     2101 gcgaagagcc ttcgagcttg ggggtgtcgt cctcggggaa gatcacagca agttccccgt
     2161 gacgttcgac aacccaccca tcttttttgt cggcgagggc gacaaacgcc agctcgtagg
     2221 tgaactgggg accacggaac ccggtgactt cgatcgagag ggcgaattcc ccgtctcccg
     2281 gctcctggtc gcgcaattgg atgatcatgt cgagcgcatc cggtgcgatg ctgatcactg
     2341 tgggttctga catctgcgag gatggtaatg gccgccacga gaaagcccat gccgaccgct
     2401 gttgtcgtcc ttcccagcac cacctaccgg gcatccgact tcgtggctgc cgccgaatcg
     2461 ttgggcgttg gcttggtggt ggcatcggag gggccgccgc ccatcgacat gggtgatggt
     2521 tacctccaga tcgactgcac cgaccctgct ctggctgcag agaccatcgc cgatttcggt
     2581 gaccgggttg ggatcgacgg agtggtcgcc gcggatgacg gcggtgtcat cgtcgccgcc
     2641 ctgaccgggc agatgctcgg cctccgctcc aattcaccag aggcagcagc cgccacaaga
     2701 gacaaggccg cccagcggcg cgccctggag tcagccgagg taccccaacc acgattcatg
     2761 gtcgtcgagc ccggtgacga cgccgacacg gctgccgccg ccatcagcta cccactggtg
     2821 atcaagccaa tcgaccgggc tgcaggtcag ggcgttatga gagtcgacca gcccgaggac
     2881 ctcccaccgc ttctgagtcg gttgcgccag atcatcggcc cctcggcccc ggttctcatg
     2941 gaatcgttca tgagtggagt ggaggtggct gtcgaaggtc tggtcagcga cggagctctc
     3001 acggtcctgg cggtgttcga caagccggat gccggagacg gacccttctt cccggagaca
     3061 atcatggtga ctccctcgcg acttccgcct ggggtgatcg aggagtgtgg gcgagtggct
     3121 cagggggcgc ttgacgccat cgggatcacc cacggaccgg tccatatcga gctgatggtg
     3181 gacggcgcca tggtgagggt catcgaggta gccgcccgct ctatcggcgg cctctgctcg
     3241 aagagcctca acttcgggct gatgagtacc tctctggaga ctttgatcct gcgcaacgca
     3301 ctggggatgg acaagccaga gctccacagg gagtcggtgg ccagcggcgt gctgatgatc
     3361 ccgatcccca cccacggagc attcaaggag atccgcaaca ccgaagcggt tcgtcgattg
     3421 ccacatgtga ccgggatgga cgtgacggtc ccccccggcc gccaggtcga gccaccaccc
     3481 gaaggcgacc gctatctg
//
LOCUS       DBNHXU010000059         3868 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4582661, whole genome shotgun sequence.
ACCESSION   DBNHXU010000059 DBNHXU010000000
VERSION     DBNHXU010000059.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3868)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3868)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3868
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4582661"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..196)
                     /locus_tag="AC161X_01625"
     CDS             complement(<1..196)
                     /locus_tag="AC161X_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HIR4988280.1"
                     /translation="MPNNTWARRSPAEQWQLDVTVGDGTETLWIYRRDPLVTRPWHEA
                     VLFSESGVPYLAPDIQLLFKS"
     gene            complement(215..436)
                     /locus_tag="AC161X_01630"
                     /pseudo
     CDS             complement(215..436)
                     /locus_tag="AC161X_01630"
                     /inference="COORDINATES: protein motif:HMM:NF022167.7"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
     gene            complement(400..1497)
                     /gene="tal"
                     /locus_tag="AC161X_01635"
     CDS             complement(400..1497)
                     /gene="tal"
                     /locus_tag="AC161X_01635"
                     /EC_number="2.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018333614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004801 - transaldolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaldolase"
                     /protein_id="HIR4988281.1"
                     /translation="MTSSRIHGLAKAGVSVWSDQISRAMLDSGELALRIDRDAVTGVT
                     SNPTIFAGAIVGSADYDQQLQDLKLAGVSPEEIAKNLMTADIQGGCDLLLGVFRDSGG
                     GDGFVSVEVSPTLAGDSQATVAEARDWVKQISRPNLLVKVPATPEGVPAIRALIGEGI
                     SVNVTLIFSLQRYDEVMEAYLAGLEDLRASGGDLSTIASVASFFVSRFDTEVDPRLEA
                     IGSDDALALRGVTAVANARVAYGLFLEKFASDRFRSLEADGARAQRPLWASTSTKNPD
                     YPDLIYVEGLVALDTVNTMPLPTIDAYQDHGSPSPAPFGTADIEQARQDLERLGEVGI
                     DYEEIVLILEEEGVDKFTKSWLELLERVEQA"
     gene            1557..1973
                     /locus_tag="AC161X_01640"
     CDS             1557..1973
                     /locus_tag="AC161X_01640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012234290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HIR4988282.1"
                     /translation="MPRRILIAKPGLDGHDRGAKVVARALRDAGFEVIYSGLHQTPAQ
                     IAETAIQEDVDAVGLSSLSGAHRTLFPEVVTELASRQGEDIIVFGGGVIPEADIATLV
                     TAGLATVFTPGTPLDEITEWVDAHVPSRSNQKKETP"
     gene            1970..3193
                     /gene="sucC"
                     /locus_tag="AC161X_01645"
     CDS             1970..3193
                     /gene="sucC"
                     /locus_tag="AC161X_01645"
                     /EC_number="6.2.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013177752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-forming succinate--CoA ligase subunit beta"
                     /protein_id="HIR4988283.1"
                     /translation="MKIHEYQAKQLMAARGIPVPQGRLATSVEEAVAAVRPLIEESGN
                     PVVVLKSQIHAGGRGKGTFLEHPEVRGVNVITDAIEDGPEAAEAKVRDLASKMLGSTL
                     VTIQTGSEGKVVSRLYLEQGIDIDRELYLAVLLDRSVGRNIVMASTEGGMDIEKVAQE
                     TPEKILREEVDPAIGFADFQANELAHSLGLRGEAASNFGPFVKALVAAAGELDTDLIE
                     INPLVVSTDGRIVALDGKMGFDDNALFRHPEVVAMRDESEEDPAELAARDAGLNYIKL
                     EGTIGCLVNEAGLAMATMDTIKHVGGEPANFLDVGGGADADQVATGFQIITSDPNVKG
                     IFVNIFGGIMRCDVIATGIVEAVERVGLKVPLVVRLEGTNVELGRQILRDSGLDLVAA
                     DSLRDGAEKILELTR"
     gene            3190..>3868
                     /locus_tag="AC161X_01650"
     CDS             3190..>3868
                     /locus_tag="AC161X_01650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012810730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinate--CoA ligase subunit alpha"
                     /protein_id="HIR4988284.1"
                     /translation="MSILVGKDTKVLVQGLGRTGQFHTDKAIAYGTRMVAGVHPSRAD
                     TAMHFEGETDHSGVPDRDDAYSVDLPVYATVKEAVAATGANASAIYVPPPFAADAIME
                     AAAAGLDLVVAITEGIPVADMIRAKRYLSDKKTRLIGPNCPGVITPGECKIGIMPGYI
                     HQRGRVGVISRSGTLTYEAVHQLTLLGLGQTTAVGIGGDPVNGTNFVDVLQMFENDAE
                     TEGVVMIG"
BASE COUNT          754 a         1191 c         1246 g          677 t
ORIGIN      
        1 tggacttgaa caggagctgg atatcgggcg cgaggtaggg gactccggat tccgaaaaca
       61 ggacggcttc atgccagggc cgggtcacca gcggatcgcg acggtagatc caaagagtct
      121 ctgtcccgtc accgacggtc acgtctagct gccactgctc agcgggcgaa cggcgggccc
      181 aggtgttgtt aggcatgact gcgaagtccc aaccggagag ccaggcgaac agctctgctg
      241 actggtcccg gcacacccca acatcgagat cgtcgtggtc acgccaggaa cgcccggttt
      301 gtagctcgag ggctctacca cccgagatcc accagcggaa cggcgctcct tgaaagacat
      361 caactgcctc ttcgacagag agcggcgacc atgctccgct caggcctgct ccacccgctc
      421 cagcagttcc aaccagctct tggtgaactt gtcgacgcct tcctcttcga ggatcagaac
      481 tatttcctcg tagtcgatgc cgacctcccc gagtcgctcc agatcctgtc gtgcctgctc
      541 gatgtcggct gtgccgaacg gagccggcga agggctcccg tgatcctgat aggcgtcgat
      601 ggtgggcagc ggcatcgtgt tgacggtgtc gagagccacc agtccctcca cgtagatcaa
      661 atcggggtag tcggggttct tggtcgaggt ggaggcccac agcggtcgct gagcccttgc
      721 gccgtcggct tccagcgacc ggaaacgatc cgacgcgaac ttctcgagga acaagccgta
      781 ggcgactcgg gcattggcga ctgcggttac gccacgcagg gcgagggcat cgtcggaccc
      841 gatggcctcg agacgggggt cgacctcggt gtcgaatcga gacacaaaga acgatgcgac
      901 cgacgcgatg gtggacaggt cgccgccaga cgcccgaagg tcctccaggc ccgccagata
      961 ggcctccatc acctcgtcgt agcgctgcaa cgagaagatc agcgtgacgt tcacggaaat
     1021 cccctctccg atcagagccc tgattgcggg aaccccctcc ggtgtggccg gcaccttgac
     1081 caggaggttg gggcggctga tctgtttcac ccaatcccgg gcctcggcca ccgtggcctg
     1141 actgtcacca gcaagggtgg gagacacttc gaccgagacg aaaccgtccc caccgccgct
     1201 gtcgcggaac accccgagca gcagatcaca gccaccttgg atgtcggcag tcatcaggtt
     1261 cttggcgatc tcctcggggc tgactcctgc caacttgaga tcctgaagtt gctggtcgta
     1321 gtcggccgag ccgacgatgg cgccggcgaa gatcgtgggg ttggaagtca ccccagtcac
     1381 cgcatcacga tcgatgcgca gggccagctc acccgagtcg agcatggcgc gggagatctg
     1441 atccgaccag accgagactc ccgctttcgc caacccgtgg attcgtgaag aggtcactca
     1501 cgaagggtag ggcgaagcgg cgcatgttca gttggcaact agccttgccg ccccgcatgc
     1561 ctcgccgcat tctcatcgcc aagcccgggc tcgatggcca cgatcggggc gcaaaggtcg
     1621 ttgcccgagc gttgcgcgac gccggcttcg aggtcatcta ctccggcctt catcagactc
     1681 ccgcccagat cgcggagacc gcgatccagg aggatgtcga cgcggtcggt ctttcctcat
     1741 tgtcgggtgc ccatcgaacc ctgttccccg aggtcgtcac cgagttggca tcccgccagg
     1801 gtgaagacat catcgtgttt ggaggcgggg tgatccccga ggctgacatc gccaccctgg
     1861 tcaccgccgg gctggcgact gtcttcacac cggggacacc gttggatgag atcaccgagt
     1921 gggtcgatgc tcacgttccg tcccgttcaa atcagaagaa agagacacct tgaagattca
     1981 cgagtaccag gccaagcaac tcatggcggc ccgcggcatc ccggtgccgc agggtcggct
     2041 ggcgacctcc gtggaggagg ctgttgccgc ggtgaggcct ttgatcgagg agtcggggaa
     2101 cccggtcgtc gtcctcaagt cacagattca tgccggcggc cgggggaagg gaacctttct
     2161 cgagcacccc gaagtgcggg gagtcaacgt catcaccgac gccatcgagg atggtcccga
     2221 ggctgctgaa gcgaaggtcc gtgacctggc atcgaagatg ctgggatcga cgttggtcac
     2281 cattcagacc ggcagtgagg gcaaggtggt cagccgtctc tatctcgaac agggcatcga
     2341 catcgaccgt gagctctacc tggcggtcct gctcgaccgc agcgtcgggc gcaacatcgt
     2401 catggcctca accgaaggtg gcatggacat cgagaaagtt gcccaggaga cgcccgagaa
     2461 gattctccgc gaagaggtcg acccagcgat tgggttcgca gattttcagg ccaatgaatt
     2521 ggcgcactcc cttgggctcc gcggagaggc ggcctccaac ttcgggccct tcgtcaaggc
     2581 tctggtggcg gcagccgggg agctggacac agacctcatc gagatcaacc cacttgtggt
     2641 atccaccgac gggaggatcg tcgccctgga tggaaagatg ggattcgacg acaacgccct
     2701 cttccgtcat ccggaggtgg tggcgatgcg cgatgagagc gaagaggatc cagccgagct
     2761 ggcggctcgc gatgccggcc tcaactacat caagctcgag ggcaccatcg gctgcctggt
     2821 caacgaggcc ggtctggcga tggcgaccat ggataccatc aagcatgtcg ggggggagcc
     2881 ggcaaacttc ctcgacgtcg ggggtggcgc ggatgccgac caagtagcga ccggcttcca
     2941 gatcatcacc agcgacccca acgtgaaggg gattttcgtc aacatcttcg gcggaatcat
     3001 gcgatgtgat gtcatcgcca ccgggattgt cgaggccgtg gaacgtgtcg gtctcaaggt
     3061 gcccctggtg gtgaggctcg aagggaccaa tgtcgaactg ggcaggcaga tcctgaggga
     3121 ctcgggcctc gatctggttg cagctgacag tcttagagac ggcgccgaga agatcctgga
     3181 gctgacccga tgagcatcct ggtcggcaag gacaccaaag tcctggtgca gggtctcggc
     3241 aggaccgggc aattccacac ggacaaggcg atcgcctatg ggacccggat ggtggccggc
     3301 gtccatccgt cgcgggcgga cacggccatg catttcgaag gggagaccga tcactctggc
     3361 gtcccggacc gtgatgacgc ctattcggtc gatctacctg tgtatgccac ggtgaaggag
     3421 gcagtggcgg caaccggagc caacgcctcg gcaatctacg tcccgccgcc cttcgctgcc
     3481 gacgccatca tggaggcggc agccgccggt ctcgatctgg tcgttgccat caccgagggg
     3541 atcccggtgg ccgacatgat ccgcgccaag cggtacctat ccgacaagaa gacccgtctc
     3601 atcggcccca attgccccgg tgtgatcacc ccaggcgagt gcaagatcgg gatcatgccc
     3661 ggttacatcc atcaaagagg ccgggtgggg gtgatctcgc gatcggggac tctgacctat
     3721 gaggcggtgc accaactcac gctgctcggg ctgggccaga ccacagcggt ggggatcggt
     3781 ggcgacccgg tgaatgggac caacttcgtc gatgtcctcc agatgttcga gaacgacgcg
     3841 gagactgaag gtgtggtcat gatcggtg
//
LOCUS       DBNHXU010000060         2794 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2202440, whole genome shotgun sequence.
ACCESSION   DBNHXU010000060 DBNHXU010000000
VERSION     DBNHXU010000060.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2794)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2794
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2202440"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /gene="purN"
                     /locus_tag="AC161X_01655"
     CDS             <1..323
                     /gene="purN"
                     /locus_tag="AC161X_01655"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011196971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006974 - DNA damage response [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HIR4988285.1"
                     /translation="PEAIGRFPNRILNIHPSLLPAFPGSNAVAQALAHGVKVTGVTVH
                     LVDEEVDHGPIIAQRAVPVLPGDDVTVLHARIQVQEHDLYPRVVRAFAEGDVSIVGRE
                     VIWR"
     gene            320..1819
                     /gene="purH"
                     /locus_tag="AC161X_01660"
     CDS             320..1819
                     /gene="purH"
                     /locus_tag="AC161X_01660"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013130918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HIR4988286.1"
                     /translation="MSVVHVTRALISVYEKAGLIPFAQRLAAAGVAIVSSGGTAQALS
                     DAGVAVTPVEDLTGAPEMFGGRVKTLHPMVHGGILARLDNDSDVADMEANGIEPFQLV
                     VVNLYPFRETVASGAGEEEIIEKIDIGGPAMVRAAAKNYRHVGIVSSPDQYEEVADAV
                     ERGGLDADLRRRLAADAFFRTASYDAAIVGWIGDDLVIPLRRVSELRYGENPHQEAAL
                     YVEDGSRPWWQQAMQLQGKEMSFNNYADAEAAWRLANSVPGMVVIVKHTNPCGAAHGG
                     EVADAFERAWACDPLSAFGGVIAVNGVVDEKTASSIAEKFVEVLVCAGVSDEALDVLG
                     RKSAVRVLVAAAPGVSDPGMSRLEQGFLVQTRDGQSTDAWEVVSERLPTDAEREDLEF
                     GWIVAMNTKSNAIVIVKDGAAIGVGAGDQSRVGAAERALARAGDRAGGGAAASDAFFP
                     FRDGLDVLADAGVTAVIEPGGSRNDQEVIDAANQHGIALVFASNRHFRH"
     gene            1822..2667
                     /gene="folD"
                     /locus_tag="AC161X_01665"
     CDS             1822..2667
                     /gene="folD"
                     /locus_tag="AC161X_01665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014909936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HIR4988287.1"
                     /translation="MSALILDGKAVASAVKSEVATAVATLDYAPGLATVLVGDDPASH
                     TYVRGKRSDAAEVGIVSMHHELPTDVSQGDLEDLIEQLNADEAVDGILVQLPLPGDLD
                     SERIVERIVPAKDVDGLHPHNLGLLVLDRPGLRPCTPSGIMRILAHYGLPVSGARAVV
                     IGRSFLVGRPLALMLASRGVDATVTIAHSRTKDLAAVAREADILVAAVGSPGLVTADF
                     VKPGATVIDVGINRTDGGMVGDVDFESVLGLAGAVTPVPGGVGPMTRAMLLVNTLTAA
                     RARRA"
BASE COUNT          488 a          819 c          970 g          517 t
ORIGIN      
        1 ccccggaggc aatcggccgg tttccgaatc ggatcctcaa cattcacccg tccttgctgc
       61 ctgcgttccc tggctcaaat gcggtggccc aggctctggc tcatggggtg aaggtgaccg
      121 gggtgacagt ccatctggtg gacgaagagg tggatcacgg gccgatcatc gcccagcgcg
      181 ccgtcccggt cctgcccggt gacgacgtga ctgtgctcca cgctcggatc caggtgcaag
      241 agcacgatct ctatccacgg gtggttcgag ctttcgccga aggagatgtc agcatcgtgg
      301 ggagggaagt gatttggcga tgagtgtcgt ccacgtgacc agggcgctga tcagtgtcta
      361 tgagaaagcg ggcctgattc cgtttgccca gcggcttgcc gccgccggtg tcgccatcgt
      421 ctcctctgga gggacggccc aggcattgag cgatgccggg gtggcggtga cgccggtgga
      481 ggacctgacc ggagcccccg agatgttcgg gggcagggtg aagacccttc accccatggt
      541 ccatggcggg atcctggccc gactcgacaa cgattccgac gtcgccgata tggaggccaa
      601 cggcatcgag cccttccagc tagtcgttgt caacctctac ccgttccggg agaccgtcgc
      661 ctctggcgcc ggagaggaag agatcatcga gaagatcgac atcggtggac ccgcgatggt
      721 ccgggcggcc gcgaagaact accggcatgt cgggatcgtg agctcgccgg accaatatga
      781 ggaagtggcc gatgccgtag agcgcggtgg cctggacgcc gaccttcgga gacgactggc
      841 ggccgacgcc tttttccgga ccgcctccta cgacgcggcg atagtcgggt ggataggtga
      901 cgatctggtg attcccctgc gccgcgtttc ggagctccgt tatggcgaga acccacacca
      961 ggaggctgct ctgtacgttg aggacggctc ccggccctgg tggcaacagg ccatgcagct
     1021 ccagggcaag gaaatgtcgt tcaacaacta tgccgatgcc gaggcggcct ggagacttgc
     1081 caactcggtt ccgggcatgg ttgtcatcgt gaagcacacc aacccttgtg gtgcggccca
     1141 tggtggggag gtcgccgatg cattcgagcg ggcctgggct tgtgacccgc tgtcggcgtt
     1201 cggtggggtg atcgcagtga acggcgttgt cgatgaaaag acggcaagtt cgattgcgga
     1261 gaagttcgtc gaagttctcg tgtgcgccgg cgtcagcgac gaggcgctcg atgtgctggg
     1321 ccggaagtcg gccgtccggg tattggtggc ggccgctcct ggggtgagcg atccaggtat
     1381 gagccgtctc gagcagggat tccttgttca aacgagggat ggccaatcga ctgatgcctg
     1441 ggaggtcgtg tccgaaagac tcccgaccga cgcggaaaga gaggatctgg aattcggctg
     1501 gatcgtggcg atgaacacca agtcgaacgc catcgtgatc gtgaaggatg gggccgcgat
     1561 cggggtaggt gcaggagacc aatcgcgcgt gggggcggca gagcgggctt tggcgcgggc
     1621 cggggatcgg gctggtggtg gtgcagccgc gtcggacgcc ttcttccctt tccgtgacgg
     1681 gctggatgtc ctggccgatg ccggggtcac agccgtgatc gagccgggcg gctcgcgaaa
     1741 tgatcaggaa gtgatcgatg ccgcgaatca gcacggaatc gccctggtgt tcgccagcaa
     1801 ccgccatttt cgccactgag catgagtgct ttgatcctgg acgggaaggc agttgcgtcg
     1861 gcggtgaagt cagaggtcgc caccgcggtc gccaccctcg attacgcccc cggtctggcc
     1921 acggttctcg tcggggacga ccctgcctcg cacacgtatg tccggggcaa gcgcagcgat
     1981 gcagctgaag tgggcatcgt ctcaatgcac cacgagttgc cgaccgatgt ttcccagggc
     2041 gatctcgagg acctgatcga gcagctcaac gccgatgagg cagtcgacgg gatactcgtc
     2101 caactgccat tgccgggcga cctcgactcg gagcgaatcg tggagcgaat cgtgccggcc
     2161 aaggatgtcg acgggctcca tccccacaac ctggggcttt tggtactcga tcgccctggt
     2221 ctgcggccgt gtactccgag cgggatcatg aggattctgg cccactatgg gttgcccgtc
     2281 tcaggcgcga gggccgtggt catcgggaga tcgttccttg taggccggcc tctggccctc
     2341 atgctcgcca gcagaggggt ggatgccacg gtgaccatcg cccactcccg caccaaggat
     2401 ctggcggcgg tcgcccgcga ggccgacatc ctggttgcgg cggtcggctc acccggattg
     2461 gttactgcgg acttcgtcaa gccgggggca acagtgatcg acgtcggcat caaccggacg
     2521 gatggcggaa tggttggcga cgtcgacttc gagtcagttc tgggtctagc tggggcggta
     2581 accccggtgc ccggcggagt gggcccgatg accagggcaa tgcttctcgt caacaccctg
     2641 accgccgctc gggcccgacg cgcctgaccc ggggtctggt ctgctgcgca cacgggtatg
     2701 aggcttgagt ggatcacgct cgaggccgat aaacaggtag tcgattcact gccccgggaa
     2761 tcacccaagg aacaatgaga gctctgcaca agac
//
LOCUS       DBNHXU010000061         7114 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4979984, whole genome shotgun sequence.
ACCESSION   DBNHXU010000061 DBNHXU010000000
VERSION     DBNHXU010000061.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7114)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7114)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7114
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4979984"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="AC161X_01670"
     CDS             complement(<1..256)
                     /locus_tag="AC161X_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988288.1"
                     /translation="MSPQSASVPTIPARLTIAEFAAATERSVAEVQAILEDRSEPYGE
                     EDMIDPDVSVSLAKALGIQINIEPRDLALETLYELETRGDD"
     gene            complement(253..810)
                     /gene="efp"
                     /locus_tag="AC161X_01675"
     CDS             complement(253..810)
                     /gene="efp"
                     /locus_tag="AC161X_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015050733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HIR4988289.1"
                     /translation="MISTNDVRPGMALQLNDGLFTIVEYQHVKPGKGKAFVRMKIKNL
                     ETGQVLDRTFRADEHVDQAIIDRRDHQYLYSDDLGFHFMNLEDFGQLALSTDDVGEAA
                     GYLIEGMTVSLPLYRGTPIGVDLPASVELEVTYAEPAVKGDTRTGATKPVTLQTGIQI
                     QVPLFVEQGDRVKVDTRSGEYLTRA"
     gene            complement(856..1881)
                     /gene="aroB"
                     /locus_tag="AC161X_01680"
     CDS             complement(856..1881)
                     /gene="aroB"
                     /locus_tag="AC161X_01680"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HIR4988290.1"
                     /translation="MGTLTVAGRTEVLVGEGVPDDVLPVREGRQRVAILTQPGATRYA
                     LDISRVLKEEGLGVEVIGLPDREEAKTLTVAGTVYETLARFGLGRQDTVIGVGGGSVS
                     DLAGFVAGTWLRGVEVVHVPTTLLAAVDASIGGKTGVNLAGKNLVGLFWHPTRVVVDV
                     RILKGLPSSLLREGMVEALKTGLVGDPDLFATIEKHGVEADLDDVVVRSATVKARMVG
                     EDEREFGIRAVLNLGHTIGHAIEFASPLSHGESVAVGLVAAARISEIRLGFEDASRIS
                     ESIQRLDLPITVTGLDRARVIDLLRHDKKRDAQGIRMVLLRAVGAPVVQHVSSDDIEV
                     GLESVGL"
     gene            complement(1869..2372)
                     /locus_tag="AC161X_01685"
     CDS             complement(1869..2372)
                     /locus_tag="AC161X_01685"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: protein motif:HMM:NF013375.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004765 - shikimate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HIR4988291.1"
                     /translation="MTLWLAGMMGSGKTSSGAVAAASLGVPFHDTDVEVTAGLDCTVS
                     ELWERVGEAIFREMESVTVKRLAGLDAIVATGGGAVLQAVNRDVMTASGPIVWLQASP
                     EEILERVGATIGRPLLDNSSDPLEELRVLSKQRADIYAEVADHVIETSGKSVAEVAEE
                     IESLWEL"
     gene            complement(2369..3526)
                     /gene="aroC"
                     /locus_tag="AC161X_01690"
     CDS             complement(2369..3526)
                     /gene="aroC"
                     /locus_tag="AC161X_01690"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011291537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HIR4988292.1"
                     /translation="MFRFMTAGESHGPGLVTVVEGLPRGLSVSAEGIADELARRRLGF
                     GRGKRMAIERDSLEILGGVRHGFTLGSPVAVLVRNTEWPRWAEEMSPEPAPVKKPVTR
                     PRPGHADLAGMLKYDTEDARDILERASARETAARTVAGHLAKILLRTVGVEILSHVVS
                     IGHVESEGPTPLPADLPAIDDSPVRCFHEASAQAMIELIEDAKERRDTLGGVFEVLGY
                     GVPAGIGSHVHYDRRLDGMLAAGLMSIPAIKGVEIGDGFEVALLPGSKSHDEIVSGEV
                     GLERPTNRAGGLEGGMSNGEPIRVRAAMKPISTLMRPLRTVDMSTGEPAEAVRERSDV
                     CAVPAAGVVGEQMVAITLASEFQRKFGGDTVDEFVAAHTRYVDGLAERLKR"
     gene            complement(3537..4322)
                     /locus_tag="AC161X_01695"
     CDS             complement(3537..4322)
                     /locus_tag="AC161X_01695"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: protein motif:HMM:NF013632.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HIR4988293.1"
                     /translation="MIYVLAGAFGFLGAFAAHDLASQTLRDVPLRPYTGTCPRCGNQR
                     GWFKWTCPSCSRRIGRELILALVGALIAIGFARTIGATWSLVPYLGFLVLTLALGITD
                     IDAFRIVDRLNLRGTAILAALLAIAAVLDGSIPELGRAALGALAYFGGSNVMFILAGG
                     KGFGYGDVKLSVQLGLFTAYLSWGTLGWAVLITALLGGLASMILISVGAVARSRAKRK
                     APEGETVSLRDVMKTELPYGPAMIAGAWAAIILVGLGAIPIPT"
     gene            complement(4366..5559)
                     /locus_tag="AC161X_01700"
     CDS             complement(4366..5559)
                     /locus_tag="AC161X_01700"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR4988294.1"
                     /translation="MALSGNLGFVPLDEVLRLLTRSDQQGAIDVWGQDVRGRIFISRK
                     GIGLATISDDKELHRHLVNSGYVDEAYISSIESGTTTLAPLADKEGDIVDLLREMTVE
                     SVYQMSRRGSSFEVTESVSSPFASPRAFELEAILEESRRRAGEWSEVEQIISDLDGVI
                     KMNRDLGEQDSVKISRDAWRLLCEMGSGASVRTLAERLGTTDFWTARVAAGLSEQQLL
                     VLAESVAEEEVVAPVAVEQTSYYEQPAAAEVAPAVDEDKDTDESWWVEPTAEENEAAA
                     AAAADVAESAQSEPEAHKDSRFGQFRQKVETADEPANEAAEAASGTEAPFEPDSSVFE
                     PQSEAVPSLETPSDLDAVEEDTEAFLEKVFSQLDTNGQPEEEGHGLLRRRRLGSVLKE
                     MTEED"
     gene            complement(5620..6483)
                     /locus_tag="AC161X_01705"
     CDS             complement(5620..6483)
                     /locus_tag="AC161X_01705"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase family protein"
                     /protein_id="HIR4988295.1"
                     /translation="MSTTPMSSRLGPTESFPEHRLGSQTAMRADLRFALLGDPVDHSR
                     SPAIHRAALTELGLSGTYEARRSGQAGLEAAVDELRDGDLDGINITMPLKALAAASAD
                     RLTELADRSGSVNTLRFQDDLVEGHSTDAAATATVLAQSRFDRSAPILVLGAGGAAAA
                     VLVALRGREVHLAARRGDLAREVAAKAGYEVRLVPFGTAVAGAIVINATPLGMKGEPL
                     PDGVVDGASGLIDLAYGPVEPPATAEARRIGLPLVDGLEFLALQAGESFTWWTGMAAP
                     LEVMLKAAKNG"
     gene            complement(6431..6982)
                     /gene="mltG"
                     /locus_tag="AC161X_01710"
     CDS             complement(6431..6982)
                     /gene="mltG"
                     /locus_tag="AC161X_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HIR4988296.1"
                     /translation="MALADWEGLLFPDTYEFTKSAEPVAILQRLASTMEQRVDSIDWS
                     VVEAAGFTPYEGLIIASLVETEVLLDEERPIVSSVIHNRLAIDMKLDIDATVLYALDT
                     RDIAEFNNEVDSPYNTYVVAGLPPTPIASPGRASLEAAAAPDTTDYLYYVLSDLEGHH
                     AFAATLDEHNANVEQARADGVLP"
BASE COUNT         1339 a         2329 c         2164 g         1282 t
ORIGIN      
        1 cgtcgtcacc ccgagtctcc aactcataca gagtctccag agcaaggtcc cgcggctcga
       61 tgttgatctg gatgccgagc gcttttgcca gcgacactga gacatcgggg tcgatcatgt
      121 cctcttcccc gtatggctca gatcggtcct cgagtatcgc ctgaacctct gcaaccgagc
      181 gctctgtggc cgcggcgaac tcggcaatag tgagacgggc cgggattgtc ggcactgatg
      241 ccgattgggg gctcatgctc tggtgaggta ctccccgctc cgagtgtcga ccttcacccg
      301 atcgccctgt tcgacgaaaa gcggcacctg aatctgaatc ccggtctgaa gcgtcactgg
      361 cttggtggcg ccggtgcggg tatcgccttt gacggctggc tcggcatagg tgacctcgag
      421 ttccaccgac gccggcaggt ccaccccgat cggggtgccc cgatagaggg gaaggctgac
      481 tgtcatccct tcgatcagat accccgcggc ctcaccgacg tcatcggtgg acaacgccag
      541 ttgtccgaag tcctcgaggt tcatgaagtg gaagcccaaa tcgtccgaat acaggtattg
      601 gtgatcccgc cggtcgatga tcgcctggtc gacgtgctcg tcggcccgaa acgtgcgatc
      661 gagaacctgg ccggtttcga ggttcttgat cttcatccgg acgaaggcct tgcctttgcc
      721 cggcttcaca tgctgatact cgacaatggt gaacagacca tcgttgagct gcaatgccat
      781 gccgggtcgt acgtcgttgg tgctgatcat tcaagaggtc ctggttgagg cgattcgggc
      841 acggacggta cccgctcaga gaccaacgga ctccagcccg acctcgatgt cgtcactgct
      901 cacatgttgc accacgggag cgcccacagc ccggagcaga accatgcgta ttccctgagc
      961 gtcacgcttc ttgtcatgac ggagcaggtc gatcaccctc gcccgatcca ggccggtgac
     1021 ggtgatcggc aggtccagcc gctggatcga ctccgagatc cggctcgcat cctcgaagcc
     1081 gaggcgtatc tccgagatcc gggccgcggc aacgagaccg actgccacgc tctcgccgtg
     1141 agagaggggc gaggcgaact cgatggcatg gccgatcgta tggcccaggt tgagtaccgc
     1201 tcggatcccg aactcacgct cgtcttcgcc gaccatcctc gccttcacgg tggccgaccg
     1261 gaccacgaca tcgtccagat cggcctcaac cccatgcttt tcgatcgtgg cgaacaggtc
     1321 gggatcgccc acgaggcccg tcttcaacgc ttcgaccatg ccctctcgca acagcgatga
     1381 gggcaggccc ttgagaatcc gaacgtcgac aaccacccgg gtcggatgcc agaacagacc
     1441 cacaaggttc ttgcccgcca gattgactcc ggtcttgcct ccgatggagg cgtccaccgc
     1501 ggcgagcagt gtggtcggca cgtggacgac ttcgacaccc cggagccagg tgccggcgac
     1561 gaagcccgca aggtccgaca ccgacccacc tccgactccg atcacggtgt cctggcggcc
     1621 aagaccgaag cgggccagag tctcataaac ggtcccggcg acggtgagtg tcttcgcttc
     1681 ttcacgatcc ggcagcccga tgacctcgac cccgagtcct tcctctttga gaacccgcga
     1741 gatgtcgagg gcatatcggg ttgctcccgg ttgggtgagg atggcgaccc gctgtcttcc
     1801 ctcccgtacg ggaaggacgt catcgggaac accctcccca acgagcacct cggttcgccc
     1861 ggcgacggtc agagttccca aagcgactcg atctcttctg cgacctcggc gacactcttg
     1921 cccgaggttt cgatcacgtg atcggcaacc tcggcgtaga tgtcggcgcg ttgcttggaa
     1981 agcactcgaa gctcttccaa ggggtcggag ctgttgtcga gaagcgggcg cccaatggtc
     2041 gccccgactc gctccaggat ctcttcggga gaagcctgca gccagacgat cggcccggag
     2101 gcggtcatca catcacgatt cactgcctgg agcacggcgc cacctccggt cgcaacgatg
     2161 gcgtccaacc cggcgagtcg tttgacagtg accgactcca tctcacggaa tatcgcttcc
     2221 ccaacccgct cccagagctc ggaaaccgtg caatccaagc cggctgtgac ctcgacgtcg
     2281 gtgtcatgga agggaacccc caaactggca gcagcgacgg caccgcttga ggtcttgcct
     2341 gaccccatca tcccggcgag ccatagcgtc atcgcttgag acgctcggcc aacccatcca
     2401 cgtatcgagt gtgggcggcc acgaactcat cgaccgtgtc cccgccgaac ttgcgctgga
     2461 actccgaggc gagggtgatg gcaaccatct gctcgccgac caccccagct gcgggaactg
     2521 cacacacatc ggaccgctcc cgcaccgctt cggccggctc accggtgctc atgtcgacgg
     2581 tccgaagcgg gcgcatcaga gtggatatcg gtttcatggc cgccctgacc cgaatcggct
     2641 cgccattgct catcccgccc tcgaggccgc cggcgcgatt ggtgggccgc tccagcccga
     2701 cctctcccga aactatctcg tcatgcgact tcgatcccgg gaggagggca acctcgaatc
     2761 cgtcgccaat ttcgacgcct ttgattgcgg ggatcgacat caggccggcg gcgagcatgc
     2821 catcgagacg gcggtcatag tggacgtgag agccaattcc cgccgggacc ccatatccaa
     2881 gaacctcgaa aacgccgccg agagtgtcac gccgctcttt ggcgtcctcg atcaattcga
     2941 tcatcgcctg tgccgaggcc tcgtgaaaac aacggactgg actgtcatcg attgccggca
     3001 gatcggcggg aagaggggtt ggcccctcgc tctcgacgtg gccgatcgaa accacgtggc
     3061 tgaggatctc gacgccgacc gttcgaagca aaatcttcgc caggtgcccg gcaacagttc
     3121 gagccgcggt ctctctggct gacgccctct cgaggatgtc gcgggcgtct tcggtgtcgt
     3181 acttgagcat cccggccagg tcggcatggc caggccttgg cctggtgacc ggcttcttga
     3241 cgggcgccgg ctcggggctc atctcctccg cccagcgagg ccactcggtg ttcctgacca
     3301 ggaccgcaac aggcgaccca agagtgaaac cgtgtcggac ccctcccagg atctcgagag
     3361 agtcacgctc gatcgccatg cgcttgcctc tcccgaatcc gagacggcga cgcgccaatt
     3421 cgtctgcgat tccctcggcc gacacagaga ggcctcgggg caggccctca accactgtca
     3481 ccagcccggg gccatgtgac tcaccggccg tcatgaagcg aaacatggga gggatgctac
     3541 gtcgggatcg ggatcgctcc gagcccaacg aggatgatcg ccgcccaggc gccggcgatc
     3601 atcgccggcc cgtagggcag ttcggtcttc atgacatcac gaagtgacac ggtttcgccc
     3661 tcgggcgcct tccttttggc gcggctcctc gccactgcgc cgacggaaat caagatcatc
     3721 gacgccaagc cgccaagaag cgcggtgatc aggacagccc aaccgagcgt tccccaggag
     3781 aggtatgcgg tgaacaggcc aagttgcacc gagagcttga cgtctccata gccgaacccc
     3841 ttgccaccgg cgaggatgaa catcacattg ctgcccccga aataggccag ggcaccgaga
     3901 gctgcccggc ccaactcagg gatggagcca tcgaggactg ccgcgattgc aagcagcgcg
     3961 gccaggatcg ccgtgccccg cagattgagt cgatcgacga tgcggaaggc gtcgatgtcg
     4021 gtgattccca gggcaagggt caggaccaag aatccgagat agggaacgag agaccaggtg
     4081 gccccgatag tgcgggcaaa cccgatcgca atcagagctc caaccaatgc caggatcagc
     4141 tcgcgaccga ttctccgcga gcacgaggga caggtccact tgaaccaacc acgctgattg
     4201 ccacaacgag ggcaggtccc cgtgtacggg cgcagcggga cgtcgcgcag ggtttgagac
     4261 gcgagatcgt gggcggcaaa cgcccctaga aagccaaaag ctccggcgag gacatatatc
     4321 accgccggag caaattagcg ttgggaaagg tcgagagcct ggctctcagt cttcctcagt
     4381 catttccttg agcacggagc cgaggcgacg gcgccgaagc aggccatggc cctcttcctc
     4441 tggctggcca ttggtatcga gctgcgagaa caccttttcc aggaaggctt cggtgtcttc
     4501 ctcgaccgca tccaggtcgg agggtgtctc cagcgagggc acggcctcgg actggggctc
     4561 gaaaactgac gaatcgggct cgaacggtgc ctcagttccc gacgctgctt ccgctgcctc
     4621 attggcgggc tcgtccgcag tctcaacctt ctggcggaac tggccgaatc ggctgtcctt
     4681 atgggcttct ggctccgact gcgcggattc ggcgacatcg gcagcagccg cagcagccgc
     4741 ctcgttctcc tccgccgtgg gctcgaccca ccacgattcg tcggtgtcct tgtcctcatc
     4801 gactgcagga gcaacttcgg cagcggcggg ctgctcgtag tacgaggttt gttcgactgc
     4861 gacaggcgcc acaacctctt cctcggcgac actttcggcg aggacgagca gttgctgctc
     4921 ggaaagaccg gctgccaccc gggcagtcca gaagtcggtg gtgccgagac gctcggcaag
     4981 tgtccgcacc gaagcgcccg atcccatctc gcagagaagg cgccaggcat cgcggctgat
     5041 cttgaccgag tcctgctcgc ccagatcgcg gttcatcttg atgacgccat ccaggtcgga
     5101 gatgatctgc tcgacctcgg accattcccc ggcgcgccgt ctcgactctt cgagaatcgc
     5161 ctcgagctca aaggccctcg gactggcgaa aggagagcta accgactcgg tcacctcgaa
     5221 ggacgacccg cggcgggaca tctgatagac gctttcgacg gtcatctcac gaagaagatc
     5281 gacgatgtcg ccctctttgt cggccagagg agccagcgtg gtggtgccgg actcgatcga
     5341 gctgatatac gcctcgtcga cgtagcccga gttgaccagg tgacgatgaa gttccttgtc
     5401 gtcggagatg gttgcgaggc cgatcccctt ccggctgatg aagatgcgtc ccctgacgtc
     5461 ctgaccccag acgtcgatgg caccctgctg gtctgagcgg gtcagaagcc gaagcacctc
     5521 atcgagaggc acaaagccga ggtttccaga aagagccact tgttcactcc ttagcgtgac
     5581 aatcctttcc atccatttcg gcccgaaggg tcatgcgctt tagccgtttt tggctgcctt
     5641 gagcatcact tcgaggggcg cagccattcc agtccaccag gtgaaggatt cacctgcctg
     5701 gagggccagg aactcgagtc catcaacgag gggcagcccg atccggcggg cctcagccgt
     5761 ggccggcggc tcgactggac catacgcgag atcgatcagc cccgaggcgc cgtccaccac
     5821 tccgtccggc agcggctctc ccttcatccc caatggggtg gcgttgatga caatcgcgcc
     5881 cgcgaccgcc gtaccgaaag gtacgagcct gacctcgtaa ccggccttgg ccgccacttc
     5941 tcgagccagg tcgccgcgtc tggcagcaag atgaacctcc ctccccctca gggcaaccag
     6001 aacggccgca gcggcgccgc ctgcaccgag gaccaggatc ggggccgacc ggtcaaacct
     6061 ggattgtgcg agcaccgtgg ccgttgccgc ggcatccgtg gagtgcccct caaccagatc
     6121 gtcctggaat cggagggtgt tgaccgaccc tgatcggtcg gccaattcgg tcagacggtc
     6181 cgcgctcgcc gccgcaaggg ccttgagcgg cattgtgatg ttgatgccgt cgagatcccc
     6241 atctcgaagt tcgtcgaccg ctgcctcaag cccagcctgc ccgctgcgcc gcgcctcata
     6301 cgttccgctg aggccgagct ctgtgagcgc tgccctgtga attgccgggg atcgggagtg
     6361 atcgaccggg tcgccgagga gcgcaaatct caggtcggct ctcatcgccg tctgggaccc
     6421 gagtcggtgc tcagggaagg actccgtcgg cccgagcctg ctcgacattg gcgttgtgct
     6481 catcgagcgt ggctgcaaag gcatggtgcc cttcgagatc tgagagcacg taatagaggt
     6541 aatcggtggt gtccggagct gctgccgcct ccagtgatgc ccgacccggc gatgcgatgg
     6601 gggtgggtgg caggccagcg accacatagg tgttgtaggg cgagtcgacc tcgttgttga
     6661 actccgcgat gtcccgggtg tcgagtgcgt aaagcaccgt ggcatcgatg tccagcttca
     6721 tgtctatcgc caaacggttg tgaatcacgc tcgagacgat cggtcgctcc tcgtcgagga
     6781 gcacctcggt ctcaaccagc gaggcgatga tcagcccctc gtagggggtg aatccggcag
     6841 cttcgacgac agaccaatcg atgctgtcga cacgctgttc catcgtcgag gcaagccgtt
     6901 ggaggatcgc aacaggctcg gcggatttcg tgaactcgta ggtgtcgggg aagagcagac
     6961 cttcccagtc tgcgagagcc atggtcgccg gcatcgttcg cagcgaggtc gacacatccc
     7021 ggacaacaaa gcctgttcga actgctcgta gggaaccccc gacgcctcag agagccgggt
     7081 gaggatctcc cctacccgga ggccctcgat aacg
//
LOCUS       DBNHXU010000062         3633 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_218743, whole genome shotgun sequence.
ACCESSION   DBNHXU010000062 DBNHXU010000000
VERSION     DBNHXU010000062.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3633)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3633)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3633
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_218743"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="AC161X_01715"
     CDS             complement(<1..107)
                     /locus_tag="AC161X_01715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005629754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HIR4988297.1"
                     /translation="MIRRVLVANRGEIARRVFRTCRLMRIETVAIYSHP"
     gene            197..910
                     /locus_tag="AC161X_01720"
     CDS             197..910
                     /locus_tag="AC161X_01720"
                     /inference="COORDINATES: protein motif:HMM:NF025380.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4230 domain-containing protein"
                     /protein_id="HIR4988298.1"
                     /translation="MSELQVMERETETVSQPAPAPAPRRRYGFIPIAIGLGIMAFLVV
                     IGSFVVSSIRDLVGAVPTPADLVAVFEPEPFEEIGPVVISSIRDMSNLTTVETVQYTI
                     VDKGTDKGWLSWARGDSLQLFAVAKIGAGIDLSGVSVRNVSLDETGVVEISVPAAEIQ
                     YVSVDNEATQILDRETGIFTKGDARLETDARQVAEDVLVQGALDDGILTQAEDNARQV
                     LTDLLMGLGYRDVIVEFTG"
     gene            complement(918..2516)
                     /locus_tag="AC161X_01725"
     CDS             complement(918..2516)
                     /locus_tag="AC161X_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009156887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR4988299.1"
                     /translation="MQVLTTKVDPRSAEYQANRAAMEAKLAEFEALMEQARQGGGEKY
                     VTRHRERGKLLVRERIELLLDRDAPFLELSTLAAWGTDKNLGANVVTGIGVVEGVECA
                     ILAHDPTSQAGSMNRYSVAKTLRIMDIARENRLPLINLVESGGADLPDQAEVFIVGGK
                     WFRNLTRLSKQGVPTIAIVFGSSTAGGAYFPAMCDYSVMVKDQAKVFLAGPPLVKMAT
                     GEESTDEELGGAEMHSKVSGLSDYFAQDEQEAIRMGREIVSHLNWRKQGYGPTKPADD
                     PVHDIDDILGVISHDLRVPFDMREILARTVDGSRFEEYKAVYGTSILTGWASIHGFPV
                     GVVANQQGVIFSEEAQKATEFIQLCNRYDTPIVFIHNTTGYMVGKDYEQRGIIKDGAK
                     MVNVVANSEVPHITIMAGVSYGAGNYGMSGRAYEPRFVFGYPGHKVAVMGPKQLAGVM
                     SIIARQAAESRGEEFDEDEDQKRRLEIEDQIEHEEQAYYSTARVRDDGLIDPRDTRTV
                     LGIALSATHSGPVVGTDRFGVFRM"
     gene            complement(2555..2860)
                     /locus_tag="AC161X_01730"
     CDS             complement(2555..2860)
                     /locus_tag="AC161X_01730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018103739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR4988300.1"
                     /translation="MAKETKLMVWIDQDLCTGDGICEEITPDVFVGRDDGLWVVKEES
                     KYFDRTVLFDGEQAPDGARGMARIPDALIQDALESAEECPGECIMIEPYSAEAVHDG"
     gene            2906..3352
                     /locus_tag="AC161X_01735"
     CDS             2906..3352
                     /locus_tag="AC161X_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007386018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HIR4988301.1"
                     /translation="MEVIDRLSSMQGDEVGVTDWMEITQERVDAFADATDDHQWIHVD
                     PVRASAGPFGGTIAHGFLTLALTVPMAGQVELDVGQPTMAINYGLEKVRFPAPAPVGS
                     RIRARVKLVAVSEVPGGIQVNRQVTMEVEGSEKPAMVAETVSRYYY"
     gene            complement(3433..>3633)
                     /locus_tag="AC161X_01740"
     CDS             complement(3433..>3633)
                     /locus_tag="AC161X_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4988302.1"
                     /translation="VAPGLISSEMSMGIPEDVRDRMVAAIPLERMGTPEEVANLVWFL
                     LTPLSSYITGEVIVVGGGRSLS"
BASE COUNT          664 a         1141 c         1142 g          686 t
ORIGIN      
        1 tcgggatgtg agtagatcgc gactgtttct atcctcatca atcgacaggt gcgaaacact
       61 cgccgggcga tctccccgcg gttggctacc aggactctcc ggatcatcgg tgggacgagg
      121 ctaccggtgg aactaatccc gggctcacga cgtcgaatct ctgtcacccc gactacgaac
      181 cgaggaaacg cgcccaatga gcgaactaca ggtcatggaa agagagaccg agacggtctc
      241 acaaccggct ccggcacccg cgccgagacg gcgatatgga ttcatcccga tcgcgatcgg
      301 ccttgggatc atggctttcc tggtcgtgat tggaagcttt gtcgtctcga gtattcgcga
      361 cctcgtcggc gcggtgccaa cccccgccga tctggttgcg gttttcgagc ccgagccatt
      421 cgaggagatc gggccggtcg taatcagctc gatccgtgac atgtcgaacc tgacgacggt
      481 cgagaccgtc cagtacacga ttgtcgacaa gggcaccgac aaaggctggc tgtcctgggc
      541 ccgcggagac agtctccaac tgtttgcggt ggccaagatc ggcgccggca tcgacctgag
      601 tggggtctca gttcggaatg tgtccctcga cgagaccggc gtggtcgaga tcagcgtgcc
      661 cgctgcggag attcagtacg tctcggtgga caacgaggcc acccaaatcc tcgaccgtga
      721 aaccgggatc ttcaccaagg gtgatgcccg tttggagacc gacgcccgcc aggttgccga
      781 ggatgtcctg gttcagggtg ctctcgacga tgggattctc acccaggcgg aggacaacgc
      841 acgccaggtg ctcaccgact tgctgatggg tctcggctat cgcgacgtga tcgtcgaatt
      901 caccggctag gtcgagctca catccggaat acgccaaagc ggtcggtacc gaccaccggc
      961 ccggaatgag tggcagatag ggcgatgccg agcacggtgc gggtgtcacg ggggtcgata
     1021 agaccgtcat ctcgcacccg cgccgtcgag taataggcct gttcttcgtg ctcgatctgg
     1081 tcttcgatct ccagccggcg cttctggtct tcgtcctcgt cgaactcctc gcctcgtgat
     1141 tcggctgcct gacgggcgat gatcgacatg acacctgcaa gttgcttcgg gcccatcacc
     1201 gccactttgt ggccgggata accgaacacg aaacgcggct cgtaagcgcg ccccgacatc
     1261 ccgtagttcc cggcgccgta ggagacgccg gccatgatcg tgatgtgcgg cacctcggag
     1321 ttggcgacca cattcaccat cttcgcccca tctttgatga tccctcgctg ctcgtagtcc
     1381 ttgcccacca tgtagccggt ggtgttatgg atgaagacga tcggggtgtc atagcggttg
     1441 cagagctgga tgaactcggt tgccttctgc gcctcttcag agaagatcac tccctgctga
     1501 ttggcgacca cccccacggg aaacccgtgg atcgacgccc aaccggtgag gatcgaggtt
     1561 ccataaaccg ccttgtactc ctcgaatcgg gagccgtcga cggtccgggc caggatctcc
     1621 cgcatatcga aagggacccg gaggtcgtgg gatatgacac ccaggatgtc atcgatgtca
     1681 tgcacgggat catcggccgg ctttgtcggg ccgtaaccct gcttgcgcca gttcaggtgg
     1741 gagacgatct cgcgacccat ccggatcgct tcctgctcgt cctgggcgaa gtagtcggag
     1801 agccctgaga ccttggagtg catctccgcc ccgccgagct cctcgtcggt ggactcctcg
     1861 cctgtcgcca tcttgaccag gggcggcccg gccaggaaca ccttggcctg atccttcacc
     1921 atgaccgagt agtcgcacat cgccgggaag taggcgcctc ccgcggtgga gctgccgaac
     1981 acgatggcga tggtggggac gccctgcttg gagagacggg tgaggttgcg gaaccacttg
     2041 ccgccgacga tgaacacctc ggcctgatcc gggaggtcgg cgccgccgga ttcgacgagg
     2101 ttgatcaagg gcagccggtt ctcgcgggcg atgtccatga tgcgaagggt cttcgccacc
     2161 gaatagcggt tcatcgaccc ggcctggctg gttgggtcgt gggcgaggat ggcgcactcg
     2221 accccttcca ccactccaat cccggtgacg acatttgcgc ccaggttctt gtccgtgccc
     2281 cacgcggcga gagtggacaa ctcgaggaag ggggcatcac ggtcgaggag gagctcgatg
     2341 cgctcccgaa ccagcagctt cccccgctcc cgatggcggg tgacgtactt ctccccaccc
     2401 ccctgacgag cctgctccat gagagcctcg aattcggcca gcttcgcctc catggcggcg
     2461 cgattggcct ggtactccgc ggaacgggga tcgaccttgg tggtcagtac ctgcatctgg
     2521 tgccgacgag gctagttgtc agcgagccgc ccgctcagcc gtcgtggacc gcctcggcgc
     2581 tgtagggctc gatcatgatg cactcgccgg ggcattcctc tgccgattcc aaggcgtctt
     2641 ggatgagggc gtcggggatg cgggccattc cccgggcacc gtcgggtgcc tgctctccgt
     2701 cgaagaggac ggtccggtcg aagtacttcg attcttcttt cacaacccac agcccgtcat
     2761 cgcgaccgac gaacacatcg ggggtgatct cctcacagat cccgtcaccg gtgcacaggt
     2821 cctggtcgat ccagaccatg agcttggtct ctttggccat gcccccaaac taggcgattc
     2881 tcacgcttgt gtcagaatcc gtgggatgga agtgatagat cggctgagct cgatgcaggg
     2941 cgatgaggtc ggtgtcaccg actggatgga gatcacccag gagcgggtcg acgcattcgc
     3001 agacgccaca gatgaccatc agtggatcca tgtcgatccg gtccgtgcgt ccgcaggccc
     3061 cttcggcggg acgatcgccc acggatttct gacactcgca ctcaccgtgc ctatggcagg
     3121 ccaggtggag ctcgacgtcg gccaaccgac aatggcgatc aactacgggt tggaaaaggt
     3181 gcgattcccg gcgccggcgc cggtcgggtc acggatcagg gcgagggtga agttggtggc
     3241 cgtgtccgag gtcccgggcg ggatccaggt caaccgacag gtgacgatgg aagtcgaggg
     3301 aagcgagaag ccggcgatgg tcgccgagac ggtcagcagg tactactact gagtttctcc
     3361 ccccagtgaa cgaagtgagc gttgggggga gtaccgccgg aggcggggtg gggggcgccc
     3421 tgccaagcca acctacgaga gactccgtcc cccgccgacc acgatcacct ctcccgtgat
     3481 gtagctcgag agcggggtga gcaggaacca caccagattg gccacctcct cgggcgttcc
     3541 catcctctcc aaggggatcg ccgccaccat ccgatctctc acatcctcgg ggatgcccat
     3601 gctcatctcc gaagagatca gccccggcgc cac
//
LOCUS       DBNHXU010000063         2045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1211116, whole genome shotgun sequence.
ACCESSION   DBNHXU010000063 DBNHXU010000000
VERSION     DBNHXU010000063.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1211116"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC161X_01745"
     CDS             <1..389
                     /locus_tag="AC161X_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007676888.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR4988303.1"
                     /translation="IGTVKGDIHDIGKNLVAMMLEGAGFEVINLGINTDAEEFIAALE
                     EHKPDILGMSALLTTTMPYMKVVIDALKERNIRDDYIVMVGGAPLNEEFGMAIGADAY
                     CRDAAVATETAEALVLARRITSGEDP"
     gene            386..997
                     /locus_tag="AC161X_01750"
     CDS             386..997
                     /locus_tag="AC161X_01750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1638 domain-containing protein"
                     /protein_id="HIR4988304.1"
                     /translation="MSEPRDGVLVIACGALAKEVLDVVRVNSLDWVDVECLPSRLHNT
                     PDLIPAAVAERIDSGLQRYREVFVGYADCGTGGALDAVLEARGVERLPGAHCYEFFAT
                     SDVFQSLSDQEPGTFYLTDFLVRHFDRLVWRGLGLDRWPHLRDDYFGNYKRLVYLSQI
                     ETPDLIHRARAAASRLGLGFEHRPVGYGDLATSLVEVSRRVPA"
     gene            994..1305
                     /locus_tag="AC161X_01755"
     CDS             994..1305
                     /locus_tag="AC161X_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="virulence factor"
                     /protein_id="HIR4988305.1"
                     /translation="MSPRETGPELTVIRWRQIPAQVVARKGRETVKRELSPRFQVAID
                     RAAMYAGLFGTDDYLSEWARDTRPCGEDLESEVAAEVTRLESEFDPDSLGELAGNGGI
                     K"
     gene            1302..>2045
                     /locus_tag="AC161X_01760"
     CDS             1302..>2045
                     /locus_tag="AC161X_01760"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009471535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR4988306.1"
                     /translation="MTDTVVSSPTKEVVIGFDRPFVLIGERINPTGRKLLAEEMKNGD
                     FSRVETDALSQVQAGAHMLDVNAGIPLADEPALLARAVQLVQSLTDVPLSIDSSIVEA
                     LEAGLAVYQGKALVNSVTGEEERLELVLPLVAKHGAAVVAISNDETGISEDPDVRFDV
                     AKKIVNRAADYGIPTEDVVVDPLVMPIGAIATAGRQVFHLVGRLREELGVNTTCGASN
                     ISFGLPNRAGINAAYLAMVMGAGMTSAITN"
BASE COUNT          367 a          634 c          653 g          391 t
ORIGIN      
        1 tgatcggcac cgtcaaggga gacattcacg acatcggtaa gaacctggtg gcgatgatgc
       61 tcgagggtgc cggtttcgag gtgatcaacc tcgggatcaa caccgatgcc gaagagttca
      121 tcgccgccct cgaagagcac aagccggaca tcctcggcat gtcggcgctt ctcaccacga
      181 ccatgcccta catgaaggtc gttatcgacg ctctgaagga gcggaacatt cgcgacgact
      241 acatcgtcat ggtcggcggc gccccgctca acgaggaatt cggcatggcg attggtgccg
      301 acgcctattg ccgcgacgcg gcggttgcca ccgagaccgc cgaggctctt gttcttgccc
      361 gcaggatcac ctccggggag gacccgtgag cgagcctcgt gatggggtct tggtgattgc
      421 gtgcggggcg cttgccaaag aggtcctcga cgtggtccgg gtcaactctt tggactgggt
      481 tgatgtcgag tgcctgccgt cccggctcca caacacaccc gacctcatac ctgcggcggt
      541 cgcggaaaga attgactcgg gtctccagcg ctaccgcgaa gtgtttgtcg gctatgccga
      601 ctgcggaacg ggcggggccc ttgatgcggt gctcgaggcc cgcggtgtcg agcgactccc
      661 tggggcccac tgctacgaat tctttgcgac cagtgacgtt ttccagtcct tgtccgatca
      721 ggagccgggc accttctatc tgaccgactt cctggttcgc catttcgatc gcctggtctg
      781 gcgtgggctc ggcctcgatc gctggcccca cctccgagac gattacttcg gcaactacaa
      841 gcgcctggtc tatctctccc agatcgaaac ccccgatctg atccaccgtg ccagagccgc
      901 cgcctctcgt ctcggcctgg ggttcgagca ccgtcctgtt ggatacggcg atctggcgac
      961 cagcctggtc gaagtgtcgc gtcgggtccc ggcgtgagtc cccgtgagac cgggcccgag
     1021 ttgaccgtca tccgctggcg ccagattcct gcgcaggtgg tagcccgcaa agggagagag
     1081 acggtcaagc gcgagctgtc cccgagattt caggtcgcca tcgaccgtgc cgccatgtac
     1141 gccggtttgt tcggcaccga tgactacctc tcggagtggg cccgggacac gcgcccctgc
     1201 ggtgaggacc tcgaaagcga agtcgcggcc gaagtgaccc ggctcgaatc cgagttcgat
     1261 ccagacagcc tgggcgaact ggcggggaac ggaggcatca aatgaccgac accgtggtct
     1321 cctcccccac gaaggaagta gtcatcggct tcgatcggcc gttcgtcctg atcggcgaac
     1381 gaataaaccc aaccgggcgc aagcttctcg ccgaggaaat gaagaacggg gacttcagcc
     1441 gggtcgagac cgatgctctg agtcaggttc aagccggcgc ccacatgctc gacgtcaacg
     1501 ctgggatacc gctcgccgac gagccggcac tcctcgctcg ggcagttcag ttggtccaat
     1561 ccctcactga cgtccctttg tcgatcgact cctcaatcgt ggaggcgctg gaggccgggc
     1621 ttgccgtcta tcaggggaag gccctggtca actcggtgac tggtgaggag gagcgtctgg
     1681 agttggtcct gcccctcgtt gccaagcatg gggccgcggt ggtagcgatt tccaatgacg
     1741 agactgggat ctctgaggac cccgacgttc gcttcgacgt ggctaagaag atcgtgaatc
     1801 gggctgcgga ctacgggatc cccaccgagg atgtggtggt cgacccgttg gtgatgccga
     1861 ttggcgccat cgccacagcc ggcaggcagg tgttccatct ggtgggcagg ttgcgcgaag
     1921 agctaggagt caacaccacg tgcggcgcct cgaacatcag cttcgggctc ccaaatcggg
     1981 cagggatcaa cgcggcttat ctggcgatgg tgatgggggc cggcatgacc tcggccatca
     2041 ccaat
//
LOCUS       DBNHXU010000064         2869 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5179258, whole genome shotgun sequence.
ACCESSION   DBNHXU010000064 DBNHXU010000000
VERSION     DBNHXU010000064.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2869)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2869
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5179258"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2869
                     /gene="rpoB"
                     /locus_tag="AC161X_01765"
     CDS             <1..>2869
                     /gene="rpoB"
                     /locus_tag="AC161X_01765"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_037647936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR4988307.1"
                     /translation="SGKIIPGRGAWLEFDTDKKDTIGVRVDRKRRQYVSAFLKALGIA
                     ETDEEILEIFGGAQSIRNTIEKDTAADKEDALLDLYRKLRPGEPTTVESARGLIQTLF
                     RNPKRYDLTRVGRYKISQKYREESPSDYRAEGLQLLRDEDIIDAIKYLVALHAGEKTM
                     VTHKGTEVQVLTDDIDHFGNRRIRTVGELVQNQVRVGLTRLERVVRERMTTQDPDAIT
                     PQSLINIRPVTAAIKEFFGTSQLSQFMDQPNPLAGLTHRRRLSALGPGGLSRERAGFE
                     VRDVHPSHYGRMCPIETPEGPNIGLIGSLASYARVNQFGFIETPYRKVISGKVTAQVE
                     YLTAIEEERYVIAQANAPLKDDGAFKNPRVLVRKAGGEVEYIPPSEVDYMDVSPKQIV
                     SVSTSLIPFLEHDDANRALMGSNMQRQAVPLLTADAPLVGTGVERRAAQDSGDMITST
                     VDGEVVEVTGDEIVVKEKETNKKHTFPLDKFKRSNQGTCMNHKPLVREGDHVKVNDVL
                     ADGPSTEAGELALGRNLLVAFMPWQGHNFEDAIIISERLVKDDVLTSIHIEEHELDAR
                     DTKLGAEEITRDIPNVPEEVLANLDEEGIIRVGAEVGPGDYLVGKVTPKGETELTPEE
                     RLLRAIFGEKAREVRDTSLKVPHGETGKVIAVKVFKRAEGYDLPPGVNELVRIYVAQQ
                     RKISEGDKLAGRHGNKGVISKVLAEEEMPFMADGTPVDIILSPLGVPSRMNLGQVLET
                     HLGWAAAQGWEPFKGESPAVVGAKPWTKVATPVFDGAREDEIMQVISSARTNRDGERL
                     VADDGKAILYDGRSGEPFDTPITIGFIYMLKLVHLVDEKIHARSTGPYSMITQQPLGG
                     KAQFGGQRFGEMEVWALEAYGAAYALQELLTIKSDDVQGRVKVYEAIVKGDNIPEPGI
                     PESFRVLVKEMQSLCLNVEMLAAGEEVELRELEEESYRSVET"
BASE COUNT          601 a          902 c          879 g          487 t
ORIGIN      
        1 tccggcaaga tcatcccggg acggggggca tggctcgagt tcgacaccga caagaaggac
       61 accattggtg ttcgtgtcga tcgcaagcgg cgccagtacg tgtcggcctt cctaaaggcg
      121 cttggcattg ccgagaccga cgaggagatc ctcgagatat tcggtggtgc gcaatcgata
      181 agaaacacga tcgagaagga caccgccgca gacaaggaag acgcccttct cgacctctat
      241 cgcaagctgc gacccggtga gccaaccacc gtcgagtcgg cccgtggcct gatccagacg
      301 ttgttccgca accccaagcg ctacgacctg acccgtgtcg ggcgttacaa gatcagccag
      361 aagtaccgcg aggagtcgcc ctccgattac cgcgccgaag gcctccagct gctgcgagac
      421 gaagacatca tcgatgcaat caagtatctg gtcgcccttc acgccggcga gaagacgatg
      481 gtcacccaca agggaaccga agtccaagtg ctcaccgacg acatcgacca cttcggtaac
      541 cgccgtatcc gcactgttgg tgagttggtc cagaaccagg tccgtgtcgg gctgacccgt
      601 ctcgagcgcg tggtccggga gcggatgact actcaggatc ccgacgccat caccccccag
      661 agcctgatca acattcgccc ggtcaccgcg gcgatcaagg agttcttcgg gaccagccag
      721 ctcagccagt tcatggacca gcccaacccc cttgccgggc tcacccaccg tcgccgtctc
      781 tcggcgctcg gcccgggcgg tctgtccagg gagcgggcag ggttcgaggt ccgtgacgtt
      841 cacccgtccc actacggccg gatgtgcccg atcgagactc ccgagggccc gaacatcggc
      901 ctgatcggat cccttgcctc atatgcgcgg gtcaaccagt tcggcttcat cgaaactcca
      961 tatcgcaagg tgatcagcgg aaaggtcacc gctcaggtcg agtacctgac ggctatcgaa
     1021 gaagagcgct acgtcatcgc gcaggccaac gccccgctca aggacgacgg tgccttcaag
     1081 aatcctcggg ttctcgtgcg caaggcaggt ggcgaggtgg agtacatacc tccctcggag
     1141 gttgactaca tggacgtctc tccgaagcag atcgtttcgg tctctacatc gttgatcccg
     1201 ttcctggagc atgacgacgc caaccgcgcc ctgatgggct cgaacatgca acgtcaggca
     1261 gttcctctgc tcaccgccga tgcgccactc gtgggcaccg gggtcgagcg ccgcgccgcc
     1321 caggactcgg gcgacatgat cacctcgact gtcgatggcg aagtcgtcga ggtgaccggt
     1381 gacgagatcg tggtcaagga gaaggagacc aacaagaagc acaccttccc cctcgacaag
     1441 ttcaagaggt cgaaccaggg cacctgcatg aaccacaaac cactggtgcg cgagggcgac
     1501 catgtgaagg taaatgacgt gctggccgac ggcccctcga ccgaggccgg ggaactagcg
     1561 ctcggacgca acctcctggt cgcattcatg ccgtggcagg gtcacaactt cgaggacgca
     1621 atcatcatct ccgagcgcct cgtcaaggac gacgttctca cctctattca catcgaagag
     1681 cacgagctcg acgcccggga caccaagctc ggcgccgagg agatcaccag ggacatcccg
     1741 aatgtccccg aggaggtcct cgccaacctc gatgaagagg gaatcattcg cgtcggcgcc
     1801 gaagttgggc ctggcgacta tctcgtcggc aaggtcactc ccaagggcga gaccgagctg
     1861 acgccagagg agcgcctgct tcgggcgatc tttggagaga aggcgcgcga agttcgtgac
     1921 acttccttga aggttcccca cggcgagacc ggaaaggtca tcgcggtcaa ggtgttcaag
     1981 cgagccgagg gttatgacct tccgcccgga gtcaacgagt tggtgcgcat ctacgtcgcc
     2041 cagcagcgca agatctccga gggcgacaag ctcgccggcc gacatggcaa caaaggcgtg
     2101 atctccaagg tcctcgccga ggaggagatg ccattcatgg ccgacgggac ccccgtcgac
     2161 atcatcctct ccccactcgg tgtgccttcc cgtatgaacc tgggccaggt gctcgagacc
     2221 catctcgggt gggctgccgc ccaaggttgg gagccgttca agggtgagag cccggcggtt
     2281 gttggtgcca agccgtggac caaggtggcg acaccggtct tcgacggtgc ccgtgaagac
     2341 gagatcatgc aggtcatcag cagcgctcgg acgaatcgcg acggcgagcg cctcgttgcc
     2401 gacgacggca aggcgatcct ctatgacggt cgcagcggtg agcctttcga cactccgatc
     2461 accatcggct tcatctacat gctgaaactg gtccacctgg tcgacgagaa gatccatgca
     2521 aggtcgaccg gcccgtacag catgatcacc cagcagcctt tgggcggtaa ggcccagttc
     2581 ggtggccagc ggtttggaga gatggaggtc tgggcactcg aggcctatgg agccgcctat
     2641 gcactccagg agctgctcac catcaagtcc gacgacgtgc aggggcgggt caaggtctac
     2701 gaggcaattg tcaagggcga caacatcccc gagcctggga tcccggagtc gttccgggtt
     2761 ctggtcaagg agatgcagag cctctgtctc aatgtcgaga tgttggccgc cggtgaggaa
     2821 gtcgagctcc gtgagctcga ggaagagagt taccgctcgg tcgagaccc
//
LOCUS       DBNHXU010000065         4661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4782201, whole genome shotgun sequence.
ACCESSION   DBNHXU010000065 DBNHXU010000000
VERSION     DBNHXU010000065.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4661
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4782201"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1330
                     /locus_tag="AC161X_01770"
     CDS             <1..1330
                     /locus_tag="AC161X_01770"
                     /inference="COORDINATES: protein motif:HMM:NF014605.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HIR4988308.1"
                     /translation="TLKEIIEVADTFVLDTCVLLADPQSPLRFDEHDVVLPLVVIEEL
                     DRKKTRMDEVGANARAAIRLIEELGASRQGGMREPVPLPGGGTLRIELNGITSERLPA
                     ALDPQTPDHRILATCLNLVEKTAAVLVTKDAALRIKGAQLGVAVQDYRADTVPVGQLQ
                     SGLIEHFTEGEVIDRLFDEGKIDLQGVEAEVNQFIVLKAGTSHSALARVIESDPVVIV
                     ERVPTHVRAFGVEPKNVRQTVALHQLLDPDIPAVSVMGMAGTGKTFLALAAALEQVVE
                     QGRYRRVSVYRPLVAVGRQEVGFLPGDLNEKLAPWMAAVHDNLYALFSDAGVDGARHA
                     IDELVDRGELEMAAVTYLRGRSITGEFVIIDEAQNLELPTLKVILTRMSRDSKVVFCG
                     DLSQVDNPYISPFGGMAALIERFKGSPLFGHITLERSVRSPLAEMAANIL"
     gene            1339..1533
                     /locus_tag="AC161X_01775"
     CDS             1339..1533
                     /locus_tag="AC161X_01775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988309.1"
                     /translation="MTEPEKGEDRDKPGRRGSLVGVGAVLGVGIGAAVGVAIDQMSTG
                     IWIGAVIGVGLGMLASYFQK"
     gene            complement(1549..3174)
                     /locus_tag="AC161X_01780"
     CDS             complement(1549..3174)
                     /locus_tag="AC161X_01780"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4988310.1"
                     /translation="MAAIAFGFGFALVAGKPLQDVNTSQVLYASAAVASGLILIAAAI
                     IANGRSRIAWSLIGTGVLFWGIGEAVWMIQSRAGEIPYPGIADFFYVAGYPLIFAGVI
                     VLPYLRPGRFERIRLAIDATAGTLSLAVVMWVAYLHDVVALGTEPVETFLNVGYPLGD
                     ILLMTALMVLAMRRSEQRLDLRILLLAAGVALTAVADVAFSLQSASGTYVEWGWIDGL
                     YLFSYGFLALSAWFVTRPAPSQETSYRSLNTWQLIAPYTAVATLFLVRLFTSSGSDLL
                     LNLATTAVAILVVVRQNLAVREKKELLERQRDDLVASVSHELRTPLTGIQGYAEILSD
                     SWEMLTNEERAEMLDTINTQANHLGHIVTDLIDVARDRLQNVKLNRVDYGAADIVREA
                     VAASGAGRDIRIAAESEARVWADPDRVRQVLVNLITNAIRYGHSEILVTAARTGGMVT
                     FAVHDDGEGVPAKYQADIWERFERGFHKFNTSEPGSGIGLSVAKGLVTAHGGTIGYRT
                     SELLGGACFEFTIPTADHHEPIAVFGGRVERVG"
     gene            complement(3321..>4661)
                     /locus_tag="AC161X_01785"
     CDS             complement(3321..>4661)
                     /locus_tag="AC161X_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006542991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HIR4988311.1"
                     /translation="MADAVGFTVSAKLTATSAEGVKATFSTSGRTITFPGFLRAYVQG
                     ADDPESDLDDREEILPPLTEGEALAAQRLEPTGHETRPPARYTEASLIQRLEELGVGR
                     PSTYASIMSTIQDRGYVWKRGTALVPTFTAFGVVSLLEHHFPNLVDYAFTAEMENDLD
                     RIAAGEEETVPWLTAFYFGSDGLKERVATRLDEIDPRAIGSIPIGEDKSGQPVIARIG
                     RYGPYVERGEERASIPEDVAPDELTVSRAIELLETQNAGDKIFGTDPDSGLTVYGRTG
                     RFGPYVQLGEVEEGSKEKPKRASLLKTMSLDELSMDDALRLLSLPRTVGIDEAGVEIV
                     ALNGRYGPYIQRGTDRRSLEAEEQLFTLTVDEALTLLAEPPRRRGQRNSGPLKELGAD
                     PVSGKPVTVRSGRFGPYVTDGEVNASLRQGDSPESMTLERAAELLAGRRERIGG"
BASE COUNT          855 a         1452 c         1516 g          838 t
ORIGIN      
        1 gaccctgaag gagatcatcg aggtggccga cacttttgtc ctcgacacct gcgtcctgct
       61 cgccgacccc caatcccccc tccgcttcga tgaacacgat gtggtcctgc ccctggtcgt
      121 catcgaagag ctggatcgga agaagacccg gatggatgag gtcggcgcca atgctcgagc
      181 cgcgatcagg ctcatcgagg agctcggcgc ctcccgacag ggtgggatgc gagaacccgt
      241 gcccctcccc ggtggcggga cgcttcgaat cgagctcaac gggatcacct cggagcgcct
      301 gccggcagct ctcgatcccc agacacccga tcaccggatc ctggccacct gcctcaacct
      361 ggtcgagaag acggcggcgg tgctggtgac caaggacgcg gcattgcgga tcaaaggggc
      421 gcaactgggt gtggcagttc aggactaccg ggccgacacg gtccccgtgg ggcaactgca
      481 atcagggctg atcgagcact tcaccgaggg tgaggtcatc gaccgcctct tcgatgaggg
      541 gaagatcgac ttgcaggggg ttgaggccga ggtcaatcaa ttcatcgttc tcaaagccgg
      601 tacctcgcat tcggcgctgg cacgggtcat cgaatccgac ccggtggtca tcgtcgagcg
      661 agtgccgacc catgtccggg cgttcggcgt cgagccgaag aacgtccgcc agaccgtggc
      721 cctgcaccaa ctgctggatc cggacattcc cgctgtttcg gtgatgggga tggcgggcac
      781 cggcaagacg ttcctcgctc tggcggccgc tttggagcag gtagtcgaac agggcaggta
      841 ccgccgggtt tcggtctacc ggccattggt cgcggttggg cgccaggaag tcggcttcct
      901 gcccggtgat ctcaacgaga agctcgcccc atggatggcc gcagttcacg acaacctcta
      961 cgccctcttc tcagacgcgg gcgtcgatgg ggcccggcac gccatcgacg agttggtgga
     1021 ccgtggcgag ctcgaaatgg cagccgtcac ctatctgcgg gggcgctcga tcaccgggga
     1081 gttcgtgatc atcgacgagg cccagaacct cgaattgccg accctcaagg tgatcctgac
     1141 ccggatgtca cgggactcca aggttgtgtt ctgcggggac ctatcccagg tcgacaatcc
     1201 ctacatctcg ccttttggcg ggatggcagc cttgatcgaa aggttcaaag gctcgccgct
     1261 gttcgggcac atcacccttg agcggtccgt acgctcaccc ctggcagaga tggcagccaa
     1321 catcctctag gaggaatcat gaccgagccg gagaagggtg aggatcgcga caagccaggt
     1381 cgccggggat cgttggtcgg tgtcggcgcg gtgctgggcg tcgggatcgg cgccgcagtg
     1441 ggcgtagcga tcgatcaaat gtcgaccggg atctggattg gcgctgtcat tggcgtcggg
     1501 ctggggatgt tggccagcta tttccagaaa tagccaacca agctcgcttc agccaactcg
     1561 ctccactcga ccgccaaaaa ccgctatcgg ctcatggtga tcggcggttg ggatcgtgaa
     1621 ttcgaagcac gctcccccga gaagctccga agttcggtag ccgatggtcc cgccgtgagc
     1681 ggtgaccaag cccttcgcca ctgacaaccc gatccccgag ccgggctcgc tcgtgttgaa
     1741 cttgtggaat ccccgttcga accgttccca gatgtcggcc tggtacttgg cggggacgcc
     1801 ttcgccgtcg tcatggaccg caaacgtcac cattcccccg gtgcgggcgg ccgtgaccag
     1861 gatttcggag tgaccgtatc ggatagcgtt tgtgatcagg ttgacgagaa cctggcggac
     1921 tcggtccgga tcggcccata ctcgagcttc ggattcggca gcaatacgga tgtcgcgacc
     1981 ggcaccgctg gctgcaaccg cttcccggac gatgtcggcg gcgccgtagt cgacccggtt
     2041 cagcttcacg ttctgcaggc gatcgcgggc aacatcgatc aaatcggtca cgatgtgacc
     2101 gagatggttg gcctgggtgt tgatcgtgtc caacatctcg gcgcgctctt cgttggtcaa
     2161 catctcccat gagtcggaca ggatctcggc atagccctgg atcccggtca gtggagttcg
     2221 caactcgtgt gacaccgagg cgacgagatc gtcacgctgg cgctccagca gctccttctt
     2281 ttcacgaact gcaaggttct ggcggaccac caccaggatg gccactgcgg tggttgcgag
     2341 gttgagcagg aggtcgctcc ccgaactggt gaacagccgc accaggaaca gagtcgccac
     2401 cgccgtatag ggcgcgatca actgccaggt gttcaacgag cgatagctgg tctcttgcga
     2461 tggcgctggc ctggtgacga accaggcgga cagggcgagg aacccatagc tgaagaggta
     2521 caagccatcg atccagcccc actcgacgta ggtgcccgaa gcgctttgga gcgagaaggc
     2581 gacatctgcc acggcagtga gcgctacacc ggctgccagc agaaggattc ggaggtcgag
     2641 acgctgttcg gacctccgca tggcaagaac catcaacgcc gtcatcaaga ggatgtcgcc
     2701 cagcgggtag cccacattga ggaaggtctc gaccggttcg gtgcccaacg ccacaacgtc
     2761 gtggagatag gcgacccaca tgaccacggc caaggacaag gtccccgcgg tggcatcgat
     2821 cgccagccgg atgcgctcga accgccccgg ccgaagatag gggaggacga tcacgccggc
     2881 gaatatcaac gggtagccgg ccacatagaa gaagtcggcg atcccgggat aggggatctc
     2941 cccggcccgg ctttgaatca tccacactgc ctcgccgatc ccccagaaca gcacgcccgt
     3001 gccgatcaga ctccaggcga tccgggaacg gccgttggca atgatcgccg cagcgatcag
     3061 gatcagaccc gatgcgaccg ccgcggacgc gtagaggacc tgtgaggtgt tgacgtcctg
     3121 aagtggcttg ccggcaacca gggcgaagcc aaagccaaag gcaatcgcgg ccacgaaaat
     3181 cgaggccgct cgtgtggcag ctgcccaagt cgatcgggtc ggggatacat acgagatact
     3241 gatcggctcc tgtatctaga agatcggccc ccagcgccac gaagttgagg gtccccaggc
     3301 ccaaaggccg gtgggcctgg tcagccgccg atgcgctcgc gacgccccgc aagcaactcg
     3361 gctgctcgct ccaaggtcat gctctccggc gagtcacctt gacgcaggga cgcgttcacc
     3421 tcgccatcgg ttacatatgg gccgaaacgg cccgagcgga cggttacggg cttgccgctg
     3481 acggggtcgg cgccaagttc cttcagaggc ccggaattgc gctggccccg tcgccggggc
     3541 ggctcggcaa ggagggtgag cgcctcatcc acggtcagtg tgaacagctg ctcctccgct
     3601 tcgagactgc gtcgatcggt gcccctttgg atataggggc cgtaccggcc gttaagggcg
     3661 acgatctcca cgccggcctc gtcgatgcca accgtgcggg gtagggagag aaggcggagg
     3721 gcgtcgtcca tggaaagctc gtctaaggac atcgtcttga ggagcgaggc ccgcttcggc
     3781 ttttccttgc tgccttcctc gacctcaccg agttggacgt aaggcccgaa gcgtccggtt
     3841 cggccgtaga ccgtaagccc gctgtcgggg tcggtcccga agatcttgtc cccggcgttc
     3901 tgcgtctcca agagctcgat ggctcggctt acggtgagct cgtctggggc gacgtcctct
     3961 gggatcgagg cccgctcctc tccccgctcc acgtaaggcc cgtatcggcc aattcgtgcg
     4021 atgacgggct gtccactctt gtcttcaccg atcgggatcg acccgatggc tcgggggtcg
     4081 atctcgtcga ggcgtgtggc gacccgctcc ttgagcccgt cgctcccgaa atagaaggca
     4141 gtgagccagg gcacggtctc ttcctcaccg gccgcaatcc ggtcgaggtc gttttccatc
     4201 tccgcggtga atgcgtagtc gacgagattg gggaagtggt gttcgagcag ggaaaccacc
     4261 ccgaaggcgg tgaaggtggg gacgagggcg gttccccgct tccagacgta tccccggtcc
     4321 tggatggtcg acatgatcga tgcataggtg gacggcctgc ccactccgag ctcttcgagc
     4381 ctttggatga gggaggcctc cgtgtagcga gcgggcggcc tggtctcgtg cccggtgggc
     4441 tcgaggcgtt gcgcggccag agcttcgcct tcggtcagcg gcgggaggat ctcctcgcga
     4501 tcgtcgagat cggactcagg gtcgtcagct ccctgcacgt aagcgcgcag gaaaccaggg
     4561 aaagtgatgg ttcgcccgct ggtgctgaag gttgccttga ccccctcggc gctggtcgcg
     4621 gtcagcttcg ccgacacggt gaagcccacc gcatcggcca t
//
LOCUS       DBNHXU010000066         5499 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3591593, whole genome shotgun sequence.
ACCESSION   DBNHXU010000066 DBNHXU010000000
VERSION     DBNHXU010000066.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5499)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5499)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5499
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3591593"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..486
                     /locus_tag="AC161X_01790"
     CDS             <1..486
                     /locus_tag="AC161X_01790"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR4988312.1"
                     /translation="LLVGNLFSVTPLINFPITSRMAIPAFLSLVTYWIFVIVGFSKNH
                     FKYLIDTAWPRAVPIGLRWVVGLIEVVSIFLLRPITLAVRLFANMVAGHLMLTLLLGS
                     GVIFVAAIPEIGVKGTIGVFWFALGLFMYLLEILIAVLQAYIFTLLTAIYIESSIHFE
                     H"
     gene            500..733
                     /gene="atpE"
                     /locus_tag="AC161X_01795"
     CDS             500..733
                     /gene="atpE"
                     /locus_tag="AC161X_01795"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014010346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR4988313.1"
                     /translation="MEGNIEALTGALALIGYGLGAIGPGIGIGIVAGNAITAMARQPE
                     MQGTIRTTMFLGIALTEALALIGFVLFFIASAG"
     gene            742..1290
                     /gene="atpF"
                     /locus_tag="AC161X_01800"
     CDS             742..1290
                     /gene="atpF"
                     /locus_tag="AC161X_01800"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR4988314.1"
                     /translation="MHFLNALTVLAQEEETSGGLSLVLPHPEELIAGILAFLIVFFFV
                     WKWALPTINKTLEARQQAITAQIDEAEAAKREAETLLTDYKAQLADSKAEGNRIVEEA
                     RVAGDQMKSEIISKANDEAEQLIAKARTEADTEKSRALADARREVGELSVELAGKIVG
                     ESLDPKAHQDLVDRYLADLEKM"
     gene            1292..1825
                     /gene="atpH"
                     /locus_tag="AC161X_01805"
     CDS             1292..1825
                     /gene="atpH"
                     /locus_tag="AC161X_01805"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit delta"
                     /protein_id="HIR4988315.1"
                     /translation="MDDRTVSYANGILELARAEGEADRVENELFEIAQALDKSPELRS
                     ALTDPQLPIERKQAVIADLLGARASATTVGIVQYIVSQGRISELPDVATALVEKAAEA
                     RSKALAEVRSAVPLDDITIERLTTALSKATGKSVEVKVIVDESVIGGIVARVGDTVID
                     GSIARRVDSLRQALNTR"
     gene            1833..3374
                     /gene="atpA"
                     /locus_tag="AC161X_01810"
     CDS             1833..3374
                     /gene="atpA"
                     /locus_tag="AC161X_01810"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HIR4988316.1"
                     /translation="MTDLTITADQITETLRSQLESWSPEVGKETVGYVTSIADGVARV
                     EGLPSVMASELLEFPGGLSGVTLNIDEDDLGVVLLGESSHIQQGDPVKQTGTLLSVPV
                     GDALLGRVIDPLGNPLDGKGPINTTERRLLETQAPSVVQRQPVNQPLQTGIKAIDAMT
                     PIGRGQRQLIIGDRQIGKTAILVDTIINQKVNQGTADAVKCIYVAIGQKASTVAEVVA
                     VLEEAGAMEYTVVVYAAASSAPALQMYAPYSGSAIGQHWMYDGQHALVVFDDLSKQAV
                     AYREISLLLRRPPGREAYPGDVFYLHSRLLERCAKLSDELGGGSLTGLPVIETKANDI
                     SAYIPTNVISITDGQCYLQSDLFYQGIRPALDAGISVSRVGGAAQISAMKKIAGPLRL
                     NLAQFRELAAFAEFGSELDAVSLAQLERGRRVVEVLKQPQFSPVPVEEQVLAIYAVTE
                     GHMDDVAIGDVARFELGLREFFRSRHTSLLGTIADSGKLPEAADMDSAIAAYKETFEG
                     SLIEE"
     gene            3382..4260
                     /locus_tag="AC161X_01815"
     CDS             3382..4260
                     /locus_tag="AC161X_01815"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006603089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HIR4988317.1"
                     /translation="MAGAQLRDTRRRIRGIESTKKITRAMELIAASRIPKAMARVQGS
                     KPYTEKLIEVIENVSAAQGGTSHRLLERREVQTVGLLIVTSDRGLAGAYASNIIRLAE
                     ARIIQLAKEGKDVLLYTVGKKAQSYFRYRGYRIEQAYVGVTDTPGYGDARAVASRVMS
                     SYAEHHIDALETFFTNFRTAMTQVPTRLDLLPITPPDRSGAETGQISYQFEPSPEEIL
                     SRLLPRYVEATIFNILLEASASEHSARRRAMKAATDNAEELIRVLSVQANRARQAEIT
                     TAITEIVGGAEALKEK"
     gene            4292..>5499
                     /gene="atpD"
                     /locus_tag="AC161X_01820"
     CDS             4292..>5499
                     /gene="atpD"
                     /locus_tag="AC161X_01820"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018224440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HIR4988318.1"
                     /translation="MSDTLSVGRIVKVAGPVVDAEFPPDGLPEILFAVEIPITIGGET
                     RTILAEVAQHLGGGKIRAIALAPTDGLTRGTEVRNTGGPITVPVGDQTLGHIFNVWGD
                     SLDAPDQDFSEPRWPIHRTPPAFEDVEPQKTVFETGIKVLDLICPYLQGGKIGLFGGA
                     GVGKTVLIQEMINRVATQHGGVSVFAGVGERTREGNDLYLEMQESGVIEKAALVFGQM
                     DEPPGVRLRVALSALTMAEYFRDVQRQDVLLFIDNIFRFTQAGSEVSTLLGRMPSEVG
                     YQPNLADEMGFLQERITSLKGRSITSLQAIYVPADDITDPAPHTAFAHLDATTVLSRP
                     LTALGIYPAVDPLDSSSRALDPLIVGADHYSVATESQRILQRYKDLQDIIAILGIDEL
                     SEEDKLVVARA"
BASE COUNT         1044 a         1769 c         1689 g          997 t
ORIGIN      
        1 cttctcgtcg gcaacctgtt ctcggtcacg ccgctgatca actttccgat cacctcacgg
       61 atggcgatcc ccgcctttct ctccctggtc acctactgga tcttcgtgat cgtcggtttc
      121 tccaagaatc acttcaagta tctgatcgac accgcgtggc ccagggccgt ccccataggc
      181 ctcaggtggg tggttggctt gatcgaagtg gtctcgatct tccttctgcg accgatcacg
      241 ctggcggtgc ggcttttcgc caacatggtc gccgggcatc tgatgctgac actgctcttg
      301 ggatccggtg tgatcttcgt agccgccatc cctgagatcg gggtcaaagg cacgatcggg
      361 gtcttctggt tcgccctcgg cttgttcatg tacctgctgg agatcctgat cgccgtgctc
      421 caggcctaca tcttcaccct cttgaccgcc atctacatcg aatcgtccat ccacttcgag
      481 cactaaggag aaagagagaa tggaaggcaa tatcgaggcc ctcaccggcg cacttgccct
      541 gatcggatac ggtctgggcg ccattggccc ggggattggc atcggaatcg tggccggcaa
      601 cgccataacc gcaatggctc gtcagcctga gatgcagggc accatccgga ccaccatgtt
      661 cctgggcatc gccctcaccg aggccctggc cctgatcggg ttcgtcctct tcttcatcgc
      721 ctcggctgga taggaaccgt catgcatttc ttgaatgccc tgaccgtgct ggcccaggag
      781 gaggagacct ccggtggcct cagcttggtg ctgccccacc ccgaggagtt gatcgcgggc
      841 atcctggcgt tcctcatcgt cttcttcttt gtctggaagt gggcgctgcc gacgatcaac
      901 aagacgctcg aggcccgtca gcaggcgatc accgcccaga tcgacgaggc cgaggctgcc
      961 aagcgagagg cggagaccct cctcaccgac tacaaggctc aactcgccga ctccaaggcc
     1021 gaaggcaacc ggatagtcga ggaggcccgg gtcgccggcg accagatgaa gtcggagatc
     1081 atctccaagg ccaacgacga agcggaacag ctgatcgcca aggctcggac cgaggcggac
     1141 accgagaaga gccgagccct tgctgatgcc cgacgcgaag ttggggagct gtcggtcgag
     1201 ctcgccggca agatcgttgg cgaatcactc gatcccaagg cccaccagga cctggtcgat
     1261 cgttacctcg ccgacctgga gaagatgtag gttggacgac cggactgtct cgtacgcaaa
     1321 cgggatcctc gagctcgcta gggccgaggg ggaagccgac cgcgtcgaga atgagctctt
     1381 tgagatcgcc caggcgctcg acaagtctcc cgagctccgg tccgcactga ccgacccgca
     1441 gttgcccatc gagcgcaagc aggccgtgat tgccgacctt ctcggagcgc gcgcctctgc
     1501 gaccaccgtc ggaatcgttc agtacatcgt cagccagggc cgcatttcgg agctgccgga
     1561 cgtggccacg gctcttgtcg agaaggccgc cgaagcccga tccaaggcat tggctgaggt
     1621 tcgttccgca gttcccctgg acgacatcac catcgagcgg ctgactaccg cgctgagcaa
     1681 ggcaaccggc aagagcgtcg aggtgaaggt gatcgtcgac gagtccgtga ttggcgggat
     1741 cgttgcccgg gttggagaca ccgtcatcga cggctcgata gcccgccgcg tcgattcgct
     1801 ccgccaggcc ctgaacactc gctaggagac caatgacaga cctgactatc accgcagacc
     1861 agataacgga gacgcttcgc agccagctgg agagctggtc gccagaggtc ggcaaggaga
     1921 cggttggtta cgtcacgtcc atcgccgacg gggttgcccg tgttgaaggc ctcccgagcg
     1981 tcatggcctc tgagcttctc gaattccccg gcggtctgag cggagtgacc ctgaatatcg
     2041 acgaagacga cctcggggtg gtgctcctcg gcgagtcgag ccatattcag caaggtgatc
     2101 cggtcaagca aacgggaacg ctgctctcgg tgcctgtcgg tgacgccctc ctcggccggg
     2161 tgatcgatcc gcttggcaac ccgctcgacg gcaaggggcc gatcaacacc acagagcgca
     2221 ggctcctcga gacccaggcc ccgagtgtcg tccagcgcca gccggtcaac cagcctttgc
     2281 agaccggcat caaagcgatc gacgcgatga ctccgatcgg gcgcggtcag cgacagctga
     2341 tcatcggcga ccgccagatc ggaaagaccg cgatcctcgt cgacaccatc atcaatcaga
     2401 aggtcaatca ggggacagcg gacgcggtca aatgcatcta cgtggccatc ggtcagaagg
     2461 cttcgaccgt ggccgaggtg gttgcggttc tcgaggaagc cggagcgatg gagtacaccg
     2521 tggtggttta tgccgccgcc tcctctgcgc cggccctcca gatgtacgcc ccctactccg
     2581 gatcggccat cggccagcac tggatgtatg acgggcagca cgccctggtc gtattcgatg
     2641 acctctcgaa gcaggctgtt gcctaccgcg agatctccct gctcctgagg cgccccccag
     2701 gtcgcgaggc gtatcccggc gatgtcttct atctccacag ccgtctcctg gagcgttgcg
     2761 caaagctctc cgatgagctc ggtggcggct cgctgaccgg tcttcccgtg atcgagacca
     2821 aggccaacga catctcggcc tacatcccga ccaacgtcat ctccatcacc gacgggcagt
     2881 gttacctgca gtccgacctc ttctatcagg ggattcgccc tgctctcgac gctgggatct
     2941 cggtctcccg ggtcggtggc gcggcgcaaa tcagcgccat gaagaagatc gccggaccgt
     3001 tgcgcctcaa cctggcccag ttccgcgagc tggcagcgtt cgccgaattc gggtccgaac
     3061 tggatgccgt ctccctggcc cagctcgaac gtgggcggcg ggtggtcgag gtgctcaagc
     3121 agccacagtt ctcaccggtg ccggtcgagg agcaggtgct tgcgatctat gcggtcaccg
     3181 aagggcacat ggacgatgtc gcaatcggcg acgttgcccg tttcgagctc ggtctgcgtg
     3241 aattcttcag gtcccgccac accagcctcc tcggcacgat tgccgacagc ggcaagctgc
     3301 ctgaggccgc tgacatggac agcgccatcg ccgcctacaa ggagaccttc gagggctccc
     3361 tgatcgagga gtgacgggtt agtggcaggc gcccaactcc gtgacacccg acgtcgtatc
     3421 cgaggcatcg agtcgacgaa gaagatcacg cgcgccatgg agctgatcgc cgccagcagg
     3481 atcccgaagg cgatggctcg ggttcagggc tccaagcctt acaccgagaa gttgatcgag
     3541 gtcatcgaga acgtgagtgc cgcccaaggc gggacatcgc atcggttgct ggagcggcgt
     3601 gaggtccaaa ccgtaggcct gctgatcgtc acctcagacc gtggcctggc cggcgcctac
     3661 gcatcgaaca tcatcaggct ggccgaagcg cggatcatcc aattggcaaa agagggcaag
     3721 gatgtgctcc tctacacggt gggcaagaag gcgcagagct acttccgcta tcggggatac
     3781 cgcatcgagc aggcctacgt gggagtcact gacactcccg ggtatggcga cgcccgcgcc
     3841 gttgcctcac gggtgatgag ctcatacgcc gagcaccaca tcgacgctct ggagaccttc
     3901 ttcaccaact tccggacggc aatgactcag gtccccaccc ggctcgatct gctccccatc
     3961 acccctcccg atcggagcgg ggcggagacg gggcaaatct cctatcagtt cgagccgtct
     4021 ccggaggaga tcctgagccg tctcctgccc aggtatgtcg aggccacgat cttcaacatc
     4081 ctcctcgagg cctcggcatc ggagcacagc gcccggcggc gggcgatgaa ggcggccacc
     4141 gacaacgcgg aagaattgat cagggtgctc agcgttcagg cgaaccgggc acggcaggcc
     4201 gagatcacca cggcgatcac cgagatcgtc ggcggtgccg aggcattgaa agagaagtga
     4261 agatgaaacc cagaagtgaa cggagtgagc aatgagtgac accttgagtg tcggccgaat
     4321 cgtcaaggtg gccggcccgg tcgtcgacgc cgagtttccg cccgatgggc tgcccgagat
     4381 cctcttcgcg gtcgagattc cgatcacgat tggcggagag acacggacaa ttctcgccga
     4441 ggtggcgcag cacctgggcg gcggcaagat cagggcgatt gccttggccc cgactgacgg
     4501 cctgacccgc ggcaccgaag tgcgcaacac cggcggcccg atcaccgtcc cggtgggcga
     4561 ccaaactttg ggtcacatct tcaacgtttg gggtgactca ctcgacgcac cggatcagga
     4621 cttctccgag ccccggtggc cgatccatcg caccccgccg gccttcgagg acgttgagcc
     4681 ccaaaagaca gtgttcgaga ccgggatcaa ggtcctcgac ctgatctgcc cttaccttca
     4741 gggtgggaag atcgggctgt tcggtggtgc gggtgtgggc aagaccgtcc tcatccagga
     4801 gatgatcaac cgggttgcca cccagcacgg tggtgtctct gtgttcgccg gcgtaggaga
     4861 gcgaacgagg gaagggaacg acctctacct cgagatgcag gagtccggtg tcatcgaaaa
     4921 ggcagcgttg gttttcgggc agatggacga gccaccgggg gtccggctcc gtgtcgccct
     4981 gtcggcgttg accatggccg aatacttccg tgacgtgcaa cgccaggacg tgctgttgtt
     5041 catcgacaac atcttccgtt tcacccaggc cggctcagag gtctcaaccc tcctcggtcg
     5101 gatgccatcg gaggtgggtt accagcccaa cttggccgac gagatggggt tcctccagga
     5161 gcggatcacc tccctaaagg gccggtcgat cacctcgctg caagcgattt atgtccctgc
     5221 agatgacatc accgacccgg cgccgcacac cgccttcgcc cacctcgatg ccaccacggt
     5281 gctctcacgg cccctgactg cgctcgggat ctacccggct gtggatccgc tcgactcgag
     5341 cagccgtgcc ctcgatccgt tgatcgtcgg tgcggatcac tactccgtgg ccaccgagtc
     5401 gcagcgaatc ctgcagcggt acaaggacct ccaggacatc attgccatcc tcggcatcga
     5461 cgagctttcc gaggaggaca agctggtggt ggcccgggc
//
LOCUS       DBNHXU010000067         2752 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_418254, whole genome shotgun sequence.
ACCESSION   DBNHXU010000067 DBNHXU010000000
VERSION     DBNHXU010000067.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2752)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2752)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2752
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_418254"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2644)
                     /locus_tag="AC161X_01825"
     CDS             complement(<1..2644)
                     /locus_tag="AC161X_01825"
                     /inference="COORDINATES: protein motif:HMM:NF012531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase central domain-containing
                     protein"
                     /protein_id="HIR4988319.1"
                     /translation="MTASFFDLKHFHDACGVGFIAALDRVPSYRMTRLAVECLERLDH
                     RGARAADGTGDGAGLLTQIPVRLINRELSAHGLEVAPERLGLVMCFLSPTHAAASRSV
                     VTQAIESEGMRVLFWRSVPFDPSMLGDHARDVLPLIEQAIVTGSEEGAAFERSLYLAR
                     KRIERAATPGLSIPSASNRTVVYKGLFTASHISDFYWDLRDPDYETAFAIFHQRYSTN
                     TFPSWENAQPFRALAHNGEINTIQSNRSWMVAREKGATPAVWGDRLQDVFPFLQPDIS
                     DSGSLDNVFELLMQSGRSLTHVKEMLMPAAWENVPDLDPDLAAFYAYHAFLTEPWDGP
                     AAIAATDGTSLLVGMDRNGLRPARWTVTPEVVLVASEAGVCPEEEALALATGQLGPGE
                     LIVFDGETGEVQHSDQVKAALASARPYGDWVSTETLRIQASFDNLADDRFDAAALSRV
                     FGYTAEERRLIIADMARGDNPLGSMGDDTGLAVLSSRPRRLTQFFHEMFAQVTNPPMD
                     PIREKLVMSLRIQLGRRGPLLEDTPQQAHLIELASPILSDAELEAIVRSGDPRFFSHW
                     IATTWPVSAGPEGMEARLDEICDEAAQAVKLGATILVLSDRETDSVRAPIPMLLALGA
                     VHHHLIDEGVRGSVSLVVVSGEPRDPHDLACLVGFGASAVNPYLAIEHARDLAASGAI
                     DVDPVPAQENYRLAMEGGLLKIMSKMGICTLSAYRGSELFEVIGLSEGVCRKAFRNAP
                     RRLRGIGLKELARQALVRHEGYSSGVPESGGFYKHRRGADTHVTSPMVVLAVQKAVRS
                     GDPEDWERYIEMISDREPALVRDLLTFVQREPVPLDEVEPAEQIMRRFTTAAMSLGAL
                     SSEAHEALAEAMNLIGGHSNSGEGG"
BASE COUNT          560 a          858 c          872 g          462 t
ORIGIN      
        1 cccctccctc gcccgagttg gagtgaccac caatcaggtt catcgcctcg gccagagcct
       61 cgtgggcctc ggaagacaac gcgccaagag acatcgcggc ggtggtgaaa cgacgcatga
      121 tttgctcggc aggctccact tcgtcgagcg ggaccggttc cctttgcacg aatgtcagga
      181 gatcacggac cagggcaggt tcccggtcag agatcatctc gatgtatcgc tcccagtcct
      241 ccgggtcgcc agagcgcacg gccttctgca ccgccaggac caccatgggg ctcgtcacgt
      301 gggtatcggc accgcgtcgg tgcttgtaga agccgccgga ctcgggcact ccgctggaat
      361 agccctcatg tcgcaccaat gcctgacgag ccagctcctt gagcccgatc ccgcggagtc
      421 ggcggggagc gttgcggaaa gccttgcgac acactccctc ggagagccca atcacctcga
      481 acaactccga gccgcgatag gcggaaagag tgcagatccc cattttcgac atgatcttga
      541 gcagcccgcc ctccatcgcc aggcgatagt tctcctgagc cggcactgga tccacatcga
      601 tggcccctga cgcggcgagg tcacgggcat gctcgatggc caggtagggg ttgacggcag
      661 aggcgccgaa accgaccagg caggcgaggt catgcgggtc acgtggctca ccggacacca
      721 cgaccaagga aaccgagcca cgcacgcctt cgtcgatgag atggtgatgc accgcaccca
      781 aggcgagaag cattgggatc ggcgcccgaa cggaatccgt ctcacggtcg gagaggacga
      841 ggatggtggc cccgagcttg accgcctgcg cagcctcatc gcagatctcg tcgaggcggg
      901 cctccattcc ctcgggtcca gctgacaccg gccaggtggt cgcgatccaa tgagagaaga
      961 atcgtgggtc gccagagcga acgatcgctt ccaactcggc atccgagagg atcgggctgg
     1021 ccagctcgat gaggtgagcc tgctgaggtg tgtcttcgag gagcgggcct cggcgtccaa
     1081 gctggatccg aagggacatc accagctttt cccgaatcgg gtccatcggc ggattggtga
     1141 cctgggcaaa catctcgtgg aagaactggg tgaggcgacg gggtctgctc gacaggaccg
     1201 ccagccccgt gtcgtcgccc atagatccga gtggattgtc ccctcgtgcc atatccgcga
     1261 tgatcagccg ccgctcctcg gcggtgtatc cgaagacccg gctcaaggcc gcagcatcga
     1321 atcggtcgtc ggccagattg tcgaacgagg cctgaatgcg gagtgtctcg gtgctcaccc
     1381 agtccccgta tggccgggct gaagccaagg cggccttgac ctggtcagag tgctgaacct
     1441 ctccggtctc gccatcgaac acaatcagct ccccgggccc gagctggccg gtggcgagag
     1501 ccaaggcttc ttcctccggg cagaccccgg cctcggaagc gacgaggacg acctcggggg
     1561 tgacggtcca acgggcgggt cgcagcccgt tgcggtccat gcccaccagg agggaagtgc
     1621 catcggtcgc ggcaatggcg gccggcccat cccacggctc ggtgaggaaa gcgtgatacg
     1681 catagaaggc cgccagatcg ggatcgagat caggaacgtt ctcccaggca gccggcatca
     1741 gcatctcctt gacgtgcgtc agcgaccggc cgctctgcat caacagctcg aagacgttgt
     1801 ccaggctccc cgagtccgag atgtcaggct gcaggaaagg aaagacatcc tgcaaccgat
     1861 ccccccacac cgccggagta gcgcccttct ccctcgccac catccacgag cggttggatt
     1921 gaatggtgtt gatctccccg ttatgggcca atgctcggaa cggctgggca ttttcccagg
     1981 aggggaaggt gttggtggag taacgctgat ggaagatggc gaatgccgtc tcgtagtcgg
     2041 ggtctctcag atcccaatag aagtcggaga tgtgagaggc ggtgaacaaa cccttgtaga
     2101 ccacggtgcg attcgaagcc gaagggatcg acaatccggg ggtggcggcc cgttcgattc
     2161 tcttccgagc caggtagagg ctccgctcga aggctgcccc ttcttcggag ccggtcacga
     2221 tcgcctgctc gatcaaggga aggacgtcgc gggcgtgatc gccgagcatc gatggatcga
     2281 acggcaccga tctccaaaac agaacccgca tcccttcgga ctcgatcgcc tgggtaacca
     2341 ccgatcggga ggcagcggca tgggtgggag acaggaagca catcacaagt ccgagacgct
     2401 cgggagcgac ctcgagccca tgggcactca gttcccgatt gatgaggcga acgggaattt
     2461 gagtaaggag gccggcgcca tctccggttc catccgccgc cctcgcacct cgatgatcga
     2521 gtcgttccag gcactccaca gcaaggcggg tcatccgata cgaggggacg cggtcgagag
     2581 cagcgataaa cccgacgcca caggcgtcgt gaaagtgctt cagatcgaag aaagatgccg
     2641 tcattgctcc ctcgaaatgg gggagcgatc aagtttaaac acgtcccagg tcgcaagtcc
     2701 caggtcccaa gcttctggct taggacttga gacctgagac tcaacgcttc ga
//
LOCUS       DBNHXU010000068         4731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4584865, whole genome shotgun sequence.
ACCESSION   DBNHXU010000068 DBNHXU010000000
VERSION     DBNHXU010000068.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4731
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4584865"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="AC161X_01830"
     CDS             complement(<1..462)
                     /locus_tag="AC161X_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988320.1"
                     /translation="MPNRLHVDGSWPDPVTVRRGWARASARPWNDDGPDAAMRLIRGS
                     HEFLAEASRSLESLDIRDIFSPALYPIATRIWARAGYKEHTSLSVMERYLTGNLTEPT
                     YPVQTTDQPDWPSLARVDRAAFTEFWRMSTAGLSEAVRATPRSAVLQIEEEN"
     gene            481..1485
                     /locus_tag="AC161X_01835"
     CDS             481..1485
                     /locus_tag="AC161X_01835"
                     /EC_number="3.5.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF013046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000118 - histone deacetylase complex
                     [Evidence IEA];
                     GO_function: GO:0004407 - histone deacetylase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HIR4988321.1"
                     /translation="MKVVVASHPSSLMHRTTEHHPERPARVGAVSEGITQSELEVIEV
                     ESPEARRSELALVHDPTYIEMIEAFCRMGGGFLDSDTFASRESWVAALTAAGGVTAAI
                     EELEGRTDATGFVIARPPGHHALRSTAMGFCLFNNVAVGAALLRAQGSRVAILDWDVH
                     HGNGTQEMVMADPGILYVSIHQDAFYPFQGDIVDIEREAKGTTVNLPMPAWTGGDLYR
                     RAWAEIVIPVVQAFEADWVLVSAGFDAHVNDHMAELRLVADDYGCMASGLAAIHPANR
                     TVFVLEGGYDLDALRDSTAATLRGTAGNGEFEDPLPSPQPAGHVLDPIFEAVARHWPI
                     "
     gene            1558..2637
                     /locus_tag="AC161X_01840"
     CDS             1558..2637
                     /locus_tag="AC161X_01840"
                     /inference="COORDINATES: protein motif:HMM:TIGR01420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043107 - type IV pilus-dependent motility
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type IV pilus twitching motility protein PilT"
                     /protein_id="HIR4988322.1"
                     /translation="MENKIDELLRQAASQEATDIHLKVGSAPVARINGEIRRLQGFDV
                     LSPEDTQVFADTLFTPRAEADFKNLGFADFAYGRPDLGRFRVSAFRQRGSVSLVLRKV
                     VRESKTFSELGLPNMIEKFAAEKSGLLLVTGPSGSGKTSTMSAIIDWINSNRGVSILT
                     IEDPIEVLHSDKRSVVVQREVGVDTSDYAQAIRSAMRHDTDVIIVSEIEDLETARAAI
                     AASETGHLVISSMRTTDPADTINRIISLFPETEQGITRSQLATQMLGIISQRLLEGQE
                     GTSVLSYEIVNCNERVQEWIIGAKDAGALIDIMKESEFFGMQTFDQSMLKLVVNRVIE
                     LPTALPHSRNVHELRAKAMAAGIAI"
     gene            2708..4126
                     /locus_tag="AC161X_01845"
     CDS             2708..4126
                     /locus_tag="AC161X_01845"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HIR4988323.1"
                     /translation="MIPKRLEFLVSPELPPAQLAPRFNDSGHQLFLVGGSVRDAFLER
                     PVEDYDFATDARPEQIAGILEGWADAIYTVGAAFGTVAAIKDGRTVEVTTFRDEIYRD
                     DSRKPRVTFSDRIEDDLARRDFTVNSIALPLMAMEPVDPYGGLADLAAGRLRTPIDPT
                     VSFSDDPLRMLRLFRFQASLGFEPDPEALAAVGSMAEWLEIVSAERIRDELSKLLTAP
                     APGLALHGVVSSGLAAHFLPELPALAMEQDPHHRHKDVLAHTFAVVDKASPDLTLRLA
                     ALFHDIGKPDTRQFGPDGVSFHHHEVVGARMTRRRLGALRYPKEVIDDVSQLVFLHLR
                     PHTLKMGWTDSAVRRYVRDAGHLLGYLNELVRCDVTTANPRREKEITNRIGELETRIE
                     VLSEQEELAALRAPIDGHQVMEYLAIPPGRMVGDIMDLLLERRIDDGPYSEEAAFGLV
                     REWALERGIADPGPPPSSEEEE"
     gene            4123..>4731
                     /locus_tag="AC161X_01850"
     CDS             4123..>4731
                     /locus_tag="AC161X_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003047107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase domain-containing
                     protein"
                     /protein_id="HIR4988324.1"
                     /translation="MKVVLAYSGGLDTSVILAWLREEHGAEVVTYTADVGQGAEVGEA
                     KVKALATGASEAVVEDLRDRFVTGAVFPALRASAVYEGHYLMGTSLARPIISEGLVRC
                     AREFGADTISHGATGKGNDQVRFELSIAALAPELKVIAPWREWALKGRSDLMDYARER
                     GIAVPVTKEAPYSTDANLLHVSYEGGVLEDPWTAPPDKMFRLT"
BASE COUNT          877 a         1472 c         1482 g          900 t
ORIGIN      
        1 attctcctcc tcgatctgga gcacggcgga ccgtggcgtc gcccgaacgg cttcactcag
       61 gccggcagtg ctcatcctcc agaactcggt gaacgcggcc cgatcgactc tggcaagaga
      121 cggccaatcg ggctggtcgg tggtctgcac cgggtaggtc ggttcggtta agttccccgt
      181 caggtaacgc tccatcacgg agagcgaggt gtgctccttg tatccggcac gggcccagat
      241 gcgggtcgcg atcgggtaca gagccggtga gaagatgtca cggatgtcca gcgattccag
      301 acttcgggag gcttcagcga gaaattcgtg cgatccacgg atcagtctca tcgctgcgtc
      361 ggggccgtcg tcgttccacg gtcgcgcgct ggcccgggcc catccccgtc gaacggtcac
      421 aggatcaggc caggagccat ccacatggag gcggtttggc acggatgaga gggtacgggc
      481 atgaaggtcg tagtcgcatc ccacccgtca tctctcatgc acaggaccac cgagcaccat
      541 cccgagcgac ccgcccgagt gggagcggtg agcgagggga tcacccaaag cgaactcgaa
      601 gtgatcgagg tggaatcccc cgaagctcga cgcagcgaac tggcgctggt tcacgacccg
      661 acctatatag aaatgatcga agccttctgt cggatgggcg gcgggttcct cgacagcgac
      721 accttcgctt cccgggaaag ttgggtggcc gctctcaccg cggccggagg ggtcaccgcg
      781 gcaatcgaag agcttgaagg gcgaaccgat gccacgggat tcgtcattgc ccgcccacct
      841 ggccatcacg cactgcgcag cacggcgatg gggttctgcc tcttcaacaa cgtggcggta
      901 ggagctgccc tgctccgcgc gcagggcagc cgggtggcaa tcctggattg ggatgtccat
      961 cacggcaatg ggacccagga gatggtgatg gctgatcccg gaatcctcta tgtgtccatc
     1021 caccaagatg ccttctaccc cttccagggc gacatcgtcg acatcgagcg cgaagcaaaa
     1081 gggacgacgg tcaacctgcc catgccggcc tggaccgggg gcgacctcta tcggagggcc
     1141 tgggcggaga tcgtgatccc cgtcgtccag gcattcgagg ccgattgggt gctggtcagc
     1201 gccggatttg acgcccatgt caacgatcac atggccgaat tgcgcctggt agccgatgac
     1261 tacgggtgca tggcttccgg actggccgca attcaccccg cgaaccgcac cgtcttcgtc
     1321 ctcgaaggcg gttatgacct ggacgccctc cgtgattcga cagctgccac gctgcgcgga
     1381 actgccggca atggtgagtt cgaagacccg ttgccctccc cacagcccgc gggccatgtc
     1441 ctcgatccca ttttcgaggc cgtggccagg cactggccga tctgaccagc aatttccttc
     1501 aatttcggct gtctacggcc gataaacagc tgctgggaag acattccggg aagggacatg
     1561 gaaaacaaga tcgatgaact cctgcgtcag gctgcgtctc aagaggcgac tgacatccat
     1621 ctcaaggttg gttcggctcc agttgctcgc atcaacggtg agatccgcag actccaaggc
     1681 tttgacgtac tgtctcctga ggacacccag gtcttcgccg acactctttt cacaccccgg
     1741 gccgaggccg acttcaagaa cctcgggttc gccgacttcg cgtatggacg gccggaccta
     1801 ggtcgtttca gggtttccgc gttccggcag cgtggctcgg tgtctctcgt acttcggaag
     1861 gtggttcgcg agtcgaagac attctcagag ctcggcctgc ccaacatgat tgagaagttc
     1921 gctgccgaaa aatcaggtct cctgttggtc accgggcctt cgggctcagg gaagacctcg
     1981 acgatgtcgg cgatcatcga ctggatcaat tccaatcggg gagtctcgat cctcaccatc
     2041 gaggatccga tcgaggtcct ccacagcgac aagcggagtg ttgtggtcca acgtgaggta
     2101 ggagtcgata cctccgatta cgcccaagcg atccggagtg ccatgcggca cgacaccgac
     2161 gtgatcatcg tgtcggagat cgaggatctc gaaaccgccc gggccgccat cgcggcttcg
     2221 gagacaggtc acctggtgat ctcctcgatg cgcaccaccg atcccgccga cacaatcaat
     2281 cgcatcattt ccctcttccc ggagaccgag caggggatta cccgatccca gctggccaca
     2341 cagatgctgg ggatcatcag ccagcgcctc ctcgaaggtc aggaaggcac ctccgtgctg
     2401 tcctatgaga tcgtgaactg caacgagcgg gttcaggaat ggatcatcgg agccaaagac
     2461 gccggtgcgc tgatcgacat catgaaggag agcgagttct tcggaatgca gacgttcgac
     2521 cagtcgatgt tgaagctggt cgtcaatcgt gtcatcgagc ttccgactgc cctgccccat
     2581 tcccgcaacg tccatgagct tcgagccaag gcgatggccg ccggaatagc catttaggcg
     2641 tattacgtag tacgtattac gtaggacggg ggacccgaag gggtcccctc cctttaacct
     2701 cagcggtgtg attccgaagc gtctcgagtt tctcgtctct ccagagcttc cgcccgcgca
     2761 gctggccccg cgtttcaatg attcaggcca tcagctgttt ctggtcggag gctcggtccg
     2821 tgacgccttt ctcgagcgcc cggttgagga ctacgacttt gcgaccgatg cccgccccga
     2881 gcagatagca gggatcctcg aggggtgggc ggacgcgatc tacaccgtgg gtgccgcatt
     2941 cgggactgtc gcggcgatca aggatgggcg gactgtggag gtcaccacct ttcgcgatga
     3001 gatctatcgc gacgactcac gcaagccccg cgtcaccttc tcggaccgaa tcgaggatga
     3061 tctcgccagg cgcgacttca ccgtcaattc catcgccctt cccctgatgg ccatggagcc
     3121 cgtggatccc tacgggggcc tggccgacct ggcggcgggg cggctgcgga ctccgatcga
     3181 tccgacggtt tcgttctcgg acgacccatt gcggatgttg cggctgtttc ggttccaggc
     3241 cagcctcggg ttcgagccgg atccggaagc gctggccgca gttggctcga tggcggagtg
     3301 gctcgagatc gtctccgcag agcgaatcag ggatgagctc tccaagctgt tgaccgctcc
     3361 cgcgcccggg ctcgcactcc atggcgtcgt cagctcgggc ctggccgccc acttcctccc
     3421 tgagctgccg gcgctggcca tggaacagga cccccaccat cgtcacaaag acgtgctggc
     3481 tcacactttc gcggtcgttg acaaggcgtc tcctgatctg acgttgaggc tggccgcgct
     3541 tttccacgac ataggaaagc cggatacccg gcagtttgga ccggatgggg tgtcgtttca
     3601 ccaccatgag gtggttggag cacggatgac ccgtcgtcga ctcggggcat tgcgctatcc
     3661 gaaggaggtc atcgacgacg tctcgcagct ggttttcctc caccttcggc cccacaccct
     3721 caagatggga tggaccgact cggcagttcg ccgctacgtg cgggatgccg gtcatctgct
     3781 cggctatctg aatgagctgg tccgctgcga cgtcacaacg gccaatccga gaagagagaa
     3841 ggagatcacc aaccggatcg gcgagctcga gacccggatc gaggttctct ccgagcagga
     3901 ggaattggct gcacttcgag ctccgatcga cggccaccag gtgatggagt acctcgcgat
     3961 tccgccaggc aggatggttg gtgacatcat ggacttgctg ctcgaacgtc gcatcgatga
     4021 cggtccgtac tcagaagagg cggccttcgg gctggtgcgg gaatgggcgc tggaacgcgg
     4081 cattgccgac cccgggccgc ccccgtcgtc agaggaagag gaatgaaggt cgtactcgca
     4141 tattcgggag gtctcgatac ctcggtgatc ctggcctggt tgcgcgagga gcacggtgcc
     4201 gaagtcgtga cctacaccgc cgatgtgggt cagggggcgg aggtgggcga ggccaaggtg
     4261 aaggcccttg ctactggggc gtcggaggcc gtggtcgaag acctccgcga ccgcttcgtg
     4321 accggcgccg tctttccggc cctgcgggcg tctgccgtct acgagggcca ctacctgatg
     4381 ggcacttctc tcgcccgccc catcatcagc gagggcctgg tccgttgtgc ccgtgagttc
     4441 ggggccgaca cgatttcgca tggcgccacc ggcaagggaa acgaccaggt ccggttcgag
     4501 ctctcgatcg ccgccctcgc tcctgagctc aaagtcatcg caccatggcg ggagtgggca
     4561 ctcaagggcc ggtccgacct gatggattac gcacgcgagc ggggaatcgc ggtaccggtg
     4621 accaaggaag ccccctactc gacggatgcc aaccttctcc atgtctcgta cgagggcggt
     4681 gtcctcgagg atccgtggac cgcaccacct gacaagatgt tccggctcac g
//
LOCUS       DBNHXU010000069         4929 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4188057, whole genome shotgun sequence.
ACCESSION   DBNHXU010000069 DBNHXU010000000
VERSION     DBNHXU010000069.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4929)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4929)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4929
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4188057"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..1314
                     /locus_tag="AC161X_01855"
     CDS             22..1314
                     /locus_tag="AC161X_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase family protein"
                     /protein_id="HIR4988325.1"
                     /translation="MPAAIPDRYRLEMRLGRDGDLEEWLATDTSLERPVLVRSLGPES
                     TTERRSQFVASVGDAAKTTHPHLARVFAVEVVAGGAYSVSEWTGGATAQDRVDAAQTF
                     EFEEFLPNAAGLAGALAALHQVGGVHGGLDLSAIYYSVAHAAKLGAFGRQPRTDANGD
                     VRSLSAALETALTGSSPGGPPPSERIDGIPRTLDAVLRSGQTGRLSSADLEKALLASP
                     TPRMPRPEPHSTSRRLLLAAGILVVLAVALVAIGLLFTGGSPVVPVSPTATSPGAVAS
                     TTHPPTTTLPPAAPVEVIEATSFDPFGEGGESDTLIPNLFDGATSSSWRTERYQDPLP
                     LLKPGVGLRFAIDGEPRRLELIELSPETVFEIYWSPEADVDLSEWERIAGAKSPPGTT
                     SVDLPARSNGYWLVWMVDLPQQSEGTYYSSMAEVRFLG"
     gene            1311..1859
                     /locus_tag="AC161X_01860"
     CDS             1311..1859
                     /locus_tag="AC161X_01860"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4988326.1"
                     /translation="MTSPGTETDLLARVISGDREAFDSIMRAQEDRVFSVCLRILGDR
                     ENALDATQDTFLTVFRKAAQFKGDSALGTWIYRIAVNTCYDQIRRTGRRPTETLPDHL
                     EPPDASAEDAINSASLRPEIELALASLPPDFRAAVVLSDLEGMSLPDIATVLGIPVGT
                     VKSRVFRGRRLLAELLGNQTTA"
     gene            1879..2649
                     /locus_tag="AC161X_01865"
     CDS             1879..2649
                     /locus_tag="AC161X_01865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988327.1"
                     /translation="MHDHDLDLIASLADGSLEEQAEARALVDSCEECRHEYDTQVSVL
                     GALSGLEPARLTELEKAGLHRDLWTELRSEAATKTRAIPWWYRLSYAAAGLFVMVGLV
                     AVLGQGVFGGGDSAESFSEVSSGLDGGASAESVTPLYSPQSDESPGAEPTTTAGATDT
                     TVSTPPSVTTLESAATDFKTVADDTRQGQLPATDRALAIERAECLGAAGLENYEFEGT
                     VELERTYLVAVPAGVDLDSETPVTFVDADNCEVVHVEE"
     gene            2683..2949
                     /locus_tag="AC161X_01870"
     CDS             2683..2949
                     /locus_tag="AC161X_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988328.1"
                     /translation="MEDYAAKFAELLEQIATRIRSVTVDRVAKGIRLTGLGILAVTLA
                     FTAVLFLLFAIFGALEIPLTTAGALALIGVLLIGVGTYLWIKRT"
     gene            2957..3883
                     /gene="trxB"
                     /locus_tag="AC161X_01875"
     CDS             2957..3883
                     /gene="trxB"
                     /locus_tag="AC161X_01875"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006928360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADPH) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HIR4988329.1"
                     /translation="MSHEKLIIIGSGPAGLTAALYASRADLGPLVFEGSAAGGQLMIT
                     TDVENYPGFPDGILGPELMDQFRKQAERFGARLRQIDVTEVDFSSHPYRLKVGADEFT
                     ADAVIIATGASAKWLGIPGEEQLTGRGVSACATCDGFFFRDQELIVVGGGDTAMEEAL
                     FLTKFASKVTIAHRRDEFRASKIMAARVMEHPKIDVLWNTVVTEIHGDTTVSSVTLED
                     TVSGERREMAANGVFVAIGHKPNTEIFAGQLDLDANGYLLTVGDGSTRTKLEGVFAAG
                     DVADHIYRQAVTAAGTGCMAAIDAERWLADQE"
     gene            3936..4253
                     /gene="trxA"
                     /locus_tag="AC161X_01880"
     CDS             3936..4253
                     /gene="trxA"
                     /locus_tag="AC161X_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007032253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HIR4988330.1"
                     /translation="MAEIVTLSDGTFNEAISGDKPILVDFWAEWCGPCRIIEPVLKEL
                     ATENDNITFGKLNIDHHPMPAANNDVMSIPTLILFKDGVEKKRIVGAAPKHRLLQELS
                     EFL"
     gene            4345..>4929
                     /locus_tag="AC161X_01885"
     CDS             4345..>4929
                     /locus_tag="AC161X_01885"
                     /inference="COORDINATES: protein motif:HMM:NF013625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HIR4988331.1"
                     /translation="MRLYRLGDEGAPVRDIQDRLSSLGYESNSDPRGVFGESTVVAVK
                     DFQKAKGLVVDGIVGPNTWRSLYEAGYRLGDRLLFFRRPMFRGEDVTELQSRLNSMGF
                     DAGKVDGIFGPKTEAAVVEFQHNRNLAEDGKAGPEVVTEVHLVTRGEMKESRHAVRER
                     EWLRRLPTTVAGARIYVDAGCRDPHEATMAWEAAS"
BASE COUNT          916 a         1587 c         1547 g          879 t
ORIGIN      
        1 cactgatgct ggactgagta gatgccggcc gcaataccag accgctaccg cctcgagatg
       61 cgtctcgggc gggatggcga tctcgaggaa tggctggcca ccgacacctc gttggagcgc
      121 cccgtgctcg tcaggtcact ggggccggaa agcaccactg agcggcgcag ccagttcgtg
      181 gccagcgtcg gcgacgctgc caagaccacc cacccccatc ttgcccgggt gttcgcggtg
      241 gaggtggtgg ccggcggtgc ctactcggtt tcggagtgga ccggaggggc aactgcacag
      301 gatcgcgttg acgcagccca gaccttcgag ttcgaggagt tccttccaaa cgccgcaggc
      361 ttggccggtg cgctggcagc cttgcatcag gtgggcggag tccacggcgg cctggatctg
      421 tcggccatct actactccgt cgcccatgct gccaagctcg gtgccttcgg ccgccagcca
      481 cgaaccgacg ccaacggtga cgtgcgctcg ctgtcagcgg ccctggagac tgccctgacc
      541 ggatcgtcgc ctggcggacc acctccgtcg gagagaatcg acgggatacc gcggacgctc
      601 gatgccgttc tcaggtccgg tcagacgggc cggctcagct cagctgacct cgagaaggcc
      661 ctcctcgcct ccccgactcc ccggatgcca cgccccgagc cccactcgac ctcccgtcgg
      721 ctcctgctgg cagctgggat cctggtggtc ctggccgtgg ctctggtcgc aatcgggctc
      781 ctctttaccg gcgggtctcc cgtggtgccg gtgtcgccaa ccgcgacctc gcctggcgcg
      841 gtcgccagca ccacccaccc cccgacgacg acactgcccc ccgccgctcc tgtcgaagtc
      901 atcgaagcga cctccttcga cccgtttggt gagggcggtg agagcgacac cctgatcccc
      961 aatctgttcg atggcgccac ttcgtcttcc tggcgaaccg agcgctacca ggacccgctg
     1021 ccccttctca agcccggtgt ggggctccgc ttcgccatcg atggcgagcc gcgtcgcctc
     1081 gagctgatcg agctcagccc cgagactgtc ttcgagatct actggtcgcc cgaggccgac
     1141 gtcgatctgt ccgaatggga gcggatcgcc ggagcaaaat cgccaccggg gaccacttcg
     1201 gtggacctgc cggcacggtc caacggttac tggctggtct ggatggtgga cctgccacag
     1261 caatccgaag gcacctatta ctcgtcaatg gcagaagtac gcttcctcgg gtgacatcgc
     1321 cagggaccga gaccgacctg ctggcacgtg tcatctcagg cgaccgggaa gccttcgact
     1381 cgatcatgcg agcccaggag gacagggtgt tctccgtttg cctccggatc cttggagacc
     1441 gggaaaacgc cctcgacgcc acccaggaca cattcctcac cgtcttcagg aaagcggccc
     1501 agttcaaggg ggattcggcg ttggggacct ggatctaccg gatcgccgtg aacacctgtt
     1561 acgaccagat ccggagaacc gggcgacggc cgacggagac cctgcccgac catctcgagc
     1621 cacccgatgc gtcggccgaa gacgcgatca actcggcgag tttgcgaccc gagatcgaac
     1681 tggcgcttgc ttcgttgccg ccagatttca gggctgcggt ggtgctgtcg gacctcgagg
     1741 ggatgtccct tccggacata gccacggttc ttggtattcc ggtggggacg gtgaaatcgc
     1801 gggtcttccg cggacggcgg ctgctggcag aactgctggg gaaccaaacg acggcatgag
     1861 accatcaaag agttacggat gcatgaccac gatctcgacc tgatcgcctc cctggccgac
     1921 ggctccctcg aagagcaggc tgaggcgcgc gctctggtgg acagctgcga ggaatgccgt
     1981 catgagtacg acacccaggt ttcggttctc ggggcactct cggggttgga gccggcccga
     2041 ctgaccgaac tggagaaggc aggcctgcac cgggacctgt ggaccgagct gcgcagtgaa
     2101 gccgcaacca agacgcgggc cattccctgg tggtaccggc tgtcgtatgc cgctgcgggc
     2161 cttttcgtga tggttgggct ggtggccgtt ctcggacaag gagtattcgg tggaggcgac
     2221 tccgctgaat cgttctctga ggtctcctcg ggactcgatg gcggagcttc cgcggagtcc
     2281 gtcacgccgc tctactcccc tcagtcggat gagagtcctg gagcggagcc aaccaccacc
     2341 gctggggcca ctgacaccac cgtcagcact cccccgtccg tcaccacctt ggagagtgcg
     2401 gcaaccgatt tcaagaccgt ggctgacgac acccgtcagg ggcaattacc cgccaccgat
     2461 cgggctcttg ccatcgagag ggccgaatgc ctgggtgccg ccggtctcga gaactacgag
     2521 ttcgaaggga ccgtcgagct ggagcgcacc tatttggttg cggtgcccgc cggagtggac
     2581 ctcgactcgg aaaccccggt taccttcgtc gacgccgata actgtgaggt tgtccacgtc
     2641 gaggagtgat tctcggcagt cactaactaa cctcgcctcc taatggagga ctacgcagca
     2701 aaattcgccg agctcctgga gcagatcgcg accaggatca gatcggtcac cgtcgatcgg
     2761 gtggcaaaag ggatccgcct caccggcttg ggaatcctgg ccgtcaccct tgcgttcacc
     2821 gcggtgctgt tcctgctgtt cgccatcttc ggggcgctcg agatcccatt gacgactgca
     2881 ggggcgctgg cactgatcgg tgtcctgctg ataggcgttg gcacctatct ctggatcaag
     2941 cgaacctgaa agaactatga gtcacgagaa actgatcatc atcggatccg gtccggcagg
     3001 tttgaccgcg gccctttacg catcccgcgc cgacctcggc cctctggtgt tcgaagggtc
     3061 ggccgcgggt ggtcaattga tgatcaccac cgacgtcgag aactacccgg gcttccccga
     3121 cgggatcctc ggtcccgagc tcatggacca gttccgcaag caagccgagc gattcggggc
     3181 ccgtctgcgc cagatcgacg tcaccgaggt cgatttcagc tcacacccgt accgtttgaa
     3241 ggtcggagcg gatgagttca ccgccgatgc ggtgatcatc gccacgggag cctcggccaa
     3301 gtggctggga atccccggcg aggagcagct caccgggcgc ggtgtctccg cctgcgccac
     3361 ctgtgacggg ttcttcttca gggaccagga gttgatcgtg gtcggaggtg gcgacaccgc
     3421 aatggaggaa gccctgttcc tcaccaagtt cgcctcaaag gtgaccatcg cccatcgtcg
     3481 cgacgagttc cgtgcctcga agatcatggc ggcccgagtc atggagcatc ccaagatcga
     3541 cgtgctctgg aacaccgtgg tcaccgagat ccacggcgac accactgtct cgtcggtgac
     3601 cctggaagac accgtcagcg gggagcggcg ggaaatggcg gccaatggcg tcttcgtagc
     3661 catcggccac aagccgaaca ccgagatctt cgccggacaa ctcgacctcg acgcaaacgg
     3721 gtacctgctc acggttggtg atgggagcac ccgcaccaag ttggagggag tgtttgctgc
     3781 aggtgatgtc gccgatcaca tctatcgtca ggccgtcacc gcggccggga ccggatgcat
     3841 ggctgcaatc gacgccgaac ggtggctggc agaccaggaa tgaactcagg gcggccatcg
     3901 ttcaccctga tagctgacaa ggagtaggac cagacatggc cgagatcgtg accctgagcg
     3961 acggcacctt caatgaggcg atcagcggcg acaagccgat actcgtcgac ttctgggcgg
     4021 aatggtgcgg gccctgtcga atcatcgagc ctgtactgaa agagttggcg accgagaacg
     4081 acaacatcac tttcggcaag ctgaacatcg accatcatcc gatgccggca gccaacaacg
     4141 acgtgatgag cattcccacc ttgatcctct tcaaggacgg cgtcgagaag aagcggatag
     4201 tcggcgccgc tcccaagcac cggttgctgc aggaactctc cgagttcctc tagacgggca
     4261 tcgctcttct cgaggcgcaa tacgcagtac gaacacgcgt tcgctggatc acgctaaacc
     4321 cgtctctcgt gccagcatgt gcctatgcgg ctctaccgcc tcggtgacga gggcgccccg
     4381 gttcgggaca tccaggatcg tctcagctca ctcggatacg aatccaactc tgatccacgt
     4441 ggtgtattcg gggagagcac tgtcgtcgcg gtcaaagact tccagaaggc caaaggcctg
     4501 gttgtcgacg gaatagtcgg ccccaacacc tggaggtccc tctacgaggc cggctaccgc
     4561 ctgggggacc ggttgctctt cttccgacga ccgatgtttc gcggcgagga cgtcaccgag
     4621 ctccaatcac gcctcaactc catgggattc gacgcgggga aagtcgacgg gatcttcggc
     4681 cccaagaccg aagcggctgt ggtggagttt caacacaacc gcaacctcgc cgaagacggg
     4741 aaagcgggac cagaggtggt gaccgaagtc catctcgtga cccgtggcga gatgaaagag
     4801 agccgacatg cggttcgaga aagggagtgg ctccgtcggc tgccgacgac cgtggcaggc
     4861 gccaggatct atgtcgatgc cggttgccgg gacccccacg aggcaaccat ggcctgggag
     4921 gcggcctcg
//
LOCUS       DBNHXU010000070         2518 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1212568, whole genome shotgun sequence.
ACCESSION   DBNHXU010000070 DBNHXU010000000
VERSION     DBNHXU010000070.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1212568"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="AC161X_01890"
     CDS             complement(<1..484)
                     /locus_tag="AC161X_01890"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR4988332.1"
                     /translation="MTIASMAQTTNVPSVGTWQIDRSHSDLRVTARHLMVAKVRGTFT
                     EFDGTIVVAEDPTKSSVEFEAKAASVTTGTPDRDNHLRSPDFLDAEQYPTIKFVSTGL
                     VSRGKDWDLTGDLTIRDITRPVSFHMKYEGSATDPYGNSKAAFSAVGEIERKDWGLTW
                     N"
     gene            597..1079
                     /locus_tag="AC161X_01895"
     CDS             597..1079
                     /locus_tag="AC161X_01895"
                     /inference="COORDINATES: protein motif:HMM:NF024212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR4988333.1"
                     /translation="MTTATSRALVEQEQATWQAYFDATRLLFEELDRRVSEDAGISLA
                     DFGVLARLADADEWGMRMSDLADSTVFSKSRISHAVDRLETLGLVERRACPTDRRGSY
                     ASLTEAGRDKLTEAMPGHSETVRRHLFEQLSTDEVDGFRASMTAVKKSLQEADPASVA
                     "
     gene            1084..1461
                     /gene="acpS"
                     /locus_tag="AC161X_01900"
     CDS             1084..1461
                     /gene="acpS"
                     /locus_tag="AC161X_01900"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011688356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HIR4988334.1"
                     /translation="MRILGLGVDLADIDRVESVLSRYPRFAERCFTEHEREYAFRFSN
                     PARRLAARFAGKEAVMKSLGTGWRRVRWQDIEITGGGKPTVRMTGTAARRADQLGVTE
                     VLVTITHTNTSALVMAVAVSEQH"
     gene            1512..>2518
                     /locus_tag="AC161X_01905"
     CDS             1512..>2518
                     /locus_tag="AC161X_01905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR4988335.1"
                     /translation="MTATDRRRGAEVFTERLGVGLEGHGWVVDMASLTRSGDARRVPI
                     ESLTDVDSSRPGRMNRRIGRALNQRIKAFQPDLVLANGGSTLRYAALATLRTKPPLVY
                     IAIGEPDYWIRSGPARIANRWMLRRATRVFAVCEATARQLRVLEPSLAGRVDVTYTGV
                     PDEMFRSRSGRDIGPLRVVMVGSLSEEKDPLLALRSVAAVPDAVIRFVGSGPLTDQVR
                     SEAERLGVDERVEMVGAVDDVGTQLEWANVLLLTSRTEGLPAAILEAGASSVPAVAVD
                     VGGVREAIVNEDTGIVVQGREAAALTTVLARLAADPDLVTRMGEAARAHVLDRFAMDR
                     I"
BASE COUNT          444 a          729 c          839 g          506 t
ORIGIN      
        1 cattccaggt caggccccag tccttgcgct cgatctctcc gactgcggag aaggcagcct
       61 tggagttgcc ataggggtcg gtggcacttc cctcgtattt catgtgaaag ctcaccggtc
      121 gggtgatgtc acgaatggtc aggtcaccgg tgaggtccca gtccttgcca cgggagacga
      181 ggccggtcga gacgaatttg atcgtcgggt actgctcagc gtcgaggaag tcgggggagc
      241 gcaggtggtt gtcccggtcg ggggttcccg ttgtcaccga agccgctttg gcttcgaact
      301 cgacgcttga cttggtcggg tcttcggcca ccacgatggt tccgtcgaac tccgtgaatg
      361 tgccccgcac tttggccacc atcaggtgtc gcgccgtaac gcgcagatca ctgtgggacc
      421 tgtcgatctg ccaggtgccg accgagggaa cgttggtcgt ctgggccatg cttgcgattg
      481 tcatcgaact tcctcctgtg tgtgagaatt gttgaactgt caaccacacc gtatcgctaa
      541 atgtttgaac tgtcaactat tcgagtttca accaacgtct cgctatgatg tcgtgcatga
      601 ccaccgcaac gtctcgagct ctcgtcgagc aggaacaggc gacctggcag gcgtatttcg
      661 acgccaccag gctgctcttc gaggaactcg accgtcgggt gagtgaggac gccggaatct
      721 cgctggccga cttcggcgtg ctggccaggc tggctgacgc cgacgagtgg gggatgcgaa
      781 tgagcgatct cgccgattcg acggtcttct ccaagagtcg aatatcacac gccgtggacc
      841 gcctcgagac cctcggactg gtcgagaggc gtgcctgtcc cactgatcga cggggcagct
      901 acgcatcctt gaccgaggcc ggtcgtgaca aactgaccga ggcgatgccc gggcattcag
      961 aaacggtccg gcgtcatctg ttcgagcagt tgagcactga cgaggtcgac ggcttccggg
     1021 cttcgatgac agcggtcaag aagtcgctgc aggaggccga tcctgcgtcg gtagcctgac
     1081 gacgtgcgca tcctcggttt gggagttgat ctcgccgaca tcgatcgcgt cgaaagcgtg
     1141 ctgtcgcgat atccgagatt cgccgagcga tgcttcaccg aacatgagcg cgagtacgca
     1201 tttcgcttct ccaatccggc acgtcgcctc gccgcccgat ttgcaggcaa ggaggcggtc
     1261 atgaagtcgc tgggtaccgg gtggcgtcgt gttcgctggc aggacatcga aatcaccggt
     1321 ggcggcaagc cgacagtccg gatgacaggc accgcggcaa gacgcgccga tcaactcgga
     1381 gtcacagaag tcctcgtgac catcacccac accaacactt cggccttggt catggctgtc
     1441 gccgtgtccg agcagcactg agcgtgtcga ctccgcaacg gggcgaaggg gatgggccgc
     1501 gcatactgat cgtgaccgcc accgatcggc gaaggggggc cgaggtgttc accgaacgtc
     1561 tcggagtggg gctcgaaggc cacggatggg tggtcgacat ggcaagtctg accagatcgg
     1621 gtgatgcacg ccgagttccc atcgagtcgc tcacggatgt tgactcgtcc cgcccagggc
     1681 gaatgaaccg gcggattggc cgggccctga accagcggat caaagccttc caaccggatc
     1741 tggtgttggc caatgggggc tcgactctgc gttatgcggc ccttgccact ttgaggacga
     1801 agcctccgtt ggtctacatc gccatcggcg agcccgacta ttggatacgt tcgggtccgg
     1861 ctcggatcgc caatcggtgg atgctgcgac gagccaccag ggtgtttgcc gtctgcgagg
     1921 cgaccgcccg acaactacgg gtcttggagc cctctcttgc gggcagggtc gatgtcacct
     1981 acaccggggt tcccgatgag atgttccggt cgcggtccgg tcgcgacatc gggccgttgc
     2041 gggtggtgat ggtggggagt ctctcagaag agaaggatcc actactcgcc ctgcggagtg
     2101 tcgccgcagt tcccgatgct gtgatcaggt tcgtggggtc cgggccgctg acggatcagg
     2161 tgcgtagtga ggcggagagg ttgggagtgg acgaacgggt ggaaatggtc ggtgctgtcg
     2221 atgatgtcgg cacgcaattg gagtgggcga atgtgttgct cttgacctcg cgcaccgagg
     2281 gactgccggc cgcgattctc gaagccggcg cctcgtcggt gcccgccgtg gccgttgacg
     2341 tcggtggtgt ccgtgaggcg attgtgaacg aagacaccgg aatcgtcgtc cagggaagag
     2401 aagctgctgc cctgaccacg gtgttggctc gtctcgctgc cgacccggat ctggtgaccc
     2461 gcatgggcga ggcagcccgt gctcacgtcc tggatcgatt cgcgatggac cgcatcat
//
LOCUS       DBNHXU010000071         5395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1015332, whole genome shotgun sequence.
ACCESSION   DBNHXU010000071 DBNHXU010000000
VERSION     DBNHXU010000071.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1015332"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..424)
                     /locus_tag="AC161X_01910"
     CDS             complement(<1..424)
                     /locus_tag="AC161X_01910"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA glycosylase AlkZ-like family protein"
                     /protein_id="HIR4988336.1"
                     /translation="MTEVLSTRALNRATLQRQFLLERVQRGVPETIEHLVGMQAQNPL
                     DPYYGLWARLEPFQPETLSALIENRSAVRTALLRATIHLVTANDCLGLDATVRPVSRR
                     TFRSTSFSRDTAGMDIEQLLALGRQLLEAEPMGRAQLGK"
     gene            835..1236
                     /locus_tag="AC161X_01915"
     CDS             835..1236
                     /locus_tag="AC161X_01915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988337.1"
                     /translation="MPESIKIGPIEAVRKFVEGFNNGDVELAQAACAHQISIIDDFPP
                     YGWQGGGAMTGWFRDLARMSTEHGMSDPWVTLREPLQVEMTDRKAYMVVPIDVGWLQN
                     GARAERTGFMTIVLWEEAEGWRISACAWTWN"
     gene            complement(1336..1629)
                     /locus_tag="AC161X_01920"
     CDS             complement(1336..1629)
                     /locus_tag="AC161X_01920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988338.1"
                     /translation="MWILFSVPCPTPDDQTARDLYQKRVDRITDEMRASAVEHGCRFH
                     RAWYASDESAFYAVANWESREGASAFFEQWDIQDEPGEVAVVLEGDIGLVPIP"
     gene            1688..2836
                     /locus_tag="AC161X_01925"
     CDS             1688..2836
                     /locus_tag="AC161X_01925"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR4988339.1"
                     /translation="MRIGILTGGGDVPGLNACIKAVVNRVVSEGHQVVGVRRGWGGLA
                     ETNPDDPKSGEANLVPLDRMAVRTIDRSGGTFLHSSRTNPAKVRTAELPDFLAGDFSG
                     DGPHDITPHVLRVLETHKIDVLIPIGGDDTLSYGLRLHEEGVPVIAIPKTMDNDVHGT
                     DYCIGFSTSVTRGVQFIHSLRTAAGSHERIAVVELFGRYSGETSLITAYLSGVDRAII
                     SEVPFEIDKLAAFLQEDQAANPSNYAMVTISEGARLIEGDIHLTGEEDAYGHRKLGGI
                     GATTGELLKERTGEGILYQQIGYLMRSGAPDSLDLMVATNYAVMAADLAMEGAHGRMV
                     ALRSGTYTDVPIGVTREGVKRVDVDELYDVDAYQPKVRHVLGKPMFLY"
     gene            complement(2841..3350)
                     /locus_tag="AC161X_01930"
     CDS             complement(2841..3350)
                     /locus_tag="AC161X_01930"
                     /inference="COORDINATES: protein motif:HMM:NF014862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR4988340.1"
                     /translation="MFERFGKEARGIVTTAVAEAEARDDSRVGTEHLLIAVAGVSFPF
                     QRSLLGPLDVGSEEIRRQLDRLDSESLAAVGVDLELFDPALRALDGAVPRHARRHLPF
                     THGAKAVLTDTLRETIKQGGRQIGTEHVLLALLSLPAADPLGRVFANMGADPQEIRTD
                     IEEALRRAS"
     gene            complement(3353..3556)
                     /locus_tag="AC161X_01935"
     CDS             complement(3353..3556)
                     /locus_tag="AC161X_01935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005252026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4988341.1"
                     /translation="MDLNKTLLAAEGADPALGLRAVVTLRRLADRLEEIHVASARRQG
                     WTWQQIGDALGVSRQAIHKKSGR"
     gene            complement(3704..4750)
                     /locus_tag="AC161X_01940"
     CDS             complement(3704..4750)
                     /locus_tag="AC161X_01940"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012189108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR4988342.1"
                     /translation="MGDIIPIRDDEGFDVDRLADWLETIPDLPRGAPLVGQFAGGKAN
                     LTYLLTFPDGTELVLRRPPLGPVAPGAHDMAREYRVLSSLWSVFPKAPRALAYCDDVS
                     VLGSPFFMMDRRYGVVVRDTIPPLFGGGHDPASNRKLSEVVVDTLAEFHGVDPVLAGL
                     GDLGHPEDFLARQVDGWTERWNQAKDEDSPVAEEVSGWLVANLPESTEVTLLHNDWRL
                     DNLAVAPDDPGKCVAVYDWDMATRGDPLADLGTLMASWYDPGEERSALNPMPTDAPGW
                     ISRGSAVARYGERSGRALDSIDWYIVFGTWKLGVVLQQIYIRWVRGQTKDLRFSVLGE
                     GARTLFRVAAARRG"
     gene            4816..>5395
                     /locus_tag="AC161X_01945"
     CDS             4816..>5395
                     /locus_tag="AC161X_01945"
                     /inference="COORDINATES: protein motif:HMM:NF017745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF885 family protein"
                     /protein_id="HIR4988343.1"
                     /translation="MTTPFEISDRFTEQWSDLTPIDATIQGVEGRDHLWDDFSPEGEA
                     ARADLARSTRGELVEHLNHPDPKQAFAAKVLAAWMMKKVTVFERGKWKSDLNHIYSPF
                     QRARDTFDVMPKQGEEAWSNIATRLETFGQVTEGYRAALQVGLDVGVTAAQRQVRTLI
                     DQARAVAAEDSRFAGYPTEASSSGGDPTRVANA"
BASE COUNT         1023 a         1638 c         1764 g          970 t
ORIGIN      
        1 gcttgcccag ctgggcccga cccatgggtt ctgcttcaag cagctggcgt ccgagagcga
       61 gcagctgctc gatgtccatt ccggccgtat ccctggagaa tgaggtcgag cgaaaagttc
      121 gtcgggaaac cggtctcacc gtggcgtcga ggccgaggca gtcattggcg gtgacgagat
      181 ggatggtggc ccgaaggagg gcggttcgaa ccgccgagcg gttctcgatc agggcggaga
      241 gcgtctccgg ttggaagggc tcgagtcggg cccacagtcc gtagtagggg tcgagcgggt
      301 tttgagcttg catccccacc aggtgctcga tggtttccgg tacacctctc tgaaccctct
      361 ccagcaggaa ctggcgttgc agggttgccc gattcaaggc ccgggtgctg agaacctcgg
      421 tcaccctcag atgttggcag ggtcgacggt gtcgatgatt gggcgagcgt gggtgcggtg
      481 gcccctaggt tcggacgagt tctcgcagcc gcggatcgag ctcgggtcac ggtggtgggt
      541 agggggtgcc gtcgggcttt tggaaggtca gctggcccgc ggggtcacct tcgatcgacc
      601 agccgtgttc gtggaggaat cggtggtgga acccgccaga ggaggaccag gttgtcggct
      661 atgcatttcg ctagggtccg gggtgatctt caaggacagg aagtggtggt gcctggtggc
      721 gtggggcctg tcggtcctcg ccttcttcca ctggataatc aatccacaga ccggtgtcat
      781 gggcatcatt ctctatggtc gagcgatgag cgcccacgga ggggaggccg cgcgatgcct
      841 gaatcgataa agatcggccc aattgaggct gttcgcaagt tcgtcgaggg gttcaacaac
      901 ggcgacgtcg agttagccca agcagcctgt gcacatcaga tctcgatcat cgacgacttt
      961 ccgccgtacg gttggcaggg aggcggagcg atgacggggt ggtttcgcga cttggcccga
     1021 atgagcactg agcacggaat gtcagatcct tgggtgactt tacgcgaacc actccaagtg
     1081 gaaatgaccg accgcaaggc gtacatggtg gttccgatcg acgtcggctg gcttcagaac
     1141 ggagcgcgcg cagagagaac gggcttcatg acgattgtgc tctgggagga ggccgagggt
     1201 tggcgcatct ctgcatgcgc ctggacctgg aactaggagc ctctatccag acccaatcca
     1261 ggcctgacta gccctcctca ccgacctccg ctcggacggt cctcccgctt cgcaggggga
     1321 caaacgtgct cgaggtcagg gaattggcac caggccgatg tcaccctcga ggacgaccgc
     1381 gacttcaccc ggctcgtcct ggatatccca ttgctcgaag aaggcactgg cgccttcccg
     1441 actctcccag ttggctaccg cgtagaacgc cgattcgtcg gaggcgtacc aggcccgatg
     1501 gaatcgacac ccatgctcga cggcgctggc ccgcatctcg tcagtgatcc tgtccacccg
     1561 cttctgatag agatccctcg ctgtctggtc gtcgggggtg ggacagggca cggagaacag
     1621 gatccacatg tcggcctcct ggtggttggt gggccgaggt tatcctcgcc gccctaggag
     1681 gaatcaaatg cgtattggca tcttgaccgg tgggggagac gtccccggcc tcaacgcctg
     1741 catcaaagcg gtggtgaatc gagtcgtgag tgaaggccac caggtagtcg gcgtccgacg
     1801 gggttggggc ggtctcgccg aaaccaatcc agacgacccg aagtcgggtg aggcgaatct
     1861 cgtcccactg gaccggatgg cggttcggac catcgaccgc agcgggggca ccttccttca
     1921 cagttcgcgg accaatccgg ccaaggtacg cactgccgag ctacctgact tcctggcggg
     1981 cgacttcagt ggcgacggcc ctcacgacat cacgccgcac gtgctgcgtg tcctcgagac
     2041 gcacaagatc gatgtcctga tcccgatcgg cggcgacgac accctctcct acgggctccg
     2101 tctccacgag gagggagtgc cggtgatcgc catcccgaaa acgatggaca acgacgtcca
     2161 cggcaccgat tactgcatcg gattctccac ctcggtgacc cggggcgttc agttcatcca
     2221 cagtcttcgc actgcggccg gctcccacga gcggatagcg gtggtggagc tgttcgggcg
     2281 ttacagcggg gagacgtcat tgatcaccgc gtacctgtcc ggtgtcgatc gggcgatcat
     2341 ctccgaggtc cccttcgaga tcgacaagct tgccgccttt ctccaagagg accaagcggc
     2401 caacccctcc aactacgcga tggtgacgat ttccgaaggg gcccgtttga tcgagggcga
     2461 catccatctg accggcgaag aggacgccta cggccatcgc aagctcggcg gaatcggcgc
     2521 cacaaccggc gagctcctca aggagcggac cggcgagggg atcctctatc agcagatcgg
     2581 ctacctgatg cgatcgggtg cgccggactc cctcgacctg atggtcgcca ccaactacgc
     2641 ggtaatggct gccgacctgg caatggaagg ggcccacggg cggatggtcg ccctgaggag
     2701 cggcacctac accgacgtgc cgatcggggt tacccgagaa ggcgtgaaac gggtcgacgt
     2761 ggacgagttg tacgacgtcg acgcctatca gcccaaggtt cgccacgtcc tgggcaagcc
     2821 gatgttcctt tactgagaga tcaggaggcc cgacgcaggg cctcttcgat gtcggtccga
     2881 atctcctgcg gatcggcgcc catatttgcg aagactctgc ccagggggtc tgctgcgggc
     2941 aaagagagca atgcgagcag gacatgttcg gtcccaatct gacgcccacc ctgcttgatc
     3001 gtctcccgca gagtgtcggt gagcaccgcc tttgcgccgt gggtgaaggg gagatgacgt
     3061 cgagcgtggc gcgggaccgc gccgtccaga gcccgcagtg ccggatcgaa aagctcgaga
     3121 tccacgccca ccgccgcaag ggactccgag tcaagtcgat ctagttgtcg tcgaatctct
     3181 tcgcttccca catccaaagg tccgagtagg gagcgctgga aagggaaact caccccggcc
     3241 actgcaatca ggaggtgctc cgttccaacc ctgctgtcat ctcgcgcctc ggcctcggcc
     3301 acggcggtgg tcacgatccc gcgagcctct tttccgaatc tctcgaacat ctctatctcc
     3361 ctgacttctt gtgaattgcc tgacgggaga cacccagggc gtctccgatc tgttgccatg
     3421 tccagccctg gcgccgggcg gaggcaacgt ggatctcttc gaggcggtct gcgagacgac
     3481 ggagggtgac caccgcccgc aaacccaagg cagggtcggc gccttcggca gctaacaagg
     3541 tcttgttgag atccacggct gtcagcttag gttgacagga tgaagtttgt caatctctgt
     3601 tgacaaagtt tctcccccca ggaggttcag cgacgttggg gggaggggac cgccgaaggc
     3661 ggtgaggggg gcctctcgcg cgcgatgtga gaggacttgt ctattagccg cgtctcgccg
     3721 cggccacccg aaacagggtg cgagcacctt ccccgagaac cgagaaccgt agatccttgg
     3781 tctggccacg cacccaacgg atgtagatct gctgcagaac gactcccaac ttccaggtgc
     3841 cgaagacgat gtaccagtcg atggagtcga gggcccggcc cgacctttcg ccgtagcggg
     3901 cgaccgctga tcccctactg atccaacccg gagcatcggt cggcatcggg ttgagagccg
     3961 accgctcctc accggggtcg taccaagatg ccatcagggt tccgagatca gcgagaggat
     4021 caccccgggt ggccatgtcc cagtcgtaga cggccacaca tttcccggga tcgtcggggg
     4081 caaccgcgag attgtcgagc cgccagtcgt tatggagcag ggtcacctcc gtggactcgg
     4141 ggagattggc caccagccac ccggaaactt cttcggccac cgggctgtcc tcgtctttgg
     4201 cctgattcca gcgttcggtc cagccatcca cctgccgcgc cagaaagtcc tcaggatgcc
     4261 cgaggtcgcc cagcccggct agcaccgggt caaccccgtg gaactcggca agggtgtcga
     4321 ccacgacctc ggacagtttg cgattggacg ctggatcgtg cccgcctccg aacaggggag
     4381 ggatggtgtc acgaaccacg actccatagc ggcggtccat catgaaaaag ggagagccga
     4441 ggacggaaac gtcatcgcaa taggcgaggg cccggggcgc cttggggaag acgctccaca
     4501 gactggagag aacccggtac tcacgggcca tatcgtgggc tccgggcgca accgggccca
     4561 gcggaggtct ccgcagcacc aactcggtcc cgtcgggaaa ggtcaggaga taggtgaggt
     4621 tggccttgcc accggcgaac tgcccgacga gaggagcgcc tcgagggaga tcgggaatgg
     4681 tctcgagcca gtcggcgagg cgatcgacgt cgaaaccctc gtcgtctcgg attgggatga
     4741 tgtcgcccat cgggtcgagg gtatccaaca ggtgggaacg gccgtctagc ctcggttgcc
     4801 ccggctgagg agaccatgac cacccccttc gagatttctg accgattcac agagcaatgg
     4861 tccgacctga caccgatcga cgccacgatc cagggcgtcg agggtcgtga tcacctgtgg
     4921 gacgacttct ccccagaagg cgaagcggcc cgggccgatc tcgcccggtc caccagggga
     4981 gagctggttg aacatctgaa ccatcccgat cccaagcagg cgtttgcggc gaaggtgctt
     5041 gctgcctgga tgatgaagaa ggtgacggtc ttcgagcggg gcaagtggaa gagcgacctc
     5101 aaccatatct acagcccatt ccagcgcgcc cgcgacacgt ttgatgtcat gcccaagcaa
     5161 ggcgaggagg cctggtcaaa cattgccacc cgtctcgaga ctttcggcca ggtcaccgag
     5221 ggctatcggg cggcgctcca ggtcgggctc gatgttggtg tgaccgccgc tcagcgtcag
     5281 gtgagaacgc tgatcgacca agcacgagct gtggcggctg aagactcgcg attcgcgggc
     5341 tatccgactg aggcatcgag cagcggcggg gatccgactc gggttgcgaa cgcgg
//
LOCUS       DBNHXU010000072         1755 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_617651, whole genome shotgun sequence.
ACCESSION   DBNHXU010000072 DBNHXU010000000
VERSION     DBNHXU010000072.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1755)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1755
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_617651"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="AC161X_01950"
     CDS             <1..516
                     /locus_tag="AC161X_01950"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR4988344.1"
                     /translation="ARGLESFVYLTVGTGIGGGAVSGGRLVSGAFHPEMGHVVVNARL
                     SDDFEGLCPYHRSCLEGMASGPAIQARYGIRAPDLGADMAEAVDLVGFYLAQGIRDIV
                     YILSPQRVVVGGGVSKLPGFIDSIESHLVEQLAHYPGHPEYGPGFVVLPALGDLSGLA
                     GAAVLAERALA"
     gene            518..1708
                     /locus_tag="AC161X_01955"
     CDS             518..1708
                     /locus_tag="AC161X_01955"
                     /inference="COORDINATES: protein motif:HMM:NF024622.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HIR4988345.1"
                     /translation="MGTPHLQIRPGNPDFLDLPWADPIEEWGSERLVEMPTGVHRHPV
                     VFVAYEEGVYAIKELPRRRAASEFSVLRSLEDRTGVAATPAGLVSRDWLDGGAEQGGA
                     VITRYVEHSFPYRHLVSGPGFGRRRGQLLDAIAGLLVELHLAGCFWGDCSLSNVLYRY
                     DAGAIEAIMIDAETSELHPALSDGQRAQDLEIMKENLAGEMADIAAEHELDIDEADLG
                     LGDGVEERYNALWFELDQDLVISSSERYLIRKRIARLNELGFSVDDVDLAPVGGDDRV
                     RMRVRVGGRTFHSQRLRELTGVEASENQSRHILSDLNYFIAKSNSKSATGKTVAVMSW
                     ITTCFEPLTARVAEVWQGDPIQGFCDLLQFRLSVATERGSDVPNDEAFRLWSDAGFPG
                     FPVR"
BASE COUNT          279 a          523 c          603 g          350 t
ORIGIN      
        1 gctcgaggtt tggagagttt cgtttatctc accgtcggga ccgggatcgg cgggggagcc
       61 gtttccggtg gtcgtctggt cagcggggcg ttccatccgg agatggggca cgtggttgtc
      121 aacgcccgcc tgagcgacga cttcgaaggg ctatgcccct atcaccgttc ctgtctcgag
      181 ggcatggcta gcgggccggc gatccaggcg aggtacggga tacgagcgcc ggacttggga
      241 gccgacatgg ctgaggcagt cgacctggtt gggttctatc tcgctcaggg gatccgtgac
      301 atcgtgtaca tcctcagccc gcaacgcgtg gtggtcggtg ggggagtctc caagttgcca
      361 ggcttcatcg actcgataga gagtcaccta gtcgagcagc tcgcccacta cccgggtcat
      421 ccggagtacg gaccgggttt cgtcgtgctt ccggcgttgg gggacctttc tggcctggcc
      481 ggggctgctg ttctcgccga gagggcgctg gcctgagatg ggaacccctc atctccaaat
      541 tcgcccgggc aacccggact ttttggacct cccctgggca gacccaatcg aggagtgggg
      601 ctccgaacgc ttggttgaga tgcctaccgg agtccatcgt cacccggtcg tgtttgtcgc
      661 gtatgaagag ggggtgtacg ccatcaagga gctgccccgt cggcgggcag cctcggagtt
      721 ctcggtgctc cgatcactcg aggatcggac cggcgtggca gccaccccgg cgggtttggt
      781 ctcaagggac tggctggacg gcggggcgga gcaaggcggg gcggtgatca cccgatatgt
      841 ggagcactcc ttcccttacc ggcacctggt gagcggcccg gggttcggtc gccggagggg
      901 ccagttgctc gatgcgattg ccggcctctt ggtagagctc catcttgccg gctgcttttg
      961 gggcgattgt tcgctctcca acgtcctcta tcgctacgac gccggcgcga tcgaggcgat
     1021 catgatcgac gccgagacct ccgagctgca tcccgcgctc tccgacggac agcgtgccca
     1081 ggatctggaa atcatgaagg agaacctggc cggtgagatg gcggacatcg ccgcagagca
     1141 cgagcttgac atcgacgaag cagatctcgg gctaggcgat ggggtcgagg agcggtacaa
     1201 cgcgctctgg ttcgagttgg accaggacct ggtgatctct tccagcgagc ggtacctgat
     1261 tcgcaagcgg atagcccgcc tcaatgagct ggggttctct gtcgacgacg tcgacttggc
     1321 cccggtaggg ggtgatgacc gggtccgcat gagggtccgg gtggggggtc gcacctttca
     1381 cagccagcgc ctgcgtgagc tgacgggggt cgaagcctcc gagaatcagt ccaggcacat
     1441 tctcagcgat ctgaactact tcatcgccaa gtcgaacagc aagtcggcca ccggcaagac
     1501 ggtcgccgtc atgtcgtgga tcacgacctg cttcgagcca ctgacggcac gagtcgccga
     1561 agtgtggcag ggcgatccga tccagggttt ctgtgacctg ctccagttcc gtctctcggt
     1621 ggctaccgag cgcgggtcgg acgttcccaa tgacgaggcg ttccggctgt ggtccgatgc
     1681 cgggttcccc gggttcccgg tgcgctgatc ggctagaccg tcctggtcac cttgttcagc
     1741 ccgcgcggct gctca
//
LOCUS       DBNHXU010000073         1526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_22816, whole genome shotgun sequence.
ACCESSION   DBNHXU010000073 DBNHXU010000000
VERSION     DBNHXU010000073.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1526
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_22816"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1507)
                     /gene="pdhA"
                     /locus_tag="AC161X_01960"
     CDS             complement(<1..1507)
                     /gene="pdhA"
                     /locus_tag="AC161X_01960"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR4988346.1"
                     /translation="MARRGGLGRGLEALIPTETAEQSRKRLLRIIDANGELVGDDPGL
                     DPELYQQMYRRMVLARTLDRKMLALQRQGRLGTYAMLEGQEAVQIGSALALKPDDFVF
                     PSYREHAVQVTRGLPIEVLLAYWKGLPNTGWDLNKFRTGIVTVPIATQLPHAVGYSYV
                     TKLRGEDTVTAVYFGDGATSEADFHAAMNFAGVWKTPTVFICANNLYAISVPYEKQTA
                     SETIAQKAAAYGFDGFRVDGMDPIAMYLATRIAVRRARKGQGPTLIEAMTYRYGPHAT
                     ADDPTLYRSREEEEKWRQRDPIERLRLFLEGRGEWDERVGEKVAQETADQVEAAITAI
                     ESEPLPGRDDAIRHGFQRIPAHVIDQLHGMQEAHGEEPTAFGEEDVWTIGHDQPVSGP
                     TESWTMADAINAALGEAMERNPEVIVLGEDVGLSGGVFRITEGLQEKYGEGRVIDTPL
                     NESGIVGSAVGMALAGARPVAEIQFEGFVYPAFDQIVSHLGRFRYRTRGNSS"
BASE COUNT          254 a          497 c          471 g          304 t
ORIGIN      
        1 cgctggaatt gccccgggtc cggtaacgga accttcccaa gtggctgacg atctgatcaa
       61 aggccggata gacgaagcct tcgaactgga tctcggccac cggtcgagcg ccggccaggg
      121 ccatccccac cgcgctgcca actatcccgg actcgttgag tggggtgtcg atgacacggc
      181 cttcgccgta tttctcttga agtccttcgg tgatccgaaa cacgcccccg ctgagaccaa
      241 cgtcctcgcc gagaacgatc acctccgggt tgcgctccat cgcctcgcca agggcggcat
      301 tgatggcgtc ggccatagtc cacgactcgg tggggccgga cactggctga tcgtggccaa
      361 tggtccagac atcctcctcg ccaaaggcgg tcggctcctc gccgtgtgcc tcctgcattc
      421 cgtgcaactg gtcgatcaca tgcgcgggga tgcgctgaaa cccgtggcgg atagcgtcgt
      481 cgcggccggg gagcggctcg gattcgatcg ccgtgatcgc cgcctcgacc tgatctgctg
      541 tctcctgggc gaccttctcg cctactcgct cgtcccattc gccgcgaccc tcgaggaaga
      601 gccggagtcg ctcgattgga tccctttggc gccacttctc ctcttcctcc cgtgaccgat
      661 agagggtggg gtcgtcggcc gtggcgtgtg ggccatacct gtaggtcatc gcctcgatca
      721 gggtcgggcc ctggcccttg cgcgctcgcc gtaccgcgat cctggtcgcc aggtacatgg
      781 cgatcggatc catcccgtcg acccgaaaac cgtcgaatcc ataggccgca gccttctggg
      841 cgatggtctc cgatgccgtc tgcttctcgt acggcaccga gatcgcatag aggttgttgg
      901 cgcagatgaa cacggtcggg gtcttccaga ccccggcgaa gttcatggcg gcatggaagt
      961 cggcttccga agttgctccg tcaccgaagt aaacggcggt gactgtgtcc tcacctcgga
     1021 gttttgtgac gtaggagtag ccaacggcgt gggggagctg ggtcgcgatc ggcaccgtca
     1081 cgatcccggt tcgaaacttg ttcaggtccc aaccggtgtt ggggaggcct ttccaatagg
     1141 cgagcaagac ctcgatgggg aggccacggg tcacctggac cgcatgctcc cgataggagg
     1201 gaaaaacgaa gtcatccggt ttgagggcga gcgcagagcc gatctgcacg gcctcctggc
     1261 cctcgagcat ggcgtaggtc ccgagacggc cctgacgctg caatgcgagc atcttccggt
     1321 cgagggttct agccaggacc attcgccggt acatctgctg atagagctca gggtcgagcc
     1381 ctgggtcgtc tccgaccaac tcgccgttgg catcgatgat tcggagcaac cgcttgcgcg
     1441 actgctccgc agtctcggta ggtatcaggg cctccagacc ccgacccaat ccgcctctcc
     1501 gtgccaacgc ctgttctctc ctttcg
//
LOCUS       DBNHXU010000074         3708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2206723, whole genome shotgun sequence.
ACCESSION   DBNHXU010000074 DBNHXU010000000
VERSION     DBNHXU010000074.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3708
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2206723"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="AC161X_01965"
     CDS             complement(<1..776)
                     /locus_tag="AC161X_01965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988347.1"
                     /translation="MAEIPESLLRRSAEAKAKALGLPVEQVLAEMKGEAPPPQPPAPE
                     PAPEPALVEEAVEEVPPASAAPVEEPVVEVASDPVVTPEAPHEAPADVAAPAEPEPDL
                     APVEAAEEAGSSEPVAPAVEETAAASAETELAPAGVSTLVEDQPTPAEGNGAGNGKVA
                     STVSPRPVLPPAGTPDGVRTQRLLTVVKARAIQTVKAEPTDKVNTWPHLMLMEFGALL
                     AITGFLVILAVIIHSPLLAPANFNATPNPSKAPWYFLGLQ"
     gene            complement(787..1533)
                     /gene="extP"
                     /locus_tag="AC161X_01970"
     CDS             complement(787..1533)
                     /gene="extP"
                     /locus_tag="AC161X_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008479046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenite/tellurite reduction operon b-type
                     cytochrome ExtP"
                     /protein_id="HIR4988348.1"
                     /translation="MDRVKEANEKLWGSQLAESILRPGSPFKKGYSDSPRNRSYVVMN
                     NVLYHLHPVKVKRHGVRLSYTLCLGGLSFFLFILLTITGIFLMFYYTPAAQTAYPDIE
                     ALSTNVAFGSLVRNMHRWGAHLMVLSVFLHMSRVFYHGAYKPPREFNWVVGVVLLFLT
                     LLLSFSGYLLPWDQLALWAVTVGTNMAGFVPVVGAQVKFALLGGAEVSAATLLRFYVL
                     HVLALPFILVIFLAVHFWRVRKDGGISGPL"
     gene            complement(1572..2021)
                     /locus_tag="AC161X_01975"
     CDS             complement(1572..2021)
                     /locus_tag="AC161X_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HIR4988349.1"
                     /translation="MVRGVVDETPPIRDDYQLAVVEGDYFTDKLRPKQFLHIDQAECI
                     ICEGCVDICPWKCIHYLSLDAITEGVNVDHPADDPQNLGFFIVDEDECTRCALCVDRC
                     PTNVISLGKFEGSLADQAVELGLDRRPWDFDTGQRDFKNGYAYGMRW"
     gene            complement(2021..2653)
                     /locus_tag="AC161X_01980"
     CDS             complement(2021..2653)
                     /locus_tag="AC161X_01980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988350.1"
                     /translation="MYRLAELSVSTRRKAGWGLLLLGFAALVLGVIWIHFSSLSETVV
                     VDGVEVPLVVDYFNWWPRGTLWQGVGYLVVFAASQMMILGAVFLWVLNQRMTWSLAIF
                     LAVLSWLELVIIFGMVPSEWLSYAQTELDWSPQKIFLVVPPVLVLGNTVEISYAVVKD
                     SISMGYHLVMLAVAAIFTLQVQKIYKGRPESAQKPDKKSPYGRPLVKGGT"
     gene            2816..>3708
                     /locus_tag="AC161X_01985"
     CDS             2816..>3708
                     /locus_tag="AC161X_01985"
                     /inference="COORDINATES: protein motif:HMM:NF012343.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HIR4988351.1"
                     /translation="MATSQSADIRAFLGGGADEDRVAAAHFIVGAVFLVLGGLLELLA
                     LFALRFGGLSPISLGRVEPMANLALMIGFLVISLVGGVYYVLPRLTGAPLRSPAMARG
                     GLALLVVMVLAGLGAIAFGFGDGSEPFGLPWWLDVPLLAALSLPALNTLATIARRQET
                     RTYVTLWFVIGGVVWLPLLFAAHVVWTLPFADSLAQTYSGLFFSAGFVTMWVFTVGTG
                     LFYYTLVKEVDVPLASRQLAVAGFWSLGFAAVWWGMAQLIFGPGPGWADAVAAALGLA
                     FPIGALANASNVSLTLQGHWDR"
BASE COUNT          686 a         1149 c         1171 g          702 t
ORIGIN      
        1 tcctgcaggc caaggaagta ccagggtgcc ttcgacgggt tcggagtggc gttgaagttg
       61 gccggggcca gcagaggcga gtggatgatg accgccagga tcaccaggaa cccggtgatg
      121 gcgagcagtg ccccgaattc catgagcatc aggtggggcc aggtgttcac cttgtcggtg
      181 ggctccgcct tgaccgtctg gatggcacgg gccttgacca ctgtgagaag gcgctgggtt
      241 cggacgccat ctggagtccc ggccggggga aggaccgggc gagggctgac ggtggacgca
      301 accttcccat tgccggcgcc gttcccttcg gcgggcgtcg gttgatcttc gaccagggtc
      361 gacactcctg ccggcgcaag ttcggtctcg gctgaggccg cggcagtctc ttcgaccgca
      421 ggtgcaaccg gctccgagct cccggcttcc tcggcagctt cgaccggcgc caggtccggc
      481 tcaggctcgg cgggggcggc gacgtcggcc ggggcctcgt gcggcgcttc gggggttaca
      541 accggatcgg aagcgacttc gacgactggc tcctccactg gagcagccga tgcaggggga
      601 acctcttcca cggcttcctc tacaagagca ggctcgggag ccggctccgg agcgggaggc
      661 tggggcggag gggcttcacc cttcatctcg gccagcacct gctcgaccgg aagacccaaa
      721 gccttggcct tggcctctgc cgatctcctg aggagactct ctggtatctc tgccattcaa
      781 tgctccttag agagggccgg aaatcccgcc gtccttgcgg acccgccaga aatggaccgc
      841 gaggaaaatc acgagaatga acggcaacgc gaggacatgg agcacataga aacggagcag
      901 ggttgctgcc gacacctccg ccccgcccaa aagggcgaac ttgacctggg ctcccaccac
      961 cggcacgaat cccgccatgt tggtcccgac cgtgactgcc cacagcgcca gctgatccca
     1021 gggcaggaga tagccggaga aggaaagcaa cagagtcagg aacaacagga cgacaccgac
     1081 cacccagttg aactcgcgcg gcggcttgta agcgccgtga taaaagaccc ggctcatgtg
     1141 gagaaacacc gaaagcacca tcaggtgtgc cccccatcgg tgcatgttgc gcaccaatga
     1201 gccaaaggcc acatttgtgg aaagtgcctc gatgtcggga taggcggtct gcgccgcagg
     1261 cgtgtaatag aacatcagga agatgccagt gatggtgagc aggatgaaga ggaagaagct
     1321 gaggccgccc aagcagaggg tgtaggacag ccgaactccg tgtcgcttca ccttcaccgg
     1381 atggaggtga tagaggacgt tgttcatcac cacataggac cggttcctcg ggctgtccga
     1441 gtagcccttc ttgaaaggtg agccgggacg aaggatcgat tcggcgagtt gggagcccca
     1501 cagcttctcg ttcgcttcct tcacccggtc catgagaccg acttttccgt tgccgttagc
     1561 caagagctct cctaccagcg catcccatag gcgtacccat tcttgaagtc acgttgcccg
     1621 gtgtcgaaat cccagggccg acgatcgagg ccaagctcga ctgcctggtc ggcgagggat
     1681 ccttcgaact ttccaaggga gatgacattg gtcggacagc gatcgacaca caaagcgcaa
     1741 cgcgtgcact cgtcctcgtc gacgatgaag aagccgaggt tctgcggatc gtcggccggg
     1801 tgatcgacat tgactccttc ggtgatcgca tccagggaga ggtagtggat gcacttccag
     1861 gggcagatgt cgacgcatcc ttcgcaaatg atgcactccg cctggtcgat gtggaggaac
     1921 tgcttggggc gcagcttgtc cgtgaagtag tcaccctcca ccacggcaag ttggtagtca
     1981 tcccgaatcg gcggtgtctc gtctacgact ccgcgaacca tcaggtccct cccttgacca
     2041 ggggacgacc gtaaggcgac ttcttgtcgg gcttctgtgc cgactccggc cggcccttgt
     2101 agatcttctg gacttgcagc gtgaagatcg ccgccaccgc aagcatcaca aggtggtagc
     2161 ccatcgagat cgagtctttg accaccgcgt agctgatctc aacggtgttg cccaacacca
     2221 gcaccggagg caccacaagg aagatcttct gcggtgacca atccaattcg gtctgcgcgt
     2281 aggagagcca ctcggagggg accatgccga agatgatgac cagttccagc caggaaagga
     2341 ccgcgaggaa gatcgccagc gaccaggtca tcctttgatt gagcacccag aggaaaaccg
     2401 cgccgagaat catcatctgg gatgcggcaa agacgaccag gtagccgaca ccctgccaca
     2461 aggtgccgcg gggccaccag ttgaaatagt cgacgaccag cggcacttcg acaccgtcga
     2521 cgacgaccgt ttccgacaag ctcgagaagt ggatccagat caccccgagg acgagggccg
     2581 caaacccaag cagaagtagg ccccagccgg cctttctgcg ggtgctgaca ctcagttctg
     2641 cgagtcggta catctctgtt gtttcgggcg cggatctagt ggctgtcggg cggtttctac
     2701 cataaccggg acccgcctac agaacccgaa tccgcaaggc ccccaaatgg tcgaggacgg
     2761 aacgaatcag cgaagtcgtt atgctcccgc cgtcctgtcc tgaacgagga atccggtggc
     2821 gactagccaa tcagcagaca ttcgagcctt cctcggaggc ggggccgacg aggaccgcgt
     2881 agcagcggca cacttcatcg ttggagcggt cttccttgtc ctcggcggtc tgctcgagct
     2941 cctggccctg ttcgcgcttc ggttcggggg gctgtctccc atcagcctcg gccgggtgga
     3001 accgatggcc aacctcgccc tgatgatcgg cttcctcgtg atatccctgg tcggcggtgt
     3061 ctactacgtg ttgccacggc tcaccggcgc accattgcgg agcccggcaa tggcccgcgg
     3121 cggtctcgcc ttgctggttg tcatggtgct ggccgggctg ggggcgatcg cattcgggtt
     3181 cggcgacggg tccgagccat tcgggcttcc ctggtggctc gatgtgcctt tgttggcagc
     3241 cctctctctt cccgcgctca acaccctggc gaccattgcc cgccgtcagg agacgcgcac
     3301 ctacgtgacg ctttggtttg tgatcggcgg tgtcgtctgg ttgccgctcc tcttcgctgc
     3361 ccacgttgtg tggacccttc ccttcgccga ctccctggcg cagacctact cagggctgtt
     3421 cttctcggcg ggtttcgtga cgatgtgggt cttcacggtc gggaccggcc tcttctatta
     3481 cacgctggtc aaggaagtcg acgtgcccct ggcttcgagg cagttggccg tggccggttt
     3541 ctggtcgttg ggctttgccg ccgtctggtg gggtatggcc cagctgatct ttggcccggg
     3601 gccgggctgg gccgatgccg tcgccgctgc cctcggcctt gccttcccta tcggggccct
     3661 ggccaacgcg tcgaatgtct ccctcaccct gcaaggtcac tgggaccg
//
LOCUS       DBNHXU010000075         9476 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3198029, whole genome shotgun sequence.
ACCESSION   DBNHXU010000075 DBNHXU010000000
VERSION     DBNHXU010000075.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9476)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9476)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9476
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3198029"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1074
                     /gene="pheS"
                     /locus_tag="AC161X_01990"
     CDS             25..1074
                     /gene="pheS"
                     /locus_tag="AC161X_01990"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HIR4988352.1"
                     /translation="MDVIAELEDALSGAAARVDGAGSVEELETVESSLLGKNSVVGSV
                     RRAMGGLEPSQRPVVGARLNEVTTEIDRLIAQRRVALSILDEQHRLTGDRIDVTLESV
                     NFPRGSLHLIQQTIDEVVDIFVGLGYLTTSGPEAELGWYNFDALNTPPTHPSRLESDT
                     MYLDYGDPADELLLRTQTSPSQVRWMQQNPPPVYVVVPGKVYRTDTIDASHLPVFHQI
                     EGLAVDDSITFSDLKGTLTHFAKEFFGSRTTVRLRPHFFPFTEPSAELDVSCFACDGG
                     EAGCRVCGGAGYLEMLGCGMVDPNVFEAVGYDPSSLSGFAFGMGVERLAMVRHGINNI
                     RHFIDNDLRFLRQFS"
     gene            1071..3455
                     /gene="pheT"
                     /locus_tag="AC161X_01995"
     CDS             1071..3455
                     /gene="pheT"
                     /locus_tag="AC161X_01995"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HIR4988353.1"
                     /translation="MKVPLRWLSEFITLPELSPSELSDIIAMIGHEVEGFEVLEADWS
                     DVVVGQVVDIKAHPDADKIRVCQVDVGAGPEQIICGAWNFENGAYVAVARPGAVLPGG
                     FEIGQRTIRGVDSNGMICSDRELGLGDDHSGILVLDGEPSLGIDFSELVELPDVVFDI
                     TVTPNRPDAMSLRGIARDLGAYLDLPYRTPERELNTVPGATGIEVELGDPVGCRRFTA
                     REIRGVSVGKSPLWVRHRMAKAGMRAISNVVDVTNYVMLELGHPLHAFDADSIVGRHL
                     VVKRAAAGEQLVTLDDETRTLSHEDLIIYDEEGPTSMSGTMGGARSEVSDGTVNVLME
                     AASWDPPTIMYMSRRHNLRSEASTRFERGVDPNLADEANRRATAMVAELAGGEVPEGA
                     IDVVGTVVDPATIRFELAEVTRLLGPGFTHEEVSAILGRLGMSVEGEDPMTVVVPTFR
                     PDVTRPADLVEEVARIHGFDKFDATVPTGPAGGLTPEQKRLRVLHRSLVGIGISQAVT
                     LPFVGEEDVLAFGVSLGDVLRVKNPLRDEEGRLRPTMLPSLLNAVRFNLSHGASSVAL
                     FETGRVFAARPGVDDSRLPEQVERLAWVAVGPVGVSTLDEPGLEADGRISLGILRHVA
                     GALGLEGIGLTAATPPGYHPGRTAAVTLNGSAIGYVGELSPTVASGYEILGRVAVAEL
                     DLDPLLASPGLVQSESPSVYPPVDFDLSFLVADSIPVSSVLGATVDAGGAMVESAHVF
                     DEFKGAGIPDGSRALAIRYRLRAPDRTLTNEEVSPVRGAMIEAATAVGAQLRGA"
     gene            3467..4024
                     /locus_tag="AC161X_02000"
     CDS             3467..4024
                     /locus_tag="AC161X_02000"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015804152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HIR4988354.1"
                     /translation="MELAAQLAGSPEVAAPLLLGAVLVSTIGSSSVAVRLNEVEAYRG
                     EDDPASHAFKGRTGRNGSMFERPGTLYVYRSYGMHWCANVSAGPEGTGWAILFRGGEV
                     VDGVGAARARRGRDTELANGPGKLSQALGIDGSHDGTNLFDDDSPLRLEPGSPPQLVM
                     ATPRIGISKARDLPWRFIVATSTAN"
     gene            4067..5257
                     /gene="tyrS"
                     /locus_tag="AC161X_02005"
     CDS             4067..5257
                     /gene="tyrS"
                     /locus_tag="AC161X_02005"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013495261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HIR4988355.1"
                     /translation="MTPADQLPYLLRHTDKVVPEDELLEKLKSGRQLRVKLGLDPTAP
                     SVTLGWAVVLRKLRHFQEMGHTAVLIVGDFTARVGDPSLRSDTRKRLTAEEVNGYAEN
                     VLGQFRKVLLDERLEIRRNSEWLGALDMGGLLELTSAATVAQMLERSDFANRYQEGSP
                     ISVMEFLYPLLQGYDSVAVEADVELGGSDQLWNLMMGRVVQERYGQEPQVALTMPLLV
                     GTDGSKKMSQSIGNYIGVEDPPSDMFGKTMSIPDQVMPEWFRLAADFDPAEVEGIESG
                     LASGELHPGEVKRQLARAVTALYWGEEAAAEAEAGFDQVFRDRGIPDDIEDFSLPAGD
                     TVQMSALVREVSGESGSEARRLLAQGAVRLDGEAVADGEMAREALVGRVLKVGKRRFV
                     RLAE"
     gene            5613..7141
                     /locus_tag="AC161X_02010"
     rRNA            5613..7141
                     /locus_tag="AC161X_02010"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
     gene            7417..>9476
                     /locus_tag="AC161X_02015"
     rRNA            7417..>9476
                     /locus_tag="AC161X_02015"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT         1933 a         2651 c         3064 g         1828 t
ORIGIN      
        1 cgaccgctct aacctgccgc ccacatggat gtgatcgcgg agctggaaga tgctctttcc
       61 ggagccgccg ccagagtcga cggcgcaggg tccgtcgagg agctggaaac ggtcgagagc
      121 tcccttctcg gcaagaactc tgttgtcggc tcggtccgac gcgccatggg cggcctggag
      181 ccatcgcaac gacctgttgt gggtgcccgg ctcaacgaag tcacaaccga aatcgatcgc
      241 ctgattgccc aacgccgtgt cgccctctcg atcctcgacg agcagcatcg actgaccggt
      301 gaccgcatcg acgtgactct ggagtcggtc aacttccccc gcggatctct gcacctgatc
      361 caacaaacca tcgacgaggt cgtcgacatc ttcgttggcc tcgggtacct gaccacctct
      421 ggccctgaag ccgagctcgg ttggtacaac ttcgatgccc tcaacacacc gcccactcac
      481 cccagccggc tggagtcgga caccatgtac ctggattatg gggatccggc ggacgagctg
      541 ttgctccgca cccagacgtc accgagccag gtccgatgga tgcagcagaa cccccctccc
      601 gtgtatgtgg tggtgccggg aaaggtctat cgcaccgaca ccatcgacgc ttcgcatctc
      661 ccggtattcc atcagatcga agggctggcg gttgacgact cgatcacctt ctccgacctc
      721 aagggaacac tgactcactt cgccaaggag ttcttcggct ctcggaccac ggttcgcctc
      781 cgcccccact tcttcccctt cactgagccc agtgccgagc tcgacgtctc ctgtttcgcc
      841 tgcgacggcg gtgaggcagg gtgccgggta tgtgggggag cggggtactt ggagatgctc
      901 ggttgcggga tggtcgatcc gaatgtgttc gaggcggtcg gatatgaccc gtcgagtttg
      961 tctggattcg ccttcggaat gggtgtcgag cgtctcgcca tggtccgtca cgggatcaac
     1021 aacatccggc acttcatcga caacgacctg cgctttctga ggcagttctc gtgaaagtcc
     1081 cgctccgatg gctctccgag ttcatcacgc ttcccgagct ctcgccctcc gagctgtccg
     1141 acatcattgc catgattggg cacgaggtgg agggcttcga ggtgctcgaa gccgattggt
     1201 ctgacgtcgt tgtgggtcag gttgtcgata tcaaggccca tccggacgcg gacaagatca
     1261 gggtgtgcca ggtcgacgtc ggtgccggcc cggagcagat catctgtggc gcctggaact
     1321 tcgaaaacgg ggcttatgtc gccgtggccc ggcccggcgc ggtgcttccc ggtgggttcg
     1381 agatcgggca gaggacgatc cgtggcgtcg attccaacgg gatgatttgc tcggatcgcg
     1441 agctcggtct cggcgacgac cactcgggga tcctggtcct cgatggtgag ccgagtctcg
     1501 ggatcgactt ctccgagttg gtcgagcttc ccgatgtcgt tttcgacatc actgtcactc
     1561 cgaaccgacc cgacgcgatg tcactgcggg gaatagcccg cgaccttggc gcctacctcg
     1621 accttccata tcgcacaccg gagcgtgagt tgaacactgt ccccggagcc actggcatcg
     1681 aggtcgagct tggcgacccg gtcggttgtc ggcggttcac agcacgtgag atccgtggag
     1741 tcagcgtcgg gaagtcgcct ctgtgggtga gacaccggat ggcgaaggcg gggatgaggg
     1801 cgatctccaa tgtcgtcgac gtgaccaact acgtgatgct tgagctcggc catcccctcc
     1861 acgccttcga cgccgattcg atcgtcggga ggcaccttgt cgtcaagaga gcagcggctg
     1921 gtgagcaatt ggtcaccctc gacgatgaga cgaggacgct gagccatgaa gacctgatca
     1981 tctatgacga agaggggccg acctcgatgt cagggaccat gggaggggct cgctccgagg
     2041 tctccgacgg aacggtcaac gtgctgatgg aggctgcctc gtgggacccg ccaacgatca
     2101 tgtacatgtc acgccggcac aacctccgct cggaggcatc gacccggttc gaaaggggcg
     2161 ttgaccccaa tctcgccgac gaggcgaatc gccgggccac tgccatggtc gcggagctgg
     2221 ccgggggaga ggtgcccgag ggcgcaatcg atgtcgttgg caccgtcgtc gacccggcaa
     2281 ccattcgatt cgaactggcc gaggtgaccc gcctgctcgg gcccggattc actcacgagg
     2341 aggtgagcgc gatcctgggc cggctcggga tgagcgtcga gggcgaagac ccgatgacgg
     2401 tcgtggtgcc aactttccga cccgacgtca ccaggcctgc agatctcgtc gaagaggtcg
     2461 cccgaatcca cggattcgac aagttcgacg ccacggtgcc taccgggccg gccgggggac
     2521 tgacgcccga acagaaacgg ctgagggtgc tccaccggtc gctggtaggg atcggcatca
     2581 gccaggcggt gaccctcccg tttgtcggcg aggaggatgt cctggccttc ggggtgtcgc
     2641 tcggcgacgt gctccgggtc aagaacccgc ttcgggacga agaggggcgg ctcaggccaa
     2701 ccatgctgcc aagccttctc aacgcggtcc gattcaacct ctctcacggg gcgtcgtcgg
     2761 tggccctgtt cgagaccggt cgcgtttttg cggcccgacc cggcgtcgat gactcgaggc
     2821 ttccggagca agttgaacgc ctggcgtggg tggcggtcgg gcccgtaggt gtgtcgacgc
     2881 tcgatgagcc tggcctggag gctgacgggc gcatttcttt gggaatcctt cgtcatgttg
     2941 ctggggccct tggcctcgaa gggatcggcc tgactgccgc cactccaccc ggataccacc
     3001 cggggcgaac cgcggccgtc acgctgaatg ggtccgcgat cgggtacgtc ggtgaattga
     3061 gcccgacggt ggcctcggga tacgagatct tgggccgggt cgcagtggcg gagctggatc
     3121 tggatccgct gttggcttcg ccagggctgg tgcagtcgga atcgccatcg gtgtatccgc
     3181 cggtcgactt cgacctctcg ttcctggttg ctgactcgat cccggtctcc tccgttctgg
     3241 gagcgacagt ggacgccggc ggggccatgg tggaatcggc gcacgtgttc gacgagttca
     3301 agggtgcagg gattcccgac gggtcgcggg cgttggccat tcgctaccgg cttcgagcac
     3361 cagaccgcac tctcaccaac gaggaagtgt ctccagtgcg tggggcgatg atcgaggcag
     3421 ccaccgccgt cggcgcgcaa ctccgcgggg cctgagcccg gtcctcgtgg aattagccgc
     3481 gcaacttgcc gggtcccctg aggttgcagc tccgctgttg ctcggggccg tcctggtttc
     3541 caccatcgga tcttcttctg tcgcggtgag gctcaacgag gtcgaggcct accggggtga
     3601 ggatgacccg gcatctcatg ccttcaaggg tcgcacaggg cgcaacgggt cgatgttcga
     3661 gcgcccgggc acgctctacg tctatcggtc gtacgggatg cactggtgtg ccaatgtctc
     3721 ggccgggccg gagggcaccg ggtgggcgat cctgttccgg ggaggcgagg tggtcgacgg
     3781 tgtcggggcc gcacgtgcga ggcgcgggcg cgataccgag ttggccaacg ggcccggcaa
     3841 gctgtcccag gccctgggta tcgacggttc ccacgatggc accaacctgt ttgacgacga
     3901 ctccccgctc cggctcgagc cgggctctcc accgcaactc gtcatggcca cacctcggat
     3961 cggcatctcc aaggctcgtg atcttccctg gcgcttcatc gtcgccacct caactgccaa
     4021 ctgaggctga ggcattgacc cggggtggtt taccctcgcg ccgaccatga cgcccgctga
     4081 ccagcttcct tatctccttc ggcataccga caaagtggtc cctgaggacg agctgctcga
     4141 gaagctgaag agcggacgcc agcttcgggt caaactcggg ctcgacccga ccgccccttc
     4201 ggtcacgctg gggtgggctg tggtgctgcg caagcttcgc cattttcagg agatgggtca
     4261 caccgcggta ttgatcgttg gcgacttcac ggcccgggta ggtgacccgt cactgaggag
     4321 cgacacccgc aagcgcctca ccgccgagga ggtcaatggc tacgcggaga acgttcttgg
     4381 tcagtttcgc aaggtcctcc tcgacgagcg actcgagatc cgacgcaact ccgagtggct
     4441 cggtgcgctc gacatgggtg ggctgttgga gctcacctcg gccgcaactg tcgcccagat
     4501 gctggagcgc tccgatttcg ccaaccgcta tcaggagggg agcccgatct cggtgatgga
     4561 gtttctctat ccgcttctcc agggatatga ctcggtggcg gttgaggccg acgtcgagtt
     4621 gggtgggagc gaccaactat ggaacctgat gatgggaagg gtggttcagg agcgttacgg
     4681 ccaggagcca caggtagcgc tgaccatgcc cctcctggtg ggaaccgacg gctcgaagaa
     4741 gatgtcccag tcgatcggca actacatcgg cgtcgaggac ccaccatccg acatgttcgg
     4801 caagacgatg agcatccccg atcaggtgat gccggagtgg ttccggcttg ccgccgactt
     4861 cgatcccgct gaggtcgagg gcatcgaatc gggtctggcc tccggcgagc tgcatccagg
     4921 tgaggtgaag cgccaactgg ctcgcgcggt caccgccctc tattggggag aggaggcggc
     4981 cgccgaggcg gaggcgggct tcgatcaagt cttccgcgat cgcgggatcc ctgacgacat
     5041 cgaggatttc tctctgcctg cgggggatac tgtgcagatg tccgccctgg tgcgagaggt
     5101 ctcgggggag tccggctcgg aggcgcgtcg gcttctggcg cagggtgcgg tccgactcga
     5161 cggagaggcc gtcgccgatg gcgagatggc cagagaggcc cttgtggggc gggttctcaa
     5221 ggtgggcaag cgccgatttg tccgcctcgc tgaatgaact tgactagatt tctgaggtcg
     5281 gtactattcg acgttcggcc cgggaatcac ttcccgggtc ccccaccacc cggtgaggga
     5341 attgtctcgc gttcaacgcg attgacgtat gtatcggggc agcgatctga tcgccggcct
     5401 gcctgacacc tggtgtggga gctttgggct ccttgacaac tgaacaatgt gccaaaagcc
     5461 agcaagcgct tgccatcagg gtcgattcgt cggctcggtg gtaggcattt tgcagcaatc
     5521 gaagctctcg gatctctcac agatccgaga cccaattgca aactcataac tgacaggatt
     5581 gggcatcgtc ttcggacgga gcccctgaat gtacgttgga gagtttgatc ctggctcagg
     5641 acgaacgctg gcggcgcgcc taatgcatgc aagtcgagcg acgcatattg gaggggtaac
     5701 ccaccgatat gacggagcgg cgaacgggtg agtaacacgt gagcaacctg ccccacagac
     5761 cgggatagcc cagggaaact tggattaata ccggataccc tcatggaacc acatggctct
     5821 ttgaggaaat gccttaacgc tgtgggatgg gctcgcggtt tatcagctag ttggtggggt
     5881 aacggcctac caaggcatcg acgaatagct ggtctgagag gacgatcagc cacactggga
     5941 ctgagacacg gcccagactc ctacgggagg cagcagcagg gaatcttgcg caatgggcga
     6001 aagcctgacg cagcgacgcc gcgtggggga tgaaggcctt cgggttgtaa acccctttca
     6061 ggagggacga aattgacggt acctccaaaa gaagccccgg ccaactacgt gccagcagcc
     6121 gcggtaatac gtagggggcg agcgttgtcc ggaatcattg ggcgtaaaga gctcgtaggc
     6181 ggttcagcaa gtcgtttgtg aaagtccgag gctcaacctc ggaattgcag tcgaaactgc
     6241 tgtgactcga gtccggtaga ggagaatgga attcccggtg tagcggtgaa atgcgcagat
     6301 atcgggagga acaccagtag cgaaggcggt tctctgggcc ggtactgacg ctgaggagcg
     6361 aaagcgtggg gagcaaacag gattagatac cctggtagtc cacgctgtaa acgttggaca
     6421 ctaggtgtgg cggtctgatc aacgactgcc gtgccgaagc taacgcatta agtgtcccgc
     6481 ctggggagta cggccgcaag gctaaaactc aaaggaattg acgggtcccc gcacaagcgg
     6541 cggagcatgc ggcttaattc gatgcaaccc gaagaacctt acctgggttt gacatcatgg
     6601 gaaaagccgt ggaaacacgg tgtccgaaag ggcccatgac aggtggtgca tggctgtcgt
     6661 cagctcgtgt cgtgagatgt tgggttaagt cccgcaacga gcgcaaccct tgtcctatgt
     6721 tgccagcggt tcggccgggg actcatagga gactgccggg gataactcgg aggaaggtga
     6781 ggacgacgtc aagtcatcat gccccttata tccagggctg cacgcatgct acaatggtcg
     6841 gtacaacggg cagcgatcct gcgaaggtga gcgaatccca caaagccggc ctcagttcgg
     6901 attggagtct gcaactcgac tccatgaagt cggagtcgct agtaatcgtg gatcagcaaa
     6961 gccgcggtga atgcgttctc ggggattgta cacaccgccc gtcaagtcac gaaagtcggc
     7021 aacacccgaa gtcagtggcc taaccctttt agggagggag ctgccgaagg tggggtcggt
     7081 aattgggact aagtcgtaac aaggtagccg tacgggaacg tgcggctgga tcacctcctt
     7141 tctaaggagc atcaaagaaa ccggcttgat tgtccggttt ccagcttcta gttgatctgc
     7201 tggctattcg cttcaaactt cgaagtccac caggggtgga acaagcaagc tcaaaccgtt
     7261 cgcggtggga gaagcttgga tgagctccat gagacttcgg accacagatc gggcacattg
     7321 ttcagttgtg aaggagttca tgacctatcg aggtcgaacg ccttcactgg caacttgaga
     7381 actgcatagc gagcacgttg tcttggattc tctaatatcc aagctactaa gagtcaacgg
     7441 tgaatgcctt ggcgctagaa gccgatgaag gacgtggcag gctgcgaaaa gcctcgggaa
     7501 gctgtcaagc aagcgtcgat ccgaggatgt ccgaatgggg aaacccggct ggggtcatgc
     7561 ccagtcactc tggtctgaat atatagggcc aggtgaggta accaggggaa ctgaaacatc
     7621 tcagtactct gaggaaaaga aagcaaaagc gactccctga gtagtggcga gcgaaacggg
     7681 atcagcctaa acctatgcag tgtaataacc cggtggtgtt gctgtattgg tgtcgtggga
     7741 cactatgtct cggtgcaccg gcatcgagct gaagttacaa agttggcggt tagtcgaacc
     7801 cagatggaaa gctgggccgc agaaggtaac agccctttag acgaaaaccg accaaccttc
     7861 agattggtgt tcccaagtat cacggaggat attccgtgtg aatctgcgag taccacctcg
     7921 taaggctaag tacttcctag cgaccgatag cggacaagta ccgtgaggga aaggtgaaaa
     7981 gaaccccggc gaggggagtg aaatagtacc tgaaaccgtt gacttacaag cagtaggagg
     8041 gatcttcgga tcctgactgc ctgccttttg aagaatgagc cggcgagtta ccggtatgtg
     8101 gcaagattaa ggtgtgaacc gtagtcgaag cgaaagcgag tcttaatagg gcgcctagtc
     8161 gcatgccgta gacccgaagc cgagtgatct atccatgggc agggtgaagc gagggtaaga
     8221 cctcgtgaag gcccgaaccc accaaggttg aaaacttggg ggatgacctg tggatagggg
     8281 tgaaaggcca aacaaactcg gcgatagctg gttctccccg aaatagcttt agggctagcg
     8341 tcgtacgttc cgtattgggg gtagagcact gattggacta gggggcctac aagcttaccg
     8401 aattcagtca aactccgaat ctcaatacgc caagtacggc agtcagacca cgggagataa
     8461 gtttcgtggt cgaaagggaa acagcccaga tcgtcagcta aggcccccaa gtgtgtacta
     8521 agtgggaaac gatgtggagt tgctcaaaca gccagtatgt tggcttagaa gcagccacca
     8581 tttaaagagt gcgtaacagc tcactggtcg agtgattctg cgcggaagat gtaacggggc
     8641 tcaagtacac cgccgaagct acgggatcca gcttttgctg gatcggtagg ggagcgtcgt
     8701 gttgccggtg aagcggcggc ggaagccagc cgtggaggct acacgagtgc gaatgccggc
     8761 atgagtagcg aatgaagagt gagaatctct tccgccgaat gtccaagggt tcctggggaa
     8821 ggttcgtccg cccagggtaa gtcgggacct aaggcgaggc cgagaggcgt agtcgatgga
     8881 cgaccggttg atattccggt accaccgtga gtgcgcccag gccgaatctg gtttgctaag
     8941 gaaagccctt cggggcctac cgacccagct agtactaggt cagcgatgga gtgacgggga
     9001 aggctaaacg gacccgggcg atggttgttc ccggggtaag cgtgtgggcg aggtctaggc
     9061 aaatccggac ctcataaagc cgagacgtga tgccgagccg attcaggcga agccgttgat
     9121 gccatacctc caagaaaagc ttctagcgag cactcaaggt gcccgtaccc caaaccgaca
     9181 caggtggaca ggtagagaat accaaggcga gcgagttaac tctggttaag gaattcggca
     9241 aaatagctcc gtaacttcgg gagaaggagc gccccggtaa ggtgaaggac ttcgcgtcct
     9301 gagcttgagg gggccgcagt gaccagaccc aagcgactgt ttaccaaaaa cacagcagcg
     9361 tgcgaagtcg caagacgatg tatacgctgt gacgcctgcc cggtgctgga aggttaagtg
     9421 gataggttag cttcggcgaa gctttgaaac taagccccag taaacggcgg ccgtaa
//
LOCUS       DBNHXU010000076         1524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3396541, whole genome shotgun sequence.
ACCESSION   DBNHXU010000076 DBNHXU010000000
VERSION     DBNHXU010000076.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3396541"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1070
                     /locus_tag="AC161X_02020"
     CDS             <1..1070
                     /locus_tag="AC161X_02020"
                     /inference="COORDINATES: protein motif:HMM:NF017200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="YlbL family protein"
                     /protein_id="HIR4988356.1"
                     /translation="VQHYVHDLYFAVTVTISTMEDESKRSSSWPLYLVGAFFLMGALA
                     LAAWNIELPYLAFSSGPVSDAGDAITASDVDTFPPSGELLMLTVVSQDVNLFEAFIAG
                     LDPTIDLVPKEAYQPPDETDEQYRQRVLAQMDDSQQRAITVALAYLGYDMVPIDVRIT
                     DLVDDAPSSDVLEIGDSVNSFDGVEIVSGTDLTDALQGHNPGDLVDVGITREGSDKTV
                     EVELAERPDEPGQAMIGITVGELTEPPFPVSIEAGAVGGPSAGMMHTLAIVDTLTAGE
                     LTQGHVIAGTGTIAYDGTVGNIGGIRQKIVGAEAAGAEYVLVPEGNYDEALTAERKAI
                     EIVPVGSLQDAIDFLETLDAI"
     gene            1116..>1524
                     /locus_tag="AC161X_02025"
     CDS             1116..>1524
                     /locus_tag="AC161X_02025"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009987 - cellular process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR4988357.1"
                     /translation="MEPDLAPEEIRRAQFRTVLRGLDGAEVTQYLEKLAGDVETLIGQ
                     RDRLASRLGEYADRDLKSEFDDVGREVATVLQSAREAAESMRERASLDAARWRAESIG
                     EAETLLSDARNDAEALRGDAWATGTELINQTHAE"
BASE COUNT          303 a          476 c          475 g          270 t
ORIGIN      
        1 gtgtgcaaca ttacgtccac gacctctact tcgccgtgac agttacgatt tcaaccatgg
       61 aagacgagag caagcgcagc tcttcctggc ccctctacct ggtaggggcc ttctttctga
      121 tgggcgctct ggccttggcg gcatggaaca tcgaactgcc ctatctcgcc ttttcctccg
      181 ggccggtgtc cgatgccggt gatgccatca cggcgtcgga tgtggatact ttcccaccga
      241 gtggggaatt gctgatgctc accgtcgtca gccaggacgt caatctcttc gaggcattca
      301 ttgctggcct cgatcccacc atcgacctcg ttcccaagga ggcctaccag ccaccagacg
      361 agaccgacga gcagtatcgc cagcgggtgc tcgctcaaat ggacgactcg caacagcggg
      421 cgatcacggt cgctcttgcc tacctgggct atgacatggt ccccatcgac gtccgcatca
      481 ccgacttggt cgacgacgct ccatcatccg acgtgctcga gatcggcgat tcggtgaact
      541 cattcgatgg cgtcgagatc gtcagcggca ccgatctcac cgatgcactt caaggccaca
      601 accccggaga cttggttgac gtcgggatca ccagggaagg gtctgacaag acagttgagg
      661 tcgaactggc tgaacgcccg gatgaaccgg gccaggcgat gatcgggatc actgtcggcg
      721 agttgaccga gccgccgttc ccggtgtcga tcgaagccgg cgccgtcggc gggccttcgg
      781 cggggatgat gcacaccctg gccatcgtcg acaccctgac tgcgggcgaa ttgacccagg
      841 ggcacgtgat tgccggcacc gggaccatcg cctacgacgg cactgtgggg aacatcggag
      901 gcatccgtca aaagatcgtt ggcgctgaag ctgcgggagc cgagtacgtc ttggttcccg
      961 agggcaacta cgacgaggcg ctcaccgcag agcgaaaggc gatcgaaatc gtgccggtcg
     1021 ggtcgctcca ggatgccatc gacttcctcg aaactctcga cgccatctag ccaaagcgcg
     1081 acgaaacacc ggggggtcgg tatctttcga gtctgatgga gcctgacctc gcccccgagg
     1141 agatccgccg cgcacagttt cggaccgtgc tcagaggcct cgatggcgct gaggtcaccc
     1201 agtatctcga gaaactggcg ggcgatgtcg aaaccctgat cgggcaaagg gaccgtctcg
     1261 cgtcccgact cggggagtat gcggaccgcg atctcaagag cgagttcgac gatgtcggcc
     1321 gcgaagttgc cacggtgctc cagtcagccc gagaggcagc ggaatcgatg cgcgagcggg
     1381 caagtctcga cgctgcgcgc tggcgcgccg agtcgatcgg agaggccgag accttgctca
     1441 gcgatgcgag aaacgacgcc gaggcgcttc gtggggacgc ctgggctacc ggcaccgaac
     1501 tgatcaacca gacccacgca gaaa
//
LOCUS       DBNHXU010000077         2751 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3198256, whole genome shotgun sequence.
ACCESSION   DBNHXU010000077 DBNHXU010000000
VERSION     DBNHXU010000077.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2751)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2751)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2751
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3198256"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1463)
                     /locus_tag="AC161X_02030"
     CDS             complement(<1..1463)
                     /locus_tag="AC161X_02030"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR4988358.1"
                     /translation="MEQFEVVWRPSAERLAATHVAKFMELHGLADFESLRRRSIQDPE
                     WFWESTIEYLGLPFDKPWRTILDTSRGTPWATWFAGSLFNLNKACVDRWADQTSDRVA
                     LRSLKETGEKRELNFAELRSEVSALAGALAALGVGRGDAVAVFLPMSTEAVISLLAVA
                     RIGAIFIPIFSGYGAEAVAGRLIDPKPKVLICANGFVRRGSLVEMKEVADRALELAAG
                     VSHVVVVEYAERGTTPMVGGRDLWWKDALGETDPHPVVLTAAEDPFLIAYTSGTTGRP
                     KGAVHVHGGFGTQVAAEGAFQFDFHQDDTVMWVTDMGWIMGPWIIIAGLANGSALATY
                     DGAPDYPGPDRIWQVASELELTFLGVSPTLVRALRIHGAEQAQRHDLSRLRTFGSTGE
                     PWNPEPWWWLFRDIGGESRPIINISGGTEIGAVLLSVNVHQGMKPTSLGGPSLGVDAD
                     VYDNAGKPMRGEVGELVVRGSWPGRTRGFWNEPDRYLA"
     gene            1532..2623
                     /locus_tag="AC161X_02035"
     CDS             1532..2623
                     /locus_tag="AC161X_02035"
                     /inference="COORDINATES: protein motif:HMM:NF013740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HIR4988359.1"
                     /translation="MASSSEKADNRRFRRRTFVVAGVLVATAASVWLIFRLSNIIFMI
                     FVALFVAVALEPPVHFLVKRGWRRGAATGVVFLGGFALVVVFLVALVPLFVEQVNQFV
                     DAAPGYVTSLATFLEDTFGVQLSDLEIAKEGQNLADIIGSAGGTLLGGVIGLTTSLFS
                     SVFFASTVAIFAFYMLAELPKLQHTVLSFMPPEQQKDAMHVWDIAVEKMGGYIYSRLI
                     LAILSGLLSTVVLTFLNVPFALSLGIWVGVLSQFIPIIGTYLAAILPAVVALSANGPT
                     NALWVIIYFIAYQQLENYLVAPRITKRTMEIHPAVSIGAILIGGALLGGLGIILALPM
                     TGIIQALISESSSYRHEVISTVAEPVAET"
BASE COUNT          479 a          878 c          821 g          573 t
ORIGIN      
        1 gcgaggtatc gatcgggttc gttccagaat ccacgcgtcc tacccggcca cgagccacgc
       61 accacaagct ctccgacttc gcctcgcatc ggtttgccag cgttgtcgta aacgtcggca
      121 tcaacgccca gcgatgggcc gccgagtgat gtcggcttca tgccctggtg gacattgacc
      181 gatagcagca cagctccgat ctcggttcct cccgagatgt tgatgatggg cctgctctcc
      241 ccgccaatgt ccctgaacag ccaccaccac ggctccgggt tccatggctc ccctgtggag
      301 ccaaaggtcc gcagcctcga cagatcgtgg cgttgtgctt gttcggcgcc gtggatgcgg
      361 agcgctcgca ccagggtcgg tgagacgccg agaaaggtca gttcgagttc ggaggcaacc
      421 tgccagatcc gatcaggccc cgggtagtcc ggagcaccgt cgtatgtggc gagagccgat
      481 ccgttggcca ggccggcgat gatgatccag gggcccatga tccagcccat gtctgtgacc
      541 cacatcaccg tgtcgtcttg gtggaagtcg aactggaaag cgccctccgc cgccacttgg
      601 gttccgaaac ctccatggac atggaccgcg cctttcgggc gcccggtggt cccggaggta
      661 taggcgatca ggaacgggtc ctcggcagcg gtcaggacca cgggatgggg atcggtctct
      721 cccaaggcat ccttccacca aaggtcgcga ccgcccacca tcggagtagt cccccgctcc
      781 gcgtactcca ccaccacgac gtgactcaca cccgcagcga gctcgagggc ccggtcggcc
      841 acctctttca tctcgaccag gcttccgcgt cggacgaacc cgttggcgca gatcaagacc
      901 ttcggctttg ggtcgatgag ccggccggca acggcttcgg ccccataacc ggagaagatc
      961 gggatgaaga ttgctccgat ccgggccaca gcgagcaacg agatcacggc ctcagtcgac
     1021 atcggcagga acacggccac ggcgtccccc ctccctaccc ccagggccgc cagtgcgcct
     1081 gccagcgcgg acacctcgct gcggagctcg gcgaagttca gctcgcgctt ctcaccagtc
     1141 tccttcagcg atcggagggc gactcgatcc gaggtctggt cggcccaacg gtcgacgcag
     1201 gctttgttga ggttgaacaa cgagccggcg aaccaggttg cccatggagt tcctcgggag
     1261 gtgtcgagga tcgttcgcca tggcttgtcg aacgggaggc cgagatactc gatcgtggac
     1321 tcccagaacc attcgggatc ctggatcgag cggcgtcgca acgactcgaa gtcggccaga
     1381 ccgtgcaact ccatgaactt cgcgacgtgg gtagccgcca accgctcggc gcttgggcgc
     1441 caaacgactt cgaactgctc cattgttctc cttgacctcg tggaggctag gagccatcct
     1501 cgcgaccact cgcggcctac cctgcccgga tatggcctcc tcatcggaga aggcggacaa
     1561 ccgccgcttt cgtcgtcgga cctttgtggt ggcgggagtc ctggtagcga cagccgcctc
     1621 ggtatggctc atctttcggc tgagcaacat catcttcatg atcttcgtcg cgttgttcgt
     1681 agcagtggcg ctggagcctc cggttcactt cctcgtcaaa cgagggtggc ggcggggtgc
     1741 cgcaaccggt gtcgttttcc tcggcgggtt tgccttggtg gtggtctttc tcgtggctct
     1801 cgtgccgttg tttgtcgaac aggtcaatca gtttgtggac gccgcacccg gatacgtgac
     1861 gtcgctggcc acgttcctgg aggacacctt tggggtgcaa ctctctgatc tcgagatcgc
     1921 aaaagaaggg cagaatcttg ccgacatcat cggcagtgct ggtgggactc tgctgggcgg
     1981 ggtcatcggg ctgaccacca gccttttcag ctccgtcttt tttgcgtcca cggtcgccat
     2041 cttcgccttc tacatgctcg ccgagttgcc caagctccag cacactgttc tctcgttcat
     2101 gcccccggag cagcaaaagg atgcgatgca cgtctgggac atcgcggtgg agaagatggg
     2161 cggctacatc tactcccgtc tcatcctggc cattctgagt ggtctgctct cgactgtcgt
     2221 cctcacgttt ctcaacgttc cctttgccct ttcactgggg atttgggtgg gggttctaag
     2281 tcagttcatc ccgattatcg gcacctatct ggcagccatc cttcccgccg tggttgcact
     2341 gtcggccaac gggccgacca acgcgctctg ggtgatcatc tatttcatcg cctaccaaca
     2401 actcgagaat tatctggtgg caccgaggat caccaagcgg acgatggaga tccacccggc
     2461 cgtctcgatc ggtgcgattc tgatcggagg ggcgttgttg ggcggattgg ggatcattct
     2521 ggcgctgccc atgaccggga tcattcaggc gctgatctct gaatcgtcca gctatcgcca
     2581 tgaggtcatc agcacagtcg cagagcctgt ggccgagacc tgaacatccc gatccttggt
     2641 gttagttcgg acctgcttta cctttacccg aacacgggct cactctccgg gccaagggcg
     2701 cctatggcgg acccggaggg caggttccag gactgaatcg atcgagtcaa t
//
LOCUS       DBNHXU010000078         1546 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2602478, whole genome shotgun sequence.
ACCESSION   DBNHXU010000078 DBNHXU010000000
VERSION     DBNHXU010000078.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1546)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1546)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1546
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2602478"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..645
                     /locus_tag="AC161X_02040"
     CDS             <1..645
                     /locus_tag="AC161X_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013159083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4988360.1"
                     /translation="YEWDYARQMMAIRLLEARGEGALATPRDIMSREYLIARPLLQAI
                     ETSVNGHRPVLLIDELDRADEEFEAFLLELLSDFQVTIPEIGTIVADERPIVVLTSNR
                     TREIHDALKRRCIYHWIDYPTPAREADIIKRKVPELEDDLAAAITKAMQELRRMELFK
                     PPGVAETLDWAESLRLLGAGELTIPTIERTLGVVLKYKDDIDLVTGAGIDALVG"
     gene            642..>1546
                     /locus_tag="AC161X_02045"
     CDS             642..>1546
                     /locus_tag="AC161X_02045"
                     /inference="COORDINATES: protein motif:HMM:NF017569.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR4988361.1"
                     /translation="MRKPGLVDQLSEFSEALRNRGLTVTSEQTADMARALTIVDLARR
                     SQAQAALRSLTVTNPSQIPVFNEEFQIFFDRALPRLTTRKPNAPPTQATASYHLETAG
                     IGGDTDVVDQGGASAAERLATRDFSDLDDEELGEARRLVMSMMWHPSPYQTRRWVVGD
                     GRRPDLRRTLHRAVGPTGDLMEMQWKRRRKRERPLIIIADVSGSMDKYAELFLVFAHA
                     AQRRLREVEVFTFSTELTRITEDLARRDTRSALARVSGRVHDWSGGTKIGEALGTWNR
                     IWSRRLARGGPVVLILSDGWDCGDPT"
BASE COUNT          290 a          512 c          474 g          270 t
ORIGIN      
        1 tacgaatggg actacgcccg ccagatgatg gcaatccgcc tcctcgaggc gcgcggagaa
       61 ggggccctcg ccacccctcg cgacatcatg tcccgcgagt acctgatagc ccggcctctc
      121 cttcaggcca tcgagacgag tgtgaatggg caccggccgg tgctgctcat cgatgagttg
      181 gatcgcgccg acgaggagtt cgaagccttc ctcctcgaat tgctctctga cttccaggtc
      241 accatccccg agatcgggac catcgtcgcc gacgagcgcc cgattgtggt tctgacctcc
      301 aaccggacgc gggagatcca cgatgcgctc aagcgtcgct gcatctatca ctggatcgac
      361 tacccaaccc cggcccggga agcggacatc atcaagcgaa aagtgcccga gctggaggac
      421 gacctagctg ccgcgatcac caaggcgatg caggagcttc gtcggatgga gctcttcaaa
      481 ccgccgggag tggccgagac cctggactgg gcggagtcgc ttcgccttct cggcgccggc
      541 gaattgacga tcccgaccat cgaacgcacc ctcggagtgg tcctcaagta caaggacgac
      601 atcgacctgg tgactggagc gggaatagac gctctcgtgg gatgagaaag cccggtctcg
      661 tcgatcagct ctcagagttc tccgaggccc tccgcaatcg tgggctcacc gtcacctccg
      721 agcagaccgc agacatggcg cgggctctca ccatcgtcga tctcgcccgg cgctcccagg
      781 ctcaggccgc tctccgttcg ttgacagtca ccaatcccag ccagatccct gtgttcaacg
      841 aggaattcca aatcttcttc gaccgtgccc tgccccggct aaccactcga aagccgaatg
      901 cgcctcccac ccaggccaca gcctcctacc acttggagac cgcgggaatc ggcggtgaca
      961 ccgacgtggt cgaccagggt ggggcatctg ccgcggagcg cttggcgacc cgggacttct
     1021 ccgacctcga cgatgaggaa ctgggcgaag ctcgtcgcct ggtgatgtcg atgatgtggc
     1081 acccctcgcc ctaccagaca cgacggtggg ttgtcgggga tgggcgtcgt cccgacctgc
     1141 gccgcacgct tcatcgagca gtcggcccaa caggcgatct catggagatg caatggaaac
     1201 ggcgtcgcaa gcgtgagcgc ccgctgatca tcatcgccga tgtctccggg tcgatggaca
     1261 agtacgccga gctgtttctg gtgttcgctc atgctgccca gcgacgtctc agagaggtcg
     1321 aggtcttcac cttctccacg gagctgaccc ggatcaccga ggacctggcg agacgcgaca
     1381 ccagatcggc gttggctcga gtctccggac gggtccatga ctggtcaggc ggcaccaaga
     1441 tcggcgaggc cctgggaacc tggaaccgga tctggagtag gcgcctggcc aggggcggcc
     1501 cggtcgtgct catcctctca gacgggtggg actgtggtga tccgac
//
LOCUS       DBNHXU010000079         4252 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3397462, whole genome shotgun sequence.
ACCESSION   DBNHXU010000079 DBNHXU010000000
VERSION     DBNHXU010000079.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4252)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4252)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4252
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3397462"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC161X_02050"
     CDS             <1..348
                     /locus_tag="AC161X_02050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988362.1"
                     /translation="SSTIDDDDVNDDRGEDDADQDDDNKGHGGEDDDSAVAVADGVHT
                     YQVPGAGSVTINVTGGNLELADVQANTGWTTEVDKAQSDRIKIKFRNGESEAEFEAEL
                     EHGAITIDIEQGD"
     gene            complement(356..1627)
                     /locus_tag="AC161X_02055"
     CDS             complement(356..1627)
                     /locus_tag="AC161X_02055"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HIR4988363.1"
                     /translation="MRRQLGLWSLAITTLVGVSLTIPLALLVQRQAAERAQVEAELST
                     QATASLIALASALTGEVDPVAVADSVGPLPPGQIVILPDGTVIGDPGEFPRTLTTEVA
                     ETGRSAADYVEGGWELAVPVVVREGTVVISTFVSDDELTEGVWQAWLLLGLLGVVTVA
                     AALAVADRLAKRLVEPLTSLADTATRLGEGDLTARVEVSPPREIEELGRTFNWLADRL
                     DQLLAEEREAVADLSHRLRTPLTSLRLQAEGIADGEERAALVAQVGRVEAAVDQLIHE
                     ARRDRYKAPGKCDLGLVVSNRAAFWRILAEEQHREFQVDISAGNLEVGLGEDALAALV
                     DTLMENVFSHTPEGTGFGIRVSAASRPILEVFDHGPGFPSSEAVARGVSGGGSTGLGL
                     DIAKRTAELSGGGLELSDRPGGGAVVRVWFG"
     gene            complement(1624..2307)
                     /locus_tag="AC161X_02060"
     CDS             complement(1624..2307)
                     /locus_tag="AC161X_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013731845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR4988364.1"
                     /translation="MARIILIEDDIEIRRLVAGALAREGHDVETAATAMSGLELAIRN
                     TPDLVIMDLGLPDLDGTELLRMIRAISSVPVMVITARGADDAVVHTLDAGADDYLVKP
                     FSVAQLLARVRALLRRGSHVEGSDRVVVGGLVIDISAREASLEGAALDLSPKEFDLLR
                     SLAERQGEVVTKRELLAEVWREPYGGSERTVDVHLSWLRKKLGETASEPRFLRTVHGV
                     GVKLVPPES"
     gene            complement(2378..2677)
                     /locus_tag="AC161X_02065"
     CDS             complement(2378..2677)
                     /locus_tag="AC161X_02065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988365.1"
                     /translation="MAATLVDVAPGDDAVVTVVESETGRVVGVLDVDVDESGRVVEDD
                     ETTGAAMGVNGPGLTCSPAAATICQARTVVRVVAATQIVMRPYRFMTRFSQQKAG"
     gene            2667..2915
                     /locus_tag="AC161X_02070"
     CDS             2667..2915
                     /locus_tag="AC161X_02070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988366.1"
                     /translation="MAATWQVKTIPTGGGTVVVSYRPGEVVLDGATPAAGFTVEVDKQ
                     GPPEVRVKFESESSKVEVRVDWDGKLVTDVQVDGEDDD"
     gene            complement(2916..3365)
                     /locus_tag="AC161X_02075"
     CDS             complement(2916..3365)
                     /locus_tag="AC161X_02075"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR4988367.1"
                     /translation="MWRSALVTKKEKLEKLGSVPLFQGLSQKDLEKILDRMKETFHVA
                     GVSVIDEGRAGIGFHLILEGEARVLRNGRVAARLGPGEFFGELALVDDGPRSATVAAV
                     TDLTTVVLSKWEFRPLLKDHPEMAWKLIEYLVARVREEQTGRDALVC"
     gene            complement(3420..4136)
                     /locus_tag="AC161X_02080"
     CDS             complement(3420..4136)
                     /locus_tag="AC161X_02080"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase-related protein"
                     /protein_id="HIR4988368.1"
                     /translation="MRIGLLVCDHVRPEFLDISGDYEDMFRRLFDNHNDIEVVGYDAI
                     NGELPGDPSEADAWLTTGSRHSVNDDEPWIRDLEEFVRHVAESGVPFIGICFGHQLIA
                     KALGGSVVKSDRGWGIGLQEVELSEDLGWGESYRLLTSYQDQVDSLPPGAEILGWNDH
                     CPVSVMGVGSNILGLQGHPEFESAYSQALMESRRGTLIPEATVDAGLASLEHEPDGSK
                     LADWILEFVAAAQKASAADS"
BASE COUNT          862 a         1378 c         1250 g          762 t
ORIGIN      
        1 tcctccacca tcgatgacga tgacgtcaac gatgaccggg gcgaggacga tgccgaccaa
       61 gacgatgaca acaagggcca cggcggcgag gacgatgact ctgctgtcgc agtcgcagat
      121 ggagtgcaca cctatcaggt tcccggcgcg gggagcgtca ccatcaacgt cacgggcggg
      181 aacctcgaat tggcggacgt gcaggccaac accgggtgga ccaccgaggt agacaaggcc
      241 caatcggacc ggatcaagat caagtttcgc aacggtgaga gcgaggccga attcgaagcc
      301 gagctcgagc atggcgcaat cacaatcgac atcgagcagg gcgactgacc tctccctaac
      361 cgaaccagac tctgacgacc gccccgccac cggggcggtc gctcaattcc aggccgccac
      421 cactcagttc ggctgtgcgc ttggcgatat cgagcccaag gccggttgat cccccaccac
      481 tgacgcccct ggcgaccgcc tccgacgatg ggaaacctgg gccatggtcg aaaacctcga
      541 gaatgggcct tgaagccgca ctgacacgga tgccgaagcc ggtgccttcg ggggtgtggc
      601 tgaacacgtt ctccatgaga gtgtccacca gcgcagcgag agcgtcctcc ccgagcccca
      661 cttccagatt gcctgccgag atatcgacct ggaattcacg gtgctgctcc tcggcgagga
      721 ttcgccagaa ggctgcccgg ttggaaacaa ccaacccgag atcgcacttt cctggcgcct
      781 tgtagcggtc ccttctggcc tcgtggatca attgatcaac ggccgcttcg acgcgaccga
      841 cttgagccac cagagcggcg cgttcctcac cgtcggcgat cccctcggcc tgaagtcgaa
      901 ggctggtgag cggggttcgc aatctgtggg acagatcggc gacggcctcc cgctcttccg
      961 ccagcaattg atcgagacga tcggccagcc agttgaaggt gcgacccaat tcctcgattt
     1021 cgcggggcgg gctgacctcg actctggcgg tcaagtcgcc ctcccccagt ctcgttgccg
     1081 tgtcggccag tgaggtcagt ggttccacga gacgcttcgc caaccgatct gcgacggcga
     1141 gcgctgcagc gactgtgacc actcccagga ggcccagaag cagccacgcc tgccacaccc
     1201 cttcggtcaa ctcgtcatcg ctgacgaaag tcgagatgac gaccgttcct tcacgtacca
     1261 ccacagggac tgccagctcc cacccaccct cgacgtagtc ggccgcagat cgaccggttt
     1321 cggcgacctc cgtggtcagc gttcgtggga actcacccgg atcgccgatc accgtcccgt
     1381 caggaagaat cacgatctga cctggcggca gcggaccgac agagtcagca accgcaaccg
     1441 gatcgacttc cccagtgagg gctgaagcca gtgcaatcag cgatgcggtg gcctgggtgc
     1501 tcaactcggc ttccacctgt gcccgctcgg ctgcctggcg ttgcacgagg agggcgagtg
     1561 ggatcgtcag cgagacacca accagggtcg tgatcgcaag actccacaga cccagttgtc
     1621 tcctcatgac tcaggtggga cgagcttgac ccccacccca tgaaccgtcc tgagaaacct
     1681 gggttcggag gcggtttccc ccagtttctt tcgcagccag gagagatgga catctacggt
     1741 tcgctcgctc cccccgtatg gctctcgcca gacctccgcg agcagctctc gtttggtgac
     1801 cacctcgccc tgccgctcgg ccaatgaacg cagcagatcg aattctttgg ggctcaaatc
     1861 aagagcggcg ccttccaacg atgcttcgcg tgcagagatg tcgatcacca accccccgac
     1921 gacgacgcga tcgctcccct cgacatggct tccccggcgc aggagagctc tgactcgtgc
     1981 aaggagttgg gcaacggaga agggcttgac caggtagtca tctgcgccgg cgtcgagagt
     2041 gtgaacgacg gcgtcatccg caccgcgggc ggtgatcacc atcacgggga ccgagctgat
     2101 cgcccggatc atgcggagaa gctcggtgcc gtcgagatcg gggagcccga gatccatgat
     2161 cacgagatct ggtgtgtttc tgatcgcaag ctccaagcca ctcatcgccg tagcagcggt
     2221 ctccacgtcg tggccctccc tggccagggc gccggcgacg agtcttcgga tctcaatgtc
     2281 gtcctcgatg aggatgattc gagccatgcg accagggtat ctgtgatcac ccccgctagc
     2341 tcacgccttg agaaaacctt gataatgcgt tgatctgtta gccagccttc tgctgggaga
     2401 atcgtgtcat gaatcgatat ggcctcatca cgatctgggt ggcggccacc accctgacga
     2461 ctgtccttgc ctggcagatc gtcgccgcgg caggcgagca agtcagtcca ggaccgttga
     2521 cccccatcgc tgcgcccgtg gtctcatcgt cctcaaccac cctgcccgac tcatccacgt
     2581 cgacatccaa cactcctacc actcgacccg tttccgactc gaccaccgtt accacggcat
     2641 cgtctcccgg agcaacgtcg acaagcgtgg ccgccacctg gcaggtgaag acgatcccga
     2701 ccgggggcgg gactgtggtg gtcagctacc gaccgggtga ggtagtcctc gatggcgcaa
     2761 cgccggcagc cggttttacg gtcgaggtcg ataaacaggg tccgcccgaa gtcagagtca
     2821 agtttgaaag cgagtcgtca aaggtcgagg ttcgagtcga ctgggatggc aaattggtga
     2881 ccgatgtcca ggtggacgga gaagacgacg actgatcagc agaccagggc gtctcttcct
     2941 gtctgctctt cacgaaccct ggcaaccaag tactcgatca gtttccatgc catctcgggg
     3001 tggtccttga gcaaaggtcg gaactcccac ttggagagga cgacggtggt caggtccgtt
     3061 accgcagcca cggtggctga tcgagggcca tcgtcaacga gggcaagttc accaaagaac
     3121 tctcccgggc caagacgggc cgcgacccgc ccgttgcgga gcacacgggc ctcaccttcg
     3181 aggatcagat gaaacccgat cccagcgcga ccttcgtcga tcacgctcac gccggcgacg
     3241 tggaacgtct ccttcattcg gtccaagatc ttctcgaggt ccttctgact cagaccttgg
     3301 aacagaggga ctgagccgag cttttcgagc ttctccttct tggtgaccaa ggcactcctc
     3361 cacatcgttc agcggcgctc ggatcatatc tgtcgaaggc ctgtcgcgcc ctcagcggct
     3421 caggaatcgg cggctgaggc tttctgtgcc gccgccacga actcgaggat ccaatcagcc
     3481 aatttcgagc cgtcgggctc gtgctccagc gaggccagac cagcatccac agtcgcctcg
     3541 gggatcaagg ttccgcgtcg agactccatg agcgcctgcg agtaggcgga ctcgaactct
     3601 gggtgacctt ggagcccgag gatgttcgat ccgactccca tcacggacac cggacaatga
     3661 tcgttccagc caaggatctc ggcgcccggt ggcagcgaat cgacctgatc ctgataactg
     3721 gtgagaagcc gataagactc gccccaaccc agatcctcgc tgagctcgac ctcttggagg
     3781 cctatgcccc aaccacgatc cgacttcacc accgagccgc caagggcttt ggcgatgagc
     3841 tgatgcccga aacaaatgcc gatgaagggc acaccggatt cagccacgtg gcgaacaaac
     3901 tcttcgagat cccggatcca gggctcgtca tcattcaccg agtgtcgaga gccggttgtc
     3961 agccaggcgt ccgcctcgga ggggtcgccg ggcagttctc cgttgatagc gtcgtagccc
     4021 acgacctcga tgtcgttgtg attgtcgaat aggcgacgaa acatgtcctc gtagtccccg
     4081 gagatgtcga ggaactcggg ccgaacatgg tcacaaacga gaagaccgat tctcatgcga
     4141 cccccattct ggcgcggaga tctcggcatg caggcccgga tatccgcgcc aaaacaggag
     4201 gttggagacc gatcaatcgc tggcgaccga tttggcgcgg agatctggac ct
//
LOCUS       DBNHXU010000080         3305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_619381, whole genome shotgun sequence.
ACCESSION   DBNHXU010000080 DBNHXU010000000
VERSION     DBNHXU010000080.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_619381"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(62..817)
                     /gene="sufC"
                     /locus_tag="AC161X_02085"
     CDS             complement(62..817)
                     /gene="sufC"
                     /locus_tag="AC161X_02085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006447382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HIR4988369.1"
                     /translation="MSDTAPLLQIENLHASAKDVEILRGVDLTVDHGEIHALMGPNGS
                     GKSTLASVLMGHPGYKVTKGRILYRGEDITATPSYQRGAAGMFLAFQHPEAIPGVSVI
                     QFLRQALSNRTGVDLTVLELRLKVMEAMKELGMESSFADRYLNEGFSGGERKRNEVLQ
                     MAVLEPELAIMDETDSGLDIDALRTVADGVNKLTGPERGFLIITHYQRLLDYVTPDQV
                     HIFMDGQVLESGGPELAARLEEEGYDSFRSVLT"
     gene            complement(875..1336)
                     /locus_tag="AC161X_02090"
     CDS             complement(875..1336)
                     /locus_tag="AC161X_02090"
                     /inference="COORDINATES: protein motif:HMM:NF015999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HIR4988370.1"
                     /translation="MASHQDFAEHVKLFPEGTKTAEDAAAAVGCPVAAIVKSLVFVVI
                     DASGGEEPVVALVPGDLRLDTDALASCAGARESRRASLDEVRAATGYAAGGTPPFGHV
                     TPVRVFADPALRRHDPVWAAAGTPTTVFPISIEDLDRLAKPMWAEIAIRSA"
     gene            complement(1366..2280)
                     /locus_tag="AC161X_02095"
     CDS             complement(1366..2280)
                     /locus_tag="AC161X_02095"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR4988371.1"
                     /translation="MKFSIMTEPHLGGTYDQLLAAARLAEAKNLVSFARCDHFLTKRE
                     PTPDATDAFATLAGLARETSTVRLCVLVTPITFRHPAVIAKNAATIDQMSGGRFDLGV
                     GTGWMDFEHASLGISFPGSSERWARFEEALDYLAAAFGPGRSSFEGQYYRIDAEVNPK
                     PAGLRLVIGGSGMNRTPRLAGSRADEYNFFTCSPDEARAKIEVMRETAGDRQVEATVM
                     GPAVVGATEADYRRRLDAFATRFGGGLSPDEVEKEWESSGEIFGVPDQAAERVAALEA
                     AGVERIYLQWLDLADYDGLAETVELVSG"
     gene            complement(2277..>3305)
                     /locus_tag="AC161X_02100"
     CDS             complement(2277..>3305)
                     /locus_tag="AC161X_02100"
                     /inference="COORDINATES: protein motif:HMM:NF014234.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0033730 - arogenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - L-tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HIR4988372.1"
                     /translation="VMPQAAIIGTGLIGASIGLALREQGWATKGWDPSVEALAVASER
                     GAVDTAASIQSAIEAAELVILSGPLSANLDTLGHLETDALVTDVTSVKQPIVHGVPPR
                     LRFVGGHPMAGREHAGPVAASGSLFRGAAWILCDDQASADDLTTMRDVVASIGANPVV
                     MSADHHDRVVAAISHLPHLLAVALVDLVAGNPDNADLVSGSFRDLTRVASAESAWWPE
                     VLAANSESVGEALDSLIGNLEALRADIDTGNLDAVAQSLDRARVMRAEMAPPVAGVEV
                     ILSDRPGEIGLVGQALRTSRVDVRDLQLRHAVHGGGGILTISVRPGEVEPLKAALTRE
                     GFQVEEQA"
BASE COUNT          595 a         1073 c         1060 g          577 t
ORIGIN      
        1 aagtggactc catcaccgag gacgggaaag tcggctttga gatgcgatag atcgatcaca
       61 actaggtcaa cacactccgg aacgaatcgt atccctcctc ttcgagacgg gccgccaact
      121 caggcccccc gctctcgagg acctgtccgt ccatgaagat atggacctgg tccggggtca
      181 cgtagtccaa gagccgctgg tagtgggtga tgatcaagaa cccccgctcc ggcccggtca
      241 gcttgttgac gccgtcggcc accgtgcgca aagcatcgat gtcgagccct gagtcggtct
      301 cgtccatgat cgccaactcc ggctcgagca cggccatctg caggacttcg ttgcgcttcc
      361 gctccccacc ggagaaccct tcgttgaggt agcggtcggc aaaggacgat tccatgccca
      421 gctccttcat ggcctccatc accttgagcc ggagctccaa caccgtgagg tcgaccccgg
      481 tgcgattgga caaggcctga cggaggaact ggatcactga gacgccgggg atggcctccg
      541 ggtgctggaa ggcaagaaac atcccggcgg cgccgcgttg ataggagggg gtggcggtga
      601 tgtcctcgcc gcgatagagg atccggcctt tggtgacctt gtagccggga tggcccatga
      661 gcaccgaggc caatgtcgac ttgcccgatc cgttggggcc catcaaggcg tggatctcac
      721 cgtgatctac ggtcagatcg actccgcgca ggatctccac gtctttcgcc gaggcatgca
      781 aattctcgat ttggaggagg ggggcggtgt cgctcacgag ctcaatggta tttgcactat
      841 gggtgtaggg gaaactcgta ctgcgtgata ggtactacgc actacgtatc gcgatctcgg
      901 cccacattgg tttggccaat cggtccaggt cttcgataga gatcgggaaa accgtggtcg
      961 gggtcccggc tgcagcccac accgggtcgt gtcggcgaag ggccgggtcg gcgaacaccc
     1021 ggaccggcgt cacatgtccg aatggaggcg tgcctccggc tgcgtaaccg gttgccgccc
     1081 ggacttcatc caatgaggcc cgacgtgact ccctggctcc tgcgcaggag gcgagggcgt
     1141 cggtatccag gcggagatcg ccgggcacga gagccacaac aggttcctca ccgcccgacg
     1201 cgtcgatcac cacgaacacg agagacttca cgatcgccgc caccgggcac ccgacggcgg
     1261 ccgcagcgtc ctcggcggtc ttggttccct cggggaacaa cttcacgtgc tcagcgaagt
     1321 cctggtggct cgccatcagg cgttgactcg cctcgggcag atcaatcaac cgctgaccag
     1381 ctctacggtt tcggccagcc cgtcgtagtc ggcgaggtcg agccattgca gatagatccg
     1441 ctcgacgcct gccgcttcga gggcggccac tctctcggcg gcctggtcgg ggaccccgaa
     1501 gatctcaccc gagctctccc actccttctc cacctcgtcg gggctcaagc caccaccgaa
     1561 gcgagtggca aaggcatcga ggcgacgtcg gtagtcggcc tcggttgccc ccacgaccgc
     1621 aggtcccatc acggttgcct cgacctggcg atcgccggcc gtctccctca taacctcgat
     1681 cttggctcgg gcttcgtcgg gggagcaggt gaagaagttg tactcatcgg cccgggagcc
     1741 ggcgaggcga ggggtccggt tcatcccgct tccgccgatc acgagccgga gacctgctgg
     1801 cttcgggttc acctcggcgt cgatccggta gtactggccc tcgaaactcg accgacccgg
     1861 gccgaaggcg gccgccagat agtcgagggc ttcttcgaag cgagcccaac gctcggagga
     1921 cccgggaaag gagatgccga gggatgcgtg ctcgaaatcc atccatccgg tgcctacgcc
     1981 gaggtcaaaa cgacctccag acatctgatc gatggtggcc gcgttcttgg cgatcacagc
     2041 cggatggcgg aatgtgatcg gcgtcaccag cacgcacagt cgaacggtgg aggtctcccg
     2101 ggcgagcccg gcgagcgttg caaaggcatc cgtcgcgtcc ggggttggct ctcgcttggt
     2161 caggaagtgg tcacagcgcg caaaggaaac aaggtttttg gcctccgcga gccgagccgc
     2221 ggctaacaac tggtcgtagg tcccgcccag gtgaggctcg gtcattatcg agaacttcat
     2281 gcttgctcct ctacctggaa tccttcccgt gtcagggcag ccttgagtgg ctcaacctca
     2341 ccgggacgca ccgagatggt gagaatgccg cctcccccgt gcaccgcatg gcggagctgt
     2401 aggtcgcgga cgtcgacccg gctggtccga agggcttggc cgaccagccc gatctctccc
     2461 ggtcggtcgg aaagtatcac ttccaccccg gcgaccggag gtgccatctc tgccctcatc
     2521 accctcgccc gatcgagact ttgggccacc gcgtcgaggt tcccggtgtc gatgtcggcg
     2581 cggagtgcct ccagattccc gatcaagctg tccagggcct ctcctacgga ctccgaattc
     2641 gctgccagca cctcgggcca ccaggccgac tcggcggagg ccacccgggt caggtcccgg
     2701 aaactgccag aaacgaggtc ggcgttgtcc ggattcccgg caaccaagtc gacgagggcg
     2761 actgcgagca ggtgggggag gtgggagatc gcggcgacga ctcgatcgtg atggtcggca
     2821 gacatgacga ccgggttggc cccaatcgat gccacgacat cccgcatcgt tgtcaggtcg
     2881 tcggcactcg cctggtcatc gcaaaggatc caggcggcgc cacggaacaa cgaaccggag
     2941 gccgcgaccg ggccggcatg ctccctaccc gccatcgggt gcccgccgac gaaacgaagg
     3001 cgtggcggca ctccgtgcac gatcggctgc ttgacgctgg tcacgtcggt gacgagggca
     3061 tcggtttcca gatgacccaa cgtgtccagg ttggccgaga gagggcctga gaggatcact
     3121 agttctgcgg cctcgatcgc ggactgaata gacgccgcgg tgtcgacagc gcctcgctcg
     3181 gaggccacgg ccagggcctc gacggacggg tcccatcctt tcgtcgccca tccctgctcg
     3241 cggagggcaa ggccgatgga ggctccgatc agcccggtcc cgatgatcgc tgcctgtggc
     3301 atgac
//
LOCUS       DBNHXU010000081         2837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_422470, whole genome shotgun sequence.
ACCESSION   DBNHXU010000081 DBNHXU010000000
VERSION     DBNHXU010000081.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2837
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_422470"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="AC161X_02105"
     CDS             <1..469
                     /locus_tag="AC161X_02105"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988373.1"
                     /translation="TGTIVIGEGTGRWSVAMVQGLYIMLATYLIFRVNWGDPLGALLL
                     LITFAAVGAGAGMLMGAVFSNDAQATGVGIVVSLGLAALGGCMLPVELFSPAMQKVAH
                     LTPHAWALDGFAELVRRNGTVVDILPQLAILSAYAVVLLLLASWRLRITLTRT"
     gene            487..1002
                     /locus_tag="AC161X_02110"
     CDS             487..1002
                     /locus_tag="AC161X_02110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988374.1"
                     /translation="MADGEQSSTAILGRILSWIGYLWLVFAVLWGLGVAEAIGISGSF
                     ASSIGRSVFPAIILIGVGRVMRKRSKADPNQPQPDVSRPPGPTPPILPEDRPVSSYPP
                     PSPPAPRAPKPMARPASQTKQSAVPSAESLTDSGQSDQQPPPDAGVPLPKHKSSQELI
                     DEARQRWGSRP"
     gene            complement(1011..>2837)
                     /locus_tag="AC161X_02115"
     CDS             complement(1011..>2837)
                     /locus_tag="AC161X_02115"
                     /inference="COORDINATES: protein motif:HMM:NF039580.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR4988375.1"
                     /translation="DPLASYVTRRAWSVLDRAERKLRGNRYKIPRFVPEAILSRKEFL
                     DAVDQASLESRIDLQTGRTNAERYLREIAATHSPYVIDLIANAIHKLYSQGYGAIRYD
                     EDQVRMLAELGQDHPVIFLPSHRSNLDRLSLQFMLWENDMPPNHTAGGINMNFFPVGP
                     MMRRTGVFFIRRSFKDNRLYKTVLKGYLDYLIEKRFPLEWYLEGGRSRSGRLGAPHFG
                     LLSYVVDSLRRGKTDDIELIPVSIAYDQIQDVGDYALEAQGMGKQKESLGWLFRAVKS
                     LRRRYGDIHIRFGEPVSMAAGLASIDPQDEASIGLQKLAFEVMYRIGQVTPITPTALV
                     TIALLAARGNARNAEQLSEDCKGLSEFVTARRLPATEDLDLSDPDRVTTILDWLAEHK
                     NVSSHSAVGRRVFWLDEEQMVRIAYYRNVIVHFFVPRALAEMTLNALADSGEATGPDF
                     DETILKLRDLLKFEFFFQEKLEFLESVSVDMAVDVPNWRASLSSTGIDSILQQMAAPV
                     AQWAVLPFLDAYQVVGDELENSHGAFNEKAFLKACLDRARMYRIEGRVISGESASQVM
                     FKSALSLADNRGLLGRDGDVVNRRRLFAEEIRETRRLAAAGL"
BASE COUNT          507 a          859 c          902 g          569 t
ORIGIN      
        1 gaccggcacc atcgtgatcg gggaggggac cgggcggtgg tcggtggcga tggtccaggg
       61 cctctacatc atgctggcga cctacctgat cttccgggtc aattggggcg acccgttggg
      121 cgccctgttg ttgttgatca ccttcgccgc ggtcggcgct ggagccggga tgctgatggg
      181 agcagtgttc agcaacgatg cccaggccac tggggtcggc atcgtcgtca gtctgggttt
      241 ggctgctctc gggggttgca tgctgccggt cgagttgttc tcgccagcca tgcagaaggt
      301 cgcccatctc actccgcacg catgggccct ggatgggttc gccgagttgg tccgacggaa
      361 tgggacggtt gtcgacatcc tccctcagtt ggcgatcctg agtgcatacg cggtcgtgct
      421 gctgctgctc gcctcgtggc ggcttcgcat cacattgacc cggacctgac tgtttgaatg
      481 ggcgccatgg ccgacggtga gcagagctcc accgccatcc tgggcaggat tctgtcctgg
      541 attggatatc tctggctcgt ttttgcagtg ctctgggggc tcggcgtggc cgaagccatt
      601 gggatatcag gttcgttcgc ttccagcatc gggcgatccg tgttccctgc gatcattctg
      661 atcggggttg gtcgagtaat gcggaagcgg tcgaaggccg atccgaatca gcctcagccc
      721 gacgtttctc gaccgccagg accgacgcct ccgatcctgc ccgaagaccg ccccgtgtcg
      781 tcctatccgc cgccgtcgcc gcctgccccg cgggctccga agcccatggc tcggcccgcc
      841 tcacaaacca agcagtcagc ggtgccttca gcggagtccc taaccgacag tgggcaaagt
      901 gaccagcagc ctccccccga tgccggtgtg ccgttgccga agcacaagag ctctcaggag
      961 ttgatcgatg aggcgcggca gcgctgggga tcgcggccct aatcgcgggc tcagagaccc
     1021 gcagccgcca gacgcctggt ctctctgatt tcctcggcaa atagccgccg ccggttcacg
     1081 acgtctccgt cgcggccgag gaggccccgg ttgtcggcga gtgatagcgc cgatttgaac
     1141 atgacctgac tggcgctctc acctgagatg acccggcctt ctattcggta catacgcgct
     1201 cggtcgaggc aggccttgag aaatgccttc tcgttgaagg caccgtggct gttctccaac
     1261 tcatcgccaa ccacctggta ggcatcaagg aaaggcagca ccgcccattg ggcaaccggt
     1321 gccgccatct gctgcagaat cgagtcaatc ccggtcgacg acagactcgc ccgccagttg
     1381 ggaacgtcga ccgccatgtc gacggagacc gattcgagga attcgagctt ttcttggaag
     1441 aagaactcga acttcaagag gtcgcgaagt ttgaggatgg tctcgtcgaa gtcagggccg
     1501 gtggcctcgc ccgagtcggc gagggcgttg agggtcatct cggccagcgc cctcggcacg
     1561 aagaaatgga cgatcacgtt gcggtaatag gcgatcctga ccatctgctc ttcatcgagc
     1621 caaaagaccc gccgaccgac tgccgagtga ctcgagacgt tcttgtgctc ggccagccaa
     1681 tcgaggatcg tggtcaccct gtcaggatcg gaaaggtcga gatcctcggt ggcgggcaat
     1741 ctcctggccg tcacgaactc agagagcccc ttgcaatcct cggacagctg ttcggcgttc
     1801 ctggcgttgc cccgcgccgc gaggagggcg atggtgacca aagcggttgg agtgatcggg
     1861 gtcacctgcc cgatccggta cataacctcg aatgcgagtt tctgcagtcc gatcgaagcc
     1921 tcgtcctgtg gatcgatcga tgccagcccg gcggccatcg agaccggctc gccgaatcgg
     1981 atatggatgt cgccgtaacg ccggcgcagc gatttgaccg cccggaacaa ccaccccagc
     2041 gactccttct gcttgcccat gccttgtgcc tcgagggcgt agtcgccaac gtcttggatc
     2101 tggtcgtagg cgatcgagac cgggatgagc tcgatgtcgt cggtcttccc acgccgcagc
     2161 gaatcgacca cgtagctgag caagccgaag tggggagcac cgagccgacc cgaccgggag
     2221 cgaccccctt ccaggtacca ctccagaggg aacctcttct cgatgagata gtcgaggtac
     2281 cccttgagaa cagtcttgta gaggcggttg tccttgaagc tcctccggat gaagaacacc
     2341 ccggtgcgcc gcatcatcgg gccgactggg aagaagttca tgttgatccc gccggccgtg
     2401 tgattcgggg gcatgtcatt ttcccagagc atgaattgaa ggctgagccg atcgagattc
     2461 gatcggtgcg aggggaggaa gatgaccggg tgatcctgtc cgagctcggc gagcatgcga
     2521 acctgatcct cgtcgtagcg aatggctccg tatccctggc tgtagagctt gtggatcgcg
     2581 ttggcgatca ggtcgatcac atatggcgag tgagtggcag caatctccct cagatatcgc
     2641 tcggcgttgg tccggccggt ttggagatcg atgcgggatt ccagagaggc ttggtcgaca
     2701 gcatcgagaa actccttgcg agagaggatt gcctcgggca cgaatcgggg gatcttgtag
     2761 cgattccctc gcagctttcg ctcagcccga tcgaggacgc tccaagctcg tctcgtgacg
     2821 tagctggcaa gcgggtc
//
LOCUS       DBNHXU010000082         7417 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_422488, whole genome shotgun sequence.
ACCESSION   DBNHXU010000082 DBNHXU010000000
VERSION     DBNHXU010000082.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7417)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7417)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7417
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_422488"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(77..733)
                     /locus_tag="AC161X_02120"
     CDS             complement(77..733)
                     /locus_tag="AC161X_02120"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF013308.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070567 - cytidylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IspD/TarI family cytidylyltransferase"
                     /protein_id="HIR4988376.1"
                     /translation="MSGRAMIIVAGGTSSRFGGDKLMAQIAGQPLLAHTVAAVAPLVD
                     VCVLVCREEHATEMSKVAPNLILVRGGVTRTRSELAGLGAVPDSIDIIGIHDGARPLA
                     SKSLVNRLFEVAAETGGAVPVLATSNPLVRRSDLRPVSDTAIAQTPQVFLGPELQSAY
                     RAAAAAGFEGHDTAAVVKEFSELTIVAVPGETGNIKVTYPADLDAVRAVLEPSRSELR
                     "
     gene            758..1099
                     /locus_tag="AC161X_02125"
     CDS             758..1099
                     /locus_tag="AC161X_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988377.1"
                     /translation="MIDIRALRKLTEGGKDHLDIDHAGALVDRGELNVEGPAVLFVVP
                     VTDAVKVVVDDLVLDSLDRDDLWSIQGFRLEHEVLLVLQDEVSDPQSLVEAVAAAGFV
                     WQVIHPTTGVL"
     gene            1130..1681
                     /locus_tag="AC161X_02130"
     CDS             1130..1681
                     /locus_tag="AC161X_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988378.1"
                     /translation="MEGVGRRGVLGIESPDPVLSLIGALGLAASAGTALVVDLAESWR
                     VGTSRTIADLLVDGPSLDELSPARSGVAVISAGPIDPAQALPIIERLAMNWPALVVRC
                     SAENWPGPKVPLRPLLPGMLAPTDVIPAVWQAMNGGVRPPGPGPVLPRLGSRTAHNLM
                     LGRLPTRGRWLRVWAKVWAMPWA"
     gene            1678..2727
                     /locus_tag="AC161X_02135"
     CDS             1678..2727
                     /locus_tag="AC161X_02135"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR4988379.1"
                     /translation="MIDQVAGQIASGSVPMRRGDVVVEAERRLRLAAPDRAAQLTEAV
                     VDRVLGLGPLEELLRDPDVTDVLVNGPEEVWVDRAGHLIRVDARFDSDDSLLAAVERV
                     IAPLGLRIDRSSPMVDARLADGSRLHAVAPPASVGHPVVAIRRFTQVVPTFEALVSLG
                     TATPSQAEVLEEAVRSRETIIVSGGTGAGKTTLLNLLGSLIPPTERVVTIEDAAELQL
                     PGHVIRLEAHPANAEGAGEITIRSLLKSALRLRPDRIIVGEVRGPEALDLVWALNTGH
                     RGSLSTVHANSPQEALWRLETLALSAGDTSEASVRRQLHAAVDYLVQIERVGAERQIQ
                     TVARVGLDGEIEDCS"
     gene            2724..3368
                     /locus_tag="AC161X_02140"
     CDS             2724..3368
                     /locus_tag="AC161X_02140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988380.1"
                     /translation="MIPALLIGWGVIHGLDRQRTLLLLLVVIAPVPFAGLLGVHAWRA
                     RPRVSTRAATFCEAVAGELRSGSSLRFALERAAASVDAPILERMSRDGAPFVEIGRRA
                     EEEFAEIGLELGAVIERLSRLGSPAAALFDEMGVLALAQVEVAHEIATATAPARATAV
                     VLLLVPLAAIGSATLNNRLGGYLESSPQRISALIGLSLVIGGLLVAGAILRRSK"
     gene            3365..3901
                     /locus_tag="AC161X_02145"
     CDS             3365..3901
                     /locus_tag="AC161X_02145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988381.1"
                     /translation="MIPIVLGSAGLVVGGPGLALALATAAKMHSMRRRRPGSPPIRPV
                     LLVLLVELRAGRSTLAALQQAALVFPTHHQLNLAARVATVKGLTSTVDECRGEVRRLV
                     AQLARAQKSGSAVADAVRAMLDADIAAERSRRLERARSLPIRLIIPITLFVLPGLVLL
                     LYAPSLLRLFSEISGPFS"
     gene            3953..4147
                     /locus_tag="AC161X_02150"
     CDS             3953..4147
                     /locus_tag="AC161X_02150"
                     /inference="COORDINATES: protein motif:HMM:NF025395.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4244 domain-containing protein"
                     /protein_id="HIR4988382.1"
                     /translation="MARIWTWVLARGDERGQGTVEYFLVILAATALAIVALKWLQSGS
                     GGGLLGGLFGKVIEWVVGRF"
     gene            4126..4506
                     /locus_tag="AC161X_02155"
     CDS             4126..4506
                     /locus_tag="AC161X_02155"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR4988383.1"
                     /translation="MGRRQVLTERGQATVEFFLVLPLVVLVAVASVQVMRIAEARLEL
                     QAAARDGARVAATTPDPSRAVASVMAALSPEDRDRARISVERPDRVGVPARVTVRLRH
                     LLGAPFPADMGVDLTVTATMRTER"
     gene            4524..4889
                     /locus_tag="AC161X_02160"
     CDS             4524..4889
                     /locus_tag="AC161X_02160"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rv3654c family TadE-like protein"
                     /protein_id="HIR4988384.1"
                     /translation="MTVLVIGWVAVIAVVGVAATSVGSLLAAREQAHTAAEAAALASA
                     VATYPPAGTGSPMALAGEYARRNGARLAACRCPIDLSIRPRTVTVTTALPVEVPIFGQ
                     VTVRASARSEFDPGLWLGG"
     gene            4906..5400
                     /gene="ybaK"
                     /locus_tag="AC161X_02165"
     CDS             4906..5400
                     /gene="ybaK"
                     /locus_tag="AC161X_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019630700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HIR4988385.1"
                     /translation="MATRTTRATDTLDRAGVTYTIHEYTVTEKVGEGYGEAVAAAIGL
                     PAGRVFKTLMAEVAGSPVVAVVPVTTRLSTRMLARATGNKRADMMSPADAERATGYVT
                     GGISPFGQKRALDMYLDSSALSHPTIAVSGGVRGLQLEVEPGDLMRVTGATVAVLVED
                     PADR"
     gene            5410..7140
                     /gene="polX"
                     /locus_tag="AC161X_02170"
     CDS             5410..7140
                     /gene="polX"
                     /locus_tag="AC161X_02170"
                     /inference="COORDINATES: protein motif:HMM:NF006375.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase/3'-5' exonuclease PolX"
                     /protein_id="HIR4988386.1"
                     /translation="MASRLDHLRMLAELAKLTALDEGSPQAFRVRAYENAIHGLQGHT
                     GEISGMDKEQLTSISGIGPSTADKILEFESTGRVAKLDTLREKYPPAFVELSKIPGLG
                     PKTLKLLRSELGVEDIDGLRAAIAEERLRDLPGMGKISEEKIGRSIERLGLHGKDRRT
                     ALVEALPLARALAGRLGALPSVSAAVPCGSIRRFSETVGDIDIVVITDDPASVAAFVL
                     EMPEASEFLGAGETKTSFLTREGMQVDVRLVSEDQMGSALLYFTGSKAHNISLRQRAI
                     ERGWLLSEYGLFEEERLVASTTEEEIYRSLGMQFVPPPLREGTGEVEAAAGDGLPALI
                     ARDQIQGDLHYHSTRSGDGRSTVEEMIDAAVANGYRYIAFTDHGEDLAINGSSREEML
                     AHRDRMRALQEEHPELRILFGCELNIGPKGDLDYDPDFRLQFDYCVASIHSHFDLSQS
                     EQTARILTALADPAVNAIGHLSGRYVGRRPGVELDIDAVLEGLAITGVALEINGALDR
                     LDASAEVSRLAMARDVDFVIDTDSHHVGDLVRMGYGVEYARRGWVTTDRVVNARPVDD
                     FLTWVGRRRG"
     gene            7284..>7417
                     /locus_tag="AC161X_02175"
     CDS             7284..>7417
                     /locus_tag="AC161X_02175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014988047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HIR4988387.1"
                     /translation="MADTFVLDTCVLLADPQSPLRFDEHDVVLPLVVIEELDRKKTRM
                     "
BASE COUNT         1190 a         2296 c         2522 g         1409 t
ORIGIN      
        1 gcccgggcct tctacgagcg actgggatgg gtcaagggaa agccgattcg cctggaaacg
       61 atcgggggga ccgagatcac cgaagttcgc tacgagacgg ctctagaact gccctgaccg
      121 cgtcgaggtc ggctggatag gtcactttga tgttcccggt ctcgccgggc acggccacga
      181 tcgtcaactc gctgaattcc ttcaccacgg ctgcggtgtc gtgcccttcg aatccggctg
      241 ccgccgctgc ccgataggca ctctgcagct cggggccaag gaacacctga ggcgtctggg
      301 cgatggcagt gtcagagacg ggccggagat ctgaacgtcg aaccagggga ttcgatgtcg
      361 ccaacaccgg caccgctcca ccggtttcgg ccgcgacctc gaacaaccgg ttcaccagac
      421 tctttgaggc caatggcctc gccccatcgt ggatcccgat gatgtcgatc gagtcaggaa
      481 ccgctcccaa acctgccagc tcgcttcggg tccgggtgac gccgccccgg accaggatca
      541 aattcggtgc caccttgctc atctcggtcg catgctcctc acgacagacg aggacgcaga
      601 cgtcgacgag cggcgccacc gcggcaacag tgtgtgccag cagtggctgt cccgcgattt
      661 gcgccatcag cttgtcgcca ccgaaacggg aactagtccc cccggccacg atgatcatcg
      721 cccgtcccga catcggcgtc gagggtactt tggtcaggtg atcgatatca gggcgcttcg
      781 gaagctgaca gaaggcggca aggaccacct cgacatcgac cacgccgggg ccctggtcga
      841 ccggggcgaa ttgaatgtcg agggccccgc ggtcctattc gtggtcccgg ttacggatgc
      901 ggtcaaggtg gtcgtcgatg acctggttct cgactcgttg gaccgagacg atctgtggtc
      961 gatccaggga tttcgccttg agcacgaagt gcttcttgtg ctccaggacg aggtgagcga
     1021 tccgcaatca ttggtcgaag cggtcgctgc cgccggcttc gtgtggcagg tgatccaccc
     1081 cacgacaggg gtcttgtgac gccctgatcg ggcctatagg ttcgggtgca tggagggggt
     1141 ggggagacgg ggcgtcctcg gcatcgagtc gcccgatccg gtgctttcgc tgattggcgc
     1201 actcgggctt gcggcttcgg caggcaccgc actcgtggtc gacctggcgg agagttggag
     1261 ggtgggaacc agccgcacca tcgccgatct cctcgtggac gggccctcgc tcgatgagtt
     1321 gagccccgcc cgatccggag tggcggtgat ctccgcaggg ccgatcgacc cggcacaagc
     1381 gctgccaatc atcgaaaggt tggcaatgaa ctggcctgct ctcgtggtgc gatgctccgc
     1441 cgagaactgg cctggcccca aggttccact ccgacctctc ctgcccggaa tgctggcgcc
     1501 gaccgatgtc attcctgccg tgtggcaggc gatgaacggc ggggtccgcc ccccagggcc
     1561 gggcccggtc ctgcccaggc tggggtcgag gactgcccat aacctgatgt tgggtcggtt
     1621 gccgacccgg ggtcgctggc tcagggtctg ggccaaggtc tgggcgatgc cgtgggcgtg
     1681 atcgatcaag tggccggcca gatcgcctcc ggctcggtcc cgatgcgcag gggtgatgtg
     1741 gtagtcgagg cggagcgaag gctgcggctt gccgcccccg accgcgccgc ccaactgaca
     1801 gaagctgtcg tcgatcgggt gctcggtctc ggcccattgg aggaactgct ccgcgacccc
     1861 gacgtcaccg acgtattggt gaacggcccc gaagaggtgt gggtcgaccg ggcgggccac
     1921 ctcatccggg tcgacgctcg tttcgacagc gatgactcat tgcttgcggc agtggagcgt
     1981 gtcatcgccc ctctggggct gagaatcgat cggtcctctc ccatggtcga tgctcggctt
     2041 gccgacggaa gccggttgca cgcggtggcg ccaccagcct cggtgggtca cccggtcgtc
     2101 gccatcagac gattcactca ggtggtgccg acatttgagg cacttgtttc gttgggaacc
     2161 gcaactccgt cacaggccga ggtgctcgag gaagcggtcc gttccaggga gaccatcatc
     2221 gtttccggtg gcaccggtgc cggaaagacg accctcctca atctcctcgg ctccctgatt
     2281 ccgccgaccg agcgggtggt gaccatcgag gatgctgccg aattgcaact gccgggccat
     2341 gtgatccggc tcgaggctca tccggccaat gctgaggggg caggcgagat caccattcgc
     2401 tccctcttga aatcggccct gcggctgcgt cccgatcgca tcatcgttgg cgaggttcgc
     2461 ggccccgagg ccttggactt ggtgtgggcc ctcaacaccg gacatcgtgg atcgctctcg
     2521 actgttcacg ccaattcgcc gcaggaggcc ctgtggcgcc tcgagaccct ggctctcagc
     2581 gccggcgaca cctctgaggc gtcggtgcgt cgccaactac atgctgcggt ggactatctg
     2641 gtccagatcg agcgcgttgg cgccgagcga cagatccaga cagtggcgag ggtcggcctc
     2701 gatggcgaga tcgaggactg ctcgtgatcc cggcgctcct catcgggtgg ggcgtgatcc
     2761 acggcctcga tcggcaacgc acgctgttgc tgctgttggt tgtgatcgcg ccagtgccct
     2821 ttgccggcct ccttggcgtt catgcctggc gggcgcgccc ccgggtctcg acacgagcgg
     2881 cgaccttctg tgaggcggta gccggcgagc tgagatcggg gtcttcgctg agattcgcct
     2941 tggagcgggc cgccgcatcg gtcgacgccc cgattctcga aagaatgagt cgcgacgggg
     3001 ctccgttcgt cgagatcggc cgtcgtgccg aggaggaatt cgccgagatc ggcctcgaac
     3061 tgggagcagt gatcgaacgt ctgtctcgcc tcgggtcgcc agccgcggcg cttttcgacg
     3121 agatgggggt cctggcgctg gcgcaggtcg aagtggctca tgagattgcc accgcgacag
     3181 ccccggcccg ggccacggct gtcgtcctcc tgctggtccc tctggccgcc atcggctcgg
     3241 ccaccctgaa caatcgcctc gggggatacc tcgagagctc gccacagcgc atttctgccc
     3301 tgatcggcct gtccttggtg atcggtggcc tcttggtggc gggagcgatc ctgaggcggt
     3361 ctaaatgatt cccatcgtgc tcggatcggc tggcctggtt gtgggcgggc cgggtctcgc
     3421 gctggcgctg gcgaccgcgg ccaagatgca ttcaatgcgg aggcggaggc cgggctcgcc
     3481 tccgattcgg cctgttctgt tggtattgct ggtggagttg cgagcgggaa gatccacgtt
     3541 ggcggcgttg cagcaggcag ccctggtttt cccgactcac caccaactca atcttgcggc
     3601 gcgagtggcg acggtgaagg gtctcacctc aacggtcgat gagtgtcgag gggaggtccg
     3661 caggttggtc gcccagttgg cgcgtgccca gaaatccggc tcggcggtgg ccgatgcggt
     3721 gcgcgccatg ctcgacgccg acatcgccgc cgagagatcc cggcgattgg aacgggcgag
     3781 gtcgcttccg attcggttga tcatcccgat caccctcttc gtgctccccg gactggtctt
     3841 gcttctctac gccccctcac ttcttcggct cttctccgag atctcggggc cgttctcttg
     3901 aaatccgcac ttctccccgt accggtcggt caccggattg gaaggagagc cgatggctcg
     3961 tatttggaca tgggtgctcg ccaggggaga cgagagaggg caggggacag tcgagtactt
     4021 cctggtgatc ctggccgcga ctgccctggc gattgtggct ttgaagtggc ttcaatcggg
     4081 tagcggcggc ggtctgctcg gtggtctttt cggcaaagtg atcgaatggg tcgtcggcag
     4141 gttctgaccg agagaggtca ggcgaccgtg gaattctttc tggtcttgcc tctggtggtg
     4201 ctggttgcgg tggcttcggt ccaggtgatg cggatcgccg aggctcggct cgaactgcag
     4261 gccgcggcga gggacggggc cagggttgcg gcgaccacgc ccgatccgtc ccgcgctgtc
     4321 gcctccgtca tggcagcgct ttccccggag gacagggatc gagcccggat ctcggtggag
     4381 cggccagacc gggtgggggt tcccgctcgg gtgacggtgc ggctccgaca cctgctggga
     4441 gcgccgttcc cggcggacat gggggtggat ctaacggtga cggcgacgat gcgcaccgag
     4501 aggtgatggc cgatcgaggg gggatgacgg ttctcgtcat cgggtgggtg gcggttatcg
     4561 ccgttgtcgg tgtggccgcc acctcggtcg gttccctcct cgcagcgcga gagcaggcgc
     4621 acaccgcggc ggaggccgct gcgctcgctt cggcggttgc gacatatccg cctgcaggga
     4681 ccgggtctcc gatggctctg gctggggagt atgcgcgacg aaacggggcg agattggcgg
     4741 cctgccgctg ccctatcgac ctctcgattc gcccgaggac cgtgaccgtg accacggccc
     4801 tgccggtcga ggttccaata ttcggccagg tcacggtgcg ggcatcggcg cgctcggagt
     4861 tcgatccggg gttgtggctt ggcggatagt gggacaatgt cggccatggc gacgaggaca
     4921 acccgggcaa ccgacactct ggatcgtgcc ggggtgacct acaccattca cgagtacacg
     4981 gtgaccgaga aggtcggtga gggttacggg gaggcggtgg ccgccgcgat cggccttccc
     5041 gccggccggg tgttcaagac actgatggct gaggtcgccg ggtcgccggt ggttgcggtg
     5101 gtcccggtta ccacccgact ctcgactcgc atgcttgctc gggcaacagg caacaagcgg
     5161 gccgacatga tgtcaccggc cgatgcggag cgggcgacgg gatatgtgac gggcggaatc
     5221 agtcccttcg gccagaagcg ggctctcgac atgtacctcg actcctcagc gctcagccac
     5281 cccacgattg ctgtgtcagg cggagtgcgc gggcttcagc tcgaggtcga acccggtgat
     5341 ctgatgcggg tcaccggcgc caccgtggct gtcctcgttg aggatcctgc agaccgatag
     5401 tctcgggata tggccagtcg tctcgaccat ctccggatgc ttgccgaact ggccaagctc
     5461 acagccctgg acgagggaag cccgcaagcg ttccgggtcc gggcctacga aaacgccatc
     5521 catggccttc aaggccatac cggtgagatc tcagggatgg acaaggagca actcacctcg
     5581 atcagcggga tcgggccgtc cactgccgac aagatcctcg agttcgagag caccggccgg
     5641 gtcgccaagt tggacaccct cagggagaag tacccgcccg cgttcgtcga gctgagcaag
     5701 atcccgggtc tcggccccaa gacactcaag ctgcttcgca gcgagctcgg ggtagaggac
     5761 atcgatggcc tccgcgccgc tattgcagag gaacgactgc gcgatctccc cggcatgggc
     5821 aagatctcag aggagaagat cggtcggtcg atcgaacgcc tcggcctcca cggcaaggat
     5881 cggcgcactg cattggtgga ggcgctgccc ctggcacggg ctcttgccgg gaggttggga
     5941 gctctgccat cggtatcggc tgccgtgccc tgcggctcga tccggcgctt ctccgagact
     6001 gttggtgaca tcgacatcgt ggtcatcacc gacgacccgg catcggtggc tgcgttcgtt
     6061 ctcgagatgc cggaggcatc cgagttcctg ggagccggcg agaccaagac ttcgttcctc
     6121 acccgcgaag ggatgcaggt agatgtgcgg ctggtgagcg aggatcagat ggggtcggcc
     6181 ctgctctatt tcaccgggtc caaggcccac aacatctccc ttcgccaacg agctatcgaa
     6241 cgggggtggc tgcttagtga gtacgggcta ttcgaggagg agcggctggt cgcttccacc
     6301 accgaggagg agatatacag gtcgctgggt atgcagttcg tgcccccgcc attgagggaa
     6361 gggaccggtg aggtcgaggc ggccgcgggg gacggtctgc cggctctgat cgcccgagat
     6421 cagattcagg gtgacctcca ctatcactcg acccgctccg gcgatgggcg ctcaacggtg
     6481 gaggagatga tcgatgccgc ggtggccaac gggtatcgat acatcgcctt caccgatcac
     6541 ggcgaggacc tggccatcaa tgggtccagc cgcgaggaga tgttggcgca ccgagatcgg
     6601 atgcgagccc tgcaggagga gcaccccgag ctgcgaatcc tttttggctg tgagctgaac
     6661 atcgggccca aaggcgactt ggattacgac ccggacttcc gtctccaatt cgactactgc
     6721 gtcgcctcga ttcattccca tttcgacctg tctcagtcgg agcagaccgc tcgcatcctt
     6781 accgcgctcg ccgatccggc agtgaacgcc attggccatc tctccggccg gtatgtgggt
     6841 cgacgtccgg gggtggagct cgacatcgac gcggtgctcg agggacttgc catcaccggt
     6901 gtggcgctcg agatcaacgg cgcactcgac cgactcgatg cctcggccga agtgagcagg
     6961 ctggccatgg cccgcgacgt ggactttgtt atcgacaccg actcgcatca cgttggtgac
     7021 ctggtgcgga tgggctacgg ggtcgagtac gcccgacgag ggtgggtcac cacggatcgg
     7081 gtggtcaacg ctcgcccggt cgacgacttt ctcacctggg tcggccgtcg tagggggtga
     7141 ccccttcgta attgggacgg gtaggtgtca tatgcggact tctgggtccc aattatgtga
     7201 gccggtccaa tcgatccttg taattgcgcg cctcggacgg aaagctatgc ctcctggcga
     7261 cgaccctgaa ggagacctaa gaggtggccg acacctttgt cctcgacacc tgtgtcctgc
     7321 tcgccgaccc tcaatccccg ctccgcttcg atgagcacga cgtggtcctg cctctagtcg
     7381 tcatcgagga gttggatcgg aagaagaccc ggatgga
//
LOCUS       DBNHXU010000083         4324 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_819289, whole genome shotgun sequence.
ACCESSION   DBNHXU010000083 DBNHXU010000000
VERSION     DBNHXU010000083.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4324)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4324
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_819289"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /locus_tag="AC161X_02180"
     CDS             complement(<1..500)
                     /locus_tag="AC161X_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012855273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HIR4988388.1"
                     /translation="MTTRRSGLGRGLDALIPSTPAVEPSGFRMVPVDSIVANPDQPRT
                     HFDDDTLEELAASMREVGVLQPIVVTDTGNGLVLIAGERRWRAAKRAGLATIPAVVRE
                     ATGSSTLVEALVENVQRQDLSPLEEANAYHHLLEDYGMTQEQVADRVGKSRPAISNTL
                     RLLQLPL"
     gene            complement(503..1294)
                     /locus_tag="AC161X_02185"
     CDS             complement(503..1294)
                     /locus_tag="AC161X_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018384195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HIR4988389.1"
                     /translation="MTDHGPLVVALANQKGGVGKSTTAINLAAALAFQGQRTLLIDLD
                     PQGNATSGLGIDRASIDVSIYDVMLKESELVDAIEPTSVRDLFVVPATIDLAGAEIEL
                     VSLFSRETRLRNAIQTSGDGHDVILIDCPPSLGLLTVNALTAADEVMIPIQCEYYALE
                     GLSQLLRNVQLVTTNLNPSLKVGGVVLTMYDGRTKLSKDVAQQVREHFGEITYRSVIP
                     RSVRLSEAPSYGEPIEAFDPMSRGAIAYRHLGKEFRERHGLKEKK"
     gene            complement(1291..1893)
                     /locus_tag="AC161X_02190"
     CDS             complement(1291..1893)
                     /locus_tag="AC161X_02190"
                     /EC_number="2.1.1.170"
                     /inference="COORDINATES: protein motif:HMM:NF014575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070043 - rRNA
                     (guanine-N7-)-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HIR4988390.1"
                     /translation="MKHSETWARCASWAGVELDSSALSRLEVYEQWLATEAIDAGGLG
                     PGERDRVHDRHIGDSLLFAGGLDNKPESVLDVGSGVGLPGIPLAIAMASIPFTLLDRS
                     VRRTDMLRRITRILDLENVTVEQGEIKGAPRQEAAIVSRATFPPDQARQVFLPLLRPE
                     GVAVLGGSWTRPPTDPGWTVIEIPPTVLDHRIWLLMMRQT"
     gene            complement(1980..2882)
                     /gene="jag"
                     /locus_tag="AC161X_02195"
     CDS             complement(1980..2882)
                     /gene="jag"
                     /locus_tag="AC161X_02195"
                     /inference="COORDINATES: protein motif:HMM:NF041568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding cell elongation regulator Jag/EloR"
                     /protein_id="HIR4988391.1"
                     /translation="MDYLEVRARTVDLAVEAAMTELGVTDREQLAVEVLQEPEKGFLG
                     FGGMDAVVRVTQRKTRRRRNERRRNPKEQRTDNRPRQEASKSGKNGDSRQKSNANSKG
                     RDMNRQDKNDQPRIDDRPVMTIEDQARIAGEFLEGLVAAYGLEGTVSTAVVEDVIVAD
                     VNGDQTEALVGVRGSVRSAIHELTRTVMQRYAQDTARLRLDIAGYAERRRQALTIYAE
                     QLIEQLMAEGGEIMLEPMSPADRKVIHDAAAAHEGVQSYSEGETPRRYVVLTRVDGDL
                     DAENEIDNDDEDAVDDTAYLSEEE"
     gene            complement(2884..3786)
                     /locus_tag="AC161X_02200"
     CDS             complement(2884..3786)
                     /locus_tag="AC161X_02200"
                     /inference="COORDINATES: protein motif:HMM:TIGR03592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0032977 - membrane insertase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YidC/Oxa1 family membrane protein insertase"
                     /protein_id="HIR4988392.1"
                     /translation="MGRLFSLLEQALGTVLAFFYDLLVPPFSEGNGLGLSIILLTIFI
                     NILVFPLTLKQTRATRAFQAIQPEIKRLQKEYKDDPQEMQKRLMALQKSAGATPGGCL
                     LPLLVQMPIWFALFRLLRTPLIFVEGSTTVLVEGAAHPVNPLSALGIALMDGSEKLFL
                     GMNLSVSPSQAFKVSLAVAVPYLILMAIMVASQYVQQWHSTYGQVRPTDQKGAGAQQA
                     LTKIMPLFIGFISWNFPAGLVLYWTTSNLFRLGQQALIFKLDGRPTPGATPDESDQAI
                     ESDNGDPKKPQTGAVDKRRRRRRR"
     gene            complement(3786..3992)
                     /gene="yidD"
                     /locus_tag="AC161X_02205"
     CDS             complement(3786..3992)
                     /gene="yidD"
                     /locus_tag="AC161X_02205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015166977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HIR4988393.1"
                     /translation="MIMIYQKLVAPNLRANCRYQPTCSHYTAEAITRFGVIRGSWLGM
                     KRIGRCHPLRPGGYDPVPETWRAQ"
     gene            complement(4024..>4324)
                     /gene="rnpA"
                     /locus_tag="AC161X_02210"
     CDS             complement(4024..>4324)
                     /gene="rnpA"
                     /locus_tag="AC161X_02210"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: protein motif:HMM:NF013022.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004526 - ribonuclease P activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HIR4988394.1"
                     /translation="PRDFRRVLNEGTRRRSGAVVMVQCPGHAGPPRVGLVVSKSAGNA
                     VTRNRIKRRLRQIVRTLPLKPGIDYVIIGASQVAEVSHPQLVGWMERALEGSGDA"
BASE COUNT          833 a         1337 c         1274 g          880 t
ORIGIN      
        1 agtggaagct ggagtagccg caaggtgttg gagatcgcag gtcgtgactt cccgacccga
       61 tcggcgacct gctcctgggt cattccataa tcttccagga ggtggtggta ggcgttcgcc
      121 tcctccagcg ggctcagatc ctggcgctgc acattctcaa ccagagcctc gaccagcgtc
      181 gacgaaccgg tggcctcgcg aacaacggcc gggatcgtgg caagcccggc tcgtttcgcg
      241 gcccgccatc tccgctctcc ggcgatcagc accaacccgt tgccggtgtc ggtgaccaca
      301 atcggctgga ggacacccac ctcccgcatc gaggcggcca actcctcgag ggtgtcgtcg
      361 tcgaagtggg tccgtggctg atcgggattg gcgacgatgc tgtccaccgg gaccatcctg
      421 aaaccactgg gttcgaccgc gggcgtggat gggatcaggg catccagacc acgacccaga
      481 ccggagcgtc ttgtcgtcat cgctacttct tctccttcag cccatgccgt tcccggaatt
      541 ccttgcccag gtggcggtag gcgattgccc ctcgactcat cgggtcaaag gcctcgatcg
      601 gctctccata tgacggtgcc tcggagagcc gcaccgagcg ggggatgaca ctgcgatagg
      661 tgatctcccc gaaatgctcg cgcacctgtt gtgccacgtc cttggacagt ttggttcggc
      721 cgtcgtacat ggtgaggaca actcccccaa ccttcaagct ggggttgagg tttgtggtca
      781 ccaattgcac gtttctgagg agctggctga gcccttcgag tgcgtagtac tcacattgga
      841 tcggaatcat cacctcatcg gccgcggtga gagcgttgac cgtgagcaaa cccaacgacg
      901 ggggacagtc gatgagaatc acgtcatgtc cgtctcccga ggtctggatc gcgtttcgga
      961 gtcttgtttc acgtgaaaac aacgaaacca gctcgatttc ggcgccagcg agatcaatag
     1021 tggccggtac cacgaacaaa tcccgcaccg aggtgggttc gatggcgtcg accaactccg
     1081 actccttgag catcacgtca tagatggaga catcgatcga ggcccgatcg attccaagac
     1141 cgctggtggc attgccctgg ggatcgagat cgatgagcaa tgtacgttgc ccctggaatg
     1201 ccaacgccgc agccagattg atcgccgtcg tcgatttgcc gacaccaccc ttctggttgg
     1261 cgagggccac gaccaatggt ccatgatctg tcacgtttga cgcatcataa gaagccagat
     1321 cctgtggtca aggaccgtgg gtgggatctc gatcacggtc caccccggat cggtgggtgg
     1381 tcgggtccag gatccgccga gaacagccac cccttccggg cgaagcagcg ggaggaacac
     1441 ctgtcgggct tgatccgggg gaaaggtggc ccgggagacg atcgccgctt cttgtcgagg
     1501 ggcacctttg atttcccctt gttccaccgt gacattttcc aggtccagga tccgggtgat
     1561 ccgtcgcaac atgtcggtgc gacgaacgga gcgatcaagc aaggtgaatg ggatgctcgc
     1621 catggcgatg gcaaggggaa tcccgggtag cccgacaccg gagccaacat cgagcacgct
     1681 ctcaggtttg ttatcgagcc cgccggcgaa aagcagcgag tccccgatgt gccggtcatg
     1741 tacccgatct cgctccccgg gccccagacc accggcatcg atcgcctcgg tggccagcca
     1801 ctgctcgtag acctcgagtc gcgatagagc actcgaatcg agctccacgc cagcccatga
     1861 cgcacagcgg gcccaggtct cagagtgttt cacgtgaaac cggactctat cggtcgtagg
     1921 aagccggcca tcggaaaagg gagcccgtag gcccccttcc catccaaatc agttgaggat
     1981 cattcctctt cggacagata agcggtgtcg tccaccgcat cctcatcatc gttgtcgatc
     2041 tcgttctcgg catcgaggtc gccatcgacc cgggtcaaaa ccacgtaacg acggggggtt
     2101 tcgccttctg aataggactg caccccttca tgggcggcgg ctgcgtcatg gatcactttg
     2161 cgatcggcag gactcattgg ctccagcatg atctccccac cttcggccat cagctgttct
     2221 atcagttgct cggcgtagat ggtcagtgcc tgacgacgtc tttccgcgta tcccgcgatg
     2281 tcgagtcgaa gccgcgccgt atcttgggca taacgctgca tgacggttcg ggtcaactca
     2341 tgaattgcgg agcgcaccga gccccggacc ccgaccaggg cctcagtctg atcaccgttg
     2401 acgtctgcga cgatcacatc ctcgacgacg gcagttgaaa ccgtgccctc gagcccgtag
     2461 gcggcgacca aaccctcgag gaattcaccg gcgattcgag cctggtcctc gatcgtcatc
     2521 accggacgat catcaatcct gggttgatca tttttgtcct gacgattcat atcgcgaccc
     2581 ttgctgttgg cattgctctt ctgtcggctg tccccattct tacccgattt cgacgcttct
     2641 tgtcggggtc gattgtccgt gcgttgctct ttggggttgc gtcgccgctc attgcgacgc
     2701 cgtctcgttt tgcgctgggt gactcgcacc acagcatcca tccctccaaa gccaagaaaa
     2761 cccttctccg gctcctggag aacttcgacg gcaagctgct cacggtcggt gactcccaat
     2821 tcggtcatcg ccgcctcaac cgccaaatcc acggtacgcg cccgtacctc tagataatcc
     2881 acgtcatctc ctcctgcgcc tgcggcgctt gtcaactgcc cccgtctgcg gtttcttggg
     2941 atcgccgttg tcggattcga tcgcctggtc tgattcgtcc ggtgtggcac cgggggtggg
     3001 acgcccatcg agtttgaaga tcaacgcctg ctggccaagc cggaacaggt tggaggtggt
     3061 ccagtagagg accaggccgg ccgggaagtt ccatgagata aacccaatga ataagggcat
     3121 tatcttggtc agagcctgct gggccccggc gcccttttgg tcggtgggcc gcacctggcc
     3181 gtaggtcgaa tgccattgct gcacgtattg gcttgccacc atgatcgcca tcaggatcag
     3241 ataggggacc gcgaccgcca ggctgacctt gaaggcctgg ctgggggaca cgctcaagtt
     3301 catcccgagg aacagcttct cggacccatc catcagagcg atccccaaag cggacagtgg
     3361 attcaccgga tgggccgctc cttccaccag cacggtggtg gatccctcca cgaagatcag
     3421 gggagtcctc agcagtcgga aaagggcgaa ccaaatcggc atctggacca acagcgggag
     3481 caaacagccc ccaggggtgg cgccggccga tttttgcaac gccatgagac gcttctgcat
     3541 ctcctggggg tcgtccttgt actccttctg tagccgcttg atctccggct ggatggcttg
     3601 gaaggctcgg gtggccctgg tctgcttcag cgtcagcgga aaaaccagga tgttgatgaa
     3661 aatggtgagg aggatgatgg agagcccgag cccgttgccc tcgctgaatg gcggcaccag
     3721 gaggtcgtaa aagaaagcga gcactgtgcc caaggcctgc tcgagaaggc tgaacagacg
     3781 acccattatt gagctctcca ggtttcaggg accgggtcgt agccacccgg gcgcagtgga
     3841 tggcatctgc cgatccgctt cataccgagc caactgccac gaatcacacc aaatcgggtg
     3901 atggcctcgg cggtgtagtg agagcacgtc ggctggtatc ggcagttcgc cctcaggttg
     3961 ggagctacca atttctggta aatcatgatc atccccaccg ccaggaatcg agggcgcagc
     4021 caatcaggca tcgccggatc cttcgagggc gcgttccatc catcccacca actgggggtg
     4081 agagacctct gctacctggc tggcgccgat gatcacgtag tcaattcctg gtttgagcgg
     4141 cagcgtccgc acgatttggc gcagacgacg tttgatccgg ttccgggtca ccgcattccc
     4201 ggcactcttc gagaccacga gcccgactcg aggtggcccc gcgtgtcctg gacattgaac
     4261 catgaccacg gcaccagagc gacgtctggt tccctcattc aagacccttc gaaaatcgcg
     4321 cggg
//
LOCUS       DBNHXU010000084         2070 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_225376, whole genome shotgun sequence.
ACCESSION   DBNHXU010000084 DBNHXU010000000
VERSION     DBNHXU010000084.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_225376"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="AC161X_02215"
     CDS             complement(<1..664)
                     /locus_tag="AC161X_02215"
                     /inference="COORDINATES: protein motif:HMM:NF024221.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HIR4988395.1"
                     /translation="MANPVLERQFGTATADSALQTTPTPPADGRSTMSIGSVILSTIV
                     LLALVMAGAAYGWANAAAVQRWYWLLAVTMIALLIATVARPKLVVLTGVFYSLVQGAL
                     LGSLSKIYETWYDGIVFQALLGTLAVFVAMLFLYGTRIIKVTQRLRSVIIVATVGLAL
                     FYLFSWVLIAFGVDIPVVTGAGTGAAVFTLVVIVVAALNLLLDFQVIELGVRSGAPRE
                     YSW"
     gene            756..1724
                     /gene="nadA"
                     /locus_tag="AC161X_02220"
     CDS             756..1724
                     /gene="nadA"
                     /locus_tag="AC161X_02220"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR4988396.1"
                     /translation="MTPIAITRAADVLPEAEVVALQPLIDEIDWWKKERNAIVAAHNY
                     MTPDIYHLAADIKGDSLALAQIAVDTEADVIVLAGVHFMAETAKIINPQKMVLIPDLR
                     AGCSLADGITGADVRALREQHPGVPVVTYVNTSAEVKAESDITCTSSNAVAVVESLGV
                     DKVIFTPDQYLGRWVASQTDVEVVLWEGSCMVHERFTGQEIADYRRGYDGIKVIAHPE
                     CPPDVLAEADYVGSTHGMIQWVTTNQPARVMMITECSMADNVASETPNTEYIRPCNLC
                     PHMKRITLENIRDSLVTLTHQIEIDPAIAERARAAVSRMLEVGRQD"
     gene            1734..>2070
                     /locus_tag="AC161X_02225"
     CDS             1734..>2070
                     /locus_tag="AC161X_02225"
                     /inference="COORDINATES: protein motif:HMM:NF013086.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HIR4988397.1"
                     /translation="MTDITRLDAPLVVGAGVAGLTVALGLPRAYVMSAPEMGSTWWAQ
                     GGIAVALSEEDSPQQHAEDTVNVSGGLAVRNVVDVLTKGGPAAIENLIRLGAEFDRDD
                     DGGLSLGQEG"
BASE COUNT          417 a          667 c          643 g          343 t
ORIGIN      
        1 accaggagta ctcccttggc gctccactgc gcaccccgag ctcgatgacc tgaaaatcga
       61 ggagcagatt gagcgcggcg accacgatca cgaccagggt gaacaccgcg gctccggtcc
      121 ccgccccggt gaccaccggg atgtcgactc cgaaggcaat gagcacccaa ctgaagaggt
      181 agaagagggc caatcccacg gtggcgacga tgatcaccga gcgcaaacgc tgggtgacct
      241 tgatgattct ggtgccgtag aggaagagca tggccacgaa gactgcgagg gtgccgagca
      301 acgcctggaa gacgatcccg tcgtaccagg tctcgtagat cttggagaga gagccgagca
      361 gcgccccctg aaccaacgag tagaacaccc cggtgaggac aaccagtttc ggcctggcca
      421 ctgtggcgat caacagggcg atcatggtca ctgccaggag ccaataccag cgttggaccg
      481 cggcagcgtt ggcccatccg taggccgccc cggccatcac cagggcgaga agcacgatcg
      541 tcgagaggat caccgatccg atcgacatcg tggagcgccc gtcagctgga ggagtgggag
      601 tggtttgtag cgcggagtca gccgtggccg tgccgaactg cctttcgaga acagggtttg
      661 ccatgagccg agtgtatttg tcggtcggct gcagacggcg gtcttggagt cggcctgggt
      721 atctggcctt tggccggccg tacactcgtc acctgatgac accaatcgcc atcacccgcg
      781 ccgcggatgt cctccccgag gccgaggttg tcgcgctcca accgctgatc gacgagatcg
      841 actggtggaa gaaggagcgc aacgcgatcg tcgctgctca caactacatg acgccggaca
      901 tctatcacct ggcggcagat atcaagggcg actcactcgc actcgctcag atcgcggttg
      961 acacggaggc ggacgtcatc gtcctggccg gggtccactt catggccgag accgccaaga
     1021 tcatcaaccc ccaaaaaatg gtgctcatcc cggatctgcg cgccggctgc tctctcgccg
     1081 acgggatcac cggcgccgac gtccgcgcgc tgagagagca acaccctggc gtccccgtgg
     1141 tcacttacgt caacacctcg gccgaggtca aggccgagtc cgacatcacc tgcacctcga
     1201 gtaacgccgt tgccgtggtc gagtccctcg gtgtcgacaa ggtcatcttc actccggacc
     1261 agtatctggg gagatgggtg gcatcccaga ccgacgtgga agtggttctc tgggaggggt
     1321 cctgcatggt ccacgagcgc tttaccggtc aagagatcgc cgactaccgc cggggatatg
     1381 acgggatcaa agtgattgcc catcccgaat gcccgcccga tgttctggcc gaagccgact
     1441 acgtcggctc cacccacggg atgatccagt gggtcaccac caaccagccc gctcgggtca
     1501 tgatgatcac cgagtgctcg atggcggaca acgtcgcgag cgagacgccg aataccgagt
     1561 acatccgccc ctgcaacctc tgtccgcaca tgaagcggat caccctcgag aacatcaggg
     1621 attcgctggt caccctgacc caccagatcg agatcgaccc tgcaatcgcc gagcgagccc
     1681 gggccgccgt ctcccgtatg ctcgaagtcg gccgccagga ctagaactac ccaatgacgg
     1741 atatcacgcg cctcgacgcc cctcttgtcg ttggcgccgg cgtggccggg ctcaccgtgg
     1801 cgctcgggct tccccgcgcc tacgtcatgt cggcgccgga gatgggctcg acctggtggg
     1861 cccagggagg catcgcagta gcgctttcgg aggaggattc cccacagcaa cacgccgagg
     1921 acaccgtcaa cgtttccggt ggccttgcag tcaggaatgt cgtcgacgtc ctcaccaagg
     1981 gaggcccggc ggcgattgag aacctgatcc gtctcggtgc tgagttcgac cgggatgatg
     2041 acggcggcct ctcccttgga caggaaggag
//
LOCUS       DBNHXU010000085         2470 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1415928, whole genome shotgun sequence.
ACCESSION   DBNHXU010000085 DBNHXU010000000
VERSION     DBNHXU010000085.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2470)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2470)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2470
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1415928"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="paaC"
                     /locus_tag="AC161X_02230"
     CDS             complement(<1..659)
                     /gene="paaC"
                     /locus_tag="AC161X_02230"
                     /EC_number="1.14.13.149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011442518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaC"
                     /protein_id="HIR4988398.1"
                     /translation="MLLGHADDNLVLAQQLGGWISRAPELEEDIALGNIALDHLGVAR
                     ALYTLAGAVEGQGHDEDDLAMFRDEHDYRNLLLVEQPNGDFANTIVRQLFFDSYQVPL
                     WEDLASSEDLELAGIAARALKEARYHFRHSSGWVIRLGDGTEESASRTERAVDALWPF
                     TEEMFDGATAGYRPSWERTVVSVLAEARLGVPEGSHQQSGGRDGRHSEHLASLLAEMQ
                     W"
     gene            complement(701..964)
                     /gene="paaB"
                     /locus_tag="AC161X_02235"
     CDS             complement(701..964)
                     /gene="paaB"
                     /locus_tag="AC161X_02235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014202586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaB"
                     /protein_id="HIR4988399.1"
                     /translation="MWEVFVRARRGVSHVHIGSLHAPDSETALQNARDVYTRRGEGVS
                     VWVVPSSEIRSLEPDEAADYYEANEKVYRAATAYDIPDEVGKM"
     gene            complement(1042..1971)
                     /gene="paaA"
                     /locus_tag="AC161X_02240"
     CDS             complement(1042..1971)
                     /gene="paaA"
                     /locus_tag="AC161X_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006348010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097266 - phenylacetyl-CoA 1,2-epoxidase
                     activity [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1,2-phenylacetyl-CoA epoxidase subunit PaaA"
                     /protein_id="HIR4988400.1"
                     /translation="MTPEQRFQEIVDAEDKIEPTDWMPDAYRQTLIRQIAQHAHSEII
                     GMQPEGSWIGRAPSLHRKAILMAKVQDEAGHGLYLYSAAETLGVRRDELQELLHQGKH
                     KYSSIFNYPTLNWADIGTIGWLVDGAAITNQVMLTRCSYGPYARGMVRICKEESFHQR
                     QGYELLMRLCQGTPEQKALAQDALDRWWWPSLMMFGPHDTESTHTEQSMAWKIKRVSN
                     DELRQQFVDMTVPQADYLGLRIPDPELRWNEERGHHDFGEIDWDEFWAVVKGDGPLNR
                     ERIAHKAEAWREGEWVREAALAHAEKQAARGAA"
     gene            complement(2064..>2470)
                     /locus_tag="AC161X_02245"
     CDS             complement(2064..>2470)
                     /locus_tag="AC161X_02245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988401.1"
                     /translation="STVSPDQPVDSGGSDQPISEPGPVGSIPEPRPPIDGSVNGDVSI
                     TKADLLIMESYPIQVSLRVEGEKPTPCHEIFWTVEDDGETIQITMISQVNSDQSCAQV
                     ISPFAIAVPLGSWAEESREVVLNGENVGSFDS"
BASE COUNT          435 a          817 c          740 g          478 t
ORIGIN      
        1 atccattgca tctcggcgag aagcgaggcg aggtgctcgc tgtgacgacc gtcacggcca
       61 ccggattgct ggtgcgagcc ttcgggaacg ccgagccttg cttcggccag gaccgacacc
      121 acggtccttt cccacgacgg gcgatacccg gccgtggctc catcgaacat ctcttcggtg
      181 aatggccaca acgcgtcgac ggcccgctca gtgcgactcg ccgactcctc ggtcccgtcg
      241 ccgagccgga tcacccatcc cgaggagtgc ctgaagtgat agcgggcttc cttcagagcg
      301 cgagccgcaa tccccgccaa ctccaggtcc tccgacgacg ccaggtcctc ccaaagcggc
      361 acctggtaac tgtcgaagaa gagctgacgc acaatggtgt tggcgaagtc accatttggc
      421 tgctccacca gcagcaggtt ccgatagtca tgctcgtccc ggaacattgc gaggtcgtcc
      481 tcgtcgtgac cctgtccttc cacagctccc gccagcgtgt agagagccct ggcgacaccc
      541 aggtgatcca gtgcgatgtt tcccagagca atgtcctcct cgagctcggg cgcccgggag
      601 atccagcctc cgagctgctg ggcgaggacc aggttgtcgt cggcgtgtcc gagcagcatg
      661 gcgaaaccct gagacctgag acctgagacc tgagactggc tcacatcttc ccgacttcgt
      721 cggggatgtc atatgcggtc gccgcccgat acaccttctc attggcctcg tagtagtcag
      781 ccgcctcgtc aggctccaga gatcggatct cactgctcgg caccacccag acgctgaccc
      841 cttcccccct gcgggtgtag acgtcccggg cgttctgcag ggcagtctcc gagtcgggcg
      901 cgtgaaggct gccgatatgg acgtgcgata ccccgcgtcg ggcccggacg aacacctccc
      961 acatctgcca tgtgccaccg gcgacctcaa cgctggtctc gatctcgccg ctctcccctg
     1021 gggccaccgg gacttcgtag gtcacgccgc gccccttgct gcctgcttct cggcgtgagc
     1081 cagggctgcc tcccttaccc actccccttc gcgccatgcc tcggccttgt gggcgatgcg
     1141 ctcccggttc aacggcccat cacccttgac tacggcccag aactcatccc aatcgatctc
     1201 accgaaatca tggtggcccc gctcctcgtt ccagcgcagc tcgggatcag ggattctcaa
     1261 cccgaggtag tcagcctggg gaacggtcat gtcgacaaac tgctggcgaa gctcgtcgtt
     1321 ggacactctc ttgatcttcc aggccatcga ctgctcggtg tgggtggact ctgtgtcgtg
     1381 gggcccgaac atcatgagag atggccacca ccagcggtcg agcgcgtcct gagccagcgc
     1441 cttctgctcg ggggtgccct gacaaagcct catgaggagc tcgtaaccct ggcgctggtg
     1501 aaacgactcc tccttgcaga tccggaccat cccccgggcg taaggcccgt aggagcagcg
     1561 ggtgagcatc acctggttgg tgatggccgc gccatcgaca agccatccga tcgtcccgat
     1621 gtcagcccag ttcagggtcg gatagttgaa gatcgaggag tacttgtgct tgccctgatg
     1681 caatagctcc tggagctcgt ccctcctgac accgagggtc tccgcggccg agtagaggta
     1741 gagaccgtgc ccggcctcgt cctggacctt ggccatgagg atggccttgc gatggagact
     1801 cggcgccctg ccaatccagc ttccttcggg ctgcatgccg atgatctcgg aatgggcatg
     1861 ctgggcgatc tgtcggatca gggtctgcct gtaggcgtcg ggcatccagt cggtgggctc
     1921 gatcttgtcc tcggcgtcga cgatttcctg aaaacgctgt tcgggggtca ccgggcgaga
     1981 atactacaaa cgaacgttcg ttggtaacga gaggccattc agaaacctgc cggatcgggg
     2041 ccgggtcgag ccggtgtgcc tgactagctg tcgaaagagc ccacgttctc accgttgagg
     2101 acgacctccc gcgactcctc cgcccaacta ccgagaggca ccgcaatcgc gaagggggag
     2161 atcacctggg cacagctctg gtcgctgttg acctgggaga tcatcgtgat ctggatggtc
     2221 tcaccgtcgt cctcaacggt ccaaaagatc tcgtgacagg gagtcggctt ctcaccttcg
     2281 acccggagac tcacctggat cgggtagctc tccatgatca agagatcagc cttcgttatg
     2341 gagacatcgc cgttcacgct cccatcgatc ggtggtcgcg gctcgggaat ggagccaacc
     2401 ggaccgggct cggagatcgg ctgatcgctg ccgccggaat ccaccggttg atcgggagag
     2461 acagtgctcg
//
LOCUS       DBNHXU010000086        10003 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2010605, whole genome shotgun sequence.
ACCESSION   DBNHXU010000086 DBNHXU010000000
VERSION     DBNHXU010000086.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 10003)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10003)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10003
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2010605"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..213
                     /locus_tag="AC161X_02250"
     CDS             <1..213
                     /locus_tag="AC161X_02250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HIR4988402.1"
                     /translation="SWSSPAVVDGMLVTATCNGDVRGYSLEDPRSPVRQWTVDLGETC
                     LESTPAIWNGAIYIGSRDGYLRALAP"
     gene            210..728
                     /locus_tag="AC161X_02255"
     CDS             210..728
                     /locus_tag="AC161X_02255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988403.1"
                     /translation="MRRPIVLVLLAALTACGSAGGDATTTSPVNTTTTVGETTTTETP
                     VDCPAPPYELEFLPTGLGTGLLDPDEIDMDVWTSVGGSQTAFYGRQDGTVAIALVRGT
                     LPPVEWPAEKGEVLLDGTRGVVGPHPDGTWVAGWYEEPGERCDLYTMVFYPPVAPTDV
                     ESVIAGMNRVGG"
     gene            953..2332
                     /locus_tag="AC161X_02260"
     CDS             953..2332
                     /locus_tag="AC161X_02260"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I family
                     protein"
                     /protein_id="HIR4988404.1"
                     /translation="MNHPFDIPADLDTPTSVYLKLGPLHPRFLLESVEHGDRVGRYSF
                     IGFGDGLEVRSDGRSVDIGETRVETDGTVKGLMDALRKALAAAPRPGPLSGSVPFAGG
                     LVGAVGFDVAHRLAGAIPGPSATPEVLLYGPSSVIVFDHLSRRAALLHDGSEAERLAL
                     RQEVISLLAGGVPGIRNGGGSFSEPLPSMKRDDYLAAVEEAREHIAAGDVYQLVVSVR
                     FAGESDLDPFDVYRGLRLLNPSPYMYFCELGETRVIGSSPEALVRLRGTAAELRPIAG
                     TRPRSNDEEHDRRAEEDLLDDAKEAAEHVMLVDLARNDLGRVAVPGTIRVDPYRVIER
                     YSHVMHLVSGVTGILEPGADAFDLFAAAFPAGTVVGAPKVRALELLQELEAVPRGIYA
                     GTVGYFGHGGSMDQAIAIRTLVMSEGSYSYQAGAGIVADSVPATEWDEVLSKSGAMRG
                     ALESAAEGL"
     gene            2329..2913
                     /locus_tag="AC161X_02265"
     CDS             2329..2913
                     /locus_tag="AC161X_02265"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009671910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component II"
                     /protein_id="HIR4988405.1"
                     /translation="MSRVLLVDNYDSFTYNVVQALRILGAEVIVRTNDDIDVESAREL
                     EPTHLVISPGPGGPEDAGESMNVISGLLTEVPILGVCLGHQALAAALGGKVQRADELR
                     HGKASPVYHDSATIYAGLPNPFDAGRYHSLAVQEEALPSELVVTSFTSDGVIMGVRHR
                     EYPVEGVQFHPESVLTPHGDLLLRNFLDMTGTKS"
     gene            2910..3926
                     /gene="trpD"
                     /locus_tag="AC161X_02270"
     CDS             2910..3926
                     /gene="trpD"
                     /locus_tag="AC161X_02270"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR01245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HIR4988406.1"
                     /translation="MRELLDDLLAGTDLSTPAADELLVNLTSGNVEPAMAGAILAALR
                     AKGETADEVRGFASAMRRLSSDPGIDASGAVDVVGTGGDRSGSLNLSTGAALLTAASG
                     VPVVKHGNRSMTSQSGSADILEAIGLPIPLDADQARRCFETVGFTYLFAPHYHPAMAA
                     VGPIRKALGVRTVFNMLGPLTNPAGPRHLVVGAFDARAAELMAGALSGLEIDRAFVLH
                     GAAGWDEPSPIGEFLLYDVQPGSVVRSTRDPVDLGMQRCDPRELAGGDPAHNAAQLTA
                     VFSGESGPHRDSILLGAALALEVTGRATNPADAVEVARSALDDGRAMALLQGLQLFGA
                     GERA"
     gene            3923..4732
                     /locus_tag="AC161X_02275"
     CDS             3923..4732
                     /locus_tag="AC161X_02275"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF012444.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HIR4988407.1"
                     /translation="MSRFLQSMEQSSRSRLELARSRRSLGAITASALDAPSVRDVGTF
                     GEVFDLIAEIKPRSPAEGELAGSELVERAIAYQEGGARMLSVLTEPEAFGGSLALLEA
                     VAAVARVPVLRKDFLVDPYQVFESRAFGADCVLLIARLLSDQAMADLLDAVEETGMVA
                     LLEAFDATELSRVNSIAETSPGTLVGVNCRDLETLHTVPERHDELAPGLSLTIPSVAE
                     SGILGTDDARRVAGLGYRAALVGSALMRSDDPADLARTMLAAGQESAAVAS"
     gene            4729..5352
                     /locus_tag="AC161X_02280"
     CDS             4729..5352
                     /locus_tag="AC161X_02280"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: protein motif:HMM:NF012900.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HIR4988408.1"
                     /translation="MSLFIKLCGLKTDADVETAVEAGADAVGFVLTPSSRQIALFEAA
                     KLRRLLPESVLAVAVFNDPSPNLIEQALEEVAPDLVQVEARNGTGVPIQKFLPVVLNG
                     NGAPEAVMQALELTESGMVLVDTAEKGGTGRRSDWGRLARLKVRDRVIVAGGLNPGNV
                     GEAISTIGPLGVDVSTGIERRPGEKDPVLMRQFVRAARAAEVRGASR"
     gene            5349..6512
                     /gene="trpB"
                     /locus_tag="AC161X_02285"
     CDS             5349..6512
                     /gene="trpB"
                     /locus_tag="AC161X_02285"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005224573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HIR4988409.1"
                     /translation="MSTAGRFGSFGGSYVPEILVPALTRLEETATRLLADPGFLAMYE
                     TELSSWVGRPTALTRAARLGEAWGGEIWLKREDLAHTGAHKINNAIGQALLAREMGAG
                     RVIAETGAGQHGVATAAACARLGLDCTVYMGTVDIERQAPNVERMKLLGAKVSPVVSG
                     DATLRAAINEALRDWVTDPNSTYYLLGSAVGPHPYPWLVRELQSVIGREARQQMLDRA
                     GRLPDVAFACVGGGSNAIGLFHPFLDDPVQLIGVEAGGRGPGLGENAATIGRGSPGVL
                     HGSYSFILQDGDGQIQETHSVSAGLDYPGVGPEHAWLAETGRASYVEASDDEALSALD
                     ELGRLEGILPALESSHALAAVRRWLPATPGAVVLVGLSGRGDKDIDILRGLNR"
     gene            6509..7276
                     /gene="trpA"
                     /locus_tag="AC161X_02290"
     CDS             6509..7276
                     /gene="trpA"
                     /locus_tag="AC161X_02290"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HIR4988410.1"
                     /translation="MTTALTGAGLLEQRLRADAPAVVPYLTAGFPTIERFADALAAVA
                     AISEAVEIGIPFSDPMADGATIQESSRIALEAGATLELVISALESIGALPCPLVVMSY
                     LNPLMAFGFDRLLPRLSGVGVAGLVVPDLPLDESEDLRGVAAEHGVGLVQLVTPVTED
                     DRLRRLGEASEGFTYAVTMTGTTGGSAGVGSDVAGYLDRVRGVSRAPVLAGFGVRSSD
                     QVRSLVPHCDGVIVGSALVEVLGREEDPVAFLTGLRP"
     gene            7273..8313
                     /gene="aroF"
                     /locus_tag="AC161X_02295"
     CDS             7273..8313
                     /gene="aroF"
                     /locus_tag="AC161X_02295"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009709781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HIR4988411.1"
                     /translation="MIATLTADCTLAQKAAILRFIEGYGLRVHVGEAGSTTVVSVIGD
                     YEPGMAESIGALLGVDSVSTEAPLYPFVARAARNSPTTVRVGDVVVGGPEIVVIAGPC
                     SVESAAQIDRIATGVAKEGAVLLRGGAFKPRSSPYSFQGMGEDGLELLAAARESTGLG
                     VVSECVAPEDVPLLNRYVDMLQVGSRNMQNFRLLSAVGEQPKPVLLKRGLMSTIEELL
                     LAAEYIVGSGNPRVILCERGVRSFDTATRNILDISAVPVLKERTHLPVIIDPSHAAGR
                     RSLVPSLAAAGIAAGADGLIVEVHDRPEEALSDGPQSLTLEGFAETMGTVRRVAAALD
                     RTVSTLGVVRTT"
     gene            complement(8329..9252)
                     /locus_tag="AC161X_02300"
     CDS             complement(8329..9252)
                     /locus_tag="AC161X_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012228736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR4988412.1"
                     /translation="MEAVIILFAVMALVLILIAAAGFKVVRPYEKGLIEFLGRYQRTV
                     DSGLRWVLPFVKRLTKVDMRERVIDVPPQEVITKDNVVVTVDAVIYYEATDPVKLEYN
                     VGNFMLAVTKLAQTNLRNVVGDLDLDSALTSREVINAKLRQILDDATDKWGVRVVRVE
                     IQRIEPPTDVTDAMHRQMKAERDRRAAVTEAEGLKRAAILKAEGLKESQVLEADGQAE
                     AIKRVADAEKYQKLTVAEGEGQAIERVYAAIHNGNPSPDLIAIRYLETLQRVADGRAT
                     KIFLPLDTSGVLGSVAAIGEMFKETSGSPEG"
     gene            complement(9280..9720)
                     /locus_tag="AC161X_02305"
     CDS             complement(9280..9720)
                     /locus_tag="AC161X_02305"
                     /inference="COORDINATES: protein motif:HMM:NF014062.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR4988413.1"
                     /translation="MDNEVWRWLWTIFAIVMGIGEIFTAGFFLLPFAIGAASAAILAW
                     ADVGVLAQWLVFFGVSLVSLAYLRRFITRQDGAEQPKVGANRWVGVEGIVLEAIDPQS
                     GAGMVKVATEEWRATADQPIPKDARIVVNEVRGARFIVAPVDSE"
     gene            9820..>10003
                     /locus_tag="AC161X_02310"
     CDS             9820..>10003
                     /locus_tag="AC161X_02310"
                     /inference="COORDINATES: protein motif:HMM:NF014862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HIR4988414.1"
                     /translation="MNPEKLTARSREAILAATQAARDHNHGSIQAAHLALALLGQPEG
                     IVYPVLDRLGLRPIDLR"
BASE COUNT         1614 a         3217 c         3341 g         1831 t
ORIGIN      
        1 tcctggtcct caccggcggt cgtcgacggc atgctggtga cagccacctg caacggcgac
       61 gtgcgcggct actccctgga agaccccaga tccccggtga ggcagtggac ggtcgacctc
      121 ggtgagacct gtctcgagtc aacccctgca atctggaacg gggccatcta catcggatcc
      181 cgtgatggct acctcagggc cctcgcccca tgaggcgtcc gatagttctc gtgctgctcg
      241 ctgcgctcac cgcatgcggt tcggcaggcg gtgacgccac caccacctct ccggtcaaca
      301 ccaccacgac ggtcggagag accacgacga ccgagacccc ggtcgattgc ccggcgccac
      361 cgtacgagtt ggagttcctc cccaccggat tgggcaccgg ccttctcgac cccgatgaga
      421 ttgacatgga tgtgtggaca tcggtcggag gcagtcagac cgccttctac ggcaggcagg
      481 acggcacggt cgccatcgcc ctggttcggg ggactttgcc tccagtcgag tggccggcag
      541 aaaagggtga ggtgctccta gacggcactc ggggtgtggt gggaccgcat cctgacggta
      601 cctgggtcgc cggctggtac gaagagcccg gggagcgctg tgacctgtac accatggtct
      661 tctatccccc cgtggctccc accgacgtcg agtccgtgat cgccgggatg aaccgggtcg
      721 gcggttagcg cctcctcttg acgttgcgcc ggtcgcggct taccgtgccg ctcgtatgac
      781 tggaaagacc atctcggcga cctcgaccac ctgggagtgg cgttggcccg cgccggaggt
      841 ggcttcggtc ggatagtccc caggatttct gatcatcaag acccacctcc ggctcggaag
      901 gtgggttctt catttctgag ctcatcttcc tgaccggtga gaggagccct cgttgaacca
      961 ccccttcgac attccagctg acctcgatac gccgacatcg gtgtatctga agctcggccc
     1021 tcttcacccg cgcttcctcc tggagagtgt ggagcacggg gacagggtcg ggcgctactc
     1081 gttcatcggc ttcggcgacg ggctagaggt tcgcagcgac gggcgctcgg tcgacattgg
     1141 cgaaacccgg gtggagaccg atgggacggt gaagggcctg atggatgcgt tgcgcaaggc
     1201 gctcgccgca gcccctcggc ccgggcccct gtcaggatcc gtgccgttcg ccggtggtct
     1261 ggtgggggca gtcgggttcg atgtcgccca tcgcctcgcc ggcgccatcc cggggccttc
     1321 ggcgacgccg gaggtcctgc tctacgggcc gtcgagcgtg atcgtgttcg atcacctcag
     1381 ccgccgggct gccctgctcc acgacggatc ggaggccgag cgcctcgccc tccgtcagga
     1441 ggtgatctcg ctccttgcgg gtggagttcc cgggatcagg aacggtgggg gcagcttcag
     1501 cgagcccttg ccctcgatga agagagacga ctacctcgcg gcagttgagg aggcacggga
     1561 gcacatcgcg gcaggtgatg tatatcaact cgtggtctct gtccgttttg ccggagagtc
     1621 ggatctcgat ccgttcgacg tctatcgagg tcttcgcttg ctcaaccctt cgccgtacat
     1681 gtacttctgt gagctgggcg agacccgggt catcgggtcc tcgccggagg cgctggtgcg
     1741 tctgcggggg accgcggccg aactgcgccc cattgccggt acccggcctc gctccaatga
     1801 cgaggagcac gatcgaaggg ctgaagagga cctgctcgac gatgccaagg aagccgctga
     1861 gcatgtgatg ctcgttgacc tggcccgaaa cgacctgggg agggtggccg tgccggggac
     1921 cattcgggtc gacccctatc gagtcatcga gcgctacagc catgtgatgc acctggtgag
     1981 tggggtcacc gggatcctgg agccgggagc cgacgcattt gacctgttcg cggccgcctt
     2041 cccggccggg accgtggtgg gggcacccaa ggtgcgtgct ctcgagctcc tgcaagagtt
     2101 agaggctgtg ccccggggca tctatgcggg aacggtcggc tacttcgggc atggaggaag
     2161 catggatcag gcgattgcga tcaggactct cgtcatgtca gaaggctcgt attcgtatca
     2221 agccggggct gggatcgtgg ccgacagcgt ccctgccacc gagtgggacg aagttctgtc
     2281 caagagcggt gcgatgcgcg gcgccctgga aagtgcggct gagggcctat gagccgggtc
     2341 ttgctggtcg acaactacga ctcgttcacc tacaacgtcg tgcaggccct ccgaatcctg
     2401 ggagccgagg tgatcgtccg caccaatgac gacatcgatg tcgagtcggc cagagagctg
     2461 gagcccaccc acctcgtcat ctccccgggg ccgggagggc ccgaggatgc aggcgagtcg
     2521 atgaatgtta tctctgggct cctcaccgag gtgccgatcc tgggcgtctg tctgggtcac
     2581 caggcgctcg ccgccgcgct cggcggcaaa gtgcagcgcg ccgatgagct ccgtcacggc
     2641 aaggcctcac ccgtgtatca cgactccgca accatctacg ccggccttcc caatccgttc
     2701 gatgccggcc gataccactc gctggcggtc caggaagagg cacttcccag cgagctcgta
     2761 gtcacttcct tcacctcgga cggggtgatc atgggggttc ggcatcgtga gtatccggtg
     2821 gagggtgtgc agttccaccc cgagagtgtc cttactccgc acggtgacct tctcctccgc
     2881 aacttcttgg atatgacagg gacgaagtca tgagagagtt gcttgacgac ctgttggcgg
     2941 ggaccgacct atccactcca gccgcggatg aactgttggt gaacctgacc agcggcaatg
     3001 tggagccggc aatggccggc gcgattctcg ccgccttgcg ggcgaaaggt gagaccgccg
     3061 acgaggtccg aggttttgcc tcggcgatga gaaggttgtc cagcgatccg gggattgatg
     3121 cgtctggcgc ggtggacgtc gtgggaacgg gaggggatcg gtccgggtcg ctcaacctgt
     3181 cgacgggggc agcacttctc accgcggcca gcggtgtgcc ggtggtcaaa catgggaaca
     3241 ggtcgatgac cagtcaatcc gggagcgcgg acatcctcga ggcgatcggt ctccccatcc
     3301 cgctcgatgc cgaccaagcg aggaggtgct ttgagactgt tggcttcacc tacctcttcg
     3361 ccccccacta tcaccccgcc atggccgcag tgggcccgat tcggaaggct ttgggggtcc
     3421 gaacggtgtt caacatgctc ggcccgttga ccaaccccgc cgggccccgc cacctcgtcg
     3481 tgggcgcctt tgacgccagg gccgctgagt tgatggccgg agccctctcc ggtctcgaga
     3541 tcgaccgcgc cttcgtcctc cacggtgcgg ccgggtggga tgagccgagc ccgatcggcg
     3601 agttcctcct ctacgacgtg caaccgggct cggtcgtccg cagcacccgg gacccggtcg
     3661 accttgggat gcagcggtgt gaccccaggg agttggcagg aggtgatccc gcccacaatg
     3721 ccgcccaact caccgcggtg ttctcgggag aaagcgggcc ccaccgtgac tcgatcctcc
     3781 tcggcgccgc cctcgcactg gaagtcaccg ggcgcgccac caatccggcc gatgcagtgg
     3841 aagtggcgcg gagcgccctg gacgatgggc gggcaatggc cctgcttcag ggactgcagc
     3901 tattcggggc aggtgagagg gcgtgagcag gttcctccag tcgatggagc agtcctcgcg
     3961 gtcccggctc gagctcgccc ggagtcggcg ctcgctcggg gcgatcacag cctccgctct
     4021 cgacgccccc tcggttcgcg acgtcgggac cttcggcgag gtgttcgatc tgattgccga
     4081 gatcaagcct cgatctcccg ccgaaggtga gcttgccggt tccgaactgg tggaaagggc
     4141 catcgcatac caggagggag gggcacgaat gctctcggtg ctcaccgagc ccgaggcgtt
     4201 cggcggctct cttgctctgc tcgaggcggt ggccgcggtt gcccgggtcc ccgtgctccg
     4261 caaggatttc ctggtagacc cctatcaggt cttcgagtcg cgagccttcg gtgcagattg
     4321 cgtcctcctc atcgcacgtc tgctgtccga ccaggcgatg gccgacctgc tggacgccgt
     4381 ggaggagacc gggatggtcg ccctcctcga ggcgttcgac gcaaccgagc tgagccgggt
     4441 gaactccatc gctgaaactt cgcctggcac gttggtagga gtcaactgca gggacctgga
     4501 gacacttcac accgtcccag agcgtcacga cgaacttgca cctggtctct cactgaccat
     4561 tccctcggtt gcagagagcg ggatcctggg aacggacgac gcccgccggg tggccggcct
     4621 cggataccgc gccgctctgg tcggctcggc cctgatgaga tcggacgatc cggctgactt
     4681 ggcccgaacg atgctcgccg ccggccagga gagcgcggcg gtcgcgtcat gagcctcttc
     4741 atcaagcttt gcggcctgaa gaccgacgcc gacgtcgaga ccgccgtcga ggccggggcc
     4801 gatgcggtgg ggttcgtgct caccccatcc tcacgtcaga ttgccctgtt cgaggctgcc
     4861 aagctgcggc gacttctgcc ggagtcggtg cttgcagtgg ccgtcttcaa cgacccaagt
     4921 ccgaacctga ttgagcaagc actcgaggag gtagccccag atctggtgca ggttgaggca
     4981 cgcaacggga ccggggtccc aattcagaag ttcctccctg tggtgctcaa tggcaatgga
     5041 gcgccagagg cagtcatgca ggcgctggag ttgacggagt cggggatggt tctcgttgac
     5101 accgccgaga agggcggcac tggccgacgg tccgattggg gacgcctcgc ccggctgaag
     5161 gtgcgggatc gggtcatcgt cgccggcggt ctcaaccccg ggaacgtcgg ggaggcaatc
     5221 tcgacgattg gcccgctcgg ggtagatgtg agcaccggca tcgagcgccg gccaggtgag
     5281 aaggacccag tgttgatgag gcagttcgtg agagcggcgc gcgctgccga agtgagagga
     5341 gcgagtcgat gagcaccgca ggcagatttg gcagcttcgg tgggagctat gtccccgaaa
     5401 tactggtgcc agccctcacc aggctggagg agaccgcgac ccgactcctc gccgatccgg
     5461 gctttctcgc gatgtacgaa accgaattgt cgagttgggt gggcaggccg acggcgctga
     5521 cccgggctgc ccggttgggt gaggcctggg gtggcgagat ctggctgaag cgggaagatc
     5581 tggctcatac cggcgcccac aagatcaata acgcgatcgg ccaggcactg ctggccaggg
     5641 aaatgggagc ggggcgagtg atcgccgaga ccggggcagg tcagcacggt gttgccaccg
     5701 cggccgcttg cgcccgtctc ggcctcgact gcaccgtcta catgggcacg gtggacatcg
     5761 agcgccaagc tccaaacgtg gagcggatga agctgctcgg cgccaaggtc tccccggtgg
     5821 tctctggtga tgccaccttg cgggccgcca tcaacgaagc cctccgtgat tgggtcaccg
     5881 acccgaacag cacctactac ttgctcggct cggcggtggg gccccatccc tacccatggc
     5941 tggtccgaga gctgcagtcg gtcatcgggc gtgaggcgcg ccagcagatg ctggatcggg
     6001 ccggccggct ccctgatgtc gcctttgcct gtgtcggcgg cggatccaac gccatcggcc
     6061 tcttccatcc cttcctcgac gatccagtgc agctgatcgg cgtcgaggcg ggcggccgcg
     6121 gccccggtct cggggagaac gccgccacca tcgggcgtgg cagcccgggc gtcctccatg
     6181 gcagttactc attcatactt caggacggcg acgggcagat ccaggagacg cattcggtct
     6241 ccgccgggct cgactacccc ggtgtcggcc cggagcacgc ctggctcgct gagaccggtc
     6301 gcgccagcta tgtggaagcc tccgatgacg aggcgctgtc cgccttggac gaactgggac
     6361 gtctcgaagg catcctgccc gctctcgagt ccagccatgc actcgccgcg gtgcgcaggt
     6421 ggctgccagc gacaccgggc gccgtggtgc tcgtcggact gtccggtcgc ggagacaagg
     6481 acatcgacat tctccggggg ctcaaccgat gaccaccgca ctcacaggtg ccgggcttct
     6541 cgagcagcgg ctgagggccg atgctcctgc cgtggttccg tatctgactg ccgggttccc
     6601 caccatcgaa cgattcgcag acgccttggc cgcggtcgcc gcgatctcgg aggccgtcga
     6661 aatcgggatt cccttcagcg atccgatggc cgacggagcg acgattcaag aatcgagccg
     6721 aatcgcactg gaagccgggg ccaccctgga gttggttatc tcggcgctcg agtcaatcgg
     6781 ggccctccca tgccccctcg tcgtcatgag ctacttgaat ccgctgatgg cattcggctt
     6841 cgaccggctc ctgccgcgac tttcgggcgt tggtgttgct ggcctggtgg tcccagatct
     6901 cccgttggat gaatccgaag atctcagggg cgtcgccgct gagcacggcg tcggacttgt
     6961 gcaactggtg actccggtaa ccgaggacga tcggctgcga cgtctcggcg aggcctccga
     7021 aggattcacc tatgccgtga ccatgaccgg gaccaccggc ggctcggccg gggtgggatc
     7081 cgatgtcgcg ggctatctgg acagggtgcg cggggtgtcc cgggccccgg tgctggcagg
     7141 attcggggtc agaagctccg atcaggttcg ctccctggta ccccattgcg acggggtcat
     7201 cgtcggcagc gcgttggtcg aggtcttggg ccgcgaagag gatccggtgg cattcctgac
     7261 gggcctccgg ccatgatcgc caccctcacc gccgactgca cactggcgca gaaggccgcc
     7321 attctccgct tcatcgaggg ctacgggttg cgagtccacg tcggcgaggc cggatcgacg
     7381 accgtggtgt cggtcatcgg tgactatgag cccgggatgg cggagtcgat cggcgctctg
     7441 ctcggtgtcg actcggtgag caccgaggct cctctctacc cgttcgtggc gcgggcggcg
     7501 cgcaactcgc cgacgaccgt ccgcgtcggt gatgtggtcg ttggcggccc tgagatcgtg
     7561 gtcatcgccg ggccttgctc ggtggagtcg gcggcccaga tcgaccggat cgccaccggg
     7621 gtcgccaaag agggtgccgt cctgttgcgt ggcggtgcct tcaaaccacg gtcctccccg
     7681 tacagctttc aggggatggg tgaggacggc ctggaactgc tggcggctgc ccgtgagtcg
     7741 acaggcttgg gtgtggtgtc cgagtgtgtc gctccggagg acgtgccctt gctcaaccgg
     7801 tatgtcgaca tgctgcaggt gggaagtcgc aacatgcaga acttccggct cctctcggcg
     7861 gtgggtgagc aacccaagcc ggtgctattg aaacgggggc tgatgtcgac catcgaggag
     7921 ctgttgttgg ccgccgagta catcgtgggc tcgggcaacc cgcgggtgat cctgtgtgag
     7981 cgtggggttc gctcgttcga cactgccacc aggaacatcc tcgacatcag cgcggtcccg
     8041 gtgctcaagg agcggaccca cctcccggtg atcatcgacc ccagccatgc cgccggccgc
     8101 cgctctctgg tgcccagcct cgctgccgcc gggatcgctg ccggcgccga cgggctaatc
     8161 gtcgaagttc acgatcgacc agaggaggcg ctcagcgacg ggccgcagag cctgaccctg
     8221 gagggattcg ccgagacgat ggggacggtg cgccgggtgg ctgccgcact cgatcgaacc
     8281 gtttccacgc ttggcgtagt acgtactacg taggacgtac tacgaggatc acccttcggg
     8341 tgatcccgaa gtctccttga acatctctcc gattgcggcc acgctcccca ggaccccgct
     8401 ggtatcgagg ggtaggaaga tcttggtcgc ccgcccgtcc gcaacccgtt gcagggtctc
     8461 cagatagcgg atcgctatca ggtcgggcga cgggttccca ttgtggatcg cggcgtaaac
     8521 ccgttcgatg gcttgcccct caccttcggc cacggtcagc ttctggtact tctcggcgtc
     8581 ggcgacccgc ttgatcgcct cggcctgacc atctgcctcg agcacctggc tctccttgag
     8641 gccctcggcc ttgaggatcg cggcccgctt gaggccctcc gcctcggtga ccgcggctcg
     8701 ccggtcccgc tcagccttca tctgacggtg catggcatcg gtcacgtcgg tcggcggctc
     8761 gatgcgctgg atctcaacgc gaaccacccg gactccccat ttgtcggtgg cgtcgtcgag
     8821 gatctggcgc agcttggcgt tgatcacctc gcgggaggtg agcgcggagt cgaggtcgag
     8881 atcaccgaca acgttcctga ggttggtctg ggccaacttg gtgaccgcca gcatgaaatt
     8941 gccgacgttg tactcgagct tcaccgggtc ggtggcctcg tagtagatga cggcgtcgac
     9001 ggtgacgacc acgttgtcct tggtgatcac ctcttgggga ggcacgtcga tcacccgctc
     9061 ccgcatgtcg accttggtca aacgcttcac gaagggcagg acccagcgca ggccggagtc
     9121 gacggtgcgc tggtagcggc caagaaactc gatgaggccc ttttcgtacg gcctgaccac
     9181 cttgaatccg gccgcggcga tgaggatcaa caccagcgcc atgacggcga acaggattat
     9241 cactgcctcc atgtctcttc ctttcctagt cgtccaaagt cattcgctgt ccaccggtgc
     9301 gacgatgaac ctcgcccccc gaacctcatt gaccacgatc cgggcgtcct tggggatcgg
     9361 ttggtccgcg gtggcccgcc actcctcggt ggcaactttg accattcccg ccccgctctg
     9421 gggatcgatc gcctcgagga cgatgccctc gacacccacc cagcggttgg cgccgacctt
     9481 gggctgctcg gccccgtctt gacgcgtgat gaaacgtcgc aggtaggcga gggagacgag
     9541 ggagacgccg aaaaacacca gccactgggc gaggacgccg acgtctgccc aggcgaggat
     9601 ggcggccgag gcggccccga tggcgaaggg caggaggaag aaaccggcgg tgaagatctc
     9661 gccgatcccc atgacgatgg cgaagatcgt ccacaaccaa cgccagacct cgttgtccac
     9721 gtcgtctacc tcctgacgct cagggctgtg gtgtcgttca ccacgcccat caaatcaccc
     9781 acaccttatg attctgtggc tgtccgcgag ttgtgaggaa tgaatccgga gaagctcacc
     9841 gcccgcagcc gggaagcaat cctggctgca actcaggccg cccgggatca caaccatggg
     9901 tcgattcagg cggctcacct tgcgctggca ttgctcggcc agcccgaagg gatcgtctat
     9961 ccggtcctcg accgtctcgg cctgcggccg atcgacctgc gcc
//
LOCUS       DBNHXU010000087         4772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_226206, whole genome shotgun sequence.
ACCESSION   DBNHXU010000087 DBNHXU010000000
VERSION     DBNHXU010000087.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4772
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_226206"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC161X_02315"
     CDS             complement(<1..308)
                     /locus_tag="AC161X_02315"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4988415.1"
                     /translation="MAGTSNAITVEGLVKHYKKTKALDGLDFTIPSGAITGFLGPNGA
                     GKTTTFRSLLGLTRPNSGSVEVLGHKVPSELKAVTKQVGAIIEEPGLIRALGGRVNLL
                     V"
     gene            complement(342..770)
                     /locus_tag="AC161X_02320"
     CDS             complement(342..770)
                     /locus_tag="AC161X_02320"
                     /inference="COORDINATES: protein motif:HMM:NF014119.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HIR4988416.1"
                     /translation="MGLGHVSSRPLPATRESSHFPRRERRGTGTARAVSLNDTGAVGR
                     LGESLARRFLTDHGLSIVASNVRVEGGEIDIVASDHGRTVAVEVRAITGEGDPIDAID
                     AGKRRRVRRLAHQAGATRVDFVGIRIETGAAVVHWVPGGV"
     gene            complement(662..958)
                     /locus_tag="AC161X_02325"
     CDS             complement(662..958)
                     /locus_tag="AC161X_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007075222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2469 family protein"
                     /protein_id="HIR4988417.1"
                     /translation="MEEDLERYESEVELKIYKEYHDVLPMFRYVVETERRFYLANEVD
                     LQVRDANGSIFFEVEMRDAWVWDMFRPARFLQHVKVLTFRDVNVEELEQQELSL"
     gene            complement(1034..1576)
                     /gene="lepB"
                     /locus_tag="AC161X_02330"
     CDS             complement(1034..1576)
                     /gene="lepB"
                     /locus_tag="AC161X_02330"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR4988418.1"
                     /translation="MTALAIAVIIKTFLIQPFWIPSGSMEPTIEINDRVMVNKLAYKW
                     GEPQRGDIVVFRDPREDEIEESIPEAVIRSVLEAVGVRTRGRDDLIKRVIGLPGDTIE
                     IRANHIVVNGTPLDEAYLEEDLEMPDEGPFPVGEGEVFVMGDNRNASFDSRRFGPVPL
                     EDLVGQAFVTIWPLAHFGAF"
     gene            1491..1691
                     /locus_tag="AC161X_02335"
     CDS             1491..1691
                     /locus_tag="AC161X_02335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988419.1"
                     /translation="MVGSIEPDGIQNGWMRKVLMMTAMARAVRRRPGSSARNDRFFSG
                     AGLSSGAPSTWFGVGRVSRAAP"
     gene            complement(1672..2016)
                     /gene="rplS"
                     /locus_tag="AC161X_02340"
     CDS             complement(1672..2016)
                     /gene="rplS"
                     /locus_tag="AC161X_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006237373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HIR4988420.1"
                     /translation="MNDLHLVESAYVRDDIPEFRPGDTVKVHVRVMEAGRERIQVFEG
                     VVIARNRTGARASFTVRKISFGIGVERVFPVNAPIISKIEVARRGDVRRSKLYYLRDR
                     VGKSARIKELRD"
     gene            complement(2077..2760)
                     /gene="trmD"
                     /locus_tag="AC161X_02345"
     CDS             complement(2077..2760)
                     /gene="trmD"
                     /locus_tag="AC161X_02345"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012833895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HIR4988421.1"
                     /translation="MRINVVTIFPDYFESPLRVSLVARALEAGALEVELIDLREHGLG
                     RHRQVDDAPFGGGAGMVMMIEPLAAALEPLTGSHRVLFTPVGHPMTQATLDDWAGREE
                     VTFVCGRYEGVDQRVADNLIDEEVSLGDFVLAGGEVAAAASIEGIARLLPGVVGNPDS
                     TETESFRHGLLEEPVYTRPAEFNGWPVPEILLSGDHGKVDEWRSQQRIERTRARRPDL
                     LADGDKSET"
     gene            complement(2785..3948)
                     /locus_tag="AC161X_02350"
     CDS             complement(2785..3948)
                     /locus_tag="AC161X_02350"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013151140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HIR4988422.1"
                     /translation="MNEAVIVATARSPIGRAYKGSLIEVRADDLAGFAINALMDKVPQ
                     VDRASVFDVMLGCGLTHHEQGYNLARPASLLAGMPESVPATTVNRFCASSLETIRMAF
                     HAIRSGEGDTYVAAGSESTSRAYGKAFDSEDLNPRFTDPDRPDFIDQVYIPMGNTAEN
                     VAERYGITRERMDEFAKRSQDLAVESQKNGFFDWEISPIDLPDGRTISADDGPRPNTT
                     IEKLAELAPVFKQGGTVTAGNSCPLNDGSAAVLVMSADRAGQLGLDPLARIVASSVVG
                     LEPEFMGMGPVGAVRSVLAQAGMSLADIDVIEINEAFAAQVLACADELDIDIEKLNPH
                     GGSIALGHPFGMTGARIMGTLINGLRNRDQAIGLETMCVGGGQGMAMIIERLS"
     gene            3988..>4772
                     /locus_tag="AC161X_02355"
     CDS             3988..>4772
                     /locus_tag="AC161X_02355"
                     /inference="COORDINATES: protein motif:HMM:NF022114.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HIR4988423.1"
                     /translation="MRSPALAGLLLLAACLPTTAATTTSIAAPATTTTTVGAPTTVAI
                     TEPVLGCADASDFLESGQVVRIDQPSTDASILGLISWDVTGGCERFRLDFETNEGAPA
                     TTPPSVAVEFLDSGQILRIHLDLDRTVLTDQLVETPLVDRLYVVRSLDGGLFVDLHLA
                     GPSQARAEVRNSPSGLTVELQMGIQPFTGAVTIADQVVVVEPSQGAETGTNFEVSGYA
                     RTFEGTVVVIANPAGAGTGGGNSTQAADWAETWGEFQTTIEVLP"
BASE COUNT          883 a         1532 c         1469 g          888 t
ORIGIN      
        1 accaggaggt tgacccgccc tccgagggcg cgaatcagcc cgggctcctc gatgatggct
       61 cccacctgct tggtgacggc cttcaactcg gaaggcacct tgtggccgag gacctcaaca
      121 ctcccgctgt tgggccgggt caggccgaga agcgagcgaa acgtcgtggt cttccccgcc
      181 ccattcgggc cgaggaatcc ggtgatcgca cctgacggga tggtgaagtc gagaccgtcg
      241 agggctttgg tcttcttgta atgcttgacc agtccttcga ctgtgatcgc gttggaggtg
      301 ccggccattg cgtccaagct agggaagccc gaaaacgagt gtcaaactcc gccaggcacc
      361 caatggacca cggcggcccc ggtttcgatt ctgatcccga cgaagtcgac cctggtcgca
      421 cccgcttggt gggccaaacg acgaaccctt cgtcgtttcc cggcgtcgat ggcgtcgatc
      481 ggatcgcctt ccccggtgat ggcccggact tccacggcga ctgtccgtcc atggtcagaa
      541 gccacgatgt cgatctcgcc accttcgacg cgcacgttgc tggcgacgat cgaaagaccg
      601 tgatcggtga ggaacctgcg ggcgaggctc tcacccaggc gcccgactgc accggtgtcg
      661 ttcagagaga cagctcttgc tgttccagtt cctcgacgtt cacgtcgcgg aaagtgagaa
      721 ctttcacgtg ttgcaggaag cgggcgggac gaaacatgtc ccaaacccat gcgtcgcgca
      781 tctccacctc gaagaagatc gagccgttgg catcgcgcac ttgaaggtct acttcgttag
      841 cgaggtagaa gcgacgctcg gtctccacga cataccgaaa catcggcagg acgtcgtggt
      901 actccttgta gatcttgagc tctacctcgg actcgtagcg ctccaggtcc tcttccatgc
      961 actcctcttc acacgctgct ggcgatcata tgtcgccaag agaatctaaa catccaccaa
     1021 ggacagggtt ttttcagaag gcgccaaaat gtgccagcgg ccagatggtc acgaatgcct
     1081 gcccgacgag atcctccagc ggcaccgggc cgaagcgacg gctgtcgaaa gaggcgtttc
     1141 ggttgtcccc catcacgaag acctccccct caccgaccgg gaatggcccc tcgtcaggca
     1201 tctcgagatc ttcttccaga tatgcctcgt cgagtggggt cccattgacg acgatgtggt
     1261 tggccctgat ttcgattgtg tcgccaggaa ggccgatgac cctcttgatc agatcgtccc
     1321 ggccccgggt gcgcaccccg accgcttcga gcaccgatcg aatgaccgcc tcagggattg
     1381 actcctcgat ctcgtcctcg cgcggatcgc gaaaaacgac gatgtcgccg cgttgcggct
     1441 caccccactt gtaggcgagc ttgttcacca tcacccgatc attgatctcg atggtgggct
     1501 ccatcgagcc cgacgggatc cagaacggct ggatgaggaa ggtcttgatg atgaccgcga
     1561 tggcgagagc ggtcagaagg aggccgggga gctcggcgag gaatgatcgc ttcttctccg
     1621 gcgccgggct ctcctcgggg gcgccttcta cctggtttgg cgtgggcagg gtcagtcgcg
     1681 cagctccttg atccgggcgg acttcccgac ccggtcacgg aggtaataga gcttcgaccg
     1741 gcggacatca ccgcggcgag ccacttcgat ctttgagatg atcggcgcgt tcactggaaa
     1801 caccctctcg actccgatgc cgaagctgat cttgcgaacc gtgaacgagg cacgggcgcc
     1861 ggttcggttg cgggcgatga ccacaccttc gaagacctgg atcctctccc ggccggcttc
     1921 catgacccgc acatggacct tgacggtgtc gccgggcctg aattcgggga tgtcgtcgcg
     1981 gacatacgcg ctctcgacaa ggtgaagatc gttcattggg agactcctag gagcggtcgg
     2041 aaagggtctg ataatcccga cccggcagaa attgtatcag gtctccgatt tgtcgccatc
     2101 tgccaaaagg tccgggcgac gagccctggt ccgctcgatg cgctgttggg agcgccactc
     2161 atccaccttg ccgtggtcac ccgacaggag gatctcgggc accggccatc cgttgaactc
     2221 agcagggcgg gtgtagaccg gctcctctag caaaccgtgg cgaaatgact cggtctccgt
     2281 cgaatccgga ttgccgacca ccccgggaag gagtcgagcg attccctcga tcgatgcggc
     2341 ggcggcaacc tcgcctccgg caaggacgaa gtctcccaac gagacctcct cgtcgatcag
     2401 attgtcggcc accctctggt cgaccccttc gtaacggccg caaacgaaag tgacctcctc
     2461 gcggccggcc cagtcgtcga gggtggcctg ggtcattgga tgcccgacag gagtgaagag
     2521 cacacggtgc gaaccagtga gtggctcgag ggcggccgca agcggctcga tcatcatcac
     2581 catgcccgct ccgcctccga aaggagcgtc atcgacctga cggtgcctgc caagaccgtg
     2641 ctcccgcaag tcgatcagtt caacctcgag ggcacccgcc tcgagggccc tggccaccag
     2701 gctgacccgc aacgggctct cgaagtagtc cggaaagatg gtgacgacgt tgatccgcac
     2761 gatgcgggaa gggtagggcg gggtctagct gagccgctcg ataatcatcg ccatcccctg
     2821 cccaccgccg acgcacattg tctccagacc gatggcctgg tcgcggtttc gcaaaccgtt
     2881 gatgagggtg cccatgatcc tggccccggt catcccgaac gggtgcccga gggcgatcga
     2941 gcccccgtga ggattgagct tctcgatgtc gatgtccagc tcatcggcac aggccaatac
     3001 ctgggcggcg aacgcctcgt tgatctcgat cacatcgatg tcggccaggc tcatcccggc
     3061 ctgggcgagg accgagcgca ccgcaccgac cgggcccatg cccatgaact cgggctcgag
     3121 cccgaccacc gaagaggcaa cgatccgagc cagcgggtcc agacccagtt ggccggcccg
     3181 atcggccgac atcaccagga cggctgcaga accgtcattg agagggcaag agttgccggc
     3241 ggtgaccgtt ccgccctgct tgaacaccgg ggccaactcg gccagtttct cgatcgtggt
     3301 gttcggccgg gggccgtcgt ccgccgagat ggtccggccg tccgggaggt cgattgggct
     3361 gatctcccag tcgaagaagc cgttcttttg agactccaca gccaggtcct gggatcgctt
     3421 ggcgaactcg tccatccgct cccgggtgat cccgtagcgc tctgcgacgt tctcggcggt
     3481 gttgcccatc gggatgtaaa cctggtcgat gaagtcgggc cgatcagggt cggtgaatcg
     3541 gggattcagg tcctcgctgt cgaaggcctt tccgtacgcc cgacttgttg actcggatcc
     3601 cgcggcgaca taggtgtcac cctcccctga ccggatggcg tgaaaggcca tccggatcgt
     3661 ctccaaagag gaggcgcaga agcggttgac agtggtggca gggactgatt caggcattcc
     3721 ggccagcaag gaggcgggtc gggcgaggtt gtatccctgc tcgtggtggg tcaggccaca
     3781 ccccagcatg acgtcaaaca cagaggcacg gtcgacctgc ggcaccttgt ccatcaaggc
     3841 gttgatggcg aaacccgcca ggtcgtcggc cctgacctcg atgagggagc ccttgtaggc
     3901 gcgaccgatc gggctacgag ccgtggcaac aatcactgct tcgttgatca cacgtttcaa
     3961 tgtaccgccg gacgtaccct gacggatatg cggtctccgg cgctggcagg tttgttattg
     4021 cttgctgcat gcctacccac cacagctgcc accaccacgt cgatcgcggc tcccgccacc
     4081 acgactacga ctgtcggtgc tccgacgacg gtagcgatca ccgagccggt tctgggctgc
     4141 gccgatgctt cggacttcct cgagagcggc caggtcgtcc ggatcgacca gccttccacc
     4201 gacgccagca tcctcggcct catctcctgg gacgtcaccg gtggctgcga gcgtttccgc
     4261 ctcgatttcg agaccaacga aggtgccccg gccaccaccc cgccctcggt ggcagtcgag
     4321 ttcctcgact cagggcagat actccgcatc cacctcgacc tggaccgaac cgtcctcacc
     4381 gaccagttgg tggagacacc actggtagat cggctctatg tggtgcggtc actcgatggg
     4441 gggttgttcg tcgacctcca tctcgccggg ccaagtcagg cccgcgccga ggtgagaaac
     4501 tctccgtcgg gtctcaccgt cgaactgcag atgggaatcc agcccttcac cggtgcggtg
     4561 acaatcgctg atcaagtcgt ggtggtcgag ccttcacagg gtgcggagac aggcaccaat
     4621 ttcgaggtca gcggttacgc gcggaccttc gaaggcactg tggttgtgat cgcgaacccc
     4681 gccggagcgg gcaccggcgg gggcaacagc actcaagcag cggattgggc cgagacctgg
     4741 ggcgaattcc agacgacgat cgaagtcctg cc
//
LOCUS       DBNHXU010000088         2457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3797751, whole genome shotgun sequence.
ACCESSION   DBNHXU010000088 DBNHXU010000000
VERSION     DBNHXU010000088.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2457
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3797751"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /locus_tag="AC161X_02360"
     CDS             complement(<1..325)
                     /locus_tag="AC161X_02360"
                     /inference="COORDINATES: protein motif:HMM:NF012780.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HIR4988424.1"
                     /translation="MTVRELVGGEVIWVAPEATMRSAAVSMKSNGVGSVAVEVDGALE
                     GILTERDLLDAVADGADLDLERVDRWMTSYPDSIDPDMQVTEAAQWMLASGYRHIPVI
                     DGSRIL"
     gene            452..1825
                     /locus_tag="AC161X_02365"
     CDS             452..1825
                     /locus_tag="AC161X_02365"
                     /inference="COORDINATES: protein motif:HMM:NF019352.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HIR4988425.1"
                     /translation="MTPRLHEPEDQPTKLVIDRLLVLVAVMGAAFAIMAAVIAIRGGD
                     TAGAATGDEQQSGHVSLSEFSIDGDLAFAPGSVALHVSNDGSQIHNLTVEGGTTTPDI
                     SAGDSAQLDLDDLTEGTYVVFCSIPGHRDAGMEATLTVGTGATSAEGEGQHTAADWAK
                     MDQMMTDSMLAFPAATEGKGNQLLEPTVLADGTKEFHLTAAVTKWEVAPGKIVDAWTY
                     NGMVPAPMIRVGVGDRVRVVLDNQLPMGTDIHWHGVKVPNSMDGVSPITQPLVKPGET
                     FVYEFTATEPAIAMYHAHHHAQMQVPNGLFGTFIVGDIPVPRGQTISGVEIPTELEIA
                     QEIPLVLNDAGVIGLTLNAKSFPATEPYTGKVGDWVVVHYYNEGLQIHPMHQHQFPQL
                     VFAKDGIPLDHPYWADTVAIAPGERYSVLFQLSDPGVWVWHCHILNHVESDTGMFGMV
                     TAWIVEE"
     gene            1844..>2457
                     /locus_tag="AC161X_02370"
     CDS             1844..>2457
                     /locus_tag="AC161X_02370"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR4988426.1"
                     /translation="MFNSIYPETGAVDDLLGLVLGSERSIVGRPWVMLNMVASIDGAT
                     SVNGRSRGLADDDDRTMFKALRAVPDIILVGAETARADDYGPIRLDEERIQRRRAVGK
                     PDLPRLAVLSGHLNLDPGAKLFSDPDLRPIVLTGRDADSDRLKSLEPIAEVVVFEDLS
                     IESVLGHFGSGVILCEGGPGINAQLAEVGAIDELNLTVAPLIVGG"
BASE COUNT          479 a          775 c          749 g          454 t
ORIGIN      
        1 ccaggatccg ggacccgtcg atcacaggaa tgtgccgata gccgctggcg agcatccact
       61 gcgccgcctc ggttacctgc atgtccggat cgatcgaatc cgggtacgac gtcatccatc
      121 ggtcgactct ttccagatcc aggtccgcac cgtcggcgac agcgtcaagg aggtcacgct
      181 cggtgaggat cccttcaagg gcaccatcga cttcgacggc caccgagccc acgccgttgc
      241 tcttcatgct gaccgcggcg cttcgcatcg tggcttccgg cgccacccat atgacctccc
      301 caccaaccaa ctcacgaact gtcatcagct gcctttctct gcttgtagcc atcttcgggc
      361 gagcgaggcg ggcgggggag cggcattggt cccaagttga agggacctat gcgcctaggg
      421 agccactggt cccatttctt acctttgccc gatgacacca cgacttcatg aaccagaaga
      481 ccagccgacc aagctcgtga tcgaccgtct gcttgtcctg gtcgcggtca tgggggcggc
      541 gttcgcgatc atggccgcgg tgatcgcgat ccggggcggc gacaccgcgg gggcagcaac
      601 cggcgacgaa caacagtccg gtcacgtcag cctcagcgag ttctccatcg atggtgacct
      661 cgcattcgca ccgggatcag tagccctgca tgtatcgaat gacggttcgc agatccacaa
      721 cctcaccgtc gaaggcggga ctacaacccc cgatatcagc gctggcgact cggcccagct
      781 cgatctagac gacctgactg aaggtaccta tgtggtcttc tgctcgattc cgggccatcg
      841 cgacgccggc atggaggcca cgctgactgt tggcactggc gctacgagcg cggagggtga
      901 gggtcagcac accgcagctg actgggcgaa gatggaccaa atgatgaccg actcgatgct
      961 ggcctttcct gccgccaccg agggaaaggg caatcagctt ctcgagccca ccgtgctcgc
     1021 cgacggcacg aaggagttcc acctcaccgc ggccgtcacc aagtgggagg tcgctccggg
     1081 gaagatcgtt gacgcatgga catacaacgg catggtcccg gcaccgatga tccgggtggg
     1141 cgtaggcgac agggttcgag ttgtcctcga caaccaactg ccgatgggaa ccgacatcca
     1201 ctggcatggg gtaaaagtcc ccaacagcat ggacggggtg tcaccgatca cccagcccct
     1261 ggtgaagccg ggggagacct tcgtctacga attcacggct accgagcccg ccatcgccat
     1321 gtaccacgcc caccatcacg cacagatgca ggttccaaac gggttgttcg gcaccttcat
     1381 cgtgggagac attccggttc cgaggggtca gacgatctcc ggggtcgaga ttccgacaga
     1441 actggagatt gcccaggaga tcccgctcgt tctcaacgac gccggtgtga tcgggctcac
     1501 tctcaacgcc aagtcatttc cggcgaccga gccctatacc ggcaaagtcg gggattgggt
     1561 ggtggtccac tactacaacg aggggctgca gatccacccc atgcatcaac accagttccc
     1621 acagttggtg ttcgccaaag acgggatccc gctcgatcat ccgtattggg ccgacacggt
     1681 ggccattgcc ccaggcgagc gctactccgt cctcttccag ctttccgacc cgggggtgtg
     1741 ggtctggcac tgccacatcc tcaatcacgt cgagagtgac accggcatgt tcgggatggt
     1801 tacggcgtgg atcgtcgagg agtgatcgct acctttcgaa gcgatgttca acagcatcta
     1861 tccggagacc ggcgccgttg acgacctcct gggcctggtt cttgggtcgg aacgctccat
     1921 cgtgggccgc ccctgggtga tgttgaacat ggtggcttca atcgatggag ccacctcggt
     1981 gaacggtagg tcacgcggac tggcggacga cgacgacagg acgatgttca aggcgctacg
     2041 cgcggttccc gacatcatcc tggtgggtgc ggaaacagcg cgggccgacg attacgggcc
     2101 gatccgtctc gatgaggaac gaatccagcg ccgaagagcg gtgggcaaac ctgacctgcc
     2161 caggttggcg gtcctgagcg ggcatctcaa cctcgatcca ggggcgaagc tcttctccga
     2221 cccggatcta cggcccatcg tcctcaccgg gagagatgca gactccgatc gcctgaaatc
     2281 cctcgaaccg atcgccgagg tcgtggtgtt cgaagatctg agtatcgaat ccgtgctggg
     2341 tcatttcggg tccggggtca tcctctgcga aggagggccc ggcataaacg cacagcttgc
     2401 ggaggtcggc gcaatcgacg agttgaacct gacggttgcg cccctgatcg tcggtgg
//
LOCUS       DBNHXU010000089         3579 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2407940, whole genome shotgun sequence.
ACCESSION   DBNHXU010000089 DBNHXU010000000
VERSION     DBNHXU010000089.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3579)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3579)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3579
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2407940"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..445)
                     /locus_tag="AC161X_02375"
     CDS             complement(<1..445)
                     /locus_tag="AC161X_02375"
                     /inference="COORDINATES: protein motif:HMM:NF023818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3662 domain-containing protein"
                     /protein_id="HIR4988427.1"
                     /translation="MNLARSLERRLERLFEGAAGRVFSGRIHPSEMAGRIAREADLTS
                     YEHITGPATSNQYVLKVNPIDLDENPTRLQKRLELALSEHAAEAGLRLEGPPTVLIEA
                     SPSVSPGHFTCVPSVVPGKIAPWGRLVGAEASYDLGPNRVLIGRSS"
     gene            530..613
                     /locus_tag="AC161X_02380"
     tRNA            530..613
                     /locus_tag="AC161X_02380"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:564..566,aa:Leu,seq:cag)
     gene            complement(641..1888)
                     /locus_tag="AC161X_02385"
     CDS             complement(641..1888)
                     /locus_tag="AC161X_02385"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4988428.1"
                     /translation="MCDNMPPMPKSLWRNRDFLLVLGGGFVNNVGDWLLAVALPAFVY
                     TQTGSGRATATIVVTELVIGVVFGPYGGSLADRWDLRRTIIATNVAQAAGMLPLLAVT
                     PDRVWPAFLVAGIQGLLAQINNPASFAIVPRIVPSEQLVTANSANSAGQSIARLLGAP
                     LGGIAVALGGLSTVVVVDAITFLTVAVATTLVRTPTASLTTSDGTDRNEAGVRAGLRE
                     LGLHAGLKGYLGAETVAALTFAMFPVLFIAFVVDVLNGDEATVGIIRGSAAFGGIAAA
                     VMVGRLGNRADPTRLMMWGYIGLGAVAFVFVNITAITTALWVFLLLFALSGLPNITSQ
                     VGATTTAQRLCPPAALGRLQGLLSAATSAGAIVGSVAVGLLVDDIDVKALLNAQATLF
                     VLSGIVTYVGVIRRIGQQRPTGA"
     gene            1935..2969
                     /locus_tag="AC161X_02390"
     CDS             1935..2969
                     /locus_tag="AC161X_02390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988429.1"
                     /translation="MRKLCTFAYVMLDTPKTRKRVLGRFLLPILITAAALIAVGVANA
                     GRDTRADLEYLNTIQEQTVALSARGDALREVVSRLARIDRTELVTLVDSIRADLAVGR
                     EFAADDPPSGTLFAVNALYRQALDAWEAGIGGYASGLLAAADDPSSTVVVDNIANSLA
                     ELRAGDRLYVDLVAEMGRVDVPDSAAPMPTVVLMPANGELFSLSSAYAEAARSPNSGL
                     ALRPGLSVSQLVTDPLWEVDPTDVVVLPATDTVTFEVVLSNLGNVLSSPSTVRLVLTG
                     GEETVTLDEEFGAMQPDAQTSVRFEDVVVVPGGQYRVTATLVGLGPDIDLTDNEISVD
                     FIVNEGEGSG"
     gene            2994..>3579
                     /locus_tag="AC161X_02395"
     CDS             2994..>3579
                     /locus_tag="AC161X_02395"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014458.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA];
                     GO_process: GO:0016260 - L-selenocysteine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HIR4988430.1"
                     /translation="MIDARLLRESPDLIRESLRRRGSTIDLDRLTTLDSAVRAKRQEA
                     EGLRARQKEAGRLIATLDGPEKEAAIAEVAELANAVKEATAEADRLAAEFDQALLEVP
                     NLVAPDAADGHTDADSVEIKRVGNPRSDGLDHATLGERLDIIDTARGAKVSGSRFGYI
                     KGKGAMLEFSLVRWAMDHLVEAGFTPMLPPVLVRE"
BASE COUNT          666 a         1115 c         1176 g          622 t
ORIGIN      
        1 cgctggagcg cccgatcagg actcgattcg gtcccaggtc atatgaggct tcagccccga
       61 ccaggcggcc ccaaggggcg atcttgcccg gcaccaccga ggggacacag gtgaaatgac
      121 cgggcgacac gctgggagag gcctcgatga gcacggttgg tgggccctcg aggcgcagcc
      181 cggcctccgc tgcgtgctcg gacaaggcca gttcaagcct cttctgcagc ctggtcgggt
      241 tctcgtcgag gtctatggga ttcaccttca gcacgtactg gttggacgta gcgggaccgg
      301 tgatgtgctc gtaactggtg aggtctgcct cacgggcgat cctgcctgcc atctccgaag
      361 ggtggatccg tccggaaaag acgcgtccgg ccgcgccttc aaaaaggcgt tcgagacgac
      421 gttccaggct cctggccaga ttcacggatg agaggttacg gtcggtggtg gatagaggag
      481 gccgactacc gcaacaccag gcatggggtt acgatttcga agccctcgag ggcgagtggc
      541 ggaattggca gacgcgcagg attcaggttc ctgtgatcgc aaggtcgtgg gggttcaagt
      601 cccccctcgc ccacaccagc ccggttcagc gggaggctca ttaggcgccg gtcggccgtt
      661 gttggccgat acggcggatt actcccacgt aggtgacgat cccggagaga acgaaaaggg
      721 tcgcctgcgc gttgagtagt gctttgacat cgatgtcgtc gaccagcagc ccgacggcga
      781 ccgaacccac gatggcccca gcggaggtgg ccgctgagag cagaccttgc aggcgtccga
      841 gcgccgccgg cgggcagagt cgttgcgcgg tggtggtcgc cccgacctgt gaggtgatgt
      901 tgggcagccc gctcagggcg aatagcagga ggaacaccca gagtgccgtc gtgatcgctg
      961 tgatgttgac gaagacgaag gcgacagcgc cgagcccgat gtagccccac atcatcagtc
     1021 gagtcgggtc cgctcggttt cccagacggc caaccatcac cgcggcggcg atcccgccga
     1081 aagccgcgct cccgcggatg atcccaacag ttgcctcgtc gccgttgagc acgtcgacca
     1141 caaatgcgat gaacagcaca gggaacatcg cgaaggtcag agctgccacg gtttccgcgc
     1201 cgagatagcc tttgagcccg gcatggaggc ccagctctcg cagaccggcc ctcacacctg
     1261 cctcgtttcg atcggtcccg tcgcttgtgg tgaggctggc tgttggtgtt ctcaccaggg
     1321 tcgtagcgac ggccacggtg agaaacgtga tcgcatcgac cacgacaacc gtgctgagcc
     1381 cgcccagggc aaccgcgatt ccaccgagcg gagcacctag caaccgcgca atcgactgcc
     1441 ctgcgctgtt ggccgagttg gcggtcacca gctgctccga gggcacgatc ctgggcacga
     1501 ttgcaaacga agccgggttg ttgatctgcg ccagaagccc ctggatgccg gccaccaaga
     1561 acgccggcca cacgcggtct ggggtgacgg caagaagcgg gagcatgccg gcggcctgag
     1621 ccacgttggt ggcgatgatc gtgcgccgca gatcccaccg gtcagcgaga ctgccgccgt
     1681 atggcccgaa aacgacgccg atcaccagtt cggtgacaac gatcgtcgct gttgctcgcc
     1741 ctgagcctgt ctgggtgtag acaaacgccg gcaaagccac ggccaacagc cagtcaccta
     1801 cgttgttgac gaagccacca cccaggacca gcaggaagtc gcgattccgc cagaggctct
     1861 tgggcatcgg cggcatgttg tcacacatcg gccaggcgga acgctgactt ttccctgggc
     1921 gcagttaccg atcagtgaga aagctgtgta ctttcgccta cgtaatgctc gacacaccca
     1981 agacccgcaa gcgggtgctg ggccgattcc tgttgcccat cctcattact gcggctgccc
     2041 tgatcgcggt cggcgtggcc aatgccgggc gtgacacccg tgccgatctc gagtacttga
     2101 acacgattca ggagcagaca gttgccctct ccgcaagagg cgacgccttg cgcgaggtcg
     2161 tctcacgact ggcccggatc gaccgcaccg agttggtgac cctggtcgac agcatccgag
     2221 ccgacctagc tgtcgggcgg gaatttgcag cagacgaccc accctccgga accctgttcg
     2281 cggtcaacgc cctgtaccga caggctttgg acgcctggga agcagggatc ggtggctacg
     2341 cctcgggact tctagccgcc gccgatgacc catcgagcac cgtagtcgtg gacaacatcg
     2401 ccaactctct tgcggagctg cgagccggtg accggttgta tgtggatctg gtagcggaga
     2461 tgggccgggt ggacgttcca gactcagcgg caccgatgcc gactgtggtc ctgatgcctg
     2521 cgaatgggga gctgttcagc ctgtcgtcgg cctatgccga ggccgcccgc tcgccaaaca
     2581 gtggtttggc cctgcgacca gggctttctg tgagccagtt ggtgacggat ccactgtggg
     2641 aggtcgatcc aacggacgtt gtcgttctcc ccgccaccga cacggtgacg ttcgaggtgg
     2701 tgctctcgaa tctggggaac gtgctgagct caccgtcgac cgttcggctt gtgctcaccg
     2761 gtggggagga gaccgtcacg ctcgacgagg aattcggggc tatgcagcca gacgcccaaa
     2821 cctcggtcag attcgaggat gttgtggtgg tgcccggcgg gcagtaccgg gtaaccgcca
     2881 ccctggtggg gctgggcccc gacatcgacc tgactgacaa cgagatctca gtcgacttca
     2941 tcgtgaacga gggcgagggc tccgggtagc cagccggtac catccggcgc cccatgatcg
     3001 atgcccgact gcttcgagag agcccggatc taattcgcga gtcgcttcgg cgtcgcggct
     3061 cgaccatcga cctcgacaga ctgaccaccc tcgactccgc ggtccgagcc aagcgccagg
     3121 aggcggaagg cctccgggca cgccagaagg aggcggggcg actgatcgca acgctggatg
     3181 gcccggagaa agaggccgcc attgcggaag ttgccgaatt ggcgaatgcg gtgaaagaag
     3241 ccactgccga ggccgatcgt cttgccgccg aattcgatca ggcccttctc gaggttccca
     3301 atctggtggc gcccgacgct gccgatggtc acaccgatgc ggacagcgtg gagatcaagc
     3361 gcgtcggcaa ccctcggtcg gatgggctgg accatgccac gctcggtgaa cggctcgaca
     3421 tcatcgacac cgcgcgcggc gccaaggttt ctgggagcag gttcggatac atcaagggca
     3481 agggcgcaat gctcgagttc tccttggtgc ggtgggcaat ggatcacttg gtcgaggccg
     3541 gtttcacacc catgctccca ccagttctgg ttcgtgagg
//
LOCUS       DBNHXU010000090         6901 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_226842, whole genome shotgun sequence.
ACCESSION   DBNHXU010000090 DBNHXU010000000
VERSION     DBNHXU010000090.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6901)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6901)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6901
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_226842"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..569)
                     /locus_tag="AC161X_02400"
     CDS             complement(<1..569)
                     /locus_tag="AC161X_02400"
                     /inference="COORDINATES: protein motif:HMM:NF023495.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rnase Y domain-containing protein"
                     /protein_id="HIR4988431.1"
                     /translation="MDNPLAIGLSVLALVIGLGFGYLLARLAVTRRRERDGVSAEDLT
                     TSARLDAQRILAKAEEEGRAKAETYREREEANLEHRRLEATSLESRLVQREETLDQRA
                     SNLAQREKMLIDREEEVNESRGQVEHLKEEARFRLEQVSGIDVSAAKTEMLAQVEDEA
                     RREAMIVVRDLEIKAREEADRRARRILATA"
     gene            complement(770..2926)
                     /gene="recA"
                     /locus_tag="AC161X_02405"
     CDS             complement(770..2926)
                     /gene="recA"
                     /locus_tag="AC161X_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017976182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HIR4988432.1"
                     /translation="MGSEREKALDMALSQIERQFGKGAIMKMGEAANVKVDVISTGAL
                     SLDLALGIGGVPRGRIVELYGPESSGKTSLALHIVAEAQRNGGIAAFIDAEHALDPVY
                     AKAIGVDVDELLISQPDTGEQALEIADMLIRSGALDVLVIDSVAALVPRAEIEGEMGD
                     SHVGLQARLMSQAMRKLAGNLHKSKTTAVFINQLREKIGVMFGCFQYSTRVTLADGSR
                     EKIGKIVNQRLPVEVMAVDPVTGKVEPRKVINWFDNGNTDEFLQFTVYKPEGNGRAQF
                     AATANHLISTPGGWREAGELLAGDRVLMPLPHYLSEQQRQLVLGSLMGDGSISPKRPH
                     ADGPGIKSRFRFGHGPKQNAYAEWKAGLLAGVPLTISTHSKGGLMVETTPLVELDELR
                     ESVYVAGKKVFSWDYLKELTPFALAIWYLDDGSFAVRRKDGSAGRSDICVQAMDKGSQ
                     RRLVSLLNESYGLSCKLIERAGQAVVVFDRDGTEALHELISPYVPEAMSYKLLQHHRG
                     KGSVVVEPAEKTYELSAVPIIDIHVKPATRSMRRFDIEVEGHHNYLVDGVMVHNSPET
                     TPGGRALKFYSSVRLDIRRVETIKDGADSVGNRVKVKVAKNKMAPPFRLAEFDIMFGH
                     GISREGSLLDVAVDHGVVRKSGAWFTFDDDQLGQGRENSKRFLRENPEVAMQLQAKVY
                     ELVGLGEKSGESEGAEEVEDVIPSEAVPEDASSLEE"
     gene            complement(3034..3585)
                     /gene="thpR"
                     /locus_tag="AC161X_02410"
     CDS             complement(3034..3585)
                     /gene="thpR"
                     /locus_tag="AC161X_02410"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - RNA 2',3'-cyclic
                     3'-phosphodiesterase activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HIR4988433.1"
                     /translation="MVGSVGRVFAAVPMPAEVRLALDDRLRPLSLPGKVAPPENWHLT
                     LRFLGSVDEVTYERFISQLDPAELGRSFRMALGPMGAFPNSKRATVLWVGVSQGEGEL
                     GGLASICEEAAQAAGLPAEERPFRPHLTLSRIRPQADVGSLIESFGDAGIGWRCETMI
                     IYRSLLGSGGASYEPLEIFELKR"
     gene            complement(3570..4805)
                     /locus_tag="AC161X_02415"
     CDS             complement(3570..4805)
                     /locus_tag="AC161X_02415"
                     /inference="COORDINATES: protein motif:HMM:NF001813.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HIR4988434.1"
                     /translation="MIVEVIAVGTELLLGQIVNSNTSFMGAALAERGFDAHYQQTVGD
                     NIDRVAATIRLALSRADAVIITGGIGPTQDDLTREAVCTATGLEMVYSEAYAKELEEW
                     WSRRGREMPPSNLRQAYHPAGAELLPNPRGTAPGLALEHQGKWIFCVPGVPAEMEHLV
                     IAEVLPRLIGHSDDQQVIVSRVIRTWGRPESEVAEMLDDLYTGSTNPSIAFLASASEI
                     KVRLTAKADDVAEAESLIEPMEGEVRSRLGDSVFGTDDDTIEKILLRLLTELGYSIGT
                     AESMTGGMVATRLTDLPGSSAVMKGGLVAYDPELKQRLLGVSDTHEVVNVETAVAMAV
                     GARNLLGVDVAVAVTGSAGPDPLEKPAGTVIVAVATPDDVRGREIRFTGDRERVRTYG
                     TTAALHLTRLALIGQWWAP"
     gene            complement(4802..6172)
                     /gene="rimO"
                     /locus_tag="AC161X_02420"
     CDS             complement(4802..6172)
                     /gene="rimO"
                     /locus_tag="AC161X_02420"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HIR4988435.1"
                     /translation="MPGPNVWLSTLGCAKNQVDSEKLIAMLSEAGYGWADSPEEADVV
                     MVNTCAFIEEARAESVDVILELETRKRPGARSVVIGCMAQRFGSEVESALPEVDAVLG
                     IDRYGELVGVLDGLTGWHPVEMRRRPSMDILHATGRPVSDLPYAYLKVAEGCDKPCTF
                     CAIPSIRGKQRSRRPVEIREELALLVAGGVSEVVLVAQDLAAYGRDIQAPGGLPDLLA
                     FVSDVEGLRRIRLLYLHPREIRERLLLEITENEKVAPYFDLSLQHVAGPLLRRMKRPG
                     NYEKHLALIERIRELDPESALRSSFIVGFPGESDDDVELLADFLDEAMLDWAGFFPYS
                     AEPGTPAAEMGDQVPREKTMERLRYLSRIQDEMTEARNSEWLGRSDLVLVDQVEDGVP
                     VGRSHRQAPEIDGVVRLDRGEVGEWVKAEYVGVFGPDMEARFLPPVSEASVGGSTAAG
                     GDVGTR"
     gene            complement(6192..6446)
                     /locus_tag="AC161X_02425"
     CDS             complement(6192..6446)
                     /locus_tag="AC161X_02425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988436.1"
                     /translation="MDSKAQAFVEEAIRAVARGDVAGARTSVAQAYDTDHTLGAVADA
                     IYLACWQIEEDGLVTTSTWNTLADAVGSGHLLAVVEESRS"
     gene            complement(6446..>6901)
                     /locus_tag="AC161X_02430"
     CDS             complement(6446..>6901)
                     /locus_tag="AC161X_02430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988437.1"
                     /translation="ERFGTHHARDAHRLFETLGYDLNVYSSAVKLNTQYMHSIQFSLE
                     LDEEITVDQAKERLMANTRVAVTNKTSANQVFSFGRDHGYYGRIMSQTVVALDTLAVR
                     NRNEVVGFSFTPQDGNPLLSTVAAMLWYLDPESLDRRIDILRRYLFREI"
BASE COUNT         1284 a         2272 c         2038 g         1307 t
ORIGIN      
        1 gcggtggcga gaatcctcct cgcccgacgg tctgcctcct cgcgagcctt gatctccagg
       61 tcgcggacaa cgatcatcgc ctcgcgtcgg gcctcgtctt ccacctgggc cagcatctcg
      121 gtcttggcgg cgctgacgtc gatcccggag acctgctcca gacggaatct ggcctcctcc
      181 ttgagatgct cgacctgacc tcgggattcg ttgacctcct cctcacggtc aatgagcatc
      241 ttctcgcgct gtgccaggtt ggaggcgcgc tggtcgagag tctcctcgcg ttggaccagc
      301 ctggactcga ggctggtcgc ctccaatcga cggtgctcga gattggcctc ctcacgctcc
      361 cggtaggtct cggccttggc cctgccttcc tcctcggcct tggccaggat ccgctgggca
      421 tccagacgcg ctgaggtcgt caggtcttcg gccgataccc catctcgttc ccggcgccgg
      481 gtcacggcga gccgggccag caggtaccca aaaccgagac caatgacaag cgcaaggacg
      541 ctcagcccga tcgccagggg gttgtccatc atggtccttc ccccggggat ggaaacagac
      601 gtgccgagcc ctcacggact cggcacacga acggagctag cgacgaaagc gagtgaaatc
      661 acccgtcggc tcgatcgctc ctcctgatca cgtttctctc tccggtttgc agttcaaatt
      721 tcgtgtgctg agaccggatg gtacacaccg aagtcgcgaa taaaaacgtc tactcctcga
      781 gggacgatgc gtcctcaggg acagcctctg agggtatgac gtcctccact tcttccgctc
      841 cctcagattc cccggatttc tcaccgagcc caaccagttc gtagaccttg gcctggagtt
      901 gcatcgccac ttccggattt tccctgagga agcgcttgga gttctcccga ccttgcccca
      961 gctggtcgtc atcaaaggtg aaccaggcgc cgctcttgcg caccaccccg tgatctacgg
     1021 cgacgtcgag aaggctgcct tcacgggaga tcccatggcc aaacatgatg tcgaactcgg
     1081 cgagacggaa cggtggggcc atcttgttct tggccacctt gaccttcacc cggttcccga
     1141 cactgtccgc accgtccttg atggtctcca cccgacgaat gtcgagacgg accgatgagt
     1201 agaacttcag tgcccgacca cccggagtgg tttctggtga gttgtggacc atgacaccgt
     1261 cgaccaggta gttgtggtga ccctcgacct cgatgtcgaa gcggcgcatg ctcctggtgg
     1321 ctggcttgac gtgaatgtcg atgatgggca ccgccgagag ctcataagtc ttctcagccg
     1381 gctcgacgac aactgatccc ttgccacgat ggtgttgaag cagcttgtag gacattgctt
     1441 ccggaacgta cggggagatc agctcatgga gcgcctcggt cccgtcacgg tcgaagacga
     1501 caacggcttg gccggcccgt tctatcagct tgcagctgag cccataactc tcgttaagca
     1561 acgaaacgag tcgacgctgc gatcccttgt ccatggcctg gacacagatg tcggatcgac
     1621 ccgcggaacc atccttgcga cgaaccgcaa agctgccgtc gtccaagtac caaatggcga
     1681 gagcgaatgg ggtcagttcc tttagatagt cccagctgaa gacctttttc cccgctacgt
     1741 agacggactc tcgcaattca tcgagttcga ccaagggtgt ggtctccacc atcagcccgc
     1801 ccttggagtg agttgagatg gttaggggca cgccagcgag tagcccggcc ttccattccg
     1861 catatgcgtt ctgcttggga ccatgcccga aacggaacct cgacttgatc ccgggcccgt
     1921 cagcatgggg cctctttgga gagatggacc catcccccat caatgaccca agcaccagct
     1981 gtcgctgttg ctcggagaga tagtgcggaa gtggcatgag cacccggtca ccggcaagta
     2041 gctcaccagc ctctctccac cccccggggg ttgagatcag gtgattggca gtggccgcaa
     2101 actgtgccct gccattgcct tcaggcttgt agaccgtgaa ctgcaggaac tcgtcggtgt
     2161 tcccgttgtc gaaccagttg atgactttcc ttggctcgac ttttccagtc accggatcta
     2221 cggccatcac ctcgacggga agccgctggt tgacgatctt gccgatcttt tcacgagaac
     2281 cgtcggcaag agtcacccgt gtcgaatatt ggaagcaccc aaacatcaca ccgattttct
     2341 cccgtaactg gttgatgaaa acggcggtgg tcttcgactt gtgcaggttc ccggccagtt
     2401 tgcgcatggc ctgcgacatc aatctcgcct ggagcccgac atgggagtca cccatctcac
     2461 cttcgatctc ggcccggggc accagggcgg cgacagagtc gatcaccagg acatcgagag
     2521 ccccggagcg gatcagcatg tcggcgatct cgagcgcctg ctccccggtg tcgggttgcg
     2581 aaatcagaag ctcgtcgaca tcgaccccga tcgccttggc gtaaaccggg tcgagggcgt
     2641 gctcggcgtc gatgaaggcg gcgatccccc cgttgcgctg tgcctcggcc acgatatgga
     2701 gggcgagact ggtcttgccc gagctttccg ggccgtagag ctcgacgatg cggccccgtg
     2761 ggacaccgcc gattcccagc gccaggtcga gagagagggc accggtggag atgacatcca
     2821 ccttgacgtt ggccgcctct cccatcttca tgatggcgcc cttgccaaac tgccgctcga
     2881 tctggctgag cgccatgtcc agggccttct cacgttcact tcccacttcg gttccttcct
     2941 cgaagtccta tgacggtcaa cgtatctact gccaccgaca agaaagtctg gcttcgccgg
     3001 atactagacc gaacatgtgt tcgtggttgg taattacctt ttcaattcga agatttcaag
     3061 gggctcgtaa ctggcgcccc ccgagcccag cagggatcgg tagatgatca tcgtctcaca
     3121 ccgccacccg atcccagcgt caccaaacga ctcgatcagg ctccccacgt cggcctgggg
     3181 acggatccgg ctcagtgtga gatggggccg aaacggccgc tcctcagccg ggagaccggc
     3241 cgcttgcgct gcctcctcgc agatcgatgc cagtccgccc aactccccct cgccctgcga
     3301 caccccgacc cagaggacgg tcgctcgctt ggaattggga aaggcgccca tcgggcccag
     3361 agccatccga aagctccttc ccagctcggc tggatctagt tgggagatga agcgctcgta
     3421 ggtcacctcg tcgacgcttc ccaggaagcg aagggtgaga tgccagttct ctggcggtgc
     3481 caccttgccc ggcagtgaaa gaggtcggag cctgtcgtcg agggcgagtc ggacctccgc
     3541 cggcatcggg acggcggcaa acactctgcc tacggagccc accactgccc gatcaacgcc
     3601 agtcgggtca ggtgaagagc ggcggtggtc ccatacgtcc gcacccgctc ccgatcgccg
     3661 gtgaagcgga tctcccgacc ccgtacatcg tcgggggtgg caacggcaac gatcacggtg
     3721 cccgccggct tctcgagagg atcggggccg gcggagccgg tcaccgccac tgctacatcg
     3781 accccgagca ggttgcgggc cccgaccgcc atcgccaccg cggtctccac attcaccact
     3841 tcatgggtgt cgctgacccc gaggagccgc tgcttaagct cggggtcgta ggcaaccagg
     3901 ccgcccttca tcacggccga tgatcccggg aggtcggtga gacgggtcgc gaccatgcca
     3961 cccgtcatcg actcggcggt ccctatggag tagccgagct cggtgagcag ccggagcaag
     4021 atcttctcga tggtgtcgtc gtcggtcccg aagacactgt ccccgagacg ggaccggacc
     4081 tcgccctcca tcggctcgat cagggactca gcttcggcta catcgtccgc cttggcggtt
     4141 aatcgcacct tgatctcgga tgccgaggcg agaaaggcga tagaaggatt cgtcgacccg
     4201 gtgtagaggt catcgagcat ttcggcgacc tcagactcgg gacgacccca ggtcctgatc
     4261 accctgctca cgatcacctg ttgatcatcc gagtgaccta tgagccgggg caacacctcc
     4321 gctatcacca ggtgctccat ctcggcgggc acgccgggga cacagaagat ccacttcccc
     4381 tgatgttcca acgccagacc cggggcagtc ccccgtggat tgggcaggag ctcggcgccg
     4441 gcggggtgat aagcctggcg aaggttgctt ggcggcatct ctctcccccg ccgcgaccac
     4501 cactcctcga gctctttggc ataggcctcg ctgtagacca tctcgagacc cgtggcggtg
     4561 caaacggctt cgcgggtgag atcgtcttgg gtgggcccga tcccaccggt gatgatcacc
     4621 gcgtctgctc gactcaacgc gagacggatc gtggctgcga cgcgatcgat gttgtccccg
     4681 acggtctgtt ggtagtgggc gtcgaagcct cgctccgcca gcgcagcccc catgaaggag
     4741 gtgttggagt tgacgatctg accgaggagg agctcggtcc ccacggcgat gacctcaacg
     4801 atcaacgggt acccacatcc ccgcctgcgg cggtactccc cccaacgctc gcttcgctca
     4861 ctggggggag aaatctggcc tccatgtcgg ggccgaagac accgacgtat tcggccttca
     4921 cccactcccc gacctcaccc cgatcgaggc gaaccacccc gtcgatctcc ggtgcctgcc
     4981 ggtgtgacct ccccaccggc accccgtcct ccacttggtc gactaacacc agatcggacc
     5041 ggcccaacca ctccgagttc cgggcctcgg tcatctcatc ctggatccgg gagagatagc
     5101 gcaatcgttc catggtcttc tcccgcggca cctggtcgcc catctccgcc gccggagttc
     5161 ccggctccgc cgaatatggg aagaaccctg cccagtccag catcgcctca tccaggaagt
     5221 cggcgagcaa ctcgacgtcg tcatcacttt ctccggggaa tccaacaatg aacgaggagc
     5281 gcaacgcgga ctccggatcg agctcgcgga tccgttcgat cagagccaga tgcttctcgt
     5341 agttgcccgg ccgcttcatc cgacgcagga gcgggccggc cacgtgctgg agcgacaggt
     5401 cgaagtaagg agccaccttc tcgttctcgg tgatttccag gagcagccgc tctctgatct
     5461 cacgggggtg gaggtagagc agccgaatcc tgcgcagccc ctccacatcg gagacaaagg
     5521 cgaggagatc gggcaaaccg cccggtgcct ggatgtcccg cccgtaggca gcgaggtcct
     5581 gggccaccag gaccacctcg gagacaccac cagcaacgag cagggccaac tcctcgcgaa
     5641 tctcgaccgg tcgccgcgat cgttgcttgc cccggatcga ggggatggcg cagaaggtgc
     5701 acggtttgtc gcaaccctcg gccaccttca aataggcata ggggagatcg gagaccggtc
     5761 gcccggtggc gtggaggatg tccatgctcg gacgtcggcg catctcgacg ggatgccagc
     5821 ctgtgaggcc atcgaggacc ccgaccaatt cgccatatcg atcgataccg aggaccgcat
     5881 ccacctcggg caaggccgac tcgacctcag acccgaagcg ctgagccatg cacccgatca
     5941 ccaccgatcg agctcctgga cgctttctgg tctccagttc gaggatgacg tcgaccgact
     6001 cggcccgggc ctcttcgatg aaggcacagg tgttgaccat tacgacatcg gcctcttcag
     6061 ggctgtccgc ccagccgtaa ccagcctcgg agagcatggc gatcagcttc tccgagtcga
     6121 cctggttctt ggcacatccg agcgtgctca accaaacatt gggaccgggc atcgccttag
     6181 cctaagacga gtcaggaccg tgactcttca accactgcca acagatggcc cgaaccgacc
     6241 gcgtcggcaa gggtgttcca ggtcgaggtg gtcacgagcc cgtcctcctc gatctgccag
     6301 caggccaggt agatggcgtc ggcaaccgcc cccagggtgt gatcggtgtc ataagcctgt
     6361 gcaacggaag tccgggcccc ggccacgtcg cctcgagcca ccgctcggat cgcctcttcg
     6421 acgaaggcct gggccttcga gtccatcaga tctcgcggaa tagatagcgc cgcaagatgt
     6481 cgatgcgacg atcgagggat tccgggtcga ggtaccagag catggccgcc acggttgaga
     6541 gcagtgggtt cccgtcctga ggggtgaagg agaatccgac cacctcgttc cggttgcgca
     6601 cagcgagggt gtcgagggcg accaccgttt gactcatgat ccggccgtag tacccgtggt
     6661 cccgtccgaa ggagaacacc tgattggccg aagtcttgtt ggtgaccgcc acccgcgtgt
     6721 tggccatcaa ccgctccttc gcctggtcaa cggtgatctc ctcatccaac tccaaagaga
     6781 actgaatcga gtgcatgtac tgggtgttga gcttcactgc cgaggagtag acgttgaggt
     6841 catagcccaa ggtctcgaag aggcggtggg cgtcacgggc atggtgggtt ccgaaccgct
     6901 c
//
LOCUS       DBNHXU010000091         3521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2606268, whole genome shotgun sequence.
ACCESSION   DBNHXU010000091 DBNHXU010000000
VERSION     DBNHXU010000091.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3521
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2606268"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..374)
                     /locus_tag="AC161X_02435"
     CDS             complement(<1..374)
                     /locus_tag="AC161X_02435"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020521572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR4988438.1"
                     /translation="MSLEHDLDALAAAEEFSGVVRVDRAGETESAKAYGTADRGWAIP
                     NEINTRFGTASGSKGLTALAVVSLIEEGVLDLSTTARSLLGSDLPLISDEVTVEHLLS
                     HRSGIGDYLDEDAEYDLNDYMMP"
     gene            complement(392..700)
                     /locus_tag="AC161X_02440"
     CDS             complement(392..700)
                     /locus_tag="AC161X_02440"
                     /inference="COORDINATES: protein motif:HMM:NF017273.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HIR4988439.1"
                     /translation="MSDFALVLGVAAITFTTRVAFLLKPRSAPGGRVGKFLEVFPLAL
                     FIAIATNGLAAPAGTPELTANIAAAFGGVVGGIVFRRSLWGVIGVGAACFYLARALVG
                     "
     gene            complement(693..1361)
                     /locus_tag="AC161X_02445"
     CDS             complement(693..1361)
                     /locus_tag="AC161X_02445"
                     /inference="COORDINATES: protein motif:HMM:NF015549.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HIR4988440.1"
                     /translation="MFGNPSFRQGAREAFFLVFAVGAFALAYGVLAVDAGFSPALTLF
                     SSAIIVSGAAQFTMVGLVAAGPVPILLAATGLGLRHLPMSAKLADIIGPQPLRTRLRL
                     AFILVDETFGLTIRAYDAGVEDPVAYKTAADLMLYTGWIIGTAIGAWFGSSIDPGAVG
                     IGVLFGLLFMGLAAPMVNVRRDWVVAGASVVATLIAMKTLPVAWQISTAAFAASLVGL
                     VIDE"
     gene            complement(1374..2861)
                     /locus_tag="AC161X_02450"
     CDS             complement(1374..2861)
                     /locus_tag="AC161X_02450"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF013015.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HIR4988441.1"
                     /translation="MKVRTLCVWFPMWSLGRPDAPSSDPTLIVDDRVVGATSEVLAAG
                     VTLGMPRREAEALAPFATVLLRDPGEEARRFEPVVEVIEDLIPRVEVVSPGLVYVPVS
                     GAVRFYGGEAALADRVVKEVDNLVGHLPGGQALLGVADGPFAARWASATATVGQPQVV
                     EDTIGFLSRLDLATLRESVNGEELIDTFRWLGIGTLGELARLPRDALATRFGNPGALA
                     HRLASGEDRPVDPRAISPDLAVDAAFEDPLETLDAVAFASRALSERLLRGLRMNGVAP
                     HRVSILAESATGQTRERIWRSADPFTEKALADRVWWQLRAWVEAGGVPGGIVRLRIAP
                     ADLSDQGRQLGLLQNETSRIEAERALTRAQALLGPDAVLQGRAQGGRMPQERVAWSRW
                     GEPPTDAARDPDAPWPGATPGPSPALVPPRLQPLEVEWDEGMPSRVRLGTRWEPVLTW
                     SGPWRLSARWWLGEKDADRYQLVTSVGALLCVVAEGRAYLAGIYD"
     gene            complement(2791..3435)
                     /locus_tag="AC161X_02455"
     CDS             complement(2791..3435)
                     /locus_tag="AC161X_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988442.1"
                     /translation="MGIEHLVLEKPSRFFDMEGSLRLQPGRITEMVGVSGTGLTRLGL
                     RLLAEPSQVAPVVVLDTRSWISPEAAWETGVLAEHLVIVRCDDSRIWPKVAAALLEGV
                     HALYAEVPAQIKDQDLRRLAALARARGVSVAMRPMGNGLPSGVAHLRLRALGVTWEGA
                     DRGHGRLRRRRLVLEASGKGSAGVTRRFEVEDEGSDVVRVVSDVVVGEARRSVV"
BASE COUNT          706 a         1243 c         1065 g          507 t
ORIGIN      
        1 ggcatcatgt agtcgttgag gtcgtactcg gcgtcctcgt cgagatagtc accgatcccg
       61 gagcgatgag acaacaggtg ctcgacggtc acctcgtcgc tgatcagcgg aaggtcgctc
      121 ccgagcagtg accgggccgt ggtggaaagg tcgagaactc cctcttcgat gagactcacc
      181 accgcgaggg cggtcagacc cttcgaccca ctggcggtcc cgaaccgggt gttgatttca
      241 ttggggatcg cccaaccgcg gtccgccgtc ccgtatgcct tggccgactc ggtctctccg
      301 gcccggtcga cccgaaccac acctgagaac tcctctgcgg ccgccaatgc atcgaggtcg
      361 tgttcgaggg acatcgaccg gagggtatct ctcacccgac caaggctcga gccaaataga
      421 agcaggcggc accgaccccg atcactcccc aaagcgaacg acggaacacg atgccaccga
      481 ccaccccgcc aaaggccgca gcgatgttgg cagtgagctc gggggtcccc gccggagccg
      541 ccaggccatt ggtggcgatc gcaatgaaca gcgccaaagg aaacacctcg aggaacttgc
      601 ccactcttcc tcccggtgcc gagcggggct tcagaagaaa cgcgaccctc gtggtgaacg
      661 taatcgccgc aaccccgaga actagggcga aatcactcat cgatcaccag cccgaccagg
      721 gatgcagcaa aggccgccgt cgagatctgc catgccaccg gaagtgtctt catcgcgatc
      781 aacgtggcga ccacagacgc gccggccacc acccaatccc ggcgtacgtt gaccatcggc
      841 gccgccagcc ccataaagag gagcccgaag agcacaccga tcccgacagc gcccggatcg
      901 atcgacgagc caaaccaggc acctatcgcg gtgccaatga tccaaccggt gtacagcatc
      961 agatcggccg cggtcttgta ggcgaccggg tcttcgacgc cggcgtcgta ggccctgata
     1021 gtgagcccga aggtctcgtc gaccaggatg aaggcaagtc gcagccgggt cctgaggggt
     1081 tgtgggccga tgatgtcggc gagcttcgcc gacatcggaa gatggcggag cccgagcccg
     1141 gtggccgcca ggaggatggg aacgggaccg gcggcgacca gaccgaccat cgtgaactgg
     1201 gcggcgccgg agacgatgat ggccgatgag aacagggtga gggcgggaga gaacccggcg
     1261 tcgacggcga ggaccccgta ggccaacgca aacgcaccca ccgcgaaaac caggaagaag
     1321 gcctcacgcg ctccctggcg aaacgatgga ttgccaaaca tttgtcgacg ttatcaatca
     1381 taaatcccgg ccaggtacgc cctcccctcg gcaaccacac agagcagggc tcccacagag
     1441 gtcaccaact ggtagcggtc ggcgtccttc tcccccagcc accaccgggc gctgagacgc
     1501 cacggcccgc tccaggtgag gaccggctcc cagcgagtgc cgagccggac ccgggagggc
     1561 atcccctcat cccattccac ctccaaaggc tggagccgtg gtgggaccag ggcagggctt
     1621 ggcccggggg tcgctcccgg ccatggtgca tctggatccc gggcggcatc ggtcggcggc
     1681 tcaccccaac gggaccatgc gaccctttcc tggggcatcc tccccccctg ggccctcccc
     1741 tggagcacag catcagggcc gagcaacgct tgtgccctgg tcagagccct ttcggcttcg
     1801 atcctcgaag tctcgttctg aagcaaccca agctgtcgac cctgatcgga gagatcggcc
     1861 ggtgcaatcc gcaatctcac gatccccccg ggcaccccgc ccgcctcaac ccaggcccgg
     1921 agttgccacc acacccgatc ggcgagcgcc ttctctgtga aggggtccgc cgatcgccat
     1981 atccgctccc gggtctgacc ggtcgcggac tcggcgagga tcgatacccg atgcggagcc
     2041 accccgttca ttcgcaaccc gcgcagcaac cgctccgaca gcgcacgaga tgcgaaggcg
     2101 acagcatcca gcgtctccaa ggggtcctcg aaggcggcgt cgacagcgag atcgggtgag
     2161 atcgcccggg gatcgaccgg ccggtcctcc ccactggcca gccgatgggc gagagctccc
     2221 gggttgccga agcgggtggc cagggcatca cggggcaatc gggccagctc accgagggtg
     2281 ccgatcccca accagcggaa agtgtcgatc agctcctcgc cattgacgct ctcccggagc
     2341 gttgccagat cgagacgtga gagaaacccg atcgtgtcct cgaccacctg tggctggcca
     2401 actgttgcgg tcgccgaagc ccagcgggca gcgaacggcc catcggccac tccgagcaga
     2461 gcctggcctc ctgggagatg gccgacgagg ttgtcgacct ctttgaccac ccgatcggcc
     2521 agggctgcct ccccgccata gaagcgcact gcaccggaga cagggacata gaccagaccc
     2581 ggggaaacca cttcgacccg tgggatgaga tcctcgatga cctccaccac cggctcgaaa
     2641 cgacgggcct cctccccggg gtcgcggagc agcaccgtcg caaacggagc caaagcctcg
     2701 gcttcccgac gtggcatccc caaagtcact ccggcggcga gcacctcgct cgtggcgccg
     2761 acgacccggt cgtcgacgat cagggtcggg tcagacgacg gagcgtctgg cctccccaac
     2821 gaccacatcg gaaaccacac gcacaacgtc cgaaccttca tcctccacct cgaaacgccg
     2881 ggtcaccccg gccgatcctt ttccggacgc ctccagcacc aggcgtcgtc tcctcaatct
     2941 cccgtgtccc cgatccgccc cctcccaggt gacccccaat gcccggagcc ggagatgagc
     3001 caccccgctg gggaggccat tgcccatcgg ccgcatcgcc accgacaccc ctctcgccct
     3061 ggccagtgcg gcgaggcggc gcagatcctg atccttgatc tgggccggaa cctcggcata
     3121 gagggcatgt actccttcga gaagggcagc agcaaccttg ggccagatcc gcgagtcatc
     3181 gcaacgcacg atgaccaggt gctcggcgag gaccccggtc tcccaggcag cctccggcga
     3241 gatccagctt ctggtgtcga ggaccaccac cggggcaacc tgcgagggct cggcgagaag
     3301 tctcagaccg agacgggtga gcccggtccc ggagacaccg accatctcgg tgatccgacc
     3361 cggctggaga cggagcgatc cctccatgtc gaagaaacgg gaaggtttct ccaagacgag
     3421 atgctcgatg cccaaactga ctccgctatc gggcgctcaa tcatatcgaa catatgttcg
     3481 atagcaagag ggtctcaacc ctcttccagc tcctccaacg t
//
LOCUS       DBNHXU010000092         2708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_820967, whole genome shotgun sequence.
ACCESSION   DBNHXU010000092 DBNHXU010000000
VERSION     DBNHXU010000092.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2708
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_820967"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..231
                     /locus_tag="AC161X_02460"
     CDS             <1..231
                     /locus_tag="AC161X_02460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="dolichol-phosphate mannosyltransferase"
                     /protein_id="HIR4988443.1"
                     /translation="RATALRALPYRETEASGYGFQVEMALKAHQQGLAIVEVPISFRD
                     RTRGTSKMGTGIVLEAMKLVTIWGFQRLRFWK"
     gene            231..710
                     /locus_tag="AC161X_02465"
     CDS             231..710
                     /locus_tag="AC161X_02465"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988444.1"
                     /translation="MKVSARTAETSDLPTLERLYRGLETEMTELHLMWPLADGLAEPV
                     VESLNEALHDADTLVVVGEIEDYPFGFLVARVEGLLPQARGEKIGSIRLVFVEPEARE
                     VSVGESMRDYALEWLRAQGISKFDAHVLPGHRLVKNFFEAGGFSARSIVMHHDDNRE"
     gene            703..1104
                     /locus_tag="AC161X_02470"
     CDS             703..1104
                     /locus_tag="AC161X_02470"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR4988445.1"
                     /translation="MSKAPVVGVGVVLIDDNRLLLIRRGHEPSMGLWAVPGGKVNPGE
                     SLRDAAAREVKEETGLDVETGEVVWVGEHISEHDHIVIIDFEGQVVGGELSAADDATQ
                     ARWVGLDQVADYDLTPTMYDLVRLIRKSAPH"
     gene            1114..2442
                     /locus_tag="AC161X_02475"
     CDS             1114..2442
                     /locus_tag="AC161X_02475"
                     /inference="COORDINATES: protein motif:HMM:NF013696.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR4988446.1"
                     /translation="MSHDAVDLLQQLIRNQCVNDGTPESGFEFRSVETLAEFFGVRGE
                     VFEPAPGRQSVVYRVPGTDPEAPSLALLPHLDVVIVEKGGWSVDPFAAEIVDGFVFGR
                     GAVDMLNVAAAMAVAVRPYLTGEKRPRGDLVFCAVADEENGGRLGALPLVQDHWDLVG
                     ADFLLTEVAYPALSADGHTAVPVSVAEKGAYWSILETSGSPGHGSLPYGTDNALEKMV
                     TALAGVVETPAPAEITDGWISFVSNLGLDEESTRRLTDIDQVDDEIDRIAITDPTLAR
                     YIHAATHLTISSNILRSGTKTNVVADRAHAEVDIRGLPGMDRDFVDAHLRKAMGSAAG
                     HVEIRPMMDGDATISPIGNPLWEAIGDAVEELDGHRNLAPTLMTVATDARFWRAKGTV
                     CYGVGLFDDRMSFSELLNLFHGHDERVTVDSVIRTTDLYVRVLDRFFARA"
     gene            2439..>2708
                     /locus_tag="AC161X_02480"
     CDS             2439..>2708
                     /locus_tag="AC161X_02480"
                     /inference="COORDINATES: protein motif:HMM:NF024740.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR4988447.1"
                     /translation="MNVICDIDGVIYLDDTLVPGSDLALQRLHDAGVTVHFATNNSTK
                     APSTVSDKITRITGVEIDPGSIVTSSQAAVHLLGEDRGPVMVLGGD"
BASE COUNT          485 a          797 c          907 g          519 t
ORIGIN      
        1 cgcgcgacag cgctgcgggc cctcccttat cgggagaccg aagcgtcggg atacgggttc
       61 caggtcgaga tggctttgaa ggcccatcag cagggattgg ccatcgtcga ggtgccgatc
      121 agcttccggg atcggaccag agggacctcg aagatgggaa ccgggatcgt cctggaagcg
      181 atgaagctcg tgacgatctg gggttttcag cggttacggt tctggaagta atgaaagtct
      241 cggcgcgaac tgccgaaacc agcgacctcc cgactctgga gaggctctat cggggtctcg
      301 agaccgagat gaccgaactc cacctcatgt ggccgctggc cgatggtttg gccgaacctg
      361 tggttgagtc cctcaacgag gcgctgcacg atgccgacac gctggtggtc gttggcgaga
      421 tcgaggatta tccctttgga ttcctggtag ccagggtcga agggctcctg ccccaggccc
      481 ggggcgagaa gatcggctcg atacgcctgg tctttgtcga accggaggca cgagaggttt
      541 cagtggggga gtcgatgagg gactacgccc tcgaatggct gcgggcccag ggcatctcga
      601 agttcgacgc ccacgtcttg cccgggcaca ggttggtcaa gaacttcttc gaggcaggtg
      661 ggttcagcgc gcgatccatc gtcatgcacc acgatgacaa ccgtgagtaa ggcacctgtc
      721 gtcggcgtgg gcgtggtctt gatcgacgac aaccggttgc tgctgatccg gcgaggtcac
      781 gagcccagca tggggctgtg ggctgtcccc ggtggcaagg tgaacccggg ggagtcgctc
      841 cgtgatgccg cagcccgcga ggtgaaagag gaaaccgggc tcgacgtcga aaccggcgaa
      901 gtcgtgtggg tcggcgagca catctccgag cacgaccaca ttgtgatcat tgacttcgaa
      961 ggccaggttg tcggtgggga gctctcggcc gcggacgacg caacacaggc tcgttgggtc
     1021 gggctggacc aggtggccga ctacgacctg acgccgacga tgtatgacct ggtgcggctc
     1081 atccgcaagt cggcccctca ttaggttgac ccggtgagcc acgacgcggt tgacctgctg
     1141 caacagctga tccggaatca gtgtgtcaac gacggcactc cggaaagcgg cttcgagttt
     1201 cgctcggtcg agaccctcgc cgagttcttc ggagttcgtg gggaggtgtt cgagccggcg
     1261 ccgggacgcc agtcagtggt gtatcgggtg cccggcaccg atcccgaggc gccgtcgttg
     1321 gccttgctcc ctcatctcga cgtcgtgatt gtcgagaagg gcggctggtc ggtcgaccca
     1381 tttgcagccg agatcgtcga cggtttcgtc ttcggcagag gtgcggtgga catgctcaac
     1441 gtggcggcgg cgatggcggt ggcggtccgt ccctacctga ccggagaaaa gcggccccgt
     1501 ggcgacctgg tgttctgtgc tgtggccgac gaagagaacg gtggacggct cggggcgttg
     1561 ccgttggtcc aggatcactg ggatctggtt ggagccgatt ttctattgac tgaagtggcc
     1621 tatcccgcct tgtcggcgga cgggcacacg gcggtcccgg tgtccgtggc tgagaagggc
     1681 gcctactggt cgatcctgga gacatccggc tcgcccggac atgggtcgct tccgtacgga
     1741 accgacaacg cgctggagaa gatggtcacc gctctcgccg gggtagtcga gacacctgcg
     1801 cccgccgaga tcacagatgg ctggatttcc tttgtgagca acctcgggct cgacgaggag
     1861 tcgacccggc gcctgaccga catcgaccag gtcgacgacg agatcgaccg catcgccatc
     1921 accgacccca ctttggcgcg ctacatccat gccgccaccc atctcacgat ctcctcgaac
     1981 atcctccgtt cggggacgaa gaccaacgtc gtggccgatc gggctcacgc ggaggtcgac
     2041 atcagagggc tccctggcat ggaccgtgat ttcgtcgacg cccacttgcg aaaggcgatg
     2101 ggctccgccg ctgggcatgt cgagatccgt ccgatgatgg acggcgacgc cacgatctcc
     2161 ccgatcggca acccgttgtg ggaggccatt ggcgatgcgg tcgaagagct ggacgggcac
     2221 cgcaatctgg cacctacgct gatgacggtg gccacagacg cccgtttttg gcgggcgaaa
     2281 ggcacggttt gctacggagt gggcctgttc gacgatcgga tgagcttctc tgagttgctg
     2341 aacctgtttc acggccacga tgagcgggtc acggtcgact cggtgattcg caccacggat
     2401 ctctacgtga gagttctcga ccgcttcttt gcgagagcat gaacgtcatc tgtgacatcg
     2461 acggtgtcat ctacctcgat gacacccttg tgccgggatc ggatctggcc ctgcaacgac
     2521 ttcacgatgc gggtgtaact gtccatttcg ccaccaacaa cagcaccaag gcgccgtcga
     2581 ccgtcagcga caagatcacc cggatcacgg gcgttgagat cgatcccggt tcgatcgtca
     2641 cctcctcaca ggcggcagtc catctcctcg gtgaggatcg gggcccggtc atggtgctcg
     2701 gaggtgat
//
LOCUS       DBNHXU010000093        11091 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4789654, whole genome shotgun sequence.
ACCESSION   DBNHXU010000093 DBNHXU010000000
VERSION     DBNHXU010000093.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 11091)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11091)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11091
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4789654"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1633
                     /locus_tag="AC161X_02485"
     CDS             <1..1633
                     /locus_tag="AC161X_02485"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018656156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HIR4988448.1"
                     /translation="IRQLAAGSYTILPLGMRVRAKVAGIIREEMNAIGGQEILPPTMH
                     PAEIWRTSGRYELMGDNLFKLVDRKEADLVLGMTEEEIFAITASELFSYRDLPQIWYQ
                     MHTKFRDEPRPKSGLLRVREFTMKDSYTLDIDRAGLDVGFEKHREAYFRIFRRWGLDP
                     VDVEASSGAMGGKESVEFMVRSDAGEDWIITCSNCGYRANVERALSAVAPVDDGVGAE
                     LERFPTPGLRTIKALAGAHPDVAPPDRQIKTLVYVLDGEPVLVLLRGDHDLQEQKLQD
                     ATGAIDIRPAEPEEIRPLLGADPGSLGAVGVKGPTVIADNALAGRKSLVTGANEDDWH
                     YSGVDVGRDIGVGVWAELRAVDAGEPCSVCANPLQRWKGIEVGHIFKLGTKFSEAFGA
                     YVQDENGVSHPIIMGSYGIGVERGMAAVVESSYDDGGIIWPVSVAPYEVVITVVRPDD
                     GPTMEAANRLYDELTSSGIEVLIDDREERPGVKFADSELIGIPYRVTVGPRGVADGSV
                     ELTARRGMVKSEVGLADLVSTVKESVESERFGAQL"
     gene            1718..2197
                     /locus_tag="AC161X_02490"
     CDS             1718..2197
                     /locus_tag="AC161X_02490"
                     /inference="COORDINATES: protein motif:HMM:NF014617.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HIR4988449.1"
                     /translation="MTQLTTVADIWPVVEDYLAAEHLELDDVELSGRGPATVLRIVVD
                     GKDVDIDRLAEVSRGVSRLLDNSTELDDPYQLEVTSPGLERKLRRPSHYAKSVGREVV
                     AKVAVEGGKTTVRGVIADAGERSFTVEAEGESQVVNYEDVLTANTVFRWEKAPKPGH"
     gene            2207..3361
                     /gene="nusA"
                     /locus_tag="AC161X_02495"
     CDS             2207..3361
                     /gene="nusA"
                     /locus_tag="AC161X_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009708772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HIR4988450.1"
                     /translation="MKLDTMMEAIEVLARERGVAVDTILDAFANALVAAYKRSPGAAE
                     EARVTIDPDSGEVTVYAQELDEEGNVTAEWEDTPEGEAFGRIAAQSFKQVMQHKLREA
                     KREHVYEMYEGREGDLVTGLVQQVDYRSVILDLGDAEAILPGSERIPFERLERGNRVK
                     ALILEVRNEAKGAQIVVSRSHPDLVRRLLELEVPELTDGTVEIVNIAREPGHRTKIAV
                     VSHDSNVDPKGACVGARGSRVRQVVNELRGEKVDVVQWRDDIGQFIAEALGPAKVKEV
                     HINEEEKTADVVVSEHQLSLAIGKEGQNARLAARLSGFKVDIRSDNEGVEGEETPAAA
                     ADEPAVEEPVLEEAVPEASDEDAVEPELEVSPETSDDEDAVEASEDDESE"
     gene            3358..5352
                     /gene="infB"
                     /locus_tag="AC161X_02500"
     CDS             3358..5352
                     /gene="infB"
                     /locus_tag="AC161X_02500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HIR4988451.1"
                     /translation="MRVYELARDLGLDSKVVLEQAQTLGLDVKTASSGLDDESAELLR
                     LAIAGDEPEAALVVETLTPEAEPEPEDEAEAEDEAQVGLASLKEGASVAEFAESLGVP
                     SSEIVKILLLRGMPVGAGQPMPTDLMEEIAESYGFIVDVEETAPPPAVAERPEHEDKA
                     DELKPRPPVVTVMGHVDHGKTTLLDRIRKTSVVDAELGGITQHIGAYQVEVNGHLVTF
                     IDTPGHEAFTALRARGANITDIVILVVAANDGVMPQTVEAISHAKAAGVKIVVAVNKI
                     DVPGADPTRVRTELTEHNIITEELGGDVPSVEVSALNGDGVEDLLEVVDLIAQLEEYK
                     ANPDAPASGVVVEARYETGKGAVATVIIQRGTLNKGDSFVAGAVAGRARGLMDENGNQ
                     IKSAGPSDPIQIMGWDDVPVAGDYFEAVESDREARRLATEREQELKTTSQAVPSARDR
                     LQSLLDQLRTDEAHLNLIVKADAQGSLEALRESIGKIEREGGKIQIVHGAVGGINEND
                     VTLAEVTESVIVGFNVRPEPKARKAAEQAGIEIRTYGIIYEMLDDVELLLVGQLAPVE
                     HEQVLGTAEVRAIFKVPRAGTVAGCYVVEGVIQRGAKARLLRDGIVIHDGQISTLKRF
                     KDDVREVAAGFECGMSFENYNDVKEGDSIEAYLIREVART"
     gene            5352..5645
                     /locus_tag="AC161X_02505"
     CDS             5352..5645
                     /locus_tag="AC161X_02505"
                     /inference="COORDINATES: protein motif:HMM:NF016351.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF503 domain-containing protein"
                     /protein_id="HIR4988452.1"
                     /translation="MRAAALRVELHLPGPQSLKAKRAILRPVTEGIKRLGSFSVAEVD
                     HHDSWQRAAIGVAIVARDGESLAMQIAKLRRYLDSRLEVEVLDIQMTELEDPE"
     gene            5642..6001
                     /gene="rbfA"
                     /locus_tag="AC161X_02510"
     CDS             5642..6001
                     /gene="rbfA"
                     /locus_tag="AC161X_02510"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HIR4988453.1"
                     /translation="MRETSPRMLKVNSTLREVLAEELERMNDDRLEMVSITSVETSPN
                     LRSATVFVDVLGAEAQEAALVALGRASPRLQKAIGRQVRMKFTPQLEFAIDPGVLGGE
                     RIETILRNLQQDGDGAE"
     gene            5998..6969
                     /locus_tag="AC161X_02515"
     CDS             5998..6969
                     /locus_tag="AC161X_02515"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013528.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HIR4988454.1"
                     /translation="MIDRAILERAAAAIASADSLALACHIGPDGDALGSMLGFGIAAE
                     NAGKKVIASFGSPFVVSASLAFLPTRLLVPPQAFPADPPMMVVFDAGSQDRLGELAEN
                     ASQAAITIVIDHHITNEGFGDIAVVDGEAAATGELVAHLLQILGWPLTPEIATCLHTA
                     LVTDTGRFQYGNTTPSTLMLAAGLVAAGADPPEISRHVYEEAPFGYLKVAGAALGRAE
                     LDEGKGVVTAVVTQADLSEAGIDWGDIDNLINTVRLAVEADVAVLAKVHADGKVKLSL
                     RSRGATDVGSLAVAMGGGGHRLAAGITYVGDVEDALNEVRQRVEDYR"
     gene            6966..7841
                     /gene="truB"
                     /locus_tag="AC161X_02520"
     CDS             6966..7841
                     /gene="truB"
                     /locus_tag="AC161X_02520"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HIR4988455.1"
                     /translation="MTKGFLVVDKPGGITSNAVVGRVRKATGIKKVGHAGTLDPMATG
                     AMVVAIGPVTRLIRFVQEQPKEYLATAQFGVATDTLDADGAVLEREPMDFSEQELVEA
                     AERFVGSILQIPPMVSALKHQGQRLYELARRGEVVEREARPVEVYELEILSVGPGPYP
                     DVEFRVVCGKGTYVRSLAADMASVLGGSAHLTALRRTRIGSLEVDVVGVAVDELEGWE
                     ESLLTPSQALCDLPAVTVGDDTAQGVSNGLRFLGGELVDAPDGVVRVLDGEGELLAIY
                     RSSGAQAVPEVVLAS"
     gene            7838..8764
                     /locus_tag="AC161X_02525"
     CDS             7838..8764
                     /locus_tag="AC161X_02525"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006593571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HIR4988456.1"
                     /translation="MRVLTSPWASWHAETDPTAVTVGVFDGVHLGHRTLIDRLRATGL
                     VPTVLTFDPHPAEVLSPGTHPRLITSIDERLALLEGAGVELVGVLDLGEIRHLHPGEF
                     VTQVLEERLAMAAMVMGTDFHFGKDRSGDVAFLRSAGDLNGFKVEVVELQTVDGIVSS
                     SGIRALLELGEVRKAEVLLGSRYLMSNVVVHGDKRGREIGFPTANLEPPDRKVIPGIG
                     VYAAFASLEGQRHQAAVNVGVRPTFGGGELVVEAYLLDFNDDCYGETVTLEFVERLRP
                     ELKFAGVPELVEQMTDDVVKVRHILSVEPKGA"
     gene            8757..9902
                     /gene="proB"
                     /locus_tag="AC161X_02530"
     CDS             8757..9902
                     /gene="proB"
                     /locus_tag="AC161X_02530"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR01027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HIR4988457.1"
                     /translation="MPDTGRHRIPVGMPVVMKAGSSSLVQRSGELDAEALASTADLVA
                     GMWELGYPTLLVSSGAVAAGLPALGLDRRPKDVPGLQVAAAVGQGRLMERYSALFAER
                     GRVVGQVLLTKDLLANRSQYLHAREALERMLSLGVVPIVNENDTVVVDGLRLGDNDRL
                     AAIVSHLVSAGLLVILTDTGGIYSADPRTVADAELLSAVRPTDAALDVVVSGEPGPFG
                     SGGAATKVAAARMAAWSGIPTVIADAREGDVLARAAAGEEIGTWVTPHETKLPARKLW
                     IAFGQPTEGKVVVDPGATKALLEGGKSLLAVGVLSVDGAFDAEGAVEVFDGDGRLVGK
                     GLTTMSADQARQSVGRHSSEVGGVVIHRDDFVLLAVEQSAPVDRLAP"
     gene            9899..>11091
                     /locus_tag="AC161X_02535"
     CDS             9899..>11091
                     /locus_tag="AC161X_02535"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0055129 - L-proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HIR4988458.1"
                     /translation="MIDVIGKRARDASRELAQSPGEVREHALRQMAEALEKATSALLV
                     ANEADLEQAAGEGIGGALLDRLRLTEARVRGMASGLQTVAALPDPVGRETGGWVLHNG
                     IRLHRVRAPLGVVAVIYEARPNVTADAAGLCIKSGNAVILRGSSYALRSNMAISNVLR
                     EALSNIGLPGDAVQLLEDTSRDGAKALMQATEWVDLLIPRGGPGLIDAVRADATVPTV
                     IDGAGNCHVYVDAAADLDMARSIVVNAKVQRPGVCNAAETLLVHEAVADQFLSDVAKA
                     LVAEGVELRGDQRSRELVPEMLEASDDDWATEFLDLIMAVKVVPDVDAAIDHIRRFGT
                     GHTEAIVTEDRSAADRFVGGIDTAVVAVNVSTRFTDGEEFGFGAEIGNSTQKLHVRGP
                     MGLESL"
BASE COUNT         2026 a         3290 c         3786 g         1989 t
ORIGIN      
        1 cattcgccag ttggccgctg ggtcttacac catcctcccc ctcgggatgc gggttcgggc
       61 caaggtcgcc gggatcattc gggaggagat gaacgcgatc gggggccagg aaatcctccc
      121 tccgaccatg catccggccg agatctggcg cacttcgggg cgctacgagt tgatgggcga
      181 caacctattc aaactcgtcg atcgcaaaga ggccgacctg gtcctgggca tgaccgagga
      241 ggagatcttc gcaatcaccg cctcggagct cttttcatac cgtgacctgc cccagatctg
      301 gtaccagatg cacaccaagt ttcgggatga gcctcggccg aagtcgggtc tgctccgggt
      361 tcgtgagttc acgatgaaag actcctacac cctggacatc gaccgggccg gtctggacgt
      421 gggtttcgag aagcaccgcg aggcgtactt ccggattttc cgacgctggg gcttggatcc
      481 tgtcgacgtc gaagcatcct ccggcgccat gggcgggaaa gagtcggtcg agttcatggt
      541 gcgcagcgac gccggcgagg attggatcat cacctgttcc aactgcggct accgggccaa
      601 tgtggaacgg gctctctccg ccgtggcccc cgtggacgat ggggttgggg ccgagctcga
      661 gcgattccca acacccggcc tgaggacgat caaggccctc gcaggagccc accccgatgt
      721 ggctccgccg gaccgtcaaa tcaagactct cgtctatgtc ctcgacggtg agccggttct
      781 cgtcctgctc cgtggggacc acgatcttca ggagcaaaag ctgcaggatg cgaccggagc
      841 catcgatatc cgacccgccg agcccgaaga gattcgtccg ttgctcggcg ccgaccccgg
      901 gagcctggga gcagtcggcg tcaaggggcc gaccgtgatc gctgacaatg cgttggccgg
      961 tcgcaagagc ctggtgacgg gggccaacga agacgattgg cactattcag gagtcgacgt
     1021 cgggcgtgac atcggtgttg gggtatgggc cgagttgcgg gccgtcgacg ccggcgaacc
     1081 gtgctcggtt tgtgccaacc cgctgcagcg ctggaagggg atcgaggtcg ggcacatttt
     1141 caagctggga accaagttct ccgaagcatt cggggcctat gtgcaggatg agaacggggt
     1201 cagccacccg atcatcatgg gctcctatgg gatcggcgtt gagcggggaa tggccgccgt
     1261 tgttgagtct tcctatgacg acgggggaat catctggccg gtctcggtcg ccccgtatga
     1321 ggttgtgatc accgtggtgc gccccgatga tgggccgacg atggaggccg ccaaccggct
     1381 ctacgacgag ctcacctcct cgggcatcga ggtgctgatt gacgaccgcg aggagcgtcc
     1441 gggggtgaaa ttcgccgact cggagctgat cgggatcccg taccgggtga cggtcggccc
     1501 tcgaggtgtc gctgacggct cggtggagct gactgcgcgg cgtggaatgg tgaaatccga
     1561 ggtcgggctc gccgatctgg tgagcactgt gaaggagtca gtcgagtctg agcggttcgg
     1621 cgcccagctc tgacaactca cttataatca gatctggaca acaggacgct ttttggatca
     1681 ggtgggcgcc tttggtaccc acctgacttg ttgtggagtg acacagttga cgacagttgc
     1741 tgacatctgg ccggtggtcg aggattatct cgccgccgag catttggagc tcgacgatgt
     1801 cgagttgtcg gggcgcgggc cggccacggt gctgcgcatc gtcgtcgacg gcaaagatgt
     1861 cgatatcgat cggctcgccg aggtttctcg gggtgtttcc cgtctgctgg acaattcgac
     1921 ggagctcgac gacccgtatc aactcgaagt cacctcaccg ggtttggaga gaaaactccg
     1981 tcgacccagc cactacgcaa agtccgttgg ccgtgaggtc gtcgccaagg tcgctgtgga
     2041 aggcggcaag acgaccgtca ggggtgtcat tgccgacgca ggcgagcgct ctttcacagt
     2101 cgaggccgag ggcgagagcc aggttgtcaa ttacgaagac gtgctcaccg cgaacaccgt
     2161 gttcaggtgg gaaaaggccc ccaagccggg ccactgaagg agaatcatga agctcgacac
     2221 gatgatggag gcgatcgaag tcctcgcccg ggagaggggc gtggcggtcg acaccatcct
     2281 cgacgccttt gccaacgccc tggttgccgc ttacaagcgg agcccgggcg cggccgaaga
     2341 ggcccgggtc accattgacc ccgactccgg cgaggtgacg gtctatgccc aggaactgga
     2401 tgaagagggc aatgtcaccg ccgaatggga ggacacgccc gagggcgagg ccttcgggag
     2461 gatcgcggca cagtctttca agcaggtcat gcagcacaag ctgcgggaag ccaagcgcga
     2521 gcacgtgtac gagatgtacg agggccggga aggcgatctg gtcaccgggc tggtgcagca
     2581 ggtcgattac cgatcggtca tcctcgacct cggagacgct gaggcaatcc ttcccggatc
     2641 ggagcgcatc ccttttgaac gcctggaacg gggtaatcgg gtcaaggcgc tgattctcga
     2701 ggttcgcaac gaggccaaag gagctcagat cgttgtttcc cggagccatc ccgacctggt
     2761 gcgccgactc ctcgagctcg aggtccccga actgacagac ggcacggtcg aaatcgtgaa
     2821 catcgccagg gagccaggtc accgcacgaa gatcgccgtt gtctcccacg attccaacgt
     2881 ggatccgaag ggcgcctgtg tcggtgcccg gggatcgcgg gttcgtcagg tggtcaacga
     2941 gctgaggggt gagaaagtcg atgtcgtgca atggcgcgat gacatcgggc agttcattgc
     3001 cgaggcactc ggcccggcca aggtcaaaga agtccacatc aatgaggagg agaagaccgc
     3061 cgacgtggtc gtctccgagc atcagctctc gctggctatc ggcaaagagg gccaaaacgc
     3121 gcggttggcg gcccgtctct ccggattcaa ggtcgacatt cggtcggaca acgaaggagt
     3181 agagggcgaa gagacaccgg cggccgctgc agatgaaccg gctgtcgagg aaccggtgct
     3241 cgaggaagcc gtgcccgagg cgtctgacga ggatgcggtc gagcctgaat tggaggtctc
     3301 ccccgagacg agtgacgacg aggacgccgt cgaggcgtcg gaggacgacg agagcgaatg
     3361 agggtctacg aactagcccg ggatctgggc ctcgactcga aggttgtgct cgagcaagca
     3421 cagacgctcg ggctcgatgt gaaaacggct tcttcaggtc tcgacgacga gtccgccgaa
     3481 ctgcttcggc tggcgatcgc cggtgacgag cctgaggcgg ctttggtagt tgagaccctt
     3541 accccagaag cggagccgga gcccgaggac gaggccgagg ctgaggacga ggctcaggta
     3601 gggctcgcct cgctgaagga gggtgcctcg gtcgcggagt tcgccgagtc ccttggtgtg
     3661 ccgtcgagcg agatcgtgaa gatcctcctg ctccgaggca tgccggtcgg tgccgggcaa
     3721 ccaatgccga ccgaccttat ggaggagatt gccgagtcat acgggttcat cgtcgacgtc
     3781 gaggagaccg ccccgccgcc ggcagtcgcc gagcgaccgg agcacgaaga caaagccgat
     3841 gagctgaaac cgcggccacc agtggtgacc gtcatgggcc atgtcgacca cggcaagacc
     3901 acccttctcg acaggatccg caagaccagc gtcgtggacg cagagctggg tgggatcacc
     3961 cagcacatcg gtgcctatca ggtcgaggtc aacgggcacc tggtgacttt catcgacact
     4021 ccgggtcacg aggctttcac ggcacttcga gcccgcgggg caaacatcac cgacatcgtg
     4081 atcctggttg tcgccgccaa cgacggagtc atgccccaga cggtcgaggc catctcccat
     4141 gccaaggccg ccggggtcaa gatcgtggtc gcggtcaaca agatcgatgt gcccggcgcc
     4201 gatccgaccc gggttcggac ggagctgacc gagcacaaca tcatcaccga ggaacttggt
     4261 ggggatgtgc cgagcgtgga agtgtcggcg ctcaatggtg atggtgtcga ggatctcctg
     4321 gaggtcgtcg acctgatcgc ccagctcgag gagtacaagg ccaacccgga cgcccccgct
     4381 tcgggcgtgg tggtggaggc ccgttacgaa accggcaagg gtgcggtcgc aacagtcatc
     4441 atccaacgag gcactctcaa taagggcgac tccttcgtgg ctggcgccgt cgccggtcgg
     4501 gcgcgcggcc tcatggacga gaacgggaac cagatcaagt ccgcaggtcc ctccgacccg
     4561 attcagatca tgggctggga cgatgtcccg gtagccggtg actatttcga ggccgtcgag
     4621 tcggatcgtg aggcacggcg cctggccacc gagcgcgagc aggagctgaa gacgacgagt
     4681 caggctgtcc catcggctcg ggatcggctg cagagcctcc tcgatcagct ccggaccgac
     4741 gaggctcatc tcaatctgat cgtcaaagcc gacgcccagg ggtccttgga ggctctccgc
     4801 gagtccatcg gcaagatcga gcgtgagggt ggaaagatcc agatcgtcca cggcgcggtg
     4861 ggcggcatca atgagaacga tgtcacgctc gccgaggtca ccgagtcggt gatcgtcggg
     4921 ttcaacgtgc gcccggagcc caaggcacgc aaggccgcag aacaggccgg aatcgagatc
     4981 cgcacctacg ggatcatcta cgagatgttg gacgacgtcg agttattgct ggtcggccaa
     5041 ttggcgcctg tcgagcacga gcaggtattg ggcacggccg aggtccgggc gatcttcaag
     5101 gttccccgcg ccggaacggt tgccgggtgt tacgtcgtgg agggtgtgat ccaacgtggg
     5161 gccaaggccc ggctcctgag ggatgggatt gtcattcacg acgggcagat ctccaccctg
     5221 aagcgcttca aggacgatgt gcgcgaagtt gctgccggct ttgagtgcgg catgagcttc
     5281 gagaactaca acgacgtcaa ggagggcgac agcatcgagg cctatctgat ccgcgaggtc
     5341 gctcggacct gatgcgggcc gcggccctga gggtcgagct gcatctgccc ggtccacagt
     5401 cgctcaaggc gaagcgtgcg atcctgcgcc ctgtgaccga gggaatcaag agactggggt
     5461 cattcagtgt ggctgaagtc gatcaccacg acagctggca acgggcggcg ataggtgtcg
     5521 ccatcgttgc ccgagacgga gaaagcctcg ccatgcagat cgcgaagctt cgtcgctacc
     5581 ttgactcccg tttggaagtc gaggttctcg acatccagat gaccgagttg gaggatccgg
     5641 aatgagagag accagcccaa gaatgctcaa ggtcaactca accctgcgtg aggtcctcgc
     5701 tgaggaattg gagcggatga acgacgatcg cctcgagatg gtctcgatca cttcggtcga
     5761 gaccagcccc aatttgaggt cggcgacagt gttcgtcgat gtcctcggtg ccgaggccca
     5821 ggaagccgct ctcgtggcac ttggcagggc ctcgccccgc ctccagaagg ccatcggtcg
     5881 tcaggttcgg atgaagttca cgccacagct cgagttcgcc atcgaccccg gtgtgctcgg
     5941 cggggagcgc atcgagacca ttctccgcaa cctccaacag gatggagacg gagccgaatg
     6001 atcgaccggg ccattctgga gagggctgct gcggccatcg cttctgccga ttccctggct
     6061 cttgcctgtc acatcggccc cgacggtgat gccttgggct cgatgctcgg cttcggaatc
     6121 gctgcggaga acgccgggaa gaaggtcatc gccagtttcg ggtccccctt tgtcgtttcg
     6181 gcaagtctgg cgtttctccc gacacgactt ctggtcccgc cccaggcgtt tccagccgat
     6241 ccaccgatga tggtcgtctt cgacgccggc tcacaggatc gcctcggcga gcttgccgag
     6301 aacgcgtcgc aggccgcgat cacgatcgtg atcgaccatc acatcaccaa cgagggcttc
     6361 ggagacatcg ccgttgtcga cggtgaagcg gcggcaaccg gagagctggt tgcgcatctc
     6421 ctccagatcc ttggctggcc gttgacaccc gagatcgcca cctgcctcca cacggctctc
     6481 gtgaccgaca ccggtcgatt ccaatacggc aacaccacgc cttcgacctt gatgctggcc
     6541 gcagggctgg tggccgctgg cgccgatccg cccgagatca gccgtcatgt gtacgaagag
     6601 gctccctttg gatacctcaa agtggctgga gccgcccttg gccgcgccga gctcgatgaa
     6661 ggtaaagggg tggtcaccgc cgtcgtgacc caagccgatc tcagtgaggc cgggatcgac
     6721 tggggggaca tcgacaatct catcaacacg gtccgactcg cggtcgaagc ggatgttgcg
     6781 gtcctggcca aggtccatgc tgacggcaag gtcaagctca gcctgcgctc cagaggggcc
     6841 accgacgtcg gatcgcttgc cgtggcgatg ggcggtggag gccaccggtt ggcggccggg
     6901 atcacctacg tcggggacgt ggaggacgcc ctcaacgagg tccggcaaag agtcgaggat
     6961 tacaggtgac caagggcttt ctggtggtcg acaaacccgg gggaatcacc tcgaatgcgg
     7021 tggtgggccg ggtgcgaaag gccaccggga tcaagaaggt tggacacgcc ggcactctcg
     7081 accctatggc taccggggcc atggtggtgg caatcggacc ggtgacgagg ttgatccgat
     7141 tcgtccagga gcaaccgaag gaatacctcg ctaccgccca gttcggagtg gccacagaca
     7201 ccctcgatgc cgatggggcg gtgctcgagc gcgagccgat ggacttctcg gaacaggagc
     7261 tcgtggaggc cgccgagaga tttgtcggat cgatcctcca gatcccgccg atggtctcgg
     7321 cactcaagca ccagggccag cgtctctatg agctggcgcg tcgtggcgaa gttgtggagc
     7381 gggaggctcg gcccgtcgag gtctacgagt tggagatcct ctctgtcggc cccggccctt
     7441 atccggatgt cgaattccgc gtcgtttgcg gcaagggcac ctatgtgcgc tcactagcag
     7501 cagatatggc ttcggttcta ggcggaagcg ctcacctcac ggcgctgcgc aggacccgga
     7561 tcggatccct ggaagttgac gtcgtgggtg tggccgtcga cgagctggaa gggtgggaag
     7621 agagcctgtt gacgccttcg caggcgttgt gcgatctgcc ggcggtcacc gtcggcgatg
     7681 acacagccca aggggtgagc aacggactgc gtttcctcgg cggtgagttg gtggacgccc
     7741 ccgatggcgt ggtgcgagtc ctcgacggcg aaggcgagct cctggcgatc tatcggagca
     7801 gcggagccca ggcggtcccc gaagtagtcc tcgcctcatg agggtgctca ccagcccttg
     7861 ggcgagctgg catgcggaga ccgatcccac cgcggtgacg gtcggcgtgt tcgacggggt
     7921 gcatctcggt catcgaacgc tcatcgaccg tctccgggct accgggctcg tgccaactgt
     7981 tctcaccttc gaccctcatc ccgccgaggt gctctcgccg ggcacccacc ctcgtctcat
     8041 cacgagcatc gacgagcggc tggcactact cgagggcgct ggcgtcgagc tggttggagt
     8101 gctcgatctc ggggagattc ggcatctcca tcccggtgag ttcgtgactc aggtgctcga
     8161 agagcgactg gcgatggcgg cgatggtgat gggcaccgac ttccacttcg ggaaggaccg
     8221 ctctggtgac gtggcgttcc tccgcagtgc tggtgacctg aacgggttca aggtcgaagt
     8281 tgtcgagctg cagaccgtcg acggaatcgt ctcatcttct ggaatcaggg cgctgctcga
     8341 gttgggcgag gtgaggaagg ccgaagttct tctcgggtcc cgatacctga tgtccaacgt
     8401 ggtggttcac ggcgacaagc ggggcaggga gatcggcttc cccacagcca atctcgaacc
     8461 tccggaccgc aaggtcatcc cggggatcgg ggtctatgcg gcattcgcct cgctagaggg
     8521 ccagcgccac caggcagcag tgaatgttgg ggtcaggccc accttcggtg gcggcgagct
     8581 ggtggttgag gcgtacctgc tcgatttcaa cgatgattgc tacggggaga cggtgacgct
     8641 cgaattcgta gagcgcttgc gccccgagtt gaagttcgca ggggttccag agttggtgga
     8701 gcaaatgaca gacgatgtcg tgaaggttcg gcacatactg agcgtggagc ccaaaggtgc
     8761 ctgacacggg ccgtcaccgg attccggtgg ggatgccggt tgtgatgaag gctggctctt
     8821 cgagcctggt tcagaggtcg ggggagctcg atgccgaggc cctggccagc acggccgacc
     8881 tcgtcgccgg gatgtgggag ctcggctatc ccaccctcct cgtctcttcc ggggcggtcg
     8941 ctgccggttt gcccgcactc gggctggatc gaagaccgaa ggatgtgcct ggtctccagg
     9001 tcgccgccgc cgtcgggcag ggccggttga tggagcggta ttcggcattg tttgctgaaa
     9061 ggggccgggt ggtgggccag gtcttgctga ccaaggacct cctcgccaac cggagccagt
     9121 atctgcacgc ccgtgaggcc ttggagagga tgctgtcgct aggcgtcgtg cccatcgtca
     9181 acgagaacga cacggtggtt gttgatgggc tcagactggg agacaacgac cgtttggcgg
     9241 cgatcgtctc ccacctggtc tctgccgggc tcctggtgat ccttactgac accggtggga
     9301 tctactccgc cgacccgagg actgtggcgg atgcggagtt gctgagcgcg gtccggccca
     9361 ccgacgccgc gttggacgtg gtcgtctccg gcgagccggg ccccttcggc tccggtggcg
     9421 ccgccaccaa ggttgccgcg gcccggatgg cggcgtggtc tggcatcccc acagtgatcg
     9481 ccgatgcccg agaaggtgac gtcctggccc gcgccgcagc tggcgaggag atcggaacct
     9541 gggtcacccc ccatgagacc aagctcccgg cccgcaagct gtggatcgcc tttggccagc
     9601 cgacggaggg caaggtcgtc gtcgatcccg gtgcgaccaa ggcattgctg gaaggtggca
     9661 agtcgctgct ggcggtaggt gtgttgtcgg tcgacggtgc cttcgacgcc gaaggggcgg
     9721 tcgaggtctt cgacggcgat ggccgcctcg tcggcaaggg cctgacgacg atgagcgcag
     9781 atcaggcccg tcaatccgtt ggccggcaca gctctgaggt gggcggtgtg gtcatccatc
     9841 gtgacgattt cgtcctcctc gcggttgagc aatcagcgcc tgtcgatagg cttgccccat
     9901 gattgacgtg atcggcaagc gggcacgaga cgcctcacgt gagctcgctc agtccccggg
     9961 ggaggtgcgg gagcatgccc tgaggcagat ggccgaggcc ctcgagaaag ctacctctgc
    10021 tctactcgtc gccaacgaag ccgacctcga acaagcggcc ggtgagggga tcgggggagc
    10081 ccttctcgac cgtcttcggc tgaccgaggc cagggttcgc ggcatggctt ccggtctgca
    10141 gacggtcgca gctttgccgg atccggtcgg tcgggaaacg gggggatggg tgctgcacaa
    10201 cgggatccgc ctccatcgag ttcgggcgcc gcttggagta gtggccgtga tctatgaggc
    10261 cagacccaat gtcaccgccg acgcggccgg gctctgcatc aagtccggca acgccgtgat
    10321 ccttcgcggc tcctcgtatg ccctccggtc aaacatggca atcagcaacg tgttgcgaga
    10381 ggccctttcc aacattgggc tgcctggcga cgccgtccag ctcctggagg acacctcgcg
    10441 ggatggggcc aaggccttga tgcaggccac cgagtgggtg gacctcctga tcccgcgtgg
    10501 cggtccgggt ctgatcgacg cggtgcgagc cgatgccacc gtgcccacgg tcatcgacgg
    10561 ggcgggcaac tgccatgtgt atgtggatgc ggcggcggat ctggacatgg cccgatcgat
    10621 agttgtcaac gccaaggtgc agcgcccggg ggtatgcaat gcggctgaga ccctgctggt
    10681 gcatgaagcg gttgccgacc aattcctctc cgatgtggcc aaggctctcg tcgccgaggg
    10741 agtcgagctc aggggagacc agcgttcccg tgagctcgtg cccgagatgc tcgaggcctc
    10801 cgatgacgac tgggcgaccg agtttcttga cctgataatg gcagtgaagg tggtccccga
    10861 cgtcgacgcc gcgatcgacc acatccggag gttcggtacg ggtcacaccg aggcgatcgt
    10921 gaccgaggac cgctctgctg ccgacagatt cgtgggcggc atcgacacgg cggtcgttgc
    10981 cgtcaacgtt tcgacgagat tcaccgatgg cgaggagttc ggattcggcg ccgagatcgg
    11041 gaactcgaca cagaagctgc atgtgcgggg cccgatgggg ctcgaatctc t
//
LOCUS       DBNHXU010000094         2015 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2211565, whole genome shotgun sequence.
ACCESSION   DBNHXU010000094 DBNHXU010000000
VERSION     DBNHXU010000094.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2015)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2015)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2015
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2211565"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="AC161X_02540"
     CDS             <1..867
                     /locus_tag="AC161X_02540"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005255260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR4988459.1"
                     /translation="WVYDHFHTVPIATQEETYEAWSLMAALAAVTSRIRLGQMCTCNS
                     YRHPSYLAKVAASIDVISGGRVEMGIGAGWYEEEYVGYGYEFPKPSIRLGQLDEGVQI
                     MQRLWTEDVVHFEGRHYELDGAICRPKPIQEPHIPIWVAGGGEQLTLRTAARYAQYTN
                     FGIDLDSFIHKSHVLRDHCQAEGTDYEAIVRSKNFNIICAATEAEVDSKLAWLKEKYL
                     RYVPESRAQSQVDEFRRMSGTPEQIVEKLEEWESAGMTYAIIYFPDPAYDTSGLELFS
                     KEVIPALGSVLN"
     gene            complement(853..>2015)
                     /locus_tag="AC161X_02545"
     CDS             complement(853..>2015)
                     /locus_tag="AC161X_02545"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4988460.1"
                     /translation="GKTAFELDASATGVAAVMAALGLASLIGSAIAGVLVDRYDPRRV
                     LIWGELLFIPVALSMVFANTIPTMGVATFALGLVGTPVYTAIASFGPYLTDDPEQLAK
                     VNGYIEGASWAAFVVGPALGAVITITVGLNGIFVLDAATSAIAVVLMIPVTVRKLEKK
                     EQDGSHGFQELREGFRYSYRSRRLRFYLILGSLVWILFGTFSALEPLFYRDVLGVAPE
                     TMGWVNSLFGIGLVTGTLISSRIPPRLRTARTMVVLVALNSVGDLLYIGTNRLPVVVA
                     AGMTWGVVIGTMAPLLRTMIQINSPEDLVGRITGVSQVHSEVAHLMPLAFAPLLAASF
                     GVQPSLMIAGVFVGLLALCFYPEARKLDQMTELAVPRPGFPDPSTEPKSVQH"
BASE COUNT          443 a          624 c          611 g          337 t
ORIGIN      
        1 tgggtctacg accatttcca caccgtcccg atcgccaccc aagaggagac ctacgaggct
       61 tggtccctga tggcggcact tgccgccgtc accagcagaa tccgactcgg ccagatgtgc
      121 acctgcaact cgtatcgcca tccctcatat ctcgccaaag tcgcagcgtc gatcgatgtc
      181 atctccggtg ggcgggttga gatgggcatc ggcgccggct ggtacgagga ggagtacgtc
      241 ggctacggat acgaattccc caagccatcg atccgtctcg gccagctcga cgaaggcgtc
      301 cagatcatgc agcgactgtg gaccgaggac gttgtccact tcgaaggtcg tcactatgag
      361 ctcgacggag caatctgccg gccaaagccg atccaagagc cacatatccc gatctgggtg
      421 gccggtgggg gtgaacagct gactttgcgg actgcggcgc gctacgccca gtacaccaac
      481 ttcggaatcg acctcgattc cttcatccac aagtcgcatg ttctccggga tcattgccag
      541 gccgaaggaa ccgactacga ggcgatcgtt cggtccaaga acttcaacat catctgcgcc
      601 gccaccgagg ccgaagtcga ctccaaactc gcctggctga aagagaagta tctccgctac
      661 gtccctgaga gccgggctca gtcacaggtc gacgaattcc gccggatgtc tggcacgccg
      721 gagcagatcg tcgagaagct ggaggaatgg gaaagcgccg gtatgaccta cgccatcatc
      781 tacttccccg accccgccta cgacacaagc gggctcgagc tgttctccaa agaggtaatc
      841 ccggcccttg ggtcagtgct gaactgattt tggctcggtg ctcgggtcgg ggaacccagg
      901 gcgaggaact gcgagctccg tcatctggtc gagcttgcgg gcttcgggat agaagcagag
      961 agcgagcaac ccgacgaaga cgccggcgat catcaaggaa ggctgcaccc caaacgacgc
     1021 tgccaacaat ggcgcgaacg cgagaggcat gagatgggcc acctcggagt ggacctggga
     1081 gacaccggtg atccgcccaa ccaaatcctc gggtgagttg atctgaatca tggtccggag
     1141 gagaggggcc atcgtcccga tcacgactcc ccaggtcatc cctgcggcca ccaccaccgg
     1201 aagccggttg gtgccgatat agaggaggtc gcctaccgag ttgagggcga ccaggaccac
     1261 catcgtgcga gctgtccgga gccgcggcgg tatccgcgag gagatcaaag ttccggtcac
     1321 gaggccgatg ccgaacaacg agttgaccca tcccatggtc tcgggtgcga ccccgagcac
     1381 atcgcgatag aagaggggct ccaacgcact gaaggtccca aagaggatcc agaccaggga
     1441 cccaaggatc agatagaacc gcagccggcg gcttctgtac gagtagcgga atccctcccg
     1501 gagctcctgg aaaccgtgtg aaccgtcttg ttccttcttt tcgagcttgc gcacggtgac
     1561 cgggatcatc agcaccacgg ctatcgcaga ggtagcggcg tcgaggacaa agatcccatt
     1621 gaggccaacc gtgatcgtga tcacggcccc gagcgccggg ccgacgacga acgccgccca
     1681 ggatgcgcct tcgatatagc cgttgacttt cgccagttgc tcggggtcgt cggtgagata
     1741 cgggccaaac gatgcaattg ccgtgtagac gggggtcccg accagcccga gggcgaaagt
     1801 ggccaccccc atcgtgggga tggtgttggc aaacaccatg gatagtgcga ccgggatgaa
     1861 tagcagctca ccccagatca agacccggcg cgggtcatat cgatcgacca gaactccagc
     1921 aattgccgag ccgatcaacg atgccaggcc cagggcggcc attactgcgg ccacaccggt
     1981 tgccgaagcg tccaactcga aggcggtttt gcccc
//
LOCUS       DBNHXU010000095         1793 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2212409, whole genome shotgun sequence.
ACCESSION   DBNHXU010000095 DBNHXU010000000
VERSION     DBNHXU010000095.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1793)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1793
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2212409"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..311)
                     /locus_tag="AC161X_02550"
     CDS             complement(<1..311)
                     /locus_tag="AC161X_02550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988461.1"
                     /translation="MSDSLSTFLDLLTRTDFLVGGFLGLTGLVVIYFVRTDEGPVLEW
                     GLTLTVAAFLAVSLAVGSRLSLLASLFALAVGGWMARPPGGSARAPFGWILIAAGAIT
                     MV"
     gene            442..1596
                     /locus_tag="AC161X_02555"
     CDS             442..1596
                     /locus_tag="AC161X_02555"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4988462.1"
                     /translation="MRVLHVIDSLAASGGAEQGLVREVTRFSPGTEQRVVTLYDRMEL
                     ADKLSEREIPVQTIGLEEGTGSRSWLRATTAVSRIAQEFKPDVIQTSLFLGNIVGQLA
                     GRRRGVPVVSNLVLSGDVESLRAYQPGASTRRAGWLRSIAGWAARSDLVRFRALTEEV
                     RTSNAVLLGVDPSLVTVIPRGVPRPPEVSRRSPAELGLREGPLAVNVGRLAPQKGQVL
                     LVEAFAQVIEHVPDARLAIVGRDGEASDQVSEAIESFALKGSVTLVGHTSKVVDYLAA
                     AHVFAFSSLMEGLGTAVLEAMAVGVPVVAFDIPPVREATADGRFATLVPVGDVAELAT
                     ALVDHLSKEKRIDTEARDWVADHHDLDRIAGQVEALLASAAASSAPADAR"
     gene            1609..>1793
                     /locus_tag="AC161X_02560"
     CDS             1609..>1793
                     /locus_tag="AC161X_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012827123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HIR4988463.1"
                     /translation="MARIYLSPPDVGLEDRSAIQRAVESGWIAPLGPEVDGLETELAA
                     ATGRDHGVALSSGTAAL"
BASE COUNT          309 a          565 c          597 g          322 t
ORIGIN      
        1 accatggtga tcgccccggc tgcgatcagg atccagccga agggagcccg agcactgcca
       61 cccggtggtc gggccatcca accaccgacg gctagagcga acagcgatgc cagcaaggac
      121 agtctcgacc ccacggcgag gctcacggcg agaaaggccg caacggtgag cgtgagcccc
      181 cattcgagca ccgggccttc gtcggtccga acgaagtaga tcacgaccag cccggttaac
      241 ccgaggaagc caccgaccag gaagtcggta cgcgtcagaa ggtcgagaaa agtcgagagg
      301 gagtccgaca tcgggtggga tcgtaagact tgcttccctc ctaccggatc cattcggatc
      361 tggcttggcg tggcgtcacc ggccgtggac agaggtccca ctaccctcgg gcatgcgata
      421 aatccccatt cgaagggcca aatgagggtc ttgcacgtca tcgacagcct tgcggcgtcc
      481 ggtggtgccg agcagggtct ggtgcgtgag gtgaccaggt tctcccccgg cacggagcaa
      541 cgcgtcgtga cgctgtatga ccggatggag ttggcggaca agctctccga acgggagatc
      601 ccggtgcaga ccatcgggct cgaggagggg accgggtcac gatcctggct ccgcgccacg
      661 accgccgtca gccggatcgc tcaggagttc aaacccgacg tgatccagac cagccttttc
      721 ctgggcaaca tcgtcgggca actggcggga cgcaggcgtg gggtccccgt cgtctccaat
      781 ctggtgctgt ccggcgacgt cgagtctttg cgcgcctatc agcctggcgc ctcgacccgg
      841 cgggccggct ggctccgctc catcgctgga tgggctgccc ggagtgatct cgtccgcttc
      901 agagccctga ccgaagaggt gaggaccagc aacgcagtct tgctcggggt cgatccgtct
      961 cttgtcacgg tcatccctcg aggcgtgcct cgtccgcccg aggtgtcccg aaggtctcct
     1021 gccgagctgg gactccgcga ggggccgttg gcggtcaatg ttggccgtct ggcgccacaa
     1081 aaaggacagg ttctcctggt agaggctttc gctcaggtca tagagcatgt gccagacgct
     1141 cgtttggcga tcgtcgggcg tgatggggag gccagtgatc aggtgtcaga ggccatcgag
     1201 tcattcgcgt tgaaaggctc ggtcaccctg gtgggacaca cgtcgaaggt ggtcgactat
     1261 ctcgcggcgg ctcacgtgtt cgctttctct tctttgatgg aagggctggg gaccgcggtc
     1321 ctggaggcga tggcggtcgg ggtcccggtt gtcgccttcg acattccccc ggtcagagag
     1381 gcaacggccg acggtcgttt cgccactttg gtcccggtgg gggatgtcgc cgagctcgca
     1441 acggcgctgg tggaccacct ttccaaggag aagcggatcg acaccgaagc tcgggattgg
     1501 gtcgcagacc atcatgatct ggaccgaatc gccggacagg tcgaggctct tctcgccagc
     1561 gctgccgcat cttccgctcc agcggatgct cgttagcctc actcagacat ggccaggatc
     1621 tacttgtcac cgcccgatgt tggcctcgag gaccggtctg ccatccaacg cgccgtcgag
     1681 tcgggttgga tcgcccccct ggggccggaa gtcgatggcc tcgagaccga gctcgctgcg
     1741 gccaccggga gggaccacgg tgttgccctc tccagtggga ccgccgccct cca
//
LOCUS       DBNHXU010000096         5438 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3799438, whole genome shotgun sequence.
ACCESSION   DBNHXU010000096 DBNHXU010000000
VERSION     DBNHXU010000096.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5438)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5438)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5438
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3799438"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..858
                     /locus_tag="AC161X_02565"
     CDS             <1..858
                     /locus_tag="AC161X_02565"
                     /inference="COORDINATES: protein motif:HMM:NF020073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HIR4988464.1"
                     /translation="RDLRILGVDPSRNLSEIATATGVPTIDEFFTEDLATRIVETHGQ
                     ADLIVGCNVLGHIDDLDDVCRGVKALLGDDGAFVIEIPYLGAMLDRLEWDTIYHEHLS
                     YFAVAPLRHLFTRFGLGLREVEFVPVHGGSIRCTVVHGDVTAPSIDDLIEAETAKRLA
                     DRRTYAPLPDMVTGHQRALRSWLEEQGSEGRTVIGYGAPAKGSVLLNSCRIGPELLSV
                     VLDSTPAKQGLHMPGTHQPILPPGALEEIGPDILLVLAWNHASEIAEREHHFRAGGGR
                     LLTPYLDEL"
     gene            complement(868..1836)
                     /locus_tag="AC161X_02570"
     CDS             complement(868..1836)
                     /locus_tag="AC161X_02570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4988465.1"
                     /translation="MNSTVTIGIPVYHGEEFLKETVDSLLAQTFSAWRAAFSIDGPDL
                     ASEDVCRQYLADPRFSLTIQPERLGWVRNISWLQRQADTEFWCYQQQDDLLHPSYLEM
                     LIEEANRRPGAAVVYCDIETFGDRTQRFEGESVVGSPLNRQLSLLTDHFAGVAFRGLT
                     RVSALVETGGGLVENDVDNFAAETVWIATMATWGDLVRVPATLYRKRYHANNAHAVWL
                     SWDRSQRVHAWTTHCHDILEVAMRIAESRAECWLLWMTTLNRLTASRATSYLPWADLA
                     DEDRAAMIDELLERTRRLARIDLDTLLGASWRVIGRRSHQFIAAGV"
     gene            complement(1833..3053)
                     /locus_tag="AC161X_02575"
     CDS             complement(1833..3053)
                     /locus_tag="AC161X_02575"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR4988466.1"
                     /translation="MKAGVRVAVLGAGLQGSCLAMDLASTGFLVDLFDRNPACMTQAS
                     LHNEGKVHLGYVYGNDPSLRSARAMVEGAATFGPLLERWLEIPMSRLGRSAEFNYAVH
                     RDSLLSPADFEVHLRRTHEIVVGRIAPGDYFGLDLHQPPRRLRAPELENDYDPQVVQT
                     VHRTSEVSIDPRVLAQTVRDRIAADHRITQVLDTEVVGVGLADDRITVEFIDRGTPAV
                     ADYEYVVNALWDGRLVVDASAGLVPPNPWLFRVKHFLMLQSATARLPSTTIVLGPFGD
                     IVDYGQGAFYLSWYPSGLQGSSSGLEVPESLRSLPGEASHEMRAGIVAGLASITPRLL
                     EVPVSDMDAAELSGGVIFAWGETDIDDPSSGLHSRFETGPMSVGRYHSVDTGKLTTAP
                     LFAQRVGDRLRAAQ"
     gene            3130..3678
                     /gene="rfbC"
                     /locus_tag="AC161X_02580"
     CDS             3130..3678
                     /gene="rfbC"
                     /locus_tag="AC161X_02580"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019651487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HIR4988467.1"
                     /translation="MQATATSLSGLLILEPEIFEDHRGFVVESWNRRVFEETTGLSPE
                     FVQDTVSGSRRGVLRGLHYQIPPDAQGKLVRTARGSVFDVAVDLRRSSPTFGGWFGIE
                     LSEANHKQLWIPAGMAHGFAITSDWAEVHYKSTAHYSRASERGLRWDDPEVGIEWPLD
                     TPPLLSEKDTKAPVLQDAEVFA"
     gene            3698..4675
                     /gene="rfbB"
                     /locus_tag="AC161X_02585"
     CDS             3698..4675
                     /gene="rfbB"
                     /locus_tag="AC161X_02585"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011033116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009225 - nucleotide-sugar metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HIR4988468.1"
                     /translation="MGTERRYLVTGGAGFIGSNFIRYLLESEPDARVTNLDALTYAGV
                     TATVEELEASSRLTFVHGDIRDLILVDRLMNDHEIVVHFAAESHVDRSISDPSVFLET
                     NVVGTGLLLDAARRANVSRFIHVSTDEVYGSLDHGYAAENAPLRPSSPYSSSKAAADL
                     VALSYANTFDFPVTVTRSTNNYGPYQFPEKLIPLFITNLIEGKTAPVYGDGSNRRDWL
                     FVNDHCAAIHLLVDEGGRGEVYNIGAGSELSNLEVARSIIASLGQDETRIEFVEDRAG
                     HDFRYGVDFSRIRDLGWAPSGNFDDRLTETISWYQSRQDWWGPIKALQP"
     gene            4672..>5438
                     /gene="rfbA"
                     /locus_tag="AC161X_02590"
     CDS             4672..>5438
                     /gene="rfbA"
                     /locus_tag="AC161X_02590"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014960469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HIR4988469.1"
                     /translation="MKGIVLAGGAGTRLDPITRVVSKQLQPVYDKPMIYYPLATIMEA
                     GIREIMMITTPKDAPLFKELLGSGEQFGIDLRYEAQPEPRGIAEAFVIGQDFIGGDPV
                     TLILGDNVFYGATGLEAIVRDLTQGAVIFGYPVRDPERYGVVEFDDSGRVLGVEEKPS
                     RPRSKYAIPGLYVYDDQVVEIARAVRASERGEKEITDVNRVYLEQGQLRVSLMDRGVA
                     WLDSGTHDSLLDAANFVATVEHRQSIKIACLEEVAFRA"
BASE COUNT         1069 a         1708 c         1652 g         1009 t
ORIGIN      
        1 cgcgacctcc gcatccttgg agtcgacccc tcacgcaacc tctccgaaat cgctaccgcc
       61 accggcgttc cgaccatcga cgagttcttc acagaagacc tggccacgag aatcgtcgag
      121 actcatggac aggccgatct gattgtggga tgcaacgtgc tcggccacat cgacgacctc
      181 gacgatgttt gccgtggagt caaggcattg ttgggcgacg atggcgcttt cgtcattgag
      241 atcccctatc tcggtgccat gctcgatcgg ttggaatggg acaccatcta ccacgagcat
      301 ctgtcctact tcgcggtcgc tccccttcga catctcttca ctcgtttcgg gctcggtctg
      361 cgtgaggtcg agttcgttcc cgttcacggt gggagcattc gatgcacagt ggtccacggc
      421 gacgtcacgg caccatcgat cgatgacctg atcgaggccg aaaccgccaa gcgcctggct
      481 gacaggcgga cctatgcccc gctccccgac atggtcaccg gtcaccaacg tgcattgcgc
      541 tcgtggctag aagagcaagg cagtgaaggg cggacagtca tcggctatgg agcaccggca
      601 aagggaagcg tgctcctcaa ttcgtgcagg atcggccctg aattgctctc agtggtgctg
      661 gactcgacgc cggcaaagca agggctccac atgcccggca cccatcagcc catcctcccg
      721 ccgggggctt tggaagagat agggcccgac attctcttgg tcctggcatg gaaccacgcg
      781 agtgagatcg ccgaacggga gcatcacttc cgggctggag ggggacgcct gctcacgcca
      841 tacctggacg agttgtagga accgctctca gacgccggca gcgatgaact ggtgcgagcg
      901 acggcctatg acccgccaac tcgccccgag cagcgtgtcg agatcgatcc gggcaagtcg
      961 ccgggtgcgc tccaacagtt cgtcgatcat ggcggccctg tcctcatccg ccagatcggc
     1021 ccaaggcagg taggaggtgg cccgcgaggc cgtcagacga ttcagggtcg tcatccacag
     1081 cagccagcat tcggcccgcg actccgcgat ccgcatcgcc acttcgagga tgtcgtgaca
     1141 gtgggtggtc cacgcgtgca cccgctgaga cctgtcccat gacaaccaaa ctgcgtgagc
     1201 gttgttggcg tgatagcgct tgcggtaaag agtggcgggc acccggacca agtcacccca
     1261 ggtggccatg gtggcgatcc agactgtctc cgcggcgaaa ttgtctacat cgttctcgac
     1321 cagcccgccg ccagtctcga ccagggcact gacccgggtc aggccccgga aggccacacc
     1381 ggcgaaatgg tcggtgagga ggctgagctg acggttcaat gggctgccaa cgacggactc
     1441 tccttcgaag cgctgggtgc ggtctccgaa ggtctcgata tcgcaataga cgacggcggc
     1501 gccgggtcgg cgattggcct cctcgatcag catctcgagg tacgacggat ggagcaggtc
     1561 gtcctgttgt tggtagcacc agaactcagt gtcagcctgg cgctgtagcc aactgatgtt
     1621 gcgcacccat ccgagccgct cgggctggat cgtgagactg aaacgcggat ccgccaggta
     1681 ctggcgacaa acgtcctctg aggccaggtc cgggccgtcg atcgagaatg cggcgcgcca
     1741 tgccgaaaag gtctgggcga gcagcgagtc gacggtttcc ttcaagaact cctcaccgtg
     1801 atataccggg atcccgattg tcaccgtgga attcactggg cggcccgcag cctgtcgccc
     1861 accctttgag caaacagcgg ggcagtggtc agtttgccgg tgtccacgct gtggtatcgg
     1921 ccgaccgaca ttggcccggt ttcaaatcga ctgtgtaggc cgcttgaggg gtcgtcgatg
     1981 tcggtttcac cccaggcaaa gatgacacct ccggacagct cagcagcgtc catgtcggaa
     2041 accggaactt ccaacagcct gggcgtgatc gaggcgaggc cagcgacgat tcctgccctc
     2101 atctcatgcg aggcttcacc gggcaagctc cgcagcgatt ccgggacttc caagcccgag
     2161 gatgacccct gcaagccact cggataccac gagaggtaga aggctccctg gccgtaatcg
     2221 acgatgtccc caaacgggcc gagcacgatc gttgtggaag gcagccgtgc ggttgcggac
     2281 tgcaacatca ggaaatgctt cacccgaaac agccaggggt tgggcggaac caggccggcg
     2341 gaggcatcca cgaccagccg accgtcccaa agggcattca ccacatactc gtagtcggca
     2401 acagcgggag ttccgcgatc gatgaactcg actgtgatcc ggtcgtcggc aaggccgacg
     2461 ccgaccactt cggtatcgag cacctgggtg atgcgatgat cggcagcgat ccgatcacga
     2521 actgtctggg ccagaactct ggggtcgatg ctgacctcgc tggttcggtg caccgtctgc
     2581 accacctgcg ggtcgtagtc attctcgagt tcgggcgctc gaagccggcg cggtggctgg
     2641 tggaggtcga gcccgaagta gtcacctggg gcaatgcgcc cgacgacgat ttcgtgggta
     2701 cgacggaggt ggacctcgaa atcggccggg gaaaggaggc tgtcccggtg cactgcgtag
     2761 ttgaactcgg cggaccgtcc gagtcgcgac atcgggattt cgagccagcg ctcgagaagc
     2821 ggcccgaacg tggcggcccc ctccaccatc gctctcgccg agcgcaggct cgggtcgttg
     2881 ccgtatacat atccgaggtg gaccttgccc tcgttgtgga gggatgcctg ggtcatgcac
     2941 gcgggattcc tgtcgaagag atccaccaga aagccggtac tggccaggtc catcgccaga
     3001 caactgcctt gtagcccggc tcccagcacc gcaacccgca caccggcctt caaggtgtca
     3061 tcacgtcggt gagacagtaa ttatcgtgag caggcaactc gatttacccc accggtaccc
     3121 tgcgccgcga tgcaagccac ggcgacctcc ctctccggct tgctcattct cgagccggaa
     3181 atcttcgaag accatcgagg cttcgtcgtc gagagttgga accgtcgcgt cttcgaggag
     3241 acaactggcc tgagccccga gttcgtccag gacaccgtgt cgggctcgag acgcggcgtg
     3301 ctgcgcggcc tgcactatca gatccctccc gacgcccaag gcaaactggt gagaacggcg
     3361 cgcggatcgg tgttcgatgt cgcggtcgac ctccgccgct catcaccaac attcggtgga
     3421 tggttcggca tcgagctctc ggaagccaac cacaagcagc tttggattcc ggcgggcatg
     3481 gcccacggtt tcgccatcac ctcggactgg gccgaagtcc attacaagtc gaccgcccac
     3541 tactcgcggg cttccgagcg cggtctgaga tgggacgatc cggaggtcgg aatcgaatgg
     3601 cctctggata ccccacctct cctctcggag aaggacacca aagctcccgt gcttcaggac
     3661 gccgaagtct tcgcataggt tcctcgacgg agattccatg ggaacagaac gccgctattt
     3721 ggtcacgggc ggagccggct tcatcggttc caacttcatc aggtatctat tggaaagcga
     3781 gcccgacgcc agagtgacca acctggatgc gctcacatac gccggcgtga cggccaccgt
     3841 cgaggaactc gaggcctcga gcaggctcac cttcgttcac ggcgacattc gtgacctgat
     3901 cttggtcgat cgtttgatga acgaccacga gatcgtggtc cactttgcgg ccgaaagtca
     3961 tgtcgaccgg tcgatcagcg acccatccgt cttcctcgag accaatgtcg tgggcacagg
     4021 gctcctcttg gatgccgcaa gacgggcgaa cgtttcaagg ttcatccacg tgtctaccga
     4081 cgaggtctac ggatccttgg accatgggta cgccgctgag aacgccccac ttcgaccgtc
     4141 ctccccgtat tcgtcgtcca aggcggcggc cgacctggtc gccctgtctt atgccaacac
     4201 gttcgacttc ccggtgacgg ttacccgcag caccaacaat tacggtccct atcagttccc
     4261 ggaaaagctg atccccttgt tcattaccaa cctcatcgaa gggaagaccg ctcccgtcta
     4321 cggcgacggc agcaacaggc gcgactggct gttcgtgaat gatcattgcg ctgccatcca
     4381 cctcttggtc gacgaaggcg gacgaggcga ggtctacaac atcggggctg ggtccgagct
     4441 ctcaaacctg gaagtagctc ggagcatcat cgcttcgctt ggtcaggatg agacccggat
     4501 cgaattcgtg gaggatcgag ccggccacga cttccgctac ggagtcgact tctcgcggat
     4561 acgcgatctg ggatgggccc cgtctggcaa cttcgacgac cgcctgacag agaccatctc
     4621 ttggtaccaa agtcgacagg attggtgggg tcccatcaag gccctgcagc catgaaggga
     4681 atcgttcttg ccggcggagc gggaactcgc ctggacccga tcacgagagt cgtctccaaa
     4741 caactgcagc ctgtgtatga caagcccatg atctactacc ccctggccac gatcatggag
     4801 gccgggatcc gagagatcat gatgatcacc acccccaaag acgctccgct gttcaaggag
     4861 ctcctcggct ctggcgagca attcggcatc gatctgagat acgaggccca accagagcct
     4921 cgaggcatcg ccgaggcctt cgtcatcggc caagacttca tcggcggcga cccggtgacg
     4981 ctgatcctcg gcgacaacgt cttctatgga gcgacaggtc tcgaagccat cgtccgcgac
     5041 ctcacccagg gtgcggtcat cttcggttat ccggtgcggg acccggagcg ctacggggtc
     5101 gtcgagttcg atgactccgg ccgagtcctt ggcgttgaag agaagccgag cagaccccgt
     5161 tcgaagtatg cgattccagg tctctacgtg tacgacgacc aggtggtcga aatcgctcgt
     5221 gcggttcgtg catcagagcg aggcgagaaa gagatcaccg atgtgaaccg ggtctacctg
     5281 gagcagggcc agctccgcgt ctccctgatg gaccgcggag ttgcgtggct cgatagcggt
     5341 acccacgaca gccttttgga tgccgccaac ttcgtggcca cagtcgagca ccgccagtcg
     5401 atcaagatcg cctgtctgga ggaggtggca ttccgggc
//
LOCUS       DBNHXU010000097         2296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2608047, whole genome shotgun sequence.
ACCESSION   DBNHXU010000097 DBNHXU010000000
VERSION     DBNHXU010000097.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2608047"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1180)
                     /locus_tag="AC161X_02595"
     CDS             complement(<1..1180)
                     /locus_tag="AC161X_02595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988470.1"
                     /translation="MTGILQPWKSSDMILTDGRPGRFRIPSVWIGLVVGVVLLQWLAR
                     SDTFPALWDTVFSDPIDQFRDWAQANRQIHPIFTAFFIPVSTSIEWGLTTIESFLLWL
                     PWFVLPLSVFLVIFRSGDLKTAIIATLAMIYPGLIGLWEPTMQTLSLMTIAVLISLAI
                     GIPLGVWAARSSRVERLIRPFLDAMQTIPAPVYFIPIVLFFGIRRVPATIATIIYALP
                     PAVRLTTLGLQGVPRQSVEAAEMFGSTRRQTLFKVQLPMAMPSIMTGINQTIMMALGI
                     VVIASLVGAGGLGQEVLDGLTKRSTGRALAAGLGIVAVAIVLDRVSRSFAFADRTRPA
                     SRRYVTMSLAAVAGAIVVGRVFGWVEFPAIWDVRLFDPIDDFVLWGRDNLSFITRPVN
                     D"
     gene            complement(1177..2211)
                     /locus_tag="AC161X_02600"
     CDS             complement(1177..2211)
                     /locus_tag="AC161X_02600"
                     /EC_number="7.6.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003998664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015697 - quaternary ammonium group
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quaternary amine ABC transporter ATP-binding
                     protein"
                     /protein_id="HIR4988471.1"
                     /translation="MGFSNEPPEDVCLSVEHVWKVFGAHPERAIELAEGGASRAEVRE
                     ATECTIGVRDVSFNVGRGETFVVMGLSGSGKSTLIRCVARLNDVTRGQVYLDGEELTA
                     MSDGQLREVRRHKMSMVFQHFGLFPHRRVIDNAAYGLEVQGVEKAERYERAMKVLDVV
                     GLQGCENYFPQELSGGMQQRVGLARALTVDPEILFFDEPFSALDPLIRRDMQDELIRL
                     QETLQRTIVFITHDFAEAIKLGDRIAIMKDGAFDQVGTAYELITHPATEYVQEFTKEI
                     PKAKVLTAGAVMKPGTGAPNGKTVSLDDTIETVLPALLHDSEEISVEDGEGNLVGVVD
                     RDAVANALES"
BASE COUNT          482 a          698 c          714 g          402 t
ORIGIN      
        1 agtcattgac agggcgagtg atgaaggaga ggttgtcccg cccccagagc acgaagtcgt
       61 cgattgggtc aaagagcctg acgtcccaaa tcgcaggaaa ctcgacccaa ccgaagaccc
      121 gaccaaccac gatggctccg gcgaccgccg ccagggacat ggtgacgtaa cggcgggatg
      181 ccggtcgggt gcgatcggcg aaggcaaagg agcgactgac ccgatcgagc acgatcgcca
      241 ctgcaacgat ccccaagccc gcggccagcg cccggcccgt cgaccgttta gtgaggccgt
      301 cgagcacctc ttggcccagc ccgccggctc cgaccaggct ggcgatgacc acgatcccca
      361 gcgccatcat gatcgtctgg ttgatcccgg tcatgatcga gggcatcgcc atcggcagct
      421 gcaccttgaa cagggtctgc cgtctggtgg atccgaacat ctccgccgcc tccaccgact
      481 gccggggcac accttgaagc ccgagtgttg tcagccggac cgcggggggc agggcgtaga
      541 tgatggtggc aatggtggcc ggcaccctgc gaatcccgaa gaagagaacg atcggaatga
      601 agtagacggg cgccgggatt gtctgcatcg cgtcgaggaa tggccggatg aggcgctcca
      661 cgcgtgaaga gcgagcggcc caaaccccga gggggatgcc gatcgcaaga ctgatcagca
      721 ccgcgatcgt catcagggag agggtctgca tcgtgggctc ccagaggccg atcaagccgg
      781 gatagatcat cgccagcgtg gcgatgatcg ctgtcttcag gtcaccggac cggaagatca
      841 ccaggaaaac ggacaggggc aacacgaacc atgggagcca cagcagaaat gactcgatgg
      901 tggtcagtcc ccattcgatg gaagtggata cagggatgaa gaatgcggtg aaaatcggat
      961 gaatctggcg attggcttga gcccagtcgc ggaattggtc gatcgggtcc gagaagacgg
     1021 tgtcccaaag ggcgggaaac gtgtccgacc gagccaacca ttgcagcagg acaacgccga
     1081 caacgagacc gatccatacc gatggaatgc ggaatcggcc tgggcggccg tccgtcaaga
     1141 tcatgtccga ggacttccag ggttggagga tcccggtcat gactcgagtg cgttcgccac
     1201 cgcatctcga tcgactactc cgacgagatt gccttctcca tcctctaccg agatctcctc
     1261 cgagtcgtgg aggagtgcgg ggagcacggt ctcgatggtg tcgtcgagac tgaccgtctt
     1321 tccgttgggt gctccggttc caggcttcat caccgcacct gccgtgagca ccttggcctt
     1381 gggaatttcc ttggtgaatt cctggacgta ctccgtcgcc ggatgggtta tcagctcgta
     1441 agcggtcccc acctgatcga aggcaccgtc cttcatgatt gcgatccgat ccccgagctt
     1501 gatcgcctcg gcgaagtcgt gggtgatgaa gacgatggtc cgctgcagcg tctcctggag
     1561 tcggatcagc tcgtcctgca tgtcgcgtcg gatcagcggg tcgagtgccg agaacggctc
     1621 atcgaagaac aggatctcgg ggtccacggt caaggcccgc gccaggccca cccgttgttg
     1681 catcccaccg gatagttctt gtgggaagta gttctcacat ccctggagcc ccaccacgtc
     1741 gagaaccttc atcgctcgct cgtaacgctc ggccttctct accccctgca cctccaagcc
     1801 ataggcggca ttgtcgatga cccgacgatg tgggaacagg ccgaagtgct gaaagaccat
     1861 cgacatcttg tggcggcgga cctcgcgcaa ttggccgtca ctcatcgcgg tcagctcctc
     1921 gccgtcgagg tagacctggc ctctggtgac gtcgttgaga cgggcaacgc agcgaatcag
     1981 agtggacttg cccgacccgg agagccccat gacaacgaag gtctcgcctc ggcccacgtt
     2041 gaatgagacg tcgcgaaccc cgatggtgca ctcggtggct tcacggactt cggcccggga
     2101 agctccaccc tcagcgagct cgatcgcccg ttcgggatga gccccgaaaa ccttccagac
     2161 gtgctcgacc gaaagacata cgtcttccgg cggctcattg gagaaaccca cgtcggtaat
     2221 gtactagcgg gcctcaggcg ggagcctcgg cccacaaccc taggagaagt atgaaaaaga
     2281 gaatttggct acttgt
//
LOCUS       DBNHXU010000098         1692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2807795, whole genome shotgun sequence.
ACCESSION   DBNHXU010000098 DBNHXU010000000
VERSION     DBNHXU010000098.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2807795"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1692
                     /locus_tag="AC161X_02605"
     CDS             <1..>1692
                     /locus_tag="AC161X_02605"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR4988472.1"
                     /translation="GLYGLTRVMSRGEVMGRVEVAGTPIGGMDPDQALTAMVAVEDVY
                     VRRPAIFNLEGKVVSVLPPEAGLEIDEDTIVAEAMKVGREGNQISQFFWWLGHIFSTV
                     DVPVSGSTDDASVDAIFDDWDGDVIASPANQGGVELVDGELTPIYPAIGKGVDRPAAS
                     AIVESSMLAREAHNLAIPVVTVVPLLTEADVDAALAEAQQLLSASIELSDDGTEVVFT
                     TDELAQAFRSETVVEGTPRIINSFDAEVVDELLDPVREDFEDEPVDAEFEISGDSISI
                     VPGAKGTKIDPTETAMRLHAAGLTSERTGELPLVEGADPDVTTEYLEGLNIQHLVSQF
                     TTYHDCCQPRVTNIHQMAETIDGTIVLSGKTFSLNDTVGQRTLEGGYVEAPGILGGVL
                     EESIGGGVSQLTTTLYNAVFWGGYQDVEHKPHSYYFTRYPEGIEATLSWTSPDLKFRN
                     NTDDAVLIDTRYTDTSITVRIFGSNGGRVLKGEQSGGSTHLWTDNEGGPDALHVRGEV
                     SERYALTDPPPTRYVANPDLGVDEQDETQTEEGGWSVTVTREISRGDDVIDSQEW"
BASE COUNT          338 a          537 c          533 g          284 t
ORIGIN      
        1 ttggtctcta cggcctcacc cgggtcatgt cgagaggcga ggtgatgggt cgcgtcgaag
       61 tcgccgggac accgatcggg ggcatggacc ccgaccaggc actgactgcc atggtcgccg
      121 tcgaggacgt ctacgtccgc cggccggcga tctttaacct cgaagggaaa gtggtcagtg
      181 tcctcccccc cgaagccggg ctcgagatcg acgaggacac catcgtggcc gaggccatga
      241 aggtgggccg cgaggggaat cagatctcac aattcttctg gtggctcggg catatcttct
      301 ccacggtcga cgtgccggtg tccggatcta ccgatgacgc atcggtggac gcgatctttg
      361 acgactggga cggcgatgtg atcgccagcc cggcgaatca gggtggggtc gaactagtcg
      421 acggtgagtt gaccccgatc taccccgcaa tcggcaaggg cgtcgaccgg ccggccgcct
      481 cggccatcgt ggaatcgtcg atgctcgccc gtgaggccca caacctggct atcccggtgg
      541 tcaccgttgt tccgttgctg accgaggctg acgtcgatgc cgccctggcc gaggcccaac
      601 aacttctgtc ggcctcgatt gaattgagcg acgatgggac cgaagtggtg ttcaccacgg
      661 atgagcttgc ccaggcattt cgatccgaga ccgttgtcga agggacaccc cgaatcatca
      721 actccttcga cgccgaagtg gttgacgaac tgctggatcc agtccgcgag gacttcgaag
      781 acgagcctgt cgatgccgag ttcgagatct caggtgactc gatcagcatc gtgcccggtg
      841 cgaaggggac gaagatcgac cccactgaga ccgccatgcg gctgcatgcg gccgggctga
      901 ccagtgagcg caccggtgag ctgccattgg tcgaaggcgc tgatcccgac gtcaccaccg
      961 aatacctcga gggcctcaat atccagcatc tggtctcaca gttcaccacc tatcacgact
     1021 gctgccagcc gagagtcacc aatatccacc aaatggccga gaccatcgac gggaccatcg
     1081 tgctctccgg caagaccttc tcactcaacg acacggtcgg gcagcggact ctcgaaggcg
     1141 gctatgtcga ggcgcccggg atcctcggcg gggtgctcga ggagtcgatc ggcggcgggg
     1201 tgagccagct cacaacgacg ctgtacaacg ctgtcttctg gggcggatat caggacgtcg
     1261 agcacaagcc tcactcctat tacttcaccc gctatcccga gggcatcgaa gccaccctca
     1321 gttggacgag ccccgacctc aagttccgca acaacacgga cgatgcggtc ctcatcgaca
     1381 ccaggtacac cgacacttcg atcacggtgc gaatcttcgg cagtaacggc ggacgggtgc
     1441 tcaagggcga gcaaagcggc ggatccaccc acctctggac cgacaacgag ggcgggcccg
     1501 atgcccttca cgtgcggggc gaggtctcgg agcgctacgc cctcaccgat ccgccgccca
     1561 cccgatatgt ggcgaacccc gatctcggcg tggacgagca ggacgagacc cagaccgagg
     1621 aaggcggatg gagcgtgacg gtcacccgtg agatcagccg cggtgacgac gtgatcgaca
     1681 gccaggaatg gg
//
LOCUS       DBNHXU010000099         2818 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1221044, whole genome shotgun sequence.
ACCESSION   DBNHXU010000099 DBNHXU010000000
VERSION     DBNHXU010000099.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2818)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2818)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2818
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1221044"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..248
                     /locus_tag="AC161X_02610"
     CDS             <1..248
                     /locus_tag="AC161X_02610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988473.1"
                     /translation="AWALASCSAEMVVVVLPWVVAVVEVSPDDPSVVVVVLPWLVAVV
                     DVSPDGSPASAHAEMTMHADQVIKARRTQKPFVEYRS"
     gene            308..460
                     /locus_tag="AC161X_02615"
                     /pseudo
     CDS             308..460
                     /locus_tag="AC161X_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015096812.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding domain-containing protein"
     gene            complement(465..1229)
                     /gene="heR"
                     /locus_tag="AC161X_02620"
     CDS             complement(465..1229)
                     /gene="heR"
                     /locus_tag="AC161X_02620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017955883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heliorhodopsin HeR"
                     /protein_id="HIR4988474.1"
                     /translation="MTTVTSIPAARLRGYNLGMGALHAIQGVIVLILANDFTLPVVAT
                     YMTGIPGAADPSIEPLFAVSIAWGVAIFLFMSAVAHLTISLPGVFPWYLRNLESQRNY
                     ARWIEYSFSSSVMVVLIAMLVGIADVAALIAIAGVNASMILFGLLQERYETPGESVSW
                     LSYWFGVLAGAIPWIAIAIYLFSPGFEASPPGFVYWIFFSMFLLFNVFAIVMVLQYKK
                     VGRWSNYVFGESTYILLSLVAKSLLAWLVFGNTLAV"
     gene            complement(1284..>2818)
                     /locus_tag="AC161X_02625"
     CDS             complement(1284..>2818)
                     /locus_tag="AC161X_02625"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR4988475.1"
                     /translation="GLYTTVAALLAYAVFGTNRVLVLGPDSSLAPIIAAIVLPMAAGD
                     PATAVVLASALSLITGLICLVAGYARLGVVTELLSKPVRIGYLNGIAVVVLISQLPKA
                     LGFSVDADTTLSTLVATFEAVVDGQIVFAAMVFSILTITLIQVARTLWPGAPGVLVAV
                     IGSLAGMALFDLSGVVETVGAMPQGAPAFVFPGVDVNDLRALIIGGLGVALISFADTG
                     ALSSAMALRAGGRSKPNSEAKALGAANILSGLLQGFPTSASSSRTAVAVSVGSKTQLT
                     GVVAAVGVLAMLVWAPGLLANLPAATLAAIVIAASFIIFDGAAVRWLWSVRRSEFLLS
                     LGSFLGVVLIGVLEGILIAVVLSLANFIRRAWHPHSAELVRVNGLKGYHDRERHPEGE
                     TIPRLLILRFDAPLFFANAPTFGRLLHDLLEGASRPIDRVLVAGAAITDIDTTGAEVL
                     VGVLDDLDRRGLSFAFAGLKGPVKDRLKAYGVYDRIGEVNFFPTIGEAVNAHEVGPET
                     RG"
BASE COUNT          609 a          866 c          893 g          450 t
ORIGIN      
        1 ccgcgtgggc cttggcgagc tgctcggctg agatggtcgt ggtcgtactg ccctgggtcg
       61 tcgctgtggt cgaggtctcg ccggacgatc cttcggtggt cgtggtcgta ctgccctggc
      121 tcgtagctgt ggtcgacgtc tcaccggatg gttctccagc atctgcacat gccgagatga
      181 ccatgcacgc ggaccaagtc attaaggcca gacgcaccca aaagcctttc gtcgaatacc
      241 ggtcttgacc ataggctggt cggctacgcc atcaaggcga atacggtccc ggcagttgat
      301 gcctcggatt atcgacgccg ggatgcgtca gctggccgcc accggctgga tccagaatcg
      361 ggctcgaatg ctggtcgctt ctttccttgt gaaagacctg ctggtcgact ggcggatcgg
      421 ggcgaggcat ctgcgtaggc atttgctgga tggcgacatc ccgctcaaac tgcgagcgtg
      481 ttgccaaata ccagccacgc gaggagggac ttggcgacga ggctgagcag gatgtaggtc
      541 gattcgccga acacgtagtt cgaccacctg ccgaccttct tgtactgaag caccatgacg
      601 atggcgaaga cgttgaagag caagaacatg gagaagaaga tccagtacac gaagccgggt
      661 ggtgacgcct cgaagcccgg gctgaacaga tatatcgcga tggcaatcca gggaatcgct
      721 ccggcgagca ccccgaacca gtaggagagc cagctaaccg attccccggg cgtctcgtac
      781 ctctcctgga gaagcccgaa caggatcatc gaggcgttga caccggcaat ggcgatcaag
      841 gctgcgacgt cggcgatccc taccagcatc gcgatcagca ccaccatcac cgaggatgag
      901 aatgagtatt cgatccatct ggcgtagttc ctctggctct cgagattcct cagataccaa
      961 ggaaacactc cgggcagact gatcgtgagg tgagcgacgg cggacatgaa caggaagatc
     1021 gcgacccccc aggcgatgct cacagcgaag agcggctcga tcgacggatc ggcggcgccc
     1081 gggatgccgg tcatgtaggt cgccacgacc gggagagtga agtcgttggc aaggatgagc
     1141 actatcacgc cctgaatggc gtggagcgct cccatgccca ggttgtagcc acgcagtctg
     1201 gccgcgggaa ttgatgtcac ggttgtcata tccccccatt cgtacccctc tccctggacg
     1261 gatgacaggg caaaaggccc ttttcagccc ctcgtttccg gtcctacctc gtgagcgttg
     1321 accgcctcgc cgatcgtggg aaagaagttc acctccccga tcctgtcgta gacgccgtaa
     1381 gccttgagac ggtccttcac cgggcccttg agcccggcaa acgcgaacga caggcctcgc
     1441 cgatcgagat cgtcgagcac cccgaccagc acttcggccc cggtggtgtc gatatcggtg
     1501 atggcagcac cggccaccag gacacggtca attggccgcg acgccccctc cagcagatcg
     1561 tggagcaggc gaccaaaggt aggcgcgttg gcgaaaaaga gcggtgcgtc gaatcgaagg
     1621 atcaacaacc gcggaatcgt ctctccctcg ggatgacgct cccgatcgtg gtaccccttc
     1681 agtccgttca cccggaccaa ctcggcgctg tggggatgcc aggcccgtct tatgaaattg
     1741 gccagggaga ggacaacggc gatcaggatc ccctccagca ccccgatgag gaccacccct
     1801 aggaaggagc cgagcgagag caggaactca gagcgccgga ccgaccacag ccagcggacc
     1861 gcggcgccgt cgaagatgat gaaggaggca gcgatgacaa tggcagcaag ggtcgccgca
     1921 ggcagattgg ctagcagccc aggcgcccac acgagcattg cgagcacgcc aacggcagcc
     1981 accaccccgg tgagctgcgt tttcgagccg accgagaccg caacggcagt cctcgatgag
     2041 ctggcactgg tcgggaaccc ttggagcagc ccggagagga tgttggccgc tccaagcgct
     2101 ttcgcctccg agttcggttt cgagcgcccg ccggccctca acgccatcgc actggagagg
     2161 gcgcccgtat cggcaaagga gatcaacgcc acaccgagcc cgccgatgat gagtgcccgc
     2221 agatcgttga catccactcc gggaaacacg aatgccggtg caccctgggg catggccccg
     2281 accgtttcga ccactccaga aaggtcgaac agcgccatgc cggcgagcga cccaatcaca
     2341 gccacgagga cccctggagc ccctggccag agcgtcctcg caacctggat cagggtgatg
     2401 gtgagaatcg agaacaccat ggcagcgaag acgatctggc cgtcaactac tgcctcgaag
     2461 gtggcgacca aggttgaaag cgtggtgtcg gcgtctacgg agaagcccaa tgccttgggt
     2521 agctgggata tcagaacgac aaccgcgatc ccgttgaggt agccgatccg aaccggcttg
     2581 gagagaagct cggtaacgac ccccaaccgg gcgtaaccgg cgaccaggca aatcagcccg
     2641 gtgatgaggc tgagagcgga ggcgagaacc accgcagtgg cggggtcgcc cgcagccatc
     2701 ggaaggacga tggcggcgat gatcggagcg agggacgaat ccgggccgag aacgagcacg
     2761 cggttggtcc cgaacacggc gtaggcgagg agcgcagcca ccgttgtata caggccgg
//
LOCUS       DBNHXU010000100         3043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3800912, whole genome shotgun sequence.
ACCESSION   DBNHXU010000100 DBNHXU010000000
VERSION     DBNHXU010000100.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3043
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3800912"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..351
                     /locus_tag="AC161X_02630"
     CDS             <1..351
                     /locus_tag="AC161X_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent decarboxylase
                     family protein"
                     /protein_id="HIR4988476.1"
                     /translation="FRGLQVWLPLQLHGVAPFRDALDQCLDLAELAYNRLSVIDGIDT
                     PWQPDLSIVAFRFDDDDRGREAMQAINQDRVVHVSPTIVDGRFVLRFAVLNRRTTLDH
                     IDHAIDIIEKTLIG"
     gene            complement(376..1638)
                     /locus_tag="AC161X_02635"
     CDS             complement(376..1638)
                     /locus_tag="AC161X_02635"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR4988477.1"
                     /translation="MAINILHQDLGEAHADLASHDTFLRCVPHATFARMRREEPVAWV
                     EEADGSGFWAVTKHADVEMVSRDFARFTASRGIRIEDMAPDELEARRTMMEFDPPEHA
                     RLRRLVQPGFTPKVVATYEAAFRQLSAQVLDQVVSLREFDFVTEISRQLPIRLLCRLL
                     GVPESDASQLVAWGDQMISNADPEYTPVVIDKDDSDEYRLLPFRSPAAIEVFRYAEEM
                     ALERRAHPEDDIISTLLHAEPDGEPLTDLEFKNFFTLMMVAGNETTRHTISHGLIFLT
                     NHPEQLEDLREDPERLSASATEEILRTSAVTMHFRRTATEDTELGGSSIHQGDRVVVW
                     YPSANYDEEVFDDPYAFDIHRAPNDHLTFGAGRHVCLGASLARLEVRVVFEELLKRVS
                     SIEIVGDPDRLRSNFINGIKHLPVRMSA"
     gene            1695..2549
                     /locus_tag="AC161X_02640"
     CDS             1695..2549
                     /locus_tag="AC161X_02640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010504562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR4988478.1"
                     /translation="MTERRRILVEGQPLEVQRIGGALIADDGRSFVSATATHLPPIEP
                     SKILAVHLNFRSRVEEFMTRLPPAPTYFQKPTTALNGHLGHVVRPVGCRYLNFEGEIA
                     IVIGKTCKDVSPDQAEEYIAGYTIANDFGLHDFRDTDAGSMLRVKGSDTLCPLGPGLV
                     DDWDFRDKAIRTLVNDEVRQEASTSEMAWDMAYLVADLARTITLLPGDVILSGTPAGS
                     RPVEPGDAVTVEVEGLGALTNTIVEGLPVRPDVGAQPSESEEVVSTAFGGDWEFRGIR
                     APQSPEAK"
     gene            2562..>3043
                     /locus_tag="AC161X_02645"
     CDS             2562..>3043
                     /locus_tag="AC161X_02645"
                     /inference="COORDINATES: protein motif:HMM:NF019614.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HIR4988479.1"
                     /translation="MVALLRGINVGGRARLPMTELRSTLVDLGLRDVETYLQSGNVVL
                     ESGDRAVTGLAGRIELAIAETFGLQIRVILREREDLVAVARSHPFLDTEADPSRIHVV
                     FLEVVPEADKVAALDPNRSPSDQFEVIGREIYLRYPNGQGRSKLSLDYFERVLGIAGT
                     A"
BASE COUNT          595 a          972 c          934 g          542 t
ORIGIN      
        1 tttcgaggtc tccaggtgtg gctgccgctg caattgcacg gggtcgcacc gtttcgagac
       61 gccctggatc aatgcctcga cctcgccgag cttgcctaca accgactcag cgtcatcgac
      121 gggatcgaca cgccctggca gccagatctg tcaattgttg ccttccgatt cgacgacgac
      181 gaccggggac gggaggcaat gcaggcgatc aaccaggacc gggttgtcca cgtctcaccg
      241 accatcgtcg acggacggtt cgtgctccgt ttcgccgtcc tcaatcggcg aaccacgctg
      301 gaccacatcg atcacgctat cgacatcatc gagaagaccc tgatcggctg atcagcctcg
      361 ggtcatgaga ccgctctaag cgctcatccg gaccggcaag tgcttgatcc cgttgatgaa
      421 attggaccga agccggtcag gatcgccgac gatctcgatg ctgctgaccc gcttcaacaa
      481 ctcttcgaat accacccgaa cctcgagtcg ggccagggaa gcccccaggc agacgtgtcg
      541 ccccgcccca aaagtgaggt ggtcgttggg agcgcgatgg atgtcaaagg cgtacgggtc
      601 gtcgaacacc tcctcgtcgt aatttgccga cggataccaa acaacgaccc gatcaccctg
      661 gtgaattgaa gacccaccga gctccgtgtc ctcggtggcg gtacgtcgaa aatgcatggt
      721 caccgccgaa gtgcgcaaga tctcctcggt tgccgaggcc gacagcctct cgggatcttc
      781 tcgcagatcc tccagttgct ccggatggtt ggtcaggaag atgagcccat ggctgatggt
      841 gtgcctggtg gtctcgttgc ccgccaccat catcaacgtg aagaagttct tgaactcgag
      901 atcggttagg ggctccccgt ccggctcggc gtggagcagg gtgctgatga tgtcgtcttc
      961 ggggtgggcc ctcctctcga gcgccatctc ctcggcgtaa cgaaacactt cgatcgccgc
     1021 cggtgaccga aacgggagca gccgatactc gtcgctgtcg tccttgtcga tcaccacggg
     1081 ggtgtactcg ggatcggcat tggagatcat ttgatcgccc caggccacca gttgggaagc
     1141 gtccgactcc gggactccga gcaagcgaca caggagccgt atcgggagct gacgcgagat
     1201 ctcggtgacg aagtcgaact cgcgcaatga cacgacctga tcgagaacct gagccgaaag
     1261 ctgtcggaag gccgcctcgt aggtggcgac caccttcggc gtgaacccag gctggacgag
     1321 acggcggagc cgggcatgct ccggggggtc gaattccatc atggtccgtc tcgcctctag
     1381 ctcgtccggc gccatgtcct cgatccggat cccgcgggac gccgtgaacc gcgcgaagtc
     1441 gcggctgacc atctccacat cggcgtgctt ggtcaccgcc cagaacccgg acccgtcggc
     1501 ctcttccacc caggccaccg gctcctcccg gcgcatccgg gcaaaagtgg catggggaac
     1561 gcatcgcagg aacgtgtcgt gagaagcgag gtcggcgtgg gcttccccga ggtcctgatg
     1621 aaggatgttg attgccatga gatcgttagg aaccaaacat aatccggacc gacctctggt
     1681 taccgtgtga accgatgacg gagagacgga ggatcctggt cgaaggccaa cccctggaag
     1741 tccaacgcat cggaggggca ctgattgccg atgacgggcg aagcttcgtt tcggccacag
     1801 caacccatct cccaccaatc gagccatcga agatcctcgc cgtccatctc aacttccgga
     1861 gccgggtcga ggagttcatg acacggttgc ccccggcccc gacctacttc caaaagccga
     1921 ccaccgccct caacggccat ctcggccatg tggtccgccc agtcggatgc cgctacctca
     1981 atttcgaggg cgagatcgca atcgtcatcg gaaagacctg caaggacgtc tcccccgatc
     2041 aggccgagga gtacatcgcc gggtacacca tcgcgaacga cttcgggctt cacgacttcc
     2101 gggacaccga cgccgggtcg atgctccgag tcaaaggatc agacacgctc tgccccttgg
     2161 ggcccggtct cgtcgacgat tgggacttcc gggacaaggc catccgcact ctggtcaacg
     2221 acgaggtgag acaggaggcg agcacctccg agatggcatg ggacatggcg tatctcgtag
     2281 ccgacctggc gcggacgatc accctgctac ccggcgacgt gatcctgagt ggcacgccag
     2341 ccggatcgag gccggtcgag ccgggagacg cggtaaccgt cgaggtcgaa gggttgggcg
     2401 cattgaccaa caccatcgtg gaaggcctcc ctgtgcggcc cgacgtcggc gcccagccga
     2461 gcgagtccga ggaggtggtc tccaccgcgt tcgggggcga ttgggagttc cggggaatcc
     2521 gcgctcccca gagcccggag gccaaatagc ccttgagcct ggtggtcgct ctgctccgag
     2581 gcatcaacgt cggcggtcgg gcccggttgc cgatgaccga gttgagatcc acgctggtag
     2641 atctcggact gcgggatgtt gagacctatc tgcagagcgg caacgtcgtt ctcgaatccg
     2701 gggatcgagc cgtgaccgga ttggctggca ggatcgaact cgcaatcgcc gagaccttcg
     2761 gactccaaat acgggtcatc ctcagggagc gcgaggacct tgtcgccgtg gcccgatctc
     2821 atccgttcct cgacacggag gctgatccct cccggatcca cgtcgtattc ctcgaggtag
     2881 tgccggaagc cgacaaagtc gctgccctcg accccaaccg gtccccaagc gaccaattcg
     2941 aggtgatcgg gcgtgagatc taccttcgat atcccaacgg ccagggccgg agcaagctga
     3001 gcttggacta cttcgagcgc gtgctcggca tcgcaggaac ggc
//
LOCUS       DBNHXU010000101         6597 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4595364, whole genome shotgun sequence.
ACCESSION   DBNHXU010000101 DBNHXU010000000
VERSION     DBNHXU010000101.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6597)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6597
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4595364"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="AC161X_02650"
     CDS             complement(<1..439)
                     /locus_tag="AC161X_02650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013074216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HIR4988480.1"
                     /translation="MAERFSFGKIPEVLPLPDLLAVQHESFRWFLDEGFREIFEEISP
                     IEDFTGSLALELTDHRFGEPQMSLEDAKERDSNYAQPLFVTARFMNRSTGEIKEQQVF
                     LGDFPIMTEKGVFIINGTERVIVSQLVRSPGIYFDVTADKTSDK"
     gene            690..2372
                     /locus_tag="AC161X_02655"
     CDS             690..2372
                     /locus_tag="AC161X_02655"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR4988481.1"
                     /translation="MAKHSSPRSAVVWVTIVVALAAFAAIGLGWSEHVAAAGTVVATN
                     VRYAGMPVEGIPADDVEPLVADRAIDLLSHNLTLVVADREVSVPLGELGFSYDSDDTT
                     KAIVDARHSGQPWDQFAAWVAGPLVEHSVGESWSFDPELATATLASLQALRPEPAVEP
                     RLTSEGSESVDVVPGQMGSVVDIDSLVRALARMNLLDPPQQLEAELIEEPPAISDEKA
                     QSTADELNELTRAGALVSIHGHSRLISAKELRDRLIVTANDEGFEPAFNQVSLQTLLE
                     DKFPQPVGEFKDPVFDVIDDQVRVLTPGVPYQVCCNADSAHQVAQGVLGGSAGPFELT
                     AKTADDPVTRAWASGSMIVEPVGEFTTKHPCCESRVTNIHRIADLVRGVYLLPGETFS
                     INEFVGPRTVAGGFVYAGAIRQGHLVEEVGGGVSQFVTTTFNAAYFAGLDFDEYRSHT
                     IYFSRYPYGREATLGIPHPDLIIRNTTDYPILIWDSYSGTTLTVTVYSTKHIEVEELG
                     QRTSKAGACTHVETDRQRTYPDGKVVVDTVVADYRPGEGLDCSGQPTPEPTG"
     gene            complement(2389..2592)
                     /locus_tag="AC161X_02660"
     CDS             complement(2389..2592)
                     /locus_tag="AC161X_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988482.1"
                     /translation="MITFRIRQATMRAVEQDKSGGRHLKRRLPPWLLGLLLAVVVFGV
                     ALLVANMLGLGDDPVVGGLISFR"
     gene            complement(2592..2951)
                     /locus_tag="AC161X_02665"
     CDS             complement(2592..2951)
                     /locus_tag="AC161X_02665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988483.1"
                     /translation="MIVALGACDSHVPADSSATITGRLVAGPTCPVETNPPDPDCAPR
                     PVPDTEVIATLSDGTELQVVSSLDGTFRIALPPGEVVITFAADEASMVAPDPITATIV
                     ANQTLDLGDLTYDTGIR"
     gene            3057..4328
                     /gene="lysA"
                     /locus_tag="AC161X_02670"
     CDS             3057..4328
                     /gene="lysA"
                     /locus_tag="AC161X_02670"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HIR4988484.1"
                     /translation="MSALSWDLLPDTAGVDEDGHLMIGGCDLVQLAVEHGTPLFVYDE
                     AHIRNRCREAVAAFGAGAVYASKAFLCRAMARLAHDEGMAIDVSTAGELHVCLEAGVP
                     ADRLVFHGNNKSRNELRRALEAGVGRIVVDSFDELDRLQSLHESGRTPPRILLRVTPG
                     VEAHTHDFVSTGQDDSKFGFTVSSGAAALAVVEAMQSTAVDLVGIHAHIGSQVFRVES
                     FAEALGVVAGFARDLDLPELSIGGGLGVAYVEGEVAPSITEWAEAIKGAAAALGVTSN
                     LFAEPGRAIVAQAAITLYTVGTIKDLPGIRTYVAVDGGMSDNPRPILYGSGYETFVPA
                     RVNDDRQAKIRLVGKHCESGDVLVLDGTVPEGLGVGDFIATPVTGAYGHSMGSNYNKI
                     LRPAVLFASGGKARLVVRRETLDDLLLTDLG"
     gene            4363..5526
                     /locus_tag="AC161X_02675"
     CDS             4363..5526
                     /locus_tag="AC161X_02675"
                     /inference="COORDINATES: protein motif:HMM:NF019522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HIR4988485.1"
                     /translation="MPNEPSDFERLTDPDRRRVPRPHGLAGLVALIAVGVVVGLVALY
                     PPSTPDIDLSDFGFADDVVPATVTAVEIAECPDFPGVDCSIVEFSDAEGVVVHTQSFP
                     DDGSAQPQLEVGDEVFLSVVDAGDGTNLYNYADRDRTLLLGAVALLFAAAVVGLGRMR
                     GLAALLGLGISLLVLLGFIVPAIIAGEDAVLVAAVGGSAIILVALYLAHGYTPLTHVA
                     AVGAFAALALTTALSWGVLELAQFTGLAGEESYYLLLIPGFDISGLLLAGIVLGAIGA
                     LDDVTVTQASAVWEVRQANPALESGDLFASGLRVGRDHIASTVNTLLLAYAGAAMPLL
                     ILLSLSRQPFASIASSEVVAVEIVRTLVGSIGLVASVPITTWLASRQAIGSLA"
     gene            5523..5972
                     /locus_tag="AC161X_02680"
     CDS             5523..5972
                     /locus_tag="AC161X_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018162428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc protease family protein"
                     /protein_id="HIR4988486.1"
                     /translation="MKRPVIGWREWVQLPDLGVFVVKAKVDTGAATSSLHAFQLERFE
                     KDGFPYVRFEVHPRQRSKRPSIECEAVVVKETAVRNPGGRTELRPVIASTLVIAGQQI
                     EALINLTGRDEMGYRMLLGRRTLRNRFVVDPGKSYLGPRPVRNEETE"
     gene            5969..>6597
                     /locus_tag="AC161X_02685"
     CDS             5969..>6597
                     /locus_tag="AC161X_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006620359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HIR4988487.1"
                     /translation="MKIAILSRRASLYSTARLREAAIERGHEVSIVDYLRCYMDITAR
                     RPKVLYRGETLNPEAVVPRIGATYTYYGAAVVRQFEMAGIYSVNESQAISRSRDKLRS
                     LQLLSRAGVGLPTTGFAHATQDIGGLLKVAGGPPVVVKLLEGTQGLGVVLAETTKAAE
                     SVIGAFRQLDANILVQEFIKEAGGADIRAFVVGGRVVAAMKRQAPPGEFR"
BASE COUNT         1203 a         2062 c         2081 g         1251 t
ORIGIN      
        1 gcttgtccga ggtcttgtcg gcggtgacgt cgaagtaaat gccaggcgac cggaccagct
       61 gggacacgat gacccgctcg gtcccattga tgatgaacac gcccttctcg gtcatgatgg
      121 ggaagtcgcc gaggaagacc tgctgctcct tgatctcgcc ggtggagcgg ttcatgaacc
      181 gggcggtgac aaacaagggc tgggcgtagt tggagtcgcg ctccttggcg tcctcgagac
      241 tcatctgagg ctcgccgaag cgatgatctg tcagctcgag ggcgagggag ccggtgaaat
      301 cctcgatcgg actgatctct tcgaagatct cgcggaagcc ctcatcgagg aaccagcgga
      361 aggattcatg ttgaactgca agcagatcgg gtagggggag aacttcggga atcttcccga
      421 acgagaagcg ctcagccacg cgcgaagcca agaggtgcct ccttggagat acagattagg
      481 gagagatagg ggtgacgcag acaaggcatg ctaacagacg cgtcagcatg cctccaatcg
      541 gacatcttaa cttttggagc cgggccggtt gaggcctggc tcggacccga gggtcgatag
      601 tcatcatatc gcggggtttc aaaacgcgtc aaggtcttgg gcgaacggtt accaggtaca
      661 gatcgaatcc gccgtaccat cccgccgtct tggccaagca ctcgtctcct cgttcggcgg
      721 tggtctgggt gacgatcgtc gtcgccctcg ccgccttcgc tgccattggg ctcgggtggt
      781 ccgagcacgt cgcagcggcc ggaaccgtgg tggcaacgaa tgtgcgctac gccggcatgc
      841 cggtcgaggg gatccccgcc gatgacgtcg agcccttggt cgccgaccgg gccatcgacc
      901 tcttaagtca caacctgacc ctcgtggtgg ctgatcgaga ggtctcggtc cctctcggcg
      961 agctcggctt cagctacgac agcgacgaca ccaccaaggc catcgtcgat gcccggcaca
     1021 gcgggcagcc ctgggaccag ttcgcagcat gggttgccgg ccccttggtc gaacacagtg
     1081 tcggcgagtc gtggtccttc gacccggagc tggcgacggc gactctcgcc tctttgcagg
     1141 cactcaggcc ggagccggca gtggagcccc gcctcaccag tgaaggaagc gaatcggtag
     1201 acgtggtccc cggtcagatg ggctcggtcg tcgacatcga ctccttggtc cgggcgcttg
     1261 cacgaatgaa cctgctcgac cctccacagc agttggaggc cgagttgata gaagagccgc
     1321 ctgcgatctc ggacgagaag gcccagagca ccgccgatga gctgaacgag ttgaccagag
     1381 ccggcgcctt ggtctcgatc cacggacact cccgactcat aagcgcgaaa gagctacgag
     1441 accgcttgat cgtcaccgca aacgatgagg gattcgagcc ggcgttcaat caggtgagcc
     1501 tccagacgct gctcgaggac aagttcccgc agcctgtcgg agagttcaag gatcccgtct
     1561 tcgatgtcat agacgatcag gtgcgagtgc tgacgcctgg agtgccctat caggtctgct
     1621 gcaacgccga ctcggcccat caagtggcac agggagtgct cggaggatca gccggtccct
     1681 tcgagttgac cgccaagacg gccgatgacc cggtgactag agcctgggcc agtgggtcga
     1741 tgatcgtcga gcccgtcgga gagttcacca ccaagcatcc ctgttgtgag tcgcgagtca
     1801 cgaacatcca ccgcattgcc gacctggtgc gaggggtcta tctcctgccc ggggagacct
     1861 tctcgatcaa tgagttcgtc ggaccgcgca ccgtggctgg agggtttgtg tatgcgggag
     1921 cgatccgcca gggccacttg gtagaagagg tgggaggcgg ggtttcgcaa ttcgtcacca
     1981 ccacattcaa cgcggcctat ttcgctggtc tcgatttcga cgagtatcgg agccacacca
     2041 tttacttttc tcgctacccc tacgggcggg aggcgactct gggcatcccc caccccgacc
     2101 tgattatcag aaacaccacc gactacccga tcctgatctg ggacagctac tccggcacga
     2161 cgctgaccgt gaccgtctac tcgaccaagc acatcgaagt cgaggagttg ggtcagagga
     2221 cgtcgaaggc cggtgcctgc acccatgtcg agaccgatcg acagaggacc taccccgacg
     2281 ggaaagtggt ggtcgacact gtggtcgcgg attatcggcc tggagaaggg ctcgactgca
     2341 gcggtcagcc cactcccgag ccaaccggct gaaatcggcc gtccgagctc acctgaatga
     2401 aatcaggcca cccaccacag ggtcgtcacc gagtccgagc atgtttgcca ccaaaagggc
     2461 gaccccaaag acgaccacag cgagaaggag cccaagcaac caggggggca ggcggcgctt
     2521 caggtgtcgt ccgccggact tgtcttgttc cacggctcgc attgtggcct gacgaattct
     2581 gaacgtgatc atcagcggat tccggtgtcg tatgtcagat cgccgaggtc cagcgtttgg
     2641 ttggcgacga tggttgcggt gattggatcc ggggcaacca tcgaagcctc gtccgcggca
     2701 aaggtgatga cgacttcacc ggggggaagc gcgatccgga acgttccgtc caaactcgag
     2761 acaacctgga gctcggttcc gtcggacaga gtcgcaatga cctcggtatc gggcacaggc
     2821 ctaggcgcgc aatcagggtc gggagggttg gtctcgaccg gacatgtcgg cccggcgacc
     2881 aggcgaccgg tgatggtggc gctgctgtcg gccggaacgt gagaatcgca ggcacccagg
     2941 gcaacgatca tcagagctcc tgcgagaaag catctcatct ctgtttgacg tcccctgccc
     3001 aatctccgtt cccagacccg acgaagcgtg aaccgggcct tcataggatt cccaggatga
     3061 gcgcactctc ctgggacctc ctccccgaca cggcaggagt cgatgaggac ggccacctca
     3121 tgatcggggg gtgtgacctc gtccaactcg ccgttgagca cgggactccc ctctttgtct
     3181 acgacgaggc ccatatccgg aaccgatgcc gggaagccgt ggcggcgttc ggggccggcg
     3241 ccgtctatgc atccaaggct ttcctgtgtc gggccatggc ccgtctcgct cacgatgagg
     3301 gcatggcaat cgacgtctcc acagcaggag agctccatgt ctgtctcgag gcaggtgtgc
     3361 ctgcggatcg cctcgtcttc cacggcaaca acaagtcgcg caatgagttg cgccgggctc
     3421 tcgaggcggg agtcggtcgg atcgtcgtgg acagcttcga tgagttggac cgcctgcaat
     3481 ccctccacga gtcgggtcgg acccctcccc ggatcctgct tcgggtcacc ccgggtgtgg
     3541 aggcccacac ccatgatttc gtttcgaccg gccaggacga ctccaaattc gggttcacgg
     3601 tctcgtccgg agcggcagcc ctggccgttg tcgaggccat gcagtcgacc gcagtcgacc
     3661 tggttgggat ccacgcccac attgggtccc aggtgtttcg ggttgagtcc tttgccgagg
     3721 ctctgggcgt cgtcgccggt ttcgctcgcg accttgacct gccggagttg tcgattggcg
     3781 gtggtctcgg cgtggcatac gtcgagggtg aggtcgcccc gagcatcacc gagtgggcag
     3841 aagcaatcaa gggtgccgcc gccgccctcg gagtcacttc gaatctgttc gctgagccgg
     3901 gacgggcgat cgtggcgcaa gcagcaatca ccctctacac ggtgggcacc atcaaggacc
     3961 tgcccggcat ccgcacttat gtcgcggtcg acggtgggat gagcgacaac ccgcgcccga
     4021 tcctctatgg gtctggctac gagactttcg tgccggccag ggtgaacgat gaccgacagg
     4081 caaagatccg gctggtgggg aaacactgcg agtcaggcga cgtcttggtc ctggacggaa
     4141 ccgttcccga gggattggga gtcggtgatt tcattgccac cccggtgacc ggtgcgtatg
     4201 ggcattcgat gggatccaac tacaacaaga tccttcgacc tgctgtgctg ttcgcttctg
     4261 gaggcaaggc ccgcttggta gttcggcgag agacactcga cgatctgctg ctcaccgatc
     4321 tcggatgatc ctccccacca gcgcctacct acgattctga ccgtgcccaa tgaaccgtcc
     4381 gacttcgaac gtctcacgga cccggatcga cgtcgtgtcc cccggcctca tgggctcgcc
     4441 gggttggtgg cgctgatcgc agttggggtg gtcgtcgggc tcgtggccct ctatccacca
     4501 agtaccccgg acatcgacct cagcgatttc ggcttcgccg acgacgtcgt gccagccacg
     4561 gtcaccgcgg tcgaaatcgc agagtgtccc gacttcccgg gcgtcgactg ttcgatcgtc
     4621 gagttctcgg acgcagaggg agtcgtggtc cacacccagt ccttcccgga tgacggatcg
     4681 gctcagcccc aactcgaagt aggagacgag gtcttccttt cagtggtcga cgccggggat
     4741 gggacgaacc tctataacta tgcggaccgc gaccggacac tccttctcgg cgcggtggct
     4801 ctgcttttcg ccgccgcggt cgtgggcctg ggcaggatgc ggggcctggc tgcacttctc
     4861 ggactcggga tcagccttct cgtcctactc ggcttcatcg tcccggcgat catcgccggg
     4921 gaggacgccg tcctcgttgc cgccgtgggc ggaagcgcca tcatcctcgt ggctctttac
     4981 ctggcacacg gctacacacc tctcactcac gttgcagcgg tcggagcctt cgccgcgctc
     5041 gccttgacca cagccctctc ctggggtgtc ctggaacttg cgcagttcac tggccttgcc
     5101 ggggaggagt cgtactacct gctcttgatt cccggcttcg acatctccgg ccttctgctc
     5161 gctgggatcg ttctcggagc gatcggcgct ctcgacgatg tgaccgttac ccaggcctcc
     5221 gcggtgtggg aggtgcgcca ggccaaccct gcactagaga gtggcgacct cttcgcgtct
     5281 ggcttgaggg tgggccggga tcacatcgct tccacggtca atacgctcct cctcgcctat
     5341 gccggagcag ccatgccact tctgatcttg ttgagcctgt ccaggcagcc atttgcgtcg
     5401 atcgcaagct ctgaagtggt ggcagtcgaa atcgtcagaa ctctcgtggg gtcgattggc
     5461 ctcgttgctt cggtgccgat caccacctgg ttagcgtcgc gacaggcaat tggttccttg
     5521 gcatgaaacg gcctgtgatc ggttggaggg aatgggtcca gttgccagac ctcggcgtgt
     5581 tcgtcgtcaa ggcgaaggtg gacacgggcg ccgccacctc gtcgctccac gcattccaac
     5641 tggagcgctt cgagaaggat ggtttcccat atgtccggtt cgaagttcat ccccgacaac
     5701 gctcgaagcg gccttcgatc gagtgcgagg ctgtggtcgt caaggagacg gccgtccgca
     5761 acccgggtgg tcgcaccgag ctcaggcctg tcatcgcctc gacgctcgtg atcgccggcc
     5821 agcagatcga ggccctgatc aacctgacag gaagagatga gatgggatac cggatgcttc
     5881 tcggccgacg caccctgaga aaccggtttg tggtagaccc cggcaagagt tacctgggcc
     5941 cgcgccccgt acgcaacgag gagaccgagt gaagatcgcc atcctctccc gaagagcaag
     6001 cctctattcg accgcccggc tccgtgaagc cgcaatcgag cgcggccacg aggtcagcat
     6061 cgtcgactac ctccgttgct acatggacat cacggcccgc cgccccaagg tcctctaccg
     6121 cggggagaca ctcaaccctg aagcggtggt cccccgaatc ggcgccacct acacctatta
     6181 cggggcggcg gtggttcgcc agttcgagat ggccggcatc tactcggtca acgagtcaca
     6241 ggcgatcagc cgatcgcgtg acaagctccg gtctcttcag ctgttgtcac gggcgggagt
     6301 gggcctgccc acgaccgggt tcgcccatgc cacccaggac atcggagggc ttctcaaagt
     6361 cgccggcggc ccaccggtgg tggtcaagct cctcgagggc actcagggtc tcggtgtcgt
     6421 gctcgcagag accaccaaag ccgccgaatc cgtgatcggg gccttccggc aactcgacgc
     6481 caacatcctc gtccaggagt tcatcaagga agcgggtggg gccgacatca gggcgttcgt
     6541 agtcggtgga agggtggtcg ccgccatgaa gcgacaggct ccgcccggag agttccg
//
LOCUS       DBNHXU010000102         5992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4199270, whole genome shotgun sequence.
ACCESSION   DBNHXU010000102 DBNHXU010000000
VERSION     DBNHXU010000102.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5992
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4199270"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..659)
                     /gene="trhA"
                     /locus_tag="AC161X_02690"
     CDS             complement(<1..659)
                     /gene="trhA"
                     /locus_tag="AC161X_02690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019835 - cytolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAQR family membrane homeostasis protein TrhA"
                     /protein_id="HIR4988488.1"
                     /translation="MERLTLGRMQNPIRGFLHGTAAIGAVVGLFVLLNRAWGSVAAAV
                     GALVFGLAMLAMYTVSSLYHSVPWSEKWKVRLQRVDHSMIFLLVAGTFTPIAIATIDG
                     AALAIVLCLLWTTALTGIILKVFLPNVKTSLSVTLQLMMGWSALILMPWVLSRLGVGA
                     VVLIALGGLCYTAGAVIFMSKRPKLFPQTFSYHELFHVLVIAGTAFHFLAVYWYAIPA
                     TV"
     gene            755..1525
                     /gene="uppS"
                     /locus_tag="AC161X_02695"
     CDS             755..1525
                     /gene="uppS"
                     /locus_tag="AC161X_02695"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HIR4988489.1"
                     /translation="MALGLLYRIYERNLLNGLDKDRLPRHIGIVLDGHRRHAREEKLP
                     SYTESYRVGMRRFEEFLGWAHELEIPAVTAWVLSKENISSRPEDELEPYFQVLIELFE
                     RLPALCEKYDMAIKFIGSLDLLPDQLVSAAKSAEEAHPRGSRRLNVAMGYGGRQEIVD
                     AAKDLVSELLSDGVPPEDVASYIDSDALAAHMYSADLPDPDLLIRTSGESRLSGFLLW
                     QSAYAEFVFVDVNWPAFRRVDFLRALRDFTSRDRRFGK"
     gene            complement(1541..3076)
                     /locus_tag="AC161X_02700"
     CDS             complement(1541..3076)
                     /locus_tag="AC161X_02700"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012395967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR4988490.1"
                     /translation="MADKPNILVIWGDDIGITNLSCYSDGLMGYRTPNIDRVANEGMR
                     FTDSYAEQSCTAGRSSFITGQSVYRTGLSKVGIPGSPIGLSAEDPTIAELLKPLGYAT
                     GQFGKNHLGDLNKFLPTVHGFDEFYGNLYHLNAEEEPERPNWPPAEDFPRFNEMARPR
                     GVIHSWATDEDDPTEIGRFGPIGKQRIEDTGPLTKERMETIDDETTEACVDFIKRQHE
                     SGTPFFAWMNTTHMHAFTHTKAESLGQAGRWQSPYHDTMIDHDRNVGTLLDVLDELGI
                     AEDTIVIYSTDNGPHMNTWPDGGMTPFRNEKNSNWEGAFRVPEMIRWPGKIEAGTISN
                     EIIQHHDWLPTFLAAAGEPDIMEKLKTGHTIGDKTYKVHIDGFNLLPYLTGEEEKSPR
                     KGFVYFSDDGDVLGLRFDNWKLVFMEQRVQGTLQIWVDPFIPLRVPKLFNLRTDPFER
                     ADITSNTYFQWVFENAYLVFAGTTLITEFLATFAEFPPRQKAASFTIDQALAKMEATL
                     AGG"
     gene            complement(3145..3831)
                     /locus_tag="AC161X_02705"
     CDS             complement(3145..3831)
                     /locus_tag="AC161X_02705"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR4988491.1"
                     /translation="MDLFLVRHGQPDWAPDRVARNDPNLTELGHEQARRVAHRLASLD
                     SFDEVWVSSMNRAVQTSDPIATDLGTRAEIYDWLQEINNPPEWDGSPVDEIDKALRDS
                     NLRSMDEMWDGLPGGETFRTFHQRVVNGLQHTLDHHGIHPMQEEHRHLWAVDDPDRRV
                     LIVAHAGTNAVVLGHLLGLEPVPWEWERFRQPHTGVSRLTMTRISTGWAFSLRQLGDV
                     THLHPGMVTA"
     gene            3905..4450
                     /gene="pyrE"
                     /locus_tag="AC161X_02710"
     CDS             3905..4450
                     /gene="pyrE"
                     /locus_tag="AC161X_02710"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015244681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HIR4988492.1"
                     /translation="MAGESLIQHLQTHALRTDGPFTLRSGAVTSWYLDARQTTFSGEG
                     AWLVGTAVLAVVDSTAQAIGGMTLGADPIAVATAMVAARDGRRLTAFSIRKEAKDHGT
                     GGRVVGPVAPGTRVVILEDTTTTGSAALEATAVAEAEGLDVIQAVALVDRSEGRAAEA
                     FAARDIPYIALVTPSDLGVDE"
     gene            4447..5034
                     /locus_tag="AC161X_02715"
     CDS             4447..5034
                     /locus_tag="AC161X_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988493.1"
                     /translation="MKVVVQRSAARMWLMAIGAIPLLVIAVDVLTNRRITNWLRELLF
                     TPADTQIYEPRDVIYAWGLLLFAGFLVIWGLKELFMPTRVIECRDAGLALKLRGPFRP
                     HDLIPWGNLVDVDAGEIKDEGEILLLLRIKVLERGSLPAHPWGARWLETRVLGILAQD
                     WAVTPKEAAKLIGDYAVDAARRAERARVTGLWEPS"
     gene            5031..5807
                     /locus_tag="AC161X_02720"
     CDS             5031..5807
                     /locus_tag="AC161X_02720"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019874349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HIR4988494.1"
                     /translation="MRAVGAHVDSSRPIEEAQERGADLVQIFLSDPQGWKKPLPRADA
                     PELRASPIPIYVHSPYLINVCADNNKIRVPSRRILQETCDAAAEIAAAGVVVHGGHVT
                     GDGPQEEGILRWRKALEPLDSQVPVLIENTAGGDKAVARRVEWIARLFDEVAEFGVGF
                     VLDTCHVWAGGEPFDELVPRVLEATGRIDLVHANDSKDPFDSRRDRHENLGLGQIPSE
                     DLALVIAAADAPVVVETPGGAAEHTADIEWVRSAVSARSS"
     gene            5854..>5992
                     /locus_tag="AC161X_02725"
     CDS             5854..>5992
                     /locus_tag="AC161X_02725"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF014593.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR4988495.1"
                     /translation="MTKKTTVPDIRARKGGPKITMVTAYDYPSAVIADRAGMDIILVG
                     DS"
BASE COUNT         1063 a         1821 c         1960 g         1148 t
ORIGIN      
        1 accgtcgccg ggatggcgta ccagtacacg gcgaggaaat ggaacgccgt gccggcgatc
       61 acgagcacat ggaatagctc gtggtaggaa aacgtctgag ggaacagctt ggggcgcttc
      121 gacatgaaga tcacggcccc agccgtgtag cagagcccgc cgagggcgat caggacgacc
      181 gccccgacac cgagccggct cagcacccac ggcatcagga tcagggccga ccatcccatc
      241 atcaactgca acgtcaccga tagggaggtc ttcacgttgg ggaggaacac cttgaggatg
      301 atgccggtca gcgcagtcgt ccacagcagg cagaggacaa tcgccagggc agccccgtca
      361 atggttgcga tggcgatcgg ggtgaaagtc cccgcaacga ggaggaagat catcgagtga
      421 tccacccttt gcagacgcac cttccacttt tcgctccatg ggacggagtg atacagggag
      481 gagaccgtgt acatggcgag catcgccaga ccgaatacca gagccccgac ggcagcggcg
      541 acgctgcccc aagcccggtt cagcaacacg aagagcccga ctacggcgcc gatcgccgcg
      601 gtcccgtgga ggaagccacg gatcgggttt tgcatgcggc cgagggtcaa acgctccatg
      661 tagccatcag gctattgggg atcggtgcca actagtaagc caaggcgtca acactggcgg
      721 cgcttctggt cgccggtagc atctagggac cgtcatggcc ctgggtcttc tctaccgcat
      781 atacgagcga aacctgctca acggtctcga caaggaccgt ttgcctcgcc atatcggcat
      841 cgtcctcgac ggtcatcgtc gccatgcccg tgaggagaaa ctcccctcct acaccgagtc
      901 gtatcgggtg gggatgcgtc gtttcgaaga attcctcgga tgggcccacg aattggagat
      961 cccggccgtc accgcctggg ttctctccaa ggagaacatc agcagccgac ccgaagacga
     1021 attggagccc tattttcagg tgctgatcga gctcttcgaa cgactgccgg ccctttgtga
     1081 gaagtacgac atggcgatca agttcatcgg gagcctcgat ctccttcctg atcagctggt
     1141 gtcagccgcc aagagcgccg aggaggctca tccgcgaggg tcgcggcgtc tcaatgtggc
     1201 gatgggatac ggcggtcgcc aggagatcgt ggacgccgcc aaggacctcg tgtccgagct
     1261 tctctccgac ggggtcccgc ccgaggacgt cgcgtcctat atcgactccg acgccctcgc
     1321 cgctcacatg tactcagcgg accttcctga tccggatctg ctgatccgga cttcggggga
     1381 atcccgtctc tccggcttcc tcctctggca gtccgcctac gccgagttcg tcttcgttga
     1441 cgtcaactgg cccgcattcc ggcgcgtcga cttcctgcgg gcattgcgcg acttcaccag
     1501 ccgcgatcgc cgcttcggga agtagcacga cctttcgggc tcagcctccg gccagggtgg
     1561 cttccatttt cgccagggcc tggtcgatgg tgaaggacgc cgccttctgc cgtggcggga
     1621 actcggcgaa cgtcgccagg aactcggtga tcagcgtggt ccccgcaaac accaggtagg
     1681 cattctcgaa aacccactgg aagtaggtat tcgatgtgat gtctgcccgc tcaaaggggt
     1741 cggtgcgcag gttgaacagc ttggggaccc gtagtgggat gaatgggtcg acccatatct
     1801 ggagcgtccc ttgcacccgt tgctccatga acaccagctt ccagttgtcg aagcgaagcc
     1861 cgagcacatc accgtcgtcg gagaaataga cgaagccctt tcgtgggctc ttctcctcct
     1921 caccggtcaa gtatggaagg aggttgaaac catcgatgtg caccttgtag gtcttgtccc
     1981 cgatggtgtg tccggtcttg agcttctcca tgatgtctgg ctcaccggcc gcggccaaga
     2041 aggtcggcag ccagtcgtga tgttggatga tctcgttcga gattgtcccg gcctcgatct
     2101 tccccggcca acggatcatc tcggggaccc ggaacgctcc ttcccaattg gagttcttct
     2161 cattccggaa aggagtcatc ccaccatccg gccaggtgtt catgtgggga ccgttgtcag
     2221 tcgaatagat gacgatggtg tcttcggcga tccccaactc gtcgagtacg tccagcaggg
     2281 tccccacgtt gcggtcgtgg tcgatcatcg tgtcgtgata cggcgactgc caacgcccgg
     2341 cctgcccgag gctctccgcc ttggtgtggg tgaaggcgtg catatgcgtg gtgttcatcc
     2401 aggcgaagaa cggggttccg ctctcgtgct gcctcttgat gaagtcgacg caggcctcgg
     2461 tggtctcgtc gtcgatggtc tccattcgct ccttggtcag gggcccggtg tcctcgatcc
     2521 gttgcttgcc gatcgggccg aaacggccga tctcggtcgg gtcgtcctca tcggtggccc
     2581 aagagtggat gactccacgg ggccgtgcca tctcattgaa ccgggggaag tcctctgccg
     2641 gaggccaatt cggccgctcc ggctcctcct cggcgttgag gtgatagaga ttcccgtaga
     2701 actcgtcgaa accgtggacg gtgggaagga acttgttgag gtcaccgagg tggttctttc
     2761 cgaactgccc tgtggcatag ccgaggggct tgagcagctc ggcaatggtg gggtcctcgg
     2821 cgctgagacc gatcggcgat ccggggattc cgaccttgct caacccggtt cggtacacgc
     2881 tctggccggt gatgaaggag gaccggcctg cggtgcagct ctgctcggcg taggagtcgg
     2941 tgaagcgcat tccttcgttg gcgacgcggt cgatgttggg ggtgcggtag cccatcagcc
     3001 catcgctgta acagctcaga ttggtgatcc cgatgtcgtc accccagatg acgaggatgt
     3061 tcggtttgtc tgccatgggt ggggtccttt ccaagtgggc cgtggaacat acacgttgag
     3121 atcgggaccg ttgtcggccg gcagttatgc agtaaccatt cccgggtgga ggtgggtgac
     3181 gtcgccgagt tggcggagcg agaaggccca tcccgtgctg attcgggtca tggtgagccg
     3241 ggagaccccg gtgtgaggct ggcggaaccg ctcccactcc cacggcaccg gttccaggcc
     3301 gagcaggtgg ccaaggacca ctgcattcgt tcccgcgtgg gccacgatta ggaccctgcg
     3361 atcggggtcg tcgaccgccc agagatgccg gtgctcctcc tgcatcgggt ggatgccatg
     3421 gtggtcgagg gtgtgttgca gaccgttcac cacccgctga tggaaggtgc gaaaggtctc
     3481 accccccggc agcccgtccc acatctcatc catggagcgc aggttggagt cacggagcgc
     3541 cttgtcgatc tcgtcaaccg gggatccgtc ccattccggg ggattgttga tctcctggag
     3601 ccagtcgtag atctcggccc gggtgccaag gtcggtggca atcgggtcgg aagtctggac
     3661 cgcccggttc atcgacgaca cccacacctc atcaaagctg tcgagactgg ccagacgatg
     3721 ggccacccga cgggcctgct catggccgag ctcggtcagg ttcgggtcgt tgcgtgcgac
     3781 acgatccggc gcccaatccg gctggccatg gcggacgagg aatagatcca tgtggtgaaa
     3841 cctagacccg gcgccgacca ccggcgaacg accggtaccg gttttggccc aataccatgc
     3901 gagggtggcc ggcgaatctc taatacagca tctgcagact cacgccctcc gcaccgatgg
     3961 accgttcacc ctgcgttcag gggcggtcac gtcctggtat ctcgatgctc gccagacgac
     4021 gttcagcggt gagggcgcct ggctggtcgg aaccgcagtg ctcgcggtgg tggattcaac
     4081 cgcccaggcg atcgggggga tgactctggg cgccgacccg atcgcagtgg cgaccgcgat
     4141 ggtggctgcc cgtgacggac gccgcctcac cgctttctct attcgcaagg aggccaagga
     4201 ccacggcacc gggggccggg tcgtcggccc ggtggcccct ggtacgcgtg tggtcatcct
     4261 cgaagacacc acaactaccg gcagcgccgc tttggaggcg accgctgtcg cggaggccga
     4321 gggcctcgat gtgattcaag cggtagcgct ggtcgaccgt tcggagggcc gggcggctga
     4381 ggcgttcgcc gccagggaca tcccatacat cgccctggtc accccctccg atctcggggt
     4441 cgacgagtga aggtcgtggt ccagcgctcg gcggctcgaa tgtggttgat ggcgattggc
     4501 gccatacctc tgctggtcat tgcggttgac gtgctgacca accgccgcat cacgaattgg
     4561 ctcagggaat tgctgttcac ccccgcagac acccagatct atgagcctcg cgatgtcatc
     4621 tatgcgtggg ggctgttgtt gttcgcgggg tttctggtga tctgggggct caaggagctg
     4681 ttcatgccca ccagggtgat cgagtgccgt gacgcgggcc tggccttgaa attgcggggc
     4741 ccgttccggc cccacgacct cattccgtgg gggaatctgg tcgacgtcga cgccggcgag
     4801 atcaaggatg agggagagat tctccttctg ttgcggatca aggtcctgga acggggatca
     4861 ttgcctgccc acccctgggg cgcccgttgg ttggagacac gggtgctcgg gatcctcgcc
     4921 caggattggg cggtgactcc gaaggaagcc gccaagctga taggtgacta tgcggtcgac
     4981 gccgcgcgcc gggccgagag agccagggtc acggggctct gggagccatc ttgagggcag
     5041 tcggcgccca cgtggactcg agccggccaa tcgaggaggc ccaggaacgc ggggctgatc
     5101 tggttcaaat cttcctctcc gatccgcagg ggtggaagaa gcccctcccc agggcagatg
     5161 cacccgaatt gagagcatcc ccgatcccga tatatgtgca ctcgccctat ctgatcaacg
     5221 tgtgcgcgga caacaacaag atccgggtgc cctcccgtcg gatcctccag gagacgtgtg
     5281 acgctgccgc cgaaatcgcg gccgccggcg tcgtggtcca cggcgggcat gtcaccggtg
     5341 atggcccgca ggaagagggg atcctgcgtt ggcgcaaggc cctggagccc ctggacagcc
     5401 aggtgccggt gctgatcgag aacacggctg gtggggacaa ggccgtggcc aggcgagtgg
     5461 aatggattgc ccgccttttc gacgaggtgg ccgaattcgg cgtgggcttc gtcctcgaca
     5521 cctgccacgt ctgggcggga ggggagccct ttgacgagtt ggttccgaga gtgttggagg
     5581 cgaccggtcg aatcgacctg gtgcacgcca acgactcaaa agacccgttc gactcccggc
     5641 gggaccgcca cgagaacctg ggtttgggcc agatcccatc tgaggacctc gcactggtga
     5701 tcgcggcggc cgatgctccc gtggttgtgg agactcccgg cggggcggcg gagcacactg
     5761 ccgacatcga gtgggtgcga tcggcggtca gcgcacggtc ttcctgattg accgtccagg
     5821 taccgattac cgagtagcaa gtacccttta tccatgacca agaagacgac ggttcctgac
     5881 atccgggccc ggaagggtgg gcccaagatc acgatggtga ccgcctacga ctacccgtcc
     5941 gccgtgatcg cggatcgtgc cgggatggac atcatcctgg ttggtgattc gg
//
LOCUS       DBNHXU010000103         2375 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4398600, whole genome shotgun sequence.
ACCESSION   DBNHXU010000103 DBNHXU010000000
VERSION     DBNHXU010000103.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2375)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2375
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4398600"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="AC161X_02730"
     CDS             <1..270
                     /locus_tag="AC161X_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007089322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR4988496.1"
                     /translation="QKEAVLAAKSSIVTVEEIVEELEPRAPNWVSLPGWAVSVVVLAP
                     GGSHPSYAMGYSTRDNAFYKAWDSISRDRDQFMAWMTANVLESGT"
     gene            272..1039
                     /locus_tag="AC161X_02735"
     CDS             272..1039
                     /locus_tag="AC161X_02735"
                     /EC_number="2.8.3.-"
                     /EC_number="4.1.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010627498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-transferase subunit beta"
                     /protein_id="HIR4988497.1"
                     /translation="MTGFGYSTDEMMSVTAARYLKNGQVCFVGIGLPSQAANLARLSH
                     APEIVLIYESGTIATRPDVLPLSIGDGELAETAELVVSVPEIFAYWLQAGRIDVGFLS
                     AAQIDRYANLNSTVIGDYDHPNVRLPGAGGAPEIASGAGEVYVMLRQSTKTFVESVDF
                     VTSLGFGRRGEGRPRGAGGGPKVVITDLGVLEPDPSTHELTLVATHPGVEVEDVLGQT
                     GWDLAVADQVASTDPPSHQELAILRDLVARTEAAHRG"
     gene            complement(1054..>2375)
                     /locus_tag="AC161X_02740"
     CDS             complement(1054..>2375)
                     /locus_tag="AC161X_02740"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4988498.1"
                     /translation="LGSDRIAYEVFGESPDSSIDLVITAGSFGAFDSDWEDPQAELFF
                     RRLASFARVIRFDRRGTGASDPLPLDALPPWESFVEELECVMEEVGSQRAALMTTYDA
                     GPMGMLFAATKPERTAALILVNTSSKYVASADYPIGLPPEAADELNRNVADRWGSGDH
                     VLLQVPSRANDERFKAWYGKKTRSIAGPNAAAAYFRAMFSADARSLLPAIHVPTLVLH
                     RKAYRFMPIAHGQYLADNIEGARMVELPGSDGPLYWDHPDEALNAIEEFLTGVTPTSP
                     IDRVLATVLYTDIVDSTRLLAEMGDARWRDILDTHDDLAGRLAFENGGRLIKSTGDGI
                     LATFDGPGRGIRFATGFRQQLHTIAITIRSGLHTGEVEIRGNDVGGMAVHLAARIMGE
                     AGDGEILVSRTVKDLVVGSDLTFEDRGVHHLKGIDGDWPLLAVVPPG"
BASE COUNT          407 a          743 c          792 g          433 t
ORIGIN      
        1 cagaaggagg cggtgctcgc cgccaagagc tcgattgtga ccgtcgagga gatcgtggag
       61 gagctcgagc cacgcgctcc gaattgggtg tccctccccg gttgggcggt gtcggtcgtg
      121 gtgcttgccc ccggcggctc gcacccttcc tacgcgatgg gctactccac cagggacaat
      181 gccttctaca aggcgtggga cagcatctcc cgcgatcgcg atcagttcat ggcctggatg
      241 accgcgaatg tcctcgagtc gggtacctga gatgacaggt ttcggttaca gcaccgacga
      301 gatgatgtcg gtcaccgccg ctcgctacct caagaacggg caggtctgtt tcgtggggat
      361 cgggcttccg agtcaggcgg ccaatctcgc ccggctctca catgcccccg agatcgttct
      421 catctacgag tcagggacca tcgcgacccg acccgacgtg ttgcccctct cgatcggcga
      481 cggtgagttg gcagagaccg ctgagctggt ggtctcggtc cccgagatct tcgcctactg
      541 gcttcaggcc ggtcggatcg atgttgggtt tctgtcggca gcacagatcg accggtacgc
      601 gaacctcaac agcactgtga tcggcgacta cgaccatcct aatgtgcgcc tgccgggtgc
      661 cggtggtgca cctgagatag cgtccggcgc cggcgaggtc tacgtcatgc tccgtcagtc
      721 gacgaagaca tttgtggagt cggtggactt tgtcacgagt ctcggtttcg gaaggcgcgg
      781 agaaggtcgt ccccgcggag ccggtggcgg ccccaaggtg gtcatcaccg acctcggggt
      841 gctcgagcct gatccctcga cccacgaatt gaccctggtg gccactcatc ccggggtcga
      901 ggtcgaagat gtgcttggtc agaccgggtg ggacctggca gtcgctgacc aggtggccag
      961 cactgaccca cccagccacc aggagctggc gatcctgcgc gatctggtgg cgcgaaccga
     1021 ggcagctcac agaggctgag cccgccggcc tatctagccg ggagggacca cggccagcaa
     1081 cggccagtcg ccgtcgatgc ccttgagatg gtggactccg cggtcctcga aagtcaggtc
     1141 ggagccaacg accagatcct tcaccgtccg ggagaccagg atctcgccgt cgccggcctc
     1201 acccatgatg cgagcggcga ggtggaccgc catccccccg acgtcattgc cccgaatctc
     1261 gacctccccg gtgtggagcc cggaccggat cgtgatcgcg atggtgtgaa gctgctgtcg
     1321 gaatcccgtt gcgaagcgga tgccccttcc tggtccatcg aaggtggcca ggatgccgtc
     1381 gccggtgctc ttgatcagcc tgcctccatt ttcgaaggcg agacgcccgg ccaggtcatc
     1441 atgggtgtca aggatgtcgc gccagcgggc gtcgcccatc tcagcgagta gacgtgtcga
     1501 gtcgacgatg tccgtgtaga ggaccgtggc caggacccgg tcgatcgggc tggtcggggt
     1561 gactcctgtc agaaactcct cgatggcgtt gagcgcctcg tcggggtggt cccagtagag
     1621 ggggccatca gagccgggga gctccaccat ccgggcccct tcgatgttgt ccgccagata
     1681 ctgcccgtgt gcgatcggca tgaatcggta agccttgcgg tgaagcacca gagtcgggac
     1741 gtgaatcgca gggagcaggg accgggcatc tgcgctgaac atggcccgga agtaggcagc
     1801 ggcggcattg gggccggcaa tcgaccgcgt cttcttcccg taccaggcct tgaagcgctc
     1861 gtcattggcc cgacttggca cctggagcag gacatgatcg ccgctgcccc agcggtcagc
     1921 gacattgcgg ttcagctcat cggcggcctc aggtggcaat ccgatcgggt agtcggcgga
     1981 ggccacgtat ttgctgctgg tgttgaccag gatcaatgca gcggtgcgct ctggcttggt
     2041 ggccgcgaac agcattccca tgggtccggc gtcgtaggtg gtcattaggg cggcccgttg
     2101 gctcccgacc tcctccatca cacattccaa ctcctctaca aaggactccc aagggggcag
     2161 ggcgtcgagg gggagcgggt cggaggcgcc ggtgccacgc cggtcgaagc ggatgaccct
     2221 ggcgaacgac gcgagtcgcc tgaagaagag ctctgcctgc gggtcctccc aatcggagtc
     2281 gaaggccccg aagctccccg cggttatgac caggtcgatc gatgaatccg ggctctcgcc
     2341 gaagacctcg taggcgatgc gatccgagcc aagac
//
LOCUS       DBNHXU010000104         1565 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1026446, whole genome shotgun sequence.
ACCESSION   DBNHXU010000104 DBNHXU010000000
VERSION     DBNHXU010000104.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1565)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1565
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1026446"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..197
                     /locus_tag="AC161X_02745"
     CDS             <1..197
                     /locus_tag="AC161X_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988499.1"
                     /translation="HTDRALPGHYLSFVDEGGLLTTLQLAAFGEEIDVFVEGDAFKTE
                     HRFSLGNVGFMTLHYDITRR"
     gene            complement(202..1152)
                     /gene="yedA"
                     /locus_tag="AC161X_02750"
     CDS             complement(202..1152)
                     /gene="yedA"
                     /locus_tag="AC161X_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013157482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="drug/metabolite exporter YedA"
                     /protein_id="HIR4988500.1"
                     /translation="MAESALTTSSPKPSRGKIAAALVAVYFIWGSTYLGLKFGLEGFP
                     PFLLNGIRFVIAGSIMFLVLRIRGHGSPTPRQWRNLVLMGIPLFVGGLSLVTVAESLG
                     VGSGLTATAIAMTPVWAALWSGLFGKWPVRREWIGLIVGLLGVLVLAQEGDFRASAVG
                     TLLVILSPIFWSFGSIWGPRLDLPDGLMAPAAQLFSGGFLLLLIGLVRGERIVEAPPT
                     AAWLALAYLIVFGSIIAYTSFFYLIRTVRPALALSYPYVNPMVAVALGVTLGGEFVTG
                     PIFIALPLILASVGLVATARRDTSVAATRPQPNAPVEEAA"
     gene            1231..>1565
                     /locus_tag="AC161X_02755"
     CDS             1231..>1565
                     /locus_tag="AC161X_02755"
                     /inference="COORDINATES: protein motif:HMM:NF018984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HIR4988501.1"
                     /translation="MDFSHYSGQPVDMAVDLVNTLDAASGEEKMAAPGDVATFIEAYD
                     SEDWCRPGWEPTERDLHEVRALRSRLRDVFETDDQAVAAAVLNGILSDVHAVPRLSVH
                     GIGPHLHFES"
BASE COUNT          329 a          469 c          511 g          256 t
ORIGIN      
        1 cgcacaccga tcgggccttg cccggccact acctgtcatt cgtcgacgag ggtggcttgc
       61 ttaccacgct gcaacttgct gcctttggtg aggaaatcga tgtcttcgtc gaaggtgatg
      121 ctttcaagac cgaacatcga ttcagtctgg gcaacgtcgg tttcatgacg ctgcactacg
      181 acatcacccg tcgctagtcg gttaggcggc ttcctccacg ggtgcgttcg gctggggcct
      241 ggtggcggcc acagaggtgt cgcgccgcgc ggtggcgacc aacccgacgc tggcgaggat
      301 caacggcagg gcgatgaaga tgggccccgt cacgaattct ccgcccagtg tcacaccaag
      361 agcaaccgcg accatggggt tcacatacgg gtagctgaga gccagcgccg gccgtaccgt
      421 tcggatcaga tagaagaacg aggtgtaggc aatgatcgag ccgaaaacga tcaggtaggc
      481 gagggcgagc caggctgcgg tcgggggtgc ttcaacgatc cgttcacctc gaaccagtcc
      541 gatgagcagt agaaggaacc cgccggagaa gagttgggcg gcgggcgcca tcaggccatc
      601 gggcagatcg agtcgcggac cccagatcga gccaaacgac cagaagatgg gcgacagaat
      661 caccagcagg gtgccgacgg cgctggcacg gaagtcgccc tcctgggcga ggacgagaac
      721 acccaggagg ccgacgatca gcccgatcca ctcacggcga accggccatt tcccgaacaa
      781 accgctccac agtgcagccc acaccggcgt catggcgatc gcggtggcag tgaggccgga
      841 accgacaccg aggctctcgg caaccgtcac cagactgagc ccgcccacga acagcggaat
      901 ccccatgagg acgagattcc gccactggcg gggggtgggg gagccgtggc cacggattcg
      961 gaggacgagg aacatgatgc tgccggcgat gacgaatcgg atcccgttga ggaggaacgg
     1021 tggaaaccct tcgaggccga acttcagacc gagataggtc gatccccaga tgaagtacac
     1081 ggcgaccagg gcggccgcga tcttgccccg ggatggcttg ggtgaggatg tggtgagcgc
     1141 tgactcggcc atgtcgttct atcggtctcg gatgtagcgg gctatactga gttcgtacct
     1201 tacactatac ttgaaggagt aagggctcaa atggattttt cgcattacag cgggcaaccc
     1261 gtcgacatgg cggtggatct ggtgaacaca ctcgacgccg ccagcggcga ggagaagatg
     1321 gcggcgccag gcgatgtcgc caccttcatc gaggcttacg acagcgaaga ctggtgtcgc
     1381 ccaggctggg agccgaccga gcgcgacctc cacgaggtcc gtgctctccg ctcacggctc
     1441 cgcgatgtgt tcgagaccga cgatcaggct gtcgccgccg ccgtgctcaa cggaatcctc
     1501 tcagatgtgc acgcggtgcc caggctgagc gtccacggaa ttggccctca ccttcatttc
     1561 gaatc
//
LOCUS       DBNHXU010000105         1917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4001527, whole genome shotgun sequence.
ACCESSION   DBNHXU010000105 DBNHXU010000000
VERSION     DBNHXU010000105.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1917
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4001527"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..499)
                     /locus_tag="AC161X_02760"
     CDS             complement(<1..499)
                     /locus_tag="AC161X_02760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012377770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR4988502.1"
                     /translation="MQTARNRLRIVGVATALALLLAACGSGETTDTTVAEPATPTTEA
                     MTNTTMAMTDMTQANMGAGCSAVPAEGAGSFDGMALDPAATAASNNPALSTLVSAVSE
                     AGLVDTLNGDGPFTIFAPTNDAFAAIPEADLSAILADQDLLTSILTYHVVAGESLDAQ
                     ELIDAG"
     gene            723..1271
                     /locus_tag="AC161X_02765"
     CDS             723..1271
                     /locus_tag="AC161X_02765"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR4988503.1"
                     /translation="MADLNRNDANLVNRIAGRDQEALAEAFHAYGGAVKSVAFRVIRD
                     QALAEDVVQDTFVALWRSPEKFDPVRGSLRTFLLTIAHRRAVDIIRSEEARSRREALP
                     PDPDHFDLEDEVWTRRLSADVRSALVDLSTDERQAISLAYFEGLSYVEVAKHLGAPEG
                     TVKSRIRTGMRKLADSLEGVAS"
     gene            1268..>1917
                     /locus_tag="AC161X_02770"
     CDS             1268..>1917
                     /locus_tag="AC161X_02770"
                     /inference="COORDINATES: protein motif:HMM:NF052375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HIR4988504.1"
                     /translation="MMDHDQVLELVPLHALDALDEVEAREVEAHVATCDVCSSELANH
                     RAVASGLVADEAAPSGVWERIVGEIGEPASATVTNIDEARSSTKRRVTWLTSVAAIAA
                     LVFGVVAIVQTAAKNELTGPEGIVAAAEAAAQEPGAIVTEFTTESGTVGQVVLTADGD
                     GYVIPSELEPLGADRTYQLWVITPDEVAISAGVLGNEPGPSRFTWTADVAGFALTR"
BASE COUNT          344 a          572 c          634 g          367 t
ORIGIN      
        1 tcccggcgtc gatcaactcc tgggcgtcca aggactctcc ggccaccacg tggtaggtga
       61 ggatcgaggt gagtagatcc tgatccgcga ggatcgcgct cagatccgct tcgggaatgg
      121 cggcgaatgc gtcattggta ggagcaaaga tcgtgaacgg gccgtcaccg ttgagggtgt
      181 cgacgagccc ggcctcgctc accgccgaca ccagggtcga caacgctggg ttgttcgagg
      241 ctgcggtggc ggcaggatcg agggccatcc catcgaagga gccggcgccc tcggccggca
      301 ctgcagagca gccagcaccc atgttggctt gtgtcatgtc agtcattgcc atcgtcgtgt
      361 tggtcatggc ctctgttgtt ggtgttgcgg gctcggccac cgtggtgtcc gtcgtctctc
      421 cgctcccgca agcggcgagg aggagggcga gcgcggttgc gactcccaca atcctcagtc
      481 ggtttcgtgc tgtttgcatg ggcgttcctt tcgggttggg ttgcagaacg ccgggtattc
      541 gttgcagacg gcgagacaga tgactgaggc tcggtcgacc cgttgctcgc ggatgagatc
      601 atccgggggg gtgtgtggtg tcgaatggcg ggtggatcag gaggatccca cgattgctcg
      661 agaacatgcc agcgaccacc caaacgtatc atcatcggtc cggagcccga tcggagactc
      721 atttggcgga cctgaaccga aacgatgcga acctggtcaa ccgcatcgcc ggtcgagatc
      781 aggaagctct agccgaggcc ttccatgctt acggcggggc tgtgaagtca gtcgcgtttc
      841 gggtgataag ggaccaagct ttggccgagg acgtcgtaca ggacaccttt gtcgcattgt
      901 ggcgatcacc ggagaagttc gacccggttc gggggtcgtt acgcaccttt ctactcacga
      961 tcgcccaccg acgcgcggtc gacatcatca ggtcagaaga ggcgcgctcc cgacgtgagg
     1021 ccctccctcc cgatccggat cacttcgatc tcgaggacga ggtctggaca agacgtctct
     1081 ccgccgatgt caggtccgct cttgtcgacc tctccacgga tgagaggcag gcgatctccc
     1141 tggcctattt cgaaggtctc agctatgtcg aggtggccaa acacctcggg gctcccgaag
     1201 gcaccgtcaa gagcaggatt cgcacaggta tgagaaaact cgcggactcg ctggaagggg
     1261 tggcgtcatg atggatcatg atcaagtcct cgagctagtg cctctccacg ccctcgacgc
     1321 actggacgaa gtcgaggccc gggaagtcga agcccatgtc gcgacctgtg atgtctgcag
     1381 ttctgaattg gcgaaccacc gcgccgtggc tagcgggctc gtcgccgacg aggcagcgcc
     1441 ctcgggagta tgggagcgga tcgtcggcga gatcggggag ccggcgtcgg cgaccgtgac
     1501 gaacatcgac gaagctcgtt cctcgaccaa gcgtcgtgtc acctggctca catccgtcgc
     1561 ggcgatcgcg gcactggtat ttggagtggt tgccatagtt cagaccgcag ccaagaacga
     1621 gctgaccggg ccggagggaa tcgttgccgc cgccgaggcc gccgcccagg aaccgggggc
     1681 gatcgtgacc gagttcacca ccgagtcggg gacggtcggc caggtcgttc tcaccgccga
     1741 cggcgatggc tacgtcattc cgtccgagct ggagcctttg ggtgccgacc gcacttatca
     1801 gttgtgggta ataacccccg atgaggtagc gatctcagct ggagtgctcg gaaatgagcc
     1861 gggtccctcc cgtttcacct ggactgccga cgtcgccggc ttcgccctca cccggga
//
LOCUS       DBNHXU010000106         1556 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_232676, whole genome shotgun sequence.
ACCESSION   DBNHXU010000106 DBNHXU010000000
VERSION     DBNHXU010000106.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1556)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1556)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1556
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_232676"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(119..1105)
                     /locus_tag="AC161X_02775"
     CDS             complement(119..1105)
                     /locus_tag="AC161X_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium and cobalt transport protein CorA"
                     /protein_id="HIR4988505.1"
                     /translation="MIVDSALYKDGIRLPGPFVLEGAHDFCNGTNTFAWIGLYEPTEE
                     EFEAVRKEFGLHELAVEDAIHAHQRPKLDVYGETLFVVLKPARYVDSVEVVDFGEILL
                     FVDQNFVVAVRHREPSRLVEVRKSLEADPRQLALGPSAVLHAVVDKVVDDYGQVLEGL
                     ELDIGELEAQVFSDEAAPTERIYKLKREVLQFHQATQPLLEPLERLIAGGFAVLSPEL
                     RQYFRNPHDHLLRVVERVAGFRDLLTSILEANLIQVTVRQNEDMQRQNEIVKKVSSWA
                     AIIAVPALITGYYGMNVPYPGSGSVWGVVTSTLLILLLSLGLYLEFRRRDWL"
     gene            complement(1125..>1556)
                     /locus_tag="AC161X_02780"
     CDS             complement(1125..>1556)
                     /locus_tag="AC161X_02780"
                     /inference="COORDINATES: protein motif:HMM:NF016202.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanW family protein"
                     /protein_id="HIR4988506.1"
                     /translation="GVSQFGTTMYNAIFWGGYEIIDHQPHSGWISRYPLGIEATLVYS
                     SIDLKFKNDTVTPVYVRTSTTSTSLTVELWGYQGGWRIRGSHPSGSRSSSITILDRGG
                     SDAKRVSARVTGSAPGAVRVIRTLTQGGVAHAQTWNWFYLS"
BASE COUNT          334 a          456 c          459 g          307 t
ORIGIN      
        1 accgggaagc catcggtcag gtattggccc ggggggagcc tcgcggagtc ttccacctcg
       61 ggacggcggc cgaagaagcc tctggaaatg atgggcactc atcgcctcct atgggtggtc
      121 acagccagtc acgacgacgg aattcgagat agagacccaa cgagagcaac agaatcaaca
      181 gcgtggaagt gacgacaccc cagacgctgc cggagccggg gtagggcacg ttcatgccgt
      241 agtagccggt gatgagtgcg gggacggcaa ttatcgccgc ccagctactc accttcttga
      301 cgatctcatt ctgacgctgc atgtcctcgt tttgacggac ggtcacttga atcagattcg
      361 cttccaggat ggaagtgagg aggtcgcgga agccggcgac tcgctccacc acacgtaaaa
      421 gatggtcatg agggttgcgg aagtactggc ggagctctgg gctcaaaacg gcaaaaccgc
      481 cagcgatcag tcgctccaga ggctcgagca ggggctgggt ggcctggtgg aactggagta
      541 cttctctctt gagtttgtaa atgcgttcgg ttggcgccgc ctcatctgag aacacctgtg
      601 cttccaattc tccgatgtcc agttctaatc cttcgagaac ctggccgtag tcgtccacca
      661 ccttgtcgac cactgcatgc aataccgcgc tgggtcccaa cgccaactgg cgggggtcgg
      721 cctcgagact cttacggacc tcgaccagac gagagggctc gcgatgacgc acagccacga
      781 cgaagttctg gtcgacaaac aacagaatct cgccgaaatc cacgacctcg actgaatcga
      841 catatcgggc cggcttgagc acgacaaaca aggtctcgcc atagacatcg agcttcggtc
      901 gctgatgagc gtgaatcgca tcctcgaccg ccagctcgtg aaggccgaat tcctttcgaa
      961 ctgcttcgaa ctcctcttcg gtcggttcgt ataggccgat ccaagcgaag gtgtttgtcc
     1021 cgttgcagaa gtcgtgggcg ccttccagaa cgaacggtcc tgggagccga atcccgtctt
     1081 tgtaaagggc agagtcgacg atcatgcgcg caggctacgc gggatcagct caggtagaac
     1141 cagttccagg tttgggcgtg agccacgcct ccctgggtca aggtgcgaat gaccctcact
     1201 gctcccgggg ccgagcccgt aactctcgcc gacacacgct tggcatcgga tccgccccgg
     1261 tcgaggattg tgattgacga gctccggctt ccgctgggat gtgatcccct gatcctccag
     1321 ccgccctgat atccccacaa ctcgacagtc aacgaagtcg aggtggtcga agtgcggaca
     1381 tacaccggag tcaccgtgtc attcttgaac ttgaggtcga tggaggaata gacgagcgtc
     1441 gcttcgatgc caagtggata acgcgagatc cacccgctgt gtggctgatg gtcgatgatc
     1501 tcgtacccac cccaaaagat ggcgttgtac atggtggtcc cgaattggct cacccc
//
LOCUS       DBNHXU010000107         3479 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1423234, whole genome shotgun sequence.
ACCESSION   DBNHXU010000107 DBNHXU010000000
VERSION     DBNHXU010000107.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3479)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3479)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3479
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1423234"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            18..149
                     /locus_tag="AC161X_02785"
     CDS             18..149
                     /locus_tag="AC161X_02785"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988507.1"
                     /translation="MVVLTAHAEEESRRRANEGGADAFVTKPFQPNDFRSTVLSLLE"
     gene            182..2440
                     /locus_tag="AC161X_02790"
     CDS             182..2440
                     /locus_tag="AC161X_02790"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR4988508.1"
                     /translation="MSDPFLPSFIGAPVQRREDPALITGTARYVDDISPAGTLHLAVV
                     RSPFAHARVTSIDIEAALEANGVRLVITPDQVSEVMMPPTPDASRNIPRRFPLVQGTV
                     LMPGDPVAAVVADTAAAARDGADLVFVDYDPLPIAGDVDQAIDAAPVHDGQASNVAYD
                     RARGDRAQFDALQGVIRLSGLVDHPRVAPAPMEPRAILAEWMEGGLTVHLSSQAPHLM
                     QEEFAKSFGMPQSSVRVITPFVGGGFGCKFDLAEEDYLTVIAAKETGRPVKWIETRRE
                     HLLAIGHGRAQRHRWEVVADAGGKIEGLWVDSRLDLGCRHRYLGFVDVTPRIGTGNYD
                     IPVYGWRQRGVWTNRAPRGIYRGAGRPEATLTIERVIEAVAKATGIDPAEVRRRNFVQ
                     PSQFPYRSRGGYTYDTGEYEKALDKLLEVADYDGLRARQTELRAQGRLMGIGLAAYVE
                     VCGFEDWGAARIQVHGDGSVTLYAETLDQGQGHRTTFPQIVAASLGISLDQVRLEQGD
                     TSSAPYGWGTSGSRSVAQGGAASFAAAEKVAAKAKRVAAHLMEAAAEDITLAEGVGTV
                     TGTDVSVTWREIATAAWAGNVPEGDAPGLDEEVHLKSGGFNYPFGMHLAVVEIDSETG
                     APTMSAMWAVDDAGNIINPMLADGQRHGGLAQGIGQALWEEVIYDEDGNLVTSSFMDY
                     LIPTAGSLPMFSLGATVTTTPTNPLGAKGIGEAGAIGSTPAVLNAVADALGVDEIQVP
                     LTAQKIWKALSR"
     gene            complement(2437..3459)
                     /gene="gap"
                     /locus_tag="AC161X_02795"
     CDS             complement(2437..3459)
                     /gene="gap"
                     /locus_tag="AC161X_02795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006930994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR4988509.1"
                     /translation="MVNVAINGFGRIGRAVFRIIASRPDSGISVVAINDLSDDDILAY
                     LLEYDSVMGRFDQEVTVDDGVMKVGPHEIQMLMERDPAQLPWTELGVDVVIESTGVFR
                     DRAALQKHIDAGAKRVILTVPSQDKIDQTVVLGVNDEELGADDIIVSNASCTTNCLVP
                     LVKVLDDNFGIIKGVMTTVHAYTNDQVLADVPHKDLRRSRAATENIIPTTTGAAKAVG
                     EVLPSMDGKLDGIAMRVPVPDGSTVDFVAELSRDVTVEEVNAAVKAASEGRMAGILEY
                     TEDPIVSTDIIGNPHSSIFDAAGTQVLGGNLVRVMSWYDNEWGYSNRVVDLIERLAEV
                     MAKQPA"
BASE COUNT          620 a         1082 c         1139 g          638 t
ORIGIN      
        1 cgaccaagaa catccctgtg gtggtcctga ctgcccatgc cgaggaagag tcgaggcggc
       61 gtgcgaacga aggtggtgcc gacgcattcg tcaccaagcc cttccagccg aacgacttcc
      121 gcagcacagt cctctcgctc ctcgaataag aaccggcggc ctgcccggta ggctgccgtc
      181 tgtgagcgac cccttcttac cctcatttat cggcgcgccc gtccagcggc gcgaagaccc
      241 ggcgctgatc accgggacag cccgatacgt cgacgacatc agtcctgccg gcaccctcca
      301 cctggcggta gtccgaagcc ctttcgctca cgccagagtc acttcgatcg atatcgaggc
      361 cgccctcgag gccaacggcg ttcgtctggt gatcactcct gaccaggttt cggaggtgat
      421 gatgccaccg acccccgacg cttccagaaa cattccccgt cggttccctc tggtccaagg
      481 cacagtcctg atgcccggcg atccggttgc cgcggttgtg gccgacaccg cggccgcggc
      541 cagagacggg gcggacctgg tgttcgtcga ttacgacccg cttccgatcg ctggcgatgt
      601 cgaccaggcg atcgacgccg ccccggttca tgatggccag gcctcgaacg ttgcctacga
      661 ccgggcacga ggtgatcggg cgcagttcga tgcccttcag ggcgtgatcc ggctttcagg
      721 tctcgtcgat cacccccggg tggctccggc cccgatggaa cccagagcga tcctggccga
      781 atggatggag ggtgggctga ctgtccatct ctccagccag gcgccgcatc tgatgcagga
      841 ggagttcgcc aaatcgttcg gcatgcccca gtcctcggtt cgggtcatca ccccattcgt
      901 gggtggaggc tttggctgca agttcgatct cgccgaggag gactatctga ctgtcatcgc
      961 ggccaaagag accggccggc cggtcaagtg gatcgagacc cgacgcgagc atctgttggc
     1021 catcggtcac ggacgggcgc agcgtcatcg ttgggaggtg gtggcggatg ccggaggaaa
     1081 gatcgagggt ctgtgggtcg acagccgcct tgacctggga tgtcgtcatc ggtacctcgg
     1141 ctttgtcgat gtcacacccc gcatcggcac cggcaactac gacattccgg tctatggctg
     1201 gcgccagcga ggggtatgga ccaatcgggc cccacgcggg atctatcggg gcgctggacg
     1261 acctgaggcg accctgacca tcgagcgggt catcgaggcg gtggccaagg caaccgggat
     1321 cgatccggcc gaagtgagac ggaggaattt cgtccagccc tcgcagttcc cctatcgatc
     1381 tcgaggcggt tacacctacg acaccggaga gtacgagaag gccctcgaca agcttctcga
     1441 ggttgcggac tacgacgggc tccgagctcg ccaaaccgag ttgagggcgc agggacgtct
     1501 gatgggaatc ggcctcgccg catacgtcga ggtgtgcgga ttcgaggatt ggggagcggc
     1561 ccggatccag gtgcatggtg acggatcggt caccctctac gcggagacgc tcgaccaggg
     1621 ccagggtcac cggacgacct tcccgcagat agtggctgcc agtctcggga tctcgctgga
     1681 ccaggtgcgg ctggagcagg gagacacttc ttcggcgccc tacgggtggg gaacgtcggg
     1741 atctcgcagc gtcgcccagg gaggcgccgc cagctttgct gcagcggaga aggtcgccgc
     1801 caaggcaaag agggtggcgg cccacttgat ggaggctgcg gccgaagaca taactctggc
     1861 cgaaggagtc ggcactgtca ctgggaccga tgtctcagtg acctggcggg aaatcgcaac
     1921 cgccgcctgg gccggcaatg tgccggaggg ggatgccccc gggctcgacg aggaagtcca
     1981 cctcaaatcg ggcgggttca actacccatt cggcatgcat ctcgcagtgg tcgagatcga
     2041 ctccgagact ggagcgccga ccatgtcggc gatgtgggcg gtcgacgacg cggggaacat
     2101 catcaacccg atgttggccg acggccagcg tcacggtgga ctggcccaag ggatagggca
     2161 ggcgctgtgg gaggaggtca tctacgacga ggacggcaac ctggtcacct cgtcgttcat
     2221 ggactatctg atccctaccg ccggatcgtt gcccatgttc tctctgggtg cgaccgtcac
     2281 gacaactcca accaatccac tcggggccaa ggggataggc gaggccggcg ccatcgggtc
     2341 aacgccggca gttttgaacg cggtcgccga cgcactcggc gtggatgaga tccaggtgcc
     2401 tctcaccgcc cagaagatct ggaaagcgct gtcgcgttag gcgggttgct tcgccatcac
     2461 ctcggcgagg cgctcgatga ggtcgaccac ccggttggag taaccccact cgttgtcgta
     2521 ccaggacatc actcgcacca ggttgccccc gagcacctgc gtgccggcgg cgtcgaagat
     2581 cgaggaatgc ggattgccga tgatgtcagt ggagacgatc gggtcctccg tgtattcaag
     2641 gatgccggcc atccggcctt ctgatgcagc cttcaccgcg gcattcacct cttcgaccgt
     2701 gacatctctc gacagttcgg ctacgaagtc gaccgtcgag ccgtcgggca ccggcactcg
     2761 catcgcgatg ccgtcgagct tgccgtccat gctgggcagg acctcgccga ctgccttggc
     2821 ggcaccggtg gtcgtcggga tgatgttctc tgtcgccgca cggcttcgcc tgaggtcctt
     2881 gtgggggaca tcggcgagga cctggtcgtt ggtgtaggcg tggaccgtgg tcattacgcc
     2941 cttgatgatc ccgaagttgt catcgagaac cttgaccaga ggaaccaggc agttcgtggt
     3001 gcaggaggca ttggagacga tgatgtcgtc ggctccgagc tcctcgtcgt tgaccccaag
     3061 gaccacggtc tgatcgatct tgtcctggga gggaacagtg aggatcacac gcttcgcccc
     3121 agcgtcgatg tgcttttgaa gcgccgccct gtcccggaag acgccagtgg actcgatgac
     3181 gacgtccact ccgagctcgg tccatgggag ttgggcgggg tcgcgttcca tgagcatctg
     3241 gatttcgtgg gggccgacct tcatcacgcc gtcatcgacc gtcacctcct ggtcgaaccg
     3301 acccatgacc gagtcgtact cgaggaggta ggcgaggatg tcgtcgtcgg aaaggtcgtt
     3361 gatggcgacg acgctgatcc cggagtcagg ccgggaggca atgatccgga acacggctcg
     3421 gccgatcctc ccgaatccgt tgattgcgac gttcaccatc ttcagtgtcc tccatctag
//
LOCUS       DBNHXU010000108         7037 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_233754, whole genome shotgun sequence.
ACCESSION   DBNHXU010000108 DBNHXU010000000
VERSION     DBNHXU010000108.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7037)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7037)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7037
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_233754"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="AC161X_02800"
     CDS             <1..899
                     /locus_tag="AC161X_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003798628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988510.1"
                     /translation="LTDEDPETVAAERSRIATEGWGARLLALQEPEGQWDGGTYQPGW
                     ADPYVYVPAWTSTTYTLLLLRDLGLDPASEQAQHAVALVRDNSKWEHDGQDFFDGEVE
                     PCINGIAVALGSYFGQEVGSIVERLLGDQMADGGWNCEQESGSTRGSFHSTISVLEGL
                     LEYERATDSPAEVIAARRRGEEYLLERRLWRRLSGGEVVDPAFALFSYPTRWHYDVLR
                     GLDYLRTAGSPPDDRISEALEVVNRNRGEDGRWPLQNTHPGPVHFEMDQGDGEPSRWN
                     TMRAMRVMVWATPIGDGVPPLG"
     gene            complement(928..1491)
                     /locus_tag="AC161X_02805"
     CDS             complement(928..1491)
                     /locus_tag="AC161X_02805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988511.1"
                     /translation="MTTTAKSASVRLHPVVKGALLGLSWGIAMRVWMRFITTNPEFTW
                     TGTGYILGATTLAGMLLGLGWARRAKAKGNWWRLTGLSMLPLGVAAGGVMIPSVFLGA
                     LALGRRNWHMAVRGLLLIVAVGFQVFAFTVGPGDGFPSNKVVPAMVVYGVLISIEALA
                     FSIPFLPSRVAEPFIEALEPLPATGGD"
     gene            complement(1570..3921)
                     /locus_tag="AC161X_02810"
     CDS             complement(1570..3921)
                     /locus_tag="AC161X_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011874117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4988512.1"
                     /translation="MHTADSHDFIQVTGARENNLKDVSVQIPKRRLTVFTGVSGSGKS
                     SLVFDTIAAESQRLINETYSAFVQGMMPTMARPDVDMLDGLTTAIIVDQRRMGANARS
                     TVGTATDTNAMLRLLYSRLGEPHIGPPPAFSFNIPTRTASGAITADKGKGERTVVRNV
                     VYQGGMCPRCEGMGSVSDIDLTKIYDESKSLNEGAITVPGYKAGGWSVRYFTDSGFLD
                     PDKPINKYTKKELQDFLYKEPVKVRINDINLTYEGLVPQIQKSFLSKDVDTLQPHIRD
                     FVERAVAFTVCPDCDGTRLNEAARSSKIAGNSIADLCAMQISDLAEWIRELGEPSAKP
                     LLGALQDTLDSFVDIGLGYLSLDRPAGTLSGGEAQRTKMIRHLGSPLTDVTYVFDEPT
                     IGLHPHDVQRMNDLLMQLRDKGNTVLVVEHEPETIEIADHVVDLGPRAGTGGGEVVFE
                     GTVDELRRSGTLTGKHLDDRSSVKSEFRNSVESLEIRHANRNNLQDVDVDIPLGVLVV
                     VTGVAGSGKSSLVQGSIPEGEGAVLVDQTEIRGSRRSNAATYTGMLDSIRNAFAKANA
                     VKPGLFSPNSDGACLNCNGAGVIYTELGFMDTVATTCEVCEGKRFGAEVLEYQLGGKD
                     ISEVFEMSVIEAEKFFAEGESKVPAAHAILERMSAVGLGYLSIGQPLTTLSGGERQRL
                     KLAIHMAEKGGIYVLDEPTSGLHLADVEHLLGLLDRLVDSGKSVIVIEHHQAVMAHAD
                     WIIDLGPGAGHDGGQVVFEGTPADLVAARSTLTGEHLAAYVGA"
     gene            complement(3932..4435)
                     /locus_tag="AC161X_02815"
     CDS             complement(3932..4435)
                     /locus_tag="AC161X_02815"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007923113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR4988513.1"
                     /translation="MSVTGSPQTISWAEFAQREAELARFGADRLTAAPAYLATVRRSG
                     APRVHPVTPIFTELGLFLFMEPTSPKGGDLRERGWFALHSGVPDNAGTGGEFCATGRG
                     IAIDDPALWSQAADAASYSPIDRYVLFEFELSEARCHGYGDVPLPPTKKWSVGQDVSS
                     RVADRPI"
     gene            complement(4460..4864)
                     /locus_tag="AC161X_02820"
     CDS             complement(4460..4864)
                     /locus_tag="AC161X_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011773069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4988514.1"
                     /translation="MTVSISHVHVLVDDPDAAVAFYRDTLGLTVLNEVENGGFRWITL
                     ATDSQPEIQIVLSQPQAGRSEEDGNAIAALLAKGELGSVFFRSDDLNETFDKVAAAPG
                     VEVLSEPADQFWGARDAAVRDPAGNMIRIEQG"
     gene            complement(4875..5321)
                     /locus_tag="AC161X_02825"
     CDS             complement(4875..5321)
                     /locus_tag="AC161X_02825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020525133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4988515.1"
                     /translation="MSADLDELRWLRRARDQMDREYAQPLDVPALARTAMMSPAHFSR
                     RFRAAYSETPYSYLMTRRIERAKALLRQGDKSVTEVCFEVGCTSLGSFSTRFTELVGE
                     TPSAYRARDHDEVRSIPSCQTMVASRPRRERAVSSSFGEASVTSLS"
     gene            complement(5346..5729)
                     /locus_tag="AC161X_02830"
     CDS             complement(5346..5729)
                     /locus_tag="AC161X_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020417887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4988516.1"
                     /translation="MSGRVVHFEIPYDEGERSRNFYSAVFGWEVTEMPEMSYTFVVSG
                     PSGEEGPTEPGFINGGMMERKDPFTSPNLVVEVESIEESLKAVREAGGTTVSDREQVG
                     DMGFAAYFKDTEGNLVGLWETASRS"
     gene            complement(5807..>7037)
                     /locus_tag="AC161X_02835"
     CDS             complement(5807..>7037)
                     /locus_tag="AC161X_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017834622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR4988517.1"
                     /translation="FLDISDFPTRHNYILALWQSRIEPILASWVEELGVPILRQRELV
                     GFIQDDTGVDVELSADTRLRAEYLVGCDGGRSLIRKGAGIDFPGWDPTASYMIAQVEM
                     EDEPEVGMRPEGGGIGPVNREKGGSPYGVVLPENEIEHTSEPTIEDLRELLVDAYGTD
                     YGAHSPIWISRFTDMCRQAAVYRKGRVLLAGDAAHVHGPQGGQGLNTGVQDAVNLGWK
                     LAQVVNGTSPESLLDSYHAERHPVGARVLQNTMAQVALARIDERSKALRDTMLEQLSM
                     DEPRKRIVGMISGLDIHYDLGEGHPLLGRRMPDFDLHTSDGPTRVFALLHEARPVLLN
                     LDDPGGFDVSPWSNRVRLVDATHDGGWELPVLGEVSPPPAVLIRPDGYVAWTGDLADP
                     ELPRALATWFGAPTPHR"
BASE COUNT         1276 a         2205 c         2252 g         1304 t
ORIGIN      
        1 atctcactga cgaggatccg gagacggtgg ccgcagaacg ctcacggatt gccaccgaag
       61 ggtggggtgc caggctgctc gcactccagg agcccgaggg ccagtgggat ggcggcacct
      121 accagccagg atgggctgat ccgtatgtgt atgtccctgc atggacctcc accacttaca
      181 cccttctgct tttgcgggac cttggtctcg atcccgccag cgagcaggct caacacgctg
      241 ttgcgctggt tcgggacaac agcaaatggg agcacgatgg ccaggacttc ttcgatggtg
      301 aggtagagcc gtgcatcaac ggcatagccg tggcgttggg ctcctacttc ggtcaggaag
      361 ttggaagcat cgtcgagagg ctgcttggtg accagatggc tgacggcgga tggaattgtg
      421 aacaggagag cggctccact cgagggtcgt tccattcgac gatctcggtg ctcgagggcc
      481 tcctcgaata cgaacgggca accgatagcc ctgcagaagt cattgcggcc cgccgccgcg
      541 gcgaggagta tctcctcgaa aggcggttgt ggcgacgtct ttctggtggc gaagtggtcg
      601 accccgcctt cgctctcttt tcctatccga cacgctggca ttacgacgtg ctcagggggc
      661 tcgactacct gcgaactgct ggctccccgc ctgacgatcg gatctcggag gcgcttgagg
      721 tggtgaatcg gaatcgggga gaggacggcc gctggcccct gcaaaacacc cacccgggcc
      781 ccgtgcattt cgaaatggac caaggtgacg gagagccaag tcggtggaac accatgcgtg
      841 cgatgcgggt gatggtttgg gcgaccccaa tcggcgacgg tgtgccgccg ctgggttagg
      901 agcttcagaa gtgacgagac agcgaaatca atcgccgccg gtcgctggga gtggttcgag
      961 agcttcgatg aaaggctcgg cgactcggga cggcaggaac ggaatcgaga acgcaagagc
     1021 ctcgatggag atcagaactc catagacgac cattgcaggc acgactttgt tcgatgggaa
     1081 accatcgccg ggaccgaccg tgaacgcaaa gacctgaaac cccactgcga cgataagtag
     1141 aagacctcga accgccatat gccagttcct tcggccgagg gcaagtgccc cgagaaagac
     1201 cgaagggatc atgacccctc ccgcggcaac acccaatggc aacatcgaaa gcccggtgag
     1261 ccgccaccag ttgcctttgg ctttggcgcg acgagcccag cccaaaccaa gcagcatgcc
     1321 agccaaggtc gtggcaccca ggatgtagcc ggttccggtc caggtgaact cagggttggt
     1381 ggtgatgaac cgcatccaga ccctcatggc tattccccag gagagaccga gcagcgcccc
     1441 cttcacaaca gggtggagcc gaaccgatgc actctttgct gtcgttgtca ctttcgcgta
     1501 cctccgtcag acagacgaag aaggtcggaa aatgggtgac acaccgactg ggaactgaat
     1561 cccgcgactt caggcgccga cgtaggccgc gaggtgctcg ccggtgaggg tggagcgagc
     1621 ggcaacgagg tcggccgggg tgccttcgaa cacgacctgg ccgccgtcgt ggccggcacc
     1681 ggggccgagg tcgataatcc agtcggcgtg cgccatgacc gcctggtggt gttcgatcac
     1741 gatcaccgat ttgccggagt cgaccagcct gtcgagtaaa ccgagaagat gctcgacatc
     1801 tgcgaggtgg agcccgctgg tgggctcatc gagcacgtag atcccgcctt tctccgccat
     1861 gtggatcgcc agcttgagcc gctgacgctc gccaccagag agcgtcgtca gcggctggcc
     1921 gatgctcaga tagccgagac ccaccgcgga catccgctcc aggatggcgt gggcggccgg
     1981 caccttcgac tcaccttccg cgaagaactt ctcggcctcg atgaccgaca tctcgaacac
     2041 ttcactgatg tccttgccac ccaactggta ctcgagcact tcggcgccga accgcttgcc
     2101 ctcacacacc tcgcaggtcg tggcgacggt gtccatgaag ccgagctcgg tgtagatgac
     2161 gccggcacca ttgcagttca ggcaggcacc gtcggagttg gggctgaaca gcccgggctt
     2221 gaccgcgttc gcctttgcga acgcattccg tatcgagtcg agcatcccgg tgtaggtggc
     2281 cgcgttgctc cgccgcgatc cgcggatttc ggtctggtcc accaagaccg ccccctcgcc
     2341 ctccggtatg gagccctgga cgagagagct cttcccggac ccggcgacgc cggtgaccac
     2401 cacgagtacc ccgagcggga tgtcgacgtc gacgtcctgg aggttgttcc gattcgcgtg
     2461 tcgaatctcg aggctctcca cagagtttcg gaactctgac ttgacggatg atcggtcgtc
     2521 gaggtgctta ccggtcagcg tgccgcttct ccgcaactca tcgactgtgc cttcgaacac
     2581 cacctcgcct ccgccggtcc cggcacgtgg ccccaggtcg acgacatggt cggcgatctc
     2641 gatggtctcc ggctcgtgct ccaccaccag caccgtgttt cccttgtcac gcagctgcat
     2701 gagcaggtcg ttcatgcgtt gcacgtcatg ggggtggagg ccgatcgtgg gctcgtcgaa
     2761 gacataggtg acgtcggtga ggggagaacc gagatgacgg atcatctttg tgcgctgtgc
     2821 ctcgccgccc gacagggtgc ccgccggccg gtccagggac aggtagccga ggccgatgtc
     2881 aacgaaggag tcgagtgtgt cctggagggc gccgagcaaa ggcttggccg acggctcgcc
     2941 gagctctcgg atccactcgg cgagatcgct gatctgcatt gcacagaggt cggcgatgct
     3001 gttcccggcg atcttcgacg aacgcgccgc ctcgttgagc cgggtgccat cgcagtcggg
     3061 gcagacggtg aacgcaaccg ctcgttccac gaaatcacga atgtggggct ggagcgtgtc
     3121 gacgtccttc gagaggaagg acttttggat ctgcggaacc agtccctcgt aggtcagatt
     3181 gatgtcgttg atcctgacct tcaccggctc cttgtagagg aagtcttgga gctctttctt
     3241 ggtgtacttg ttgatcggct tgtctggatc gaggaagcca gagtcggtga agtagcgcac
     3301 cgaccagccg cccgccttgt agccgggcac ggtgatggcg ccctcgttga gagacttgct
     3361 ctcgtcgtag atcttggtga ggtcgatgtc gctgaccgag cccatcccct cgcagcgtgg
     3421 gcacatgccg ccctggtaga cgacattgcg gaccactgtc cgctccccct tgcccttatc
     3481 ggctgtgatc gctccgctcg ccgtccgggt cgggatgttg aatgagaacg caggcggggg
     3541 cccgatgtgc ggctcgccga gccggctgta gaggagacgc agcatggcgt tggtatcggt
     3601 tgcagtgccg acagtggagc gggcgttggc gcccattcgc cgctggtcga cgatgatcgc
     3661 agtggttagc ccgtcgagca tgtccacgtc gggccgtgcc atggtcggca tcatgccctg
     3721 cacgaatgcg ctgtaggtct cgttgatcag ccgctgcgac tcggcggcga tcgtgtcgaa
     3781 gacgagggag ctcttccccg atcccgagac cccggtgaac accgtcaaac ggcgcttcgg
     3841 gatctgaaca ctgacgtcct tgaggttgtt ctcccgtgcc ccggtcacct ggatgaagtc
     3901 gtgactgtcg gctgtgtgca tcgggtccac gctaaatcgg cctgtccgca acacggctcg
     3961 acacatcttg gccaactgac catttcttcg taggaggaag gggcacgtcg ccatacccat
     4021 gacagcgggc ctcgctcagc tcgaactcga acaggacata acggtcgatc ggcgagtaac
     4081 tcgcagcgtc ggccgcttgg gaccacagtg ccggatcgtc aatggcgatt ccgcggccgg
     4141 tggcacagaa ctcaccacct gtgcccgcat tgtctggaac accgctgtgc aaagcgaacc
     4201 atccccgctc ccggaggtct ccccccttgg gggaggtcgg ctccatgaac aagaacaggc
     4261 ccaattccgt gaagatcggc gtcaccgggt ggactcgagg tgcccctgac cgccgaaccg
     4321 tcgccagata cgcgggggct gcggtcagtc tgtcggcccc gaaccgggcc aactctgctt
     4381 cacgctgggc gaactcagcc cagctgatcg tctggggaga tccggtgacc gacacgcccg
     4441 ataggaccct gatccggctc tagccctgct cgatgcggat catgttgccc gcagggtcgc
     4501 gcaccgctgc gtcacgggca ccccagaact gatcggcagg ctctgacagg acctcgaccc
     4561 cgggcgcggc ggcgaccttg tcgaaggtct cgttgaggtc gtcggagcgg aagaacaccg
     4621 agcccaactc ccccttggcg agcagagcag cgatcgcgtt cccatcctcc tcggagcgcc
     4681 cggcttgcgg ctgcgaaagc acgatctgga tctcgggctg gctgtcggtg gcgagggtga
     4741 tccatcggaa gccgccgttc tcgacctcgt tcaacaccgt gaggccgaga gtgtcgcggt
     4801 agaaggcgac ggcggcatcg ggatcatcga ccaggacgtg cacatggctg attgagactg
     4861 tcatgagcct gaccctacga gagcgatgtg acggatgctt ctccgaaact gctcgatacg
     4921 gcacgctccc gacgaggccg gctcgcgacc attgtctggc acgacggaat ggaacggact
     4981 tcatcgtgat cgcgggcgcg gtaggcgctc ggcgtctcac cgacgagctc ggtgaaccgg
     5041 gtggagaagg aaccgaggct ggtgcagccg acctcgaaac agacctctgt cacgctcttg
     5101 tcgccttgac gcagtagtgc tttcgcccgc tcgatccggc gggtcatgag gtaggagtag
     5161 ggcgtctcgg aataggcggc gcggaagcgg cgactgaaat gtgccggcga catcatcgcc
     5221 gtccgagcca acgccggcac gtcgagcggc tgggcgtatt cgcggtccat ctggtcccga
     5281 gcccggcgca gccagcgcaa ttcatcgagg tcggcgctca taggctcagc ctaccggtcg
     5341 acgattcacg acctggatgc cgtctcccag aggccgacga gattcccctc ggtgtctttg
     5401 aagtaggcgg cgaatcccat gtcgccgacc tgttcccggt cgctgacagt ggtccctccc
     5461 gcctccctca cggctttgag cgattcttcg atgctctcaa cctcgacgac gagattgggc
     5521 gaggtgaacg ggtccttgcg ctccatcatg ccgccgttga tgaatccggg ctctgtggga
     5581 ccttcttccc cactcgggcc cgagacgacg aaggtgtagc tcatttcggg catctccgtc
     5641 acctcccagc cgaacaccgc cgaatagaag ttgcggcttc gctcaccttc gtcatagggg
     5701 atctcgaaat ggaccactcg acctgacatc gacactcctc tcgtttgtcc aatttatacc
     5761 cgcgaatcga ggagaccatg cccgccggag caccccaacg ttcctcctat cgatgcggcg
     5821 tgggcgctcc gaaccaggtc gcgagagctc gaggcaactc tgggtcggcg aggtctcctg
     5881 tccaggcgac gtacccgtcg ggacggatca acacggcggg tggcggcgat acctcgccaa
     5941 ggaccggaag ctcccaccca ccatcgtgcg ttgcatcgac cagccggaca cgattcgacc
     6001 aaggagaaac gtcgaaaccg ccggggtcat cgaggttgag aaggacgggc cgagcctcgt
     6061 gtagcagggc gaagactcgg gtgggaccgt cggaggtgtg caggtcgaag tcgggcatgc
     6121 gccggccgag cagtgggtgc ccttccccga ggtcgtagtg gatgtcgaga ccggagatca
     6181 tgccgacgat gcgtttgcgg ggctcgtcca tgctcaactg ctccaacatg gtgtcgcgta
     6241 gggccttgct gcgctcgtcg atgcgggcga gcgcgacctg cgccatggtg ttctgcaaca
     6301 cccgggcacc gaccggatgc ctttcggcgt gataggagtc cagcaggctc tcgggtgatg
     6361 tcccgttgac cacctgggcc agcttccatc ccaggttcac ggcatcctgc acgccagtgt
     6421 tgaggccctg cccaccctgc gggccatgca cgtgcgccgc gtctccggcg agtagcacac
     6481 gtcccttgcg atagaccgcc gcctggcggc acatgtcggt gaaccgggag atccagatgg
     6541 gactgtgcgc cccgtagtcg gtcccgtagg cgtcgaccag cagctctctg aggtcctcaa
     6601 tggtgggctc gctggtgtgt tcgatctcgt tctctggcaa cacgaccccg taagggcttc
     6661 cacccttctc ccggttgacg ggaccgatgc cgccaccttc ggggcgcatc ccgacctccg
     6721 gctcgtcttc catctcgacc tgggcgatca tgtagctggc cgtcgggtcc caaccgggga
     6781 agtcgatgcc ggcccccttg cggatcaggc tgcgtcctcc atcacacccg acgaggtact
     6841 cggctctgag tctcgtgtcg gcggatagtt ctacatcgac gccggtgtcg tcttggatga
     6901 agcccaccaa ctcgcgctgg cggaggatcg ggacaccgag ctcttcgacc caggaagcca
     6961 ggatgggctc gatacggctc tgccagagcg ccaggatgta gttgtgacgg gtggggaagt
     7021 cgctgatgtc caagaag
//
LOCUS       DBNHXU010000109         7257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2218533, whole genome shotgun sequence.
ACCESSION   DBNHXU010000109 DBNHXU010000000
VERSION     DBNHXU010000109.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7257
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2218533"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="AC161X_02840"
     CDS             <1..417
                     /locus_tag="AC161X_02840"
                     /inference="COORDINATES: protein motif:HMM:NF021546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HIR4988518.1"
                     /translation="KKGSAAVLGFFESLVWVLAIARVLSGFDDPARVLAFALGFAAGT
                     YGGALVEEWLAIGQSLLRVIAPVETPSVAELLRARGHGATVVNGDGREGEVRITFCVV
                     PRRKVSEIVDLIETTNPDAFITVEQTTTVRLLQRHQ"
     gene            complement(438..809)
                     /locus_tag="AC161X_02845"
     CDS             complement(438..809)
                     /locus_tag="AC161X_02845"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR4988519.1"
                     /translation="MRKLTLAMTALALFLAACGGGSEATTTTAATGTTAPSDQVLITV
                     DSLSFPAEVTIPAGKSVAWKNEMLVIHQIVFDTHDGQPAGLDPMNLNGGATVEITLEP
                     GTWAYFCGLHSQMTGTLVVEG"
     gene            complement(843..2030)
                     /locus_tag="AC161X_02850"
     CDS             complement(843..2030)
                     /locus_tag="AC161X_02850"
                     /inference="COORDINATES: protein motif:HMM:NF017984.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR4988520.1"
                     /translation="MRMVGVDERRARLGIRHHLAEQSESTVAVVSDMVGLHSSDPATV
                     FLSSRARVTSLKVSDLESDLYENRSLARVLGMRRTLWVVPTESVTEVHNSSTIKIAGP
                     ELRRLANMVEGAGLSADGADWYRKVSEKTLAVIEARGEAMAAVELTKDVPELREQFTF
                     YKADGTVIGRTGASTRVLFALAAEGRVIRARPRGSWVSGQYRWAETADWLGAPIPAMP
                     KGAAQVEILGRLLLGFGPITEVDMAWWTGWTKGDVRIALRNLKAAEVETDIGPAYLHP
                     QDLEPVTASTSWVAFLPSLDPTTMGWKERGWYLGDHAPRVFDRNGNAGATVWCDGRIV
                     GGWSQRPGGEVRYRLLQDVGTDSSNAIEREAGSLQTGLDGTTVSARFPAPLDKLLAGQ
                     TPN"
     gene            complement(2065..2967)
                     /locus_tag="AC161X_02855"
     CDS             complement(2065..2967)
                     /locus_tag="AC161X_02855"
                     /EC_number="1.14.19.2"
                     /inference="COORDINATES: protein motif:HMM:NF015370.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045300 - stearoyl-[ACP] desaturase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP desaturase"
                     /protein_id="HIR4988521.1"
                     /translation="MARDEKYELVEAVDKVVDRLIKEHQQRRSDWYFHEFVPWERGES
                     FKEKPWDVSQCTISENARTALVLNLLTEDNLPYYHALIEKYMPPTSAFSAWNHLWTAE
                     EAQHAIAIRSYLLTSRNCDPYELEDDRKSTMINGYQPTFDDPTEIFAYTAAQELATRV
                     SHRNAGKITDDPAAFELMRLIASDENHHFIFYKSVMQAMLDSEPSLVLGGIFKVFDNF
                     QMPGAAMPRFVRRSIDVARAGVYNLRIHHDRVLVPLLKDWSIGDLTGLTTAAAEMQDK
                     IMALPAQIMERAENFERRVGIVPA"
     gene            3092..3937
                     /locus_tag="AC161X_02860"
     CDS             3092..3937
                     /locus_tag="AC161X_02860"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988522.1"
                     /translation="MTTRGVSRATRSGLSILGILVVFVLLWEGYKFIWTALEWTWPVR
                     PDNRTMPHTWDIIGALFQPAQRNGPLLLWVELKAALLTLREAFVGFLIGGAIGFGLGV
                     LFVRSTTAERAFMPFVVASQTVPIIAIAPMVVIWGGRLSVPQWLTVSVIAAYLTFFPV
                     VINTVRGLRSPEPTSMELMRSYASTPRETLWKVQVPSALPYIFTALKVSAAASVIGAI
                     VGELPSGFRGGLGRDLLQFSQQFAAAPQKLFAAVVLAGLAGIAFVGLITLTEHRLVAA
                     SRRQS"
     gene            3937..4716
                     /locus_tag="AC161X_02865"
     CDS             3937..4716
                     /locus_tag="AC161X_02865"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015236063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988523.1"
                     /translation="MPSAVEARGVGMVFGQGAESIEALKNINLTLSAGDFASLIGPSG
                     CGKSTLLRLIGDLATPSVGELAINGKTPEQARLDRDYGMVFQSATLLDWRTVEKNVEL
                     PLEIMGFDSAARAAKAQEMLSLVQLEQFADHYPWQLSGGMQQRVAIARALAFNPSLLL
                     MDEPFGALDEMTRELMQQEVLRIWEETETTVIFVTHSIPEAVFLSTRVLVMSPRPGSI
                     SDEISIDLATKRDEDTREATRYFQLITEVREALRRGERGDG"
     gene            4713..5582
                     /locus_tag="AC161X_02870"
     CDS             4713..5582
                     /locus_tag="AC161X_02870"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988524.1"
                     /translation="MTERDEVIRESHETSVITSFEGGGPVSTSRRSGLRRFGARYGPA
                     VLAFFAVILVWEGLTRLFSVQSFILPKPSEIMVSFFETSATIWGVGFNTLIEAVSGFL
                     LGTILAVATSLAAARWVGFREGMLPMAIALNATPIAALAPIFNNWFGLTNPFSKSGVV
                     AVVVYFPVMINTTRGLLEVDPAELELMRSVAASPVEVMRRVRIPNALPYFFASMKVAT
                     ALSLIAAIIAEYFGGPQDVLGQYIVNRAQLFQFPDAWAAILVASLIGVTFYALILLAE
                     RIFMPWHVSMRRS"
     gene            5656..6846
                     /locus_tag="AC161X_02875"
     CDS             5656..6846
                     /locus_tag="AC161X_02875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4988525.1"
                     /translation="MATTKWRHHVLWLAALAIVLAACGDTGTGSTTTEGTAEGTSTTA
                     GTGTTAAPGTTVAPDECTEPDPVRLQLQWFAQSQFAGYYVALDKGFYEAQCLDVTILE
                     GAVEIVPQQVLATGGAEFGLAWVPKALVSREGGADIVNIAQVFERSGTLQVSWADSGI
                     TEPADWAGKKVGNWGFGNEFELTAAISKFGVEDVELVGQDFTMTALLNREIDAAEAMI
                     YNEYAQVLEAVNPDTGELYQPEDLNVISYNEVGTAMLQDAVWVTSDWIADEANQDIAT
                     RFLTASFQGWIHCLDNFDECVQVVLDNGSTLGQSHQEWQLNEILGLIFPATNGIGVMN
                     TELWDQTVAVATEQIPELQGVEISADAYRTDLAEAAIAALGDADTSGAGFTRREVELR
                     ENGE"
     gene            6965..>7257
                     /locus_tag="AC161X_02880"
     CDS             6965..>7257
                     /locus_tag="AC161X_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012378401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HIR4988526.1"
                     /translation="MPNVVRAAVVQTEWTGDKDSMIKKNADYAREAAQQGAKVMCFQE
                     LFYGPYFCQVQENEHFEYAEPIPDGPTTKLMQDLAKETGMVLVVPIFEKEQEG"
BASE COUNT         1286 a         2199 c         2312 g         1460 t
ORIGIN      
        1 aagaagggct cagccgccgt tctcgggttc tttgaatcat tggtgtgggt tctcgccatc
       61 gccagggtcc tcagtgggtt cgacgacccg gcccgcgtgc tcgcctttgc cctcggattc
      121 gcagcaggca cctacggtgg tgcgctggtc gaagagtggc tggcgatcgg ccaatccctg
      181 ttgcgtgtga tcgccccggt ggaaacgccg agtgtcgccg agttgctacg cgcccgcggt
      241 cacggcgcca cagtggtcaa cggtgacggg cgcgagggcg aggtccggat cactttttgt
      301 gtggttcctc gccgcaaggt ttcggagatt gtcgacttga tcgagacgac gaaccccgat
      361 gccttcatca cggtggaaca aacgacgacc gtccgcctgc tccaacgcca ccaatagccg
      421 ggcccggccg gagcaggtca gccttcgacg accagcgttc ccgtcatctg ggaatggagc
      481 ccgcagaagt aggcccaggt acccggttcc agggtgatct caaccgttgc cccaccattg
      541 agattcatcg gatcgaggcc cgccggctgc ccgtcatggg tgtcgaagac gatctggtga
      601 atcaccagca tctcgttctt ccaggccacg gatttgcccg cagggatggt cacctcagca
      661 gggaaggaga gactgtcgac cgtgatcagc acctgatcac tcggagccgt ggtcccggtg
      721 gcggccgttg tggtcgtggc ctcggagccg ccgccacaag cggcgaggaa gagggccagg
      781 gcagtcatag cgagggtcag tttgcgcaat cgattccttc cttgttgccg ggacaatacg
      841 acttaattcg gcgtttggcc tgccaagagc ttgtctaagg gcgcagggaa tcgagcgctc
      901 acagtggtgc cgtcgagccc ggtctgcaga ctcccggcct cgcgctcgat cgcgttagac
      961 gagtccgttc cgacgtcttg gaggagtcga tatctgacct cgccgccggg gcgttgtgac
     1021 catcctccga cgattcgccc gtcacaccac acggtggctc cggcgtttcc gtttcgatcg
     1081 aagacccggg gagcatggtc gcccagatac caacctcgtt ccttccagcc catggtcgtt
     1141 gggtcgagcg aaggcaggaa cgcgacccac gaggtcgagg ccgtgaccgg ctcgaggtcc
     1201 tgggggtgga ggtaggcggg accgatgtcc gtttcgacct ctgccgcctt gagattccga
     1261 agcgcgatcc ggacatcgcc tttggtccac ccggtccacc atgccatgtc gacttcggtg
     1321 atcggcccga agcccagtaa caatcgtccg aggatctcga cctgggccgc gcccttcggc
     1381 atggcgggga tcggtgctcc cagccaatcg gctgtttccg cccaccggta ttggccggat
     1441 acccagctgc ctcggggcct ggctctaatc acgcgtccct ccgcggccag tgcgaagagg
     1501 acccgggtcg atgctcccgt gcggccgatc accgtcccat cggccttgta gaaggtgaac
     1561 tgctcccgga gctccggaac gtccttcgtc aattcgaccg ccgccatggc ttcgcctctg
     1621 gcctcgatga cggcgagcgt cttctccgac accttgcgat accagtcggc accgtcggcg
     1681 gagagccctg ctccttcgac catgttcgcc aggcgtctca actctgggcc ggcgatcttg
     1741 atcgtggacg agttgtggac ctcggtgacg gattccgtcg gtaccaccca gagggttcgc
     1801 ctcatgccca gtacccgggc cagggaccgg ttttcgtaga ggtccgattc gaggtccgag
     1861 accttcaggg acgtcacccg tgcccgggag gacagaaaca ccgtggcagg gtctgaggag
     1921 tgaagcccga ccatgtcaga gaccacagcg acggtgctct ctgactgctc ggcgaggtgg
     1981 tgacggatcc ccagtcgggc ccgcctctca tcgacaccga ccattctcat tgcggtccga
     2041 ggttaggacc actgcccggg gaggtcaggc ggggacgatc ccgacccgtc gctcgaaatt
     2101 ctctgctcgc tccatgatct gtgcgggcag ggccatgatc ttgtcttgca tttcggcggc
     2161 ggcggttgtg agacctgtca ggtcgccgat cgaccagtcc ttcagcagag gcaccagcac
     2221 tcggtcgtgg tggattcgca ggttgtagac gccggcacgt gccacgtcga tcgatctgcg
     2281 gacgaatcgg ggcatagcgg cgccgggcat ctggaagttg tcgaacactt tgaagatccc
     2341 acccaggacc agggaaggct cggagtcgag cattgcctgc atcaccgact tgtagaaaat
     2401 gaagtggtgg ttctcatcag aggcgatgag ccgcatcaat tcgaaggccg cagggtcgtc
     2461 ggtgatcttg cccgcgttgc gatggctcac ccgggtcgcc aactcctgtg ccgcggtgta
     2521 ggcgaagatc tcggtcgggt cgtcaaaggt gggttggtac ccgttgatca tcgtcgactt
     2581 gcggtcgtcc tccagctcgt aggggtcgca gttgcgtgag gtgagcaagt agctgcggat
     2641 agcgatggca tgttgcgcct cttctgcggt ccacagatga ttccaggcgc tgaacgccga
     2701 ggtcggcggc atgtatttct cgatcagggc gtggtaatag ggaaggttgt cttcggtcag
     2761 cagattcagg acgagtgcgg ttcgtgcgtt ctcggagatg gtgcactgcg agacgtccca
     2821 gggcttctcc ttgaagctct caccgcgttc ccaggggaca aattcgtgga agtaccaatc
     2881 actccgccgc tgctgatgtt ctttgatcag tcggtcgacg accttgtcga ctgcctcgac
     2941 cagttcatat ttctcatctc gagccattca tcgcctcatc gtgtgtggtg gtaacggtac
     3001 cgacttggag ttgcttgcca aacatcgggg tcttaggcct agacccatgt cagagccgtg
     3061 cggttacacc cgccggtaga gtcccgcccc gatgaccacg agaggcgtaa gtcgtgcaac
     3121 ccgcagtggt ctctcgattc tcgggatcct tgtggtgttc gtgctcctct gggagggcta
     3181 caagttcatc tggacggctc tggaatggac ctggccagtc cggcccgaca accgcacgat
     3241 gccccatacc tgggacatca tcggcgcgtt gttccagccg gcgcagcgaa acgggccact
     3301 cttactctgg gtggagctca aggcagccct gctcactctc cgcgaggcgt ttgtcgggtt
     3361 cctgatcggg ggggcgatcg gtttcgggct gggcgtcttg ttcgtcaggt ccaccactgc
     3421 tgagcgggcg ttcatgcctt ttgtggtggc ctcacaaacc gtccccatca ttgcgatagc
     3481 cccgatggtc gtgatttggg gcgggaggtt gtcagtgccg caatggctga cggtctcggt
     3541 gatcgccgcc tatctgacct ttttcccggt agtcatcaac accgtccgag ggctgcggtc
     3601 gccggagcca acctcgatgg aactgatgcg gtcctacgcg agcacgccac gtgagactct
     3661 gtggaaggtc caggttccct cggctctccc ttacatcttc accgcactca aagtctcggc
     3721 tgcggcgagt gtgatcggcg ccattgtggg ggagcttccc tcaggattcc ggggagggct
     3781 tgggcgcgac ctgcttcagt tttcccagca attcgcggcc gcgccccaga agttgtttgc
     3841 ggctgttgtg ctcgccggtc tcgccgggat tgccttcgtt ggcctgatca cgctgactga
     3901 gcatcgtttg gtggctgcat ccaggagaca gtcctgatgc cgagcgcggt tgaagcacga
     3961 ggtgtcggga tggtcttcgg acagggcgcc gaatccatcg aagcgctgaa gaacatcaat
     4021 ttgacgctgt ctgcgggtga tttcgcctcg ttgatcggcc cctccggatg tggcaagtcg
     4081 accctgctgc gattgatcgg cgatttggca accccatcgg ttggagagct ggcaatcaat
     4141 ggcaagacac cggaacaggc tcggctggat cgtgactacg gcatggtgtt ccaatcggca
     4201 accctcctcg attggcgcac agtcgaaaag aacgtcgaac ttccgttgga gatcatgggt
     4261 ttcgacagcg ctgctcgtgc cgccaaggca caggaaatgc tctccctggt tcagctcgag
     4321 cagtttgccg accactatcc atggcagctg tcgggcggga tgcagcagag ggtcgctatc
     4381 gcccgggcac tggctttcaa cccctcccta ctcctcatgg acgagccgtt cggggcactg
     4441 gatgaaatga cccgcgagtt gatgcaacag gaagtcctga ggatctggga ggagacggag
     4501 accaccgtga tcttcgtcac ccattcgatc ccggaagccg tcttcctgtc gactcgtgtc
     4561 ctggtcatgt ctcctcgccc cggctcgatc agcgacgaga tctccatcga cctcgccacc
     4621 aaacgggacg aagacaccag ggaggccact cgctacttcc agctgatcac cgaggtcagg
     4681 gaagccctgc gccgtggaga gcgaggtgat gggtgaccga gcgtgacgaa gtcattcggg
     4741 agagccacga gacgtcagtg atcacctcgt tcgagggagg cgggcctgta tcgacatcca
     4801 gacggtcggg gctacggcgc tttggcgctc ggtacgggcc ggcggtcctg gcgttctttg
     4861 ctgtgatcct ggtttgggag gggcttaccc ggctgttcag cgttcagagc ttcatcctcc
     4921 ccaagccgag cgagatcatg gtgagcttct tcgagacctc ggcgacgatc tggggtgtcg
     4981 gcttcaacac gctcatcgag gcggtcagcg gctttctgct cggcacgatc ctggcggtag
     5041 ccacctccct ggccgccgct cgatgggtcg ggttccggga gggcatgctc cccatggcaa
     5101 tcgctctcaa cgccactccg atcgcggcgc tggcaccaat cttcaacaac tggttcggtc
     5161 tcaccaaccc cttttcaaag tcaggcgtcg tcgccgtcgt tgtctatttc ccggtgatga
     5221 tcaataccac caggggattg ctcgaggtcg acccagccga attggaactg atgcgctccg
     5281 tggcagccag tcccgtcgag gtgatgcggc gggttcggat ccccaacgcc ctgccctatt
     5341 tcttcgcctc gatgaaggtg gcgacggcgc tctcgctgat cgcggcgatc attgccgagt
     5401 acttcggtgg accacaggac gttctcgggc agtacatcgt gaatcgcgcc cagctattcc
     5461 agttccccga cgcctgggcg gcgatcctcg ttgcttcgct gatcggggtg acgttctatg
     5521 cgttgatcct cctggccgag cggatcttca tgccatggca tgtctcgatg cgtcgctcct
     5581 gaccggtccc gatttcgccg tacatcgccc cctcgcgacc ggataacgtt cccggcctca
     5641 actaggagga gaaacttggc aacgaccaaa tggcgccacc acgtcctgtg gctggcggcg
     5701 ctcgcaattg tcctcgccgc ttgcggtgac accggtaccg gctcaacgac gaccgaggga
     5761 acagcagagg gaaccagcac cacggccgga acgggtacca ccgcggcgcc cggcaccacg
     5821 gtcgcacctg acgagtgcac ggaaccggat ccggttcggc tgcagcttca atggttcgct
     5881 cagtcccaat ttgccgggta ttacgtcgca ctcgacaagg gcttctatga ggctcagtgc
     5941 ctcgacgtga cgattctcga aggcgcggtc gagatcgtgc ctcagcaggt gctcgccacg
     6001 ggcggcgccg agttcgggct ggcgtgggtt cccaaggctt tggtgtctcg tgagggaggc
     6061 gccgacatcg tcaacatcgc ccaggtcttc gagcgttccg ggacgctcca ggtctcgtgg
     6121 gccgactccg gaatcaccga gccggctgac tgggcaggca agaaggtcgg caactgggga
     6181 ttcggcaacg agttcgagtt gaccgcggcg atcagcaagt tcggtgtcga agacgtcgag
     6241 ttggttggtc aggacttcac catgactgcc ctgctcaaca gggaaatcga cgcagctgag
     6301 gccatgatct acaacgagta cgcccaggtg ctggaagcgg tcaatcccga caccggtgag
     6361 ctctatcagc cggaagacct caatgtgatc agttacaacg aggttgggac ggcgatgctc
     6421 caggacgcgg tgtgggtcac ctccgactgg atcgccgacg aggcgaacca ggacatcgcc
     6481 acccgtttcc tgaccgcttc gttccagggt tggatccact gtctcgacaa cttcgacgaa
     6541 tgcgtccagg tggttttgga caacgggtcg acgctcggcc agagccatca ggaatggcag
     6601 ctgaacgaga tactcggatt gatcttcccg gccaccaacg gcatcggcgt gatgaacacc
     6661 gagttgtggg accagacggt cgccgtggcc accgagcaga ttccggagct gcagggggtt
     6721 gagatctcgg ccgacgccta tcggaccgac ctggccgagg ccgcaatcgc cgctttgggc
     6781 gatgccgaca ccagcggcgc tggcttcacg cggcgtgagg tcgagttgag ggagaacggc
     6841 gagtagcgaa cgaagagagc ggtttctccc ctcagcgaac gaagtgagcg ttggggggag
     6901 agccggcgaa gccgggaggg gaggggtccc ccgccgcgct aggtttgcca caaaggagac
     6961 ctgaatgccc aacgtcgtga gagccgccgt cgtccagacc gagtggacgg gggacaaaga
     7021 ctcgatgatc aagaagaacg ccgactatgc ccgtgaggcg gcgcagcagg gagccaaggt
     7081 gatgtgcttc caggagctct tctatgggcc ctacttctgc caggtgcagg agaacgagca
     7141 cttcgagtac gccgagccga tcccggatgg ccccaccacc aagctgatgc aggacttggc
     7201 caaagagacc gggatggtct tggtggtgcc catcttcgag aaggagcagg aaggctt
//
LOCUS       DBNHXU010000110         2809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_432714, whole genome shotgun sequence.
ACCESSION   DBNHXU010000110 DBNHXU010000000
VERSION     DBNHXU010000110.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2809
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_432714"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /gene="rpiB"
                     /locus_tag="AC161X_02885"
     CDS             <1..434
                     /gene="rpiB"
                     /locus_tag="AC161X_02885"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019014499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribose 5-phosphate isomerase B"
                     /protein_id="HIR4988527.1"
                     /translation="ALGSDHAGYSMKVHLASWLAESGHAVYDLGTHSAEPVDYPDYAA
                     AVGQAVLDGRADRGIIICGSGAGASVAANKLNGIRAAVAHDAYTAHQMVEHDDVNVIC
                     LGSRVIGLSLALDLVTTFLEAKFSREERHVRRLEKIRSLET"
     gene            complement(437..1093)
                     /locus_tag="AC161X_02890"
     CDS             complement(437..1093)
                     /locus_tag="AC161X_02890"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR4988528.1"
                     /translation="MTQRTSKKPPAKPTRNLSTLWWIIGGVVGLALIVLLAISIAGEE
                     DIDTTVGYGEVTVDGDPLPTFSDPSNDPAVGMTLATVSGGDWDGNQYTIAPDGTPKIV
                     LLLAHWCPHCQAEVPVVQQWVNAGNLPDGVDMYSLTVFTDPLRGNWPPQEWLEDAGWT
                     TPVIMDDDAGTAAVTFGMASTPMYVVLDGDNKVVQRVSGEIGTDGLDALSLIALQTAG
                     "
     gene            complement(1090..1623)
                     /locus_tag="AC161X_02895"
     CDS             complement(1090..1623)
                     /locus_tag="AC161X_02895"
                     /inference="COORDINATES: protein motif:HMM:NF014639.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HIR4988529.1"
                     /translation="MSVEATLVYNNVLAILSLVALVGAVGLLIFRMVKGPEAAGLLGD
                     NAIWLAWIVALVCTVGSLIYSEVIHFVPCRLCWLQRIAMYPLAFILLVGAIRREAVVK
                     YYALPLALTGLAVSIYHNLIQLNPSLEGGSCDPLNPCSARTIEVFGFIDLPFMAGAAF
                     ILISVLLAFYVRTENYE"
     gene            complement(1642..1714)
                     /locus_tag="AC161X_02900"
     tRNA            complement(1642..1714)
                     /locus_tag="AC161X_02900"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1678..1680),aa:Glu,seq:ttc)
     gene            complement(1844..>2809)
                     /locus_tag="AC161X_02905"
     CDS             complement(1844..>2809)
                     /locus_tag="AC161X_02905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988530.1"
                     /translation="FYARETNQGWAMSVNGHLWSPTSMPLGGGFPDRLASEGMKQAVF
                     EVNGETWAMVSEEGEAPGWLLHRQGYEWVEAEFTALEAPDGLGFVGGIRGVSLLNPHA
                     YDALNTTVVIHNGAMMTVSVPDSNATEMVMMGSVVYALSVHTVVGISRELQLWKTSDG
                     VEWSRVAGPRLQDEKWTSVHLTSGHGRLMLSLGSYSDPRSQTIWTTDDGEEWDQLEVF
                     LGSEPDADVGRGGGIDPAPAQSTEFGWMVTSLGEKWSSGLYDPDNFRLLISADGLEWE
                     KVSVPGKPGGLVAGPPMPLAYQAGWFVRDVTQLSRDYTLVGSFLD"
BASE COUNT          570 a          892 c          845 g          502 t
ORIGIN      
        1 tcgcactcgg ctccgaccac gccggatatt cgatgaaggt acacctggca tcctggctcg
       61 ccgagtcggg ccatgccgtc tacgacctcg gcacccactc tgcggagccg gtcgactatc
      121 ccgactacgc ggccgccgtg ggccaggccg tcctcgacgg gcgggccgat cgtggcatca
      181 tcatttgcgg ctcaggcgcc ggagcatcgg tcgcagcaaa caaactcaac ggaatccggg
      241 cagccgtggc ccacgacgca tacaccgctc accagatggt cgaacacgac gatgtcaacg
      301 tcatctgcct tggttccagg gtgatagggc tctcgctggc cttggacctg gtcacgacct
      361 tcctcgaagc aaagttcagc cgcgaggagc gccatgttcg gcgtttggag aagatccggt
      421 cgctagagac ctgatctcag ccggcagttt gcagcgcaat cagagacaac gcgtcgagac
      481 catctgtccc gatctcacct gagacccgct gcaccacctt gttatccccg tccagtacca
      541 catacatcgg ggtgctggcc atcccgaagg tcaccgcggc agtgccagcg tcgtcgtcca
      601 tgatcacggg cgtggtccat cccgcgtcct ccaaccactc ttgaggcggc cagttgccgc
      661 gaagcgggtc ggtgaacacc gtcagcgagt acatatcgac cccatcgggc aggtttccgg
      721 cattgaccca ttgttggaca acggggacct cagcctggca atgagggcac cagtgggcga
      781 gcaacaagac gatcttgggt gtcccgtccg gagcgatggt gtactggttg ccgtcccagt
      841 cgccgccact gacagtcgcc aaggtcatcc cgacagccgg atcattcgac ggatcgctga
      901 aggtggggag cgggtcacca tcgacggtta cctccccgta accgacggtg gtgtcgatgt
      961 cctcttcgcc cgcgatcgaa atcgccagca ggacgatcag ggccagcccg acgactccac
     1021 cgatgatcca ccacagggtg ctcaggttgc gagtcggctt ggccgggggc ttcttcgagg
     1081 tgcgttgggt cattcgtagt tctcagttct cacatagaag gcgagcaaga cggatatcaa
     1141 gataaacgcg gcgccggcca taaacggaag gtcgatgaag ccgaacacct cgatcgttcg
     1201 cgcagaacaa gggttgagcg ggtcgcagga gccgccttcc aggctcggat tgagctggat
     1261 caggttgtga tagatcgaca ccgctagccc ggtgagggcg agcgggaggg cgtagtactt
     1321 gaccaccgcc tccctgcgga tcgcaccaac caggaggatg aaagccagcg ggtacatggc
     1381 gattcgttgc aaccagcaga ggcggcaggg gacgaagtga atcacctcgg agtagatcaa
     1441 cgagcccacg gtgcaaacca gggcgacgat ccacgccagc cagatcgcat tgtctcccag
     1501 aagaccggcc gcttcgggtc ccttcaccat ccgaaagatg aggaggccga ccgcgccgac
     1561 taacgccacc agggagagga tggctaagac gttgttgtag acaagggtgg cttcgaccga
     1621 catcacgctt ctctcggtgg gcagtcccga ccggattcga accggcgttt ccggcttgaa
     1681 aggccggcgt cctgggcctc tggacgacgg gacctgggga accaaggaga ggttaatggg
     1741 tcgcggatcg gtatccccca cccgtaatag ggatcgacag ggttcagatc cggatctgta
     1801 gatccgaatt acggagtgtt gggacgccag ttgccgccat gtctcagtcg aggaatgagc
     1861 ccaccagggt gtaatccctt gacaactgtg tgacatcccg cacgaaccac cctgcctggt
     1921 aggcgagcgg catcgggggg ccggccacaa ggccaccggg ctttccgggg acacttacct
     1981 tttcccactc caaaccgtcg gccgagatca gcagacggaa gttgtccggg tcgtagagac
     2041 cggaactcca tttctcgccg agagacgtca ccatccagcc gaactcggtc gactgtgccg
     2101 gagccgggtc aatgcctcca ccccgaccaa catcagcatc tggctcggag cccaagaaga
     2161 cctcgagctg atcccattcc tccccatcgt ctgtggtcca gatggtttgt gatcgggggt
     2221 cactgtatga gccaagggag agcatgagcc gaccgtgacc cgaggtcaga tgaaccgacg
     2281 tccatttctc gtcttgcagc cgcgggcccg cgaccctgga ccattccacg ccgtcggagg
     2341 tcttccagag ctgcaattcg cgtgaaatgc ccacgaccgt gtgcaccgaa agtgcgtaga
     2401 cgaccgatcc catcatcacc atctctgtgg cgttcgagtc cgggaccgac accgtcatca
     2461 ttgcaccgtt gtgtatgacc acggtggtgt tgagtgcgtc gtatgcgtgg gggttcagga
     2521 gagagacgcc gcgaatccca ccgacgaagc ccaagccgtc cggggcctcg agagcggtga
     2581 actcggcctc gacccactcg tacccttgcc ggtgcaagag ccaccccgga gcctcgcctt
     2641 cctccgacac catggcccag gtctccccgt tgacttcgaa tacagcctgc ttcatgccct
     2701 cgctggcgag ccgatctggg aacccacctc ccaatggcat cgacgtcggt gaccacaaat
     2761 ggccgttcac cgacatggcc cagccctgat ttgtttcccg ggcgtagaa
//
LOCUS       DBNHXU010000111         1698 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3408845, whole genome shotgun sequence.
ACCESSION   DBNHXU010000111 DBNHXU010000000
VERSION     DBNHXU010000111.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1698)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1698
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3408845"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..457)
                     /locus_tag="AC161X_02910"
     CDS             complement(<1..457)
                     /locus_tag="AC161X_02910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988531.1"
                     /translation="MGSDDTRSEYLATGSGEKPADHDQLDLIRGVLEGNSTWAEPPGG
                     VMEGMLATISRDGDAPTQVSKPGAWGRRPWLVATAAVAVISLLVVGVVAAFDDPDLKT
                     VVAVNGTDLAPAATGIATLRPTPSGWYIRLDVSGLPAAPEGSYYEGWVWS"
     gene            complement(503..1075)
                     /locus_tag="AC161X_02915"
     CDS             complement(503..1075)
                     /locus_tag="AC161X_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4988532.1"
                     /translation="MRNTHQGGAWFATWHNSEMDPEVLQRFRIGDESAVKAVYDRFGG
                     PVFALSMSILGEAGLAADATQQTFIKAWRAASTYDPDRSFSPWIYAIARRTAIDIYRK
                     RARTVVSDQVDVATISPGLETVWEVFEVRAALDQLPDDERQVVKLSHFDGFTHAEIAD
                     QLGIPVGTVKSRSHRAHQRLLGLLRHLEEM"
     gene            1097..1591
                     /locus_tag="AC161X_02920"
     CDS             1097..1591
                     /locus_tag="AC161X_02920"
                     /inference="COORDINATES: protein motif:HMM:NF015596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COG4315 family predicted lipoprotein"
                     /protein_id="HIR4988533.1"
                     /translation="MKRQLFPLLLAGVLILTACGDSGGDGSGGTDAAGDSISTASTDL
                     GTVLVDPEGFTVYVFLNDTNGESTCYDSCAATWPAVPGDTAISSDLDDSIFGTTTRTD
                     GTEQLTVDGQPLYRYAPDANPGDTAGQGVGEVWFVVGSDGAMIGGPEAAATTGGGKYG
                     AGGD"
BASE COUNT          324 a          559 c          520 g          295 t
ORIGIN      
        1 cactccacac ccacccttcg taatagctgc cctcaggtgc ggcgggtagc ccgctcacgt
       61 cgagacggat gtaccagccc gacggagttg gtcggagagt ggcgatacca gtggcggcag
      121 gcgccagatc ggtcccgttg acggccacca cggtcttgag gtcaggatcg tcgaacgccg
      181 caaccacccc gacaacgagc aagctgatga ccgcaaccgc ggccgtcgca accaaccaag
      241 gtcgtcgccc ccatgccccg ggctttgaga cctgtgtggg ggcatcacca tctctgctga
      301 tggtggcaag catgccctcc atgacaccac ccggcggttc agcccaggtc gagttgccct
      361 cgaggacgcc acgaatcagg tcgagctgat catggtcagc cggcttctct cccgaccccg
      421 tggccaggta ctcggagcgc gtgtcgtcag atcccatcac acccaacctc tacgacctcg
      481 atcggccctg cggttcgcgt cctcacatct cctccaggtg tcggagaagc ccgagaagcc
      541 tttgatgggc ccgatgtgac ctcgacttga ccgtccccac cgggatcccg agttggtctg
      601 cgatctcggc gtgggtgaag ccgtcgaaat gggagagctt gaccacctgt cgctcatcat
      661 cgggaagttg gtcgagcgca gctcgcacct cgaacacctc ccaaaccgtc tccaagcccg
      721 gcgaaatggt cgcgacatcg acctggtcac tgacgacggt gcgggcccgt ttgcgataga
      781 tgtcgatggc ggttcgtcgg gcaatcgcat aaatccaggg gctgaagctc cgatccgggt
      841 cataggtgga cgccgcccgc catgccttga tgaaggtctg ctgggtggcg tctgcagcga
      901 gaccagcctc gccgaggatg ctcatcgaaa gggcgaacac cggcccgccg aagcggtcat
      961 agaccgcctt gacggctgac tcgtccccga tccggaaccg ttggagcacc tcaggatcca
     1021 tctcggaatt gtgccaggtc gcgaaccatg cgccgccctg gtgcgtattg cgcacacgac
     1081 gatctggaga aatcagatga aacgacaact cttcccgctc ttgctcgccg gcgttctgat
     1141 tctcactgcg tgcggggaca gcggtggtga tggaagcggc ggcaccgatg cggccggcga
     1201 cagcatttcc acggcgtcaa ccgacctcgg gacggtcctc gtcgatcccg aaggattcac
     1261 cgtctatgtc ttcctcaacg acaccaacgg cgagagcacg tgttacgact cgtgcgccgc
     1321 gacatggccg gcggttccag gtgacaccgc tatcagctcg gatctcgatg actcgatatt
     1381 tggcaccaca acccgcaccg acggaaccga gcaactgaca gtcgacggcc agcccctcta
     1441 tcgctatgcg cccgacgcca atccgggcga cacggccgga cagggagttg gcgaggtttg
     1501 gtttgtcgtt ggatcggacg gggcgatgat cggcgggccg gaggcagcag cgaccaccgg
     1561 cggaggcaaa tacggcgcag gcggcgactg agcctggttt tgcctgatgg gtgtgtcctc
     1621 gggaccacag acccggggac acccccgtca cttgtaacgg gccgtttgga ccagatcgaa
     1681 ccagtcctca gcgcccaa
//
LOCUS       DBNHXU010000112         7574 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1822532, whole genome shotgun sequence.
ACCESSION   DBNHXU010000112 DBNHXU010000000
VERSION     DBNHXU010000112.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7574)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7574)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7574
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1822532"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="AC161X_02925"
     CDS             complement(<1..473)
                     /locus_tag="AC161X_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR4988534.1"
                     /translation="MEHVQVTIDGAVALVRLDRPPVNALSEQVATELKQAFVECADPE
                     IRAVVVTGQPHFAAGADIKGFQAAFEAGTKERTAWTLQEAIAALEGLEKPTIAAIHGY
                     ALGGGLELAMGSDFRYLAEDALVGQPEVLLGIIPGAGGTQRLGRLVGYQRAKEMIL"
     gene            complement(557..3280)
                     /locus_tag="AC161X_02930"
     CDS             complement(557..3280)
                     /locus_tag="AC161X_02930"
                     /EC_number="2.3.1.-"
                     /EC_number="6.2.1.13"
                     /inference="COORDINATES: protein motif:HMM:NF024938.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0043758 - acetate-CoA ligase (ADP-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0046356 - acetyl-CoA catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetate--CoA ligase family
                     protein/GNAT family N-acetyltransferase"
                     /protein_id="HIR4988535.1"
                     /translation="MAEYPSEYELDIVLRDGGGARVRPIKPEDAGLIRDFFETLGPES
                     RYFRFFRIKETLEPKEVEYFTQVDYADRMALIALFEGRMIAVARYDREDHDADSAEVA
                     FAVADKHQGRGIGTQLLQLLTNHARSHGIERFHAYVLPENRQMMRLFRNSGYEMTRTI
                     DDGVFTVDFPVADSEGVHEAEAEREKRAVAASLLPLFFPRSVAVIGASSDPSSIGGKL
                     FHNILSTGFTGPLYPVNPKAKVINSVRAYPTILDVPDEVDLAYIVVPQRFVIDVASQC
                     AEAGVRGIVVISAGFSEIGPEGAILEHELLEIVRNSGMRMVGPNCMGLLNTASSVQLN
                     GTFAPVYPPAGNVAMSSQSGALGIAILDYATNSNIGISQFVSVGNKADVSGNDLILAW
                     EDDPQTDVITLYLESFGNPKRFSRIARRIGRRKPIIAVKSGRSSAGSRAASSHTGALA
                     SSDVAVQALFRQAGVIRVDTIEELFSAANLLANQPIPKGNRVGIVTNAGGPGILAADA
                     LEANGLTLPELSAELRAEIGARLPEEASTRNPVDLIASGGPAEFTHAVTLLLDSGEVD
                     SVMVIYVPTSSEGAQEVAVALSGCQQRYEGPVALLSVFMHSGDTARHLAGGEGRRAIP
                     TYTFPEAAALALARAVAHGVWRLRDPGLEARLEPEAEVAIRAIIDDALIGVSATGAWM
                     EPEQVDAVLTAAGLRVPKSAVAHSAEEAAASAGAIGGSVVVKVISETALHKSDVGGVV
                     LGVEGDADVREAYDTVTGAVFDADGVLVQEFVGGGHETLIGMTQDPNFGPLVGFGLGG
                     VYVELLQDVAFRIHPLTDVDAAEMIRDTKGFRLLQGYRNTAEGDVAELEKALLRVSGL
                     ISAVPELVEMDLNPVKVLPPGEGVVVVDARMRIQPVPDKRHPRMRDLPGVR"
     gene            3530..3811
                     /locus_tag="AC161X_02935"
     CDS             3530..3811
                     /locus_tag="AC161X_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988536.1"
                     /translation="MDRFNLFLSIRWIATVLAVLLVIHLIAVWMERRGWIYYRKTGRR
                     SYGVAVSNAMAEFDAVLNPASEHRVIEERHHEAMRYVIDISDVEDDPID"
     gene            complement(3824..3908)
                     /locus_tag="AC161X_02940"
     tRNA            complement(3824..3908)
                     /locus_tag="AC161X_02940"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3874..3876),aa:Leu,seq:taa)
     gene            complement(3921..4805)
                     /locus_tag="AC161X_02945"
     CDS             complement(3921..4805)
                     /locus_tag="AC161X_02945"
                     /inference="COORDINATES: protein motif:HMM:NF014587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HIR4988537.1"
                     /translation="MGGNAMNVGVVDIGTNSIRLLITGGVREVRLEAVTGLGTGVDST
                     GVLSESRIVETLVVMNRYGRLMDEAGVEVRKAIATSASRDADNRESFFDRVEEMIDIR
                     PTLISGEEEARYAYQGAVQDFGGPGPVVVSDIGGGSTEFVTETSGISIDIGSVRITER
                     AVPYRPASREEIVEARALIKELFSQVPAIEFTTLIGVAGSWTSLASVARALPLGTSVH
                     HREVSRVEVGRIIDVMGLLTVEETADLPGLDPKRAPVILGGILVAEGVMDALGADHAL
                     VSEHDTLDGVAMALLALP"
     gene            complement(4787..5305)
                     /locus_tag="AC161X_02950"
     CDS             complement(4787..5305)
                     /locus_tag="AC161X_02950"
                     /inference="COORDINATES: protein motif:HMM:NF016314.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF501 domain-containing protein"
                     /protein_id="HIR4988538.1"
                     /translation="MSRLDDREVIETQIGRPLRADSKAVSRCHLDLPVVVEVPPILDD
                     GTPFPTLYWLTCPLATTRIGRLEGSGGVKRMEQKAAADPEFGRRLDEAHASYATKRDA
                     MVPEGASPVPSGGVGGSTVGVKCLHAHYAHTRSGDSNPVGELVASWIEPLDCQIPCVA
                     DGAMNPAWVAMP"
     gene            complement(5302..5697)
                     /locus_tag="AC161X_02955"
     CDS             complement(5302..5697)
                     /locus_tag="AC161X_02955"
                     /inference="COORDINATES: protein motif:HMM:NF016839.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsB family cell division protein"
                     /protein_id="HIR4988539.1"
                     /translation="MKMRSRSGVALVTLLFILMGAAFLTQVVPYRQILDSRRQVQAAH
                     EQLASLEAQNEVLSADVEALSTPEEIERLARDKLGYVREGETAYVVLDPPGAETEKTT
                     VSDIPPPPDKTWVDRVWDFITGEDIHSSG"
     gene            complement(5694..6974)
                     /gene="eno"
                     /locus_tag="AC161X_02960"
     CDS             complement(5694..6974)
                     /gene="eno"
                     /locus_tag="AC161X_02960"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011639733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000015 - phosphopyruvate hydratase
                     complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HIR4988540.1"
                     /translation="MTHTTIERIHARQVLDSRGNPTVEAEVHTAGGGFGRAIVPSGAS
                     TGAMEAVELRDGGDRWGGKGVTRAVANIRDVLAPALIGADATGQEHIDQVMLDLDATT
                     NKSNLGANSILAVSMAVSRAAAASLDQPLYRYLGGPAARTLPVPCLNVLNGGAHAANS
                     VDIQEFMIVPGGLPSFSEALRAGTEVYQSLKKVIGGRGLGTNVGDEGGFAPDLPTNES
                     ALELLVEAIDVAGYKLGDEIALAMDVAATEIHRSGFYELDGRNLSSEDMVAYLVDLVD
                     RYPLVSVEDGMAEDDWDGWKELTNQIGRRVQLVGDDLFVTNEDFLRRGIAEGAANAIL
                     IKVNQIGSVSETLHTMATARTASYGMMVSHRSGETEDSYISHLAVATNAGQIKSGAPA
                     RGERTAKYNQLLRIEEGLGSEARYAGWSTFRRPR"
     gene            complement(7047..>7574)
                     /locus_tag="AC161X_02965"
     CDS             complement(7047..>7574)
                     /locus_tag="AC161X_02965"
                     /inference="COORDINATES: protein motif:HMM:TIGR00444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG family protein"
                     /protein_id="HIR4988541.1"
                     /translation="AGAFNIDDVAEVMRQKLVRRHPHVFGDVEVGSAEEVKSNWDRIK
                     EEERGSARESALDGVPSGVPALQRASKIQNRAAKVGFDWDEALQVLPKVREELGELEE
                     VLGDPTRAREELGDVLFSVVNLARHLGLDPEIALGQATDRFEARFRRMEAEGPLDGLD
                     LDGLNERWEQSKEED"
BASE COUNT         1372 a         2507 c         2350 g         1345 t
ORIGIN      
        1 aggatcatct ccttggctcg ttggtagcca acgaggcgac ccagccgctg ggtccccccg
       61 gcaccgggga tgatcccgag caggacctcg ggctggccga ccagggcatc ctcggcgagg
      121 tagcgaaagt ctgaccccat cgccagttcc agaccaccgc ctagggcgta cccgtgaatt
      181 gcggcgatag tcggcttctc caggccctcc aatgcggcga ttgcctcttg aagggtccag
      241 gcagtccgct ccttggtccc ggcttcgaag gcagcctgga agcccttgat gtcggccccg
      301 gcagcaaaat ggggttggcc ggtgacgaca accgctcgga tttcggggtc ggcacactcc
      361 acgaaggcct gcttcagctc ggttgccacc tgctcggaga gggcattcac cgggggtcgg
      421 tcgagacgga ccaacgccac cgcaccgtca atggtcacct gcacatgttc catgagccga
      481 gggtagaccc ctgtttctcc ccccagtgag cggagcgagc gttgggggga gtaccgccgg
      541 aggcggggag gggggtttat cgcacgccgg gcaggtcgcg catccggggg tgacgcttgt
      601 cagggaccgg ctggatccgc atcctggcgt ccaccaccac caccccctcg ccgggtggca
      661 gcactttcac ggggttgaga tccatctcca ccaactcggg caccgcgctg atcaggccgg
      721 acacccgcaa cagcgctttc tccaattccg ccacatcgcc ctcggcggtg ttgcgataac
      781 cctggagcag ccggaatccc ttggtgtccc ggatcatttc ggcggcatcg acatcggtca
      841 gggggtgaat ccgaaacgcc acgtcctgga gcagctcgac gtagaccccg cccaggccaa
      901 atccgacaag cggtccgaag tttggatcct gagtcatccc gatcagggtc tcatgaccac
      961 cgccgacgaa ctcctggacc agcaccccat ccgcatcgaa caccgccccg gtgacggtgt
     1021 cataggcctc ccgcacatcg gcgtccccct ccacaccgag aacgacccca cccacatctg
     1081 acttgtggag agccgtttcc gatatcacct tgaccaccac cgagccgcca atcgccccgg
     1141 cagacgccgc ggcctcctca gctgaatggg caactgccga cttgggaact ctgagaccgg
     1201 ccgcggtgag caccgcgtcg acctgctccg gctccatcca ggcaccggtc gcggaaactc
     1261 cgatcaaggc gtcgtcgatg atcgcccgga tcgcaacctc ggcctccggt tccaggcggg
     1321 cctccaaacc ggggtcgcgg agacgccaca caccgtgggc caccgcccga gccagggcca
     1381 gcgccgcggc ctcggggaag gtgtaggtgg ggattgcccg ccgcccctcg ccgcctgcga
     1441 ggtggcgggc ggtgtcacca ctgtgcatga agaccgacaa cagggcgacg gggccctcat
     1501 aacgctgctg gcaaccgctg agggcgacgg ccacttcctg ggcgccctcc gacgaggtag
     1561 gcacgtagat gaccatcacc gaatccacct cacccgagtc gaggagcaga gtgaccgcgt
     1621 gtgtgaactc ggccggccct ccggaggcaa tcagatcgac cgggttccgg gtcgaggcct
     1681 cctctgggag ccgagcaccg atttcggcgc gcaactccgc cgagagctcg ggaagggtca
     1741 gtccgttggc ctctagtgcg tcggctgcca ggatcccggg gcccccagca ttggtaacga
     1801 tcccgacccg gtttcccttg gggattggct gattggcgag gaggttggcg gcggagaaca
     1861 actcctcgat ggtgtcgacc cgaatcaccc cggcctgccg aaacagcgcc tgcaccgcca
     1921 cgtctgacga tgccaacgcc ccggtatgcg atgaagcggc ccggcttccg gccgagctcc
     1981 tccccgactt gacggcgatg atcggcttgc gacgaccgat gcggcgggca atccgggaga
     2041 accgcttggg gttgccgaag gactccagat acagagtgat cacatcggtc tgggggtcgt
     2101 cctcccaggc cagaatcagg tcgttgccgc tgacgtcggc cttgttgccc accgacacga
     2161 actgcgagat tccgatgttg ctgttggtgg cgtagtcgag gatggcgatc cccagagcgc
     2221 ccgactggct ggacatggcg acgttgccgg ccggcgggta aaccggtgcg aaggtgccgt
     2281 tgagctggac cgatgaagcg gtgttgagaa ggcccatgca gttcggcccc accattcgca
     2341 tcccggagtt gcgaacgatc tcgagcaact cgtgctcgag gatggcgccc tcggggccta
     2401 tctcggagaa acctgcggag atgacaacga tccccctcac cccagcctcg gcgcactgac
     2461 tggcaacatc gatcacgaac cgctgcggga ccacgatgta ggcgagatcc acctcatcgg
     2521 gaacgtcgag gattgtcgga taggcccgaa ccgagttgat gaccttggcc ttcgggttca
     2581 ccgggtacag cgggccggtg aatccggtcg acaggatgtt gtggaacaac ttgccaccta
     2641 tcgaggaggg atcgctggat gccccgatca ccgccaccga ccggggaaag aacaggggca
     2701 acagtgaggc ggccaccgcc cgtttctcgc gttctgcctc ggcctcgtgc accccttcgc
     2761 tgtcggccac cggaaaatcg acggtgaaca cgccgtcgtc gatggtgcgc gtcatctcgt
     2821 agccggagtt gcgaaagagg cgcatcatct gtcggttctc gggaagcacg taggcgtgga
     2881 acctctcgat gccgtgggac cgggcgtggt tggtcagcag ctgtaggagc tgggtgccga
     2941 tgcccctgcc ctgatgcttg tcggcgacag cgaaggccac ttctgccgag tcggcgtcat
     3001 ggtcctcccg gtcgtagcga gcgaccgcga tcatccgccc ttcgaagagg gcgatgaggg
     3061 ccatccggtc tgcgtagtcg acctgtgtga agtactcgac ctccttgggc tccagcgtct
     3121 ccttgatccg aaagaacctg aaatagcggg attcggggcc aagagtctcg aagaagtcac
     3181 ggatcagccc ggcgtcctca ggcttgatcg ggcgcacccg agccccaccg ccgtcgcgga
     3241 gcacaatgtc caactcgtat tcggatgggt attcggccat ctgcaggtcc tcgacatgac
     3301 gctaaccgag tcgtcggggc cgaattcacg tcccgcttct cgacaccttg tcggattaca
     3361 cttttctcac ggggttgaat ctgtgtgtgg gaaggtggtg catcttggag aaagatccgg
     3421 cctcgttcat cgtggggccc gggcccggtt cctctcccct cccccaccca ccggcccggg
     3481 tcccacgacc tttctgaccg atcaaaagta gaattcgccg cgatccgaca tggaccgctt
     3541 caatctcttc ctttcgattc gctggatagc aaccgttctc gcagtcctgc tcgtgattca
     3601 tctgatcgca gtgtggatgg agcgaagagg ctggatctac taccgaaaga ccgggcgccg
     3661 cagttacggc gtcgcagtct ccaacgccat ggccgaattc gacgcggtgc tcaatccggc
     3721 gtcagagcat cgggttatcg aggagcgtca ccatgaggcc atgaggtacg tgatcgatat
     3781 ctccgacgtc gaggacgacc cgatcgactg acaaccccca aggtgcccgg ggtgggattt
     3841 gaacccacac gagccgattc ggctcaaggg ggtttaagcc cctcgcgtct gcattccgcc
     3901 acccgggctc gggcaattgg tcagggtaaa gccaacaggg ccatggcgac accatcgagg
     3961 gtgtcgtgct cggagaccag ggcgtggtcg gcgcctagtg catccatgac cccttccgcc
     4021 accaggatcc ctcccaggat gaccggagcc cgcttcgggt cgagaccggg aaggtcagcg
     4081 gtctcctcga cggtgagcag ccccatgaca tcgatgatcc tccccacctc cactctgctc
     4141 acttcgcgat ggtgaactga cgtcccgagg ggcagtgccc tggccaccga ggccaggctg
     4201 gtccaactcc cggcgacacc gatcaatgtc gtgaactcga tcgctggcac ttgggagaac
     4261 agctccttaa tcagggcacg cgcctcgacg atctcctcgc gggaagccgg acgataaggg
     4321 accgcccgct cggtgatccg caccgagccg atgtcgatgg aaatcccact cgtctcggtc
     4381 acgaactcgg tgctcccgcc cccgatgtcg gacaccacaa ccggacccgg accgccgaag
     4441 tcttgaaccg ccccctgata ggcgtatcgg gcttcctcct cacccgagat caatgtgggg
     4501 cggatatcga tcatctcctc gacacgatcg aagaacgact cccggttgtc tgcgtccctc
     4561 gatgccgaag tggcgatcgc cttgcggact tcgacacctg cctcatccat cagcctgcca
     4621 tagcgattca tcaccaccag ggtctcgaca atccgcgact cggagaggac cccggtgctg
     4681 tccaccccgg tgcccaggcc ggtgaccgcc tcgagccgca cttcgcgcac accaccggtg
     4741 atcaagaggc gaatcgagtt ggtcccgatg tcgaccaccc cgacgttcat ggcattgcca
     4801 cccacgccgg attcatggca ccgtcagcga cgcatgggat ctgacaatcg aggggctcga
     4861 tccaggaggc gaccaactcg ccgaccgggt tggaatcacc ggaacgggtg tgggcatagt
     4921 gcgcatgcag gcatttcacc ccaacggtgc ttcctcccac cccgccgctc ggaaccggag
     4981 acgccccctc cgggaccatt gcatcccgct tggttgcgta agaggcgtgc gcctcgtcga
     5041 ggcggcggcc gaactcagga tcggccgcag ccttctgttc cattcgcttc acgccgcccg
     5101 agccctcaag acgaccgatc cgggtggtgg ctagcgggca ggtcagccaa tagagcgtgg
     5161 gaaagggtgt tccgtcgtcg aggatcgggg gcacttcgac gaccacgggg agatcgaggt
     5221 gacaccggga cacggccttg gagtcagcgc gcagcggacg cccgatctgg gtctcgatga
     5281 cctcgcggtc atccaaacgg ctcatcctga tgaatgtata tcttcgccgg taatgaagtc
     5341 ccagactcgg tcgacccagg tcttgtccgg gggcggtgga atatcggaaa ccgtggtctt
     5401 ctcggtctcg gcgcctggag gatcgagcac gacataagcg gtctcgcctt cgcgcacgta
     5461 tcccaacttg tcccgggcca gccgttcgat ctcctcgggt gtgctcagtg cctcgacatc
     5521 ggcggacagc acctcgttct gggcctcgag agaagccaat tgctcatggg cggcctgcac
     5581 ctggcgccgc gaatcgagaa tctggcggta ggggaccacc tgagtgagaa aggcggcgcc
     5641 catcaagatg aacagaagag tcaccaacgc caccccggag cggctgcgca tcttcatcgc
     5701 ggccgtcgga aggtagacca gcccgcatac ctggcctctg acccgagacc ctcctcgatg
     5761 cgaagcaact ggttgtactt ggcggtgcgc tcaccccttg caggggctcc ggacttgatc
     5821 tggccggcgt tggttgccac cgcgagatgc gagatatagc tgtcctcggt ctcgccggag
     5881 cgatggctca ccatcatccc gtaggaggca gtcctcgcgg tcgccatggt gtgcagtgtc
     5941 tcggagaccg agccgatctg attcaccttg atcaggatgg cgttcgccgc tccttcggcg
     6001 atgcctctcc gcaggaagtc ctcattggtg acgaacaggt cgtcgcccac aagttgcacc
     6061 cgtcggccga tctggttggt cagctccttc caaccgtccc agtcgtcctc agccatccca
     6121 tcttcgaccg acaccagcgg atagcgatca acgagatcga ccaggtaggc caccatgtcc
     6181 tcggaggaga ggttgcggcc gtccagctcg tagaagccgg atctgtgaat ctcggtggcg
     6241 gcaacgtcca tggcgagtgc gatctcgtcg cccagcttgt agccggcgac atcgatcgcc
     6301 tccaccagca actcgagtgc cgactcgttg gtagggaggt cgggagcaaa cccgccttcg
     6361 tcgccgacgt tggtgccaag accccgtccg ccgatcacct tcttcaaaga ctgatagacc
     6421 tcggtcccgg ctcgtagcgc ctcggagaag gaggggaggc cgccggggac gatcatgaac
     6481 tcctggatgt cgaccgagtt tgcggcatgg gccccgccgt tgaggacgtt gagacagggg
     6541 acggggaggg tccgggcggc cgggccgccc agatagcggt atagcggctg gtcgaggctg
     6601 gccgccgccg ctcgcgatac cgccatcgac acggcaagga tggagttggc gcccaggttg
     6661 gacttgttgg tggtggcgtc gaggtcgagc atcacttggt cgatgtgctc ctggccggtg
     6721 gcatcagctc cgatcagtgc gggggcgagg acatcccgga tgttggccac cgcccgggtg
     6781 acccctttgc ctccccaacg gtcgccccca tctcgaagct cgacggcttc cattgcccca
     6841 gtggaggcac cggaggggac gatcgcccta ccgaaaccgc ctcccgcggt gtgaacttcg
     6901 gcttccaccg tcggattacc ccgggagtcg aggacctgac gtgcgtgtat ccgctcgatg
     6961 gtggtgtggg tcatgaaaga agagatcccc ggtctgctgg agggccaagc taccagcagc
     7021 cggtatttgt tctggtgaac cgcggctcag tcctcttcct tggactgctc ccagcgctcg
     7081 ttgagcccgt cgaggtcgag accgtctagt gggccttcgg cctccatgcg gcggaatcgg
     7141 gcttcgaagc gatcagttgc ctgaccgagt gcgatctcgg gatccagccc gaggtggcgg
     7201 gcgagattga ccaccgagaa caacacatcg cccaactcct cccgagcccg ggtcggatca
     7261 cccaagacct cctccaactc tcccaattcc tcccggacct ttggcagcac ctggagcgcc
     7321 tcgtcccaat cgaagccgac cttggccgca cgattttgga tcttcgaggc ccgttgcagg
     7381 gcgggcacac ccgaaggaac tccgtcgagc gccgactctc gtgccgagcc gcgctcctcc
     7441 tccttgatcc ggtcccagtt ggacttcacc tcttcggcag aaccgacctc gacatcgccg
     7501 aaaacatggg gatgacggcg gaccagcttc tgacgcatca cctcggcaac gtcgtcgatg
     7561 ttgaaggcgc ccgc
//
LOCUS       DBNHXU010000113         1501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5394629, whole genome shotgun sequence.
ACCESSION   DBNHXU010000113 DBNHXU010000000
VERSION     DBNHXU010000113.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5394629"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>1501)
                     /locus_tag="AC161X_02970"
     CDS             complement(<1..>1501)
                     /locus_tag="AC161X_02970"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR4988542.1"
                     /translation="LVVGIVWLGVLFASAIRNRGGEEVAPNLKPGITDQEMETRRLEK
                     GQKAAIAFSAILAISLPLYFLGETDRQENFVNEFEVAAQDRGEHIVEEFKCFDCHGPE
                     GVGGSARYVEKRSGVTVAWAVPSLNDVLFRYDENELNFWITYGRGNTPMPAWGLAGGG
                     PLNEAQVSDVVAYLKTIQVSQNEALAKVEPGVEAQLERLDTADETVATAIITQAQVVA
                     DIDAAPDRLVIVTPLADRAKEVLKTAGEGQDTDDDGISDSAEVELSEISAQAVEELTV
                     VEPVALDPETPDAELAEQAMTSLEESVEVAPILQIYLDAVQEAIDGGTVEPSVGISPA
                     AITELEEIAATADKAGVTVPLSINDISDAEALVTALEDAAAADPPVEGAADLAAKADA
                     SITAGSDPDGDGLSSAAEEDITNQMTEANVRTIPGGLTVITLDPTNPASEGGEADDET
                     AARMVGNLESLHTSLKVTSQNFDKLRDQEVGGLEYLVEAQKTAYWEIDFE"
BASE COUNT          256 a          476 c          459 g          310 t
ORIGIN      
        1 cctcgaaatc gatctcccag taggccgtct tctgggcttc cacgaggtat tccaatcccc
       61 cgacctcctg atcccgaagt ttgtcgaagt tctgagacgt caccttgaga gaggtgtgca
      121 gtgattcgag attccccacc attcttgccg cggtctcatc gtcagcctca ccgccctcag
      181 aagccgggtt ggtggggtcg agagtgatca cggtgagacc gcctgggatc gtcctcacgt
      241 tggcctcggt catctgatta gtgatgtctt cctcagccgc cgaggacagg ccgtcaccgt
      301 ctgggtcgga tccggcggtg atggaggcat cagcttttgc ggctagatcc gccgcccctt
      361 caactggcgg atcggcggcg gcagcgtcct cgagggctgt caccagggcc tctgcgtccg
      421 agatgtcatt gatggagaga ggcacggtca caccggcctt gtctgcggta gctgcgatct
      481 cctccaattc agtgatggcc gccgggctga tgccgaccga aggctcgacc gtccccccgt
      541 cgatggcctc ttgcactgca tcgagataga tctgaaggat gggcgcaact tcgacggact
      601 cctccaaaga tgtcatcgct tgctcggcga gctcggcgtc aggcgtctcg gggtcgagcg
      661 ccacaggttc caccacagtg agttcctcga ccgcctgagc cgaaatctcg gagagctcga
      721 cttcggcgct gtcggagatc ccgtcatcgt cggtgtcctg cccctccccg gcggtcttga
      781 gaacctcttt cgcccggtcg gcgagcggag taacgatcac aagacggtcg ggagctgcgt
      841 cgatgtcggc gacgacctga gcctgagtga tgatcgccgt agcaacggtc tcgtcggctg
      901 tatccagcct ttcgagttgc gcctcgacac cgggctcgac cttggcgaga gcttcgttct
      961 gcgacacctg gatcgtcttc aggtaggcga cgacgtctga cacctgggcc tcgttgaggg
     1021 gacctcctcc ggcgagaccc cacgccggca tcggcgtgtt gccccgccca taggtgatcc
     1081 agaagttgag ctcgttctcg tcataccgga aaaggacgtc gttcagggag gggacggccc
     1141 atgccacggt caccccggag cgcttctcca catagcgggc gctccctcca accccttccg
     1201 gcccgtggca gtcgaagcat ttgaactcct cgacaatgtg ctcgcctcga tcctgggcag
     1261 ctacctcgaa ttcgttgacg aagttttcct ggcggtcggt ctcaccgagg aagtagaggg
     1321 gaagggagat ggccagaatg gcggaaaagg cgatagccgc cttctgtcct ttctcgaggc
     1381 gtcgggtctc catctcctgg tcggtgatgc caggtttgag gttgggagct acctcctccc
     1441 caccccggtt ccggatcgcc gaggcaaata gcacgccgag ccagacgatc ccgacgacga
     1501 g
//
LOCUS       DBNHXU010000114         9493 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3807906, whole genome shotgun sequence.
ACCESSION   DBNHXU010000114 DBNHXU010000000
VERSION     DBNHXU010000114.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9493)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9493)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9493
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3807906"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="AC161X_02975"
     CDS             complement(<1..541)
                     /locus_tag="AC161X_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015070352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008661 - 1-deoxy-D-xylulose-5-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase
                     N-terminal domain-containing protein"
                     /protein_id="HIR4988543.1"
                     /translation="MSILETISSPADLRELSLDQLQVLSDEIRALIIDKVSATGGHLS
                     PNLGVVELTLALHRVFDSPNDRIIWDVGHQAYVHKLVTGRREEFDQLRRTGGLSGYPS
                     REESPHDFVENSHASTSLSYALGHALSGYPGYTVAVVGDGALTGGMAYEALNHIAVAR
                     PPRLIIVLNDNGRSYAPTVG"
     gene            complement(565..1860)
                     /locus_tag="AC161X_02980"
     CDS             complement(565..1860)
                     /locus_tag="AC161X_02980"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4988544.1"
                     /translation="MSSGSAPGRTSFAVRLFVSYALAYLILLGVIGWLLFRSGEQGDS
                     TLIILAFGALLGLVVIGLVARRMAAPIAALTQQTHELALSQRGQVAQAEHAIGTLQMV
                     LGALPQGTVLFSADDEVEYSNASAQELLGVVPSSLSGLTPFPFQNAVRECRERRQSVE
                     RVVEHGKPVRRLRAVATPFAGDQRILLVLVDVTDLERVASIRRDFVANASHELKTPVS
                     AIIASSEALQIAVGRNDDSSVGFAQQIDRSARQLDRLVADLLDLSRLERDQPEMEPMS
                     LDLLVREEVERLRPAAEEKGIDLTFDLSPTQIAGSRRDIAIAVRNLLDNAVRYTGAGG
                     SAGAALETVDDEVVLRISDNGEGIPTKDLGRVFERFYRVDAARSRSTGGTGLGLAIVK
                     HVVEGHGGAVAVESELGRGSVFTVRLPALNGDNGGSELA"
     gene            complement(1853..2566)
                     /locus_tag="AC161X_02985"
     CDS             complement(1853..2566)
                     /locus_tag="AC161X_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018223990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR4988545.1"
                     /translation="MTAPPDIRRPRILLVEDEPGLVDSVRYALDSEGFDVFVAESGVG
                     GLESARVNQPDLVLLDLMLPGMSGLDVCRQIRSSSDVPIIMLTAKDAESDKVAGLELG
                     ADDYMTKPFSMRELIARVRAQLRRATKSGALSESNEVLRGGAIELDIDAHQARLNGDA
                     VDLRPKEFELLESLMRRKNRLAARHTLIDEVWGPAYFGDTKTLDVHIKRLRQKLEPDP
                     AGPTHILTVRGLGYKFVDE"
     gene            complement(2550..3254)
                     /gene="phoU"
                     /locus_tag="AC161X_02990"
     CDS             complement(2550..3254)
                     /gene="phoU"
                     /locus_tag="AC161X_02990"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA];
                     GO_process: GO:0030643 - intracellular phosphate ion
                     homeostasis [Evidence IEA];
                     GO_process: GO:0045936 - negative regulation of phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HIR4988546.1"
                     /translation="MTEATDTNLHFHQGLDALEQSVLGMVDRAEQMVAMAVDAVCTSD
                     VALAAQVILLDDDIDRTYLDVHSQWTNLMARFQPLGPDLRKMTVLLQLNQTFERMGDQ
                     GVNIAKVTRLTEGLPRVERMVEQIREMGDLVRPMIRTAIEAYIRKDLGEARLLPAMDQ
                     PVDRLNANMYKEILVVGNNPPMLEWASKILMVARALERIGDQAVDFAEQTAYLITGER
                     AEFVKVAVRSVDDSPT"
     gene            complement(3251..4159)
                     /gene="pstB"
                     /locus_tag="AC161X_02995"
     CDS             complement(3251..4159)
                     /gene="pstB"
                     /locus_tag="AC161X_02995"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019870431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HIR4988547.1"
                     /translation="MTNQEGDGLTTQTDTTSLESAETALAEHAALAKAADADSIPREQ
                     RPLIFDVEGLTVYYGSFRAVRDVDLDIRQNQITALIGPSGCGKSTVLRCFNRMNDLIE
                     TARVEGKVEYHGVDLYGERVDPVEVRRRIGMVFQKPNPFPKSIYENIIYGPKIAGWKG
                     NYDDLVEEALTKAALWEEVKDKLKESGMALSGGQQQRLCIARAIATSPDVVLMDEPCS
                     SLDPIATLKIEELMLDLKKDYTIVIVTHNMQQAARVSDQTAFFSVNVDEVGQRTGYLV
                     EFSQTEQIFTSPTQKQTEDYITGRFG"
     gene            complement(4156..5121)
                     /gene="pstA"
                     /locus_tag="AC161X_03000"
     CDS             complement(4156..5121)
                     /gene="pstA"
                     /locus_tag="AC161X_03000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020466830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HIR4988548.1"
                     /translation="MTADARALKTTEMVRASIEGRRFSPVNALVAFTLLFSLLTSVVI
                     LITLLVDTISTAMPVFQARGLSFVTSSLSPAPETSGIWQGIMGSLALTGFVIAIAFPV
                     GVAAAVYLEEYASDNRVTRIIGANIRNLAGVPSIVYGILGLFLFVNIVMGGFFGMEGG
                     IGGRNVIAGGLTLAVLVLPIMIITASEALRAVPSGLREASLAVGATRWETIRHHVLPV
                     AFPAILTGSVLTIARAFGESAALLLAGAQLSLYFSVASDASFVDLVTNQPYTALPITI
                     FNFARQPQEAFVALTAAAIVVLLVMLLTVNAVAVIMRDRYERRYR"
     gene            complement(5123..6046)
                     /gene="pstC"
                     /locus_tag="AC161X_03005"
     CDS             complement(5123..6046)
                     /gene="pstC"
                     /locus_tag="AC161X_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017882761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HIR4988549.1"
                     /translation="MGQTSGGAVIDLSGDVRRRRRERLVKAIFFAAATFSILISLAIV
                     LALAGKAWSFLTSIELNQLFGDGWFPRRGRFQVSALLAHTLMVTGIAMFVAVPFGLGS
                     AVYLSEYARSGVRRVLKPTLEILAGIPSVVLGFFALQFISPNVVRGLFPDAESANFLA
                     AGIAVGILTIPLVASVSEDAMRAVPLSLREASYGLGAKQWHTALRVVFPAAISGIIAA
                     IILGISRAVGETMVVALAAGLNGIMTFNPTSGGLTMTGAMANLAVGSDQVAGDAAAFQ
                     SLFFVGLVLFLLTLGLNVISGRMVRRFRQRY"
     gene            complement(6115..7143)
                     /locus_tag="AC161X_03010"
     CDS             complement(6115..7143)
                     /locus_tag="AC161X_03010"
                     /inference="COORDINATES: protein motif:HMM:TIGR02136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HIR4988550.1"
                     /translation="MKIRIKSLIGLVAVLALVLAACGGGASEETTTSSSGGDGGSSGS
                     IVISGSSTVEPISARVGEAFDAANPGVATSVEGPGTGDGFARFCNNETDISDASRPIK
                     DEEAADCEAAGVEYVELHVATDGLTVMTNPANDAVSCLAFSDLYAILGPESEGFANWS
                     DANDLAAELSAPNAPYPDESLVIFGPGEESGTYDSFVEFVIAGLSEERLGEGNNFTRA
                     DYNASANDNVIVQGITENPSSLGWVGFAFFEENQDVLKAIEVDSGESGCVAPTTETIA
                     SFEYPLSRPLFIYVKTNDLESRPELSAFVDFFLSDAGLAAVNDAGYVQLPADDMEATR
                     AAWAGVAG"
     gene            complement(7332..9404)
                     /gene="ppk1"
                     /locus_tag="AC161X_03015"
     CDS             complement(7332..9404)
                     /gene="ppk1"
                     /locus_tag="AC161X_03015"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009358 - polyphosphate kinase complex
                     [Evidence IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006799 - polyphosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 1"
                     /protein_id="HIR4988551.1"
                     /translation="MVFISTEVEQFDPEHFLNRELSWLEFSRRVLSFAQRSDIPLLER
                     AKFLAIWANNLDEFFQVRVAGLKEQVSHGVGVTPPDGMTPSQQLAAIRSVVEVQYETI
                     SKEFLSELAPALGTEGIVFSDYPTLDDEDRAFLDAQFRELVFPVVTPLAVDPSHPFPY
                     ISNLSLNMAVFVRNPDTGLARFARVKVPPILPRFVVLPDRERYVPLEQVIAAHLGALF
                     PGMEVLAHHVFRVTRNADIDVEEDEGGDLLMAIESELTQRRWGRVVRLEIHPDMPDEV
                     LDLLMREMGATEDDVYRVDGPLDLSGLWGLYDLDRPDLKFETFPGVTPLRLVASSVDS
                     LDVFEVIRQGDLIVHHPYDSFTPTVEAFIDRAARDPRVLAIKMTLYRNSSSSPVMGSL
                     IKAAESGKQVVALVELKARFDEERNIEWAQRLEDAGVHVMYGVVGLKTHTKLTLVVRR
                     EGEGVARYAHIGTGNYNEKTARTYEDVALLTADPEIGSDLSDLFNYLTGYSRQTSYRK
                     LAVSPNGIRSRITELIEEEASRPDGHITLKMNSLVDARIIESLYEASNAGTRIDLIIR
                     GISCLRPGVPGLSENITVRSIVGRYLEHSRVYRFGARGRPRHYYMGSADLMPRNLDRR
                     VEALAPINDPDLQFRIDEVFDVLMADDLLAWELKSDGTWSRVPVVSGVDAHLVLQELA
                     RVRASAHL"
BASE COUNT         1913 a         2896 c         2966 g         1718 t
ORIGIN      
        1 cacccactgt cggggcgtag gaacgcccat tgtcgttgag gacgatgatg agtcggggcg
       61 gcctggccac cgcgatgtgg ttcagggcct cgtatgccat cccgccggtc aatgcgccgt
      121 ctccgacgac ggcaaccgtg tagcccggat agcccgatag ggcatggccc agggcgtagg
      181 agagcgacgt cgaagcgtgg ctgttctcga cgaagtcgtg gggtgactcc tcacgggacg
      241 ggtatccaga cagcccgccg gtgcgccgga gttgatcgaa ttcctctcgc ctcccggtca
      301 ccagcttgtg gacgtaggcc tgatgcccca catcccagat gatccggtcg ttgggagagt
      361 cgaacacccg atgcaacgcc aaagtgagct cgacgacgcc gaggttcggt gagagatggc
      421 cgccggtggc cgagaccttg tcgatgatga gggcgcggat ctcgtcgctg agcacctgga
      481 gttggtccag gctcaactca cggaggtcgg ccgggctgga aatggtttcg agaatcgaca
      541 tgaggaaccg gcgattagcc ggtgtcaggc tagctctgac ccgccgttgt ccccattgag
      601 agcggggaga cgcacggtga acaccgaccc tcttcccaat tcactctcca cggcaacggc
      661 gccgccatga ccctcgacga catgcttcac gatggcgaga ccgagcccgg ttcccccggt
      721 cgatcgtgac cgagctgcgt cgacccgata gaaccgttcg aacactcgac ccagatcctt
      781 ggtcggtatg ccctcgccgt tgtcggagat ccggagcacc acctcatcat cgacggtctc
      841 cagcgccgcg cccgccgatc ccccggctcc ggtgtagcga accgcattgt cgaggaggtt
      901 gcgcacggca atcgcgatgt ctcggcggct gccggcgatc tgggtcggcg acaagtcaaa
      961 agtgagatcg atgcccttct cctcggcggc tgggcgaagg cgctccactt cctcgcgaac
     1021 caagaggtcg aggctcattg gctccatctc cggttgatcc ctttccagtc gggacaaatc
     1081 gaggaggtca gccaccaatc ggtcgagttg ccgggccgag cggtcgatct gttgggcgaa
     1141 accgaccgaa gaatcgtcgt tccgtccaac cgcgatctgg agggcttcgg aagaggcgat
     1201 gatcgccgac accggtgtct tcaactcgtg tgacgcattc gccacgaagt cgcgtcgtat
     1261 cgaagccacc cgttcgaggt cggtcacgtc gaccagtacc agcagaatcc gctggtcccc
     1321 tgcgaaggga gtggcaacgg cacgcaggcg acgcaccggc tttccatgct cgacgacccg
     1381 ctccacagac tgccgcctct ccctgcactc ccgcaccgca ttttggaaag gaaagggcgt
     1441 cagaccggaa agactgctcg gaaccacgcc caggagctcc tgtgccgaag cattggagta
     1501 ctcgacctca tcgtctgccg agaacaggac cgtcccttgg ggcagggctc cgagcaccat
     1561 ttgcagggtt ccgatggcat gctccgcctg cgccacctga cctcgctggc tcagggccag
     1621 ctcgtgagtc tgttgggtca gggcggcgat gggtgcggcc atgcgacggg ccaccagccc
     1681 aatcacgacc agtcccagga gagcgccgaa ggcgaggatg atcagggtcg agtcgccctg
     1741 ctcccccgat cggaagagaa gccatccgat cacaccgagc agaatcagat aagccagggc
     1801 gtaggaaacg aacaaacgaa ctgcgaagga tgtcctgccc ggagctgagc cgctactcat
     1861 ccacgaattt gtagccgagc ccgcgcaccg tgaggatgtg ggttggccca gctggatcgg
     1921 gttctagctt ctggcgcagt ctcttgatat gaacgtccag ggtcttggtg tccccgaaat
     1981 aggcagggcc ccacacctcg tcgatcaggg tatgacgggc ggccaggcgg ttcttgcgcc
     2041 gcatcaacga ctcgaggagc tcgaactcct tgggtcgcag gtcaaccgca tcaccattca
     2101 atcgtgcctg gtgcgcgtcg atgtcgagct cgatcgcacc gccccggaga acctcgttcg
     2161 actctgatag cgccccactc ttggtggccc gccgaagctg ggcgcgaacc cgagcgatga
     2221 gctcccgcat cgagaagggc ttggtcatgt agtcatcggc ccctagttcc agaccggcaa
     2281 ccttgtccga ctcggcgtcc ttggcggtga gcatgattat cgggacgtcg gacgacgagc
     2341 ggatctgccg gcagacatcg agccccgaca tcccgggaag catcaggtcg agcaggacca
     2401 ggtcgggttg gttgactctc gccgactcga gcccgccgac tccggactcg gccacgaaca
     2461 catcgaaacc ctcactgtcg agcgcgtacc ggactgagtc gaccaacccc ggctcgtcct
     2521 caacaaggag tattcggggc cgccgaatgt caggtggggc tgtcatcaac agacctgacc
     2581 gcgaccttga cgaactcagc ccgctcaccg gtgatcaagt aagccgtctg ctctgcgaag
     2641 tcaaccgcct ggtcgccgat gcgctccagc gcccgggcca ccatcaggat cttgctcgcc
     2701 cactcgagca ttggagggtt gtttcccacg acgaggatct ccttgtacat attggcgttg
     2761 aggcggtcca caggctgatc catggcgggc aggagcctgg cctcgccgag gtccttgcgg
     2821 atatatgcct cgatcgcggt gcggatcatc ggccgcacca ggtcgcccat ctcacgaatc
     2881 tgctcgacca ttcgttccac tctgggtaag ccttcggtga gcctggtcac cttggcgatg
     2941 ttgacgcctt ggtcgcccat ccgctcgaag gtttggttga gctggaggag aaccgtcatc
     3001 ttgcgcaggt cagggccgag tggctggaac cgggccatga gattggtcca ctgactgtgg
     3061 acgtcgagat aggtccgatc gatgtcatcg tcgagcaaga tcacctgggc ggcgagagcg
     3121 acgtcgctgg tacagacagc atccacggcc atggccacca tttgctccgc gcggtcaacc
     3181 atgcccagca cactctgctc taacgcatca agaccctgat gaaagtgaag attggtgtct
     3241 gtggcttcag tcatccgaat ctccccgtta tgtagtcctc ggtctgtttc tgggtcggag
     3301 aggtgaagat ctgctcagtc tggctgaatt cgacgaggta accagtccgc tggccgactt
     3361 cgtcgacgtt gaccgagaag aaggcggtct gatccgacac ccgagcagcc tgttgcatgt
     3421 tgtgggtgac gatcacgatc gtgtagtcct tcttcaggtc gagcatgagc tcctcgatct
     3481 tgagggtggc aatcggatcc agcgacgagc agggttcgtc catgagtacc acatccgggc
     3541 tggtggcgat ggcccgagca atgcacagcc gctgctgctg accaccggac agggccattc
     3601 ccgactcttt gagcttgtcc ttgacttcct cccatagagc agccttggtc agagcctcct
     3661 cgaccaaatc gtcatagttg cccttccaac cagcgatttt ggggccatag attatgttct
     3721 cgtagatgct cttcgggaag gggtttggct tctggaacac catcccgatg cgtcgccgaa
     3781 cttcgaccgg atcgaccctc tccccgtaga gatcgacgcc gtgatactcg accttcccct
     3841 ccacccgggc tgtctcgatc aggtcattca tccggttgaa acagcgcaag acagtcgact
     3901 tgccgcaacc cgaaggcccg atcagggctg tgatctggtt ctggcggatg tctaaatcga
     3961 catcacgcac cgcccggaag gagccgtagt aaacggtcaa cccttcgaca tcgaagatca
     4021 acggccgttg ttcacgtggg atggagtcgg catctgccgc tttggccaac gcggcatgtt
     4081 cggctagagc ggtctcggcc gactcgaggc tggtggtatc ggtttgcgtg gtcaacccgt
     4141 cgccttcctg gttggtcatc ggtatctccg ctcgtagcga tcacgcatga tgactgccac
     4201 tgcattcacc gtgagcagca tcaccaaaag gacgacgatg gcggccgcgg tcaaggcgac
     4261 gaacgcctcc tgcggctggc gagcgaagtt gaaaatcgtg atcggcaacg cggtataagg
     4321 ctggttggtc accaggtcga cgaacgaagc atcgctggcg acactgaagt aaagcgacag
     4381 ttgggcaccg gctagcagca gggctgccga ctccccgaag gcacgggcaa tggtcaagac
     4441 cgacccggtg aggatggctg gaaacgccac cggaagcacg tgatgtcgga tggtctccca
     4501 acgagtcgcc ccaaccgcga gggaagcttc acgcaacccc gaggggacgg ctcggagtgc
     4561 ttctgaggcg gtgatgatca tgatcggaag caccagcacc gccagggtca ggcccccggc
     4621 gatgacgttt cgaccgccga tcccgccttc catcccgaag aaaccaccca tcacgatgtt
     4681 cacgaaaaga aagagaccga ggatcccgta aacgatcgag ggcacgccgg ccagattgcg
     4741 aatgttggcg ccaatgatcc tggtgacccg gttgtcggaa gcgtattcct cgaggtagac
     4801 cgccgccgca accccgaccg ggaaggcgat ggcgatcacg aaaccggtta gggccagcga
     4861 acccatgatc ccctgccaga tccccgacgt ctccggggcg ggtgataacg aggacgtcac
     4921 gaacgagaga cccctggctt ggaacacagg catcgcagtc gaaatggtgt cgacgagaag
     4981 agtgatcagg atcaccaccg aggtgagcag ggagaaaagg agcgtgaatg ccaccaaggc
     5041 attgaccggg ctgaatcgtc gaccctcgat ggaagcacga accatctcgg tcgttttcag
     5101 ggctcgggcg tcggcggtca actcaatacc tctgcctgaa gcggcggacc atgcgcccgc
     5161 tgatgacgtt gagcccaagg gtgagcagga atagaaccag cccgacaaag aacaaggact
     5221 gaaaggcggc cgcatcgccg gcgacttgat cagagccgac ggcgagattg gccatggcac
     5281 cggtcatggt cagacctccc gaagtggggt tgaaggtcat gatcccattg agaccggcag
     5341 ccaaggcaac caccatggtc tcaccgacag cccgcgagat gcccagaatg atggcggcga
     5401 tgatcccgga gatcgcagcc gggaacacca cgcgaagggc ggtgtgccac tgcttggccc
     5461 cgagcccata cgacgcctca cgaagtgaaa gggggactgc ccgcatcgcg tcctccgaga
     5521 ccgaggcgac cagtgggatg gtcaggatcc cgactgcgat tcctgcggct aggaagttgg
     5581 cgctttcggc gtcgggaaac aacccgcgaa caacgttggg acttatgaat tggagagcaa
     5641 agaagccgag gaccaccgac ggaattccgg ccagtatctc caaggtcggc tttagaaccc
     5701 gacgcacacc ggatcgggcg tactccgaga gatacaccgc tgatccaagt ccgaagggaa
     5761 ccgcaacaaa catggcaatg ccggtgacca tcaaggtgtg agccagaagc gccgacacct
     5821 ggaatctgcc acggcgtggg aaccagccgt ccccgaacaa ctgattgagc tcgatcgagg
     5881 tgagaaacga ccaggccttg ccagcgagcg ccagcacgat ggcaagcgag atcaggatcg
     5941 agaatgtggc tgccgcgaag aagatcgcct tgaccaagcg ctcccgtcgc ctgcgtcgga
     6001 catcgccgct caggtcgatg acagcgcccc cggatgtctg gcccattgcc atgctcgtga
     6061 cagaggatag agggagggac cggatggtgg tccggcccct ccgatctcga ttcctcagcc
     6121 ggccacaccg gcccaggctg ccctggtggc ttccatgtcg tccgccggca gttgcacata
     6181 gccggcgtca ttgactgccg ccaggccagc gtccgacagg aagaaatcga cgaacgccga
     6241 caattcggga cgggattcca ggtcattcgt cttcacatag atgaagaggg gacgggacaa
     6301 cgggtactcg aagctggcaa tggtctcggt ggtcggagcc acgcaacccg actcgccgct
     6361 gtcgacctcg atcgctttca gcacatcctg attctcttcg aagaatgcga acccgaccca
     6421 tcccaatgac gaggggttct cggtgatccc ctggacaatc acattgtcat tggccgatgc
     6481 gttgtagtcg gctcgtgtga agttgttgcc ttcaccaagc cgctcctcgg agagccccgc
     6541 gatgacgaac tcgacgaagc tgtcgtaggt gcccgactcc tctccagggc cgaagatcac
     6601 aagcgattcg tctgggtagg gggcgttggg tgcgctcaac tccgcagcca gatcgttggc
     6661 gtccgaccag ttggcgaaac cttccgactc ggggccgagg atggcatata gatcgctgaa
     6721 ggccagacag ctgaccgcat cgttcgccgg gttcgtcatg acagtcaggc catcggtggc
     6781 tacatgaagc tcgacgtact cgacgccggc cgcctcacag tcggccgctt cctcgtcctt
     6841 gatcggtcgg gaggcgtcgg agatgtcggt ttcgttgttg cagaatcggg caaagccgtc
     6901 accggtgccg gggccctcga ctgaggtggc tacaccgggg ttggcggcgt cgaaggcctc
     6961 acctacccgc gccgagatcg gctccaccgt ggaggatccg gagatcacga tgctgcccga
     7021 gctaccgccg tctcccccag aactggaagt cgtggtctcc tcgctcgccc caccaccgca
     7081 ggcggcgagt acgagggcga ggacggctac caggccgatc aggctcttga ttcggatctt
     7141 catttccctt ggtgctcctt gattgggacc gtttcgatac ccgaaaagct agaaaacggg
     7201 tcttaacggc cccccacgga ggcatgaacg ggagatgaac gttatgggcc gcccgtttct
     7261 ccccccagga gcgggagcga cgttgggggg agtggcccgc agggccgagg ggggcaactc
     7321 cggctcgcgg tctacagatg cgccgaggcc cggacccgcg ccagctcttg gagtacgagg
     7381 tgagcgtcga caccggagac cacgggaaca cgggaccagg tcccatccga tttgagctcc
     7441 caggcgagaa ggtcatcggc catcagcacg tcgaaaacct cgtcgatccg gaattggaga
     7501 tcggggtcgt tgatcggagc caatgcttcg accctgcggt cgaggttccg gggcatcagg
     7561 tccgccgacc ccatgtagta atggcgagga cgccctctcg ccccaaagcg gtagaccctc
     7621 gagtgctcca agtagcggcc aacgatggag cgaaccgtga tgttctccga cagcccgggc
     7681 actcctgggc gaagacaaga gatcccgcga atgatcagat cgatgcgggt ccccgcgttc
     7741 gaggcctcat acagcgactc gatgattcgg gcgtcgacaa gcgagttcat tttcagcgtg
     7801 atgtgcccat cgggccggga tgcctcttct tcaatcaact cggtgatccg gcttctgatc
     7861 ccgttcggcg atacggcgag cttgcggtag ctggtctgac gcgagtagcc ggtcagatag
     7921 ttgaagaggt ctgagaggtc agaaccgatc tctgggtcag ccgtcagaag agcaacgtcc
     7981 tcataggtgc gggctgtctt ctcgttgtag ttcccggtgc cgatgtgggc gtatcgggca
     8041 actccttcgc cctcgcgcct caccaccagg gtcaacttgg tgtgcgtctt gagacccacg
     8101 actccgtaca tcacgtggac cccggcgtct tccaaccgct gtgcccattc gatgttgcgc
     8161 tcctcatcga agcgggcctt gagctcgacc agagcaacca cctgtttgcc ggattcggcc
     8221 gccttgatca gagagcccat caccgggctg ctgctcgagt ttcgatagag ggtcatcttg
     8281 atggcgagga cccgcggatc ccgggcggcg cgatcgatga aggcttcgac ggtgggggtg
     8341 aacgagtcgt aggggtggtg gacgatgaga tcgccctgcc ggatcacttc gaagacgtcg
     8401 aggctgtcca cactggaggc caccagcctg aggggggtca ccccggggaa ggtctcgaat
     8461 ttgaggtccg ggcgatcgag gtcgtagagg ccccatagcc ctgaaaggtc gaggggaccg
     8521 tccactctgt agacgtcgtc ttcggtggcg cccatctccc gcatgaggag atcgagaacc
     8581 tcatcgggca tgtcggggtg gatctcgaga cggaccaccc gcccccaacg tctctgggtc
     8641 agctcggact cgatggccat caagagatcg cccccttcgt cctcctcgac atcgatgtcg
     8701 gcgttccggg tcacccggaa cacgtgatgg gccaggactt ccataccggg gaacaaggct
     8761 ccgagatggg cagcaatcac ttgttcgagg gggacatacc gctctcggtc ggggagcacg
     8821 acgaatcggg gaaggattgg cggcaccttg accctggcaa agcgggcgag gccggtgtcc
     8881 gggtttcgca cgaacaccgc catgttgagg gagaggttgg agatgtaggg gaacgggtgg
     8941 gacgggtcca ctgcaagcgg tgtgaccacg gggaacacca actcccggaa ctgggcgtcg
     9001 aggaatgctc gatcctcatc gtcgagggtg gggtagtcgc tgaagacaat gccctcggtg
     9061 cccagggccg gcgccagctc agagagaaac tccttggaaa tggtctcgta ctggacttcg
     9121 accacgctgc ggatcgccgc caactgctgg ctcggtgtca tcccgtcggg cggagtgaca
     9181 ccaacgccat gagacacctg ttccttgaga ccggcgacac gcacctggaa gaactcgtcc
     9241 aggttgttgg cccagatcgc caagaatttg gccctctcca ggaggggtat gtcgctgcgc
     9301 tgggcgaaag acaggacacg ccggctgaat tcgagccaag acagttcccg gttcaggaaa
     9361 tgctctggat cgaactgctc cacttcggtg ctgatgaata ccacggcact gttatcggtt
     9421 agaggcggcc taccccgaaa ccgccaagta gaaaaagtcg cagatgcatg gtgaccgact
     9481 cgattcggtc ttg
//
LOCUS       DBNHXU010000115         7405 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1030995, whole genome shotgun sequence.
ACCESSION   DBNHXU010000115 DBNHXU010000000
VERSION     DBNHXU010000115.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7405)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7405)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7405
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1030995"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..224)
                     /locus_tag="AC161X_03020"
     CDS             complement(<1..224)
                     /locus_tag="AC161X_03020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017176972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M16 family metallopeptidase"
                     /protein_id="HIR4988552.1"
                     /translation="MHYDLTTLPNGIRVITEPMPSLRSIALGCWIDTGTRDELPNEHG
                     ASHFLEHLLFKGSETLSARQVSEIFDSIGA"
     gene            299..2389
                     /locus_tag="AC161X_03025"
     CDS             299..2389
                     /locus_tag="AC161X_03025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988553.1"
                     /translation="MRGRDQTFLTDLIDPEERAIEAVVSRYQKVAPAVTRFARTLAKN
                     PNLRVRLGSEAAASDDEVVCDPRIFQAAYLRNAPVTPDEVALASALHEVMHLVTTNFD
                     EVREVPESWPVDLENPPDGPVDLLTALERTNKPVAEVLFFSLEDARQERQGMSVYPGA
                     RSVLADLYMSSLSQAIARSGALSQFVLGCFLLTGGYVERDVLEKRFEANAAMALDDGS
                     GQCEMAAEASDPWEVAQLALELEAIARANGLISEAQSMDTHAKRHIAAEAEAEKAQDG
                     VDAVRLTSPILTDADSYQDTRRSAESRSALSDRRGASDIAGEDSTDQLLRVSQAPDVF
                     LPTGQGGKVVVTPIPSSFARFSEEGLASLRAASRRWDVAQRRVSGELFPLFTANQRRG
                     LRSGYDQGDVSPHAALFIGAGLYQRLYERRAVRTRREYAVSLLVDASASMLSPRRTPG
                     SASSWAMPAALLGAWTMAHLCEELQIDFEVALFNRGFAAAPDDTEHSYRHKRSSAAAG
                     LKQTHGTAADRLTSTVNHYLVKPFERRWRDAEPSLAGLFYTAAEPRAAATLARRDPKT
                     SPPVSLFEKAANVDEFNVIHAAERMARLGAEVRVLMVLADGMTRGSVEALAKSVAAVE
                     SGGTTVIGLGIGDHTVDVAYRRHEVVEQPEGLAQAMIDGTRSALRRSLALWGMDTWWL
                     RPSAYRPKQEKSIA"
     gene            2382..3371
                     /locus_tag="AC161X_03030"
     CDS             2382..3371
                     /locus_tag="AC161X_03030"
                     /inference="COORDINATES: protein motif:HMM:NF019348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4988554.1"
                     /translation="MPDTVTFHDPSGEGPTIELARFEIPKTLEDRALRIRKIPEPDPY
                     YIDLGMLYRFAQIEQARRSGAPGLVPLHVAVRGHMGTGKDHDIEQFAAAMGLPYFRIP
                     LTGEVRDVTLIGSTRLQGDGKGGTESRWEDGDITRALRGPSLLNLSELNAAGAETLFA
                     LHGLLDRYAALDLPHGETVALRDDVRIFGTMNPTDLRDYAGTQTLNKAFADRWVIWEK
                     SFPGSEQVEAMLTRRYPELKSEYVEAIARLAVEVNESFDAQDTGVRVETPLSLRSILD
                     RIPTGLIVYQKGPDPLRRTWEEYVLPHVDQFDRDHYETVWNAVVRKGPTVSPF"
     gene            3396..4478
                     /locus_tag="AC161X_03035"
     CDS             3396..4478
                     /locus_tag="AC161X_03035"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0009691 - cytokinin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LOG family protein"
                     /protein_id="HIR4988555.1"
                     /translation="MPYDLGKPELDEKVRELVEATSSNANEELIAELVTTVLKLHRDG
                     ASRADLRMMNTALKEMRYSNLVFSRHREPKVTIYGSARLSEQDPNYQLAEDFAARIVT
                     KGWGVITGAGPGIMEAGTRGAGTDRSYGVNIRLPFEAAPNRYLDQSRTVNFKYFFTRK
                     LAFVKESHAFAILPGGWGTLDEAFELLTLIQTGKSDLHPIVLLEAPGTGYWAPMIEFI
                     QDHQLEGGLISPSDLALFFHTHDPIGACDHIWHFYANYQSQRYAGGRLILRLVRGPDD
                     AQLEQLNKEFADIVVEGEIERAEASQAEISDGDSLESDRIAFVFDRRQFGRLRQLIDH
                     LNDLVGTPESFELPEPMTEEQAERPW"
     gene            4530..5261
                     /locus_tag="AC161X_03040"
     CDS             4530..5261
                     /locus_tag="AC161X_03040"
                     /inference="COORDINATES: protein motif:HMM:TIGR03083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase family
                     mycothiol-dependent enzyme"
                     /protein_id="HIR4988556.1"
                     /translation="MRDILSDLVAEQQFLDQSLQRIPIKIWDMVTPSKPWTVRDTIAH
                     LADFEELAADTIVGGNMIQRWQNASDLDSLRKEAIKKGRGMRPQDVIEWWRGGRAKVV
                     EPLSHMGAESRIEWIDGDMSARTFATYRLMETWAHGLDIYATLGLEVEDTSRIRHVCW
                     LGWKTLPHAFKEAGEDYEPVRVEVIGPGYAKWVYGPAETNQVIKGSASDWARVAVRRT
                     KPETTRLKYEGDYAKKALQVVKAYL"
     gene            5285..6049
                     /locus_tag="AC161X_03045"
     CDS             5285..6049
                     /locus_tag="AC161X_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA family protein"
                     /protein_id="HIR4988557.1"
                     /translation="MIHLLPLPGSPRFNGSMSEVLETAISDASTMREAGFPALLVENF
                     GDAPFRADQVDPETVAGVTLAVEAVMQATGVPVGVNILRNDAISALGIATVTGALFIR
                     VNVLTGVMNTDQGPIVGRAAELLRKRRELGLGVEIWADVMVKHATPPPGLEPGQAALD
                     TVERGLADAVIVSGPGTGAEPDIEVAREIRSVIPKGSRVVIGSGASTGNLSRLVEVAD
                     TVIVGSGIKIDNDANNRVDEAKVAAFIEAAANAGLL"
     gene            6164..6742
                     /locus_tag="AC161X_03050"
     CDS             6164..6742
                     /locus_tag="AC161X_03050"
                     /inference="COORDINATES: protein motif:HMM:NF024878.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004112 - cyclic-nucleotide
                     phosphodiesterase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HD-GYP domain-containing protein"
                     /protein_id="HIR4988558.1"
                     /translation="MLRDLPNDILIKVMEDVIEDLHGHVHRVSDLAVDLGTGLGLGED
                     DLDRLALAGILHDVGKIHLDPGILGKPGPLDENERELMQRHPELGFAMTRNRLDPKVA
                     EAILYHHERFDGTGYPFGLVGMEIPILSRIVLVSDAFDAMTSTRAYQPALPVSFAINE
                     IRKHTGTQFDPAVVEIFLELAAVERLPLVMSA"
     gene            6804..>7405
                     /locus_tag="AC161X_03055"
     CDS             6804..>7405
                     /locus_tag="AC161X_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR4988559.1"
                     /translation="MDPLAGFNPATRTWFEASFAEPTPAQQLGWPAIAGGAHTLIHAP
                     TGSGKTLAAFLWTLDRLLSEPQPERSARCRVLYISPLKALAHDVERNLRAPLAGIRHA
                     SQRLSPEPLPELETFLRTGDTPAEDRRRMQRQPPDILITTPESLYLILTSAARSILEP
                     VRWVIVDEVHAIAGTKRGAHLARSLERLEEITEVAPQRIGL"
BASE COUNT         1364 a         2482 c         2257 g         1302 t
ORIGIN      
        1 tccgccccga tcgagtcgaa gatctcggag acctgacgcg cggagagtgt ctccgagcct
       61 ttgaacagga ggtgctcgag aaagtgcgat gccccgtgct cgttcggaag ctcgtcacgg
      121 gtcccggtgt cgatccagca gccgagggcg atggatcgca gggaaggcat cggctcggtg
      181 atgacgcgga tgccattggg aagtgtggtg aggtcgtaat gcaccccggg agtgtattgg
      241 gcgataggcc gtaggccgcc atcgggctgg tttgacactc atgagacaat gacccgctat
      301 gcgtggccgc gaccagacgt tccttaccga cctgatcgac cccgaagagc gggcgatcga
      361 agcagttgtc agccgatatc agaaggtcgc gcccgcagtc actaggttcg ccagaacact
      421 cgccaagaac cccaacctcc gagtccggct cgggtcggag gcggctgctt cggacgacga
      481 agtggtgtgc gacccacgga tcttccaggc cgcgtacctt cgcaatgcgc cagtcacacc
      541 tgacgaggtc gcactcgcat cggctcttca cgaggtgatg cacctggtca ccaccaactt
      601 cgacgaggtc cgtgaggttc cggagagctg gccggtcgac ctcgagaacc ctcctgacgg
      661 ccccgtcgac cttctcaccg ctctggaacg gaccaacaag cccgtggccg aggtcttgtt
      721 cttctccctg gaggacgccc gccaggagcg tcaggggatg tcggtgtacc ccggcgcccg
      781 atcggtcctg gccgacctct acatgtcctc gttgtcacag gcgattgccc ggtccggtgc
      841 tttgagccag tttgtgctcg gctgcttcct cctcaccggt ggttacgtgg agcgtgatgt
      901 cttggagaag aggttcgagg cgaatgctgc catggccctc gacgatgggt ccggccaatg
      961 tgagatggcc gccgaagcca gtgatccatg ggaggtcgcc cagctcgccc tcgagctcga
     1021 agccatcgcc cgggccaacg ggttgatctc ggaggcgcag tccatggaca cccacgccaa
     1081 acgccacatc gccgccgaag ccgaagccga gaaggctcaa gacggtgtcg atgcggtgag
     1141 attgacttcg ccgatcctca ccgacgccga ctcctatcag gacacgaggc gctccgcgga
     1201 gtcccgctcc gccctttccg atcgccgcgg tgcctcggac atagctggtg aggattccac
     1261 cgaccagctg cttcgggtaa gccaggcccc cgacgtgttc cttcccaccg gacagggagg
     1321 gaaagtggtg gtcaccccga tcccgtcgag ctttgcccgc ttctcggagg aaggcctggc
     1381 gtcgttgcgc gcggcgtcgc gacgctggga tgtggctcag cgcagggtgt ccggcgagtt
     1441 gttccccctg ttcaccgcca atcaacgccg gggtctccga tcgggttacg accagggcga
     1501 cgtgtcgccg cacgccgcgc tcttcatcgg tgccggcctc taccagcgtc tctacgagcg
     1561 gcgggcggtg cggaccagac gggagtatgc ggtgtccctg ctggttgatg cctccgcctc
     1621 catgctcagc ccgcggcgca cccctggctc agcatcgtcg tgggcgatgc cggccgccct
     1681 tctcggggcc tggaccatgg ctcacctctg cgaagagctc cagatcgact tcgaagtggc
     1741 gttgttcaat cggggcttcg ccgctgcgcc ggacgacacc gagcacagct accgacacaa
     1801 gcgctcgtcc gccgcggccg ggctcaagca aacccacggg actgccgccg accgactgac
     1861 ttcgacggtc aatcactacc tggtcaagcc attcgagcgg aggtggcgcg acgcagagcc
     1921 ctcgctggcc ggcctcttct acacggccgc cgagccgaga gcggccgcca cgctggcccg
     1981 tcgcgacccg aagacctccc cgccggtctc ccttttcgaa aaggcagcaa acgttgacga
     2041 gttcaacgtc atacacgccg ccgagcgaat ggcccgcctc ggagccgagg tgagggtcct
     2101 gatggtcctc gctgacggca tgacccgggg ctcggtcgag gcactcgcta aatccgtcgc
     2161 cgccgtagag agcggtggca ccactgtcat cggccttggc atcggagacc acacggtcga
     2221 tgttgcctat cgccgccacg aggtcgtgga gcagcccgaa gggctggccc aggccatgat
     2281 cgacgggacc cggagcgccc tgcgccgcag tctcgcactt tggggcatgg acacctggtg
     2341 gctgcgcccc tccgcttatc gacccaaaca ggagaaatcc attgcctgac accgtcacct
     2401 tccacgaccc gagcggagag gggccgacca tcgagttggc ccgattcgag atacccaaga
     2461 ccctggagga ccgggcgctc aggatccgca agatccccga gccggatccc tattacatcg
     2521 acctggggat gctctatcgg tttgcccaaa tcgagcaggc gcgacgctcg ggtgcccctg
     2581 gtctggtgcc cttgcacgtc gcggtgcgag gccacatggg caccggcaag gaccacgaca
     2641 tcgagcaatt cgccgccgcc atgggactcc cctacttccg gatcccgttg accggtgagg
     2701 tgcgtgacgt gaccctgatc gggtcgaccc ggctccaggg tgacggcaag ggcgggaccg
     2761 agagccgttg ggaggatggc gacatcacgc gcgcccttcg cgggccgtcg cttctcaacc
     2821 tctccgagtt gaacgcggcc ggcgccgaga ctctgttcgc cctccacggg cttctggatc
     2881 gttatgcggc cctcgatctt ccccacggag agacagtcgc cctccgcgac gatgtgcgga
     2941 tcttcggaac gatgaacccc accgaccttc gtgactacgc cggcacccag actctgaaca
     3001 aggcattcgc tgatcgctgg gtgatttggg agaagagctt tcccggcagc gagcaggtgg
     3061 aagcgatgtt gacccgtcgt taccccgagc tgaagtcaga gtacgtggaa gccatcgcca
     3121 ggctggctgt cgaggtcaac gaatcgttcg acgcgcagga caccggtgtc cgggtggaga
     3181 cgccgctctc cctccgttcg atcctggatc ggatccccac cggtctcatc gtctaccaga
     3241 agggccccga tcccctgcgt cgcacctggg aggaatacgt gctgccccat gtcgaccaat
     3301 tcgaccgtga ccactacgag acggtgtgga atgccgtcgt cagaaagggc cccacagtca
     3361 gcccgttctg accgtattgg atatcgtcgc aacccatgcc ctacgacctc ggcaaaccag
     3421 agctcgacga gaaggtgcgg gagctcgttg aagcaacctc gagcaacgcc aacgaggagc
     3481 tgatcgccga gttggtgacg acggtcctca agttgcatcg ggatggcgcg agccgggccg
     3541 acctgcgaat gatgaacacc gccctgaagg aaatgcgcta ttcgaatctg gtgttctccc
     3601 gtcaccgcga gccgaaggtc accatctacg gctcggctcg cctcagcgag caggacccca
     3661 actaccagct tgccgaagac ttcgcggcga ggatcgtgac caaagggtgg ggggtgatca
     3721 ccggtgccgg gcccgggatc atggaggcgg gcacccgtgg cgccggcacc gaccggtcct
     3781 acggggtgaa catcaggctg ccattcgagg cggcgcccaa ccgctatctc gaccagtcgc
     3841 gcacagtcaa tttcaagtac ttcttcactc gcaagctggc cttcgtcaag gagtcacatg
     3901 ccttcgcgat cctgcccggc ggatggggca ccctcgacga ggcgttcgag cttctgacac
     3961 tgattcaaac cgggaagagc gacctgcacc cgatcgtcct cctggaagca cccggcaccg
     4021 gctattgggc gccgatgatc gaattcatcc aggatcacca attggagggc gggctgatct
     4081 ccccaagtga cctcgccctc tttttccata ctcacgaccc gatcggggcc tgtgaccaca
     4141 tctggcattt ctacgccaac taccaatccc agcgttatgc cggagggcgg ctcatcctgc
     4201 gtctggtgcg cgggcctgac gacgcccaac tcgagcagct gaacaaggag ttcgcggaca
     4261 tcgtcgtcga gggtgaaatc gagcgggccg aggcatcgca ggccgagatc agcgacggcg
     4321 actctttgga atcagatcgg atcgcttttg tctttgatcg gcgtcaattc ggccgtctcc
     4381 gtcagctgat cgaccacctg aacgacctgg ttggaacacc ggaatccttc gaattgcccg
     4441 agcctatgac cgaggagcaa gcggaaagac cctggtgaga gggtagtttc gcctgggaat
     4501 agcttgcggg gggctactac cattggaacg tgcgcgacat cctttccgac ctggtggccg
     4561 aacaacagtt cctcgatcag tccctacaaa ggatcccgat caagatctgg gacatggtga
     4621 ccccctcgaa accgtggacc gttcgcgaca ccatcgccca ccttgctgac ttcgaagagc
     4681 tcgccgccga caccatcgtc gggggcaaca tgatccagcg ttggcaaaat gcctccgacc
     4741 tcgactcctt gcgcaaggaa gcgatcaaga aaggccgcgg gatgcggccc caggatgtca
     4801 tcgaatggtg gcgtggcggg cgggccaaag tcgttgagcc actctcccac atgggggcgg
     4861 agtcgaggat cgaatggatc gatggggaca tgagcgcccg cacctttgcc acctaccgct
     4921 tgatggagac ctgggctcac ggcctggaca tctatgccac cctcggcctc gaagtcgagg
     4981 acacctcccg gatccgccac gtctgctggc tcggctggaa gaccctcccc cacgcattca
     5041 aagaggcagg cgaggactac gagccggtga gggtcgaggt gatcggccct ggatatgcaa
     5101 agtgggtcta cggcccggcc gagaccaacc aggtgatcaa gggctccgcc tcggactggg
     5161 cccgggttgc ggtgcgccgg accaagccag agacaacccg cctcaagtac gaaggcgact
     5221 acgccaagaa agccctccag gtagtcaagg cctatctgtg agtgggcttc cccacctggt
     5281 ggggatgatt catcttctcc cactcccggg gtcgccccgt ttcaatggct cgatgagcga
     5341 ggtgctcgag actgcgatct ccgacgcttc cacaatgcgc gaagcaggtt tcccagcact
     5401 cttggtcgag aactttggcg acgccccatt ccgggcggac caagtcgacc ctgagacggt
     5461 tgccggcgtg acgctcgctg tcgaggccgt gatgcaggcc acaggtgtcc cggttggcgt
     5521 caacatcctg cgcaacgacg cgatcagcgc tctcggtatc gccaccgtga ccggtgccct
     5581 cttcatccgt gtcaacgtgc tcaccggtgt gatgaacacg gatcagggcc cgatagtggg
     5641 gcgtgccgca gagctgcttc gcaagcgccg cgagctcggt ctcggtgtcg agatctgggc
     5701 cgatgtgatg gtgaagcacg ccaccccacc gcctggcctg gagccgggtc aggctgccct
     5761 cgacaccgtc gagcgaggcc tcgccgacgc ggtgatcgtc tccggaccgg ggacaggtgc
     5821 cgagcccgac atcgaggtgg cccgcgagat tcgctcggtc atccccaagg gcagtcgagt
     5881 tgtgatcgga tccggcgcaa gcaccggaaa cctctcccga ctggtggagg tcgccgacac
     5941 tgtgatcgtc ggatccggga tcaagatcga caacgacgcc aacaatcgag tcgatgaggc
     6001 gaaggttgcg gccttcatcg aggcggccgc gaacgccgga ctgctctaga agtcgatagg
     6061 tctagcgcac tacacaaaag gattagtccc gtcgacccat accggtctca tccaaactgg
     6121 gcagacttcg catgtggtac ggacgacgcg ggattggtga gtcatgctca gggatctgcc
     6181 caacgacatc ttgatcaagg tcatggagga cgtaattgaa gacctccacg gccacgtgca
     6241 tcgtgtctca gatctcgcgg tagacctcgg aaccggactc ggcctcggag aggacgacct
     6301 cgaccgcctt gcccttgctg gaatccttca cgatgtcggc aagatccatc tcgatcctgg
     6361 aatcctcggc aagccgggcc ccctcgacga gaacgagcgg gaattgatgc agcgccaccc
     6421 cgagctcggc ttcgcgatga cccgcaaccg cctcgacccc aaggttgccg aagcgatcct
     6481 ctaccaccac gagcgatttg atggcaccgg ctatcccttt ggtctcgtcg gcatggagat
     6541 cccgatcctg tccaggatcg tgctcgtctc cgacgccttc gacgccatga cctcgacccg
     6601 ggcctatcag cccgccctcc cggtatcctt cgccatcaac gagatccgca agcacaccgg
     6661 gacccagttc gacccagccg tggtcgagat cttcctcgag ctggcagccg tggagcgtct
     6721 gccactcgtc atgagcgcct gagtttctgc cgactccccg gccgaccggc gagcttgtcc
     6781 ttctctcctg ataccttccc ggtgtggacc cactcgccgg tttcaatccg gccacccgca
     6841 cctggttcga agcatctttc gccgagccga cgccggccca gcaactcgga tggcctgcga
     6901 tcgccggtgg cgcccacacc ctgatccacg ccccaaccgg atccggcaag acactcgctg
     6961 ccttcctctg gaccctcgac cgcctcctct ccgagcccca accggagcga tctgcccgct
     7021 gccgggtgct ctacatctcg ccgctcaagg ctctggctca tgacgtagag cgcaaccttc
     7081 gcgcccctct cgccgggatc cgccacgcct cgcaaaggct gtctcccgag cccctgcccg
     7141 aactcgagac cttcctccgg acgggcgaca ctccggccga ggaccgacgg cgcatgcaac
     7201 gtcagccgcc tgacatcctg atcaccacgc ctgaatccct ctatctcatc ctcacctcgg
     7261 ccgcccgctc cattctcgag ccggttcgct gggtgatcgt cgatgaggtc cacgccatcg
     7321 ctggcaccaa gaggggcgcc cacctggcac ggtccctgga gcgactcgag gagatcaccg
     7381 aggtcgcgcc acaacgcatc gggct
//
LOCUS       DBNHXU010000116         1807 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2816283, whole genome shotgun sequence.
ACCESSION   DBNHXU010000116 DBNHXU010000000
VERSION     DBNHXU010000116.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1807)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1807
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2816283"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1033)
                     /locus_tag="AC161X_03060"
     CDS             complement(<1..1033)
                     /locus_tag="AC161X_03060"
                     /inference="COORDINATES: protein motif:HMM:NF012869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HIR4988560.1"
                     /translation="MRVGVRQSLKLLQTIIPRGQRSKWLAVAALGLVVAAAESVSAVL
                     IAVLVDAMMGDASTSFDLPIIGDVSEILPGETPADQLRFLAIGIAIFFLLKAGLSLTK
                     TYVQARVAQNTGARLSNRLFEGYLTMPYSFHISHNSSELMRNASWAADEVVSNFLTPI
                     ANLITQGAMLIFLLGVLVAAAPTVTLITIGLLVPLVLLSLRAVASVLHRLGNITKHTV
                     KETLSSLQQSLAGIRDIKVLGRERFFAEEFRDNRRLLARAKYMYPTVSSVPSLVIETL
                     LVVFILGFVAFGSAETGVAGASLPVLGLFAYAGLRMIPAMSAIVSSVNQIRYGGAAVQ
                     TVATNLEMID"
     gene            complement(1092..>1807)
                     /locus_tag="AC161X_03065"
     CDS             complement(1092..>1807)
                     /locus_tag="AC161X_03065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988561.1"
                     /translation="RLSLLASLFALAVGGWMARPPGGSARAPFGWILIAAGAITMVLR
                     GGFADIGWLAALAPIVTILIGAALAAWAKRLPQDVLGIMVAISAFGIWATVPETETAR
                     ALLGASIPLAVATLPPIRARLGYSGAFALAGLLVWVVATGGEARSASIVGGWACIGAI
                     AVLPLYNPPVVSLVGRKRALTVLIHALMVVVASRVIGLWESGGLALLAVIVLFVGGYW
                     LLSLLPSGRARKTAATEAS"
BASE COUNT          388 a          570 c          570 g          279 t
ORIGIN      
        1 tgtcgatcat ctccaggttg gtggcgaccg tctggaccgc tgctccgccg tatcgaatct
       61 ggttgacgga cgacacgatg gcggacatgg ccgggatcat tcgcaacccg gcataggcaa
      121 acagtccgag caccggcagg gaggcaccag ccactccggt ttcagcgctc ccaaaggcga
      181 cgaatccgag gatgaacacc accaggagcg tctcgatgac caggctgggt accgatgaca
      241 ccgtcgggta catgtacttg gctcgcgcca gaagcctccg gttgtcacgg aactcctccg
      301 cgaaaaagcg ctcccttccc aggactttga tgtcgcggat cccggcgagg ctttgctgca
      361 acgacgacag ggtctccttc acggtgtgtt tggtgatgtt gccgagacga tgcaggaccg
      421 atgccacggc tcgcagggag agaaggacca gcggcaccag aagcccgatc gtgatcagag
      481 tcacggtcgg agccgctgcg accaggactc ccaggagaaa tatcagcatc gcaccctgtg
      541 tgatcaggtt ggcgatggga gtcagaaagt tggacaccac ctcgtcggcc gcccatgagg
      601 cgtttctcat caactctgag ctgttgtgag agatatgaaa gctgtagggc atcgtgaggt
      661 agccctcgaa gagccggttg gagagccggg cgccggtgtt ctgagccacc cgggcctgga
      721 catacgtctt ggtcagagaa agccctgcct tcaacaagaa gaagatggca atcccgatgg
      781 caagaaacct cagctggtcg gctggcgtct caccagggag gatctcgctc acgtcgccaa
      841 tgattgggag gtcgaacgat gtcgaagcgt ctcccatcat tgcgtcgacc aggacggcga
      901 tcaggaccgc ggacaccgat tctgcggctg ccaccactaa gcccaatgct gccaccgcca
      961 gccatttgga ccgctggcct cgggggatga ttgtctgcag cagcttcaga ctctgacgga
     1021 cacccactcg catggcatca gggtaggtga cgggattgcc aatacctatg ccggtctgcc
     1081 catgtaatgg tctatgaggc ctcagtggcg gctgtcttgc gggcgcgacc cgacggcagc
     1141 aggctcaaca accagtagcc ccccacaaat agcacgatga cggcaagcaa ggccaggcca
     1201 cccgactccc aaaggccgat cacccgcgag gcgaccacca ccatcagggc gtggatcaac
     1261 acagtgaggg ctcgcttgcg gccaacgagg gagaccaccg gcgggttgta gagggggagt
     1321 acggcgatcg cccctatgca ggcccagcca cccacgatgg aggccgagcg cgcctcaccc
     1381 ccggtggcga cgacccagac caagagcccg gccagggcaa aggctcccga gtagccgagc
     1441 ctggcacgga tgggtggcaa cgtcgccaca gcaagcggta tcgatgcccc gagaagcgct
     1501 ctcgcggtct cggtctccgg caccgtggcc cagatcccga aggcgctgat cgcaaccatg
     1561 atccccagca cgtcctgggg gagtcgcttg gcccaagcgg cgagggcggc accaatcagg
     1621 atcgtgacaa ttggcgccag ggccgcgagc cagccgatgt cggcgaagcc acctcgcagc
     1681 accatggtga tcgccccggc tgcgatcagg atccagccga agggagcccg agcactgcca
     1741 cccggtggtc gggccatcca accaccgacg gctagagcga acagcgatgc cagcaaggac
     1801 agtctcg
//
LOCUS       DBNHXU010000117         2572 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4602002, whole genome shotgun sequence.
ACCESSION   DBNHXU010000117 DBNHXU010000000
VERSION     DBNHXU010000117.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2572)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2572
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4602002"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /locus_tag="AC161X_03070"
     CDS             <1..348
                     /locus_tag="AC161X_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006455842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase C-terminal domain-containing
                     protein"
                     /protein_id="HIR4988562.1"
                     /translation="ENRQQEISEITRNLKNLAKELAVPIIAVSQLNRSVETRENRRPR
                     LSDLRESGAIEQDSDVVMFVYRHEYYHPEDQEKKGIAELIIEKHRSGATGTVEMTFQP
                     EFTRFANLGRDVS"
     gene            429..551
                     /locus_tag="AC161X_03075"
     CDS             429..551
                     /locus_tag="AC161X_03075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988563.1"
                     /translation="MVTILRGKAAEKFLKRVYIWDPQQLMARATGDYKRGNERG"
     gene            complement(733..1311)
                     /gene="dcd"
                     /locus_tag="AC161X_03080"
     CDS             complement(733..1311)
                     /gene="dcd"
                     /locus_tag="AC161X_03080"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012924365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006229 - dUTP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HIR4988564.1"
                     /translation="MIFSDRSILEAIDAGVIQIDPFEPSHVQPSSVDLRVGNGFRVFV
                     NHRYSQIDPRSPQADLTQLVEVEADEPFMLHPGEFVLGSTLERVKLGDDVVARLEGKS
                     SLGRLGLLIHSTAGFIDPGFEGHITLELSNVATLPIAIYPEMKIGQVSFYRMTTAADH
                     PYGSPELGSKYQGQSGPTASRSHQDFDPKDTN"
     gene            complement(1364..1434)
                     /locus_tag="AC161X_03085"
     tRNA            complement(1364..1434)
                     /locus_tag="AC161X_03085"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1400..1402),aa:Gly,seq:ccc)
     gene            1543..>2572
                     /locus_tag="AC161X_03090"
     CDS             1543..>2572
                     /locus_tag="AC161X_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011565836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR4988565.1"
                     /translation="MALTTTGTLRNFINGEFTDPIGGETTAVINPATGQEYTRAPLSR
                     EPDVDAACKAALDAFPTWRDATPSERSLALLRIADALEARAEDFAGIETENTGKPYGL
                     ALGDELPPSVDQIRFFAGAARHLQGLSTGEYLANHTSMIRREPIGVCAQVAPWNYPLM
                     MAVWKIAPALAAGNTIVLKPSDTTPATAVALAELCAEFLPPGVFNVITGDRDTGRALV
                     AHEIPSMVSVTGSVRAGKEVAVAAASDLKRVHLELGGKAPVVVFDDADIEAAAEAIAG
                     AGYYNAGQDCTASTRVLAAPGIRRDLADALAEAARGTPTGDPYDENTAFGPLNNPNQR
                     DRVSGFIER"
BASE COUNT          517 a          758 c          834 g          463 t
ORIGIN      
        1 gagaaccgcc agcaggagat ctcggagatc acgaggaatc tgaagaactt ggccaaggag
       61 ttggcggtgc cgatcatcgc cgtctctcag ctaaaccggt ccgtcgagac tcgggagaac
      121 cgtcgtcccc gtctttccga cctccgggag tccggcgcca tcgagcagga ctctgacgtt
      181 gtgatgttcg tctaccgcca cgagtactac caccccgagg accaggagaa gaaggggatc
      241 gccgagctga tcatcgagaa gcatcgctct ggtgcgaccg ggacggtcga gatgaccttt
      301 cagcccgagt tcacccggtt tgccaatctt gggcgggatg tgagctgaga agggagcccg
      361 gccccgatct cggcttctcc tatcagatct ggaaggccgg cgaggttgcg atcagccggc
      421 atggcgaagt ggtcacgatc ctccggggca aggccgccga gaagtttctg aagcgggtct
      481 acatctggga tcctcaacag ctcatggcgc gggcgaccgg cgactacaag cgaggaaacg
      541 aacggggatg atccctcgcc cgcggcgacc cagagggtcg ccgcattttg gaggggttgc
      601 cacttgccct gtgaagccgg ggaagcttcc tgagggggac tcatgtaggg cgtggcgcct
      661 gaacccagtc tgtgcctccc gaaaccctca ggtcgagcag attgggccca aatccaacta
      721 tacgcaaatg cgctagttcg tgtccttggg gtcgaaatcc tgatgggaac ggctggccgt
      781 tggcccactc tggccctggt acttcgatcc gagctcgggt gatccgtaag gatggtcggc
      841 ggcggtcgtc atccgataga agctgacctg tccgatcttc atctccggat atatggctat
      901 cgggagagtt gcgacgttgg aaagctcgag ggtgatgtgg ccttcgaacc cgggatcgat
      961 gaacccggct gtggagtgga tgagcaagcc gagccggccc aaggatgact tcccctcgag
     1021 cctggcaacg acatcatcgc cgagcttcac ccgctccaga gtggacccga gcacgaattc
     1081 gccggggtgc agcatgaacg gctcgtcggc ctcgacttcg accaactgag tcagatcagc
     1141 ctgtggcgag cgaggatcga tctggctgta gcggtggttt acgaagaccc gaaacccgtt
     1201 tccaacccga aggtccacgg acgagggttg gacgtgcgaa ggctcgaacg ggtcgatctg
     1261 aatcactccg gcgtcgatgg cctcaaggat ggatcggtca gagaaaatca aagtcgtgcc
     1321 tggatgttca tagagcgggg cacagcctcg tgtttttcat cagagcgggt gaagggaatc
     1381 gaaccctcgc cccgagcttg ggaagctcgt gttctaccat tgaactacac ccgcaagacg
     1441 ctcgattgta ggtcgcgacc catgccatga atcgggtcga cctgcccgct tcgatcgggc
     1501 caggtaggtt tcactggttc tcactcgcac acggaggtag ctgtggctct tacgacaacc
     1561 gggactctca ggaacttcat caacggggag ttcaccgatc ccatcggtgg agagaccacc
     1621 gcggtgatca acccggcaac cggtcaggag tacacacgcg ctccgctgtc acgtgaaccc
     1681 gatgtggacg cggcgtgcaa ggcggccttg gacgcctttc cgacatggag ggacgccacg
     1741 ccatcggagc ggagcctcgc tctactgagg atcgcggatg cactcgaggc gagggcggag
     1801 gatttcgcag gcatcgagac cgagaacaca ggaaagccct acgggttggc cctcggcgac
     1861 gaactgccgc cttccgtcga ccagatccgg ttctttgccg gggcggcacg ccatctccag
     1921 ggcctctcca ccggcgagta cctggcaaat cacacttcga tgatcaggcg agagccgatt
     1981 ggggtatgtg cccaggtggc gccgtggaac tatccgctga tgatggctgt gtggaagatc
     2041 gcacccgctc tggcggcggg gaacaccatc gttctcaagc cgtcggacac cacacctgcc
     2101 acggcagtgg cgcttgccga gttgtgcgcc gagttccttc caccgggtgt gttcaacgtg
     2161 atcacaggcg atcgggacac cggaagggca ttggtggcgc acgagatccc gagcatggtt
     2221 tcggtgacgg gaagcgtccg ggccgggaag gaagtggcag ttgcggccgc ttcggatttg
     2281 aaacgagtcc atctcgaact tgggggcaaa gcgccggtgg tggtcttcga cgacgccgac
     2341 atcgaagcag cggccgaggc gatcgcgggg gccggctatt acaacgccgg tcaagactgc
     2401 accgcctcaa cgagagtcct cgccgccccg ggaattcggc gtgacctcgc tgatgccctg
     2461 gccgaagcgg ctcggggcac gccgaccgga gatccctatg acgagaacac cgcatttgga
     2521 ccgctcaaca acccgaacca gcgggaccgg gtctcaggtt tcatcgagcg tg
//
LOCUS       DBNHXU010000118         4636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4205392, whole genome shotgun sequence.
ACCESSION   DBNHXU010000118 DBNHXU010000000
VERSION     DBNHXU010000118.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4636
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4205392"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="AC161X_03095"
     CDS             <1..581
                     /locus_tag="AC161X_03095"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018805281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S16 family serine protease"
                     /protein_id="HIR4988566.1"
                     /translation="PKVWKDEASDRTDIPGVATGLAVTGAGGDVLFIEATAMDGEPGL
                     TLTGQLGDMMKESAQIALSYVQAHAEDLGIDAALLKRRFHVHVPAGAIPKDGPSAGVT
                     MLTALVSLLTGRKVKDTVGMTGEITLQGKVLPIGGVKQKVLAAHRAGLKEVIVPRRNE
                     IDLDDVPDLVSEALTFHIADTVDDVLAAALTT"
     gene            673..1065
                     /locus_tag="AC161X_03100"
     CDS             673..1065
                     /locus_tag="AC161X_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988567.1"
                     /translation="MAEIAMGGIHVAVSTKRIAAKECPHAETLTTTTAGVERIVCEDC
                     GHLSFNFPEALSGPVHRRWFARPADHIEEPTPTREPEADWLERFTPVTHATHVDEEAA
                     AIRAFAADERFHIRSRYELHPGPSAAVA"
     gene            1155..1715
                     /locus_tag="AC161X_03105"
     CDS             1155..1715
                     /locus_tag="AC161X_03105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HIR4988568.1"
                     /translation="MSTLENRPNTALLVIDVQNGVVAAAHMRDEVVANVGSLVEKARK
                     EEVPVVWIQHSDEGLERGSDDWLIVPELDPSDTDPLVEKHYGDSFEDTALESVLSGLG
                     IGRLVVVGAQTDECVRSTLHGALVRGYDATLVSDAHTTEDLSEWGAPPPDQVIAHTNM
                     YWSNQKAPGRTAGTVESKDVEFGASS"
     gene            complement(1743..2156)
                     /locus_tag="AC161X_03110"
     CDS             complement(1743..2156)
                     /locus_tag="AC161X_03110"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR4988569.1"
                     /translation="MIFDYFADLNWLAILVAAVAWFAFSAVWYSIPPLSKAWQEAAKV
                     TPGEGPPLAMILIPTFIGYFVTSIVIALIATAIAGDEVINGVALGVALGVGFGVVGAV
                     VNQMYEQKGKSYWLINGANSVIAYTLVAVIVTIWD"
     gene            complement(2195..2995)
                     /locus_tag="AC161X_03115"
     CDS             complement(2195..2995)
                     /locus_tag="AC161X_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/PEP mutase family protein"
                     /protein_id="HIR4988570.1"
                     /translation="MKDGLRQQFADLHREGTFLMPNAWDIGSAKLLASLGFPAIATTS
                     AGLGAALGRMDQQVSLDELVVHVRDLVAAVNVPVNVDAENGYAGTPQGVAEAVDRLAG
                     EGAAGLSIEDYDPSSGIYPLDVAVDRVAAAVEAAKGHGVVLTARAENYLYDIDDIDDT
                     IARLRAFRDVGADVLYAPRIEDPQKIARVVAEVDGPVNVLLMRTTPIVSELADIGVRR
                     ISTGGALALTAYGALAAAARELLSSGTSTYAAAALSNEDRIAAFGGDS"
     gene            3173..4189
                     /locus_tag="AC161X_03120"
     CDS             3173..4189
                     /locus_tag="AC161X_03120"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: protein motif:HMM:NF006089.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase subunit sigma-70"
                     /protein_id="HIR4988571.1"
                     /translation="MTSQTAEIGPTLARAQAGDTIAFASLVDGHRTGLLRHCYRMLGS
                     GPDAEEALQDTLLRAWQRLDTFEARGSFQGWMYRIATNLCIDRLRGRRPRSHPVSHGP
                     SASPGSFPEGADFAIEWVEPIGDASIGLEQDPAHSAEQRERISLAFIAALQRLSARQR
                     AALLLHDVLDFSQNEVAEVLDSSPSAVNSLLFRARETMSASPTPVPADPKDPGVRELL
                     DRYMRAWERADISEFLATVSDDVRISMPPIPTWYQGSETVAKFIETAIFEQVRPYGIT
                     MRPGSANGQPAFATYEPGSDGAMTVSGIQVIEVEDGSKTITGITSFRDPEIAVRCGFS
                     RTIS"
     gene            complement(4203..>4636)
                     /locus_tag="AC161X_03125"
     CDS             complement(4203..>4636)
                     /locus_tag="AC161X_03125"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR4988572.1"
                     /translation="IVGIESASAELARSLGVQHVYLGTDDLPGPFDAIVDATSDSGTP
                     AMSLRYVEPGGRVVLIGISGEPSMIDSRQITIGDMNVVGILGGSAGLGEAITAYANRR
                     VVPDPLVSEVIGLEDVASRLKGERGPNAGPGPKVQVDPTLV"
BASE COUNT          938 a         1450 c         1422 g          826 t
ORIGIN      
        1 agccaaaggt gtggaaggac gaggcgtcgg atcgaaccga catccccggc gtggccactg
       61 gtctggcagt caccggagct gggggcgacg tgctgttcat cgaagctacg gcaatggatg
      121 gggaaccggg tctgacgttg actggtcagc tcggcgacat gatgaaggag tcggcccaga
      181 tcgccttgtc ctacgtccag gcccacgcag aggacctggg catagacgct gctctcctca
      241 agcggcgctt ccacgtccac gtcccggcgg gcgccatccc caaggacggc ccgtcggccg
      301 gagtgaccat gctcaccgcg ctggtatcac tgctcacagg tcggaaggtg aaggacacgg
      361 ttgggatgac cggggagatc acccttcagg gcaaggtgct ccctatcggc ggggtgaaac
      421 agaaggtcct ggccgcccat cgcgccgggc tcaaggaggt catcgtccct cgacgcaacg
      481 agatcgatct cgatgacgtg ccagacctgg ttagcgaagc gctgaccttc cacatcgcag
      541 acacggtcga cgatgtcctg gcggcggcgc tgactaccta ggcatctgtc gagaaccgct
      601 gaccgattta tccacagctt tgggtctgag gacccatagt ggattgccac cgttttcgat
      661 aaacctcgtg ctatggcgga gattgctatg ggaggcatcc acgtcgcggt cagcaccaag
      721 cggatcgcgg ccaaggaatg cccgcacgcg gagacattga ccacgactac tgctggagtg
      781 gagcgaatcg tctgcgagga ctgcggacac ctcagcttta acttccccga ggctctctca
      841 gggccggtgc acagacgatg gtttgcgcgc cccgccgacc acatcgagga gccaacgcct
      901 actcgcgagc ctgaagccga ttggctggag cggttcacac cagtgaccca cgccacgcac
      961 gtcgacgagg aggccgccgc gattcgggcg ttcgcagccg atgagcgctt ccacattcga
     1021 agcagatacg agcttcatcc cgggccttcg gctgccgtcg cctaggacgt tcgccacaac
     1081 gacctcgatg accctggcga tggacgggtt taccctatgt gtccacccga cccagcacga
     1141 gcaaagggga gaccatgagc acactcgaga acaggccgaa caccgcactt ctcgtcattg
     1201 acgtgcaaaa cggcgtcgtc gccgcggctc acatgcgtga tgaagtcgtg gccaatgtcg
     1261 gcagcctcgt ggagaaggca cgtaaggaag aggtcccggt ggtctggatc cagcactccg
     1321 acgagggact tgagcgggga agtgacgatt ggctgattgt ccccgagctc gacccgagcg
     1381 acacagaccc gctcgtcgaa aagcactatg gcgactcgtt cgaagacacc gccctcgaaa
     1441 gtgtcttgtc cggtctcggg atcggccgac tcgttgtcgt cggtgcgcaa accgacgagt
     1501 gcgttcgctc gacgctgcac ggggcgttgg tcaggggtta cgacgcaact cttgtcagtg
     1561 acgctcacac cactgaggat ctttccgaat ggggggcgcc gccgcctgat caagtcatcg
     1621 cacacaccaa catgtactgg tccaaccaga aggcgccagg tcgaaccgcg gggacggttg
     1681 agagcaaaga cgtcgaattc ggcgcgtctt cctgactgct cgacctgccc tcctgcgtgg
     1741 ggtcagtccc agatcgtgac gatcaccgca acgagggtgt acgcgatgac tgagtttgcc
     1801 ccgttgatca gccagtagga cttgcccttc tgctcgtaca tctggttcac gacggctccc
     1861 acaacgccga atccgacacc cagggcaaca ccgagagcga ccccattgat gacctcatca
     1921 cctgcgatcg cggtggcgat caaggcgatc acgatcgagg tcacgaaata cccgatgaag
     1981 gtcggaatca gaatcatggc caggggaggc ccttcgcccg gggtgacctt ggccgcttct
     2041 tgccaggcct tactcagcgg agggatcgag taccagaccg cactgaacgc gaaccatgca
     2101 accgcagcca ccaggatggc gagccaattc aggtctgcga agtagtcaaa gatcatcggt
     2161 catcccttcg atcgagaacg caacgtcagc aaggctacga gtctcccccg aacgctgcga
     2221 tgcggtcttc gttcgacaat gccgcagcgg catacgttga agtgccagac gagagcaatt
     2281 cgcgagcggc cgcggcaaga gccccatagg cggtcaatgc caaagcgcct cctgtggaga
     2341 tccgcctgac acctatatca gccaactcac tcacgatggg cgttgttcgc atcagcagga
     2401 cattgaccgg accatcgacc tcggccacga ccctggcgat cttctggggg tcctcgatgc
     2461 ggggggcgta gagaacgtcg gctcccacgt cacgaaaagc acgcaaccgg gcgatggtgt
     2521 cgtcaatgtc atcgatgtcg tatagatagt tctcggctcg ggcggttagg accactccgt
     2581 gccctttcgc ggcctcgacc gcagcagcca cgcgatcgac ggcgacatcg agtgggtaaa
     2641 tgccagacga tgggtcgtag tcctcgatgg agaggccggc cgcaccctct ccggcgagcc
     2701 gatccacggc ttcggcgaca ccctggggcg tgcccgcgta accgttttca gcatcgacat
     2761 tgacagggac gttgacggcg gcaaccaggt cccggacatg gacgaccagt tcgtcgaggc
     2821 tgacctgctg gtccatgcga cccaacgcgg ctccaagtcc ggcgctcgtg gtggcgatcg
     2881 ccgggaatcc gagcgacgcc aacagcttcg ccgacccgat gtcccaggcg ttgggcatca
     2941 ggaacgtccc ctcacgatgg agatctgcaa actgttgccg taatccatct ttcatacgtg
     3001 tgtctctttc ctcaagatgc gcttggtaag gccatcgaag ataacgagca gcggaaccga
     3061 tgcgaagcca gcagtcaccc ccggcccatc aaacggagat cgctgcagct caccgcaaga
     3121 tttcgagtgc ggatctcgtc atacagtgtg tgacagccgt gatgtgtgac cgatgacgag
     3181 ccaaaccgca gaaatcgggc cgactctcgc ccgggcccag gcgggtgaca ccattgcctt
     3241 cgcgtctttg gttgatgggc atcggaccgg acttctccgg cactgctacc ggatgctggg
     3301 atccggcccc gatgccgaag aggcgcttca agacacactg ctgcgtgctt ggcagcgtct
     3361 cgacactttc gaagcccgag gatcgtttca gggctggatg tatcgaatcg ccaccaactt
     3421 gtgcatcgac cgactgaggg ggcgtcgccc gcgcagccat ccggtgagcc acggtccctc
     3481 agcctcgcca ggttcctttc ccgagggcgc cgacttcgca atcgagtggg tcgagccgat
     3541 cggtgatgcg agcatcgggc tcgagcaaga tccggcacac tccgcggagc aacgagaacg
     3601 gatcagcctc gccttcatcg ccgccctgca acgcctgtca gcgcgtcaaa gggcggcctt
     3661 gctgcttcac gacgtcctcg acttctccca gaacgaagtg gccgaggtac tcgacagttc
     3721 tccatcggca gtcaacagcc tgctcttccg tgcccgagaa acgatgagtg cttcgccgac
     3781 accggtgcct gctgatccga aggatcccgg agtcagggag ctgttggaca gatatatgcg
     3841 ggcctgggag agggccgaca tcagcgagtt cctggcgacc gtcagcgacg atgtccggat
     3901 ttcgatgcct ccgatcccga cctggtacca gggctcggag accgtggcga aattcatcga
     3961 gaccgcaatc ttcgagcagg ttcggccata cggaatcacc atgcgacccg ggagcgccaa
     4021 cgggcaaccc gcattcgcta cctatgagcc gggatcggac ggagcgatga ccgtcagcgg
     4081 aatccaggtg atcgaggtgg aagatgggag caagaccatc acagggatca cctcgttccg
     4141 ggatcccgaa atcgcggtgc ggtgcgggtt ttcgcgcaca atcagctgat actcggagaa
     4201 cctcagacca aggttggatc aacctgcact ttggggcctg gtccggcgtt aggacctctt
     4261 tcccctttca agcggctggc gacgtcctcg aggccgatca cctctgatac cagcgggtcg
     4321 ggcacgacgc ggcggttggc gtaggccgtg atcgcctcgc ccaggccggc agaccctcca
     4381 aggatgccca ccacgttcat gtcgccaatg gtgatctgac ggctgtcgat catgctcggc
     4441 tcgcctgaga tgccgatcag gaccacccgt cctccaggct cgacatagcg gaggctcatt
     4501 gcgggggttc cggaatcgct ggttgcgtcg acgatggcat cgaacggccc gggcaggtcg
     4561 tcagtgccca ggtagacatg ttgaactccc agtgaccggg ccagttcggc gctcgcactt
     4621 tcgatgccga cgatat
//
LOCUS       DBNHXU010000119         2917 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3212998, whole genome shotgun sequence.
ACCESSION   DBNHXU010000119 DBNHXU010000000
VERSION     DBNHXU010000119.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2917)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2917)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2917
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3212998"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1984)
                     /gene="recG"
                     /locus_tag="AC161X_03130"
     CDS             complement(<1..1984)
                     /gene="recG"
                     /locus_tag="AC161X_03130"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: protein motif:HMM:NF008168.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HIR4988573.1"
                     /translation="MATLSRLTEVDVAEVKTLGGSRTPVLAKAGIVTVADLLHHVPRR
                     YIDRSLNESIAEASRGTEVTVVGEVRSVTTRRIPKRKLMIVEAIIFDDSASMKAVWFN
                     QPFRAKQLVEGAQVALSGVVESFNGGLQMKSPQADFLDSSLESLRTGRVVPIHQRIGQ
                     IKPGVLRRAIHNALQRSKPILDTIPAESLERLSLIDRDQAYEHIHFPETMDQVGPARA
                     RLAFDELYRLELTLALRKRRQVLDATGIAHQPTGELVDRFLGGLPFTLTAAQIRVLEE
                     ISGDLRLGHPMHRLLQGEVGSGKTVVAVASLLQGVEGGWQGAIMAPTEVLAGQHYQSV
                     TALLAEAGLSPDPLGPMSDLGMASLFATDGPAVHMALLTGSTARANYRDDIGREQLLA
                     DVANGQVDILVGTHALIQEGVHFSRLGVAVVDEQHRFGVAQRVLLKEKADSVDPDLLI
                     MTATPIPRTLSMTIYGDLDMSIIGEMPPGRTPVKTTVLSPLQESLAWHEVGEAVAEGR
                     QAFVVCPLVEDSPKIEAASAVAEFERLTALLPDLRVGLIHGQLRSTEKDEVMAEFRAG
                     EIDVLVATTVIEVGIDVPNATVMVIEDADRFGLSQLHQLRGRVGRGLHPGVCLLLADP
                     TTEEGERRLAAMASTTDGFVLAEEDLRIRGYGTVFGA"
     gene            2107..2295
                     /gene="rpmB"
                     /locus_tag="AC161X_03135"
     CDS             2107..2295
                     /gene="rpmB"
                     /locus_tag="AC161X_03135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007001659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HIR4988574.1"
                     /translation="MSYRCEICGKEPSYGKTVSFSHKRNNRRWLPNIQRVRVRHGSNT
                     RRARVCTSCIRAGKVEKA"
     gene            2298..2513
                     /locus_tag="AC161X_03140"
     CDS             2298..2513
                     /locus_tag="AC161X_03140"
                     /inference="COORDINATES: protein motif:HMM:NF012534.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HIR4988575.1"
                     /translation="MLQGVVKTYDPVTGAGIIVRDDDRSEVYLRPGALQGSIFRMLRQ
                     GQRLVFDTVEEDGHQYAISVRVGQEGY"
     gene            2686..>2917
                     /locus_tag="AC161X_03145"
     CDS             2686..>2917
                     /locus_tag="AC161X_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019674727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four helix bundle protein"
                     /protein_id="HIR4988576.1"
                     /translation="MTSRFPSAERFGLRSQLRSAAVSISSNIAEGAGRRTNSDTAHFL
                     QIAIGSSCEVESQVLVSNRLGLLPDPGELLDEI"
BASE COUNT          554 a          934 c          895 g          534 t
ORIGIN      
        1 gagcgccgaa cacggtgccg tatccccgga tcctgagatc ctcctccgcg aggacgaagc
       61 cgtccgtggt cgaggccatg gcggccagcc tcctctcccc ctcctcggta gtcgggtcgg
      121 cgaggaggag gcacacaccg gggtgaagac cccgtcccac tctcccgcga agctgatgga
      181 gctggctcag cccgaatcgg tcggcgtcct cgatcaccat cacggtggcg ttgggaacgt
      241 cgattccgac ctcgatgacc gtggtcgcga ccaggacgtc tatctctccg gcccggaact
      301 cagccatcac ctcgtccttc tccgtcgagc gcagctgccc gtggatcaac ccgacccgga
      361 ggtcgggcaa caaggcagtg agacgctcga actcggcgac agcggaggcc gcctctatct
      421 tggggctgtc ttcgacgagc ggacagacga cgaaagcctg ccgaccttca gccaccgctt
      481 cgccaacctc gtgccatgcc agggactcct gtaacggcga caacaccgtc gtcttcaccg
      541 gagtccgacc tggcggcatc tcgccgatga tcgacatgtc gagatccccg tagatagtca
      601 tcgagagggt tcggggaatc ggggtcgcgg tcatgatcag aaggtcaggg tccaccgaat
      661 cggccttctc cttgagcagc acccgctgcg ccaccccgaa gcgatgctgc tcgtcaacca
      721 cagccacccc gagtcgtgaa aagtggactc cttcctgaat cagcgcatga gtgcccacca
      781 ggatgtccac ctggccattg gccacgtcgg ccagcagctg ttcacggccg atgtcgtccc
      841 gatagttcgc ccgtgccgtc gatcctgtga gcagcgccat gtggaccgca gggccatctg
      901 tggcgaacaa gctggccatt cccaggtccg acatcgggcc gagcgggtcg ggtgagagcc
      961 cggcctcggc cagcagggcg gtgaccgact gatagtgctg accggcaagt acctcagtcg
     1021 gcgccatgat cgctccctgc cacccgccct ccacgccctg aaggaggctg gcaaccgcga
     1081 ccacggtctt gcccgatccc acctcgccct ggagcaatcg gtgcatgggg tgcccgaggc
     1141 gcaggtcgcc tgagatctcc tccagcaccc ggatctgggc ggcggtgaga gtgaatggca
     1201 gacctccgag gaagcgatcc accaactccc cggtgggctg atgggcgatc ccggtcgcgt
     1261 ccagaacctg acgccgcttg cggagcgcca gcgtcagctc gagtcggtag agctcgtcga
     1321 aggcgagtcg ggcccgggcc ggtccgacct gatccatggt ctccgggaag tggatgtgct
     1381 cgtaggcctg atctcgatcg atgagggaca gccgctcgag ggactccgca gggatcgtgt
     1441 ccaggatcgg cttcgagcgc tgtagagcgt tgtggatggc tcgccggagg acgcccggtt
     1501 tgatctggcc gatccgttga tggatcggga cgacgcgccc ggtgcgcagc gactccagcg
     1561 acgagtcgag gaagtcggct tgtgggctct tcatctggag cccaccgttg aaggactcaa
     1621 ccacccccga cagcgcaacc tgggcaccct cgaccagctg cttcgcccta aacggctggt
     1681 tgaaccaaac agccttcatc gaggcgctgt cgtcgaagat gatcgcctcg acgatcatca
     1741 gctttcgctt tgggatgcgt ctcgtcgtca ccgagcgaac ctcgccgacc acagtgacct
     1801 cggtgccacg agatgcctcg gcgatcgact cgttcaatga gcggtcgata tatcggcgag
     1861 ggacatgatg aagaaggtcg gcgacagtca cgatcccggc cttggccagg accggggtac
     1921 gacttccgcc gagcgtcttc acttcagcga catcgacttc tgtgagccgg ctcagagtgg
     1981 ccatacgacc cgggcaacgg tacaaccgga gtgggacttg gtcgggttcg acgctaatct
     2041 gcgctccggt cgcggcggct caccggatcc gcctgacatt ccgacctttc ccaggagttc
     2101 ttagacatgt cttatcgctg cgagatctgt ggcaaggagc cgagctacgg caagaccgtc
     2161 agcttctccc acaagcgcaa caaccgtcgg tggctgccca acatccagcg ggtgcgggta
     2221 cgtcacggat ccaacacacg gcgtgcccgg gtctgcacct cgtgcatccg agccggcaag
     2281 gtggaaaagg cctgaagatg ctgcagggtg tggtcaagac ctacgaccct gtgaccggtg
     2341 ccgggatcat cgttcgtgat gacgaccgtt ccgaggtcta tctccgcccg ggagccctcc
     2401 agggatcgat cttccgaatg ctgcgccagg gccagcgcct tgtcttcgac accgtcgagg
     2461 aggatggcca tcaatacgcc atctcagtga gggtcggcca agagggttac tgagacgtca
     2521 tacgtcgtac gtcatacgtt gtacgcaaga catcggaccc attacctcgt tattgcacct
     2581 ggcttccatg tgtcgctcaa ccgcgataga tgagtctgga tgcaggactt caggaaactg
     2641 gaggtatgga acgcaagtgt tgctctcgct gagaacatct atcggatgac cagccgcttc
     2701 ccgtcggcgg aacgcttcgg tttgagatcc caactgcgat cagcagcggt gtcgatcagt
     2761 tccaacattg cagaaggcgc aggccgtagg accaattcag atactgcaca cttcctccag
     2821 atcgcgatcg gatcctcatg tgaagttgag agccaggtac ttgtatcgaa tcggctcggc
     2881 ttgctaccag atccgggtga actcctcgac gagatcg
//
LOCUS       DBNHXU010000120         5053 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3609962, whole genome shotgun sequence.
ACCESSION   DBNHXU010000120 DBNHXU010000000
VERSION     DBNHXU010000120.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5053)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5053
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3609962"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..115)
                     /locus_tag="AC161X_03150"
     CDS             complement(<1..115)
                     /locus_tag="AC161X_03150"
                     /EC_number="1.17.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017203108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046429 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate synthase activity (ferredoxin) [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin-dependent
                     (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase"
                     /protein_id="HIR4988577.1"
                     /translation="MTPRRLTRQLHVGKVAVGGDAPVSVQSMTTTKTADVDG"
     gene            complement(123..1238)
                     /locus_tag="AC161X_03155"
     CDS             complement(123..1238)
                     /locus_tag="AC161X_03155"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF014244.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HIR4988578.1"
                     /translation="MLGGLAAAIAIIVFVTAHEAGHFFAAKAVGMKTTQFFFGFGPKI
                     WSTQRGETEYGVKWIPLGGYVRIVGMNPLEEVEPGDVGRTYREKKFWEKSIVVLAGVA
                     MNFLIAFVIFYGIAVVNGVNEIQPTVDEVIATAKVGDDTVSTPAAEAGLESGDVIVAI
                     GPHQIDEWEDVPPALDSVGGGPTTVTVERDGETLILDVDMIELEKDDGSGTTGFLGVR
                     PLIENQEIGPIAAIGVAGPQLWDGIGFTFKALGDMLKPSSLAQYIGVFGGGEVDDEIR
                     PVSPIGIVNIGTQAPSTGAFLGILAFVNVILATINLLPLFPLDGGHFAVAVYEKLTGR
                     EANVRRLAPVAAAVIAIFAFLGFVAIILDIVDPISLS"
     gene            complement(1269..2414)
                     /gene="dxr"
                     /locus_tag="AC161X_03160"
     CDS             complement(1269..2414)
                     /gene="dxr"
                     /locus_tag="AC161X_03160"
                     /EC_number="1.1.1.267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013676771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0030604 - 1-deoxy-D-xylulose-5-phosphate
                     reductoisomerase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019288 - isopentenyl diphosphate
                     biosynthetic process, methylerythritol 4-phosphate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HIR4988579.1"
                     /translation="MTRPVVVLGATGSIGSQTLEVADILGFEVAGLAAREPSSELLQL
                     AVRYPNASIAVAGGSGDDRKSFEHDLGRPVMFDNDAVTELARTPGSIVVNGIVGAAGL
                     RATVAGLEAGNRVALANKESMVAGGAVVKTALASHGGELIPVDSEHSALMQCLAGESP
                     ESVARLMLTASGGPFRGRDRDSLADVTPEEALRHPTWDMGRRISIDSATLFNKGLEVI
                     EAHYLFDIAFESIDVVVHPQSILHSAVEFVDGSWKGHFGRPDMRIPIQYALTAPDRAP
                     TPVQPFSMAGLSLTFEEPDRVTFPAIDIAFAAGSQGGSSPAVLNAADEVAVEAFLQRR
                     LGFLGIAEVVARTLEAVPWREVADVDAVLSADVEARATAAGFIAGAC"
     gene            complement(2411..3607)
                     /locus_tag="AC161X_03165"
     CDS             complement(2411..3607)
                     /locus_tag="AC161X_03165"
                     /EC_number="2.7.7.41"
                     /inference="COORDINATES: protein motif:HMM:NF013327.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004605 - phosphatidate
                     cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0016024 - CDP-diacylglycerol biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HIR4988580.1"
                     /translation="MSDRTDSEQEKGFRRPWESEDSEGGVDADADVFTLPEPEPTAAS
                     DVDLTDWEAMTAETGALDEYTAEDYAAATTEEYRGLAEEVSRAAGEEWEQQAVAASMP
                     GVDSGLVGFEDVTGATADSEEDYEAREQAASSDLAMRAGSAVVIFGLFLGSLFLGGWW
                     FTAFVILVMVVAAGEFYATVRHDGYRPLSLFGLLGVILMGIGAHASGVTAIGGWAAVI
                     AAGSVLFFSLSPRRQPLEDAAVTVGAMAWVGLLSFAILIAAGPRPLEYILFTVLLVAA
                     NDTGAYFVGRAFGRRKLAPVISPQKTVEGFFGGLIAGLVVASILVTFPGWEDIGLAKG
                     LVAAALVGIVAPIGDAAESMIKRALDVKDMGSVMPGHGGILDRIDSFLFAVPAIYFLF
                     RGFGLL"
     gene            complement(3604..4155)
                     /gene="frr"
                     /locus_tag="AC161X_03170"
     CDS             complement(3604..4155)
                     /gene="frr"
                     /locus_tag="AC161X_03170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018220709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HIR4988581.1"
                     /translation="MISDLLTEAEHKMEMAIDHVAAEFSTVRTGRANPQLLQRIYVDY
                     YGTPTPLQQLASIAVPEPRLLVVNPFDRSTVSDIERAIQQSDLGLNPTNDGSVIRIAF
                     PPLTEERRRDLIKVVKHMAEEGRVAVRNIRRHSKTDMEAMHGEISDDDIRRGEDGLQK
                     LTDRFVVKIDHLLANKEGELLEV"
     gene            complement(4169..4882)
                     /gene="pyrH"
                     /locus_tag="AC161X_03175"
     CDS             complement(4169..4882)
                     /gene="pyrH"
                     /locus_tag="AC161X_03175"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006910561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HIR4988582.1"
                     /translation="MTDRPRRVVLKLSGEAFADPSVGYGIDPATVIRVAGEVSAAHAT
                     GVQIAVVVGGGNIFRGVSQAAKGMDPASADYMGMLATVINSLALRDALEKQGVPTRVQ
                     SAITMQELAEPYIRLRAIRHLEKGRSVIFAAGTGNPFFTTDTTAALRAVEIGADVLLK
                     ASKVDGIYDADPATHADAIRMAELSYMDFISRELRVMDTTAVTMCKEHGVPIVVFDMF
                     APGNILKAVNGERIGTVIH"
BASE COUNT          966 a         1659 c         1535 g          893 t
ORIGIN      
        1 tgccgtcaac atcggcggtc ttggtagtgg tcatcgactg aaccgagacc ggtgcatccc
       61 cgcccaccgc aaccttgccc acatggagtt gacgagtgag ccggcggggg gtcatcgggt
      121 gctcaagaca acgagatcgg atcgacgatg tcgaggatga tcgccacgaa accgaggaag
      181 gcgaaaatgg cgatgaccgc ggccgccacc ggggccagcc gacggacgtt tgcctccctc
      241 ccggtgagtt tctcgtatac cgccaccgcg aagtggccgc catcgagcgg gaagagggga
      301 agcaggttga tggtggccag aatcacgttg acgaaggcca ggatgccgag gaaggcgccg
      361 gtcgatgggg cctgagttcc gatgttgacg atgccgatcg ggctcaccgg ccggatctcg
      421 tcgtcgacct cgccaccacc aaaaacgccg atgtactggg ccaggctcga tggcttgagc
      481 atgtcaccga gggctttgaa agtgaagccg atcccatccc acagctgtgg gccggccacc
      541 ccgatggctg cgatcggacc aatctcctga ttctcgatca ggggcctgac gccgaggaac
      601 ccggtggtgc ctgagccatc atccttctcc agctcgatca tgtcgacatc gagtatgagg
      661 gtctcaccgt ctcgttcaac tgtgactgtc gtcggaccgc cgccgaccga atcgagtgcc
      721 ggcggcacgt cctcccattc atcgatttgg tgggggccaa tggcgacgat gacgtcgccg
      781 ctctccaaac ccgcctctgc cgccggggtg gagacggtgt cgtcaccgac cttggcagtg
      841 gcgatgactt cgtccacggt cggttggatc tcattgaccc cgttgacgac ggcaatcccg
      901 tagaagatga cgaaggcgat caggaaattc atcgcaacgc cggcgagcac cacgatgctc
      961 ttctcccaga acttcttctc gcggtaagtc ctgccgacat cgccgggctc gacttcttca
     1021 agggggttca tgccgacgat ccgcacatag ccgccgagcg ggatccattt caccccgtac
     1081 tccgtctcac cacgctgcgt cgaccagatc ttggggccga acccgaagaa gaactgggtg
     1141 gttttcatcc cgacggcctt ggccgcgaag aagtgccccg cctcgtgggc ggtgacaaac
     1201 acgatgattg cgatggctgc tgccaaccct ccgagcacgg tgacacgcta cccgggccga
     1261 ccgtttagtc agcaagcacc cgcaatgaat ccggccgctg tggcccgagc ctcgacgtcg
     1321 gccgagagga ccgcatcgac gtcggccacc tctcgccagg ggaccgcttc cagggtccgg
     1381 gccaccacct cggctatgcc gaggaagcca agacgccgct ggagaaatgc ctcgacggcc
     1441 acctcgtcgg cggcattgag caccgctggg ctcgacccgc cttgggatcc ggcggcaaat
     1501 gcaatatcga tggcagggaa ggtgactcga tccggctcct cgaaagtcaa ggagaggccg
     1561 gccatcgaga aaggctgaac aggtgtcggt gcccggtccg gggcggtgag cgcgtattgg
     1621 atcggaatcc tcatatcggg acggccgaaa tggcccttcc acgagccatc gacgaattcc
     1681 accgcggagt ggaggatcga ttgggggtgg accaccacat cgatcgattc gaacgcgatg
     1741 tcgaagagat agtgggcctc gatgacctcc aggcccttgt tgaagagcgt tgcggagtcg
     1801 atcgagatcc gtcggcccat gtcccacgtc ggatgacgca acgcctcctc aggggtgaca
     1861 tccgcaagcg agtcgcgatc acgcccccgg aacggcccgc ccgaggcagt gagcatgagc
     1921 cgcgccaccg attcagggct ctcgccggcc aggcattgca tcagagctga gtgctcgctg
     1981 tcgaccggga tcaactcccc gccgtggcta gccagtgcgg tcttcaccac cgctccgccg
     2041 gccaccatgc tctccttgtt ggcgagtgcg acccgattgc cggcttcaag cccggccacc
     2101 gtggcgcgaa ggcccgcggc gcccacaatc ccgttgacca cgatcgaccc aggggttcgc
     2161 gccagctcgg tcaccgcatc gttgtcgaac atgaccgggc gccccaggtc gtgctcaaag
     2221 ctcttccggt cgtcgccaga cccgccggca acggcgatcg atgcatttgg atagcgaact
     2281 gcgagctgga gcaattcgga ggagggctca cgcgccgcca gaccggcgac ctcgaacccg
     2341 agaatgtccg cgacctccaa cgtttgggag ccaatggagc cggtcgcccc gaggacgacc
     2401 accggccggg tcacagaagc ccgaaacccc ggaacaggaa gtaaatcgcg ggaaccgcaa
     2461 acaagaagga gtcgatgcgg tcgagtatcc ccccatgtcc gggcatgacc gagcccatgt
     2521 ccttgacgtc cagagctcgc ttgatcatcg actccgcagc gtccccgatg ggtgcaacga
     2581 ttccgacgag tgcggcagcc accaatccct tggcgaggcc gatgtcctcc catcccggga
     2641 aggtgaccag gatcgaagcc acgaccagcc cggcgatcag gcctccgaag aagccttcga
     2701 ccgtcttctg gggagagatc actggcgcca gcttccgccg tccgaatgct ctccctacga
     2761 aataggcgcc ggtgtcgttg gcggcgacca gcagcaccgt gaacaggatg tactcgagcg
     2821 gacggggccc ggcagcgatc aggattgcaa acgagaggag gccaacccac gccatggcac
     2881 cgaccgtcac cgccgcatcc tccagcggct ggcgccgggg tgacagggaa aagaacagga
     2941 ccgatcctgc cgcgatcacg gccgcccacc cgcctatggc ggtgacacct gaggcatgcg
     3001 caccgatccc catcaaaatc actcccagga gaccgaacag cgacagcggg cggtatccat
     3061 cgtgtcggac ggtggcgtag aactcgccgg cggcaaccac catcaccagg atcacgaagg
     3121 cggtgaacca ccagccgccc agaaacagac ttcccaggaa cagcccgaag atgacgaccg
     3181 ccgagccggc cctcatcgcg aggtctgagc tcgccgcctg ctctcgggcc tcgtagtcct
     3241 cttcactgtc ggccgtggcc ccggtcacat cctcgaagcc gaccagcccg ctgtcgacgc
     3301 caggcatcga ggccgccacc gcttgctgct cccattcttc tccggcggcc cggctcacct
     3361 cttcggcgag gccccgatac tcctcagtgg tggcagccgc gtagtcctcg gcggtgtact
     3421 cgtccagggc gccggtttct gcggtcatcg cctcccagtc ggtgagatcg acgtcactcg
     3481 cagcagtggg ctcaggttcg ggaagggtga acacgtcggc gtcggcatct accccaccct
     3541 cggaatcttc agactcccaa ggccgccgga aacccttctc ctgctcgctg tcggtccggt
     3601 cgctcatacc tcgagcaatt ccccttcctt gttggcgaga aggtggtcga ttttcaccac
     3661 gaaacgatcg gtgagtttct ggagcccatc ctcacctcgg cgaatgtcat cgtccgagat
     3721 ctctccatgc atcgcctcca tgtcggtctt cgagtgacgc cgaatgttac ggactgcgac
     3781 ccttccttcc tcggccatgt gcttcacgac tttgatgagg tcccgacgcc gttcctcggt
     3841 tagcgggggg aatgcgatcc gaatcaccga accgtcgttg gtcggattga gcccgagatc
     3901 ggactgctgg atcgcccgtt cgatatcgga aacggtcgat cgatcgaagg ggttcacgac
     3961 gagaagcctg ggctcaggca ctgcgatgga tgccaattgc tgcaacgggg tgggagtccc
     4021 gtagtagtcg acatagatcc gttgcaggag ctgggggttg gctcgaccgg tcctcaccgt
     4081 ggagaactcg gcagcgacat gatcaattgc catctccatc ttgtgctcgg cctcggtcaa
     4141 gagatcgctg atcatcgccc tcacctcctc agtggatcac cgtgccgatt cgctcgccgt
     4201 tgacggcttt caggatgttg cctggggcga acatgtcgaa gaccacgatc ggcactccgt
     4261 gctccttgca catcgtaacg gcggtcgtat ccatcacccg gagttcacga gagatgaagt
     4321 ccatgtagga gagctcggcc atgcgaatgg cgtccgcatg ggtggcgggg tcggcgtcat
     4381 agataccgtc gaccttgctc gccttgagca gaacgtcggc gccgatctcc acggcacgca
     4441 acgcggcggt ggtatccgtg gtgaagaacg ggttgccggt cccggcggca aagatgacgg
     4501 agcgcccctt ttcaagatga cggatggccc gcagccttat gtaaggctct gccagctcct
     4561 gcatcgtgat cgcactctgg acccgggtgg ggacgccctg cttttcgaga gcatccctca
     4621 gggccagcga gttgatgacc gtggccaaca tccccatgta gtcggccgaa gcgggatcca
     4681 tccccttcgc cgcctgggag acgccgcgga agatgtttcc tcctccgaca accactgcga
     4741 tttggacccc ggtcgcgtgc gcggctgaaa cctcaccggc cacacggatc actgtcgccg
     4801 ggtcgatgcc ataaccgaca ctcggatcgg caaaggcctc accggagagc ttgaggacta
     4861 cgcgtcgcgg cctgtctgtc acttcgctct cctactcgcc aacggctacc cgcgcgaact
     4921 tgcgcaccga gatgttctca cccatcttcg ccgcgagttg ggagacaagg tcgccaacgg
     4981 tcccttcgaa tttctcggcg ttcacaaatt tctggtcgta gagcactttg tccgtgtacc
     5041 aggagttgag cct
//
LOCUS       DBNHXU010000121         3946 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_435847, whole genome shotgun sequence.
ACCESSION   DBNHXU010000121 DBNHXU010000000
VERSION     DBNHXU010000121.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3946)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3946)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3946
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_435847"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1501)
                     /locus_tag="AC161X_03180"
     CDS             complement(<1..1501)
                     /locus_tag="AC161X_03180"
                     /inference="COORDINATES: protein motif:HMM:NF024753.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamB
                     family protein"
                     /protein_id="HIR4988583.1"
                     /translation="MNERFGQVLLVLVIGLVLGLGVFLVTRDNAAPVPGDGTTPQAGA
                     TTTTPGNGSGSTVPGETTTSSPGGTQAPGERPADTVPGSTFGHPWGTTVGLTMFRGNP
                     TRSFFGTGDLSQEPSVKWTYPESAMCSTSTNLGETTQWCGMGWTGQPVVWERPDGITE
                     LIFGAYDSAVHFVNAETGEDLMPKFQTGDLIKGSVSLDPDGYPLLYFGSRDNKYRILS
                     LDGEEAVELWSMDALDVPGTWNDDWDSNGAVVDDILYEGGENSWFFAVELNRGYDSNG
                     KVTVDPEILVQTPGYDDEFIDLSGGNVSIENSPVLYEQRVYFANSGGRIIGLDVSNVR
                     EGEAPVVFDYWAGGDIDASLVVDADGMLYAAVNVKPDQVAPGYRTQANIDRTREVGQL
                     LKLDPYADGDPRLWGVDLTAGSEQAGTWSTPAVYQGVVYNNTMQGSLIAVDAATGEII
                     WSDEVGWHSWSSPAVVDGMLVTATCNGDVRGYSLEDPRSPVRQWTVDLGE"
     gene            complement(1515..2552)
                     /locus_tag="AC161X_03185"
     CDS             complement(1515..2552)
                     /locus_tag="AC161X_03185"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021127.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045227 - capsule polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HIR4988584.1"
                     /translation="MSRSHLLIALMVSSCVGSGVASTTTPTTTTTSTTVLATSTTIMP
                     ATTTTTVPEKEWLVIQGTGDVNLDPSYIPVLAVEGWDHAFSGLEGFLLEDSLTVVNLE
                     CAPSALGSPLDKDFVFRCPPESLGALAAGGVEVASMGNNHSGDYGKEALVDGRANLIA
                     AGVSPIGAGANADEAGSPALFEIGGWRVAVVGFGGVVGDASWFATDDQAGIRDGDDIP
                     SMVEAVEAAAALADLVVVAIHWGVELDTMPRPEDVERGRAMIAAGADIIFGHHPHRLQ
                     PLDVVDGRPVFWSLGNFVWPNFSVAGSTTAVARAVVAPDGTIEACLIPAVIESPGHPV
                     MTAEPNCGPPS"
     gene            complement(2594..3376)
                     /locus_tag="AC161X_03190"
     CDS             complement(2594..3376)
                     /locus_tag="AC161X_03190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015424 - ABC-type amino acid transporter
                     activity [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HIR4988585.1"
                     /translation="MTVEHNHQIFADATRVKGDPIIEVLGVDKFFGEFQALKDVNINV
                     GLQEVVVVIGPSGAGKSTLIRCVNRLEKHDRGRIVVDGIELTDDARTIQEVRRETGMV
                     FQQLNLFPHLTVLENITLAPRHVRHETRIEAEAHGMELLARVGIPEQAQKHPGQLSGG
                     QQQRVAIARSLAMRPKVMLFDEATSALDPEMIKEVLDVMIELAEGGMTMIVVTHEMGF
                     ARAVADRVVFMADSEIVEVGTAEHFFTDPQEERTKLFLSQIL"
     gene            3475..>3946
                     /locus_tag="AC161X_03195"
     CDS             3475..>3946
                     /locus_tag="AC161X_03195"
                     /inference="COORDINATES: protein motif:HMM:NF012707.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HIR4988586.1"
                     /translation="MKNRYKLLTLAVVLAFVAAACGAEEGSGDVEGNDLGGRNVTVAV
                     ENAYLPFNYILPGETEGQGWDYDAWRHICEVLNCVPEFVETAWEPMIDQVAQGEFDVA
                     ADGISITDERKEVVDYSDAYMVVEQKFIVQLADERYATAQDLIDDTDAIVATQVG"
BASE COUNT          754 a         1250 c         1213 g          729 t
ORIGIN      
        1 tctcaccgag gtcgaccgtc cactgcctca ccggggatct ggggtcttcc agggagtagc
       61 cgcgcacgtc gccgttgcag gtggctgtca ccagcatgcc gtcgacgacc gccggtgagg
      121 accaggagtg ccagcccact tcgtcagacc aaatgatctc accggtcgcg gcgtcgacag
      181 cgatcaggct tccctgcatg gtgttgttgt aaacgacccc ctgatagacc gccggcgtgc
      241 tccaggttcc cgcctgctcg ctgccggcgg tgagatcgac gccccaaagc ctcgggtcgc
      301 catcggcata ggggtcgagt ttgagcaact gccccacttc ccgggtgcgg tcgatgttgg
      361 cctgggttcg atacccgggt gcgacctgat ccggcttcac attcacagcc gcatagagca
      421 tcccgtcggc gtcgaccaca agcgaagcgt cgatatcgcc cccggcccaa tagtcgaaga
      481 cgaccggcgc ctcgccctca cggacattgc tcacatcgag gccgatgatc cgaccgccgg
      541 agttggcgaa gtagacccgc tgctcataga ggaccggcga gttctcaatg gagacgtttc
      601 ctcccgagag gtcgatgaat tcgtcgtcgt atccaggggt ctggaccaag atctctgggt
      661 cgactgtgac cttcccattg gagtcgtagc cccgattgag ctcgacggca aagaaccagc
      721 tgttctctcc accctcatag aggatgtcgt cgaccacggc cccgttggag tcccagtcgt
      781 cgttccaggt gccgggaaca tcgagagcgt ccatcgacca cagctcgacc gcctcctcgc
      841 cgtccaggga gaggatccgg tacttgttgt cccgggagcc gaagtagagc agtggatagc
      901 cgtcagggtc cagggatacc gagcccttga tcaggtcccc ggtctgaaac ttcggcatga
      961 ggtcctcgcc tgtttccgca ttcacgaagt gaaccgccga gtcgtaggcg ccaaagatga
     1021 gctcggtgat cccgtcaggc cgctcccaga ccactggctg accggtccag cccattccac
     1081 accattgagt ggtctctccg aggttggtcg aggtgctgca catcgcactt tccgggtatg
     1141 tccacttgac cgacggctcc tggctgaggt cgccggtgcc gaaaaagctc cgtgtcgggt
     1201 taccgcggaa catggtcaaa cccacggtgg tgccccacgg atggccgaat gtggaaccgg
     1261 ggactgtgtc ggctggccgc tctccgggag cctgggtccc gccaggggac gacgtcgtgg
     1321 tctctccggg cacggtggac ccggacccgt ttcccggggt cgttgtggtc gctcccgcct
     1381 gcggtgtcgt gccgtcaccc ggcaccggag cagcattgtc tctggtgacc aggaacacac
     1441 cgaggcccag taccaagccg atgacgagga ccagcaagac ctggccgaag cgctcgttca
     1501 tttcagatcc aacgtcagga aggtggaccg cagttcggct cggcggtcat caccggatgc
     1561 cctgggctct cgatcacggc cgggatgagg catgcctcga ttgtcccatc gggtgccacc
     1621 acggcgcggg caaccgcggt ggtcgagccg gcgactgaga aattgggcca tacgaagttc
     1681 cctagagacc agaacacagg tcggccgtcg acgacgtcga gcggctgcag ccgatgaggg
     1741 tgatgcccga agatgatatc ggctcctgcc gcaatcattg cccgaccccg ttccacgtcc
     1801 tcggggcggg gcatcgtgtc cagctccacg ccccagtgga tggccacgac gacaagatcg
     1861 gcgagcgccg ccgccgcctc gaccgcctcc accatcgagg gaatgtcgtc accgtccctg
     1921 atgcctgcct ggtcgtcggt ggcaaaccac gaggcatcgc caaccacgcc cccgaagccg
     1981 acaaccgcca ccctccaacc tccgatttcg aaaagagccg gggacccggc ctcgtcggcg
     2041 ttcgctccgg ctccaatcgg gctaaccccg gccgcgatca gattggcgcg accgtcgacc
     2101 aatgcctcct tgccgtagtc gccggagtgg ttgttgccca tgctggctac ctccaccccg
     2161 cccgcggcga gcgcgccgag ggattcgggg gggcagcgga acacaaagtc cttgtcgaga
     2221 gggctcccca gggccgacgg agcacattcc agattgacca ccgtaaggct gtcttcgaga
     2281 agaaagccct cgaggccgga gaaggcgtgg tcccatcctt cgacggcaag cactgggatg
     2341 taactgggat cgaggttgac gtccccggtc ccctgaatga ccagccactc cttctccgga
     2401 accgtcgtgg tggtggtcgc cggcatgatg gtcgtagacg tggcgagcac ggtggtcgat
     2461 gtggtcgtcg tggtgggagt agtggtcgac gcgacccccg acccgacaca actcgagacc
     2521 atcagtgcga tgagcaggtg tgatcgagac aagaaacgcc agcgtacgac gtatgacgta
     2581 tgacgtaatg cggctagagg atctgactca agaagagctt ggttcgctcc tcctgggggt
     2641 cggtgaagaa atgctcggca gttcccacct ccacgatctc ggagtccgcc atgaacacga
     2701 cccggtcggc cacggcacgt gcgaatccca tctcgtgggt gacgacgatc atcgtcatcc
     2761 caccttcggc cagctcgatc atgacgtcga gcacctcttt gatcatctcc ggatcgagag
     2821 cggaggtcgc ctcgtcgaac agcatcacct ttggcctcat cgccagggaa cgggcgatgg
     2881 cgacccgttg ttgctggcca ccggaaagct gacccgggtg cttctgagct tgctccggga
     2941 tcccgaccct tgccagaagt tccatcccgt gggcctccgc ctcgatcctg gtctcgtggc
     3001 gcacgtgacg tggagcgagg gtgatgtttt ccaacacagt gagatggggg aacaaattga
     3061 gctgttgaaa gaccatgccg gtctcccggc gcacctcctg aatggtgcgt gcatcatcgg
     3121 tgagctcaat gccgtcgacc acgatccgcc cccgatcgtg tttctccaat cggttgacgc
     3181 aacggatcag agtggatttg ccggcgccgg agggcccgat gaccaccact acttcctgca
     3241 ggccaacatt gatgttgacg tccttgagcg cctggaactc gccaaagaac ttgtcgacac
     3301 cgagaacttc gataatcgga tcgcctttga cccgggtggc gtcggcgaaa atctgatgat
     3361 tgtgttcaac ggtcataacc acatccgacg cttcggtcgg gaacgcgacc ctagcccgac
     3421 tgcccaatct tgatacgctt caggcggctc tgagagcctg aaaggaagaa acatatgaag
     3481 aaccgataca aactattgac gcttgccgtg gtcctggcct tcgtggccgc cgcttgcggc
     3541 gcagaggagg gctcaggcga cgtcgagggg aatgacctcg ggggcaggaa cgtcacggtt
     3601 gccgtagaga acgcctacct cccgttcaat tacatactcc ccggtgagac cgagggtcag
     3661 ggctgggact atgacgcctg gaggcacatc tgtgaggtcc tcaactgtgt cccagaattc
     3721 gtcgagaccg cctgggagcc catgatcgac caggtggctc agggtgaatt cgacgtcgcc
     3781 gccgacggga tctcgatcac cgatgagcgc aaggaagtcg tcgactactc cgatgcctac
     3841 atggtggtgg agcagaagtt cattgttcag ctcgctgacg aacgctacgc gactgctcag
     3901 gatctgatcg acgacacgga tgccatcgtg gccacccagg tgggaa
//
LOCUS       DBNHXU010000122         6706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1428384, whole genome shotgun sequence.
ACCESSION   DBNHXU010000122 DBNHXU010000000
VERSION     DBNHXU010000122.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6706
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1428384"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..435
                     /gene="nrdR"
                     /locus_tag="AC161X_03200"
     CDS             <1..435
                     /gene="nrdR"
                     /locus_tag="AC161X_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013156598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HIR4988587.1"
                     /translation="CGAPDTRVIDSRPAEGGQSIRRRRSCETCDGRFTTYERLEPQLM
                     VRKRNGRLEAFSSAKLAAGISAAFADRPVSGSAIESIVAEVEHSLRHEGSPVTSEDIG
                     HQVLAQLRAVDEVAYLRFASVYKDFQDAADFEKEVAALESSE"
     gene            complement(424..1434)
                     /locus_tag="AC161X_03205"
     CDS             complement(424..1434)
                     /locus_tag="AC161X_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988588.1"
                     /translation="MTATSPTKHGRFARFPSMESLGFSDAPSETTVVYNAPSQLSTTT
                     TVITRPTAPTGHLVQDLWDSTELDAPSLVDWKPISPDRRHLKARRFRWSTVLLLLVVA
                     VAAGGTAFWLYQRSSGAAAASVAAVQEQATQLSASLDGIGPIADQLAAPASTVNLTAT
                     DLFDLDDAARGLFDASVALPDPETDTETIAGDAASLSFEIVRQLRDGLAYRGALEPML
                     VAPALETDPTLTDMATATFEFSKWRSEFDNVRAALPEGVAATVTFAVDEFSAGLEAAQ
                     GTYLDAIRNDDRAGAEEALQALESDLDSIRGLMLTTMGGLAGQVGSELEETRRLLDRL
                     LG"
     gene            1474..1917
                     /gene="dut"
                     /locus_tag="AC161X_03210"
     CDS             1474..1917
                     /gene="dut"
                     /locus_tag="AC161X_03210"
                     /EC_number="3.6.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015786733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004170 - dUTP diphosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006226 - dUMP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0046081 - dUTP catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HIR4988589.1"
                     /translation="MPRDTMQVNFVRLDPELEIPTRSHIGDAAIDLHSRIDFGLAPGE
                     RAAVPTGIAVAIPEGFAGLVLPRSGHALRSGIGVVNGPGLIDSGYRGEVTVLLINHGA
                     EQVDFSRGDRVAQLAVVPIPDVEWTEVEDLDATVRGDGGFGSTGV"
     gene            1968..2041
                     /locus_tag="AC161X_03215"
     tRNA            1968..2041
                     /locus_tag="AC161X_03215"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2002..2004,aa:Gly,seq:gcc)
     gene            2076..2146
                     /locus_tag="AC161X_03220"
     tRNA            2076..2146
                     /locus_tag="AC161X_03220"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2108..2110,aa:Cys,seq:gca)
     gene            2210..2344
                     /locus_tag="AC161X_03225"
     CDS             2210..2344
                     /locus_tag="AC161X_03225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988590.1"
                     /translation="MLLGEQLTPRQWMGAVVIVLAVTVAARSVAGRTTEAPDPSLTDA
                     "
     gene            2481..3218
                     /locus_tag="AC161X_03230"
     CDS             2481..3218
                     /locus_tag="AC161X_03230"
                     /inference="COORDINATES: protein motif:HMM:NF024803.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR4988591.1"
                     /translation="MNGYKISEAANRTGFSESALRFYEQQGVVVPERTSSGYRSYNAE
                     DIESLQFLARAKRLGLTLDEISELLLLLDDDECRPVQDRMRALVTERIDETQAKIGDL
                     IAFTGQLQRAAARLGPHTPDGGCDDDCGCKSDSPAVTTRTLIPLGASQDISCSIEPGQ
                     AVERIEDWKALLAQAVGRQPIPSGIRISFTRDVDIAGLSQLAASEQTCCQFFNFTIGI
                     ASDTVSLDVTGPGDAQPVIASIFGVAA"
     gene            3215..3457
                     /locus_tag="AC161X_03235"
     CDS             3215..3457
                     /locus_tag="AC161X_03235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988592.1"
                     /translation="MKRGEIIAFAAACLACCLPLLLGIIGVTTGATGAVGFWVGRNQA
                     LIVASIGLAYLVYTAARHIRAKASNVEKVDLTRKPS"
     gene            3471..3809
                     /locus_tag="AC161X_03240"
     CDS             3471..3809
                     /locus_tag="AC161X_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YnfA family protein"
                     /protein_id="HIR4988593.1"
                     /translation="MTVARSIGLFIIAAIAEIGGAYLVWQGVREDKGWYYVAAGIVAL
                     ALYGFVATFQPSPHFGRVLAAYGGVFVAGSLVWGMVFDGFRPDRYDTIGAALCLIGVG
                     VIMFSPRTLS"
     gene            complement(3872..4234)
                     /gene="crcB"
                     /locus_tag="AC161X_03245"
     CDS             complement(3872..4234)
                     /gene="crcB"
                     /locus_tag="AC161X_03245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014079399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:1903425 - fluoride transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:1903424 - fluoride transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HIR4988594.1"
                     /translation="MRFLWVGLGGAFGSMLRYAIGLAFAASTFPVATLVVNVTGSFVI
                     GLVFSAFLGRWPVPVMTAITVGVLGGFTTFSTFAWESLSAAQDDRLGMAAGYVAASVM
                     GGLFAVWLGYLAGRAITA"
     regulatory      complement(4255..4325)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF01734"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Fluoride riboswitches increase expression of
                     downstream genes that help mitigate the toxic effects of
                     very high levels of fluoride; They are found in a wide
                     variety of bacteria and archaea; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="fluoride"
                     /db_xref="RFAM:RF01734"
     gene            complement(4428..5630)
                     /locus_tag="AC161X_03250"
     CDS             complement(4428..5630)
                     /locus_tag="AC161X_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4988595.1"
                     /translation="MTKGNREGWLTPGVRGIGGASLLADLGHEVPTSLLPTLLTSTLG
                     APAAALGLIEGVADAGAGIAKYGGGLLADDPIRRRSVAVGGYTATAIFSSLIGVATSV
                     WQVAVLRVGAWVARGIRVPARNALLADLTGPGTYGRAYGFERMMDNLGAVGGPLLALG
                     LVAAFGLRGAFITSGVFGLAATGSMIYAIRHIERPMKRERVPIRIRIRPLLVGDLGRL
                     WVPIAGFEVGNLATTLLILRATELLTPGADLESATRTALLLYAAHNLAATLVAIPAGR
                     AADRRGFRPVLTIGFVFGLAAYLLFGITGAGLVTLAVAFVLAGITIGLVETAENGAVA
                     EAAPEELRGSAFGFLAAIQSFGNLIASGVAGLLWTFVDPLAAFVFAAAGMVVALVGTV
                     VLRRASVS"
     gene            6106..6180
                     /locus_tag="AC161X_03255"
     tRNA            6106..6180
                     /locus_tag="AC161X_03255"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:6141..6143,aa:Val,seq:gac)
     gene            6304..6693
                     /locus_tag="AC161X_03260"
     CDS             6304..6693
                     /locus_tag="AC161X_03260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988596.1"
                     /translation="MSQEHKDALARGRKESRAIKAYLKTIESRKPGRPVTKDSLNSRL
                     ATVNNKLQSTDDPLKRLDLLQSRLDIEEALESVEEVANLDQIEADFVANASAYSQRKG
                     ISYTAWRRFGVPAVVLKKAGIPETRRR"
BASE COUNT         1338 a         2153 c         2091 g         1124 t
ORIGIN      
        1 tgtggcgcac ccgacaccag ggttatcgac agtcgaccgg ccgaaggcgg tcagtcgatt
       61 cgtcgtcgtc ggtcgtgcga gacctgtgac ggcagattca ccacttacga gcgcctcgaa
      121 ccccagctga tggttcgcaa gcggaatggc cgactggagg ccttttcttc cgccaagctg
      181 gctgccggga tctcggcagc attcgccgat cgcccagtct ctggttccgc tatcgagtcg
      241 atcgtcgccg aagtcgagca ctcactccgt catgaggggt cgccggtgac ctcagaggac
      301 atcgggcatc aggtcctcgc tcagctcaga gctgtggacg aggtggccta tctccggttc
      361 gcctcggtgt acaaggattt tcaggacgcc gccgactttg agaaggaagt cgccgccctg
      421 gaatcatccg agtaggcggt cgagcaggcg ccgggtctcc tccaactcag agccgacctg
      481 tccggcgagg ccgcccatcg tggtcagcat caacccccgg atcgagtcga ggtccgattc
      541 gagcgcttgg agagcctcct cggcgccggc ccggtcgtcg ttgcggatcg cgtcgagata
      601 ggtcccctgc gcggcttcga ggccggcgct gaactcgtcg acggcgaagg tgacggttgc
      661 cgccactccc tccggcaggg ccgctcggac gttgtcaaac tctgatcgcc acttcgagaa
      721 ctcgaacgtg gcggtggcca tgtcggtgag tgtcggatct gtttccaagg ccggggcaac
      781 cagcattggc tccagagctc cgcggtaggc aagcccgtca cgcagctgcc gcacgatttc
      841 gaacgacagc gaggcggcat cgcctgcgat cgtctcggtg tcagtctcag gatcgggcag
      901 ggcgacagag gcgtcgaaca ggcccctcgc cgcgtcgtcg aggtcgaaga gatcggttgc
      961 ggtcaggttc acggtgctcg ccggcgcggc gagctgatcg gcgatcggac cgatcccgtc
     1021 caggctcgcc gaaagctggg tggcctgttc ttgaaccgca gccaccgacg ccgccgccgc
     1081 tccacttgaa cgctggtaca gccagaaagc agttccaccg gcagcaactg cgacgaccaa
     1141 gagcagcagc accgtgctcc accggaagcg gcgggctttg agatggcgtc ggtcgggtga
     1201 tatcggcttc cagtcgacaa gcgaaggagc atccagctcg gtggaatccc aaaggtcttg
     1261 gaccaaatga ccggtcgggg cggtcggacg ggtgatgact gtcgtggtgg tcgacaactg
     1321 gctcggcgcg ttatagacga cggtcgtctc gcttggcgcg tcggagaacc cgagcgactc
     1381 catcgagggg aaacgcgcaa acctgccgtg ctttgtaggt gaggtagcgg tcatcgaggg
     1441 ctctcgctta cagtcgtcaa gtcatctcgc ccgatgcccc gtgacaccat gcaggttaac
     1501 ttcgtacgtc tcgacccgga actagaaatt cccacaaggt cacatatcgg cgatgccgcc
     1561 atcgaccttc actccagaat tgactttggc ctcgcgcctg gggagcgagc ggcggtgcca
     1621 accgggatcg cagtagccat tccggaaggg tttgcggggc tggtgctgcc caggagtggt
     1681 cacgcactca ggtccggcat cggcgtggtc aacgggcccg gtctcatcga ctccggctac
     1741 cggggcgaag tgaccgttct cctcatcaat cacggggctg aacaagtcga cttctctcgc
     1801 ggggaccggg tggcccaact ggccgtggtg cccatccccg atgtggagtg gacggaggtc
     1861 gaagacctcg atgcgacggt gcgaggcgac ggaggattcg gctcgaccgg ggtctgaggc
     1921 aaccatttgg ttggcatttg gcccgggcta caatgccgcc tccgctcgcg gaagtagctc
     1981 agttggctag agcgcaacct tgccaaggtt gaggtcgcgg gttcgaatcc cgtcttccgc
     2041 tctgacgacg gacccaggtc aagtcgtccg gtaccggcga cgtggccgag tggttaggcg
     2101 cgggtctgca aaacccgtca caccggttcg attccggtcg tcgcctcgag agaggaacac
     2161 gggctcttag ctcagcctgg ctagagcgct tgctcgacac gacagcagca tgctgctcgg
     2221 cgaacagttg acaccccgac agtggatggg tgccgttgtc atcgtgctcg ccgttacggt
     2281 tgctgcgcgg agcgtcgccg ggcgcacgac cgaggcccct gacccctcac tgacagacgc
     2341 ctgacagatc accgacgctg gtcgacacgg cagcgacggc gtcgccccgc ccgagtggta
     2401 cgacgagcag tacgccgact agtcaagatt ctcggaaaag gcttgacctt caacccgcct
     2461 tgaaggttta ttgtggtggc atgaacggat acaagatttc ggaagcggct aaccggaccg
     2521 ggttctcaga gagcgccctc cgcttctacg agcagcaggg agtggtggta cccgagcgta
     2581 catcaagcgg ctaccggagc tacaacgccg aggacatcga gtcgttgcag ttcctggcaa
     2641 gggccaagag actcggcctc accctcgatg agatcagcga gttgctcctg cttctcgacg
     2701 acgatgagtg tcgaccggtg caggatcgga tgcgagcgct ggtcaccgag aggatcgacg
     2761 agacgcaggc caagatcgga gacctgatcg cgttcacggg acaactccaa cgagccgcag
     2821 cccgtctcgg ccctcacact ccagacggcg gctgcgacga cgattgcggc tgcaagtccg
     2881 actcacctgc ggtgaccacg cggaccctga ttcccctggg agcgtcccaa gacatctcgt
     2941 gcagcatcga acccggccaa gccgtcgaac ggatcgagga ctggaaggcg ttgctcgccc
     3001 aggcggtggg ccgccaaccg atcccctcgg ggattcggat cagcttcacc cgcgacgtcg
     3061 acatcgcggg actgagccag cttgccgcca gcgaacagac ctgctgtcag ttcttcaact
     3121 tcacgatcgg catcgcctcc gacaccgtga gcctcgacgt gaccggccct ggagacgccc
     3181 agccggtgat cgcatccatc ttcggagtcg ccgcatgaag cggggagaga tcatcgcctt
     3241 cgctgcagcc tgccttgcct gctgtctccc cctcctgctc ggcatcatcg gagtcaccac
     3301 cggagccacc ggcgcggtcg gattctgggt cgggagaaac caggccctga tcgtggcctc
     3361 gatcgggctc gcctacctgg tctacaccgc agcccgacac attcgagcga aagcatcgaa
     3421 cgtcgagaag gtcgacctga cccgcaagcc cagctaggta gcgtcctcgg atgaccgtcg
     3481 cgcgatcaat cggactcttc atcatcgctg ccatcgctga gatcggcggt gcctacttgg
     3541 tctggcaggg agtccgagaa gacaaaggct ggtactacgt cgccgcaggc atcgtcgccc
     3601 tcgctctcta tggattcgtc gccaccttcc aacccagccc acacttcgga agagtcctcg
     3661 ccgcctacgg cggagtcttc gtcgcaggct cgctggtttg ggggatggtc ttcgacggct
     3721 tccgacccga ccgctacgac accatcggag ccgccctctg cctcatcggc gtcggagtca
     3781 tcatgttctc gccccgcacc ctcagctgac gcccacgggc tgacctccaa aacggctaac
     3841 agctagacgt cgtctgggac agaccagtcc gctacgcggt gatcgcgcga cccgcgagat
     3901 acccaagcca gactgcgaat agcccgccca tcaccgacgc tgcgacgtac ccggccgcca
     3961 taccaagccg gtcgtcctgt gccgctgaca ggctctccca tgcgaaggtc gagaaggtgg
     4021 tgaacccgcc gagcacgccg accgtgatcg ccgtcatcac cggcaccggc cacctgccga
     4081 ggaacgccga gaagaccagg ccgatgacga acgaccccgt tacgttcacg acgagagtcg
     4141 ctacgggaaa cgtcgaggca gcgaaagcga gcccgatcgc atacctcagc atcgacccga
     4201 atgctccacc caacccgacc cacaggaacc gcatagctac ctccacgctc gatggactca
     4261 gtaggagcca tcagcaggca gcctgcggtt tcatcaacga ggcgggctcc atcaccctga
     4321 agtggaggct agacatgccg agcggccagg agcgaagagc atcgagcagg aagcgcatgg
     4381 cgggtgagat cgggacaccg gacaacggcc cgcaacctac aggtcgacta ggacacagag
     4441 gctcttctca gaaccacagt tccgaccagg gcgacgacca tcccagcggc agcgaagacg
     4501 aacgcggcaa gcgggtcgac gaaagtccag agcaatccgg cgacgccgga ggcgatcagg
     4561 ttcccgaagc tctggatcgc ggccagaaat ccgaaggcgg aacctcgaag ctcttcgggt
     4621 gcggcttcgg caacggctcc gttctcggct gtctcgacga ggccgatggt gatcccggcg
     4681 aggacgaaag ccacagcgag agtgaccagg cccgctccgg taataccgaa taggaggtag
     4741 gcggctagcc cgaagacgaa accgatcgtg agcactggtc ggaacccgcg acgatcggct
     4801 gccctccccg cagggatcgc gaccagagtc gctgccaggt tgtgcgccgc gtagagcagg
     4861 agagcggttc tcgtagccga ttcgagatca gctcccggtg tcagcaattc ggtagctcga
     4921 aggatgagaa gcgtcgtcgc gagattgcca acctcgaatc ctgcgatcgg cacccagagc
     4981 cgacccagat cgccgacgag aagcggacga atgcgaatcc tgatcgggac ccgctctcgt
     5041 ttcatgggac gttcgatatg gcggatcgca tagatcatcg agccggtcgc agcgagcccg
     5101 aagactcccg aagtgatgaa cgcaccccgc aacccgaagg cggcgaccag gccgagagcg
     5161 agcagcgggc caccgacggc tcccaggttg tccatcatcc gctcgaagcc gtatgcccgc
     5221 ccataggtgc cagggccggt caagtcagcg agcagcgcgt tacgagcagg aacccgaatc
     5281 ccgcgagcaa cccaggctcc gacccgaagc accgccacct gccagaccga agtcgcgaca
     5341 ccgatcagcg acgagaagat cgcagtcgcc gtgtagccgc cgaccgccac ggagcggcga
     5401 cggatcggat cgtcggcaag cagcccaccc ccatacttgg caataccggc tccggcatcg
     5461 gcgacacctt cgatcagccc gagagcagca gcgggcgccc ccaacgtgga cgtgagaaga
     5521 gtcgggagca gagacgtcgg aacctcgtga cctagatcag cgagaagact tgccccaccg
     5581 atcccacgaa caccaggagt cagccagcct tcacgattgc ccttagtcac gttgagacca
     5641 gcgtatgagc tacgaggccc ccagggggta cccaccaagg cggccccaga cagaggtcga
     5701 gcgaaactcg ccgcatatgg catcgtcgaa tatcagggtt tcgccctcgt cggcggttct
     5761 agcttcgcgc gagagctcga cgctgcgaga cgacgctgct gaccattctc tcggagcttg
     5821 gcttcagccc gacggggcgc gctcgtctcg ggctcgccgt gatcgaagtc gagcgtcgac
     5881 tctctcaggt cgccgacggt cgattcgcct ctaccggcga attctgagcg cttgcgtggc
     5941 gcggccccga atctctgcgc ccaaattgcg cctgctgcag cgctacttcg tcgacgcgcg
     6001 ctgatactcg ctgacgcgcg agcttccagg ctgtcagtag aaatctctaa gctctacgct
     6061 tggatttcgg cgatcgacgg catcttgcct ggtagtcgaa gagacgggcc cttagctcag
     6121 cctggctaga gcgcttgctc gacacgcaag aggtcactgg ttcaagtcca gtagggccca
     6181 ccgagagaaa accccccgaa caaaaggggg gtttctttcc gttctggcgg tggtttcgtg
     6241 cgaacgcagt tgtgccatta ttggtgtcgt tcgacggaag cattaggaga gtttcgaatg
     6301 gcgatgtccc aggaacacaa agacgccctt gcccgcggcc gcaaagagtc aagggcgatc
     6361 aaggcctatc tcaaaaccat cgagtcgcgt aagccgggcc ggcccgtaac caaggacagc
     6421 ctcaattcca ggctggcaac ggtcaacaac aagctccaga gcacggatga tcccttgaag
     6481 cggttggatc ttctccagtc gaggctcgat atcgaagagg cattggagtc ggtggaggaa
     6541 gtggcgaatc tcgaccagat cgaggctgat ttcgtggcaa atgcatccgc atactcgcag
     6601 cgaaagggca tcagctacac ggcgtggcgt cgttttggag tgccggctgt ggtcctcaag
     6661 aaggccggga ttcccgaaac caggcgccgc tagtcccagc ccaact
//
LOCUS       DBNHXU010000123         3857 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2817069, whole genome shotgun sequence.
ACCESSION   DBNHXU010000123 DBNHXU010000000
VERSION     DBNHXU010000123.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3857)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3857
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2817069"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="AC161X_03265"
     CDS             <1..355
                     /locus_tag="AC161X_03265"
                     /inference="COORDINATES: protein motif:HMM:NF013286.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HIR4988597.1"
                     /translation="VTITSSSAPDRIEIDQEKLAETLEYIRPAVQADGGDLILLGTEG
                     GTVNIQMVGACGGCPLSMMTLKAGIERILIDRVPGVDEVNASADAPALEENVASAESG
                     GITLPGHLMASPDLI"
     gene            535..975
                     /gene="yiaA"
                     /locus_tag="AC161X_03270"
     CDS             535..975
                     /gene="yiaA"
                     /locus_tag="AC161X_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017461835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inner membrane protein YiaA"
                     /protein_id="HIR4988598.1"
                     /translation="MTQTLQRPTTAFVGASWLALLVGMAAFLIGLSNATMDLNEKGYY
                     FTILMYGLFAAVSLQKSVRDRLEGIPVTGIYFGLCWMSVGLAVLLPTIGLWNADLANS
                     EKGFYAMSFVLSLFAAVAVQKNIRDVAMVREEPQAEWTDPGEDF"
     gene            1017..1742
                     /gene="meaB"
                     /locus_tag="AC161X_03275"
     CDS             1017..1742
                     /gene="meaB"
                     /locus_tag="AC161X_03275"
                     /inference="COORDINATES: protein motif:HMM:TIGR00750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HIR4988599.1"
                     /translation="MSSKELIDRVRSGDRAALARLVTAIESGDPDAIETVASSGEVAG
                     SARRIGVTGPPGAGKSTLISAMIGELRSRGASVAVLAVDPSSPLTGGALLGDRIRMQG
                     HVDDPGVFIRSMATRGHLGGLSKAAEATISALAEAGFDYLVVETVGVGQSEVEVMDLV
                     DVVVLVVGPGWGDQIQAEKAGVVEIADLFVVNKADRPGADEVRRALMSASGESGRQVM
                     ATTATTGQGVDAMVDEIDRLLSR"
     gene            complement(1739..>3857)
                     /gene="ppdK"
                     /locus_tag="AC161X_03280"
     CDS             complement(1739..>3857)
                     /gene="ppdK"
                     /locus_tag="AC161X_03280"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0006090 - pyruvate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HIR4988600.1"
                     /translation="LGADDLEKATRRFQAIVEEQRPGAMPSDPVEQLHGAISAVFSSW
                     NTKRAIEYRRINAIPDDLGTAANVQMMVFGDLGDDSGTGVCFTRDPGTGARLVYGDYL
                     PRAQGEDVVAGIRNPLTLDELGELHPKCHRQLLEIMDGLEAHYRDMCDIEFTIEHDVL
                     YILQTRAGKRTAAAAVMMAVAMVEEGLIDRATAVQRVAPASLEQLHRPRIADHVKQSN
                     AAVTGVAASPGAAIGRAVFSSDDAVAEAKTGEIVILIRPETTPNDIHGMAAAIGILTS
                     QGGKTSHAAVVARGMGKPAVTGAAGLIVDVEAGIARTPAGEIRAGDLVTIDGSTGTVF
                     IGSVDVVAPESPPELHQLLEWADEYRELAVWANADNAKDAAIARKHGAEGIGLARTEH
                     MFLGERLQVVQRIILSDDEQERSVALKDLERVQIGDFEGLLEAMDGLPVVIRLLDPPL
                     HEFLPSRFELEHEMRLDAKAGRPIDDLQSMSEHVARWEEDNPMLGLRGVRLGLMVDHL
                     YRMQARAACHALVTRIQAGGNPMLEIMIPLVADVEELIRMREMIEEEVAAAAKESGLE
                     LKVPVGTMIELPRAALTAGAIAKAADFFSFGTNDLTQMTYGLSRDDAEGLFLGDYLER
                     GILASNPFQTLDRAGVGRLVRVATKEGREANPSLTVGLCGEHGGDPDSIAFVHELGLD
                     YVSCSPPRIEGARLAAAQAALSD"
BASE COUNT          669 a         1156 c         1269 g          763 t
ORIGIN      
        1 agtcaccatc acttcatcca gcgcgcctga tcggatcgaa atcgaccagg agaagctggc
       61 agaaaccctc gagtacatcc gtcccgcggt tcaggccgat ggcggtgact tgatcctgct
      121 cggtaccgag ggtgggaccg tcaacatcca aatggtcggg gcgtgtgggg gttgtccact
      181 gtcgatgatg accctgaagg ccgggatcga gcggatcctg atcgaccggg tcccgggtgt
      241 ggatgaggtc aacgccagcg ccgatgcgcc cgccttagaa gagaacgtcg catcagcgga
      301 gagcggcggg atcacccttc ccggccattt gatggcctct cccgacctta tctgaccgtt
      361 ctttgatcca tccctgatcg gcttatccgt gttggtcgcg tccgaattga cctccaatct
      421 ccgtcagggt gtgatcggtc ggagtagcct catgggcacg ctcgaggagc atctgagccc
      481 ctttggaggg ccgtcgtggg ctgggcatta atgctccatt agacaggagt tcagatgacc
      541 cagaccctgc aaagacctac gactgctttt gtcggcgcct cctggcttgc cctgctcgtt
      601 gggatggccg cgttcctgat tggcctgtcg aatgcgacca tggacctgaa tgagaagggt
      661 tattacttca cgatattgat gtacggcctt tttgcggcgg tgtcgctgca gaagtcggtt
      721 cgtgacaggt tggaggggat ccccgtcact ggcatctatt tcgggctctg ctggatgtcg
      781 gttgggctgg ccgtcctgct tccgacgatc ggtctttgga atgcggacct agcgaacagc
      841 gagaaggggt tctacgcaat gtcatttgtg ttgagcttgt ttgctgccgt tgcagtgcag
      901 aagaacattc gtgacgtggc gatggttcgt gaagagcctc aagcggagtg gaccgacccg
      961 ggagaagact tctaggtttc ggcttaggag cctgagcggt ctttaggctc cttccgatga
     1021 gttcgaaaga gctgattgac cgcgtccgat ccggcgacag ggcagccctc gcacgcctgg
     1081 ttaccgccat cgaatcgggc gatccagatg ccatcgagac cgtcgcctca tcgggcgagg
     1141 tcgcaggatc agcgagaagg atcggggtca ccgggccgcc cggcgcgggc aagtccaccc
     1201 tgatcagcgc catgatcggc gaactgaggt cacgaggagc ttcggtggct gtcctggcgg
     1261 tggatccctc gagcccgctc acgggtggtg ccttgcttgg cgatcggatc aggatgcagg
     1321 gacatgtcga cgatcccggt gtcttcattc gctcgatggc cacgcgaggc catctaggcg
     1381 gattgtcaaa ggctgctgaa gcgaccatca gcgcgctcgc ggaggcgggg ttcgactatc
     1441 tggtggtgga gacggtcggc gtcgggcagt ccgaagtgga agtgatggac ctggtcgacg
     1501 tagtggtctt agtggtcggc cctggctggg gtgaccaaat ccaggccgaa aaggcaggtg
     1561 tcgtagagat cgcagacctc ttcgtggtca ataaggcaga ccggcccggc gcggatgaag
     1621 tgcgcagggc gctgatgagc gcctcggggg agtcgggccg acaggtcatg gccactaccg
     1681 ccaccaccgg gcaaggtgtc gacgcgatgg tcgacgagat cgaccggctc ctgagccgct
     1741 agtcgctcag cgcagcctgc gctgcggcca gtcgggcgcc ttcgatccgg ggcgggctgc
     1801 acgacacgta gtcgaggccg agctcatgga cgaaggcgat cgagtccgga tcgccaccgt
     1861 gctctccaca gagacccacc gtcagcgagg ggttggcctc ccgtccttcc tttgttgcca
     1921 cccgcaccaa tcgcccgacc ccggcgcgat ccaaggtctg aaatgggttg gaggcaagga
     1981 tgccccgttc caggtagtca cccagaaaga gcccttctgc gtcgtcacga ctgagcccgt
     2041 aggtcatctg ggtgaggtcg ttagtgccaa acgagaaaaa gtcggctgcc ttcgcgattg
     2101 cgcccgcggt gagagctgcg cgaggtaact cgatcatggt gccgactgga acctttagct
     2161 cgagccctga ctccttggcc gcggcggcaa cctcctcctc gatcatctcc cgcatccgga
     2221 tcagctcctc gacgtcagcg acgaggggga tcatgatctc gagcatcggg ttgccacctg
     2281 cctggattct ggtcaccagg gcatgacagg cggcacgggc ctgcatccgg tagaggtggt
     2341 cgaccatgag accgaggcgg accccacgca gcccgagcat cgggttgtct tcttcccatc
     2401 gagcaacgtg ctcggacatc gattgcagat catcgatggg acggccggct ttggcatcga
     2461 gccgcatctc atgctccagc tcgaagcggg agggcagaaa ctcgtggagc ggagggtcca
     2521 ggaggcggat taccaccggt agcccatcca tggcctcgag caggccctcg aagtcgccga
     2581 tctgcacccg ttccaaatcc ttgagcgcga cggatcgctc ctgctcgtca tcggaaagga
     2641 tgattctctg gacgacctgg agtctctcgc cgaggaacat gtgctcggtg cgggccaatc
     2701 cgattccctc tgccccgtgc ttgcgggcga tcgccgcgtc cttggcgttg tctgcgttcg
     2761 cccacactgc aagctcccgg tactcatcgg cccattccaa aagctgatgc agttcgggtg
     2821 gagattctgg ggcgaccaca tcgacactgc cgatgaagac ggttccggta gagccatcga
     2881 tcgtcaccag gtcaccggcc cggatctctc cagcaggggt gcgggcgatg ccggcctcga
     2941 cgtcgactat gagacctgcc gcccccgtga ccgcgggttt gcccatgccc ctggcgacga
     3001 ccgcagcgtg tgaggttttc cctccctgac tggtgaggat tccaatcgca gcggccatgc
     3061 cgtgaatgtc gttgggcgtg gtctcggggc ggatcaagat gacgatctcg cccgtcttcg
     3121 cttctgcgac agcgtcatcc gaggagaaga cggctcgccc gatggcggcg ccgggtgagg
     3181 cggcgacccc ggtcacggcg gcattggact gcttgacatg atcggcgatg cggggacgat
     3241 gcagctgctc aagcgaggcg ggggcgaccc gctggaccgc ggtggctcga tcgatgaggc
     3301 cctcctccac catggccacc gccatcatta cggctgcggc cgcggtccgt ttgccggctc
     3361 gggtctggag gatgtagagg acgtcgtgct cgatggtgaa ttcgatgtca cacatgtccc
     3421 ggtagtgggc ttccaggccg tccatgatct ccaacagctg gcggtggcat ttcggatgga
     3481 gctccccaag ttcgtcgagc gtcagtgggt tgcggatccc ggcgacgaca tcctcgccct
     3541 gggcacgcgg caggtagtcg ccgtagacga ggcgggcgcc ggttcccgga tccctggtga
     3601 agcagacgcc ggttcccgag tcgtcaccga ggtcaccgaa gaccatcatt tgcacgttgg
     3661 ccgcggtccc cagatcgtcg ggaatggcgt tgatcctccg gtactcgatg gcgcgcttgg
     3721 tgttccagga gctgaacacc gcggatatgg ctccgtgcaa ttgctcgact gggtcggagg
     3781 gcatggcgcc gggcctctgc tcttcaacga tcgcctggaa gcgacgcgtc gccttctcca
     3841 gatcgtcggc gcccaat
//
LOCUS       DBNHXU010000124         5427 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3412282, whole genome shotgun sequence.
ACCESSION   DBNHXU010000124 DBNHXU010000000
VERSION     DBNHXU010000124.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5427)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5427)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5427
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3412282"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC161X_03285"
     CDS             <1..389
                     /locus_tag="AC161X_03285"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR4988601.1"
                     /translation="IRQVRDEVADLMVRINEYRAGQGLNALTTSAVFTDVAETRAAGM
                     YTTGRISRDTPAGGSVLDDLAAAGVRPTLAGENLALASSSRAAFDGMLDSPTALAQLN
                     EPGYDRAGVAVVEGPTGRLVVVVFGG"
     gene            complement(451..1338)
                     /locus_tag="AC161X_03290"
     CDS             complement(451..1338)
                     /locus_tag="AC161X_03290"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR4988602.1"
                     /translation="MTLLAALAGALAISFSGIFFELSGVDPVTGAFFRLAYALPVLFV
                     IWFVFRHRDQRTMQKRLLAFGSGFLMGVDMIAWHTAIGYIGAGLSTLIANSQVVIVAI
                     AAWLIFRERPSRQILVAIPVVMIGVALVSGLGQENAFGSNPLLGTLLALVSAVFYAAF
                     ILVFRNANDIQAPPAGPLMEATAGGLVTVLLLSPFTGGIDFAPTWPGHAWLIALALGA
                     QVGGWLLIGYALPRIPAVETATIILVQPVLTMIWASMIFDERPSTIQLVGAAMVLAGV
                     GFVAVARARSVPQPVPTTV"
     gene            complement(1373..2797)
                     /locus_tag="AC161X_03295"
     CDS             complement(1373..2797)
                     /locus_tag="AC161X_03295"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0045017 - glycerolipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WS/DGAT/MGAT family O-acyltransferase"
                     /protein_id="HIR4988603.1"
                     /translation="MSDFHYEVLTYLDASFLALETRTSHMHVAGVALFNSAPLKGPEG
                     GIHFDRIKAHILSKLQYIPRYRQRLDWVPYNRHPVWVDDDQFDFEYHVRHTSLPRPGS
                     DVQLKALAGRIVSTPLDRNKPLWELWVVEGVDEDRFAIIAKIHHCMIDGLSGVDLTTI
                     LLNVVPTTEIEAAPQWTPRPAPTPSQLAVAEMAHATRRLIDRLTNLSETVRDGKDLAD
                     RTMDKSAAALSSLRSGWLTTSDRTPLNPDIGPNRRFDWTDIPLADLKQLKNKLGGSLN
                     DVVLTIAAGAIRNFLIEERGYDVSEAAFRVMNPVSTRRADQKGALGNQVAMWLIDLPI
                     EEPDPLARHQLIKERTLNLKKSNQALGAATLVEVSSGTPITLLSLANRVVGSRMRPFN
                     MTVTNIPGPQFPMYLLEAEMLANYPMVPLWAQHGLGIALFSYNGRMLWGIHADYDTFP
                     DSDVFLKAIHASYAELRDLEVPSA"
     gene            2903..3667
                     /locus_tag="AC161X_03300"
     CDS             2903..3667
                     /locus_tag="AC161X_03300"
                     /inference="COORDINATES: protein motif:HMM:NF014609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HIR4988604.1"
                     /translation="MPRQCQILRVFTREEAGGNHLGVVTDLNGLTPETMQAIATDLGF
                     SETIFCDLAGDIPSARIFTPGIEMPFAGHPLVGLGWYLNETLPDPIERIRCQVGEIKV
                     SFEGFQTWIEGAGNQPVESIEDPPLGGDVAVSVLMPIPYMVVQFSSPRQIAELVPPPA
                     SLGDVYVWAWEDPGRVAKVRFFADEAGVPEDPATGSGAFALASYFRSIGQPNGSLVIH
                     QGDEIGHPSTIRLRWDPANSSIGGTVVRDEVRLLDI"
     gene            complement(3668..4171)
                     /locus_tag="AC161X_03305"
     CDS             complement(3668..4171)
                     /locus_tag="AC161X_03305"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018519499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988605.1"
                     /translation="MRSLSPVELKTDRLLLTPLKVEDAAEMVSVLADPDLYGFTGDSP
                     PSLEVLEHRYRAQVIGPPRKGELWHNWILRLAGPETAIGFVQATVIGDSAELAWVVGT
                     AWQGHGYATEGAKAMRDWLAEVGTTQFTAHIHPDHEVSGRVATGIGLRRTDEVDPDGE
                     VIWVSAV"
     gene            4273..>5427
                     /gene="nhaA"
                     /locus_tag="AC161X_03310"
     CDS             4273..>5427
                     /gene="nhaA"
                     /locus_tag="AC161X_03310"
                     /inference="COORDINATES: protein motif:HMM:NF018648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015385 - sodium:proton antiporter
                     activity [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA];
                     GO_process: GO:0006885 - regulation of pH [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaA"
                     /protein_id="HIR4988606.1"
                     /translation="MNREGTNRKLIQPLRDFLATESAGGIVLVVAALIAIVWANSPWQ
                     SSYEQLWTTTFEIRLGSWALSMDLRHWVNEALMAIFFLVVGLEIKRELVEGELRDPSR
                     RALPVYAAVGGMVVPALLFIAFNPAHPEIRGWGIPMATDIALAVGVVTLVGSRLRPAL
                     KLFLLALAIVDDIGAILVIGLVYSGGIDWAWLLAAAAVIGLTLAFQKRVDALAVYLVL
                     GICLWLTFYLAGVHATLAGVIMGLLAPAKPRLTSDLIDQTELSNIGSVEDVLTTQRLA
                     RSSVSVVEWLEHRIHPWTGFLIVPLFALANAGIPLSSAAVAGAVGSPVAWGIFVGLVI
                     GKPLGILLASGIAVRLGAGSLPEGVDWTAIGGAGLVAGMGFTVSIFISELA"
BASE COUNT          985 a         1644 c         1780 g         1018 t
ORIGIN      
        1 agatcagaca ggtccgtgac gaggttgccg acctgatggt ccgaatcaac gagtaccgcg
       61 cgggccaggg tctgaacgca ctcaccacca gcgccgtgtt caccgatgtc gccgagaccc
      121 gagccgccgg tatgtacacg actggtcgca tctcccggga cacgcctgct ggcggcagcg
      181 tcctcgatga tctggctgcg gccggggtga ggccgacctt ggcgggtgag aacctggccc
      241 ttgccagtag ttctcgcgcc gctttcgatg ggatgctcga ctctccaacc gccctcgccc
      301 aactcaacga gcctggctac gaccgggccg gcgtcgccgt tgtcgagggc ccaaccggtc
      361 ggctggtggt ggtggtgttc ggcggctgaa agccgcttcc taatgcaagg gacgccggat
      421 atggcgctat ctgcattcgg aaacgaacgg tcagaccgtg gtcggcaccg gctgagggac
      481 actccgggcc cgggcgactg caacgaagcc gaccccggcc aggaccatgg cagcaccgac
      541 caactgaatc gttgacggcc tctcgtcgaa gatcatcgac gcccagatca tggtcaatac
      601 cggctggacc aggatgatgg ttgcggtctc gacggctggg atacggggga gggcgtaacc
      661 gatgagcaac cagccgccca cctgggcgcc cagcgcgagg gcgatcaacc aggcgtgccc
      721 cggccaggtt ggcgcgaagt cgataccccc ggtgaacggg ctgaggagga ggaccgttac
      781 caatccgccg gcggttgcct ccatgagcgg gccggccggt ggagcctgga tgtcgttcgc
      841 attgcggaag accaatatga atgctgcgta gaagaccgct gagaccaggg ctagcaacgt
      901 cccgagcaac gggttggagc cgaaggcgtt ttcttgcccc agtccgctga ccagggcaac
      961 cccgatcatg acgactggta tcgcgaccag gatctgcctg cttggccgct cacggaagat
     1021 gagccaggcg gcaatggcga cgatgacgac ctgtgagttg gcgatgagtg tgctcagccc
     1081 ggcgccgatg tatccgattg cggtgtgcca cgcgatcatg tcgaccccca tcaggaagcc
     1141 ggagccaaaa gcgagcaggc gcttctgcat cgtccgctgg tcccggtgcc ggaaaacgaa
     1201 ccagatcacg aacaagaccg gcagggcata ggccagccgg aagaacgctc cggtcactgg
     1261 atcgacaccg gaaagctcga agaagatccc cgaaaacgag atggccaggg cgccggccag
     1321 cgctgcgagc agggtcacgc gacgggccgg ccgtcggcca gatcagggaa attcacgccg
     1381 aaggcacttc caggtcgcgc agctcggcgt aggaggcgtg gatcgccttc aggaagacgt
     1441 cggagtcggg gaaggtgtcg tagtcggcat ggattcccca cagcatccgc ccgttgtagg
     1501 agaagagggc gattcccaac ccgtgttggg cccagagagg gaccatcgga tagttggcca
     1561 gcatctcagc ttcgagcagg tacatgggga actggggccc ggggatattg gtgaccgtca
     1621 tgttgaaggg tcgcattctg gagccaacca cacgatttgc cagggaaagc agggtgatcg
     1681 gggtccctga gctgacttcc accaaggtcg ccgctcccag cgcctgattg cttttcttga
     1741 gattcagggt ccgctctttg atcaattgat gacgggccaa ggggtccggc tcctcgatgg
     1801 ggagatcgat cagccacatc gccacctgat tgcccagtgc gcccttctgg tccgcccgcc
     1861 gcgtcgagac cgggttcatc acacggaaag cggcctccga aacgtcgtaa ccacgttcct
     1921 cgatcaggaa attgcggatc gccccggcgg cgatggtgag aaccacgtca ttgagcgatc
     1981 cgcccaactt gttcttgagt tgcttcaggt cggcaagagg aatgtcggtc cagtcgaagc
     2041 ggcggttggg cccgatgtcg ggattgagcg gagtccggtc cgaggtggtg agccatcctg
     2101 agcgaagcga agacagggcg gcggccgact tgtccatcgt ccggtcggcg agatcctttc
     2161 cgtcccggac tgtctcggag aggttggtga gtcggtcgat gagacgccgt gttgcgtgcg
     2221 ccatctcagc gacggcgagc tggctcggcg tgggggccgg gcggggggtc cattgcgggg
     2281 ccgcttctat ctcggtggtt gggaccacgt tgaggagaat cgtggtgaga tcgactccgg
     2341 agagaccgtc gatcatgcag tgatggatct tggcgatgat ggcgaagcgg tcctcgtcca
     2401 ccccttcgac aacccaaagc tcccagagcg gcttgttcct gtcgagtggg gtggagacga
     2461 tgcgcccggc caacgccttg agttggacgt ccgagccggg cctgggcagg gaggtgtggc
     2521 ggacgtggta ttcgaagtcg aactggtcgt catcgaccca gaccggatgc cggttgtagg
     2581 ggacccaatc caaacgctgt cgatagcggg ggatgtactg aagcttggag aggatgtgcg
     2641 ccttgatccg atcgaaatgg atcccgccct ccgggccctt gagcggagcc gaattgaaca
     2701 gggcgacacc ggcgacgtgc atgtggctgg tccgggtctc cagcgccagg aatgaggcgt
     2761 cgagataggt caggacctcg tagtggaaat cgctcatcgg cagtagaacc tacccgaggt
     2821 catcctcata tgaacccggt gattcgaata ccgagcgcga cgcccggttg cagccccagg
     2881 tcgatcgtct agcttgcagt tcatgccccg ccaatgccag atcctgcgtg tgttcacccg
     2941 ggaggaggct ggtggcaacc atctcggtgt tgtcaccgac ctcaacgggt tgacgccaga
     3001 gacgatgcag gccatcgcaa ctgacctcgg gttctcggag accattttct gcgacctcgc
     3061 cggggacatc ccatctgccc gaatcttcac ccccggcata gaaatgcctt tcgccggaca
     3121 ccccttggtc gggctcggtt ggtacctgaa cgagacgctc cctgacccga tcgaaagaat
     3181 ccggtgccag gtcggagaga tcaaagtcag tttcgagggc tttcaaacct ggatcgaagg
     3241 agcggggaac caaccggtgg agagcatcga agacccgccc ctcggaggtg acgtggcggt
     3301 gtcggtcttg atgccgatcc cctacatggt ggttcagttc tcctcaccga ggcagatcgc
     3361 cgagctggtt ccaccccctg cgtcgcttgg agacgtatat gtctgggctt gggaggaccc
     3421 gggccgggtg gccaaggtcc gattcttcgc agacgaggcc ggtgttcctg aagatcccgc
     3481 aaccggaagc ggagcattcg ctctcgcctc gtacttccgg tcaatcggcc aacccaatgg
     3541 aagccttgtg atccatcagg gcgacgagat cgggcatcct tcgacgatcc gcctcagatg
     3601 ggacccagcg aactcgtcga ttggcggcac cgtggtccgc gacgaggtac ggcttctcga
     3661 catctagtca gacggcgctg acccagatca cctcaccgtc agggtcgacc tcgtcggtgc
     3721 gtcgaagacc gatgccagtg gcgactcgac ctgatacttc atggtcggga tggatgtggg
     3781 ccgtgaattg ggttgtgccc acctcggcga gccagtcgcg catcgctttg gccccttcgg
     3841 tcgcgtatcc gtgaccttgc caggcggtgc ccacgaccca tgccagctcg gcggaatcgc
     3901 cgatcacggt ggcttggacg aacccgatgg ccgtctccgg gccggcgagt ctgaggatcc
     3961 agttgtgcca cagctcgccc tttctcggtg gaccgatgac ctgagcccgg tatcgatgtt
     4021 ccaacacttc gagactcgga ggactgtcac cggtgaaccc gtacaggtca ggatcggcga
     4081 ggacgctgac catctcggcg gcatcctcaa cctttagcgg agtgagcaag agtcgatcgg
     4141 tcttgagttc gacgggtgag agggacctca tcgacatcga ctctggcaga cccacgagct
     4201 gcccgaaccc ggcaatggct atcgtctgtc actgacgagc ggtgtgccgg gaagtctggt
     4261 cggcgagggt ccatgaatcg cgaaggcacc aaccgcaagc tgatccagcc actccgcgac
     4321 ttccttgcca cggagtcggc aggaggcatc gtccttgtcg tcgcggcgct catcgccatc
     4381 gtttgggcca actccccatg gcagtccagc tacgagcagc tctggaccac gaccttcgaa
     4441 atccgactcg gttcttgggc gctgtccatg gatctccggc actgggtgaa cgaagcgctg
     4501 atggccatct tctttctggt cgtcggactg gagatcaagc gggagcttgt cgaaggggag
     4561 ctgagggacc ccagccgccg ggccctgccg gtctacgccg cggtaggggg catggtcgtc
     4621 ccagcactgc tcttcattgc cttcaacccg gctcaccctg agatccgcgg ttgggggatc
     4681 ccaatggcaa cggacatcgc gctggccgtc ggggtggtaa cgctggtcgg aagcagactc
     4741 cgacctgcac tgaagctgtt tcttctcgcc ttggcgatcg tggacgacat cggagcgatc
     4801 ctcgtcatcg ggctcgtcta ctccggggga atcgactggg cctggctgtt ggcggcggcc
     4861 gccgtgattg gcctgacgct cgccttccag aagagggtgg atgcacttgc cgtctacttg
     4921 gtcctcggga tctgcctctg gctgacgttt tacctggcgg gagtccacgc cacactcgcc
     4981 ggagtcatca tgggactgct ggccccggcc aagccccgct tgacgtcgga tctgatcgat
     5041 cagaccgagt tgtcgaacat cggatcagtg gaagacgtcc tgacgactca acgtctggcg
     5101 cgctcatcgg tctcggtcgt ggaatggctg gaacaccgaa tacacccgtg gactggcttt
     5161 ctcatcgtcc cgctctttgc gctcgccaat gccggaatcc ccctgtcatc cgccgctgtg
     5221 gctggggcgg tcgggtcccc cgtcgcctgg gggatctttg tcggccttgt gatcgggaaa
     5281 ccgcttggga ttctcttagc ctcggggatt gccgtccggc tgggcgcggg cagcctcccc
     5341 gagggtgtcg actggaccgc gatcggcggc gccggccttg tcgccggcat gggtttcacg
     5401 gtctcgatct tcatctccga attggcg
//
LOCUS       DBNHXU010000125         2131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3610981, whole genome shotgun sequence.
ACCESSION   DBNHXU010000125 DBNHXU010000000
VERSION     DBNHXU010000125.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3610981"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..784
                     /locus_tag="AC161X_03315"
     CDS             <1..784
                     /locus_tag="AC161X_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR4988607.1"
                     /translation="DIPEAYTALGYIAGVTSQIRLGTLVTGITYRNPALLGKMIATID
                     VLSSGRANCGLGIGWNADEHAGYGWDFPEVADRYALLEDTLEMLPLLWGKGSPSFAGR
                     TFSASELVCYPRPLQKHIPITIGGSGERKTLALVARYADACNLFGKPETIVRKVEILG
                     RHCSENGRDPAAVEVTHLTAAMVGRDRRDLAERIERVRGRDQSVEAYSDRNNAGTVDD
                     LVSLFEAYHDAGATHSIVALPDVAEVGSLEAFGDVIARFNQS"
     gene            821..1402
                     /locus_tag="AC161X_03320"
     CDS             821..1402
                     /locus_tag="AC161X_03320"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015145586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HIR4988608.1"
                     /translation="MAVTSTMLALGTEAPDFSLLDTVSGKTVSLDDFADSPALLVMFI
                     CNHCPYVKHVRSELARFGRDYEDTGLAIVAINSNDPDSHPEDSPEAMKAEAETQGYAF
                     PYLFDEQQTAAAAFTAMCTPDFFLFDRDRRLVYRGQFDSARPANEIPVTGGDLRAAAQ
                     AVLSGEQVSEIQRPSMGCSIKWRSGNTPAYFVS"
     gene            1443..1952
                     /locus_tag="AC161X_03325"
     CDS             1443..1952
                     /locus_tag="AC161X_03325"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR4988609.1"
                     /translation="MANQYPGPPDLLIDLDKTYAATVDTNHGVIEIEFDAVRSPIAVN
                     NFVFLSREGYYDGIVFHRVIENFMIQGGDPTGTGSGGPGYKFRDELEGEGNYARGTVA
                     MANAGPNTNGSQFFICHRDAGLPHSYTIFGKVTSGMEAVDSIAGTETDRRDRPTEDAI
                     INSITVTEI"
BASE COUNT          430 a          674 c          632 g          395 t
ORIGIN      
        1 ggacatcccg gaggcctaca ccgcgcttgg atacatcgcc ggcgttacca gccaaattcg
       61 gcttgggacg ctggtgacag gcatcaccta ccgcaacccg gcgctgctcg gaaagatgat
      121 cgccaccatc gatgtccttt cgtccggccg ggcaaactgc ggattgggca tcggctggaa
      181 tgccgatgag cacgccggtt acggatggga cttccccgag gtggctgatc gctatgccct
      241 gctcgaggac acgctcgaga tgctccctct cctctggggc aagggctcgc cttccttcgc
      301 agggcgcacc ttctcggcgt cggagctggt ctgctacccc cgaccgctcc agaagcacat
      361 cccaatcacg atcggtggct caggagagcg aaagacgctg gccttggtcg cccgatacgc
      421 cgatgcatgc aatctgttcg ggaagcctga aaccattgtc cgcaaggtgg agatcctggg
      481 ccgccattgc tctgaaaatg gacgggaccc cgccgcggtc gaagtcaccc acctcacagc
      541 agcgatggtg ggccgtgacc gtcgagacct tgcggaaagg attgagcggg tccgtggtcg
      601 tgaccaatcg gttgaggctt actcggacag gaacaacgcc gggactgtgg atgacctggt
      661 gagcctgttc gaggcgtatc acgacgcagg tgccactcat tcgatcgtcg cccttcccga
      721 cgttgcggag gtgggcagcc tcgaggcatt cggcgatgtg atcgcacggt tcaaccagtc
      781 ttagacttcg agaaccgatc tcacggaagg accttcatcg atggctgtta cgtcgaccat
      841 gctcgccctc ggcaccgagg cgccggactt ctcgcttctg gacaccgtgt ccggcaagac
      901 cgtgtcgtta gacgacttcg ctgattctcc ggccctgctg gtgatgttca tctgcaatca
      961 ctgcccttat gtcaaacatg tgcgctcaga attggcgcgc ttcggccgcg actacgagga
     1021 caccgggtta gcgatcgtcg ccatcaactc gaacgaccct gattcgcatc ccgaagactc
     1081 ccctgaggcg atgaaagccg aagccgagac ccagggctat gccttcccgt acttgttcga
     1141 cgagcagcag acagcggcgg cggccttcac cgcaatgtgc acgccggact tcttcctctt
     1201 cgaccgggac cggcgcctcg tctacagggg gcagttcgac agtgcccggc cagccaacga
     1261 gatcccggtc accggaggcg accttcgtgc cgcagcgcag gcagtgctgt cgggcgagca
     1321 ggtgagtgag attcaaaggc cgtcgatggg gtgctcgatc aagtggcgga gcggcaacac
     1381 ccctgcctac ttcgtcagct gaccagccgc atccgaatcc ccgaccagaa agcagagcaa
     1441 ccatggccaa ccaatacccc ggcccacctg acctcttgat cgacctcgac aagacctatg
     1501 cggccacggt cgacaccaac cacggtgtca tcgagatcga gttcgacgcg gtgcgctcgc
     1561 ccatcgcagt caacaacttt gtcttcctca gtcgggaggg ttactacgac gggatcgtct
     1621 tccatcgcgt gatcgagaac ttcatgatcc agggcggtga cccgactgga accggcagtg
     1681 gcgggcctgg ctacaagttc cgcgacgagc tcgagggtga gggcaattac gcacggggca
     1741 cggtggcgat ggccaacgcc gggccgaata cgaacggctc ccaattcttc atatgccatc
     1801 gggatgccgg gctccctcat tcctacacga tcttcggaaa ggtgacctcg ggaatggagg
     1861 cagtcgactc gatcgcaggc accgaaaccg accgtcgcga ccgcccgaca gaggacgcaa
     1921 ttatcaactc gatcaccgtg acagaaatct gattgctctg ttatcctccc ggcgctgttg
     1981 ctgcctcggc ggcgactcgt tctgacttcc ccgttcgaat gactgggctg aggacaaggg
     2041 tcgtaccgga cgttgcgacg taaagcccac aagggccgta gagacaagga ccagtcacaa
     2101 tgaacatcta cgtgggaaac ctgccgttca a
//
LOCUS       DBNHXU010000126         1837 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1626659, whole genome shotgun sequence.
ACCESSION   DBNHXU010000126 DBNHXU010000000
VERSION     DBNHXU010000126.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1837)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1837
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1626659"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..611)
                     /locus_tag="AC161X_03330"
     CDS             complement(<1..611)
                     /locus_tag="AC161X_03330"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012891212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR4988610.1"
                     /translation="MRALVLHGPGVARVDDVDPPTPTTGLVVVDVERVGICGTDVEFF
                     TGEMDYLRQGHTSYPLRIGHEWAGRVTALGDGVDLTWLGKRVTGDTMLGCGTCDRCLS
                     GRHHVCESRQEIGVRGGWPGALAEQLAVPESALYEVPEQVSSAAAALVEPGGCAVRAL
                     NGTALQAGQRLLIFGPGTIGLLVAQFAIARGIEVHMVGVESAGL"
     gene            670..>1837
                     /locus_tag="AC161X_03335"
     CDS             670..>1837
                     /locus_tag="AC161X_03335"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="esterase/lipase family protein"
                     /protein_id="HIR4988611.1"
                     /translation="MVQRMTADEVRGLSGLVDLVVERVTAPVEGMHRAIADRSLSWTG
                     PTGQPARRVFDGTIAALYDVIRLGGSVVGAAIGLAAGARADSSIETPSKRKVQAAVNA
                     TWGDELVQRDNALRIDMGIRQPGGALVELTPVSVTADFPSATSRIVVLVHGLGRTEDC
                     WLEKDDRAGLWDRLATDSSVTPVMVRYNSGRHVSDNGADLAEILEQLWRCWPVSIETV
                     TLVGHSMGGLVIRSACQVGKAAGNEWIDGLDHVITVATPHLGAPLEKAVNIVSWGLRA
                     TPESSPLAEFLDARSAGIKDLRFGRIVAGDWSETEQSTLFQNSPADPPPLPDVQHHFV
                     AAVVTRDPRHPVGAMFGDLMVRAASGTGRGRLRKIEATDTQVLGGRRHFDLLHDS"
BASE COUNT          339 a          601 c          569 g          328 t
ORIGIN      
        1 aggcccgcac tctcgacgcc aaccatgtgg acttctatgc ctcgggcgat cgcgaattgg
       61 gccacaagaa gcccgatcgt ccccgggccg aagatcagta ggcgttgtcc cgcctggagt
      121 gcagtcccgt tcaatgcacg gacggcgcaa cccccaggct caactagcgc cgcggccgcc
      181 gacgaaacct gctctgggac ctcgtagagg gcagactcag ggacggccaa ttgctcagcc
      241 aacgcacccg gccagcctcc gcgaaccccg atttcttgac gggattcgca aacatgatga
      301 cgcccgctca gacatcggtc gcacgtccca caaccaagca tcgtgtcgcc ggtgacacgc
      361 tttcccaacc aggtcaggtc cacgccgtcg cccagcgcag tgacccggcc cgcccattcg
      421 tgacctattc gaagcggata tgaggtgtgg ccctggcgga gatagtccat ttcccctgtg
      481 aagaactcga cgtcggtgcc acagatccca acccgctcga cgtcgactac caccaagccg
      541 gtcgttggag tgggggggtc cacatcgtcg actcgggcca ccccggggcc atggagaact
      601 agtgctcgca tccccccaag tattccgtcc ccttcggcct tgtgtcgatc tgtcgttacc
      661 gtcttcatcg tggttcagcg aatgacggcc gatgaggtcc ggggtttgag cggcctggtc
      721 gacctcgtgg tcgaacgggt caccgcgccc gtggaaggga tgcaccgagc tatcgcagat
      781 cgctcactga gctggacagg ccccaccgga caacctgctc gacgcgtgtt cgacggcacc
      841 atcgctgctt tgtatgacgt catccgcctg ggcggctcag tcgttggagc cgcgatcggg
      901 ctcgcggcgg gtgcccgggc tgacagctct attgagacac cgtccaaacg caaggttcag
      961 gcggctgtga acgccacatg gggcgacgag ctcgtccagc gagacaacgc ccttcgcatc
     1021 gacatgggca tccggcagcc tggaggcgcc cttgtcgagc taaccccggt cagcgtgacg
     1081 gcagactttc catcggcgac ctcccggatc gtggtcctgg tccacggatt gggtcgaaca
     1141 gaggattgct ggctggagaa ggatgaccgc gccggtctgt gggatcggct tgcgaccgac
     1201 tcgtctgtca ccccggtgat ggtccgttac aactcgggtc gccacgtgtc ggacaacggt
     1261 gccgatttgg ccgaaatcct cgaacagttg tggcgctgct ggccggtctc gatcgagacg
     1321 gtgacgctcg tcggccactc gatgggcggt ttggtgatcc gaagcgcctg ccaagtcgga
     1381 aaggcggcag gcaacgaatg gatcgacggg ctggatcacg tgatcaccgt cgctacgcca
     1441 catctcggcg cgccgttgga aaaggccgtc aacattgtct cgtggggcct cagggcaact
     1501 cccgaaagca gccctttggc tgaatttctc gacgctcgca gcgcgggcat caaggacctt
     1561 cgatttgggc gcatcgtcgc aggagactgg tccgagaccg aacagagcac cctcttccag
     1621 aacagcccag ccgaccctcc gccgttgccc gatgtgcaac accactttgt ggcagctgtt
     1681 gtcaccaggg acccccgcca tccggtcgga gcgatgttcg gagacttgat ggttcgggca
     1741 gccagcggaa ccgggcgggg tcgactacgc aagatcgaag ctaccgacac ccaggttctg
     1801 ggcgggcgga ggcacttcga cctgctccac gactcag
//
LOCUS       DBNHXU010000127        10010 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2618524, whole genome shotgun sequence.
ACCESSION   DBNHXU010000127 DBNHXU010000000
VERSION     DBNHXU010000127.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 10010)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 10010)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10010
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2618524"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..600)
                     /gene="rpsP"
                     /locus_tag="AC161X_03340"
     CDS             complement(130..600)
                     /gene="rpsP"
                     /locus_tag="AC161X_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003781530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HIR4988612.1"
                     /translation="MAVKMRLIRMGKKKSPVYRVVVVDGRSPRDGRYIEQIGRYDPRQ
                     EPSLVEIDNDKATEWLAKGAQPTEAVEKLLNISGALTTKKVKQGQIHVVPAAAQSQEE
                     EPESARVAPTHEEAAAAKSAAVEEPAPIVEEVAEAPEAEAADEVEAAAISSEEE"
     gene            complement(605..1939)
                     /gene="ffh"
                     /locus_tag="AC161X_03345"
     CDS             complement(605..1939)
                     /gene="ffh"
                     /locus_tag="AC161X_03345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013175088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HIR4988613.1"
                     /translation="MFDTLTRRFDGVFSSLRSKGRLSEKDIDDALREVRLALLEADVN
                     VGVVKAFLGRVKERALGEDVGRSLTPGQQVIKIVHEELVITLGAESTGLVKAASPLTI
                     LMVGLQGSGKTTTAAKLAAHLRSQGKKPLLVAADLQRPAAIDQLETLGARIQVPVFAD
                     RKGKPEKLVKAALKEAVRTGRDVVIVDTAGRLQIDPQLMKELADVRKAAEPDEVLLVV
                     DAMTGQDAVNVANGFQQQIGLTGVILSKLDGDARGGAAISVREVTGAPVKFAGVGEGL
                     EDLEPFYPDRMASRILGMGDMLTLIEKAESTFDQAEAEKTAKKLEKGQFTLEDFLAQF
                     QQMKRMGPLKDILAMLPGSGSLLRDIDVDDRDLRRVEAIIQSMTPEERRNPKVIGGSR
                     KRRIASGSGSRPQDVNRLLKQFADAQKMMKTLAGGKGAPSLQGLLSAQRTKR"
     gene            2001..2348
                     /locus_tag="AC161X_03350"
     CDS             2001..2348
                     /locus_tag="AC161X_03350"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4988614.1"
                     /translation="MSFNIDTVFVWVSDIDRSVPWYRDLGVEPGPRHGTWQAMATEGG
                     ARFGLHQGERQRGEPTAAVAFGVSDLDAEMQRLAALGIDPADAEVTDTGAARFITYRD
                     PDGNEVQLLERRG"
     gene            2341..2805
                     /locus_tag="AC161X_03355"
     CDS             2341..2805
                     /locus_tag="AC161X_03355"
                     /inference="COORDINATES: protein motif:HMM:NF021386.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HIR4988615.1"
                     /translation="MAEPPITAEEFETRLLAICTGGGGPGLPRRQRDVAVIMASATLW
                     MAPGSLYTEAEINEGLEGWVTEICPSLGLDPVTLRRELVDRNYLTRDDSGTSYAPGPG
                     PVEWRFEDGVADIDSKALVTSAVEVREARKRAYLARSQSGAGDGLSSDSEQF"
     gene            3247..4122
                     /locus_tag="AC161X_03360"
     CDS             3247..4122
                     /locus_tag="AC161X_03360"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR4988616.1"
                     /translation="MYPSRFRYEAPRSIEEASSLLSDGGGSAKILAGGQSLVPMIKLR
                     FASPEMLVDINGLPGLGYHNLDADGSIHIGALCRHADLERSSLLASHQPTMAAAAPLV
                     ADPIVRVRGTLVGSLCHADPQGDWASVMTSLDGSVVAEGPNGRRAIPVRDFVRGPFQT
                     VLAVDEIVVEAVVPAPKGVASGGYLKLERRVGDFATAGVAVSIDREGSKVTRAGIALT
                     GVGGSTISADEAAQLLVGGSLSADLIAEAAELTAQAAQPRSDHRGSAEFKRQVVRVFT
                     TRLLTRLLVSEEEAA"
     gene            4124..4645
                     /locus_tag="AC161X_03365"
     CDS             4124..4645
                     /locus_tag="AC161X_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013674780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR4988617.1"
                     /translation="MNNMYEVLPTESDEDVPVRKVTIRVNGENRSAVVEDRLLLAHLL
                     RQGFQLTGTHTGCDTTNCGACTVLFDGKPVKSCTMFAVQADGHDVMTVEGLAGASELH
                     AIQEGFKEEHGLQCGFCTPGMMMSTKALLDENPNPTEDEIRWALSGNLCRCTGYQNIV
                     KAVMWAATKLQDQ"
     gene            4703..7051
                     /locus_tag="AC161X_03370"
     CDS             4703..7051
                     /locus_tag="AC161X_03370"
                     /EC_number="1.2.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0043885 - anaerobic carbon-monoxide
                     dehydrogenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aerobic carbon-monoxide dehydrogenase large
                     subunit"
                     /protein_id="HIR4988618.1"
                     /translation="MGASRRRVEDNRFIRGKGNYVDDIVLPGMLHMEILRSPLAHARI
                     RSIDASKAWAMPGVRLVLTGETMAARNLAWMPTLSYDTQAVLATDKVRFQGQEVACVI
                     ADDPYIAKDACEAIVVDYEPLPPIVNPMQSLAAGAPLIRDDKENQADNVIYDWEVGDK
                     NGTDRAFAEADLVSKIELHYPRSHPSPIECCGSIADYNRATSKLTVYMTSQAPHIIRA
                     AVAMVAELPEQMIRIIAPDIGGGFGNKVPVYPGYVASILASILLERPVKWIEDRTGNL
                     ISTGFARDVYLHGELALRKDGRILGVRMETTADHGAFFSDAQPSKFKIGLMHSAFACY
                     DIPNAHLVARGVYTNKAPGGVAYRCSFRVTEAMFFQERMVQAAADDLGMDQAEFRRIN
                     FVRDDDFPHRTAYGFLTDSGQYGKCLDVGLEAIGYQDFLKQKEEAAKRGRLLGIGIST
                     MTEPLGAGNSREYDILGIKMFDSAELKVHMTGKALLRTGAKSQGQGHETTWAQIVAHE
                     LGIPAEDITVEEGDTDTAPFGMGTYASRSTPVAGAAVAMVSRKIRAKARKIAAHLLEI
                     SEEDVEWELGRFYVRSQPDRGVTIQECAVAAYSNMPDGMEPGLDNTAYYDPPNLTWPF
                     ACYIVTVEVDPETGVWDVLRLVAVDDCGVRINPMIVEGQIMGGLTEAYAMASMQFITF
                     DAEGNCIGSNFTDYLLPTAWETPGFELHEVVTPCPHHPIGAKGIGECAAVGGPAAFVN
                     AVIDALKGTGVRNIDMPVFPDRVYEALQTGKDVSGIPPVRME"
     gene            7053..8042
                     /locus_tag="AC161X_03375"
     CDS             7053..8042
                     /locus_tag="AC161X_03375"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR4988619.1"
                     /translation="MNSQPVDGWRMVEKASELARQGVEFALATVVWRQAPSSGHQGAR
                     AIITADGQLHGWIGGACAEPVVIREAQRVIEEREPRLILLGTPDQFGELPEGMMFVQM
                     SCQSEGALELYIEPVLASPHLVVVGHSPMATTLVDLATVLGWRADLFEAAEFTTADVN
                     SASVVVIATQGHGDEEAVEQAVAGKPAFVGLVASRKRGEAVLGYLADRGVSKELLDEV
                     RVPVGFDLGHTSHPEIAVAVLAELVELRAKGALSKRAADTKVAIPVAEAMTPGTAIDL
                     VCGMTVPADNTSRPFEFEGVTYYFCCPGCRVAFEKDPAKFIKKEELHADQARL"
     gene            8020..8604
                     /locus_tag="AC161X_03380"
     CDS             8020..8604
                     /locus_tag="AC161X_03380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4988620.1"
                     /translation="MLIKHDFEVPLPVDRVWEFCQDIPQVASCLPGASLTDQVGQDQY
                     HGNVAIHLGPVQMQFAGRADVTKRDEASKTIVIDASGADAKGRGQAALGLTARLTPTA
                     TGTKVSVEQDLQLSGAAAQYGRGMVSDFSAVMLRDFATNMQVRIDAFDRGLSPDQIQQ
                     AKSAGGFSLAIQAAWMALKRVGRRFFLPYQPNQI"
     gene            8615..8866
                     /locus_tag="AC161X_03385"
     CDS             8615..8866
                     /locus_tag="AC161X_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988621.1"
                     /translation="MLWWIGNAVLLLVVVPVLVALLNRVLAACERIRGASDQILGGGV
                     GLIGELDRVPGLLGVTDKAIEDVSLGAVRYAGSVGKLLG"
     gene            8882..9109
                     /locus_tag="AC161X_03390"
     CDS             8882..9109
                     /locus_tag="AC161X_03390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988622.1"
                     /translation="MPATAIVTLVIATLIIAAAALGLMRVIGHLSATLKTLRALTGGV
                     GVVAQQTSGVPAAVASINANLKPVSDFCESI"
     gene            9121..9381
                     /locus_tag="AC161X_03395"
     CDS             9121..9381
                     /locus_tag="AC161X_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988623.1"
                     /translation="MNELFEGNTGWLVGYIIGIVVVLVVVALVVPILVLARRIGNEAQ
                     LIDDSLKESVVNTAALADLATTNTMAVNVIEGLKRGRLRLGG"
     gene            9384..9671
                     /locus_tag="AC161X_03400"
     CDS             9384..9671
                     /locus_tag="AC161X_03400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988624.1"
                     /translation="MIFALTEDQETLWQIANWAGLVVVVAVAALLTILVLLVRTIERR
                     VAGIKATLTEAEANTSDAALLNTTAAGVDLVLAEGLEHHLFLGRVLGKVRS"
     gene            9668..>10010
                     /locus_tag="AC161X_03405"
     CDS             9668..>10010
                     /locus_tag="AC161X_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011753732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988625.1"
                     /translation="MNSLIVFTVVDIVLLIAGLAIYLWIVGGQLTRVATGLEECNDLV
                     QKIKDNAASIEGNLEHINRTGGVIAGALPLLYGMAEGIVVGATYDPEKVIERGPAKPA
                     SGTRRSRMHEGV"
BASE COUNT         1828 a         3229 c         3135 g         1818 t
ORIGIN      
        1 ttctcacttc ggcgatcagc tcgtcaggcc cctgctccac catggtgacc gtgacttcgt
       61 ccggctcttc aacaatgctc ttcaccacgt aggtgacgac tctttcgacg atttcaccct
      121 tggccacagt cattcctcct cggaggagat ggcggcggcc tcgacctcgt cggcggcctc
      181 agcttcgggg gcctcggcga cttcttccac gatcggtgcc ggctcctcaa ccgcggctga
      241 cttcgccgca gcggcctctt cgtgggtcgg cgccacccga gccgactcgg gctcctcctc
      301 ctggctctgt gccgcagcag gcaccacgtg tatctggcct tgcttcacct tcttggtggt
      361 caaggcgcca gagatattga gcagcttctc aactgcctcc gtgggctgag ctcccttggc
      421 cagccactcg gtcgccttgt cgttgtcgat ctcgaccaac gacggctcct ggcgcgggtc
      481 ataacgccca atttgctcta tgtaccggcc gtcacggggg gaacgcccgt ccacgaccac
      541 gacccggtag acgggcgact tctttttgcc catccggatc agtctcatct tcacggccat
      601 cgactcacct cttggttcgc tgcgccgaca ggagtccctg aagggaaggg gctcccttac
      661 ctccggccag cgtcttcatc atcttttgtg catcggcgaa ttgctttagg aggcgattca
      721 catcctgggg cctgctgccc gaaccggacg cgattctccg cttgcggctc ccgccgatga
      781 ctttcgggtt ccggcgctcc tcaggggtca ttgactggat gattgcctcg acccttctca
      841 gatcacggtc atcgacatcg atgtcgcgaa ggagcgatcc ggagccggga agcatagcaa
      901 ggatgtcttt cagaggcccc atccgcttca tctgctgaaa ctgggccagg aagtcctcga
      961 gggtgaactg gcccttctcc aacttcttcg ccgtcttctc ggcttccgcc tggtcgaatg
     1021 tcgattccgc cttctcgatc agggtcaaca tgtcgcccat ccccaggatc cgcgatgcca
     1081 tccggtccgg ataaaacggc tccagatctt ccaggccctc cccaactccg gcgaatttca
     1141 ccggggcccc cgtcacttcc cgaaccgaga tcgccgcccc acccctggcg tcgccatcga
     1201 gcttggagag aatgacgccg gtgagcccga tttgctgctg aaagccattc gcgacgttga
     1261 ccgcgtcctg gccggtcatc gcgtcgacga cgagaaggac ctcgtcgggc tcggccgctt
     1321 tgcgaacgtc ggccaactct ttcatgagtt gtgggtcgat ttggagccgg cccgcggtgt
     1381 cgacgatgac gacgtctcgg ccggtgcgca ccgcctcctt gagggctgct ttgaccagct
     1441 tctccggctt tcccttgcgg tcagcgaaca ccggtacctg aatgcgggcc ccgagagtct
     1501 cgagctgatc gatcgcggcc gggcgctgta ggtcggctgc caccagcaag ggcttcttcc
     1561 cctgagagcg gaggtgggcg gcaagcttgg ccgcggtcgt cgtcttgcca gatccctgga
     1621 gcccgaccat caggatggtg aggggcgaag cggccttgac cagaccggtc gattcggcgc
     1681 cgagtgttat caccaactct tcgtggacga tcttgatcac ctgttggcca ggggtgagcg
     1741 accggcctac gtcttcgccg agggcgcgct ctttcacccg accgagaaag gctttgacga
     1801 caccgacgtt tacgtccgcc tcgagcaggg caagccgcac ctcgcggagg gcgtcatcga
     1861 tgtccttctc cgagagccgc cccttgctcc tcagcgagct gaagactccg tcaaaacgcc
     1921 tggtgagtgt gtcgaacacg acggtcacgc taccggggtc ggggtcactg tgagcactcc
     1981 gctagagata gtctcgggtc atgtccttca atatcgacac cgtgtttgtc tgggtcagcg
     2041 acatcgaccg atccgtgccc tggtaccggg acctaggggt cgaacccggc ccccggcatg
     2101 gcacctggca ggcgatggcg accgaaggtg gagctcgttt cgggcttcat cagggggaac
     2161 gccaacgtgg tgagccgacg gctgcagtcg cgttcggcgt ttccgacctc gacgctgaaa
     2221 tgcaacgact ggccgccctg ggcatcgacc cggcggatgc cgaggtgacc gacaccggcg
     2281 ccgccagatt catcacctac cgcgacccgg atggcaacga ggtgcagctc ctggaacgcc
     2341 gtggctgagc ctccgatcac ggccgaggaa ttcgagaccc ggctgctggc gatttgcacc
     2401 ggaggaggtg gccctggact gccccgacga caacgtgacg tcgccgtgat catggccagc
     2461 gcaacactat ggatggcacc gggctcgctc tacaccgagg cagagatcaa cgagggcctg
     2521 gaggggtggg ttacggagat ctgcccgtcc ctcggcttgg acccggtgac ccttcgccgc
     2581 gagctggtcg accgcaacta cctgacccgt gacgactccg gcaccagcta cgcgccagga
     2641 cccgggccag tggagtggcg cttcgaggac ggagtcgccg acatcgactc caaggccttg
     2701 gtgaccagtg cggttgaagt gagggaggcc cgaaagcgtg cctaccttgc ccgaagccag
     2761 tctggagcag gcgatggact gtccagtgat agtgaacagt tttgacacca cgagccagaa
     2821 ctactgcgta tgctgaatcg gcgacgggca cacgtttcgc gccgaccgct tgtacgaaag
     2881 aggttgagat tccaccgagc aacagggcct ccggtcatac cgatcggccc cgaggactgg
     2941 aggtttgctt tggcagttga gacgtctggc cacagtcgcg gtaacccacg gttgtatgag
     3001 ctgatcgaaa acgccccgaa gaccggcaag agcccgacta tctgggcctg gcctgagata
     3061 gtcgactacc tcaagacggc cggccagccc gtgcagggac cgtggccgcc ccaccagcag
     3121 ccgagaccgg atcccgctgc cgagtctctc ccggttgccg tgcccgaccc acagtctttt
     3181 ccttctcacc gcgaccacga tccggattcc ggcccgcgga cctgaacagg accaaggagg
     3241 caagccatct atccctctcg atttcgttac gaggccccac gctcgataga ggaggcttct
     3301 tccctcctct ccgacggagg cggaagcgcc aagatcctcg ccggtggcca gagcctggtc
     3361 ccgatgatca agctgcgatt tgcctccccg gagatgctcg tcgacatcaa cggccttcct
     3421 gggcttggct atcacaatct ggatgccgat gggagcatcc acatcggggc actgtgcaga
     3481 catgccgacc tcgagcggtc ctcgctgctg gcctcccacc agccgacgat ggcggcggcc
     3541 gctccccttg tcgccgaccc gatcgtcagg gttaggggca ccctcgttgg atcgctgtgt
     3601 cacgctgacc ctcaaggcga ttgggcgtcg gtgatgacct ccctcgatgg gtcggtcgtc
     3661 gccgagggcc cgaacggacg gcgggcaatc ccggtcagag actttgtccg aggcccattc
     3721 cagactgtcc tcgccgtcga cgagatcgtt gtcgaagccg tcgtgccggc gccgaagggc
     3781 gtcgccagcg gcggctacct caaactcgag cgcagggtcg gcgacttcgc caccgccggg
     3841 gtggcggtct caatcgaccg ggagggaagc aaggtgaccc gggcaggcat cgctctcacc
     3901 ggcgtgggtg ggtcgaccat ctcggccgac gaagccgccc aattgctggt cggcggttca
     3961 ctcagcgcag acctgatcgc ggaggcggcc gagctcactg cccaggcggc ccaaccccgc
     4021 tccgatcatc ggggcagtgc cgaattcaaa cggcaagtgg tgcgtgtgtt caccacccgg
     4081 ctcctcacca gacttcttgt gtcggaagag gaagcagcct gatatgaaca acatgtacga
     4141 ggtccttccc accgaatccg atgaagatgt cccggttcgg aaagtcacca tccgggtcaa
     4201 tggcgagaac cgctcggcgg tggtcgagga ccgacttctg cttgcccatt tgctccgtca
     4261 aggtttccag ctcactggca ctcataccgg ctgtgacacc accaactgcg gtgcctgcac
     4321 cgttctgttc gacggcaaac cggtcaagag ctgcaccatg ttcgccgtcc aggctgacgg
     4381 ccacgacgtg atgaccgtcg aaggcctcgc cggtgcgagc gagctccacg ccatccagga
     4441 gggattcaaa gaggagcacg gccttcagtg tggcttctgc accccgggga tgatgatgtc
     4501 caccaaggca ttgctcgatg agaaccccaa ccccacggag gacgagatcc gctgggcgtt
     4561 gagcggcaat ctctgtcgat gcaccggata tcagaacatc gtcaaggcgg tcatgtgggc
     4621 cgcaaccaag ctccaggatc aatgacatgc tttcagcgga ggtgaacagg tgacggcggt
     4681 cgacgaagtc aagatcggcg ggatgggcgc cagtcggcgc cgggtcgagg acaaccgctt
     4741 catccgaggc aagggaaatt acgtcgatga catcgtgttg cccggaatgc tgcacatgga
     4801 gatcctccgc agcccccttg cccatgctcg gatcaggtcg atcgacgcct cgaaagcgtg
     4861 ggccatgcct ggagtgcggt tggttctgac cggcgagacg atggccgccc gcaacctggc
     4921 atggatgccg accctttcct acgacaccca ggcggtgctg gcgaccgaca aagtccgctt
     4981 ccagggacag gaggtggcct gcgtcatcgc cgacgacccc tatatcgcca aagatgcctg
     5041 cgaggcgatc gtcgtcgact acgagccgct ccccccaatc gtcaatccaa tgcagtcgct
     5101 cgccgctggg gcccccctaa tccgagacga caaggagaac caggccgaca acgtcatcta
     5161 tgactgggag gtgggcgaca agaacggcac cgaccgcgca ttcgccgaag ccgacctcgt
     5221 ctccaagatc gagttgcact acccccgctc tcatccctcg ccgatcgaat gctgcggatc
     5281 gatcgccgac tacaaccgtg ccacctcgaa gctcaccgtc tacatgacat ctcaggcgcc
     5341 gcacatcatt cgcgctgcgg tggcgatggt cgccgagttg cccgagcaga tgatcaggat
     5401 catcgccccg gacatcggtg gcggtttcgg caacaaggtg ccggtctatc ccggatatgt
     5461 ggcctcgatc ctggcctcga tcctcctcga gaggccggtg aagtggatcg aggaccgcac
     5521 cgggaatctg atctccaccg gtttcgcccg cgacgtctac ctccatggcg agttggcgct
     5581 gcgaaaggac gggcggatcc tcggggtgcg gatggagacc accgccgacc atggcgcttt
     5641 cttctccgat gcccagccgt cgaagttcaa gatcgggctg atgcactcgg ccttcgcctg
     5701 ttacgacatc ccaaatgccc acctcgtggc ccgtggcgtc tacaccaaca aggctccagg
     5761 cggggttgcc taccggtgct cgttccgggt gaccgaggcc atgttcttcc aggagcggat
     5821 ggttcaggcg gcagcggatg acttgggaat ggaccaggcg gagttccgcc ggatcaactt
     5881 cgtccgcgac gacgacttcc cgcatcgcac cgcgtacggg tttctcaccg actcaggcca
     5941 gtacggcaag tgcctagacg tcgggttgga agccatcggc taccaggact tcctcaaaca
     6001 gaaggaggaa gcggccaaac gcggccggct gctcgggatc ggcatctcca ccatgaccga
     6061 gccactggga gcaggcaaca gccgggagta cgacattctc gggatcaaga tgttcgactc
     6121 ggctgagttg aaagtccaca tgactggcaa ggcgcttctc cgcaccggag caaagagcca
     6181 gggccaggga cacgagacga cctgggctca gatcgtcgcc cacgagctcg gaatccctgc
     6241 cgaggacatc accgtcgaag agggcgacac tgacaccgcc cccttcggga tgggcaccta
     6301 cgcctcgcga agcacaccgg tggcgggagc tgccgtggca atggtttcac gcaagatccg
     6361 ggccaaggcc cgcaagatcg ccgctcacct gctcgagatc tccgaagagg acgtcgagtg
     6421 ggagctcggg cgcttctacg tgcgcagcca gcccgaccgt ggcgtcacca tccaggagtg
     6481 tgcggtcgcc gcctactcca acatgccaga cggcatggag ccgggtctgg acaacacggc
     6541 gtactacgac cctcccaacc tgacctggcc gttcgcctgc tacatcgtga ccgtcgaggt
     6601 cgatcccgag accggggtgt gggacgtgct tcggctggtc gctgtcgatg actgtggggt
     6661 tcggatcaac ccgatgatcg tcgagggcca gatcatgggc ggtctcaccg aggcctacgc
     6721 catggcgagc atgcaattca tcactttcga cgccgagggc aactgcattg gctcgaactt
     6781 caccgactac ctgctgccaa cggcctggga gacccccggg ttcgagctac atgaggtcgt
     6841 gaccccgtgc ccccaccacc cgatcggggc gaagggcatt ggggagtgcg ccgcggtggg
     6901 cgggccggca gcgttcgtca acgcagtgat cgacgccctc aagggaaccg gggtccgcaa
     6961 catcgacatg ccggtgttcc ccgaccgcgt ctacgaagcg ttgcaaaccg gcaaggatgt
     7021 cagtggtatt ccaccggtga ggatggagtg aattgaattc gcaaccggtc gacgggtgga
     7081 ggatggtcga aaaggcgagc gagctcgccc gtcagggcgt ggagttcgcc ctggccaccg
     7141 tcgtctggcg tcaggcgccg tcttctggtc atcagggagc gcgggccatc atcaccgccg
     7201 acggccagct tcacgggtgg atcggtggcg cctgtgcgga gccggtcgtc attcgtgagg
     7261 cgcaacgcgt gatcgaagag cgcgagcctc gtttgatcct gctcggcaca ccggaccagt
     7321 tcggcgagct acccgagggg atgatgttcg tccagatgtc gtgccagtcc gaaggcgctt
     7381 tggagcttta catagagcca gtcctggcca gcccccatct cgttgtggtc gggcattcgc
     7441 cgatggcaac gacattggtc gacctggcca cggtcctggg atggcgggcc gatcttttcg
     7501 aggcagctga attcacaacc gccgacgtca actcggcgtc cgtggtggtc atcgccaccc
     7561 agggtcatgg tgacgaggaa gccgtcgaac aggcggttgc aggcaagccc gccttcgtcg
     7621 gcctggtggc gtcccggaaa cggggcgagg ccgtactcgg ctatctcgcc gatcgcgggg
     7681 tgtcgaagga gcttctcgat gaggtcaggg tccctgtcgg attcgacctc gggcacacct
     7741 cccaccccga gatcgcggtt gcggtgctgg ccgaattggt cgagctgcgg gccaagggcg
     7801 ccctcagcaa acgggccgca gataccaagg tcgccattcc ggtcgctgag gcaatgaccc
     7861 cgggcacggc gatcgacctg gtgtgcggaa tgacggtgcc cgcggacaat accagccgcc
     7921 ccttcgagtt cgagggcgtg acctactact tctgctgtcc tgggtgccgg gtcgccttcg
     7981 agaaagaccc ggccaaattc atcaagaagg aggagctaca tgctgatcaa gcacgacttt
     8041 gaggtccccc tccccgtcga cagggtctgg gagttttgcc aggacattcc ccaggttgcg
     8101 tcctgcctgc ccggcgcatc tctgacggac caggtgggcc aagaccagta ccacggcaac
     8161 gtggcgattc acctgggccc ggtgcagatg cagttcgccg ggcgcgccga cgtgacgaaa
     8221 cgggacgaag ccagcaagac catcgtcatc gacgcctcag gcgccgacgc caaaggccgt
     8281 ggtcaagctg cactcgggct caccgcccga ctaaccccaa ccgcaaccgg caccaaggtt
     8341 tcggtggagc aagaccttca actctcgggc gctgcggcgc aatacgggcg gggcatggtc
     8401 tccgacttca gcgcagtgat gcttcgtgac ttcgccacga acatgcaggt ccgcatcgac
     8461 gcctttgatc gcggcctgtc acctgaccag attcagcagg cgaagagcgc cggcggtttc
     8521 agcctcgcca ttcaggccgc ctggatggcg ctcaagcggg tcggccggcg gttcttcctc
     8581 ccctaccagc ccaaccagat ctgaggagat gaccatgctt tggtggatag gaaacgcggt
     8641 gctcctcctg gtggtggtac cggttctggt cgccctgctc aacagggtgc tggccgcgtg
     8701 cgagcggatc agaggcgcct ccgatcagat cctcggcggg ggggtcggtc tcatcggtga
     8761 gctggacagg gtgcccgggc tgctcggcgt caccgacaag gccattgaag atgtatccct
     8821 cggggctgtc cgctatgccg gctcggtcgg caagttgctt ggttgacgag aggaaggacc
     8881 aatgcccgca accgcaatcg tgaccctcgt gatcgccacc ttgatcatcg ctgccgccgc
     8941 gctggggctg atgagggtga tcggccatct gagtgcgacg ttgaagaccc tgcgcgccct
     9001 aaccggaggg gtgggcgttg tcgcccaaca gaccagcggg gtgcccgccg ccgtggcgtc
     9061 gatcaacgcc aacctcaagc ccgtcagcga cttctgcgaa tcgatctgag gcacaaagaa
     9121 atgaacgagc tcttcgaagg aaacaccggc tggttggtcg gatacatcat cgggatcgtc
     9181 gtggtcctgg tggtggtagc cctcgtggtg ccgatcctgg ttctggccag gaggatcggc
     9241 aacgaagcac aactgatcga cgacagtctc aaggagtcag tggtgaacac agcggctctc
     9301 gccgacctgg ccaccaccaa caccatggcc gtcaacgtca tcgaaggttt gaagcggggc
     9361 cggctgaggt tgggaggatg accatgatct ttgcccttac cgaggaccag gagacgctct
     9421 ggcagattgc caactgggcg gggctcgtgg tggtagtcgc agtcgccgcc ctgctcacaa
     9481 tcctcgtcct actggtgcgc accatcgagc gcagagtggc cggcatcaag gccaccctga
     9541 ccgaggcgga agccaacacc tcagatgcgg cactgctcaa cacgacggct gcgggagtcg
     9601 atctggtgct ggcagagggt ctggagcatc atctcttcct gggccgggtg ctgggaaagg
     9661 ttcgctcatg aacagcctca tcgtcttcac agttgtcgac atcgtcctgc tgatcgccgg
     9721 cctggccatc tacctgtgga tcgtcggagg ccagctcacc cgggtagcca ccgggctcga
     9781 ggagtgcaac gacctggttc agaagatcaa ggacaacgcc gcgtccatcg aaggcaacct
     9841 cgagcacatc aaccgcaccg gcggtgtgat cgcaggggct ctccccctcc tctacgggat
     9901 ggccgagggc atcgtggttg gcgctaccta cgatccggaa aaagtcatcg agcggggccc
     9961 ggcgaagcct gcgtccggaa ctcgacgttc acggatgcat gagggggtcg
//
LOCUS       DBNHXU010000128         8834 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4008064, whole genome shotgun sequence.
ACCESSION   DBNHXU010000128 DBNHXU010000000
VERSION     DBNHXU010000128.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8834)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8834)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8834
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4008064"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..782)
                     /locus_tag="AC161X_03410"
     CDS             complement(<1..782)
                     /locus_tag="AC161X_03410"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4988626.1"
                     /translation="MFRRIAVVVVLAALSVLLFASVSAAALPPGGTFIDDDGDVHEGS
                     IEAIYAAGLTGGCDSRGIRYCPNDRVTRGQMAAFLARALNLPVSPDDRFTDDNSSDFE
                     AAINKVAAAGITLGCNPPANTRFCPDRPVTRAEMATFLVRSFPDLPDSGPDVFTDDNT
                     DVHQANINRIAAGGITVGCNPPTNDHYCPGTRVTRGQMATFLTRALHLQADKPPAQFP
                     IERVASFTTRFNCCEPRVTNIRAMARQIDDYVVMPGETFSVDR"
     gene            complement(827..2278)
                     /gene="zwf"
                     /locus_tag="AC161X_03415"
     CDS             complement(827..2278)
                     /gene="zwf"
                     /locus_tag="AC161X_03415"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013060581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HIR4988627.1"
                     /translation="MPDLEASLFVIFGGTGDLAERKLIPSLYRALAAQDAADRSVVLG
                     VSRHDIGDEAYRSWAERVLEASGAADKPARQWCQANFYHHAVEGSGAGYPELRKRIEA
                     IETERGLPGNRVLYLATPPQAFVPTIEGLGGSSLNESDGWTRIVLEKPFGTDLGTARE
                     LNRLVQSHFDESQIYRIDHYLGKATVQNLLAFRFTNPMFERLWNRDRVKHVEITVAET
                     LGIGSRADYYDRAGAVRDIVQNHMTQLLALVAMEPPNVFEADRIRDEKVKVMKAIAPV
                     AADDFVVAQYESGDMRDKAVLGYLNEEGVPPDSKTPTYVGIRLYVDSWRWQGVPFLLR
                     TGKRLPERFTRIKVTFREPALCVFHGRRDDCVMNPDVLLIILQPDEGFSLRFNVRSPD
                     DEAKLDSQALHFRYSEVYGELRDAYETLILDILEGDQTLFVRGDEVEASWEVYDPLLR
                     LDKDPIRYAAGTWGPSEMSQGMPIGGEQWMHRS"
     gene            complement(2294..3679)
                     /locus_tag="AC161X_03420"
     CDS             complement(2294..3679)
                     /locus_tag="AC161X_03420"
                     /inference="COORDINATES: protein motif:HMM:NF024748.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HIR4988628.1"
                     /translation="MSFVTRRVFTAVAAGSILLAALPLPAGATAASGLFTDDDGHPGE
                     VYLEWLADLGVVQGCNPPANTRICPDRTLNRAEAAKILVGAGQRSGFLPPIPASLPDR
                     FSDDNDLWSGSASRLANFLAYLGVISGCDPPANRLFCPSQELTRGQVAKMLVGVFDLT
                     APLSFASPWTDTAGRFYAESARIAAYHGLWDSSLGRFDGGESISRADFARAVVLSATG
                     DDLCPVTPFTAKRVDSLRRRFPGQIFTAYAYDTRTGCAYWMHQENRLRTASVFKVMVM
                     AGTLLEAQQDGRAVSSWEWSQLVPMITQSANNPVRALWRHFGGSPWFQRQADIYGLSQ
                     TSPVGDTGGVWGRTTTSAKDQADLLRQVLRGEWGPLEEQYRLKAWDLMTSVVSAQTWG
                     VTEGVPSGWTVAQKNGFAGHIANSVGFVRQPGGDDGYVIAVLSNGWSDWHKGVPVVSE
                     ISGWVSGRLAS"
     gene            3711..4130
                     /locus_tag="AC161X_03425"
     CDS             3711..4130
                     /locus_tag="AC161X_03425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020387081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6855 family protein"
                     /protein_id="HIR4988629.1"
                     /translation="MTAAPFTGSGTESDPWVLKTPSLGSEILMHRDEAHDPPVLVCTV
                     GSTVLFYDLRIIDDLHQMLKDEGDWVPLGNADEQKPAADGTVEAWARSGDNPIGGWYG
                     LKKGLRGRVGNYVPPVLEALGLAEVEHNARGNRMRAL"
     gene            complement(4132..4941)
                     /locus_tag="AC161X_03430"
     CDS             complement(4132..4941)
                     /locus_tag="AC161X_03430"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4988630.1"
                     /translation="MAQLSAKQRAELPNSAFAYVDSQGRRLLPINDEPHVRNALSRFN
                     QVKFEDEAAKERAFRKLLTAATKYGIAPIGFVARQLREAAAGESPAELPSGPVTLLLS
                     DIEGSTGLVHRLGEAYPELLAMVQGVIREAVDSHRGYEVDARADEFFAAFAHAADAVR
                     AALAIQRSLRDRQSAEEASVRVRIGLHSGSPARTETGYVGLPVNTAARVCNAGHGGQI
                     LISGTTRDELSTPGVDLVGLGVFDLRGLPRPVELFQVTVPDLPQEFPPPRV"
     gene            complement(5030..6430)
                     /locus_tag="AC161X_03435"
     CDS             complement(5030..6430)
                     /locus_tag="AC161X_03435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012873469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015851 - nucleobase transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HIR4988631.1"
                     /translation="MPIAGIRQLGGDMTDTVDRKQSSSGGSGLDGYFKLSERGTSVGT
                     EIRAGFTTFLVMAYIIFLNANILGEAGFDVPAVAAGTALVAGLLCVLMGVVANYPIAM
                     AAGLGINAAVAFGLVITDGLTQAGAMGVIVLEGLIVTLLVVVGLRQAIMNAVPLSLKR
                     AIGVGIGLFIMFIGFVDSGLIVPGVPIVDFAFPNTNGAWLTIIGLAITVVLFVRKVPG
                     ALVISILLTTVVAFIIGVSEFPSEFTATPSFATLGEFDLSNVFNQLGFVAAALTIFSF
                     MLTDFFDTVGTATGIAEQAGLTDEEGNIEDVGKVLFVDSLGAVAGGAAGVSSNTSYIE
                     SAAGVAEGGRTGITSIVVGLLFLVAIFLTPVAGLVPTQATAPVLILVGFLMSGLLKGI
                     DFEDIEEGFPALLAIVLMPFTYKITVGIGAGFVMWVFIKLVKGKAREVHPLMWVVAIA
                     FLIYFGQAVIGQAISA"
     gene            6538..6840
                     /locus_tag="AC161X_03440"
     CDS             6538..6840
                     /locus_tag="AC161X_03440"
                     /inference="COORDINATES: protein motif:HMM:NF019491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Dabb family protein"
                     /protein_id="HIR4988632.1"
                     /translation="MSHLRHTALLRFKPGTTDDQIQAVLDGLATMPGKMDFIRRYEFG
                     RDVGIMAGNPQVALVADFDSASDWHAYQDHPDHQVLIKEVISPILESITRVQYEVD"
     gene            complement(6900..7907)
                     /locus_tag="AC161X_03445"
     CDS             complement(6900..7907)
                     /locus_tag="AC161X_03445"
                     /inference="COORDINATES: protein motif:HMM:NF024091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988633.1"
                     /translation="MTALAEPASLAEPATVWERRRPGWMDVARKEMTDHLLSGRFIAL
                     LFILGLVALGTVYGAASGLRESAQQSTDAGLPAFLLLFTVNADPIPFSFMSFLVFMLP
                     IVGIAYGFDSISGERSQGTLPRLVSQPIHRDDVINGKFVAGLAVIGVTLTVVVMIVSG
                     VGIVALGIVPDASDVFRLITWLLASVLYVGVWLAMAMLASVVFRNAATSAIVTIGIWL
                     ALLFFAQLIFTLVASIIAPVDAADPFTQLSNARAEQTLSQISPVVLYDEASTALLVPF
                     TRTFQTLFTAEQADRAIPSELSFTQSVLLVWPQLVAMLAMTVLLFAVAYVKFMRQEVR
                     A"
     gene            complement(7904..>8834)
                     /locus_tag="AC161X_03450"
     CDS             complement(7904..>8834)
                     /locus_tag="AC161X_03450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016777512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988634.1"
                     /translation="VKNYEDRVAVAGIDFTVSPGEIFGVLGPNGAGKTTTILMLLGLT
                     EPSEGTVRVCGLDPAYDAIGVKRRVGYLPDAVGFYDNLTGRQNMRYTARLNELDPDAV
                     EDRISELLDEVGLAEAADYLVGTYSRGMRQRLGVADILVKDPDVVILDEPTTAIDPEG
                     VVEILALISRLAHRDGRAVLISSHLLNQVQQVCDRVGIFVDGEIVAQGSPEELAASLS
                     EGSSVYEFVVDATEEQVRAAVISGLPDLHEKIGVSAIGAGRWRVVLPESDISGVVKAL
                     VEADLSVRQIRDLGSNLDEIYRRYFHEHEGVSA"
BASE COUNT         1822 a         2778 c         2697 g         1537 t
ORIGIN      
        1 cgatcgacgg agaaggtctc gcccggcatc acgacgtaat cgtcgatctg gcgagccatc
       61 gcccggatgt tggtgaccct gggctcacag caattgaaac gagtggtgaa gctcgcaacg
      121 cgttcgatcg gaaactgcgc cggtggcttg tccgcctgga gatgtagggc tcgggtcagg
      181 aaggtcgcca tctgacctcg agtgacccga gtcccagggc agtaatgatc gttggtgggt
      241 gggttgcagc caacggtgat gccgccagcc gcgatccggt tgatgttggc ctgatggacg
      301 tctgtgttgt cgtcggtgaa cacatcgggg ccggaatcgg ggaggtccgg aaagctgcgc
      361 accaggaatg tggccatctc ggcgcgggtc accggccgat ccgggcaaaa acgagtgttg
      421 gcgggagggt tgcacccgag cgtgatcccg gcggcggcca ccttgttgat ggccgcctcg
      481 aagtcagagc tgttgtcatc ggtgaatcgg tcatcaggcg atacggggag attcagcgca
      541 cgagccagga aggcggccat ctgtccccgg gtgacccggt cgttggggca gtacctgatg
      601 ccccgtgagt cgcagccacc ggtcagccct gcggcataga tcgcctcgat cgatccctca
      661 tgcacgtcgc cgtcgtcgtc gatgaaggtt ccaccgggag gaagggctgc ggccgacacg
      721 ctcgcgaaaa gcagcactga gagggcggcc agcacaacga ccactgctat ccggcgaaac
      781 atcgtgcgag tcttccatgt attcgcgcca acaccaagcg ttgacctcac gagcggtgca
      841 tccactgttc accaccgatc ggcatgccct ggctcatctc agacgggccc caggtgccgg
      901 ccgcgtagcg gatcgggtcc ttgtcgagtc ggagcagcgg gtcgtagacc tcccacgagg
      961 cctcaacctc gtctccccgc acaaacagtg tttggtcgcc ttcgagaatg tcgaggatga
     1021 gcgtctcgta tgcgtcccgc agttccccgt atacctccga gtagcggaag tgcaaggcct
     1081 gggagtcgag cttcgcctca tcatccgggg acctgacgtt gaatctgagc gaaaacccct
     1141 cgtccggctg gaggatgatc aacagaacat cgggattcat cacacagtca tcccgcctgc
     1201 catggaaaac gcatagggcg ggctcgcgga acgtcacctt gatgcgggtg aagcgctcgg
     1261 gaaggcgctt accggtgcgc aggagaaagg gaacgccctg ccagcgccag gaatccacat
     1321 acagccgaat gcccacataa gtcggtgtct tcgaatctgg cgggaccccc tcctcgttga
     1381 ggtagccgag cactgcctta tccctcatgt cccccgactc gtactgggcg accacgaagt
     1441 catcggccgc gacgggggcg attgccttca tcactttgac cttctcgtca cggatccgat
     1501 cagcctcgaa gacattcggc ggctccatgg cgacgagggc caacagctgg gtcatgtggt
     1561 tctgaacgat gtcccgcacc gcaccggcgc ggtcgtaata gtcggctctg cttccgatgc
     1621 cgagcgtctc ggcaactgtg atctcgacat gcttgacgcg atcccgattc cacagccttt
     1681 cgaacatcgg gttggtgaag cggaaggcca ggagattctg aacggtcgct ttgcctaagt
     1741 agtggtcgat gcgatagatc tgggattcgt cgaaatgcga ctggacgagg cgattgagct
     1801 cgcgggctgt ccccaggtcg gtgccgaagg gcttttcgag cacgatcctg gtccaaccgt
     1861 cgctttcgtt gagactcgag ccgcccaacc cctcgatggt cggaacgaag gcttggggtg
     1921 gggtcgcaag gtagagcacc cggtttccgg ggaggcctcg ctccgtctcg atggcctcga
     1981 ttcgcttccg cagctctgga tacccggcac cgcttccttc gactgcatgg tgatagaagt
     2041 tggcctgaca ccattgcctc gccggcttat cagcggcgcc gctcgcttcc aacaccctct
     2101 cggcccaact ccggtaagcc tcatctccta tgtcgtgccg ggagacaccc agcaccaccg
     2161 atcggtcggc agcgtcctgg gcagccagcg cccggtatag agatgggatc agcttgcgct
     2221 cggcgaggtc acccgttcca ccgaatatga cgaacaggct tgcttcgagg tcaggcatgt
     2281 agtcgttcgt ttctcacgag gcaagcctcc cagaaaccca gcccgaaatc tcgctcacga
     2341 ccgggacgcc cttgtgccag tcggaccagc cgttgctcag gacggcgatc acgtacccgt
     2401 cgtcacctcc gggttgccgt acgaacccga ccgagttggc gatatgcccc gcgaacccat
     2461 tcttctgagc cacagtccaa ccagagggaa cgccctcggt gaccccccag gtttgggcgg
     2521 agacaaccga agtcatcaga tcccaagcct tcagacgata ttgctcctcg agcgggcccc
     2581 actcgccgcg caagacctgc cgcaacaggt cggcctgatc cttggccgag gtggtggtgc
     2641 gaccccacac cccgccggtg tcaccgaccg gggacgtctg actcaaccca tagatgtcag
     2701 cctggcgttg aaaccatggg gatcccccga agtgcctcca cagagcgcga accgggttgt
     2761 tggccgactg cgtgatcatt ggcaccagct gactccactc ccaggaggag accgcccgcc
     2821 cgtcctgctg ggcctcgaga agcgtccctg ccatcaccat caccttgaat acagatgcgg
     2881 tgcggagacg attctcctgg tgcatccagt aggcgcagcc ggtgcgggtg tcgtaggcat
     2941 aggcggtgaa gatctggccc gggaaccgcc gacgaagcga gtcgacccgt ttggcggtga
     3001 atggggtgac aggacagagg tcatcgcccg tagcggatag aaccacagcc ctggcgaaat
     3061 cggctctcga gatcgattcg ccaccatcga aacgccccag gctcgaatcc cacaacccgt
     3121 ggtaggcagc gatccgtgcc gactctgcgt aaaagcgacc cgcggtgtcc gtccagggtg
     3181 aagcgaaaga caggggcgcc gtgagatcga agaccccgac cagcatcttg gccacctggc
     3241 cgcgggtcaa ttcctgagaa gggcagaaaa gccggttcgc aggtggatca cagcccgaga
     3301 tcaccccgag ataggcgagg aagttggcga gtcgcgaggc actgccactc cagaggtcat
     3361 tgtcatcgct gaatctgtcg ggcagggagg ccggaattgg tggaagaaac ccggaccttt
     3421 gccctgctcc caccaggatc ttggctgcct cggcccggtt cagagtgcgg tcggggcaga
     3481 tgcgggtgtt ggccggtggg ttgcatccct ggaccacacc caaatcggca agccactcga
     3541 gatagacctc gccgggatgg ccatcatcat cggtgaagag ccctgatgcc gcagtggcac
     3601 cggcggggag tggcagcgcc gcgaggagga ttgagcctgc ggctactgcg gtgaagaccc
     3661 tgcgggtcac gaacgacatc gcgggattat cgccgagtag tgtcgatttg gtgacggcgg
     3721 caccattcac cgggagcggg accgagagcg acccgtgggt gctgaaaacc cccagccttg
     3781 gatctgagat cttgatgcac cgggacgagg ctcacgaccc gccggtcctg gtttgcaccg
     3841 tcggcagcac ggtcctgttc tacgacctgc ggatcatcga cgacctccat caaatgctca
     3901 aggacgaggg agattgggtt cccctgggca acgccgatga gcagaaaccc gccgcagacg
     3961 gcacggtcga agcctgggcc aggtcgggag acaacccgat cggtggttgg tacggcttga
     4021 agaagggcct ccggggcagg gtgggcaact acgtcccacc cgtgctcgag gcgctcggcc
     4081 tggccgaagt cgagcacaac gcacgcggca atcggatgcg agccctttga cctagacccg
     4141 gggcggcggg aactcctggg gtaagtcggg aacggtcacc tgaaacaact ccacaggacg
     4201 gggcaaacca cgtaggtcga agacgccaag cccgacaagg tcaaccccag gcgtgctcag
     4261 ctcgtcgcgg gtcgtccccg aaatcagaat ctgccctccg tgaccggcat tgcagacacg
     4321 agccgcagtg ttgacgggca gtcccacata gcccgtctcg gtgcgagccg ggctcccact
     4381 gtgaagaccg atccgaaccc gaactgaagc ctcctcggcc gactggcgat ctcgcagcga
     4441 acgctgaatg gcgagggccg ccctaacggc gtccgcggcg tgagcaaagg cggcgaagaa
     4501 ctcgtcggcc ctcgcatcga cctcataacc acgatgggag tcgactgcct cgcggatgac
     4561 cccctgcacc atggccagca gctctggata agcctcgccg agccgatgga ccaatcccgt
     4621 cgacccttcg atgtcgctaa ggaggagtgt gactgggccc gatggaagct ccgccgggct
     4681 ctcccccgcc gcagcctccc tcaactgccg ggccacgaat ccgatgggag caatcccgta
     4741 tttggtagcc gcagtgagca gcttcctgaa ggcccgctcc ttggccgcct cgtcctcgaa
     4801 cttgacctgg ttgaaacggg aaagggcatt gcgcacatga ggctcgtcgt tgatcggcag
     4861 caggcgccgg ccctgagaat cgacgtaggc aaaggcgcta ttcggaagtt ctgcgcgctg
     4921 cttggcggag agttgagcca tgtggaggcc gaaactacgc cgtgccgact gagtccagac
     4981 gcgtcgaggg cggcgcccac ccggcgccgc cctcagacat ttgctcggtt caggctgaga
     5041 ttgcttggcc tatgaccgcc tgaccgaaat agatcaagaa ggcaatggca accacccaca
     5101 tcagcgggtg cacctcgcgc gcctttcctt tgaccaactt gatgaacacc cacatcacga
     5161 atccggcacc gatcccgacc gtgatcttgt aggtgaacgg catgagaacg atggcgagca
     5221 gagccggaaa gccctcctcg atgtcctcga agtcgatgcc cttgagcaac ccactcatca
     5281 agaagccaac caggatcaac actggcgccg tcgcctgagt cggaaccaga ccggcgaccg
     5341 gtgtgaggaa gatggccacc aggaacagca gaccgacaac gatcgacgtg atgccggtac
     5401 gaccgccttc ggcgacgccg gccgcactct cgatgtagct ggtattggag ctgactcctg
     5461 ccgctccacc cgcaactgca cccagggagt cgacgaacag caccttgccc acgtcctcga
     5521 tgtttccctc ctcatcggtg agaccggctt gttcggcaat cccggtggcc gttcccaccg
     5581 tgtcgaagaa gtcggtcagc atgaaactga agatggtgag cgctgccgcc acaaagccga
     5641 gttggttgaa cacattcgac aggtcgaact caccgagggt tgcaaagctc ggtgtggcag
     5701 tgaactcact agggaattcg ctgaccccga ttatgaacgc gaccacggtc gtgagcagga
     5761 tgctgatcac cagcgctccc gggacttttc ggacgaagag caccacggtt atcgcgagcc
     5821 cgatgattgt cagccaggct ccgttggtat tgggaaacgc gaagtcgacg atcggcacac
     5881 cgggcacgat caatccactg tcgacaaaac cgatgaacat gatgaacagc ccgatgccga
     5941 ctccaatcgc ccgcttcagg ctcaggggta cggcattcat gatggcctga cggagaccga
     6001 ccacaaccag gagtgtgacg atcagacctt cgagcacgat gacgcccatt gctccggcct
     6061 gagtcagtcc atcggtgatg acgaggccga aagccacagc tgcgttgatc ccaagtccgg
     6121 cggccattgc aatgggatag ttggcaacca cccccatcag gacgcagagc agaccggcga
     6181 ccaatgctgt gccggcagcc actgccggaa catcgaagcc ggcttcgccg aggatgtttg
     6241 cgttgaggaa gatgatgtac gccatcacca ggaacgtcgt gaatccggcg cggatctcag
     6301 tccctaccga tgtacctcgc tctgacagct tgaagtagcc atcgaggccg gatccgccgg
     6361 aactcgactg cttccgatcg accgtgtcgg tcatatctcc tcccaattga cggatgccgg
     6421 cgatcggcaa ccaatttcag accaaaagta gtgaggagtg gttacaccct gccgtgcgaa
     6481 ctcggcattt gtttccggga aatcgcgggc tttgcccgac tctggttgac tccgggcatg
     6541 agtcatctac gtcacaccgc cctgctccgc ttcaagccgg gaacgacgga cgatcagatc
     6601 caggcagtgc tcgacgggct tgccaccatg ccaggcaaga tggatttcat ccgtcgctac
     6661 gagttcgggc gggacgtcgg gatcatggcg ggcaatcctc aggtcgccct agtcgccgac
     6721 ttcgattcag ccagcgactg gcacgcgtat caggaccatc ccgatcacca ggtcctgatc
     6781 aaagaggtga tcagcccgat cctggagtcg attacccgag tccaatacga ggtcgactga
     6841 ttctctgcgc tcgtagcgcc acatgtggcg caacgtacct caggtaaggg ttctgggcct
     6901 caggccctga cttcctgtcg catgaatttg acgtaggcga ccgcgaacaa cagcacggtc
     6961 atggccagca tcgcgaccaa ttgcggccag accagcagca cgctctgggt gaaggagagc
     7021 tccgacggaa tggctcggtc cgcctgctcc gcggtgaaca gagtctggaa ggttcgcgtg
     7081 aagggcacca acaacgcagt cgacgcctcg tcgtagagga cgaccggtga gatctgggac
     7141 aaggtttgct cggcccgtgc gttgctgagt tgggtgaacg gatcggccgc atcgacgggg
     7201 gcaatgatgc tcgccaccag agtgaagatc agctgggcaa agaacaacag ggccagccag
     7261 atcccgatgg tgacgatcgc cgacgtcgca gcgttgcgaa acaccacgct ggccagcatt
     7321 gccatagcca gccagacgcc aacgtagagg actgaggcga ggagccaggt gatcagtcgg
     7381 aatacgtcgc tcgcgtcggg gacaatcccg agggcaacaa tccccacgcc ggacacgatc
     7441 atgacgacaa ccgtgagcgt caccccgatc acggccagac cagcaacgaa cttgccattg
     7501 atgacgtcat cgcgatggat gggctgcgag accaggcggg gcagggtccc ctgcgaccgc
     7561 tcgccgctga tcgagtcgaa gccgtaggca atccccacta tcgggagcat gaacacgaga
     7621 aacgacatga aggagaatgg gatcgggtct gcgttcaccg tgaagaggag gaggaaggca
     7681 ggaagacccg cgtcggtgga ctgttgcgcc gactcccgaa gcccgctcgc cgccccatag
     7741 acggttccca gggcgaccaa gccaaggatg aagagcagtg cgatgaaccg acctgacaga
     7801 agatggtcgg tcatctcctt tcgagcaaca tccatccaac ccggtcgtcg ccgctcccaa
     7861 accgtcgccg gctctgcaag acttgccggc tctgcaagag cggtcatgcg ctcacccctt
     7921 cgtgctcatg gaaatagcgc ctgtagatct cgtcgaggtt cgagccgaga tctctgatct
     7981 gcctgacact gaggtctgcc tctacgagtg ccttgacgac ccctgaaata tcactctccg
     8041 ggaggaccac acgccacctc ccggcgccga tggcagagac cccgatcttc tcgtgcaaat
     8101 cgggcaggcc gctgatcact gcagcccgca cctgctcctc ggtggcgtcg actacgaact
     8161 cgtataccga actgccctcg gacaacgacg ctgcgagctc ctcaggcgag ccctgggcga
     8221 cgatctcccc gtctacgaaa atcccgaccc gatcacacac ttgctgcacc tggttgagca
     8281 ggtggctgga aatcaatacc gccctgccgt cacgatgggc cagtcgtgag atgagggcaa
     8341 ggatctcaac gacaccttcc ggatcgatcg cggttgtggg ctcgtccagg atgaccacat
     8401 caggatcctt gaccaggatg tcggcaaccc cgagacgctg acgcataccc cgggagtaag
     8461 tgcccaccag atagtcagcc gcctcggcga ggccgacctc gtccaacagc tccgatatcc
     8521 ggtcctcgac cgcatcgggg tccagttcgt tgagacgagc tgtgtaccgc atgttctgtc
     8581 gcccggtcag gttgtcatag aacccgacgg cgtcgggcag atagccgacc cggcgtttca
     8641 ctccaatggc gtcataggcc ggatcgagac cgcacacccg aaccgtcccc tcgctgggct
     8701 cggttaaacc gagcagcatg aggatggtcg tggtcttccc ggcgccgttg ggcccgagaa
     8761 ccccgaagat ctcacccggt gaaaccgtga agtcgatccc ggccacggca accctgtcct
     8821 cgtaattctt gacc
//
LOCUS       DBNHXU010000129         2068 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5398640, whole genome shotgun sequence.
ACCESSION   DBNHXU010000129 DBNHXU010000000
VERSION     DBNHXU010000129.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2068)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2068
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5398640"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1205
                     /locus_tag="AC161X_03455"
     CDS             24..1205
                     /locus_tag="AC161X_03455"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR4988635.1"
                     /translation="MFDNGTSIWDTLDFGSPEFVEVEELRAGILADEAEISAIRARQI
                     ARLRRLDVLQVDTADGARTMADWVTANLDISPQTAGRLVRIARSQQPDIEERLVLGEY
                     GVDRAAFLCRLRELGAPEEVIVNSSDYSLGHLYGLIDRLRRIDELSEGMSFEDRYLVL
                     QPSLDESAYRIWGQLPGVDGRIVEKALNNRETELPVLPDQRSGQRRADALTSICMDSL
                     TSSAEDGKGRAVTVAEVFVDASLAAPTFGEAGVTLSSGPRVGPNALDEILCTGRIRVI
                     VEDADLRPIGVSDLGEAIPPAVRAAVLHRDQGQCQIDGCRSKYRLQPHHIRPRSRGGD
                     HDPVNLVTLCWYHHHVAIHMLGMILEPGSPPHRRRLIRLPNHDPPQTTHRSVTAFATL
                     N"
     gene            complement(1237..1416)
                     /locus_tag="AC161X_03460"
     CDS             complement(1237..1416)
                     /locus_tag="AC161X_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988636.1"
                     /translation="MVVVLGVVVVVDGVVVVGSWEVVGATDVVVDWPCPLAVQEISAR
                     PRVKEKTAGVDRVTP"
     gene            1436..>2068
                     /locus_tag="AC161X_03465"
     CDS             1436..>2068
                     /locus_tag="AC161X_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988637.1"
                     /translation="MTPPTGAVIATGEGWELTDQEFSDLVDFVAVSMQQSFPEPVAVE
                     VLGLNGVTDLGEPGLEYITPLQWDLIRALGLVSDDQASLDEVNELRRQRVRGTCCLAA
                     EEGLTVQVEDAGSEELTKLVIVHELVHALLTQSPPQGIVPNEAFDEPVDVVTAAAEGV
                     PQWVALIYHASLTAAEQDDMADELPIIRDVDLESGIPDAAVELLTFGYTRG"
BASE COUNT          405 a          645 c          615 g          403 t
ORIGIN      
        1 ccgcctaagt acgttacgaa catatgttcg ataacggcac ttcgatctgg gacactctcg
       61 atttcggatc gcccgagttc gtcgaggtcg aggagttgag ggccggaatc cttgcggacg
      121 aggcggagat ctcggcgatc agagcccgtc agatcgcgag gttgcgtcgg ctggacgtat
      181 tgcaggtcga caccgccgat ggggctcgga cgatggccga ctgggtcaca gccaatctgg
      241 atatctcccc acaaaccgcg ggccgcttgg tgcgaattgc tcgctcacag cagccagata
      301 tcgaagagcg gctggtcttg ggcgaatacg gagttgatcg agctgccttc ctttgtcgcc
      361 tccgtgagtt gggtgctccg gaggaggtca tcgtcaactc atcggattac agcctcgggc
      421 atttgtatgg gttgatcgat cggctgcgac ggattgacga attgtccgag ggaatgagct
      481 tcgaagaccg atatctggtt cttcagccct cgctcgatga gtcggcgtat cgaatatggg
      541 gtcaactgcc cggtgtggat ggtcggatcg tggagaaggc gctcaataac cgagagaccg
      601 aactgccggt tctgcccgat caacgatccg gacaacggcg ggcggacgcc ctcaccagca
      661 tctgcatgga ctcgctgacg agcagtgccg aggatggcaa gggtcgggcg gtgacggtcg
      721 ccgaagtatt cgtcgacgcc agcctcgcag caccaacatt cggcgaagct ggggtcacgt
      781 tgtcctccgg gccgcgggtc gggcccaatg cgctggatga gatcctgtgc accggcagga
      841 tccgagtcat cgtcgaagac gccgatcttc gaccgattgg tgtctccgac cttggagaag
      901 ccatcccgcc ggccgtgcgc gccgcggtgt tgcatcgaga ccagggccaa tgccagatcg
      961 atggctgcag gagcaaatac cggctccaac ctcaccacat tcggccgcgt tcacgaggcg
     1021 gcgaccatga tccggtcaat ctcgtcacgc tttgttggta tcaccaccac gtagcaattc
     1081 acatgctggg catgatcctc gaaccgggga gtccgccaca tcgtcgccgt ctgatacgtc
     1141 tgcccaacca cgatccaccc cagaccactc accgatccgt cacagccttc gccaccctca
     1201 actgaccttt cgatgggatc cgggtacctc ctattgtcac ggagtgacac ggtccacgcc
     1261 ggctgtcttt tccttgactc ttggtcttgc gctgatctcc tgcaccgcaa gcgggcaagg
     1321 ccaatcgacc acgacgtcgg tcgcaccgac gacttcccaa ctcccaacca cgaccactcc
     1381 atcgaccacg accaccaccc cgagtacgac aaccaccgtt gaaagcgttt ctccgttgac
     1441 gccgccgacg ggagccgtga tcgccaccgg ggaaggttgg gagctgaccg atcaggaatt
     1501 ctccgacctc gtcgacttcg ttgcggtctc gatgcagcag tcgttcccag aaccagtcgc
     1561 tgtcgaggtg ttggggctca acggggtcac cgaccttggt gagccaggtc tcgagtacat
     1621 cacacccctc cagtgggacc ttatccgggc gctcggattg gtctcggatg atcaggcctc
     1681 gctcgatgag gtgaatgaac ttcgccggca acgggtacgc ggcacctgtt gcctcgcagc
     1741 ggaggaagga ctcaccgttc aggttgaaga cgccggctca gaggaattga ccaagctcgt
     1801 gatcgttcat gagctggtgc acgccctgct cacgcaaagc ccgccacagg ggatagtccc
     1861 caacgaggct ttcgacgagc cggtagatgt cgtcaccgcc gccgccgagg gcgtgccgca
     1921 atgggtggcc cttatttatc acgcttcgct cacagccgcc gaacaagacg acatggcaga
     1981 cgagctgcca atcatccggg atgtcgactt ggaatccggg attccggacg ccgccgtcga
     2041 acttctcacc tttggataca cccgcggc
//
LOCUS       DBNHXU010000130         4781 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4207514, whole genome shotgun sequence.
ACCESSION   DBNHXU010000130 DBNHXU010000000
VERSION     DBNHXU010000130.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4781)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4781)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4781
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4207514"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1438)
                     /locus_tag="AC161X_03470"
     CDS             complement(<1..1438)
                     /locus_tag="AC161X_03470"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding SpoIIE family protein phosphatase"
                     /protein_id="HIR4988638.1"
                     /translation="MVQLLRRRKTEEAPSEAPETSLSPGTRVIDAVPIPPGDPLETAL
                     LSTGAPLELASVEFESEALQTMRERGVELIVPLIGQGELLGALYLGPRLSDQPYSTDD
                     RHLLGSLASQVAPAMKVAQLVREQQAEAKERERIQQELQVAALIQQTLLPKELPAIPG
                     WHVDAFYRPARAVGGDFYDFINLEDGRLGVVIGDVTDKGVPAALVMATCRSMLRAAAQ
                     RHNSPSAVLADVNEALVPEIPPAMFVTCLYAIIDTNEGEIVLANAGHNLPYVRSSGGV
                     VELRATGMPLGLMPGMPYDERTYRLERGDVMVLTSDGITEAHNVEGEMYGFGRLKGRV
                     AIRPSNDDMVAALVGDLESFTGADAEQEDDITLVVVRRTASAKESAETFRSGDAEVLA
                     RFSVPSEEGNERIAITKVSEAVGHLGLEEARLERLKTAVGETVMNAIEHGNENRAEIP
                     VEIEVRATAASVSVRVSDQGGDKVIPEAE"
     gene            complement(1450..2646)
                     /locus_tag="AC161X_03475"
     CDS             complement(1450..2646)
                     /locus_tag="AC161X_03475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988639.1"
                     /translation="MTGKPSESRTSRWLPALVVVAGFSMLVFNLIAGSEESDLSGYFL
                     LAASAAFTLVGALIIARTDGNRVGWVSSAIGLALLGSGVVGYFSDQGDVAAGAIGGAL
                     WLSWFALTGLLMLWFPTGHPVSPKWRWVGWLGFLVLLTALTYVVSDQVCLEGGDPCAG
                     WVDNPIGISAVPNPEFGPLAGFNFSVLGVFVLLSTVSLIVRSVRARGVERLQMKWFLF
                     AVVFVILVVPFQEAVVARLSIPEWLGELVFGIAILLLPVSIGVAVLRYRLYDIDRIVS
                     RTVSYTVVVVVLAAIYVGVVTWLTTLLPDQSELVVAATTLAVATLFNPVRKRVQTWVD
                     RRFNRSRYNTQRVMDAFAASLRDQVDAGQVVEGWMGVVRETMQPASVSVWTRQAFRND
                     SETVEG"
     gene            2780..>4781
                     /locus_tag="AC161X_03480"
     CDS             2780..>4781
                     /locus_tag="AC161X_03480"
                     /inference="COORDINATES: protein motif:HMM:NF024589.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4988640.1"
                     /translation="MISSCAVLVTLRYRPTIGDATIDIRLLGPPRIEKAGTPIDVDTR
                     KAIALLAYLTVETTATRDRLASLLWPESNTERSRATLRRTLSSLRTAVGHEIVTADRI
                     SVALAEDTPSDIRSFAAELERTDHHDHDRGDVCGLCIPHLERATALYRGDFLEGFSMR
                     DAAEFEDWARTVTESLRIQAGVAFQRLAMAKAASGDYSGALRSVHSWIELDSLHEPAY
                     RLLMLLHAWDGDRPGAVEAYRRCVAVLDQELGVPPLEETTELYEAILDEDLPPAPGVR
                     RRVKAQAIAVAQREDMIDREKETATLLQAMENLAVGGKGIVVTGSAWMGKTRLLEELG
                     SSARASGRSALSGRAFRTEQDLPFGVVAQLLRSAAPLIEKNLDQLPTWAMGEVARLVP
                     EFQQARATPESADPFGELRLFDGVFTVLSQLSRIHPLVVMVDDVQWLDPASNGLISYV
                     ASRLETAPMLLVLALRTGEPLSAMTGDLISDAQDRIDLQPLTIDQLAGITGTIESAKL
                     LHQRTGGVPLLVTEELSDRRERGPDGVGMARYMESRLREISDLGRQVATAAAVLNGVC
                     DASLLRETSGRTEDEVVDGVEELVAAGLLRELPESDDLAFNLDALQRLTYDSTSLIRR
                     RLLHRRAASALSERPHAATDPRLAAAVAFQLEGAGSDESPEWY"
BASE COUNT          934 a         1570 c         1443 g          834 t
ORIGIN      
        1 tttcggcctc gggaatgacc ttgtcaccgc cctggtcgct gacccggacc gagacactgg
       61 cggccgtagc ccgaacctcg atctcgacag ggatctcggc gcgattctcg tttccgtgct
      121 cgatggcatt catcaccgtc tccccgacgg ccgtcttgag acgctcgagc cgggcctctt
      181 ccaagcccag atggcccacg gcctcgctca cctttgtgat cgcgattcgc tcgttgccct
      241 cttcgctggg aacgctgaac ctggccagga cctcggcgtc gccggatcgg aaggtctccg
      301 cagattcttt ggcggaggcg gtgcgccgga cgaccacgag tgtgatgtcg tcttcctgct
      361 cggcatcggc tccggtgaag ctctcgagat cgcccactag cgcagcgacc atgtcgtcgt
      421 tggagggcct gatggcaacc cgtcccttga gacgaccgaa tccatacatc tcgccttcga
      481 cgttgtgggc ttcggttatg ccgtcgctgg tgagcaccat cacgtcgccg cgctccagac
      541 ggtaggtgcg ctcgtcatat ggcatacccg gcatcaatcc cagtggcatc cctgtggctc
      601 ggagctcgac cacgccgcca ctgcttctca cataaggaag gttgtgaccc gcgttggcaa
      661 ggactatctc gccctcgttg gtgtcgatga ttgcgtagag gcaggtgacg aacatggcag
      721 gtggaatctc ggggacgaga gcctcgttca cgtcggcaag caccgccgat ggcgaattgt
      781 gcctctgcgc cgcggccctc agcattgacc ggcaggtcgc cataaccaac gccgcaggga
      841 cacccttgtc ggtgacgtct ccaataacca ccccaagccg accgtcctcg aggttgatga
      901 agtcgtagaa gtcaccgccc acggcgcgag ccgggcggta gaaggcatcg acgtgccaac
      961 cggggatcgc cgggagctct tttggcagaa gggtttgttg aatcagtgcc gcaacctgca
     1021 gctcctgttg gatccgctct ctctctttcg cctcggcctg ttgctccctg accagctggg
     1081 caactttcat cgccggcgcg acctgtgagg ccagactccc gaggagatgc cggtcgtcgg
     1141 tcgaataggg ctggtcggaa agacggggcc cgaggtagag ggctcccaac agctcgccct
     1201 ggccgatgag gggcacgatc agctcgacgc cccgttcccg catcgtctgc aaggcctcgg
     1261 attcgaactc gaccgaagcc aactcaaggg gggcacccgt ggaaagcagt gctgtttcca
     1321 agggatcgcc tggtgggatc ggcaccgcgt cgatgacccg ggttccgggt gaaagcgagg
     1381 tttcgggggc ctcagatggg gcttcctcgg tcttgcgacg tctcaacaac tggaccattg
     1441 gctcactttc tagccctcga ccgtctcgga atcgttacga aatgcctgtc tcgtccaaac
     1501 cgagaccgaa gccggctgca tggtttctct gaccactccc atccacccct caaccacctg
     1561 cccagcatcg acctgatccc gcagggatgc agcaaaagcg tccatcaccc gctgcgtgtt
     1621 gtagcgggac cgattgaacc ggcgatccac ccatgtctga actctcttcc ggacaggatt
     1681 gaagagggtt gccaccgcca aagtcgtggc ggccaccacc agttcagact ggtcggggag
     1741 cagggtcgtc aaccaggtga cgacgccgac gtaaatggca gcgagcacca ccacaaccac
     1801 cgtgtacgac actgtccgcg acacgattcg atcgatgtcg tagagccgat aacggagcac
     1861 agcgaccccg atggacaccg gcaacaagag aattgcgatg ccgaacacca gctcgccgag
     1921 ccattcaggg atcgacagcc gtgcgacaac ggcctcttgg aaggggacca ccaggatgac
     1981 gaaaacgacc gcgaagagga accacttcat ctgaagccgc tccaccccgc gtgccctcac
     2041 cgatctgact atcagcgaca cggtggagag aagcacgaac accccaagca ccgagaagtt
     2101 gaagcctgcc aacggcccga attcggggtt gggcacagcg ctgatcccga tcgggttgtc
     2161 gacccaaccg gcgcagggat cgccgccttc gaggcagacc tgatcggaca cgacgtaagt
     2221 cagagccgtg agcagcacga ggaaaccgag ccaaccgacc caccgccact ttggtgacac
     2281 cgggtggccg gtcgggaacc agagcatgag aagtcctgtg agagcgaacc acgagagcca
     2341 caaggctccc ccaatcgccc ccgccgccac atcgccctgg tcggagaagt agccaacgac
     2401 gccgctaccg agcagcgcca gaccgatcgc cgaggacacc catcccaccc ggttgccgtc
     2461 ggtgcgggcg atgatcaaag cccccaccaa ggtgaaggcg gcagaggcgg cgagcaggaa
     2521 gtagccagag aggtcactct cctccgaccc cgcaatcaag ttgaacacca acatcgaaaa
     2581 gccggcgaca accaccaggg caggcagcca tcgagaggtt cgactttctg agggcttccc
     2641 ggtcacgttt cccacccctc gtcagcggaa ggggtatgtg cgccaggatc catcggcggc
     2701 agcatgccat tgcttcgtta cgtaaccgca accggctcaa cacattgcca gagccgccca
     2761 cgggtcaaga agccattccg tgatctcgag ttgcgcagtt ctcgtaacgc tgcgatatcg
     2821 tccgacaatc ggagacgcga ccatcgacat caggctcctc ggtccaccgc ggatcgaaaa
     2881 ggccggaacg ccaatcgacg tagacaccag gaaggcgatt gccctcctcg cctatctgac
     2941 cgtcgagacc acagccaccc gcgaccggct ggcgagcctc ctgtggcccg aatccaacac
     3001 cgagcgctcc cgggccacct tgcggcgcac actgtcctca ctccgcaccg ccgtcggcca
     3061 tgagatagtg accgccgacc ggatcagcgt ggccctagct gaggacaccc ctagcgatat
     3121 ccgctctttc gccgccgagc tcgaaaggac cgaccaccac gaccacgacc ggggtgatgt
     3181 ctgcggtttg tgcattcccc acctcgagcg ggccaccgcg ctctaccggg gtgactttct
     3241 ggaagggttc tcgatgcggg atgctgccga gttcgaggac tgggccagaa cggtcaccga
     3301 gtctcttcgg atccaggccg gggttgcctt tcaacgtctg gcaatggcga aggcggcgtc
     3361 aggcgactat tccggtgccc tccgatcggt tcacagctgg atcgagctcg attccctcca
     3421 cgagcccgcc taccgccttc tgatgctgct ccacgcctgg gatggcgacc gtcccggcgc
     3481 ggttgaggcc taccggcgat gcgtcgctgt cctcgaccag gaactaggag ttcccccttt
     3541 ggaggagacc accgagctct atgaggcaat cctcgacgag gacctcccgc cggctcccgg
     3601 cgtcagacgc agggtcaaag cacaagcaat agcggtggca caacgtgagg acatgatcga
     3661 ccgggagaaa gagactgcca ccctgcttca ggcgatggag aaccttgccg tggggggaaa
     3721 ggggattgtc gtcaccggat cggcctggat gggaaagacc cgccttctcg aggaactcgg
     3781 gtcgtcggcg agagcttcag ggaggtcggc actctccgga cgagccttca ggacagagca
     3841 ggatctccct ttcggagtgg ttgctcaatt gctccgctcg gcggctccgt tgattgagaa
     3901 gaatctcgac cagcttccaa cctgggcgat gggagaggtg gcgcgcctgg tccccgagtt
     3961 tcagcaggct cgagcaacgc cagagagcgc cgacccattc ggagaactgc ggttgttcga
     4021 cggtgtcttc accgtcctca gccaactgtc ccgtatccat ccgctggtgg tgatggtcga
     4081 cgacgtccag tggctggatc cggcgtccaa tgggctcatt tcttatgtcg ccagccggtt
     4141 ggagactgcg ccgatgctcc tcgtccttgc cttgcggaca ggggagcccc tgtctgcgat
     4201 gaccggggac ctgatctcgg acgcccagga caggatcgac cttcaacccc tgaccatcga
     4261 ccagttggcc gggatcacgg gcacgatcga atcggccaag ctgctgcacc aacgcaccgg
     4321 tggagtcccg cttctggtca cagaagagct atcggatcgc cgtgaacggg gtcccgatgg
     4381 agttggtatg gctcgataca tggagtcccg cctcagagag atcagcgacc tgggccgtca
     4441 ggtcgccacg gccgccgcgg tgctcaacgg tgtctgcgac gcctcactcc tccgtgagac
     4501 cagtggccgc accgaagacg aggtggtcga tggagtcgag gagttggtgg cggccggact
     4561 gctccgcgag ctcccagaaa gcgatgatct tgccttcaac ctcgacgctt tgcagcggct
     4621 cacctacgac tcgacatccc tgatccgacg acgacttctc catcgtcggg cggcgtcggc
     4681 cctatccgaa cgccctcacg cggcgaccga ccccaggttg gccgctgctg ttgccttcca
     4741 actcgagggg gcggggtcag acgaaagccc ggagtggtac c
//
LOCUS       DBNHXU010000131         3939 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_40813, whole genome shotgun sequence.
ACCESSION   DBNHXU010000131 DBNHXU010000000
VERSION     DBNHXU010000131.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3939)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3939)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3939
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_40813"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="AC161X_03485"
     CDS             <1..717
                     /locus_tag="AC161X_03485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006947363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988641.1"
                     /translation="MDFVRFTGEWVIYYALIALGGGVLVMLTALILQPVGTDMPELVF
                     EWVIPSGAAGAVIVAAWLVESKQRVVENMAPVLTMLFTPLFALMLTVAAVTYALSGLA
                     GVFDRELLGVFDALLVVVLGLVLYAISARDPSLKPGWMDRVQLVTIASALALDVMVLG
                     TMVARIGDLGFTPNRTAALGLNLVLLVNLAGAAWLSLQFLGGDVAFHRLERWQTSYLP
                     VFAIWAAAVVVVLPPLFGFA"
     gene            914..2500
                     /locus_tag="AC161X_03490"
     CDS             914..2500
                     /locus_tag="AC161X_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020389637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HIR4988642.1"
                     /translation="MLRNRKRLTTLLVMMMVASSIWVAPVASATSNRCERRNNNSIHK
                     LLDCVEVDGVREHQKALQRIADRNDGTRASGTPGYDASVKYVVKKMRAAGYIVTVDPF
                     VFDFFDVLAPSVLDQVSPTATSYVEGTDFTTMDYSGSGDVSAPVQAVDVVVPIGDNPA
                     GTSNSGCEPEDFAGFTAGNIALMQRGTCAFADKAANAEAAGAVAAVIFNEGQPSVDGS
                     EDRAALLFGTLGAAGFGIPVLGTTYALGETLVGLAGAAPGLTMKVFTSTLSETRETAN
                     VFAESRRGATDNIVMAGAHLDSVLAGPGINDNGSGSAALLEIAEQMAKVRPKNQVRFA
                     WWGAEENGLLGSIDWVVRQADSGELANVALYLNFDMIGSPNYVRFIYDGDNSDTGTPG
                     DLPAGSGAIEGLFEKYFAKRHLPTEPTIAGDRSDHFAFAVFGVPVGGLFTGAEEIKTA
                     EQAAVFGGRAGEALDPCYHAACDTYKNVSKRALGQMTDAAAYAIFSFAMSTKLVDEEA
                     GAPVAPLRAAAPDLTLKKAS"
     gene            2561..2920
                     /locus_tag="AC161X_03495"
     CDS             2561..2920
                     /locus_tag="AC161X_03495"
                     /inference="COORDINATES: protein motif:HMM:NF021525.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR4988643.1"
                     /translation="MTDEELQEIAVADEVLIETNGEKGPRRTIIWVVVEGGEVFVRSV
                     LGEKGHWYRRAVADPEVNLIVSGEQHGFRAVSASDADSVQRASNGFVAKYRPGRSLDA
                     MVRDEVLATTLRLDPVD"
     gene            complement(2995..3399)
                     /locus_tag="AC161X_03500"
     CDS             complement(2995..3399)
                     /locus_tag="AC161X_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988644.1"
                     /translation="MTLAIQRQPRFNKVLLALGLVAIVAVLIVAMNQAVESTNAANPV
                     VVTTHKTPGLGHRFEMVQDDYLVPQSFLSGRAAMQAELRLQDGYLPPSAVVGASRDAF
                     VSIQDGYLPPAGWVPTPQVVQDPILLGAIGPQ"
     gene            3646..3897
                     /locus_tag="AC161X_03505"
     CDS             3646..3897
                     /locus_tag="AC161X_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4242 domain-containing protein"
                     /protein_id="HIR4988645.1"
                     /translation="MPLFMDVHHIEGGVSEADVAQAHARDLETQAVYGVEYKRYWVDE
                     ANGKIFCLVEADNAELANTVHREAHGLVADEIYSVSEHA"
BASE COUNT          702 a         1240 c         1287 g          710 t
ORIGIN      
        1 atggacttcg tccgcttcac cggggagtgg gtcatctact acgcccttat cgccctcggt
       61 ggcggggttc tggtcatgct gaccgcgttg atcctgcaac cggtcgggac cgacatgccg
      121 gagttggtct tcgagtgggt catcccctct ggtgcggccg gcgcggtgat cgtggccgcg
      181 tggctcgtcg agtccaagca gcgggtcgtg gagaacatgg ctccggtgtt gaccatgctc
      241 ttcactcccc tgttcgcgtt gatgctgacc gtcgctgcgg tcacctatgc gttgtctggg
      301 ttagccggag tgttcgaccg agagctgttg ggggtcttcg acgccctgct ggtggtcgtc
      361 ctcggcctgg tcctctatgc gatttcggcc cgagatcctt ctctcaaacc ggggtggatg
      421 gaccgcgtcc aactcgtcac catcgccagc gccctcgctc tcgacgtgat ggtcctgggc
      481 acgatggtgg cccgcatcgg tgatctcggg ttcaccccga atcgaacggc ggcactcggc
      541 ctcaacctcg tcctgttggt gaacctggcc ggagcagcct ggctctcgct gcagttcctc
      601 gggggcgatg tcgccttcca ccgcctcgag cgatggcaaa cctcctatct cccggtcttc
      661 gccatctggg cggcggccgt cgtcgttgtg ctccccccgc tattcggctt cgcctgacga
      721 gtcggcggat agcggtcaac taatcagttg actcgccggc tcgtccgttc ggactcgaac
      781 tatggccgcg ctgccgatgt gtcgatagga gtccggcctc cccctcgccg cgttgaggta
      841 ggttctcggg cggatcgggg taaggagtgg gagttccggg tcacatggcc catcgcagat
      901 aggaggatca gcaatgctgc gaaacaggaa gcgtctcacg accttgttgg tgatgatgat
      961 ggtcgccagc tcgatttggg tggctccggt cgcctcggcc acatcgaacc gatgtgaacg
     1021 gcgcaacaac aattcgatcc acaaactctt ggattgcgtg gaggttgacg gggtccgcga
     1081 gcaccaaaag gccctgcagc ggatcgccga ccgtaacgac gggacccggg cctctgggac
     1141 gcccggatac gacgcctcgg tcaaatatgt ggtcaagaag atgcgggccg ccggctacat
     1201 cgtgaccgtc gaccccttcg tgtttgactt cttcgacgtg ctggcgccga gtgttctcga
     1261 ccaggtctcc cccaccgcca cctcctacgt ggagggcacc gacttcacga caatggacta
     1321 ttcaggctct ggcgacgtgt cagcgccggt tcaggcagtg gacgtcgtgg tcccgatcgg
     1381 ggacaacccc gcaggcacct ccaacagcgg atgcgagccc gaggactttg ccgggttcac
     1441 ggcaggaaac atcgccttga tgcaacgcgg cacctgcgct ttcgcggaca aggcggctaa
     1501 cgccgaggca gcgggagccg tggcggccgt catcttcaac gagggccagc cttccgtcga
     1561 tggcagcgag gaccgcgccg cactgctctt cggcaccttg ggcgccgcag ggttcgggat
     1621 cccggtgctc ggcaccacct acgccctcgg cgaaacactg gtcgggctgg cgggggccgc
     1681 tccgggcctg acaatgaagg tctttacgtc cactctctcc gaaacacggg agactgccaa
     1741 cgtattcgcc gagagtcgcc ggggagcaac cgacaacatc gtgatggcag gggcccacct
     1801 cgactcggtg cttgcaggcc cggggatcaa cgacaacgga tccgggtcgg cggcccttct
     1861 cgagatcgca gagcagatgg ccaaggtgcg cccgaagaac caggtgcgtt tcgcttggtg
     1921 gggcgccgag gagaacgggc tgcttggctc catcgactgg gttgtgcgtc aggctgactc
     1981 aggggaactg gccaacgtcg ccctctacct caacttcgac atgatcggct cgccgaacta
     2041 cgtccgcttc atctacgacg gtgacaactc cgacaccggg acaccaggag acctgccagc
     2101 gggctcgggg gcaatcgagg gcctgttcga aaagtacttc gccaagcggc acctccccac
     2161 cgagccgacg atcgccggtg accgctctga tcacttcgcc ttcgcagtgt tcggggtccc
     2221 ggtcggcggc cttttcacag gcgccgagga gatcaagacc gcggaacagg ctgccgtctt
     2281 cggaggccgc gccggcgagg cgctcgaccc ctgttaccac gccgcctgcg acacctataa
     2341 gaacgtgtcg aagcgggcac tgggccaaat gactgacgcc gcggcctacg ccatcttcag
     2401 cttcgccatg agcaccaagc tggtcgacga ggaggccgga gcaccggtgg cacctctgcg
     2461 ggccgccgct cccgacctga cgctgaaaaa ggcgtcctaa tcggttcgag ttggggcgtg
     2521 gcccgaggct gcgccccaac tccgaccctg aggtacgtgg atgaccgacg aagaactcca
     2581 agagatcgcc gttgccgacg aggtgctgat cgagaccaac ggcgagaagg ggccgaggcg
     2641 caccatcatc tgggtggtgg tcgagggagg agaggtgttc gtccggtcgg tcctcggcga
     2701 gaagggacac tggtatcggc gggccgtcgc ggatcccgag gtgaacctga tcgtgagcgg
     2761 cgagcagcac ggattccggg cagtttcggc aagcgatgcc gactccgtcc aacgggcctc
     2821 gaacgggttt gttgccaagt accgaccggg ccgaagcctg gacgccatgg tccgagacga
     2881 ggtgctcgcc accacgctgc gcctcgaccc ggtcgactga gccgggccag tcgatgtcgt
     2941 ccgacgcaaa gggcccccgg atcaatccgg gggccttctt gaggtcggga acggttactg
     3001 ggggccgata gccccgagca gtattgggtc ttgcacgacc tgcggcgtcg gcacccaacc
     3061 ggcgggaggg agatacccat cctgaatgga gacgaacgcg tcccgggaag caccgaccac
     3121 cgctgacggc gggagatatc catcctgcag tcgcagttcg gcctgcatcg cggcccgacc
     3181 gctgaggaac gactgcggga ccaggtagtc gtcctggacc atttcgaagc gatgcccgag
     3241 ccccggggtc ttgtgggttg tcaccacgac cggattcgcg gcattcgtgg actcgaccgc
     3301 ctggttcatg gcaacgatga ggactgccac gatggcgacc aacccaagag cgagtagcac
     3361 cttgttgaat cggggctgtc gctgaattgc aagtgtcatt tcctgcctcc tagttcgagg
     3421 cgctcggtaa gcgccgttcc gttaagcaca ttgcgaacgt atggaggagg cggacagggc
     3481 acatcgggag cgatgcccat cttgggctct actggccccc gagttacggg gttggcgcac
     3541 ctcccagagt ccctatggag ggggccgcaa gatagggagt tctccccatg cggctttcag
     3601 gcatctgcca tacgttggca atcgaggcga acaaggagga acctcatgcc actgttcatg
     3661 gacgtgcacc acatcgaggg aggggtatcg gaggccgacg tggcccaggc ccacgcccgg
     3721 gatctggaaa cccaggccgt ctacggcgtc gagtacaagc ggtactgggt ggacgaagcg
     3781 aacggcaaga tcttctgcct ggtcgaggcg gacaacgccg aactggccaa caccgtccac
     3841 cgtgaagccc atggcctggt cgccgacgag atctactccg tcagcgaaca cgcctgagcc
     3901 aagggaaaca tccaatggtc acatttgcct cggagggaa
//
LOCUS       DBNHXU010000132         3209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_834479, whole genome shotgun sequence.
ACCESSION   DBNHXU010000132 DBNHXU010000000
VERSION     DBNHXU010000132.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_834479"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2960
                     /gene="metH"
                     /locus_tag="AC161X_03510"
     CDS             <1..2960
                     /gene="metH"
                     /locus_tag="AC161X_03510"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0071265 - L-methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HIR4988646.1"
                     /translation="AAIEGGRAAMRAAGVDLPVQVQVTIELTGRMLPGTEIGAALTSL
                     GSLHPTVIGLNCATGPAEMYEPLRHLTRHSRLVVSAIPNAGLPYVEDGKMKYDLGPEE
                     LAEHLATFTTEFGVGVVGGCCGTTPEHLAAVVARVRPVTIKERQPVIIPSASSLYSQV
                     PFDQDTSFMIIGERANANGSRAFRDALLADDLEGCIAIGQDQVKEGAHLVDLCVDYVG
                     RDGVADMIRLASAFATDVAAPVVLDSTESPVMEAGLERLGGRSVLNSANLEDGDAEGS
                     RIDRVFKLAAKHGAAVICLLIDEEGQARTLEWKMRVAHRIHDLAVDRYGLESSDLIFD
                     ALTFPLSTGDDDLRRDGIETIEAIRRIKAELPGVRTVLGVSNISFGLNPAARKILNSV
                     FLHEAVDAGLDGAIVHAGKIEPLSKMDPEQRKVCLDLVYDRREEGYDPLEKLLELFAD
                     VRTEQATVEDYNDLPVGERLARRIVDGDQRSIIEDLEIALGEGTKPLDIINEFLLAGM
                     KTVGDLFASGEMQLPFVLKSAETMKKAVAHLEPKMEKSDTGGRGKIVIATVSGDVHDI
                     GKNLVDIILSNNGYEVHNLGIKVGIAEMIKVFEETGADAIGMSGLLVKSTLIMRDNLE
                     ELERRGMGKVPVLLGGAALTRNYVEADLRSRYAGPLYYGKNAFAGLDAMQRIVAKEPA
                     PAPEAGRQWHKAGEEPVEVPARSPHVQTDNEIFKPPFIGSRIVRGVPFDDITPYVNLT
                     ALFRNQWQYRPEPGENDREFKERLRPLLRDQLARARSADLLRPQLVYGYFAANSEGND
                     LVIWDDESRSTELTRFTFPRQPEEPFYCVSDFFRPMGGEVDYAAFHIVTMGHEVSRRT
                     AELFADNKYHDYLLLHGLGVELAESLAEYWHYRIRQEWGFVAEDGPNLIGLFKQQYRG
                     GRYSWGYPACPDLEDNEKVAWLLGADRLGIEVGEETAFQYQPEQTTSALICHHPQAKY
                     FIAKSPKETRDAD"
     gene            2950..>3209
                     /locus_tag="AC161X_03515"
     CDS             2950..>3209
                     /locus_tag="AC161X_03515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988647.1"
                     /translation="MLTSAGRWLRIAEVNPPEFPDLSHRSLAPAWTHVMVTDNVFGRI
                     RVSPYAFSARITHDTPDVEPTVVVSTRDRNILAIESEVRGALG"
BASE COUNT          638 a         1000 c          990 g          581 t
ORIGIN      
        1 aggccgcgat cgagggcgga cgtgccgcga tgcgggcggc cggcgtcgat ctccccgtgc
       61 aagttcaagt cactatcgag ctcaccggcc ggatgcttcc cgggaccgaa atcggggccg
      121 cgctcacatc gcttggctcc ctgcacccga cggtgattgg cctcaactgc gcaaccgggc
      181 ccgctgagat gtacgagcct ttgcgacatc tcaccaggca ctcgaggctg gtcgtgtccg
      241 ccatccccaa cgccggcctg ccgtacgtcg aggacggcaa gatgaaatat gacctcggcc
      301 ctgaagagtt ggccgagcat ctcgccacct tcaccaccga gttcggggtc ggagttgtcg
      361 gaggatgctg cggaactacg cccgagcacc tcgccgccgt agtggcccga gtccgtccgg
      421 tgaccatcaa ggagcgacag ccggtcatca tcccatcggc ctcctcgctc tatagccagg
      481 ttcccttcga tcaggacacc tcgttcatga tcatcggcga gcgcgccaac gccaacggat
      541 ccagggcatt tcgtgatgct ctgcttgccg acgacctcga aggctgtatc gccatcggcc
      601 aggaccaggt gaaggagggg gctcacctgg ttgacctgtg cgttgactac gtaggtcgcg
      661 acggcgtggc cgacatgatc aggctggctt cggcgtttgc cacagatgtc gcagcgccgg
      721 tcgtgctcga ctcgaccgag tcgccggtga tggaagccgg tctcgaacgt ctcggtgggc
      781 gttcggtcct caacagcgcc aatctcgaag atggggatgc tgagggatcc cgcatcgatc
      841 gcgtcttcaa actggccgcc aagcacgggg cagccgtcat ctgcctcctc atcgacgaag
      901 agggtcaggc aaggaccctc gagtggaaga tgcgagtggc tcatcgcatc cacgatctcg
      961 ctgtcgaccg atacgggctg gagtcgagcg acctcatctt cgatgccctg acgtttccac
     1021 tcagcacggg tgacgacgac ctgcgtcgtg acggcatcga aacgatcgaa gcgatcagga
     1081 ggatcaaggc cgagctaccc ggagtccgga ccgtgctcgg cgtctccaat atctccttcg
     1141 ggctcaatcc cgccgcgagg aaaatcctca attcggtctt ccttcatgag gccgtggacg
     1201 cagggctgga cggcgcgatc gtgcacgccg ggaaaatcga gccgttgtcg aaaatggatc
     1261 cggagcagcg caaggtctgc ctcgacctcg tctacgaccg ccgagaggaa ggctatgacc
     1321 cgctcgagaa gctgctcgag ttgttcgccg acgtccggac cgagcaagcg acggtcgagg
     1381 actacaacga cctcccggtg ggggagcgcc tggctcgacg aatcgttgac ggtgaccagc
     1441 gctcgataat cgaggatctc gagatcgcac tcggtgaggg gacaaagccg ctagacatca
     1501 tcaacgagtt cttgctcgcc ggcatgaaga cagttggtga cctatttgcc agcggtgaga
     1561 tgcaacttcc cttcgtgctc aagtcagcgg agaccatgaa gaaggccgtt gcccacctgg
     1621 agccgaagat ggagaagtcg gacaccgggg gccggggcaa gatcgtgatt gccacggtgt
     1681 caggcgacgt gcatgacatc ggcaagaacc tcgtcgacat catcctgagc aacaacgggt
     1741 atgaggtgca caacctcggc atcaaggttg ggatcgcgga gatgatcaag gtgtttgagg
     1801 agaccggagc cgacgcgatc gggatgagcg gcctcttggt caagtcgacc ctcatcatgc
     1861 gcgacaacct cgaagagttg gagcgccggg gcatgggcaa ggtccccgtc ctccttggcg
     1921 gagccgcgct gacccgaaac tatgtcgagg cggacctcag gtctcgctat gccgggcccc
     1981 tctattacgg aaagaatgcc tttgccggtc tcgacgcaat gcaacggatt gttgccaaag
     2041 aaccggcacc ggcgcccgaa gccgggcggc agtggcacaa ggcgggcgag gagccggtgg
     2101 aggtgcccgc tcgctcgcct catgtccaga ccgacaacga aatcttcaag ccaccgttca
     2161 tcggttcgag gatcgtccgt ggcgtgccat tcgacgacat caccccgtat gtcaatctga
     2221 cggccctttt ccgcaaccag tggcagtacc ggccagagcc cggcgagaac gaccgggaat
     2281 tcaaggaacg actgcgaccc ctcctccgtg accagttggc acgggcccgc tcagctgacc
     2341 ttctccgccc ccagctggtc tacggctact ttgccgcgaa ctccgagggc aatgatcttg
     2401 tgatctggga cgacgaaagt cgcagcaccg agctcacccg attcaccttc ccccgccagc
     2461 ccgaggagcc gttctattgc gtctcggact tcttcaggcc gatgggcggt gaagtcgatt
     2521 acgcagcctt tcatatcgtc accatgggcc acgaggtgtc gcgtcgcacc gccgagctgt
     2581 tcgccgacaa caagtatcac gactacctcc tgctccacgg cctcggagtg gagctcgcgg
     2641 agtcgcttgc cgagtattgg cactaccgca tccgccagga atgggggttt gtcgcggagg
     2701 atggcccgaa cctgatcggt ttgttcaaac agcaataccg cggtggccgt tactcatggg
     2761 gctatcccgc ttgccccgac ctggaggaca acgagaaggt ggcgtggctg cttggggccg
     2821 atcggcttgg gatcgaagtc ggggaggaga cggccttcca ataccagcca gagcagacca
     2881 catcggcctt gatctgtcat caccctcagg ccaagtactt catagccaaa agcccgaagg
     2941 agaccagaga tgctgactag cgccggccgt tggttgcgca ttgcggaagt gaatccaccc
     3001 gagttccccg acctgagtca tcgaagcctg gcaccggcct ggactcatgt gatggtcacc
     3061 gacaacgtct tcggacggat cagggtgtcg ccatatgctt tctcggcccg aatcacacac
     3121 gacacaccgg atgtcgagcc gaccgtcgtg gtgtcaactc gcgaccgcaa cattctggcg
     3181 atcgagtccg aggttcgtgg agccctcgg
//
LOCUS       DBNHXU010000133         2718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2025168, whole genome shotgun sequence.
ACCESSION   DBNHXU010000133 DBNHXU010000000
VERSION     DBNHXU010000133.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2718
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2025168"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1880
                     /locus_tag="AC161X_03520"
     CDS             <1..1880
                     /locus_tag="AC161X_03520"
                     /inference="COORDINATES: protein motif:HMM:NF024947.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HIR4988648.1"
                     /translation="TDKPSERQALLRALLDIGLFEKVMQLANARARTAEGRAQAMEES
                     LAKLDVPTPEQVEEARVRLAAIVEARKELPSRVEALAKLGSALESARAAQEETSLSLA
                     QLGSIVAPKDLATIEKDRVEAFARIEALAGSLTVLAVKRDEINLALTEKAPLTQLESW
                     KANMERLDQLAARRTALDLDGLAAQFEEMSAARQQKQGEFEAARMGHAAHAVRQGLAV
                     GEPCPVCEVVVAKLPPGGDGHGESIDRLSREVSDLESRLGEARDRLKGAEGEAKGVDT
                     QLTEVGALLADAPAAEVVETSIADVRAQLEKRAMLDGEMSAAREAIAEAQARVDELAI
                     RASGLLEALHTARDLVAAKKPPIPGDDPIQAWQIFEAWRLEAAEKAKADLAERGEMVE
                     AAEAGVARASEEMSSWLEGLGIETAGSPEAQLVLAEERRKSEIQELEKSTTEAAEIGH
                     QLETEVTRARVATSLGTHLKSNNFEAWLLEEAMETLVDGANVLLHELTGGAYSLLASR
                     SQFEVVDHRNADLRRTTRGLSGGETFLVALSLSLSMAEQLAELTGTSSRLECVLLDEG
                     FGSLDQESIDIVATVLDELASGGRTVGIVTHVPELSERIPVRFEVSKGAETSTVERVA
                     S"
     gene            1877..>2718
                     /locus_tag="AC161X_03525"
     CDS             1877..>2718
                     /locus_tag="AC161X_03525"
                     /inference="COORDINATES: protein motif:HMM:NF020933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA double-strand break repair nuclease NurA"
                     /protein_id="HIR4988649.1"
                     /translation="MKFTVEAWDPDYGAPSDADLADSTDKVDPSVEIPPDQWRPLLPE
                     CAPAEDILFVDGVRRVDANLWIEREALFPGFGLAATFAAGAVRCNGGAEVVASSVRRG
                     IFTSGPADSIETKVGRYEVMSTKGSTPEELWLGIQERMGDLEGEVAGDHADAALVVVD
                     GPLSHRRHLEGAVGYIKTQHVQYLPDDLRATLGTLPVGHRTPLFLTTTSWSRFSWYLR
                     LANGAGPAGGLVRCEVKADMTVADAARLAHLVSASLPRYASARHKDPRAPQNLYPIGG
                     LEREL"
BASE COUNT          509 a          825 c          927 g          457 t
ORIGIN      
        1 tgaccgacaa gccgagtgag cgccaggcct tgctccgggc gctgctcgac atcggcttgt
       61 tcgagaaggt gatgcagctg gccaatgcaa gagcgaggac tgccgagggt cgagcccagg
      121 cgatggagga gagtctggca aagctcgacg ttccaacccc tgagcaggtg gaggaggctc
      181 gggtgcgcct cgcggcgata gtcgaggccc gaaaagagct cccatcgcgg gtcgaagccc
      241 tggccaagct tggttcggca ttggaatcag ccagggcggc acaagaggaa accagcctga
      301 gccttgctca actgggatcg atagtcgcac ccaaggatct ggcgacgatc gagaaggatc
      361 gagtcgaagc ttttgcccgc atcgaggcat tggccgggtc actgaccgtc ctggcagtga
      421 aacgtgacga gatcaatctt gccctaacgg agaaggcccc tcttacccaa ctcgaatcgt
      481 ggaaggcgaa catggagcgg ctcgaccaac tggcagcgcg tcgcaccgcc ctcgacctcg
      541 acgggctggc cgcccaattc gaagagatgt ccgcagcccg ccaacagaag cagggtgagt
      601 tcgaggcggc aaggatggga catgccgccc acgctgtcag acagggtctc gcagtcggcg
      661 agccatgccc ggtgtgcgag gtggtagtcg cgaagctccc accgggcggc gacggccacg
      721 gcgagtcaat tgatcgcctc tcccgggagg tctccgatct cgagtctcgg ttgggcgagg
      781 cccgggaccg gctgaagggt gccgaaggcg aggccaaagg cgtcgacacc caattgaccg
      841 aggtcggcgc cttgctcgcc gatgctccag cggcggaagt ggtcgagacg tcgattgcgg
      901 acgtgagagc ccagcttgag aagcgagcca tgctcgatgg cgaaatgtcc gctgcgcggg
      961 aagccatcgc cgaggcacag gccagggttg acgagttggc gatcagggct tctggtctcc
     1021 tcgaggctct ccacacggcc cgcgacctgg ttgccgctaa gaagccgcca atcccgggcg
     1081 acgacccgat tcaagcctgg cagattttcg aagcctggcg cctcgaggca gcggagaagg
     1141 ccaaggctga tctggcggag cgcggcgaga tggtcgaggc agcagaagct ggagtggctc
     1201 gggcttcgga agagatgtct tcctggctgg aggggctggg catagaaacg gcaggctcac
     1261 ccgaggcgca gttggtcctt gccgaggagc ggcgcaagtc cgagatccag gaattggaga
     1321 agtcgacgac cgaggccgcg gaaatcgggc atcagctcga gaccgaggtc acccgggctc
     1381 gggtcgccac ttctctcggc actcatctga agtcgaataa cttcgaggcc tggctgctcg
     1441 aggaggcgat ggagaccctc gtcgacgggg cgaacgttct cctgcatgag ctcacgggcg
     1501 gcgcctactc gctgttggcc agccgcagcc agttcgaagt tgtcgaccat cgaaacgccg
     1561 atctgagaag gacaacccgc gggctgtcag gtggcgagac gttcctcgtt gcgttgtcac
     1621 tgtcgctctc gatggcggaa cagcttgcag agctcaccgg cacctcgtcc cggctcgaat
     1681 gtgtgctcct cgatgagggg ttcggctcac tcgaccagga gtcgatcgac atcgtggcaa
     1741 cggtcctcga tgagttggcc agtggagggc gaaccgttgg catcgtgacc cacgtgccgg
     1801 aactgtccga gcggatcccg gtccgattcg aagtcagcaa gggcgccgag acctcgacgg
     1861 tggagagggt tgcctcgtga agttcaccgt tgaagcctgg gatccggatt acggggcgcc
     1921 gtccgacgcc gacctcgccg actccaccga caaagtcgat ccttcggtcg agatcccgcc
     1981 cgaccaatgg cggcccctgc ttccggagtg cgctcctgcc gaagacatct tgttcgtcga
     2041 cggggtgcgg cgtgtcgacg ccaatctctg gatcgagagg gaggcgctgt ttcccggttt
     2101 cgggctggca gccaccttcg ccgccggtgc tgtcaggtgc aatggcggcg ccgaggtagt
     2161 tgcctccagc gtccgcaggg ggatcttcac ctcgggccct gccgattcga tcgaaaccaa
     2221 agtcggccgc tacgaggtga tgagcaccaa gggctccacc cctgaggagc tctggctggg
     2281 aatccaggag cggatgggcg acctcgaagg ggaggtggcc ggggatcatg ccgatgccgc
     2341 cttggtagtc gtggacgggc cactgtcaca tcgtcgtcac cttgagggcg ccgttggata
     2401 catcaagacg cagcacgttc aatatctacc cgatgacctt cgggccacgt tggggacact
     2461 cccggtcggg catcggacgc cactgtttct caccacgacc tcatggagcc ggttctcctg
     2521 gtatctgagg ctggcgaatg gcgcgggtcc ggcggggggt ctggtccgtt gcgaggtgaa
     2581 ggccgacatg acagtcgccg acgccgcccg actcgcccat ctggtctccg cctctctacc
     2641 ccggtacgcc tcggcccgtc acaaggaccc gagggcgccg cagaacctct atccaatcgg
     2701 aggactggag cgcgagct
//
LOCUS       DBNHXU010000134         1877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2225313, whole genome shotgun sequence.
ACCESSION   DBNHXU010000134 DBNHXU010000000
VERSION     DBNHXU010000134.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1877
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2225313"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            111..581
                     /locus_tag="AC161X_03530"
     CDS             111..581
                     /locus_tag="AC161X_03530"
                     /EC_number="3.5.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004943135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HIR4988650.1"
                     /translation="MPQPISPLDYEPAMRAALEAAAAAGAAGDVPVGAVLLDGDGAIV
                     EFDHNRREELSDPTAHAEMLVLSRRARELGDWRLDGYTLVVTLEPCPMCAGAAVLARL
                     GRIVYGTADPKAGAAWSLYNIPQDRRLNHRIELVDGVLAAECAQILEDFFAARR"
     gene            complement(583..1743)
                     /locus_tag="AC161X_03535"
     CDS             complement(583..1743)
                     /locus_tag="AC161X_03535"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4988651.1"
                     /translation="MPTNRYGPNFGKLFAAALLQEISFVLLVHFPGYLDSLGVTESLI
                     GILYAGAAFLGLSARPLLGRILDLTHRRTVLLVTGLFNALVVLLLATTTVWGPWLWGL
                     FLAQRVLQIALFTTILTYAADSIPIEHRTQGLALYGLSGLLPIAVGGVAGDLVIDAFG
                     FPALFVLSATAGVISWLLVWRLPVLPVLGRRPRRGFWHSLVQRNLLPIWWVTLFFSIG
                     LETVFTFTRTYVDERQIGSTGLFFGVYGVTAATMRILGGAHYDRVPQRLFIVGALVSY
                     GLSLTMLALAGSVPLLIVAAVFAGLAHGAIFPILTSQVVGRARTSERGSAMAIFTSIF
                     DIALLLAAPSVGLLIDGFNYKVAFACVAIALGLGATVYAIWDRRIVAQAALV"
BASE COUNT          420 a          590 c          599 g          268 t
ORIGIN      
        1 ggagcgtaaa cccccaacgg catttccagt gccgcgcact agaccagact atgcgatccc
       61 tcctggatgc cagccggaac cggcaggcag gaaaaggata atccgcaacc gtgccccaac
      121 ccatatcgcc cctcgattac gaacctgcaa tgcgagccgc cctcgaggcg gctgccgcgg
      181 ctggagcggc cggggatgtt cccgtgggcg ccgtcctcct cgacggcgat ggcgcaatcg
      241 tggagttcga tcacaaccga cgtgaggagc tctccgatcc gactgcgcac gccgaaatgc
      301 tggtgctctc gcgtcgagcc cgagaactcg gcgattggcg gctcgatgga tacaccctgg
      361 tggtcaccct cgaaccctgc ccgatgtgcg ccggagccgc cgtgttggcc cgcctcggtc
      421 gaatcgtcta cggcaccgca gatcccaagg cgggagcggc ctggtccctc tacaacatcc
      481 cacaggatcg gcgactgaac caccgcatcg aactggtcga tggcgtgctg gccgcagagt
      541 gcgctcagat cctcgaggac ttcttcgccg cccggcgctg actcagacca gggcggcctg
      601 agcgactatt cgccgatccc atatcgcgta tacggtggct cccagcccga gggcgatcgc
      661 aacgcaggcg aaggcgacct tgtagttgaa gccgtcgatg aggaggccga ccgaaggggc
      721 ggccaacaaa agcgcgatgt cgaaaatcga ggtgaagatc gccatcgccg atcctcgctc
      781 cgaggttcga gcgcgaccga cgacctgact ggtcagaatc gggaagatcg ccccatgggc
      841 caacccggcg aagaccgcgg cgacaatcaa caatggcact gagcccgcga gcgcgagcat
      901 ggtgaggctc agcccatacg agacgagagc acccacgatg aagagtctct gtggaacccg
      961 gtcgtaatgg gcgcctccca gaatccgcat cgtggccgcg gtcacgccat acacaccgaa
     1021 gaagagccct gtcgatccga tctggcgctc gtccacatag gtccgggtga aagtgaacac
     1081 ggtctcgagg ccgatgctga agaacaaggt gacccaccag ataggaagca ggttgcgttg
     1141 gaccagcgag tgccagaagc cacgccgtgg tcgtcgaccc agaaccggga gaaccgggag
     1201 ccgccatacc aggagccagg aaatgacacc ggcggtggct gacagaacga acagggccgg
     1261 gaagccaaag gcgtcgatca cgaggtcccc ggccactcct ccaaccgcga ttgggagcag
     1321 tccggaaagt ccgtaaagcg ccagcccctg cgtgcggtgc tcgatcggaa tcgagtcggc
     1381 ggcataggtg agaatcgtcg tgaacagcgc gatttggaga acacgctggg cgaggaacag
     1441 gccccagagc cagggacccc aaaccgttgt cgtggcgagc agaaggacca ctaaggcgtt
     1501 gaacagcccc gtcaccaaca gaacggtcct tcggtgggtg aggtcgagaa tccggcccaa
     1561 caagggtcgg gcagagagac cgaggaaagc ggcgccggcg tagaggatcc cgatcaggga
     1621 ctcggtcacg cccagactgt cgagatagcc cggaaaatgg acaagaagaa cgaaggagat
     1681 ctcctgcaac aacgcagccg cgaagagctt tccgaagttc ggaccgtacc gattggtggg
     1741 catcagccgc ccgaaggtaa caggcgccag ctcggcccat gtcgctctac ctgatgggcc
     1801 tcggtagcct ctccgttgat cgtgctaggt ggggcgctag gggtgccctg aacccgaaat
     1861 cctctacaag cgggacc
//
LOCUS       DBNHXU010000135         2155 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3613419, whole genome shotgun sequence.
ACCESSION   DBNHXU010000135 DBNHXU010000000
VERSION     DBNHXU010000135.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2155)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2155)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2155
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3613419"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1237)
                     /gene="menD"
                     /locus_tag="AC161X_03540"
     CDS             complement(<1..1237)
                     /gene="menD"
                     /locus_tag="AC161X_03540"
                     /EC_number="2.2.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070204 -
                     2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-
                     carboxylic-acid synthase activity [Evidence IEA];
                     GO_process: GO:0009234 - menaquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-succinyl-5-enolpyruvyl-6-hydroxy-3-
                     cyclohexene-1-carboxylic-acid synthase"
                     /protein_id="HIR4988652.1"
                     /translation="MSQPNPSTALARAVADEMARQGVSLAVISPGSRSAALAIAFDQH
                     PKITTRVVLDERSAAFWALGHARATGLPTVVVATSGTAVANHFPAVVEADESMVPLIV
                     ISADRPPEMLHVGANQTIDQVGLFGDRVRWFCNLGPAMSGSDDNWYWRSAVSQAAARS
                     MGHGSRPGPVHLNIAFREPTVPVEEDGRSPVDVYPYDIDGKPGGAPWQEHRIARPGAA
                     AVAARPDTRGLIVAGEGDYEPSPLLAIAQRIGWPILATAMSGLRGPEVVTTYHHLLVS
                     GTPASLRPEMVVTVGRTGPSDRLTALTASGIPQISIDRWGAWHDPKRQATSMLQADPV
                     ATLERLASTVTPDSNWSAGWISADQTMRAVLDEAIGVGEQPTGPSVARALSDVGWEML
                     VAASSMPIRDVDAHTVQGGA"
     gene            complement(1234..2151)
                     /locus_tag="AC161X_03545"
     CDS             complement(1234..2151)
                     /locus_tag="AC161X_03545"
                     /EC_number="4.2.1.113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="o-succinylbenzoate synthase"
                     /protein_id="HIR4988653.1"
                     /translation="MPMRHRFRRVDHREGVLIRGPAGWGEFSPFPDYPPEVTTRWLAA
                     ALEAACSALPEPGRAAIPVNVTVPAVAPLVARGLVLESGATTAKVKVGEPGQAPIEDE
                     DRLEAVRDALGPDGKIRVDVNASWDLDTATERLATFSKFGLEYAEQPVATLEEMIELK
                     KRTDVLLAADELVRLRPDPLEVAVSGAADLVVLKVQPLGGVTRALDIAARAGVPVVVS
                     SALETSIGIYTGLLVASLLPELPYACGLGTVALIEGDPTRDPLVSLDGMLEVRRPEPD
                     PELLARWRPERDRAAEMLRKVRSAAEVLT"
BASE COUNT          386 a          726 c          685 g          358 t
ORIGIN      
        1 tcgcgccccc ttgaaccgtg tgagcgtcga catctctgat cggcatcgat gaggctgcca
       61 ccagcatctc ccacccaaca tcggaaaggg cacgggctac cgatggccct gttggctgct
      121 ccccaacccc gatcgcctca tcaaggactg cgcgcatcgt ttgatcggcc gaaatccacc
      181 cggcactcca gttcgagtct ggggttacgg tcgatgccag ccgttccaac gtggccaccg
      241 gatccgcctg cagcatggat gtcgcttgcc tcttagggtc atgccaggca ccccagcgat
      301 cgatgctgat ctgggggatg ccggaggcgg tgagtgccgt gaggcggtca ctggggccgg
      361 tcctccccac cgtgaccacc atctcgggcc gcaacgacgc cggagtgcca ctgacgagca
      421 gatggtggta ggtggtgacc acctccggac cccttagccc ggacatcgcc gtcgccagga
      481 tcggccaacc aatgcgttga gcgattgcga gcagaggcga cggctcataa tcgccctctc
      541 cggccacgat cagcccgcgg gtgtccggtc tagcggccac cgcggcggca ccggggcgag
      601 cgattcgatg ctcctgccag ggtgcccctc ccggcttgcc gtcgatgtca tacgggtaca
      661 cgtcgaccgg ggatcggcca tcttcttcga caggcacggt tggctcacgg aaggcgatgt
      721 tgaggtggac cgggcctggc cgcgagccgt ggcccattga tcgggcggcg gcctgagata
      781 cggcgcttcg ccaataccag ttgtcgtcgc tgcctgacat cgctgggcca aggttgcaga
      841 accagcgaac cctgtcaccg aagagcccga cctggtcgat cgtctgattg gccccaacat
      901 ggagcatctc cggtgggcga tcggccgaga tgacaatcag cggcaccatc gactcgtcgg
      961 cctcgaccac cgcggggaaa tggttggcaa ccgcggttcc cgaggtcgcc accacgacgg
     1021 tgggcagtcc cgtggcgcgg gcgtggccca gcgcccagaa cgccgccgac cgctcgtcga
     1081 gtaccacccg ggtggtgatt ttcgggtgct gatcgaaggc gatagccaga gccgccgacc
     1141 gcgagccagg cgaaatgacc gcaagggaaa ccccttgcct cgccatctca tcggccaccg
     1201 cccgtgcaag tgcggtgctg ggattgggct ggctcatgtc agcacctctg ccgccgaacg
     1261 gacctttcgc aacatctcag cggcacgatc gcgttccggg cgccatctgg caagaagctc
     1321 aggatcaggc tcaggcctcc ggacctcgag catcccgtcc agggaaacca acggatcgcg
     1381 ggtcggatcc ccttcaatca aggcgacggt ccccagaccg cacgcgtaag gaagctcggg
     1441 caacaacgag gcgacgagga gaccggtgta tatccctatc gaggtctcca aggcggagga
     1501 caccacaacc ggcaccccgg ctcgcgccgc gatgtcgagg gccctcgtaa caccgcccaa
     1561 cggctgcact ttgaggacca cgaggtcggc tgcaccggaa actgcgactt ccaatggatc
     1621 ggggcgaagc cgaaccagct cgtcggccgc caggaggacg tcggttcgct ttttcagctc
     1681 gatcatctcc tcgagagtcg ccaccggctg ttcggcgtac tcgagcccga atttggagaa
     1741 ggtggccaga cgctcggtgg cggtgtcgag atcccagctg gcattgacgt cgacccggat
     1801 cttgccatcg gggcctaacg catctctgac cgcctcgagt cgatcctcgt cctcaattgg
     1861 cgcctgaccg ggctcaccga ctttcacctt cgcggtggtc gcgccggatt cgagaaccag
     1921 acctctcgcc acaagtggtg caacggcagg gactgtgaca ttcacgggga tcgccgcccg
     1981 tcccggctcg ggcaacgcgc tgcaggcggc ctcgagcgca gctgccagcc atctggtggt
     2041 gacttccggg gggtaatcag gaaacggcga aaactcgccc catccggccg ggccccggat
     2101 cagaactcct tcgcggtgat ctacccgtcg gaagcggtgg cgcatcggta tgcgg
//
LOCUS       DBNHXU010000136         1926 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1233690, whole genome shotgun sequence.
ACCESSION   DBNHXU010000136 DBNHXU010000000
VERSION     DBNHXU010000136.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1926)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1926
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1233690"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>1926
                     /gene="carB"
                     /locus_tag="AC161X_03550"
     CDS             <1..>1926
                     /gene="carB"
                     /locus_tag="AC161X_03550"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR4988654.1"
                     /translation="ALLAVGMTLDEISNDITGATPASFEPALDYVVVKVPRFDFAKFP
                     SVPTRLGTSMRSVGEAMAIGRTFPEALQKALRSLENGRAGLNADPAEETVITVPDDEL
                     ATMVSTPTPTRIFALGEAIRRGWSVDEVARLSNVDPWFVDQIAEIHEVRAEIELSHLR
                     DTAVLRNAKRHGFSDRQLGHLWGKPEAEVRAARMAAGLRRTYKTVDTCAAEFEAVTPY
                     LYGTFEDESEVPPAEKPRVVVLGSGPNRIGQGIEFDYCCVHASFALRDAGYETVMINC
                     NPETVSTDYDTSDRLYFEPLTFEDVMEVIEAETPVAVVVQLGGQTPLNLAHALFEAGV
                     PIAGTSPASIALAEDREQFSELCRSLGLKQPPAGSASSVTEAVDVVDQVGLPVMVRPS
                     FVLGGRRMKVIYSIEELADYVRELYGEADPELTGAPILIDRFLEAAVEIDVDAVYDGS
                     ELLLGGVMEHVEEAGVHSGDSDCVTPPPTISDEAHKEILTATRELASALEVRGLINLQ
                     FAVKGDEVYLLEANPRGSRTVPFISKATAVPLARIAARVMMGSSIEELRREGLVPPPS
                     ARPDFVAVKNAVLPWDRFPEEDPLLGPEMKATGEVMGIGPDVGVAFAKALLGAGHVLP
                     SRGRVFLSFADKDKPMG"
BASE COUNT          347 a          602 c          619 g          358 t
ORIGIN      
        1 cggcgctgct ggccgttggc atgaccctcg atgagatctc aaacgacatc accggcgcca
       61 ctccggcgtc cttcgagcct gccctcgact acgtggtggt caaggtgcct cggttcgact
      121 tcgccaagtt cccctcggtc cctacccgtc tcggcacctc gatgcgcagt gtcggcgagg
      181 caatggctat tggacggacc ttccccgagg cgctgcaaaa agccttgcgc agcctggaaa
      241 acggccgggc cggcctcaat gccgatcccg ccgaagagac cgttatcacg gtcccggacg
      301 acgagctcgc cacgatggtc tcgacgccca ccccgacacg gatctttgct cttggcgagg
      361 caattcggag aggctggtcg gttgacgagg tcgcccgctt gagcaacgtc gatccgtggt
      421 tcgtggacca gatagctgag atccatgagg tgcgtgccga aatcgagctc tcacatctcc
      481 gcgacacggc ggtcctgcgc aacgcaaagc gacacgggtt ctccgaccgg caactcggac
      541 atctctgggg caagccagag gccgaagtgc gagcagcgcg gatggcggcc ggattgcgtc
      601 gcacctacaa gacggtggat acctgcgccg ccgaattcga ggcggtcact ccatacctct
      661 acgggacatt cgaagacgag tccgaggtcc ccccggcgga aaagccgcgg gtcgtcgttc
      721 tcggttccgg tcccaaccgg atcgggcagg gcatcgagtt cgactactgc tgtgttcacg
      781 cctcattcgc cctccgcgat gccggttatg agacggtgat gatcaattgc aacccggaga
      841 ccgtgtccac tgactacgac acgtcagacc ggttgtattt cgagccgttg actttcgagg
      901 acgtgatgga ggtcatcgag gctgagacgc cggtcgcagt tgtggtccaa ctcggcgggc
      961 agacaccatt gaatctggcg catgcactct tcgaggcagg ggttccgatc gcgggcacct
     1021 ccccggcttc gattgcactt gccgaggatc gcgagcagtt ctccgagttg tgtcggagcc
     1081 tcgggctgaa acaaccgccg gcgggatcgg cgagctcggt gaccgaggcc gtcgatgtgg
     1141 tggatcaggt cgggttgccg gtgatggtgc gcccttcctt cgtccttggc ggtcggagga
     1201 tgaaggtcat ctacagcatc gaagagctcg ccgactatgt gcgagagctc tatggcgaag
     1261 ccgatccaga gctgaccggt gccccgatcc tgatcgatcg ctttctcgag gccgcggtcg
     1321 agatcgacgt cgatgccgtc tacgacggca gcgagttgct acttggcggg gtcatggaac
     1381 atgtcgaaga agcaggggtc cattctggcg actcggactg cgtcaccccg cccccaacca
     1441 tctccgacga agcacacaag gagattctca ccgcaacccg cgaactggcg tctgctctcg
     1501 aggtgagagg cctgatcaac ctccagttcg cggtcaaggg agacgaggtc tacctcctcg
     1561 aggcgaatcc gagagggagt aggacggtgc cgttcatctc gaaggcgacg gccgtcccgt
     1621 tggcccggat cgccgccagg gtgatgatgg gctcctcgat cgaagagctt cgtcgagagg
     1681 gcctggtgcc ccctccctcc gcccgacccg acttcgtggc ggtcaagaac gcggtcctgc
     1741 cctgggaccg gttccccgag gaggacccgc tcctcggacc ggagatgaaa gcgaccgggg
     1801 aagtcatggg aatcgggccc gatgttggtg ttgccttcgc caaggcgttg ctgggggcag
     1861 gacacgtgct gccttcccgg ggacgggtct ttctctcctt cgccgacaag gacaagccca
     1921 tgggat
//
LOCUS       DBNHXU010000137         7610 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2227006, whole genome shotgun sequence.
ACCESSION   DBNHXU010000137 DBNHXU010000000
VERSION     DBNHXU010000137.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7610)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7610)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7610
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2227006"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="AC161X_03555"
     CDS             complement(<1..1385)
                     /locus_tag="AC161X_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017680343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR4988655.1"
                     /translation="MSFGLFASLRGYDRKWLTGDLIAGLTVWAVLVPEALAYATIAGV
                     SPVIGLYAAPGALLLYAAFGSSKHLVTGPMAATAALSAAAVADLATEPDEILAFTVIL
                     ALVVGVMAVLAGLFRLGFVANFISEPVLKGFIIGLALTIIVGQLPKLFGIEKVEGDFF
                     DQLWGWITGLGETDGLTLAIGLSSLAIVFGFRRFLPRIPGSLVAVGFGIAAVAIFDLG
                     ERGVAIVGEIDAGLPSFGLPEGSLSDYLAIAPAAVGVMLVGFAEGLGAGKTYATRNHY
                     DIDPNRELIGLGAANIASGLSSGMVVNGSLSKTAVNGSAGANSQVSGLVVAVLTVITL
                     LFLTGLFSDLPEATLAAIVIAALVELVDIDSLRELYRTYSDELGKIYGVAARPDFIAA
                     LAAMLGVLIFDTLPGLFIGIIVSILLMVYRASQPHIAGLGVVPGTDIYVDRTRRPDAV
                     APEGIAVIRVE"
     gene            complement(1393..3759)
                     /locus_tag="AC161X_03560"
     CDS             complement(1393..3759)
                     /locus_tag="AC161X_03560"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR4988656.1"
                     /translation="MSDQNIAGRENLPIPDPQWVGLTTYDAKDPDTKFPPIKPIRPPA
                     DAPNVLIILIDDVGFGASSAFGGPCNTPNFERLAAAGLKYSRFHTTALCSPTRAALLG
                     GRNHHSVGMGGITEIATSAPGYSSMRPRNKAPIAEMLKLNGYSTAHFGKCHEVPVWET
                     SPMGPFDRWPSPGNGFEHFYGFIGGETNQYAPAIYKDTVPVEPDRTPEEGYHFTEDMT
                     DHAIDWVRQQKALMADKPFFVYYAPGATHAPHHVPAEWSDKYKGKFDNGWDAQRERTF
                     AKQKELGVIPADAELTARPAEIPGWDEMEDDLKPVLARQMEIYAGFMEHTDHHIGRLL
                     DALEEMEILDDTLIYLIIGDNGASAEGTPTGCFNELVVLNGAAGLETTEFMVSKIGEF
                     GTPEAYNHYAVGWAHAMDTPYQWTKQVASHWGGTRNGTIVHWPKGIEAKGEIRNQFHH
                     VIDVVPTILEAAGIPAPLYVNGIQQAPLEGFSMGYSFDDADAAEAHTTQYFEMFVNRG
                     IYHQGWTAVTRHSTPWVMAPLPAIDDDVWELYGPDDWTQSNDIAADNPEMLAHLQRLF
                     LIEAGKYNVLPLDDRRVERFNADLAGRPQLVHGNSQILFGGMGRLTENSVVVMKNKSY
                     AVTAEIVVPEGGASGTIVAMGGAFGGWSLYAKDGRPAHCYNLFGLQQFKIHGEDPIPA
                     GEHQVRVEFAYDGGGLGKGGTYTLFVDGSPVGEGRVDNTVPMLFSGDETTDVGTDSAT
                     PVTDDLTTKEVHFNGRVKWVQIDLGDDADDADHLISPEERYRIAMARQ"
     gene            complement(3810..5519)
                     /locus_tag="AC161X_03565"
     CDS             complement(3810..5519)
                     /locus_tag="AC161X_03565"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR4988657.1"
                     /translation="MIAGASTGPNGLAKYVPAVGWLRAYDRGWLRPDVLAGATAAAVV
                     IPQAMGYATVAGLPVEIGLYTCTFPMLVYALLGGSRRLSFSSTSTIAALTGLALAQVG
                     ASSGSEAMAAMATLTLMVGLTLIVFRLVRLGWLIEAVSEATTAGLRLGIGLTIVADQL
                     PKVLGIPPADGGFFADVRNVVLHLSDRNGATVLLSLVTLTGLLLMKRWWPRFPGALLA
                     LAGGISLIVFTGAADRGVSTIGNVPTGLPGLMLPSFQHVEALLPFALAIALMSYLESM
                     TAARISRQPEDPPLDNNHEYVASGVAGVVGSLFQTMPVGGGMSQTQVNTNAGARSQLS
                     QLVTVVLAVAVALFLAPVLSNLPEATLGVIVIFSVLGMLQLTQLKRIGAIDPIELVIA
                     GVTAFAALAYDLLVGVIFGVIFTLYFVLRALNHPVVVELRRPAGGGELLPARDGDNAV
                     PGMLALRIEGGLYTLNVRGVQTEILNRATSLDPVPKVVLLDVGGTADTSVTVIDVMAE
                     TDHMLQALGSTLWIANLPTRVEEKVRRTEMWIPWTEAGRVHPTVNSAVAHFEANRPGT
                     PRV"
     gene            complement(5516..6448)
                     /locus_tag="AC161X_03570"
     CDS             complement(5516..6448)
                     /locus_tag="AC161X_03570"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012933004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR4988658.1"
                     /translation="MTNEPLSSWNDTPTRAAIVDFVDKVTDPTTDWFVPEEDRVVVFD
                     NDGTLWSEKPMPIQLDFTIRRFAEMAEEDPSLQDQQPWKAAHEKDFQWFGAAMVKHYH
                     GDDGDLKMLMGAISKAFAALSIGDYSSKVTAFFETADHPTLGRPYRDCGYQPMVELLR
                     YLEASGFTTYIASGGDRDFMRPIAGHIYGIPPERIIGSSFALEYREGEEGTDVLYKSE
                     MEFFDDGPEKPVRIWSRIGRRPVVACGNSNGDIPMMRFANSADLPNLRLLVLHDDAER
                     EFDYTAGAEDALARAADKGWTVISIKDDWSTVFG"
     gene            complement(6450..7118)
                     /locus_tag="AC161X_03575"
     CDS             complement(6450..7118)
                     /locus_tag="AC161X_03575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010985028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAP family protein"
                     /protein_id="HIR4988659.1"
                     /translation="MIGEVLGEAVGVAISPIPIIAVVLMLASARGKSNSLAFLVGWLV
                     GLGLVGVVVLAVADPAGASTDSGPATWVGWLFLVLGAFAVLAGIRMFNKRPRGDDEPP
                     MPKWMAAMDQFTTLKSLGTGFLLAALNPKNLTLTLAASAAIASAGLSSSDEYITLALF
                     VLVGTLGLAIPIGIYFFGGDKAAGTLADLRHWLAINNAAIMSVLFVVIGAKIFGGGLG
                     IVMV"
     gene            7213..>7610
                     /locus_tag="AC161X_03580"
     CDS             7213..>7610
                     /locus_tag="AC161X_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988660.1"
                     /translation="MLVALLIGGCAANTTATSSSITTTTQSLVSSTTITMATTTTTPA
                     TTTTTSAPSTTTTSPGFAHRVSHVTEEDLSASWRPGCPLDVSELRAVEVSHWGFDGEV
                     HTGRLIVATDLAEDVVEIMRDLFDASFPIER"
BASE COUNT         1484 a         2406 c         2374 g         1346 t
ORIGIN      
        1 ctctcaacgc gaatcaccgc aattccctcc ggagcgaccg cgtcgggccg acgggtccta
       61 tcgacgtaga tatcggtccc ggggaccacc cccaggccgg caatgtgagg ctgcgatgcc
      121 cgatacacca tgagcagaat cgaaacgatg atcccgatga agagcccggg cagcgtgtca
      181 aagatgagga caccgagcat cgcggcgaga gcggcgatga agtccggccg ggccgcaacc
      241 ccatagattt tgcccagctc gtcgctgtag gtgcgataga gctccctgag agagtcgatg
      301 tcgaccagtt cgacgagtgc cgcgatcaca atcgcggcca gagtcgcctc gggaaggtcg
      361 gaaaacagcc cggtgaggaa gagcagggtt atcaccgtca ggacagccac caccagaccg
      421 gacacctggc tgttggcccc cgccgacccg ttgactgctg tcttggagag gctgccattg
      481 accaccatcc ccgaggagag cccggaggcg atattggccg ctccgagccc gatcaattcg
      541 cgattgggat cgatgtcgta gtggttacgg gtggcgtagg tcttccccgc ccccagaccc
      601 tcggcgaagc ccaccagcat caccccgacc gcggccggag caatcgccag atagtcggac
      661 agcgacccct cgggaagccc gaaacttggc agcccggcgt cgatctcacc cacaatcgcg
      721 acccccctct cgccgaggtc gaatatggcg accgcagcga tcccgaaacc gaccgccacc
      781 agagagccag ggattctggg taggaagcgg cggaacccga agacgatcgc cagggaggac
      841 agcccgattg cgagcgtgag gccatcggtt tcgccaagac ccgtgatcca gccccaaagc
      901 tgatcgaaaa agtccccctc gaccttctcg atcccgaaca gcttgggaag ctggccgacg
      961 atgatggtga gcgccaggcc gatgatgaac cccttcagca ccggctcgga gatgaagttg
     1021 gcgacgaacc cgaggcggaa caacccagcc agaacggcca tgactccgac caccagggcc
     1081 aggatcaccg tgaaggcgag gatctcgtcc ggttctgtcg ccagatccgc aactgccgcc
     1141 gccgacaatg cagcggtagc ggccatgggc ccggtgacca ggtgcttcga actgccgaag
     1201 gcggcataga ggagtagtgc cccaggtgcg gcatagagac cgatcaccgg ggagaccccg
     1261 gcgatggttg cataggcgag ggcctcaggg accagcaccg cccagaccgt caatccggcg
     1321 atcaggtctc cggtcagcca cttccggtca tacccccgca gcgaggcgaa cagcccgaag
     1381 ctcacggatg atctactgcc gggccattgc gatccggtac cgctcctcag gtgagatcag
     1441 atggtcggcg tcatcggcgt cgtcgccgag gtcgatctgg acccatttca cccgaccgtt
     1501 gaagtggacc tctttcgtgg tcaggtcgtc ggtcaccggg gtcgccgagt cggtgccgac
     1561 atcggtcgtc tcatcgccgg agaacagcat cgggacggtg ttatcgacac ggccctcgcc
     1621 aaccggcgaa ccgtcgacga agagcgtgta ggtgccaccc ttgcccagac caccaccgtc
     1681 gtaggcgaat tcgactcgaa cctggtgctc tccggcaggg atcggatcct cgccatgaat
     1741 cttgaactgc tggagtccaa acaggttgta gcaatgagcc ggtctgccgt ccttggcata
     1801 gagactccat cccccaaaag ccccacccat agcgacgatc gttccactgg ccccgccctc
     1861 ggggaccacg atctcggcgg tgaccgcgta agacttgttc ttcataacca ctaccgagtt
     1921 ctcggtgaga cgccccattc caccaaacag gatctggcta ttgccatgga ccagctgtgg
     1981 ccgcccggcg aggtcggcgt tgaaccgctc gacgcgacga tcatcgaggg ggagcacgtt
     2041 gtatttgcca gcttcgatga ggaacaaacg ttggagatga gccagcatct ccgggttgtc
     2101 tgcagcgatg tcgttcgact gagtccaatc gtcgggcccg tacagctccc agacatcgtc
     2161 gtcaatggcc ggaaggggtg ccatcaccca cggagtcgaa tgtcgcgtga cggcggtcca
     2221 gccttggtgg tagatgcctc ggttgacgaa catttcgaag tattgggtgg tgtgggcctc
     2281 ggcagcgtcg gcgtcgtcaa aggagtaacc catcgagaaa ccttccagag gcgcctgctg
     2341 gattccgttg acatacaacg gcgctgggat cccggccgcc tcgagaatgg tggggaccac
     2401 atcgatgacg tggtggaact ggttgcggat ctcgcccttg gcctcgatcc ccttgggcca
     2461 gtggacgatg gtcccgttcc tggttccccc ccaatgcgat gccacctgct tggtccattg
     2521 gtagggggtg tccatggcat gcgcccaacc caccgcgtag tggttgtagg cctcgggggt
     2581 gccgaactcg ccgatcttgg acaccatgaa ttcggttgtc tccagccctg ccgccccgtt
     2641 gagtaccacg agctcgttga aacagccggt cggggtgcct tcggccgagg cgccgttatc
     2701 accgataatc agataaatca gggtgtcgtc gaggatctcc atctcctcga gggcgtcgag
     2761 aaggcgaccg atgtgatggt cggtgtgctc catgaagcct gcgtagatct ccatctggcg
     2821 ggccagcacc ggcttcagat cgtcctccat ctcgtcccaa cctgggatct cggccgggcg
     2881 ggcggtgagc tcggcgtcgg ccgggatcac gcccaactcc ttctgcttgg cgaaggtgcg
     2941 ctcgcgttgg gcgtcccagc cgttgtcgaa cttgcccttg tatttgtcgc tccactcggc
     3001 tggtacgtga tggggggcat gagtcgcccc tggggcgtag tagacgaaaa acggtttgtc
     3061 ggccattagg gccttctgtt ggcgcaccca gtcaatggcg tggtcggtca tgtcctcggt
     3121 gaagtgatag ccctcctccg gggtgcggtc cggctcgact ggcactgtgt ccttgtagat
     3181 ggccggggcg tactggttgg tctcccctcc gatgaacccg tagaaatgct cgaagccgtt
     3241 gcccgggctc ggccagcgat caaaggggcc catcgggctg gtctcccaca ccgggacttc
     3301 atggcacttg ccgaaatggg cggtcgagta gccattcagc ttgagcatct cggcgatagg
     3361 agccttgttc cggggccgca tcgagctgta gccgggggct gaggtggcga tctcggtgat
     3421 cccacccatt cccaccgagt ggtggttgcg accgccgagg agagcggctc gggtcgggga
     3481 gcaaagggct gtggtgtgga agcgggagta cttgagcccg gccgcggcaa gacgctcgaa
     3541 gttgggagtg ttgcagggtc ccccgaatgc agagctggcc ccgaatccga cgtcgtcaat
     3601 gaggatgatc aggacatttg gggcatccgc gggcggccgg atcggcttga tgggcgggaa
     3661 cttggtatcg gggtctttgg cgtcgtaggt ggtgaggccc acccattgcg ggtcgggaat
     3721 cggcaggttc tcgcggccgg caatgttctg atcgctcatg tggctcctgg agagacgggc
     3781 tcgggaacct acacgtctct cgaggagtgt cagacgcgag gcgtaccagg ccggttcgcc
     3841 tcgaagtggg ccaccgccga attgaccgtg ggatgcaccc ggcccgcctc ggtccatgga
     3901 atccacatct cggtccgtct caccttctct tcgacccgag tcggaagatt ggcgatccac
     3961 aacgtcgacc ccaacgcctg cagcatgtga tcggtctcgg ccatgacatc gatcacggtc
     4021 acagaggtgt cggcagtgcc accaacgtca agcaagacga ccttggggac ggggtccagg
     4081 cttgtcgctc gattcaggat ctcggtctga acaccgcgca cgttgagcgt gtaaagccct
     4141 ccctcgatcc gaagcgccaa catccccgga actgcgttat caccatcacg tgcaggcaac
     4201 agctccccgc cgccagcggg cctcctgagt tccaccacta ccggatggtt gagcgcacgc
     4261 agcacgaagt agagagtgaa gatgactccg aagatcacgc cgaccagcag gtcataggcg
     4321 agcgcggcaa aggcggtgac gccggcgatc accaactcaa ttgggtcgat ggcgccgatc
     4381 ctctttagtt gggtcagctg aagcatcccc agcaccgaga agatcacgat gactccaagg
     4441 gttgcctcag ggaggttgct cagaacaggc gcaaggaata aggccaccgc cacggcaagg
     4501 accacggtga ccagttggct gagttgcgac ctcgcgcccg cgttggtgtt gacctgggtt
     4561 tgagacatcc cgcccccgac gggcatggtt tggaacaagg atccaacgac accggcgacg
     4621 ccggacgcca cgtattcgtg gttgttgtcg agcgggggat cttcgggttg gcgggagata
     4681 cgcgctgcgg tcatcgactc cagataggac atgagggcaa tggcgagggc gaaggggagg
     4741 agcgcctcga catgctggaa cgacggcagc atgagcccgg gcagaccggt gggaacgttg
     4801 ccaatggtcg acaccccccg gtcggcggcc ccagtaaaca cgatgaggct gatcccaccg
     4861 gcaagggcga gcaacgcacc ggggaatctt ggccaccacc gcttcattag cagcagcccg
     4921 gtcagcgtca ccagactaag aaggacggtg gctccattcc tatcagacag gtgcagcacg
     4981 acgtttcgca catcggcgaa gaacccgccg tcggctgggg ggatgcctag cactttggga
     5041 agctggtcgg cgacgatggt gaggccgatc ccgagtctca accccgctgt ggtcgcctcc
     5101 gacaccgcct cgatcagcca gccgaggcga accagccgga agacgatcaa ggtcagaccc
     5161 accatgaggg tcagcgtggc catcgcagcc atcgcttccg agccggaact agctccgacc
     5221 tgcgccaatg caagcccggt cagagcggcg atggtggatg tcgaactgaa actgagcctt
     5281 ctcgagcctc ccagcagggc gtagaccagc atcgggaaag tgcaggtgta gagcccgatc
     5341 tcaacaggaa gcccggcaac ggtggcatag cccatcgcct gagggatgac cacagcggcg
     5401 gctgtggcac cggcgagaac gtcaggacgg agccaccctc ggtcataggc gcggagccag
     5461 ccgacggcgg ggacgtattt ggcgagaccg tttggcccgg tcgacgcccc cgcgatcaac
     5521 cgaacaccgt ggaccagtca tccttgatgc tgatgacggt ccaacccttg tccgcggctc
     5581 gggccaatgc gtcctcggct cctgcggtgt agtcgaactc ccgctccgcg tcgtcatgga
     5641 gaacgaggag gcgcagattc gggagatctg cgctgttggc gaaccgcatc atcgggatgt
     5701 ccccgttcga gtttccgcag gcgaccacgg gtcgacggcc gatccgactc cagatccgca
     5761 ccggcttctc cgggccgtcg tcgaagaact ccatctccga cttgtagaga acgtcggtgc
     5821 cctcctcgcc ctcgcggtac tcgagagcga acgagctgcc gattatccgc tccggcggta
     5881 tcccgtagat atgcccggcg atcgggcgca tgaagtcacg gtcgcccccc gaggcgatgt
     5941 aggtggtgaa gccgctcgcc tccaagtagc gcaacagctc gaccatcggt tgatacccgc
     6001 agtcgcggta cgggcgtccc agtgtcggat ggtcggctgt ctcgaagaag gcggtcacct
     6061 tgctgctgta gtcgccgatg ctgagcgcgg cgaacgcctt cgaaattgct cccatgagca
     6121 tcttgaggtc gccgtcatcg ccgtggtagt gcttgaccat ggccgccccg aaccactgaa
     6181 agtccttttc atgggcggct ttccagggct gctgatcctg caaggaaggg tcctcctctg
     6241 ccatctccgc aaacctgcga attgtgaaat cgagttggat cggcatcggc ttctcggacc
     6301 acagggtccc gtcgttgtcg aacacaacga cccggtcctc ctctgggacg aaccagtcgg
     6361 tcgtagggtc ggtgaccttg tccacgaaat cgacgattgc agcccgggtt ggggtgtcgt
     6421 tccaggatga gagcggttca tttgtcattt caaaccatca caatcccgag gccgccgccg
     6481 aagatcttgg ccccgataac cacgaagagg accgacatga tggccgcatt attgatcgcc
     6541 agccagtgtc tgaggtcggc gagcgtaccg gccgccttgt ctccgccgaa gaagtagatc
     6601 ccgatcggga tcgccagccc caaggttccc accagcacaa acaacgccag ggtgatgtac
     6661 tcatcgctgc tggagagccc cgccgaggca atagccgccg atgcggcgag cgtgagcgtc
     6721 agattcttgg ggttgagggc agccaggagg aacccggttc cgagtgactt cagtgttgtg
     6781 aactggtcca tcgcagccat ccacttcggc atcggtggct cgtcgtcgcc acgtggccgc
     6841 ttgttgaaca tcctgattcc tgcgagcact gcaaacgccc caagcacaag gaacagccag
     6901 ccaacccaag tggcgggccc ggagtcggta ctcgctcccg cggggtcggc cactgcgagc
     6961 accacgacac cgaccagacc caatcccacc agccacccga cgaggaaggc gagtgagttc
     7021 gatttgcccc tggccgaagc cagcatgagg accaccgcga ttatcggtat cgggctgatc
     7081 gccactccca ccgcctcgcc caggacctca ccgatcacag cacccaacag atctcctttg
     7141 cttcttccga gcaaacctag gccaacctgt gacactcgat tcatgcaagg acctggccgg
     7201 tcagcgggac taatgctcgt ggcgctcctg atcggcggct gcgccgccaa cacgacggcg
     7261 acctcgtcat ccatcacaac gacaactcag tccctggtgt cctccaccac gatcacgatg
     7321 gccaccacga ccacgacacc cgccaccacg accaccacgt cggcaccttc gacgacaacc
     7381 acctcgcctg ggttcgctca tcgggtgtcg cacgtgaccg aggaggacct ttcggcgtcc
     7441 tggcgtcctg gatgcccact cgacgtgtcc gaacttcgcg cggtcgaggt gtcccattgg
     7501 ggattcgacg gtgaagttca caccggccgt ctgatcgtgg ccacagatct ggccgaggac
     7561 gttgtggaga tcatgcggga cctgttcgat gcctcgtttc ccatcgagcg
//
LOCUS       DBNHXU010000138         2560 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1829409, whole genome shotgun sequence.
ACCESSION   DBNHXU010000138 DBNHXU010000000
VERSION     DBNHXU010000138.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2560)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2560
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1829409"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            29..529
                     /locus_tag="AC161X_03585"
     CDS             29..529
                     /locus_tag="AC161X_03585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4988661.1"
                     /translation="MEQSFTAEQACRLTNCTHHQLRYWDRVGLVSPSVQRTGGRPGVR
                     RLYSFRDLVALRVVRSLLDNGMSLQRVRRAWDYLRREGEMEDHLADIKLVTDGQTIFH
                     VGSDEGELMDALRQGQLAFFVAIDEIAREVEEDVTRFELDRNRFLDMLRRVEDDVADE
                     QRAASS"
     gene            complement(531..>2560)
                     /locus_tag="AC161X_03590"
     CDS             complement(531..>2560)
                     /locus_tag="AC161X_03590"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF023418.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="transglutaminase TgpA family protein"
                     /protein_id="HIR4988662.1"
                     /translation="HEISQALDLIRFGVAPVFPTSGLVGVLAVLMWAVGALYVWGAST
                     GPTAAMVVPSIALYLQFAVMDRIRAGRGWMAASIAVIALAIAAVALERRTDAGRVRDL
                     HGRPLPRRSRGWAISAALLVATASLFATQGAAGLVPANGNLPWRLGGGYGPGFGGVAF
                     DRLADLQQRIIRRSNAVLFQATVSPDGPPAEQIYWRMESLDNFDGQAWRPSAAAADFY
                     TSDSVIGDPEHAYRGTTEVISERIRIALLRTPVLPTAGTARFLQSDSVNVAGFQTTPD
                     GSVLYQAQMGEGDEYQVEAVFPDYQKDLGALGTLPDGSFSPLFANAAADGAFSEEPAL
                     PPGSLDRPADIERFVDLPEDTPADILRVARVQTRGASTDFERAWLLEHWFRTEFVYST
                     NVSTGAGSLDIDAWLNEPLSENYRTGYCEQFAASMAVLGRAIGIPSRVVWGFTPGKVD
                     VQDDGTEVITVRDNNAHAWVEMWMDGFGWVKFDPTPRGDGIPQSVTTAFDPAPYLPPP
                     DPNAPDIARPGFLDESQIPDRVDQNGNPLNGDGSFLGIASWWWWILPVVALMLLAIPM
                     SKEVRRRRRIHRIREGGDITAVWEEMVDRLADLGQPIPDHQTPLEFANARDRVLVPIA
                     RNYSAAVYGGRNGRATQSDLDHFEQWLKLRYEGPERARAVFSLRSLIDRQP"
BASE COUNT          459 a          793 c          865 g          443 t
ORIGIN      
        1 gtaggtgagt tgttgtagga agggacgagt ggagcagtca ttcacagccg agcaggcatg
       61 ccggctcact aattgcacgc atcatcaact tcgttattgg gaccgggtcg gattggtcag
      121 cccctccgtc caacgaaccg gcggtcggcc gggagtcagg cgcctctact catttcgcga
      181 tctcgtcgca ttgcgcgtcg tgcgcagcct tctcgacaac gggatgtcac tccagcgggt
      241 cagacgggcg tgggactatc tgcgtcggga gggggagatg gaggatcacc tcgccgacat
      301 aaagctggtg accgatggtc agaccatctt ccacgtcggg tctgacgagg gtgagctgat
      361 ggatgccctc cgccagggcc agctggcctt cttcgtcgcc attgacgaga tagccagaga
      421 ggtcgaggag gacgtgaccc ggttcgagct ggatcgcaac cgcttcctcg acatgttgcg
      481 tcgggtcgaa gacgacgtcg ccgacgagca gcgagcggcc agcagttagg tcagggctgg
      541 cggtcgatca acgaccgcag actgaagacg gctctcgccc gctccgggcc ttcgtagcga
      601 agtttcagcc actgctcgaa atggtcgagg tccgattggg tggcccgccc attgcgtccg
      661 ccatagacag cagccgagta gttccgtgcg atcggcacca ggacacgatc tctggcattg
      721 gcgaactcga gcggcgtttg atgatccggt atcggctgtc ccaggtcggc gaggcggtca
      781 accatctcct cccagacggc cgtgatgtcc ccaccctctc tgatccgatg gatccgcctc
      841 cgtcgacgca cctccttgct catggggatc gccagcagca tgagggccac gaccggcagg
      901 atccaccacc accatgatgc gatgccaagg aaggatccgt ctccgttgag ggggttcccg
      961 ttctggtcaa ccctgtccgg gatctggctc tcgtcgagga agccggggcg ggcgatgtcc
     1021 ggggcgttgg gatcgggtgg cgggaggtag ggggcagggt cgaaggccgt ggtgacactt
     1081 tgcggtatcc cgtcaccccg aggggtgggg tcgaacttga cccaaccgaa tccgtccatc
     1141 cacatctcga cccaggcatg ggcattgttg tcccggacgg tgatgacctc ggtgccgtcg
     1201 tcttggacgt cgaccttgcc cggcgtgaat ccccaaacga cacggcttgg gatcccgatt
     1261 gcccggccca atacggccat cgaggcggcg aattgctcgc aatacccggt gcggtagttt
     1321 tcgctcaacg gctcgttgag ccacgcatcg atatcgaggg atccggcgcc ggttgacaca
     1381 ttggtcgaat agacgaactc tgtccggaac cagtgctcca gcagccaggc acgctcgaag
     1441 tcggtgctgg cacctctggt ttgcacccgt gcgacccgga ggatgtctgc cggggtgtcc
     1501 tccggaaggt cgacgaatcg ctcgatgtct gccgggcggt ctaacgagcc gggaggtagt
     1561 gcaggctcct cgctgaaggc tccgtccgcg gctgcgttgg caaatagagg gctgaacgac
     1621 ccgtcgggca gggtccccag cgctcccagg tctttctgat agtcggggaa gaccgcctcg
     1681 acctggtatt cgtcgccctc gcccatctgt gcctgataga ggaccgaccc atccggcgtg
     1741 gtctggaagc cggcgacatt caccgagtct gactgaagga agcgggcggt ccccgcggtc
     1801 ggcaggaccg gggttcgaag gagagcgatc cggatccgct cgctaattac ctcagttgtg
     1861 ccccgatatg catgctccgg atcgccgatg acggagtcgg aggtgtaaaa gtcggcggcc
     1921 gcggcggatg ggcgccaggc ttgcccgtcg aaattgtcga ggctctccat ccgccagtag
     1981 atctgctcgg ccggcggccc atctgggctc acggtggctt gaaataggac agcattggag
     2041 cgcctgatga tcctctgttg gaggtcggcg aggcggtcga aggcgacacc cccgaagcca
     2101 ggcccgtacc cgccgcccag gcgccatggg agattcccat tggcgggcac cagtccggcc
     2161 gccccttgag tggcaaacag ggaagcagtg gcgaccagaa gggcggcgct gatggcccag
     2221 ccacgagaac gccggggcag cggtcggccg tggaggtcgc ggacccgacc ggcgtcggtg
     2281 cgacgctcga gcgccaccgc ggcgattgcg agggcgatca cagcgatcga ggcggccatc
     2341 catccccggc cggcccgaat ccggtccatg accgcgaact gcaggtagag ggcaattgag
     2401 ggtacgacca tggccgccgt cggcccggtg gacgcccccc agacgtagag ggccccgacc
     2461 gcccacatca ggacggcaag gaccccgacc aggcccgagg ttgggaacac gggagccacg
     2521 ccgaacctga tcagatccag cgcctgggag atctcgtgga
//
LOCUS       DBNHXU010000139         2282 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3614966, whole genome shotgun sequence.
ACCESSION   DBNHXU010000139 DBNHXU010000000
VERSION     DBNHXU010000139.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3614966"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..243
                     /locus_tag="AC161X_03595"
     CDS             <1..243
                     /locus_tag="AC161X_03595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988663.1"
                     /translation="VPMTPYFMLVDGAGMVIGEGAASSWRHLLGLLRQSSADGAAIKR
                     TTGGKNDYTEDHLRGAGLHPGDPTLYENPLGEPEST"
     gene            240..698
                     /locus_tag="AC161X_03600"
     CDS             240..698
                     /locus_tag="AC161X_03600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988664.1"
                     /translation="MSRIDRAGVGVEVPTGWEGSISGGGFELMADGAKEPTVMHLASF
                     PLPVERGSFGTGAVELMSGQDVFVSLFEYGPESAGTPLFAAEGIPRPLDARQFDRDAL
                     LHAIPQQSGLQQFFTHNGRAFCLYVVLGSHIDRSELLPQVNAVLESLQID"
     gene            698..1702
                     /locus_tag="AC161X_03605"
     CDS             698..1702
                     /locus_tag="AC161X_03605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4988665.1"
                     /translation="MKRWLASGAVLLVVIGVVVFAWPNAVSAILLQGTFTDDNQSPHQ
                     NGIEAVAAAGITSGCNPPNNTRYCPTRGVTRAEAATFLARALGLPDDGQDYFTDDDGH
                     VLEGGINRVAAAGITSGCNPPVNSRFCPDRTLTRAEFAAFVARGLGLPPTSTNFFIDD
                     NGHVLENSINRIAAAGITVGCNPPTNNRFCPDRILTRGEIATLLTRALSLPHNPQRIL
                     LANWSPISCSKDGKSCSLIVDTFSGRTHLIQEGFFQVLPYQGSEQAQFTSGSTTFTLT
                     LDGTTISTTQLPIASSSTQATRLWNATLVFAGGNHALVGEWRWNGTLVQKTTATIRVD
                     "
     gene            complement(1699..>2282)
                     /locus_tag="AC161X_03610"
     CDS             complement(1699..>2282)
                     /locus_tag="AC161X_03610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004944004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR4988666.1"
                     /translation="RQVARDFALLTVEEGHDVSAFTIIGSGPNSASPHHEPGLRVMQE
                     GDVVVCDFGGRIGGYFSDVTRTFSVGEVGKEETEVHALVEEANRVGRSVVAPGVTCQE
                     VDRWARSVIAGAGYGRFLIHRTGHGIGLEVHEHPYMVEGNDMLMQPGMAFSVEPGIYL
                     PGRFGVRIEDIVVCTETGADVLNKADRGLVVVA"
BASE COUNT          441 a          765 c          668 g          408 t
ORIGIN      
        1 gtgccgatga ccccctattt catgctcgtc gacggggccg ggatggtgat cggcgaagga
       61 gccgcctcga gctggcgcca tttgctcggc ctgctccgcc aatcctccgc cgacggggct
      121 gcgatcaagc ggaccaccgg aggcaagaac gactacacgg aggatcacct ccgcggcgcc
      181 ggcctgcacc cgggtgatcc gaccctctat gagaacccac tcggtgagcc ggagtccaca
      241 tgagccgcat tgatcgcgcc ggagtcgggg ttgaggttcc cacgggatgg gaaggatcga
      301 tctccggagg tggattcgag ctcatggctg acggtgcgaa agaaccgacc gtgatgcacc
      361 tcgcctcctt tccgctccca gtcgaacgag gctcgttcgg caccggggcc gtcgagttga
      421 tgagcggtca ggacgtcttc gtgtctctct tcgaatacgg cccggaaagt gcgggcaccc
      481 cgctctttgc cgccgaaggc attccccggc cacttgacgc tcgacaattc gaccgggatg
      541 cactgcttca cgccatcccc caacagagtg gcctccaaca gttcttcacc cacaacggaa
      601 gggctttctg cctttatgtg gtcctgggat cacacatcga tcgatcagag cttctaccgc
      661 aggtgaacgc ggtcctcgag tcgttgcaga tcgactgatg aagcgctggc tcgcctcggg
      721 cgctgttctc ttggtggtga tcggcgtggt ggtcttcgcc tggccgaatg ctgtcagcgc
      781 catcttgctc cagggaacgt tcaccgacga caaccagagc ccgcaccaga acggaatcga
      841 ggctgtggct gcagccggga tcacctctgg gtgcaatccc ccgaacaaca cccgctactg
      901 cccgacccgg ggtgtgaccc gggcggaggc cgcgaccttc ctcgcccgcg ccctcggcct
      961 gccggatgat gggcaggact atttcacgga cgacgatggg cacgtcctcg aaggcggtat
     1021 caatagggtc gctgctgctg ggatcaccag tgggtgcaac cctccggtta acagcaggtt
     1081 ctgcccggat cgaaccctga ccagggccga gttcgcagct tttgtcgcca gaggcctagg
     1141 acttccgcca acctcgacga atttcttcat cgacgacaac gggcatgtcc tcgagaactc
     1201 gatcaaccgc atcgcggcgg ccgggatcac cgtcggttgc aacccgccga ccaacaaccg
     1261 gttctgcccg gaccggatcc tgacccgagg cgagatcgcc acgctgctca cgcgagcgct
     1321 gagcctaccg cacaacccac agcggatcct tctcgccaac tggagcccga tcagctgcag
     1381 caaggacggc aaatcgtgct ctctgatcgt cgacacattc agtggacgca cccacctcat
     1441 ccaggaagga ttctttcagg tgcttcccta tcagggaagc gaacaggccc aattcacctc
     1501 ggggagcacc actttcacgt tgaccctcga cgggacaacg atctccacaa cgcagctgcc
     1561 gatcgcgtcg tcatcaactc aagccacccg gctctggaat gccacgctcg ttttcgccgg
     1621 cggaaaccac gctctggttg gcgagtggcg ctggaacggc actctggttc agaagaccac
     1681 cgccaccatc cgggtcgact aggcgacgac gacgaggccg cggtcggcct tgttcaggac
     1741 gtccgcgccg gtctcggtac ataccacgat gtcctcgatc ctgaccccga accgacccgg
     1801 cagatagatg ccgggctcga ccgagaaagc catgcccggt tgcatcagca tgtcgttgcc
     1861 ttcgaccatg tagggatgtt cgtgaacctc gaggccgatg ccgtgaccgg ttctatggat
     1921 taagaaacgg ccatagcccg ctcccgctat caccgaacgc gcccagcggt cgacctcttg
     1981 acaggtgacc ccgggagcga cgaccgagcg gccgacgcgg ttggcttctt cgaccaaggc
     2041 gtgcacctcg gtctcctctt tcccgacctc gccgaccgag aaggtccggg tcacgtctga
     2101 gaagtaaccg ccaatccggc caccaaaatc acagaccacc acgtcacctt cctgcatgac
     2161 ccgcagacct ggctcatgat gaggggaggc cgagttcggg cctgaaccga tgatcgtgaa
     2221 ggccgagacg tcgtgacctt cttcgacagt caagagagcg aagtcgcgag ccacctggcg
     2281 ct
//
LOCUS       DBNHXU010000140         8074 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3416866, whole genome shotgun sequence.
ACCESSION   DBNHXU010000140 DBNHXU010000000
VERSION     DBNHXU010000140.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8074)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8074)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8074
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3416866"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..643)
                     /locus_tag="AC161X_03615"
     CDS             complement(83..643)
                     /locus_tag="AC161X_03615"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:NF013468.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HIR4988667.1"
                     /translation="MTIDAAVEAVRAGAVVGLPTDTVYGIGVDPLNGSAVGRLFDLKG
                     RPEHKPIGLLVASIEQARMIGEIEGLAAELATAYWPGALTLVVTPKVILANWVGDSQR
                     STVGIRVPDHPMALELLEACGPLAVTSANHSGGAEASDDIEARGIFGEGVAIYLPGLA
                     PGGEASTVVDATGARLAVLRQGPVRL"
     gene            complement(640..1365)
                     /gene="prmC"
                     /locus_tag="AC161X_03620"
     CDS             complement(640..1365)
                     /gene="prmC"
                     /locus_tag="AC161X_03620"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR03534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HIR4988668.1"
                     /translation="MGLLPEHELRRLQAITSLDRDLDSLIARRLAGEPLQYIEGTAPF
                     ASLDLVVDERVLIPRPETEGLFEIATRMVRTPEVIVDLCTGSGALALALKKTFPGAAV
                     FATDISPDAVEVATVNRFRTGLQVYIAEGDLFDPLPAAIIGTVDLIVANPPYVAEVDF
                     NGLPRDVQKEPRIALVSGSSGLEVIQRIGAVAAQWLRPGGVFVCEIGETQGVSAASSL
                     LDLPGVVRQDLAGRDRYLVAVKP"
     gene            1486..1854
                     /locus_tag="AC161X_03625"
     CDS             1486..1854
                     /locus_tag="AC161X_03625"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR4988669.1"
                     /translation="MSGGKKVLVADDSEAVTTLLVTALEMAGYEVAIAKNGDDAYEMG
                     KTGSYDLALLDHLMPGLLGIEVIARWQADGYEIPVIMLSGVDDERTIVDSLDLGAVDF
                     VRKPFRLPELMARIRQRLRD"
     gene            1907..3049
                     /locus_tag="AC161X_03630"
     CDS             1907..3049
                     /locus_tag="AC161X_03630"
                     /inference="COORDINATES: protein motif:HMM:NF025030.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HIR4988670.1"
                     /translation="MNGSQIALLAVGIVFVVDAVLLLGLIALKSVHRRRMERHDQRRS
                     AYLGVLSRRMSFPLHTDYIDPKVVNDAAFLDAVIDLRNTMAGPEIDTLAGIIDRYGLV
                     KRQAARLRNRFPLGRRLRAAVSLAEMGDESSAPVLIEFLSDREPEIRIQCARGLGRMQ
                     HTPAIDEIIERFGKESPWVRSRFADTLVGFGSGAVWSLLAYIRVNHDHDEDEAVVEAI
                     RVLGTIGDREVGPALTDLLDEFQDSELKIAALDALGSIGGPLAVTPLVGYLNSDDWRL
                     RAKAAHSLGEIGDPVASPALRAGLEDENWWVRRNSAASLAGMMGGLEILHQALHGADV
                     YARDAAAEALADCGELGKARERQEQGQASPADLELLSFMVEDALVP"
     gene            3049..4449
                     /locus_tag="AC161X_03635"
     CDS             3049..4449
                     /locus_tag="AC161X_03635"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR4988671.1"
                     /translation="MVLDVMRWIGYGIISYFILMQTYLIFLGLTSAVAMRRSHHLNRF
                     GRVSDMLSSQTSPPVSIVIPAYNEAMGIIESVRSMSIVGYPRFEIVITNDGSSDETLQ
                     RLIEGFKLERVRIPYRPDIPTAPVRAIYRGRGSVDITVIDKENGGRADALNAGINAAR
                     YPYALCTDADVILDAKCLVAAMRRVVEDRQRTIAVGGNIRPLNGSTVQLGHLIRSEVP
                     KRLVPRMQVLEYVRTFLASRPAWSRMDALPLVSGAFGIWKRSAVIAVGGFTSGHMGED
                     MDLTMRLHRHHIDNKIPYRIVYEPSAVIWTEVPDTFRVLRRQRIRWHRGLMTAVRDFM
                     PMTFNPKYGKLGMVTWAAMFLFEFLAPIIEFAGWILIPLAFFLGALNVTNLLVMLGLA
                     LGVGLMSSLLALLLDESFGYFNGPADTARLVVMAFIENLGFRQVTVAWRIRALIGGKS
                     TRAWGNMERKGVANLG"
     gene            complement(4456..5508)
                     /gene="prfA"
                     /locus_tag="AC161X_03640"
     CDS             complement(4456..5508)
                     /gene="prfA"
                     /locus_tag="AC161X_03640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HIR4988672.1"
                     /translation="MDANVEARLEEIESVFAETEALLSDPEVHADLPRLADLGRKHSE
                     LNGLVTDIRRWRQASTDLAEAEEMSDDPDMAHLAGDLAIEIEVLEDRIRTALVPTDPN
                     DGKDVIVEIRSAAGGDEAAIWAGDLLRMYERFADRHGYRVEAMESSPSEAGGYDKVTV
                     AVKGRGAYSRMKYEAGVHRVQRVPKTESQGRIHTSTATVAVLPEVEEVEVEIDPNDVK
                     IDVYRSTGPGGQSVNTTDSAVRLTHLPTGLVVTCQDEKSQLQNKEKAMRILRARLYQL
                     EMERQHSEVAGARRSQVGSGEWSEKIRTYNYKDNRVTDHRIGLTLRRLDQVLEGDLDE
                     LVDAMAADEQASLLSG"
     gene            complement(5508..6494)
                     /locus_tag="AC161X_03645"
     CDS             complement(5508..6494)
                     /locus_tag="AC161X_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007289547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HIR4988673.1"
                     /translation="MSPNERNYEKAAPIDPAKTVGGQAVIEGVMMRAPGAWSVAVRQP
                     DSTITARREPLARLSERNRAARIPFVRGVLILWESLSLGFRALSWSAEKASGEEEEPL
                     TSSQIGWTMVVAVVLFAGLFIVLPAIAAGFISGDSGILFNVVEGLLRLGLFVGYIWAI
                     GRSAEIGRVFEYHGAEHMSIHAYEAGEPLSVESVRRYPPEHPRCGTSFLLIVVLGSII
                     FFSFFGRPGWAFLIATRLIGIPLIAGVAYELLRWSGTRGGWLASALAAPGIWLQKLTT
                     RVPDESQIEVAISSLVVALDDATLEEVTARGGIPESALAVRRSVNDLSSQEG"
     gene            complement(6537..6803)
                     /gene="rpmE"
                     /locus_tag="AC161X_03650"
     CDS             complement(6537..6803)
                     /gene="rpmE"
                     /locus_tag="AC161X_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HIR4988674.1"
                     /translation="MRTDIHPTYADTHVTCSCGNTFTTKSTKPGEMHVELCNECHPFF
                     TGKQKLVDTGGRVERFQRRYGKTTFTKGSDQPTGDEQAEPVVAE"
     gene            complement(6818..>8074)
                     /gene="rho"
                     /locus_tag="AC161X_03655"
     CDS             complement(6818..>8074)
                     /gene="rho"
                     /locus_tag="AC161X_03655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015657817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008186 - ATP-dependent activity, acting
                     on RNA [Evidence IEA];
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HIR4988675.1"
                     /translation="EEPRPAPRVDGSSEFSDEFRPGGSRQRRRRRGGSGQRDDEFIPE
                     EELEVREGLLDILPEGYGFLRVTGYLPGEKDVYVSANQVRKFRLRKGDIVSGPIRPPR
                     SKEKFPAVVRIDKVNGVTADEAMKRPKFESLTPLFPDQRLRLEIESRPQGILTRIVDL
                     IAPIGKGQRGLIVSPPKAGKTTVLQEIAQSITTNNPECYLMVVLVDERPEEVTDMQRS
                     VKGEVIYSTFDRPAEEHTQVAELALERAKRLVEMGTDVVILLDSVTRLARAYNLAMPA
                     SGRILSGGVDSQALYPPKRFFGAARNIEEGGSLTILGTALVETGSKMDEVIFEEFKGT
                     GNMELRLDRKLADKRIYPAIDIEASGTRKEELLFDQTELTQVWKLRRVLLALDPGAAL
                     ELLIDRLKTTKSNPEFLAEVAKNTGN"
BASE COUNT         1486 a         2553 c         2541 g         1494 t
ORIGIN      
        1 gatcgaccga ctccaggggg tccattgaga tcatgggcgg agggtaccgg tggagtcgag
       61 tcccacgcgg cggccgcccc ggctacagcc ggaccgggcc ctggcgaagg actgcgagac
      121 gggcgccagt ggcgtcaacc accgtcgagg cctcgccacc cggggcgaga ccgggaaggt
      181 agatcgcgac tccctcgccg aaaatgcccc tggcttcgat gtcatccgag gcctcggccc
      241 ctccgctgtg atttgccgag gtgacggcga ggggaccgca cgcctcgagc aactccagag
      301 ccatcggatg atcaggcacc cggatcccaa ccgtgctccg ctgcgaatct cccacccagt
      361 tcgccagaat cactttcggg gtgaccacca gggtcagagc accgggccag taggccgtgg
      421 cgagctcggc ggccagaccc tcgatctcgc caatcatcct cgcctgttcg attgacgcca
      481 ccaggaggcc gatcggcttg tgctcgggcc ttcccttgag gtcgaacagg cggccgacgg
      541 ccgaaccgtt gagcggatcg acaccgattc cataaacggt atcggtcgga aggcccacga
      601 cggcgcctgc ccggaccgcc tcaaccgcgg cgtcgatggt cacggcttga ctgcgacgag
      661 ataccggtcg cgacctgcca ggtcctggcg aaccacaccg gggaggtcca acaacgaact
      721 ggcggcggaa actccttggg tctcccctat ctcacagacg aagacgcccc ccggtcgcag
      781 ccattgggcg gcgaccgccc cgatcctttg aatgacctcc agacccgaag acccggagac
      841 gagggcgatc ctcggctcct tctgcacgtc acgggggagc ccgttgaagt cgacttctgc
      901 aacatatgga gggttggcca cgatcaggtc gacggtgccg atgatggccg cgggtagtgg
      961 gtcgaagaga tctccctcag cgatatagac ctggagcccg gttcgaaacc ggttcacagt
     1021 ggcgacctcg acagcatcag gcgagatgtc cgttgcaaac accgccgccc ccggaaaggt
     1081 cttcttcaga gccagggcga gggcgccgga gcccgtgcac aagtcgacga tcacttccgg
     1141 ggtgcgcacc atgcgggtgg cgatctcgaa aaggccctcg gtttccggcc tcgggatcag
     1201 gacacgttca tccacgacga ggtcgagcga tgcgaacggc gccgtgccct cgatgtattg
     1261 caacggctct cccgccaacc gcctcgcgat gaggctgtcc aggtctcgat cgaggctggt
     1321 gattgcctgg agtcggcgta gctcgtgctc ggggaggagt cccatctcga gcaagagtct
     1381 tacatctgaa atctgattgt ggggatagaa ggcgaatcag gcctggcgtg ggtaccgtca
     1441 cattgcgctt cgtcagaggc gcaccatcta tgacttgaga gattgatgag cggaggcaag
     1501 aaggtcctgg tcgccgatga ctcggaggcg gtcaccacgc ttctcgtcac tgccctcgaa
     1561 atggctggct acgaggtcgc cattgccaag aatggcgacg acgcctacga aatgggaaag
     1621 accggttcct atgacctcgc cctgctcgac cacctcatgc ctggactgct cgggatcgag
     1681 gtgatcgccc gctggcaggc cgacgggtac gagatcccgg tgatcatgct ctccggcgtc
     1741 gatgacgagc gaacaattgt cgacagcctc gatctgggcg cagtcgactt cgttcgcaag
     1801 ccatttcgac tccccgagtt gatggcgagg atccgacaga ggctccgcga ctgaccatcg
     1861 cggtcaactg gccggtcgaa tcgaccgatg gaacctgtga gatctcatga acggaagcca
     1921 gatcgccctg ctcgcggtcg ggatcgtctt cgtcgtggac gcagtgctgc tcttggggct
     1981 gatcgcgttg aagtcggtgc accggcggcg catggaacga catgaccaga ggcggagtgc
     2041 ctacctcggc gtgttgagcc gccgcatgtc gtttcccctt cacaccgact acatcgaccc
     2101 caaagtcgtc aatgacgcgg cgtttctcga cgcggtgatc gacctccgta acaccatggc
     2161 cggcccggag atcgacaccc tggccgggat catcgacaga tacggtttgg tgaagcggca
     2221 ggcggcacgt cttcggaacc gctttccgct gggtcgccgg ctccgggcag cggtgtccct
     2281 cgccgagatg ggggacgagt cttcggcgcc tgtcctcatc gaatttctca gtgaccggga
     2341 gccagagatc cgcattcagt gcgcccgagg gttgggacgg atgcagcaca cgcctgctat
     2401 cgacgagatc atcgaaagat tcggcaagga atcgccttgg gttcggagtc gtttcgccga
     2461 cactctcgtc ggcttcggct ccggtgccgt ctggtcgttg ctcgcctaca tcagggtcaa
     2521 ccacgaccac gatgaggatg aggcggtggt ggaggcgatc cgggtcctag gaaccatcgg
     2581 agatcgtgag gtggggcctg ctctcacgga ccttctcgac gagttccagg attccgagct
     2641 caagatcgcg gcgctcgatg ccctcggctc catcggagga ccgctggccg ttacccctct
     2701 cgttggatat ctcaattcag atgactggag acttcgggcc aaggccgcac attcactggg
     2761 tgagatcggc gatcccgtag ccagccccgc gcttcgcgcc ggtctggagg acgagaactg
     2821 gtgggtccgc cgcaactccg cggcctccct ggccgggatg atgggaggcc tcgaaatcct
     2881 ccaccaggca ttgcacgggg ccgatgtcta cgcccgcgac gctgctgccg aagcgctggc
     2941 cgattgcggc gagctgggca aggcgcggga acggcaagag cagggccagg ccagtccggc
     3001 cgacctagaa ctgctcagct tcatggtcga ggacgcgttg gtgccctgat ggtcctcgac
     3061 gtcatgcgct ggatcggata cgggatcatc tcctacttca tcctgatgca gacctatctg
     3121 atcttcttgg gactgacctc ggccgtagcc atgcggcgta gccatcatct gaatcgattc
     3181 ggccgggttt ccgacatgct gtcgagtcaa acatcgcccc ccgtctcgat cgtgatcccc
     3241 gcctacaacg aagcgatggg catcatcgaa tccgtgcggt cgatgtcgat cgtcggctat
     3301 ccgcggttcg agatcgtgat caccaatgac ggttcgagtg acgagaccct gcaacgcctc
     3361 atcgaggggt tcaagttgga gcgtgtccgc atcccatacc ggcctgacat cccgaccgcc
     3421 ccggtccgag ccatctaccg cggccgcggc tcggtcgaca tcacagtgat cgacaaggag
     3481 aacggtggtc gcgccgacgc cctgaacgcc gggatcaacg ccgcccgcta cccatacgcc
     3541 ctgtgcaccg acgccgatgt gatcctcgac gccaaatgcc tggtggcggc gatgcgtcgg
     3601 gtggtcgagg atcgccagcg aaccatcgcg gtggggggga acatccgccc gctcaacggc
     3661 agcacggtgc aactcgggca cctgatccgc tccgaagtgc ccaagcgcct ggtgccccgg
     3721 atgcaggtcc tcgagtacgt gaggacattc ctcgcctccc gcccggcatg gtcccgcatg
     3781 gacgctctgc cactggtgtc aggcgcgttc gggatctgga agcgatcggc cgtgatcgca
     3841 gtcggtgggt tcaccagcgg gcacatgggc gaggacatgg atctgaccat gcgcctgcat
     3901 cgccatcaca tcgacaacaa gatcccctac cggatcgtct acgagccctc ggcagtgatc
     3961 tggaccgaag tgccggacac gttccgggtt cttcggagac agaggattcg atggcaccga
     4021 gggctgatga ccgcggtgcg agatttcatg ccgatgacct tcaaccccaa atacgggaag
     4081 ctcggaatgg tgacctgggc cgccatgttc ctcttcgagt tcctggcgcc gatcatcgag
     4141 ttcgccggct ggatcctgat cccgctcgcc ttcttcctcg gggcgctgaa tgtgacaaac
     4201 ctcctggtga tgctggggtt ggcgctcgga gtcgggctca tgagctccct cctcgcgctg
     4261 ttgctcgacg agtctttcgg atacttcaac ggccccgccg acaccgcccg cctcgtggtc
     4321 atggcattca tcgagaacct cggattccgc caggtgacgg tggcgtggag gattcgggcc
     4381 ttgattgggg ggaagtcgac cagggcctgg gggaatatgg agcgtaaagg cgtagccaac
     4441 ctcggatagc cgcgtttagc cgctgagcag cgatgcctgc tcgtcggctg ccatcgcatc
     4501 caccaactcg tcgagatcgc cttccaggac ctggtcgagg cggcgcaacg tcagtccgat
     4561 gcggtgatcg gtgacccggt tgtccttgta gttgtaggtc cggatcttct ccgaccattc
     4621 cccactgcct acttgcgagc gccgggcgcc ggccacctcg gagtgctggc gctccatctc
     4681 caactgatag aggcgggccc gaagaatccg catcgccttc tccttgttct ggagttggct
     4741 cttctcgtcc tggcaggtga cgacgagacc ggtggggaga tgcgtgaggc ggacggcgga
     4801 gtcggttgtg ttcaccgatt gcccgccagg gccggtggag cggtagacat cgatcttgac
     4861 gtcattgggg tcgatctcca cctcgacttc ctcgacctcg ggaagcaccg ccacggtggc
     4921 tgtggaggtg tggatccttc cttgggactc ggtcttgggg acacgctgca cccggtgcac
     4981 gccggcttca tacttcatcc gggagtaggc gccccgccct ttgaccgcga cggtgacctt
     5041 gtcatagccg ccggcctcag acggcgacga ttccatggcc tcgactcggt atccatggcg
     5101 atcggcaaat cgctcataca tgcgcaagag gtcgccggcc cagatcgcgg cttcatcacc
     5161 accggcagcg gaccggattt caacaatcac gtccttaccg tcgttcggat cagtgggcac
     5221 cagcgcagtc cgaatccgat cctcgagaac ctcgatctcg atggccaggt ctcccgcgag
     5281 gtgcgccatg tccgggtcat cggacatctc ctcggcttcg gcgagatccg ttgacgcctg
     5341 acgccagcga cggatgtcgg tcaccaggcc attcagctcg gagtgcttcc gccccaggtc
     5401 ggcaagacga ggcagatcgg catggacttc gggatcggac agcagcgctt cggtctccgc
     5461 gaagaccgac tcgatctcct cgagacgggc ttcaacgttg gcgtccatca gccctcctgg
     5521 gaactgaggt cgttgacgga ccgacgcaca gcgagggccg actcggggat ccctcccctg
     5581 gcggtcacct cttcgagcgt cgcatcgtcg agagccacca ccaggctgga gatggcaacc
     5641 tcgatctggg actcgtcggg gactcgagtc gtgagcttct gcagccagat cccgggagcg
     5701 gccagagcgc tggcgagcca tcctccgcga gtgcccgacc aacgaagcag ttcgtaggcc
     5761 acgccggcta tcaacgggat cccgatgagc ctcgtcgcga tcaagaaagc ccatccgggg
     5821 cggccgaaga aggaaaagaa gatgatcgag cccaggacca cgatcagcag gaaactcgtc
     5881 ccacaacggg ggtgctccgg cgggtaacga cggaccgact caacgctcaa cggctccccg
     5941 gcctcgtagg cgtggatcga catgtgctcg gccccgtgat actcgaagac gcggccaatc
     6001 tcggcggatc ggccgatggc ccatatgtag ccgacgaaga ggccgagccg aagcagacct
     6061 tcgaccacgt tgaacaggat cccggagtca cctgagatga agccggcggc gatggccggg
     6121 agcacgatga acaacccggc aaacagcaca acggccacga ccatcgtcca tccgatctga
     6181 gaactggtca gtggctcttc ctcctcccct gacgccttct cggccgacca ggaaagggca
     6241 cggaacccaa gactcaacga ctcccagaga atcagcaccc cacgaacgaa aggaattcga
     6301 gcggcgcggt tgcgctcgga gagtcgggcc aaaggctcac gacgggcggt gatcgttgag
     6361 tcaggctggc gaacggccac cgaccacgcc ccaggagcgc gcatcatcac accttcgatg
     6421 actgcttggc ctccgaccgt cttggccgga tcgatggggg cagccttctc gtaattgcgt
     6481 tcgttagggc tcatatgtga cccccgcgga gcacaatttc gggtgagaag acccgatcac
     6541 tcggcgacga cgggctctgc ctgttcgtcc ccggtcggct gatcggagcc tttggtgaag
     6601 gtcgtcttgc catatcgcct ctggaagcgc tcgacgcggc caccggtgtc gaccagcttc
     6661 tgcttgccag tgaagaatgg atggcactca ttgcagagct cgacgtgcat ctcgcccggc
     6721 ttggtgctct tggttgtgaa agtgttccca cacgagcacg tcacatgggt gtcggcgtag
     6781 gtgggatgaa tgtcggttct caccgctggc tcctgtctca gttcccggtg ttcttggcga
     6841 cctcggcgag gaattcgggg ttgctcttcg tcgtcttcaa acggtcgatg agcagctcga
     6901 gggccgctcc agggtcaagg gcaagcaaga cccggcgcag cttccacacc tgggtcagct
     6961 ccgtttgatc aaacaagagc tcttctttcc ttgttcccga cgcctcgatg tctatggccg
     7021 ggtagatcct cttgtcagcg agcttgcggt cgagacgcaa ctccatgttg ccggtgccct
     7081 tgaactcctc gaagatcacc tcgtccatct tggacccggt ctccacgagg gcagttccga
     7141 gaatcgtcag cgagccgcct tcctcgatgt tcctggccgc cccgaagaat cgcttaggtg
     7201 ggtaaagggc ctgtgagtcg acaccaccgg agaggattcg ccccgaagcc ggcatcgcca
     7261 ggttgtaggc ccgggcgagt cgagtgacgg agtcgaggag aatgactacg tcggtcccca
     7321 tctccacaag tctcttggct cgctccaggg caagctcggc gacctgggtg tgctcttcgg
     7381 cgggacggtc gaaggtcgag tagatgacct cgcctttcac cgatcgttgc atgtcggtga
     7441 cttcttcggg ccgctcatcg acgagcacca ccatcagata acactcaggg ttgttggtag
     7501 tgatcgattg agcgatctcc tgaagcaccg tggtcttgcc tgccttggga ggcgaaacga
     7561 tcagcccacg ctgtcccttg ccgatggggg cgatcaggtc aacgatccgg gtcaggatgc
     7621 cctgtggtcg ggactcgatt tccagccgaa gccgctgatc ggggaagagc ggagtcaatg
     7681 actcgaactt cggccgcttc atcgcctcgt cggcggtgac cccattgacc ttgtcgatgc
     7741 ggacaactgc ggggaacttc tccttggaac gagggggccg aatcggcccg gacacgatgt
     7801 cgcccttgcg gagacggaat ttgcgcacct gattggccga gacatagacg tccttctcgc
     7861 cggggaggta gccggtgacg cgcaggaagc catagccctc aggcaggatg tcgaggagac
     7921 cctcgcggac ctctagctcc tcttcaggga tgaactcgtc atcacgctga ccgctaccgc
     7981 cacgacgacg acggcgttga cggctgccgc cgggacggaa ctcatcggag aactcagaag
     8041 atccgtcgac acgcggggca gggcgcggct cttc
//
LOCUS       DBNHXU010000141         5468 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3019875, whole genome shotgun sequence.
ACCESSION   DBNHXU010000141 DBNHXU010000000
VERSION     DBNHXU010000141.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5468)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5468)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5468
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3019875"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1417)
                     /gene="thrS"
                     /locus_tag="AC161X_03660"
     CDS             complement(<1..1417)
                     /gene="thrS"
                     /locus_tag="AC161X_03660"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HIR4988676.1"
                     /translation="MPIELRYPDGKVVEHADGSTALDVAESIGPRLAKAAVAAKLNGD
                     LVDLSRPILESGDFAVVTLDSPEGLDVLRHSSAHVLAQAVLGLFDESTFAIGPAIEDG
                     FYYDFKVDRPFTPEDLEQIEQRIQEIIAADQPFTRQAVSRDEALEIFSRHQFKKEIIE
                     GVDPGEGAAGEQVTLYRNLDFVDLCRGPHLPSTGRIPAIKLLRSSGAYWRGDENREQL
                     QRIYGTSWPSTAQLEEHLTRLDEAEKRDHRKLGPELDLFSFPTELGSGLSVWHPKGGM
                     LRKTIEDHSRTLHERFGFEFVFTPHLAKADLWKTSGHLDYYDENMYPGMELDNDQEYR
                     VKPMNCPGHVLIYKSKGRSYRELPIRLSELGGVYRYEKSGVVHGLLRARGFTQDDSHT
                     FCTAEQLPSELDLHLDFVLTWLRDFGFTEFEADLSTQPAKSVGEQSRWEVSEKALADS
                     LDQKGVRYRIAEGEGAFYGPKI"
     gene            1484..2341
                     /locus_tag="AC161X_03665"
     CDS             1484..2341
                     /locus_tag="AC161X_03665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HIR4988677.1"
                     /translation="MPATRGNQGMDARELVASLNPSGTVRELSSGANKVFRILEAENQ
                     STIVKVYPVASRERRERHALEALAAAPSVPRIINFGLEGDMAWLKTTDGGHWNLANLP
                     KNLDAVEASGAALRGIHTSGAQITNLAAGIDSDYVLSHYRSTIERLGRYRRRFGMEQV
                     VLDKALELEPPLATVPVPAHTKPVLSKFVIDEEGKVTIVDWEWATLAPSEWDLTLSVW
                     QLARNIGEDAAASFRSGYGAELPESRYRPWVAYHSAMMMLEAAEVRDGRLGSLQYLVD
                     DLTEAVLGT"
     gene            complement(2623..3489)
                     /locus_tag="AC161X_03670"
     CDS             complement(2623..3489)
                     /locus_tag="AC161X_03670"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988678.1"
                     /translation="MPLRRVDTAPDLIEAVVLASQWSESPHQPSLSEATRLQLREGRG
                     TGFVSDDPSGVVTLTATKWPEMAMVEIAVPPSDAASHVWDAAEPAVVSAARERGFRAL
                     ELLTWDTALQSRLREQGWTVARSIKRGSMEAAPLPASGGTYPNVASLLGGETDLDQLI
                     EVYNLAFADHPDAGEWDRAELDKLFSEPWFDRRGLLVDRTAELLTGFCWTKVHPDGVG
                     EIYLLAVRPGHSGRGLGRKLAITGVDYLKVERACPEVIIYWDASNETTDNLYQSIGFS
                     VDRLGEVFQLRL"
     gene            3537..4514
                     /locus_tag="AC161X_03675"
     CDS             3537..4514
                     /locus_tag="AC161X_03675"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013862.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HIR4988679.1"
                     /translation="MNRVGLVLGGGGITGAAYEMAALMTIRLATGWEPNSADVVVGTS
                     GGSFVTALLRNDALGLDSLVLPTDDRDAVTERIRSHVYNRDPGVQVGKWVRHGIVPGI
                     KNPGLTFFMASPAAYHAEGLARWVVTHIGEEKAAGWPDKPTAIVSYDVRSRTRVAFGT
                     ATAPAVGMADAVAASSAIPLLFRPYQIDGRLYVDGGVSSGTHADLVLGSKRPLDLVLV
                     LAPLVADVQRKRARFHEKMFDRAGQRSLAEETRLIKEAWPNCDVVVLSPAPSVQNALR
                     PNPMDASRAVATFTRTLISMKRTLARPEVWELLDHHLGKKVRVRKAVSV"
     gene            4576..4935
                     /locus_tag="AC161X_03680"
     CDS             4576..4935
                     /locus_tag="AC161X_03680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4988680.1"
                     /translation="MPVRPTHFEFYSADPKATVDFLIKAFGWQAQQWGDQEYWLLDTG
                     EGAGINGAVARAGEDGPKTMTTCDVEDLDAAIQAVEISGGMLLTDIIELPGVGRWVEV
                     EGPGGEVFGLMEAIPRP"
     gene            complement(4936..>5468)
                     /locus_tag="AC161X_03685"
     CDS             complement(4936..>5468)
                     /locus_tag="AC161X_03685"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR4988681.1"
                     /translation="WAAETGAFQAHAYDQKDVVAGQGTCGREIIEDLPNADTVIVAVG
                     GGGLIAGVASWIREDARVVAVEPELCPSLYEARRAGEPVEVGIGGIAASSLGTYRIGD
                     HCWAANKWIDDSVLVNDSQIEDAQRWLWNACRMVAEPGAAATIAALRSGAYRPSVGEH
                     VVVVISGANTNPGAVG"
BASE COUNT         1031 a         1732 c         1703 g         1002 t
ORIGIN      
        1 cgatctttgg gccgtaaaag gccccttcgc cttcggcaat tcggtagcgg acgcctttct
       61 ggtcgaggga gtcggcgagc gccttctcac tcacctccca acgtgactgc tcaccaaccg
      121 atttcgccgg ctgggtgctg agatcggcct cgaactcggt gaatccgaag tcgcgcagcc
      181 aggtgagcac gaaatcgagg tggagatcga gctcagacgg cagctgctcg gcagtgcaaa
      241 aggtgtggga gtcgtcctgg gtgaaacccc tggcccgtaa cagaccgtgg acgaccccgg
      301 acttctcgta ccggtagacg cctcccaact cggagagccg gatcggcagc tcccggtagg
      361 accggccctt gctcttgtag atcaggacat ggccggggca gttcatcggc ttgacccggt
      421 actcctggtc attgtccagc tccatgcccg ggtacatgtt ctcgtcgtag tagtcgaggt
      481 gacccgaggt cttccacagg tccgccttgg ccagatgcgg cgtgaacacg aactcgaacc
      541 cgaagcgctc gtggagggtg cggctgtgat cctcgatcgt cttgcgaagc atgcccccct
      601 tcgggtgcca gacgctgagg cccgatccga gctcggttgg gaaactaaac aggtccagct
      661 cgggaccgag tttgcgatgg tctcgcttct cggcctcatc gagacgggtc aggtgctctt
      721 cgagctgggc ggtggagggc cacgatgttc catagatgcg ctgtagctgc tcccggttct
      781 cgtcgccccg ccaatatgcc ccggaacttc tcaggagctt gatggcaggg atccgaccgg
      841 tcgaaggcag atgcgggccc cgacagagat cgacgaaatc gagattgcga tagagggtca
      901 cctgctcgcc ggccgcccct tcgcccgggt cgacaccctc gattatctcc ttcttgaact
      961 gatgccggga gaagatttcg agcgcctcat ccctggaaac cgcctggcgg gtgaacggct
     1021 gatcggcggc aatgatctcc tgaattcgct gctcgatctg ctccagatct tcaggcgtga
     1081 agggtcgatc gaccttgaag tcgtagtaga agccgtcttc gatggcgggg ccgatggcaa
     1141 aggtcgattc gtcgaagagg ccgagcactg cctgcgccag aacatgcgcc gaagagtgtc
     1201 gtaggacgtc gagcccctct ggactgtcga gcgtcaccac cgcgaaatcg ccgctttcaa
     1261 ggatgggccg agacaaatcc accaggtcgc cgttgagctt cgcggccacc gccgccttgg
     1321 ccaatcgagg gccaattgac tccgccacat cgagcgcggt cgacccgtcg gcgtgttcga
     1381 cgaccttccc atccgggtaa cgaagctcta ttggcacggg ttatatctcg ctagcctgaa
     1441 gcgcgccaga ggctaactgc cagccagagc agtctggtcg gtgttgcccg caacgagggg
     1501 aaatcaggga atggatgcac gagagttagt tgcgagtctc aacccgagtg ggactgttcg
     1561 agagctttcg tcaggtgcaa acaaggtttt ccggattctc gaagcggaga atcagagcac
     1621 gatcgtcaag gtctatccgg tcgcctcacg agagcgacgg gaacggcatg ccctcgaagc
     1681 gctggccgca gccccttccg tgccccggat catcaatttc gggctcgaag gcgacatggc
     1741 ctggctcaag acgaccgatg gcggccactg gaatctggcc aatctcccca agaacctcga
     1801 tgccgtcgag gcttcgggtg ctgcattgcg cggaatccac acctcggggg cgcaaatcac
     1861 caatctcgcc gcagggatcg actcggacta cgtgctgagc cattaccgct cgacaatcga
     1921 acgtctcggg cgttatcgga ggcgcttcgg catggagcag gtggtcttgg acaaagctct
     1981 cgagttggaa cctccattgg ccactgtccc tgtcccagct cataccaagc cggtgttatc
     2041 gaagttcgtc atcgacgagg agggcaaagt caccatcgtg gactgggagt gggcaaccct
     2101 ggccccgtcc gaatgggacc tgaccttgtc ggtgtggcag ctggcgcgca acatcgggga
     2161 ggacgctgcc gcctccttcc gttctggcta cggcgccgag cttcccgaga gtcgctatcg
     2221 gccgtgggtc gcctatcact cggcgatgat gatgctcgag gccgccgagg tccgagacgg
     2281 ccggttgggc agcctccagt acctggttga cgacctcacc gaggccgtgc tcggcaccta
     2341 gccgggcgaa atccgcgaaa ttcgccactg cccgtcatag cctctcctgg ctggagcaag
     2401 gaggacggac tggtgttcga agcattggtt gcagcgaccg ctacaacagg tctgttcgct
     2461 ttggctaagg ggatacgcgg aaaccatcag tacagggtcg atcctcatct ggtggtcacg
     2521 ccacgggacg agcgaggcga cgatctgcca tgcccatggt gttatgggcc taccaaagag
     2581 gaggatgccc gctgctcagg gtgcggcaac tccttcggct gatcacaaac ggagctgaaa
     2641 gacctcaccc aaacggtcga cgctgaatcc gatcgattgg tagaggttgt cggtcgtctc
     2701 attggaggcg tcccagtaaa tgatgacctc cgggcaggcg cgttcgacct tcaagtagtc
     2761 gacgccggtg atggccaatt tccgccccaa gccgcgcccg ctgtgcccgg gtcgcaccgc
     2821 aagcagatag atctccccaa caccatcagg atgcaccttg gtccaacaga aacccgtgag
     2881 cagctcggcc gtccggtcta cgagtaagcc cctccgatcg aaccagggct cggagaacag
     2941 tttgtccaac tctgcacggt cccattcccc ggcatccgga tgatcggcaa aggcaaggtt
     3001 gtagacctcg atcagttgat cgaggtcagt ctctcccccc aggagcgaag cgacgttggg
     3061 gtaggtaccg ccggaagcgg ggaggggggc ggcctccatg ctcccccgct tgatcgacct
     3121 cgccacggtc catccctgct cccgcaatcg actctgaagt gccgtatccc aggtcaagag
     3181 ttccagggcc ctaaaccctc tctctcttgc cgccgaaacc acagccggtt ccgccgcatc
     3241 ccagacatgc gaagccgcat cggaaggcgg caccgcaatc tccaccatcg ccatctcagg
     3301 ccacttcgtg gccgtcaggg tgacgacccc cgaaggatcg tccgacacga atcccgttcc
     3361 acgaccctca cgaagttgga gccgggtcgc ttcgctcagc gatggttgat gcggggattc
     3421 agaccattgg gaggcgagga ccacagcttc gatgaggtcg ggcgcggtgt cgacccgacg
     3481 caatggcatt caaggaacgc tactgaaacg gacgatgact gtcctatggt gaccgggtga
     3541 acagggtagg actggtcctc ggagggggag ggatcactgg cgccgcctat gagatggctg
     3601 cgctgatgac gatccgtctc gccaccggct gggagccgaa cagtgctgac gtcgtcgttg
     3661 gcacgtcggg cggctcgttc gtaaccgctc tactccgaaa cgacgccctc gggctcgact
     3721 ccctggtgct acccaccgac gaccgcgacg cggtgacgga gcgcattcgc agtcatgtct
     3781 acaaccggga ccccggcgtg caagttggga agtgggtccg tcacgggatc gttcccggca
     3841 tcaagaaccc tggactgacg ttcttcatgg cctcccccgc cgcatatcac gcagagggac
     3901 tcgctcgttg ggtggtgact cacatcgggg aggaaaaggc cgctggatgg ccagacaagc
     3961 caacggcgat cgtttcctac gacgtgagga gccgcaccag ggttgcgttt ggaaccgcaa
     4021 cggcaccagc agtggggatg gccgatgcgg tggccgcgtc ctccgcgatt cccctcctct
     4081 tccgcccgta ccagatcgat ggccgcctgt atgtggacgg gggcgtgagc tcgggcacac
     4141 acgccgacct ggtcctcggt tcgaagcgcc ccctcgacct ggttctggtg ttggcccccc
     4201 tcgtcgccga tgtccagaga aagcgggcgc ggttccacga gaagatgttc gatcgggcag
     4261 ggcaacgatc actcgccgag gagacccggc tgatcaagga ggcgtggccc aactgcgacg
     4321 tggtggtcct cagccccgca ccgtcagttc agaacgccct acgccccaac ccgatggacg
     4381 cctcgcgggc ggtggccacc tttacccgca ccctgatctc gatgaagcgc accctggccc
     4441 ggcccgaagt gtgggagtta ctcgaccacc acctcggaaa aaaagtgcgt gtgcgaaagg
     4501 ccgtctccgt ctaatcggtt gcgttctcgc ttcttcgttc tacgttctac atgtaaatca
     4561 agaggagaaa caccggtgcc ggtccgcccc acacacttcg agttctattc ggctgatccg
     4621 aaggcgactg tcgacttcct gatcaaggct ttcgggtggc aggcccagca atggggagac
     4681 caggagtact ggctgctcga caccggtgaa ggagccggga tcaacggcgc ggtggcacgg
     4741 gcgggcgagg acggtccgaa gaccatgacc acctgtgacg tcgaagacct cgatgccgcc
     4801 atccaggcgg ttgagatcag cggcggaatg ctgttgacgg acatcatcga gctgccggga
     4861 gtcggccgct gggtcgaagt ggagggccca ggtggagagg tattcggctt gatggaggcc
     4921 attccccgcc cctgatcagc caaccgctcc tgggttggtg ttggcgccgg agatgacgac
     4981 gaccacatgc tcgcccacgg atgggcgata ggcaccggac ctgagggcag caatcgtggc
     5041 cgccgcgccc ggctcagcca ccatccggca tgcgttccac agccatcgct gggcatcctc
     5101 gatctgggag tcgttgacca ggaccgagtc gtcgatccac ttgtttgccg cccaacaatg
     5161 atccccgatc cggtaggtgc ccaaggacga cgccgcgatc cccccgatgc cgacttcgac
     5221 cggctcgccg gcgcggcgtg cttcgtaaag gctcgggcat agctcaggct cgaccgccac
     5281 cactcgggcg tcctctcgga tccagctcgc aacgccggcg atgagcccgc caccgcccac
     5341 cgccacaatc acggtgtcgg cgttcggaag gtcctcgatg atctcccgcc cgcaagtccc
     5401 ctgtccggcg actacgtcct tctggtcgta ggcgtgggct tggaaggccc cggtctccgc
     5461 cgcccacg
//
LOCUS       DBNHXU010000142         2231 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4211086, whole genome shotgun sequence.
ACCESSION   DBNHXU010000142 DBNHXU010000000
VERSION     DBNHXU010000142.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2231)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2231
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4211086"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="AC161X_03690"
     CDS             <1..554
                     /locus_tag="AC161X_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008329248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR4988682.1"
                     /translation="TPGSTSPRQNEEGFTRIVPSERTFSYVLCFEPRIEVTQSDVRAV
                     QLAKAALYAGARLLMDHYGVDHVDEIRLAGAFGSHIDPLYAMVLGMIPDCDLDRVSAA
                     GNAAGVGAMMALLSGVARDEIEATALAIEKVETAVEPRFQEHFVAALAIPHKTDGFPM
                     LSAAIDLPSPTESRGRRGRRSGQ"
     gene            600..2135
                     /locus_tag="AC161X_03695"
     CDS             600..2135
                     /locus_tag="AC161X_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR4988683.1"
                     /translation="MEQTSGRRGGGRAARQAARSARAVDSVPFLTRKLAPVEVLGEEG
                     LSLIESNAEMLLERVGIEVLNYPEALEIFRGAGCEIDETRVRFPAGLARSLLATAPNR
                     FTQHARNPEHDVIIGDPHVVFAPAYGSPFVRDLEGGRRYATIGDFENFARLTHMTSSL
                     HHGGGTLCEPVDLPVNKRHFDMVFAHIKNHDKAFMGSVTAPQRAQDTVDMANIAFGGE
                     VLQGKTVLLSLCNANSPMSWDYNMLGSAKIYAENNQACLMTPFILAGAMAPVTVAGTA
                     TQTLAEAMAGMAFCQIVRPGAPVVFGSFASSISMQSGAPTFGTPEPALVLFVMAALAR
                     RLGIPFRSGGNLTAAKTPDYQAAYESAATFLPTILAGVNFVLHTAGWLEGGLAMGYEK
                     FILDADQAGMAEAILGGIDLSENGQALDALLDNPPGQHFLGNAHTLANFESAFWRSGT
                     ADNNSFEQWQEAGSQEAVSRASAIWRRMLTEYEPPALDEAIEAELVDWIGERKASFPD
                     SDV"
BASE COUNT          408 a          707 c          698 g          418 t
ORIGIN      
        1 cgacccctgg gtcgacatct ccgcgccaaa acgaggaagg gttcacccga atcgtgccct
       61 cggagcgaac cttcagctat gtgctctgct tcgagccccg gattgaggtc acgcagagcg
      121 atgtccgcgc cgtgcaattg gccaaggccg cgctgtacgc cggcgcccgg cttctgatgg
      181 atcactacgg ggtcgaccat gtcgacgaga tcaggttggc cggagcgttc ggcagccata
      241 tcgatcccct ctacgccatg gtcctgggaa tgattccgga ctgcgacctc gaccgggtat
      301 cagcagccgg aaacgcggct ggagtgggcg cgatgatggc cctcctttcg ggggtcgctc
      361 gtgacgagat cgaagcgacg gccctcgcta tcgagaaggt ggaaaccgcg gtggagcccc
      421 ggttccagga gcattttgtt gctgccctgg cgatacccca caaaaccgac gggttcccga
      481 tgctctccgc ggcgatcgac ctgccgtccc cgacggagtc cagggggcgc cggggacgtc
      541 gttccgggca atagtggcct tacaccgtcc tgttccatga tgtagtctgg taccacatca
      601 tggaacagac atcgggacgc cggggtggtg gacgcgccgc ccgacaggcg gcccgctccg
      661 cccgggccgt tgattcggtc ccctttctca ccagaaaact cgcaccggtc gaagtgttgg
      721 gtgaagaggg gctgtcgctg atcgagtcca acgccgagat gcttctcgaa cgcgtcggga
      781 tcgaggtgct caactatccg gaggctctcg agatcttccg cggcgccggc tgtgagatag
      841 acgagacccg cgtccgcttt ccagccgggt tggcccggag tctgctggcg accgctccga
      901 atcgattcac ccaacacgct cggaatccgg agcatgacgt gatcattggt gatccccacg
      961 tggtgttcgc cccggcctac gggtcgccat tcgttcgtga cctggaaggt ggtcgccgct
     1021 acgccaccat cggcgacttc gaaaacttcg cccgactcac tcacatgact tcatcgctgc
     1081 atcacggtgg gggaacgctt tgcgaaccgg tcgacttgcc ggtcaacaag cggcatttcg
     1141 acatggtctt cgcccacatc aagaaccacg acaaggcatt catgggctcg gtgacagccc
     1201 ctcagcgcgc ccaggacacg gtcgacatgg ccaatatcgc attcggtggc gaggtcttgc
     1261 aagggaagac ggtgctgctt tcgttgtgca acgccaacag cccgatgagc tgggactaca
     1321 acatgctcgg ctcggcgaag atctacgcag agaacaacca ggcctgtctg atgacgccgt
     1381 tcatcctcgc cggggcgatg gctccggtga ccgtcgcagg aaccgccact cagaccctgg
     1441 ccgaggcgat ggccgggatg gctttctgtc agatcgtgcg ccccggtgcc ccggtggtct
     1501 tcggatcgtt tgcctcttcg atctcgatgc aatcgggtgc gccgacgttc ggaacacccg
     1561 agccggcgtt ggtgctgttc gtgatggcgg cgctggcgcg gcgcttgggt attccgttcc
     1621 gcagcggcgg aaacctcacc gcggccaaga cacctgatta tcaggccgcc tacgagtcgg
     1681 cggccacctt ccttccgacg atcctggccg gggtcaactt cgtccttcac accgcgggat
     1741 ggctcgaagg cggcctcgca atgggctacg aaaagttcat cctcgacgcc gatcaggccg
     1801 gcatggccga agccatcctt ggcggcatcg atctctccga gaacgggcaa gccctcgatg
     1861 ctcttctcga caacccgcca ggtcagcact tcctgggcaa tgcccacact ttggccaact
     1921 tcgaaagcgc cttctggcgg agcgggaccg ccgacaacaa cagtttcgag caatggcagg
     1981 aagccgggtc acaggaagcc gtgagtcggg cgagcgcgat ctggcgccgc atgctcaccg
     2041 agtatgaacc gccagctctc gatgaggcga tcgaggcgga attggtcgat tggattggcg
     2101 agcgcaaggc gtcattcccg gactccgacg tctgaatcat gagtgagagc ctggtcaagg
     2161 cgcttctcac aggagccgac ttcgaggtga tccggctgaa gtccgtcatg gatcaggtgg
     2221 ccacgattcc t
//
LOCUS       DBNHXU010000143         2983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2425609, whole genome shotgun sequence.
ACCESSION   DBNHXU010000143 DBNHXU010000000
VERSION     DBNHXU010000143.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2425609"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /gene="carB"
                     /locus_tag="AC161X_03700"
     CDS             <1..480
                     /gene="carB"
                     /locus_tag="AC161X_03700"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: protein motif:HMM:NF014225.7"
                     /note="four CarB-CarA dimers form the carbamoyl phosphate
                     synthetase holoenzyme that catalyzes the production of
                     carbamoyl phosphate; CarB is responsible for the
                     amidotransferase activity; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HIR4988684.1"
                     /translation="EVMGIGPDVGVAFAKALLGAGHVLPSRGRVFLSFADKDKPMGLA
                     VAQAFTMLGFELLATHGTARYLRHHAIEADAVNKVGEGPRDIGERLENGEVQLVINTP
                     RGGRARSDGRTIRRAARSHAVPCVTTIQGGLAVARSLRAGSVAINRPRSLQEWHQTV"
     gene            462..1379
                     /locus_tag="AC161X_03705"
     CDS             462..1379
                     /locus_tag="AC161X_03705"
                     /inference="COORDINATES: protein motif:HMM:NF013355.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR4988685.1"
                     /translation="MAPDGLSATLGSIQLRTPLIAASGTVGSVWEWAEIADVSAYGAA
                     VAKSVAPVEWPGRPAPRLAPSGPGMLNGIGIQNPGIESWVETMRPILGRLEVPVWGSA
                     VGSTAEEFAIVAKGLVTAGIEVVEVNLSCPNLEDGRMFALDADRSREVIEAVVGSGDV
                     LVGAKLSPNADDIVSVAEACLNGGATFLTLTNTALGFGVSVEDRRPLLSGGVGGYSGP
                     GLKPLSLRCVYEVARALPGTPIVGCGGITTGRDVVEYLLTGASAVGLGTVHFSEPRAG
                     TRIIRELSQEMKRLGVSYIGELVGAVRPW"
     gene            1376..2068
                     /gene="pyrF"
                     /locus_tag="AC161X_03710"
     CDS             1376..2068
                     /gene="pyrF"
                     /locus_tag="AC161X_03710"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011422991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HIR4988686.1"
                     /translation="MNPILVALDFPTGDQALSVAGPLVPLVGGFKVGLELLTGEGPDI
                     IRKVGKLGRPVFADAKLHDIPNTVHGAARQLGKAGARWVTIHGSGGREMIEAAVSGLA
                     DGGDAAGVLVVTVLTSLDEAQLRETGVDRGLESQVVAITQLALDGGAEGIVCAVPEAR
                     AAKDAAPSLQVVTPGIRGADASPDDQKRVASISQALAAGADMVVVGRAITGASDPVTA
                     ARTLAAEAGWSV"
     gene            2123..2434
                     /gene="mihF"
                     /locus_tag="AC161X_03715"
     CDS             2123..2434
                     /gene="mihF"
                     /locus_tag="AC161X_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020520006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor, actinobacterial type"
                     /protein_id="HIR4988687.1"
                     /translation="MQPPQLTDEQRKAALVKAAEARRARAEVKELLKTGSLRLSELVE
                     RAETDDILAGLKVERVIASMPGTGKIKAKRMMETLGIADNRRLRGLGDKQKEALLAEF
                     S"
     gene            2436..>2983
                     /gene="gmk"
                     /locus_tag="AC161X_03720"
     CDS             2436..>2983
                     /gene="gmk"
                     /locus_tag="AC161X_03720"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017326484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - GMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006163 - purine nucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HIR4988688.1"
                     /translation="MSGRLLVVSGPSGSGKSSIVNELLERLDLDFSVSVTTRLPRPGE
                     RHGVHYTFISRKDFEAMIEAGELLEWAQYNNRFYGTPSAPVTAANAAGRDVLLEIEIQ
                     GARQIREERPDAVMFFISPPSIEELEKRLIRRGDTSAADIEDRLEVAETEIAEAPELF
                     DHIVVNDHLDRAVAEIEALITGT"
BASE COUNT          520 a          901 c         1056 g          506 t
ORIGIN      
        1 gaagtcatgg gaatcgggcc cgatgttggt gttgccttcg ccaaggcgtt gctgggggca
       61 ggacacgtgc tgccttcccg gggacgggtc tttctctcct tcgccgacaa ggacaagccc
      121 atgggattgg cggtcgccca ggcgttcacg atgttgggct tcgagctgct cgcaacccat
      181 ggcaccgccc gttacctcag gcatcacgcc atcgaggccg atgctgtcaa caaggtgggt
      241 gaaggaccgc gcgacatcgg ggagcgtctc gagaacggtg aggtccaatt ggtcatcaac
      301 acgccgcgcg gaggtcgcgc caggtcggac gggaggacta tccgccgggc ggcccgcagt
      361 catgccgtgc cctgcgtcac caccattcag ggcggtttgg ccgtcgctcg cagcctgagg
      421 gcgggatcgg tggccatcaa ccggccgagg agccttcagg aatggcacca gacggtctga
      481 gcgccacgct cggctcgatc cagcttcgca cccctttgat agctgcttcg ggaaccgtgg
      541 gctcggtatg ggagtgggcg gagatcgccg acgtgagcgc ctacggcgcg gccgtggcca
      601 agtcggtcgc acccgtcgaa tggccgggtc ggccggcgcc gcgtctggct ccaagtggac
      661 cggggatgct gaacggcatc ggaatccaaa acccgggcat cgagagctgg gttgagacga
      721 tgcgcccgat cctgggacgt ctcgaagtcc cggtgtgggg ctcggcggtg gggtcgaccg
      781 ccgaagagtt cgccatcgtg gcaaaaggcc tggtcacagc gggtatcgag gtggtcgagg
      841 tcaatttgtc gtgtcccaat ctcgaagacg ggcgcatgtt cgcactggat gccgatcgct
      901 cccgggaagt catcgaggcc gtggttggct ccggggacgt cctggttggt gccaagttgt
      961 cgcccaacgc cgacgacatc gtctccgtgg ccgaggcctg cctcaacggg ggtgcgacat
     1021 tcctcactct gaccaacacc gccctcgggt tcggagtctc agtcgaggat cggcgacctt
     1081 tgctctcagg cggtgtcggc ggttacagcg ggcctggtct gaagccgctg tccttgcgat
     1141 gcgtctacga ggtcgcgagg gcgcttccgg gaacgccgat cgtcggctgt ggtgggatca
     1201 cgaccgggcg cgatgtcgtc gagtacctcc tgaccggtgc gtcggcggta ggcctgggca
     1261 cggttcattt ctccgagccc agggccggga cccggatcat ccgcgagctc tcgcaggaaa
     1321 tgaaacggtt gggggtctcc tatatcggag agctggtggg ggcggtgagg ccctggtgaa
     1381 cccaatcctg gtcgcgctcg actttcccac cggagaccag gcgctttccg tggccggccc
     1441 acttgtgcct ttggtcggcg ggttcaaggt gggtctcgaa ctgctgacgg gcgaaggccc
     1501 cgacatcatc cgcaaggtcg gcaagctcgg ccgtcccgtg ttcgccgacg ccaagctgca
     1561 tgacatcccg aacaccgtcc acggagcagc ccggcagttg gggaaggcag gagcgcgttg
     1621 ggtgaccatc cacggttccg ggggccgaga gatgatcgag gccgccgtaa gcggtctcgc
     1681 agacggcggc gatgcggccg gggtgttggt ggtcacagtc ctcacgtcgc tggacgaggc
     1741 ccagttgagg gagaccgggg tggaccgggg gctcgagagc caggtggtag ccatcaccca
     1801 gctcgccctt gatggaggag ccgaaggcat cgtgtgcgcg gtgcccgaag cgcgggctgc
     1861 gaaggatgcg gcgccgtcgt tgcaggtggt cactcccgga atccgcgggg ccgacgcctc
     1921 cccggacgat caaaagcggg tggcctcgat cagccaggcc ctggccgcgg gagccgacat
     1981 ggtggtcgtc gggcgggcga taaccggagc gtcggatccg gtcacggcgg cccgcaccct
     2041 ggcagccgag gccgggtggt cggtttaggc gacttgctcg ccctctccgg ggctatggtc
     2101 agggaccccg acaggagtgg gaatgcagcc acctcaattg actgacgaac agcgaaaagc
     2161 agcgctggta aaggccgcag aagcgagacg ggcgcgcgcg gaagtgaaag agctcctcaa
     2221 gaccgggtcc ctccggctgt ccgagctcgt ggagcgtgcc gagaccgacg acatcctcgc
     2281 cggcctcaag gtggagcggg tcatcgcctc gatgcctggc accgggaaga tcaaagccaa
     2341 gcggatgatg gagacgctgg gcatagccga caatcggcgg cttcggggtc tgggtgacaa
     2401 gcagaaagag gccctcctag ccgagttctc gtgacatgag cgggcggctc ctggtggtgt
     2461 ccgggccttc gggctcgggc aaatcgagca tcgtcaacga gctgctggag aggttggatc
     2521 tcgacttctc ggtctcagtc accacccgcc ttccccgacc gggggagcgg cacggggttc
     2581 actacacctt catcagccgc aaggacttcg aggcaatgat cgaagccgga gaattgctgg
     2641 agtgggccca gtacaacaac cgcttctacg gcaccccctc ggccccggtc acggccgcaa
     2701 atgcggcagg acgggacgta ttgctcgaga tcgagatcca gggagcgcgc caaatccggg
     2761 aggaaaggcc cgacgctgtg atgttcttca tctctcctcc atcgatcgaa gagttggaga
     2821 agaggctgat caggcggggt gacacttcag ccgccgacat cgaggatcga ctggaagtgg
     2881 ccgagaccga gatcgctgag gcgcctgaac ttttcgacca catcgtggtc aacgatcacc
     2941 tcgatcgtgc cgtagccgag atcgaggcgc tgattacggg cac
//
LOCUS       DBNHXU010000144         1575 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1631454, whole genome shotgun sequence.
ACCESSION   DBNHXU010000144 DBNHXU010000000
VERSION     DBNHXU010000144.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1575)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1575
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1631454"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(8..472)
                     /gene="ybeY"
                     /locus_tag="AC161X_03725"
     CDS             complement(8..472)
                     /gene="ybeY"
                     /locus_tag="AC161X_03725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HIR4988689.1"
                     /translation="MSVFLADEQGEELDLAGMRNLAELVLSTEGYPKETEVTLLFVDE
                     DEMSSYNERFLNRTGPTDVLAFPVEDLLPGIAPDPDPTGPPLMIGDVIIAPAYVKRQS
                     AELEVAFDDEMALMVTHGILHLMGYDHQADDAASAMEGRETEILALVGKKRR"
     gene            complement(504..>1575)
                     /locus_tag="AC161X_03730"
     CDS             complement(504..>1575)
                     /locus_tag="AC161X_03730"
                     /inference="COORDINATES: protein motif:HMM:NF014069.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HIR4988690.1"
                     /translation="VLFDARIAVLMALSVGVLTAVGTGDIGITVYAILATLAPIPFVS
                     SVSSRGAFRNAVVFSALTAAVVAASTSWFFHVGPNDVPFEVIGLSVAWAFGVSAMASL
                     IGLAALQFFESAFDITTTLGLLDLTDRNHKGLQLLQEKAFGTFNHSLMVGTLADAAAR
                     AIGANALLARAMAYYHDLGKTENPTYYIENQFGIPNPHDFLTPEESAQMIRRHVTDGV
                     ALAREFKIPSDVATGIVSHHGDGIMRFFYEKARSQRGDAVNPDDFRHVGHKPKTAESA
                     IVMLADSLEAACRAVFQTEEPTPEAIEKVVNRVIDEKLDDGQLQESPLTQFEMTQVRK
                     AFFESMVGHYHQRIAYPNFPGT"
BASE COUNT          311 a          462 c          505 g          297 t
ORIGIN      
        1 aggacggtca tcgtcgcttc tttccgacca gagcaaggat ttcggtctcc cgaccctcca
       61 ttgcggaagc ggcatcatca gcctgatggt cataccccat gaggtggagg atgccgtgag
      121 tcaccatcag cgccatttcg tcgtcaaagg cgacttccaa ctctgcggac tgccgcttca
      181 cgtaggcggg ggcgatgatc acgtcgccga tcatgagtgg aggcccggtg gggtcgggat
      241 caggagcgat accgggcaac aggtcctcca caggaaaggc aaggacgtca gtcggtcccg
      301 tgcgattcag gaagcgctcg ttgtatgagg acatctcgtc ttcatccacg aagagcaggg
      361 tgacctcggt ctcttttggg taaccctcag tggagaggac caattcggcg agattacgca
      421 tcccggcaag atccagctct tcaccttgtt cgtcggccag gaagacgctc actggccggt
      481 gttggtactg gagggctcgt ccatcaggtc ccggggaaat tcggatacgc gatccgctga
      541 tgataatgcc cgaccattga ctcgaaaaag gcctttctca cctgggtcat ctcgaactgg
      601 gtcagcggcg attcctggag ttgaccatcg tcgagtttct catcgatgac ccggttgacc
      661 accttctcga tggcctcggg agtcggctct tcagtctgga acactgcccg gcaggccgcc
      721 tccagtgagt cagcaagcat tacgatggcc gactcggcgg tcttcggctt gtgaccgaca
      781 tggcggaagt cgtccgggtt cacggcatca ccccgttggg accgcgcctt ctcatagaag
      841 aagcgcatga tgccgtcgcc gtgatggctg acaatgccgg tcgccacatc ggacgggatc
      901 ttgaactccc gggccagcgc cactccgtcg gtgacatggc gtcggatcat ctgcgccgat
      961 tcctccggag tcaggaagtc gtgcgggttg gggatcccga actggttctc gatgtaatag
     1021 gtggggttct cggtcttccc caggtcgtgg tagtacgcca tggcacgggc gaggagtgcg
     1081 ttggctccaa tggctcgcgc cgccgcatcg gccaaggttc cgaccatcag tgagtggttg
     1141 aaagtcccga acgccttctc ctggagcaac tggagaccct tgtggttgcg atcggtcaga
     1201 tcgagcagac ccagggtcgt ggttatgtcg aaggccgact cgaagaactg gagggcggcg
     1261 aggccgatca aggaagccat tgccgagacc ccgaatgccc aggccaccga gagcccgatc
     1321 acttcgaagg ggacgtcgtt gggcccgacg tggaagaacc acgatgtgga cgcggccacc
     1381 accgcggcgg tcagagccga gaacaccacc gcgttgcgga aggcgccccg gctgctcacc
     1441 gaactgacaa acggaatcgg agccagcgtg gcgaggatgg cgtagacggt gatgcctatg
     1501 tcgccggtcc cgaccgcagt gagcacacca acggagaggg ccatgaggac cgcgatccgg
     1561 gcgtcgaaga ggacc
//
LOCUS       DBNHXU010000145         3448 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_639647, whole genome shotgun sequence.
ACCESSION   DBNHXU010000145 DBNHXU010000000
VERSION     DBNHXU010000145.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3448)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3448)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3448
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_639647"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..107)
                     /locus_tag="AC161X_03735"
     CDS             complement(<1..107)
                     /locus_tag="AC161X_03735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015920310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyceraldehyde 3-phosphate dehydrogenase
                     NAD-binding domain-containing protein"
                     /protein_id="HIR4988691.1"
                     /translation="MVNVAINGFGRIGRAVFRIIASRPDSGISVVAINDL"
     gene            complement(110..1132)
                     /locus_tag="AC161X_03740"
     CDS             complement(110..1132)
                     /locus_tag="AC161X_03740"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014818.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA];
                     GO_process: GO:0006006 - glucose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HIR4988692.1"
                     /translation="MTVTRIGLMGFGRIGRNVVRLVHARDDLEVVAISDIADPEALTY
                     LLKYDSIYGRFPAPTSYADGKLNIGSKQAAFLKAREPGDANWGDLGVDVVLETTSRYR
                     SKEALDRHLAAGAKKVVLTSSPETPGEIPLLLRGINDEILGPNTNQVALGSNTSNAVA
                     PILQIIDEKFGVKRAFMTTVKAMSNAGRLADVPTDSYRTSRAAGENIIPAVTNTAEIL
                     TQALPKLEGKLAVVALNVPVVDGSTVDLTTETNVDATVEEVNAVMAAEIEARFQGVIE
                     YVADPIVSSDVRLNSHSGVFDSLATMVTMGSLLKTITWFNNGWGYSHRAVEVTTTIAG
                     YLDGGH"
     gene            1256..1528
                     /locus_tag="AC161X_03745"
                     /pseudo
     CDS             1256..1528
                     /locus_tag="AC161X_03745"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
     gene            1671..2291
                     /gene="nadD"
                     /locus_tag="AC161X_03750"
     CDS             1671..2291
                     /gene="nadD"
                     /locus_tag="AC161X_03750"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HIR4988693.1"
                     /translation="MHTGILGGTFDPIHLAHLHAGETALYQAGLDRVLFMPAGEPWQK
                     SDRALTGAAHRLEMIRLAIADIDGFEVDDREIEREGPTYTIDTLLSFPESERLSLILG
                     ADAALGLPTWQRWEEVLDRASLLVVPRPGTDAREVQAILPEAVFLDMAVLEISGTEIR
                     EMAAIGEPFRFLVPLAVYDYIESVGLYAEPGGDDMVGDSSDQEEGS"
     gene            2315..2656
                     /gene="rsfS"
                     /locus_tag="AC161X_03755"
     CDS             2315..2656
                     /gene="rsfS"
                     /locus_tag="AC161X_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016700324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HIR4988694.1"
                     /translation="MAADAIFSKKGMDIVLLDVQGLFVLTDVFVIATGTSRPHVQSLA
                     DQVEEKLREEKGIKPLRSEGRTEAEWVLLDFGDIIVHLFQAEPRDFYGLERLWADAER
                     VKWVEPAVADG"
     gene            2709..2782
                     /locus_tag="AC161X_03760"
     tRNA            2709..2782
                     /locus_tag="AC161X_03760"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2743..2745,aa:Ala,seq:ggc)
     gene            2832..3407
                     /locus_tag="AC161X_03765"
     CDS             2832..3407
                     /locus_tag="AC161X_03765"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988695.1"
                     /translation="MADYQVKPLDKETWPDFASLVERHNGVWGGCWCLEFHPEGSKKG
                     VDRRALKEQRVNENRAHAALVFDGDLCIGWCQFGSPDELPRIKFGRAYGEAAADPPDW
                     RITCFFVDKAYRHKGVAAAALKGALSEIARLGGGTVESSPEDVEGRKVSNSFLYNANL
                     AMFEKQGFERTRQLGKNSWLVTMKVVPTNSS"
BASE COUNT          608 a          997 c         1138 g          705 t
ORIGIN      
        1 aggtcgttga tggcgacgac gctgatcccg gagtcaggcc gggaggcaat gatccggaac
       61 acggctcggc cgatcctccc gaatccgttg attgcgacgt tcaccatctt cagtgtcctc
      121 catctagata accggcgatc gtcgtcgtta cttccaccgc acgatgggaa tatccccagc
      181 cattgttgaa ccacgtgatg gtcttgagca ggctgcccat cgtcaccatg gtcgccaggc
      241 tgtcgaagac gccggagtgg gagttgaggc gaacgtcgga ggagacgatc gggtcggcga
      301 catactcgat gacaccctgg aatctcgctt cgatctcggc agccatgacg gcgttgactt
      361 cttcgacggt cgcatcgacg ttcgtctcgg tggtgaggtc cacggtggac ccgtccacta
      421 ccggcacatt cagggcaacc acggcgagct ttccctcgag tttggggagg gcctgagtca
      481 ggatttcggc ggtgttggtc accgccggga tgatgttctc gccagccgct ctgctggttc
      541 gataggagtc ggtggggaca tcggcgagac gccctgcgtt ggacatcgcc ttcactgtcg
      601 tcatgaacgc ccgcttcact ccgaacttct catcgatgat ctggaggatc ggcgctacgg
      661 cgttggaggt gtttgagcca agcgccactt gattggtgtt cggcccgagg atctcgtcgt
      721 tgatcccacg aagcaagaga gggatctcgc cgggggtctc cgggctcgag gtgagaacga
      781 ctttcttggc tccggcggcg agatggcggt caagggcttc cttggagcga tagcggctgg
      841 tcgtctccag caccacgtcg acgccgaggt caccccagtt ggcgtcgccc ggctccctcg
      901 ctttcaggaa ggcggcctgc ttcgacccga tgttgagctt tccgtctgcg taggaggtgg
      961 gcgcggggaa acgcccgtag atcgagtcgt acttgaggag gtaggtgagc gcctcgggat
     1021 ccgcgatatc gctgatggca accacttcca ggtcgtcgcg ggcgtgaacc agtcggacaa
     1081 cattgcgccc gattcttccg aaacccatca acccgatgcg ggtcaccgtc attcagtctc
     1141 ctctctggag tatgaaaact agcggtcggc cctctgtcta tctcaatcca tcggcgatag
     1201 gttcggagca ggaggggaga tgctgatcca attggcatta gccgcggttt gcctgatggc
     1261 gcctgttgag ggacccgtcg tcgccggtta cgcgccgatc ggctcctatg gcgggcattg
     1321 gggtgtggat tacttcgccg cggtggggtc gtcggttcgt gctccggcgt ccggggaggt
     1381 gacgtttgcg gggacggtgg ccggcatggt gtcggtgacc atcgaaccga tccccggatt
     1441 caaggtctcc atttcctacc tctccgatcg cacagtgcgc tcgggtcagc aagtgaccag
     1501 aggagccatc atcgggaccg cgggcactcc ccatggtcgg cccggagtgc atctctcgac
     1561 ccgggtaggt ggcagctacg tggacccgcg ctcaaggctc ggttgccact cgaccgacat
     1621 cagtcgtgcg cttcggctgg tgacacctcc ctcgccctat cctcgacgtc gtgcacaccg
     1681 ggattctcgg ggggaccttc gacccgatcc atctggctca tctccacgcc ggggagacgg
     1741 cgctgtacca ggccggcctc gatcgagtct tgttcatgcc ggcgggggag ccctggcaga
     1801 agagtgatcg ggccttgacc ggtgccgccc atcggttgga aatgatccga ctcgcgatcg
     1861 ccgacatcga tggcttcgaa gtagacgatc gagagatcga gcgagagggg cccacttaca
     1921 cgattgacac cctcctcagc tttcccgaga gcgagcggtt gtccttgatc ctgggggctg
     1981 acgccgccct gggtctgccg acctggcagc gatgggagga ggtcctagac cgagcgtcgt
     2041 tgctcgttgt tcccagacca ggcaccgatg cccgggaggt ccaggcgatc ctgccagagg
     2101 ctgttttcct cgacatggcg gtgctcgaga tcagtggcac cgaaatcaga gagatggctg
     2161 cgatcggcga gccgtttcgg ttcttggtgc cgttggctgt gtatgactac atcgagtcgg
     2221 tcgggctgta tgcggaaccc gggggagacg atatggtggg ggactcatcc gatcaggagg
     2281 aaggctcgta gagaacagtg cggaacttgc ccgggtggcg gccgacgcca tcttcagcaa
     2341 gaagggcatg gacatcgtgc tgctcgatgt tcaagggctg ttcgttctca ctgacgtctt
     2401 cgtgatcgcg accgggacct cgcgtcccca tgttcagtct ttggccgatc aggtcgagga
     2461 gaagctccgt gaggagaagg ggatcaagcc gctgcgcagc gagggtcgca ccgaggcgga
     2521 gtgggtgctc ctcgactttg gtgacatcat cgtccacctg tttcaggcgg agccccggga
     2581 cttctacggc ttggaacggc tgtgggccga cgccgagcgg gtcaagtggg tggagcccgc
     2641 ggtagccgac ggataggccg ggttacccct tggggccggg gctcgaataa actcccgttt
     2701 cccacgatgg ggctgtagct caggttggca gagcacttga ctggcagtca aggggtcagg
     2761 ggttcaaatc ccctcagctc cactccgttc gatcgcaatt gcttagtcgt ctcacaggta
     2821 gctttccctt gatggccgac tatcaggtca agccgctgga caaggagaca tggcccgatt
     2881 tcgccagtct cgtcgagcgt cacaacggtg tttggggtgg atgctggtgt ctggaattcc
     2941 atcccgaagg ctctaaaaag ggcgttgatc gacgtgccct gaaggagcag cgggtcaatg
     3001 agaaccgagc tcatgcggcc ctcgtgttcg atggggatct gtgcatcggg tggtgccagt
     3061 tcggctcgcc ggacgagttg ccacgcatca agttcgggcg ggcctacggc gaagccgccg
     3121 cagatccccc agattggcgg atcacctgct tcttcgtgga caaggcatac cggcacaagg
     3181 gggtcgccgc cgccgcgctc aaaggtgctc tgagcgagat cgcacgactc ggtgggggga
     3241 ccgtggagag ctctccggag gacgtcgagg ggcgcaaggt ctccaactca ttcctgtaca
     3301 acgccaacct ggcgatgttt gagaagcaag ggttcgaacg cacccggcaa ctcggaaaga
     3361 acagttggct ggtcaccatg aaggtggtgc cgacgaactc gagctgacca agtccaccag
     3421 cttctatggt ccctggatgg cgagatct
//
LOCUS       DBNHXU010000146         4729 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4212012, whole genome shotgun sequence.
ACCESSION   DBNHXU010000146 DBNHXU010000000
VERSION     DBNHXU010000146.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4729)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4729)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4729
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4212012"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="AC161X_03770"
     CDS             complement(<1..865)
                     /locus_tag="AC161X_03770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009571730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR4988696.1"
                     /translation="MTKRVIALTAVLMIILAACGGDDATSSTTAAGDGTTVAGGGDCT
                     VGMSWNNYNEERWAKHDEPAIKAALEAGGCDYISADAGSSEEQQITDVEKLISDGADV
                     VIILAQNTQAILPAVEAATAEGIPVIAYDRLIEDANTFYVSFDNVLVGQLMAEVIFDL
                     VPTGNYIIVKGNGSDANSDFLREGIESVIGDAVAAGDITICCEDYTDNWDPALAQTAV
                     EQWLTQENNEVDAVVSENDGMATGAAAALAAQGLSIPLSGQDADVAALNRVALGTQSV
                     SVWKDARELGKA"
     gene            956..2656
                     /locus_tag="AC161X_03775"
     CDS             956..2656
                     /locus_tag="AC161X_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR4988697.1"
                     /translation="MTNLKSESKVDSGRRISSNVWLAAGVVTGSLVVIGALSLGRVIV
                     DASGPPDALPAPHYLEEATTAGVTHVYDGEFTFFVGGGVAVFDCNQDSRPDLYFAGGV
                     NPASLYVNASPIAGELAFEQVAGPTTDLTSVAGAYPLDVDGDGLVDLAVLRVGENVML
                     RGLGNCRFERANEAWGIDGGNEWTAAFSAQWEGAAVLPTLAFGNYVALTESGERAGGC
                     SDSMMLRPAGNSTYGSPITLSPGWCTLSVLFSDWDRSGRRDLRMTNDRHYYRDGEEQL
                     WQVAPGEPPRPYTREDGWQKMSIWGMGIASYDLTGDGLPEVFLTSQGDNKLQTLVDGP
                     AQPNYADIALDRDANAHRPFTGDDTLMPSTAWHAEFQDVNNDTFIDLFVAKGNVDAMA
                     EFAADDPSNLMLGGPEGTFTEGADDAGIVNFAQGRGAALADLNMDGLLDLVEVNRREN
                     VKMWRNVGSGDAGKSEPLGNWVALQLEQPGPNRDAIGSWIEVKAGSQTAERELTIGGG
                     HAGGQLGWVHFGLGAAEEAEIQVQWPDGEKGPWMTVDANQFLTIDRDDAEARPWQGQD
                     "
     gene            2658..4034
                     /locus_tag="AC161X_03780"
     CDS             2658..4034
                     /locus_tag="AC161X_03780"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006333107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR4988698.1"
                     /translation="MSGARISEIALPEFGMPDAMPEIPSEIYVDRLARLRERAVERGY
                     DHLIVYADREHSANLSYLTGFDPRFEEAVLIVGPTGDPAILVGNECYGMAGAAPLAMR
                     RHLFQDLSLPGQPRHRSQPLPEILADEGMRRGSHVGMIGWKSYSDRELMEVPAFIVDV
                     VRRLTGPTGTVENANDLLIGAGDGLRVINEVEQLAALEYAACQTSNGVRQLLVGLEPG
                     MTEQEAVRLLGWNGMPLSCHLMLTAGPRATLGLLSPGDRPIGRGDRFTVAFGIWGALN
                     CRAGFVVEDAAELPEEISDYVERLVGPYFEAVAEWYGAMRIGQPGRVFQEIIDRHLGD
                     PFFGIFLNPGHQIHLDEWVNSPIAPGSQTELRSGMAFQVDIIPATGTSYFTTNIEDGI
                     ALADESLRAEFSGRYPGAWARIQARRDFMGRGLGIDLHPDVLPFSNIPAFLPPFLLSP
                     DRVMTVAG"
     gene            complement(4031..>4729)
                     /locus_tag="AC161X_03785"
     CDS             complement(4031..>4729)
                     /locus_tag="AC161X_03785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR4988699.1"
                     /translation="QLGEIRLVQVEYVQGGKAGENDPDPKHGLPWRYDPVRGGPSLVM
                     GDIGSHAHNLARFVTELEVLEVSAEIGAIVPNRLVHDYGAAMLRFDNGARGSLWVTQA
                     AAGVENCLRIRVSGTRGTLEWEQEDPTVLVFKPLVGPAEVRTLNGPGTLPLSARATRI
                     VPGHPEGFHEAFANLYADAAEAIAARRTGVQPDPLALQFPNEVDGAIGVKFVDAAIAS
                     STQSGAWTNALLDL"
BASE COUNT          884 a         1501 c         1480 g          864 t
ORIGIN      
        1 cagccttgcc cagctcacga gcgtccttcc acaccgacac cgactgcgtt cccaacgcca
       61 cccggttgag ggccgcgacg tctgcgtcct gacccgacaa cgggatgctg aggccctggg
      121 ccgcgagagc ggcagcggcg ccagtcgcca ttccgtcgtt ctcggacacc acggcgtcga
      181 cctcgttgtt ctcctgagtc agccactgct caactgcggt ctgagcgagt gcgggatccc
      241 agttgtccgt gtagtcctca caacaaatgg tgatgtcgcc tgccgcaact gcgtcaccaa
      301 tgaccgactc gattccctca cggaggaagt cagagttggc gtcagagccg ttgcccttga
      361 cgatgatgta gttgccggtc ggcaccaggt cgaagatgac ttccgccatc aactggccca
      421 ccaggacgtt gtcgaagctg acgtagaagg tgttcgcatc ctcgatcaga cggtcatagg
      481 caatcacggg gattccttcg gccgtagcgg cttcgaccgc agggaggatc gcttgcgtgt
      541 tctgagccag gatgatgacc acgtcggcgc catcagagat cagcttctca acgtcggtga
      601 tctgctgctc ctcggatgac ccggcgtctg cggagatgta gtcgcaaccg ccggcctcta
      661 gagcggcctt gattgcgggc tcatcgtgct tggcccatct ctcttcgttg tagttgttcc
      721 acgacatgcc gacggtgcaa tcgccgccgc cagccaccgt ggtgccatcg ccagccgcag
      781 tcgtcgagct ggtggcatca tcgccgccac aggcggcgag aatgatcatc agcactgcag
      841 tcagcgcaat gactcgtttc gtcatcagaa ttagtttcct tcctcctctg gatgatgtcc
      901 ccgaacggga ccggcttact gtagccatac ctgctcgaag cacttcacca aatccatgac
      961 gaacttgaaa tcagaatcca aggtcgattc gggccgacga atctcctcga acgtgtggtt
     1021 ggcggcggga gtggtcaccg ggtcgctggt cgtcatcgga gccctgtcgc tgggccgggt
     1081 gatcgtcgat gccagcggtc ccccagatgc cctgcccgcc ccgcattacc tcgaggaggc
     1141 aaccacggcc ggggtcaccc atgtctacga cggggagttc acattcttcg tgggtggcgg
     1201 agtcgccgtg ttcgactgca accaggacag ccgacctgac ctctacttcg caggcggggt
     1261 caacccagcc tcgctgtatg tcaatgcgag cccgatcgca ggcgagctgg ccttcgagca
     1321 ggtggctggc ccaaccaccg acctcacatc ggtcgccggg gcctacccgc tcgatgtgga
     1381 cggggatggc ctcgtggatc tcgctgtgct gcgtgtcggc gagaacgtga tgctccgtgg
     1441 tttgggcaac tgccgcttcg agcgcgcaaa cgaggcttgg ggcatcgacg ggggaaacga
     1501 atggaccgcc gctttcagcg cccaatggga aggtgcggcc gtgttgccga ctctggcgtt
     1561 cggcaactat gtcgccttga ccgagagtgg cgagcgagca ggaggctgct ccgacagcat
     1621 gatgttgagg ccagccggga actcgactta tggatcaccg atcacgctgt caccgggatg
     1681 gtgcaccctc tccgtcttgt tcagtgactg ggatcgctcc ggtcgccggg acctgagaat
     1741 gaccaacgac cgccactact accgagatgg cgaggagcag ttgtggcagg tggctccggg
     1801 cgagccacca cgcccctata cccgagaaga tggatggcaa aagatgagca tttggggcat
     1861 gggaatagcc agctatgacc taaccggtga cgggctccct gaggtatttc tcaccagcca
     1921 gggcgacaac aaactgcaaa ctctggtcga cggccccgcc caaccgaact atgcggacat
     1981 tgccctcgat cgtgatgcca atgcccaccg accgttcacc ggtgacgaca ccctgatgcc
     2041 gtccaccgct tggcacgccg agtttcagga cgtgaacaac gacactttca tcgacctgtt
     2101 cgtagccaaa ggaaacgttg acgccatggc cgagttcgcc gccgacgatc cgagcaacct
     2161 gatgcttgga ggccccgagg ggactttcac cgagggagcc gatgacgccg ggatagtcaa
     2221 cttcgcccag ggccgagggg cggccctcgc cgatctcaat atggatggtc tcctcgacct
     2281 cgtggaagtc aaccgccggg agaacgtcaa aatgtggcgc aacgttggat ccggagacgc
     2341 agggaaatcg gagccgcttg gcaattgggt ggcactgcaa ctcgagcaac cgggaccgaa
     2401 tcgcgacgcg atcggttctt ggatcgaggt caaggctgga agccagaccg ccgaacgcga
     2461 attgacgatc ggaggcgggc atgccggcgg gcagttgggt tgggtccatt tcgggctcgg
     2521 ggcggcggag gaggcggaga tccaagtcca atggcccgac ggggaaaagg ggccgtggat
     2581 gaccgtcgac gccaatcagt ttctcactat cgatcgggac gacgctgaag ctcgaccctg
     2641 gcaagggcag gattgacatg agcggagcac gcatctctga gattgcgctc ccggagttcg
     2701 ggatgcccga cgcaatgccg gagatcccat cagagatcta cgtcgaccgg cttgcccgcc
     2761 tcagggaacg ggccgtggag cgcggctacg accacttgat cgtgtacgcc gatcgcgagc
     2821 acagcgccaa cctctcctac ctgaccgggt ttgacccccg attcgaggaa gccgtgttga
     2881 tcgttggacc gacaggcgac ccagcgatcc tcgtcggcaa cgagtgctac ggcatggccg
     2941 gggcggcgcc cctagccatg cgccgacatc tgtttcagga cctcagcctc cccggtcaac
     3001 cgagacaccg ctcacagccg ttgcccgaaa tcttggctga cgagggcatg cgtcgtggaa
     3061 gccatgtggg aatgatcggt tggaagagct attccgaccg tgaactgatg gaggtcccgg
     3121 ccttcatcgt cgatgttgtg cgtcggctga ccggaccgac agggacagtc gaaaacgcga
     3181 acgaccttct gatcggagcc ggtgacgggc tgagggtcat caacgaggtc gagcaactcg
     3241 ccgccctcga atacgccgca tgtcagacct ccaacggagt gcgacaactg ctggtcgggt
     3301 tggaaccggg gatgacagag caggaggcgg tccgactact cggatggaac gggatgcccc
     3361 tttcctgtca cctcatgctc accgccggcc ccagagcgac ccttggtttg ctgagccccg
     3421 gtgatcgccc gatcggccgt ggtgatcggt tcacagtcgc cttcggaata tggggagcct
     3481 tgaactgtcg ggccgggttc gtcgtcgagg acgctgcaga actacccgaa gagatcagcg
     3541 actacgtcga acggctggtc ggcccatatt tcgaggcggt ggctgagtgg tacggggcga
     3601 tgcggatcgg ccaaccggga cgggtgttcc aggagatcat cgaccgtcat ctaggtgacc
     3661 ccttctttgg gatcttcctc aatcccgggc atcagatcca cctcgacgag tgggtcaact
     3721 ccccaatcgc ccccggctcc cagaccgaat tgcgctcggg gatggccttc caggtggaca
     3781 tcatcccggc caccggcacg agctatttca cgaccaacat cgaagacggc atcgccctcg
     3841 ccgacgagag cctgcgggct gagttctccg ggcgttaccc gggcgcgtgg gccaggatcc
     3901 aggctcgtcg cgatttcatg ggcagaggcc tcggcatcga cctccatccc gacgtgttgc
     3961 cgttctccaa catcccggcc tttctccccc ccttcttgct cagccctgat cgggtgatga
     4021 cagtcgccgg ctagagatcg agaagggcgt tggtccacgc cccactttga gtgctggatg
     4081 cgatggcggc gtccacgaac ttgaccccga tcgccccatc cacctcgttg gggaattgga
     4141 gtgccagcgg gtcgggctgg accccggtgc gccgggcggc gatggcctcc gccgcatcgg
     4201 catagaggtt ggcaaacgcc tcgtgaaacc cctccgggtg tccgggcacg atccgggtgg
     4261 cccgcgccga aaggggcagg gtgcccggcc cgttcaaggt cctgacctcg gccggcccaa
     4321 ccaagggctt gaaaacgaga accgtcggat cctcttgctc ccactcgaga gtgcccctgg
     4381 tacccgacac ccggatccga aggcaattct cgactcccgc cgctgcctgg gtaacccaca
     4441 agctgccccg ggcgccgttg tcgaagcgca acatggcggc gccgtagtcg tgaaccaggc
     4501 ggttgggaac gatggccccg atctcggcag agacctccaa gacctcgagc tccgtcacga
     4561 accgggccag gttgtgtgca tggctaccga tgtcacccat caccaacgat gggccgcccc
     4621 gaaccgggtc gtagcgccag ggcagcccgt gcttggggtc ggggtcgttc tcgcctgctt
     4681 ttccgccctg gacatactcc acctgcacca gtcggatctc cccgagctg
//
LOCUS       DBNHXU010000147         3360 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5404375, whole genome shotgun sequence.
ACCESSION   DBNHXU010000147 DBNHXU010000000
VERSION     DBNHXU010000147.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5404375"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="AC161X_03790"
     CDS             <1..188
                     /locus_tag="AC161X_03790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988700.1"
                     /translation="NVPLLDQIGITEARFNTVIGLLVLLIMILSPEGIMGIVDRLGRG
                     GRGAEPSVPSIAKVETG"
     gene            284..1774
                     /locus_tag="AC161X_03795"
     CDS             284..1774
                     /locus_tag="AC161X_03795"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4988701.1"
                     /translation="MRPAWWLVILTVLALTLAACGEDATDGSTTSTGATATTEAPTGT
                     TSAPGTTVAGGEAIKLGILAECEGAFGGFNEDVVAGVVLGLINGAGATSVSRTTALEG
                     WTGAVAGGREIELVGIGCGDDTADKIIQEVRKLVEENGAEVVIGPLSGDESIAIANYA
                     KDHPEVTFLSGIGGAQETTLQVQAPNFFRFHGDGAQWNAGLGDLLHNTAGWDTAAVIA
                     DDYSFGWTSSGGFIADFCAVGGTIAPEHRIYPPLGTTDYSSFIQQLPDPDEVDGYFWV
                     VGGTGTQAALEAFVNAKGELTGAQHAGNLFFNPGLAAALGPNIAGSYVGGFATPPGDV
                     VTPEITAYLDSANAAWDTLAGALTGNEPGDPSIAAGFGFFYGFYSGTTALVNALNAVD
                     GDLSNNHEALRAELSGMTADLPYGPVTLDENRQGIVDVYLSQLVLDGEDVVQSTVALI
                     PQVDQTFGGTFSGDTPGPSRDFPGCETADLPWAGNAIPVVDGVPQG"
     gene            1851..2579
                     /locus_tag="AC161X_03800"
     CDS             1851..2579
                     /locus_tag="AC161X_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988702.1"
                     /translation="MTKAFGGLVAVDGVDLDVAHGQRLGLLGPNGAGKTTLFNLIAGD
                     LRPTSGTIRIGGVDCTMLASRHRASFGVARTYQRTRLFGGLSVADNLYLAQTGKSGRH
                     RALWRSAVDDVFEEKAHAAADKVWLGKRLADTVDDLSHGERRQLEVAMALVTDPAILL
                     LDEPASGLSQGERENLTQLLLGLPRELTLILIEHDMDVALKAAEQIVVMSDGRVVASG
                     TTEEIRHDPVVHEIYLGEAERRHR"
     gene            2576..3286
                     /locus_tag="AC161X_03805"
     CDS             2576..3286
                     /locus_tag="AC161X_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988703.1"
                     /translation="MTDQLLEVEGLEAGYGQSRVLDGISFSMGTESVAIVGRNGMGKT
                     TLCNALMGLIRPTAGSVIFDGVVLNGLTPEKISRVGIGYVPQGRRLFPSLTVDEHLRM
                     LAKDARGKRWTADSVYELFPRLAQRRQNRGGELSGGEQQMLAVGRALMLNSKLLLMDE
                     PSEGLAPTIVDTLIASVIALVDEGVAVLAVEQNIHAATAMADRQLAMVSGRIEAEFTA
                     EELRDDPKLQRQYLGVDA"
BASE COUNT          641 a         1015 c         1097 g          607 t
ORIGIN      
        1 gaaacgtccc gctgctcgat cagatcggta tcacggaggc tcgattcaac acagtgatcg
       61 gtctcctggt gctcctgatc atgatcctct cacctgaggg gatcatgggc atcgtcgacc
      121 gacttggccg tggtggccgg ggcgccgagc cttcggtgcc ttccattgcg aaagtcgaga
      181 caggttgatg ctgaagtcgg cggacgaaac aaaaggaaag gtgaggggca ccagcaactc
      241 accgttatga ggaggaaaga tgagagcatc taacacggga cgtgtgaggc cagcctggtg
      301 gctggtgatc ctcaccgtcc tcgcgttgac gctggccgcg tgtggcgagg atgccaccga
      361 cgggagcacc acgtcgacgg gggccaccgc aaccacggag gccccgacgg ggaccacgag
      421 cgctcccggc accacggtcg ccggaggtga agcgatcaag ctcggcatcc tcgccgagtg
      481 tgagggggcc ttcggtggct tcaatgaaga cgtcgtagcc ggagtggtgc tgggcctcat
      541 caatggtgcc ggggccacca gcgtttcgcg gacgaccgct ctcgaagggt ggaccggcgc
      601 ggtcgccggt ggtcgcgaga tcgaactggt tggcatcggc tgcggtgacg acaccgccga
      661 caagatcatc caggaagttc ggaaattggt cgaggagaac ggtgccgaag tcgtgatcgg
      721 cccgctctcg ggcgacgagt cgatcgccat cgccaactat gcgaaggatc atcctgaggt
      781 gaccttcctc agtggcatcg gcggcgccca ggagaccaca ctgcaagtgc aggctcccaa
      841 cttcttcagg ttccacggcg acggcgccca gtggaatgca ggtctgggtg acctcttgca
      901 caacacggcc ggatgggaca ccgcagcggt catcgcggac gactacagct ttggctggac
      961 gtcatctgga ggattcatcg ctgacttctg tgcggtcggt ggaaccatcg cacccgagca
     1021 tcggatctat ccgccattgg ggacgacgga ctactcgtcg ttcatccagc agctccccga
     1081 ccccgatgag gtcgacggct acttctgggt tgtcggcgga accggtaccc aggcagcact
     1141 cgaggcattc gtcaatgcca agggcgaact gacaggggcc cagcatgctg gaaacctgtt
     1201 cttcaacccc ggtctggcgg cagccttggg tccgaacatt gcgggttcct acgtgggtgg
     1261 attcgccact ccccccggcg atgtggtcac gcctgagatc acggcgtatc tggacagtgc
     1321 caacgcggcc tgggataccc tggctggagc cttgaccggc aacgagccgg gcgacccatc
     1381 gatagccgcg ggattcgggt tcttctacgg gttctacagc ggcaccacag cgctggtcaa
     1441 cgcgcttaat gccgtcgatg gtgacctgtc gaacaaccat gaagcgcttc gcgccgaatt
     1501 gtccgggatg actgccgacc tgccgtatgg cccggtgacg ctcgacgaga accggcaggg
     1561 catcgtcgac gtctacctct cgcagttggt cctcgatggc gaagacgtgg tccaaagcac
     1621 cgtcgcgctc atccctcagg tcgaccagac attcggagga acgttcagtg gcgatacacc
     1681 aggaccgtcg cgagacttcc ccgggtgtga gactgcggat ctgccgtggg ccgggaatgc
     1741 aattcccgtg gtcgacggcg ttccccaagg ataagtaagg cgccttcgtg gggcccagtc
     1801 ccgattcacc gaccgaaacc gctcagggcg aggccctcgg gcttcggtcg gtgacgaagg
     1861 cattcggagg ccttgtggcc gtggacggcg tcgatctcga tgtcgcccac ggccaacgcc
     1921 tcggcctcct cggccccaac ggcgctggga aaacgaccct cttcaatttg atcgctggcg
     1981 acctgaggcc gacctcggga acgatccgaa tcggaggcgt ggattgcacg atgttggcat
     2041 cacgccatcg ggcaagtttc ggagtcgctc gcacctacca gaggacgcgt ctgttcggcg
     2101 gtctgagcgt ggcggacaat ctctatttgg cgcagaccgg caagagcggc cgtcatcgag
     2161 cgttgtggcg atcggctgtt gacgatgtct tcgaggagaa agctcatgcc gcggctgaca
     2221 aggtgtggct gggcaaacga ttggcggaca cggtggacga cctgtcccac ggggagcggc
     2281 gtcagctcga ggtggccatg gccctggtca cagaccccgc catcctcctg ctcgacgagc
     2341 cggcctccgg cctctcgcag ggagagcgcg agaacctgac ccagctgctg ttgggcttgc
     2401 cccgtgagct gaccctcatc ctcatcgagc atgacatgga cgtcgcgctc aaggcagccg
     2461 agcaaatcgt ggtcatgtcc gatggcaggg tggttgcgtc gggcaccact gaggagatcc
     2521 gtcacgatcc agtggtccac gagatctatc tgggcgaagc ggagaggcgt caccgatgac
     2581 cgaccagcta ctcgaagttg aggggttgga ggccggatac ggccagtcgc gggtcctcga
     2641 cgggatctcc ttctcgatgg gcacagaatc ggtggcgatc gtcggccgca acgggatggg
     2701 caagacgacg ttgtgcaacg ctctcatggg gctaatccga cccactgcgg gatcagtgat
     2761 ttttgatgga gtggtgctca atggtttgac gccggaaaag atttcgcgcg tcggaatcgg
     2821 ctacgtccct cagggccgac gtctgtttcc ctcgctcacc gtcgacgagc acctccgcat
     2881 gttggccaaa gacgccaggg gaaagcggtg gacagccgat tccgtctatg agttgttccc
     2941 gcgactggcc cagcgccgcc agaatcgcgg cggagagctc tccggcgggg agcagcagat
     3001 gctcgccgtg ggcagggcgc tgatgttgaa cagcaagctg ctcctcatgg acgagccatc
     3061 cgaagggctc gccccgacaa tcgtcgacac cctcatcgcc tcggtgatcg ccttggtaga
     3121 tgaaggcgtc gccgttcttg ccgtcgaaca gaacattcac gcagccacag ccatggctga
     3181 tcgtcaacta gcgatggtgt ccggccgcat cgaggccgag ttcacggccg aggagcttcg
     3241 agatgacccc aagctgcaac gccagtatct gggtgtcgat gcctagccac ctaaccgagg
     3301 agaggaatag cgctcaatga ccatgaccaa catcgccttg ctgggctcca agttcatggg
//
LOCUS       DBNHXU010000148         3887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2624661, whole genome shotgun sequence.
ACCESSION   DBNHXU010000148 DBNHXU010000000
VERSION     DBNHXU010000148.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3887
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2624661"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="AC161X_03810"
     CDS             complement(<1..191)
                     /locus_tag="AC161X_03810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988704.1"
                     /translation="MRFRTLAVALLAALALALAACSGGGGTTVTEGQDVTVQMFDNRY
                     EYTEIHVPPGGSVTFVGAGR"
     gene            complement(188..745)
                     /locus_tag="AC161X_03815"
     CDS             complement(188..745)
                     /locus_tag="AC161X_03815"
                     /inference="COORDINATES: protein motif:HMM:NF017999.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4988705.1"
                     /translation="MNIKQDFLVARPANVVWDFFQDVPDVAQCLPGANLTGQTEDGSY
                     EGNLSVKLGPMAAAFEGNCVVLPDHSSMTATIEGKGVDKKGGSRGQVKVVYTITPEGE
                     GSKVLVDADVTLSGPAAQFGRTGLINEMSRRLISEFVACLEAKLAAPTVEEAESIQAG
                     EVRGVSLFFASLWSWFKGLFSKGDK"
     gene            complement(805..1263)
                     /locus_tag="AC161X_03820"
     CDS             complement(805..1263)
                     /locus_tag="AC161X_03820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008339202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HIR4988706.1"
                     /translation="MITVSDGVSSGTREDKSGEALETLLSDTGFEVRRMVVPDEIPAI
                     VTAISEAAREAVLIVTTGGTGFGPRDVTPEATVQVLEREAPGLTHMMMARGLESTAMA
                     GLSRARAGTIGSTLVINVPGSSKGAVESLEAILHVIPHALDLLSGDTEHR"
     gene            complement(1281..2123)
                     /locus_tag="AC161X_03825"
     CDS             complement(1281..2123)
                     /locus_tag="AC161X_03825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988707.1"
                     /translation="MTSEVTAQESTATETIKRIGPPLVVASILGSTMGIVVAQFLDDF
                     GDGLLARILGGSAVVYNNRTEITGATDLAWAGGFFLCLIVGFILLFSYPTARGHGTAR
                     LTMLWMLLNILRQAFAQAILLPFDSGSQLAKAYATLDAPAGLDVVISAGGGVGLLLIV
                     LSAAAAFLAFTPHHRLITTGRKRFNFALWIALVPAVVSVFLAIPFFTPDAGSGVIPSL
                     PLTAVAFLFTVAAAPGTTTVHGPDEPQVTPWPWGLGGTLFVILLFEIFVLRGGVSIDP
                     TQWG"
     gene            complement(2196..2453)
                     /locus_tag="AC161X_03830"
     CDS             complement(2196..2453)
                     /locus_tag="AC161X_03830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988708.1"
                     /translation="MTEPTPRQVLYGLVAGGFFVVVLALIVGAGVTGVVPLPWILTMG
                     ALWLILAVWSAFNWRQTARVLLFSIGLFLFWAVATVLIVNS"
     gene            complement(2450..3013)
                     /locus_tag="AC161X_03835"
     CDS             complement(2450..3013)
                     /locus_tag="AC161X_03835"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008263 - pyrimidine-specific mismatch
                     base pair DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006285 - base-excision repair, AP site
                     formation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mismatch-specific DNA-glycosylase"
                     /protein_id="HIR4988709.1"
                     /translation="MGFTQAQLKAYEGQTVPDLVAPGIKLLFVGINPGLWTAATQTHF
                     CHPTNRFYPALRLAGLIDIEFDTDVGMSDSQRAQFLSRGIGITNLVARATARASEIGS
                     DELRVGGERLRATIGDLNPSVVAVAGVTAYRTAFGESEAMLGRQKGDLAGAETWVLPN
                     PSGLNAHETIATLSLWFVEVGKRARLL"
     gene            complement(3021..>3887)
                     /locus_tag="AC161X_03840"
     CDS             complement(3021..>3887)
                     /locus_tag="AC161X_03840"
                     /inference="COORDINATES: protein motif:HMM:NF014708.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HIR4988710.1"
                     /translation="IREGRSATVGATTVTRILPTKGRRTVGPWCFVDLILPPDFEDPH
                     PLEIGPHPHTNLATVTWLLEGSALHTDSLGTEQLIHPGQLNLMTAGHGIAHAELGTTT
                     GIHGVQMWLAQPEETRHGPSRFQHVAELPVVDFATAEATVMVGTLGAVESPAHVDTLM
                     VGADVKARHGLLEVPLQTAFEYAVVPIDRSLKVGEDIVEPGWLAMVPPGGDQITIDVG
                     PNGGRFLLLGGEPLGEPIEMWWNFVARSKDEITAAWRAWQTHDEEWFGPVPSKLGRID
                     APIPPWLPASEA"
BASE COUNT          768 a         1221 c         1250 g          648 t
ORIGIN      
        1 cggccggcgc cgacgaaggt caccgaaccg cctggcggaa catgaatctc ggtgtactca
       61 tatcggttgt cgaacatctg aacggtgaca tcctgacctt cggtgactgt ggttccacct
      121 ccgccgctgc atgcagcgag cgcgagcgcg agcgcggcga gcagggcaac ggccagagtc
      181 cggaatctca cttgtcacct ttcgagaaga gccctttgaa ccaactccac aatgaggcga
      241 agaagaggga gacgccacga acctccccgg cctgtatcga ctcggcctcc tccaccgtgg
      301 gcgcagccaa cttggcttcg aggcaagcca cgaactcgct gatcaggcgt ctcgacatct
      361 cgttgatgag cccggtacgg ccgaattggg cagccgggcc ggacaatgtg acatcggcgt
      421 cgaccagcac tttcgatcct tcaccctccg gagtgatggt gtaaaccact ttcacctgac
      481 cccgggagcc acctttcttg tcgactccct tgccctcgat tgtggcagtc atcgaggaat
      541 ggtccgggag cacgacgcag ttgccctcga aggcggccgc catgggtccc agcttgacgg
      601 aaaggtttcc ctcgtacgag ccatcttcag tctgtccggt gagattggcc ccgggcaggc
      661 actgggccac atcgggcacg tcctggaaga aatcccagac gacgttggcg ggacgcgcca
      721 ccaagaaatc ctgcttgatg ttcatgatcc tcctgcgagg tggtcgaaga agcggtcaga
      781 cggtagtcat ccaatgccgg tagatcaccg atgctcggtg tctcccgaca gcaggtcgag
      841 ggcatgcgga atgacgtgga ggatcgcctc gaggctctcg accgccccct tggatgaccc
      901 gggcacgttg atcacgagag tgcttccaat cgtgccggcc cgtgcccgtg agagacctgc
      961 catcgcggtg gactccagtc ctcgtgccat catcatgtgg gtcagtcccg gtgcctcccg
     1021 ctcgaggact tggacggttg cctcgggcgt cacatctctt ggcccgaatc cggtgccgcc
     1081 ggtggtgacg atcagaacag cctcacgggc cgcctcgctg atcgcagtga cgatggccgg
     1141 gatctcatcc gggaccacca ttcgtcgcac ctcgaacccg gtgtccgaaa gcagtgtttc
     1201 cagagcctcg ccggacttgt cctccctggt gccggagctg acaccgtcgc tgacggtgat
     1261 cacgaacgcg gtaggggtcg ctacccccac tgggtggggt cgatcgacac tccgccgcgg
     1321 agaacgaaga tctcaaagag gaggatgacg aaaagcgtgc ctcccaggcc ccatggccag
     1381 ggcgtgacct gtggctcgtc gggcccatgg accgtcgtcg tacccggtgc cgccgccact
     1441 gtgaaaagga aggcaacggc ggtcaacggc aaggagggga tgaccccgct cccggcgtcg
     1501 ggcgtgaaga atgggattgc aaggaacacc gagaccacgg cgggcaccag ggcgatccag
     1561 agtgcaaagt tgaagcgctt gcgcccggtc gtgatcagcc ggtgatgcgg agtaaaggcg
     1621 aggaatgccg ccgccgcgga aaggacaatc aggaggaggc cgaccccgcc tccggctgag
     1681 atgaccacgt cgaggccggc gggggcatcg agcgtggcgt atgccttggc cagttggctg
     1741 ccactgtcga aggggagaag gatggcttgg gcgaatgcct ggcgcaggat gttgagcagc
     1801 atccagagca tcgtgaggcg agcggttccg tggccacggg cggtcgggta tgagaagaga
     1861 agtatgaacc cgacgatcag gcagagaaag aatccgcctg cccatgccag gtcggtcgcc
     1921 cccgtgatct cggtccggtt gttgtagacg acggcactac caccgaggat cctcgccaga
     1981 agcccgtccc cgaagtcgtc gaggaactgg gccacgacga tccccatggt cgaaccgaga
     2041 atggatgcca cgaccagcgg aggcccgatc cgcttgatcg tctccgtggc cgttgactcc
     2101 tgagcagtta cctcactcgt cacgcaccgt gatgctaaac gacagcttcc acatgaccag
     2161 accttccctt tccgcaccca aatccgaatt ttcggtcagg aattgacgat caggactgtg
     2221 gcaacggccc aaaaaaggaa caaaccgatc gaaaacagca ggacacgggc ggtctgtctc
     2281 cagttgaacg ccgaccaaac ggcgaggatg agccacaaag cgcccatcgt cagtatccac
     2341 ggaaggggga ccacgccggt cacaccggca ccgacgatca gcgccagaac gacgacgaag
     2401 aagccgccgg caaccaggcc atagaggacc tggcgaggag tgggctcggt cacaacagcc
     2461 tcgcccgctt gccaacttcg acaaaccaga gggaaagtgt ggcgatggtc tcgtgcgcgt
     2521 tgagaccgga cgggttgggt agcacccagg tctcggcccc ggcgagatcc cccttctgcc
     2581 ggcccaacat cgcctccgat tcaccgaagg cagtccggta cgcagtcaca ccggcgacgg
     2641 caaccaccga agggttcaag tctccaatgg tcgccctcag ccgctccccg cccacacgca
     2701 gctcatcgga gccgatctcc gaggcgcggg cggtggcccg ggcaaccagg ttggtgatcc
     2761 cgatgcctcg ggacagaaac tgcgctcgtt gggagtcgct catcccgaca tcggtgtcga
     2821 attcgatgtc gatgagaccg gcgaggcgaa gggccggata gaagcgattg gttggatggc
     2881 agaaatgggt ctgggtcgcc gcagtccaca gccccgggtt gatcccaacg aagaggagct
     2941 tgatgccggg ggccaccagg tcggggaccg tctgcccctc gtacgccttg agctgtgcct
     3001 gggtgaatcc cactggcgat tcaggcctcc gacgccggga gccagggtgg gatcggcgca
     3061 tcgattcgcc ccagcttcga aggaaccggg ccgaaccatt cctcgtcgtg cgtctgccag
     3121 gcccgccagg ccgccgtgat ctcgtccttc gagcgtgcga caaagttcca ccacatctca
     3181 atcggctctc cgagcggctc accacccaga agtaggaagc gcccgccatt aggaccgaca
     3241 tcgatcgtga tttggtcacc gcccggtggg accatcgcga gccaccccgg ctcgacgatg
     3301 tcctcaccca ccttcaggct gcgatcgatc ggcaccactg catattcgaa ggctgtctgc
     3361 agtgggacct cgaggaggcc gtgtcgggcc ttcacatcgg caccgaccat gagggtgtcg
     3421 acgtgggctg gtgactcgac cgctcccaat gtcccgacca tcaccgttgc ctcggcagtg
     3481 gcaaagtcga ccaccgggag ctccgcgacg tgttggaagc gagaggggcc gtgtcgggtc
     3541 tcttcgggtt gcgccaacca catctggacc ccgtgaatcc cggtcgtggt ccccaactcg
     3601 gcgtgggcga tcccatgccc ggcggtcatc aggttcagct gaccggggtg aatcagttgc
     3661 tcggtgccca gggagtcagt gtgaagggcg gagccttcga ggagccaggt gaccgtggcc
     3721 agattggtgt gagggtgggg gccgatctcc aagggatgcg gatcttcgaa gtcggggggg
     3781 aggatcagat caacgaagca ccagggccca acggtgcggc gacccttggt ggggaggatc
     3841 ctggtcacgg tggtggcacc gacagtcgcc gagcggccct cccggat
//
LOCUS       DBNHXU010000149         2312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1039779, whole genome shotgun sequence.
ACCESSION   DBNHXU010000149 DBNHXU010000000
VERSION     DBNHXU010000149.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1039779"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..779)
                     /locus_tag="AC161X_03845"
     CDS             complement(<1..779)
                     /locus_tag="AC161X_03845"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HIR4988711.1"
                     /translation="MKRLARAVAPVPVLVLLLALPAAAAPFVYPSPEFQNFPITGPPN
                     VSAPSWILYDASIDMVIASSGSTDERSIASTTKIMTGLLVVENADFEDVVTISQEAAD
                     TGGQEIGLVAGERMTVGSLFRALMVRSANDAATALAEHIAGSIDNFVQMMNQRAEEMG
                     LEHTHFANPHGMDAPGHYSSAQDLLTMAREAMSHPEFANVVRSKVIVMTPAPDGTTRV
                     GISTNLMLASYDGMMGVKTGLTPRALFTFVGAAEREGRELYT"
     gene            complement(776..1432)
                     /gene="ftsR"
                     /locus_tag="AC161X_03850"
     CDS             complement(776..1432)
                     /gene="ftsR"
                     /locus_tag="AC161X_03850"
                     /inference="COORDINATES: protein motif:HMM:NF050798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator FtsR"
                     /protein_id="HIR4988712.1"
                     /translation="MSIGEVINLLRDDFPDVSVSKIRFLESQGLIDPGRSDSGYRQFD
                     SRDLARLRFILQQQRDHFLPLKVIKSKLTLWERGEESVTSRHPEPRHNDDSAPLGRDD
                     VLKRSGLSSRQLDELVAAGVVRLIGESDVFPVEAGVVALEAKRLMDQGLEARHLRSIR
                     LSVDREADLLRQLTAPLMRAANSEARARARDVLELSAGSVQAMHRAMLEGELKQQTTQ
                     "
     gene            complement(1443..1883)
                     /locus_tag="AC161X_03855"
     CDS             complement(1443..1883)
                     /locus_tag="AC161X_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005963693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HIR4988713.1"
                     /translation="MTEDSQRAPGQPDPQVEVESDPTVVYVPIQDRGDHPLSREEASS
                     VEGFAGFALVIERGPQTGMTYVLAPGNTTVGRHPESDIFLNDVTISRQHCRFLLGEGK
                     LVVEDSGSTNGTYVNDARVDEAQLRAGDEVVVGRFHFLVAHGDA"
     gene            complement(1929..2309)
                     /gene="gcvH"
                     /locus_tag="AC161X_03860"
     CDS             complement(1929..2309)
                     /gene="gcvH"
                     /locus_tag="AC161X_03860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015485945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005960 - glycine cleavage complex
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HIR4988714.1"
                     /translation="MQIPDDLLYTEEHEWIRRVSDTTVRLGITDYAQDQLGDVVFVEL
                     PDVGATVAKDDILVEVESTKSVGEVYAPFSGKITAVNSAMVSAPELVNQSPYDEAWFV
                     EMEVGEAFGITSFLDASHYRELTE"
BASE COUNT          425 a          728 c          704 g          455 t
ORIGIN      
        1 gtgtagagct ctcgtccctc gcgttccgca gcgccgacaa atgtgaagag cgcacgagga
       61 gtgagcccgg tcttcacgcc catcatcccg tcgtaagagg cgagcatcag attggtgctg
      121 atcccaaccc gggttgtccc gtcaggggcc ggggtcatga caatcacctt cgacctcacc
      181 acgtttgcga actcaggatg gctcatcgcc tccctggcca ttgtcagcag gtcctgggcg
      241 ctggagtaat gacccggagc gtccatcccg tggggattgg caaaatgagt gtgctccaga
      301 cccatctctt ctgcccgctg gttcatcatc tggacgaagt tgtcgatgct gccggcaatg
      361 tgctcagcca gcgccgtggc ggcatcgttg gctgaacgca ccatcagcgc cctgaacagc
      421 gagccgacgg tcatccgctc cccggcgacg agcccgatct cctgtccgcc ggtgtctgcg
      481 gcctcctgtg agatagtgac cacgtcctca aagtcggcat tttcgaccac aagaagaccg
      541 gtcatgatct tggtcgtcga cgcaatcgac cgttcgtctg tggagcctga cgacgcaatc
      601 accatgtcga tggaggcgtc gtagagaatc cacgagggcg ccgagacgtt tggcggcccg
      661 gttatgggga agttctgaaa ctcgggggac gggtacacga atggagctgc tgccgccggg
      721 agggcgagca aaaggaccag caccggaacc ggtgccacgg cacgtgccaa tcgcttcatt
      781 gcgttgtttg ctgcttcagc tctccctcga gcattgcccg gtgcatggct tgcaccgaac
      841 ccgcactcag ctcgagtaca tcgcgggccc tggctctcgc ctccgagttg gccgcccgca
      901 tcaagggagc tgtcagctgt ctcaggagat cggcttccct gtcgactgac aggcgaatcg
      961 acctcagatg acgcgcctcg agaccctgat ccatcagacg cttggcttcg agggcgacca
     1021 ctcccgcttc gaccgggaac acgtccgact ctccgatgag ccgcaccacg ccggccgcca
     1081 ccaactcgtc cagctgtcgc gaggaaagcc cagaccgctt caggacatca tcccgcccca
     1141 gcggagccga gtcgtcgttg tggcgcggct ccggatgccg gctcgtcacc gactcctctc
     1201 ctcgctccca caacgtgagc ttggacttga tcaccttgag cggcaggaag tgatcgcgtt
     1261 gctgttgcag gatgaaccgc aatcgggcga gatctcggct gtcgaactgg cgatagccgc
     1321 tgtcggatcg cccgggatcg atcagcccct gggattcgag gaagcggatc ttggagacgg
     1381 acacgtcggg aaagtcatca cggaggaggt tgatcacctc gccgatgctc atcgaatcct
     1441 gttcaggcat cgccgtgtgc gacgagaaag tgaaaacgcc cgaccacgac ctcgtcgccg
     1501 gccctgagtt gggcttcgtc gactctggcg tcgttgacgt aggtcccgtt ggtggaaccg
     1561 ctgtcttcga ccacgagctt tccctcaccc aggaggaagc ggcaatgctg gcgggagatc
     1621 gtgacgtcat tgaggaagat atcgctctca ggatgtcggc cgacagtggt gttcccgggt
     1681 gccaacacat aagtcatccc ggtctggggc ccgcgctcga ttaccaaggc gaagcccgcg
     1741 aaaccttcga cgctgctggc ctcttcgcgg ctgagcggat ggtcgcctcg atcctggatc
     1801 gggacgtaga cgacggttgg atcgctctcg acctcaactt gagggtcggg ttggcccggg
     1861 gcgcgttggc tgtcctcggt catgaacgca gatgataggg agtgcccagc gttgcgccgg
     1921 gactcaagtc attccgtcag ttcgcggtaa tgactggcgt cgagaaagct cgtgataccg
     1981 aaagcctcgc cgacttccat ctccacaaac caggcttcgt cgtatgggct ttggttgacc
     2041 agttcggggg cgctcaccat ggccgagttg actgcggtga tcttgcccga aaagggggcg
     2101 taaacctccc ccaccgactt ggtgctctcc acctcgacca ggatgtcgtc cttggccacc
     2161 gttgccccga cgtcgggcaa ctccacgaag accacgtccc ccaactggtc ctgggcgtag
     2221 tcagtgatcc cgagtcgcac ggtggtgtcg ctgacgcggc gaatccactc gtgctcttcg
     2281 gtgtacagaa gatcatcggg tatctgcatc ag
//
LOCUS       DBNHXU010000150         1975 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3617535, whole genome shotgun sequence.
ACCESSION   DBNHXU010000150 DBNHXU010000000
VERSION     DBNHXU010000150.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1975)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1975
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3617535"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1300
                     /locus_tag="AC161X_03865"
     CDS             <1..1300
                     /locus_tag="AC161X_03865"
                     /inference="COORDINATES: protein motif:HMM:NF012609.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HIR4988715.1"
                     /translation="LDLAPGEAPRGVSGCWASAFTASAQALLAQSGVEPGAMRMAVLV
                     QPALEPDSGGLASIDGTGVVTVVAVKGPPGPLLQGWKAGTSAHYDGKWNGDELVEMLG
                     QTTLDRLVVAVVDANRTIGANYYEWAAVGGEVWLLQAGLAHRENNRTQLDTDPSYAED
                     ELVHAAWVIADFPGQLGAELILPWALGGLPESDQVSTGPPDLSLIASLCRDLTSEVWG
                     QPHDRARQIAIECLRSMRAGAPRRAVEQLQRLRPPNRKKAIHVLSMVAGLRSAVVEAG
                     AAPDPGGAWFMSVAEVQSALSGSGDVKAPLRVGAGVWEPFLFAVSRAAGTRMRGAPAS
                     DGFGAGLTWSPDKGKGAKGLHPRMVLAAPFPVPQIAPLLWDATGLVTSSGGVAAHLFE
                     SARALGVPAVSGIELPAGRGLAAVDGHSGLVSWLDRGSGD"
     gene            1293..>1975
                     /locus_tag="AC161X_03870"
     CDS             1293..>1975
                     /locus_tag="AC161X_03870"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015411.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR4988716.1"
                     /translation="MTERVGIIGLGVMGSGMANNLIRDGHQVIGFDVDPERIAVFEAG
                     GGRAATSAGEVAGNASLMILSLPTEDSLQQVAAEIAADAGPDLLCLETGTFTLEAKQS
                     ARSTLAAAGVELMDTPLSGTGLQAADGTLVVFASGSEAGYRRAAEVLASISKSTHFLG
                     EFGNGSKMKYVANLLVAIHNLATAEAHALGVAAGLEPSLVQAVMADGVGSSKIFEIRG
                     PMIVKDDYTP"
BASE COUNT          322 a          630 c          663 g          360 t
ORIGIN      
        1 tctcgatctc gcccccgggg aagcaccccg gggagtgtcc ggctgctggg cttcggcatt
       61 cactgcctcg gcccaagcac tactggcaca gtcgggggtc gagcctggag cgatgaggat
      121 ggcggtcctg gtccagcctg ccctggagcc ggactccggg ggcctcgcca gcatcgacgg
      181 aaccggtgtt gtcactgtgg tcgccgtcaa gggaccgcca gggcccttgc tgcaagggtg
      241 gaaagctggc acatctgcgc actacgacgg gaagtggaac ggggatgagc tcgtcgaaat
      301 gctcggtcaa acgactctgg acaggttggt cgttgcagtc gtcgacgcca accgcacgat
      361 cggggcgaac tactacgaat gggcggcagt cggtggagag gtctggctgc tccaggcagg
      421 gttggcccat cgcgagaaca atcggaccca gctcgacacc gatccgtcct acgccgagga
      481 cgaactggtt catgcagcat gggtgatcgc ggatttcccc ggccagctgg gagcggaact
      541 gatccttccc tgggccctgg gtggccttcc cgagtctgat caagtgtcga ccggcccgcc
      601 cgacctaagt ctgattgcga gcctttgtag agacctgacg tccgaagtct ggggccagcc
      661 tcatgatcgg gctcgacaaa tcgccatcga atgtctgcgc tcaatgcgtg cgggggcgcc
      721 tcgccgggcc gtcgagcagt tgcagcggct ccgccctccc aaccgaaaga aggcgataca
      781 tgtcctgtcg atggtcgccg ggttgcggtc tgcggtcgtc gaggctggag ccgcccccga
      841 ccccggaggc gcctggttca tgtctgtcgc cgaggtccag tccgctttgt cgggttccgg
      901 ggatgtcaag gccccacttc gggtgggtgc cggcgtctgg gagccgttcc ttttcgcggt
      961 gagccgagcc gcaggaaccc gcatgcgtgg tgcgccggca tctgatgggt tcggcgccgg
     1021 tctcacctgg tctccagaca agggcaaggg agccaaaggg cttcacccac gaatggtgtt
     1081 ggcggcacca ttcccggttc cccagatcgc cccgcttctc tgggatgcga ccggcctcgt
     1141 caccagctcc ggtggtgtcg ccgcccacct cttcgagtcg gcacgcgctc ttggagtccc
     1201 ggcggtgagt ggcattgagt tacccgccgg ccgcggcctc gccgcagtgg atggccatag
     1261 cggtttggtc tcgtggttgg atagggggag tggtgactga aagggttggc atcatcggtc
     1321 tcggggtcat gggatcggga atggccaaca acctgattcg ggacggccat caggtcatcg
     1381 gattcgacgt cgaccccgaa cgcatcgccg tcttcgaagc agggggcggc cgggccgcga
     1441 cttccgccgg ggaggtggcg ggcaacgcgt ctctgatgat cctttccctc cccacggagg
     1501 attcgttgca gcaagtcgcg gcggagatcg ccgccgacgc cggccccgac ctgttgtgtc
     1561 tggaaaccgg aaccttcacc ctcgaggcca agcagagcgc ccgctcgact ctcgccgcag
     1621 ccggcgtcga actgatggac accccactgt ctggcacggg cctgcaggcc gccgacggca
     1681 ccctggtcgt gttcgcatcg gggagcgaag ctggctaccg gcgagcggct gaagtcctgg
     1741 cgtcgatcag caaatccacc cacttcctag gcgagttcgg caacggctcg aagatgaagt
     1801 acgtcgccaa cctcttggtg gcgattcaca acctggcaac tgccgaggcc cacgccctag
     1861 gcgttgcagc cggtctcgaa ccttcgctcg ttcaggcggt catggccgac ggtgtcggtt
     1921 cctccaagat cttcgagatc cgcgggccga tgatcgtcaa agacgactac acgcc
//
LOCUS       DBNHXU010000151         3862 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_641372, whole genome shotgun sequence.
ACCESSION   DBNHXU010000151 DBNHXU010000000
VERSION     DBNHXU010000151.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_641372"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1048
                     /locus_tag="AC161X_03875"
     CDS             <1..1048
                     /locus_tag="AC161X_03875"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PLP-dependent cysteine synthase family protein"
                     /protein_id="HIR4988717.1"
                     /translation="TRTGTGCGFFVAQIEEMTMTTTTVATSLGTDREVRRYRDVRELL
                     PGADNPTPLVRLNHVVRPNQGEVYLKLEWLNPFGSIKDRAASYLLAGLVERGLLGERD
                     VVEATSGNTGIALAALAAVSGFGVTVTIPDGVPEEKKIILRMLGAEVWETPDDLCPVD
                     HPKDGAIALARSLAASEEGGRYVATDQYSNPDNVTAHYETTGPEIWHQTDGRVGWFVA
                     GLGTGGTITGVGRYLKERDPSIRIVGIEPQPGHRIPGLKSFQEAKEPEILDWDVIDDV
                     IKVDDGPAYEATRRLWREEALTVGPSTGAVVAAVQQLGTAETDVVVGISADSGLKYTS
                     YFAEILGDEGRPRV"
     gene            1065..1334
                     /locus_tag="AC161X_03880"
     CDS             1065..1334
                     /locus_tag="AC161X_03880"
                     /inference="COORDINATES: protein motif:HMM:NF013379.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097163 - sulfur carrier activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HIR4988718.1"
                     /translation="MTTQYEIPIEDLKVDAELDTSGLLCPLPVYKASIALGQLSKGQV
                     LHLISTDPGSLEDIPALARQLGDTLLGVERTESSQLFWIEKGDRP"
     gene            1331..1846
                     /locus_tag="AC161X_03885"
     CDS             1331..1846
                     /locus_tag="AC161X_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012310181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR4988719.1"
                     /translation="MTATLHSVAPVEETERAKPKRIALIASKGTLDQAYPPLILATTA
                     ASLGWEVGVFFTFFGLDILHQDRFDKLKVASLANPAGPVQLPNILGAMPGATAAATAM
                     MKKWMDRANMPSVPEFIDMSQGLGVRFFACSTTMGVLGVEESDLIKGAEIAGAAAFLD
                     YAAEADIQLFI"
     gene            complement(1843..2223)
                     /locus_tag="AC161X_03890"
     CDS             complement(1843..2223)
                     /locus_tag="AC161X_03890"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR4988720.1"
                     /translation="MEKVIAPGMARLPSFSHAVVAGDFIHVSGSLGTAEGTMELVPGG
                     TGPETTQVLANIRTILEASGAGLGDVVKMSIFLHDMSTFNEMNDAYLDVFPDSPPARI
                     TVGGVDLALGAAVEIDCVAYRPAG"
     gene            2275..3270
                     /locus_tag="AC161X_03895"
     CDS             2275..3270
                     /locus_tag="AC161X_03895"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR4988721.1"
                     /translation="MADRRRTGWGGEAVVAPGMDEIKLAAARLQGTAVRTPLVPLASY
                     DARADIFLKPEVLQPVGSFKVRGVGNWALSLTQEERGRGISTTSAGNTAAALGYMARR
                     LGVPARSMVPDSLHDTKRRMLASYGVELVHVSMDDLMGYMFEERWRDEPYSYLNPWGE
                     PLMIAGHATIGLEVFEDLPQIESVFVPVGGGALLCGVASALKALKPSIRVFAVQAAVN
                     SALSAAFAAGGPTWIEWHDTVVEGASTPVITDEMYPLLRSLVDEVVLVSEGQVKQAMR
                     QLALRDKLVTEGAGAAALAAALSVPVEQRGASVCVVSGGSVDETLLREVLAGQAG"
     gene            complement(3258..>3862)
                     /locus_tag="AC161X_03900"
     CDS             complement(3258..>3862)
                     /locus_tag="AC161X_03900"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4988722.1"
                     /translation="LAEGVENYSTEEITEMVHLIAGQSVEMAQLVDDLLTAERAASGN
                     LTIKPTAIDLLHETRSVVDSMRVGENMVVDGDPTPAWADTLRTRQIVRNLLTNASRYG
                     GNQVKVTVSSLGETAVMVVSDDDPGINVVDSERIFDPYYRVQNDDARPDSVGLGLAVA
                     RQLARIMGGDLVYRRRAGWTRFELTLPTTSETASALAYPA"
BASE COUNT          699 a         1187 c         1269 g          707 t
ORIGIN      
        1 gacccgcacc ggcacaggat gcgggttttt tgttgcccaa attgaggaaa tgacgatgac
       61 aacgacaaca gtcgccacct ccctcggcac cgaccgagag gtccggcgat atcgggatgt
      121 tcgggaattg ctcccgggag cggacaaccc cacccctctg gtgcgcctca accatgtcgt
      181 gcgcccgaac cagggtgagg tctatctgaa gctggaatgg ctcaacccgt tcgggtcgat
      241 caaggatcgg gcagccagct atctgttggc cggtctcgtc gagcgcggtc tgcttgggga
      301 gcgtgatgtg gtggaagcca cctccggtaa taccgggatc gctctcgccg ccctggccgc
      361 ggtcagcggt ttcggagtca cggtgaccat ccccgacggc gtccccgagg agaagaagat
      421 catcctccgc atgctcggcg ccgaggtgtg ggagaccccg gatgacttgt gcccggtcga
      481 tcatcccaag gacggggcta tcgccctcgc ccggtcgctc gctgcctccg aggagggcgg
      541 tcggtatgtc gcgaccgacc agtactccaa ccctgacaac gtcaccgccc actacgagac
      601 caccggcccc gagatatggc atcagaccga cggcagggtc ggctggttcg tcgccggtct
      661 cggcaccggc ggaacgatca ccggagtcgg gcgatatttg aaggagcggg atccatcgat
      721 ccggatcgtt ggaatcgagc cgcagcctgg tcatcgcatc cctggcctca agagcttcca
      781 ggaggcgaaa gagcctgaga tcctcgactg ggacgtgatc gacgacgtga tcaaggtcga
      841 cgacgggcct gcgtatgagg ccaccaggcg gctgtggcgc gaggaagcgc tgaccgtcgg
      901 tccatccacg ggcgcggtgg tggccgctgt ccagcaactc gggaccgcag agaccgatgt
      961 cgtggtgggg atctcggctg attcgggcct caaatacacg agctatttcg cggagatcct
     1021 cggtgacgag ggtcggccgc gtgtttgaca acaaggagga accagtgaca acccaatacg
     1081 aaataccgat cgaggacctg aaggtcgacg ccgagctcga cacttcaggc ctcctctgcc
     1141 cgttgcctgt ctataaggcg tcgatcgccc tcggccagct ctccaaggga caggtgctcc
     1201 acctgatcag caccgacccc gggtccctcg aggacatccc ggcgttggct cgccagctcg
     1261 gggacacgct gctcggcgtc gagcgaaccg agagcagcca attgttctgg atcgagaagg
     1321 gcgaccggcc atgaccgcga ccctgcatag cgtggctccg gtcgaggaga ccgagcgggc
     1381 caagccaaag cgaattgcgc tcatcgcgtc caaggggact ctcgaccagg cctacccgcc
     1441 actcatcctg gccacgactg cggcgagcct gggatgggag gtcggtgtct tcttcacctt
     1501 cttcgggctg gacatccttc atcaggatcg gttcgacaag ttgaaagttg cgtcgctggc
     1561 taacccagcg ggcccggttc aactcccgaa catcctcggt gccatgcctg gggccaccgc
     1621 ggctgccacc gcaatgatga agaagtggat ggatcgggcg aacatgcctt cggtccccga
     1681 gttcatcgat atgtcccagg gtctcggagt ccgattcttt gcctgctcca cgaccatggg
     1741 agtcctcggt gtcgaggaga gcgacctgat caaaggggcg gagattgctg gtgccgctgc
     1801 cttcctcgat tacgccgccg aggctgacat ccagctgttc atctagccgg ctggccgata
     1861 ggcgacacaa tcgatctcga ccgcggcgcc caaggccagg tcgaccccac caacggtgat
     1921 gcgcgccggt ggggagtcag ggaaaacgtc caggtaggcg tcgttcatct cattgaaggt
     1981 ggacatgtcg tggaggaaga tgctcatctt gaccacgtcg cccaaacctg ccccgctggc
     2041 ctcgagaatg gtccggatgt tggccagcac ttgggtggtc tccggcccgg tgccaccggg
     2101 caccagttcc atcgtcccct cggcggtccc cagggaaccc gagacgtgga tgaagtcgcc
     2161 ggcgaccact gcatggctga atgagggaag ccgggccatg cccggagcta tcaccttttc
     2221 catttgaaac ctcctggcaa atagagtcgg ctcgatgagt cgagggtcaa ggtagtggct
     2281 gatcgacgtc gcaccgggtg gggcggtgaa gcggtggtgg cgccgggcat ggacgagatc
     2341 aagctcgccg cggctcggct tcagggaacg gcagtgcgaa caccgctcgt cccgctagcg
     2401 agctacgacg cccgagccga catcttcctc aagcccgagg tcctgcaacc ggtcggctcg
     2461 ttcaaggtcc gcggggtcgg caattgggcg ctgagcctca ctcaagagga gcggggtcgt
     2521 gggatctcaa cgaccagcgc ggggaacacc gcggcagcgc tgggctatat ggcgaggcgg
     2581 ctgggagtcc cggcgaggtc gatggttccg gactcgctcc acgacaccaa gcggcgcatg
     2641 ctggcttcat acggggtgga gttggtccac gtgagcatgg atgacctgat gggctacatg
     2701 ttcgaggaac gctggcgtga tgagccctac agctacctca acccgtgggg cgagccattg
     2761 atgatcgctg ggcacgccac gattggtctt gaggttttcg aggacctccc acaaatcgag
     2821 tcggtattcg tgccggtcgg cggcggcgcg ttgttgtgcg gggtggccag tgcgctcaaa
     2881 gcactcaaac cctcaatccg tgtcttcgcg gtgcaggccg cggtgaactc ggcgttgtca
     2941 gctgcattcg ccgccggcgg tccgacatgg atcgaatggc acgacacggt ggtcgaaggg
     3001 gcttcgacac cggtgatcac cgacgagatg taccccttgc tccggtcgct ggtcgacgag
     3061 gtcgtcctgg tgtccgaggg tcaggtgaag caggcaatgc ggcagttggc tttgcgagac
     3121 aagctggtca cggagggcgc cggcgccgct gctcttgccg ccgccctctc cgtcccggtt
     3181 gagcagcgag gggcgagcgt ctgtgtcgtc agtggtggaa gtgtcgacga gacactgctt
     3241 cgcgaggtgc ttgccggtca ggctggatag gcgagcgccg aagccgtctc tgatgtcgtg
     3301 gggagtgtga gctcgaacct ggtccacccc gcccgtcgcc gatagacgag atcgccaccc
     3361 atgattcgcg ccagctggcg agccacggcg aggccgagcc cgaccgagtc aggccgcgca
     3421 tcatcgtttt ggacccggta gtacgggtcg aagatccgtt cggagtcgac aacattgatg
     3481 cccgggtcat cgtcactcac gaccatgacc gcggtttcgc ccaatgagga cacggtgacc
     3541 ttcacctgat tcccgccata acgggatgcg ttggtcaaca ggtttcgcac gatctgtcgg
     3601 gtgcgaaggg tgtcggccca agcaggcgtg gggtcaccgt cgaccaccat gttttcgccg
     3661 actctcatcg agtcgaccac cgatctcgtc tcatggagca agtcgattgc tgttggcttg
     3721 atggtcaggt tgcccgaggc cgcccgctcc gcggtcagga ggtcgtcaac gagctgggcc
     3781 atctcgacgc tctgacccgc gattaggtgg accatctcgg tgatctcctc agtggagtag
     3841 ttctcgaccc cttcggccag ct
//
LOCUS       DBNHXU010000152         3013 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4014680, whole genome shotgun sequence.
ACCESSION   DBNHXU010000152 DBNHXU010000000
VERSION     DBNHXU010000152.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4014680"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /gene="murI"
                     /locus_tag="AC161X_03905"
     CDS             complement(<1..658)
                     /gene="murI"
                     /locus_tag="AC161X_03905"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HIR4988723.1"
                     /translation="MKSMIGVFDSGVGGLGVLSHIRRELPDADLVYLADQARAPYGTR
                     SLQEVAAISMEVAEWLLERGATTVVVACNTASAAALSSLREIHPSVPFVGMEPAVKPA
                     ALVTRSGVIGVLATAATFQGELYSSVVARHAPDVQVLNAACPAWVRLVEAGMISGTEV
                     ERQVAECLAPLLEGGADVLVLGCTHFPFLRSVIEHVAGASVAVVDPAPAVALQVARVH
                     G"
     gene            716..1486
                     /locus_tag="AC161X_03910"
     CDS             716..1486
                     /locus_tag="AC161X_03910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011587941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR4988724.1"
                     /translation="MQVEGSVALVTGGVSGLGFGAATRLVGGGGRVVMLDLNEIRGPE
                     AVAELGENARFTLCDVRDPASVVAAVTGAVEEFGRIDILVNAAGVAPAQRVVARDGSL
                     FDLDLFRFVIDVNLVGMFDVIRNVAGAMAANEPGESAERGVIINVASIAAYEGQVGQA
                     AYSASKGGVVAMTLPLARDLAGIGVRVMTVAPGIMDTPLLDGAPEELRQALANIHVFP
                     KRLGTPADFAHLVQAIVENPLLNGEVIRLDAAARMPPR"
     gene            1497..2093
                     /locus_tag="AC161X_03915"
     CDS             1497..2093
                     /locus_tag="AC161X_03915"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019809710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HIR4988725.1"
                     /translation="MTTTTRIGLVCFDGMEELDLVGPWEVLRMWQLSWPDDGADVFTL
                     SLDGETVTCAKGMQIVTDRPMAEAGALEVIVYPGGRGTRRHIGDNRIGDWLRGLSEAG
                     TLITSVCTGSLVLADAGLLRNRPATTYWSNLENLSELDPTIELRPDDRFVDSGEVITA
                     AGVSAGIDMALHLVARLTSKERAREVKRAIQYDPHPPV"
     gene            complement(2106..>3013)
                     /locus_tag="AC161X_03920"
     CDS             complement(2106..>3013)
                     /locus_tag="AC161X_03920"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HIR4988726.1"
                     /translation="PGPDRIWQVASELELTFLGVSPTLVRALRIHGAEHAQRHDLSRL
                     RTFGSTGEPWNPEPWWWLFRDIGGESRPIINISGGTEIGAVLLSVNVHQGMKPTSLGG
                     PSLGVDADVYDSDGNPMRGEVGELVVRGSWPGRTRGFWNEPDRYLATYWERFDDTWVH
                     GDWASIDDDGFWFLHGRSDDTLNIAGKRIGPAEIESTAVAFPEVAMAAAIGIPDEIKG
                     EVIALYLVAAEGVTPDDSLSATIERAVVESLGKSFRPQTVRWVGDLPRTRSAKIMRRV
                     IKAIALGEEPGDVSSLENPESLKEI"
BASE COUNT          541 a          979 c          971 g          522 t
ORIGIN      
        1 cgccgtgaac ccgtgcgacc tgcagggcaa cagcaggagc cgggtcgacg actgcgaccg
       61 aggcgccggc gacatgctcg atcaccgagc gaagaaaggg aaagtgggtg cagcccagga
      121 ccaggacatc ggcaccgccc tcgaggaggg gcgccaggca ttcggcgacc tgtcgctcca
      181 cttctgtgcc gctgatcatc ccggcctcga ccaatcggac ccaggccgga caagcggcat
      241 tcagcacctg tacatcggga gcatgccggg cgacgaccga cgagtagagc tcgccctgga
      301 acgtggccgc ggtggccagt acgccgatca caccggatcg ggtgaccagg gctgccggct
      361 tcaccgcagg ctccattccc acgaagggca cggagggatg gatctcccgc agactgctga
      421 gggcggcagc tgaggcggtg ttgcaagcca cgacgacggt ggtggcgccg cgctcgagga
      481 gccattccgc gacctccatg gaaatcgcgg cgacctcctg gagggagcga gtcccgtagg
      541 gcgcccgagc ctgatcggcc agatagacga gatccgcgtc cggcagctca cgtcggatgt
      601 gggagagaac ccccagccct ccgaccccgg aatcgaacac accgatcatg ctcttcatgt
      661 cagcaggcca gagccccact cactggtagc gtcgcccgtc gtctctggag ggcacttgca
      721 ggtagaaggc tcggtcgcgt tggtcaccgg gggagtgtcg ggtctcggct tcggggcggc
      781 gacacggctc gtgggcggtg gaggccgggt cgtgatgctc gacctcaatg aaatccgggg
      841 cccagaagcc gttgcagagc tgggtgaaaa tgccaggttc accttgtgtg acgtacgcga
      901 cccggcatca gtcgtggcgg cggtgactgg ggcggtcgag gagttcggcc ggatcgacat
      961 ccttgtcaac gcggctggcg tggccccggc gcagcgcgtt gttgcccgcg acggttctct
     1021 attcgatctc gacctcttcc gattcgtgat cgacgtcaat ctcgtcggaa tgttcgacgt
     1081 catccgcaac gtggccggcg ccatggcggc caatgagccg ggcgagtccg ctgagcgggg
     1141 ggtgatcatc aacgtggcat cgatcgccgc gtatgagggc caggtgggcc aagccgctta
     1201 ttcggcatcc aaaggaggag ttgtggcgat gactctcccc ctagcccgcg atctcgctgg
     1261 catcggcgtc agagtgatga cggtggcgcc gggaatcatg gacacacctt tgctcgatgg
     1321 ggctcccgag gagcttcgcc aggcactcgc caacatccat gtcttcccca agcggctagg
     1381 cacaccagcc gacttcgccc atcttgtgca ggccatcgtt gagaacccat tgctgaacgg
     1441 cgaggtcatc cgcctcgacg ccgcggccag aatgccgcct cgataggagc cgagtaatga
     1501 ccacaactac gcgaatcggg ctcgtgtgct tcgacgggat ggaagagctg gatctggtcg
     1561 ggccttggga ggtgcttcga atgtggcagc tcagctggcc cgacgatggc gcagacgtct
     1621 tcactctgtc cctcgacggg gagaccgtga cctgtgccaa agggatgcag atcgtcaccg
     1681 atcggccgat ggccgaggca ggggcactcg aagtcatcgt ctatcccgga gggcggggca
     1741 cccgacgcca cattggcgac aaccgcatag gagattggct gcgaggtctg tccgaggctg
     1801 ggactctcat cacctcggtc tgcaccgggt cgttggtgct ggccgacgcc ggcctgctcc
     1861 gcaatcggcc cgccaccacc tactggtcga acctggagaa cttgtctgag ctcgacccga
     1921 ccatcgaact cagaccggac gaccgctttg tcgactcggg cgaggtgatc actgcggccg
     1981 gtgtgtccgc cgggatcgac atggcgctgc atctggtggc gcggctcact tcgaaggagc
     2041 gggcccgcga ggtgaagcgg gcgatccagt acgaccccca tccccccgtg tgatcacgcc
     2101 aaaccctaaa tctccttcag gctttccggg ttctccagac tcgagacatc accgggctcc
     2161 tcgcccaacg ctatcgcctt gatcacccgg cgcatgatct tcgccgagcg ggttcggggg
     2221 agatcgccca cccaacgaac tgtctggggt cggaagctct ttcccagact ctcgaccacc
     2281 gcccgctcga tcgttgcgct gagtgagtca tctggtgtca ccccttctgc agcgacgaga
     2341 tagagggcga tcacctcgcc cttgatctca tcgggaattc cgatcgcggc ggccatcgcc
     2401 acctcaggga aggcgaccgc ggtcgactcg atctcggccg ggccgattcg cttcccggcg
     2461 atgttcagag tgtcgtccga tctcccatgc aagaaccaga agccgtcatc gtcgatcgaa
     2521 gcccagtcgc cgtgcaccca ggtgtcgtca aatcgctccc aatacgtggc gaggtatcga
     2581 tcgggctcgt tccagaatcc acgcgtcctc cccggccacg agccacgcac caccagctcg
     2641 ccgacttcgc ctcgcatcgg gttgccatcg ctgtcgtaga catcggcatc gacgccaagg
     2701 gaggggccgc cgagtgatgt cggcttcatg ccctggtgga cattgaccga gagcagcaca
     2761 gctccgatct cggttcctcc cgagatgttg ataatgggcc tgctctcccc gccaatgtcc
     2821 ctgaacagcc accaccacgg ctccgggttc cagggctccc ctgtggagcc aaaggtccgc
     2881 agcctcgaca gatcatggcg ttgtgcatgt tcggcgccgt ggatgcggag cgctcgcacc
     2941 agggtcggtg agacgccgag aaaggtcagt tcgagttcgg aggcaacctg ccagatccga
     3001 tcaggccccg ggt
//
LOCUS       DBNHXU010000153         8661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4612046, whole genome shotgun sequence.
ACCESSION   DBNHXU010000153 DBNHXU010000000
VERSION     DBNHXU010000153.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8661
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4612046"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..305
                     /locus_tag="AC161X_03925"
     CDS             <1..305
                     /locus_tag="AC161X_03925"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP synthase subunit B family protein"
                     /protein_id="HIR4988727.1"
                     /translation="EREAFISRTNEKAREIVDRARNEADELVSDSSIVAEAVEEANLL
                     VRRAEGDARRLRLETEDEIEKKLQRVEGLLAEITTQVQTARDELHTARPAPPDVPI"
     gene            307..798
                     /locus_tag="AC161X_03930"
     CDS             307..798
                     /locus_tag="AC161X_03930"
                     /inference="COORDINATES: protein motif:HMM:NF014657.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceD family protein"
                     /protein_id="HIR4988728.1"
                     /translation="MTSDLKVPIHDLIGAPGKQRPFAGEQAVQLRLGESIIDGPMAVE
                     GVVTGLIEAVQAHFTVSGLAHLTCTRCLTEWEETITADGEQVYRRVADEDGYGIVDDE
                     VDISGPAQDELALSLPIVPLCKPECKGLCPTCGTDLNTEPCDGHGEDPDSPFAVLRDL
                     FDS"
     gene            820..996
                     /gene="rpmF"
                     /locus_tag="AC161X_03935"
     CDS             820..996
                     /gene="rpmF"
                     /locus_tag="AC161X_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015934 - large ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HIR4988729.1"
                     /translation="MAVPKKKMSRSRTRQRKAAWKVSRPTAVACDQCGKPKLPHRACR
                     ECGTYKGREVVKAD"
     gene            1000..1971
                     /gene="plsX"
                     /locus_tag="AC161X_03940"
     CDS             1000..1971
                     /gene="plsX"
                     /locus_tag="AC161X_03940"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018084856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HIR4988730.1"
                     /translation="MARIALDAMGGDNAPTETVAGAVEASQRGVDVVLVGDHLALEME
                     LAKYDARLPIVDAPEVIGMSDDPARSLREKPGASITVAAKLVAEGTADGLVSAGSTGA
                     SMAAAAIIIGRIKGVSRPSIASLFPTPGSPTLVLDSGANPDVKPEQLAQFGIMGSVAV
                     QVMLGLDHPRVGLLSNGEEKGKGRDLERAAYDLLDSSNINFIGNVEGRDLATDRADVI
                     VTDGFTGNIVVKTSEGAISIVALYLLEELSALDPAIQEAAMPALFRVRQRLDWETYGG
                     AQLLGVKGVVVIAHGASSRVAIANALVLAGDSADQGLPGRLAVELGS"
     gene            1968..2645
                     /gene="rnc"
                     /locus_tag="AC161X_03945"
     CDS             1968..2645
                     /gene="rnc"
                     /locus_tag="AC161X_03945"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019876414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HIR4988731.1"
                     /translation="MIPLEDELGYVFVNPDLLHLSLTHRSVSADDLTRIDNERLEFLG
                     DAVLQLVVTDRLYLEYPDFAEGQMAKIRSALVSRPTLADVARAIGLGGHIELATAEER
                     SGGREKDSILADAMEAVIGAVYLDGGIEKATLLIDRLWGDRVGDRAMSPGIKDYKTRL
                     QEVLAKDGRRPEYGSHGSGPDHEKVFEAWVEVAGAEIGRGSGRSKKEAEQEAARLAIE
                     TLSSGSI"
     gene            complement(2626..3432)
                     /locus_tag="AC161X_03950"
     CDS             complement(2626..3432)
                     /locus_tag="AC161X_03950"
                     /inference="COORDINATES: protein motif:HMM:NF051612.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF7847 domain-containing protein"
                     /protein_id="HIR4988732.1"
                     /translation="MTTRPRGAGDVLTSGISMLAKVWRSLVAPSVGAYILLGALTLVT
                     FQMTGANEFLTLSFNDPQVLEDMAAEEFLEVSTPFVQALLIGLVFQILASAFISLAAS
                     RVVGSEIEGERISGGSASRFALSRMLTMTVIGLITAVAVSVGLVLLVLPGVWLAVSVS
                     LAPQIVALEDRGVIASITRSIKLVRGRWWGTFGFLLLVGLVGSVAGWLLQSVAVPLVA
                     IGDVSTGLALVFVAGLIFQGLVVAAIAVVITMWYLELAARDETEVRLTPS"
     gene            3475..4314
                     /gene="mutM"
                     /locus_tag="AC161X_03955"
     CDS             3475..4314
                     /gene="mutM"
                     /locus_tag="AC161X_03955"
                     /EC_number="3.2.2.23"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003906 - DNA-(apurinic or apyrimidinic
                     site) endonuclease activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-formamidopyrimidine
                     glycosylase/DNA-(apurinic or apyrimidinic site) lyase"
                     /protein_id="HIR4988733.1"
                     /translation="MPELPEVEITRRHLNEAMGGHAFTAVTVSHARTARHNGDPIEVE
                     QRLVGRRVESVGRKGKFLVVPLDDGQTMVAHLGMSGRFAVESEDAPLAAHTHFRGLLD
                     DGTEIRFVDPRTFGFVAVFTEEELETSGVARLGPDAWLDLPTTEELSTALSRRTAPIK
                     ALLLDQGPISGLGNIYADEALHIARIHPLRPGNSLSSEDLERLRGAVGQVLEMAIENG
                     GTSLDDLAYLLPDGSAGENLDRLLVYGRADLPCGNCGTPIERIVIRARSSHFCPQCQP
                     IAP"
     gene            4311..4607
                     /locus_tag="AC161X_03960"
     CDS             4311..4607
                     /locus_tag="AC161X_03960"
                     /EC_number="3.6.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010469974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003998 - acylphosphatase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HIR4988734.1"
                     /translation="MTLKTVRAVVSGRVQQVGFRQSCRQMARSLGLVGWVQNLDDGRV
                     EVLAQGESEQLDRLVSWLWTGPAGAWVTGVESEAVSGDQTLRDFFIHADPSKSR"
     gene            4670..8119
                     /gene="smc"
                     /locus_tag="AC161X_03965"
     CDS             4670..8119
                     /gene="smc"
                     /locus_tag="AC161X_03965"
                     /inference="COORDINATES: protein motif:HMM:TIGR02168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HIR4988735.1"
                     /translation="MYLKSLKLVGFKSFADRTRLEFRPGVTVIVGPNGSGKSNLVDAL
                     SWVLGTQSPKTLRTGKMEDVVFAGTATRPALNRAEAVLVIDNAERQLPLDLDEVAIAR
                     RLYRDGSSDYEINGVGCRLLDIQELLSDSGVGRHQHVIIGQGQVDSVLNASPEDHRAV
                     IEEAAGILKHRRRKEKAERRLERTDEDVLRLQDLLGEIERQLRPLRRQARAAASYEGL
                     RDEVRSLRLYLSGAAIRELDDHLLMAKDEHDKLGETVAVDGRDVVVIGERVAELTARA
                     SQLGGEVERDTGAAALLETTAERLRRTASVAHERSRAASGRFQATAERRADLEAELYA
                     IRGEMEGLETAAIEANNDADSREIRFRRLEDEERVIATQNALSAEGAVAAVRGELSIL
                     EAAGLRDDRELEAISHRLDVVDGQLRDESNEISILNEEVRAIDEKVVKLQAVFEQTTR
                     RRSGDQEKWSTAEEGLIDARLAVAAAKARVEAIGAALAGRFDREARRSVEGADGSRGT
                     LISLLDVPTGLEAAVDAALGRWADAVAFDGSASMQHAVETVKASGGGGVPVVAAATAE
                     LAPAKSVAASAGVEALIDRLGQKADPGLANHLLGDVLLVEGWTTAWSMISRHPDLRAV
                     TPEGDLISSRGVQIASPDGAGPAMLESAEVDLESAETDLSRAVSIHTAAKRDFDKSRD
                     HERVALEELESVESDLSGRTDAMARLQKSVNVLDGERSRLAQRRNSVVEGVSDRERQV
                     VQLQQRLASLEGEEAERMRVWEELEASRARIAVDKELARSEWQESATTLQSIVERQRL
                     LQERLTHIESDMGRVEAVPSSTISAATLDQIEMFARRGVEVLQTRLNEVRQRQADLRA
                     SHLVTVTDLEQARAHHEELRDRIASSRERLSELQVLLTEFRLRRESLVEGVRRDADAE
                     VDAALEAERPDLPEGTDLEGLLEERIAQLRRLGPINPLASVEFAELDERHTFLTDQLA
                     DVESSRAELRKVIRALDEEIESRFQEAFQEVAEAYQRYFTVLFPGGRGRIRLTDASDP
                     ESGLMIEAQPLGKKVSQLTLLSGGERSLAALAFLFSVFEARPSPFYVLDEVEAALDDA
                     NLRRFLRIVDEFRQRAQLLIVTHQQQTMEAADVLYGVTMEPGGSSQAVRKDMTMVGAD
                     VFA"
     gene            8139..>8661
                     /locus_tag="AC161X_03970"
     CDS             8139..>8661
                     /locus_tag="AC161X_03970"
                     /inference="COORDINATES: protein motif:HMM:NF014880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle receptor subunit
                     alpha"
                     /protein_id="HIR4988736.1"
                     /translation="MDTTALVLTVLAVTLVIAVVVVVVVLRNRTVPIPGEAAPPSGAT
                     AQDSLGSSLRKVFRSGGVSEQTWADLEEAMLAADVGVDATAAVVKGVRSAHPETADEA
                     REQVARQLRIELGDKPRDLALAGKPAVILVVGVNGTGKTTTIAKIAARLKDEGKKVIL
                     AAADTFRAAAGAQL"
BASE COUNT         1592 a         2710 c         2874 g         1485 t
ORIGIN      
        1 gggagcgtga agcgttcatc tcccgcacca acgagaaggc ccgggagatc gtggaccggg
       61 cccgcaacga agccgacgaa ttggtctcgg actccagcat cgttgccgag gccgtcgagg
      121 aggccaacct cctggtgcga cgcgccgaag gcgatgcccg ccgtctccgg ctcgagaccg
      181 aggacgagat cgagaagaag ttgcagcggg ttgaaggtct cctcgccgag ataacgaccc
      241 aggtgcagac ggcccgggat gagttgcaca ctgctcgccc ggcgccaccc gacgttccga
      301 tctgagatga catccgacct caaggtcccg atccacgatt tgatcggagc ccccggcaag
      361 caacgtccgt tcgcaggcga gcaagccgtg cagcttcgcc tcggcgagtc gatcatcgat
      421 gggccgatgg cggtcgaggg tgtggtcact ggcctcatcg aggccgtcca ggcacatttc
      481 accgtgtcgg gcctcgccca tctcacctgc accagatgcc tcaccgagtg ggaagaaacc
      541 atcactgcgg acggtgagca ggtctaccgc cgggtggccg acgaggacgg ttacggcatc
      601 gtcgacgacg aagtcgacat ctcagggccg gctcaagacg agctggcact gagtcttccc
      661 atcgttccgt tgtgcaaacc ggagtgcaag ggactttgcc caacgtgtgg aaccgacttg
      721 aatacggaac cctgcgacgg tcatggggag gatcccgatt cacctttcgc cgtcctgaga
      781 gacctgttcg attcctgacc tatcaaggag tgcccgcaga tggcggtgcc caagaagaag
      841 atgtcgcgct cgcggacccg gcagcgcaag gctgcctgga aggtgtcccg gccgactgcc
      901 gtcgcctgtg atcaatgtgg caagcccaag cttccgcatc gtgcctgtcg tgaatgcggc
      961 acctacaagg gccgcgaggt cgtaaaggca gactgaggct tggcacgcat cgccctcgac
     1021 gcaatggggg gcgacaacgc cccaactgag actgtcgctg gtgccgttga ggcttcccaa
     1081 cgtggcgtag acgttgtact cgtcggcgac cacctcgctc tcgagatgga gctggcgaaa
     1141 tacgacgccc ggcttccgat agtcgacgct cccgaggtca tcgggatgtc cgacgatccg
     1201 gcgcgctcgc tccgtgagaa gccgggagcc tcgatcaccg ttgctgccaa gctggtagcc
     1261 gaagggactg ccgacggcct ggtctcggcc ggatccaccg gagcatcgat ggcggcggcg
     1321 gcaatcatca tcgggcggat caagggtgta tcccgcccct cgatcgcctc actcttcccg
     1381 acaccgggct cgccgacgtt ggtgctcgac tcaggcgcca acccggatgt aaagcccgag
     1441 cagctggccc aattcggaat catgggctcg gtcgcggttc aggtgatgct ggggctggac
     1501 catccgaggg ttgggctcct ctccaacggt gaggagaagg gcaagggtcg tgacctggag
     1561 cgtgccgcat acgacctcct cgatagctcc aacatcaact tcatcgggaa cgtcgagggc
     1621 cgcgacctcg ccaccgaccg cgccgatgtg atcgtcaccg atggattcac cggcaacatc
     1681 gtcgtcaaga ccagcgaggg cgcgatttcg atagttgccc tctacctcct cgaggaattg
     1741 tcggcactcg atccggcgat acaggaggcg gcgatgcccg ccctgttccg ggtgcggcag
     1801 cgtctcgatt gggagacata cggcggggcc cagctgctcg gagtcaaagg cgtggtcgtc
     1861 atcgcccacg gtgcgtcgag ccgggtggcc atcgccaacg ccctcgtcct ggccggggac
     1921 agcgctgatc agggcttgcc ggggcgtctc gccgtcgagt tgggctcgtg atccctctcg
     1981 aggacgagct cggttacgtc ttcgtcaacc ccgacctgct ccatctgtcc ctgactcacc
     2041 gctcggtcag tgcggacgat ctcacccgca tcgacaacga acggctcgag tttctcggtg
     2101 acgctgtcct ccagctggtg gtcaccgatc ggctgtacct ggaatacccc gactttgcgg
     2161 aaggccagat ggcgaagatc cgatctgccc tggtcagtcg tcccacgctg gccgacgtgg
     2221 cgcgggccat cggtctgggt ggccacatcg agctggccac cgctgaggag cgatcgggag
     2281 gtcgggagaa ggattcgatc ctggccgatg caatggaggc cgtcatcggc gcggtctatc
     2341 tagacggcgg gatcgagaag gcgacgttgc tcatcgaccg cttgtgggga gatcgggtgg
     2401 gcgatcgggc gatgagcccg ggaatcaagg actacaagac tcggctccag gaagtcctgg
     2461 ctaaggacgg tcggaggccg gagtacggca gccacggatc agggcctgac cacgagaagg
     2521 tgtttgaggc ctgggtcgag gtggccggcg ctgagatagg ccgggggagc ggccgctcca
     2581 agaaagaggc ggaacaggaa gcggcccgtc tcgcgatcga gaccctcagc tcggggtcaa
     2641 tctgacctct gtctcgtcgc gagcggccag ctcgaggtac cacatcgtga tcacgacggc
     2701 gatcgccgcc accacgagac cctggaagat cagcccggcg acaaacacca gggcgagacc
     2761 cgtcgagacg tcaccgatcg cgaccagggg aacggccact gattgcagaa gccatccggc
     2821 gaccgatccg acgaggccga ccagcagcaa gaagccgaac gtcccccacc agcgtcccct
     2881 caccagtttg atgctccgtg tgatcgaggc gattacccca cgatcctcca gtgccacgat
     2941 ctgaggagcc agcgacaccg agaccgcgag ccacacgccc gggagcacca gcagcaccag
     3001 accaacggag acggcaaccg cagtgatcaa cccgatcacg gtcatggtca gcattcgact
     3061 gagcgcaaag cggcttgcgc tgccacccga gatccgctcg ccctcgatct cggatccgac
     3121 gacccggctg gcggccaggc tgatgaacgc cgaggcaagg atctgaaaga caagcccaat
     3181 caggagcgct tgcacgaacg gcgtcgatac ttcgaggaac tcctcggccg ccatgtcctc
     3241 caggacctga gggtcgttga aactgagggt gaggaactcg ttcgcccccg tcatctgaaa
     3301 cgtcacgagg gtcagggcgc cgaggaggat gtaggcgccc accgaaggcg ccacaaggct
     3361 tcgccatacc ttcgccagca tgctgatccc ggaagtcagg acatcgccgg cgccgcgtgg
     3421 tcttgtggtc attcgacggg gtacggtaac ccgccctggt tgcggagtag atgcgtgccc
     3481 gagctgcccg aagtcgagat cacacgacgt cacctgaacg aggcgatggg cgggcatgcc
     3541 ttcacggcag tgacggtgtc tcacgctcgg accgcccgtc acaacgggga cccgatcgag
     3601 gtcgagcaac gacttgtcgg gcgccgcgtc gagtcggttg gtcgcaaggg caaattcctg
     3661 gtggtaccgc tcgatgacgg gcagacgatg gtggcccacc tcgggatgtc ggggcgattc
     3721 gccgtggagt ccgaggatgc tccgttggcg gctcataccc acttccgggg tttgctcgac
     3781 gatggcaccg agattcgatt cgtcgacccg cgcacctttg gattcgtcgc agtgttcacc
     3841 gaggaggagt tggagacctc aggcgtcgcc cggctcgggc ctgatgcctg gctggacctt
     3901 ccaaccacgg aggaactgtc cacagcattg tccaggcgaa ccgcgccgat caaggccctt
     3961 ctgctcgatc agggcccgat ctcaggcctc ggcaacattt atgccgatga ggctcttcac
     4021 atcgcccgga ttcacccgct acgccccggg aattccctgt cgtccgaaga tctcgagcgg
     4081 cttcgcggcg cggtcggcca ggttctcgag atggcaatcg aaaacggagg caccagtctc
     4141 gatgaccttg cctatctact gccggatggg agcgcagggg agaacctcga ccggctcctt
     4201 gtctacggcc gggcggacct tccgtgtggc aattgcggca ccccgatcga gcgcatcgtc
     4261 atccgtgccc ggtccagcca tttctgcccc caatgtcagc cgattgcccc atgactctga
     4321 agacggttcg cgcagtcgtc agcggacggg tgcagcaggt ggggtttcgg cagagttgtc
     4381 gtcagatggc gcgatcactt ggcctcgtgg gctgggtcca aaacctcgat gacgggcggg
     4441 tcgaagtgct cgcccaggga gagtccgagc aactcgaccg tttggtcagc tggctctgga
     4501 ctggcccggc tggagcctgg gtgactgggg tggagtcgga ggcggtgtcc ggtgatcaga
     4561 ccctccgaga cttcttcatc cacgccgatc ccagtaagag ccgctgaaca gcacatcgct
     4621 gttagcctga ctcgataatc acagtcgtgt catttgccag gaaatcactg tgtatttgaa
     4681 gtcactcaag cttgtaggtt tcaagtcctt cgccgaccgg actcgtctcg agttccggcc
     4741 cggggtcacc gtgatcgtgg ggccgaacgg ctccgggaag tcgaacctgg ttgacgcgct
     4801 ctcttgggtc ctaggcaccc aatcgcccaa gacgctccgc accggcaaga tggaagacgt
     4861 ggtgttcgcc ggcaccgcca cccgcccagc cctcaatcgg gccgaggcgg tgttggtcat
     4921 cgacaatgcg gagcggcagc ttcccctcga cctcgacgag gtcgccatcg ctcgccgtct
     4981 ctaccgggat gggtcgagtg actacgagat caacggcgtg gggtgtcgcc tgctcgacat
     5041 ccaggagctt ctgagcgact cgggtgtagg tcgccatcag catgtcatca tcggccaggg
     5101 ccaggtcgac tcggtcctca atgcctcgcc tgaggaccat cgggcggtga tcgaggaggc
     5161 tgccggcatc ctcaagcacc gtcgtcgcaa ggagaaggcc gagagaaggc tggaacggac
     5221 cgacgaagac gtcctccgcc ttcaggacct tctcggcgag atcgagcgtc aactccggcc
     5281 gttacggcgg caggcgcgtg cggcagcgag ttatgaaggg ctccgagacg aagtccgttc
     5341 ccttcggctc tacctgtccg gtgccgccat ccgagagctc gacgatcacc tactcatggc
     5401 caaggatgag cacgacaagc tcggtgagac cgttgccgtc gatggccgag atgtggtggt
     5461 gatcggcgag cgggtggccg agctcacggc tcgcgcctca caacttggcg gcgaggtcga
     5521 gcgggacact ggcgccgcgg ccctgctgga aaccactgcc gagaggttgc gccggaccgc
     5581 gtccgttgcg cacgagcgga gccgggcggc atccgggaga tttcaggcga cggccgagcg
     5641 cagggccgac ctcgaggccg agctgtacgc gatccgtggc gagatggagg ggttggagac
     5701 ggcggccatc gaggccaaca acgacgccga ctcccgggag attcggttcc ggcggttgga
     5761 ggacgaggaa agggtcatcg ccacccagaa tgccctttct gcggagggag ccgtagccgc
     5821 ggttcggggc gaattgagca ttctcgaagc cgccggcttg cgcgacgacc gtgaattgga
     5881 ggccatctcg catcggctgg atgtggtcga tgggcagctt cgggacgaat ccaacgagat
     5941 ctcgattctc aacgaggaag tgcgagcgat cgacgagaag gtcgtcaagc tccaggccgt
     6001 cttcgagcag acgacccgtc gtcgttccgg tgatcaagag aaatggtcaa cagccgagga
     6061 gggcctcatc gatgcccggc ttgcggtggc cgctgccaaa gcgagagtcg aggcgatcgg
     6121 ggccgctctc gccggtcgct tcgatcggga ggcccggcgc tcggtcgaag gagcggatgg
     6181 gtcgagggga accctgattt cgctgctcga tgttccgaca ggtctcgaag cagcggttga
     6241 cgctgccttg ggacgttggg ccgacgcggt ggccttcgat ggctcggcgt cgatgcagca
     6301 tgcggtggag actgtcaagg cctccggtgg aggcggtgtg ccggtggttg cggcggccac
     6361 agctgagctg gctccggcga agtcggtcgc cgcgagcgcg ggggttgaag ctctcatcga
     6421 tcgactcggg cagaaggcgg accccggctt ggccaaccat ttgctcggag acgtcctctt
     6481 ggtcgagggc tggaccacgg cttggtccat gatctcgcgc cacccggacc tgcgagccgt
     6541 cactcccgaa ggtgacctca tttcatcgag aggggtccaa atcgcctcgc ctgacggcgc
     6601 cggcccggcg atgctcgagt ccgctgaggt ggatctcgag tcagcggaga ccgatctctc
     6661 gagggcggtc tctatacaca ccgccgccaa acgggacttc gacaagagcc gggatcacga
     6721 gcgggtcgcg ctcgaggagc tggaatcggt tgaatccgac ctctcagggc gcaccgacgc
     6781 catggcccgg ctccagaagt ctgtgaatgt cctcgacggc gagcgctcac gccttgctca
     6841 gcgccgaaac tcggtggttg aaggtgtcag cgatcgggag cgtcaggtcg tccaattgca
     6901 gcaacggctt gcgtctctcg agggcgaaga agccgagcgg atgcgggtgt gggaggaact
     6961 ggaggcgagc agggcacgga tcgcggtcga caaggagctc gcccgctcgg aatggcagga
     7021 atcggccacg acacttcaat ccatcgtcga gcggcaacga cttctccagg agcgcctcac
     7081 ccacatcgag tcggatatgg gccgggtgga ggccgtgccg tcgagcacca ttagtgccgc
     7141 caccctcgat cagattgaga tgttcgcgcg acggggggtc gaggtcctgc agacacgact
     7201 gaatgaggtg cgtcaacgcc aggccgacct tcgagcatcc catctggtga cagtgaccga
     7261 tctggaacag gctcgagccc accacgagga gcttcgggac cggatcgcca gctctcgcga
     7321 gcgcctcagc gagcttcagg tgcttctcac cgagttccgt ctccgacggg agagcctcgt
     7381 ggagggggtc cggcgagacg ccgacgcaga ggtcgacgcg gcgctggagg cggagcggcc
     7441 cgacttgccg gaaggcaccg atctcgaggg cctgctggag gagaggatcg cccaactacg
     7501 ccgcctcggc ccgatcaatc cattggcgtc ggtcgaattc gccgagttgg atgagcggca
     7561 caccttcctc accgaccagc tcgccgatgt cgagtcgtct cgggccgagc tcagaaaggt
     7621 catccgtgcc ctcgacgagg agatcgagag ccgcttccag gaggcgttcc aggaagtcgc
     7681 cgaggcctat cagcggtact tcaccgtgct cttccccggt gggcgggggc ggatccggct
     7741 caccgatgcg tcggatcccg aatcggggct catgatcgag gcccagccgc ttgggaagaa
     7801 ggtcagccaa ttgaccttgc tctctggtgg ggagcgcagc ctcgccgcgc ttgccttcct
     7861 gttctcggta ttcgaagccc ggcccagccc cttctatgtc ctcgatgagg tggaagccgc
     7921 cctggacgac gccaacttga ggcgcttcct gagaatcgtc gacgagtttc gtcagcgcgc
     7981 tcaactcttg atcgtcaccc accagcagca gaccatggag gcggctgatg ttctatacgg
     8041 cgtcaccatg gaaccgggag gctcctcgca ggcggtccgc aaagacatga cgatggtcgg
     8101 cgccgacgtc ttcgcctagc ggtaacttca ctttctccat ggacaccaca gccctcgtcc
     8161 tgaccgtgct cgcggtcacc ctggtgatcg ctgtcgtcgt ggtggtggtg gttctccgga
     8221 atcgcaccgt tcccatcccg ggtgaggccg ctccgccgtc tggcgctact gcacaggact
     8281 ctctgggctc atccctgcga aaggtcttcc gatcgggtgg ggtgagcgag cagacctggg
     8341 ccgacctcga agaggcgatg ttggccgccg acgttggtgt cgatgccacg gcggccgtcg
     8401 tgaaaggcgt tcgctcggca catcccgaga ccgccgatga agcgcgggag caagtggcgc
     8461 gccagcttcg tatcgagctt ggagacaagc cacgtgactt ggctctggcc gggaagcctg
     8521 cggtgatcct ggttgtcgga gtcaatggca cgggcaagac caccaccatc gccaagatcg
     8581 cggcccggct caaggatgaa ggcaagaagg tgatcctcgc tgcggccgac accttccgtg
     8641 ctgcggccgg agcccaactc c
//
LOCUS       DBNHXU010000154         3157 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1239780, whole genome shotgun sequence.
ACCESSION   DBNHXU010000154 DBNHXU010000000
VERSION     DBNHXU010000154.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1239780"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..677
                     /locus_tag="AC161X_03975"
     CDS             <1..677
                     /locus_tag="AC161X_03975"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR4988737.1"
                     /translation="YVLALRAIWGAWQNSTPLKYEGEFYNLSLMTPFFDPGPIPNPDI
                     PVYIAGVGPYLSRLAGELCDGLHVHPFHTTRYLDRVVLPGITEGATRASRSLDDVELA
                     TTVFVMTGETDAEIETAMSGVRQQISFYASTPTYKPVLESNDWDFGEQLTALSKKGQW
                     GEMAAVVPDEAVLEVGVAAPIDRLGTAIRERYGDRVQRIGFYAIGSGLDSDREALTEV
                     IRQIRL"
     gene            complement(694..2334)
                     /locus_tag="AC161X_03980"
     CDS             complement(694..2334)
                     /locus_tag="AC161X_03980"
                     /inference="COORDINATES: protein motif:HMM:NF013206.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase family protein"
                     /protein_id="HIR4988738.1"
                     /translation="MRLRGGRGGQGRGESRARRPEPFPPRPQTVLTHAVATPHYLSAE
                     AARRVLLAGGNAVDAAIAATAAQGVVAPETCGIGGDLFALIYRPGWHRPLALNASGRA
                     GSNADPEVLRSLGGAEIPRDHPLTVTVPGCVDGWQALSAELGVLGLADCLQPAIELAE
                     GGFAVSTEQAKAFRQTEAMYSTHPAVHEFYPDGDVVSAGDIVQRIPLAVTLRAVARDG
                     RDAFYRGEPAVEIVNELDGTITLDDLARSHADWVPPISCDVTGVSAWTIPPNSQGYLG
                     PATLAVFEMLDPPDDPEDPDWLHLLIEAFRAVAWERDDIVADPETAPLPADLLLNHDR
                     LRRAAATVHREQTGIWPRSMGTESSTAYLCVVDAEGTAVSIIHSNYSGTGSAFGAPRG
                     GFLLHNRGGGFTLTPGHPNELMPHKRPLHTLSPTLWTDDGQSRWVIGTRGGSVQPQLV
                     AQVAARAVIAGQDLETAQAAPRWTIADHGPYSAPRSAIEPGMPSSTLAELRRRGHRIE
                     ELPERQPGWGPVSIIEVDGAELRAAADPRVDTTSALVF"
     gene            complement(2241..2861)
                     /locus_tag="AC161X_03985"
     CDS             complement(2241..2861)
                     /locus_tag="AC161X_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988739.1"
                     /translation="MDIVAFQEKIGRIDGVEAARVVYGNGHISEIHVLARRNKPPKQL
                     VRDVQSLSQALYGQEIDRRVVSVVQLADGDLEGGFRPALIDVAETLEGSRAEVTVSLR
                     WENEILSGTSTGAAAASTRARQIAEATLDAVRQGIHKEAALAINSMDVPLLGNRKVAV
                     AQVVVVTEASERMLIGCAYVEDEEGRAVVRAVLDALNRFLPDLKLS"
BASE COUNT          544 a         1000 c         1054 g          559 t
ORIGIN      
        1 actacgtcct ggcgttgcga gccatctggg gagcgtggca gaactcgacg cctctcaagt
       61 acgaggggga gttctacaac ctttcattga tgactccgtt cttcgatccc gggcccattc
      121 ccaatccgga tatcccggtc tacatcgccg gtgtcggtcc ctatctgagc cggctggcgg
      181 gcgagttgtg cgacgggctt cacgtccacc ccttccacac cacccggtat ctggatcggg
      241 tcgttctacc cgggatcacc gaaggggcga ctcgggccag tcgctcgctg gatgatgtcg
      301 agctggcgac aactgtgttc gtgatgaccg gggagaccga tgccgagatc gagacggcga
      361 tgagtggggt tcgacagcag atctccttct atgcctcgac ccctacctac aaaccggtgc
      421 tggagtcgaa cgactgggac ttcggcgagc aactgaccgc gctgtccaag aagggccaat
      481 ggggggagat ggcggcggtg gtacctgatg aggctgtgct cgaggtaggg gtggctgccc
      541 cgatcgaccg cctcggcact gcgatcaggg agcgctacgg cgatcgggtg cagcggatcg
      601 gcttctatgc catcggctcg ggtctggaca gcgatcgcga ggctttgacc gaggtgatca
      661 ggcagatccg gctctgaatg ctggcggact cggtcagaac accaatgcac tcgtcgtatc
      721 cacccgcggg tcggcggcgg cccgaagctc agcgccatcg acttcgatga tcgaaaccgg
      781 gccccaaccc ggctgccgct ccgggagctc ctcgatccgg tgcccgcggc gtcgcagctc
      841 ggccagggtc gacgacggca tcccgggctc gatggcggac ctcggcgccg agtagggacc
      901 atggtccgcg atcgtccatc gaggagctgc ctgcgccgtc tccaggtcct ggccggctat
      961 caccgctcgg gcggcgacct gggcgacaag ctggggctgg accgaaccgc ccctggtccc
     1021 gatgacccaa cgtgactggc cgtcgtcggt ccagagcgtt ggtgacagcg tgtgcaatgg
     1081 ccgcttgtgc ggcatcagct cgttggggtg gcccggtgtc agtgtgaagc caccaccacg
     1141 attgtggagg agaaatccgc ctctcggagc gccgaaggcg gacccggtcc cggagtaatt
     1201 ggaatggatg attgagaccg ccgttccctc ggcatcgacc acgcataggt aggcggtgct
     1261 cgattcggtg cccatgctcc gaggccaaat cccggtctgc tcccgatgta cggtcgccgc
     1321 tgccctccgc aaccgatcgt ggttcagcag cagatccgca ggcaggggtg cggtttcggg
     1381 gtcagccacg atgtcgtcgc gctcccaggc caccgcccgg aacgcttcga tgagaagatg
     1441 gagccagtcc gggtcttctg ggtcatctgg tgggtcgagc atctcgaaga cggcgagcgt
     1501 ggccggcccg agatacccct gagagttcgg ggggatggtc cacgcgctga ctccggtcac
     1561 gtcgcaggag atcggcggga cccaatcggc gtgggaccgg gcgaggtcgt cgagtgtgat
     1621 cgtcccgtcg agctcattga cgatctcaac tgccggttcg ccccgataga aggcgtcccg
     1681 cccatccctc gccacagctc gcaacgtgac cgccagggga attcgctgaa cgatgtcccc
     1741 ggcagagacc acgtcaccgt cgggatagaa ctcgtgaacc gcgggatgcg tggaatacat
     1801 cgcctcggtc tggcggaaag ccttcgcctg ctcggtcgag accgcgaagc ctccttctgc
     1861 gagctcgatc gccggctgca gacagtcggc gagtcccaac actccgagct ctgcggaaag
     1921 ggcctgccat ccgtcgacac agccggggac tgtcacggtc aaggggtgat cgcgtgggat
     1981 ctcggccccg ccgagcgaac gcaggacctc cgggtcggcg ttggacccag ctcgaccaga
     2041 cgcgttgaga gcgagcggac ggtgccatcc cgggcggtag atcagggcga agagatcccc
     2101 gccgatgccg caggtctccg gtgccacgac cccctgcgcc gccgtggcgg cgatagccgc
     2161 atccaccgca ttgcctccgg caagaagaac ccgtcgggca gcctcggctg agaggtagtg
     2221 gggggtggcg accgcgtgtg tcaggacagt ttgaggtcgg ggaggaaacg gttcagggcg
     2281 tcgagcacgg ctctcaccac ggccctgccc tcctcgtcct ccacgtaggc gcagccgatg
     2341 agcatccgct cgctggcctc ggtgaccacc accacttggg cgacggcgac ctttcgattc
     2401 ccgaggagtg gcacgtccat cgagttgatg gccagggccg cctctttgtg aatcccctgg
     2461 cgaaccgcat ccagcgtggc ctcggcgatc tggcgcgccc gggttgaagc cgcggcggcg
     2521 ccggtcgaag tgccggagag gatctcgttc tcccagcgaa gactcaccgt gacctcggct
     2581 cgagagcctt ccagggtctc ggccacgtcg atcagggcgg gacggaatcc accttccagg
     2641 tccccgtcgg ccaactgcac aacggagaca acacgccgat cgatctcctg gccgtagaga
     2701 gcttggctga gggattggac gtcgcgcacc aattgcttgg gcggcttatt tcgccgcgcc
     2761 aggacatgga tctcggagat gtgcccgttt ccgtagacga cgcgcgccgc ctcgacaccg
     2821 tcgatccgtc cgatcttctc ttgaaatgcg actatgtcca tgcccggtca gggtacatcc
     2881 gggtgtcaag ccctgatggg atagtcgctg gtccccttgt tcccggtaat caacaggtct
     2941 agattgctgg gcgctggagc tattcatggg aacgtggggc aaccgaggtc tttgacccgc
     3001 ttgcccggtc cgtggcgggc gaagtcaggg gcctcttttg acatgtgaag agttcccgtg
     3061 aaaacgggaa ctgggtgaga ccggcgtcag acgtcggcct gtaagcaatt ggacgccatc
     3121 tacccaagcg gagcggaacg tctgggcggc aacacaa
//
LOCUS       DBNHXU010000155         2731 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4811328, whole genome shotgun sequence.
ACCESSION   DBNHXU010000155 DBNHXU010000000
VERSION     DBNHXU010000155.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4811328"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1528)
                     /locus_tag="AC161X_03990"
     CDS             complement(140..1528)
                     /locus_tag="AC161X_03990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013454372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="di-heme oxidoreductase family protein"
                     /protein_id="HIR4988740.1"
                     /translation="MTSVTRPRKGRARIGVWSVAGLVLVATACSSATDDLMVEASGGE
                     GTTSASNQEAFGMSARNLTDAERRDFVVGNSFFTQNWVTAPSSTEDRDGLGPLLNGQG
                     CASCHVRDGRGTPEEGEPGLLFRLSVPGESGPVPEPVYGDQLQDRSILGVPAEGQVLR
                     LYVEEPGAFADGETYSLRRPMYEFDDLAYGPISVEVEVSPRLAPAVIGAGLLEAIPEA
                     DILGTADPDDADGDGISGRPNWIKDPTSGEPTLGRFGWKANVTSVERQVAGAFIGDIG
                     ITSHFFPDQNCTETQTACSAAPSGGEPEIPDDRMEKVVFYNQTLAVPARRNLDSPDVE
                     AGAGLFSDIGCVGCHQPRQETGDAEIAALANQVIFPFTDLLLHDMGPDLADDRADGEA
                     SGSEWRTPPMWGIGLTETVSGHTFFLHDGRARSLEEAILWHGGEAGPAASEFKALSSE
                     QRSQLLAFLGSL"
     gene            complement(1530..2648)
                     /locus_tag="AC161X_03995"
     CDS             complement(1530..2648)
                     /locus_tag="AC161X_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016447976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HIR4988741.1"
                     /translation="MSSALALSACGNDSSADLETEVVANYADVVHETYSMSLASATEM
                     GAEIDALAADPTDANLAAAKDAWLAARDDYGLTEAFRFYGGPIDAADTGPEGLINAWP
                     LDEAYIDYVEGAAESGVVNLPDEYPTIDADLLVSLNEAGGETNISTGWHAIEFLLWGQ
                     DLSTDGAGARPVTDFTTSPNADRRVAYLTVASDLLVGHLQSLVDAWAPDEDNYRAEFL
                     ALTTDEALGMILTGLGELSRGELAGERLTVAYEEHSQENEHSCFSDNTTSDIVANAAG
                     IQNVYLGAVGDVDGPGIHDLFEAADSDLASKLQDEIAASVAAAESIPAPFDKSIAPGV
                     PDDDPGRVAMFDTILALELQADTLVEAATAAGLVLNLS"
BASE COUNT          489 a          902 c          829 g          511 t
ORIGIN      
        1 caggcacaga gcaccagcgc catcacggcc gctccgaggc gtctcaccca cgactgaatt
       61 cttccctgac catcgttttg gcgcggcgat atcgacctat gggactcgat ctccgcgcca
      121 aaacggactc tggggtgcct cacaacgatc ccaggaaggc gagaagctga gatcgctgct
      181 ccgaagacag tgccttgaac tcggatgcag caggcccggc ctctccgccg tgccagagga
      241 tggcctcttc gagagatcgg gcccggccgt catgaaggaa gaaggtgtga ccgctgaccg
      301 tctccgtgag ccctatcccc cacatcgggg gggtccgcca ctcgctccct gacgcttcgc
      361 catcggctcg atcatcggca aggtctggcc ccatgtcatg caacaggagg tcggtgaacg
      421 gaaagatcac ctggttagcc agtgccgcga tctcggcgtc gccggtctcc tgtcttggct
      481 gatggcatcc gacgcacccg atgtcgctga acagccctgc gccggcctct acgtcgggag
      541 agtcgaggtt gcgtcgggcg ggaacggcga gagtctggtt gtagaacacc accttctcca
      601 ttcggtcatc cgggatttcg ggctcacccc cgctgggtgc ggcggaacat gcggtttggg
      661 tctcggtgca gttctgatcg gggaagaagt ggctggtgat cccgatgtca ccgatgaaag
      721 ccccagccac ctgacgctcc accgatgtca cgttcgcctt ccatccaaag cggccaagtg
      781 tcggctcgcc acttgtcgga tccttgatcc agttcgggcg tcccgagatc ccgtcgccgt
      841 cggcatcatc aggatccgcg gtaccaagga tgtcggcttc gggaatcgcc tccaagagcc
      901 cggccccgat aactgcaggt gccagcctcg gtgacacctc gacctccacc gagattgggc
      961 cgtaggcgag gtcgtcgaac tcgtacatcg gcctgcgcag gctgtacgtc tccccgtcgg
     1021 caaacgcgcc cggctcctcc acgtaaagac gcaacacctg cccttctgcc ggaacgccga
     1081 ggatcgagcg gtcctggagt tggtccccat acaccggctc cggaaccggg ccgctctcac
     1141 cgggcacgct cagtcggaaa agcaggcctg gttcaccctc ctcaggtgtc ccccgcccat
     1201 cccgaacatg gcacgaagca caaccctgcc cgttgagcaa gggacctaac ccgtctcgat
     1261 cctcggtcga ggagggcgcc gttacccagt tctgggtgaa gaagctgttc ccaaccacga
     1321 agtcccttcg ttcggcgtcg gtcagattgc gcgccgacat cccaaatgcc tcctggttgg
     1381 aagctgaggt agttccctcg cccccggacg cctcgaccat gaggtcgtcg gtcgcggatg
     1441 agcaagcagt cgccaccaaa accaggccag caacggacca cacgccgatt cgtgcccgtc
     1501 ccttgcgtgg cctggtcacc gatgtcacat caactcaggt tcagcacgag acctgctgcg
     1561 gttgccgctt ccaccaacgt gtccgcctga agctcgaggg cgagaatggt gtcgaacatg
     1621 gcaacccggc ccgggtcgtc atcgggaacg cccggagcaa tgctcttgtc gaatggggcc
     1681 gggatcgact cggccgcggc gacactcgcc gcgatctcat cctggagctt tgacgcgagg
     1741 tcggaatcgg cagcttcgaa gagatcgtgg atccccgggc catcgacgtc tccgactgca
     1801 ccgagataca cgttctgtat accggcggcg ttggcgacga tgtccgacgt cgtgttgtcg
     1861 gagaagcaag agtgctcgtt ctcctgcgag tgctcctcgt aggcgacggt gagacgctcc
     1921 cctgcgagct cgccgcggct caactcgccg agaccggtga ggatcatccc cagcgcctca
     1981 tcagtggtca acgcaaggaa ctcggcgcgg tagttgtctt cgtcaggtgc ccaggcatcg
     2041 accagagact gcaaatgacc gacgagcagg tccgaggcca cggtgaggta tgcgactcga
     2101 cggtccgcat tgggagaagt cgtgaagtcg gtgacgggcc tcgctcccgc tccgtcggtg
     2161 gataggtcct gtccccagag caggaattcg atggcatgcc atcccgtgga gatattggtc
     2221 tctcccccgg cctcgttgag tgacaccagg aggtcggcgt cgatggtcgg gtactcatcg
     2281 gggaggttga ccactccgga ctccgctgcg ccttcgacat agtcgatgta tgcctcgtcc
     2341 aggggccagg cgttgatcag gccctccggc ccggtgtctg cggcgtcgat ggggcctccg
     2401 taaaagcgga aggcctcggt gagcccgtag tcgtcacggg ccgccagcca agcatccttc
     2461 gccgcggcca gattggcgtc ggtcggatcg gcagccagag cgtcgatttc tgcgcccatc
     2521 tcggtggccg aagccaggct catcgagtac gtctcgtgca ccacgtccgc gtagttggcc
     2581 accacctcgg tctccagatc agcgctgctg tcgttcccgc aggccgatag tgccagcgcc
     2641 gagctcatca gggcgacgac gcttctcctc catctcattt gatctcctag ctcccaaact
     2701 cgttcggtag ctaaagatat ttgcactatg g
//
LOCUS       DBNHXU010000156         6276 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2430413, whole genome shotgun sequence.
ACCESSION   DBNHXU010000156 DBNHXU010000000
VERSION     DBNHXU010000156.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2430413"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            23..1351
                     /locus_tag="AC161X_04000"
     CDS             23..1351
                     /locus_tag="AC161X_04000"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019960029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HIR4988742.1"
                     /translation="MATNRDIRQTIVHLLSNMSDGKEIRSYLQRFSELDRSRFAVIKI
                     GGAILRDSLEETAAALAFLHTVGLTPIVLHGGGPQLDETLAKRGITSEKIDGLRVTGP
                     EVLDAARDTFIEQNILLVEAIRDQGVAAHGLTEGAFDADYVDRERYGLVGEPTEVHLP
                     LIQSIIASDAIPILTCLGVAPGGQLVNMNADSATRALVHAVQPMKIIFLAAVGGLLDA
                     DDRLIESINLASDYEHLMSQEWVHSGMRLKLAEIKLLLDDTPLSTSVSITTPFGLVKE
                     LFTHGGSGTMVRRGELIEKVTDKLALDGGQVEKLVEEAFGRPLDPNWWTELELEAAYI
                     SESWRAGALIARIGDFAYLDKFAILEDARGEGLARTVWAQVVTDYPVLYWRSRIENGF
                     NAFYADEADGSVKRGPWTIFWKGEPDFVNIAARVEAIASLPASFIGHHDE"
     gene            1344..2267
                     /gene="argC"
                     /locus_tag="AC161X_04005"
     CDS             1344..2267
                     /gene="argC"
                     /locus_tag="AC161X_04005"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019660497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_function: GO:0070401 - NADP+ binding [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HIR4988743.1"
                     /translation="MSDRFTVGLVGARGHTGAEIVRLMRDHPSLELVYAGSRELAGQS
                     VPGLDGMIYESIEPTDVSNLETDVVILALPDGAAASFLEAVTADEIIVDISFDHRLDD
                     AWAYGLPELRRDRLKGATRIANPGCYATAMELVLAPLTDMISGVPAVFGVSGYSGAGS
                     TPGPRNDLERLEDNLMPYKLVGHNHELEASHQLGMPIRFMPHVHQAFRGLIVTANVPL
                     SDETSSEEIRSRFENAYGAEPLIELQEGIPELKDGTNRLGVLIGGFNTSEDGHHAVVV
                     AALDNLLKGAAVQALQNVNLALGLPELAGIV"
     gene            2337..4697
                     /locus_tag="AC161X_04010"
     CDS             2337..4697
                     /locus_tag="AC161X_04010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HIR4988744.1"
                     /translation="MAARARLTYFVSTESQPRARRPADAFTVFAGLVLLLLGIFGIDR
                     HPPWETALTDFLKSLPSWVESMFALGYAFGLLYGLGILIAVITGGRERRGALRDVSIA
                     AVVAPILGAALGFALTGFWPYVLPEIGLDSPDPRFPVLRVVIITAILVTASPHLSRPL
                     RRLGWLTILTIGASAIALEFGMFSDAVGSLGMGLVVAGTVLLIFGSPRGYPDPTSVAI
                     AMKALGLPVFNVEIDPEQSWGVRRLKASTENGTPITIKAYGRDATESQFAAKVWRTLW
                     YRENGQMIGYSRIQAAEHEALVTLLAQRAGARVPEMLAVGEGSSEIALLAVTAGGTPL
                     PELDPADISDDTLIGIWEDLTRLHSSSIAHGSLNASAIQVGADGHMIADFALGSLSAG
                     EVERAHDVVELLFSLSLAVGAERAVETASNGLGTDTLSSVLPYLQLPAISPSTKDQAE
                     KPKDVMKQLQAAMVAVTGQDLPELAKLRRVSGRNILMTSLLLLVAWALIPLLTSVDYA
                     AVWAVLETANWALIVIALIVGQSMFYPQATATMFAVPRPLPFWPLVVLNLAAKFVSLA
                     IPGAAGRVAMNTAFLRKFGVSITVAVTQGAVDTFSGFLVQCVILIIGFVSSDVDLDID
                     TVDVNWALILLVVVLVAVGVVVAVLRIRKLRDRILPVVQQGWGTLVSVLRQPSRAFGL
                     LGSNLVYWLILGTTLWLVLEAIDVQISFGAALVVAVATDLLGGFVPIPGGVGVAEAVM
                     TGFLTALGVDQSAAFAGTITYRFITFYLPAFEGFFAMHWLERNEYI"
     gene            4720..6021
                     /locus_tag="AC161X_04015"
     CDS             4720..6021
                     /locus_tag="AC161X_04015"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HIR4988745.1"
                     /translation="MTFTAVELIATKRDRGALSPEAIEWLIRAYTEGSVTDYQMSAMA
                     MAIFFNGLDPDELAAWTAAMLHSGQILDCSDIPKPKVDKHSTGGVGDKISIPLAPLVA
                     ACGVAVPMMSGRGLGHTGGTLDKLETIPGFTTGLDPKRFHEVLREHGLVLAGQSETLV
                     PADRKLYALRDATATVPSIPLISSSIMSKKLAEGLDGLLLDVKTGSGAFMQDLAKSRE
                     LARTMVGIGIRNQVKTVALITRMDQPLGREVGNANEMRESIDVLNGGGPPDVVELTLA
                     FGEVMLELAGESGGRDRLVEAIANGAARQKLIDVVAAQGGDTSVIDDPSLLPTAPNEA
                     TVNADRDGYVTRCDALVVGSVATRLGAGRERKEDIVDPGVGITVLAKLGESISKGQPL
                     ARVRFTDPARWDAQQEILAGAWAVGDQPPEMEALVVERVDG"
     gene            6072..>6276
                     /locus_tag="AC161X_04020"
     CDS             6072..>6276
                     /locus_tag="AC161X_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR4988746.1"
                     /translation="MKVVVCVKQIPDPATPYQLESGTNWLLRPDDQILDDTDRYGVEM
                     GLQLAQASEGTVTLVCMGPAGNGQ"
BASE COUNT         1126 a         2022 c         1938 g         1190 t
ORIGIN      
        1 acaagaattg acacgacttg caatggcgac caaccgagac atccgacaga ccatcgtcca
       61 cttgctgagc aacatgagcg acggcaagga gatccgctcg tacctgcagc gtttctcgga
      121 gctagacagg tcgcgttttg cggtgatcaa gatcggcggc gccatcctcc gcgactcact
      181 ggaggagact gcagcagccc tcgccttcct ccacactgtc ggcctcaccc caatcgtcct
      241 ccatggaggt ggcccccaac tcgacgagac cctggcgaag cgcggcatca ccagcgagaa
      301 gatcgacggt cttcgggtca ccggccctga agtcctcgac gcagcccggg acaccttcat
      361 cgagcagaac atccttctcg tcgaggcgat cagagatcag ggcgtggccg cccacgggct
      421 caccgaaggc gccttcgacg ccgattatgt cgaccgcgag cgatacgggc tcgtcggaga
      481 gcccaccgag gtccacctcc cactcattca gtcgatcatc gcctcagacg cgataccgat
      541 cctcacctgt ctcggggtgg cccccggagg ccagctggtc aacatgaacg ccgactccgc
      601 cacgagggcg ctggtccacg ccgtccaacc gatgaagatc atcttcttgg cggccgtcgg
      661 cgggctgctg gatgccgacg accggctgat cgagtcgatc aatctcgcct ccgactatga
      721 gcacctcatg agccaggaat gggtgcattc cggaatgcgc ctcaagctgg ccgagatcaa
      781 gcttctcctg gacgacaccc cgctctccac atctgtgtcg atcacgacgc cgttcggcct
      841 ggtcaaagag ctgttcaccc atggcgggtc gggaacgatg gtcaggcgtg gtgagctgat
      901 cgagaaagtg accgacaagc tggcattgga cggcggccag gtcgagaagc tggtggagga
      961 ggctttcggg cgcccccttg accccaactg gtggaccgaa ctcgaactcg aagcggcata
     1021 tatctcggag agttggcggg ccggtgctct gatcgcccgg atcggggact tcgcatacct
     1081 cgacaagttc gcgatcttgg aagatgcccg aggcgaagga ttggcacgga cggtttgggc
     1141 tcaggtcgtc accgactacc ccgtcctgta ctggcgctcc cgcatcgaga atgggttcaa
     1201 cgccttctat gccgatgagg cagatgggtc ggtgaagcgg ggaccgtgga ccatcttctg
     1261 gaagggcgag cccgacttcg tgaacatcgc cgcccgtgtc gaagcaatcg cctcgcttcc
     1321 cgcctccttc atcggccacc acgatgagtg atcgattcac agtcggcctg gtcggcgccc
     1381 ggggccacac cggagcagag atcgtcagac ttatgcggga ccacccgtcc ctggaactgg
     1441 tctatgccgg ctctcgcgaa ttggctggcc agtccgttcc cgggctcgat ggaatgatct
     1501 acgagtcgat cgaaccgacc gacgtgtcca acctcgagac ggatgtggtg attctcgctc
     1561 tccccgacgg cgccgctgcc tcgttcctcg aagctgtaac cgccgacgag atcatcgttg
     1621 acatctcgtt cgatcaccgt ctcgacgacg cctgggccta cgggctgccc gagttgcgcc
     1681 gggaccggct taaaggtgcg acccggatcg caaacccggg ctgttacgcg accgccatgg
     1741 aactggtgct ggcacctctc accgacatga tctccggtgt tcccgccgtg ttcggtgtgt
     1801 ccggttactc cggagccggg tctacccccg gcccgcgcaa cgacctcgag cgactcgagg
     1861 acaacctgat gccttacaag ctcgtgggcc acaaccacga gttggaggca tctcatcaac
     1921 tggggatgcc gattcggttc atgcctcacg tccatcaggc ttttcgaggt ctgatcgtga
     1981 ccgccaacgt gccgctgagc gacgaaacga gcagcgagga gatccgctcg aggttcgaga
     2041 acgcctacgg agcggagccc ttgatcgaac tccaggaggg gatccccgag ctcaaagatg
     2101 gcacgaaccg gctcggagtc ttgatcggtg gcttcaacac cagcgaagac ggtcaccacg
     2161 cggtggtggt cgcggcactg gacaaccttt tgaaaggggc tgccgtccag gccctgcaga
     2221 acgtgaacct tgctctcgga ctccccgagc tcgccggaat cgtgtaggaa tccgacttcg
     2281 acagagggag tcgcgtgggc ctacctccag aacctgagag tgccgagccg atctcgatgg
     2341 ctgctcgcgc ccgtctcacc tacttcgtct cgacggagtc ccagccgagg gctcgacgcc
     2401 ctgctgatgc cttcaccgtt ttcgccggac tggtccttct cctgctgggc atcttcggga
     2461 tcgaccgcca cccaccttgg gaaacggccc tcaccgactt tctgaaatcc ctcccctctt
     2521 gggtggagtc gatgttcgcc cttggttacg ccttcggcct tctgtatggg ctcggaattc
     2581 tgatcgcggt gatcaccggg ggccgggagc gcaggggggc tctccgtgac gtctcgatcg
     2641 ctgcggtggt tgccccgatc cttggcgcgg ccctcggttt cgccttgacc ggattctggc
     2701 cgtacgtgtt acccgagatc gggctcgact cccctgatcc gcggttcccg gtgcttcgag
     2761 tagtgatcat caccgcgatc ctggtgactg catcaccaca tctgagccgt ccgctccgcc
     2821 gtctggggtg gctgaccatc ctgacgatcg gcgcctcggc aatagccctc gaattcggca
     2881 tgttctcgga tgccgtgggc tcgcttggga tggggctagt cgtagccggg actgttctcc
     2941 tgattttcgg ctccccgcgt ggctatccgg acccgacctc ggtcgccatc gcgatgaagg
     3001 cccttggcct tcccgtgttc aacgtcgaga tcgacccgga gcagtcgtgg ggcgttcgca
     3061 ggctcaaggc cagcactgag aacggcacac cgatcacgat caaggcctat ggaagggacg
     3121 ccaccgaatc acagttcgca gccaaggtct ggcggaccct ttggtaccgg gagaacgggc
     3181 agatgatcgg ctactcccgc atccaggccg cggaacatga agcactcgtc actcttctgg
     3241 cccagcgggc aggagcccgg gtcccggaaa tgctcgcggt cggtgaggga tcgtccgaga
     3301 ttgcgctact ggccgtcacg gcaggcggga cccctcttcc cgaactcgat ccggcggaca
     3361 tcagcgacga caccctcatc gggatctggg aggacctgac caggctccat tcctcttcga
     3421 ttgcacatgg gtcgctcaac gcgagcgcga ttcaggtcgg agccgacgga cacatgatcg
     3481 ccgacttcgc gctgggttcg ctctccgcag gtgaggtgga acgagcccat gacgtggtcg
     3541 aactgctctt ctccttgagc ctggctgttg gcgccgagcg agctgtcgag actgcttcga
     3601 acgggctcgg cacagacacc ctctcctccg ttctccccta cctgcaactg ccggcgatca
     3661 gccccagcac caaggaccag gccgagaagc ccaaggacgt gatgaagcaa ctccaagcag
     3721 ccatggtcgc tgtcaccggc caggacctgc cggaactggc gaagctgcgg agggtcagcg
     3781 ggcgcaacat cctgatgacc tcgttgctcc tgctcgtggc ctgggcattg attcccctgt
     3841 tgaccagcgt cgactatgcc gcggtatggg cggtgctgga gaccgccaac tgggccttga
     3901 tcgtcatcgc cttgatcgtc ggtcagtcga tgttctaccc ccaagcgacc gccacgatgt
     3961 tcgctgtgcc acggccgctt ccgttttggc cgctcgtcgt cctcaacctg gccgccaaat
     4021 tcgtgagcct cgcgataccg ggcgccgccg ggcgggtggc gatgaacact gcgttcctcc
     4081 gcaagttcgg agtctcgatc acggttgcgg tgacccaggg tgccgtcgac accttttccg
     4141 gattcctggt ccagtgtgtg atcctgatca tcggctttgt ctccagtgac gtagacctgg
     4201 acatcgatac cgtcgacgtc aactgggcac tcatcctcct ggtggttgtc ctggtcgcgg
     4261 tcggcgtggt ggtcgctgtg ctgcggatca ggaagttgag ggaccgcata ctccccgtcg
     4321 tccaacaggg ttgggggacc ctggtctccg tgcttcgcca gccgagccgg gcgtttgggc
     4381 tcctgggcag caatctcgtc tactggctga tactggggac gacgctttgg ctcgtcctcg
     4441 aggcgatcga tgtccagatc agctttgggg cagcgcttgt cgtcgccgtc gccaccgatc
     4501 tcctcggagg cttcgtcccg atcccgggcg gagttggggt cgccgaagca gtcatgaccg
     4561 ggttcctcac cgcgctcggg gttgatcaat ccgcggcatt cgctgggacc atcacctacc
     4621 gattcatcac cttctatctg cccgcattcg aaggattctt cgccatgcat tggttagagc
     4681 gcaacgagta catctgaggc cgatagcgtc cctcgtctaa tgacgttcac cgcagtcgaa
     4741 ctgatcgcga ccaagcgcga tcggggtgcc ctgagccccg aagcgatcga gtggctgatt
     4801 cgggcctaca ccgaaggatc ggtcaccgac taccagatgt cggcgatggc gatggccatc
     4861 ttcttcaacg ggctcgaccc cgacgagttg gcggcatgga ccgcggccat gctgcactcg
     4921 ggccagatcc tcgattgctc ggacatcccc aaaccgaagg tcgacaagca ctcaacaggc
     4981 ggggtcggcg acaagatctc gatcccattg gctcctctgg tcgcggcgtg cggcgtggcg
     5041 gtgccgatga tgtcggggcg cgggctgggc cacaccgggg gcacgctcga caaattggag
     5101 acgattcccg gattcaccac gggtctcgac ccaaagcggt ttcacgaggt gctcagggag
     5161 cacggactgg ttctggccgg ccaatccgag accctggtcc ctgccgatcg caagctctac
     5221 gcactccgtg acgccaccgc aaccgtgcct tcgattccgc tcatctcctc ttcgatcatg
     5281 tccaagaagc tggctgaggg actcgacggg ctcctcctcg acgtgaaaac cggttccgga
     5341 gcgttcatgc aagacctggc gaagtcgagg gaattggccc gcaccatggt tgggatcgga
     5401 atcaggaacc aggtcaagac tgtggcattg atcacccgga tggaccagcc ccttggccgg
     5461 gaggtcggga atgccaacga gatgcgagag tcgatcgatg tgctcaacgg cggaggccct
     5521 cccgacgtcg tcgagctgac cctggccttc ggcgaggtca tgctcgaact agccggcgaa
     5581 agcgggggtc gagaccgtct ggttgaagca atcgcgaacg gggctgctcg ccagaaactg
     5641 atcgatgtgg tcgccgccca aggaggggac acttcggtga tcgacgaccc ctcactcctc
     5701 ccgaccgccc ccaacgaagc gacagtcaat gccgaccggg acgggtatgt cacccgctgt
     5761 gatgccctgg tggtcggatc ggtggcgacc cgtcttggag cgggacggga gcgcaaggag
     5821 gacatcgtcg atcccggtgt cgggatcacc gtgctcgcca aactcggaga gtcgatatcc
     5881 aagggtcaac ccctggcccg ggttcgcttc accgaccccg cccgatggga tgcccagcag
     5941 gaaatacttg ccggagcctg ggctgttggg gaccaaccac ccgagatgga ggccctggtc
     6001 gtggagcggg tcgacgggta gtgggctctc gttaccatcc ccggacccaa tccacctagg
     6061 agttgaaccg aatgaaggtc gtcgtctgcg taaagcagat ccctgacccc gccaccccgt
     6121 atcagctcga gagcggcacc aactggctgc tccggcccga cgaccagatc cttgacgaca
     6181 ccgatcgcta cggggtcgag atgggactcc aattggctca ggccagtgaa gggacggtga
     6241 ccctggtgtg catgggcccg gcggggaacg ggcaag
//
LOCUS       DBNHXU010000157         2592 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4613328, whole genome shotgun sequence.
ACCESSION   DBNHXU010000157 DBNHXU010000000
VERSION     DBNHXU010000157.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2592)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2592)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2592
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4613328"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..554
                     /locus_tag="AC161X_04025"
     CDS             <1..554
                     /locus_tag="AC161X_04025"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR4988747.1"
                     /translation="DNGHDLVVLMGSPWSVYEDRLQGWVAPELDFIRRQASSGVPILG
                     ICFGAQAMSAAMGGKVTRATRPEYGWVTIDSEVDEIAGGPWFQYHHDEFTLPPGATAL
                     AQNASGLQAFRSGKNLAVQFHPEMTSSLIASWCEVDGGDEMVDVGIDPDQVIEESRLL
                     EKTTQPSLERMLDWFLEDIAGAM"
     gene            551..1807
                     /locus_tag="AC161X_04030"
     CDS             551..1807
                     /locus_tag="AC161X_04030"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HIR4988748.1"
                     /translation="MTRERPMVRVDLTDPDLWLGGVPHDEFRRLRSESPIAWSQHPSG
                     HRGFWVVAGHEDLVAISRDPATFSSRDGVITLDDLEGDQLEARRTMLEEDSPRHRDLR
                     GLTSAHFTPKAVRAHEAYVRALAVKAIDAAVLAGECDLVSELSELVPIRVLGKILGVP
                     DENVDDLVRLGNQLIGSDDPEYLDPMLGEADPVETRLLPFGHPASFEAFQMAWEIAAT
                     RRSDPAEDVMTALALGEIDGEPLTDQELGTYFVLLVIAGNETTRHSITSGIAALAANL
                     EQWEQLRSGALDPNRAADEVLRWATAIHFHRRVATTDTLIGEVEIEAGEKVALYFASA
                     NFDEAVFEDPYQLNLDRSPNDHVAFGRGGPHFCLGAHLARLEVRVVLEELAARVSSIE
                     VIGEPVRLRSNHINGIKHLPVRLRPS"
     gene            1851..2258
                     /locus_tag="AC161X_04035"
     CDS             1851..2258
                     /locus_tag="AC161X_04035"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007355538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988749.1"
                     /translation="MITYRLGNDLDLDAVIDLYVNSTIGERRPVGERERMKTMMEEAD
                     LVVTAWDGDLMVGISRSVTDWVYCTYLSDLAVRLDYQHQGIGKELVRRTRQETPQATV
                     ILLAAPKAVDYYPKIGMTQHNSAWILPAGVEPL"
     gene            complement(2267..>2592)
                     /locus_tag="AC161X_04040"
     CDS             complement(2267..>2592)
                     /locus_tag="AC161X_04040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR4988750.1"
                     /translation="VGLAGPYDVARLGLLMYPFFGAGPADDPEAWREGNPLNLVDLNP
                     DLESLLMYGEEDGFVEPSFAFDFGDALTGAGSEALVEGVEGAQHNDMYLPRIVGDLIA
                     TWLER"
BASE COUNT          486 a          816 c          817 g          473 t
ORIGIN      
        1 cggacaacgg gcacgacctg gtggtcctga tgggatcacc ttggtcggtt tacgaggacc
       61 ggctgcaggg ctgggttgcc cccgagctcg atttcatccg acgccaggcc agttcaggcg
      121 tccccattct cggcatctgc ttcggcgcac aagccatgtc cgccgcgatg ggtggcaagg
      181 tcacccgggc gacgcgcccg gaatatggct gggtgaccat cgactctgaa gtcgacgaga
      241 tcgccggcgg gccatggttt cagtatcacc atgacgagtt cacccttcca cctggggcga
      301 ccgcgctggc ccaaaacgcc tcgggcctgc aggctttccg atcggggaag aacctcgccg
      361 tccagttcca ccccgagatg acatcgagtc tgatcgcctc ctggtgtgag gtagacggtg
      421 gtgatgagat ggtcgatgtc ggcatcgacc ccgaccaggt gatcgaggag agccggcttc
      481 tggagaagac cacccaacct tcgctggagc ggatgttgga ctggttcctc gaagacatcg
      541 ctggagccat gtgacccgag agcggccgat ggtcagggtc gacctcaccg atcccgacct
      601 ctggctgggc ggggtgcccc acgacgagtt tcgaaggctg cgctccgagt cgccgatcgc
      661 ttggtcccag catccgtcgg gtcatcgcgg attctgggtg gtcgccggcc atgaggatct
      721 ggtcgcgatc tcacgggacc cagcgacctt ctcctcacgg gacggagtca tcaccctcga
      781 cgatctcgaa ggcgaccagc tcgaagcaag acggacgatg ttggaggaag actctccgcg
      841 tcaccgcgac ttgagaggcc tgacgtcggc ccacttcacg ccgaaggcgg tgcgagccca
      901 cgaggcatac gtccgcgctc ttgcggtgaa ggcgatcgat gccgccgtct tggccgggga
      961 gtgcgaccta gtttccgagt tgagcgagct cgtcccgatt cgggttctgg gaaagattct
     1021 tggtgtccct gacgaaaacg tcgacgacct ggttcgtctc ggcaaccaac tgattggctc
     1081 cgacgacccc gagtacctcg atccgatgtt gggggaagcg gacccggtgg agaccagatt
     1141 gcttcccttc ggccaccccg cgtcattcga ggcgttccag atggcgtggg agatcgcggc
     1201 aacacggcgt tccgatccag cggaagacgt gatgaccgcg ctggctcttg gtgagatcga
     1261 cggcgagcct ctcaccgatc aggagctggg cacctatttc gtcttgcttg tgattgccgg
     1321 caacgagacc acccgtcaca gcatcacctc cggaatcgcg gccctggcgg cgaatctcga
     1381 gcaatgggaa cagttgcgct ccggggccct cgaccccaat cgggccgccg acgaggtgct
     1441 gcgctgggcg actgcgatcc acttccatcg gcgcgttgcc accaccgaca ccttgatcgg
     1501 cgaggtcgag atcgaagccg gtgagaaggt cgctctgtat ttcgcgtcgg cgaacttcga
     1561 cgaggctgtc ttcgaggatc cctatcagct gaacctggac cgatctccga acgaccacgt
     1621 ggctttcggg cggggcgggc ctcatttctg tctcggcgcc cacctggcgc gactggaagt
     1681 cagggtcgtg ctcgaggagc tggcagccag ggtctcatcg atcgaggtga tcggagagcc
     1741 agtcaggctc cgctccaacc acatcaacgg gatcaagcac cttccggttc ggcttcggcc
     1801 aagctgacaa cagtcggtca ccggggaaag gacgctctac tctccgacgt gtgatcacct
     1861 atcgattggg caatgacctc gacctcgatg ccgtcatcga cctatacgtc aactccacca
     1921 tcggtgagcg ccgcccggtg ggtgagcggg aacggatgaa aaccatgatg gaagaggcag
     1981 atctggtggt cactgcctgg gacggcgatc tcatggttgg gatatcacgc tcggtgaccg
     2041 actgggtgta ctgcacttat ctctcggatc tcgcggtgag actcgattac cagcatcagg
     2101 gcatcggcaa ggagttggtg cggaggaccc gccaagagac gccacaggcc accgtcatcc
     2161 tcctggccgc tccgaaggca gtggattatt accccaagat cggcatgacc cagcacaaca
     2221 gtgcctggat ccttcctgcc ggagtcgagc cgctctaacc gacgcctcag cgctccagcc
     2281 aggtcgcgat caggtccccg acaattcgag gcaggtacat gtcgttgtgc tgggcgcctt
     2341 cgaccccttc caccagggcc tccgaaccgg caccggtgag ggcatcgccg aaatcgaacg
     2401 caaaggaagg ctcgacgaac ccgtcctcct cgccgtacat gagaagactc tcgagatccg
     2461 ggttcaggtc gaccaggttg agcggattgc cttcacgcca agcctcgggg tcgtcggcag
     2521 gcccggctcc gaagaacggg tacatcagga ggcccaagcg ggcgacgtca tatggccccg
     2581 ccagcccgac ga
//
LOCUS       DBNHXU010000158         3653 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_49971, whole genome shotgun sequence.
ACCESSION   DBNHXU010000158 DBNHXU010000000
VERSION     DBNHXU010000158.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3653)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3653
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_49971"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(170..505)
                     /locus_tag="AC161X_04045"
     CDS             complement(170..505)
                     /locus_tag="AC161X_04045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR4988751.1"
                     /translation="MERAWRRWGVLSVATVVIVLSPVARAESGGFRNYVPIAIAVGES
                     DSVVVAKGDHLWSISAEHLEHRLARHPQDTEIGPYWHQVIEANRHRLRSGDPDLIYPG
                     ETVLLPEAG"
     gene            551..808
                     /locus_tag="AC161X_04050"
     CDS             551..808
                     /locus_tag="AC161X_04050"
                     /inference="COORDINATES: protein motif:HMM:NF022749.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3107 family protein"
                     /protein_id="HIR4988752.1"
                     /translation="MSGPTRQGAVMKVRIGVAQTNKVVEIEVDDVSSFKKELERAVAA
                     GELAWFADSKGRTVGVPASQVAYVEIEDAESGPAVGFAPAV"
     gene            complement(819..1091)
                     /locus_tag="AC161X_04055"
     CDS             complement(819..1091)
                     /locus_tag="AC161X_04055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR4988753.1"
                     /translation="MLTTITTDWRDLAACRDSEPTLFFPIGTTGPAVDQIDAAIAICS
                     TCTVQEDCLQYALETNQESGVWGGYAEDDRRRLRKRWLAERRRRAV"
     gene            1212..1910
                     /locus_tag="AC161X_04060"
     CDS             1212..1910
                     /locus_tag="AC161X_04060"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HIR4988754.1"
                     /translation="MSNTPLIIGHRGWPSRFPDNTLAGALAAASVADAIEVDVRRCAD
                     GKLVLSHNPDQEGRLVSTHGWDVLCELDLGGGHHPALLDEVIASLPETPLMLEVKNLP
                     HQPGFEPDHRVGLETASRARADDWVTSFHWPTVDAVHREFPGVATGLIVDGPEDLERA
                     VEHCLDVGHIAVVPNWKLLSTEAGRLLVTESGLQVHVWTVNDTGVAAELAAMGVTGII
                     SDDPGTIASIRDTG"
     gene            1907..2371
                     /locus_tag="AC161X_04065"
     CDS             1907..2371
                     /locus_tag="AC161X_04065"
                     /inference="COORDINATES: protein motif:HMM:NF020977.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HIR4988755.1"
                     /translation="MTIKDELAEELRDAMRAQDKPRRDVIRQVETEVAVARSQPGFEG
                     EADDELYRNVIGSYVKKMDKARDEYLEIGERGAAMAEKLGFEVQYLSRWLPKKLDEAA
                     TRELVRVAIAELGVAGDLKATGRVIGQVMKADGENLDGGLVSRVVREELAPD"
     gene            2368..3312
                     /locus_tag="AC161X_04070"
     CDS             2368..3312
                     /locus_tag="AC161X_04070"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF013252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR4988756.1"
                     /translation="MSVPDFEPMLATRWPAAFDDPGWWFEVKWDGYRAIVSAEGGRVK
                     ARSRRGLDLIGPFPELAGLPIPDGVALDGEVVAFDEDGRPSFSNLQQRTGFGGKGTRA
                     DVGVNLVVFDVLFAGEPIIGEPYEERRAQLERLALPAPIVVPEPLPEHGLSLFAAAEG
                     QGLEGIVAKRAGSPYLPGRRSPDWRKVAIRRRLRAVVGGYVPGERSRASTFGSILVGL
                     NDDQGLRWIAAVGSGFDDQALDAFSRALRELERPTKPFYNEVLVPNPRPPVWVEPGIV
                     VDVEFKEWTHDLHLRAPVYKGIEIADPDSVTWAEEGPG"
     gene            complement(3309..>3653)
                     /locus_tag="AC161X_04075"
     CDS             complement(3309..>3653)
                     /locus_tag="AC161X_04075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="non-homologous end joining protein Ku"
                     /protein_id="HIR4988757.1"
                     /translation="LRAKDGTIVMETMYWPDELREADFEELSADVEIRPEEVAMAASL
                     IDNLTRPFDAAAYVDPTREAVEDLATKKVAGEEIVAVASPEPTKVVDLLEALKASVEA
                     TKSKEVKTAKAG"
BASE COUNT          666 a         1080 c         1218 g          689 t
ORIGIN      
        1 agatgtggat caagtcgttc aagtacgtgg atggcgaggt caccgaggag cacgactgga
       61 ccgaagagct gggcactctc ggacaggtcg acagcttcgg gatcgggggc gatggcgagc
      121 tctacctggt cacctacgaa ggggcagtcg ccaagttcac cgcggttcgc taacccgcct
      181 cgggcaggag cacagtctcg ccggggtaga tgagatcagg atcacccgag cgaagtcggt
      241 ggcgattcgc ctcgatcacc tggtgccaat aggggccgat ttcggtgtcc tgaggatgac
      301 gggccaggcg atgctcgaga tgctccgcgg agatgctcca gaggtgatct cccttcgcga
      361 ccaccaccga atccgattcg ccaaccgcga tggctatcgg gacgtagttt cggaaaccgc
      421 cggattcagc cctcgccacc gggctgagga cgatcacaac tgtggccact gacaacaccc
      481 cccaccgacg ccaggcacgc tccatgactc gaacctaagg ccgagaacgg ggctccggaa
      541 gagggttacg atgagtggac cgacacgtca gggagctgtg atgaaggtcc gtattggggt
      601 tgcccaaacc aacaaggtgg tcgagatcga ggtcgacgac gtttcgtcct tcaagaagga
      661 gctggaacgc gcggtcgcag ccggcgagct tgcctggttc gcggacagca aggggagaac
      721 cgttggagta cccgcgagtc aggtggccta cgtggagatc gaagacgccg agagcgggcc
      781 tgcggttgga ttcgcccctg cggtgtgaat cgctccggtc agacggcgcg gcggcggcgc
      841 tcggccagcc agcgcttccg cagccgacgc cgatcgtcct cggcgtatcc accccagacg
      901 cccgattcct ggttggtctc gagtgcgtac tggaggcagt cctcctggac ggtgcaagtt
      961 gagcaaatgg cgatcgcagc gtcgatctga tctaccgccg gaccggtggt gccgatgggg
     1021 aagaacaggg tcggctccga atcccggcaa gctgccaggt cccgccaatc agtggtgatg
     1081 gttgtgagca cgaagttttc cttaatttgt gatctttttc acaagggtgc cggccgtttt
     1141 agaggccgca cgagggattt ccggtccgga atgtactggc cgaaccttca tttgtaaacg
     1201 agtttctcga gatgagtaac acgccattga tcatcggtca ccggggatgg ccctccagat
     1261 tcccggataa caccctggct ggagccctgg ccgcggcttc tgtagccgat gccatcgagg
     1321 tcgacgttcg gcgatgtgcc gacggcaagc tcgttctctc ccataacccc gatcaggaag
     1381 gtcgcttggt ttcgacccat ggctgggacg tcctatgcga gctcgatctg gggggtggcc
     1441 atcaccccgc cctcctcgac gaggtcatcg cctcgttgcc ggagacacct ctcatgctcg
     1501 aggtgaagaa tctcccccat caaccgggat ttgagcccga ccacagggtt ggtctcgaga
     1561 ccgcgtcgag ggctcgcgcc gacgactggg tgacttcgtt ccactggcca accgttgatg
     1621 ccgtccatcg cgaattcccg ggtgttgcca ccgggctgat cgtcgacggt ccggaagatc
     1681 tggagcgagc cgtggaacac tgtttagacg tcggccacat cgccgtggtt cccaattgga
     1741 agctgctcag caccgaggcg ggacgcctcc tcgtcacaga atccggcctc caggttcatg
     1801 tgtggaccgt caacgacacc ggcgttgcag cggagttggc cgcgatgggt gtcaccggca
     1861 tcattagcga tgacccgggc accatcgcct cgatcaggga cacaggatga ccatcaagga
     1921 tgaactggct gaagagctga gggacgcgat gcgagcccag gacaagccgc gccgcgacgt
     1981 cattcgccaa gtggagaccg aagtcgccgt tgcccgtagt cagcctggat tcgagggaga
     2041 ggcggacgac gagttgtacc gcaacgtcat cggctcctac gtcaagaaga tggacaaggc
     2101 ccgtgacgag tacctcgaga tcggtgagcg cggggcggcc atggcggaga aactcggttt
     2161 cgaggtgcaa tacctgtcgc ggtggcttcc aaagaagctc gacgaggccg ccacccgcga
     2221 gcttgtgaga gttgcgatcg ccgagctcgg tgtggccggt gatttgaagg ccaccgggag
     2281 agtcatcgga caggtgatga aggccgacgg cgagaacctc gacggcggac tggttagtcg
     2341 cgtcgttcgt gaggagttag cccccgattg agcgttcccg acttcgaacc gatgctggca
     2401 acccgttggc cagcggcgtt cgacgacccg ggatggtggt tcgaggtgaa gtgggatggc
     2461 taccgggcca tcgtctcggc cgagggcggg cgggtcaaag cccgttcccg tcggggcctg
     2521 gacctgatcg ggccctttcc tgagttggca ggcctcccca tccccgacgg cgtggcgctg
     2581 gacggtgagg tggtggcatt cgatgaggat gggcggcctt cgttttccaa ccttcagcag
     2641 cgcaccggtt tcgggggaaa gggcacgaga gccgacgtcg gggtgaatct cgttgtcttc
     2701 gacgtcctct tcgccggaga gcccatcatt ggtgagcctt atgaggagcg acgggcacaa
     2761 ctagagcgac tcgctctgcc cgccccaatc gtggttcccg agccccttcc cgaacacgga
     2821 ctgtcgcttt tcgcagctgc cgaaggtcag ggcttggagg gaatcgtcgc caagagagcg
     2881 ggctctccct atctgcctgg gaggagatcc cccgattggc ggaaggtcgc gatcaggcgc
     2941 cgcttgcggg cggttgttgg gggctatgtg cccggcgagc ggagccgggc gtccaccttc
     3001 ggctcgatcc tcgtgggcct caacgacgat cagggtctcc gctggatcgc agccgtcggg
     3061 tcgggtttcg acgatcaggc cctcgacgca ttttcgaggg cgctacgtga actggaaagg
     3121 ccgaccaagc cgttttacaa cgaggtgttg gtccccaatc ccaggccccc ggtgtgggta
     3181 gagccgggga tcgtggtcga tgtcgaattc aaagagtgga cccacgacct ccatctcaga
     3241 gcaccggtct acaaggggat cgagatcgcc gatccggact ccgtgacctg ggccgaggag
     3301 ggaccgggct agccggcttt ggcggttttc acttctttgg acttggtggc ttccaccgag
     3361 gccttgaggg cttcgaggag gtcgacgacc tttgtcggct ccggcgacgc aacggcgaca
     3421 atctcttccc cggctacttt cttggtggcc aggtcttcga cggcctcgcg ggtgggatcg
     3481 acataggccg cggcatcgaa gggcctggtc aggttgtcga tcagggaggc ggccattgcc
     3541 acttcttcgg gcctgatctc cacatcggcc gagagttcct cgaagtcggc ctcgcgcagc
     3601 tcgtcaggcc agtacatcgt ctccatgacg atggtgccgt ccttggctcg cag
//
LOCUS       DBNHXU010000159        11843 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1637276, whole genome shotgun sequence.
ACCESSION   DBNHXU010000159 DBNHXU010000000
VERSION     DBNHXU010000159.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 11843)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11843)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11843
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1637276"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..542)
                     /locus_tag="AC161X_04080"
     CDS             complement(<1..542)
                     /locus_tag="AC161X_04080"
                     /EC_number="2.5.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010468802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin synthase"
                     /protein_id="HIR4988758.1"
                     /translation="MFTGIVAHKGSVIDVEDDAGVRRLRVEAGSLADLAVGDSIAVNG
                     VCLTAVAVQGSTIDLEVVHETLSRSSLGAIQPGDPVNLERPMSAQGRFDGHIVQGHVD
                     GVGTVIGVADDGEERRLTVEVPAGMARFIVEKGSITVDGVSLTVSAVGSDRFEVALIP
                     HTLEVTTLGLRVEGDPVNLEV"
     gene            complement(543..1586)
                     /gene="ribD"
                     /locus_tag="AC161X_04085"
     CDS             complement(543..1586)
                     /gene="ribD"
                     /locus_tag="AC161X_04085"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: protein motif:HMM:TIGR00326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HIR4988759.1"
                     /translation="MGDARHMRRAIELALQHRTHPNPRVGAVIVDQAGNVVGEGAHEG
                     VGQPHAEVVAIDQAGALARGSTLYVTLEPCSHYGNTPPCVTAIVTAGISRVVVGAIDP
                     DERVSGKGVSWLTDARIDVVLEESAAEAESVDPAYFHHRRTGLPLVTWKTAMTLDGSV
                     AALDGTSQWISSEESRIDAHQLRAAMDAVVVGAGTLRADDPELTVRHLDGGDHQPRPV
                     ILAGAGPLPVDARIWDRSPLVVASRDLEIPSGDLVVVTGVDDRPDPVEAAKAIADAGL
                     LEVLLEGGPGVAGAWWRAGVISRGILYLAGKVGGGAGIGPLAGPFSTIDEAREVNIRD
                     VRNVGSDLRIEFE"
     gene            complement(1587..2516)
                     /locus_tag="AC161X_04090"
     CDS             complement(1587..2516)
                     /locus_tag="AC161X_04090"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR4988760.1"
                     /translation="MSERVLIADDDPDILAVVQVNFELEGLEVETAVDGEDALQKATA
                     NPPNVIVLDIMMPRMDGLTALHRLRSQASTGNIPIILLTARGLPEDRVRGLELGADDY
                     ITKPFDINELVARVRAVVRRTQAARDLSPLTGLPGNFKITAEIEERITSGRDFALVHG
                     DLDNFKAFNDHYGFMRGDEVIRFCAKGFRDASENLGITGAFVGHIRGDDFVAIIPPEM
                     AESFCKEVIERFDDGILDLYDTADALRGYIEVIDRRGERFAFPVVSLSLGVASTDVRE
                     ISTQWEASAIAVEMKEFAKKQPGSTYRIDRRSA"
     gene            complement(2513..3175)
                     /gene="rpe"
                     /locus_tag="AC161X_04095"
     CDS             complement(2513..3175)
                     /gene="rpe"
                     /locus_tag="AC161X_04095"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003725651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HIR4988761.1"
                     /translation="MRAVKIAPSLLAADFGCLRDEISSVESVADWLHIDVMDGHFVPN
                     VSFGIPVIKSIRPLTELRFDCHLMTTNPDAFLPDLAAAGVDLVTMHIEAMPDPTHASK
                     QARDNGLDFGLVCNPGTPFAGVAPFLELCDVLLIMSVEPGFGGQSFIPEVLEKIQAAR
                     EWVEERGLPTDIEIDGGITPETARMAADAGANVFVAGTAIFGAKDPSTAVAELRRAVE
                     EK"
     gene            complement(3202..4377)
                     /locus_tag="AC161X_04100"
     CDS             complement(3202..4377)
                     /locus_tag="AC161X_04100"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013362.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HIR4988762.1"
                     /translation="MSDPLQARHVAAEVVGRVLREGAFSNVLLDSHASSLPPRDLARV
                     KALTYGVLRHTERIDHAIEVGARRTIAKVDTGVLDGLRVSVFELLFSDLARPIVVSAG
                     VDLIRETRPKAAGFANAVLRRVAEQDREADQGLDFPAWLTRSLETAWGAGPAREFAAA
                     SATEPERVVRAMAGAVDVGEELAGIPGAFVVAPGPIPEGTTVQDAASIAVGNAVAAEP
                     GMRVIDLAAAPGGKTAHLLDQVGEGGRVVAADSHRRRVLDARSRVPAAKWVVADARAL
                     PFKRATFDRVLLDAPCSGLGTLRRRPEIRLRVTESEVLRLAAGQREMLERATELLAPG
                     GRLVYSVCTVTPEETIDIVAGRGFEPPPIPGEVWGDGRLLAPHITGTDGMFVALLEG"
     gene            complement(4374..5288)
                     /gene="fmt"
                     /locus_tag="AC161X_04105"
     CDS             complement(4374..5288)
                     /gene="fmt"
                     /locus_tag="AC161X_04105"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR4988763.1"
                     /translation="MTRIAFLGTPGAAIPTLDRLVSDFDVALVITQPDRPKGRSGRPE
                     PPPVKTRADELGLAVAQPSGSSEIEGMLVEAGPFDVGVVVAFGMLIKPGALAQPARGM
                     LNVHFSLLPRWRGAAPVARALMAGDPMIGVTIIELDENLDTGPVLTAQAVDIQREENA
                     GELTDRLAGLGGNLLANVLPAYLIGELVPVPQVEAGATYASKLTPDDRPIRPEMSSLD
                     AINRIRGLSPLPGATLDLDGEPHKILRARASEHSPEPGVYKVIDGIPVAGIADGGVEL
                     VEIQPPGGRAMSGAEWARGRRSQTGPFR"
     gene            complement(5285..5794)
                     /gene="def"
                     /locus_tag="AC161X_04110"
     CDS             complement(5285..5794)
                     /gene="def"
                     /locus_tag="AC161X_04110"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007638636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HIR4988764.1"
                     /translation="MAILPIRTFGDPVLRLETKTVLEVDDALRALAVDMIETMYQAPG
                     VGLAANQIGITRRIAVFDAQDGLGPRVMINPEIVELSGEWAYEEGCLSVPGHYWEVMR
                     PGFARARATDLDGKEVEFAGDGLLGRVLQHEIDHLDGKLIIDRLERRTRKQALRDLRD
                     EALGMASHE"
     gene            5910..6341
                     /locus_tag="AC161X_04115"
     CDS             5910..6341
                     /locus_tag="AC161X_04115"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HIR4988765.1"
                     /translation="MHITADDLIATLRDRGLRITDARRAVCGVIAHSHGDHLDAATIH
                     NKAMTDLGTDLDLSTVYRTLEVLEEAGAVRHAHLGEAAVYHLAEEQPHQHLVCRLCGS
                     TTALPASDLVGFLTSVEQLTGFVADVEHFALWGVCSNCSSV"
     gene            6390..7067
                     /locus_tag="AC161X_04120"
     CDS             6390..7067
                     /locus_tag="AC161X_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006538859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0000041 - transition metal ion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter permease"
                     /protein_id="HIR4988766.1"
                     /translation="MHIPDGFIGPGVSAAAGITALGGFGLALRRARDYLTDRLVPMAA
                     LVAAFVFAAQMINFPVLPGVSGHLLGGVLAAVLVGPWAGYIVISLVLLVQSLYADGGL
                     TALGLNIVNMALIGAVGGYLIYRALFRLGGSQPSTVPLFAGLAAFLSVPLAAMGFVVE
                     FALGGTVASVSIGSVFWAMLGTHVLIGIGEGLITFLVVGAVMRSRPDLVYGSPTFGGM
                     RTEELVA"
     gene            7064..7360
                     /locus_tag="AC161X_04125"
     CDS             7064..7360
                     /locus_tag="AC161X_04125"
                     /inference="COORDINATES: protein motif:HMM:NF024588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDGLE domain-containing protein"
                     /protein_id="HIR4988767.1"
                     /translation="MRKGLALFGIGLLISLLIATVISQFASSQPDGLNYVAEQQGFGN
                     AAEDHALDASPLAGYGGDSPARKAAAGAVGVLATLGLGYLVFYLVKSDKSTAEE"
     gene            7357..8121
                     /gene="cbiQ"
                     /locus_tag="AC161X_04130"
     CDS             7357..8121
                     /gene="cbiQ"
                     /locus_tag="AC161X_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012851719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0032778 - P-type cobalt transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt ECF transporter T component CbiQ"
                     /protein_id="HIR4988768.1"
                     /translation="MSGSHGHALFVHGHSPLHRAAPQVKIASALLMLFAIISTPRQAF
                     WAFGVYALALGTLVAIARIPIRFLLRRMLVLTPFVLLAVLLPVFGRGPRVEVVGLSLS
                     EQGLWDMWNLLAKASLGLVTSLLLGATTEVADLLRGFDSLRVPRLVTAILGFMVRYID
                     VVVSDFNRMRLALAARGHEASGLTQWAPYGRAMGTMFIRTYERGERVYLAMESRGYAG
                     EMPVSSRIKATSLEWALAAVLVTTFWTVAITARGTL"
     gene            8118..8888
                     /locus_tag="AC161X_04135"
     CDS             8118..8888
                     /locus_tag="AC161X_04135"
                     /EC_number="3.6.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007929812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR4988769.1"
                     /translation="MTTAVIEILGLGFSYPDGTEALTDIDLHIHRGERVALLGPNGAG
                     KTTLILHMNGIHRAQTGSVTVSGLPVTAPNLLEIRRRVGLVFQDPDDQLFMPTVREDV
                     EFGPRNLGLSGAELEERVNFALASLEVLDLADRAPNHLSFGQKRRVSMAGVMAMEPEI
                     LVLDEPTSNLDPASRQDLTRSLLGLDVTQLIVTHDLLYALEVCPRSVIIDSGRIVADG
                     ATKEILADDDLLAAHRLALPAAIHLLTGSGSAPDPTIN"
     gene            complement(8881..9006)
                     /locus_tag="AC161X_04140"
     CDS             complement(8881..9006)
                     /locus_tag="AC161X_04140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988770.1"
                     /translation="MASKDKGVKTTKKVASKDLKQKRQAKKDKKAAANSQTHKIN"
     gene            complement(9086..10864)
                     /locus_tag="AC161X_04145"
     CDS             complement(9086..10864)
                     /locus_tag="AC161X_04145"
                     /inference="COORDINATES: protein motif:HMM:NF036997.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N' family DNA-binding
                     protein"
                     /protein_id="HIR4988771.1"
                     /translation="MSRPAARVVPSIPTFAVDKGFWYSIPPQLEDLIQVGSIVRAPLG
                     GRKVRGYVVEMSPAREGQLKDLATVSGTGPVFDVDLLKSLEWAAIHYVAPISILLDRS
                     APPNLPKNEAATVESTVVGHPSEHPLYAAADAAAGGKRRPISAIVIGAQSTDWIESLA
                     PVLAAGKSALVVAATAQEVEDLVRPAAAVFGDRVVAVAGDTAHELTSAWEAAQGGGRL
                     VIATPRITTWMVAGLALVVVLEEGRRAMKDRQTPTIHVREMLTTRSRVEGFGLVFMGP
                     TPSIEVIAAGSEVVKGSSRSWPLVEIVDRREDSPGSGFLSERTIAALRATASTGRGAF
                     VFTHIRAADASMRCTQCRRVRNCEQCGSRLGRVEACRRCGAPAGKCPTCGSTSFEEMG
                     SVPERLSGEIDRRLGQGMSGVHPTDRLIAVGTERDLSGLPPVGLVVAADSDSLMLGHN
                     YRSGEEALRVMARLANKLEIGPGRRMMVQTSLPESSLMTTLRRGDPIPYLETMLSERV
                     QAGLPPASEMIALELRGEGSVGDADADIRSLGADTVLGPASANDRSRWLLQGVLGPVR
                     LSLRPLVQAWRERGWTVRIDADPIDL"
     gene            complement(10861..>11843)
                     /gene="metK"
                     /locus_tag="AC161X_04150"
     CDS             complement(10861..>11843)
                     /gene="metK"
                     /locus_tag="AC161X_04150"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012803232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HIR4988772.1"
                     /translation="GGEITAGVPLDVEGIVRDTVSRIGYDGFDPDFSSDSLEVQNRVH
                     EQSADIAAGVDRDLGAGDQGLMFGFAVDETEELMPLPIQLAHRLAERLSEVRRTGKLD
                     YLRPDGKTQVMIRYEDTRPVAVEKVLISAHHAPDKDQAEMAEEVFETVVAAVVSDEML
                     TPELEFIFNPSGRFVIGGPVADTGLTGRKIIVDTYGGYARHGGGAFSGKDSTKVDRSA
                     AYAARWAAKNLVAAGVASKLEIQLSYAIGRAEPFSIHVETFGTEMLSPEAISRVVDEV
                     FDFRPAAVISQLELTKPVFAQTAAYGHFGRPGFSWERLDRVDAIKAAAGL"
BASE COUNT         2072 a         3943 c         3649 g         2179 t
ORIGIN      
        1 acctcgaggt tgaccggatc gccttcgacc cgcaagccca gcgtcgtcac ctcgagggtg
       61 tgcggtatga gcgccacctc gaaacgatcc gacccaacgg ccgagacggt gagactgacc
      121 ccgtcgacgg tgatcgaccc cttctcgacg atgaaacggg ccatcccggc cgggacttcg
      181 acggttaggc gccgctcctc gccatcgtcg gctactccga tgacggtccc aacaccgtcg
      241 acatggccct gaacgatgtg cccgtcgaag cgaccctgag cggacatggg ccgctcgaga
      301 ttcaccgggt ccccgggttg gatcgcgccg aggctggaac gggacaacgt ctcgtggact
      361 acttcgaggt cgatcgtcga gccctgaacc gccacagccg tgaggcaaac cccgttgacc
      421 gcgatcgagt cgccgacagc caggtcggcg agggaccccg cctcaaccct gagccgcctt
      481 accccggcgt catcttcgac gtcgattacc gagcctttgt gggcaacgat tccggtgaac
      541 atctactcga actcgattct caggtccgag ccaacgttac gcacgtcgcg aatattcacc
      601 tctctggcct cgtcgatcgt cgagaagggg cctgccaacg gcccaatccc ggcccctcca
      661 ccgactttcc ctgccagata gagaatgcca cgggaaatca cgcctgcgcg ccaccaagca
      721 ccggccacgc cgggccctcc ctcgagcagg acttcgagca ggcccgcgtc ggcgatcgcc
      781 ttagccgcct cgaccggatc cgggcgatcg tcgacaccgg tgaccacaac cagatctcct
      841 gagggaatct cgaggtcgcg ggaggccacg accagcggcg agcggtccca gatcctggcg
      901 tcgacaggca acgggccggc cccggccagg atcaccgggc ggggttgatg gtctccgccg
      961 tcgagatgcc ggactgtcag ttcggggtca tccgctcgca atgtccccgc cccaactacc
     1021 acggcgtcca tcgcggctct cagctgatgg gcgtcaatgc gtgattcttc agagctgatc
     1081 cattgcgacg taccgtcgag ggccgccacc gagccgtcga gggtcatcgc tgtcttccag
     1141 gtgacgagtg ggagcccggt gcgccggtga tggaaatagg ccgggtccac agattcggcc
     1201 tcggcggccg actcctcgag aacgacgtcg atcctggcgt ctgtgagcca ggagacacct
     1261 ttgccggaaa cacgttcgtc tggatcgatg gcacctacca ccacccggct gatcccggcg
     1321 gtcacgattg cggtgacaca tggcggggtg ttcccgtaat gactacaagg ctcgagggtc
     1381 acatagagag tcgagccccg agctagggcc ccggcctggt caatcgcaac gacttcggcg
     1441 tggggctggc ccactccctc gtgggcgccc tctccaacca cgttgccggc ttgatcgacg
     1501 atgaccgcgc caacgcgtgg attgggatga gtccgatgct gcaaggcgag ctcgatggct
     1561 cgccgcatgt gacgggcgtc gcccacctat gccgaacgac gatcgatgcg gtaggtagag
     1621 cccggctgct tcttggcaaa ttccttcatt tccaccgcaa tcgccgacgc ctcccactga
     1681 gtggagatct ctctcacgtc ggtggacgct acacccagcg acagggagac cacagggaag
     1741 gcgaaacgct cgccgcgccg gtcgatcacc tcgatgtacc cgcgcagagc gtcggcggtg
     1801 tcatagaggt caaggatccc gtcatcgaat cgctcgatca cttccttgca gaacgattcg
     1861 gccatctccg gcgggatgat ggcaacaaag tcgtcgcctc tgatgtgacc cacgaatgcc
     1921 ccggtgatcc cgagattctc ggatgcgtcg cggaagccct tggcgcagaa gcggatcact
     1981 tcgtcgccac gcatgaaccc gtagtgatcg ttgaaagcct tgaagttgtc gaggtcacca
     2041 tggaccaggg caaagtcacg tccggatgtg atccgttcct cgatctcagc agtgatcttg
     2101 aagttcccgg gaaggccggt gagcggcgag aggtctcggg ctgcctgtgt ccggcgcacc
     2161 acggcccgga cccgggccac cagctcgttg atgtcgaagg gcttggtgat gtagtcgtcg
     2221 gctcccaact cgagaccacg cacccggtct tctggaaggc cgcgggcggt gagcaggatg
     2281 atcgggatgt tgccggtgct tgcctggctg cgtaggcgat gcagggcggt caagccgtcc
     2341 atccggggca tcatgatgtc gagaacgatc acattcggcg ggttggcggt cgccttctgg
     2401 agagcatcct ctccatcgac cgcggtttcc acctcgagcc cctccaactc gaaattgacc
     2461 tggacaaccg caaggatgtc cgggtcatcg tccgcaatca ggacccgttc gctcatttct
     2521 cctctactgc ccttctcaat tcggccacag ccgtgctggg atctttcgcc ccgaaaatcg
     2581 cggttccggc cacgaacacg ttggcacctg catcagcggc cattcgtgcg gtttcgggcg
     2641 taataccccc gtcaatctca atatcggtcg gcaatccgcg ctcctcaacc cattctctgg
     2701 cggcctggat cttctcgaga acctccggaa tgaagctctg gcctccaaaa ccaggctcga
     2761 ccgacatgat cagaagaacg tcgcagagct cgagaaatgg ggccacaccc gcaaaagggg
     2821 tgcccggatt gcacaccagc ccgaaatcca gtccgttgtc acgcgcttgc tttgaggcgt
     2881 gggtgggatc tggcatcgcc tcgatgtgca tggtcaccag gtcgactccg gccgccgcca
     2941 gatcggggag aaaggcgtcg ggattcgtgg tcatcaggtg acagtcgaac cggagctcgg
     3001 tcaatggtcg gatgctcttg atcaccggga ttccgaagga aacattgggg acgaagtgcc
     3061 cgtccatcac atcgatgtga agccaatccg cgaccgactc cacgctcgaa atctcgtctc
     3121 ggagacagcc gaaatcggca gccagcaaag aaggtgcgat cttgacggcg cgcatccaat
     3181 gggattgtaa gggggggaac ctcaaccctc gagtaaggcg acaaacatgc cgtcggtgcc
     3241 ggtgatgtgc ggtgcgagga gcctgccgtc accccagacc tcgcccggta tcggcggggg
     3301 ctcgaaacca cgcccggcga cgatgtcgat cgtctcctca ggagtgaccg tgcacaccga
     3361 gtagaccagg cggccacctg gggccagcaa ctccgtggcc cgctccagca tctcccgctg
     3421 accagctgcg agccgcagga cctcggactc ggtaacacgc agcctgatct ccggacggcg
     3481 cctcagggtg ccgaggccgc tacacggcgc gtcgagtaga acgcggtcga atgtggcgcg
     3541 tttgaacggc agcgccctgg cgtcagccac cacccacttc gctgccggga cccgggagcg
     3601 cgcatctaag acccgccgcc ggtgggagtc ggcagccaca acccggccgc cctcccccac
     3661 ctgatcgagg agatgagccg tcttgccacc cggggcggct gccagatcga tcacccgcat
     3721 cccgggctca gccgccaccg catttccaac cgcaatggat gctgcgtcct ggacagtcgt
     3781 tccctccggg atcggccccg gcgcgaccac aaaggcgccg ggaatgccgg ctagctcttc
     3841 gccgacatca accgcgccgg ccatcgcccg aaccacacgc tcgggctcgg tcgcgctcgc
     3901 cgcggcgaac tctctggccg ggccggcacc ccacgcagtc tccagtgagc gagtcagcca
     3961 ggcagggaag tccaaaccct gatcggcctc acggtcttgt tcggcaaccc tcctcaggac
     4021 ggcgttggca aacccggctg ctttgggccg ggtctcccgg atcagatcga cgccagcact
     4081 caccacgatc gggcgggcca ggtcggaaaa gagcagttcg aataccgaga cccgcaggcc
     4141 gtcgagcacg ccggtatcga ccttcgcgat cgtccgccgc gctccgacct cgatggcatg
     4201 atcgatgcgc tcggtgtgtc gcaacacccc gtaggtgaga gccttgaccc gagccagatc
     4261 tctcggcggg aggctcgagg catggctgtc gaggagcacg ttggaaaagg ctccttcccg
     4321 cagcaccctg cccacgactt ccgcggccac atgccgggcc tggagcgggt cactcatcga
     4381 aatgggccgg tttggctgcg tcgacctctc gcccactcgg cgcctgacat cgcacggcct
     4441 cccggaggct gaatctcgac cagttccact cccccgtctg cgattcctgc caccggaatc
     4501 ccatcgatca ccttgtagac accgggctcg gggctgtgct cggatgctcg cgcccggaga
     4561 atcttgtggg gctccccgtc gagatccagg gttgccccgg gcaaaggcga caagccccgg
     4621 atccggttga tcgcatccaa cgacgacatt tccggccgga tcggacggtc atcgggagtt
     4681 agcttcgagg cgtaggtcgc ccctgcttcg acctggggaa cgggaaccag ctctccaatg
     4741 agataggccg gaagcacgtt tgccaacaga ttcccgccaa gtcccgccag ccggtcggtc
     4801 agctcaccgg cattctcttc acgctggatg tcaactgcct gggcggtcag caccggaccg
     4861 gtatccaggt tctcgtcgag ctcgatgatg gtcaccccga tcatcggatc ccccgccatc
     4921 agtgctcgag ccacgggtgc ggccccccgc caacggggca gcagggagaa gtggacgttc
     4981 aacatgcctc gagctggttg ggccaaggcc ccaggtttga tcagcatccc gaaggcgacc
     5041 accacgccaa cgtcgaatgg cccggcttcc acaagcatgc cttctatctc gctcgagccc
     5101 gaaggctggg ccacggccag gccgagctcg tcggcccgag tcttgaccgg cgggggctcg
     5161 gggcgaccag acctgccctt ggggcggtct ggctgggtga tcacaagcgc cacgtcgaag
     5221 tcgctgacga ggcgatcgag cgtgggtatg gcagctcccg gggtccccag gaacgcgatc
     5281 cgagtcattc gtgactcgcc atccccagtg cctcgtccct caaatcgcgg agcgcctgct
     5341 tgcgggttcg tcgttccaat cgatcgatga tgagcttgcc gtcgagatgg tcgatctcat
     5401 gctgaagcac tcgtcccaga agcccgtcgc cggcgaactc gacctccttg ccgtcgaggt
     5461 cggtggctcg ggctctggca aagccgggcc gcatgacctc ccagtagtgg cctggcacgg
     5521 agagacaccc ctcctcgtat gcccattccc cgctgagctc gacgatctcg gggttgatca
     5581 tcaccctcgg gccgaggccg tcctgggcat cgaataccgc tatccgacgc gtgattccga
     5641 tctgattggc ggccaacccg accccgggcg cctgatacat ggtctcgatc atgtccactg
     5701 ccagggcgcg cagtgcatca tcgacttcga gaaccgtttt ggtctcgagg cggagcaccg
     5761 ggtctccaaa ggttcggatc ggaagaattg ccatgactgt ccgggacggg gaaaggtggc
     5821 agggtagacg aaacgggctt ctcccagctt ctgaccgatt cccgttagat tcgcccgatg
     5881 acgcccgtcg gcctgggaac cctccgatca tgcacatcac tgctgacgac ttgattgcca
     5941 ccctgcgcga tcgtggcttg cgaatcaccg acgcccggcg agccgtttgc ggggttatcg
     6001 cccactctca cggtgaccac ctcgatgccg ccaccatcca caacaaggcg atgaccgacc
     6061 tcggaaccga cctcgacctg tcgacggtct atcggacttt ggaggtcctc gaggaggctg
     6121 gagcggtgcg tcatgcccac ctcggcgaag ctgccgtgta ccacctggct gaggaacagc
     6181 cccaccaaca cctggtctgc cgcctctgtg gctcgaccac tgccctcccg gcgtctgatc
     6241 tggtcgggtt tttgacctcg gtggagcaat tgaccggctt cgtggccgat gtcgagcact
     6301 tcgcactctg gggcgtttgc tccaactgca gcagcgtctg acagtcgcta ttctcttgtt
     6361 gcaagtcctt cgcaactagg agacacccga tgcacatccc ggacggcttt atcggaccgg
     6421 gtgtttccgc ggccgctggg atcaccgccc tcggaggttt cgggctggca ctccgcaggg
     6481 cccgtgacta tctcaccgac aggttggtcc cgatggcggc tctcgtcgcc gccttcgtct
     6541 ttgcggccca aatgatcaac ttcccggtgc tgcccggagt gagcgggcac ttgctcggcg
     6601 gcgtgctggc tgcagtcctg gtcggcccct gggccgggta catcgtgatc tccctggtct
     6661 tgttggttca atcgctctat gccgatggcg gactgaccgc actcgggctc aacatcgtga
     6721 acatggccct gatcggggcg gtgggcggct acctcatcta ccgggcgtta tttcgcctcg
     6781 gcggctcgca gccatcgacc gttcccctct ttgccgggct ggcagccttt ctctcggtcc
     6841 cactggccgc gatgggcttc gtcgtcgaat ttgcactcgg aggaaccgtg gcgagcgtct
     6901 cgatcggttc ggtgttctgg gcgatgcttg gcacccatgt cctgatcggg atcggggagg
     6961 gtctgatcac cttcctggtg gtgggcgccg tcatgcgctc ccgtcccgat ttggtctatg
     7021 gatcgccaac ttttggcggg atgcggaccg aggaactggt ggcatgagga agggtcttgc
     7081 tctgtttggg atcggtctac tcatctccct gctgatcgcc accgttattt cccagttcgc
     7141 ctcgagccag cccgacggcc tcaactacgt tgccgagcag cagggctttg gaaacgcggc
     7201 cgaagaccac gcattggacg cctcaccgct ggccggatat ggcggcgaca gccccgcccg
     7261 caaggcggca gccggggcgg tcggtgtgct cgccacgctc ggtctcggtt acctcgtgtt
     7321 ctaccttgtc aagtcggaca agagcaccgc ggaggagtga gcggctcaca cggccacgcc
     7381 ctgttcgtgc acggccactc cccgttgcat cgagcggctc cccaggtgaa gatcgcgtct
     7441 gccctgctga tgctcttcgc catcatcagt actcctcgtc aggccttctg ggcattcggg
     7501 gtctacgcac tggccctggg cacgctggtg gcgatcgctc ggatcccgat ccgatttctg
     7561 ctgcgccgga tgctggtgct caccccattt gtcctcctag cggtcctgct cccggtgttc
     7621 ggaagggggc cccgcgtcga agtggtcggt ctttctttgt cagagcaggg cttgtgggac
     7681 atgtggaatt tgctcgcgaa agcaagcctc gggttggtca cttcactcct gttgggtgca
     7741 accaccgagg tcgccgacct gctgagaggg ttcgactccc tgcgggtgcc cagactggtg
     7801 acggccatac tcgggttcat ggtccgctac atcgatgtgg tggtcagcga cttcaaccgg
     7861 atgcggctgg cgcttgccgc ccgcgggcac gaagcatcag gcctcaccca gtgggcgccc
     7921 tatggacggg ccatgggaac gatgttcatc cgaacctacg aacgcggcga gcgggtctat
     7981 ctggcgatgg agtctcgtgg ctacgcaggc gagatgccgg tttcctcgcg aatcaaagcc
     8041 acttccctgg aatgggccct cgccgcggtg ctggttacca ctttctggac ggtggccatc
     8101 acagctcgag gaacgctatg accacagcgg tcatcgagat ccttgggctc ggcttctcct
     8161 atcccgatgg aaccgaggcc ctgacagaca tcgacctcca catccaccgt ggggagcggg
     8221 tggcgctgct cggtcccaac ggggcgggca agacgacgct gatcctgcac atgaatggga
     8281 tccatcgcgc ccagaccgga tcggtcaccg tgtcagggct tccagtcacg gcaccaaatc
     8341 tcctcgagat ccggcgccgg gtcggcctcg tcttccagga tccggatgac cagttgttca
     8401 tgccgaccgt tcgtgaggac gtcgaattcg ggccgagaaa cctcggactg tccggagcgg
     8461 agctggagga gcgcgtcaac ttcgccttgg cttcgctgga agttctcgat ctcgccgacc
     8521 gggcccccaa ccacctgtca ttcgggcaga agcggagagt ttcgatggca ggagtgatgg
     8581 ctatggagcc tgagatcctg gtcctcgacg agcccacgtc caatctcgac ccggccagtc
     8641 gtcaggactt gacccgttct ttgctcggcc tggacgtcac ccagctgatc gtcacccacg
     8701 accttctcta tgcgctcgag gtgtgcccgc gatcggtgat catcgactcg gggaggatcg
     8761 tcgctgacgg tgcgaccaag gaaatcctcg ccgacgacga tctgttggca gcgcatcggc
     8821 ttgccttgcc cgctgcgatt cacctactca ccggctcggg gagcgcgccg gacccgacga
     8881 tcaattgatc ttgtgggtct gactgttggc ggcggccttc ttgtccttct tggcctgccg
     8941 cttctgcttc agatccttcg atgcgacctt cttggtggtc ttgacgcctt tgtctttgct
     9001 tgccatgggt gcctctcgcg ctggaagaat cgaatcccca ttctgacagg ctccttgccc
     9061 cagaactcgg tgaatactcg aatcctcaga ggtcgatggg atcggcgtcg atccgcacgg
     9121 tccatccccg ctcccgccag gcttgaacga gggggcgcaa ggagagtcga accgggccca
     9181 gcacaccttg gagtagccag cggctgcggt cattggcgct ggccgggcca agaaccgtgt
     9241 ccgcccccag ggaccggatg tcggcatcgg catccccgac cgagccctct ccccgcagct
     9301 caagggcgat catctccgac gccggtggca agcctgcctg cacccgttcc gacagcatgg
     9361 tctcgaggta tgggattgga tcccctcgcc gcagagtggt catcagcgac gactcgggga
     9421 gcgaggtctg caccatcatg cgccgtccag ggccaatttc gagtttgttg gccagccggg
     9481 ccatgacccg aagcgcctcc tctccagagc gatagttgtg cccgagcatc agggaatcgg
     9541 aatcggcggc gacgaccaac ccgaccggcg gaagaccgga caggtcgcgc tcggtgccca
     9601 cggcaatcaa tcggtcggtg ggatggacgc cagacatacc ctggccgagt cggcgatcga
     9661 tctctcccga caggcgctcc ggcaccgagc ccatctcctc gaacgaggtg gatccacagg
     9721 tggggcactt tcccgccggc gcaccacagc gtcggcacgc ttcgacccgc cccagacgtg
     9781 agccgcattg ctcgcaattg cgcacccttc ggcattgggt acagcgcatc gaggcatcgg
     9841 cggctcgaat atgggtgaag acgaatgccc cacgaccggt cgaagcggtc gcccgcaagg
     9901 cggcgatggt cctctccgac aggaaccccg agccgggtga gtcctctctg cggtcgacga
     9961 tctcgacgag tggccaggat cttgacgaac ccttcaccac ctcggacccg gcggcgatca
    10021 cctcgatcga aggagttggc cccatgaaca ccaggccaaa cccctccact cgtgaccttg
    10081 tggtcagcat ctcccgaaca tggatcgtgg gagtttgccg gtccttcatc gcacgccgcc
    10141 cctcttcgag cacaacgacc agtgccaggc cggcgaccat ccacgtcgtg atccggggcg
    10201 ttgctatgac caggcgcccg ccaccctgtg ccgcttccca ggccgacgtc aactcgtgag
    10261 cagtgtcgcc ggccaccgcc accactctgt ctccgaacac agccgccgcc ggccgaacaa
    10321 ggtcctcgac ttcctgggct gtcgccgcca cgaccagggc cgacttcccc gcagccaaca
    10381 ccggagccag ggactcgatc caatccgtgg attgggcacc gatcacgatg gcgctgattg
    10441 gtcggcgctt ccccccggcc gcagcatctg ccgcggcgta gagaggatgc tcagacgggt
    10501 gcccgacgac cgtggattcg acggtggcgg cctcgttctt ggggagattg ggaggagccg
    10561 agcgatcgag aaggatggag attggtgcca cgtagtggat cgcggcccat tccaggctct
    10621 tcagcagatc cacgtcgaat acgggccccg tgcccgagac cgtggcgagg tccttgagct
    10681 ggccttctct cgccggagac atctcgacca cgtagccacg gaccttgcga ccgcccaacg
    10741 gggctcgcac gatcgaaccg acctggatca ggtcttcgag ttggggaggg atcgaatacc
    10801 aaaatccctt gtctacggcg aaggtcggga tgctggggac aacccgggcc gcaggtcgac
    10861 tcagagaccg gccgccgctt tgatcgcgtc aacccggtcc agtcgctccc aggagaagcc
    10921 aggccgcccg aaatggccgt aggcggcagt ctgggcaaag acgggcttgg tgagctcgag
    10981 ttgggagatc accgctgccg gacggaagtc gaacacctcg tcgaccaccc gcgagatcgc
    11041 ctccggggac agcatttcgg tgccgaatgt ctcaacgtgg atggagaagg gctcggcccg
    11101 cccgatcgca tacgaaagct ggatctccag cttgctggcc actccggcag caaccaggtt
    11161 cttcgctgcc caccgggcgg cgtaagcggc cgagcgatcg accttcgtcg agtccttgcc
    11221 ggagaaggcg cctccaccgt gccgggcata gcccccgtag gtgtcgacga tgatcttgcg
    11281 cccggtcaac ccggtgtcgg ccaccggccc tccaatgacg aatctgcccg aagggttgaa
    11341 gatgaattcc agctcggggg tgagcatctc gtcggacacg acagccgcaa ccacggtctc
    11401 gaagacttcc tcggccatct cggcctggtc cttgtcgggg gcatgatgcg ctgagatgag
    11461 gaccttctcg acggcgaccg ggcgggtgtc ctcgtagcga atcatgacct gggtcttgcc
    11521 atcgggccgg agatagtcga gctttccggt tcgtctcact tccgaaaggc gctcagccag
    11581 gcggtgtgcc aactgaattg ggagcggcat caattcctcg gtctcatcga cggcgaaacc
    11641 gaacatcagc ccctggtcgc cggcgccgag gtcgcggtcg acccctgctg cgatgtcggc
    11701 actctgctcg tggacccggt tctggacttc gaggctgtct gacgagaaat caggatcgaa
    11761 accgtcatat ccgatgcggg atacggtgtc ccgcacgatt ccctcgacgt cgaggggaac
    11821 tcccgcggtg atctcgcctc caa
//
LOCUS       DBNHXU010000160         3045 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3225063, whole genome shotgun sequence.
ACCESSION   DBNHXU010000160 DBNHXU010000000
VERSION     DBNHXU010000160.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3045)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3045
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3225063"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..1216)
                     /locus_tag="AC161X_04155"
     CDS             complement(56..1216)
                     /locus_tag="AC161X_04155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR4988773.1"
                     /translation="MLDQETTQEITDPAEVVDPVLETASVTPPVIPATPPVIPAKPSQ
                     RRFWASLLSAVLLVVASSGITYLVVTDSTDTNVVAGTDQSASPSGDNPAVAIPADEPI
                     ANAAAIIIPSVVQIQTESGVGSGVIYDSDGLILTAAHVVSGEETVTVRLNDGEEMQGT
                     VLGGTSGADVAVVKVDRTGLPAATLALDSDAVVGQMAIAVGSPWGLQGTVTSGIISAV
                     NQSIPSGGTARSVLQTDAAINPGNSGGPLVDREGRVLGINVSIFSLSGANDGVGFAVP
                     IAVANDIAEQVVAGDSIETAFLGVSVGNVESGQAGALIAEVTPGSAADKAGIKVNDLV
                     TSIDGVQVQGGSDLAAQVQTHRPGTTVDLVVVRNGAEQTFSVTLDQRPDDLG"
     gene            complement(1283..2434)
                     /locus_tag="AC161X_04160"
     CDS             complement(1283..2434)
                     /locus_tag="AC161X_04160"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4988774.1"
                     /translation="MTIWRSIRFRLSVQYSAVVFGLGGALLGLVYLALRKMLHAQTIT
                     QYVVRGEPVVVNGVQVDVMPRLEAWELRTLESLFNELVLNQLARYTILSLIVLFLLSL
                     IVGWIMSGRVLRPVQEITEVASDIQATDLSRRIGLQGPDDELTRLAATFDSMLERLDR
                     AFSSQRKFLADTSHDLRTPLAVIRANVEVVADDENASIDDWKEVGGVVRRNVEKMSAM
                     IEGLLATARLQTGRAQAVTIDLAQLVTAKATEYENVAGEVGVSIDLAVTPAVIEGVEV
                     PLDRAFSNLLDNALGVSKPGDAIRVGAGVVSDWAWMGVEDQGPGLPESPESGRTGLGL
                     SIVTQIAEAHGGELASYPASGRAGTTMIVWLPVASATNPHPDASPLTEV"
     gene            complement(2431..>3045)
                     /locus_tag="AC161X_04165"
     CDS             complement(2431..>3045)
                     /locus_tag="AC161X_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020679123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR4988775.1"
                     /translation="RDGHAVDVALTAGDADFKVRTSGYDLICLDWNLPDGSGLALCSA
                     LVSGEVPTFEADRPRILMLTARDAVEDRVAGLDSGADDYLVKPFAFAELSARVRALLR
                     RDDTVDPVIEVGGLRLDPARFEATRDGRPLELTAKEFSLLHYFMAHPGSVMSQEHLLE
                     HVWDEMADPMTNVVRVTLSNLRKKLGPSQPITTVPGRGYRLEES"
BASE COUNT          602 a          946 c          958 g          539 t
ORIGIN      
        1 cgggcccggc aatggtagcc gggcccgcat tccctgattg cagtgaagga aagacttaac
       61 cgaggtcgtc gggcctctgg tcgagggtca cactgaatgt ttgctcagcg ccattgcgga
      121 caacgaccaa gtcgaccgtg gtcccggggc gatgggtctg cacctgagct gccaagtcag
      181 atccaccctg gacctgcacc ccatcgatcg aggtcacgag atcgttgacc ttgatcccgg
      241 ccttgtcagc tgcgctccca ggggtgactt cggcgataag ggctccggcc tggccggact
      301 cgacgtttcc gacgctcact ccgaggaagg cggtctcgat ggaatccccg gcaacgacct
      361 gctcagcgat gtcattggcg accgcgatcg gcaccgcgaa accaaccccg tcgttggcac
      421 cggagaggct gaatatcgag acgttgatcc caaggacccg tccctctcga tcgacgagcg
      481 gcccaccgga gttgcccggg ttgatcgcgg cgtcggtctg gagcacggag cgggcagtgc
      541 cacccgaagg gatcgactgg ttgacggcgg aaatgattcc ggaggtcacg gtgccctgga
      601 ggccccatgg gcttccgacg gcgatcgcca tctggccgac gaccgcatct gagtcgagag
      661 ccagcgtggc ggcaggaagg ccggtccgat ctaccttgac cacggcgaca tcggctccgg
      721 aggtcccgcc gaggacagtt ccctgcatct cttcgccatc attgaggcga accgtgaccg
      781 tttcctctcc gctgacgacg tgggccgcgg tgaggatcag gccgtcggag tcgtagatga
      841 cgcccgagcc gaccccactt tcggtctgaa tctggacaac tgacggaatg atgatcgccg
      901 cggcattcgc gatcggctca tcggctggga ttgccaccgc agggttgtca cctgatggcg
      961 atgcgctctg gtcggtgccg gctaccacgt tggtgtccgt ggagtcggtc acgaccaagt
     1021 aagtgatccc agacgaggcc accaccagaa ggaccgcgct gagcaggctg gcccaaaagc
     1081 gacgctgcga cggcttggcg gggataacag gtggagtggc aggaattacg ggcggtgtca
     1141 ccgatgccgt ctcgagtacc gggtcgacga cctcggccgg gtcggtgatt tcctgagtcg
     1201 tttcttgatc aagcattgct gtgcggggac ctcctgggag gtcacgctat tgggagcccc
     1261 ctaaacgacc ggtaaatggg ctctaaacct cggttaaagg tgaggcgtcg ggatgtgggt
     1321 tggtggctga tgccacgggg agccagacga tcatcgtggt gccggcccgc cccgaagccg
     1381 gataggaggc gagctcacct ccatgggcct ctgcaatctg ggtgacaatc gacaggccga
     1441 gcccggtgcg accggattca gggctttcgg gcaagcctgg gccctggtcc tcgacgccca
     1501 tccatgccca atccgaaacg accccggcgc caacgcgaat ggcatctccc ggcttggaca
     1561 ccccaagtgc gttgtcgagc aaattcgaaa acgcacggtc gagagggacc tcgacaccct
     1621 cgatcaccgc cggagtaact gccaagtcga tcgagacgcc gacctcgccc gcgacgttct
     1681 cgtattccgt ggccttggca gtgaccagtt gggccaggtc aatggtgacc gcctgggcac
     1741 gcccggtctg gagacgagcg gtcgccaaaa ggccttcgat catcgccgac atcttctcca
     1801 cgttcctccg gacgacgcca ccgacttcct tccagtcatc gatgctggcg ttctcgtcat
     1861 cggcgaccac ctcgacgttg gcgcggataa ccgccaacgg agtgcgcagg tcgtggctgg
     1921 tgtcggccag aaacttccgt tgactcgaga acgcccgatc gagcctctcc agcatgctgt
     1981 cgaaggtggc tgcgagacgg gtcaactcgt cgtcggggcc ttggaggccg atgcgacgac
     2041 tcaagtccgt ggcctgaatg tcagaggcga cctcggtgat ctcctggacc ggcctgagca
     2101 cccttcccga catgatccac cccacaatca ggctcaacag gaacagaacg atcagggaga
     2161 gaatggtgta acgggcgagt tgattgagaa ccagctcgtt gaacagggat tcgagcgtgc
     2221 gcaactccca ggcctcgagc cttggcatca cgtcaacctg caccccattt accacgaccg
     2281 gctcaccgcg caccacgtat tgggtgatgg tttgagcatg gagcatcttg cgaagggcga
     2341 gatagaccag ccccagcaag gcaccaccaa gcccgaaaac gaccgcggag tactggaccg
     2401 aaagccggaa tcgaattgat cgccagatag tcatgattcc tcgagccgat acccgcgccc
     2461 gggaaccgtg gtaatcggtt gcgatggtcc gagcttcttt cgaaggttgc tcaaggtgac
     2521 ccggaccaca ttggtcatcg gatcggccat ctcgtcccag acatgctcga ggaggtgctc
     2581 ctgactcatg actgagccgg gatgcgccat gaagtagtgg agtagggaga attccttggc
     2641 tgtgagttcc aagggccgcc cgtcacgggt tgcctcgaac ctcgccggat cgagtcgcaa
     2701 accgcctact tcgatcactg gatcgaccgt gtcgtcacgg cgcaacaatg cacgcacccg
     2761 tgccgagagc tccgcaaagg caaaaggttt aaccagataa tcgtcggcgc ctgagtccaa
     2821 acccgccacc cggtcctcga cggcgtccct ggccgtgagc atcaggattc gggggcgatc
     2881 agcctcgaat gtgggcactt cgcccgaaac caacgctgag cagagcgcca gaccggagcc
     2941 atctgggaga ttccagtcaa ggcagatcaa gtcgtaaccg gacgtgcgga ccttgaagtc
     3001 ggcgtcgcct gcggtgagcg cgacatcgac ggcgtgaccg tcgcg
//
LOCUS       DBNHXU010000161         3723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1837120, whole genome shotgun sequence.
ACCESSION   DBNHXU010000161 DBNHXU010000000
VERSION     DBNHXU010000161.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3723
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1837120"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /locus_tag="AC161X_04170"
     CDS             complement(<1..694)
                     /locus_tag="AC161X_04170"
                     /inference="COORDINATES: protein motif:HMM:NF017046.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P N-terminal domain-containing
                     protein"
                     /protein_id="HIR4988776.1"
                     /translation="MSDRFAARRRRLGEIVGVDGIAVIPAAVEVTRSYDTSFDFHQDP
                     DFLYLTGFPEPEAVAVITPGHSDGDYTLFVRARDPEMEAWNGYRAGVDGAKERFGVDA
                     AYELGELDEVVTRLMIGRDALWYETGNEHHDSRIERLVEKARDHRVRYGGSVPSAVRD
                     LSVVLGEMRLFKTDDEIASLRRACELTARGHIEAMRFARPGIYEYQVQAALEYFWRTG
                     GSRHNGYPSIVAS"
     gene            complement(714..1646)
                     /locus_tag="AC161X_04175"
     CDS             complement(714..1646)
                     /locus_tag="AC161X_04175"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015338.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HIR4988777.1"
                     /translation="MILVIIPAAVIAVASLASFAGRWVWWLDVLANFRAQYVVVLAIL
                     GGIVILSRWKKSGSAILAVCLVNLVFVAPLYFGSPGKSDPAAPHLSVLSFNLLSTNEN
                     YNEVIDYIDSVDADLVFLHEASRPWEVALEAADLDYEIIRARSDNLIFGTVILARDEV
                     EAVSHGFAEAQPRAVTLNYQPDGWPVSVQVLSSHTLAPTTEKRAGLRDAQLAFAASWA
                     AEQSGAYFVVGDLNATPWSWPIRNLVSEGRLRNSQIGFGLQPSFSATSFVLLRVPIDH
                     LLHSDALRVRDRRLGPNLGSDHFPLLVDLEYVGS"
     gene            complement(1654..3018)
                     /gene="argH"
                     /locus_tag="AC161X_04180"
     CDS             complement(1654..3018)
                     /gene="argH"
                     /locus_tag="AC161X_04180"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HIR4988778.1"
                     /translation="MTLWEGRFESAPGSILWDYTTDPSDRRLLLDDLDGSIAHVTMLG
                     TTGVISAVDSKEIVAGLETIRAEAEQGKFVFSESDEDVHSAVERRLGELIGAVAGKLH
                     TARSRNDQIALDLRLYLRRSATQRIEQLHEFALRLCDLGETAAAVPVPSYTHLQQAQP
                     STLGHHLLAYAWMALRSVDRFTGSRSRLDVSPLGAGASSGTSLGIDPEQSASLLGFGT
                     VFSNSLDAVGSRDHVAEYVFCCAQAMVDLSRLAEEIVLWTSTEFGWARLDDSVSTGSS
                     ALPHKRNPDIAELVRGRSARAAGDLAAVIALQKGLPLAYNRDLQEDKRLVFDADDVLE
                     SSLLAMTEMLGHIEFAPPMPEGATASLDLAEALVRRGIPFREAHKTVGMLIITLENAG
                     RSLTDATFDDLKTAHDSFEEADLAVVDVEKSVTGERSPGSGTAASVHRQVTAIRSRIG
                     ADAG"
     gene            complement(3015..>3723)
                     /locus_tag="AC161X_04185"
     CDS             complement(3015..>3723)
                     /locus_tag="AC161X_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011531187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenine nucleotide alpha hydrolase family
                     protein"
                     /protein_id="HIR4988779.1"
                     /translation="IAVPVTKEAPYSTDANLLHVSYEGGVLEDPWTAPPDKMFRLTTD
                     PADAPDAAEEVVVGFETGNPVSINGESLGPVDLLERANEIAGRNAVGRIDIVENRFVG
                     MKSRGVYETPGGTLLHLSHRAVESITLDRELMHLRDQLVPRYADMVYNGFWFSPERIG
                     LQSFMDEIQKPVTGEARVVLYKGSATVNGRRAPKSLYDAGSATFEADDVYRQADAEGF
                     INLNALRIRGFGDGSGG"
BASE COUNT          729 a         1197 c         1144 g          653 t
ORIGIN      
        1 cggaagcgac gatcgatggg tagccgttgt gcctcgaccc acccgtccgc cagaagtact
       61 ccagggcggc ctgcacctgg tactcgtaaa tgcccggtct cgcgaagcgc atggcctcga
      121 tatggccccg cgccgtcagc tcacaggccc gccgcaagct ggcgatctcg tcgtcagtct
      181 tgaacagcct catctctcca aggacgacgg agaggtcgcg gaccgcggag ggcaccgaac
      241 ccccgtagcg aacccggtgg tcgcgagcct tctcgacgag ccgctcgatt cttgagtcgt
      301 ggtgctcgtt gccggtctcg taccacaggg catcacgacc gatcatgagt cgggtgacca
      361 cctcgtccag ctcgcccagc tcataggcgg cgtccacccc gaacctctcc ttggccccgt
      421 cgaccccggc ccggtaaccg ttccaggcct ccatttcggg atctcgggcc ctcacgaaga
      481 gggtgtagtc cccatcggag tggccaggag tgatcactgc cacggcctcg ggctccggaa
      541 acccggtcag atagaggaaa tccgggtcct ggtggaagtc gaacgacgtg tcatagctgc
      601 gagtcacctc gacggcggcg gggatcaccg cgatcccatc gactccgacg atctcaccca
      661 gacgacggcg acgtgctgca aagcgatcag acatgcgtca aacggtagtt cgcctaggag
      721 cccacatact ccaggtccac caacagcggg aagtggtcag agccgagatt tggtcccagc
      781 cttcggtccc tgactctgag ggcgtcgcta tggaggagat gatcgatcgg caccctgagc
      841 aggacaaaag aggtcgccga gaacgaaggt tgcagcccga atccgatctg cgagttccgt
      901 aatcggccct cggagaccaa attgcggatc ggccatgacc agggagttgc gttgaggtcg
      961 ccgaccacga agtaggcgcc tgactgttcc gccgcccaac tcgcggcaaa agccagctga
     1021 gcgtcacgaa gcccggcgcg cttctctgtg gtcggagcca gggtgtggct ggagaggacc
     1081 tggaccgaga ccggccagcc gtcaggctga tagttgaggg tcaccgcacg aggttgagcc
     1141 tcggcgaagc catgcgaaac ggcttcgacc tcatcgcggg ccagaatcac cgtgccgaag
     1201 atgagattgt cggatcgggc cctgatgatc tcgtagtcga gatccgctgc ctcgagggcg
     1261 acctcccaag gtcggctcgc ctcgtgcaag aaaacgaggt cggcgtccac ggagtcgatg
     1321 tagtcgatga cctcgttgta gttctcgttg gtcgagagga ggttgaagct caacacagag
     1381 agatggggtg cggcaggatc cgacttcccc ggtgagccga agtagagagg tgccacgaaa
     1441 accaggttca ccaggcatac cgcgaggatc gcgctcccac tcttcttcca ccgtgacagg
     1501 atgacgatcc cgcccagaat cgccagcact acgacgtact gagcacggaa gttggccagt
     1561 acgtccagcc accagaccca cctcccagcg aacgacgcga ggctggccac ggctatcacc
     1621 gccgccggga tgatgacgag gatcacggcg ggttcagccg gcgtccgcgc cgatcctgga
     1681 acgaatcgcc gtcacctgcc gatgaacgga ggcagcggtt cccgaaccag gagaccgttc
     1741 accggtcacc gacttctcaa catcgaccac cgcgaggtcg gcctcctcga agctgtcatg
     1801 ggccgtcttc agatcatcga aggttgcatc cgtcaggctc cgcccggcgt tttcgagcgt
     1861 gatgatcagc atcccgaccg tcttatgagc ctcgcggaac gggatgcccc gccgaaccag
     1921 ggcctcggcg aggtcgaggc tggcagtcgc tccctccggc atcggaggag cgaactcgat
     1981 gtggccgagc atttcggtca tcgccaacag agacgattcg aggacatcat cagcgtcgaa
     2041 caccagtctc ttgtcctcct gaagatcacg gttgtaggcg aggggaaggc ccttctgtag
     2101 ggcgatcacc gccgccagat cgccggcggc ccgggcagat cgcccccgaa ccagttcggc
     2161 gatatccgga tttcgcttat ggggaagggc ggacgacccg gtgctcaccg aatcgtcgag
     2221 ccgggcccac ccaaactcgg tcgaggtcca cagcacgatt tcctcggcga gacgggagag
     2281 gtcgaccatc gcttgggcac agcagaagac atattccgcg acgtgatctc tggagcccac
     2341 cgcatcgagt gaatttgaga agacagtgcc aaaccccagc aggctggcgc tttgctccgg
     2401 atcgatgcct aaagacgtcc ccgacgaggc tcccgcgccc agcggcgaga catcgagcct
     2461 gcttcggctc ccggtaaacc gatcgacgct cctcagagcc atccaggcgt aagcgagaag
     2521 atggtggccc agggtgctcg gttgtgcctg ttggaggtgg gtataggagg gaaccgggac
     2581 cgcggcggcg gtctcgccga gatcacaaag tcgcaaggca aactcatgga gctgttcgat
     2641 ccgctgtgtc gccgatcgcc ggagatacag cctcaggtcg agcgctatct ggtcgttgcg
     2701 ggaacgggca gtgtgcagct tgccggccac ggcgccgatc aactccccca atcgacgctc
     2761 gactgcgctg tgaacgtcct cgtcggattc ggaaaagacg aactttcctt gctccgcttc
     2821 ggcccggatc gtctccagtc cggcaacgat ttccttggaa tccacagctg aaatgacccc
     2881 tgtggtgccg agcatggtga catgggcaat cgatccgtcc agatcatcga gcagcaaccg
     2941 gcggtcgctc gggtcagtgg tgtagtccca gaggatgctc cctggagcac tctcgaaacg
     3001 cccttcccag agggtcatcc gccgctccca tccccgaaac cacgaattct gagggcgttg
     3061 aggttgatga atccctcggc gtcggcctgg cgatagacat cgtcggcctc gaaggtcgcc
     3121 gagccggcgt cgtacaggct cttgggcgcc ctcctcccat tcaccgtggc agagcccttg
     3181 tagaggacga cccgcgcctc cccagtgacc ggcttttgga tctcatccat gaagctctgg
     3241 agcccgatcc gttccgggct gaaccaaaac ccgttgtaga ccatgtccgc gtatctgggc
     3301 accagctggt cccggagatg catcaattca cggtcgagag tgatcgactc cacggcgcga
     3361 tgcgagaggt gaaggagcgt gccccccggc gtctcgtaca cgccacggct cttcatgccg
     3421 acgaatcggt tctccacgat gtcgatcctc cccaccgcat tgcggccggc gatctcgttt
     3481 gccctctcca gcaagtccac cgggccaaga gactcgccgt tgatcgaaac cggatttccg
     3541 gtctcgaatc caacaaccac ttcctccgcc gcatcggggg cgtcggcggg gtcggtcgtg
     3601 agccggaaca tcttgtcagg tggtgcggtc cacggatcct cgaggacacc gccctcgtac
     3661 gagacatgga gaaggttggc atccgtcgag tagggggctt ccttggtcac cggtaccgcg
     3721 att
//
LOCUS       DBNHXU010000162         6355 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3027211, whole genome shotgun sequence.
ACCESSION   DBNHXU010000162 DBNHXU010000000
VERSION     DBNHXU010000162.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6355)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6355)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6355
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3027211"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1241)
                     /locus_tag="AC161X_04190"
     CDS             complement(<1..1241)
                     /locus_tag="AC161X_04190"
                     /EC_number="6.2.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012711.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015645 - fatty acid ligase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase/ligase"
                     /protein_id="HIR4988780.1"
                     /translation="MAHATGGSGTVIGCFLDRAEQRATQPIWYHQTGGRWLPITWSSA
                     RAAVLELAAGLATLGIGPGDRVGLAGANRPHWVIADYAIQHIGAVVVPIYATSPPDQI
                     AQLLLRTHARVCVVDGDDFLGKLREASVDGLEHVVALHLGDVEHDRLIGHDTLVRRGE
                     DWLEENPTGLSDRLAAIGPDDVATVIFTSGTGGDPKGSILTHGNLLWAADACARAVEV
                     RTREITLSYLPLAHAFERVVTTVIPLVATSERFTCWFVEEIAKLPSALRSVRPTIFVA
                     VPLVWARMEARIQAEIGTTGFLRRRMSRAMLTAGGSAVRKRDEQESVSPPARVGAGTA
                     GLMGRRILKKVGLGRCWYAVSGAAPLQPETQQFFQALGLGLHQGWGLTETAALCAVQR
                     PDDLEIGMVGAPLDGVEIKLG"
     gene            1368..1676
                     /locus_tag="AC161X_04195"
     CDS             1368..1676
                     /locus_tag="AC161X_04195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988781.1"
                     /translation="MCCLFTVLVVFGPRLGIIVWGLASPARWEAAFDTFVWPLLGFVF
                     LPWTTLMYVSVAPLGVTGFDWFWMVLAVFADIASYTGGAYGNKDTIRSYTTPTPPATA
                     "
     gene            complement(1696..2703)
                     /locus_tag="AC161X_04200"
     CDS             complement(1696..2703)
                     /locus_tag="AC161X_04200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009099221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase-like protein"
                     /protein_id="HIR4988782.1"
                     /translation="MSVDLRTTYLGMRLAHPVVPSASPITGDIDHLHQLVEAGASAVI
                     LPSLFEEQVEHEAMAVHMGLEWAKDSFAEATAGYLPELDDYNTGPERYLHLIRTAKAE
                     LAVPVIASLNGTTTGGWTLYAKILADEGIDALELNIYLIAADPAVTGSEVEHRYLSLV
                     EGIRRSVEVPLAVKVGPFFSSMANMATRLVDAGADGLVLFNRFYQPDIDLDRMEVVPN
                     LVLSTPAELRLVLRWIAILRGRITGSLAATTGVDGPIEAAKLVLAGADVTMMASSLLR
                     NGPDRLRETVGGLAVWLEENGYRSLEEAKGSFSQSAVADPAQFERANYMRTLLSYSPQ
                     W"
     gene            complement(2700..6275)
                     /gene="nifJ"
                     /locus_tag="AC161X_04205"
     CDS             complement(2700..6275)
                     /gene="nifJ"
                     /locus_tag="AC161X_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008673498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HIR4988783.1"
                     /translation="MASHFTTVDGNEAAARVAHALSEVIAIYPITPASPMGEHADAWS
                     HLGRPNLWGVVPDVVEMQSEAGAAGAVHGSLQVGALTTTFTASQGLLLMLPNMYKIAG
                     ELTSTVFHIAARTLATHALSIFGDHSDVMAARQTGFAMLASASVQEAQDLAVVAHAAT
                     LKARIPFLHFFDGFRTSHEVSKISAVGDDVLRAMIDEDLIAQHRLRALSPRRPMLRGS
                     AQNPDVFFQAREAINPFYSAVPSTVQAEMDHFADLTGRSYHLFDYVGHPDAERVLVLM
                     GSGAGAASEAVRRLVSQGDRVGLVTVRLYRPFDSESFRAALPDSARAVAVLDRTKEPG
                     SVGEPLYLDVIGALYEGDSPMPVVIGGRYGLSSKEFTPVMASAVFTELERETPKSHFT
                     VGITDDVTHTSLDCSSDAWIEPEDMVRAVFYGLGSDGTVGANKASVKIIGDHTELFSQ
                     GYFVYDSKKSGSRTISHLRFGPQPIASTYLIGSGQATFVACHDFHLLQDVDVLEAAAD
                     GATFLINSPYGPDRVWEHLPRWTQRGIVEKGLDFWVVNATEIAANAGLGGRINTVLQT
                     CFFALSEVMPHDQSMAAITDEVTKAYGKRGEVVVRRNLDAINSALGGLQRVDVPTTFE
                     GGVSSPADIYASRLAAAPDFVQRVTGMMLAGKGDLLPVSAMPVDGTFPTGTAAWEKRS
                     IGNEIPIWDPSICIDCARCALVCPHAAIRFKVFDPAALDNAPDGFVSKEASGTDLAGM
                     ILTVQVAPDDCTGCGVCVDVCPARSKTEVGHRSIDMVPKEAHMERERVNFDFFLTIPD
                     PPRNEVRLDTVKGSQMIEPLFEFSGACAGCGETPYLKLLTQQLGDRLVIANATGCSSI
                     YGGNLPTTPWATDETGRGPAWANSLFEDNAEFGLGMRLGLEAMEADARLLVTALLPEL
                     GDLGVEILGADQTDETGIAAQRRRVEILREQLASVDGLLARRLEGVADALVRTSVWIV
                     GGDGWAYDIGFGGLDHVLASGRDINVLVLDTEVYSNTGGQASKATPRAAVAKFASGGK
                     ATAKKDLGQIAMAYGNVYVAQVAMGANMTQTVKAFAEAEAHHGVSMILAYSPCIAHGI
                     EMSTQMSHQKEAATSGYWPLYRYDPAFEAAGQPGLRLDSRKPTIRFKDFAEKEGRFAM
                     LARANPDRSAELMASAQKDIDDRWQLYEQLVEVHRTAEFTELDDR"
BASE COUNT         1218 a         1970 c         1959 g         1208 t
ORIGIN      
        1 atcccaagtt tgatttcgac cccgtccaga ggagctccca ccatgccgat ctcgaggtcg
       61 tcaggtcgct ggacggcaca cagtgctgcc gtctcggtga gaccccaacc ctgatggagt
      121 ccaagaccaa gggcttggaa gaattgctgt gtctctggct gaagaggcgc ggctcctgag
      181 accgcatacc agcaccttcc caaaccaacc ttcttcagaa tccgccttcc catgagcccg
      241 gcagtgccag cgcctacacg tgctggtgga gatactgatt cctgctcatc gcgctttcgg
      301 acggccgatc cccctgcagt caacatcgct cgcgacatgc gccgacgcag aaaccctgtt
      361 gtcccaatct ctgcctggat ccgcgcctcc atcctggccc agaccaatgg cacggcgaca
      421 aatatcgtgg gccggaccga gcgaagtgcg gaggggagtt tggcgatctc ctccacgaac
      481 cagcaggtga accgttcact ggttgctacc aggggaatca cggtggtgac gacgcgttcg
      541 aaggcgtgag cgagggggag ataggagagt gtgatctcac gggtgcggac ctcaactgct
      601 cgagcgcagg cgtccgccgc ccagaggaga ttgccgtggg tgaggatgga gcctttgggg
      661 tcacccccgg taccggaggt aaatatgacc gtggcgacat cgtcagggcc gatagccgca
      721 agacgatctg aaaggccggt cggattctcc tcgagccaat cctccccacg acgaacaagg
      781 gtgtcatgtc cgatcagccg gtcatgttcc acatcgccga gatggagcgc caccacgtgt
      841 tccaatccgt caaccgaggc ttcccggagc ttccccaaga agtcgtcccc atccaccaca
      901 caaaccctgg cgtgggttcg caatagcaac tgggcaatct ggtcgggcgg actcgtcgcg
      961 tagatcggca cgaccactgc cccgatatgt tgaattgcgt agtcggcgat cacccaatgg
     1021 ggtcgatttg ctcccgccag gcccacccgg tcaccgggac cgatccccag tgtggccaac
     1081 cctgccgcca gctcgagtac cgcagcccgt gctgagctcc aggtaatcgg gagccaccta
     1141 ccgccggtct ggtggtacca gattggttga gtggctcgct gctccgctcg atcgaggaag
     1201 caaccgatca cagtccctga cccgccggtt gcatgcgcca tggcggccga ggctacgccg
     1261 aacatcgatg accggcccgg accgagattt gccttcaggc tcgcatcggc cgacacgtgg
     1321 tgactaggtt cccacaagcg gaatccgatt agggaggact gaaggtcatg tgctgtttgt
     1381 tcaccgttct agtggtcttt ggacctcgcc tcggcatcat cgtctggggc ttggcctcac
     1441 cggcccgctg ggaagcggcc tttgatacat ttgtctggcc cctcctcggt ttcgtgtttc
     1501 tcccgtggac gacactcatg tacgtctcgg tcgctccgct tggagtcact ggcttcgatt
     1561 ggttctggat ggttctcgca gtcttcgccg acatcgcctc ctacacgggt ggcgcttatg
     1621 ggaacaagga cacgatccgg agttacacga ctccgacccc gccagctact gcgtaaccga
     1681 cgcgacgcca aagagtcacc attgaggtga gtaggacagc aacgtgcgca tgtaattggc
     1741 cctctcgaac tgggccgggt cggcgaccgc ggactgactg aaggatccct tggcttcctc
     1801 gagtgatcga tagccgttct cttcgagcca gactgccaga ccacctacgg tctcgcggag
     1861 ccgatccggc ccattccgga gcagagacga cgccatcatc gtgacgtccg ccccggccag
     1921 gaccagcttg gccgcctcaa tgggaccgtc gacaccggtc gtcgccgcca acgacccggt
     1981 gatcctcccg cggaggatgg caatccaacg gagcactagg cgaagttccg ctggtgttga
     2041 caggacgagg ttgggaacca cttccatgcg atcgagatcg atgtccggct ggtagaagcg
     2101 attgaagagg accaggccat cggccccggc atccacgagt cgggtcgcca tgttcgccat
     2161 cgacgagaag aacggcccga ctttgactgc gagcggcacc tccacggatc ggcggatccc
     2221 ttcgaccagg ctgaggtatc ggtgttccac ctcgctcccg gtcacagccg ggtccgcggc
     2281 aatgagatag atattcagtt cgagagcgtc gattccctca tcggccagaa tcttggcgta
     2341 gagggtccag cccccggtcg tggtcccgtt gagactggcg atgactggca cggccagctc
     2401 ggccttcgcc gtccggatca agtggagata tcgctccggg ccagtgttgt agtcatccaa
     2461 ctccggcagg tagccggcgg tggcctcggc gaaggagtcc ttcgcccatt ccaagcccat
     2521 gtgcacggcc atcgcctcgt gctcgacctg ctcctcgaat agcgaaggca ggatcacagc
     2581 cgatgcgccc gcttcgacta gctggtgaag atggtcgatg tcaccagtga tgggggaagc
     2641 tgaaggcacc accggatgag ccagcctcat tcccaagtag gtggtgcgca ggtcgacgct
     2701 catcggtcat ccaactcggt gaactcagcg gtgcgatgaa cctcgaccag ctgttcgtag
     2761 agttgccagc gatcgtcgat gtccttttgg gcgcttgcca tgagctcggc cgaccgatcg
     2821 gggttggctc gggccagcat tgcgaaccgg ccctccttct cggcaaaatc cttgaaacga
     2881 attgtcggct tgcggctgtc gagtcgtagc cctggctgtc cggcagcctc aaaggcgggg
     2941 tcatatcgat acaagggcca gtagccggag gttgcagcct ccttttggtg actcatctgg
     3001 gtagacatct cgatcccgtg agcgatgcac gggctgtaag cgaggatcat ggaaacgccg
     3061 tgatgcgcct ctgcctcagc gaacgctttc acggtctggg tcatgttggc gcccatcgcc
     3121 acctgggcca cgtagacatt cccgtaggcc atcgcgatct gacccaggtc cttcttcgcc
     3181 gtggccttcc cgcctgaggc aaacttcgcc acggccgcac gtggtgtggc cttggaggcc
     3241 tgcccaccgg tgttggaata gacctcggtg tcgaggacca ggacgttgat gtccctgccc
     3301 gaggcgagaa cgtggtcgag acccccgaaa ccgatgtcgt aggcccaacc gtcgccgccg
     3361 actatccaga cggaggtgcg aaccaaagcg tccgccaccc cctcgagtcg gcgggcaagg
     3421 agcccatcca cggacgccaa ctgctctcga aggatctcaa cccgacgtct ctgagcggca
     3481 atcccagttt cgtcggtttg gtcggctccc aggatctcca cccctaagtc tccgagttcg
     3541 gggagaagcg cggttaccag aaggcgggca tcggcctcca tggcttcgag gccgaggcgc
     3601 atccccagcc cgaactcggc attgtcctcg aatagtgagt tggcccacgc cggcccgcgc
     3661 ccggtttcat ctgtcgccca cggcgtggtg ggaaggttgc ctccatatat cgacgagcat
     3721 ccggtggcgt tcgcaatgac caggcggtca cccagttgct gggtgaggag tttgaggtag
     3781 ggcgtctctc cgcacccagc gcaagctccg gagaattcga acaacggctc gatcatctga
     3841 gaccccttga ccgtgtcgag ccttacctca ttacgcggag ggtcggggat tgtcaggaag
     3901 aagtcgaagt tgacccgctc ccgctccatg tgggcttcct tggggaccat gtcgatcgag
     3961 cgatgcccca cctcggtctt ggatcgcgct gggcaaacgt cgacgcatac tccgcaacct
     4021 gtgcagtcgt cgggagcaac ctgcactgtg aggatcatgc ccgcaaggtc ggtcccgctg
     4081 gcctccttgg atacgaaccc atcgggggca ttgtccagtg cagccggatc gaagactttg
     4141 aatctgatgg cggcgtgggg gcagaccaaa gcacaccggg cacagtcgat gcagattgag
     4201 ggatcccaga tcgggatctc gtttccgatc gaacgcttct cccaagcggc ggtcccggta
     4261 gggaatgtgc cgtcgacggg catcgccgag accggcagca gatctccctt ccctgccagc
     4321 atcatcccgg tcacccgctg aacgaagtcc ggggcggctg cgagtcgaga ggcgtagatg
     4381 tctgctgggc tgctcactcc tccttcgaag gtggtgggta cgtccacccg ttgcagacca
     4441 ccaagcgccg agttgatggc atcgaggttg cggcgtacga cgacctcgcc tcgcttgccg
     4501 taggccttgg tcacctcatc ggtgatggcg gccattgact gatcgtgtgg catgacttcg
     4561 gagagggcaa agaagcaggt ttggagcacc gtgttgatcc gacctccgag cccagcgttg
     4621 gcggcgattt ctgtggcgtt gaccacccag aagtcgaggc ccttctcgac gattccgcgc
     4681 tgagtccaac gagggaggtg ttcccacact cggtcgggcc cgtacgggct gttgatgagg
     4741 aaggtggccc catctgccgc cgcttccaat acgtccacat cttgaagcaa gtggaagtca
     4801 tgacaggcca cgaatgtggc ttgcccggac ccgatcagat aggtcgatgc aatcggctgt
     4861 gggccgaatc ggaggtggga aatcgttcgt gacccggact tcttcgagtc gtagacgaag
     4921 tagccctggg agaagagctc ggtgtggtca ccgatgatct tgactgaagc cttgttggct
     4981 ccgactgtgc cgtcactgcc cagtccgtag aagacggcac gcaccatgtc ttcgggctca
     5041 atccaggcat ccgagctgca atccaggctc gtgtgggtta catcgtcggt gatccccacc
     5101 gtgaaatggg acttcggagt ctccctctcc agttcggtga aaacggctga tgccatcact
     5161 ggggtgaact ccttcgaaga cagtccatac cggccgccaa tgacgactgg catggggcta
     5221 tctccctcgt agagcgcgcc gatcacgtcc aaatagagag gctcgccgac cgatccaggc
     5281 tctttggtcc tgtcgagaac ggcgacggcg cgagccgaat caggcagtgc cgctcggaag
     5341 gattcgctgt cgaacggtcg gtagagacga acggtcacca gaccgacgcg atctccctgt
     5401 gacaccagac gccgcaccgc ttcggaggca gcgcccgctc cggatcccat caggaccagg
     5461 accctctccg cgtcgggatg ccccacgtaa tcgaagaggt ggtagctgcg gccggtgagg
     5521 tcggcaaagt gatccatttc tgcctggacc gtcgatggaa ccgccgagta gaacgggttg
     5581 atcgcctccc tcgcctgaaa gaagacatcc ggattctgag ccgagccgcg gagcatgggg
     5641 cgtcggggac tcagagcacg tagccgatgc tgggcaatca gatcctcatc gatcatggcc
     5701 cgaagaacgt cgtctccaac tgcggagatc tttgacacct cgtgcgaagt gcggaaccca
     5761 tcgaagaagt ggaggaaggg gattcgggcc ttcaacgtgg ccgcgtgagc caccactgcg
     5821 aggtcctgtg cttcctgcac tgatgccgag gcgagcatgg caaacccggt ttggcgggct
     5881 gccatcacat cggagtggtc accgaatata gagagggcat gggtggcgag ggttctggct
     5941 gcgatgtgga agaccgtcga cgtcagctcc cctgcaatct tgtacatgtt gggcagcatc
     6001 agcagcaggc cctgagaagc agtgaaggtc gttgtcaacg cgcctacctg aagcgatccg
     6061 tgcacggcgc cggcagcgcc cgcctcactt tgcatctcca ccacatcggg gacgacaccc
     6121 caaagattgg ggcgaccgag gtgcgaccag gcgtctgcgt gctcacccat cggcgaagcc
     6181 ggagtgatcg ggtagatggc aatcacctcg ctcagggcat gcgccactcg ggcggcggcc
     6241 tcgttgccgt cgaccgttgt gaagtgagag gccatgggtt caaaggtaag gagacgacat
     6301 cctgcaaagg agtgccgaag gtcacgacac ccgcggtcga agcgccctag tgcct
//
LOCUS       DBNHXU010000163         1517 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1242951, whole genome shotgun sequence.
ACCESSION   DBNHXU010000163 DBNHXU010000000
VERSION     DBNHXU010000163.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1517)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1517
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1242951"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..869)
                     /locus_tag="AC161X_04210"
     CDS             complement(<1..869)
                     /locus_tag="AC161X_04210"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988784.1"
                     /translation="MPRVRWGWVLVAAIPTFFIGYFFVYPLVRILSLGLSELDVGAIS
                     VEARLLKVGWFTLWQAGLSTILTFVVAAPMTWAVSNFEFRGRRLATALVTVPFVLPTV
                     VVGSAFVALGWRDSIFAILAAHVFFNAAVVVRTVGTLWSRLDPDLHDAARVLGAGEWR
                     TFREVTLPLLRPAIAAAASIVFLFTFTSFGVVLILGGYQFATLEVEIYRQAVDLFDLP
                     LAAALAVVQLVGVSGALYAYSRFQERHTASWALRSESDRMRPVGNTRWMVFASVAATL
                     TAMAVPLVVLVGRS"
     gene            complement(869..>1517)
                     /locus_tag="AC161X_04215"
     CDS             complement(869..>1517)
                     /locus_tag="AC161X_04215"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR4988785.1"
                     /translation="DVCINYDKQWFADNGLTVPTTLDQLRDPTYASLLTVEHPATSSP
                     GLAFMLATIDQYELHGWLDFWTDLRDGGVNVAPDWNTAYYGDFTRYGGDSPMVLSYAS
                     SPPAEVVFAEEPIDEAPTGVIEDGCYRQIEYAGVLNGTLYPDASGHLIDYMLSVEFQE
                     KIPLTWFVFPANRDAELPEVFTENTTVPEAPVRFTYESFADREVWIDEWVEVMEG"
BASE COUNT          322 a          474 c          494 g          227 t
ORIGIN      
        1 gaccgcccca ccaggactac tagcggcacc gccatcgcgg tcaaagtggc ggctactgat
       61 gcgaacacca tccacctggt gttaccaacc gggcgcatcc ggtcgctctc cgagcgcaat
      121 gcccacgagg cggtgtgccg ctcctggaag cgcgagtagg cgtagagggc gccactcact
      181 ccaaccaact gcactacggc gagagcggcg gccagaggta ggtcgaagag atcgactgcc
      241 tggcgataga tctcgacctc gagcgtggcg aactgatacc cgccgaggat cagcacaact
      301 ccgaaggaag tgaaggtgaa cagaaagacg atcgacgccg ccgctgcgat ggcggggcga
      361 agcaatggca gagtgacctc tcggaaggtc ctccattcgc cagccccgag aacccgggcc
      421 gcgtcgtgca ggtcggggtc gaggcggctc cacagggtgc ccacggttcg caccaccact
      481 gccgcgttga agaagacgtg tgcggcgagg atggcgaaga tcgagtcgcg ccaccccagc
      541 gctacgaagg cggacccgac caccacggtg gggaggacga acggaaccgt gaccagcgcc
      601 gtggccaggc gccttccccg gaactcgaag ttggagacgg cccaggtcat cggagccgcg
      661 accacgaagg tcaggatcgt ggacagcccc gcctgccaca gcgtgaacca gccgaccttg
      721 agcagccgcg cctcgacgga gattgccccc acgtcgagct cggagaggcc aagactgagg
      781 atccgcacca gcggatagac gaagaagtag ccgatgaaga acgtgggaat cgcggccacc
      841 agcacccatc cccatctgac gcggggcatc agccctccat cacctcgacc cattcgtcga
      901 tccagacctc acggtcggcg aagctctcgt aggtgaaccg gaccggtgcc tccgggacag
      961 tcgtgttttc ggtaaagacc tcgggaagtt ccgcatctcg attggcgggg aagacgaacc
     1021 aggtgagcgg gatcttctcc tggaactcga cagacagcat gtagtcgatc agatgcccgg
     1081 aggcatcggg gtacaaggtc ccgttgagaa ctccggcgta ttcgatctgg cgatagcacc
     1141 cgtcctcgat cactccggtt ggcgcctcgt cgatcggctc ctcggcaaac accacttcgg
     1201 cgggcggtga cgaggcgtag gacagcacca tcggcgaatc cccgccgtag cgggtgaaat
     1261 cgccgtagta ggcggtgttc caatcagggg ccacgttgac tccgccgtcg cggaggtccg
     1321 tccagaagtc gagccagccg tgcaactcat actggtcaat ggtggccagc atgaaagcca
     1381 aacccggcga agaggttgcc gggtgctcga ccgtgagcaa tgaggcgtag gtgggatcgc
     1441 gcagttggtc caaggtggtg ggcaccgtga gcccgttgtc ggcgaaccat tgcttgtcat
     1501 agttgatgca gacgtcc
//
LOCUS       DBNHXU010000164         4618 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4219060, whole genome shotgun sequence.
ACCESSION   DBNHXU010000164 DBNHXU010000000
VERSION     DBNHXU010000164.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4618)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4618
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4219060"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(104..829)
                     /locus_tag="AC161X_04220"
     CDS             complement(104..829)
                     /locus_tag="AC161X_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012795737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988786.1"
                     /translation="MNTRIFRTEVRDELLGIVREPTALLFSVLVPVAFFALFAAMFGN
                     EGDGFGVGSLATYGAFAVLSAVLINPGVGLADARERGWLRVKRADGTPLGVTLSAKVT
                     AALPYAIVTLTGITLVARFTAGPLDVPQVLRVIGVLLLGALPFSLFSLAVGARFSLNS
                     AIAVLNAMLIPSVILSGLWFPLEMLPTWLGEIAVYLPPYHLAQLALAQVEGGGAWDHV
                     AYLLGTTIVGAIAAAVAYRSAEV"
     gene            complement(826..1722)
                     /locus_tag="AC161X_04225"
     CDS             complement(826..1722)
                     /locus_tag="AC161X_04225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020665950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988787.1"
                     /translation="MEHVLEVRGLVHRYGDHTALAGVDLTVKSGECVALLGPNGAGKT
                     TLVRNVIGLIRGNADLIEVAGGDPRRAETRRRLGVVQQAVGFPRSLTVGEIVGGAAAR
                     RGCPPEAASNAIAEMGLAGLEKRRAVKLSGGQQQRLQLAMGLVADPVLLVLDEPTEGL
                     DVEARRRFWKTIRRRLDDGAGVLITTHLVEEAAAVADRVVVVDRGRIVAEGTPDELRR
                     TLPDRRIEVRTKVPVSVIRALDSVHSVELRDGRTVIAATDSEQVVSTLLDLDPEANDL
                     TVATASLEEVLVALTNHEEVAA"
     gene            complement(1965..2810)
                     /locus_tag="AC161X_04230"
     CDS             complement(1965..2810)
                     /locus_tag="AC161X_04230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988788.1"
                     /translation="MSVDLDRQLREYCRQMDEQQGAFSFQDILDRAEGMPVIPRPMSN
                     RPTPRRAWIAAATAFVVFIILIGVWFLPPTDQTPGPVDQPTTTVAPARSFPETDLEAG
                     TYRIEPSESSVAGVTLTLPAGWEAPYGLPYAVKHSEQDGELAFYFVTVDRIFSDPCVG
                     VEGGEIGPSVDDLVTALAEQPLTETSEPIATTLGGLPATRIDLTVPAGFDTSECNAVY
                     GLQIWYSRPADNYFVLLEDGTASVYVLDVNGERQVFLTQYRAATSAEDIAEMQTVIDS
                     IVIDQ"
     gene            complement(2807..3274)
                     /locus_tag="AC161X_04235"
     CDS             complement(2807..3274)
                     /locus_tag="AC161X_04235"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4988789.1"
                     /translation="MLPDQTRVDGFVEFVETNESRLRHALTAALGFDAGKEAAAEAFA
                     YGWEHWERVGAMANPVGYLYTVGRSNGRRLLSTARPVFPSIPEDRTPWFEPGLPAALA
                     ALPERERTVVLLLHGYGWHMTEVAETLGVSKSTVQTHAERGMRKLRRKLGVER"
     gene            3421..4203
                     /locus_tag="AC161X_04240"
     CDS             3421..4203
                     /locus_tag="AC161X_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988790.1"
                     /translation="MMQRKLRHIGLVTAVLGLAACSIQGGEDTSTTASPTPSSTSAPA
                     SSTSAPAVATTTATSSPEWPSSGPVEPGTYHHPPSAWNVAGFTMTLPEGWEIDPAIPP
                     GVVKNYDSEEEVSFYPVIVDAIYSDPCVGTAGQEGGELMEVGPSVEDLARALLDQPHT
                     VATEPVDTTLGGLPAKRVDLTVEDDPETATCNIDVPGHLQIWHSVPVDKFFVLLGDGT
                     ASVYIVDVNGERQVFLTQHFNGTPAADVSQMQTIIDSIRIDT"
     gene            complement(4292..4365)
                     /locus_tag="AC161X_04245"
     tRNA            complement(4292..4365)
                     /locus_tag="AC161X_04245"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(4329..4331),aa:Ala,seq:cgc)
BASE COUNT          903 a         1458 c         1414 g          843 t
ORIGIN      
        1 ccgacgagga tgaatgcgaa gaaaaccagt ggggcccaac gcgagaaacc ggaacgcctc
       61 gtgtcgaggc gttccgagtt cccgagtctg agtgtcgtgt cgttcacact tccgcggaac
      121 ggtacgcgac cgctgctgcg atcgctccca cgatcgttgt tcccaggaga taggcgacgt
      181 gatcccacgc ccctccgcct tcgacctgcg ccagcgccag ttgtgccagg tgataaggcg
      241 ggaggtacac cgcgatttct ccgagccacg tcgggagcat ctccagcgga aaccagaggc
      301 ccgagagaat caccgaagga atcagcatcg cgttgagcac ggctattgcg ctgttgagac
      361 tgaacctcgc cccgacagcc agagagaaga ggctgaatgg caaggctccg agtagcagga
      421 caccgatgac acgtaggacc tgaggcacgt ccagcggtcc agcggtgaaa cgggcgacca
      481 gggttatccc tgtcagggtg acgatggcat aaggcagtgc tgccgtgacc ttcgcggaca
      541 gtgtcacgcc aagcggcgtt ccgtccgccc gcttgacgcg gagccagcct cgctccctgg
      601 cgtcggcgag gccgacaccc gggtttatca gaaccgctga gagcactgca aacgccccgt
      661 acgtggccaa tgaacccacg ccgaagccgt ctccctcgtt gccaaacatc gctgcgaaca
      721 gtgcaaagaa tgccactggc accagcaccg agaaaaggag agcggtcggc tccctgacga
      781 ttcccaggag ctcgtctcgc acctctgtgc gaaagatccg ggtgttcatg ctgctacctc
      841 ctcatggttg gtcagtgcga cgaggacctc ctcgaggctc gccgtcgcca cggtcaaatc
      901 gtttgcctcg gggtcgaggt cgagcagcgt gctgacgacc tgctcactgt cggtcgctgc
      961 aatcacggtg cgcccgtcac gaagctcgac cgagtgcacc gagtcgagcg ctctgatcac
     1021 actcaccggt accttcgtcc ggacttcgat ccgacggtcg gggagcgtcc gacgcagctc
     1081 gtctggcgtc ccctccgcga ctattcgtcc gcgatcgacc acgacgaccc gatcggcgac
     1141 cgctgccgcc tcttcaacga ggtgcgtcgt gatgagcaca ccggctccgt cgtcgagacg
     1201 tcgccggatc gttttccaga accgacgccg tgcctccaca tcgagtcctt cggtgggctc
     1261 gtccagtacc agcagaaccg gatcggcaac aagccccatc gcgagctgca accgctgttg
     1321 ctgtccgccg gagagcttga ccgcccgccg cttctccaag ccggccaggc ccatctcggc
     1381 gatggcattg ctggctgcct ccggagggca accccggcgg gcggccgcac ccccgacgat
     1441 ctcgccgacg gtgagtgacc gcgggaaacc gaccgcttgt tggacgacac cgagcctgcg
     1501 gcgggtctcg gcgcgacgag ggtcgccacc tgccacttcg atgaggtcgg cgttgcctct
     1561 gatcaatccg atcacgttgc ggacgagggt cgtcttaccg gccccatttg ggccgagaag
     1621 tgcgacacac tcccccgact tgacagtcag atcgactccg gcaagcgccg tgtggtcacc
     1681 gtatcggtga acgagacctc gcacttcgag cacatgttcc atctactacc tccttgacac
     1741 cgatcatgcg gaatgaccgg tgcgagcggt agtgagatcg atcacgaact caacgtgaca
     1801 gatgtcatcc tgatcaagcc aggcctaggc gcccgtcctg tggagcgtca atgcagctag
     1861 cggaccctga ccggggtgga attgtggagc tgacgggatt tgaacccgtg acctccttgt
     1921 gggacgaccg acgtgagaca gcgtgagatg tcgcgaaagg tggcctactg gtcgatcacg
     1981 atggagtcga tgacggtttg catttcagca atgtcctcgg cgctagtcgc ggctcgatat
     2041 tgcgtgagga acacctggcg ttctccgttc acgtcgagga catagacact ggccgtaccg
     2101 tcttccagaa ggacgaagta gttgtcggcc ggccggctgt accagatttg aagaccgtag
     2161 acggcgttgc attctgaggt gtcgaagccg gctgggaccg tcaggtcgat tcgcgtagcc
     2221 ggtaggcctc ccagcgtcgt ggcgatcggc tcactcgtct ccgtgagagg ctgttcggct
     2281 aaggccgtga ccagatcatc gacgctggga ccgatttccc ctccttcgac tccaacacac
     2341 gggtcggaga agatccggtc gacggtcacg aagtagaagg ccagctcgcc gtcttgttcg
     2401 gagtgtttga cggcatacgg aaggccgtac ggggcctccc agccggcagg cagggtcagc
     2461 gtcacaccag cgacggacga ctccgaaggc tcgatgcgat atgtgccggc ctcgaggtcg
     2521 gtctccggaa aggatctggc cggtgcaacc gtcgtggttg gttggtccac aggacccggg
     2581 gtttggtccg tgggggggag gaaccagacg ccgatgagaa taatgaagac gacgaaggcc
     2641 gtagcagcgg cgatccacgc acggcggggt gttggtcggt tgctcattgg tcttgggatc
     2701 accggcatcc cttctgcccg gtcgaggatg tcctggaacg agaaagcacc ctgttgctcg
     2761 tccatttgac ggcagtactc gcgcagttgc cggtctagat ccacactcat cgctcgactc
     2821 ctagtttccg ccggagtttc cgcattccgc gctccgcgtg ggtctggacc gtgctcttcg
     2881 acactccgag agtctccgcc acttcagtca tatgccatcc gtatccgtgg agcaacaaga
     2941 ccaccgttcg ctccctttct ggcaaagcgg cgagagcggc aggcaggcct ggttcgaacc
     3001 agggtgtccg atcctcggga atcgacggaa acacaggtct tgctgtggag agcagtcttc
     3061 gtccgttgga ccggccgacg gtgtacaggt atccgaccgg gttggccatg gcccccaccc
     3121 tttcccaatg ctcccatcca taggcgaaag cttccgctgc ggcctccttc ccggcatcga
     3181 aacccaatgc tgcagttagg gcgtgacgca gtctgctttc gttcgtctcc acaaactcga
     3241 cgaaaccgtc gacccgggtc tggtcgggta gcaacctgtc tcctgagcta acacttgata
     3301 aagctcagca acccgtcagc gtacgacaga aaagtcgcga aggtttgtcg aaatgcccga
     3361 cagggtcggg cctcagaaga tccttgtcgt acgagcgtga ccgctcgtgc tttctcaatc
     3421 atgatgcaaa ggaagctgcg acacatcgga ctcgtgaccg ctgtcctggg gctggctgcc
     3481 tgctccatcc aaggcggcga agacacctcc acaaccgcta gtccgacccc gtcgagtacg
     3541 tcggcacctg cgtcgagtac ctcggcacct gcggtcgcaa cgacgactgc gacgtccagc
     3601 cccgagtggc catcaagcgg accggtcgag cccggcacct atcaccatcc gccctccgca
     3661 tggaacgtcg ccggcttcac aatgaccctg cccgaaggct gggaaattga cccggcgatc
     3721 ccgccagggg tcgtcaagaa ttacgactca gaggaggagg tctcgtttta ccccgtcatc
     3781 gtcgatgcga tctactccga tccgtgcgtg ggaacggcgg gacaagaagg gggtgaactg
     3841 atggaggtcg gcccaagtgt cgaggatctc gcccgagccc tcctcgacca gcctcacacg
     3901 gtggctaccg agccagtaga cacaacactc ggcggcctac cggcgaaaag agttgacctg
     3961 accgtggaag acgatcccga aacggcaacg tgcaacatcg acgttcccgg gcatcttcaa
     4021 atctggcaca gcgtgccggt cgacaaattc tttgtgctct tgggtgacgg gaccgccagc
     4081 gtctacatcg tcgatgtgaa tggcgagcgc caggtgttcc tcacccaaca tttcaacggg
     4141 acccccgccg ccgacgtcag ccaaatgcaa accatcatcg actcgatcag gatcgacaca
     4201 tagccaatcg cctgtccagt tccggacacc caagacgcga gactgtccga acattacgcg
     4261 tgtcggataa caacttgacc aggatgttca gtggagctga cgggatttga acccgtgacc
     4321 ccctcgttgc gaacgaggtg ctctgccagg ctgagctaca gccccttgcg ggagacgggg
     4381 attgtaccgg cgggtggttg gtgggccaga gcgccgattc tcggtcgccg ttgcgcagat
     4441 ctggcgcaac gtacctcagg taagccggga gaggtcctcg cggaggcgtt cctggacgac
     4501 agtcgccgtg aggtcggagc gggagcgttc caccagggcc cgggccacgt cggtggtccg
     4561 gcgcagaccg gatgtcatcc cgtcggggta gtccatcgag gccagcagat cgacgatc
//
LOCUS       DBNHXU010000165         2487 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4418326, whole genome shotgun sequence.
ACCESSION   DBNHXU010000165 DBNHXU010000000
VERSION     DBNHXU010000165.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2487)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2487
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4418326"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1732)
                     /locus_tag="AC161X_04250"
     CDS             complement(<1..1732)
                     /locus_tag="AC161X_04250"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR4988791.1"
                     /translation="MPFLLSLLLAIGTLGPQSAAPDESVTVRLLIPEEAGTTLESSGI
                     AATAEIGGHHLGWTEIEVPINGTIEQTVEAISEALGQPVLVEQRYDLLGPTDEPNFAD
                     QWYHHNTGQTNGTTDADIDSPTAWNQALGGGVVVAVIDSGVDLTHPDLSTQIWANSGD
                     PAGGGDGDGNGLVDDFRGWDFVNEDNNPSPVGSGASAAHGTEVAGVIAAASNGVGIVG
                     VAPGSKVMALRACDSTGCWSLNAAEAIYYAADMGADVINLSFGGLGDEDPLIEDAIAH
                     ARSRNVVVVAAAGNERRNLDNLSPGQQFIPAGLPLSNIIAVGASDDRDSLAADFSNYG
                     PETVDLAAPGVDVMSTGIVSIAPYVFVSGTSFSSPIVAGAAALLLSHDPGIGYQELIA
                     RLEGFADHPGGISGLTGHGRLNIGRSVTLRFIDTAGSVFVDPIDWLAERNISQGCNPP
                     FNHRYCPQNPVSRGEMAVFLSRAFHLPTTSTNFFDDDEGRFYEGAANRLAAAGLTVGC
                     GTRRYCGDDDIRRDEMAAMLARATHLPNSSQDFFVDDEGSVFESAINKIAAAGITLGC
                     NPPTNNRYCPR"
     gene            1796..>2487
                     /locus_tag="AC161X_04255"
     CDS             1796..>2487
                     /locus_tag="AC161X_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4988792.1"
                     /translation="MLSLATFALTVALLPTAALAPGGTFIDDDGSVHEGGIEAVAFEG
                     ITVGCNPPFNDRFCPDRLLTRAEMATMIARALSLPASSTNHFVDDNGHILEGAINRLA
                     DAGITQGCNPPSNNRFCPDRNLTRAEAAAFISRAFGLPVSGHDFFIDDNGHVLEGSIN
                     RIAEAGITVGCNPPTNNRFCPNRVLTRGEMATLLTRAMHLTATKPSARPALDWDLVVD
                     GLDTPIQVLNPPG"
BASE COUNT          453 a          792 c          761 g          481 t
ORIGIN      
        1 cccgggggca gtagcggttg ttggtcggtg ggttgcagcc cagtgtgatc ccggccgcgg
       61 cgatcttgtt gatcgcactc tcgaacaccg acccctcgtc atcgacgaag aagtcctggg
      121 atgagttagg gagatgcgtg gcccgagcca gcatcgccgc catctcgtcc cgtctgatgt
      181 cgtcgtcacc gcagtatctc cttgtcccgc agcccaccgt cagaccggct gcggctaacc
      241 gattcgccgc tccttcgtag aaccttccct cgtcgtcgtc gaagaagttg gtcgatgtcg
      301 tcgggaggtg gaaggcccgg gatagaaaca ccgccatctc accacgagag accgggttct
      361 gcgggcaata gcgatgattg aacggagggt tgcagccctg gctgatgttt cgctccgcca
      421 gccagtcgat cgggtccaca aagaccgaac cggcggtgtc aatgaagcgc aaagtcacgc
      481 tgcgaccgat gttgagtcgg ccgtgcccgg tcagcccgga gatccccccg ggatggtcgg
      541 cgaatccttc gagtcgggcg atcaactctt ggtagccaat gccgggatcg tgcgacagaa
      601 ggagggcggc cgctcctgcg acgatggggg atgagaacga cgtgccactc acaaacacat
      661 agggggcgat gctgacgatg ccggtactca tcacgtccac gcccggagcg gcgagatcga
      721 ccgtctcggg gccgtagttg ctgaagtcgg cggccagact gtccctgtcg tcactggctc
      781 cgaccgcgat gatgttggag agcgggagcc ctgccgggat gaactgctgt ccggggctga
      841 ggttgtcgag gtttcgtctc tcgttgccgg cggcggccac cacgaccacg tttctggaac
      901 gagcgtgggc aattgcgtcc tcgatcagtg ggtcctcatc gccaaggcct ccaaacgaga
      961 gattgatcac gtccgccccc atgtcggctg cgtagtagat ggcctcggcg gcattgaggc
     1021 tccaacatcc cgtcgagtcg caggcgcgga gagccatgac tttcgaaccg ggcgccactc
     1081 cgacgatgcc gaccccgtta cttgctgccg cgatgactcc agccacttcc gtgccatgag
     1141 cggcgctcgc gcctgatccc accgggctcg ggttgttgtc ctcgttgaca aaatcccaac
     1201 cccggaagtc atccacgaga ccgttcccat ctccatcacc acctccagcg ggatcgccgg
     1261 agttggccca aatctgggtg ctcagatccg gatgagtcag gtcgacaccg gaatcgatca
     1321 ctgcgacaac aacacccccg cccaatgcct ggttccatgc ggtgggggag tcaatgtcgg
     1381 cgtcggtggt gccattggtc tgaccggtgt tgtggtggta ccactggtcc gcgaagttgg
     1441 gctcgtcggt cggaccaagc aggtcgtacc gctgctccac caacactggt tgccccagcg
     1501 cctcggagat ggcctcgacc gtctgttcga tggttccgtt gatcggcacc tcgatctcgg
     1561 tccatccaag gtgatggcct cctatctcgg cagttgcggc gattccgctc gattccagcg
     1621 ttgtgccggc ctcctccgga atcagcagcc tgaccgtcac gctctcatcc ggggcggcgg
     1681 attgaggtcc caaggtgcca atcgccaata gcagggacag gaggaacggc atcgggtcta
     1741 atcctcccag actttctggc tttgccgggc gaagcggtta ccttggtcga ggtcgatgct
     1801 ttccctcgct accttcgcac tgacggtcgc gttgctcccc acggcggcac ttgccccggg
     1861 aggaaccttc atcgacgacg acggaagcgt ccacgaaggt ggaatcgagg cagtcgcctt
     1921 cgagggaatc acggtcggat gcaatccacc tttcaacgac cggttctgcc cagatcggct
     1981 gctgaccagg gcagaaatgg cgacgatgat tgccagggcc ttgtcgcttc cggcttcgag
     2041 caccaatcat ttcgtcgacg acaacgggca cattctggag ggggcgatca accggctcgc
     2101 cgatgccgga atcacccagg gttgcaaccc tccctcgaac aaccgcttct gcccggaccg
     2161 gaatctgacc cgagccgagg ccgctgcctt catctcgagg gcgtttggat tgccggtatc
     2221 ggggcatgac ttcttcatcg atgacaacgg acacgttctc gaaggctcga tcaaccgaat
     2281 cgccgaggct ggaatcacgg ttggatgcaa cccaccgacc aacaatcggt tctgtcccaa
     2341 ccgtgtactc accagaggcg aaatggccac cctcctgacc agggccatgc acttgaccgc
     2401 cacgaagccg tcggctcggc cggcgttgga ttgggatctg gttgtcgacg ggctcgatac
     2461 cccgatccag gtgctcaacc caccggg
//
LOCUS       DBNHXU010000166         4628 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3823811, whole genome shotgun sequence.
ACCESSION   DBNHXU010000166 DBNHXU010000000
VERSION     DBNHXU010000166.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4628)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4628)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4628
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3823811"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(22..516)
                     /locus_tag="AC161X_04260"
     CDS             complement(22..516)
                     /locus_tag="AC161X_04260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988793.1"
                     /translation="MEGQFFFTTVAGLSVSLAGFAALIAWLRDDSTTWDPINLWRVKT
                     IVRQAFTVAFLALLLIPVFYMTEDLEATIRFGSAALVISILIDVWINRHPDPTIWTPR
                     ISWTIAMVFSGIYAAIQLFNLSLASLGLLQLGFLLELASPAGIVSNFVRELGRGSGPS
                     VEPE"
     gene            complement(549..2087)
                     /locus_tag="AC161X_04265"
     CDS             complement(549..2087)
                     /locus_tag="AC161X_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010694877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HIR4988794.1"
                     /translation="MTAVDAITLEVARNRFAAVADEMGIVLRHTSLSPNIKERADCSA
                     AIFTAEGEMLAQAEHIPVHLGSMPESVAACLRLFEPEPGVQYAVNDPFAGGTHLNDLT
                     LVAPVFVGGDHVGWVANRAHHADVGGEAPGSMPAHAVTVDQEGHRVPPTLAVSGNEWV
                     EDFSGPFVAASRTPWERRGDLAAQIGANAVGASRFAELVGNEGMERFASVSSALLDYG
                     ERRMSAAVSALPDGAFEFEDFMEHGDRDVAIRVRITIEGANLTADFTRSDGQVAANFN
                     AVEAVTRSCLYYAVRVATDPTIPTNGGCYRNIELIAPEGSVVNARFPAAVAAGNVETA
                     QRIADVLLGALAQAAPDRVPAASQGTMNNVLIGNDEFAYYETVGGGQGGRPGKPGQSG
                     IHTGMTNTENTPIESLDTHYPFRVERYTLRRGSGGVGQFAGGEGIEREIVFETAATLS
                     LMGERRRNRPWGLAGGDPGEPGEDWLIRDGDRTRLPGKTTLEVGPGDRLVLLTPGGGG
                     WGIP"
     gene            complement(2084..2809)
                     /locus_tag="AC161X_04270"
     CDS             complement(2084..2809)
                     /locus_tag="AC161X_04270"
                     /inference="COORDINATES: protein motif:HMM:NF020296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006307 - DNA alkylation repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HIR4988795.1"
                     /translation="MRFPTPTGPMSLDINAACRMVTGALAALANPEKAIGMQAYMKTD
                     MPFYGVQKPARAPIARQLVREFPPANHEEYVALVLGLWDLPHREEKYLALEVAGRFRS
                     FVDTASLPLYRRLIVEGAWWDFVDEVATHLVGHLVVHHPAEAWPEVETWIGHPDMWLR
                     RSAIICQVGAKERTDAERLFSFCEARIEENEFFIRKAIGWSLREYAKTDPEAVAEFAK
                     AHRGQLSGLSFREATKHIQHLIR"
     gene            complement(2875..4593)
                     /locus_tag="AC161X_04275"
     CDS             complement(2875..4593)
                     /locus_tag="AC161X_04275"
                     /EC_number="3.1.-.-"
                     /EC_number="3.1.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019527471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HIR4988796.1"
                     /translation="MTSRSRRWWSPAIVLLSVLALALSSLPALATQSKPKGTVDVQIL
                     AINDFHGNLQPPTGSSGRIGPSGTPEYGGAAFLATHLHNLEATNRRNTVIVSAGDMIG
                     ASPLLSALFHDEPTIEAANLWGVDFNAVGNHEFDEGIAELRRMARGRCHPVDGCQDGD
                     RFKGADFQYLSANVVSERNGKTLFPAYKIRTIGGVKIAFIGLTLEGTPNIVTPSGVAG
                     YDFLDEAETINKLIPKIRKQGAETVVVLIHEGGAQTPLPALDNIDGCTGFSGAISAIV
                     ENTNDEVDLWVSGHTHSPYNCVIDGDPVTSAYSFGRVVTDIDMKIDRRSGEPTQILID
                     NKEVTRDVTPDAAVTALIAKYDAIAAPLANRVIGSITANVVRAANAAGESALGDVIAD
                     GQLDATNDPGFGDAVVAFMNPGGIRADLTFAGSAAGEGDGNVTYGEMFTVQPFGNSLV
                     TMTLTGAQIERLLEEQLAGCTLAAARILQVSAGFTYTWTASGGVCDKVNPATIMLNGV
                     MIDPVTSYRVTVNSFLADGGDSFPILIQGTDRLGGSVDTDAFEAYFATNSPVPPGPQN
                     RITVVP"
BASE COUNT          884 a         1478 c         1419 g          847 t
ORIGIN      
        1 tggaaacgat agggcaaacc cctattcggg ttcgacgctc ggacccgagc cgcgaccgag
       61 ctccctgacg aagttggaga cgatcccagc gggggaggcc aactccagaa ggaatccgag
      121 ttggagaagc ccgagactgg ccagcgagag gttgaaaagc tgaatagcgg cgtaaatccc
      181 gctgaagacc atggcgatcg tccagctgat tctcggggtc cagatcgtgg gatcgggatg
      241 tcggttgatc cagacatcga tcaggatcga aatcactaag gcggctgatc caaaccggat
      301 ggtcgcctcg agatcctcgg tcatgtagaa gaccgggatc aggagcaagg ccagaaatgc
      361 gacagtgaaa gcctgacgga cgatggtctt cacccgccac aggttgatcg ggtcccaggt
      421 agtggagtcg tcgcgaagcc atgcgatcag agcagcaaac ccggcgagac tcaccgacaa
      481 cccggcaacc gtggtgaaga agaactgacc ttccaagtgg tgtgatcgta ccgatgaggt
      541 ccggcgactc agggtatgcc ccaaccgccg cctcccggag tgaggagcac cagccgatcg
      601 ccgggcccta cctccagagt cgtcttgccg gggagacggg tcctatctcc atccctgatc
      661 agccaatcct cgcccggttc cccgggatcg ccccccgcca gaccccaggg ccggttgcgt
      721 ctccgctcac ccatcagcga caacgtggca gccgtctcga acacgatctc tctctcgatg
      781 ccctcgccgc cggcgaactg cccgactccg ccactgcctc gccgcagcgt gtagcgctcc
      841 acgcgaaatg gatagtgggt gtcgagcgac tcgatcggcg tgttctcggt gttggtcatc
      901 ccggtgtgga tcccggactg acctggtttc cccggacgcc cgccctgacc gccacccacc
      961 gtctcgtagt aggcgaattc gtcgtttccg atcaggacgt tgttcatggt gccctgagag
     1021 gctgccggca cccgatccgg tgccgcctgg gccagcgcac cgagcagcac atcggcgatc
     1081 cgttgtgcgg tctccacatt tcccgcggcc accgccgccg ggaatcgggc attcacgacc
     1141 gatccctcag gtgctatcag ctcgatgttg cgatagcacc caccgttggt cgggatcgtg
     1201 gggtcggtgg ccacccgcac cgcgtaatag aggcaggatc gggtgaccgc ttccacggca
     1261 ttgaagttgg ccgcaacctg tccgtcactc ctggtgaaat cggctgtcag gttggctccc
     1321 tcgatggtga tccgaacccg gatcgccacg tcccggtccc cgtgctccat gaagtcctcg
     1381 aattcaaagg ccccatcggg cagagccgac acggccgcac tcattcgacg ctcgccgtag
     1441 tcgagaagcg ccgaagacac tgaagcgaac cgttccatgc cctcgtttcc caccaactcg
     1501 gcaaagcggc tcgctccgac cgcattggcg ccaatctggg cggcgaggtc tcccctccgt
     1561 tcccacggtg ttctggaagc tgcaacgaac ggcccgctga aatcctcgac ccactcgttg
     1621 cccgacaccg cgagggtcgg cgggacccga tgcccctcct gatcgaccgt caccgcatgc
     1681 gccggcatcg accccggggc ctccccaccg acatcggcgt ggtgggcccg attggccacc
     1741 caacccacgt ggtccccgcc cacgaacacc ggtgccacca gggtgaggtc gttgagatgc
     1801 gtgccaccgg cgaatgggtc gttgaccgcg tactggaccc cgggttctgg ttcgaagagt
     1861 cgaaggcagg cggccaccga ctccggcatc gagccgagat gaaccgggat gtgctcggcc
     1921 tgggccagca tctcaccttc ggcggtgaat atcgccgccg aacagtcggc ccgctccttg
     1981 atgttgggtg aaagcgaggt gtggcggagg acgatcccca tctcgtcggc cacagctgca
     2041 aaccggttgc gggcgacttc cagggtgatg gcgtcgacgg ctgtcatcga atcaggtgct
     2101 ggatgtgctt ggtcgcctcc cggaaactga gaccggagag ctggccacga tgcgcctttg
     2161 cgaattcggc tactgcttcc gggtcggtct tggcgtactc ccgcaacgac caaccgatcg
     2221 ccttgcggat gaagaattcg ttctcctcga tccttgcctc gcagaaggag aagagacgtt
     2281 ccgcatcggt gcgctccttg gccccgacct gacagatgat cgcgctccgc cgcaaccaca
     2341 tgtcgggatg gccgatccag gtctcgactt ccggccaggc ctccgccgga tgatgaacga
     2401 cgagatgtcc gacgagatgg gtggcgacct catccacgaa gtcccaccag gcgccttcca
     2461 cgatcaagcg ccggtagagc ggaagcgacg cggtgtcgac aaaacttcga aagcgcccag
     2521 ccacctcgag tgccagatac ttctcctctc gatggggcag gtcccatagc cccagcacca
     2581 gggccacgta ctcctcatga ttggcgggcg gaaactcccg aaccaactgc cttgcgatag
     2641 gggcccgggc cggtttctgc accccgtaga agggcatgtc ggtcttcatg tacgcctgca
     2701 tcccgatcgc cttttccggg ttggcgagag cggcaagagc tccggtaacc attctgcacg
     2761 cggcattgat gtcaagactc atagggccgg tgggcgtggg gaaacgcata cctgaatggt
     2821 tcgagtccgg gggcttaggc ccccggactc gattgagatc aacacagggc gaagttaggg
     2881 gacgactgtg atcctgttct gcgggccagg tggcaccggg gaattggtgg cgaagtaggc
     2941 ctcgaatgcg tccgtgtcca ctgagccacc caaccgatca gtcccctgga tgagaatggg
     3001 gaagctgtct ccaccgtcag ccaggaagct gttgacggtg acccggtagc tcgtcacagg
     3061 gtcgatcatc acgccgttca gcatgatggt cgccggattc accttgtcgc acaccccacc
     3121 actggcggtc cacgtgtaag tgaacccagc cgagacctgc agaatgcgag cggccgcaag
     3181 ggtgcagccg gccaactgct cttccagcaa tcgctcgatc tgagcacccg taagggtcat
     3241 ggtcaccaag ctgttgccga aaggctgcac cgtgaacatc tctccatagg tgacgtttcc
     3301 gtccccttcg ccggccgcac tgccagcaaa agtgagatcg gcacggatgc cacccgggtt
     3361 catgaaggcc acgacggcgt ccccaaaccc agggtcgttg gtggcatcga gttggccgtc
     3421 agcgatgaca tcgcccaagg cagactcgcc ggccgcattg gctgccctga cgacattcgc
     3481 ggtgatcgag ccgatgaccc gattagccaa gggggcagcg atggcgtcgt acttcgcgat
     3541 cagggctgtg accgccgcat ccggcgtcac gtccctggtc acttccttgt tgtcgatgag
     3601 aatctgagtc ggctcaccgc tgcggcggtc aatcttcata tcgatgtcgg tgaccacccg
     3661 tccaaacgaa taggcgctgg tcaccgggtc tccgtcgatg acacagttgt aaggcgagtg
     3721 tgtatggcca ctcacccaaa ggtccacctc gtcgttcgtg ttctcaacga tggccgaaat
     3781 cgcacccgaa aaaccggtgc aaccgtcgat gttgtccagg gccggcaatg gcgtctgagc
     3841 cccgccctcg tggatgagga cgaccacggt ctccgcgccc tgcttcctga tcttggggat
     3901 caacttgttg atcgtctccg cctcgtcgag aaagtcgtaa ccggccaccc cggagggggt
     3961 aacgatgttc ggggttccct cgagggtcag cccgatgaag gcgatcttca cgcctccgat
     4021 cgtccggatc ttgtaagccg ggaacagcgt cttcccgttg cgctcgctga ccacattggc
     4081 cgacagatac tggaaatctg cgcctttgaa tcggtccccg tcctgacatc catccacggg
     4141 atgacagcgc ccacgtgcca tacgtcgcaa ttcggcgatg ccctcgtcga actcgtggtt
     4201 gccaacggcg ttgaagtcca caccccagag gttggcggct tcgatggtcg gctcgtcgtg
     4261 gaagagggca gacaggagtg ggctcgcgcc gatcatgtct ccggccgaga cgatgaccgt
     4321 gttgcgacgg ttggtggcct cgaggttgtg aagatgggtc gcgagaaatg cggcaccgcc
     4381 gtactccggt gtgccggatg gaccgatcct gcccgaagag ccggttggtg gctgcagatt
     4441 gccgtgaaag tcattgatgg cgaggatctg gacatcaacg gtgcccttcg gcttcgactg
     4501 cgtggcaagt gccggaaggc tgctcaaggc gagagccaga acgctgagta acacgatggc
     4561 gggactccac caccgacgag agcgagaagt catatcggtc ctccttcgtt tcgcggctag
     4621 gacgccag
//
LOCUS       DBNHXU010000167         8678 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4617083, whole genome shotgun sequence.
ACCESSION   DBNHXU010000167 DBNHXU010000000
VERSION     DBNHXU010000167.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8678)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8678)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8678
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4617083"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..637)
                     /locus_tag="AC161X_04280"
     CDS             complement(<1..637)
                     /locus_tag="AC161X_04280"
                     /EC_number="2.3.1.-"
                     /EC_number="6.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF046470.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HIR4988797.1"
                     /translation="MEKLTLVEDSYPDHPAMDVAVAHATLSAVARGDIEGVFRLHVPG
                     SIVAFGRADRVQPGYPLAVRAAQAHGFGAVERLAGGRAAVFHGQTLAFSLALADNEPQ
                     GGIHSRFQMISGVMVEAFQSLGIDARIGEVRGEYCPGSWSVNVGGRVKVMGVGQRLVR
                     GAAHIGGVIVVDDGESIRNVLIPVYRALHIDWDPRTSGALADRSPGLDNSKV"
     gene            754..1515
                     /locus_tag="AC161X_04285"
     CDS             754..1515
                     /locus_tag="AC161X_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988798.1"
                     /translation="MRKAYRFLALTAAAMLLFACGGAPFASLGRRSSDWISEPTIVTT
                     TTVPITVPSVIRSDVLRWFNDTIVTTAPLDNPVAVEDEIFARRAGDLFIQASRAEMLA
                     ILPEIRFPATVPFLAEYVTSQVVFDNDGELSSDPVATFGIWSSEPYTRSRSVAQLAVL
                     EISSDPEAAAALQQPDGDVSCARFADRTTAVCESFSVEGRPIWSLTADNGTTLVWFEG
                     IYRYELFGRSFVPLQTLQQMAVESVALADLEPLGG"
     gene            1579..3441
                     /locus_tag="AC161X_04290"
     CDS             1579..3441
                     /locus_tag="AC161X_04290"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015922491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988799.1"
                     /translation="MRYDEEHDRPDIDRALLRRVWSYGRPYLSSLIGILATIFIISGL
                     GVVPPMVIRLLLDEAIPDKDIRQLSLLGLAMILVPLISAVVGVAQRWWSSRVGEGIIY
                     DLRRELFAHLQRMSLRFFTATKTGELMNRLNSDVEGSQSAITGTLMTIISNIVMVVII
                     LAVMLATDWRLTLLAVAALPLFVLPARRVARVLRRMTQQLMEQESKMSGTLQESFNVS
                     GALLVRLFGRWDEMAERFGEQAAQVRDLEVRRAMVGRGFFAALGLVAAVGTATVFWVG
                     GYLVINDQLTTGDIVMFSFLLVQLYGPLAGISNSRVEFATSLVSFERVFEVMDLEVDI
                     PDPEEAEPLVPAQGRVELRDVSFRYQSDGPEGLEAVKRFWDRDPGVAPAVASASTREW
                     AIKNASFVSEPGTLTALVGPSGAGKTTISYLVPRLYDVTEGAVLLDGHDVRNVALREL
                     AASVGVVTQETYLFHDTIAANLRYAKPTAELDELVAACQAANIHEMITKLPMAYETVV
                     GERGYRLSGGEKQRIAIARVLLEDPRILILDEATSHLDSRSEALIQEALEVVMEGRTS
                     IVIAHRLSTVRAAHQILVLDDGQIVERGDHATLVAQGGLYASLYRTQFAESASA"
     gene            complement(3443..3793)
                     /locus_tag="AC161X_04295"
     CDS             complement(3443..3793)
                     /locus_tag="AC161X_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988800.1"
                     /translation="MCEHQPTWVGRVAITACADCGQVDWFSDTGPVDPAEGMAYLFGS
                     YDLIDHLDALAAPAPTVLVYAPPSARKRRHLDAIPKNVWLRVGPSLWLSHDSQTLLLA
                     TNQQLLIDNLIRGA"
     gene            complement(3901..4410)
                     /locus_tag="AC161X_04300"
     CDS             complement(3901..4410)
                     /locus_tag="AC161X_04300"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR4988801.1"
                     /translation="MRILIATTGVLPSAPVADLCDRLRSEDGRVTVMTVVRVPRSFLE
                     ALDDDVRRSFLDDSPIDSSDAEAKAARYLEERGLRAVEPIVAALQAAGMSADVRFVEG
                     DDPAEAIINTAEDVGAELVMMGATRRLFTEEAWRSVSARVIDKSHLPVLLVPGTRIED
                     TQEVPIIET"
     gene            4484..5260
                     /locus_tag="AC161X_04305"
     CDS             4484..5260
                     /locus_tag="AC161X_04305"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4988802.1"
                     /translation="MKISVEGKVALVTGASRGIGLATALELARSGATGVTITSRKAET
                     IAEAAAELEAAGVDPEQILALPSRSDSEESADDAVAQTVERFGSCDILINNAGTNPSA
                     GPLMDVDLGALDKTWAVNLRAPLLWVRAAYRASMAKNGGSIVNVASVGGLRASAVTGA
                     YNISKAGLVHMTRQLAFELAPGIRVNAVAPAVVKTKLSEMLWTDEPMAARMHPLGRLG
                     TPEDVARAIVFLASDAADWMTGVILPVDGGVTEASSSGIA"
     gene            5272..6408
                     /gene="cofG"
                     /locus_tag="AC161X_04310"
     CDS             5272..6408
                     /gene="cofG"
                     /locus_tag="AC161X_04310"
                     /EC_number="4.3.1.32"
                     /inference="COORDINATES: protein motif:HMM:NF004884.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="7,8-didemethyl-8-hydroxy-5-deazariboflavin
                     synthase CofG"
                     /protein_id="HIR4988803.1"
                     /translation="MHPAPDPELALCYLDGVVGDEPLFAEARRLRELGHGRTITYSRK
                     VFIPLTTLCNDRCTYCTFAKPPGAGGLFLEPDEVLAVARAGDEAGCTEALFTLGDRPE
                     DRWPQAVEFLAGKGLSSTIEYVAAMSALVRSETALYPHANPGLMGDVEMKQLRPTNPS
                     MGMMLENISPRLMEPGMPHHNCPDKEPALRVETIEAAGRNKVPFTTGVLIGIGERPSE
                     IVDSLFELRRLSEETGAIQEVIIQNFRAKADTPMRWSAEPVPRWFARVVALARWIMGA
                     EANVQVPPNLTERFEIYLDAGINDWGGVSPLTIDWVNPEAPWPHLDHLRGRTESAGFT
                     LRPRLPVYPEFITPEWIHPDLYDNVVASSDDDGLAHIPEHVGAS"
     gene            6405..8270
                     /gene="cofH"
                     /locus_tag="AC161X_04315"
     CDS             6405..8270
                     /gene="cofH"
                     /locus_tag="AC161X_04315"
                     /EC_number="2.5.1.147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-amino-6-(D-ribitylamino)uracil--L-tyrosine
                     4-hydroxyphenyl transferase CofH"
                     /protein_id="HIR4988804.1"
                     /translation="MTVTFLRLRPSETIAQPAAESRRREGTTTVEIIDPNAEDLAGAA
                     AGGVLVTGSTNGDAEPAAWVVGPEDALALSSKGIPGWRLTVVGDEARESVLDGLARSG
                     AAFLRATRSTVEEMAQLVLLPGIDASVSVFVDDVVQAVRMVDLGAGDLLLRDWDVERI
                     GQLREALGSCLLLERTAFPPGVEIDEARDKLPSEVFRVWLSQIDGSGVARPRHIWAPG
                     KDLAPPVPEHRFSAEWMDSSWVAGSAPEGLSAAGDLAPILERSLEGRRPTTQEVEALF
                     GARGDQVDAVAYVADRLREQAVGDTVTYVVNRNINYTNLCYFKCGFCAFSKGPKSLNL
                     RGEPYLMGIDEIVGNAREAWARGATEVTLQGGIHPDFTGNFYVDVVRSIKAELPDMHI
                     HGFTPLEVWQGAHTLGIPVRQFLEELKEAGLGTLPGTAAEILDDEVRAHLCPDKIRTS
                     QWAEVMITAHELGLRATSTIMFGHIDHPRSWANHLEVIARIQQRTGGFTEMVPLPFVH
                     MGSPIFLRGRARPGPTWDEVVLIHAVSRIALDGLVPNIQASWVKLGLDGGRRLLDAGC
                     NDLGGTLMGEIITRSAGAEHGQEVTPQQFREAIEAAGRSPALRNTTYEILELKKG"
     gene            complement(8273..>8678)
                     /locus_tag="AC161X_04320"
     CDS             complement(8273..>8678)
                     /locus_tag="AC161X_04320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003541094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR4988805.1"
                     /translation="TLKADETGEAFHVQQAKQIREFRSAWKEAGHDFEPRVSVSRSIF
                     ALVDDRDHAYFGSRQQTTDQIGYIDANTRAIFGRSYAAEPEALIQQLAEDEAIAAADT
                     LLLTVPNQLGVDYNAHVLESILTHVAPALGWR"
BASE COUNT         1544 a         2719 c         2785 g         1630 t
ORIGIN      
        1 tgaccttcga gttgtcgaga cccggcgatc ggtcggcgag ggccccggat gtcctcgggt
       61 cccagtcgat gtgaagcgcc cgatagaccg ggatcaggac gttgcggatg ctctctccgt
      121 catcgacaac aatgaccccg ccgatgtggg cggcaccgcg caccagacgc tggccgactc
      181 ccattacctt gacccggcct ccgacgttga ccgaccagga tccggggcag tattcgcctc
      241 gcacctcgcc aatccgggcg tcgatgccga ggctttgaaa ggcctcgacc atcactcctg
      301 agatcatctg aaatcgactg tgaataccgc cttggggctc gttgtccgcc aacgccaggg
      361 aaaaggcgag cgtctgcccg tggaatacgg ccgcccgacc gccggcgagc cgttccacag
      421 cgccgaaccc gtgggcttgg gcggctctga ccgcgagcgg gtagcccggc tgaactcgat
      481 cggcgcgtcc gaaggcaacg atcgacccgg gcacatgaag acggaacacg ccttcgatgt
      541 ctccacgggc gacagcagaa agggtggcat gtgcaactgc cacgtccatg gctggatgat
      601 cggggtagga gtcctcgacc agcgtgagct tttccacaac caagaggata agtagtccac
      661 tgttaccgtt tggggaaccg cgccgtatcg ggaacgtcga tggccggttc atccgttgag
      721 ttcgcgggtg aggggcaatc gaaaggaggg tgcgtgcgaa aggcgtatcg ttttcttgcg
      781 ctgacggcag ccgccatgct cctcttcgcc tgtggaggtg cccccttcgc ctctttgggt
      841 cggcgcagct ccgattggat ctccgagccg acgatcgtta ccaccaccac ggtgccgatc
      901 accgtgccct cggtcatcag gtcggatgtc ctccggtggt tcaacgacac catcgtcacc
      961 accgcgccac tcgacaaccc tgtggccgtc gaagacgaga tattcgcccg tcgggctggt
     1021 gatcttttca tacaggccag ccgcgccgag atgctggcca tcttgcctga gatcagattt
     1081 ccggccacgg tgccgtttct cgccgagtac gtgacctcac aggtggtgtt tgacaacgac
     1141 ggtgagctgt cgtcggatcc ggtcgccacc ttcgggatct ggtcctcgga gccttacacc
     1201 aggtcgcggt cggtcgctca gttggcagtt ctcgagattt cctcggatcc agaggctgct
     1261 gccgcactgc agcaacctga tggcgacgtc tcatgcgctc gtttcgcaga tcgcacgacg
     1321 gcggtgtgtg aaagcttctc tgtcgagggg aggcccattt ggtcgctcac tgctgacaac
     1381 ggcaccacgc tggtctggtt cgaggggatc tatcgatacg agttgtttgg caggtccttt
     1441 gtccccttgc agactctgca gcaaatggct gtcgaatcgg tggcacttgc ggacctagag
     1501 cctcttgggg gataacctcg gtccaagtcc gagccgagga ggcccaccat cttccactcc
     1561 agtggctggt ggacatacat gcgttatgac gaggagcatg atcgccccga catcgatcgt
     1621 gccctgctcc gtcgtgtctg gagctacggg aggccctacc tctccagtct gattgggatt
     1681 ctggccacca tcttcatcat ctccgggctg ggcgttgtcc ctccgatggt gattcgactc
     1741 ctgctcgacg aagcgatccc agacaaggac atacgccagt tgagcctcct ggggctggcg
     1801 atgatcctgg tgccgttgat cagtgcggtg gtcggagtcg cccaacgatg gtggtcgtcg
     1861 cgggtgggag aagggatcat ctatgacctc cgtcgagagt tgttcgccca cctgcagcga
     1921 atgtctctgc ggttcttcac ggcgaccaag accggggagc tgatgaaccg cctgaacagc
     1981 gacgtggagg gttcgcagtc ggcgatcacc gggaccttga tgaccatcat ctccaacatc
     2041 gtcatggtgg tgatcatcct cgccgtcatg ctcgcgaccg actggcgact gacgcttctc
     2101 gcggtggctg ccctgccgct ctttgtgctt ccggcgcgac gggtggccag agtcctgcgg
     2161 cgcatgaccc agcagctgat ggagcaggag tccaagatgt cggggaccct ccaggagagc
     2221 ttcaatgtca gcggcgccct cctcgtccgc ctcttcggtc gatgggacga gatggcagag
     2281 cgatttgggg agcaggcggc ccaggtccga gacctcgagg tgagacgggc catggtgggc
     2341 cgagggttct tcgcggccct ggggctggtg gcggcggtcg gaaccgcaac cgtgttttgg
     2401 gtcggtgggt atttagtgat caacgatcag ctgaccacag gggacatcgt catgttctcg
     2461 ttcctcctgg tccagctcta cgggcccttg gccgggatct ccaactcacg cgtcgaattc
     2521 gctacctcgc ttgtgtcttt cgagcgggtg ttcgaggtca tggacctcga ggtcgatatc
     2581 cccgatcccg aagaggccga gcctctggtc ccggctcagg ggagggtcga acttcgtgat
     2641 gtctcgttcc gatatcagag tgacgggcct gaggggctgg aggcggtaaa gcggttctgg
     2701 gaccgggatc caggcgttgc tccggccgtg gcctcggcgt cgacccggga atgggcgatc
     2761 aagaatgcgt cgttcgtgtc cgagccggga actctgaccg ccctcgtcgg gccttcagga
     2821 gccggtaaga ccactatctc ctatctggtg ccaaggctct atgacgtcac cgaaggcgcg
     2881 gtgttgctcg acggccacga tgttcgcaac gtcgcactcc gcgagcttgc cgcttctgtg
     2941 ggcgtcgtca cccaggagac ctatctcttc cacgacacca tcgccgccaa cctccggtat
     3001 gccaagccaa ccgccgagct ggacgagctg gtagcggcct gtcaggcggc caacatccac
     3061 gagatgatta ccaagcttcc catggcgtac gagaccgtgg tcggagagcg cggctatcga
     3121 ctctcaggtg gtgagaagca gcggatcgcg attgcccgtg tcttgctcga ggatcctcga
     3181 atcctgatcc tcgacgaggc cacgtcgcat ctcgactcca gatctgaggc tctgattcag
     3241 gaggcgctcg aggtcgtgat ggagggtcgg acatcgatag tcatcgctca tcgcctctcg
     3301 actgtccggg cggctcacca aatcctggtc ctcgacgacg gccaaatagt ggaacgggga
     3361 gatcacgcca ctttggtggc ccaaggtggc ctctatgcgt cgctttaccg cacccaattc
     3421 gcggagtcgg cgtcggcctg aatcatgctc ctcggatcag gttgtcgatc aggagctgct
     3481 gattggtggc gagcaggagg gtctgggagt catgggaaag ccagaggctg ggcccgaccc
     3541 tgagccacac attcttggga atcgcgtcga gatggcgccg tttgcgtgcc gaaggtgggg
     3601 cataaacgag gaccgtcggg gcaggtgcgg caagcgcgtc cagatggtcg atcaggtcgt
     3661 agctcccgaa caaatatgcc atcccttcgg cagggtccac gggcccggtg tcggagaacc
     3721 aatcgacctg cccgcaatcg gcgcaagcag tgatcgcgac ccgacccacc caggtcggtt
     3781 ggtgctcaca caactgagaa acgctcatag agggcaagct aggaagaggc agggacaaga
     3841 aatccggcgc agccggattt ccgttatacg gtctacgtat tacgtactac tcagaacgta
     3901 ttacgtctcg atgatcggca cttcctgggt gtcttcgatc cgggttccgg ggaccagcag
     3961 cacggggaga tgcgacttgt cgatcacccg tgcggagaca ctgcgccagg cctcctccgt
     4021 gaacagccgc cgggtcgctc ccatcatgac caactcggcc ccaacgtcct cggcggtgtt
     4081 gatgatcgcc tccgcgggat cgtccccctc gacgaagcgg acatcggcag acatccccgc
     4141 cgcctgcagg gcggcgacga tcggctccac tgcgcgcaaa ccccgctcct ccaggtagcg
     4201 ggcggccttg gcttcggcgt cggatgagtc gatcggggag tcgtcgagga aggagcgtcg
     4261 gacgtcgtcg tccagggcct cgagaaacga cctggggacc ctcaccacgg tcatgaccgt
     4321 cacgcgtcca tcctcggacc gaagacggtc gcagagatct gccaccggtg cggaaggaag
     4381 gaccccggtc gtggcgatca ggatgcgcac caggctgaga ttagggtgtc gaatccggtc
     4441 gcgtcgctgc gaaagtcatc gtggatcggc ccacaggagc actatgaaga tctcggttga
     4501 aggcaaggtc gccctggtca ccggggcgag ccggggcatc ggcctggcca cagcactcga
     4561 gctcgcccgg agcggcgcca caggcgtgac catcacctca cgcaaggccg agaccatcgc
     4621 agaagccgcg gcggagctcg aggccgccgg ggtcgacccc gagcagatcc tggcgcttcc
     4681 ctcgcgttcg gactccgagg agtccgccga cgacgcagtg gcgcagactg tcgagcgttt
     4741 cggatcgtgc gacatcctca tcaacaacgc gggcaccaac ccttcagccg ggcccctcat
     4801 ggacgtcgat ctcggggcac tggacaagac atgggcggtg aacctccgcg cccctctgct
     4861 ttgggttcga gccgcatacc gggcttcgat ggccaagaac gggggttcga tcgtcaacgt
     4921 cgcctcggtc ggcggtcttc gcgccagcgc ggtgaccggg gcttacaaca tctccaaggc
     4981 gggactggtg cacatgaccc gtcagctcgc ctttgagctg gcacccggta tcagagtgaa
     5041 cgcagtcgct cccgccgtgg tcaagacaaa gctctccgag atgctctgga ccgacgagcc
     5101 catggccgct cgcatgcacc ctttgggccg attgggaacc cccgaggacg tggcgagggc
     5161 aatcgtgttc ctggcatccg atgccgcaga ttggatgacc ggagtcattt tgccggttga
     5221 cggtggtgtg accgaagctt cctcgtccgg cattgcctga attttcgacc catgcatcct
     5281 gctcccgatc ccgaactggc cctctgttac ctcgacggag tcgtcggcga tgagcccctt
     5341 ttcgccgagg cgcgtcgcct ccgagaactg ggacatggtc ggacgatcac ctacagccgc
     5401 aaggtcttca tcccgctgac cacgctgtgc aacgatcgct gcacctattg caccttcgcc
     5461 aagccacccg gcgccggcgg tctctttctc gaaccggacg aggtcctggc cgtggcgcga
     5521 gccggtgacg aagcagggtg caccgaggct ctgttcacgc tcggcgaccg cccggaggat
     5581 cgctggcctc aggctgtcga gttcctcgca ggaaaaggcc tctcatcgac gatcgagtac
     5641 gtggctgcga tgtcggcttt ggtcagatcc gagaccgcgt tgtatccgca tgccaaccct
     5701 ggtctgatgg gagatgtcga gatgaaacaa cttcgaccga ccaacccctc gatggggatg
     5761 atgctggaga acatctcacc gcggctcatg gagccgggga tgccccacca caactgcccg
     5821 gacaaggaac ctgccctgcg agtggagacg atcgaggcgg caggcaggaa caaggtcccg
     5881 ttcacaaccg gcgtcctcat cggcatcggt gaaaggccca gtgagatcgt cgactccttg
     5941 ttcgagctcc gccgactttc ggaggagaca ggggcaatac aggaggtgat catccaaaat
     6001 ttccgtgcca aggccgacac cccgatgcga tggtcggctg agccggtacc gagatggttt
     6061 gcgagggtgg tggccctggc gcgctggatc atgggagccg aagcaaacgt ccaggtcccg
     6121 cccaatctca ccgagcgttt cgagatctac ctcgatgccg ggatcaacga ctggggcggg
     6181 gtgtcacccc tgaccatcga ttgggtgaat cccgaggcac cgtggcccca cctggaccat
     6241 ctccgaggcc ggacggagag tgccggcttc acgcttcggc cccggctgcc ggtctatcca
     6301 gagttcatca cacccgaatg gatccatccc gacttgtatg acaacgttgt tgcctcgtca
     6361 gatgacgatg gccttgccca catccccgag cacgtcggag cgtcatgact gtcaccttct
     6421 tgagattgag accctccgag acgatcgccc agcccgcggc cgaaagtcga cggcgggaag
     6481 ggaccaccac tgtggagatc atcgatccca acgccgagga cctggccggg gcggccgccg
     6541 ggggagtgct ggtcaccggt tccacgaacg gtgacgccga gcccgccgcc tgggtagttg
     6601 ggcccgaaga cgcgttggcc ctttcatcca aagggattcc ggggtggcgg ctcacggttg
     6661 taggcgacga agctcgagag tcggtcctcg acgggctagc gaggtcgggg gcggcattcc
     6721 ttcgggcgac ccgatcaact gtcgaggaga tggcccaact ggtcttacta cccggcatcg
     6781 atgcgtcggt gtccgtgttt gtcgacgatg tggttcaggc cgtgcgaatg gttgacctgg
     6841 gagccggcga cctccttttg cgagactggg atgtcgagag gatcgggcaa ctccgagagg
     6901 ccctcggctc gtgccttctc ctcgagcgca ccgccttccc tccaggagtc gagatcgacg
     6961 aggcccgcga caagctccca tcggaggtct tcagagtctg gctgagccag atcgacggct
     7021 ccggtgtcgc acgaccccgc cacatctggg cgcccggcaa ggatttggcg ccgcctgtgc
     7081 cagagcaccg cttttctgca gagtggatgg actcctcgtg ggtagcgggg tcagctccgg
     7141 aaggtttatc ggcggccggt gacctggcgc cgatcctgga acggtccctc gaaggacgcc
     7201 gccctacgac ccaggaggtc gaggcgctat tcggcgcccg tggggaccag gtcgacgccg
     7261 tcgcctatgt ggccgatcgg ctccgggagc aggcggtggg ggacacggtc acctatgtgg
     7321 tcaatcgcaa catcaactac accaacctct gttatttcaa gtgcgggttc tgcgccttct
     7381 ccaagggccc aaagagcctg aacctgcgcg gagagcccta tctgatgggg atcgatgaga
     7441 tcgttggcaa tgcccgggag gcctgggccc ggggcgccac cgaggtcacg ctgcaggggg
     7501 gcatccaccc cgacttcacc ggcaacttct acgtcgacgt tgtgcggtcg atcaaggcgg
     7561 agttgcccga catgcacatc catggattca cccccctcga ggtgtggcag ggggcccaca
     7621 ctctcggcat accggtccga cagttcctcg aggagctcaa agaggccggg ttgggaaccc
     7681 ttcccggaac cgcggccgag atccttgacg acgaggttcg ggcccatctc tgcccggaca
     7741 agatccggac ctcacaatgg gcggaagtga tgatcaccgc ccacgagctc gggctgcggg
     7801 cgacctcgac gatcatgttt ggccacatcg accaccccag atcgtgggcc aaccatctcg
     7861 aggtgatcgc ccggatccag caacgcaccg gcgggttcac cgagatggtc ccccttccct
     7921 tcgtgcatat gggctcacca atcttcctgc gagggcgggc ccgacccggg cccacctggg
     7981 atgaagtcgt cctcatccac gcggtgtccc ggatcgccct cgacggcttg gtccccaata
     8041 tccaggcctc gtgggtgaag ctcgggctcg atggggggcg tcgccttctg gatgcgggtt
     8101 gcaacgacct ggggggcacc ctgatgggcg agatcatcac caggtcggca ggcgccgagc
     8161 acggccagga agtcacgcct cagcagttcc gtgaggcgat cgaagctgcg ggccgttccc
     8221 cggccttgcg caacaccacc tacgagatct tggagctgaa gaagggctga gctcagcgcc
     8281 agccgagggc cggggcgaca tgggtcagaa tgctctccag gacgtgggcg ttgtagtcga
     8341 cgccgagctg gttggggacg gtgagcaata gcgtgtctgc agctgcgatg gcctcgtcct
     8401 ccgcgagttg ttgtattagg gcctctggct cggctgcgta ggagcggcca aagatcgccc
     8461 tcgtattcgc atcgatgtag ccgatctgat cagtggtctg ttgccggctg ccgaaatagg
     8521 cgtggtcgcg gtcgtccacg agagcgaaga tgctgcggct caccgagacg cgcggctcga
     8581 agtcgtgacc ggcttccttc cacgccgagc ggaactcccg gatctgcttc gcctgctgca
     8641 cgtggaacgc ctcccccgtc tcgtcggcct tgagcgtc
//
LOCUS       DBNHXU010000168         4389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3227920, whole genome shotgun sequence.
ACCESSION   DBNHXU010000168 DBNHXU010000000
VERSION     DBNHXU010000168.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3227920"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="AC161X_04325"
     CDS             complement(<1..858)
                     /locus_tag="AC161X_04325"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CAP and S-layer homology domain-containing
                     protein"
                     /protein_id="HIR4988806.1"
                     /translation="MAESSKSGVKGRRPIVVALIVASLLLPQLALPAFAQTEGAYEVC
                     LLDAINDSRAAAGADALTMASDLVPSVREYSEWMRHHSFQHMSGSARNAILPDGTFTW
                     GENIAMWGDPSAHCDRAHDMLMNSPGHRANILNPSFRYVALGAHIDSSGEWVTELFFN
                     ANGYTPGINGHFWDDDTSIFESAIEKFAAAGITQGCNPPVNDRFCPDDYVTRGMMAAF
                     LVRGLGLTDPGNVDFIDDNGSTFESAIEKLAAAGITQGCNPPQNNRFCPNQYVTRGMM
                     AAFLVRGLEL"
     gene            1003..2067
                     /locus_tag="AC161X_04330"
     CDS             1003..2067
                     /locus_tag="AC161X_04330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4988807.1"
                     /translation="MQKPSTPILELSPGESDRALEDAHRPLLAVVACLDALIRSEDET
                     GLLDEVCRILVEEGGFHRAWVISQAEGEGESSLSIPLPIGDDSRLTLNIDCVGLDEFE
                     VELLANFAGSLSQRIVGLRAEARRDATEAQLRESLRAKDELIASIFHEMRTPLTAVVG
                     FAQLLQNEESGLSSGERTELIQSIADEGLDLTNLVEDLLTAAKAESGTLTVVSVPVDL
                     RANAAQVLETLNGDRPIELAGPSVRVAGDPGRVRQILRNLISNAIRYGGERIRVVVVD
                     GQSPSIQVRDNGVGIPEEERERIFDPYQRASSAPELSASMGLGLTISRSLARLMEGDL
                     TYVYEDSESIFELILPSLLG"
     gene            2205..>4389
                     /locus_tag="AC161X_04335"
     CDS             2205..>4389
                     /locus_tag="AC161X_04335"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HIR4988808.1"
                     /translation="MDVTEVLRDILIVLVAAKVAAELAERIGVPPVIGEIVAGILIGP
                     SLLGWVGADDQVLRTLGEIGVILLLLEVGMGMNLSELGRVGRASFGVATVGVAAPLVL
                     GTVAMQLLGHDFNTSLFVGAALTATSVGITARVFGDLRALATTEARIVLGAAVADDVM
                     GLVVLTVVVRLVTDGSVSVISVIGIIGVAVAFLLISGIVGLRLAPALFAAIERVSRST
                     GTLIALALAFTLLFAEFANAARLAPIVGAFVAGLALGRSRQRDRISRELAPVGHLFIP
                     VFFLQIGIDVDVSQFLDGQVLFIAGALLVVAVIGKLVAAVGALGTPADKWLIGLGMLP
                     RGEVGLIFATIGLQGGVLDDSLYAALLLVVLITTLVTPQLLKVRFGVVRERSAPSSRG
                     STAEAPPGGWLAVADGEVRLVATPPGHLALPLALDAAVLLGSAQPSAGLLDWLSNIDQ
                     RTAQWDRRSREALLKVIQNGNARSWRFLETTGVLDHVLPELAATLRARQADPFQLDPM
                     NTHRWTVIEELRRITQAETDLTVEFSKLAHPEWLLLAALLSEGLEGSESPVAATRRIV
                     RRLDLGARAEQEIAGLIEDGELMWSAAKRGDGLGEQSVIQVASHLDTPERARALFLLT
                     VARRGDIDRQDHERLREFHELIQEVLAHDELTGLEVRNLAGRRRAETIRLIGDRPDLV
                     RRIEEAPQSYLLRETSVAIARQVGLLEPLPGPGEARVSITTVESGP"
BASE COUNT          734 a         1310 c         1461 g          884 t
ORIGIN      
        1 tagttcgagg cctcgaacca ggaaggcggc catcatcccc cgagtcacgt attgattggg
       61 gcagaaccgg ttgttctgcg gtgggttgca cccttgggtg atgccggcgg ctgccagttt
      121 ctcgatagcg ctttcaaagg tcgatccgtt gtcgtcgatg aagtcgacgt tgccgggatc
      181 ggtgagaccg aggccgcgga cgaggaaggc ggccatcatc ccccgcgtca cgtagtcgtc
      241 agggcagaag cggtcgttga ccggtgggtt gcagccttgg gtgatgccgg cggccgcgaa
      301 tttctcgatt gcgctctcga agatggaggt gtcgtcgtcc cagaagtgcc cgttgatccc
      361 cggggtgtag ccgttggcgt tgaagaacaa ctcggtcacc cactcgccag aggagtcgat
      421 gtgggctcca agggcgacgt agcgaaaact cggattgagg atgttggccc ggtgccctgg
      481 ggagttcatc agcatgtcgt gggcccgatc acagtgggcg gacggatccc cccacatggc
      541 gatgttctca ccccaggtga aggtgccgtc ggggagtatt gcgttgcggg ccgacccgga
      601 catgtgctga aagctgtggt ggcgcatcca ctctgagtac tcccgcacgg agggaacgag
      661 gtcagaggcc atcgtcaggg cgtcggcccc agcggcggct cgggagtcat tgatggcgtc
      721 gaggagacag acctcatagg caccctcggt ctgggcgaac gctgggagcg ccagctgagg
      781 gagcagcaag gacgcgacga tgagggcgac cacgatcggg cgccgacctt tgactccgct
      841 tttggacgac tccgccatag gtccctgtta gaggcagttc ccaatagatc ggccgttcaa
      901 acctctcgat tgagcgtttt ggggtcgtaa tcggtgatgt cacagagccg tctggcgtct
      961 caagcggccg cccggcggct ccgatgtttt ctagtgatat cgatgcagaa accaagcact
     1021 ccgattctcg aactgtctcc aggagagagc gacagagctc tggaggacgc ccataggccg
     1081 cttctggcag tcgtggcatg cctcgacgcg ctgatccggt cggaagacga aacgggcctc
     1141 ctggatgagg tttgccggat cctggttgag gagggcgggt ttcatcgtgc gtgggtgatc
     1201 tctcaggcag agggtgaggg agagtcgagt ctctcgattc cgctccccat cggagatgac
     1261 tcgcgattga cgctcaacat cgactgcgtc ggattggatg agttcgaggt cgagcttctg
     1321 gccaacttcg cggggtcact ctcgcagcgg atcgtggggc tccgggcaga ggctcggcgc
     1381 gacgcgaccg aggcccaact gagagagtca cttcgcgcca aggatgagct gatcgcctcg
     1441 atctttcatg agatgcgtac cccgctcaca gctgtcgtcg gctttgccca gctgctccaa
     1501 aacgaagaat caggcctctc ctcgggcgag cggaccgaac tgatccagtc gatcgcggac
     1561 gagggcctgg acctgacaaa cctcgttgag gacttgttga ctgcggccaa ggccgagtca
     1621 ggaaccttga cggtggtcag cgttcccgtc gacctgcggg caaacgctgc ccaggtcttg
     1681 gagaccctca acggtgaccg gcccatcgag cttgccgggc cctcggttcg ggtcgcgggt
     1741 gatccaggcc gggtgcgcca gatcctccgc aatctcattt ccaacgccat tcgatacggt
     1801 ggggagcgga ttcgggtggt tgtcgtggat ggccagagcc ccagtattca ggtccgtgac
     1861 aatggggttg gcatacccga ggaagagcgt gaacggatct tcgaccccta tcagcgtgcc
     1921 agctcggcgc ccgagctcag cgcttcgatg ggtcttggcc tcaccatctc acgcagtctg
     1981 gcccgtctca tggaaggcga cctcacctac gtctatgagg acagcgagag catcttcgag
     2041 ttgatccttc ccagcctcct cggctgagag acaccgtcgc gccggccccg cagcattaat
     2101 cagcgcgaaa caaagccgaa acgacgaatt cttagcgttc cagcggtgcg cactggacca
     2161 aaccgggtga gacatggccg gtcggccact ggctcgggag cctgatggac gtcaccgaag
     2221 tcctccgcga catcctgatc gtcctcgttg ctgccaaggt cgccgccgag ttggccgagc
     2281 gaatcggcgt cccgccagtg atcggcgaga tcgtcgccgg aatcctcatc gggccctcgc
     2341 tgctgggctg ggtcggtgcc gacgatcagg tgttgcgaac cctcggcgaa atcggtgtca
     2401 tcttgttgct gctcgaggtg ggaatgggga tgaatctcag tgagctggga agggtgggca
     2461 gggcttcatt tggcgtggcg acggtcggtg tggctgctcc ccttgtcctg ggcaccgtcg
     2521 cgatgcagct cctcggtcac gatttcaata cctccctgtt cgtgggcgcg gctctcaccg
     2581 ccaccagcgt tgggataacg gcccgggtgt tcggtgacct gcgggctctc gccacgactg
     2641 aggcccggat cgtgctgggg gcggcggtgg ctgacgatgt catgggattg gtggtcctga
     2701 ccgtggtggt ccgcctggtc actgacggct ctgtatcggt gatatcggtg atcgggatca
     2761 tcggggtcgc agttgccttc ttgctcatca gcgggatcgt cgggctccgg cttgcccctg
     2821 ctctctttgc ggcgatcgaa cgcgtgtccc gctcgacggg gacgttgatc gccctggcac
     2881 ttgccttcac gcttttgttc gccgagttcg ccaacgccgc ccggctggct cccatcgtcg
     2941 gggctttcgt ggctggcctc gctctgggtc ggagccgtca gagggaccga atcagtcggg
     3001 aattggcgcc cgtgggccat ctcttcatcc ctgtcttctt ccttcagatc gggatcgatg
     3061 tcgatgtatc ccagtttctc gatggccaag tgcttttcat cgctggcgcc cttctcgtgg
     3121 tcgcagttat tggaaagctg gtggcggcgg ttggcgcctt gggcacgccg gcggacaagt
     3181 ggctgattgg cctggggatg ctccctcgtg gggaggttgg cctcatcttc gccaccatcg
     3241 gcctccaggg cggggtgctc gacgactccc tctatgccgc cctgctgctc gtggtcctga
     3301 tcaccactct ggtcacgccc cagctgttga aagtgcgctt cggcgttgtt cgcgaaaggt
     3361 cggcgccgag ctctcggggg tcgacggccg aggcgcctcc gggtggctgg ctcgcggtcg
     3421 ctgacggtga ggttcggttg gtggcgacac cccccgggca cctggccctt cctctcgctc
     3481 tcgatgcggc ggtgctgctc ggttccgccc aaccctcggc gggccttctc gactggctgt
     3541 cgaacatcga ccagcgcacc gcccaatggg atcgaaggtc gagagaggcc ctgttgaaag
     3601 tcatccaaaa tggcaacgcc agatcgtggc gctttctgga gacgacgggg gttctcgatc
     3661 acgtgctacc cgagctcgct gccacattgc gggcccgcca agccgatccg ttccaactcg
     3721 acccgatgaa cactcaccgt tggacagtca tcgaggaact gcgcaggatc acgcaggcag
     3781 aaacagacct aacagtcgaa ttctcgaagc tcgcccatcc ggagtggcta cttcttgcgg
     3841 ccttgctttc cgagggtctg gagggcagcg aatctcccgt cgcagcgacc aggcggatag
     3901 tgcggagact cgatcttggc gccagggcgg agcaagagat cgcaggcttg atcgaagacg
     3961 gtgagttgat gtggtctgcg gccaagcgag gcgacggact cggcgagcaa tcggtgatcc
     4021 aggtggcatc acatctcgat accccggagc gggcccgggc gctgttcctt ctgacggttg
     4081 ctcggagagg cgacatcgat cgtcaggacc acgaacgact gcgcgagttc cacgagttga
     4141 tccaggaagt cctcgcccat gacgagttga ccggcctcga agtgaggaac ctcgcgggaa
     4201 ggcgtcgggc agagaccatc cgcctgatcg gtgatcggcc cgacctggtc cgtcggatcg
     4261 aagaggctcc ccagtcttac ctgctgcgtg agacctcggt tgccatcgcc cgtcaggtcg
     4321 ggctactgga gccacttccc gggcctgggg aggcgagggt ttccataacg accgtggaga
     4381 gcgggcccg
//
LOCUS       DBNHXU010000169         1833 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4220990, whole genome shotgun sequence.
ACCESSION   DBNHXU010000169 DBNHXU010000000
VERSION     DBNHXU010000169.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1833)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1833
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4220990"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1796)
                     /locus_tag="AC161X_04340"
     CDS             complement(<1..1796)
                     /locus_tag="AC161X_04340"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015099106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR4988809.1"
                     /translation="MDPLAGFNPATRTWFEASFAEPTPAQQLGWPAIAGGAHTLIHAP
                     TGSGKTLAAFLWTLDRLLSEPQPERSARCRVLYISPLKALAHDVERNLRAPLAGIRHA
                     SQRLFPEPLPELETFLRTGDTPAEDRRRMQRQPPDILITTPESLYLILTSAARVILEP
                     VRWVIVDEVHAIAGTKRGAHLALSLERLEEITEVAPQRIGLSATQRPLDTIARFLGGG
                     TIETGIWTARPVTVIDVPHTRELDIEVVVPVEDMTEPGTPDPLDPDRAPSRSIWPAVY
                     PELLEAIRAHNSTIVFANSRRLAERICAEVNNLAGEEIARAHHGSVSREHRLQIEDAL
                     KRGELKAVVATSSLELGIDMGAVDLVIQVEAPTSVASGLQRVGRSGHQVGGISKAKVF
                     PKYRGDLLVATVLTGEMAKRNVETTRIPANPIDVLAQQIVAEVVSRETTADRLFDLTR
                     RAAPYSELSRGVFDATLDMLAGRYPSDLFAELRPRINWDRVSGEITPRPGSRQLAVTN
                     PGTIPDRGLYRVVLPDGSRVGELDEEMVYESREGDAFLLGSSSWRITQITHDRVEVVP
                     APADTAARMPFWHGDSLGRSLETGKAVGRFVR"
BASE COUNT          315 a          555 c          649 g          314 t
ORIGIN      
        1 tcccggacaa accggcccac tgcttttccg gtctccaagg accggccgag cgagtcaccg
       61 tgccagaaag gcatgcgggc ggcggtgtcg gcgggtgcag gcaccacctc cacccggtca
      121 tgggtgatct gcgtgatccg ccacgagctg gagccgagca ggaaggcatc gccctcccgg
      181 gactcgtaga ccatctcctc gtcgagctct cccacccggc tgccgtcggg cagaaccacg
      241 cgatagaggc cgcggtcggg gatggtcccc ggattggtga cggccaattg cctcgaccca
      301 gggcgggggg tgatctcgcc gctaacccga tcccaattga ttcggggccg gagctctgcg
      361 aagaggtccg aggggtatcg cccggcaagc atgtccagag tggcgtcgaa cacgcccctg
      421 ctcaactcgg agtacggagc ggcgcgccgg gtcaggtcga agaggcgatc ggcagtggtt
      481 tccctgctca ccacctctgc cacgatttgc tgggccaaga cgtcgattgg gttcgccgga
      541 atccgggtgg tctccacgtt ccgcttggcc atctccccag tgagaacagt tgcgacaaga
      601 aggtctcccc gatacttggg gaagactttg gccttggata ttccaccgac ttgatgcccc
      661 gaccggccga cccgctgcag acccgaagcg accgaggtcg gcgcttcgac ctggatgacc
      721 agatcgaccg cgcccatgtc gatgcccaat tcgagtgatg aagtggccac cacggccttc
      781 agctcacctc gtttcagggc gtcctcgatc tggagacggt gctcccgcga gaccgacccg
      841 tggtgagccc tggcgatttc ctcaccggcg aggttgttca cctcggcaca gatccgctcg
      901 gcgagccggc gtgaattggc aaagacgatt gtgctgttgt gcgctctgat cgcctcgagc
      961 agttcggggt agaccgccgg ccatatcgac ctggaaggtg cccggtccgg gtcgagggga
     1021 tccggagttc cgggttcggt catgtcctcg accggcacga cgacctcgat gtccagctcg
     1081 cgggtgtgag ggacgtcgat gacggttacc gggcgcgccg tccaaatccc ggtctcaatg
     1141 gttccccctc ccaagaagcg ggcaatggtg tccaggggcc gctgggtggc ggacagcccg
     1201 atgcgttgtg gcgcgacctc ggtgatctcc tcgagtcgct ccagggacag cgccaggtgg
     1261 gcgcccctct tggtgccggc gatggcgtgg acctcatcga cgatcaccca gcgaaccggc
     1321 tcgagaatga cgcgggcggc cgaggtgagg atgagataga gggattcagg cgtggtgatc
     1381 aggatgtccg gcggctggcg ttgcatgcgc cgtctgtctt cggccggggt gtcaccggtc
     1441 cggaggaaag tctcgagttc gggtaggggc tcgggaaaca gcctctgcga agcgtggcgg
     1501 atcccggcga gaggagcgcg aaggttgcgc tctacgtcat gagccagtgc cttgagcggc
     1561 gagatgtaga gcacccggca gcgggcagat cgctcaggct ggggctcgga aaggaggcgg
     1621 tcgagggtcc agaggaaggc ggccagggtc ttgccggatc cggttggggc gtggatcaga
     1681 gtgtgggcgc caccggcgat cgcaggccat ccgagttgct gagccggcgt cggctcggcg
     1741 aaagatgctt cgaaccaggt gcgggtggcc ggattgaaac cggcgagtgg gtccacaccg
     1801 ggaaggtatc aggagagaag gacaagctcg ccg
//
LOCUS       DBNHXU010000170         2263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1047619, whole genome shotgun sequence.
ACCESSION   DBNHXU010000170 DBNHXU010000000
VERSION     DBNHXU010000170.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2263
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1047619"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(9..461)
                     /locus_tag="AC161X_04345"
     CDS             complement(9..461)
                     /locus_tag="AC161X_04345"
                     /inference="COORDINATES: protein motif:HMM:NF019928.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HIR4988810.1"
                     /translation="MSTETIPAVLRSVEVSIPPERAFDVFTRRMGEWWPLHTHSIGEE
                     RAVSVRFEGWTGGKVVEIVDDGTEWEWAEVLAWEPPHRLVLAWHPTENPTVSTEVEVR
                     FTGFEGGTMVELEHRGWERLGDLAAAGRVDYDQGWLPVLGLYQGLAND"
     gene            complement(458..763)
                     /locus_tag="AC161X_04350"
     CDS             complement(458..763)
                     /locus_tag="AC161X_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009155038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR4988811.1"
                     /translation="MLATAQFDALGDATRRDVMGILLERPRSVGEIAARLPVSRPAVS
                     QHLRVLEGSGLVTHRAEGTRRIYSTSASGLAELRNAIEQMWDDTLARFAAAAEEENS"
     gene            complement(803..1969)
                     /locus_tag="AC161X_04355"
     CDS             complement(803..1969)
                     /locus_tag="AC161X_04355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="bactofilin family protein"
                     /protein_id="HIR4988812.1"
                     /translation="MRYRSKSLVLAVLVSGFLLALGGTAFAAETSSSDLVIIPEGDTV
                     SDDLYATGLKILVEGVVDGDLIAFAAEEVVISGQVTGNVIAIAPKVTIEGSVTKSVRA
                     TGRELDVTGDIGGDVVAAVASATFGSESEVTGDVVIWGLTVNAAGSIGGDLSGTQRRV
                     SLEGEVGGDVNLSVGRLTITGPLTVGGDLDYRSGVEASGLDQATVGGAVVHKTPVPPN
                     IRIRALTLLTRILVVLGLSAAALLVAWGWPERTAKAGVEAGTRPLRALGRGALVLLTP
                     LLLAGLAALIVAVAPASASLPLLAIFVPLILVTAGIILVMCLVAGIPAVIVLGRLLPG
                     DRGMFGAIAMGSLLAGVVWMIPLVGWIVPLVILPWGLGAWLLTFRSEAKAPSAS"
     gene            complement(1953..>2263)
                     /locus_tag="AC161X_04360"
     CDS             complement(1953..>2263)
                     /locus_tag="AC161X_04360"
                     /inference="COORDINATES: protein motif:HMM:NF012581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="proton-conducting transporter transmembrane
                     domain-containing protein"
                     /protein_id="HIR4988813.1"
                     /translation="ILLLGMAGLPLTSGFIAKFGVFSDAWANEYQWLVVVAVLASVIA
                     FAFYIRLIVVMYMDEGEGERFKVVGSVRWVLGLAVLATLVWGIFPASLLQLAADALPL
                     "
BASE COUNT          470 a          767 c          681 g          345 t
ORIGIN      
        1 catggtggtc aatcgttggc gaggccctgg tagaggccta ggacgggcag ccagccttgg
       61 tcgtagtcga cacgccctgc cgctgccagg tcgccgagac gctcccagcc ccgatgctcc
      121 agctcgacca tggtgccgcc ctcgaatccg gtgaagcgca cctcgacttc ggtagagaca
      181 gttggattct ccgtggggtg ccatgcgagg accagcctgt gtggcggctc ccaggcgagc
      241 acctcggccc attcccactc cgtgccgtcg tccacgattt cgacgacctt gcctccggtc
      301 caaccctcga acctcacgct gaccgctcgc tcctcgccta tcgagtgggt gtgtaatggc
      361 caccactcgc ccatgcggcg ggtgaacaca tcgaaggcgc gctcgggcgg aattgaaacc
      421 tcgaccgagc ggagcaccgc cggaatcgtc tctgtgctca tgaattctcc tcctctgcag
      481 ccgcggcgaa ccgggccagc gtgtcgtccc acatttgctc gatcgcatta cggagctcgg
      541 ccaaaccgga agcggaggtc gagtagatgc gtcgcgttcc ctcggctcgg tgggtcacca
      601 accccgatcc ctccaacacc ctgaggtgct gagagactgc gggacggctg accggaagcc
      661 gggccgcgat ctcaccaacc gaccgtggac gctcgaggag gatccccatg acatcgcgtc
      721 tggtggcatc tccgagggcg tcgaattggg cggtggcaag cacgagacct agtgtaagtc
      781 acaacttacg gtaagtcaag acttacgatg ctgaaggtgc tttcgcctca gacctgaatg
      841 tgagcagcca tgcgccgagc ccccagggga gaatcaccag tggcacgatc caaccgacga
      901 gggggatcat ccagacaacc ccggccagaa gtgatcccat ggcgatcgcc ccgaacatcc
      961 cccggtctcc ggggaggaga cgtcccaaga caatcacggc ggggatcccg gcgacgaggc
     1021 acatgacgag gatgatcccg gcggttacca ggatcaaggg gacgaagatc gccagcaatg
     1081 gaagactggc ggaagcaggt gccaccgcaa cgatgagcgc agccaacccc gcgagcagga
     1141 gcggggtgag gagaaccaga gcccccctcc ccaaggccct caatggcctg gttccagcct
     1201 cgacgcccgc tttggcggtg cgctcgggcc aaccccacgc caccagcagg gccgcagcgg
     1261 aaagaccaag caccaccaag atcctcgtga ggagggtgag agctcggatc ctgatgttgg
     1321 ggggcaccgg ggtcttgtgg accacagcgc cacctacggt cgcctgatcc aagcccgaag
     1381 cctccactcc cgatcggtag tcgagatccc cacccaccgt aagcggtccg gtgatcgtga
     1441 gcctgccgac actcaggttg acgtctcccc ccacctcacc ctcgagcgac acccggcgct
     1501 gggttccgct caagtccccg ccgatcgagc cggcggcatt cacagttagc ccccagatga
     1561 cgacgtcgcc ggttacctcg gactccgagc cgaacgtggc gctggcgact gcggccacga
     1621 cgtcgccgcc gatgtcgccc gtcacgtcca actccctacc tgtggcccgc accgatttcg
     1681 tgaccgagcc ctcgatggtg accttggggg cgatggcaat gacatttcct gtcacctgcc
     1741 ctgagatcac aacctcctcg gccgcaaagg cgatcagatc gccatcgacg accccttcga
     1801 cgaggatctt gagccccgtg gcatagagat cgtcggagac ggtgtcgccc tccggaatga
     1861 tgaccagatc ggacgacgac gtctctgcag cgaatgcggt gcctcccaga gctaggagaa
     1921 acccggagac cagaaccgcc aggaccagtg acttagagcg gtaacgcatc cgcagccaac
     1981 tggaggaggg aggcggggaa gatgccccac accagagtgg ccaggacggc gaggcccagg
     2041 acccaccgca ccgagccaac gactttgaac ctctccccct cgccctcgtc catgtacatc
     2101 acgacgatga gccggatata gaaggcaaaa gcgatcacgg acgcgagcac cgcgaccacc
     2161 acgagccatt ggtattcgtt ggcccaggca tccgaaaaga caccgaactt tgcgataaac
     2221 ccagaggtga gcggcagtcc ggccattccg agcagaagaa tcg
//
LOCUS       DBNHXU010000171         2602 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2832307, whole genome shotgun sequence.
ACCESSION   DBNHXU010000171 DBNHXU010000000
VERSION     DBNHXU010000171.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2602
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2832307"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(292..1029)
                     /locus_tag="AC161X_04365"
     CDS             complement(292..1029)
                     /locus_tag="AC161X_04365"
                     /inference="COORDINATES: protein motif:HMM:NF053169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF8083 family protein"
                     /protein_id="HIR4988814.1"
                     /translation="MIKTAYLRVYSPEVADGDDPVPGFVRSYGLLSETEADGDWAVDW
                     NGRRLVCPRNLRLRVLESTVAFANAFRGMGAGLIPEEAARSADQELRRYREEHPSHRS
                     HVLTSTWHVPVRWFTAFVSDDKELYQIGGQARLRYRVDLLVARERVEHALEVLRGLQI
                     FQGPAEELGQLAEWLDLFPDDSMLELDYSNVSELFDPQDLVFDESVDLVQQSVNALAE
                     GDMLTAGENYGRVVSRWAPAFSVTFSN"
     gene            1072..2151
                     /locus_tag="AC161X_04370"
     CDS             1072..2151
                     /locus_tag="AC161X_04370"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017150925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR4988815.1"
                     /translation="MVFDQVAREGHEQIVYGYDPVSGLRTIIAIHSTALGPALGGTRF
                     YPYESEEAALTDVLRLSKGMTYKAAAAGVDVGGGKAVIIGDPRTDKSERLLRAYGRIV
                     DSLRGRYITAEDVGTTTTDIDVIRRETKWALGTSVADGGAGDPSPVTARGLFNAARAV
                     AEFISGDADLAGRRFAVQGVGKVGAAFVQLLVEARAEVIVSDAFESATKAVVENFGVK
                     SVDPDEIHRVACDFYSPCALGAGLNETTIPQLNCQAVVGSANNQLATDEDGDRIAERG
                     IVYAPDFVVNAGGLINVYDELLGYSKMRALHRVDGIFEATKTILEISKREAITPNQAA
                     VKVAEARLKDIGDLRRFRRSGDDRN"
     gene            2154..>2602
                     /locus_tag="AC161X_04375"
     CDS             2154..>2602
                     /locus_tag="AC161X_04375"
                     /inference="COORDINATES: protein motif:HMM:NF012880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HIR4988816.1"
                     /translation="MSHRSYGMDFDRNLHHGELGLTDEQMVDMYRLMLMSRRVDDRMF
                     ALQRQGRAPFVVGSSGHEAVQVASVFALDRSKDWILPYYRDMGVALAWGFTPEDIFLG
                     AFAKKSDPMSGGRQLPGHWSSPELRVLTQSSVIGTQYPHAVGVAQAIV"
BASE COUNT          506 a          818 c          828 g          450 t
ORIGIN      
        1 gaggtggcga ctgttgtcgt tgtcatcgtc atttcctcaa tttgggcaac aaaaaacccg
       61 catcctgtgc cggtgcgggt ctggtgtctg tcttgttact tgttcaggac atcactaccc
      121 aaccggcgcg gcggcacaca tacatgcgca gctgcagacg cggtgggcgg agtgataggt
      181 cattgctagg gatggtaggc gggtttggtg gttcgtcaag acgcggcgcc gtaattggga
      241 tctgtagggg tcatatacgg acctgtgcgt cccaattaca gaagggggaa gtcagttact
      301 gaaggtcaca gagaaggcgg gcgcccagcg ggagaccacc cggccgtaat tctcgcccgc
      361 ggtgagcatg tcaccctcgg ccagggcatt gaccgactgc tgcaccaggt cgaccgactc
      421 gtcgaaaacc agatcctggg ggtcgaagag ctcggatacg ttcgagtagt ccaactcgag
      481 catcgagtcg tccgggaaaa ggtccagcca ctccgcaagt tgccccagct cctcagccgg
      541 cccctggaag atctgtaacc cccgcaacac ttcgagggca tgctccaccc gctcccgcgc
      601 gaccaggaga tcgacccgat accggagacg ggcctggcca ccgatctgat aaagctcctt
      661 gtcgtcggat acgaaggcgg tgaaccagcg taccgggacg tgccaggtcg aagtgagaac
      721 gtgggaacga tgggagggat gctcctcgcg atagcggcgg agctcctgat cggccgagcg
      781 ggcggcttcc tctgggatca gcccggcccc catccctcga aaagcgttgg cgaaggccac
      841 tgtcgactcg aggacccgca gcctcaggtt ccgggggcac accagacgtc gaccgttcca
      901 atccacggcc cagtcaccgt ccgcctcggt ctcggacaaa agcccgtagg aacggacgaa
      961 accgggaacc gggtcgtcgc cgtcggcgac ctctgggctg tagaccctga ggtaggcggt
     1021 cttgatcacg ccctcatgct cgcagaggtc gatgggtagt atcgaaggac ggtggtattc
     1081 gaccaggtcg cccgggaagg tcacgaacag atcgtctacg ggtacgaccc ggtatccggc
     1141 cttcgaacca tcatcgccat ccactccacg gctctcggtc ccgctctcgg cgggacccgc
     1201 ttttatccat acgagagcga ggaagcggcc ctcaccgatg tgctcagact gtccaagggc
     1261 atgacctaca aagcggcagc ggccggggtc gacgtcggag gcgggaaagc cgtgatcatc
     1321 ggcgaccccc gaaccgacaa gagcgagcgc ctgcttcggg cctatgggcg gatcgtcgac
     1381 agcctgcggg gccgatacat cactgccgag gacgtcggca ccactaccac cgacatcgac
     1441 gtcattcggc gcgagaccaa atgggccctc ggaacctcgg tggcggatgg gggagccggc
     1501 gacccgtcgc ccgtgacagc gcgcggtctg ttcaacgcgg cgagggccgt cgccgagttc
     1561 atttcgggcg acgcggatct cgccggccgc aggtttgcgg ttcagggcgt tggcaaagtg
     1621 ggcgccgcct tcgttcagct cctcgtcgag gctcgcgccg aggtcatcgt ctctgacgcc
     1681 ttcgagagcg ccaccaaggc tgtcgtcgag aacttcgggg tgaagagcgt cgaccccgac
     1741 gagatccatc gcgtcgcctg tgacttctac tcaccatgtg ccctcggagc cgggctcaac
     1801 gaaaccacga tcccccaact gaattgccag gcagtcgtag gcagcgccaa caaccagttg
     1861 gccaccgacg aggatggcga tcggatcgcg gagaggggaa tcgtctacgc ccccgacttc
     1921 gtggtgaacg ccggcgggct gatcaatgtc tacgacgaac tcctcggcta ctccaaaatg
     1981 cgggccctgc atcgggtaga tggaatattc gaggcaacca agaccatcct cgagatatcc
     2041 aaacgcgagg cgatcactcc caaccaggcc gcggtgaagg tggcggaggc gaggctcaag
     2101 gacatcggtg acctgcggcg gttccggaga agcggagacg accgcaattg acgatgtccc
     2161 atcgctccta cggcatggat ttcgacagaa acctgcacca tggggagctc ggactgaccg
     2221 acgagcagat ggtcgatatg taccgcctga tgctgatgtc acgccgcgtc gacgaccgca
     2281 tgttcgcttt gcaacgccag ggtcgggcac cattcgtggt cggatcgtcg ggtcacgagg
     2341 cagtccaggt ggcctcggtg tttgcactcg accgctccaa ggactggatc cttccctatt
     2401 accgggacat gggggtggcg ctggcgtggg gattcacccc tgaggacatc ttcctcggtg
     2461 ccttcgccaa gaagtccgac ccgatgtccg gcggccgcca gctgcccggc cattggtcga
     2521 gccccgagct cagggtcctc acccagtcct ccgtgatcgg cacccagtac ccgcacgcag
     2581 tcggtgtcgc ccaggccatc gt
//
LOCUS       DBNHXU010000172         1823 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5412366, whole genome shotgun sequence.
ACCESSION   DBNHXU010000172 DBNHXU010000000
VERSION     DBNHXU010000172.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1823)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1823
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5412366"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1522)
                     /locus_tag="AC161X_04380"
     CDS             complement(<1..1522)
                     /locus_tag="AC161X_04380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018992447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HIR4988817.1"
                     /translation="MIFTPSGRRGSFPDGTTVLEAARALGVDLDSVCGGRGLCGRCQV
                     VVGSRPGVEARDDRLSPPGPIEIDYQGKRPLAAGQRLGCAAHIQDDVVIDVPADSQVH
                     RQVVRKRAGVSALPVDPVVRLHYVEVAEPDLASPTGDLERLMVALSEQWDLTGLSVDH
                     RVLAGLQTVLRRGKWAVTVAVRQARDLIAIWPGFRDLALGVAFDVGSTTIAGHLCDLT
                     SGAVLASAGRMNPQIGFGEDLMSRVSYVMMNEDGASRLTTVVRQALDELVGELVAEGG
                     VDRADVLELTLVGNPIMHHLALGLDPRELGTAPFALVTTSSLSTPAAELGLDVNPGAR
                     VYTLPCIAGHVGADAAGAVLSEGPHRSDTLQLLVDVGTNAELVLGNKERLLAASSPTG
                     PAFEGAQISSGQRAAPGAIERVRIDKATLEPRFSVIGQEVWSDEPGFDFGSVTGICGS
                     GIIEAIAGLFLAGVIDTDGVIREPSEPSPRVVPSERTFSYVLCTEPRIEVTQSDVRA"
     gene            complement(1541..>1823)
                     /locus_tag="AC161X_04385"
     CDS             complement(1541..>1823)
                     /locus_tag="AC161X_04385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltetrahydrofolate cobalamin
                     methyltransferase"
                     /protein_id="HIR4988818.1"
                     /translation="ELGVNTTCGASNISFGLPNRAGINAAYLAMVMGAGMTSAITNPL
                     EAEIRQAILAADVLNGHDPNCSTWIRTYREPPAAGERGRARENRRRSTG"
BASE COUNT          332 a          620 c          579 g          292 t
ORIGIN      
        1 cggcgcggac atcgctctgc gtgacctcaa tccggggctc agtgcagagc acgtagctga
       61 aggttcgctc cgagggcacc acccgcggag aaggttcaga cggttcgcgg atcacaccat
      121 cggtgtcgat gacgcctgcc aggaacaacc cggcgatcgc ctcgatgatc ccggacccgc
      181 agatcccggt gactgagccg aagtcgaaac cgggctcgtc cgaccagacc tcctgtccga
      241 tcaccgagaa ccggggctcc aacgtcgcct tgtcgattcg aacccgttcg atggcgcctg
      301 gcgctgcccg ttgcccagat gagatctgcg ctccctcaaa agccgggccg gtcgggctcg
      361 atgccgccag gaggcgctcc ttgttgccaa gcaccaactc ggcgttggtg cccacatcga
      421 ccagcagctg aagggtgtca gatcggtgcg ggccttcgga gagaacggct ccggccgcgt
      481 cggccccaac atgaccggca atgcagggga gggtgtagac ccgggcaccg gggttcacgt
      541 cgagtcccag ctccgccgcc ggagtgctca acgacgatgt ggtcaccaaa gcgaaaggtg
      601 cggtgccgag ctcgcgcgga tccagcccca gagccaggtg gtgcatgatc gggttgccca
      661 ccagagtcaa ttcgaggaca tcggctcgat ccacgccgcc ctctgcgacc aactccccga
      721 ccagctcgtc caaggcctga cgcacgaccg tggtcaggcg ggaggcgccg tcctcattca
      781 tcatcacgta ggagacccgg ctcatcaggt cttccccgaa gccgatctgg ggattcatcc
      841 ggccggcgga agccaggacc gcaccgctgg tcaggtcaca gagatgcccg gcaatggtgg
      901 ttgaaccgac gtcgaaggca acgccaaggg ccaggtcccg aaagccgggc cagatcgcga
      961 tcaggtctct tgcctggcga acggccacag tgaccgccca ctttcctcga cgtaggacag
     1021 tctgcagtcc ggcaaggact cgatggtcga cagagagacc ggtcaggtcc cactgttcgg
     1081 aaagggcaac catgaggcgc tcgagatcac cagtcgggct ggcgaggtcg ggctcagcca
     1141 cttcgacgta gtggaggcgg acgaccggat cgaccggaag ggccgacacg cctgcccgct
     1201 tgcggaccac ctgccggtgg acctgactat ccgcggggac atcaataacg acgtcgtcct
     1261 ggatgtgggc cgcgcaaccc agccgctgtc ccgcggcgag cggccgcttg ccctggtagt
     1321 cgatctcgat cggccccggt ggcgacagtc ggtcgtcgcg ggcctccacc cccggcctgc
     1381 ttcccaccac cacctgacat cgcccgcaca acccgcggcc accgcatacc gaatcgaggt
     1441 caacgccaag cgcccgcgcc gcttcgagaa ccgtggtgcc atcgggaaac gaccctctcc
     1501 gccccgaggg cgtgaagatc acctgattgg tcacggcgat tcagccggta ctacgacggc
     1561 ggttctcacg ggcgcgccct ctctcgccgg ccgccggagg ctctcgatag gttcgtatcc
     1621 aggtcgagca gtttgggtcg tggccgttga gcacgtcggc agcaaggatc gcctgcctga
     1681 tttcggcctc tagaggattg gtgatggccg aggtcatgcc ggcccccatc accatcgcca
     1741 gataagccgc gttgatccct gcccgatttg ggagcccgaa gctgatgttc gaggcgccgc
     1801 acgtggtgtt gactcctagc tct
//
LOCUS       DBNHXU010000173         3462 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3030338, whole genome shotgun sequence.
ACCESSION   DBNHXU010000173 DBNHXU010000000
VERSION     DBNHXU010000173.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3030338"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="AC161X_04390"
     CDS             complement(<1..578)
                     /locus_tag="AC161X_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988819.1"
                     /translation="MRRRAGVVGGALLLVLLTPGTALAHGIGGRLDLPVPVTYFIAAA
                     GVVIVISFVALALLWPEPRLQGGPRQEPGRLTVPRRGLLPVLGVIGLLLVIGQLVVAA
                     LSLTTDPTRPSIAPVLIWVVFWLIIPFTGALIGDWYSDLNPWRTIGRTFKIGRAERLW
                     LRDAVGVLPAAALLLSFTWLELVNHESGSATAL"
     gene            complement(578..1012)
                     /locus_tag="AC161X_04395"
     CDS             complement(578..1012)
                     /locus_tag="AC161X_04395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR4988820.1"
                     /translation="MRRLLLATMVAALVIACGDTATTTTTAVPAATTTTTAQPVTTTT
                     EEGATSTVPNTTTTIREADVTVTDGVVDGPDTFEYALGDQVDIVVLTDVADEIHVHGY
                     DLMFDAGPGVPVEISFEADAQGIFEVELEGSELKLFEIQVTP"
     gene            complement(1132..2031)
                     /locus_tag="AC161X_04400"
     CDS             complement(1132..2031)
                     /locus_tag="AC161X_04400"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4988821.1"
                     /translation="MKLLRRFFFGFLLWRLFGPVLSPRFRSPQEHPWRHVGHTVFVGD
                     QEFLVRDLGPDDAPPLLLIHGLAGSSLAEWYRIAPALAEKYRVIMVDHRSHGLAPLAR
                     DRFDVDEIADDMAGVLDALNIGIVSLVGYSMGGTIAQSFAHRYPARVQRLVLIATFTH
                     HPEPMRTFRVLGVVVARAWERLTGLGTAEVRTGYLLATGAVEQRHARWLWEETHRRDT
                     DAGAQASLALLRFDSRPWIGRIEAETLVMIPTQDQLVPPAWQYQLGAALRDPKLVELV
                     GVRHEAPWTHAERLVDEIALFVG"
     gene            complement(2028..2453)
                     /locus_tag="AC161X_04405"
     CDS             complement(2028..2453)
                     /locus_tag="AC161X_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011682741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HIR4988822.1"
                     /translation="MDDRQRLEAIADALSIPRAERHIFLCADQTTPKCAPREETNLLW
                     AHLKSRLKTEGLTSAPPPWSGKPEAEATPAEPGEGRVLRNKVDCLRVCEQGAICVVYP
                     EGTWYAGLDIAKLDRIIDEHLIGGTPVEELVFAIGGLGQ"
     gene            2545..3021
                     /gene="greA"
                     /locus_tag="AC161X_04410"
     CDS             2545..3021
                     /gene="greA"
                     /locus_tag="AC161X_04410"
                     /inference="COORDINATES: protein motif:HMM:NF013440.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HIR4988823.1"
                     /translation="MADQPTFLTPAARDKILDELETLKTDGRKEIEERIAEARSHGDI
                     RENADYDAAKNDQGMMEARIRKLEHLLNTAEVRAVEDTGKVEVGSVVTVVDRDGDEFE
                     CFVAPQENKVAGMMLASPTSPLGGALLGASVGDEVSYEAPGGEFKVVIKAVRPFEG"
BASE COUNT          697 a         1067 c         1119 g          579 t
ORIGIN      
        1 agggccgtcg ccgatcccga ctcatggttg accaactcca gccaggtgaa cgacaacagg
       61 agtgcggcgg cagggagaac gccaacggca tcgcgcagcc acagccgctc ggcgcgcccg
      121 atcttgaagg ttcgcccgat ggtgcgccac gggttgagat cggagtacca atcaccgatc
      181 agcgctccgg tgaagggaat gatcagccag aagacgaccc agatcagcac cggagcaatc
      241 gaggggcggg tgggatcggt tgtcaggcta agggcggcta ccaccagctg cccgataacc
      301 agtagcaagc cgatgacacc caacaccggg agcaaccccc gtcgaggaac tgtgagccgt
      361 cctggttcct ggcgggggcc tccttgaagc ctgggctcgg gccagagcaa tgccagggcc
      421 acaaagctga tcacgatgac gacacctgcg gccgcgatga aataggtgac gggaaccggc
      481 aggtcgagac gacccccaat gccatgggcc agggccgtcc ccggggtgag aagaaccagg
      541 agcagagccc cgccgacgac gccggcgcga cgtctcatca gggggtgact tggatctcga
      601 acagcttgag ttcactgcct tcgagctcga cttcgaagat gccctgggca tcggcctcga
      661 atgagatctc gaccggaacc ccgggccctg cgtcgaacat gaggtcgtag ccgtgaacgt
      721 gaatctcgtc ggcgacatcg gtgagcacca cgatgtcgac ctggtcaccg agcgcatact
      781 caaaggtgtc cggcccatcg acgacaccgt cggtcaccgt gacatctgcc tcgcgaatgg
      841 ttgtcgtggt gttcgggaca gtgctggtgg ccccctcctc ggtagtggtc gtcaccggct
      901 gagccgtggt tgtggtggta gcggcaggaa cggcagttgt ggtcgtggtt gcggtgtcgc
      961 cgcaggcgat gaccagggcc gcgaccatcg tcgccaggag tagacgtcgc atccgcaaaa
     1021 ggtagatcga tccgggcgga atgccgattc gaccaatgag gtcccaagtc gcaggtcaca
     1081 ggtctcaggc cagaattctc tgcctgagac ttaggacctg tgacttgtga cctaacccac
     1141 gaaaagggct atctcgtcga cgaggcgctc ggcgtgagtc cacggtgcct cgtgacggac
     1201 accaaccaac tccacgagct tcgggtcgcg aagcgcagcc cccaattggt actgccaggc
     1261 cgggggcacc agttgatcct gagtgggaat catcaccagt gtttcggcct cgatccggcc
     1321 gatccagggg cgggagtcaa agcgaagcaa ggccaggctg gcctgtgctc cggcgtcggt
     1381 gtcccggcga tgggtctcct cccaaagcca gcgggcatgc cgttgctcaa ccgccccggt
     1441 cgccaatagg tagccggtcc gcacctcggc agtgccaagg ccggtgaggc gctcccatgc
     1501 cctggcgacc acgacaccga gtacccggaa ggttcgcatc ggctcgggat gatgggtgaa
     1561 ggtggcgatg aggacaagcc gctgcacccg ggccggatag cgatgggcga acgactgagc
     1621 gatcgtccct cccatcgagt aaccgaccag cgagacgatg ccgatgttca acgcatcgag
     1681 cacaccggcc atgtcgtcgg caatctcgtc gacgtcgaac cgatcccgcg ccagaggggc
     1741 cagcccatga cttcgatgat cgaccatgat cacccggtac ttctccgcca gcgccggtgc
     1801 gatccgatac cactcggcga gcgaggagcc ggcaagacca tggatgagaa gcagcggtgg
     1861 cgcgtcgtcc gggccgagat ctctcaccaa gaactcctgg tcgcctacga acacggtgtg
     1921 acccacgtgt cgccatggat gctcctgggg agaccggaag cgtgggctca agaccggtcc
     1981 gaacaaccgc cagagcagga acccgaagaa gaagcggcga agcaacttca ttgaccgagt
     2041 cctcctatcg cgaacaccaa ttcctcgacc ggcgtgccac caatcaggtg ctcgtcgatg
     2101 atccggtcga gcttggcgat atcgagcccc gcgtaccagg tgccctcggg gtatacgacg
     2161 cagatcgcgc cttgttcaca gaccctgagg caatcgacct tgttcctgag gacccgaccc
     2221 tcacccggct cagcgggggt ggcttccgcc tccggcttcc cggaccaggg aggtggggcc
     2281 gaagtcaggc cctccgtctt gagacgactc ttgagatgag cccacaacag gttggtctcc
     2341 tctcgaggcg cacacttggg agtggtctgg tccgcacaca gaaagatgtg gcgctcggcg
     2401 cgaggaatcg agagagcatc ggcgatggcc tcgagacgtt gtcgatcgtc cacatcaaga
     2461 agttagatca cggcggcacc tttgtcggat gcgaccggtc tccggtcttg atttgtcgcg
     2521 tgcggggtta gcctctctgc ctcgatggct gaccaaccaa cctttctcac acctgctgcc
     2581 cgcgacaaga tcctggatga gctggaaacg ctcaagacgg atggccgcaa ggagatagaa
     2641 gagcggattg ccgaggcgcg ctcacacggt gatattcgtg agaacgccga ctatgacgcg
     2701 gccaagaacg accagggcat gatggaagcc cggattcgca agttggagca tctcctcaac
     2761 accgccgaag tccgggccgt ggaggacacc ggaaaggtcg aagtcgggag cgttgtcacc
     2821 gtcgtcgatc gtgatggtga cgagttcgaa tgtttcgtcg cccctcagga gaacaaggtg
     2881 gcgggcatga tgctggcctc tcccaccagc ccgctcggag gagctctcct cggggcatcg
     2941 gtgggtgacg aggtctccta cgaagcgccc ggcggggaat tcaaggtcgt catcaaggcg
     3001 gtgcggccct tcgaaggctg actcgacagg aggccatctc gcctatggcc tggcacccag
     3061 tggcaactag catgatgggc aagcgcgtct atgacgcgtc attctgatct gtctacaagg
     3121 aggttgcggt ccgtgaccgc tgacagtccg agacctatgg gtgctgtctc cctccgaagt
     3181 cgggccttcg acatcacggc ctgatcttct cggtgggggt ggcagcgccc ctgttcgccg
     3241 aggagaattt gatgacgatc cgctggtttc aagcgcgaga gagcgcccgc ggttgcgtgg
     3301 agagccgacc atgaccgagc cccagaccag cgcgtctcct ggatgggtga ggcgggctgt
     3361 gcgcaccccg cgcgacctgg ccagatggct gcgatcactt gccatcaaag agcctcgacg
     3421 ggccgaggag tacatcgccg accacgccac cgattggtcg ac
//
LOCUS       DBNHXU010000174         4041 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2633646, whole genome shotgun sequence.
ACCESSION   DBNHXU010000174 DBNHXU010000000
VERSION     DBNHXU010000174.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4041)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4041)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4041
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2633646"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="AC161X_04415"
     CDS             complement(<1..197)
                     /locus_tag="AC161X_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988824.1"
                     /translation="MRRRPNPWIAIPALVTGLLAGAIGWVVTDVSCETSCAGWATLVS
                     VVSFLVVTIGVVLILALVYRSL"
     gene            complement(194..937)
                     /locus_tag="AC161X_04420"
     CDS             complement(194..937)
                     /locus_tag="AC161X_04420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988825.1"
                     /translation="MKVPWASASPLVVAVLWTIALTVDPGPLAPWSVFLVGFGLMTTA
                     TVAVVGMIIVAGRWARRTGLASILGGLLIAVARPVDTLWVAAVVVSVLAWVVLFLPSV
                     NERIRKLPSATGPPPRAVLVPLVLVATPFTVGLAAWDRPTVATVIVGLSAPLAALWYS
                     RVLPGGLFAVRIIWPLLALVLAPLQPLPAGVVSALTALVVVALAWGRQVEIAFHPRRE
                     KGQVFPIPPELAPKEILDAAQIDERGRPR"
     gene            complement(963..1637)
                     /locus_tag="AC161X_04425"
     CDS             complement(963..1637)
                     /locus_tag="AC161X_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR4988826.1"
                     /translation="MAALIHLVRHAEVDNPEHLVYSDLPGFGLSERGLEQARRVGRYL
                     GARPVVAIWSSPLERSLRTAEEIAGRTGVPVKVDPGLTEWRVTNRWRGHSWSDIPDAF
                     PGELEAYLEHPETLTFAEESLNDLAERMAATARRLDTDHPHGDVVIVSHQDPIQAGRL
                     RLIGSALTGLHDEKPGHGAVVTLRPGEVWREETMWEPGDSTRIADHQGLRVVSQHEES
                     VDPTSA"
     gene            1687..2871
                     /gene="dinB"
                     /locus_tag="AC161X_04430"
     CDS             1687..2871
                     /gene="dinB"
                     /locus_tag="AC161X_04430"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014375995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR4988827.1"
                     /translation="MWTEPILHVDMDAFFVEVERLGDASLRSKPVAVGGGGKRGVIAS
                     ASYEARKAGVHSAQPTAIALRMCPNLLVVAPDHSRYGAVSSQVFEIFRSFTPYVEGLS
                     LDEAFLDVLGLRFHFESPVHVGQEIRSRIKSELGLPASVGVASTKFMAKLASEAAKPD
                     GLLHIPSSDQIEFLHGLPVSSLWGVGPATLAGLQRLGVVTVGDLAELPEATVVSALGP
                     ANGRHLLALARGIDERQVEPDIEAKSISVEETYETDLEGKDVVQAALLAHAQSLSGRL
                     RRSGLAARTVHLKVRYQDFTTITRSLTSEAAVDSPHDLYRIGLGLLDQVDLDRPVRLL
                     GLGGSTLEDGSQPRQLTLDASEDWDRLAEAVSGVRERYGDRAVEPARLLEQRPLQRGI
                     DP"
     gene            2938..3234
                     /locus_tag="AC161X_04435"
     CDS             2938..3234
                     /locus_tag="AC161X_04435"
                     /inference="COORDINATES: protein motif:HMM:NF022684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3040 domain-containing protein"
                     /protein_id="HIR4988828.1"
                     /translation="MPLDDHEQKILAEIERQFYEEDPELARAVRDITRPSRIGARLAV
                     LGVIAGLAIVIGFFTTRTVIAILGFVLLVVSATALVTGIRARGWQVPEPEPDDD"
     gene            3393..3893
                     /locus_tag="AC161X_04440"
     CDS             3393..3893
                     /locus_tag="AC161X_04440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4988829.1"
                     /translation="MEQSFTAEQACRLTNCTHHQLRYWDRVGLVSPSVQRTGGRPGVR
                     RLYSFRDLVALRVVRSLLDNGMSLQRVRRAWDYLRREGEMEDHLADIKLVTDGQTIFH
                     VGSDEGELMDALRQGQLAFFVAIDEIAREVEEDVTRFELDRNRFLDMLRRVEDDVADE
                     QRAASS"
BASE COUNT          738 a         1286 c         1342 g          675 t
ORIGIN      
        1 aggctgcgat agaccaatgc caggatcagc accacgccga tggtcaccac caggaacgag
       61 accaccgaca ccagggttgc ccaacccgca cacgacgtct cacagctgac gtcggtcacc
      121 acccaaccga ttgcgcctgc caggaggccg gtgacgagtg cggggatcgc gatccagggg
      181 ttcgggcgtc gtctcatcgg ggccggcccc gctcgtcgat ctgggcggcg tcgaggatct
      241 cctttggagc cagctcaggc ggtatcggga acacctggcc cttctccctc cgtgggtgaa
      301 aggcgatctc gacctgccgg ccccaggcca gggccactac cacaagggcg gtgagagccg
      361 agaccacgcc agcgggaagg ggctgcagcg gagccaggac gagggcgagg agtggccaga
      421 tgatcctgac tgcaaagaga ccgccgggca ggactctcga gtaccagagc gccgccagcg
      481 gtgcgctgag cccgacgatg acggtcgcca cggtcgggcg atcccaggca gccagcccga
      541 cggtgaatgg cgtcgccacc aacacgagcg gcacgaggac ggcgcgcggg ggagggccag
      601 tggctgaagg gagcttgcgg atccgctcat tgaccgatgg caggaatagg accacccatg
      661 cgaggaccga gacgacgact gcagccaccc acagggtgtc gaccggcctg gccacggcga
      721 tcagcaggcc gcccaggatc gaggccaacc cggtgcgacg tgcccaccgc ccagccacga
      781 tgatcatccc aaccaccgcc acggttgcgg tggtcatcaa accgaagcca acgaggaaca
      841 ccgaccacgg ggcgaggggt ccgggatcga ccgtcagtgc gatggtccat agaacggcga
      901 caaccagagg ggaggcggag gcccagggaa ccttcaccca ggaagtctcg cagcggaaag
      961 ggtcaagccg aagtcgggtc caccgactcc tcatgttgac tgacgaccct cagcccctga
     1021 tggtcggcga tcctggtcga gtcaccaggc tcccacatcg tttcctctcg ccagacttcg
     1081 ccgggtcgca gggtcaccac cgctccgtgc cctggcttct catcatggag cccggtcagg
     1141 gcggagccga tgaggcgcag ccgaccggcc tggatcgggt cctgatgcga aacgatcacg
     1201 acgtccccat gggggtggtc ggtgtcgagg cgacgagcgg tcgccgccat gcgctcggcc
     1261 aggtcgttga gggactcctc ggcgaaagta agggtctcgg gatgctcgag gtaggcctcc
     1321 aactcgccgg ggaaagcatc gggtatgtcg ctccaactat ggccccgcca tcggttggtc
     1381 acccgccact cggtcaaacc agggtcgacc ttcaccggga ctccggtgcg gcccgcgatc
     1441 tcctcggcgg tcctcagcga acgctccagg ggagaggacc agatggcgac cacgggacgt
     1501 gcgccgagat agcgaccgac ccgccgtgct tgctcgaggc cccgttcgga gagaccgaac
     1561 cccgggaggt cggaatagac gaggtgctcg gggttgtcga cctcggcgtg acgtacgaga
     1621 tggatcaggg cggccattga cctcagtttg gcggttcctg tcggttgtca gaggtaactt
     1681 ggccaggtgt ggaccgaacc gatcctccac gtcgacatgg atgccttctt tgtcgaggtg
     1741 gagcggctgg gcgacgcatc gttgagatcc aagccggtgg cagtcggggg cggtggcaag
     1801 cggggcgtga tcgccagcgc gtcctatgaa gccaggaagg ccggtgtcca ctccgcccaa
     1861 cccacggcca tcgccctccg catgtgccca aacctgctcg tggtggctcc cgatcacagt
     1921 cggtacgggg cggtgtcctc acaggtattc gagatattca gatccttcac cccctatgtc
     1981 gaagggctga gtctcgacga ggctttcctc gatgtcctcg gattgcggtt tcacttcgag
     2041 tccccggtcc atgtcggcca ggagatcagg tcccgaatca agtccgaact gggacttccc
     2101 gcatcagtcg gcgttgcttc caccaagttc atggcgaagc tggcctccga ggcggccaag
     2161 cccgatggcc tcctccacat tcccagcagc gaccagatcg agtttctcca cggccttccg
     2221 gtcagctcgc tgtggggtgt tggcccggcg acgctcgccg gtctgcagcg attgggcgtg
     2281 gtgaccgttg gcgaccttgc cgagctcccg gaagcaacgg tggtctcggc cctgggcccg
     2341 gccaacgggc gtcatctcct ggctttggcc cgggggatcg acgagcgcca ggtcgagccc
     2401 gacatcgagg caaagtcgat ctcggtggag gagacgtacg aaaccgatct cgaaggcaag
     2461 gacgtggtcc aggccgccct cctggcccat gctcagagcc tgtccggccg gcttcggcgc
     2521 agtgggctgg cggcgaggac ggtgcacctc aaggtgcgct atcaggactt cacgacgatc
     2581 actcgaagtc tcacgagcga ggcagcggtc gacagcccgc atgatctcta tcggatcggc
     2641 ctcggtctgc tggaccaggt cgatctcgat cggcccgtga gattgctcgg cctgggtggc
     2701 tcgactctcg aagatggcag ccaaccgagg cagctgactc tggatgcctc cgaggattgg
     2761 gaccgtttgg ccgaagcagt gtccggagtc cgggagcgtt acggcgatcg cgctgtcgag
     2821 ccggcccgac ttttggagca gcgacccctt caaagaggaa tcgacccgtg aaactgtctt
     2881 cgggtcaggt gtattgtttc atgcgggcgt ggatctagct tgaggaacga ggtagcgatg
     2941 cccctcgacg atcacgaaca aaagatcctg gcggagatcg agcgccagtt ctatgaagaa
     3001 gaccctgagc tcgcccgagc ggtccgggac atcacccggc cctcgcggat tggggcccgt
     3061 ctcgccgtgc tcggagtgat cgcaggtctc gccatcgtca tcggattctt caccacgcgc
     3121 accgtgatcg ccatccttgg attcgtactc ctggtcgtgt cggcgaccgc tctggtgacc
     3181 gggatccgcg ctcgcggctg gcaggtgccc gagcccgagc ccgacgacga ctgaaccgct
     3241 ccactccacc ccgcctcctc acgccgcgcc cgttttgtaa cggctaatgt tccggccccc
     3301 ggcggcttcc caaggagggt gggaacgctt ccaaggagga ggactcgacc ccatcatcag
     3361 ggtcgtaggt gagttgttgt aggaagggac gagtggagca gtcattcaca gccgagcagg
     3421 catgccggct gaccaattgc acgcatcatc aacttcgtta ttgggaccgg gtcggattgg
     3481 tcagcccctc cgtccaacga accggcggtc ggccgggagt caggcgcctc tactcatttc
     3541 gcgatctcgt cgcattgcgt gtggtgcgca gcctgctcga caacgggatg tcactgcagc
     3601 gggtcagacg ggcgtgggac tatctgcgtc gggaggggga gatggaggat cacctcgccg
     3661 acataaagct ggtgaccgat ggtcagacca tcttccacgt tgggtctgac gagggtgagc
     3721 tgatggatgc cctccgccag ggccagctgg ccttcttcgt cgccattgac gagatcgcca
     3781 gagaggtcga ggaggacgtg accaggttcg agctggatcg caaccgcttc ctcgacatgc
     3841 tgcgtcgggt cgaagacgac gtcgccgacg agcagcgagc ggccagcagc taggtcaggg
     3901 ctggcggtcg accaacgacc gcagactgaa gaccgctctc gcccgctccg ggccttcgta
     3961 gcgaagtttc agccactgct cgaaatggtc gaggtccgat tgggtggccc gcccattgcg
     4021 tccgccatag acagcagccg a
//
LOCUS       DBNHXU010000175         2494 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3825938, whole genome shotgun sequence.
ACCESSION   DBNHXU010000175 DBNHXU010000000
VERSION     DBNHXU010000175.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2494)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2494
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3825938"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..967)
                     /locus_tag="AC161X_04445"
     CDS             complement(<1..967)
                     /locus_tag="AC161X_04445"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR4988830.1"
                     /translation="MTRTAEVVVVGSGIIGASIAYQLARHGVGNVIALDKGKGPSEGS
                     TGASSSVIRCKYSNPQVVRLAFHGQESYGNWTEFTGLAEPRSGLQQLGVLWMMGHSTS
                     DVEADAAMLVSQGVSARAIGPDEVGSMFPALSDCGARLDFEDPDSHVCVPGDAFLFET
                     DGGHADPVGANQDLIEAARQLGVEVDFNSTVTSVLKENGRVTGVRLADGSEISAGLVI
                     NASGPWCNQLSEMAGADLRWTLSPIRAQIVYTTWPADLGPLPVAGDNSTGIYFRPQSG
                     GQQVLIGSFLQEDEEEVIDDPDDFKKVPDADFIEMKLAAFQHRVPS"
     gene            complement(964..1980)
                     /locus_tag="AC161X_04450"
     CDS             complement(964..1980)
                     /locus_tag="AC161X_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013678282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HIR4988831.1"
                     /translation="MEEERIALFVDFENLAIGAREALNGMEFDFEPIANALAERGRVV
                     ARRAYADWSMFADARQKMTRSHVELIEIPQRMGASRKNAADIKMAVDAIELAFERDYV
                     TTFVICTGDSDFTPLVHKLRELNKKVIGVGVKASTSALLPPACDEFLFYENLEGVEPL
                     RAEGSPRPEPEEEVSDSGTAELESLITTTLAGLQRSVGGEVLASSLKRALLRKDSTFS
                     EADYGFRAFGELLRHLESEGTIELSEGPGRGDPVVQFPSKGTSEQAAFDLLADVVQKL
                     ASKNGPVVLSGLKNQLRKEDANFSEKKFGYGGFLQFCKAAATKGVIDLTWDEGVGDYQ
                     VSVK"
     gene            2071..>2494
                     /locus_tag="AC161X_04455"
     CDS             2071..>2494
                     /locus_tag="AC161X_04455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988832.1"
                     /translation="MTTPSELRVHGVSGTAPRDMLYSDPVLRDLPTPENKYTHVFEEP
                     DRDEGYEVEGFHWGGLTAGDWKTVFWILLAPFALANVAGWTSKSTSKWGVALVRVTAL
                     SLTALLVSQAIIALVLIPYLWFVKEFASLSSDTGNLAAI"
BASE COUNT          465 a          777 c          786 g          466 t
ORIGIN      
        1 gcgaagggac ccgatgctga aaggcggcaa gcttcatctc gatgaagtcg gcatctggca
       61 ccttcttgaa gtcgtcggga tcgtcgatga cctcttcttc atcctcttgc agaaatgagc
      121 cgatcaatac ttgctgtcca ccggactggg gtcggaaata gatgccggtc gaattgtcgc
      181 cggcaaccgg aagcggcccg agatcggccg gccaggtcgt gtagacgatt tgggcccgga
      241 tcggggagag ggtccaccga aggtcggcgc cagccatctc gctgagctgg ttgcaccacg
      301 gcccggaagc gttgattaca agaccggccg agatctcaga accgtcggcc agtcgaaccc
      361 cggtcacccg cccgttttct ttcaggaccg aggtgacagt ggaattgaaa tccacctcga
      421 ctcctagctg gcgcgccgcc tcgatcaggt cctggttggc gcccaccgga tcggcgtggc
      481 cgccatccgt ctcgaagagg aaggcatcgc cgggcacaca gacatgggag tcgggatcct
      541 cgaagtccaa gcgcgcccca caatcggaaa gtgccgggaa catcgagccg acctcatcag
      601 ggccgatggc tcgggcgctg acaccctggg agaccagcat cgcggcatcg gcttcgacgt
      661 ctgaagtcga gtgacccatc atccagagga cgccgagttg ttggagcccg gaacggggct
      721 cggcgagccc ggtgaactcg gtccagttcc cgtaggactc ctggccgtga aatgcgaggc
      781 gaaccacctg agggttggag tacttgcatc tgataaccga cgacgaggct ccggtcgagc
      841 cttcggacgg cccttttccc ttatccaggg caatgacatt gccgacgccg tgacgggcca
      901 gctgataggc gatcgaggcg ccgatgatcc ccgatccgac gaccaccacc tcggcggtgc
      961 gggtcatttg acggagacct ggtagtcgcc gaccccctcg tcccaggtga ggtcgatgac
     1021 tcctttggtg gctgccgcct tgcagaactg gaggaagccg ccgtacccga acttcttttc
     1081 cgagaagttc gcgtcttcct tccgcaactg gttcttgaga cccgaaagga caactggtcc
     1141 gttcttcgag gccagcttct ggacgacatc ggccaacagg tcgaaggcag cctgctcgct
     1201 ggtgcccttc gatgggaact gaacgaccgg atccccccga cccggtcctt ccgagagctc
     1261 gatggtgccc tcggactcga ggtggcggag cagttctcca aaggccctga acccgtagtc
     1321 ggcctcactg aaggtcgagt ccttgcggag cagcgcccgc ttcagagagg aggcgagcac
     1381 ttccccaccg accgagcgtt gcaaaccggc gagggtggtc gtgatcagcg actcgagctc
     1441 tgcggtgccg ctatccgaaa cctcctcctc cggttcggga cgggggcttc cctcggctcg
     1501 gagcggttcg acaccttcga gattctcgta gaacaagaat tcgtcacagg ccggcggaag
     1561 caaggccgat gtcgaggctt tgaccccgac gccaatcacc ttcttgttca actcgcggag
     1621 cttgtgaacc agcggggtga agtcggagtc tccggtgcag atcacgaaag tggtgacgta
     1681 atcgcgctcg aaagcgagct cgatggcgtc gaccgccatc ttgatatcgg cggcgttctt
     1741 gcgcgaggcc cccatgcgct gggggatctc gatcagctcg acatggcttc gggtcatctt
     1801 ctggcgggcg tcggcgaaca tcgaccagtc ggcatatgcc cggcgggcga cgacccggcc
     1861 cctctcggca agggcgttgg cgatgggctc gaagtcgaac tccatcccgt tcagggcctc
     1921 acgggccccg atcgccaggt tctcgaagtc gacgaacaag gcgatgcgtt cttcttccat
     1981 ggggatgaat gtagtgccga aggggtggaa ttccccatgt cggacctctg agcggccgga
     2041 gtaggttgcc cacgctgcct agggagaaga ttgacgacgc ccagcgaact tcgggtgcat
     2101 ggggtatcgg ggaccgcacc ccgggacatg ctctacagcg acccggtgct gcgcgaccta
     2161 ccgaccccgg agaacaagta cacccatgtc tttgaggagc ctgaccggga tgaaggctac
     2221 gaagtcgaag ggttccattg gggtggcctc accgccgggg actggaagac cgtgttctgg
     2281 atcctgctcg ccccgttcgc ccttgccaac gtcgccggat ggacctcgaa gagcactagc
     2341 aaatggggtg tcgccctggt ccgggtcacc gccctgtcgc tcacggcatt gctcgtttcg
     2401 caggcgatca tcgccttggt ccttatcccc tacctctggt ttgtcaaaga gttcgcctcg
     2461 ctgagctcgg acaccggcaa cctggcggcg atcg
//
LOCUS       DBNHXU010000176         7923 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4817635, whole genome shotgun sequence.
ACCESSION   DBNHXU010000176 DBNHXU010000000
VERSION     DBNHXU010000176.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7923)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7923)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7923
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4817635"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="AC161X_04460"
     CDS             <1..320
                     /locus_tag="AC161X_04460"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR4988833.1"
                     /translation="TTCDRDANWCDVHHLAHWAAGGETNIDNLVLLCRTHHVLVHDGG
                     STIRGKPGQLRFYRPDGTELGSQPPSRPPHRSPVFTVDTERYFPPGGLLPMILNRQRV
                     RAP"
     gene            363..1106
                     /locus_tag="AC161X_04465"
     CDS             363..1106
                     /locus_tag="AC161X_04465"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR4988834.1"
                     /translation="MCYDDDSSPPIEVIDGGVGGHGDLVLTAADGNQLAAFSATSANS
                     KGPGVVILPDVRGLHRFYEQLALRFAENGYDSIAIDYFGRTAGVGKRRDAFPYADHVP
                     LTTIEGVTADTAAAVAQLRSTDPGRVIFTVGFCFGGSSSWHQAAAGHGLSGAIGFYGN
                     PKRPPVGPIERVHDFECPVLGLMAGDDPGIPVEVVDEFRSALDSAGKENEIIVYPGAP
                     HSFFDRKQEEFVEASADAWRRVLDFIGSK"
     gene            1103..3724
                     /locus_tag="AC161X_04470"
     CDS             1103..3724
                     /locus_tag="AC161X_04470"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4988835.1"
                     /translation="MTPSAPRALPGGTVTFLFLDIEKSTEHLEQLGTEYPHLLETYWD
                     LTERAVADHAGVIFGSEGDGLFIAFPEVGAAVASAIDAQVSYATAPWPHGSKVLVRMG
                     IHTGTPTLIGDDYTGLDVHRAARIMAAAWGGQILLSDVTFSLLSRPGLTCRELGWFDM
                     KGLTRPERIYQLEAPPLASEFPPVRARNREVDLPAYLTEFIGREDDISALLGMLEGGA
                     RMITLTGPGGIGKSRIAAAAASRLGSTYPDGVTFVDLSNETDPDRVPGAIAEKLGLPP
                     EPDHDPLEVLINQLAEMEMLLILDGFERVAAAAVSMATLLSRCPKLRMIVTSRGSLRI
                     GAEREFRVDPLGWAMEGADFDQIAASPAVRLFVDRTRLVRPDFELTPENAPVIRELVE
                     RLDGCPLAIELAAARARLLPPEEILDRLEAVLDLSTSSPDLPTRQRSLRATIEWSHGL
                     LDEADQRLFRRLGVFVDGWALEAAEAVAGESAPDVFLGLETLVSQSMIWVESAGRMAM
                     GTAMREFALEQLADSGDEEETRRRHAEHYDAVVVASEPLLQGRKQRETVAKLSRDWRN
                     LRSAADWAMKNNRIQMAGSLYVRTWIVAWQGDNWNNVKADTARLMAFSDRLDEPLRAR
                     ALFVAAGTFMEVGEPEAAISYGRPAMDLAARIGDRETEAWARLMIAGSILFEDSFSQE
                     ARTHIATAVELASTLDDPFVLAYATSFHAAMSTVDGDIESALQGHARVLSIALEMDNV
                     SLIVQSYCQAALTHLAAGDRVRAREVLEAAAEYVDRLRSLEGLALFLDSVSWLAFTEE
                     DPVRAMTALGAANATRARVGLVRWATVAILLESTGLAAEAETPELAEARRAGGEMSPQ
                     DAISFALHPHHELATAG"
     gene            complement(3737..5092)
                     /locus_tag="AC161X_04475"
     CDS             complement(3737..5092)
                     /locus_tag="AC161X_04475"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014431989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR4988836.1"
                     /translation="MTDSADAVVIGAGVAGVSAAYHLAVAQGAKRVKIVDPRPPLTLT
                     SDKSAECYRNWWPNSPMVELMNRSIDLLEKVATESGNVIGMNRRGYLFITADEARLHS
                     MIEEAHHTSTLGAGTVRIHPGPIPYRHSPEEGYMGAPEGADILIGAGTIREHFPFITD
                     KAVGAVHVRRAGWLSAQQLGAWMLERARQDGATFVQDEVTEIDVDGGSVTGVGLASGD
                     RIATKVVVNAAGPMSRSVAAMVGVALPVLAEVHIKVAYREHLGVIPRHAPMFIWSDHQ
                     TVDWTDEERAELQAQGRHDLLGQMPIFCHGRPEGGVESPYLLALWEYHNEVREDPVWP
                     LLDDPLYPEVVMRGLTTMVPGLAPYLDGLPHSVVDGGYYLKTRENRPLVGPAGPEGFF
                     LMAGMSGFGVMVSMGAGDLVARHISGDELPGYSPSFLLSRYDDPVYLESIADAGRSGQ
                     L"
     gene            complement(5093..7036)
                     /gene="tkt"
                     /locus_tag="AC161X_04480"
     CDS             complement(5093..7036)
                     /gene="tkt"
                     /locus_tag="AC161X_04480"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020374456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HIR4988837.1"
                     /translation="MESSPVEQLAVNTIKALAMDAVQKADSGHPGMPMGMADIAVVLW
                     GRYLNVDPDDPEWPDRDRFVLSNGHGSMLLYSMLHLSGFPLTLDDLRNFRQWGSHTAG
                     HPEIDHRLGIEMTTGPLGQGFATGVGMAIAEAHLRARLGADLVDHRTFGFVSDGDLME
                     GVSSEAASLAGHFGLGRLIYLYDNNRISIDGSTDITFSEDVVARFDSMGWQTQDIDGH
                     DREAVAAAIESALEDDRPSLISCHTHIAQGAPHAQDTAGAHGSPLGEEEIKLTKEAMG
                     FPIDPPFHVEDGVYEFFSAAMNRGRDARRAWTERFESANTEQRSLWSALHDPTPVKLN
                     GPGFEVGGSLATRASSSMLFGEMAEKAPGFIGGAADLAGSTKTVIEKGRLFSRDDRSA
                     RDIPFGIREHAMGAAVNGMAVHGGLKPYSATFFVFSDYQRPAVRLSAIMRAPSIWVYT
                     HDSIFLGEDGPTHQPIEHLASLRAMPNLWVIRPADANETLQAWEVALNRHDGPVALVL
                     TRQNLPTLDPVPDGVNRGGYAVREGSAVTMIATGSEVSAALGAAEALAGEGIEARVVS
                     LPCWELFFEQEKSYQDEVLGTAPRVSIEAAATFGWERLVGDEGLSIGIDRFGASAPDH
                     VLAKEFGFTPDEIAGRVRTFLGG"
     gene            complement(7082..7834)
                     /locus_tag="AC161X_04485"
     CDS             complement(7082..7834)
                     /locus_tag="AC161X_04485"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: protein motif:HMM:NF013222.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HIR4988838.1"
                     /translation="MNIEQQLEGLAGPLPEGITEGVALGTGIADGYDYYDSPVGEVVV
                     TFNPNGVSSLGLASDDFEGYFNHRHGRPLIRAEAPSAWSGRIPSAIEAGTPGTLPVDL
                     RSVTDFQARILRIATTIPKGEVRPYGWLAREAARPGAARAVGSTMARNPIPLIIPCHR
                     VVRSDGTIGAYSLGGPHVKWELLTHEGADPVHLEDLAEHQVRVQGNKSTHIFCNPTCR
                     AIRRSKPENVVPFKSGSAAEASGYRACELCRP"
BASE COUNT         1383 a         2516 c         2556 g         1468 t
ORIGIN      
        1 tcacgacatg tgaccgtgac gccaactggt gtgatgttca ccatctcgcc cactgggccg
       61 ccggtggcga gaccaacatc gacaacttgg tgttgctttg tcgcacccat catgttctcg
      121 tccacgatgg tggttcgact atcagaggca agcccggtca gctccggttc taccgtcctg
      181 atggcaccga gttgggaagt cagcccccgt cacgaccgcc acatcgcagt ccggtgttca
      241 ccgtggacac cgagcgctac tttccgccgg gtggcctgct cccgatgatc ctcaatcgcc
      301 agcgagtcag ggctccctga cccggcttgg tgcctgccgt catggggtaa gtttggccct
      361 ccatgtgtta cgacgacgac tccagccctc cgatcgaagt catcgatgga ggggtaggcg
      421 gacatggaga cctggtgctg accgcggccg atggcaatca gctcgccgca ttcagtgcca
      481 ccagcgctaa cagcaaagga ccgggggtag tgatcctccc cgatgttcga ggcctacatc
      541 ggttctacga gcagctggcg ttgaggttcg cagagaatgg ctatgactcc atcgccatcg
      601 actatttcgg gcgcaccgcc ggcgtcggca agcggcgtga tgcgtttcct tacgctgatc
      661 acgttcctct caccaccatc gagggggtga cagctgacac ggctgccgct gttgcccagc
      721 tgcgttcgac ggaccccggg cgcgtcatct tcaccgtggg cttttgcttc ggtgggagta
      781 gctcgtggca tcaggcagct gccggccacg gcctctcggg agcgatcggc ttctatggca
      841 atcccaagcg ccctccggtc ggccccatcg agcgggtcca tgatttcgag tgccccgttc
      901 tcggcctgat ggccggggat gaccccggga taccggtcga agtcgtagat gagtttcgat
      961 cggccctcga tagcgcagga aaagagaacg agatcatcgt ctatccgggt gctccccact
     1021 ccttcttcga tcggaagcag gaggagttcg ttgaggcctc agccgacgcc tggcgtcggg
     1081 tactcgactt catcggatcc aaatgacccc gtcagctcca cgggcccttc cggggggcac
     1141 tgtcaccttc ctcttcctag acatcgagaa gtccaccgag catctcgagc aactgggaac
     1201 cgagtacccg catctcctgg agacctattg ggatctcact gagagagccg tcgctgacca
     1261 tgcaggggtc atcttcggct cggagggtga cggcctcttc atagcgttcc ctgaggttgg
     1321 tgccgcggtt gcgagtgcga tcgatgcgca agtttcctat gccacggcgc cttggccaca
     1381 cggctcaaaa gtgttggttc gcatggggat ccacaccggc accccgactc tcattggtga
     1441 cgactacacg ggcctcgatg tccatagggc cgctcgcatc atggctgcgg catggggcgg
     1501 gcagatcttg ctctcggacg tgaccttttc ccttctgagc cgtcccgggc tgacctgccg
     1561 ggagttgggc tggtttgaca tgaagggcct gacccgaccg gagcggatct atcaactcga
     1621 ggcccctccg ctcgcatcgg aattcccgcc ggtgagagcc aggaatcgtg aggtggacct
     1681 tcccgcctac ctgaccgagt tcatcgggcg tgaagacgac atctcggcgc ttctcggcat
     1741 gctcgaaggc ggtgctcgca tgatcaccct gaccgggccc gggggaatcg ggaagagtcg
     1801 gatcgcggcc gctgcagcat cccgtttggg ttcgacctac cccgacggcg tcacctttgt
     1861 cgatctttcg aatgagaccg accccgaccg ggtgcccggt gctatcgccg agaaactggg
     1921 gctcccaccc gagcccgatc acgacccgtt ggaggtcctc atcaaccagc tggccgagat
     1981 ggagatgctg ctgatcctcg atggtttcga acgggtggcc gccgccgcag tctcgatggc
     2041 gacgcttctc agccggtgcc cgaagcttcg catgatcgtc accagccgcg gctcgcttcg
     2101 catcggcgcc gagcgagagt ttcgcgtcga tcccctggga tgggccatgg aaggggcgga
     2161 cttcgaccag atcgcagcct cccccgcggt ccggctcttc gtcgaccgga cccgtctcgt
     2221 gcgcccggat ttcgagctca ccccagagaa cgcccctgtc atccgggagc tggtggaacg
     2281 actggatggg tgtccccttg ccattgagtt ggccgccgcc agagcgcgcc ttctccctcc
     2341 cgaggagata ctcgaccggc tcgaggcagt actcgacctg tccacgtcgt cgccggatct
     2401 gccgactcgg cagcgatcgc tccgggccac gatcgaatgg agtcacggtc tgctcgacga
     2461 agccgaccaa cggctcttca ggcgtctcgg ggtcttcgtg gacggatggg ccctggaggc
     2521 ggccgaggcg gtcgctggtg aaagtgcacc agacgtgttc ttggggttgg agacgttggt
     2581 cagtcagagc atgatttggg ttgagtccgc gggtcgaatg gcgatgggga ccgccatgcg
     2641 cgagttcgct ctggagcaac tagcggactc cggagacgag gaagagaccc ggcggcgcca
     2701 tgccgagcat tacgacgcgg tggtcgttgc cagtgagccg ctgcttcaag gccggaagca
     2761 aagagagacg gtggccaagc tgtcacgtga ttggcgcaat ctgcggagcg ccgccgattg
     2821 ggcgatgaag aacaaccgga tccagatggc cggctccctc tacgttcgga cctggatcgt
     2881 ggcctggcag ggcgacaact ggaacaacgt caaagctgac accgccaggt tgatggcatt
     2941 cagtgaccgg ctcgacgaac cgctccgagc tcgcgcgttg tttgttgccg cgggcacctt
     3001 catggaggtg ggcgagcctg aggccgcaat ttcctatggg cgtcccgcca tggacctggc
     3061 ggcccggatc ggcgacaggg agaccgaggc atgggcccgt ctcatgattg ctggctccat
     3121 cctgttcgaa gacagcttca gccaggaggc ccggacccac atcgccacgg cggtggagct
     3181 tgccagcacc ctggacgacc cgttcgtgct ggcatatgcg accagcttcc atgcggcgat
     3241 gtcgaccgtc gacggtgata tcgagtccgc tctccagggc catgccagag tcctctcgat
     3301 cgcactcgag atggacaatg tgtcgttgat cgtccagtcc tactgccagg cggcccttac
     3361 ccatctcgct gccggggatc gggttcgggc acgagaggtt ctcgaggcgg ctgcggaata
     3421 tgtcgatcgg ctccgaagcc tggagggcct cgcgctgttc ctcgactcgg tctcatggct
     3481 ggccttcacc gaagaggatc cggtgcgggc gatgacagcg ttaggagccg cgaacgcgac
     3541 cagagcccgg gtgggactgg tccgctgggc gacggtcgcc atcctgctcg aatcgacagg
     3601 gttggcggca gaggcggaga caccggagtt agccgaggcc cgacgcgccg gcggagagat
     3661 gtcgccgcag gacgcgatca gcttcgccct tcatccccac cacgagttgg cgaccgcagg
     3721 ctgaccagga tcgcggttag agctgacccg atcgaccggc gtctgcgatt gattcgaggt
     3781 agaccgggtc gtcgtatcta ctgagcaaga acgacgggga gtaccccggc agctcgtcgc
     3841 cagagatgtg acgggcaacg agatctccgg cccccatcga gaccatgact ccgaatcccg
     3901 acattccggc catcaggaag aatccctctg gcccggccgg cccgaccaga gggcggttct
     3961 cccgggtctt gaggtaatag ccaccatcga ccaccgaatg ggggagcccg tcgaggtagg
     4021 gcgccaatcc gggaaccatc gtggtcaggc cccgcatcac gacctcggga tagagggggt
     4081 catccagcaa cggccaaact ggatcctccc ggacttcgtt gtggtattcc cagagggcca
     4141 ggaggtaagg agactcgacg ccgccctcgg ggcgcccatg gcagaagatc ggcatctggc
     4201 cgagcagatc gtggcggccc tgggcctgca gttccgccct ttcctcatcg gtccagtcga
     4261 ccgtctgatg gtcactccaa atgaacatcg gggcgtgacg tgggatcact ccaaggtgct
     4321 ctcggtaggc gaccttgatg tggacctcgg caagaaccgg gagggcgacc ccaaccattg
     4381 cggccaccga tcgcgacatc ggccccgccg cgttgaccac gactttggtt gcgatacggt
     4441 cgccggaggc caggcccacc ccagtcaccg agccgccgtc cacatctatt tcggtgacct
     4501 cgtcctggac gaaggtcgct ccgtcctggc gtgcgcgttc caacatccat gctccgagtt
     4561 gctgggcgct cagccacccg gcgcgtcgaa catgtaccgc cccaaccgcc ttgtcagtga
     4621 tgaacgggaa gtgctctcgg atggtgccgg cgccgatgag gatgtcggcg ccctcgggtg
     4681 cgcccatgta accctcctcc ggtgaatgtc gataggggat cggtccgggg tgaatcctga
     4741 ccgtgccggc accgagggtc gatgtgtgat gggcctcttc gatcatgctg tggagacggg
     4801 cttcgtctgc ggtgatgaac agatagccgc gacggttcat cccgatcacg ttccccgact
     4861 cggtcgccac cttctcgagc aagtcgatgg agcgattcat caattcgacc atgggggaat
     4921 tcggccacca attgcggtaa cactcggctg acttgtccga tgtgagagtc agcggagggc
     4981 gggggtcgac gatcttcact cgcttcgcgc cctgggcgac ggcgaggtga taggcagcgg
     5041 agacaccggc cactccggca ccgatcacca ccgcgtctgc ggaatcggtc atctagccac
     5101 ccagaaacgt gcggacgcga ccggcaatct catctggcgt gaacccgaat tccttggcga
     5161 ggacatggtc cggggccgat gccccgaacc gatcgattcc gatgctcaga ccctcgtcgc
     5221 cgaccaaccg ttcccagccg aaagtggccg ccgcctcaat cgacacccga ggtgcggtgc
     5281 cgagcacctc gtcctgataa ctcttttcct gctcgaagaa cagctcccaa caggggagac
     5341 tcaccacgcg ggcctcgatg ccttctccgg ccagtgcctc ggcagcgccg agggcggcgg
     5401 agacctccga cccggtggcg atcatcgtca ccgcggagcc ctcccgaacg gcgtacccgc
     5461 ctcggttcac accatcgggg accgggtcga gggtagggag gttctgacga gtcaggacca
     5521 gggccaccgg gccatcgtgc cggttgaggg ctacctccca ggcttggagg gtctcgttgg
     5581 catcagcagg gcgtatcacc cagagattcg gcatggcgcg cagcgacgca aggtgctcga
     5641 tcggctggtg ggtcggcccg tcctccccga gaaagatcga atcatgggtg tacacccaga
     5701 tcgagggcgc tctcatgatc gctgacaggc gcacggccgg gcgctggtag tcgctaaaga
     5761 cgaagaaggt ggccgagtag ggcttgaggc cgccgtggac cgccattccg ttgacggccg
     5821 cccccatcgc gtgctcccgg atgccgaagg gaatgtcacg ggccgagcgg tcgtctcgag
     5881 agaagagccg gcccttttcg atgaccgtct tcgttgagcc ggcgaggtcg gcggcgccac
     5941 cgatgaaccc gggggccttc tcagccatct ccccgaacaa catcgatgaa gaggccctgg
     6001 tggcgaggct cccgcccact tcgaagcccg gcccgttgag cttgaccggt gtggggtcat
     6061 ggagcgcgct ccacagactc cgttgctcgg tgttggccga ttcgaagcgt tccgtccaag
     6121 cccgtcgggc gtcacggccc cggttcatgg cagcgctgaa gaactcgtag acaccgtcct
     6181 cgacgtggaa cggagggtcg atcggaaagc ccatggcctc tttggtcaac ttgatctctt
     6241 cctcgccgag gggtgagccg tgggctccgg cagtgtcctg ggcgtggggt gccccctggg
     6301 cgatatgggt gtgacaagag atcagcgacg gacggtcgtc ttcgagagcg gattcgatcg
     6361 cggctgctac cgcttccctg tcatgcccgt cgatgtcctg ggtttgccac cccatggaat
     6421 caaatcgggc gaccacgtct tcactaaagg tgatgtcggt ggacccgtcg attgagatgc
     6481 gattgttgtc gtagaggtag atcagacggc cgaggccaaa atgaccggcc agggacgccg
     6541 cttcgctgct gactccttcc atgaggtcac catcggagac gaagccgaat gtccggtggt
     6601 caacgaggtc cgccccgagg cgcgccctca ggtgggcctc ggcgatggcc atgcctaccc
     6661 ctgtcgcaaa cccctgccca aggggcccgg tagtcatttc gatcccgagc cgatggtcta
     6721 tttcgggatg accggccgtg tggctgcccc attgccggaa gttgcgtaga tcatcgaggg
     6781 tgagcgggaa gccactgagg tggagcatgg aatagagcag catcgagcca tgaccgttgg
     6841 agagcacaaa acggtcgcga tccggccact ccggatcatc cgggtcgacg ttcagatatc
     6901 gcccccacag caccacagcg atatccgcca tgcccatcgg cattccagga tgcccgctgt
     6961 ccgccttctg caccgcgtcc atcgccaggg ctttgattgt gttgacggct agctgctcaa
     7021 ccgggctgga ttccatcggc ggcgagggta cccgtgcaga tcggctgccg gtttcaagtg
     7081 atcagggccg gcacagctcg caggctcgat acccggaggc ctcggcggcg ctccccgact
     7141 tgaaggggac gacattctct ggcttggacc gtctaatcgc tcgacaggtc gggttgcaga
     7201 agatgtgggt cgacttgttg ccttggaccc gtacctggtg ctcggccagg tcctcgagat
     7261 ggaccgggtc ggcgccctcg tgggtgagga gctcccactt cacgtgggga ccgcccaggg
     7321 aataggcacc gatggtgccg tcggagcgaa cgacccggtg gcaggggatg atcaggggga
     7381 tcggattgcg ggccatggtc gaccccacgg cgcgggcagc ccccggacgc gccgcctccc
     7441 gtgccagcca gccatagggc cgaacctcac ccttgggaat cgtggtggcg atccgaagga
     7501 tccgggcctg aaagtcggtt accgacctca ggtcgacagg cagcgtgccc ggtgtgccgg
     7561 cctcgatggc agaggggata cgcccggacc atgccgatgg cgcctctgcc ctgatcagcg
     7621 gtcggccatg ccggtggttg aagtagccct cgaagtcatc cgaagccagg ccaagcgagg
     7681 agacgccgtt tggattgaag gtcaccacaa cctcgccgac gggcgagtcg tagtagtcgt
     7741 agccgtcggc gatgccggtg cccaaagcca ccccttccgt gatgccttca ggcagtggtc
     7801 cagcgaggcc ctcgagttgt tgttcgatat tcatgcttcg tgctccatca tctctcgcaa
     7861 tcggtccaac cctcgatgga tgtctgcctt caccgtgcct tcggggcggc cggtcgtctc
     7921 cga
//
LOCUS       DBNHXU010000177         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1841821, whole genome shotgun sequence.
ACCESSION   DBNHXU010000177 DBNHXU010000000
VERSION     DBNHXU010000177.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1841821"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..391)
                     /locus_tag="AC161X_04490"
     CDS             complement(<1..391)
                     /locus_tag="AC161X_04490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988839.1"
                     /translation="MNAAEALDASGLDITKLADFIRPVRPEQVTLRAASSVMRGVWGR
                     GIQAMTLGNTVYIHPAVLAGPARALGLLVIHELVHVRQWRKRGVFSFLGTYGSDYLKG
                     RRQNLGHRGAYRQIEFEMEARDVASRFD"
     gene            complement(388..2802)
                     /gene="leuS"
                     /locus_tag="AC161X_04495"
     CDS             complement(388..2802)
                     /gene="leuS"
                     /locus_tag="AC161X_04495"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011640998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004823 - leucine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006429 - leucyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HIR4988840.1"
                     /translation="MSYDHKQIEAKWQERWESEKVFRAADDPSRPKFYNLCMYPYPSG
                     ALHQGHVRNYTFGDLLTRYKTMRGYNVLSPMGWDSFGLPAENAAIETGVHPRENTEAQ
                     ITTMTRQIKQLGAMYDWDRELASHHPDYYRWNQWLFLRLWDRGLAYKKEAPVNWCPKD
                     QTVLANEQVVDGACERCGTVVERKNLAQWFFKITDYVERLLADLDLLDEWPDRVRTMQ
                     RNWIGRSEGASFTIPVAGSDASFEVFTTRPDTIFGMTFCVLAPEHPLVADLIAGSEHE
                     GEARAYISQASRASEIERMSEGEKTGVPTGAQAINPVNGKEVPIYIADYVLMGYGTGA
                     IMAVPGQDQRDWEFATRYGLEIIRTVQPPDDWDGEAFVGDGLTVNSEFLDGLDQAAAK
                     LRITDWLEEKGIGKRTVQYRLRDWLISRQRYWGSPIPIVSCPTCGLVPVPEDQLPVLL
                     PDVQDYAPKGQSPLAGVEDWVATTCPSCGGDARRETDTMDTFVDSSWYFFRFTDPHND
                     HIAFEPEKARYWMAVDQYIGGVEHAILHLLYARFITKFLSDIGYSAVSEPFARLFTQG
                     MITLGGSKMSKSKGNVVDPLDLFASHGADALRLYHLFIGPPTDDAAWDTNGVEGTWRF
                     LDRFWRLVTETDRFENASTNRELLAQAHRAVKKVTEDIERFVFNTAIPPLMTLVNSLA
                     EAVRTGIDRETFDEVVELLIKLLAPMAPHISHELWERTGHRTMLAVEPWPEWDEGLVA
                     QATVVMVVQVNGKVRDRVEVPADIAPEEAERIALGLEKVQGWIDGASVQKVVARPPNL
                     INLVVG"
     gene            2851..>3146
                     /locus_tag="AC161X_04500"
     CDS             2851..>3146
                     /locus_tag="AC161X_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020555974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HIR4988841.1"
                     /translation="MSPERVGVVGGGLMGSGIAEVFARSGAQVVIHEIDAGAAEAAQA
                     RVESSLAKAVERGKLEVSLRDQALANIRVSTDLTDQADRQLVIEAATEDEALKK"
BASE COUNT          586 a          997 c          958 g          605 t
ORIGIN      
        1 cgtcgaatct ggaggcaacg tctctcgcct ccatttcgaa ctcgatctgc cgataggcgc
       61 cacgatgacc caggttctga cgacgccctt tcagatagtc actgccgtag gttccgagga
      121 agctgaacac tcctcgctta cgccattgcc gcacatggac gagctcatgg atgaccaata
      181 ggcccaatgc cctcgccggc ccagcaagca cagcggggtg gatatagacc gtgttgccca
      241 atgtcatcgc ttggatgccc ctgccccata cccctcgcat cacagacgag gcggcccgaa
      301 gggtgacctg ctctggtcgc acggggcgga tgaagtccgc gagcttggtg atgtccagcc
      361 ctgaggcgtc gagagcttct gcagcgttca tcccaccacg aggttgatca agttcggcgg
      421 ccttgcaacc accttctgga cactggcgcc gtcgatccag ccctgaacct tttccaaccc
      481 aagagcgatc cgctccgcct cttcaggggc gatgtcggcc gggacttcga ccctgtctcg
      541 aaccttgccg ttcacctgga caaccatgac gactgtcgcc tgagcgacaa ggccctcgtc
      601 ccattcgggc catggttcga ctgccagcat ggttcggtgc ccggtgcgct cccacagctc
      661 gtggctgatg tgaggcgcca tgggggcgag caacttgatc agcagctcca caacctcatc
      721 aaacgtctcc cggtcgatgc ccgtccgcac cgcctcagct agggagttga cgagggtcat
      781 caaggggggg atcgcggtgt tgaagacgaa tcgctcgatg tcctcggtca ccttcttgac
      841 cgcacggtgc gcctgcgcga ggagctctcg gttggtggaa gcgttctcga accgatcggt
      901 ctctgtcacc agccgccaga accggtcgag gaatcgccat gtcccttcga cgccattcgt
      961 atcccaagcc gcgtcgtcgg tgggcgggcc aatgaacagg tgatagaggc ggagcgcgtc
     1021 ggcgccatga gaggcgaata ggtcgagcgg atcgaccacg ttgcccttcg acttcgacat
     1081 cttggagccg cccaacgtga tcatcccctg cgtgaacagc cgtgcaaacg gctcgctcac
     1141 cgcgctgtac ccgatgtcac tcaggaattt ggtgatgaat cgggcataga gcaggtggag
     1201 aatggcgtgc tcgacacccc ctatgtactg gtcgacagcc atccaatacc gggccttctc
     1261 cggctcgaaa gcgatgtgat cgttgtgagg gtcggtgaag cggaagaagt accacgacga
     1321 gtccacgaag gtgtccatcg tgtcggtttc ccgtcgggcg tcgccaccgc aggacgggca
     1381 ggttgtcgcc acccagtctt cgactccggc cagcggcgac tgccctttgg gcgcatagtc
     1441 ctggacgtcg ggcagcagca ccggcagttg atcctccggc acaggtacca ggccacaggt
     1501 ggggcagctg acgatcggta ttgggctccc ccagtaccgc tgtcgagaaa tgagccagtc
     1561 ccggaggcga tactgaaccg tgcgcttccc gatccctttc tcttccagcc agtcggtgat
     1621 gcgcaacttg gcggcggcct ggtcgagacc gtccaggaac tccgagttga cggtcagccc
     1681 gtctccgaca aacgcctcac catcccaatc gtccggaggt tgaacggtgc ggatgatctc
     1741 caacccgtat cgggtggcga actcccagtc acgctgatcc tggccgggga ccgccatgat
     1801 cgccccggtc ccatatccca tcagcacgta gtcggcgata tagatcggga cctctttccc
     1861 attgaccggg ttgatggcct gggccccggt gggcactccc gtcttctcgc cctctgacat
     1921 gcgctcgatc tcggaggcac gagacgcctg agagatgtag gcacgcgcct cgccctcatg
     1981 ctcggaaccg gcgatgaggt cggcgacgag aggatgctct ggggcaagca cgcagaaagt
     2041 cattccgaaa atcgtgtccg gccgagtggt gaacacttcg aatgaagcat cggaaccggc
     2101 gaccgggatg gtgaagctgg cgccctcgga gcgcccgatc caattccgtt gcatggtccg
     2161 cacccgatcg ggccactcgt ccaacaggtc gaggtcggcg aggaggcgtt cgacgtagtc
     2221 ggtgatcttg aagaaccact gcgccaggtt cttgcgctcc accaccgtcc cgcaacgctc
     2281 acacgctccg tcgacaactt gctcgttggc gagaacggtc tggtccttcg ggcaccagtt
     2341 gaccggtgcc tccttcttgt aggccaaacc ccgatcccag agccgcagga acaaccactg
     2401 attccatcgg tagtagtcgg gatgatgcga agccagctcc cggtcccagt cgtacatcgc
     2461 gccgagttgc ttgatctgcc gggtcatcgt ggtgatttgc gcctcggtgt tctccctggg
     2521 gtggacgccg gtctcgatcg cagcattctc cgctggcagg ccgaagctgt cccatcccat
     2581 gggagatagc acgttgtatc cgcgcattgt cttgtacctg gtgagcaggt cgccgaacgt
     2641 gtaattgcga acgtggccct ggtggagggc ccccgatgga taggggtaca tgcagaggtt
     2701 gtagaacttg ggccgcgagg gatcatccgc cgcccgaaag accttttcgg actcccaacg
     2761 ctcctgccac ttggcctcga tttgcttgtg gtcgtatgac atcgaagccc tgagagtagg
     2821 cgacccaacc gctctcctac catccactcg atgagtccag aacgtgttgg cgtcgtcggt
     2881 ggaggtttga tgggctcggg gatcgccgag gtcttcgcca ggtctggcgc ccaggtggtg
     2941 attcacgaaa tcgatgccgg cgccgctgaa gctgcccaag cccgggtcga gtcctctctg
     3001 gccaaagcgg tggaacgggg caagctcgag gtctccctac gcgaccaggc cctggccaac
     3061 atcagggtgt cgacggacct gacagaccag gccgaccgtc aactggtcat cgaggcggcg
     3121 accgaggacg aggcactgaa gaagca
//
LOCUS       DBNHXU010000178         2511 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3827045, whole genome shotgun sequence.
ACCESSION   DBNHXU010000178 DBNHXU010000000
VERSION     DBNHXU010000178.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2511)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2511
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3827045"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..771)
                     /locus_tag="AC161X_04505"
     CDS             complement(<1..771)
                     /locus_tag="AC161X_04505"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012642487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HIR4988842.1"
                     /translation="MSGRLQGKVALITGAGSGIGRETSLLFGSEGASVLAVDLNEEAV
                     AETVSLVQAAGGEISAQKADVSSAEDSEAMVAAAVDRYGHLDVMFNNAGIMHSADDDA
                     VSTEEKIWDLTMDINAKGVFLGCKYGIPALRRAGGGSIINTASFVALLGAATPQVAYT
                     ASKGAVLAMTRELAVVHARENIRVNALCPGPLRTELLMKFLDTDEKKQRRLVHIPMGR
                     FGEAAEMAKAALWLASDDSSYVTGTEFVVDGGITAAYVT"
     gene            complement(768..2147)
                     /locus_tag="AC161X_04510"
     CDS             complement(768..2147)
                     /locus_tag="AC161X_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013927660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR4988843.1"
                     /translation="MPRPPGMISIEELEASVGSGDIDTVVVAFTDHYGRVHGKRFDAN
                     HFLEEISDHGTHGCDYLLTVDMEMEPIPGYTYANWELGYGDFHMVPDLATLRTASWLD
                     RTAFVLCDLEDPRTDDLVTVAPRTILRRQLEKAAESGFTAKGASELEYFLYEDSYREA
                     AEKKYLNMRPAGWYIEDYHLLQGTREEFYNGQVRRHLAASGIPVENSKGEWGRGQHEM
                     NIRYADLLTMADRHTVMKQAMKEIADGTGVSITFMAKPDMTEAGSSCHLHVSLWNDDQ
                     SAFPGDQVFGPVHGSDTFKYFLGGWMSHVPELMVFFAPTVNSYKRYQDLSWAPTRLAW
                     SYDNRTAGFRVVGHDQSLRIECRIPGADANPYLAYSAVLAAGLDGIANKIEPPSIFEG
                     DVYQAQQLPRVPYTLRDATDLFESSDFVKEVFGADVQAHYSHFFRTEQKAFDNSVTDW
                     ERWRYFERI"
     gene            2303..>2511
                     /locus_tag="AC161X_04515"
     CDS             2303..>2511
                     /locus_tag="AC161X_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007670141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HIR4988844.1"
                     /translation="MSTEKKVGSVTYRDVDKGYFEQRGLKRHAGVWMLWALGVAAVIS
                     GDFSGWNFGLDVGGFGGLLIATLVIT"
BASE COUNT          470 a          778 c          761 g          502 t
ORIGIN      
        1 cgtgacgtag gcagccgtga tcccaccatc gaccacgaat tcggtgcctg tcacatagga
       61 ggaatcgtcg ctggccagcc aaagcgcggc tttcgccatc tccgctgcct cgccgaaccg
      121 acccatcggg atgtggacca gccggcgctg cttcttctcg tcggtgtcca ggaatttcat
      181 gagcaactcg gtccgcagcg ggccgggaca tagggcgttc acccggatgt tctcccttgc
      241 gtgcaccaca gctagctctc tggtcattgc aagaaccgca cccttagaag cggtgtaggc
      301 cacctgaggc gttgcggctc ccagtagcgc aacaaaggag gccgtgttga tgatcgaccc
      361 acctcctgcc ctgcgcaggg cgggaatccc gtacttgcag ccgaggaaga cccctttggc
      421 gttgatatcc atcgtgaggt cccagatctt ctcctcggtg gacaccgcat cgtcatcggc
      481 ggagtgcatg atcccggcgt tgttgaacat gacgtcgaga tgcccgtagc gatccacggc
      541 ggccgcaacc atggcctcgc tgtcctcggc cgacgacacg tctgcctttt gcgccgatat
      601 ctctcccccg gcggcctgaa ccagactcac cgtctcggcc acggcttcct cgttgaggtc
      661 gaccgcgaga accgaggcac cttcggagcc gaataggagc gatgtctcgc ggccgatgcc
      721 tgagcccgca ccggtgatca gggcaacctt gccttgcaga cgaccgctca tatccgctcg
      781 aagtatcgcc aacgctccca atcggtgacc gagttgtcga acgccttctg ctcggtgcgg
      841 aagaagtggc tgtaatgagc ctggacgtct gcgccgaaca cttccttgac gaagtcagac
      901 gactcgaaca aatcggtggc atcacgcaat gtgtagggca cccggggcag ctgctgagcc
      961 tggtagacgt cgccttcgaa aatgctcggc ggctcgatct tgttggcaat cccatcgagc
     1021 cccgcagcca gaacggccga ataggcgagg tatgggttgg cgtcggcgcc agggatacga
     1081 cactcgatac gaaggctttg gtcgtgaccg acaacccgga agcccgcagt ccggttgtcg
     1141 tagctccagg caagtcgggt cggcgcccac gataggtctt gatatcgctt gtaggagttg
     1201 acggtcgggg caaagaacac catcaactcg gggacatgtg acatccagcc gccgaggaag
     1261 tatttgaagg tgtcggagcc atgtaccggc ccaaatacct gatcaccggg aaatgcgctc
     1321 tgatcgtcgt tccacaggct gacgtggagg tgacacgacg agccggcctc ggtcatgtcg
     1381 ggcttggcca tgaaggtgat cgaaaccccg gtgccgtcgg cgatctcttt catcgcctgc
     1441 ttcatcactg tgtgccggtc ggccatcgtc agcaggtcgg cgtaccggat gttcatctcg
     1501 tgttggccgc ggccccactc acccttcgag ttctcgaccg ggatccccga agccgccagg
     1561 tggcgacgga cctggccgtt gtagaactcc tcacgggtgc cttggagcag gtggtagtcc
     1621 tcgatgtacc agccggcggg gcgcatgttg aggtacttct tctccgcagc ctctcgatac
     1681 gagtcctcgt agaggaagta ttcgagctcc gacgccccct tggcggtgaa acccgactcc
     1741 gcagcctttt ccaactgccg tctcaagatg gtccgtggcg ccactgtgac cagatcgtcg
     1801 gtgcgcgggt cctcgagatc acagaggacg aatgcagtgc gatccagcca actcgccgtc
     1861 cgcagcgtcg ccaggtcagg gaccatgtgg aagtccccgt accccaattc ccagttggcg
     1921 taggtgtagc cggggatggg ctccatctcc atgtcaacgg tcaggagata gtcacaaccg
     1981 tgggtcccgt gatctgagat ctcctccagg aagtggttgg cgtcgaagcg cttcccgtga
     2041 acccgtccgt aatggtcggt aaaagcaacg acgaccgtgt cgatgtcgcc tgacccgacg
     2101 cttgcctcca actcttcgat actgatcatg cctggcgggc gtggcatccg tctctgctcc
     2161 tcggatctcg gttgatggca gagcgtaggc cggcgcctcc aacgtcgccc ctgagtcagg
     2221 gttgaaaaat ctggtaacca gccgtcaccg gcacaggtcc gattcctagt ctcacccggt
     2281 atccgaaagg gaggtacgtc aaatgtcgac cgaaaagaag gtcggctcgg tcacctacag
     2341 ggacgtcgat aagggctact tcgaacagag gggcctgaaa cggcacgccg gagtttggat
     2401 gctctgggcg ctcggtgtgg ccgcagtcat ctccggcgac ttttccggct ggaacttcgg
     2461 cctggacgtc ggcgggtttg gcgggcttct cattgccacg ttggtgatca c
//
LOCUS       DBNHXU010000179         1815 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5216406, whole genome shotgun sequence.
ACCESSION   DBNHXU010000179 DBNHXU010000000
VERSION     DBNHXU010000179.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1815)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1815
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5216406"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..756
                     /locus_tag="AC161X_04520"
     CDS             <1..756
                     /locus_tag="AC161X_04520"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4988845.1"
                     /translation="WRDGVQALWLIFKFRFIDTKSSEDAGHTTLESLSAAPAVAKWTI
                     EQFSPYLGHRVFEAGSGMGNLTAQLLDRESLIVADIDERHLRSIEKRFGHLENIKAVQ
                     GDLQDPSFYEGLPGDFDSVLCVNVLEHLDDPDVAVSEFYKATRSGGKVLILVPAHEWL
                     FSEADTALGHRTRYTEARLRELLEGVGYVVEDLYQFNRLGVLGWYTNKVFGRTDIAPW
                     QAKLFAFLLPLARLIDGLTFLPGLSLIAIGKRP"
     gene            753..>1815
                     /locus_tag="AC161X_04525"
     CDS             753..>1815
                     /locus_tag="AC161X_04525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988846.1"
                     /translation="MTEPAGSAEAVGVADPKKSRARTAVVVGLVGIALASLIALRVWN
                     AFDQDLTIFVAFGVDSTEINQYAEAKLGREVVKRDTQGHDGKFFFVQANDPLVLSPEE
                     NIAVVDRPLYRSQRMLYPILAGGAGLFSPDVIVWALLIVNLIAIGAGTWAVAQLAMEM
                     GGSPWWGLAFVLNIGFISEMNIDGAGVVAAAAAFGAVAMLLKERLAWAIVLLTIAVLS
                     REAMLIAAAGSAFWLWRRGEKRSAVLVAGIPLAAVALWAVYLRLRIDFDTGLSEVQEI
                     GLPFAGFIQAFKIWVDDPLNLMAGLAIMLLLFMFTRRALMSRHLVGLAFLGFVPLAIL
                     FTRQVWQNYFDITRAVAPVL"
BASE COUNT          294 a          552 c          591 g          378 t
ORIGIN      
        1 tggcgggacg gcgtccaggc cctgtggctg atcttcaaat tccggttcat cgacaccaag
       61 tcatccgaag acgcggggca caccactttg gaaagcctct cggctgcccc ggcggtggcc
      121 aagtggacga tcgagcagtt cagcccgtat ctggggcaca gggtgttcga ggccggctcg
      181 ggcatgggaa atctgactgc gcagctgctc gaccgcgaga gcctcatcgt ggcggacatc
      241 gacgagcgcc atctgcgatc aatcgaaaag cgattcgggc acctggagaa catcaaagcc
      301 gtccaagggg acctccagga tccgagcttc tatgagggcc tgcccgggga tttcgacagt
      361 gtcctctgtg tcaacgtgct cgagcatctc gacgatcctg atgtcgcagt ttccgagttc
      421 tacaaggcga ccaggtccgg cgggaaggtg ttgattctcg ttccggctca cgagtggctg
      481 ttctcggaag cggacaccgc tcttggccat cggacgagat acaccgaggc tcggctgcgt
      541 gagcttctcg aaggcgtggg gtatgtggtc gaggacctgt atcagttcaa ccgcctcggc
      601 gtgctcggct ggtacaccaa caaggtgttc gggcggaccg acatcgcccc ttggcaggcg
      661 aagctgttcg cctttctgct tcccctggct cggctgatcg acggtctgac gttcctcccc
      721 ggtctgagcc tgatcgcaat cggcaagagg ccgtgaccga gcccgccgga tcggcagaag
      781 cggtcggagt ggcggatcca aagaagtcga gggctcgaac cgcagtcgtg gtcggcctcg
      841 tcgggatcgc gctggcgtcg ttgatcgcct tgcgtgtttg gaacgccttc gaccaggacc
      901 tcacgatctt cgtcgccttt ggggtcgact ccaccgagat caaccagtac gccgaggcca
      961 aactgggacg ggaggttgtc aagcgggaca cccagggcca cgacggcaag ttcttcttcg
     1021 tccaggccaa cgatccgttg gtcctctcgc ccgaggagaa catcgctgtg gtcgatcggc
     1081 cgctctatcg aagccaacgg atgctgtatc cgatcttggc cgggggagcg gggctgttct
     1141 cgccggatgt gatcgtctgg gcgctgctca tcgtcaatct gatcgcgatc ggagccggca
     1201 cctgggcggt cgcccaactc gcgatggaga tgggaggctc gccctggtgg ggccttgcct
     1261 tcgtcctcaa catcgggttc atcagcgaga tgaacatcga cggtgccggt gtggtcgcgg
     1321 cagcagccgc gttcggagct gtggcgatgc tcctcaagga gcgcttggcg tgggcgattg
     1381 tcctcctcac catcgccgtg ctctcccggg aggcgatgct gatcgccgct gcgggctcgg
     1441 ccttctggct gtggcgcaga ggggagaagc gaagcgcggt cctggtggct gggattccgt
     1501 tggcggcagt ggccctttgg gcggtctacc tccggttgcg gatcgacttc gacaccgggc
     1561 tgtcagaggt ccaggagatc ggcctcccgt tcgccggctt cattcaggct ttcaagatct
     1621 gggtggatga cccgctcaat ctcatggccg gccttgccat catgctcctg ctcttcatgt
     1681 tcaccagaag ggctctgatg tcacgtcacc tcgtggggtt ggcctttctc ggcttcgttc
     1741 ctctggccat cctcttcacc cgacaggtgt ggcagaacta cttcgacatc acccgggcgg
     1801 tcgccccggt gctca
//
LOCUS       DBNHXU010000180         1764 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1643633, whole genome shotgun sequence.
ACCESSION   DBNHXU010000180 DBNHXU010000000
VERSION     DBNHXU010000180.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1764)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1764
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1643633"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..365)
                     /locus_tag="AC161X_04530"
     CDS             complement(<1..365)
                     /locus_tag="AC161X_04530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020421712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HIR4988847.1"
                     /translation="MAEFATLHDAIADLLPDGSTVAMEGFTHLIPFAAGHEVIRQEKR
                     DLTLVRMTPDLVYDQLIGMGCAGKLIFSWGGNPGVGSLHRFRDAVENGYPGPLALEEH
                     SHAAMAARYTAGASGLPFAV"
     gene            complement(419..1225)
                     /locus_tag="AC161X_04535"
     CDS             complement(419..1225)
                     /locus_tag="AC161X_04535"
                     /EC_number="1.13.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF012976.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="intradiol ring-cleavage dioxygenase"
                     /protein_id="HIR4988848.1"
                     /translation="MDHDHIEEPSNEELRLLSRRSAIKLLGGAGLAMIAACATGSVVT
                     TTAAAAGSSSTTGATGTTIGGATATTFGGAGSCVLIPEETAGPYPLDLSGDEAFFRTD
                     ITEGHAGVPLALTLALVNASADCAPISGARVDVWHCDADGSYSGYSQQGTDTTGETFC
                     RGIQMSDNSGQVIFQTIYPGWYPGRITHIHFEVFLDNGLVATSQIAFPPEVTDAVYAV
                     DPYAAKGPNTSVPTFADDMVFSDGTQYQMATVAGDPTAGYAATLLVGVAA"
     gene            1293..1733
                     /locus_tag="AC161X_04540"
     CDS             1293..1733
                     /locus_tag="AC161X_04540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4988849.1"
                     /translation="MPSKTFQQEAIANAPAAEVWAALNQPVAWESVPGVDRIIDSVID
                     AEGQLQGFSFESTAAGKRYQGRATPAGRETEKVIAWNIETSEVRGRIIVALSPDEGTT
                     QVAVTLRMESVGVLSSMFFPVIVAAIDDGFPQTVRDFAESLSRI"
BASE COUNT          345 a          536 c          590 g          293 t
ORIGIN      
        1 accgcaaacg ggagaccgga cgcacccgct gtgtagcgag ccgccatggc ggcatgactg
       61 tgctcttcga gggcgagcgg ccccggataa ccgttctcaa cggcgtcccg gaaacgatgc
      121 aaagagccga cgccgggatt cccaccccaa gagaagatca gctttccggc acagcccatc
      181 ccgatcagtt ggtcgtatac caaatcgggt gtcatccgga ccagggtcaa gtctcgcttc
      241 tcctggcgga tgacctcgtg ccctgccgca aacggaatga gatgggtgaa gccttccatg
      301 gcgacggtgg aaccgtcggg gagcagatcg gctatcgcat catgaagggt tgcgaactcg
      361 gccactcaag cgcccgccgt tgagacctgt cccatgcgct gatcgtaccg ggaggtctct
      421 acgcggcgac cccaaccaag agggtggcgg catatccggc ggtcggatcg ccggcaacgg
      481 tggccatttg atactgggtt ccatcgctga acaccatgtc gtcggcaaag gtgggaaccg
      541 acgtgttggg gcccttggcc gcatagggat cgaccgcata caccgcgtcg gtgacctcgg
      601 gagggaaggc aatctgcgac gtcgcgacca gcccattgtc gaggaagacc tcgaaatgga
      661 tgtgggtgat gcgcccggga taccagccgg gatagatggt ctggaagatg acctggccgc
      721 tgttgtccga catctgaatg ccacggcaaa aggtctcccc ggtggtgtcg gtgccctgct
      781 gggagtagcc ggaataggag ccatcggcgt cgcaatgcca cacatcgaca cgggcaccgc
      841 tgataggcgc gcaatcggcc gaggcattga ccagcgccag cgtgagcgcc aggggcaccc
      901 cggcgtgacc ctcggtgatg tcggtgcgga agaaagcctc atcaccggag agatcgagtg
      961 ggtagggacc ggcagtctcc tcgggaatga ggacacaact gccggcgccg ccaaatgtcg
     1021 tcgcggtcgc tccgccgatt gtggtcccgg tggcgccggt ggtgctggag gagcccgcag
     1081 ccgcggcagt ggtggtgacg acagaacccg tcgcacaggc agcgatcatg gcgagaccgg
     1141 cgccaccgag caatttgatg gccgaacgcc ggctgagcag ccgaagctcc tcgttgctcg
     1201 gttcctcgat gtgatcgtga tccatgtcac ccacagttca ccatgaatcc tggacaagcg
     1261 atctgcccgc ctcttccgta atgtccccca ccgtgccgag caagacgttc cagcaggagg
     1321 ccattgccaa tgcgcctgcc gccgaggtgt gggcagcact caaccagccg gtggcctggg
     1381 agtcggtgcc tggagtggac cggatcatcg actcggtgat cgacgcggag ggacagctcc
     1441 agggcttttc cttcgagagc accgcagccg gcaagaggta ccagggccgc gccacccccg
     1501 ccggccgaga gacggaaaag gtgatcgcct ggaacatcga gacctcggaa gtccgaggac
     1561 ggatcatcgt cgccctgagc ccggatgaag ggacgactca ggtcgcggtg accctccgca
     1621 tggagagtgt gggggtgctg tcctcgatgt tcttccccgt gatcgtggcg gccatcgacg
     1681 acggattccc ccagacggtg cgggattttg cggagagtct gagtcggatc taggtccgga
     1741 tcaggaagcc tcttcgacgg cgag
//
LOCUS       DBNHXU010000181         6981 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1843134, whole genome shotgun sequence.
ACCESSION   DBNHXU010000181 DBNHXU010000000
VERSION     DBNHXU010000181.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6981)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6981)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6981
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1843134"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..608)
                     /locus_tag="AC161X_04545"
     CDS             complement(<1..608)
                     /locus_tag="AC161X_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4988850.1"
                     /translation="MLRPTALVLLLLALIVGPAVSAYAELPPGGTFTDDDGSIFEGAI
                     EAIAAEGITEGCNPPENTLYCPDDLVTRGEMAVFLVRAFGYTESGWGNFFTDDDGQFF
                     ETSADRLKMAAVTLGCNPPANDRYCGERHVSRGEMAAFLVRALGLVQSSGPNLFIDDD
                     GSTFELAINRLATAGITKGCNPPVNDRFCPNDFVTRGQMAAFL"
     gene            complement(628..1302)
                     /locus_tag="AC161X_04550"
     CDS             complement(628..1302)
                     /locus_tag="AC161X_04550"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4988851.1"
                     /translation="MRKPICVLVLVLLALAVSPVGAAYAELPPGGTFTDDDGSIFEGA
                     IEAIAAEGITEGCNPPANTSFCPDDFVTRGEMAVFLVRAMGYTDNGGGDLFIDDDGLF
                     YEDSADKLIVAGVTNGCTSFPPNNRFCGERYVTREVMAAFLVRAFGYTDDGGGNLFID
                     YYGTFFGYAIDRLFVAGITQGCNPPVNDRYCPLDPVTRGEMAAFLTRALHLSPVYPPA
                     SGSVAP"
     gene            complement(1341..1417)
                     /locus_tag="AC161X_04555"
     tRNA            complement(1341..1417)
                     /locus_tag="AC161X_04555"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1381..1383),aa:Val,seq:tac)
     gene            1481..2422
                     /locus_tag="AC161X_04560"
     CDS             1481..2422
                     /locus_tag="AC161X_04560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4988852.1"
                     /translation="MDSSDAPRFFGVLVTYRRPDQLATTLARIQAQTRQLDRLIVVDN
                     GSDRVTASAVETQTAEYIDAGDNLGPAGGYALGMNRLLEQARDGDWIFLFDDDDPPFF
                     DDAIENAARFAQTTAETNPEVGAIGISGGRFDLNAGKVIRVGDSEIHDVVSVDHITGG
                     GLPAYLVGAVREVGVPLPQLFFGFEELEYGLRMTKAGHLLFADGEQWARRKAIKRDAG
                     LLPPEQVSETRSTSTNLRVSEPSWRRYYSLRNLIFILRLNGASGTALRVAVTRGILKP
                     LANLFTSPSLAWANLKLNWKAIGDGWRGRMGRTVIPG"
     gene            complement(2419..3195)
                     /locus_tag="AC161X_04565"
     CDS             complement(2419..3195)
                     /locus_tag="AC161X_04565"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR4988853.1"
                     /translation="MPDRIRNGIVKIERSAKKLPTPLRQIGLGMVGFFTRMLGSITSV
                     ETRQPVVALTLDDGPDPEVTPKVLDLLNERGIKATFFILSERAEAHPELARRLVAEGH
                     EAALHGANHDNLTNLGIGQAVERVRGGRRRLNQVVGSPVRLFRPPYGAQNLRTFALSR
                     LAGMKVVMWSADPNDWDEIGLEEIVARTIHQVVPGGILLLHDGWTPDPNGDGSRPTGD
                     KIEVIKAILDALDGAGLTLTTVSDLLKSSPARKRVWFESG"
     gene            3327..4253
                     /locus_tag="AC161X_04570"
     CDS             3327..4253
                     /locus_tag="AC161X_04570"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR4988854.1"
                     /translation="MPNPRVTIVIPTRNRQQLADRALGSALAQTISDLEVIIVDDGSS
                     PPFAQSIPDPRVRLVRNETSAGPSAARNTALGMANGEWITFLDDDDLIIPEMLEASLN
                     AAATSSLPTPISVLSGASIVEPDGTVLKTRMPPTLIRGQHFFLEDLPDGGSFQTHATL
                     VAPTEVVRAIGGFDESLRASEHDDFFLRLNAASSIQGAPIVSYQITAHSGPRLSKAIL
                     ERAHGMEQTVEKHEATFRQHPRRFARYLTTMGVTYLRAGEWGPAVRATTRSITVDPTR
                     IDSYRWWLASLAGPWTLSLARRLRRGPGEPEV"
     gene            4256..4990
                     /locus_tag="AC161X_04575"
     CDS             4256..4990
                     /locus_tag="AC161X_04575"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013672.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HIR4988855.1"
                     /translation="MARYRKTARFVRSLGLERVAHAFAGSLCEVKTDRPVFHITFDDG
                     PNPEVTPRILEVLEEFDAPATFFLLAENAERHPELVHKIMDRGHELGHHSRTHLRLST
                     APWGRLPDEIWKARRDLERVAGRTIRLFRPPYGAHGLRSLYMVKGAGMRTVLWSVDSE
                     DWKGLSPDDPLQHTKAKIGNGGILLLHDRPATDTVEGDRAAGHLPKEDLTRAYLVELA
                     QRGLKTVSLAELLDGGQEVRRAKVAK"
     gene            4990..5745
                     /locus_tag="AC161X_04580"
     CDS             4990..5745
                     /locus_tag="AC161X_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4988856.1"
                     /translation="MTFAERVDPRTGLRERFEYFGSPPDLKLGQLLSPASPSRGVLIA
                     GPIDADRALRPQIEEISASLGGSGHVVARFDYRGQGQSFGETGDLTLELMAEDVSEAI
                     GWIRESAAVEHLAMLGVGLGATAAAMAEGASQLPLVIWDPVTDIDLFLRGLARSKLSI
                     EMGFVGDGASYDEGSLADVVADDLLNEELDRAGFFDAGGFPVASAIVDSARRHSLSDV
                     MVRSAETMIVPFGPGRAMIPDTVRLIQESFHKQ"
     gene            5742..6473
                     /locus_tag="AC161X_04585"
     CDS             5742..6473
                     /locus_tag="AC161X_04585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4988857.1"
                     /translation="MTVSVTETPVFFGQADEFFGISSSGPEAGSLGAVICLTPSMADN
                     PVGSRLARKLATAGVPALRFDLAGHGHSHESRHGDAWDGTSFQAAAEWMREMAIESFV
                     LIGGTTGANACLTASSAMTDLRGLVFNRLSTKRPVGRVSRIVRPPRGRPATPTVISQL
                     QDVIERGVPTLVVSGAADTDQARIRRLFGDREPPLDRTSLQMATVPSRTLKGFRSVPA
                     QEEFVDVVVRWISDLAGTTPSDLAL"
     gene            complement(6456..>6981)
                     /locus_tag="AC161X_04590"
     CDS             complement(6456..>6981)
                     /locus_tag="AC161X_04590"
                     /inference="COORDINATES: protein motif:HMM:NF024819.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminoglycoside adenylyltransferase
                     domain-containing protein"
                     /protein_id="HIR4988858.1"
                     /translation="APRVPARSLECWVITTETAGAGGSMRDVEVMVNTHPSEPMTVDG
                     ATGAVGVPVVELDLVNETGRSFYGPSPSDLVAAIPRSAVLASMTENLRVETADSSEHY
                     SVLNASRSLAYMFTGKHISKLEGGRWVLARGSAPKVVEDAVQTQMGLAPERALTRAGG
                     RYVSEIMRQLESEI"
BASE COUNT         1339 a         2289 c         2078 g         1275 t
ORIGIN      
        1 aggaacgcag ccatctgccc tcgggtgacg aagtcattcg ggcagaaccg gtcgttgacc
       61 ggcggattgc aaccctttgt gattccggcc gtcgccagtc ggttaatggc gagctcaaaa
      121 gtcgagccgt catcatcgat gaagagattc ggaccgctcg attggaccaa tcccagggca
      181 cggaccaaga aggctgccat ctcgcccctc gagacgtgtc gctcaccgca atacctgtcg
      241 ttggcgggtg gattgcagcc caaggtcact gctgccatct ttagtcggtc ggcagaagtc
      301 tcaaagaact gtccgtcatc gtccgtgaag aaattgcccc agccactctc ggtgtaaccg
      361 aaggcgcgaa ccaagaagac ggccatttca cccctggtca ccaaatcatc tgggcagtag
      421 agggtgttct ccggcgggtt acatccttcc gtgatgccct cggcggcgat ggcctcgatc
      481 gctccttcga agatgctccc gtcgtcatcg gtgaacgttc caccaggcgg caactcggca
      541 tatgccgaca ccgcagggcc gacaatcagt gccagtaata ggaggacgag cgcggttggt
      601 cgaagcatgt caagctcaga gggaccgtta gggggcgacc gaaccagacg ccggcgggta
      661 gaccggggac aagtgaaggg ctcgggtcag gaacgccgcc atttcgcccc tggtcacggg
      721 atcgagcggg cagtatctgt cgttcactgg agggttacag ccctgggtga tcccagcgac
      781 aaagagccga tcgatcgcgt agccgaaaaa ggtgccgtag tagtcgatga agaggttccc
      841 acctccgtcg tcggtatagc cgaatgctcg gaccaagaac gcagccatga cttcacgggt
      901 gacatatcgt tcaccacaga agcggttatt tggtgggaaa gaagtgcacc cgttggtcac
      961 accggcgacg atcaacttgt ctgcagagtc ctcgtagaac agcccgtcgt catcaatgaa
     1021 caggtccccg ccaccgttgt cggtataacc catggcccgc accaagaaga ccgccatctc
     1081 accgcgggtc acgaagtcgt ctggacaaaa actggtattg gctggtgggt tacacccttc
     1141 cgtgatgcct tcggcggcga tggcctcgat cgctccttcg aagatgctcc cgtcgtcatc
     1201 ggtgaacgtt ccaccaggcg gcagctcagc atatgccgcg cccacagggg agacagccag
     1261 cgccagcaat acgaggacga gcacgcatat tggcttccgc atcaacactc caatcgagtt
     1321 cgtggctcct aggcttcgga tggtgggcgt tgagggactt gaacccccga cccctgcctt
     1381 gtaagggcag tgctctagcc aactgagcta aacgcccggg acggcggcga ttctacggga
     1441 accagggcat tctcccatcc gtctctaatc tcgcctctgg atggacagca gcgacgctcc
     1501 ccgtttcttc ggcgtgctgg tcacctatcg acgacccgat cagcttgcca ccaccctcgc
     1561 ccgtattcag gcccagaccc gccaactcga ccgtctgatc gtggtcgaca acggcagcga
     1621 ccgggttacg gcgtcggccg tcgagaccca aaccgctgaa tacatcgacg ccggagacaa
     1681 cctcggaccg gctggtgggt acgccctcgg catgaaccgt ctgctcgagc aagcacgtga
     1741 cggggactgg atcttcctgt tcgatgacga cgacccgcca tttttcgacg acgccatcga
     1801 gaacgcggct cgctttgccc aaacaaccgc agagaccaac cctgaggtcg gcgcaatcgg
     1861 catcagcggg ggccggttcg acctgaatgc cggaaaggtg atcagggtcg gcgactccga
     1921 gattcatgat gtcgtctcgg tcgatcacat caccggtgga gggctccccg cctatctagt
     1981 cggagccgtg cgcgaagtcg gagtccccct cccgcaactc ttcttcggat tcgaggaact
     2041 cgagtatgga ttgcggatga ccaaagccgg ccatttgctg tttgcagacg gtgagcaatg
     2101 ggcgagacgc aaggccatca agcgggacgc cggcctgctt ccacccgagc aggtctcaga
     2161 gacgaggtcg acaagcacca acctcagagt ctccgagccg tcctggcgcc gctactacag
     2221 cctccgcaac ctgatcttca tcctccgcct gaacggcgcg tccggcaccg cacttcgggt
     2281 ggcggtcacc agggggatcc tcaaaccttt ggcgaatctc ttcaccagtc cgtcacttgc
     2341 ctgggccaat ctcaagttga actggaaggc gatcggcgac ggatggcgtg gtcggatggg
     2401 gcgaacggtg attcccggct agccgctttc gaaccacacc cgcttgcgcg ccgggctcga
     2461 tttgagcaga tccgatacgg tcgtcagcgt cagcccggcg ccgtcaagag cgtcgagaat
     2521 cgccttgatc acttcgatct tgtcccccgt agggcggctg ccatccccgt tcgggtccgg
     2581 ggtccagccg tcatggagga ggaggattcc ccccggcacc acctggtgaa tggtgcgggc
     2641 gacaatctcc tcgagaccga tctcgtccca gtcgttggga tcagccgacc acatcacaac
     2701 cttcatcccc gcgagccggg acaaggcaaa ggtgcgcaaa ttctgcgcgc cgtagggagg
     2761 acggaataga cgcaccggtg atcccaccac ctggttgagg cgccgcctgc cgccccgcac
     2821 ccgctccacg gcctggccga tgcccaggtt ggtcaggttg tcatggttcg ccccgtgaag
     2881 agcggcctcg tgaccctcgg ccaccaagcg tctcgccagt tcagggtgag cctcggcacg
     2941 ctcggagagg atgaagaagg tcgccttgat gccccgctcg ttgagcagat caagaacctt
     3001 cggtgtgacc tcgggatcag gcccatcgtc gagtgtcagc gctacaaccg gctggcgggt
     3061 ctcgacgctc gtgatcgatc ccagcatcct cgtgaagaat ccgaccatgc cgaggccgat
     3121 ctgacgcagc ggtgttggaa gcttcttcgc cgatcgctcg atcttgacaa ttccgttgcg
     3181 aatccggtcg ggcaacgaat acctctttcc taagccctgc tcatagtctc gccaggtttg
     3241 ggccactggc aacaggggcc tgaaggggcc ttttcccttg acccaccccc cgacccgatc
     3301 aaactcaaca ccggtcagtg tcaaaaatgc ccaatcccag agtcaccatc gtcatcccca
     3361 cccggaaccg ccagcaactg gccgaccggg ctttgggcag tgccctggct caaaccatct
     3421 ctgacctcga agtgatcatt gtggacgatg ggtcgtcgcc tccctttgcc cagtcgatcc
     3481 ccgacccacg agtcagactc gttcgcaacg agacctcggc cggtccttcg gcggcccgca
     3541 acaccgcgct tggcatggca aacggcgagt ggatcacctt tctcgacgac gacgacttaa
     3601 tcattcccga gatgctcgag gcctcgttga acgcggccgc cacctcttcc ctccccactc
     3661 cgatctcagt cctctccggc gcatcgatcg tggagcctga tgggacggtt ctcaagacac
     3721 gaatgccacc caccttgatc cgcggccagc atttctttct cgaagatctg ccggacggag
     3781 gcagtttcca gacccacgcc acattggtgg caccgaccga ggtggtgcgg gccatcggcg
     3841 ggttcgacga gagcctgcgt gccagcgagc acgacgactt cttccttcgt ttgaacgccg
     3901 ctagctccat ccagggagcg ccaatagtga gctatcagat caccgctcac tccggccccc
     3961 gtctctccaa ggcgatcctg gagagagcac atggcatgga gcaaaccgtc gagaagcacg
     4021 aagccacctt ccgccagcat ccccggaggt ttgccaggta cctgaccacc atgggcgtca
     4081 cctacctccg agccggcgag tgggggccgg cagtcagggc cacaacccga tcgatcaccg
     4141 tcgaccccac ccgcatcgac tcctatcgtt ggtggctggc atcgctggca ggtccatgga
     4201 ctctgagtct cgcccgacgg ttgcggcgtg gccccggcga accggaggtc tgaacatggc
     4261 tcgatatcgc aagactgccc gtttcgttcg ctcgctcgga ctggaacggg tggcacacgc
     4321 cttcgcaggc tcgctctgcg aggtgaaaac cgaccgaccg gtgttccaca tcaccttcga
     4381 tgacgggccg aaccccgagg tgaccccgcg gatactcgag gtcctcgaag agttcgacgc
     4441 tccggcgacg ttcttcctcc tggccgagaa tgccgaaagg cacccggagt tggtgcataa
     4501 gatcatggat cgcggccatg aactgggtca tcacagccgg acccatctcc gcctgtcgac
     4561 ggcaccgtgg ggccgactgc ccgacgagat ttggaaggcg cgtcgcgacc tggagcgggt
     4621 cgccggccgg accatcaggc tgtttcgccc gccgtacgga gctcacggtc tccgctctct
     4681 ctacatggtg aaaggcgccg gcatgcgaac ggttctctgg tcggtcgact ccgaggactg
     4741 gaagggcctg agcccggacg accctctcca gcacaccaag gccaagatcg gaaatggcgg
     4801 aatcctcttg ctccacgacc gcccggccac tgacacagtc gaaggcgacc gggcggccgg
     4861 ccacctaccc aaagaagacc tgacccgcgc ctatctggtc gaactggcgc aacgcggact
     4921 aaagaccgtg tccctcgccg aattgctcga cggtggccag gaggttcggc gggccaaggt
     4981 cgccaagtga tgaccttcgc ggagcgcgtc gatcccagaa ccggcctccg cgagcgcttc
     5041 gaatacttcg gctctcctcc ggacctcaaa ctgggtcagc ttctgtcacc ggcctccccc
     5101 tctcgcggag tcctcattgc cggccccatc gatgccgatc gggccctgag gccccagatc
     5161 gaggagatct cggcctcgct cgggggatcg ggacatgtgg tggctcgttt cgactaccgg
     5221 ggccagggac agagtttcgg cgagacgggc gatctcacac tcgagctgat ggcggaagat
     5281 gtcagcgagg ccatcggatg gatccgagag tcggctgcgg tcgaacatct cgccatgctc
     5341 ggcgtcgggc tgggcgccac cgccgctgcg atggcggaag gtgcgagtca gctgccactt
     5401 gtcatctggg accccgtcac cgacatcgac cttttccttc gcggtctggc ccgatccaag
     5461 ttgtctatcg aaatgggctt cgttggtgac ggtgcgtcct acgacgaagg aagcctcgcc
     5521 gacgtggtgg ccgatgacct gctcaacgaa gaactcgacc gtgccggctt tttcgatgcc
     5581 ggaggttttc cagtagcgag cgccatcgtc gactcagcac gcaggcactc gctttccgac
     5641 gtgatggtca gatcggcgga gacgatgatc gtcccgttcg gcccgggccg agccatgatc
     5701 ccggacacgg tcaggttgat ccaggagagc tttcacaagc aatgaccgtt tccgtcaccg
     5761 agacgccagt gttcttcggt caggccgacg agttcttcgg catttccagc agcggcccag
     5821 aggcagggtc tcttggtgcc gtgatctgtc tcaccccctc gatggccgac aacccggtcg
     5881 gttcccgact cgcccggaag ctcgccaccg caggggtccc ggctttgagg ttcgacctcg
     5941 ccgggcatgg ccatagccac gaatcgcgcc atggcgacgc ctgggacggc accagtttcc
     6001 aggcggcggc ggagtggatg agagaaatgg cgatcgaatc attcgtgctg atcggcggca
     6061 cgactggggc caatgcctgc ctaaccgcgt ccagtgcgat gactgacctc cgtggactcg
     6121 tcttcaaccg gctgtcgacc aagcggcctg tgggccgggt gagccgaatc gtccgaccgc
     6181 ctcggggccg acccgccact ccgacggtca tctctcagct tcaggatgtg atcgagcgtg
     6241 gggtcccgac actcgtggtc agcggcgcgg cggacaccga ccaggcacgg atacgccgac
     6301 tgttcggcga tagagagccg ccactcgatc ggacttcgtt acagatggca accgtgccca
     6361 gcaggaccct caaagggttc aggtctgtcc ccgcacaaga agagttcgtg gacgtggtcg
     6421 tgcgatggat cagtgacctc gctggaacca ccccgtcaga tctcgctctc tagctgtcgc
     6481 atgatctcgc tcacatagcg accaccggcg cgagtgagag ctcgctccgg cgcaagaccc
     6541 atctgagttt gaaccgcatc ttcgaccact ttgggcgccg agcctcgtgc gaggacccac
     6601 cgcccgccct ccagcttcga aatgtgcttc cccgtgaaca tgtaggccag gctccgggag
     6661 gcattgagca cgctgtagtg ctcggaagag tctgcagtct cgactctgag attttcggtc
     6721 atggatgcga gaaccgcgga ccggggaatg gcagcgacca aatcggatgg cgacggcccg
     6781 tagaagctcc gaccggtttc gttcactaga tccaactcca ccaccggcac tccgaccgcc
     6841 cccgtggcac catcgacagt catcggctcc gacggatgag tgttgaccat cacctcgaca
     6901 tcgcgcatcg agcctccagc gcccgcggtc tcggtggtga tcacccaaca ctccaggctc
     6961 cgggcaggaa ccctgggcgc c
//
LOCUS       DBNHXU010000182         1767 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_57470, whole genome shotgun sequence.
ACCESSION   DBNHXU010000182 DBNHXU010000000
VERSION     DBNHXU010000182.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1767)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1767)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1767
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_57470"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..633
                     /locus_tag="AC161X_04595"
     CDS             <1..633
                     /locus_tag="AC161X_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014680208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citrate/2-methylcitrate synthase"
                     /protein_id="HIR4988859.1"
                     /translation="LNMLLILHADHGQNCSSSAVRLVGSGQANIFSSVSAGIHALSGP
                     LHGGANEAVLAMLEMIVADGGEVSKWVDRAKDKDDPFRLMGFGHRVYKNFDPRARIIK
                     QATDEVLDALGVDDSLLDVALQLEEVALSDDYFISRKLYPNVDFYSGILYRAMGFPID
                     MFTVLFALGRLPGWISQWRELMADSETKIFRPRQVYVGPAPRDFVPLDER"
     gene            complement(635..1126)
                     /locus_tag="AC161X_04600"
     CDS             complement(635..1126)
                     /locus_tag="AC161X_04600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988860.1"
                     /translation="MTEPESPRVDREGIHIERDLADSISIEEELDSNVVGPYRFPSPM
                     RRRIAGWVFVVGAVLTVVLLDDGWIPAIGFVALALWQFASAWPLNIDEHRALSIAGAA
                     VDFPVGHASAAVTFRGFRSVPRWSVVLYSAVEPPDQRALVVVDAVTGELAEEPYVEDV
                     PEV"
     gene            complement(1123..>1767)
                     /locus_tag="AC161X_04605"
     CDS             complement(1123..>1767)
                     /locus_tag="AC161X_04605"
                     /inference="COORDINATES: protein motif:HMM:NF015113.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="His/Gly/Thr/Pro-type tRNA ligase C-terminal
                     domain-containing protein"
                     /protein_id="HIR4988861.1"
                     /translation="AKSVGEQSRWEVSEKALADSLDQKGVRYRIAEGEGAFYGPKIDV
                     HVKDAIGRRWQVSTIQLDFTLPERFGLEYTASGNIQERPYMIHCAKAGSIERFVGVLV
                     EHYAGAFPMWLAPVQVSVIPVADRHHEYANSVAAKLRDPGLRVEVDGSEETVGEKIRR
                     ALSQKHPAVVVVGDQDVDNSTVGLRLYGEERDTRDVPLIEATNRLVDLAKRPVT"
BASE COUNT          317 a          583 c          545 g          322 t
ORIGIN      
        1 ctcaacatgc tgctgatcct ccacgctgac cacggtcaga actgctcatc gtccgcggtg
       61 cgtctggtcg gctctggtca ggccaacatt ttcagttcgg tttcggcagg catccatgcc
      121 ctctccgggc cgctgcacgg gggggccaac gaggccgtgt tggccatgct cgagatgatc
      181 gtggctgacg gcggggaggt ctcgaagtgg gtcgaccggg ccaaggacaa ggacgacccg
      241 ttccgactga tgggcttcgg gcatcgggtc tacaagaact tcgatccccg ggcccggatc
      301 atcaagcagg cgaccgacga ggtcctcgac gccctggggg tggacgattc cctgctcgac
      361 gtcgccctgc aactcgagga agtcgccctc tccgacgact acttcataag ccgcaagctc
      421 tatccgaatg tggacttcta ttcggggatc ctctaccggg cgatgggctt tccgatcgac
      481 atgttcaccg tcctcttcgc cctcgggcgg ctgccggggt ggatatcgca atggcgcgag
      541 ctgatggccg actcggagac caagatcttc aggccgcgcc aggtgtatgt cgggcccgcc
      601 cctcgggact tcgtcccctt ggacgagcgc tgagtcagac ctcgggaacg tcctccacgt
      661 aaggctcttc ggcgagctcg ccggtaaccg catcgaccac cacaagcgcg cgctggtctg
      721 gaggctccac cgctgaatag agaaccacgc tccatctcgg caccgagcga aacccgcgaa
      781 aggtgactgc ggccgaagcg tgacccaccg gaaagtcgac cgcggcgcct gcaatcgaga
      841 gggcgcgatg ctcgtcgata ttgagcggcc aggccgaggc gaattgccaa agtgccaggg
      901 ccacgaaccc gattgctgga atccatccgt cgtcgagaag cacgactgtg agcaccgccc
      961 cgaccacgaa cacccatcct gcgatccggc gacgcatcgg tgacgggaac cgatacgggc
     1021 cgaccacgtt ggagtcgagc tcctcctcga tcgagatgga gtcagccaga tctcgctcga
     1081 tgtggatccc ctcccgatcg acccgtggtg actccggttc ggtcacgtaa caggtctctt
     1141 cgccaggtcg acgagtcgat tggtggcctc gatcagcggg acgtctctgg tgtcccgctc
     1201 ttcgccgtag aggcgcagcc ccacagtcga gttgtcgacg tcctggtcgc ccacgacgac
     1261 gacggccggg tgcttctggc tgagagcgcg acggatcttc tcccccacgg tctcctcgct
     1321 cccgtcaacc tcgacccgca accccgggtc acgcagcttt gcggcgacgg agtttgcgta
     1381 ttcgtggtgg cggtcagcca ccgggatcac cgagacctgc acgggcgcca gccacatcgg
     1441 gaaggcgccg gcatagtgct ccaccaacac cccaacgaag cgctcgatgg agcctgcctt
     1501 ggcacagtgg atcatgtagg ggcgctcctg gatgttgccc gaagcggtgt attcgaggcc
     1561 aaaccgctca ggaagggtga agtcgagttg gatcgtcgag acctgccagc gacgcccgat
     1621 cgcgtccttg acgtggacat cgatctttgg gccgtaaaag gccccttcgc cttcggcaat
     1681 tcggtagcgg acgcctttct ggtcgaggga gtcggcgagc gccttctcac tcacctccca
     1741 acgtgactgc tcaccaaccg atttcgc
//
LOCUS       DBNHXU010000183         1504 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_258103, whole genome shotgun sequence.
ACCESSION   DBNHXU010000183 DBNHXU010000000
VERSION     DBNHXU010000183.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1504)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1504
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_258103"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="AC161X_04610"
     CDS             complement(<1..464)
                     /locus_tag="AC161X_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019946128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HIR4988862.1"
                     /translation="MATPITMSPEGLVVPDDPIVPFIEGDGTGPDIWAASVRVFDAAV
                     GKAYGDSRKVNWTEVLAGEKAHNEVGSWLPDETVEAFETYLVGIKGPLTTPVGGGIRS
                     LNVALRQILDLYACVRPIRWFEGVPSPVKEPGLVDMIIFRENIEDVYAGLEL"
     gene            complement(544..>1504)
                     /locus_tag="AC161X_04615"
     CDS             complement(544..>1504)
                     /locus_tag="AC161X_04615"
                     /inference="COORDINATES: protein motif:HMM:NF012613.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4988863.1"
                     /translation="ILDILPEPNVRNNPWGDDPSSFKTAFRNIATQARAEIGSSRVRV
                     VFSGNRSMSSSRYSQSQWGTGGFGLYWPGASYADAAGVGGYPATGGSNVGYHKSAVDE
                     MANAVGPGIPILISGGAAPNLPNEAAQVQYAQALASLAVSHPQIVGVQWDDLVKGSLD
                     LRVSTTSGLQTGFAAATQGARTGGVDWLFSSSVKTWAAARNAANPFDDSSASIFSDSI
                     NWLSATGITQGCGPRKFCPDDPVTRGQMAAFLVRALKLPAPPSPRVFTDTRGHIFESA
                     ISRLAYAGITLGCNPPTNTRFCPNNNVTRGEMAAFLVRAGLAD"
BASE COUNT          279 a          474 c          481 g          270 t
ORIGIN      
        1 tccaactcga ggccggcata gacgtcctcg atgttctccc tgaagatgat catgtcgacc
       61 aggcccggct ccttgaccgg tgagggcacc ccctcgaacc acctgattgg gcgcacacat
      121 gcgtaaaggt cgaggatctg gcgaagagcg acgttcaagg atcggatccc accgcctacc
      181 ggggtcgtca acgggccctt gatcccaacc agataggtct caaatgcttc aaccgtctcg
      241 tcaggcagcc atgaaccgac ttcattgtgg gccttttccc cggccaacac ctcggtccag
      301 ttgaccttcc tcgagtcgcc atacgctttc ccaaccgcgg cgtcaaagac acgcaccgat
      361 gcggcccaga tgtcgggccc ggtgccgtcg ccttcgatga acgggacgat cgggtcgtcg
      421 ggaaccacca gcccttccgg gctcatggtg ataggggtgg ccatgaaagg gactcctgga
      481 gaagcggtta cggagccggg gacactagcc ggggccgcgg ttcggcaagt gggggaaggt
      541 cgatcagtcg gccaggccgg ccctcaccag gaacgcagcc atctctcccc tggtcacgtt
      601 gttgttggga cagaatcggg tgttggtggg cgggttgcat ccgagagtga tgccggcata
      661 ggcgagcctc gagatcgccg actcgaagat gtgcccccgg gtatcggtga agacccgcgg
      721 actgggtggc gccgggagct tgagagcgcg caccaggaag gcagccatct gccctcgtgt
      781 cactgggtcg tcagggcaga actttcgggg cccgcatccc tgggtgatcc ccgtcgcact
      841 cagccagttg atggagtccg agaagatcga agcgctcgag tcgtcgaaag ggttcgctgc
      901 attccgtgcg gcagcccagg tcttcacact cgacgagaaa agccagtcga cgccgccggt
      961 tcgagccccc tgtgtcgctg cggcgaagcc cgtctggagc cctgacgtag tgctcacccg
     1021 gaggtcgagt gatcccttga ccaggtcgtc ccattgcacg ccaacgattt gggggtgcga
     1081 caccgcaagg cttgccagag cctgtgcgta ctggacctgc gccgcctcat tggggagatt
     1141 cggagcggcg ccccctgaga tgaggatcgg gattcctggg cctacggcgt tcgccatctc
     1201 gtccacagcc gacttgtgat agccgacgtt cgatccaccc gtcgccgggt agcccccaac
     1261 cccggcggca tcggcatagg aggcaccggg ccagtagagg ccgaacccgc ccgtgcccca
     1321 ctgtgattgc gaatagcgac tcgatgacat cgaccggttg ccgctgaaca cgactctcac
     1381 ccgcgaagac ccgatctcgg ctcgcgcctg ggtggcgatg ttgcggaatg cagtcttgaa
     1441 cgacgaggga tcgtcacccc agggattatt ccggacattg ggctcgggaa ggatgtcgag
     1501 tatc
//
LOCUS       DBNHXU010000184         5853 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3431383, whole genome shotgun sequence.
ACCESSION   DBNHXU010000184 DBNHXU010000000
VERSION     DBNHXU010000184.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5853)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5853)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5853
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3431383"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1267)
                     /gene="prfB"
                     /locus_tag="AC161X_04620"
     CDS             complement(140..1267)
                     /gene="prfB"
                     /locus_tag="AC161X_04620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_153759895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016149 - translation release factor
                     activity, codon specific [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HIR4988864.1"
                     /translation="MESSDPKEIVADLRQRLVEARRFLDLDTKEKELDDLREEAAAPA
                     LWENPDRARTVSRRLARYEALFDLIGGLSAKLDDAEVLLDMAHEAEDPASRDEAIAEL
                     WEVSADLDKLELESLFFDEYDEEDAILSVHAGAGGVDAQDWADMLARMYQRFLTDSGF
                     SVTVEEVTEGEEAGIKSATFTVRGDRAYGTLESERGVHRLVRISPFDANARRQTSFAG
                     VDVVPDLGDQAKVEVDEDDIRIDTYRSQGAGGQHVNKTDSAVRITHIPTGIVVQCQNE
                     RSQLQNKARAMTMLQSKLAQLARQERIDHLNEIRGDKGEAAWGRQIRSYVMQPYQMVK
                     DLRTDEEVGNIQGVLDGDVAPFVEAYLRWRRANHEASTNST"
     gene            complement(1313..3922)
                     /gene="secA"
                     /locus_tag="AC161X_04625"
     CDS             complement(1313..3922)
                     /gene="secA"
                     /locus_tag="AC161X_04625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009612661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0017038 - protein import [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HIR4988865.1"
                     /translation="MPILSKVLRAGEGKTLREFQGITTAVNQLEPVFEALSDEELKAK
                     TPEFKKRVESGASLEDIEAEAFAVVREASKRVLGQRPYDVQVVGAAALHKGMVAEMKT
                     GEGKTLASSMPAYLNALNGKGVHMVTVNDYLASRDADWMGAIHRFLGLTVGLIQTSMS
                     PSERQPAYASDITYGTNNELGFDYLRDNMAMRAVDRVQRGHPYAIVDEVDSILIDEAR
                     TPLIISGRVSDSARWYRDFAKLANRLQKDRDYQVDERKRQVLTTEEGVTLVEQMLGVE
                     NLFDHAAVDFVHHLEAALKAKELYLHDVDYLIDHGEVKIVDEFTGRVLEGRRYSEGLH
                     QAIEAKEGVAVKEENQTLATITLQNYFRLYEKLAGMTGTAETEAAELAQIYNLEVIAI
                     PTNAPVDRADEGDLVYKGEDGKYDSVVADVVERNTLGQPVLLGTVSIEKSEKLSRLLE
                     KQGIKHEVLNAKQHAREADIIAQAGRPGAVTVATNMAGRGVDIMLGGNPEGMARTELK
                     RRGVSPDDPSYADLEKKLTEEFELQIVPDKERVIGAGGLYVVGTERHESRRIDNQLRG
                     RSGRQGDPGESRFFLSLEDDLMRRFQGEWVTGVMDRLKLPEDQPIEAKMVSKAIERAQ
                     RQVESQNFEIRKNVLKYDEVMNRQREVIYEWRRSILEGTAGDDLIGDWIESVVTETVE
                     TAISPEIPRNEWDWEALRLELSQYYPASIDRSSFEDSPDVDDVVEYAVDQGVKAYEQR
                     EQDLGSDVLRQVERSVMLSVIDNKWREHLSEMDYLRAGIGLRAMGQRDPLSEYQREAY
                     DMFTEMVDSVKREAVKYLFHVELSKPKSEPKRVEPLSTSGPVTQATSDKVGRNDPCPC
                     GSGKKYKRCHGAA"
     gene            complement(3975..4625)
                     /locus_tag="AC161X_04630"
     CDS             complement(3975..4625)
                     /locus_tag="AC161X_04630"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR4988866.1"
                     /translation="MSSVVIVDDAELFREALRAAFAQEGFDVRAVAADAMKGIDITRE
                     HQPDLVMLDLLMPGMSGLEVVGTIIKASPRTKVVLLTSSESAEDLLAAVKAGASGYLT
                     KDTPLPRLVAAMHDVIDGGAAVSPAMGGKLFTALRDLLKHSGMMSARVPELTGRELEI
                     LGLVGNGKTSREIAEELFISENTVRNHVRNVLDKLGLRSRFEAVTWAQREGLIQVR"
     gene            complement(4657..5241)
                     /locus_tag="AC161X_04635"
     CDS             complement(4657..5241)
                     /locus_tag="AC161X_04635"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HIR4988867.1"
                     /translation="MLCQSCFAATKGILCATCRGLMRPAPDRVLSGGVRLVAAFEHSG
                     PARALVHQLKYRGLTAYADVVAMVLDGVIPRLPIVPVPRVWSRQLQYGVDPAVVLAER
                     LARSLRVPVIKSLSAPIHTRRRAGGDHSRPIAPFRMTEVPAGGVVLVDDVVTTGRTLE
                     AAIGLLGAERVALAVCANAAPPVSSLPAPKRGQI"
     gene            complement(5300..>5853)
                     /locus_tag="AC161X_04640"
     CDS             complement(5300..>5853)
                     /locus_tag="AC161X_04640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015169620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HIR4988868.1"
                     /translation="VEVNPVRALTAAMDGYRVMTSDDAAREGDIFVTVTGNKHVLRSH
                     HFDMMKDGAILANAGHFDIEIELDALARKSVARREIRDNLEEFELADGRRLLVAAEGR
                     LVNLGAAEGHPADVMDMSFANQALAAEFLVLNQDELEPIIHTLPPEIDQEVARIKLEH
                     LGGGLETLTDEQIAYLSGWREGT"
BASE COUNT         1050 a         1849 c         1746 g         1208 t
ORIGIN      
        1 aatgagacgt cgtgggcagc aatggtgccg ccctcataga ccttggtgac gttttcgagt
       61 cggatcatgg gatcaggagc agtctcgcag gcatctgcct ccagccgcag aaccgaggac
      121 ggtaaccaga caccgccgac taggtggaat tagtcgatgc ctcatggttg gcgcgacgcc
      181 agcggagata ggcctcgaca aacggtgcca catccccatc caggacaccc tggatgttcc
      241 cgacctcttc gtcggtccgc aggtctttga ccatttgata cggctgcatc acgtagctcc
      301 ggatctggcg accccaggcg gcctcccctt tgtcgccgcg aatctcgttg agatgatcga
      361 ttctctcctg tcgggcgagt tgggcgagct tggactggag catggtcatg gctctcgcct
      421 tgttctggag ttgtgaccgc tcgttctggc attggacgac gatcccggtc gggatgtgcg
      481 tgatccgcac cgcggagtcc gtcttgttca cgtgctggcc accggcgcct tgggagcgat
      541 aggtatcgat tcgaatgtcg tcctcgtcga cttcgacttt tgcctggtct cccaggtcag
      601 ggaccacatc cacgcctgcg aacgaggtct gacgcctggc gttggcatcg aaggggctga
      661 tcctcaccag tcggtgaacc ccgcgctcac tctccagggt tccataagcg cggtcgcccc
      721 tgaccgtgaa ggtcgccgac ttgatccccg cctcttcgcc ctcggtcacc tcctcgaccg
      781 tcactgaaaa gccggaatca gtcaggaatc gctgatacat cctggccagc atgtcggccc
      841 agtcctgggc gtccactccc ccggctccgg cgtggacact gaggatggcg tcctcctcgt
      901 cgtactcgtc gaagaaaagt gattcgagct cgagcttgtc caggtcggcc gatacttccc
      961 agagctcggc aatcgcctcg tcgcgtgacg cgggatcttc agcctcatgg gccatgtcga
     1021 gcagcacctc ggcgtcgtcg agtttggctg aaaggccacc aatcagatcg aagagagcct
     1081 cataacgagc gagtcggcgg ctgacggtgc gggctcggtc aggattctcc cagagggctg
     1141 gagcggcggc ctcctcgcgt aggtcgtcga gctccttctc cttggtgtcg aggtccagga
     1201 atctgcgtgc ctcaaccaga cgctgccgca gatcggcgac gatctccttc ggatccgagg
     1261 attccatact catgctgtga acggttagcg gtgcacgatc aacgtgatgc cgctacgcgg
     1321 ccccgtgaca acgcttgtac ttcttgcccg agccacaggg acaaggatcg ttgcggccta
     1381 ccttgtccga tgtggcctga gtcaccgggc cgctggtgct caacggctcc accctcttcg
     1441 gctcgctttt cggcttcgag agctcgacgt ggaacaggta cttgacggcc tctcgcttca
     1501 ccgagtcgac catctcggtg aacatgtcgt aagcctcccg ctgatactcg gagagcgggt
     1561 ctcgctgacc catcgcccgc aatccgatcc ccgcgcggag gtagtccatc tcggagagat
     1621 gctcccgcca tttgttgtcg atgaccgaga gcatcacaga gcgctcgacc tgacggagga
     1681 cgtcggaacc gagatcctgc tcacgctgct cgtatgcctt cacaccctga tcgaccgcat
     1741 actccacgac gtcgtcgaca tcgggagagt cttcgaaact cgatcgatcg atcgaggccg
     1801 ggtagtactg gctgagctcc aggcgcagcg cctcccagtc ccactcgttg cgaggaatct
     1861 cggggctgat cgcagtctca acagtctcgg tgacgaccga ctcgatccag tcgccgatca
     1921 ggtcgtctcc agcagtgccc tcgaggatcg acctccgcca ctcgtagatc acctcacgct
     1981 gacgattcat gacctcgtcg tacttcagga cgttcttgcg gatctcgaag ttctgtgact
     2041 cgacctggcg ctgggcacgt tcgatcgcct tgctgaccat cttggcctcg atgggctggt
     2101 cctcgggcag cttcaagcga tccatgacac cggtgaccca ctccccctgg aagcgacgca
     2161 tcagatcgtc ttcgagggac agaaagaacc tggactctcc agggtcccct tggcggcccg
     2221 accgaccacg cagctgattg tcgatccgcc gagactcgtg tcgctcggtc cccacaacgt
     2281 aaagaccgcc ggcaccgatc actctctctt tgtcaggcac gatctggagc tcgaactcct
     2341 cggtcagttt cttctcgaga tcggcatacg aaggatcatc ggggctgacg ccacggcgtt
     2401 tcaattcggt ccgggccatg ccttcggggt ttccgccgag catgatgtca acaccgcgac
     2461 cagccatgtt ggtcgccact gtcaccgcac ccggacgacc tgcctgggcg atgatgtcgg
     2521 cttcccgagc atgctgcttg gcgttgagaa cctcgtgctt gattccttgc ttctcgagga
     2581 gccgcgacag cttctccgat ttctcgatgg agacggtgcc caaaaggacc ggctggccga
     2641 gggtgttgcg ctcgacgacg tcagccacca cagagtcgta cttgccgtcc tcgcccttgt
     2701 agacgaggtc gccctcgtcg gctcgatcga cgggagcgtt ggtcgggatg gcgatcactt
     2761 cgaggttgta gatctgggcg agctcggcgg cctcggtctc ggcggtcccg gtcatgcccg
     2821 ccagcttctc gtacagccgg aagtagttct ggagggtgat ggtggcgagg gtttggttct
     2881 cctccttgac cgcaacccct tccttggcct cgatcgcctg gtgaaggcct tctgagtagc
     2941 gccggccctc gaggacgcgg cccgtgaact catcgacgat cttcacttcg ccatggtcga
     3001 tcagatagtc gacatcatgg agatagagct ctttggcctt gagagcggct tccagatgat
     3061 ggacaaagtc aacagcggcg tggtcgaaga ggttctccac tccgagcatc tgctcaacca
     3121 gggtcacgcc ttcttcggtg gtgaggacct ggcgcttgcg ctcatcgacc tgatagtcgc
     3181 ggtccttctg gagacgattg gcgagcttgg cgaagtcccg ataccacctt gcactgtccg
     3241 agaccctgcc cgagatgatg agcggggttc gggcttcgtc gatgaggatc gagtccacct
     3301 cgtcgacgat tgcgtagggg tggccgcgct ggacgcggtc cacagctcgc atcgccatgt
     3361 tgtctcggag ataatcgaag ccgagctcgt tgttggtccc gtaagtgatg tccgaggcgt
     3421 atgcgggttg gcgctcagag gggctcatcg aggtttggat gaggcccacg gtcagtccca
     3481 ggaaccggtg gatggcaccc atccagtcgg cgtcacgtga tgccaggtag tcgttcaccg
     3541 tcaccatgtg cacgcccttg ccgttcagcg cgttgagata ggccggcatg ctcgaggcga
     3601 gtgtcttgcc ctcaccggtc ttcatctcgg caaccatccc cttgtggagt gcagcagcac
     3661 caaccacttg cacgtcatag ggacgctggc ccaggactcg cttggaggct tcacgaacca
     3721 cggcgaaggc ctcggcctcg atgtcttcga gggaggcgcc cgattcgacg cgcttcttga
     3781 attcaggtgt ctttgctttt agctcttcgt cggagagggc ttcgaagacc ggctccagct
     3841 ggttcacagc agtggtgatc ccctggaact ctctcagcgt cttgccctcc ccggcacgga
     3901 gaaccttgga gagaattggc attagagcct catgttacaa gtgggtgcga tttgacccac
     3961 ttcgcgagcc gcgatcaacg gacctggatc aggccttctc gctgggccca ggtcacagcc
     4021 tcgaatcggg agcgaaggcc gagcttgtcg aggacgttgc gaacgtgatt gcggacggtg
     4081 ttctccgaga tgaacaactc ctcggcgatc tcccgcgaag tctttccatt tccgacaagg
     4141 ccgaggatct cgagctccct tccggtgagc tcggggacac gggcactcat catgccgctg
     4201 tgcttgagca gatccctcaa tgcggtgaag agcttcccgc ccatggcagg tgatacggcg
     4261 gctccaccgt cgatcacgtc gtgcatggcg gcgaccagac gtggaagcgg ggtgtctttg
     4321 gtcagataac ccgatgcccc ggctttcacc gcggcgagta ggtcttccgc cgactcgctg
     4381 gaagtcagca ggacgacttt ggtgcggggg ctcgccttga tgatggttcc caccacttcg
     4441 agacctgaca tccccggcat caaaaggtcg agcatcacga ggtctggctg atgctccctg
     4501 gtgatgtcga tgcccttcat ggcgtctgcc gctacggctc tcacatcgaa gccctcttgt
     4561 gcgaaggcgg cacgcagcgc ctcgcggaag agctcggcgt cgtcgactat gactacggat
     4621 gacattcttg gtgttacctg gctattggca aaaagttcag atctgaccac gtttcggtgc
     4681 cgggagacta gacaccgggg gcgccgcatt ggcacaaacc gccaacgcca ctctctctgc
     4741 gccgagcaaa ccgatcgccg cttcgagagt cctcccggtg gtgaccacat cgtcgaccaa
     4801 cacaacgccg cccgcgggga cctcggtcat ccggaacggc gcaattggtc tcgagtggtc
     4861 gcctcctgca cgtctacggg tatgtatcgg cgccgaaagt gatttgataa ccggaaccct
     4921 gagcgatctc gcgagtcttt cggcgagcac gaccgccggg tcgacgccgt actgcaactg
     4981 acggctccac acacggggca caggaacgat ggggagcctg gggattacgc cgtcgaggac
     5041 catcgcgacc acgtcggcgt atgccgtgag accccgatat ttgagctgat gaaccaacgc
     5101 acgagccggt ccggagtgct cgaacgccgc caccaatcgg acgcccccgc tcagcacacg
     5161 atccggagcc gggcgcatca ggccccggca tgtcgcgcag agaattccct tggtcgccgc
     5221 aaagcaagac tgacagagca ctctctccga ccttacgaga cggacgaaaa gctttcgagg
     5281 cctccgtatt aactaggcct caggtgcctt cgcgccaccc cgacaggtat gcgatctgct
     5341 catccgtcaa ggtctcgaga cctccaccga gatgctccag cttgatgcgg gccacctcct
     5401 ggtcgatctc gggaggcaga gtgtggatga tcggttccaa ctcgtcctga ttgaggacga
     5461 ggaactcggc ggccagggcc tgattggcga aactcatgtc catcacatcg gcgggatgac
     5521 cttcggccgc gccgagattg accagacgac cctcggcggc cacgaggaga cggcgaccgt
     5581 ctgccaattc gaactcctcg aggttgtccc gaatctcccg tcgcgccact gacttccgag
     5641 ccagggcatc gagctcgatc tcgatgtcga aatggccggc attggccagg atcgccccgt
     5701 ctttcatcat gtcgaagtgg tggctccgca agacatgctt gttgccggtg acggttacga
     5761 atatgtcccc ttcccgggcg gcatcgtccg acgtcatgac tcggtagcca tccatcgccg
     5821 cggtcagcgc ccggaccggg ttgacctcga cca
//
LOCUS       DBNHXU010000185         4965 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4424278, whole genome shotgun sequence.
ACCESSION   DBNHXU010000185 DBNHXU010000000
VERSION     DBNHXU010000185.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4965)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4965)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4965
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4424278"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..912
                     /locus_tag="AC161X_04645"
     CDS             22..912
                     /locus_tag="AC161X_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR4988869.1"
                     /translation="MHLSMHNWMRAEPIEVTIARLAKFGYSSIEISGEPDIYDTRHVG
                     ALLEDAGLKCWGAVTLMLGERNLVAKDEAQRAASVKYVKDCITMVKELDGYEITIVPA
                     TVGKIIPDASPEEEWKWAVDGMSEIMAHARIEGIRPAIEPLNRFETYFINRGEQAMAL
                     AEATGPDCGVCLDAFHINIEEADWRQAIRDVGDRLTDFHVAENNRMPPGMGDYDWKEV
                     VSLLREVGYDGAMTMEFVAPIDRTPANHYPNAIEHNPQGISPEQLKFIQDHGSSTLSE
                     EFYDWMVMKASEHVLPLIQD"
     gene            915..2171
                     /locus_tag="AC161X_04650"
     CDS             915..2171
                     /locus_tag="AC161X_04650"
                     /inference="COORDINATES: protein motif:HMM:NF002674.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HIR4988870.1"
                     /translation="MVPNVVIVGTLDTKGPEVAYIRDRLIDRGCTTTVVDVGILGDPL
                     GIVPDVDHSAVAAYADANIDEVRQAGTRGRAVALMRQAVRSLMTDLYLQGKVDAARGL
                     GGAEGAVMGAAALMMLPIGVPKVVLSPIASGRHEFGPLVGTSDMMVVHTVVDIMGLNP
                     VATTVFDNVTTAVAAMAFDHSRLEPHGPDSRLVGVTMLGNTTKAVMALRDRLAEHGYE
                     AVVFHSNGVGGPAMEELAEAGLFVGIIDFTTNEVTDPMVGGIHDGGPRRLSTVAELGI
                     PQIVVPGCIDFSVFASGQVPLELADRPVYDHNPEYTLVRTPAGQMAQIGELFAERLNR
                     ATGPLAIAIPTNGLSIPNVPDGVFWDPDADAAFVKSLRRDLRPDIAIHEFDHHVNDPR
                     FGEVVADLFADLLDGAGITTRLEKAT"
     gene            2168..3076
                     /locus_tag="AC161X_04655"
     CDS             2168..3076
                     /locus_tag="AC161X_04655"
                     /EC_number="3.5.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018207146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HIR4988871.1"
                     /translation="MTTDTLADVTEAERAFREQFVSWNLAELSYVDIQAYLEDKDVVL
                     VPMASTEQHGPHLPLYCDTITAIEISARVSNLIGVLHTPPIWTGYSPQHMHRPGEGRG
                     TITVRSSTLLALMEDVARSLIHHGFNRIIFVNGHGSNIKVVDPVLRKLRYETGALIGL
                     VKPYMERYVGVVEGVFENPPEETPGWHSSEWETSQCLAWNEKLVRMDRAQHTPTSIPD
                     FLPESFRKYDGAPDVEFEGYTYFNFPMDHHEFTETGVIGNPMRATKEKGEESFRRYSE
                     HVARGVLELMKVPVKVENREFIDRVL"
     gene            3076..3771
                     /locus_tag="AC161X_04660"
     CDS             3076..3771
                     /locus_tag="AC161X_04660"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR4988872.1"
                     /translation="MAEPDPTTVTDRAYVQLRELLTSENVRPGDHLGEVAVATWLGIS
                     RTPVRAALQRLELDQLVRRNHNSGYSVAGLTAGDVHEICDVLRLLDTALFIRASENLT
                     DEQGANLQECVERMAMAASKRDLDLWSEGDARFHELLQDSSDHTLFSDIAEKQRRRLH
                     RFWMSGAGRLERLAKCADEHSSIAKSIAASDHDGIRRLVNEHIDHMEESMIRHLEMAR
                     PFISTNGSALDHV"
     gene            3764..>4965
                     /locus_tag="AC161X_04665"
     CDS             3764..>4965
                     /locus_tag="AC161X_04665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR4988873.1"
                     /translation="MSDPRADYRIRMLLHELGDVVGAKNVSVDPGDLAAQAADWSWMS
                     QLYRLHGIELPTADVMVRPGSSQEVADVVRVASDHRVPVVPRGGGSGTQGGTFALYGG
                     IALDLTRMDHILEVDETSLVVNVEAGVTGPQLEEALSPLGLAVAHEPGSFHFGATVGG
                     WLAARGSGVRSTKYGKPEDMVLQVEAALPPGVLASTPSVPNHAAGPGLLQLLVGSEGT
                     MGVITSASLRMDPLPETQAFLTYEFATVFDGLEAGRRIMTQRWHPSVMRLYDEADRDK
                     LNHILGLELTGALLVVICDGDQRLVDLESEVITGICSEVGGKYLGPGGAKTWWDGKYE
                     PYAEGKAPAPPTIFGTTDTVCTFDNMPDLYRAKRKNVEEGFAEYGAQYTAHFSHWFPW
                     GVMVYDRF"
BASE COUNT          975 a         1578 c         1535 g          877 t
ORIGIN      
        1 ctagtccgag gaggaccaac gatgcatttg tcgatgcaca actggatgcg agccgagccc
       61 atcgaggtga cgatcgcccg gctggcgaag ttcggatact ccagtatcga aatcagtgga
      121 gagcccgaca tctacgacac aagacacgtt ggagcgctcc tcgaagatgc cggcctcaag
      181 tgctggggag cggtgaccct gatgctcggt gagcgaaatc tggtcgccaa ggatgaagcc
      241 cagcgggcgg cttcggtcaa gtacgtgaag gattgcatca cgatggtcaa ggagctcgat
      301 ggctacgaga tcaccatcgt tccggccacc gtcggcaaga tcatccccga cgcctccccc
      361 gaggaggagt ggaagtgggc ggtcgacggg atgtccgaga tcatggctca cgcccgcatc
      421 gagggcatcc gcccggcgat cgaaccgctc aaccgcttcg agacttactt catcaaccgc
      481 ggcgaacagg ccatggcgct cgctgaggcg acaggtcccg actgcggggt gtgtctggat
      541 gcgttccaca tcaacatcga ggaagccgac tggcgtcagg caatccgtga cgtaggggac
      601 cgtctcactg actttcatgt ggccgagaac aatcggatgc ccccgggcat gggcgactac
      661 gactggaagg aagtcgtgtc gctcttgagg gaggtcggat acgacggcgc catgacgatg
      721 gagttcgtgg ctccgatcga ccgcaccccg gccaaccact atccgaatgc gatcgagcac
      781 aacccccagg gcatctcccc cgagcagctc aagttcatcc aggatcatgg gagctcgacg
      841 ttgagcgagg agttctacga ctggatggtc atgaaggcga gcgagcacgt gctgcctttg
      901 atccaagact gaggatggtc cccaacgtcg tcatcgtcgg caccctcgac accaagggcc
      961 cggaagttgc gtacatccgc gatcgtttga tcgatcgcgg gtgtaccacg acggtggtcg
     1021 acgtcgggat tctcggagat ccactcggca tcgtccccga tgtcgatcac agcgcggtcg
     1081 ctgcctatgc cgacgcgaac atcgacgagg tccgtcaagc gggaacccgg gggcgtgccg
     1141 ttgccttgat gcgtcaagcc gtgcggtcgc tgatgaccga cctctatctg caggggaaag
     1201 tcgacgcggc gagaggtctc ggcggagccg aaggggcagt catgggagcg gctgcgctga
     1261 tgatgctgcc gatcggggtc cccaaagtgg ttctcagccc aatcgcctcc ggccgtcacg
     1321 agttcgggcc tctggttggg acctcagaca tgatggtcgt gcacaccgtg gtcgacatca
     1381 tggggctcaa cccggtggcc acgaccgtgt ttgacaatgt caccaccgcg gtggcggcga
     1441 tggccttcga ccacagccga ctcgagcccc acggccccga cagccgcctg gtcggtgtca
     1501 ccatgctggg caataccacc aaggcggtca tggccctccg cgaccgattg gccgagcacg
     1561 gctatgaggc ggttgtcttt cattcaaacg gggttggtgg accggcaatg gaggaattgg
     1621 ccgaggccgg tctgttcgtg gggataatcg acttcacgac caacgaggtg accgacccca
     1681 tggtcggtgg gatccacgac ggcgggcccc gccggctgtc gacggtggcg gagctcggga
     1741 ttccgcaaat cgtggtcccc ggctgcatcg acttcagtgt cttcgccagt ggccaggtcc
     1801 ctttggaact tgccgaccgc cctgtctacg accacaaccc ggagtacacg ctggtccgca
     1861 caccagcggg ccaaatggcc cagatcggag agctcttcgc cgagcgattg aaccgggcaa
     1921 ccggcccact cgccatcgcc atccccacca atgggctgtc gatacccaac gtgccggacg
     1981 gcgtcttctg ggatcccgac gccgatgcag cctttgtcaa gagcctgagg cgcgatctcc
     2041 ggccggacat cgcgatccat gagttcgacc atcacgtcaa cgatcctcga tttggagagg
     2101 tcgtcgccga cctgttcgcc gacctcctcg acggggccgg cataacgacc cgactggaga
     2161 aagcaacatg accactgaca cattggcgga cgtcaccgaa gccgagcggg cgtttcgcga
     2221 gcagttcgtt tcctggaacc tggccgagct ctcgtacgtg gacatccagg cctacctcga
     2281 ggacaaggac gtggtcctgg tgccaatggc gagcaccgag cagcacgggc ctcatctgcc
     2341 cctctactgc gacaccatca ccgccattga gatatcggcc cgggtctcca acctgatcgg
     2401 cgtcctccac accccgccga tctggaccgg ctactcgccc caacacatgc atcgacccgg
     2461 agaagggcga ggcacgatca cagtccgttc gtcgaccctc ctcgccctta tggaggatgt
     2521 cgcccgaagc ctcattcatc acggcttcaa ccggatcatt ttcgtcaatg ggcatggttc
     2581 gaatatcaag gtcgtcgacc ctgttttgcg aaaacttcgc tatgagaccg gagccctgat
     2641 cggcttggtc aagccctaca tggagcgtta tgtgggcgtg gtggagggtg tcttcgagaa
     2701 tccccctgag gagacgccgg ggtggcattc gagcgaatgg gagacgtcgc aatgtctggc
     2761 ctggaacgaa aaactcgttc gcatggaccg ggcccagcac actccaacgt cgattccgga
     2821 cttcctgccc gagtcgttca gaaagtacga cggtgccccc gatgtggagt tcgagggcta
     2881 cacctacttc aacttcccga tggaccatca cgagttcacc gagacgggcg tgatcggcaa
     2941 cccgatgcgg gcgaccaagg agaagggcga ggaatcgttc cggaggtatt cagagcacgt
     3001 ggcacggggc gtgctggaac tgatgaaggt accggtcaag gtcgagaacc gagagttcat
     3061 cgaccgagtc ttgtgatggc agagcccgat cccacgaccg tcaccgacag ggcgtacgtc
     3121 cagctgcgcg agctgctgac aagcgagaac gtccgacccg gcgaccacct gggcgaggtg
     3181 gctgtcgcca catggctcgg tatcagccgg acaccggtga gggctgcgtt acagcgactc
     3241 gaactcgacc aactcgtgcg ccgcaaccac aacagtggct actcggtggc cgggctgacg
     3301 gccggcgacg tccacgagat ctgcgacgtc ctccgtctcc tcgacacggc cctcttcatc
     3361 cgagcgtcgg agaacctcac cgacgagcaa ggcgccaacc tccaagagtg cgtcgagaga
     3421 atggcgatgg ccgcctcgaa gcgagacctc gatctctggt ccgaaggcga cgcccggttc
     3481 cacgagctcc tccaggactc atcggaccac accctcttct ccgacatagc cgagaaacag
     3541 cgaagacggc ttcaccggtt ttggatgtcg ggggccggcc gcctcgagcg cctggcaaaa
     3601 tgcgccgacg agcactcatc gatagccaag tcgatcgccg cctccgatca tgacggcata
     3661 cgaagactcg tcaacgagca catcgaccac atggaagaga gcatgatccg ccatctcgag
     3721 atggcgcggc ccttcatatc caccaacgga tcagccctcg accatgtctg atccccgcgc
     3781 tgactaccgg atccggatgc tcctccatga gctcggggat gtggtcggtg ccaagaacgt
     3841 ctcggtcgac cccggggacc tggctgccca ggcagcagat tggtcgtgga tgtcccagct
     3901 ctaccgactc cacggcatcg agttgcccac ggcagatgta atggttcgac ccggaagctc
     3961 acaagaggtg gccgatgtgg tccgggtcgc cagtgaccat cgggtcccgg tggtgccgcg
     4021 tggcggcggg tcgggcaccc aaggtgggac gtttgccctg tacgggggaa ttgcgctcga
     4081 cctgacccgg atggaccaca tcctcgaagt ggatgagacc agcttggtcg tcaatgtcga
     4141 ggccggcgtg accgggcccc aactcgagga ggcgctgagc ccccttggcc ttgccgtcgc
     4201 ccacgagccc ggctcattcc actttggcgc caccgtcggc ggctggttgg ccgctcgagg
     4261 gtctggcgtc cggtcgacca agtacggcaa acccgaggac atggtccttc aagtcgaggc
     4321 tgccctgccc cccggcgtgt tggcgtccac gccctcggta cccaatcacg cggcaggccc
     4381 agggctgctt caactcctgg tcggctccga gggcaccatg ggcgtcatca cctcggcctc
     4441 gctccgcatg gaccctttgc ccgagaccca ggcattcctc acctacgagt ttgcgacggt
     4501 cttcgatggt ctcgaagcgg gtcgacgaat catgactcag cgctggcacc cctcggtgat
     4561 gcgcctctat gacgaagccg atcgggacaa gctgaaccac atcctggggc tcgaactgac
     4621 cggggcattg ctggtcgtca tttgtgacgg ggatcagcgt ctcgttgacc tcgaatcaga
     4681 ggtgatcaca gggatctgca gcgaggtcgg cggaaagtat ctcggtcccg gtggcgccaa
     4741 gacctggtgg gatggcaaat acgagcccta cgctgaaggc aaggcacccg ctcccccgac
     4801 catcttcggc accaccgaca cggtttgcac cttcgacaac atgcccgacc tctatcgggc
     4861 gaagcgcaag aatgtcgagg agggcttcgc cgagtacgga gcccagtaca ccgcacactt
     4921 ctcccactgg ttcccttggg gagtgatggt ctatgaccgc ttcta
//
LOCUS       DBNHXU010000186         1505 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1844503, whole genome shotgun sequence.
ACCESSION   DBNHXU010000186 DBNHXU010000000
VERSION     DBNHXU010000186.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1505)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1505
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1844503"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="AC161X_04670"
     CDS             complement(<1..379)
                     /locus_tag="AC161X_04670"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR4988874.1"
                     /translation="MTTRSTRAEPLAPDDRRRAIIAAVIPLLTEHGSTVTTKQMAEAA
                     GIAEGTIFRVFPDKHALIHEAVRFSMDPAPVVRALSEIDQSTPFEVQLTAAARIIFER
                     FQGVIALITALRTSPSPDAAPPAG"
     gene            complement(421..771)
                     /locus_tag="AC161X_04675"
     CDS             complement(421..771)
                     /locus_tag="AC161X_04675"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR4988875.1"
                     /translation="MSRPTDDELARHRTIDLTTLGRRSGRPSRIEIWWFYIDGSFFIT
                     GTPGPRDWYANVLADPNVTIHAGEHDLPATAQLVTDVETRSKVFDSELTRWYSSQSQR
                     QRLIDESPMVEIVF"
     gene            complement(755..937)
                     /locus_tag="AC161X_04680"
     CDS             complement(755..937)
                     /locus_tag="AC161X_04680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988876.1"
                     /translation="MGAPPESITCVECGGIAHLISFLPPDDDLEPGTPLAYRCSDCLD
                     RFDVVWEEGDGDVTPD"
     gene            complement(939..>1505)
                     /locus_tag="AC161X_04685"
     CDS             complement(939..>1505)
                     /locus_tag="AC161X_04685"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013421415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HIR4988877.1"
                     /translation="DVVLELEIGIVGGVEDASDNENIDRGKMYTSPEDMVRVAEVLGT
                     SEGGRYLLAAVFGNVHGVYKPGAVKLTPVILRDGQAAVEARFGESSRHYLVFHGGSGS
                     TLDEIRETLGYGVVKMNIDTDCQYAYTRPIVDHVMTNYAGVLKVDGNVGDKKAYDPRA
                     YMKKAESGMAARVVEACEQLQSAGKTIG"
BASE COUNT          258 a          458 c          496 g          293 t
ORIGIN      
        1 gcccggctgg cggagcggca tcaggagagg gtgaggtccg aagggcggtg atcagggcga
       61 tgaccccctg gaatcgttcg aagatgatgc gggccgctgc cgtcaattga acctcgaagg
      121 gggtggattg gtcgatctcg gagagagccc ggactaccgg ggcggggtcc atgctgaacc
      181 ggaccgcctc gtggatcagc gcgtgcttgt cggggaaaac ccggaagatg gtgccttcgg
      241 cgatcccagc cgcctcggcc atctgcttgg tggtcactgt cgacccgtgc tcggttagaa
      301 gagggatgac cgcagcgatg atggcccgac gacgatcgtc gggagccagc ggctcggcac
      361 gggtggatct ggtggtcacg gtcgcagtgt aactgagtga gtactcactc acaactgtaa
      421 ttagaaaact atctccacca tgggtgactc atcgatcagc cgttgtcgtt gcgactggct
      481 ggagtaccaa cgggtcagct ccgagtcgaa gaccttggac cgggtctcga catctgtgac
      541 cagttgggcg gtggccggga ggtcatgttc tccggcgtgg atggtcacgt ttggatcggc
      601 caggacgttg gcgtaccagt ccctgggccc gggggttccg gtgatgaaga agctgccgtc
      661 gatgtagaac caccagatct cgatccggct cggtctcccc gagcggcgac cgagggtggt
      721 caggtcgatc gtccgatgtc gggccagttc gtcgtcagtc gggcgtgaca tcgccatctc
      781 cctcttccca gaccacatcg aaacggtcca ggcaatccga gcagcggtag gcgaggggag
      841 tccccggctc caggtcgtcg tcgggtggga ggaaggagat caggtgggcg atgccgccac
      901 actcgacaca ggtgatcgac tctggtgggg cgcccaattc agccgatcgt cttccccgcc
      961 gactgaagct gctcgcaggc ctcgacgact ctcgccgcca tcccgctctc tgccttcttc
     1021 atgtaggccc gggggtcgta ggccttcttg tcgccgacgt tgccgtcgac cttgaggacg
     1081 cccgcgtaat tggtcatcac gtgatcgacg atggggcggg tgtaggcgta ctggcaatcg
     1141 gtgtcgatat tcatcttgac caccccgtag ccaagggtct cccggatctc atccagggtc
     1201 gagccgctgc caccgtggaa gaccaggtag tggcggctgc tctcaccgaa tctcgcttcg
     1261 accgccgcct gcccatctcg aagtatcacc ggggtcagct tgaccgctcc tggcttgtag
     1321 acgccgtgga cgttcccgaa gacggcagcg aggaggtagc ggccgccctc tgacgtgccg
     1381 aggacctcgg cgacccgcac catgtcctct ggcgaggtgt acatcttccc ccggtcgatg
     1441 ttctcgttgt cactggcgtc ctcgacgcca ccgacgatcc cgatctccaa ctcgaggacc
     1501 acatc
//
LOCUS       DBNHXU010000187         6124 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4820833, whole genome shotgun sequence.
ACCESSION   DBNHXU010000187 DBNHXU010000000
VERSION     DBNHXU010000187.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6124)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6124)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6124
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4820833"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..141
                     /gene="nadA"
                     /locus_tag="AC161X_04690"
     CDS             7..141
                     /gene="nadA"
                     /locus_tag="AC161X_04690"
                     /EC_number="2.5.1.72"
                     /inference="COORDINATES: protein motif:HMM:NF014498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008987 - quinolinate synthetase A
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinolinate synthase NadA"
                     /protein_id="HIR4988878.1"
                     /translation="MKRITLENIRDSLVTLTHQIEIDPAIAERARAAVSRMLEVGRQD
                     "
     gene            151..1665
                     /locus_tag="AC161X_04695"
     CDS             151..1665
                     /locus_tag="AC161X_04695"
                     /EC_number="1.4.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008734 - L-aspartate oxidase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-aspartate oxidase"
                     /protein_id="HIR4988879.1"
                     /translation="MTDITRLDAPLVVGAGVAGLTVALGLPRAYVMSAPEMGSTWWAQ
                     GGIAVALSEEDSPQQHAEDTVNVSGGLAVRNVVDVLTKGGPAAIENLIRLGAEFDRDD
                     DGGLSLGQEGGHLRRRVVHADGDATGAEVMRALTAAVVATDRIEIIERRVVDLVMADN
                     RVAGVITADGKNREVVLAPAVVLATGGAGRLYSRTTNPPGVTGDGIAMAGRVGARLAD
                     LEFVQFHPTALLAGKDPMPLLTEALRGEGAILVDAHGRRFMDQYHPMAELAPRDIVAR
                     AIFWQYDKGMGAYLDARSLVNFADRFPTVNAHAHSVGLDPEQDLLPVSPAAHYYMGGI
                     DTDSSGRTSIQGLWAVGECSSSGVHGANRLASNSLLEGLVFGARASDDVGRNLVEPDG
                     EPIAPKEAFDLPLVAGPALEELRQIMWDRVGLVRTGAGLWEARNHLLELENVLRRTIA
                     GRVAADLSLMVVLAALRRSESRGGHYRADYPEPDPMQEHRTLIDPTRADLVAVT"
     gene            1662..2501
                     /gene="nadC"
                     /locus_tag="AC161X_04700"
     CDS             1662..2501
                     /gene="nadC"
                     /locus_tag="AC161X_04700"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014189532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD+ biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HIR4988880.1"
                     /translation="MIDPAAYTDLVGRALAEDLGVAGDLTTDACIPEEAEAHAELVAR
                     GGGILAGLEVAEYVFHAIDPATDFAPMASDGAQVFAGQTVAAVAGLARSLLRAERTAL
                     NILCRMSGVATTTARFVAAVEGTGARITDTRKTMPGMRLLDKYSVSLGGGINHRLGLY
                     DAVLIKDNHIAAIGGIESAVTAARDRVGPDVTIEVEVETLDQLKVVLGTDADRVLLDN
                     MGVETLKLAVEMTDGRLVTEASGGVTLENVRSIALTGVDIISVGWITHSAPQMDLALD
                     FLT"
     gene            2528..3517
                     /locus_tag="AC161X_04705"
     CDS             2528..3517
                     /locus_tag="AC161X_04705"
                     /EC_number="1.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007924481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016615 - malate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006108 - malate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate dehydrogenase"
                     /protein_id="HIR4988881.1"
                     /translation="MSKQPVRVAVTGAAGQIGYSLLFRVAAGEMLGQDQPISLQMLEI
                     TPALGALEGVAMELDDCAFPLLAGVTMTDDPEQAFEDADVALLVGAMPRKAGMERSDL
                     LSANGAIFTVQGRALAAAAKPDVRVLVVGNPANTNALIAMNSAEGLDPRRFTAMTRLD
                     HNRAISQLAIRVDRTVESVKKMTIWGNHSSTQYPDLFNCEVDGAQAYQLVGDHGWVNE
                     VFIPSVAGRGAAIIAARGASSAGSAANAAISHIRDWVLGTPDGDWVSMGVPSDGSYGV
                     PEGLVSSFPCVCRDGEWGIVTGLEVDDYSRSKIEASVAELIEERDAVTALGLI"
     gene            complement(3527..4057)
                     /locus_tag="AC161X_04710"
     CDS             complement(3527..4057)
                     /locus_tag="AC161X_04710"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR4988882.1"
                     /translation="MRRDRGVAAIEFAFSFMILLMLALGAYEWGTGFADRISMTSAVR
                     EGGRVGSAAGDNPDADCRIIEAAAGALNGSTGNSVKQLWVYQSDAAGTEGLNRQIYRP
                     ATAAEPGLTCTGGKWFLIANGWPSDNRENLGANRDWLGVKVIMDHQWRTGFLWWNGSV
                     QWEEDAVFHLEPGVVT"
     gene            complement(4054..5466)
                     /locus_tag="AC161X_04715"
     CDS             complement(4054..5466)
                     /locus_tag="AC161X_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HIR4988883.1"
                     /translation="MKRWKQDEGIALVWLAITLVLLIGMAGFGIDLGWIYLNTARAQK
                     TVDSAALAGVVNLPGFIAQATIDAEDATVANGFDPDGLVVTPLADNKLHAELSITVPS
                     FFLKVLGFDHFDITREATAEYIKPVPLGNPNNCFGCPSVGDSWAAISAPFIHKEHGDP
                     YSTQCRVPATNSSCSTGNSDFNRGGAYSGYYYGIEVFGTSTTLSVDLYDPTFNRRPQD
                     QGTGDFSIGNGTGPTTNYELHMVDNTPLDPTDNPLVGGCSASFAPDTGANQDQWVNLC
                     TINGSLTPGIYVLHVTTTGVGIASNHYSVRANVGSGPQQPRVYGINDMSIWSNDLVQN
                     SQLYIAEIDDIHAGKKLELQFYDPGDADQNSFMTLLSPNGPVNCSWTVWNHNLTAQTQ
                     AAHSNQPCTWQTTDENKNPDHRVYNEQWIVAVIDLPDDPADMCNGSDCFWKMELDLSL
                     PTERTTWRARVIGNPVRLIP"
     gene            complement(5463..6008)
                     /locus_tag="AC161X_04720"
     CDS             complement(5463..6008)
                     /locus_tag="AC161X_04720"
                     /inference="COORDINATES: protein motif:HMM:NF019428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HIR4988884.1"
                     /translation="MGKGDKNRGATMVEAALIFPLMILLTVTLLELGLAFKDFLTVSF
                     TARDAARVGALAGNDPEADCHIVQSIVAGFSASNLSGMTISIFKANEATGNPVVGAVN
                     TWSLKPSGDPSTCAALDWNISEQWPSTSRNVTVGPTTKLDILGVTLDTTHNWITGLPP
                     WRGTINVSRTALQRIEPEAFE"
BASE COUNT         1078 a         1829 c         2025 g         1192 t
ORIGIN      
        1 cctcacatga agcggatcac actcgagaac atcagggatt cgctggtcac cctgacccac
       61 cagatcgaga tcgatcctgc aatcgccgag cgagcccgtg ccgccgtctc ccgaatgctc
      121 gaagtcggcc gccaggacta gaactatcca atgacggata tcacgcgcct cgacgccccc
      181 cttgtcgtcg gcgccggcgt ggccggactg accgtggcgc tcgggcttcc ccgtgcctac
      241 gtcatgtcgg cgccggagat gggctccacc tggtgggccc aggggggtat cgcagtagcc
      301 ctttcggaag aggattcccc acagcaacac gccgaggaca ccgtcaacgt atccggtggc
      361 cttgcagtca ggaatgtcgt cgacgtcctc accaagggag gcccggcggc gattgagaac
      421 ctgatccgac tcggtgccga gttcgaccgg gatgatgatg gcggcctctc acttggacag
      481 gaaggagggc acctgcggag aagggtggtc cacgccgacg gggatgccac cggggccgag
      541 gtgatgcgag ccctgactgc ggctgtggta gccaccgatc gcatcgagat aatcgagcgc
      601 agggtcgtgg atttggtgat ggcggacaat cgtgtggccg gcgtgattac tgccgacggg
      661 aagaatcgtg aggtggtcct ggctccagcg gtggtgctcg ccaccggcgg tgcgggccgt
      721 ctctattcac ggaccacgaa cccaccgggc gtgacgggag acggcatcgc catggcaggc
      781 cgggtcggcg cacgactggc cgatctcgaa ttcgtccagt tccaccccac tgcgttgctg
      841 gcgggcaagg acccgatgcc gctgctaacc gaagccttgc ggggtgaagg ggcgatcctg
      901 gtcgatgccc acggacgccg gttcatggac caataccacc cgatggcgga gctcgcccca
      961 cgggacatcg tcgccagggc catcttctgg caatacgaca agggtatggg cgcctatctc
     1021 gatgctcgct cgctggtgaa ctttgccgat cgatttccaa cggtcaacgc tcacgcccac
     1081 tcggtggggc ttgacccgga gcaggacctt ctcccggtct cgccggcagc ccactattac
     1141 atgggtggca ttgacacaga ttccagtggt cggacatcga tccagggctt gtgggccgtg
     1201 ggggagtgca gttcgagtgg tgtccacggg gcgaaccggt tggcctccaa ctcattgctc
     1261 gaaggccttg tgtttggggc gagagcgtcg gacgatgttg gccgcaacct ggtcgagccc
     1321 gatggtgagc caatagcgcc caaagaggcg ttcgacctgc cgttggtggc cgggccggca
     1381 cttgaggaat tgcgccagat catgtgggat cgggtaggac tggtgcggac tggtgccggg
     1441 ctttgggagg cacgcaatca ccttctggaa ttggagaacg tccttcggcg gacaatcgcc
     1501 ggccgggtgg cggcggatct ctcgttgatg gtggtgcttg cggccctgcg cagaagtgag
     1561 tcccgaggcg ggcactatcg ggccgactac cccgagccgg acccgatgca ggagcatcgg
     1621 acgctcatcg acccgacccg ggccgacctc gtggcggtca cgtgatcgat cctgctgcat
     1681 acaccgacct tgtggggcgt gcattggccg aggacctcgg tgtggcgggc gacctcacca
     1741 ccgatgcctg cattcccgag gaggcggagg cccacgcaga gcttgtcgct cgtggagggg
     1801 ggatccttgc cggtctggag gtcgccgaat atgtgttcca tgcaattgat ccggcgacgg
     1861 attttgcgcc gatggcgtcg gatggggctc aggtgttcgc gggtcagacc gtcgccgcgg
     1921 tcgccggcct ggccagatcc ctacttcgag ctgagagaac ggcgctgaac atcctgtgcc
     1981 ggatgtccgg tgttgccacc accaccgcac gatttgtcgc tgctgtcgag ggaactggcg
     2041 ctcggatcac cgatacccgc aagacgatgc ctgggatgag gctgctcgac aagtactcgg
     2101 tctcgttggg aggcggcatc aaccatcgcc tcggcctcta cgacgcagtg ttgatcaagg
     2161 acaaccacat cgccgccatt ggcggcatcg aatcggctgt gacggcggcg cgggatcggg
     2221 ttgggcccga cgtcaccatc gaggtcgagg tcgagaccct cgatcagctc aaagttgtgc
     2281 tgggcaccga cgccgatcgg gtgctcctcg acaacatggg tgtcgagacg ctgaagcttg
     2341 ctgtcgagat gacggatggc cggctggtca ccgaggcgtc tggaggggtg accctcgaaa
     2401 acgtccggtc gatcgccctg accggggtcg acatcatctc cgtgggttgg atcacccact
     2461 ccgcccctca gatggacctt gccctcgatt tcctgacgtg acgcctcgcg cgtaatttgg
     2521 ccaccacatg agcaaacaac cagttcgcgt tgccgtcacc ggtgccgccg gccagatcgg
     2581 gtacagcctt ctgttcaggg tggccgctgg tgagatgctc ggccaagacc agccgatcag
     2641 ccttcagatg ctcgaaatca cgccggcact tggcgctctc gagggcgtcg cgatggagct
     2701 cgacgactgc gcatttcccc tcctcgccgg ggtgacgatg accgatgacc cggagcaagc
     2761 attcgaggat gcggacgtcg ccctcctcgt cggggccatg ccccgcaagg ctgggatgga
     2821 gcgctccgac ctgctttcag ccaacggggc gatcttcacc gttcaggggc gtgccctggc
     2881 ggcggcagcg aagcccgatg tcagagttct ggtggtgggg aacccggcga acaccaacgc
     2941 tctgatcgca atgaacagcg ccgagggcct cgacccgagg agattcaccg ccatgacgcg
     3001 tctggaccac aaccgggcga tcagtcagct ggcgatacgg gtcgatcgca ccgtcgagtc
     3061 ggtgaagaag atgacgatct gggggaacca ctcttcgacc cagtatccgg acctattcaa
     3121 ttgtgaggtc gacggcgccc aggcctatca gctcgttgga gaccacggtt gggtgaatga
     3181 ggtcttcata ccgagcgtgg ccggtcgggg agcggcgatc atcgccgccc ggggagcttc
     3241 gtcggccggt tcggcggcca acgccgctat ctcgcacatt cgtgactggg tgttggggac
     3301 tccagatggc gactgggtct ctatgggtgt cccgtccgac ggttcctacg gagtgcccga
     3361 aggcctggtt tcctcgttcc cgtgcgtctg tcgggatggc gaatggggaa tcgtgaccgg
     3421 gctcgaggtc gacgactact cgagatccaa aatcgaggcg tccgtggccg agctcatcga
     3481 agagcgggat gcagtcaccg cgctgggttt gatctgagga ctggcctcag gtcactactc
     3541 caggctcgag gtggaagacg gcgtcctctt cccactgcac cgagccattc caccagagga
     3601 atccggtccg ccattggtga tccatgatca ccttgacccc gagccaatcc ctgttggcac
     3661 cgaggttctc cctattgtcc gagggccaac cgttggcgat caggaaccac ttcccgccgg
     3721 tgcaggtcag gcccggctca gccgcggtgg ctggccgata gatctgacga ttgagacctt
     3781 cggtccccgc agcatcggac tggtaaaccc atagctgctt gacggaattg ccggtggagc
     3841 cgttcagagc gcccgcagcg gcctcgatga tgcgacagtc ggcgtcgggg ttgtccccag
     3901 cagcgctgcc gacccgcccc ccttcccgga cggcagaggt cattgagatc cgatccgcga
     3961 atccggtgcc ccactcatag gctccgagcg cgagcatcaa caggatcatg aaggagaagg
     4021 cgaattcgat ggcggccact ccgcggtctc ttctcatggg atcaaccgca ccgggttacc
     4081 gatgacccgg gccctccagg tggtgcgctc ggtaggcaga ctcagatcca actccatctt
     4141 ccagaagcag tccgagccgt tgcacatgtc cgcaggatcg tcggggaggt cgatgacggc
     4201 cacgatccat tgctcgttgt aaacgcgatg atcggggttc ttgttctcgt ccgtcgtctg
     4261 ccatgtgcag ggctgattgc tgtgagccgc ctgggtctgg gccgtgagat tgtggttcca
     4321 caccgtccaa ctgcagttga ccggcccgtt gggggagagc agggtcatga acgagttttg
     4381 gtcggcatcg cccgggtcgt agaactggag ttcgagcttc ttgcctgcgt ggatatcgtc
     4441 gatctcggcg atgtacaact ggctgttctg gaccaagtcg ttggaccaga tcgacatgtc
     4501 gttgatccca tagacacgag gctgttgtgg gccactgcca acgttggctc ggactgagta
     4561 gtgattgctg gctatgccca ctccggtggt ggttacgtgg aggacataga tgcccggggt
     4621 cagcgagcca ttgatggtgc agagatttac ccactgatcc tggttggcgc cggtgtccgg
     4681 ggcaaaagag gccgagcagc caccgaccag cgggttgtcg gtggggtcga gcggagtgtt
     4741 gtccaccatg tgtagctcgt agttggtggt ggggccggtg ccgttgccaa tcgagaagtc
     4801 tccggtcccc tgatcctggg gtcttcgatt gaatgttggg tcgtaaaggt cgacagacag
     4861 agtggtcgaa gtgccgaaga cctcgattcc gtagtagtac cccgagtagg caccgccccg
     4921 gttgaagtca ctgttccccg tcgaacagga cgagttggtg gccggcaccc ggcactgggt
     4981 ggaatagggg tcgccgtgtt ccttgtgaat gaacggggcc gagatcgcag cccagctgtc
     5041 gccgacactg gggcacccga agcagttgtt gggattgccc aggggcaccg gtttgatgta
     5101 ttcggcggtg gcttctcttg tgatgtcgaa gtggtcgaag ccgagcacct ttaggaagaa
     5161 ggacgggacc gtgatgctca actcggcgtg cagcttgttg tccgccagcg gggtgaccac
     5221 gagcccgtcc gggtcgaagc cgttggcaac cgtggcgtct tcggcatcaa tggtggcctg
     5281 ggcaatgaat ccgggaaggt tgactacacc agccagggcg gccgaatcga ctgtcttctg
     5341 ggcccgagcg gtgttcaggt agatccagcc caggtcgatt ccgaaacccg ccattccgat
     5401 caggaggacc agcgtgatgg cgagccaaac cagggcaatg ccctcgtctt gcttccaccg
     5461 tttcattcga atgcctccgg ttcgattcgc tggagagcgg ttcggcttac gttgatcgtt
     5521 ccccgccatg ggggcaagcc ggtgatccag ttgtgggtgg tgtcgagggt gacgcccagg
     5581 atgtccagct tggtggtcgg gccgaccgtc acgttgcggc tggtgctggg ccactgctcc
     5641 gagatgttcc aatcgagagc agcgcacgtg cttgggtcgc ctgacggctt caggctccag
     5701 gtgttcacgg ctcccaccac ggggttgccg gtggcctcgt tcgctttgaa gatgctgatg
     5761 gtcatgcccg aaagatttga tgcactgaat ccggccacga tgctctggac gatgtggcag
     5821 tcggcttccg gatcattccc ggcgagcgcc ccgacccggg ccgcgtcacg tgcggtgaac
     5881 gagaccgtga gaaagtcctt gaatgcgagc ccaagctcga gaagggtcac cgtcaacagg
     5941 atcatcaacg ggaagatcag ggcggcctca accatggtcg cgccgcggtt cttgtctccc
     6001 ttgcccattt ttgcccctga cgtggccacc agccgtggtc actgacacaa cattatccga
     6061 cccacgccgc ttggtatcca gggaccccct cgtttttacg cgaactgact agtcggtgtc
     6121 agta
//
LOCUS       DBNHXU010000188         8474 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_653583, whole genome shotgun sequence.
ACCESSION   DBNHXU010000188 DBNHXU010000000
VERSION     DBNHXU010000188.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_653583"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="AC161X_04725"
     CDS             complement(<1..293)
                     /locus_tag="AC161X_04725"
                     /inference="COORDINATES: protein motif:HMM:NF012334.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR4988885.1"
                     /translation="MENGNVIVVGGTSGLGLEVARHYAAGGSQVVITGSDPGRTGEIA
                     STIEGDVTGIGFDLTDPSAIAPALHEVGTVSRLVLAAIARDNNFAKEYDIDAGT"
     gene            408..1304
                     /locus_tag="AC161X_04730"
     CDS             408..1304
                     /locus_tag="AC161X_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003996217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR4988886.1"
                     /translation="MSTHIVKKAGDHDYQVPGGFDGRSQGYLGDAIVNEAAGGVQMGF
                     QVSRLDPGGQVDGHLHSFEETLYVMDGMVVVDLPEGSFELRPGDYGLVPVGVPHSLRN
                     TGGGVATFAEMRAPLPRERHEFDTYFLDVPSAGSPLRIDARDPRTRSFGHIDAENMDP
                     ALQTQDRLALSASMRSALLVYSGISVKMMIDSDLGADLSTMFMVQYVPGGFAGAHDHP
                     LEEAYLILEGEVEAGFDGVKYHMVPGDVAWAGVGCVHEFRNVGTGPVRWLETQAPQPP
                     ARHSYRFRRDWAYLTEALAASD"
     gene            1508..2800
                     /locus_tag="AC161X_04735"
     CDS             1508..2800
                     /locus_tag="AC161X_04735"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR4988887.1"
                     /translation="MKGKTLLRGGCVLTLGKTNYAEADVLIDGETIVEIGPGLRSRDA
                     EVVDATDTIVMPGFVDSHRRVSESLFRNFGDSTSSEVYGPHYQADDAYAATLIGLLGA
                     IDSGITTVVDWFGLGSSEELVDAALQAHADSGIRSVFAYAGRGADGWQSDLKSITARA
                     TPQPLLDLAAGAGTPVRDSMDEVTADWSVARDLGLRIHAQVGVDSEVGPGMVREMSER
                     GLLAGDVNLVQCTYLDDADFDAIASSGVSVSVTPSSAMSGGQARPPMQEMIDRGIRPG
                     LGVGSEQFAPGDMFAQMRAVISVQHATLFDLKLAGKAGIPTLLNTREVLRYATINGAR
                     AAGVSDRVGTLAPGSQADIILLRTDTPNIFPINDPIGAVVWGMDTSNVDWVFVAGRPL
                     KRAGVLQGDLDLAVENANSAQQRVAEAAGLLVGSGEMA"
     gene            2797..3846
                     /locus_tag="AC161X_04740"
     CDS             2797..3846
                     /locus_tag="AC161X_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988888.1"
                     /translation="MTTTKSPSSALTRFVVASRYMPVYLALILLVVVASIAAPATLSE
                     AGLSAIAPLGTYLAIAALGQMLVIMTGGIDLSVPGTFLLGGLIMVGVGQQSDDRIGIA
                     FLTAVGVAAAIGLVNGLMIGGLKLNPLIVTLAVGQIVAGVANRYYNTVAIQKPVPEGL
                     TDWATTTFLGVSRIFWIGVALTLVLVVIFRYTGAGRRFQVVGANPVASTVIGLRVNLN
                     QIAAYMVAAVLYATAGMLLAASLRSPGITVGAPYLLGPIAAVVIGGASLTGGLASPVS
                     TWAAAFFLAGLNQMMRVIGLPTALQFVVFGLVIIGGMLVSGDRIIKSVERVLRDRSRP
                     GRRNEGTLAVEGGET"
     gene            3915..5258
                     /locus_tag="AC161X_04745"
     CDS             3915..5258
                     /locus_tag="AC161X_04745"
                     /inference="COORDINATES: protein motif:HMM:NF024799.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HIR4988889.1"
                     /translation="MKKYRVSTLIAVLALVMVACTGDVTESSTTAAAEPTTTAAPEPT
                     TTVGGETSTTAGGEDPLAAYGETTAADPALVEKAIGPVDEVPDIVLASIARANQDLDD
                     ETIAKAIECYTNAVECDTGTGGEVIMGYADGGGLNVWRQVTRMEAILQALTYPEIGTI
                     VMRDAQWSTDPAVPIGDINYMVERGVTFIIGYPDWGSALGDAIKAAEAAGIPYMSYSA
                     GYVGLPGTDGALAPGTDYLGVVGEDLCALGESFATIINEGVGTGTIGVMGGTPGNALS
                     LGWQQCHIPALADGIDVIDPPADPNSTTGDTYWANDIAKDLVSGWLSTNPDLAGFSYE
                     YADGLYTALQDVEIDTMTIAIRTDEQTLFCDWAERNNPNYNIWYSAGGNFQSRVAVTA
                     QMMNIQGAEIPGEIIVPHVMRQVTTDDCNPDRVTPTVSGTSLVPDSVLAQMYPAG"
     gene            5255..6850
                     /locus_tag="AC161X_04750"
     CDS             5255..6850
                     /locus_tag="AC161X_04750"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR4988890.1"
                     /translation="MSNGDLGGESATGKPVLELDGVYRQFGAVRALTDVSFDCLAGEV
                     HALVGENGSGKSTLLGIASGFVEPDRGSVRIGGARLRTDSPALARKLGLAMAYQDTSS
                     ILAQPVKNNLYMAAPPDERPPYWRRKKWARRLLGEFDLDFELFPDAPVGFLTLAERQM
                     FEVAKALVSNPKVLLLDEPTTALGPDEVEALHRTVAACRRRGVGVVYVSHRLPEILEI
                     ADRITVLRDGKTRGTFDARSTSERDLVELIVGRPFEAAFPPASPVSSERREVLVVDAL
                     QGQSFGPVSFTLESGEVVGIAGAEGNGQPQLFDTLSGRTPPRAGRVICDEKGLTLIST
                     HEAVNSGIMLLPGDRKQEALMPVLGVRVNSTVQSLKRFSVFGLLKRRAERAAVKDLVT
                     RLEVRTPSLEQPVEFLSGGNQQKVSVSRTFLREPAVILAYEPTQGVDVGSRFDIYKAL
                     RARTDAGAALLVKSSDPIELSGLCDRVLVMSRGTIIEEIPGDELDEMRIVEAIVRGPG
                     LSRAGRSPFGVAMPKAPSKEVVS"
     gene            6847..>8474
                     /locus_tag="AC161X_04755"
     CDS             6847..>8474
                     /locus_tag="AC161X_04755"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988891.1"
                     /translation="MTNYIDSARQEVRKVLRNARDKNKWRKVIKVRLWMPVALQILLI
                     FGVVWYTNSRFPGFVNSSNIANILLLAVPLAIVVIGQTHALLVGYLDLSVGAMISFGV
                     VIASFWIPADASTSQILTGVGTIFACGLVLGLVNAGLVRGVKIPSIIATLATLSILDG
                     ISLTLRPTPGGTIDPGFTSLLRGGIGPVPLAFILVVLGAGALDLWLHSSGSGLQTRSV
                     GFDERSARRSGVRTTWVRVRALVLSASFAALASFFVMTRSGVGNAQIGSSYALNSITA
                     AVLGGAALSGGRATFTGGVVASVLLAVIITVLPFLGLGPEYGLMIIGVLVLIGIILFQ
                     VGDIKELVKRNYRRARRIVVGTRPPVAPELPVLYPVGTDFKVMPVGRKLISGGIVLSL
                     DSSVGDFSNADILIDGDRILAVGPNLQNGEAEHIDATGMIVMPGFVDSHRHTWEGILR
                     NIGTDVPLEGRTSYISFVLHKLAPAFRPEDVYAGNLVSTLGAIDAGVTTLLDWSHIQA
                     SPAHTDAAVKALGDSGMRAVFAYGFPWWGKWEER"
BASE COUNT         1471 a         2573 c         2711 g         1719 t
ORIGIN      
        1 gtcccggcgt cgatgtcgta ctccttggcg aagttgttat ccctggcaat ggcggccagg
       61 accagacgcg atacggtccc gacctcgtgg agggcggggg cgatggctga aggatcggtg
      121 agatcgaacc cgatgccagt cacgtcgcct tcgatagttg aagcgatctc cccggtgcga
      181 ccagggtcac tccccgtgat caccacttgg ctcccgccag ctgcatagtg gcgagccacc
      241 tccagcccga ggccactggt tccccccacc acaatcacgt tgccgttttc catcattgaa
      301 tcctgtttct ccgcgtatcc gacgccggct ggccgggggg gcgaagccta caaccgtttg
      361 gccgagcgtg tgtagtgtca ctgatcggtc gagtgattgg gagccaaatg tcgacgcaca
      421 tcgtcaagaa agccggggat cacgactatc aggtgccggg tggctttgac gggcgaagcc
      481 agggctacct cggggatgca atcgtcaacg aggctgcagg tggggttcag atgggattcc
      541 aggtttcgcg gctcgatcct ggtgggcagg tcgacggcca ccttcactca tttgaggaga
      601 ccctctatgt gatggacggg atggtcgtcg tcgatcttcc cgaagggagt ttcgagctcc
      661 gcccgggcga ctacgggttg gtcccggtgg gtgttcccca ctcgcttcgg aacacaggcg
      721 ggggagttgc gacttttgcg gagatgaggg cccctctccc gcgcgagcgc cacgaattcg
      781 acacctactt cctggatgtg ccgtcggcgg ggtcccctct tcgcatcgac gcccgtgacc
      841 cgcgaacccg cagcttcggc catatcgatg ccgagaacat ggatccagcc ctgcagaccc
      901 aggatcgcct agcgctcagt gcgagcatgc gctccgccct tctcgtttac agcgggatct
      961 cggtgaaaat gatgatcgac tccgacctag gagcggatct gtcgacgatg ttcatggtcc
     1021 agtacgtgcc gggagggttc gccggagccc atgaccatcc gctcgaggag gcctatctca
     1081 tcctcgaagg ggaagtcgag gcggggtttg acggcgtcaa atatcacatg gttcctggtg
     1141 acgtggcctg ggccggagtg gggtgcgttc acgaattccg aaatgtcggc accggcccgg
     1201 tgcggtggct cgagactcag gctccacagc cgcccgcccg gcatagctac cggttccgga
     1261 gggactgggc gtacctgacc gaggccctcg ctgcctcgga ttgaaactgg ttgtcggcca
     1321 atgttgtgtg agctctcggg acctgttttg tgaggctgaa ccggatgcga tttgtgtagt
     1381 gtgacttatc agtgagtggc gttaacaccc cgctatcggc cgccgcctcc aacggagaat
     1441 tggcactgcg tttgcccgat tcacatcgtg tgccgttgat gaaccgaccc caggtcggag
     1501 cgaacccgtg aaagggaaga cactgctccg cggcgggtgt gtgttgacgc tcggtaagac
     1561 caactacgcc gaagccgatg tcttgatcga tggggagacg atcgtcgaga tcggccccgg
     1621 gctccggtcc cgtgatgcgg aagttgtcga tgccacggac accattgtca tgccgggatt
     1681 cgtggactcc catcgccgcg tttcggagtc actgttccga aacttcgggg actcaacctc
     1741 gtcggaggtc tacgggcctc actatcaggc cgatgacgcg tatgccgcca ctctgatcgg
     1801 gctgctcggt gcgatcgatt ccgggatcac cacggtggtc gattggttcg gccttggttc
     1861 ctcggaggag ctggtagacg ccgcgcttca ggcgcacgcc gactccggaa tccgatccgt
     1921 ctttgcctac gccggccgag gggccgacgg atggcagtcc gatctcaaga gcatcactgc
     1981 gcgggccacc ccccaaccgc tgctcgatct agctgctggt gctgggacgc cagtacgtga
     2041 ctcgatggac gaagtgaccg ctgattggtc tgtggcccgg gatctcggtc ttcgaatcca
     2101 tgctcaggtc ggcgtcgact ctgaggttgg cccgggcatg gttcgggaaa tgtcggagcg
     2161 aggcctgctc gcaggggacg tcaacctggt tcagtgcacc tacctggacg atgccgactt
     2221 cgacgcgatc gcctcgtccg gtgtttccgt ctcagtaacc ccgtccagcg cgatgtctgg
     2281 gggtcaggcg aggccgccga tgcaggagat gatcgatcgt ggcattcgcc ccgggctggg
     2341 cgtgggctca gagcagttcg cccctggtga catgttcgcc cagatgcggg ccgtgatctc
     2401 ggtgcagcac gccaccctgt tcgacctcaa gctggcggga aaggcaggga tcccaactct
     2461 tctcaataca cgcgaggtgc ttcgctacgc cacaatcaac ggtgctcggg cagccggcgt
     2521 cagcgacagg gtgggaactc ttgcccccgg gtcgcaggcc gacatcatcc tgctccgcac
     2581 cgatacgcca aacatctttc caattaacga tccgatcgga gcggttgtct ggggaatgga
     2641 cacctcgaac gtggattggg tgttcgtcgc aggtcgccca ctaaagcggg caggtgtcct
     2701 ccagggtgac ctcgacctgg ccgtcgagaa tgcgaattcg gcccaacaaa gggttgccga
     2761 ggctgccggg cttctcgtcg ggtccggaga gatggcatga cgaccacgaa gtcgccgtcc
     2821 tcggccctca cccgttttgt cgtggcctcc cgctacatgc cggtctatct ggctttgatc
     2881 ctgcttgtgg tcgtggcctc gatcgcggct cctgccacat tgtccgaggc agggctgagc
     2941 gcgatcgctc ctcttggaac ctatctggcc attgcggccc tcggccagat gctggtgatc
     3001 atgaccgggg ggatcgatct cagtgttccg ggaaccttcc tcttaggtgg gctcatcatg
     3061 gtgggagttg gtcaacagtc cgatgatcgg atcgggattg cctttctcac cgctgttggg
     3121 gtggccgcag ccatcggtct ggtgaatggg ctcatgatcg gtggtctcaa gttgaacccg
     3181 ctcatcgtca ctctggccgt cgggcaaatc gtggccgggg tggccaaccg ttactacaac
     3241 accgttgcga ttcagaagcc ggtgccggaa gggctaactg attgggctac gaccaccttt
     3301 ctcggtgtca gccggatctt ctggatcggg gtggcgttga cgctcgtcct ggtcgtgatc
     3361 ttccgataca ccggtgcagg tcggagattt caggtcgtgg gggccaaccc ggtcgcgtcc
     3421 acggtcattg ggttgcgggt caacctcaat cagatcgccg cctacatggt ggcagcggtc
     3481 ctttacgcga cggccgggat gttgctcgcg gcttccctgc ggtctcccgg aatcactgtt
     3541 ggagctccgt atctgctggg cccgatcgcg gcagtggtga tcggcggagc atcgctgacc
     3601 ggtggtctgg cgagtccggt gtccacctgg gcggccgcat tcttcctcgc cgggctcaac
     3661 cagatgatgc gggtgatcgg gctgccaacc gccctccagt tcgtggtctt cggcttggtg
     3721 atcatcggtg ggatgttggt gtccggtgat cgcatcatca agagcgtcga aagggttttg
     3781 cgcgaccggt cgaggccggg gaggcgcaat gagggaactc tcgccgtcga aggcggcgag
     3841 acgtgagaga aggagcaact gaccgcaaca caagagcggg agaaatagcc acagtcaatg
     3901 aatggaggag gatgatgaag aaatacaggg tgtcaaccct gatcgccgta ctggcactcg
     3961 tcatggtcgc ctgtacgggc gatgtgaccg agtccagtac cacggcggca gcagagccga
     4021 cgacgaccgc ggctccggag ccgaccacca cggttggcgg tgagacttcg acaaccgccg
     4081 gcggcgagga tccgctagct gcctacggag agaccaccgc agccgacccg gcattggtgg
     4141 agaaggcgat tggcccggtc gacgaagttc ctgacatcgt cctcgcatcg attgcgcgtg
     4201 cgaaccagga tctcgacgac gaaaccattg ccaaggccat cgagtgctac acgaatgcag
     4261 tcgagtgcga cacaggcaca ggcggtgaag tgatcatggg atacgccgac ggtggaggtc
     4321 tcaacgtctg gcgtcaggtg acccgcatgg aggcaatcct tcaggcgctc acctatcccg
     4381 agatcggcac catcgtcatg cgtgacgcac aatggtcgac cgaccctgcg gttccgatcg
     4441 gtgacatcaa ctacatggtg gagcgtgggg tcacgttcat catcggatac cccgactggg
     4501 gatctgccct tggtgatgcg atcaaggccg cagaggcagc gggcatccca tatatgtcct
     4561 attcggccgg ctacgtcggc cttcccggga ctgatggtgc gctcgcaccc ggtactgatt
     4621 atctgggagt tgtcggcgag gacctctgtg ccctcggcga gagctttgcc acgatcatca
     4681 acgaaggggt tggcacgggc acgatcggcg tcatgggtgg gacaccgggc aacgccctgt
     4741 ccctcggatg gcagcagtgc catatcccgg cgctggcaga tggcatcgat gtcatcgacc
     4801 cgcccgcgga tccgaactcc acgaccgggg acacctactg ggccaacgac atcgcaaagg
     4861 atctcgtcag cggatggctt tcgaccaatc cggatctcgc cgggttcagc tatgagtacg
     4921 ccgacgggct gtacaccgcg cttcaggacg tcgagatcga cacgatgacg atcgcgattc
     4981 ggaccgatga gcagaccctg ttctgcgact gggccgagcg aaacaacccg aactacaaca
     5041 tctggtactc ggcgggaggc aacttccagt cgagagtcgc tgtgacggct cagatgatga
     5101 atatccaggg tgcggagatc ccaggggaga tcattgttcc tcacgtgatg aggcaggtca
     5161 ccactgacga ctgcaacccg gatcgggtaa caccgaccgt ttctggcacc tcactggtac
     5221 ccgactcggt ccttgcccag atgtatccgg ctggatgagt aacggcgatc tcggaggaga
     5281 gtcggcgacc gggaaaccgg tcctcgagct tgacggtgtc taccgccagt tcggcgcggt
     5341 tcgagcgctg acagatgtca gcttcgactg cctggctggt gaagttcacg ccctcgtagg
     5401 cgagaacggc tcgggcaagt cgaccctcct cgggattgcc agcgggttcg tagagcccga
     5461 ccgtggatcg gtgcgcatcg ggggagcccg gttgcgcacc gattctccgg ctctcgccag
     5521 aaaactggga cttgcgatgg cctatcagga cacctcgtcg atcctggcgc agccggtcaa
     5581 gaacaacctc tacatggccg ctccgcccga tgagcggccg ccttactgga gacggaagaa
     5641 gtgggcgcgg cgtctgctgg gcgagttcga cctcgatttc gagctgttcc cagatgcgcc
     5701 tgttggattc ctgactctcg ccgagcgtca gatgttcgag gtcgccaagg cattggtatc
     5761 gaaccccaag gtgctgcttc tcgacgagcc cacgacggcg cttggccccg atgaggtcga
     5821 ggcattgcac cgtaccgtcg ccgcttgccg gcgccgcggt gttggcgttg tctatgtcag
     5881 ccatcgtctg cctgagatct tggaaatcgc ggaccggata accgtgcttc gcgacggaaa
     5941 gacgcggggg acctttgacg ctcgatcgac ttccgagcgt gatctggtcg agctcatcgt
     6001 cggtcgcccc ttcgaggcag cgttcccccc tgcatcgcct gtcagcagcg agcggcgaga
     6061 ggtgcttgtc gttgatgccc tccagggcca gtccttcggc ccggtcagct ttacccttga
     6121 gagtggagaa gtggtcggca tcgccggtgc cgaaggcaac ggtcaacccc agctcttcga
     6181 caccctttcc gggagaacgc caccaagggc cggccgggtg atttgtgacg aaaagggcct
     6241 gaccctgatt tcgacacacg aggcggtcaa ttccgggatc atgttgctgc ccggagatcg
     6301 caagcaagag gcgttgatgc cggtgctcgg ggtgcgggtc aactccacag tccagtcact
     6361 gaagcgtttc agcgttttcg ggctcctgaa gagacgcgcc gaaagggctg ctgtgaagga
     6421 tctggtcact cggctcgagg tccgcacccc ttcgttggag cagcccgttg agtttctttc
     6481 cggcggcaac cagcagaagg tctcagtctc tcgaactttt ctcagggagc ccgcggtcat
     6541 cctcgcctat gagcccacgc agggggtgga cgtcgggtcc cgtttcgaca tttacaaagc
     6601 cctccgggcc cggaccgacg ccggtgccgc gctactggtc aagtcgagcg acccgatcga
     6661 gctctcggga ctctgtgatc gggtgctggt gatgtcacgc ggcaccatca tcgaagagat
     6721 tcccggtgac gagctcgacg agatgcgcat cgtcgaggcc atcgtccgcg ggccgggact
     6781 gtcacgcgcc gggcggtctc cattcggtgt cgcaatgccc aaggctccgt cgaaggaggt
     6841 cgtgtcatga cgaactacat cgattcggcg cgacaggagg tgcgcaaggt cctgcgcaat
     6901 gcccgcgaca agaacaaatg gcggaaggtc atcaaggttc gcctctggat gccggtcgcc
     6961 ctccagatcc tcctgatctt cggagttgtt tggtacacca actcccgctt ccccgggttc
     7021 gtcaattcat cgaacatcgc aaacatcctc ctgctcgccg tgccgctggc gatcgtcgtc
     7081 atcggccaaa ctcacgccct gctcgtcgga tatctcgatc tttctgtagg cgcgatgatc
     7141 agttttggcg tggtgatcgc ctcgttctgg atccccgccg acgcgtctac cagccagata
     7201 ctgaccgggg tggggacgat ctttgcctgc gggctggtcc tcggcctggt caatgccggg
     7261 ctggtccgtg gggtcaagat cccatcgatc atcgccacgc tggccaccct cagcattctc
     7321 gatgggatct ctctaactct gcgtcccact cctgggggaa ccatcgatcc cggattcaca
     7381 tcgctgttac gcggcgggat cggtccggtg cccctcgcct tcatcctggt ggtcttgggt
     7441 gctggtgccc tcgacctctg gcttcactcg agcgggtccg ggcttcagac gcgctctgtg
     7501 ggattcgacg agcgatcggc gaggaggtcc ggcgtccgga ctacctgggt gagggtccgc
     7561 gccctggttc tctccgcatc gttcgccgcc cttgcctctt tcttcgtgat gactcgctcc
     7621 ggggtgggca atgcgcagat cggatcaagc tatgccctca acagcatcac ggcagcggtg
     7681 ctgggtgggg ccgcactctc cgggggaagg gcaactttca ccggaggggt ggtcgcgtcg
     7741 gtcctgctcg ccgtgatcat caccgtgctc cccttcctcg gcctcggccc ggagtacggg
     7801 ctgatgatca tcggcgtcct ggtcctgatt gggatcatcc tcttccaggt tggtgacatc
     7861 aaggagttgg tgaagcggaa ctaccgcagg gcccggcgga tcgtcgttgg cacccgtcct
     7921 cctgtcgctc ccgagcttcc cgtcctctat cccgttggaa ccgacttcaa agtgatgcca
     7981 gtcggtcgca agctgatcag cggtgggatc gtgctcagcc tcgactcgag cgtcggggat
     8041 ttcagcaacg cggacatcct catcgatggg gacaggatcc tcgcggttgg gcccaacctc
     8101 cagaatgggg aggccgagca catcgacgcc acggggatga tcgtcatgcc cgggtttgtc
     8161 gactctcatc ggcacacctg ggaaggcatc ctgcgcaaca tcggcaccga cgtgcctctc
     8221 gaggggcgga ccagttacat ctcgttcgtc ctccacaagc tggcaccggc ctttcgaccc
     8281 gaggatgttt atgcggggaa ccttgtttcg acactgggag ccatcgacgc cggggtgacc
     8341 acacttctcg actggtctca cattcaggcc tcgcctgccc acactgatgc cgcggtcaag
     8401 gcattgggag actccgggat gcgcgccgtg tttgcctacg ggtttccctg gtgggggaag
     8461 tgggaagagc gcca
//
LOCUS       DBNHXU010000189         5962 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2837330, whole genome shotgun sequence.
ACCESSION   DBNHXU010000189 DBNHXU010000000
VERSION     DBNHXU010000189.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5962)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5962)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5962
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2837330"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..779
                     /locus_tag="AC161X_04760"
     CDS             <1..779
                     /locus_tag="AC161X_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015064848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate dehydrogenase"
                     /protein_id="HIR4988892.1"
                     /translation="LGAIGRVVVSRLVQGVPGVELTAVASRDIARAEATIRDLGADIP
                     VRPLDQLEPLADLVVECAPAALLPVIVEPFLRSGKEAVVLSSGAILEHPELVDLAKEH
                     GGQITIPSGALLGLDAVAAAAEGEIRSLRLTTRKPPAGLVGAPGLEESDVDLQGLTEP
                     VLVFAGTARDAIRGFPANLNVAVALSLAGPGPDRTDVEVWADPTVTKNTHIIVMRSDV
                     ADVTMTIENVPSENPRTGRITPLSIISLLRKRHASLRVGS"
     gene            776..1561
                     /locus_tag="AC161X_04765"
     CDS             776..1561
                     /locus_tag="AC161X_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017957495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4988893.1"
                     /translation="MNLGLSGKTALVVGGSNGLGRGVALTLAREGARVAVAGRDLDAA
                     ATTVAMIAAEGGEAMPVRLDLTDFSSLDAAVETIRSHFGDVAILFNNSGGPPPSTAAN
                     QPVDLWRAQFEAMVLGVIALTDRILPGMRKAGWGRIVTNTSSGVLAPIPNLAISNALR
                     LSLVGWSKTLAREVAPEGITVNIVVPGRIATGRVRALDEARAARENTSVEAAEAASIS
                     AIPIGRYGDPEEFAAAVAFLASAQASFITGTMLRVDGGQTPSI"
     gene            1573..2256
                     /locus_tag="AC161X_04770"
     CDS             1573..2256
                     /locus_tag="AC161X_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HIR4988894.1"
                     /translation="MNLSGEQAEEIRRRFLSVDTSNVADVLDELGLPDQGLAPSFSPF
                     PSGAGKLAGWAYTIRGEMAPYPLGAGDPAKMEACAQLTPGSVSVWSGGGEGVCFFGEL
                     ISIGMKERGCVGALVDGGIRDIDWIDALGFPVYARYRTPVQSIGRWRVTDSGETVPMP
                     GATVPEVQVTPGDFILADSDGAIVIPSGVVEEVLERAEALGAREVQIRAELANGLSLS
                     EALARFGHV"
     gene            complement(2263..2649)
                     /locus_tag="AC161X_04775"
     CDS             complement(2263..2649)
                     /locus_tag="AC161X_04775"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03618 family F420-dependent PPOX class
                     oxidoreductase"
                     /protein_id="HIR4988895.1"
                     /translation="MLSERTQRLAGGPNHAVLTTLFPSGQPQSHIVWVDHDGENLLVN
                     SETLRQKVQNVQNDPRVSVVLFNAEDFDDWVEIRGRVVEIVGGDEARDHIDKLSEKYI
                     GLSDYPNPIRSPRVILKIAPTSEKVY"
     gene            2793..4091
                     /locus_tag="AC161X_04780"
     CDS             2793..4091
                     /locus_tag="AC161X_04780"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018838021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR4988896.1"
                     /translation="MTNAGVIRGDLKVDSERIAAIGDVDPEGEIVDVSGLIILPGMVD
                     THVHLMDPGDTGREDFIHGTRAAAARGVTTIVEHTHAHPIRGVPDLEEKTAHLRTRSN
                     VDFGLAAHVWPDRIPEIGSLVAAGITFFKIFTCSTHGVPGIEHDQLSEALTAIKQAGG
                     RCLVHNEDQQLTTDAERRLKESGRTDPGLLLEWRTRDAELEATAATAAAVLDSGVAAT
                     FAHVSSTGVLDVIDAFRLRGADIAAEACPQYLALEEDEVIDKGALRKFTPPARIRDES
                     DRSEMWDAVVDGRFSHFSTDHAPSTVEQKEAGDFWSAPFGLPGLDTTLPFLLDAALSG
                     RIGLHDLARLYAAAPAARYGLSKGVLAPGSDADLVLVDPTGSWTVRDEDILSKAGWSP
                     FSGKTLQGRVVATFLRGVEVARDGRCHDLRTGRFIRPSRS"
     gene            complement(4095..5267)
                     /locus_tag="AC161X_04785"
     CDS             complement(4095..5267)
                     /locus_tag="AC161X_04785"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR4988897.1"
                     /translation="MSITNTEFARRHEGAKSALVEGEFDALIAYSTAKVAANVRYLTD
                     YFVRFTGMQNMREGGYHMFGSSAVLFPTDGEPMVRTDQPWDIARAKEISMFPDTEYAG
                     LFAQDFGRVIRERGYRRVAIDNWYIFPAREFMALQEMAPDVEFVPSHLMSELRRIKSE
                     EELAIIRRSAALAVEAVETSLDTVQVGANEYDVVLLCEETMRRGGDIELAGGSISGCG
                     PNTSTGSWVPSNTQSRAMAPGEWVMMDVCPRVDGYAGDISRHRVAGDVSDLDPRLKKM
                     YDVALVMSEEVRKAIKPGVSGRELNSLAEQIADGEGFLENKIGLLGHGLGLDIHDIPD
                     FYYDESLLSPGEVITVEPCLLLEGVGGVRIEDMVLVTGDGSETLTSAADRELRGSS"
     gene            5403..>5962
                     /locus_tag="AC161X_04790"
     CDS             5403..>5962
                     /locus_tag="AC161X_04790"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4988898.1"
                     /translation="MATKRVWLALLLAIGLAACTADDSGGSSTTAGSAASSTTVGSTT
                     ATTTSGGEELPTLKIGIPLPFTGVVAQSAEEMRQTFEMYVEQQGGTLGGLPVEVIFED
                     TETDAELVVTKTRKLIDEDQVHLLSGPMLAFEGLAMIDSVNAAGIALVGQTGVAADDY
                     TRLDAPTLTAAAKHTASQETMPLGTYA"
BASE COUNT         1106 a         1900 c         1858 g         1098 t
ORIGIN      
        1 gcctcggagc aataggccgt gttgttgtga gccggttggt gcagggggtg cctggggtgg
       61 agctgaccgc cgtggcctca cgcgacatcg cacgggccga ggcaacaatt cgagaccttg
      121 gcgccgatat acccgttcgc ccgctcgatc agttggagcc tttggccgac ctcgtcgtcg
      181 agtgcgcacc ggccgccctc ctgcctgtga tagtcgagcc cttcctgcgg tccggaaagg
      241 aggcggtcgt cctcagctct ggagcgattc tcgaacaccc ggaactggtc gacctggcca
      301 aagagcatgg aggccagatc accattccgt ctggagctct cctcggactc gatgcagtgg
      361 ctgccgctgc cgagggtgag atccgatctc tgcgactgac aacgcgcaag ccgccggcgg
      421 gcctggtcgg cgcccccggg ctcgaagaaa gcgacgtcga cctccaaggc ctgaccgagc
      481 ctgtgcttgt gttcgccggc accgcccggg atgcaatccg cggattcccg gcgaatctca
      541 acgtcgcggt tgcgctctcc cttgccgggc ctggccccga ccggaccgac gtagaagtgt
      601 gggccgaccc gacggtcaca aagaacacgc acatcatcgt gatgcgctct gatgtcgccg
      661 acgtgacgat gacaatcgaa aacgtgccct ctgagaatcc gcgaactggc cggatcaccc
      721 ccctgtcgat catcagcctg ctccgcaagc ggcacgcaag cctgagggtc ggttcatgaa
      781 ccttgggctg tcaggaaaga ccgctttggt cgtgggaggg agcaacggcc tcggacgggg
      841 cgtagctctg accctggctc gcgagggagc ccgggtggca gtggccggtc gcgatctcga
      901 tgccgccgcc acaactgtcg ccatgattgc ggccgaaggt ggcgaggcaa tgccggtccg
      961 cctcgacctc accgacttct cctctctcga tgctgcggtc gaaaccatcc gcagccactt
     1021 tggcgatgtg gcgatcctgt tcaacaacag cggaggccca cccccttcca ccgccgccaa
     1081 tcagccggtc gatctgtggc gggcccagtt cgaggcgatg gtcctcggag tgatcgcact
     1141 caccgatcgc atcctgcccg ggatgcggaa ggcgggttgg ggtcggatcg tgaccaatac
     1201 cagctccggc gtgttggcgc cgatcccgaa cctcgccatc tcgaacgccc tcaggctctc
     1261 gctcgttggt tggtccaaga cgctggcccg agaagtcgcg ccagagggga tcaccgtcaa
     1321 catcgtggtg ccgggtcgga tcgccaccgg tcgagtgcgc gcactagatg aggcacgggc
     1381 cgcacgggag aacacgtcgg tcgaagccgc tgaagcggcc agcatctctg ccatcccgat
     1441 cggccgctac ggggatcctg aggagttcgc cgccgcggtg gccttcctcg ccagcgctca
     1501 agcctctttc attaccggaa ccatgctgcg agtggacggc ggccagaccc cctcgatctg
     1561 aaggagcagg aaatgaacct gagcggcgaa caggctgaag agatccggag gcgattcctc
     1621 tcggtcgaca cgtcaaatgt tgccgacgtc ctcgatgagc tcggcttgcc cgaccagggc
     1681 ctggcgccga gtttctcccc gtttccatca ggtgcgggaa agctcgccgg atgggcctac
     1741 acgatccgtg gggaaatggc cccgtatccc cttggtgccg gcgatccagc caagatggag
     1801 gcctgcgccc agctgacccc tgggagcgtc tctgtgtgga gcggcggtgg agaaggggtc
     1861 tgctttttcg gcgagctcat ctcgataggg atgaaagagc gcggctgcgt cggcgccttg
     1921 gtggacggtg gaatccggga catcgactgg atcgacgccc tcggattccc ggtttacgcc
     1981 cgctaccgaa ccccggtcca gtcaattgga cgatggaggg tgaccgatag cggcgagacg
     2041 gttcccatgc ccggggcaac ggtcccagaa gttcaggtga ctcccggcga cttcatcctg
     2101 gccgacagcg acggagcgat cgtgatcccg tcaggggtgg tggaggaggt cttggagcgc
     2161 gccgaggcgc tgggggctcg tgaggttcag atcagggccg agctggcgaa cgggctgtcg
     2221 ctgtccgagg cattggccag gttcggccac gtctgaggcg aactagtaga ccttctcgct
     2281 ggtgggagcg atcttgagaa tcaccctcgg cgagcggatc ggattcgggt agtcgctgag
     2341 cccgatgtac ttctcggaga gcttgtcgat gtgatcccgg gcttcatcgc ctcccacgat
     2401 ctccacgaca cgtcccctta tctcgaccca gtcgtcgaag tcctccgcgt tgaagagcac
     2461 gaccgagacc cgagggtcgt tttgaacgtt ttgcaccttc tggcgaaggg tctcggagtt
     2521 gaccagcagg ttctcgccat cgtggtcaac ccacacgatg tggctttgag gctgaccact
     2581 cgggaagagc gtggtcagca cggcatggtt ggggccaccg gcaagtcgtt gggttcgctc
     2641 agacagcatg cggcaacgtt acccatctcc ccatcaggcc caacacaggc ttggcaggca
     2701 ccatcggctg aatccgacaa ttgcaccgat atgttgtctg cctaggaccg aggcctgata
     2761 tcgcaacgac gacgttccgt ggagcgacgc tggtgaccaa cgccggcgtg atccgtggcg
     2821 acctaaaggt cgattccgaa cgcatagccg ctatcggtga tgtggatccg gagggcgaga
     2881 ttgtggatgt cagcggtctg atcatcctcc cagggatggt tgacacgcac gttcatctga
     2941 tggatcccgg tgacaccggg cgcgaggact tcatacacgg gaccagagca gctgcggcac
     3001 gcggggtaac gacgatcgtc gagcacactc atgcccatcc cattcggggc gtacctgacc
     3061 tcgaggagaa gacggcgcac ctccgcactc gatccaatgt cgacttcggt ctcgccgccc
     3121 atgtctggcc agaccgcatc ccagaaatcg gctccctggt cgccgccggt atcacgttct
     3181 tcaagatctt cacttgctcc acgcacggtg ttccaggaat cgaacacgat cagctctcgg
     3241 aggcactcac agccatcaag caagcgggcg gtcgctgcct agttcacaac gaggaccagc
     3301 aactcacgac tgatgcggag cgcagactca aggagtcggg ccggaccgac ccgggcttgc
     3361 tcctggagtg gcgcacccgt gacgctgagc tcgaggcaac cgccgctacc gcagctgccg
     3421 tcctcgacag tggggtcgcg gcaaccttcg ctcacgtctc ctcaaccggg gttctcgacg
     3481 tcatcgacgc cttccggctg cgtggggcag acatcgcagc tgaggcctgc cctcaatatc
     3541 tcgccctcga ggaggatgag gtaatcgaca agggggcgct gcgtaagttc acaccgccag
     3601 cccggataag agacgaaagc gaccgttccg aaatgtggga cgcagtagtg gatggccgct
     3661 tctcgcattt ctcaacggac catgcgccat ccacagtgga gcagaaagaa gctggcgact
     3721 tctggagtgc cccgttcggc cttcccgggc tggacaccac actgcctttc ctgctcgatg
     3781 ccgccctcag cggccggatc ggattgcacg atttggcgag gctgtatgcg gctgcgcctg
     3841 ccgcgaggta cgggctctcc aaaggggttc tcgcccccgg ctccgacgct gatctggtcc
     3901 tcgtcgaccc aacagggtcg tggacagtgc gcgacgagga catcctctcg aaggcgggct
     3961 ggtcaccatt cagcggcaag acgctgcagg gccgggtggt cgcaaccttt ctacgtggag
     4021 tcgaggtggc acgagacggc cgatgtcacg atcttcgaac cggacggttc attcgtccga
     4081 gtcgaagctg acagctaact cgacccgcga agctcccggt cagccgcact cgtcagagtc
     4141 tcggacccgt cccccgtcac gagcaccatg tcctcgattc ggaccccgcc gacgccctcg
     4201 agcaggaggc acggctcgac ggtgatcacc tctcccggtg agagaagact ctcgtcgtaa
     4261 tagaagtcag ggatgtcgtg gatgtccaag ccgaggccgt gaccaagcag gccgatcttg
     4321 ttctctagga atccctcccc gtctgcgatc tgttccgcta gagagttcag ctcgcggcct
     4381 gacaccccgg gcttgatcgc tttgcgaacc tcttcactca tgaccagggc gacgtcgtac
     4441 atcttcttca accgcgggtc caggtcgctc acgtcacccg ccacgcgatg gcgggagata
     4501 tcgccggcgt acccgtcgac gcgtgggcag acatccatca tgacccactc accgggtgcc
     4561 atcgctcgac tctgtgtgtt cgacggaacc cagctcccgg tggacgtgtt cggtccgcat
     4621 ccggagatcg atcctcctgc gagctcgatg tcaccgccac gtcgcatcgt ctcttcgcag
     4681 aggaggacga cgtcgtactc gttggcacca acctgcaccg tgtcgagcga tgtctcgacc
     4741 gcctcgaccg caagggccgc tgacctgcga atgattgcga gttcctcttc tgacttgatg
     4801 cgacgcaact ccgacatcag atgtgacggg acgaactcca catcgggggc catctcttgc
     4861 agcgccatga actcgcgtgc cgggaagatg taccaattgt cgatcgccac tcgccgatac
     4921 ccgcgttcac ggattacacg gccgaagtct tgggcgaaga gacccgcgta ctcggtatca
     4981 gggaacatcg agatctcttt cgcccttgcg atgtcccagg gctgatcggt gcggaccatc
     5041 ggctcgccgt cggtcgggaa gaggacagcg ctcgagccga acatgtggta cccgccttcg
     5101 cgcatgttct gcatcccggt gaaacggacg aagtagtcgg tcaggtaccg gacgttcgct
     5161 gccaccttgg cggtcgaata ggcaatcagc gcgtcgaatt caccctcgac gagcgccgac
     5221 ttcgcccctt cgtgccgcct ggcaaactcg gtgttcgtga tggacatgag ctgactcctt
     5281 tggcccgcga gtgttccgtt tctcgtcccg cacatcgacc tcgctcagcg acccgcggta
     5341 cggcccaatg gtatagtctg cagaacgtag gattcagaaa cccaatgttc aaaggagact
     5401 tcatggcgac aaagcgcgtt tggctggcgc tattactcgc gatcggtctg gcagcatgca
     5461 ctgccgatga ctctggaggg agctcaacaa cggcaggctc ggcagcgagc tcgacaaccg
     5521 ttggctccac gaccgcgacc acgacatcgg gtggcgagga actgccgacg ctgaagattg
     5581 gaatccccct gccgttcacc ggcgtcgtgg cgcaatcggc cgaagagatg cgccagacgt
     5641 tcgaaatgta cgtcgagcag caaggtggca cactcggagg acttccggtt gaggtgatct
     5701 tcgaagacac cgagacagat gccgagctgg tcgtcaccaa gacaaggaaa ctcatcgacg
     5761 aggaccaggt ccacctgctt tcgggcccga tgttggcctt cgagggttta gcgatgatcg
     5821 actccgtcaa cgccgctggg atcgctttgg ttggacaaac cggtgtggca gcagatgact
     5881 acacccgtct cgacgccccg acgttgacgg cggccgccaa gcacaccgcc agccaggaga
     5941 cgatgccgct cgggacctac gc
//
LOCUS       DBNHXU010000190         3867 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4821921, whole genome shotgun sequence.
ACCESSION   DBNHXU010000190 DBNHXU010000000
VERSION     DBNHXU010000190.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3867)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3867
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4821921"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..602
                     /locus_tag="AC161X_04795"
     CDS             <1..602
                     /locus_tag="AC161X_04795"
                     /inference="COORDINATES: protein motif:HMM:NF016300.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HIR4988899.1"
                     /translation="GEVTGTPDTVEVTLGVSLLAENVQEATTLAAQKADALIAALTDG
                     GVDPKDISTADYSIYPEYDYSNNTQRLVGYRVSNTVRVKIRNIEETGSLIDAATAAGG
                     NDVVVSGLQFSIDDDAEMVKAARAAAWEDAMAKATQLAELSGQELGAAVSINETVSST
                     PPPVYYERAAADAAGGETPIEPGTSAVTISLAVNFTLGA"
     gene            complement(599..1762)
                     /gene="moeB"
                     /locus_tag="AC161X_04800"
     CDS             complement(599..1762)
                     /gene="moeB"
                     /locus_tag="AC161X_04800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002628624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HIR4988900.1"
                     /translation="MTYQELVAAAKSQIVEVEPSELESRLNQVTLIDVREADEYDQGS
                     IAGAIHVPRGVLESSIRGHIPDLEQEVVLFCAVGGRSALAALSLQQLGYTRVTSLAGG
                     FGQWKSEGYPWGTPKSLTTDQRGRYSRHTVLPEVGEAGQMRLLDSKVMVIGAGGLGSP
                     AALYLAAAGIGTLGIVDFDVVDLSNLQRQILHNVDRLGQSKVESARETLSALNPDVKI
                     VTYNERLSADNVLRILSGYDVIVDGGDNFPTRYLVNDASLHLKVPVVHGSIFRFEGQL
                     TVYDPYNGPCYRCQFAEPPPPELAPSCAEAGVLGVLPGIIGSLQAMEAIKLVLEMGDS
                     LVGRLLTYDGLSQEFLSLNIRRNPDCPACADEAHPPRLVDYDQFCVPAGVVSR"
     gene            1889..2704
                     /locus_tag="AC161X_04805"
     CDS             1889..2704
                     /locus_tag="AC161X_04805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HIR4988901.1"
                     /translation="MTTKTTGPDRLTDEQLNELLNLVKKVDSVELKLTIPDSTRRSAA
                     AALDVDPLNAQIRQVFFFDTIDLTLNKAGVVLRARRIQGRDGDSTVKLRPVAPDDLSP
                     ELRKNPAFGVEVDALPGGYVCSGTMKGVATAVAVRETAAGLRPIRKLFTKEQRAFYAA
                     HAPEGIELDDLLILGPITVFKLKLAPRDFNRGLVIEQWNYPDGSRIVELSTKCHPSEA
                     FQVAAEARAYLSGIGIDLSGEQQTKTAAALEFFINEARAENGGNTKTRKSPTS"
     gene            2747..3751
                     /locus_tag="AC161X_04810"
     CDS             2747..3751
                     /locus_tag="AC161X_04810"
                     /EC_number="2.1.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009599436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_function: GO:0043857 - N-acetylornithine
                     carbamoyltransferase activity [Evidence IEA];
                     GO_process: GO:0006526 - L-arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylornithine carbamoyltransferase"
                     /protein_id="HIR4988902.1"
                     /translation="MRHFLTTADWSRPELQALIDDARSLKQSPFGDSLAGKTIALLFL
                     NNSLRTRTSFDVGINQLGGHAVVLSPSGGMWPLEFADGAIMDGDAEEHVKEAAKVLSR
                     YCDLIGIRAFPKFQDWEEDRKDLILNSFATYSDVPVINMETIVHPCQELAHIMALEEQ
                     LGALDGKKYLLTWSYHPKPLNTAVANSALTIAAKFGMEVTLLCPTPDYVLDERYMEQA
                     RADATAQGHQVTISHDIEEAYADADVVYAKSWGALPFFGNWESEKPIRDSYRHFIVDE
                     QKMALTNDALISHCLPMRRNLIMTDAVFDGPNCFAYDEAEDRLHVQKAIMTALLGDKN
                     "
BASE COUNT          803 a         1246 c         1155 g          663 t
ORIGIN      
        1 ccggagaagt gaccggcacc ccggacacgg tcgaggtgac cctcggggta tcgcttctgg
       61 ccgagaacgt ccaggaagcg accaccctgg ctgcgcagaa ggccgacgcc ttgatcgcag
      121 ccctcactga cggtggtgtc gatcccaagg acatctcaac ggccgactac tccatctacc
      181 ccgaatacga ctactcgaac aacacccagc gcctggtcgg ctatcgggtg agcaacaccg
      241 tccgggtcaa gatccgcaat atcgaggaaa ccggctcact gatcgacgcc gcaaccgcag
      301 caggcgggaa cgacgtggtg gtgagcgggc tccaattcag catcgatgac gacgccgaga
      361 tggtgaaggc agcccgcgcc gctgcgtggg aggacgccat ggccaaagcg actcagctcg
      421 cagaactgtc aggccaggaa ctcggtgcag ccgtctcgat caacgagacc gtgagcagca
      481 cgccgccgcc ggtctactac gagcgggccg ccgccgacgc tgcgggtggc gagaccccga
      541 tcgagcccgg cacctcggca gtcaccatct ctcttgcggt gaacttcacc ctcggcgcct
      601 agcgggatac gactcccgca ggcacacaga attggtcgta gtcgacgagg cgcggtggat
      661 gcgcctcgtc ggcgcaagcg gggcaatccg ggttgcgtct gatgttcaga ctcaggaact
      721 cctggctcag cccgtcatag gtgaggagcc gcccaacgag gctgtccccc atctcgagga
      781 ccaacttgat tgcctccatg gcctgcaacg agccgatgat cccgggaagc actcccagga
      841 cgccagcttc ggcacaggat ggggccaact cgggtggtgg gggctcggcg aactgacacc
      901 ggtaacaagg gccgttgtag gggtcataca ccgtgagctg gccttcgaag cggaagatgg
      961 agccgtggac cacgggcacc ttgagatgga gcgacgcgtc gttaaccaga tatcgggtcg
     1021 ggaagttgtc tcccccgtcg acgatcacgt cgtagccgga caggatccgg agcacgttgt
     1081 cggccgatag gcgctcgttg taggtgacga tcttcacgtc ggggttgagg gcactcagcg
     1141 tctcccgggc cgactccacc ttcgactggc caagcctgtc gacgttgtgc aggatctgtc
     1201 gctggaggtt ggacaggtcg acgacgtcga agtcgacgat cccgagcgtg ccaatgcccg
     1261 ccgccgccag gtagagagcc gcgggtgatc cgagcccacc ggccccgata accatcactt
     1321 tcgagtcgag gagcctcatc tgacccgcct cccccacttc ggggaggacg gtgtgccggc
     1381 tgtagcggcc ccgctggtcg gtcgtcaggc tcttgggcgt cccccacgga taaccctcgg
     1441 acttccattg cccgaacccg ccagccagcg aggtaacccg ggtatatccc agttgttgca
     1501 gggagagggc cgccagggca gatcgcccgc ctaccgcgca gaagagcact acctcctgct
     1561 cgagatcggg gatgtgccct cgaattgagc tctccagaac accccgtggc acatggatgg
     1621 cgcccgcgat cgacccctgg tcgtactcgt ccgcctcccg aacatcgatc agagtgacct
     1681 gattgagacg tgactccaat tccgaaggct cgacctcgac gatttgactc ttggccgctg
     1741 ccaccagctc ctgataggtc atgctcaccg cggtcaacct ccgtcacgag agggtcgatg
     1801 acgagtatgg ccctgggcca aaagggggtc aagagcctgc ctccgagccc gctgcgtatc
     1861 atgacaacac cacaagcgga ggaatcaaat gaccaccaag acaactggcc cggatcgtct
     1921 gaccgacgaa caactgaacg agcttctaaa cctcgtgaag aaggtcgaca gcgtcgagtt
     1981 gaaacttacg attccagact cgactcggcg ctccgccgct gccgcactcg atgtcgaccc
     2041 tctgaacgcc cagatccgcc aggtgttctt cttcgacacc atcgacctca ccctgaacaa
     2101 ggccggcgtc gtgctgcggg ccaggcgtat ccagggccgc gatggcgact ccaccgtgaa
     2161 attgcgtccg gtggccccag atgacctctc acccgagctg cgcaagaacc ctgcctttgg
     2221 ggtcgaagtc gacgcccttc ccggtggcta tgtctgctcg ggcacgatga agggagtcgc
     2281 aaccgcggtc gcagttcggg aaacggcggc cggtctgcgt ccgatccgca agttgtttac
     2341 caaggaacag cgggcctttt acgccgctca cgccccggaa gggatcgagc tcgacgacct
     2401 cctgatcctg gggccgatca ccgtgttcaa gctcaagctg gctccacgtg acttcaatcg
     2461 cgggctggtg atcgagcaat ggaactatcc ggacggctcc cgcatcgtcg agttgtcgac
     2521 caaatgccac cccagcgagg ccttccaggt ggcagccgag gcgagggctt acttgagcgg
     2581 gatcgggatt gacctctctg gtgagcagca gaccaagacc gcggccgctc tcgaattctt
     2641 catcaacgag gctcgagccg agaacggcgg aaacaccaag acgaggaaga gccccaccag
     2701 ctagcggctg tttggacttc gcgactctga cttgtaaaca acggcaatga ggcatttcct
     2761 gaccaccgcc gactggtcac gccccgagct gcaagccctc atcgacgacg cccgcagcct
     2821 caagcagagc ccgttcggtg actccctcgc cgggaagacc atcgccctgc tcttcctgaa
     2881 caactccctc cgcacccgaa cctcattcga cgtcgggatc aaccaactcg gtggtcacgc
     2941 tgtcgtgctc tcaccttctg gcgggatgtg gccgctcgag tttgctgatg gagcgatcat
     3001 ggatggcgat gcggaggagc acgtcaagga agcggccaaa gtcctgtccc gctactgcga
     3061 cctgatcggg attcgggcat tccccaagtt ccaggattgg gaagaggacc gtaaggacct
     3121 gatcctcaac tcgttcgcga cctattcgga cgttcccgtc atcaacatgg agacgatcgt
     3181 ccacccctgt caggaactgg cccacatcat ggcgctcgaa gagcaactgg gcgctctcga
     3241 tggcaagaag tacctgctga cctggagtta tcaccccaag ccgctgaaca ccgctgtggc
     3301 caactcggcc ctgacgatcg cggcaaagtt cggcatggag gtaaccctcc tctgtccaac
     3361 tccggactat gtcctggatg agcgctacat ggagcaagct cgagccgatg cgaccgccca
     3421 ggggcaccaa gtgaccatca gccacgacat cgaggaggcc tacgccgacg ccgacgtggt
     3481 gtacgccaag agttggggag cgttgccatt cttcggcaat tgggagagtg agaagccgat
     3541 ccgggacagt tacaggcact tcattgtcga cgaacagaag atggccctga ccaacgacgc
     3601 cctgatcagc cattgccttc cgatgcgacg caacctgatc atgaccgatg ccgtattcga
     3661 tggacccaac tgtttcgcct atgacgaggc cgaagaccgg ctccatgtgc agaaggcgat
     3721 catgacggcg ctgttgggcg acaagaattg acacgacttg caatggcgac caaccgagac
     3781 atccgacaga ccatcgtcca cttgctgagc aacatgagcg acggcaagga gatccgctcg
     3841 tacctgcagc gtttctcgga gctagac
//
LOCUS       DBNHXU010000191         1564 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4623330, whole genome shotgun sequence.
ACCESSION   DBNHXU010000191 DBNHXU010000000
VERSION     DBNHXU010000191.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1564)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1564
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4623330"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1011
                     /gene="thrC"
                     /locus_tag="AC161X_04815"
     CDS             <1..1011
                     /gene="thrC"
                     /locus_tag="AC161X_04815"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HIR4988903.1"
                     /translation="ASRFRHPEIAPVVPVGDRLVFELFWGPTFSFKDHALQVLGGLLE
                     RELGAGTGVILGATSGDTGSAAIEAVRGRPNMRIVVLYPEGMVSDFQRRQMTTVPDEN
                     VSAVAVRGTFDDCQTMVKEAFLRNHDLLAFNSINWARVAVQVGYYVHLGSKFGEPFDV
                     VVPTGNFGNVYSCWLAKQMGTPIASITIANNANRGLSDLVNSGQMSQVPVVATVAPAM
                     DVGLPSNLERFIGDPRVEFTAGAASDAEIRQTIAEVYRDQGYLLDPHSAAAWHVGSAT
                     VAARPQVVVATAHPAKFSATIAESLGQALDWPDLAPGLFDLEERIVVIDADSSALEPF
                     IR"
     gene            1192..>1564
                     /locus_tag="AC161X_04820"
     CDS             1192..>1564
                     /locus_tag="AC161X_04820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988904.1"
                     /translation="MTTRAQLQSKSIDELRQIAEAVGIETDGLQKSKLISALADAGQV
                     AQDGSPADVVELPQVTVRPERSAPEGDSDESEGGSADSEEPRPAPRVDGSSEFSDEFR
                     PGGSRQRRRRRGGSGQRDDEFI"
BASE COUNT          281 a          498 c          493 g          292 t
ORIGIN      
        1 gcatccaggt tccggcaccc cgaaatcgcc cccgtggtcc cggtcggtga tcgcctggtc
       61 ttcgagctgt tttggggacc cactttctcc ttcaaggatc atgctctcca ggttctgggt
      121 ggtctcctcg agcgtgagtt gggggccggg accggagtga tcctcggcgc cacgtccggt
      181 gacaccggat ccgccgccat cgaggcggtt cgaggaaggc ccaacatgag aatcgtcgtt
      241 ctctaccccg aggggatggt gtcggacttc cagcgacgcc agatgaccac ggtccccgat
      301 gagaacgtgt cggcggtcgc cgtccgcggg accttcgacg actgccagac gatggtcaag
      361 gaggcattcc tgaggaacca cgatctgctg gcgttcaact cgatcaattg ggctcgggtc
      421 gcggtccagg tcggctatta cgtccatctc gggtcgaagt ttggagagcc gttcgatgtc
      481 gtggttccca cgggcaactt tggcaacgtc tactcgtgct ggctggccaa gcagatgggg
      541 actccgatcg cgtcgatcac catcgccaac aacgccaatc ggggtctctc cgatctggtc
      601 aacagcggcc agatgagtca ggtgccggtg gtagcgaccg tggccccggc gatggacgtc
      661 ggattaccgt cgaacctgga gcgtttcatc ggtgatcccc gggtcgagtt caccgccgga
      721 gctgccagcg acgctgaaat tcgccagacc atcgccgagg tctaccggga tcagggctat
      781 ctcctcgacc cccacagcgc ggcggcatgg catgtcggct cagcaacggt tgcggcccgg
      841 cctcaggtgg tcgtcgccac cgcccacccc gccaagttct cggcgacgat cgccgagagc
      901 ctgggacagg cgctggattg gccggatctt gctcctggct tgttcgacct cgaggagcga
      961 atagtcgtga tcgacgccga ctcttcagcc ctggagccat tcatccgata gacaggacgg
     1021 acgctgcttt gcgcaatcgc gtcgagtagc tacgatgaca ttgctgccgg cgccaaggga
     1081 cgcggcaacg atccactcaa cagccttctt ccaaggcatt gccccatagc tatgcgatgg
     1141 tgcacatccc ggtccaagtg agcccccaag ggaccaatcg cgaaggatcc aatgaccaca
     1201 cgagctcaac ttcagagcaa gtcgatcgat gaactccgtc agattgcgga ggcggtcggc
     1261 atcgagaccg acggtctcca gaaatcgaaa ttgattagcg ccttggccga cgccggccag
     1321 gtcgcccagg acggctcgcc cgccgatgtc gtcgaactgc cccaggtgac ggtccgccct
     1381 gagcggagcg cgcctgaggg cgattccgat gagagcgagg gcggatcagc cgactcggaa
     1441 gagccgcgcc ctgccccgcg tgtcgacgga tcttctgagt tctccgatga gttccgtccc
     1501 ggcggcagcc gtcaacgccg tcgtcgtcgt ggcggtagcg gtcagcgtga tgacgagttc
     1561 atcc
//
LOCUS       DBNHXU010000192         3301 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_853287, whole genome shotgun sequence.
ACCESSION   DBNHXU010000192 DBNHXU010000000
VERSION     DBNHXU010000192.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3301)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3301
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_853287"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="AC161X_04825"
     CDS             <1..527
                     /locus_tag="AC161X_04825"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988905.1"
                     /translation="TPERFRDIELVFGEKGVARRCFCMHWRRPDGGYPDRRPTRDRFA
                     DRVSEGRPPGLIGYLDQEPVGWVQVGPRDEFPTIGRSRLLKPVDDAETWSINCFVTRV
                     GYRRRGVAGALLVAAKDFARAEGATLVEGYPVDGDRASVVDYFTGTFGMFQEEGFVEI
                     LRRDSTRPIVRAEV"
     gene            complement(610..1515)
                     /locus_tag="AC161X_04830"
     CDS             complement(610..1515)
                     /locus_tag="AC161X_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006242987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988906.1"
                     /translation="MSGGGATRSLRSAVSNPWGKSRLLPFVTMLYVLWSTIPVAIAVL
                     FSFNSGRSRSTWQGFSTRWYVGDPAAAESVLYDQSLRFALAQSVRLAVLTMLVATPIG
                     VALAIGLARWRGRGVKASNLLMLIPLVTPEIVMGVALFLVFDKVYDFVPFGTWTQFLG
                     HVTFSISFVVIIVRGRLFAIGRDYEEAAMDLGASPLQAMRRVLLPLLAPALLASFAIV
                     FAISIDDFVISQFLLGGADTVTIPVQLYSGARAAPPPSLNALATVLMVITIGAVAAAG
                     LLFRALNKRRGEGGSALESVAGFEM"
     gene            complement(1512..2441)
                     /locus_tag="AC161X_04835"
     CDS             complement(1512..2441)
                     /locus_tag="AC161X_04835"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988907.1"
                     /translation="MPTNLASGAEYDTVGQRRWVWPGFALPGVAWLVLLFLVPFYAIF
                     AVAMGQLDPVLQRATPEWNPIYWDLTVFSKVFADLFGGQLGVIALRTLAYVFVASLLC
                     LIIGYPVAYFVSRKAGRWRWLWLLLILAPFWINYLMRMMSWVNLLSPDGQINKWLEAF
                     GITNPPDWLAGSHLVVILGLVYGYIPFFILPLFAALDRIDDATLEAARDLGARPSQTF
                     WRVTWPMSRNGVLGGLVIIMLPMFGDYYTPTLLSGSPRTRMYGNSIDQFINQTTSGGA
                     IGAALTIILMIFVGGLMVYYLRSINRSSQEARE"
     gene            complement(2463..>3301)
                     /locus_tag="AC161X_04840"
     CDS             complement(2463..>3301)
                     /locus_tag="AC161X_04840"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR4988908.1"
                     /translation="QPLNRDYLPNLDVNVWPQLADPYYDKGSRYSVPYVTYTTGIGWR
                     IDLADSADIEGQSNPWDIFWNPKYTGVIGMLESFTDVIPIGLFHNGVADPSKATEAEL
                     VAAGDALVELIDLVDIRYKIDGSYAGLPEGRYAIHQAWSGDLVNSQYYWPGGVDLGTT
                     RYLWPAKSEGSTTHGAISNDTMTVLKGAQHPVLAHQFINFMLDQDNALKNFEFVGYQP
                     PQNGLTSDYLISEGWLLPHLEPALVLPEDFELDSAWVQGPLDAATEALWVEQWNRANA
                     GG"
BASE COUNT          666 a          988 c         1059 g          588 t
ORIGIN      
        1 tgactccgga gcggttccgg gacatcgagc ttgtgttcgg ggagaaaggg gtcgcccggc
       61 gttgcttctg catgcactgg cgtcgacccg acggcgggta cccggaccgc cgtccgacgc
      121 gtgatcgttt cgcggaccgg gtcagtgagg gcaggccacc gggcttgatc ggatacctgg
      181 atcaggaacc ggttggatgg gttcaggtgg gcccccgtga tgagttcccg accatcgggc
      241 gctcccgcct cctcaaaccc gtcgatgacg ccgagacctg gtcgatcaac tgtttcgtga
      301 caagggtcgg ttatcgccgt cgtggtgttg ccggtgcatt gttggtagcg gccaaagact
      361 tcgccagagc cgagggtgca acgctggtgg agggctatcc ggtcgatgga gaccgggcct
      421 cggtggtcga ctacttcacg gggactttcg gcatgttcca agaggagggc tttgtggaga
      481 tcctccgccg ggactcgacc cggccgatcg tgcgagccga ggtttgacgc attgtctcct
      541 cccccgggag cgaagcgacg ttgggggagg tggcccgaag ggccggaggg ggcacttccg
      601 gcgcgtcact cacatttcga agccggccac tgactccagg gcggagcctc cctccccacg
      661 acgcttgttc agcgcccgga acaggagccc ggccgcggcc accgccccga tcgtgatcac
      721 catcagcacc gtggccaatg cattgaggga tggtggtggt gcggcacggg cacctgagta
      781 gagctggacc gggatggtga ccgtgtcggc ccctcccaac aggaactggc tgatgacgaa
      841 gtcgtcgata gaaatggcga agacgatggc gaaactggcc aatagcgccg gagcgagcag
      901 gggtagcaac acacgcctca tcgcctgcag tggagaagcg cctagatcca tggcggcctc
      961 ctcatagtcc ctgccgatgg cgaacagcct cccgcgcacg atgatcacca cgaacgagat
     1021 cgaaaacgtg acgtgtccca agaactgggt ccaggttccg aatggcacga agtcgtacac
     1081 cttgtcgaac accaagaaca gggcgacgcc catcacgatc tccggggtca ccaacgggat
     1141 gagcatcagc agattggatg ccttcacccc ccgccctcgc catctcgcca acccaatggc
     1201 gagagctacg ccgatcggcg tcgccaccaa catcgtcagc actgccagcc ggacgctctg
     1261 ggcgagggcg aatcgcagtg actggtcgta gagcactgat tcggcggctg cagggtcacc
     1321 gacgtaccag cgagtggaga atccctgcca ggtactcctt gagcgaccgg agttgaagga
     1381 gaagagaacg gcaatcgcaa ccggaatcgt cgaccagagg acatagagca tggtcacgaa
     1441 gggcaggagc ctcgactttc cccatgggtt gctgacagcc gacctgagcg accgggtggc
     1501 accgcctccg ctcattcgcg agcctcctgc gaggagcggt tgatcgaccg caggtagtac
     1561 accatcaggc ctcccacgaa gatcatcagg atgatggtca acgcagcccc gatggccccg
     1621 ccactggtgg tctggttgat gaactggtcg atcgagttcc cgtacatgcg agtgcgtggc
     1681 gagcccgaca ggagagtcgg cgtgtagtag tcgccgaaca tcgggagcat gatgatcacc
     1741 aggccgccga gcactccgtt ccgtgacatc ggccaggtga cccgccagaa ggtttgcgac
     1801 ggccttgccc cgaggtcacg tgcggcttcc aaggtggcat cgtcgatccg gtccagcgcg
     1861 gcgaacaggg ggaggatgaa aaacgggatg tatccgtaga ccaggccgag gatgaccaca
     1921 agatggctgc ctgccagcca atcgggagga ttggtgatgc cgaatgcctc cagccacttg
     1981 ttgatctggc catcagggct caacagattc acccacgaca tcatccgcat cagatagttg
     2041 atccaaaacg gggccaggat cagcaacaac cacagccagc gccaccgacc ggccttccgg
     2101 gatacgaagt aggcgaccgg gtagccgatg atcaggcaca aaagcgaggc gacgaacacg
     2161 taggcgagtg tccgcagtgc gatcactccg agctggccac cgaacaggtc cgcgaacacc
     2221 ttcgagaaca cggtgaggtc ccagtagatc gggttccact caggagtcgc tcgctgcagc
     2281 accgggtcga gttggcccat tgccactgcg aatattgcgt agaacggaac caggaacaag
     2341 aggaccagcc aggccactcc gggaagggcg aaaccgggcc atacccatcg tctctgcccg
     2401 acggtgtcgt actcggcgcc cgaggcgagg ttggtgggca acctttcccc taggtcacag
     2461 gctcagccac cggcgttggc ccgattccat tgttcgaccc acaacgcctc ggttgccgca
     2521 tcgagtggcc cctggaccca ggcactgtcc aattcgaagt cctcgggcag taccaaagcg
     2581 ggttcgaggt gcggcagcag ccacccctcg ctgatcaagt aatcggaggt gagaccgttt
     2641 tgcggtggct gatatcccac gaattcgaag ttcttgagcg cattgtcctg atcgagcatg
     2701 aagttgatga actggtgggc caggaccggg tgttgggccc ctttgaggac ggtcatggtg
     2761 tcgttggaga ttgccccgtg cgtggtcgat ccttccgact ttgccggcca taggtaccgg
     2821 gtcgtgccga gatcgacccc gcctggccag tagtactggg agttgacgag gtccccggac
     2881 cacgcttggt ggattgcgta ccgaccctcc ggcagaccgg cgtatgaacc gtcgatcttg
     2941 tatcggatgt cgaccaggtc gatcaattcg acgagcgcgt caccggcggc gaccagttcg
     3001 gcttcagtgg ctttcgatgg atctgcgact ccattatgga ataggccgat cggtattacg
     3061 tcggtgaagg actcaagcat cccgatcacc ccggtgtact tcgggttcca gaagatatcc
     3121 catgggttcg actggccctc gatgtcggca ctgtctgcca gatcgatccg ccaaccgatg
     3181 ccggtcgtgt atgtcacata tggcaccgag tatcgggagc ccttgtcgta ataagggtcg
     3241 gccagttggg gccagacgtt cacgtcgagg ttcggcaggt agtcgcgatt cagcggctgg
     3301 a
//
LOCUS       DBNHXU010000193         3293 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2638888, whole genome shotgun sequence.
ACCESSION   DBNHXU010000193 DBNHXU010000000
VERSION     DBNHXU010000193.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2638888"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1143
                     /gene="cimA"
                     /locus_tag="AC161X_04845"
     CDS             <1..1143
                     /gene="cimA"
                     /locus_tag="AC161X_04845"
                     /inference="COORDINATES: protein motif:HMM:TIGR00977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043714 - (R)-citramalate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046912 - acyltransferase activity, acyl
                     groups converted into alkyl on transfer [Evidence IEA];
                     GO_process: GO:0019752 - carboxylic acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:1901705 - L-isoleucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR4988909.1"
                     /translation="FFDAEHFFDGFRSNPDYALSVLETAARAGAERVVLCDTNGGALP
                     SHIIPALDAVKARIETKLGVHFHNDSDCAVANSMTALAHGVYQVQGCVNGYGERTGNA
                     DLCSVIPNLSLKSGVETIPWESVARLYPIAQQIAEILGAPIDSRRPYVGASAFAHKAG
                     LHTSGLARLEGAYEHIDPGAVGNSARMLFSELMGRSTVLAVAEEKGWDLDAEKAQALV
                     DQVKELEHAGYQFEAADGSFEMLMRRASEDFEELYEVRSMSVSIQTNEAGKVTARAHL
                     VVAVEDDEIEVSTEGDGPVNAMDEALRKALEAAYPDVKRLRLTDYKVRDLDSSDGTAA
                     RVRVLIETTDGDSTWGTVGVHQNIIEASWQALREGITVGLLRAGDA"
     gene            complement(1109..1681)
                     /locus_tag="AC161X_04850"
     CDS             complement(1109..1681)
                     /locus_tag="AC161X_04850"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF013718.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HIR4988910.1"
                     /translation="MTQQPNTVDLLLDLDETIFRQVADWKSPILDGTLPSLSEIASYS
                     RIWIGVSLGLTAFGGAKGRRSAVLGMAAIGVTSALTNIALKGVTNRKRPQREVPAARR
                     LVHPESTSFPSGHTASAAAFSGVVGREMPGLWLPLNALAGLVGFSRIYTGVHYPGDVL
                     AGWLVGKLVAAMVRWVSRRFRRLQPATVQP"
     gene            1808..3106
                     /locus_tag="AC161X_04855"
     CDS             1808..3106
                     /locus_tag="AC161X_04855"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006335359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR4988911.1"
                     /translation="MILAVTAVLVLALVRNLPSMIFGLPGLAIMVGAAWWIITETGLR
                     RILGGVGSAIGALLIVAALMVTYHRPEQFILRLVIVIGALTLAMMMARYSVTRTVPET
                     AKGKAPHLSRGLNHPVLLCNPWSGGGKVESFGLVGLAKELGIETVMLEPGLDLEELAL
                     DAVARGADCLGMAGGDGSQALVASIAIKHDLPFVCVSAGTRNHFALDLGLDRDDPRPS
                     MRAFHDMVERKVDFATVNGRLFVNNVSLGIYATIVQSEDYRENKSEVSMSMAAELLGH
                     QGEGFDLQYTTSDGDRIEDAFLIMVSNNPYVLGIKRDVAQRRQMDGGVLGVFAVSTRT
                     AAQAAHLLTMSAIGLRKASPHWHEFTTDEFEVRSHVGAAFVGVDGEAFQSSTPLKFAS
                     HPRGLRMYVPEGNLEAAERRRARDFRIQDVAAVAMGHTSW"
     gene            3197..3286
                     /locus_tag="AC161X_04860"
     CDS             3197..3286
                     /locus_tag="AC161X_04860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_369335557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein bS22"
                     /protein_id="HIR4988912.1"
                     /translation="MGSLVKKRRKKMSKHKYRKRLKANRHKRK"
BASE COUNT          608 a          991 c         1083 g          611 t
ORIGIN      
        1 ttcttcgacg ccgagcactt cttcgacggg ttccgctcca atcccgatta cgccctatcg
       61 gtgctggaga ccgcggccag ggccggggcc gagagagttg tgctctgtga caccaacggg
      121 ggagccctcc cctctcacat catccccgcc ctggacgcgg tgaaagcgcg gatcgagacc
      181 aagctcggag tccacttcca caacgactcc gactgtgcgg tcgccaactc gatgacagcg
      241 ctggcccatg gcgtgtatca ggttcagggc tgcgtcaacg gctacggaga gcggaccggg
      301 aacgccgacc tctgctcggt catccccaac ctcagcctga agtcgggggt ggagaccatc
      361 ccctgggagt cggtggcccg cctctacccg atcgcccagc agatcgccga gatcctcggc
      421 gcccccatcg attcccgtcg gccctatgtc ggcgcctccg cctttgccca caaggcgggc
      481 ctccacactt cggggttggc ccgtctcgag ggcgcctacg agcacatcga tccgggggca
      541 gtcggcaatt cggcccgcat gctgttcagt gagttgatgg gccgttcgac ggtgttggcc
      601 gtggccgagg agaaagggtg ggatttggac gccgagaaag cccaggcgtt ggtcgatcag
      661 gtcaaagagc tcgagcacgc cggctatcag ttcgaggcgg cggacggttc attcgagatg
      721 ctgatgcgcc gggccagcga ggacttcgag gagctgtacg aggtccggtc gatgtcggtg
      781 agcatccaga ccaatgaggc gggcaaggtg acggcgcggg cccatctggt ggtggcggtg
      841 gaagacgacg agatcgaggt gtcgaccgag ggtgacggcc cggtcaacgc catggatgag
      901 gcgctgcgca aggcgttgga ggccgcctac cccgatgtga agcgcctccg gctgaccgac
      961 tacaaggtcc gcgacctcga ctcatccgac gggaccgcgg ctcgggtgcg ggtcttgatc
     1021 gagactactg acggcgattc gacgtgggga acggtcgggg tccaccagaa cataatcgag
     1081 gcctcctggc aggcgttgcg cgaagggatc acggttggac tgttgcgggc tggagacgcc
     1141 taaaccgtcg ggacacccat cggaccatcg ccgccaccaa ctttcccacg agccacccag
     1201 cgaggacgtc gcccggatag tgaaccccgg tgtagatccg tgagaacccg acgaggccag
     1261 ccaatgcgtt caggggcagc catagacccg gcatctcacg tcccaccact cccgagaaag
     1321 ctgcggccga ggcggtgtga ccggagggaa aggacgttga ctccggatgg actagacgtc
     1381 gcgccgcagg aacctcacgc tgcggacgtt tccgattcgt tacgcccttg agggcgatgt
     1441 tggtcagtgc ggatgtgaca ccgattgcgg ccattcccag caccgcactt cgtcggccct
     1501 tcgcaccgcc gaaggcggtg aggcccaagg acacaccaat ccagatccgg gagtagctgg
     1561 cgatctcgga cagactcggg agggtcccat cgaggatcgg ggacttccaa tcggcgacct
     1621 gtctgaagat cgtctcgtcg aggtcgagaa gaagatctac ggtgttcggc tgctgcgtca
     1681 tcgctggcgc atgatggcag cgtcaccgtc gggtcgcctc cgcctcacat tcggactgct
     1741 gcgtattctc cgtttcatgg agcggcccgg tgccaagcag cgaatagctg ccatagcttt
     1801 tctccccatg atcttggctg tcacggcggt attggtcctc gctctggtcc ggaatctgcc
     1861 gagcatgatc ttcggcttgc cgggtctggc aatcatggtc ggcgcagcgt ggtggatcat
     1921 cactgagacc gggctgaggc ggatccttgg tggagtgggc agcgcaatag gtgccttgtt
     1981 gatcgtcgct gcactcatgg tcacctacca tcggccggag cagttcatcc tgcggctggt
     2041 gatcgttatc ggagccctaa cgctggccat gatgatggcc cgctattcgg tgacccggac
     2101 ggtgccggag accgccaaag ggaaggcgcc acatctttcc cgagggctga accatccggt
     2161 ccttctctgc aatccgtggt cgggcggtgg gaaggtcgag agtttcgggt tggtgggatt
     2221 ggccaaggag ctcggcattg agaccgtgat gctggagcca ggtctcgacc tcgaggagct
     2281 tgcattggac gccgtcgccc gcggtgccga ctgcctgggc atggccggcg gggacggctc
     2341 ccaggccctg gttgcgtcga tcgcgatcaa gcacgacctg cccttcgttt gcgtgtcggc
     2401 gggaacccgc aaccacttcg cactcgacct cggcctggat cgcgacgatc cacggccgag
     2461 catgagggcc tttcacgaca tggtggagag gaaagtggac ttcgccacgg tcaacggtcg
     2521 gctattcgtc aacaacgtct cgctcggtat ctacgcaacc atcgtccaat cggaggacta
     2581 tcgggagaac aagagcgagg tgagcatgtc gatggcagcc gagctcctcg gccaccaggg
     2641 ggaaggcttc gacctccagt acacgacgtc ggatggagac aggatcgagg acgcatttct
     2701 gataatggtg tccaacaacc cttatgtgct tggtatcaag cgcgacgtgg cgcaacgtcg
     2761 gcagatggat gggggtgttc tcggagtgtt cgctgtctcg acgagaacgg cagcacaggc
     2821 ggcccatctg ctcaccatgt ccgccatcgg tttgcggaag gccagccccc actggcatga
     2881 gttcacaacg gacgagttcg aggtgcgatc tcacgtcggc gccgcgttcg tgggcgtcga
     2941 tggtgaggca tttcaatctt cgacacctct gaagtttgcg agccaccccc gcggactcag
     3001 gatgtacgtg cccgaaggca acctggaggc ggcagagagg cgccgggctc gagactttcg
     3061 catccaggac gttgccgccg tggccatggg gcacaccagc tggtagatcg gggatgtgaa
     3121 ccctgctccg gtttgaccta gttgccggat cacgcgacca ttgactcgtc gtcccccttc
     3181 tccgtgaggt ttttgcatgg gatcgttggt caaaaagcgt cgtaagaaga tgtcgaagca
     3241 caagtaccgc aagcgtctga aggcgaatcg ccacaagcgg aagtgacttc gtg
//
LOCUS       DBNHXU010000194         6029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1452361, whole genome shotgun sequence.
ACCESSION   DBNHXU010000194 DBNHXU010000000
VERSION     DBNHXU010000194.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6029
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1452361"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="AC161X_04865"
     CDS             <1..1046
                     /locus_tag="AC161X_04865"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009101 - glycoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HIR4988913.1"
                     /translation="VPDLEAKGIRHIALTSSTRGMNLLADLRAIGQFWRILRQERPDI
                     LHTHNPKPGIYGRIVGRLAGVPIVVNTIHGLYATPESSFPKRAVVYTLEWVASRFSDA
                     ELIQSPEDFELLWQRKIMPRSKLRLLGNGVDLERFKPDPLLRQQVRQELGLKPDQVAV
                     GLVARLVEEKGVPELIEAAKRLDSRYSVFIVGPKDHEKSDAVSEELLTEAEANGIRFL
                     GMRKDVERLYQAFDVFVLPSHREGFPRAAMEAAASGLPVIATDIRGCRQVVDHGVNGY
                     LFPVGDVDALARAITEVGEEPELRAAMSQSSVERAHVLFDERDIVNRVMGSYAEVASR
                     KGIYPALVERWGG"
     gene            complement(1054..1827)
                     /locus_tag="AC161X_04870"
     CDS             complement(1054..1827)
                     /locus_tag="AC161X_04870"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: protein motif:HMM:NF012760.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0071951 - conversion of methionyl-tRNA to
                     N-formyl-methionyl-tRNA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HIR4988914.1"
                     /translation="MIRFAFAGDRQSAVSCLSILLETDAHPEALLVSGPDRATHDEVL
                     REMSGLPSDRVLVGREFREPAGIELLGSLDLDYVIGVHFPYIVPDEVLAIPRIGVLNL
                     HPALLPYNRGWHTPSWAILDGTPIGATLHFMDSGLDTGDIVAQVELEIRPEDTAHTLY
                     SRLLQLEVDLFRQAWPLLASGSPPRQAQSAAEGSSHNRVDLASDSVRRLDLEASVPVK
                     DLLRTLRALTTNDRGEAAYFEVDGRRYTVQVNITPDADK"
     gene            complement(1824..2414)
                     /locus_tag="AC161X_04875"
     CDS             complement(1824..2414)
                     /locus_tag="AC161X_04875"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018124418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR4988915.1"
                     /translation="MKRGLDFTISALALVILSPVLLVIGALVWLKLGLPVLFTQQRPG
                     LHGRPFTLVKFRTMRVAPADPDGSSDADRLEPFGAKLRSTSLDELPELWNVLTGDMSL
                     VGPRPLLMHYLPLYSERQARRHEVRPGITGWAQINGRNSTPWPERLEMDVWYVENRST
                     WLDLKILFMTIPSALKRSGVAQEGQATVEYFTGSET"
     gene            complement(2411..3769)
                     /locus_tag="AC161X_04880"
     CDS             complement(2411..3769)
                     /locus_tag="AC161X_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988916.1"
                     /translation="MNSEGWFWVATGLSAVGLGLVVALGASGAGVLLIAILGTVLMAG
                     LASFMRRPVDAAWLTRWVTLGFIAKILGSAARYYMVQLYESGDSLSYYAAGTQLAAIW
                     RSGSVPALADGGGGGFGTQVVESFTGGLFAIFTPDMLGGFVIFSMLSFMGQLGLYAAF
                     RRWAQPHQLKPYAFLVLFLPTYAFWPSSIGKDALVILCLGLAAYFIARLLESFELRWI
                     LPLAVSLTGLGLIRIHIAALVALALVGALLVSKLRIGAGFLARGRKFITFFGAAAAVV
                     LAVTLIPDIVGLELGDLQDVSPFTAEITRRTSEDGTVAAGDPVSSISDVPGAVALVLF
                     RPYIFEATELQHFLAAAETTLILLLTLWKLPAMLRNWRGWRNNAYLVFCTLYTLAFAV
                     AFSVVRNLGIIARQRGQVLAFFLAVVIGLGWSETKKVTRARIPVPPLEPEPDLAGTAV
                     HR"
     gene            complement(3766..4713)
                     /locus_tag="AC161X_04885"
     CDS             complement(3766..4713)
                     /locus_tag="AC161X_04885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988917.1"
                     /translation="MSEMEIRPARPEDVPAILDVLGAALGETALLKRTPEQWAWKHEH
                     NPFGRSTVLIAWIAGRIAGVRAFMRWDLLSPTGHVVRCVRPVDTATHPDFERRGVFKA
                     LTLEAVDLARNEGIDLIFNTPNPRSGAGYMTMGWQEVGAIGVLVRPRFGASVPTDAGG
                     IPDLDEVAPGAIAFRPIGGQDRQPRGLRTPRTEDYQLWRFGSHPTVRYATVNDGAGAA
                     IVRVGVRNGRTELVLSDLLFGAGRRAVSKAGAANRGAYLAGFFSKGSPERAAAIQGRM
                     FPVPGIKTLQLVANPLTELDTDPFDLASWDIATSDLELL"
     gene            complement(4706..5818)
                     /locus_tag="AC161X_04890"
     CDS             complement(4706..5818)
                     /locus_tag="AC161X_04890"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4988918.1"
                     /translation="MVSSTPGERHRILVLIKGLGIGGAERLIAEGATYWDRDQFDYRV
                     AYVLPWKDQLVAEIEGHLVSVDCVGSDRGLGPTAYRKLRSLVSKWDPDLIHAHLPSAG
                     ILARIATRRPVIYTEHNIASSYRQPTRSVNRLTYGRNASVIAVSEAVAEGLAGYPGPT
                     PRVIPNGVSVSVSPDDIEAVRTELGIQADRSLVVHVGNIRPHKGHENLIAATALLAAQ
                     DPDVLVVSVGAEKHDGDLARVRASAESAGVSDQIRFMGRREDARSFLAAADLVVNPAD
                     VEGLPLAILEALALSRPVVATAVGGVPTVVVNHVTGLLVPPGDPLALATAMRTALDSP
                     DAKAWAQAGAELVSTQYGIARMITDYEKLYREVLHV"
     gene            complement(5796..>6029)
                     /locus_tag="AC161X_04895"
     CDS             complement(5796..>6029)
                     /locus_tag="AC161X_04895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988919.1"
                     /translation="SGMAINTVASESVTIRELAEKIVQRFPTELTFGEPRPGDVPPAA
                     VSAKRAEEVLGWRSEMPFDQGLTELIDGLVDAG"
BASE COUNT         1192 a         1857 c         1945 g         1035 t
ORIGIN      
        1 cggttcccga cctcgaggcg aaaggcatcc gccatatcgc cctcacctcg tcgacccgtg
       61 ggatgaatct gttggctgat ctacgggcga tcggtcagtt ctggagaatt ctgagacagg
      121 agcgccctga catcctccac acccacaacc ccaagcccgg tatctacggg cggatcgtcg
      181 gcaggttggc cggagtgcca atcgtggtga acaccattca tggcctctat gcgacgccag
      241 agtcgtcttt cccgaagcga gccgtcgtct acacgttgga atgggtggcc tcccggttct
      301 ccgatgccga actgatccag agccccgagg atttcgagtt gttgtggcag cgaaagatca
      361 tgccccgctc caagcttcgc ctgttgggga acggggtcga tctcgaaaga ttcaagcccg
      421 atccgctctt gaggcagcaa gtgagacagg agctgggcct gaaacccgac caggtcgcgg
      481 ttggcctggt tgcccgactc gtcgaggaga aaggcgtgcc cgagttgatc gaggcggcca
      541 agcgtctcga cagccggtac tcagtgttca tagtcgggcc gaaagaccac gagaagtcag
      601 atgcggtctc cgaggagctt ctcaccgagg ccgaggccaa cgggatccgg ttcctcggca
      661 tgcggaagga cgtggagcgg ctttatcagg cattcgacgt gttcgtcctc cccagtcatc
      721 gagaaggctt cccccgagcg gcgatggagg ctgccgcctc cgggctcccg gtgatcgcca
      781 ccgacatccg tggctgccga caagtggtcg atcatggggt caacggctat ctcttcccgg
      841 tgggggacgt agacgctctg gctagagcga tcaccgaggt gggcgaggag ccagagctga
      901 gagctgccat gtcccaatca tcggtcgaac gggcccacgt ccttttcgat gaacgtgaca
      961 tcgtcaaccg tgtgatgggc tcctacgccg aagtggccag tcgaaaaggg atctatccgg
     1021 ccctcgtcga gcgttggggc ggttagccag cggtcatttg tcggcgtccg gggtgatgtt
     1081 gacctggacc gtgtagcgcc gaccatcgac ctcgaaatac gcagcctccc cccggtcgtt
     1141 tgtggtgaga gctcgaaggg ttcggagaag gtccttgact ggcaccgacg cctcgaggtc
     1201 aagtcgccgt accgaatcag aggcgagatc cacccgattg tgacttgatc cctcagctgc
     1261 cgactgtgcc tgacggggtg ggctccccga ggcgaggaga ggccacgcct gccggaacaa
     1321 gtcgacctcg agctgaagga gtcggctgta cagggtgtgc gcggtgtctt ccgggcgaat
     1381 ctcgagctcg acctgggcga cgatgtcccc ggtgtcgagc ccgctgtcca tgaaatgaag
     1441 ggtggcgcca atcggcgtcc catcgaggat cgcccaactt ggggtgtgcc aaccacggtt
     1501 gtagggcagc aaggcgggat gcaggttgag gacgccgatc cgggggatgg cgagcacctc
     1561 gtcgggcacg atgtatggga agtggacccc gatcacgtag tccaggtcga gggatccgag
     1621 cagttcgatg ccggcgggct cgcggaactc cctaccgacg aggacgcggt ctgagggcag
     1681 tccgctcatc tcacggagga cctcgtcatg ggtggctcga tccggcccgg agaccaggag
     1741 ggcctcagga tgagcgtcgg tctcgaggag gatggagaga cagctcaccg cagattggcg
     1801 atccccggca aatgcgaagc gaatcatgtc tcagaccctg tgaagtattc aacggtcgcc
     1861 tgcccctcct gggccacccc gctccgtttg agggcactgg gaatggtcat gaagaggatc
     1921 ttcaggtcga gccacgtcga gcggttctcc acgtaccaca cgtccatctc gagtcgctcc
     1981 ggccagggtg tcgagttacg tccgttgatc tgcgcccaac cggtgatgcc aggtctgact
     2041 tcgtgacggc gagcctgacg ctctgaatag agcggcaggt agtgcatgag gagaggcctc
     2101 gggccaacca ggctcatatc gccggtgagg acattccata gctccggaag ctcatcgagg
     2161 ctggtgcttc gcagctttgc cccaaagggc tcaaggcggt cggcgtcgga gctgccgtcc
     2221 ggatccgccg gcgccacgcg catggtgcgg aacttgacca gggtgaacgg acggccgtga
     2281 agtcctggtc tctgctgtgt gaagagaacg ggaagcccaa gcttcaacca gaccaaggcc
     2341 ccgatcacaa gcaacacggg cgacaggatc accagcgcca gcgctgagat cgtgaagtcc
     2401 aaacctcgtt tcaccgatgg actgctgtcc cggccaggtc aggctcgggc tccagtggag
     2461 gcaccggtat gcgggcgcgg gtgaccttct tggtctcaga ccatccgagc ccgatgacga
     2521 cagcaaggaa gaaggcgagg acctgtcccc tctgtcgggc gatgatcccc aggttgcgca
     2581 cgacggagaa ggcaacggcg aaggcgagcg tgtagagcgt gcagaaaacg aggtaggcgt
     2641 tatttcgcca cccccgccag ttacgcagca tcgcagggag cttccacagt gtcagcagga
     2701 gaatgagggt cgtctcggcg gccgccagga agtgctggag ctcggtggct tcgaagatgt
     2761 aggggcggaa gaggaccaaa gccaccgcgc cgggcacgtc cgagatcgac gacaccgggt
     2821 caccggcagc caccgtgccg tcctccgagg tgcgccgggt tatctccgcc gtgaatggac
     2881 tgacatcctg gagatcgccc aattccaagc caacgatgtc tggtatgagc gtcaccgcga
     2941 ggaccacggc agctgcggcg ccgaagaatg tgatgaattt cctacctcgg gcgaggaagc
     3001 cggccccgat cctcagtttg gatacgagca acgctcccac gagggcaagg gccaccaatg
     3061 cggcgatgtg gatccggatc aagcccagcc cggtcagcga caccgccagg ggcaggatcc
     3121 aacgcaactc gaagctctcg agcagacgcg ctatgaagta ggcggccagc ccgagacaga
     3181 gaatcaccaa tgcatccttc ccgatgctcg acggccagaa ggcgtaggtg ggcagaaaaa
     3241 ggaccaggaa ggcatatggc ttgagctgat gaggttgggc ccagcggcgg aaagccgcgt
     3301 agagcccgag ctgtcccatg aaggagagca tgctgaatat gacgaaaccg ccgagcatgt
     3361 ccggggtgaa gatggcgaac aggccgccgg tgaaggactc gaccacctgg gtcccgaagc
     3421 cgccccctcc gccatcggcc agggcgggga ccgatccgga tctccagatc gcagccaact
     3481 gggtccctgc ggcgtaatag ctgagggagt ctcccgactc gtagagctgg accatgtaat
     3541 acctggcggc cgagccaagg atcttggcta tgaacccgag ggtgacccat ctcgtcaacc
     3601 atgcggcgtc gacgggccgc ctcatgaaag aggcgagacc ggccatcagc accgtgccca
     3661 ggatggcaat gagcagcaca cctgcccctg aggctcccaa tgcgaccacc aaaccaagcc
     3721 cgacggcgga caggccggtc gcaacccaga accagccctc gctgttcaca acaactccaa
     3781 atcactggtg gcgatgtccc aggaggctag atcgaacgga tcagtatcga gttcggttag
     3841 ggggttggcg accagctgaa gtgtcttgat cccgggtacc ggaaacatcc ttccctggat
     3901 tgccgcagcg cgctcggggg agcccttgga gaagaagccg gcgaggtaag cgccacgatt
     3961 ggcggcgccc gctttggaga ccgcgcgccg gcccgcacca aacaggaggt cggacagaac
     4021 cagctcggtg cgcccgtttc tgacgccaac cctgacgatc gccgctccgg ccccgtcgtt
     4081 gaccgttgcg taccgcaccg tcggatgtga tccgaaacgc cacaactgat agtcctccgt
     4141 ccgcggtgtt cgtaacccgc gtggctggcg gtcctgcccc ccaatcggcc ggaacgcgat
     4201 cgcacctggt gccacctcgt cgaggtcagg gattcccccc gcgtcggttg gaaccgaggc
     4261 accgaaccgg ggacgtacca gcacgccgat ggcaccgacc tcctgccatc ccatcgtcat
     4321 gtatcccgcg ccggaacgtg ggttcggggt gttgaagatg aggtcgattc cctcgtttct
     4381 ggccaggtcc acggcttcca acgtcaaagc cttgaatacc ccccgacgct cgaagtcagg
     4441 gtgggtggcg gtgtccacgg gtctcacgca tctcactaca tgcccggtcg ggctgaggag
     4501 gtcccatcgc atgaaggcgc gcactccagc tatccggccg gcgatccagg caatgagcac
     4561 ggtcgaacga ccgaaggggt tgtgttcgtg cttccaggcc cactgttcgg gtgttcgttt
     4621 gaggagagcg gtctccccaa gtgcggctcc gagaacgtcc aagattgctg gaacgtcttc
     4681 ggggcgggcc gggcggatct ccatctcaga catggagcac ttcccgatac agcttttcgt
     4741 agtcggtgat catgcgagcg atcccgtatt gggtggaaac cagctcggcg cctgcctgcg
     4801 cccaggcctt ggcatcagga gagtcgaggg cagtccgcat ggcggttgcc agcgccaggg
     4861 ggtcaccggg aggcacgaga agcccggtta catggtttac caccacagtg ggaactcctc
     4921 ctacggcagt ggccaccacc ggtcggctca gtgcaagggc ttcgaggatg gccagcggca
     4981 ggccctcgac gtcggccgga ttgaccacca ggtcggcggc tgcaagaaag ctcctggcgt
     5041 cctcacgccg gcccatgaag cgaatctggt ccgacacgcc tgcggattcc gctgacgccc
     5101 ggactcgggc aaggtcgccg tcgtgcttct cggctccgac agacaccacc agcacatcgg
     5161 gatcttgcgc cgccagaagc gctgtcgccg caatcagatt ctcgtggcct ttgtgggggc
     5221 ggatattgcc gacgtgaact acgagagacc ggtccgcctg aatgccgagc tcggttcgaa
     5281 ccgcttcgat gtcgtcaggg ctgaccgaga cggaaacccc atttggtatg actcttggtg
     5341 ttggcccggg ataaccggcc aggccctcgg ccacagcctc ggaaactgca atcactgacg
     5401 cgttgcgtcc ataggtgagg cggttgaccg atcgagtcgg ctgccgataa gagctagcga
     5461 tgttgtgctc cgtgtagatc accggccgcc tcgtcgcaat gcgggccagg atcccggcgc
     5521 tcgggaggtg cgcatgaatc agatcgggat cccatttcga gaccagagat cgaagcttgc
     5581 gatacgcagt ggggccaagg ccgcggtccg agccgacaca gtccacgctg accaagtgcc
     5641 cttcgatctc cgcaaccaac tgatctttcc agggaaggac gtaggccacg cgatagtcga
     5701 actgatcgcg gtcccagtag gtagccccct cggcgatcag gcgctcggcg cctccgattc
     5761 ccaacccctt gatcaggacc agaatccggt gccgttcacc cggcgtcgac gagaccatcg
     5821 atcaattctg tgagaccctg gtcgaaaggc atctccgagc gccacccgag cacctcctcg
     5881 gcacgcttgg cagagaccgc tgcgggggga acatcaccag gtcggggctc accaaatgtc
     5941 agctcggtcg gaaaacgttg cacgatcttt tcggccagtt ccctgatggt gacggactcg
     6001 gaggcgaccg tgttgatggc catacccga
//
LOCUS       DBNHXU010000195         2372 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2840773, whole genome shotgun sequence.
ACCESSION   DBNHXU010000195 DBNHXU010000000
VERSION     DBNHXU010000195.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2840773"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1076
                     /gene="dnaJ"
                     /locus_tag="AC161X_04900"
     CDS             <1..1076
                     /gene="dnaJ"
                     /locus_tag="AC161X_04900"
                     /inference="COORDINATES: protein motif:HMM:NF008035.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR4988920.1"
                     /translation="MARDYYSILGVGRDATTEEIKKAFRRLARATHPDANPGDADAEA
                     RFREAAEAYEVLVDPERRRRYDRGDTIDLSDIFSGFGGFDDILRSVFGDGGLFGSQSS
                     RSSRGRDVLARVEVDLASAAFGTDVSVSFHSRATCPNCAGTGAEPNTGQSTCPECGGS
                     GSVRMARRSLFGTMMTVGACPTCRGEGVLILDPCLICSGDGAVPEETTVTIEVPAGVA
                     SGTRLRLSGRGESGGRSGPPGDLYVEVMVAPDPRFERVESDLIHRVALGIAEATLGSR
                     VEVPLLEGGATDLDIPPATQPGTVFQLTGLGVPRLGRRARGDLRVVVEVRIPDELSAE
                     EESLLRQWSDLRGERTDRPASAG"
     gene            1076..1732
                     /locus_tag="AC161X_04905"
     CDS             1076..1732
                     /locus_tag="AC161X_04905"
                     /inference="COORDINATES: protein motif:HMM:NF016347.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HIR4988921.1"
                     /translation="MASHYPHVLAMSDWDDATVQLTNDQIHHLERVLRLKPGEAVGYT
                     DGKGTVGRGVYEGGRINRDQEGTVPRPSPLTMAVPPPASRERCRFLVEKLAELGVARL
                     VWLSTRFGEGHVPHRTKAMSWAVSALEQSRGGWLMEIGEGLVAWEDLHRPLVVAVPGG
                     GSEVFEVMTVAIGPEGGLDPAEIPADAATIDLGVTILRVETAAVVAATRFSRPWHGGS
                     "
     gene            1774..2187
                     /locus_tag="AC161X_04910"
     CDS             1774..2187
                     /locus_tag="AC161X_04910"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4988922.1"
                     /translation="MVDKFSASLGVRLRAARRQRGWSLGEVESFTDGEFKASVVGAYE
                     RGERAISVQRLTRLAEVYGVGPTELFPGTKPSDGPVIDIDAVAAGRDHDMVDRYLAAI
                     QFMRREGTPGEVRESDKAVISSLLETSIDAVSETD"
BASE COUNT          413 a          690 c          806 g          463 t
ORIGIN      
        1 agatggcgag ggattactac tcaatactcg gggtcggtag ggacgcgacc accgaggaga
       61 tcaagaaggc atttcgccgt ctggcgcggg ctacgcaccc cgacgccaat ccgggcgatg
      121 ccgatgcgga agcaaggttt cgagaggctg ccgaggcgta cgaggtgttg gtcgatcccg
      181 agcggcgcag acgttacgac cgcggcgaca ccattgacct ttccgacatc ttctccggat
      241 ttggtgggtt cgacgacatt ctccgttctg tcttcggtga tggggggctg tttggatccc
      301 agtccagccg gtcgagtcgg gggagggatg tactggcccg tgtcgaagtc gatctggcat
      361 cggcggcctt cggcaccgat gtctcggtgt cgtttcacag cagggcgacc tgcccgaact
      421 gtgcgggaac aggtgccgag ccgaataccg gacagtcgac ttgtcccgaa tgtggcggca
      481 gcgggtcggt gcgaatggca cgccgatccc tcttcgggac gatgatgacg gttggagcct
      541 gccccacctg ccgcggtgaa ggagtgctga tcctcgatcc ctgcttgatc tgctcaggag
      601 atggcgcggt gcccgaggaa acgacggtga caatcgaagt gcctgccggg gtcgcctcgg
      661 gcaccaggct ccgactgtcg ggccggggcg agtccggcgg ccgttccggg cctcccggcg
      721 acctatatgt cgaggtcatg gtcgcccctg atccccgttt cgagcgcgtt gagtcagatc
      781 tcatccatcg agtggccttg ggcatcgccg aagcgactct gggctccaga gtcgaagtcc
      841 cgctcctcga gggtggagct accgatctag atattcctcc ggccactcag cccggaacgg
      901 tctttcagct gaccggactt ggcgtgcccc gtctcgggcg aagggctcgg ggagacctgc
      961 gggtggtggt cgaggtgagg atccccgacg agctcagcgc cgaggaagaa tcgctgcttc
     1021 gtcaatggtc cgatctgcgg ggtgagcgca ccgatcgccc ggcatcggca ggctgatggc
     1081 cagtcactac ccacatgtcc tggcgatgag tgattgggac gatgccacgg tccaactcac
     1141 caacgatcaa atccatcatc tcgagcgggt tctgcgattg aagcccggtg aagcagtcgg
     1201 ctacacggac ggcaagggca cggttgggcg cggggtctac gagggaggtc ggatcaaccg
     1261 cgaccaagag ggaacggttc cccggccgag cccgctgacg atggcggttc ccccgccggc
     1321 gagccgcgaa cgctgtcgct ttctggtcga gaagctcgcc gaactaggtg ttgcccgttt
     1381 ggtttggctc tcgacgcgat tcggcgaggg ccatgtccca catcggacga aggcgatgtc
     1441 gtgggcggta tcggcgttgg aacagtcccg cggtgggtgg ttgatggaaa tcggggaggg
     1501 tctggtggcc tgggaagacc tgcatcggcc tcttgtggtg gcagtccccg gaggcggcag
     1561 cgaggtattt gaggtgatga cagttgctat cgggcccgaa ggtgggctcg accccgcgga
     1621 gatccccgcc gacgcggcca caatcgacct cggggtgacg attctgcgtg tggagactgc
     1681 tgcagtggtt gctgcaaccc gtttcagcag accctggcac gggggttcgt gaccttctag
     1741 tatctgcgca gggctcatct gggagagcaa tccttggtcg acaaattcag tgcgtcgctt
     1801 ggcgtccgtc ttagagcggc gcgtcgtcag cgaggctggt cattgggaga ggtggagtcg
     1861 ttcaccgacg gcgagttcaa ggcgtcggtc gtgggcgcgt acgagcgcgg ggaacgggcg
     1921 atctcggttc aacgactcac ccgtttggct gaggtctacg gagtaggccc gaccgagcta
     1981 tttcccggta ccaagccctc tgacggcccg gtgatcgaca tcgatgcggt cgctgccggc
     2041 cgggatcacg acatggttga tcgctatctg gcggctatcc agttcatgcg gcgcgaaggc
     2101 acaccaggcg aagtccgcga atccgacaag gcagtcattt cctccctgct ggagacttcg
     2161 atcgacgccg tgtccgagac cgactgaagt aatagcccaa atcgcctggg cggatagaat
     2221 taccgttggc gtcacaacta ggcgtcgttt tccgtgtctc ctgaacacat cgaatcgcat
     2281 ttacgcgtcc ccagccatca cttgttgttg gacctactag gggactctga tcgacacctc
     2341 cgccaggtcg aagaccactt cccggaggtg cg
//
LOCUS       DBNHXU010000196         2296 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3833380, whole genome shotgun sequence.
ACCESSION   DBNHXU010000196 DBNHXU010000000
VERSION     DBNHXU010000196.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2296)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2296
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3833380"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(502..909)
                     /locus_tag="AC161X_04915"
     CDS             complement(502..909)
                     /locus_tag="AC161X_04915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988923.1"
                     /translation="MVVVAPGLVVVVVVVPGLGEFRCLGGLRVWGFEAAVVVVSPGIV
                     VVGMVDTVVVLSPGGIVCPAAIGCPSVGWHSRDEKRLMSPSTLIADCNTYTRFPGPRG
                     SRRINQAWSGLRPITVIASPSKTSPNWGPPGQA"
     gene            complement(919..1128)
                     /locus_tag="AC161X_04920"
     CDS             complement(919..1128)
                     /locus_tag="AC161X_04920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988924.1"
                     /translation="MVDVSGTVVVVVGSSVVVVVSSVVVVVVGGSVVVVVDGAVVEVD
                     VEDPEVDVVVPAEVVVVTPGLVVVV"
     gene            complement(1154..>2296)
                     /locus_tag="AC161X_04925"
     CDS             complement(1154..>2296)
                     /locus_tag="AC161X_04925"
                     /inference="COORDINATES: protein motif:HMM:NF013109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR4988925.1"
                     /translation="MTTLVLGVSALCLILGLRFSASRFPGALVLVAGGILLTRLLDLQ
                     VALVGEVPSGLPSFVAPNLAYLADHLSVITPAAIGILLIGFSQSAGDAREFASRHKYR
                     VDIDRESIAQGVANVGSGLVQGIPVATSLSASSLNDQSGAKTQMSSLVTGGIVVLTML
                     FLAPLFSDLPKAVLAAVIIDAVLFGMIDLPGMGRLYRVQRFDFWISIAALLGVLTFGV
                     LAGVIIGMVLSIIWLIRSSTHPNVLELGNQPGTEQYRGLETYPDDTTFPGLLIIRFDG
                     ALFFATADTLHDRLRDLLRDRQPPIRTVVIDCESVNFVDSQGAEKLRELHELAGYNGI
                     ELWLTRLKSEVIETLARDGLVELFGEDRILTDTHGAVAAHLRNQEV"
BASE COUNT          508 a          712 c          708 g          368 t
ORIGIN      
        1 ggtggcggcg gcgatgatgg ccttcccgac gacccagcag agtggctggc cagcctcggg
       61 ggctcgattc gctcggatga cgaagaggaa taagcacttc cgaacgacag aacctctgaa
      121 aagggccacc gcgaggcggc ccttttcaca ttgacggcaa agtggccatt cgacatcgat
      181 acaaaccgcg ctttgccagt gccctagatg tgccgaagaa ttaccgatga cttctaatca
      241 ggacccgtca ggccccaaga aggggagacg gctcgcgccg gacgaactgg cgcgggacat
      301 cgccaatcgt ctgaaagtgt caggcggctc acacgtcgca gaccgacggt tcctgcctcg
      361 tttcgacctg ctcaatgcag gtcgcctcat catgggactt ggtgctctgc tgctgggcgc
      421 actcttgatc gcagggtttt cctggttgag cggcctgaac ggatatgaag cgccgggaag
      481 cgtcttcgag agctcgccgg tctatgcctg ccctggcggg ccccagttgg gggaggtttt
      541 cgaaggtgag gcgatcaccg tgatcgggcg gagccctgac caggcctggt tgatccttcg
      601 cgatccccgg ggccctggga atcgggtgta cgtgttgcag tccgcgatca aggtggatgg
      661 cgacatgagt ctcttctcgt cacgtgagtg ccagccgaca gatggacagc cgattgcggc
      721 gggacaaacg attccccccg gggagagcac caccacggtg tcgaccatcc cgaccaccac
      781 gattcctggc gagaccacga ccaccgccgc ttcaaatccc cagacgcgga ggccgccgag
      841 gcatcgaaac tcgccgagcc cgggcaccac cacgaccacg actaccagtc ccggggctac
      901 gacgaccacc cgccccggtc agaccacgac caccaagcct ggggtaacca cgaccacttc
      961 cgccgggacc accacatcga cctccgggtc ttcgacgtcg acctcgacca cggcgccgtc
     1021 gacgactacc acgacggatc ctcccaccac cacaaccaca accgaagaca cgacgactac
     1081 caccgaagag ccaaccacaa ccaccacggt gcccgagacg tcgaccactc tctgacaact
     1141 tcagcgaagg gtgctaaacc tcctgattcc ggagatgggc ggcaactgct ccgtgggtgt
     1201 cggtgaggat ccgatcctcg ccaaagagct cgaccagccc gtccctggcc agggtttcga
     1261 tgacctccga cttgagtcgg gtcaaccaga gctcgatccc gttgtaaccg gccagctcgt
     1321 ggagctcccg aagcttttcc gcgccctgag agtcgacaaa attcacgctc tcacaatcga
     1381 tgacgactgt ccggatcggt ggctgtcggt cgcgaagtag gtcgcgcaga cggtcgtgaa
     1441 gggtgtcggc tgtggcgaag aagagcgctc catcgaagcg gatgatcagc aggccgggaa
     1501 aggtggtgtc gtcaggatat gtctcgaggc cgcggtactg ctcggtaccg ggttggtttc
     1561 ctagttcgag gacgttcgga tgtgtcgatg accgaatcag ccagatgatc gagaggacca
     1621 tgccgatgat cacgcctgca agcacgccaa aggtcaggac tcctaacaag gccgcgatgg
     1681 agatccagaa gtcgaagcgt tgcacccggt agaggcggcc catgcccggc aggtcgatca
     1741 tcccaaacag aacggcatcg atgatgacag cggcaagcac ggccttggga agatcagaga
     1801 acaggggagc gagaaagagc atggtcaaca caacgatccc ccctgtcacc aacgaggaca
     1861 tctgcgtctt ggcccccgat tggtcgttga gcgagctggc ggagaggctc gtagctacgg
     1921 ggatgccttg caccaggcca gagccgacgt tggctacgcc ctgagcgatc gactcccggt
     1981 cgatgtccac ccgatatttg tgccgtgagg cgaattccct ggcatcgcca gccgactggg
     2041 agaagccgat cagcaagatg ccgatggccg cgggggtgat aaccgagaga tggtccgcca
     2101 ggtaagcgag gtttggcgcc acgaacgatg gaaggccact cggcacctcg ccaacaaggg
     2161 ccacctgaag atcgagcaat cgagtcagaa gaatcccgcc ggcgacgagt acgagggcgc
     2221 ccgggaatcg acttgcggag aaacgcagac cgaggatcaa gcaaagggct gaaacgccca
     2281 agacgagagt cgtcat
//
LOCUS       DBNHXU010000197        13457 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2840888, whole genome shotgun sequence.
ACCESSION   DBNHXU010000197 DBNHXU010000000
VERSION     DBNHXU010000197.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 13457)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13457)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13457
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2840888"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..883
                     /locus_tag="AC161X_04930"
     CDS             173..883
                     /locus_tag="AC161X_04930"
                     /EC_number="3.4.21.-"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015156201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HIR4988926.1"
                     /translation="MLPIRDINPTRITPVVTLALIAINIAVFFLFQMRGTALEQQELV
                     YERAAIACEVTTGAPLGIDEILDEVCLPGAQAPVFPNKNVFLALFTSMFLHGSVAHVL
                     FNMWFLWIFGNNVEEAFGHVGYLFFYLIGGLAATAVYVALNPDSTVPLVGASGAIAVA
                     LGAYAVLFPGRRVLSLVGWFLIPVPAAVFLAIWFALQFVAGQTGVAWEAHAGGFVFGA
                     GVTLLMRSRLLRRVAIAS"
     gene            989..1780
                     /locus_tag="AC161X_04935"
     CDS             989..1780
                     /locus_tag="AC161X_04935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4988927.1"
                     /translation="MVTLRGRLQVDTGPRPGLIAAASLLGLVVLGAALSSLFDAALLA
                     QIVATLVVPATVMVLFWRAVRARLKERDDEIGTMLDFERVSYRSLAEFLGDVSHELMV
                     HPERCSLRAESLAVAVDYQMVGPRLQVAVPDIVVVSDPNLLRQMLHILVGNALRHGGD
                     RVAIWAASDGDWASLTVSDDGPGIPPEMNDRILERLVDLGRPTPGIGDSGTGLSIARA
                     LSDLMGGQITYKRDASWSHFSFRFPVNSAGARPEPARARLGAGVV"
     gene            1784..2674
                     /locus_tag="AC161X_04940"
     CDS             1784..2674
                     /locus_tag="AC161X_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988928.1"
                     /translation="MHWRAAVQAVMIAVLWLGVLAPAASADDTETFFLGPDGVPVGSL
                     IGTPHGGQMPNYDRGRDLEPGLFLEKSDRGLLESEETRYQHWQAEMTGRHLAGYPTLV
                     VWSASAAFETGLTGTFSVYLLDCAESALECTELAGQEVVVPPADSGGWTETTVSLPQI
                     DHVFAEGRHLGIRIVVSDKSGTDMMFAYGFPKYRSRLIVSAEPPAITTEASVALPPIT
                     LAPLDRLGLVKPLSFLGFQEGPADINSLTPWLATLTGSTVILVVLGVVLVFTLSPHGR
                     RERSRPGTRPARNGRTVISS"
     gene            2715..3056
                     /locus_tag="AC161X_04945"
     CDS             2715..3056
                     /locus_tag="AC161X_04945"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR4988929.1"
                     /translation="MTSKSELERVASSLRQRRLGLSAELDRLVEPPESGASVGFGKRV
                     GDGTTEAVERISSTATARSIFASIEAIDSALASIEAGTYGVCENCGGEIPPARLEALP
                     ATSVCVDCARL"
     gene            3119..3391
                     /locus_tag="AC161X_04950"
     CDS             3119..3391
                     /locus_tag="AC161X_04950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988930.1"
                     /translation="MFQRGELQQIGKRFVVGGLLVTAITLLVIVADEVSSGGNAAIRA
                     TCVLFLWVGGVTITLGMILRIVGSGHAGTRPKPVPPRRARRSTNSR"
     gene            3468..4508
                     /locus_tag="AC161X_04955"
     CDS             3468..4508
                     /locus_tag="AC161X_04955"
                     /inference="COORDINATES: protein motif:HMM:NF033206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ScyD/ScyE family protein"
                     /protein_id="HIR4988931.1"
                     /translation="MKPLSKRLVTVLVTLALLAIPVLAGAVSGLAGPVFGLSTARNGS
                     LLVADASVGIHVVKKNQVKSTIPLPGVTDVSAASKNLLWAVTGAGEGGPNADTGQALY
                     RVWKGVPTQVINLFEFETDKNPDGNDPADSNPYDVQALSSQAALVVDAGGNDLLKVNK
                     NGRVRVLAVFPDEEVSTANFKALAGCPESAIPECDLPDTMMAQAVPTSVTIGPDGYYY
                     VGELKGFPGPTGESNIWRISPNASWAQCPSRKCVKVFDGGFTSIIDLAFHKGRLYVAE
                     LDESSWAAVEIFQDGVGGTINSCNVKRHSCRVVAEDIPQLTAITFDSHGKLWATQNSL
                     VPGGAEVVRIHQ"
     gene            4673..5413
                     /locus_tag="AC161X_04960"
     CDS             4673..5413
                     /locus_tag="AC161X_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988932.1"
                     /translation="MATTSATGSVRELPANPNITHLKGQAKDLQRAVRAGDRAAISRV
                     QASHPAPETRAGDGFGLRDAQLTLAREYGFESWNDLNREVGERMIEERDLHRWFGVQL
                     NNGMWDSIDDPSIGPDLPRERKEELLYSAYASAYHWRKVGNEANQARGEHLISRMAVK
                     IGENETARRHAERCLELVTTHAGVMEEWDEPFAHEALARALAAQGEALSAQSHRDLAI
                     EQTAALGDPEDREILEAELTREPWFGLI"
     gene            complement(5425..6765)
                     /locus_tag="AC161X_04965"
     CDS             complement(5425..6765)
                     /locus_tag="AC161X_04965"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HIR4988933.1"
                     /translation="MPEPIVIVVVLVALIAVGFLAYRFGRQGSPGEDETKRLEEYDFY
                     PFTVNDSRQVEFSRDHFNQAVGHFLNKRNSLAARELIVIGEQNFVRDAFATAEIDAYR
                     RLYDRYDGDSVISDNESYLENYKRMVSLIGRSFPNTGIEILLHNLVNPARSIVTLENG
                     EVTGRKLEMGTTNLVLDLKTRQSQQQDKLNYELNIGARQFKCTTIPIYRRDFGLVGAV
                     CINIDSRFIRETVKASPERLDAFINNLLKTEMQLEENILSPDEYQAALRGKRHYLDEA
                     IRASSDPGRRNTLRAIMFSDVVGYTPMMAGDEAAAIRTVETVRRIHRECITAQQGRLL
                     EEQGDGVLSMFGSVSAAVAAAREIQKEAQDDGSFQLRIGIHLGEVTESGGGIFGDGVN
                     IASRIHSLASPGTIVVSETVYDNVKNKGGVTAVDLGARELKGIQEPIHVYSIEV"
     gene            6912..7505
                     /locus_tag="AC161X_04970"
     CDS             6912..7505
                     /locus_tag="AC161X_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988934.1"
                     /translation="MSPSRLLIVGLALVVACGEGAATSTTLVSTTTTTVPSTTTTLQL
                     TTTAAPSTTVATGVSTTKVLDDSAEGSGCTPGEGPLPDGDWYGLVTSRGNDALDFDLA
                     CWFTGDAAVQASAEDGEESPPPNDYYVRNSNPDTREVVVGENIEVAFFPDGDPTNVTE
                     IDYDDWADMVATRGFELGVWLKVEDGVISEISEMWVP"
     gene            complement(7528..8028)
                     /locus_tag="AC161X_04975"
     CDS             complement(7528..8028)
                     /locus_tag="AC161X_04975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988935.1"
                     /translation="MADFDPVAALKVLVEHRVDFLVIGGVAARLRGAPFLTQDIDVTP
                     STDRANLERLALALKDLKARLRTTTEPDGFPFPFDSALLESADIWTLTTTHGDLDLVM
                     SPEGTGGYPDLIRDAEELRVSIDPELWVTVASLADVIRSKEAAGREKDRATLPLLRRT
                     LEESDG"
     gene            complement(8021..8335)
                     /locus_tag="AC161X_04980"
     CDS             complement(8021..8335)
                     /locus_tag="AC161X_04980"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4988936.1"
                     /translation="MTGGQLIREARKRGGLTQHQLALRLESHQSVVARWETGRSQPDF
                     DTVRKAVRAAGFDLGTTLSPTDDHDLGLIRRELKLLPHERLSRMVDAVRKFDAMSSAA
                     RG"
     gene            complement(8388..8798)
                     /locus_tag="AC161X_04985"
     CDS             complement(8388..8798)
                     /locus_tag="AC161X_04985"
                     /inference="COORDINATES: protein motif:HMM:NF020157.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1761 domain-containing protein"
                     /protein_id="HIR4988937.1"
                     /translation="MLFDYFADLNWLAVLVAALAYFVLGAIWYSNMLFGKAYRAALGI
                     TQQGTPNPSLLVINFVGWFITATALGLVGAAAGASSVLDGIVLGFVAGVGFVVVHQVV
                     AGTFDPGRRALMSVTVPYTVPGFVIMGVILAVWQ"
     gene            complement(8856..9194)
                     /locus_tag="AC161X_04990"
     CDS             complement(8856..9194)
                     /locus_tag="AC161X_04990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988938.1"
                     /translation="MERDEAAIDIDKPDHLLQKGPALGHLAALSYPGRSAFKPGSSDL
                     LDLLTSIVRGSWERNCMPDWVGTLVFVVAVGLSGAKISEDFGNRGLRGYAVLLITLLV
                     YASAVAVAFA"
     gene            9211..9624
                     /locus_tag="AC161X_04995"
     CDS             9211..9624
                     /locus_tag="AC161X_04995"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008933 - peptidoglycan lytic
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0000270 - peptidoglycan metabolic process
                     [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HIR4988939.1"
                     /translation="MVIALVSTLTPLHPAQAAENDCEVVTGTRLIDCGMDVRGEIGVD
                     AEWWRPMVALYFEPEDVERAMCLMGKESGGQPEARNSSTGAAGLMQVMPFWADRFGYS
                     YSELFDPGINLWVARQIRDEQGWRAWSPYLRGSCH"
     gene            complement(9629..9808)
                     /locus_tag="AC161X_05000"
     CDS             complement(9629..9808)
                     /locus_tag="AC161X_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988940.1"
                     /translation="MPRLFGIPCEHEQVMVVRSAGLERIVCEKCGHVSFSFDESELMA
                     ESELMVSAGTVADTD"
     gene            9934..10764
                     /locus_tag="AC161X_05005"
     CDS             9934..10764
                     /locus_tag="AC161X_05005"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HIR4988941.1"
                     /translation="MSLFALILALVAACLHAGWNLLVKASDDQLVAAWTVVAGAALLA
                     IPVLAYTGLPDSSVWDLIVISVIVQVVYVLMLALAYRLGDLSFVYPMARGTSPVLISL
                     AGVALLGDEISTVGWLGVLTVTAALTLLAWRRADRSGLWAALATGLMIATYTSVDAAA
                     VREEGAAIAVIAAEFILLAVTLGVVVLAVRGPHAMLGMVRDDWRKAAIGSVASGTAYL
                     LVMVAALSAPVGLVSGVRELSVLIAVIASRLLLHEEVTKSQLWTVAAAAGGIVLIALA
                     "
     gene            complement(10768..11499)
                     /locus_tag="AC161X_05010"
     CDS             complement(10768..11499)
                     /locus_tag="AC161X_05010"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HIR4988942.1"
                     /translation="MTRVEAGRAIFEVDAIAFDKDGTLVDLDAVWGPAGRAWVATAAA
                     GDDELEAILADRLGLDLVTGRLVPGGMIAVGSVNQIHEATLAILTEQGVEKEESQSLA
                     KAARSTAVMAADAQGQVPLGDVAGTLMRLQEAGLRLAVVSSDNRAAVDAAILALGIRS
                     LIDAVVAGDDGFDAKPAPDALLEAARRLGVEPNQMLYVGDSWVDAGAGRAAGIAGTVL
                     VGEPTAEARGLASVVVPGVDSLRVV"
     gene            complement(11507..12562)
                     /locus_tag="AC161X_05015"
     CDS             complement(11507..12562)
                     /locus_tag="AC161X_05015"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012119764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4988943.1"
                     /translation="MSGGFLSLEDVRKEFNGVVAVDTFNLEVEPGEFVSFLGPSGCGK
                     TTTLRMVAGFEQPSSGTIRLAGEDVTYAKPNERNIGMVFQSYALFPNMTVSENVAFGL
                     RIRKEDSTARKARVDELLDLIHLPDKGDSYPNELSGGQQQRVALARALAIRPQLLLLD
                     EPLSALDAKIRDELRTEIRRIQRQLNITTIYVTHDQEEALALSDRIVVMSKGHMEQVG
                     APFEIYNNPSTGFVASFVGQLNRLPATVIDRDQGRLRVGTDEVRTTSGVESDSGTEVS
                     LMLRPEELTLDASADNVLDGTVESVTFLGSIVRIQVAVSGGNLTADLFNERLLTLPVA
                     GDPTQISFPAHACWVMV"
     gene            complement(12559..13386)
                     /locus_tag="AC161X_05020"
     CDS             complement(12559..13386)
                     /locus_tag="AC161X_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988944.1"
                     /translation="MATTPLPTAAEAPSVDAVPAKRNRFWAWLIFTLVALYFLVPLGA
                     TFWWSLRAEKGVLGFEAYRRLFEDSRFWPSLTESVFNAVAAIFLSLLIIVPTAYWVTL
                     KLPKLRPVIEFITLLPFVIPAVVLVFGLIRLYGRPPLLLTATYGSSRVLLICAYAALS
                     FPYMFRSVDNGLRAIDVRSLTEAAQSLGASWPTILRKVIFPNLRVALLSGALLTFAIV
                     IGELTIALFMAQHTFGPYMAELTRSKVYEPSAMAIVAFAITWAAMGMIALITREKRR"
BASE COUNT         2400 a         4137 c         4337 g         2583 t
ORIGIN      
        1 tcgctccacc cgagccgtcc ggcgacgccg agaaggagcg tcaccaggta gaggttgatt
       61 ccggacgccc atcccgatcc ggcgatgatt ccgaaggctt ccacgggaag aaggtagcgc
      121 tggatccgag caccggtccg taccatctcc ttgtctagag acgtaaggcg cgatgctccc
      181 gatacgtgat atcaacccga cccggatcac ccctgtggtg accctggcgc tgattgcgat
      241 caacatcgcg gtgttcttcc tgttccagat gcggggcact gctctggagc aacaggagtt
      301 ggtctacgag cgggcggcta tcgcatgtga ggtcaccacc ggcgcacctc tcggaatcga
      361 cgaaattctc gacgaggttt gcctgccggg cgctcaggcc ccggtgtttc cgaacaagaa
      421 cgtcttcctc gccttgttca cgtcgatgtt cctccacggc agcgtggctc atgtcctgtt
      481 caacatgtgg tttctttgga tcttcggaaa caatgtcgag gaagccttcg ggcatgtggg
      541 ctatctcttc ttctatttga tcggcggcct cgccgccact gccgtctatg tggcgctcaa
      601 cccggattcg acggttcccc tggtgggcgc ttcgggggcg attgcggtcg ctctcggcgc
      661 ctacgcagtc ctgtttccgg gtcgccgggt gctgtcactc gttgggtggt tcctgatacc
      721 ggtcccggcc gcggtgttcc tggcgatctg gtttgccctc caattcgtcg ccggccagac
      781 gggtgtcgcc tgggaggccc acgcaggcgg gttcgtcttc ggcgccgggg tgaccctgct
      841 catgcgctcg cgcctgctcc ggagagtggc gatcgcctcg taattgagac ggggaggtcc
      901 gtatatgacc cctcctggtc ccaattacgg agtcgcgaac ttcggttggt ctggctatag
      961 ccaggcctga agcgaccgat aaagaggggt ggtcacccta cgtgggaggt tgcaggtcga
     1021 caccggcccc cggccggggt tgattgctgc tgcctcgctt ctcggtttgg tggtgctcgg
     1081 cgcagctctt tcgagtctgt tcgacgccgc tctccttgct cagatcgtgg ccactctggt
     1141 cgttccggca accgtcatgg tcctgttctg gcgggccgtg cgcgcccggt tgaaggaacg
     1201 cgatgacgag atcgggacga tgctggactt tgaacgggtt tcgtatcggt ccctcgccga
     1261 gtttctcggg gatgtctccc atgagttgat ggttcacccc gagcgttgca gtctccgcgc
     1321 cgagtccctg gccgttgcgg tggactacca gatggtgggc ccccgccttc aggttgccgt
     1381 gcctgacatc gtcgtggtct cagatcccaa cttgctcaga caaatgctcc acatcctcgt
     1441 gggcaacgcc cttcgtcacg gcggagatcg ggtggcgatc tgggctgcaa gcgatgggga
     1501 ttgggccagc ctcacggtct ctgacgatgg accagggatc ccacctgaga tgaacgaccg
     1561 aattctggag cgactggtcg atctgggccg gccgactcca gggattggcg actcaggaac
     1621 cggcctttcg attgctcgtg ccttgagtga cctgatgggt ggccagatca cctacaagcg
     1681 cgacgccagt tggagtcatt tctcgttccg cttcccggtc aattctgctg gggcccgtcc
     1741 tgaacccgcc cgggcccgcc tcggtgcggg ggtggtttga cggatgcatt ggagggccgc
     1801 agtgcaggcc gtgatgatcg cggtgctgtg gctgggggtc ctggcacccg ccgcatcggc
     1861 cgatgacacc gagaccttct tcctcgggcc agacggtgtg cctgttggct ccctgatcgg
     1921 aaccccccac ggtgggcaaa tgcccaacta tgaccggggt cgggatctag aacccgggct
     1981 cttcctcgag aagtccgatc gcggtctcct cgagagcgag gagactcggt atcagcattg
     2041 gcaggcggag atgacgggtc gccatctggc cggctacccg actctcgtcg tgtggagcgc
     2101 ctcggcggcg ttcgagactg gcctcaccgg caccttctct gtctatctgc tcgactgcgc
     2161 tgagtccgcc ctcgaatgca ccgagttggc cggccaggag gtagtcgtcc cgcccgccga
     2221 ttcgggaggg tggaccgaga ccacggtgtc ccttcctcag atcgaccatg tgttcgccga
     2281 gggccgacat ctcggaatca gaatcgtcgt ctccgacaag tcgggcaccg acatgatgtt
     2341 cgcctacggg tttcccaagt atcgaagccg tctcatcgtc tccgccgagc ctccggcgat
     2401 aacgactgag gccagcgtgg cgttgccacc gatcaccctg gctccccttg accggctcgg
     2461 gctggtgaag ccattgtcgt tcctgggctt tcaggaagga ccggccgaca tcaactccct
     2521 gaccccctgg ctcgccactc tcaccgggtc gaccgtgatc ctggtcgtcc ttggggtcgt
     2581 cctggtgttc accctgagcc ctcacgggcg gcgagagagg tcgcgaccag gcacacgccc
     2641 cgcccgcaat ggaagaaccg tcatctcgag ttgaggcatc ggtccccacg atccaatcaa
     2701 ctaccgtccg cttgttgact tcgaagtcgg aactggagcg agtcgcttcg tctcttcgcc
     2761 agcggcggct gggactgagt gcggagttgg atcggctcgt cgagcccccc gagagtgggg
     2821 catcggttgg cttcggcaag agggtgggag acggcaccac cgaggcagtc gagaggatct
     2881 ccagcaccgc gaccgcccga tccattttcg cctcgatcga ggccatcgat tcagcgctcg
     2941 ccagcatcga ggccggcacc tacggagtgt gcgagaactg cggtggcgag atcccgccgg
     3001 cgcgtctcga ggcgcttccg gcgacgtcag tgtgcgtgga ttgcgcccgc ctctgatcgt
     3061 gtgatctggg ggaggatccc catggacgct ccgtgcccgg ctctggcaag attgggacat
     3121 gttccagcgg ggcgagctgc aacaaatcgg caagagattc gtggtcggtg gactgctggt
     3181 cactgcaatc acccttctcg tcatcgtcgc tgatgaggtc tcgagtggcg gcaatgccgc
     3241 gatcagagcg acttgcgtcc tcttcctctg ggtgggaggg gtgacgatca cgcttgggat
     3301 gatcttgcgc attgttgggt cgggacacgc cggcaccaga ccaaagcctg tgccgccccg
     3361 acgagcgagg cgatctacca actcccggtg acctcagcgt ctaggcagac cgttaaaggg
     3421 gtaggtttcc cggctatcgg aagtcgcccc ccttcgaaag gtgctgcatg aaaccgctct
     3481 ccaagcgact cgtcaccgtc ctagtgacgc tcgcgttgct ggccatacct gttctcgccg
     3541 gagccgtcag cggtcttgcc gggcctgtgt tcggcctgag cactgcgcgc aacggcagcc
     3601 tgctggttgc cgatgccagt gtgggcatcc atgtcgtcaa gaagaatcag gtgaagtcca
     3661 ccattccgct gccgggtgtg accgacgtca gcgccgcgag caagaacctc ctctgggccg
     3721 tcaccggcgc cggggaaggc ggacccaacg ccgacaccgg acaggccctg taccgggtct
     3781 ggaagggggt ccccacccaa gtcatcaatc tgttcgagtt cgagaccgac aagaaccccg
     3841 acggaaacga ccccgccgat tccaacccct atgacgtgca ggctctgagc tcgcaggcgg
     3901 cgttggtggt cgatgccggt ggcaacgatc tgctcaaggt gaacaagaac gggagagtca
     3961 gggtcctggc ggtgttccca gacgaggaag tctccacagc gaatttcaag gcgctggcgg
     4021 gatgtcccga gtcggccatc cccgagtgtg acctgccgga caccatgatg gcgcaggcgg
     4081 tgcccaccag cgtgaccatc ggtcccgatg gctactacta cgtcggtgag ttgaagggct
     4141 ttcccgggcc gacaggtgag tccaacatct ggaggatcag cccgaatgcc tcatgggcgc
     4201 aatgcccgag ccggaaatgt gtcaaggtct ttgatggtgg cttcacctcg atcatcgacc
     4261 tcgcgttcca caagggccgt ctctacgtcg ccgagctcga tgagtcgagt tgggctgctg
     4321 tggagatctt ccaggacggc gttggcggca ccatcaactc ctgcaacgtg aagcgtcaca
     4381 gttgtcgggt tgtggctgag gacatcccgc agttgacagc gatcactttc gatagccacg
     4441 gcaagctgtg ggcaacccag aactccctgg tcccgggcgg ggctgaggtg gtccgaatcc
     4501 accagtaaat ccgactgggc taaaccggcg cgcattcgct aaaatcgcca gcagtgcgcg
     4561 tcggggcgcc tctcgtccgc ggcaagagcc gagctcaccc gaccaacgat ccgatgaagc
     4621 gatctttctt ccgcccggat cagcggacgc gggtgtggga aggaaggtca tcatggcaac
     4681 cacgtcggct accgggtcgg tccgggaact ccccgccaac ccaaacatca cccacctcaa
     4741 gggacaggcc aaggacctgc agcgcgcggt gcgggccggg gatcgggctg cgatatcccg
     4801 agtgcaggca tcgcacccag cacccgagac tagggccggg gatggcttcg gccttcgaga
     4861 cgcccaactg actctggccc gcgaatacgg gttcgagagc tggaacgacc tcaatcgtga
     4921 ggttggcgag cgcatgatcg aagagcgcga cctgcatcgc tggtttgggg ttcagctcaa
     4981 caacgggatg tgggacagca tcgacgatcc ctccatcggc cccgatctac cgcgggagag
     5041 gaaggaagag ctcctctatt cggcctacgc ctccgcctat cactggcgga aggtggggaa
     5101 cgaggcgaac caggcccggg gtgagcatct catttcgagg atggcagtga agatcgggga
     5161 aaacgagacg gcccggcgcc atgccgaacg ttgtctcgag ctggtgacga ctcatgccgg
     5221 agtgatggag gaatgggacg agccgtttgc ccatgaggct ctggcccgag cgctggctgc
     5281 tcagggtgaa gcgctttcgg cgcagtcgca tcgtgatctc gccatcgagc agaccgcggc
     5341 gctcggcgat cccgaggatc gcgagatcct cgaggccgaa ctgacgcggg agccctggtt
     5401 cggtctgatc tgacctgggg tcggtcaaac ctcgatggag tagacgtgga tcggctcttg
     5461 gatgcctttc agctctcggg caccgaggtc gacagcggtg accccgccct tgttcttgac
     5521 gttgtcgtag accgtctcgg aaacgacgat cgtccccggg ctggcgagac tgtgaattcg
     5581 agaggcgatg ttgactccgt ctccaaatat tccgccgcca ctttcggtga cttcaccgag
     5641 atgtatcccg atccgcagct ggaatgaccc atcgtcctgg gcctccttct ggatctctct
     5701 ggcggccgcc actgctgcgg agaccgaccc gaacatggac aacacgccgt caccctgctc
     5761 ttcgagcagt cgtccttgct gagcggtgat gcactcgcga tggatccgac ggacggtctc
     5821 caccgtccgg atggcagccg cctcgtcccc ggccatcatg ggcgtgtagc cgaccacgtc
     5881 ggagaacatg attgcccgaa gcgtgttgcg ccggccaggg tcactgctcg cccggatcgc
     5941 ctcgtcgagg tagtggcgtt tgcctcgcag cgcagcctgg tattcgtcgg gggagaggat
     6001 gttttcctcg agctgcatct cggtcttgag gaggttgttg atgaaggcgt ccaaccgctc
     6061 ggggctcgct ttgaccgtct cccggatgaa ccggctgtcg atgttgatgc agaccgcccc
     6121 gaccagaccg aagtcacggc gatagatggg aatggtggtg catttgaact ggcgggcacc
     6181 gatgttgagc tcgtagttga gcttgtcctg ctgctggctc tggcgggtct tgaggtccag
     6241 gacaaggttg gtcgttccca tctcgagctt tcgcccggtg acttcaccgt tttcgagggt
     6301 cacgatggag cgagccgggt tgaccagatt gtgaagcagg atttcgatac cggtgttggg
     6361 gaaggaccga ccgatcaggc tgaccattcg cttgtagttc tccaggtatg actcgttgtc
     6421 ggagatcacc gaatccccgt cgtagcggtc gtagagacgg cgataagcat cgatctcggc
     6481 tgtggcaaag gcatcccgaa cgaagttctg ctctccgatg acaatcagct cacgagcggc
     6541 gagtgagttt cgcttgttca ggaagtggcc gacggcctgg ttgaagtggt cacggctgaa
     6601 ctcgacctga cggctgtcgt tgacggtgaa cggatagaag tcgtattcct ccagccgctt
     6661 ggtttcgtct tcacctgggg agccttgccg tccgaatctg taggcgagaa agccgacagc
     6721 gatcagcgcc acaaggacca caacgatcac gatcggttcg ggcacccttc aaggctatcg
     6781 ctccggtgct gatgaattgg tgacgccatg cctgatcagt cgggcccggg gcgcgctttg
     6841 agatgcggtt acccatcgcc gaatagcggt tgtcgtaggc tgaactcgct caggatccga
     6901 ttggagccct tatgagtccc tcccgtcttc tgatcgtcgg tctggcgttg gtggtggcat
     6961 gtggcgaagg cgccgcgacc agcaccaccc tggtttcgac aaccacgacc accgtgcctt
     7021 cgacgacaac cacccttcag ctcacgacaa cggccgctcc ctcaaccacc gtcgccactg
     7081 gagtttccac cacgaaagtg ctggacgaca gcgctgaagg gtcaggttgc acgccgggtg
     7141 agggcccgct ccctgatggc gattggtatg ggctggtcac ctctcgcggt aacgacgcgc
     7201 ttgacttcga ccttgcctgc tggttcaccg gtgatgccgc agtacaggcg tcggccgaag
     7261 acggcgaaga gtccccgccg ccgaacgact attacgtgcg caactcgaat cccgacaccc
     7321 gcgaggtggt ggttggcgag aacatcgagg tggccttctt ccccgacggc gaccccacca
     7381 acgtgaccga aatcgactac gacgactggg ccgacatggt ggcgacacgc ggcttcgagc
     7441 tgggggtctg gctcaaagtg gaggatgggg tgatctcgga gatctccgag atgtgggtgc
     7501 catgagcccc gaagctcgga gaggccgcta gccgtcggac tcctccaggg tccgccggag
     7561 taacggcagg gttgcccggt ctttctcccg gcctgccgcc tccttgctcc ggatgacgtc
     7621 cgccaaggag gccacggtta cccagagttc gggatcaatt gagactcgaa gctcctctgc
     7681 atcccggatc aggtccggat agccgccagt cccctccggg gacatcacga gatcgaggtc
     7741 cccatgcgtt gtggtgaggg tccaaatatc ggccgattcg agaagggcgg agtcgaaggg
     7801 aaaagggaat ccgtccggct cggtggtcgt gcgaagtcgg gctttcagat ccttcaaggc
     7861 cagggccaga cgttcgaggt ttgcacgatc agtggatggg gtcacatcga tgtcctgggt
     7921 caggaagggt gctccccgca atcgggcggc caccccacct atcaccagga agtcgacccg
     7981 gtgctccacc agcaccttca atgccgccac cgggtcgaag tcagccacgg gccgcgctgc
     8041 tcatcgcgtc gaacttccgc accgcgtcga ccatgcggga caatcgctca tggggcaaga
     8101 gcttcagttc gcgtcgaatg agacccaggt cgtggtcatc agtgggggag agggtggtgc
     8161 cgaggtcaaa accggcagcc cgaaccgcct tgcggaccgt gtcgaagtcg ggttgggagc
     8221 gtccggtttc ccaccgtgcc accaccgatt ggtgactctc gagtcgcagg gccaattggt
     8281 gctgggtcag accaccacgt ttgcgcgcct ctctgatcag ttggccgccg gtcatctgcc
     8341 cattatagca aatacgctat ggaagagata gcgtatttgc gataccttta ctgccagacg
     8401 gccaggatca cacccatgat cacgaagcca ggaacggtgt aggggacggt cacgctcatc
     8461 aatgcccgcc ggcccgggtc aaaggtgccg gcaaccacct ggtggacgac cacgaagccg
     8521 actcccgcaa cgaagccgag aacgatcccg tcgagtaccg acgaggcccc tgcggccgcc
     8581 cctaccaacc caagggcggt ggccgtgatg aaccagccga cgaaattgat caccaacagc
     8641 gagggattcg gcgtgccctg ctgggtgatg cccaaggcgg cccggtaggc cttgccgaac
     8701 aacatgttgg aataccagat ggcgccgagg acgaagtagg cgagggcggc gacgaggaca
     8761 gcgagccagt tcaggtcggc gaaatagtcg aaaagcaatt gagcctcctc aggtcaaccg
     8821 aaggaagcta cgccactgga tcgacagaag ttggctcagg cgaaggcgac agccaccgcc
     8881 gacgcgtaga ccagaagcgt tatcaagagc acggcgtagc cgcgcagacc gcggttgccg
     8941 aagtcctctg agatcttggc tccagacagg ccaaccgcga cgacaaacac aagggtccca
     9001 acccaatccg gcatgcagtt ccgctcccaa ctccccctca cgatggaagt tagcaaatcg
     9061 agaaggtcgc tgctccctgg tttgaaggcc gagcggccgg ggtaggagag cgccgccagg
     9121 tggcccaggg cgggcccctt ttgtaggagg tggtcgggtt tgtcgatgtc tatcgcggct
     9181 tcgtcgcgtt ccaagcaggt tctgatcgtg atggtgatcg ctctcgtgag cactctgacc
     9241 ccgttgcatc cggcgcaggc agccgaaaac gactgtgaag tggtcaccgg cacccgtctc
     9301 atcgattgtg gaatggacgt tcgtggggag attggagtcg atgccgaatg gtggcgtccg
     9361 atggtggctc tctatttcga gcccgaggac gtggagcgtg cgatgtgtct gatgggcaag
     9421 gagagcggag gccaacctga ggcccgcaac tcgagcaccg gtgccgcggg tcttatgcag
     9481 gtgatgccgt tctgggccga caggtttgga tacagctaca gcgagttgtt cgacccgggg
     9541 atcaacctat gggtggccag acaaatccgc gacgaacagg ggtggcgggc ctggagccct
     9601 tacctgaggg gctcctgcca ctagccgatc agtcggtgtc tgcgaccgtc ccggcgctga
     9661 ccatcaactc ggattcagcc atcaattcgc tctcgtcgaa gctgaagctg acatgaccgc
     9721 atttctcaca cacgatccgt tccagaccgg cggaacgaac caccatgacc tgctcgtgtt
     9781 cacaagggat tccgaataac ctgggcaagt tcactctcac tctgagtgcc tccatctcgc
     9841 tcttcgtgac atcttgatcg aagagatgcg gaaaccggcg gcaatccggt cgcagtgctg
     9901 aaatctgctg gtgtctgagt ccgccggaac gcgatgagcc tgttcgcgct gatcctcgct
     9961 ctggtcgccg cctgtctgca cgccggatgg aatctgttgg tgaaggccag cgacgaccaa
    10021 ttggtggcgg cctggactgt ggttgccggg gcggcgctcc tggcgattcc ggttctcgcc
    10081 tacaccgggc ttcccgactc ttcggtatgg gacctcatcg tgatctcggt gatcgtgcag
    10141 gtggtgtacg tcttgatgct ggccctggcg taccgcctcg gcgatctctc gttcgtttac
    10201 ccgatggcgc ggggcacgtc cccggttctg atctccctcg ccggggtggc cttgctcggc
    10261 gacgagatct cgactgtggg ctggctggga gtgctcaccg tgaccgcggc tctcaccctg
    10321 ctcgcctggc ggagggctga tcgctcgggg ttgtgggccg ccctcgctac cgggttgatg
    10381 atcgccactt acacctcggt ggacgccgcc gcggtgcgtg aggagggagc ggcgattgca
    10441 gtgatcgcag ccgaattcat actcctcgcg gtcacactgg gggtagtcgt tctggccgtc
    10501 cgcggccccc acgcaatgtt gggaatggtc agagatgact ggcgcaaggc cgcgatcggc
    10561 agcgtcgcct caggcactgc ctacctgctg gtgatggtcg ctgccctcag cgccccggtg
    10621 ggcctggtgt caggggtcag ggagctctca gtcctgatcg ctgtcatcgc gtcgcgcctc
    10681 ctgctgcatg aagaggtgac gaagtcgcag ttgtggacgg tggcggccgc ggccggtggc
    10741 atcgtcctca ttgccctcgc ctgaggctca caccacgcga agcgagtcga ctcccggcac
    10801 cacaacggac gccagcccac gcgcctcggc ggtcggctca ccgacgagga ccgtgcccgc
    10861 gattccggca gctctgcctg ctccggcatc gacccaggag tcaccgacgt aaagcatctg
    10921 gttcggctcc acccccaggc gcctggctgc ctcgaggaga gcgtcgggag cgggcttggc
    10981 atcgaaacca tcgtctcccg ccaccacggc gtcgatcagg gatcggatcc ccaacgccag
    11041 gatcgcggca tccaccgccg cacggttatc gctggagacg accgccaacc ggagcccggc
    11101 ctcctggagc ctcatgaggg ttccggccac atctccaagc gggacttggc cttgagcgtc
    11161 ggcggccatg accgctgtgg accgggcggc cttggccagg ctttgtgact cctctttctc
    11221 gacgccctgc tcggtcagta tcgcgagggt ggcttcatgg atctggttga cgctgccgac
    11281 agcgatcatc ccgcccggca ccaggcggcc cgtgaccaga tccaacccga gccggtcggc
    11341 gaggatcgcc tccagttcgt cgtctccggc ggccgcggtc gctacccagg ccctccccgc
    11401 cgggccccac accgcatcga ggtcgaccag agtcccgtcc ttatcgaacg cgatcgcatc
    11461 aacctcgaag atggcgcgtc cggcttcgac ccgtgtcacc tcgaggtcag accatcaccc
    11521 agcaggcgtg ggcggggaaa gagatctgag tggggtcgcc cgccaccggc agcgtcaaca
    11581 ggcgctcgtt gaacaggtcg gccgtgagat tgccaccgct cactgccacc tggatgcgga
    11641 cgatcgatcc gaggaaggtg acgctttcca ccgttccgtc gagaacgttg tcggccgacg
    11701 cgtccagggt cagttcttcg ggtcgcagca tcagagagac ttcggtaccc gaatccgact
    11761 cgacacccga cgtggtccgc acctcgtctg ttccaacccg caatctgccc tggtctcgat
    11821 cgatcaccgt ggccggtaga cggttgagtt ggccgacgaa agaagcgacg aaccctgtgg
    11881 atgggttgtt gtagatctcg aacggagcgc cgacctgttc catgtggcct ttgctcatca
    11941 ccacgatccg atcggagagg gcgagggcct cctcttgatc gtgggtgaca tagatcgttg
    12001 tgatgttgag ctggcgttgg atgcggcgaa tctcggtcct cagctcgtcg cggatcttgg
    12061 cgtcgagggc ggagagcggc tcgtccaata gaagaagttg ggggcggatc gccagggctc
    12121 gggccagggc aacccgctgt tgttgcccgc cggacagctc gttggggtag gagtcaccct
    12181 tgtcagggag gtgtatcaaa tcgaggagtt cgtcgactcg tgccttgcgg gccgtgctgt
    12241 cctccttgcg gattcggagc ccgaatgcga cgttctccga gacggtcatg ttggggaaca
    12301 gggcgtagga ctggaacacc atgccgatgt tgcgctcgtt gggctttgcg taggtgacgt
    12361 cctccccggc cagccggatg gtgcccgaac tgggctgctc gaagccggcc accattctca
    12421 gcgtggttgt cttcccgcag ccggaaggcc caaggaagct gacaaactcg cccggctcga
    12481 cttcgaggtt gaaggtgtcc actgcgacga cgccgttgaa ctccttgcgc acatcttcca
    12541 ggctgaggaa tcctccgctc atcgcctctt ctccctcgtg atgagggcga tcatccccat
    12601 ggccgcccag gtgatggcga aggccacgat cgccatcgcc gacggctcat agaccttgct
    12661 ccgggtgagc tctgccatgt aggggccgaa cgtgtgctgc gccatgaaga gggcgatggt
    12721 cagctcgccg atgacgatgg cgaaggtgag gagggccccg gagaggaggg ctacccggag
    12781 gttggggaag atcaccttgc gcaggatcgt gggccagctt gccccgaggc tctgggccgc
    12841 ctcggtgagg gagcgcacgt cgattgctcg caacccgttg tccacggagc gaaacatata
    12901 tgggaaggac agggcggcgt aggcgcagat gagtaggacc cgggagcttc cgtaggtggc
    12961 agtaagtagg agcggtggac gcccgtagag acggatcagg ccgaaaacca acaccacggc
    13021 tgggatcacg aacggaagca gagtgatgaa ctcgatgacc gggcgcagct tgggcagttt
    13081 gagggtgacc caatacgcgg tgggcacgat gatcagcagg ctgagaaaga tggcggcgac
    13141 cgcattgaac accgactcgg tcaatgacgg ccagaagcgc gaatcttcga acagccggcg
    13201 atatgcctcg aatccaagca cgcccttctc cgcccgcagc gaccaccaga aggtggcgcc
    13261 caaggggaca aggaagtaga gggcgaccag tgtgaagatc aaccacgccc agaaccggtt
    13321 gcgctttgcc ggaaccgcgt cgaccgatgg cgcctctgcc gcggttggaa gcggagtcgt
    13381 ggcaatcgtc accccaacca cctttcagag cgtcgctgca ggatcgagta gatcaggatg
    13441 ctcccggcca tgatgaa
//
LOCUS       DBNHXU010000198         2636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3833545, whole genome shotgun sequence.
ACCESSION   DBNHXU010000198 DBNHXU010000000
VERSION     DBNHXU010000198.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3833545"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            55..2475
                     /locus_tag="AC161X_05025"
     CDS             55..2475
                     /locus_tag="AC161X_05025"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HIR4988945.1"
                     /translation="MPEISVSGTIGGKEFVLSTGKLAGQADGAVVAQLGGTEMLVTAT
                     ASKSLREGVDFFPLTVDFEERMYAVGKIPGSFFRREGRPSEQAILTCRLIDRPLRPSF
                     ADGYRCETHVVITSLAVDGENPYDVVALNGASAALAISPIPFQGPIGGVRLGLKKGEW
                     IPFPTEADLAESVFELVVAGGRNDAGGIDIIMVEAGSTPNGLRLIADGDAPSDEATVA
                     RGLEEAKTYIGESIDLQNELVSKVTKKEVEWPVAVDYTTEVYDRVKAIAEPKLQSVVL
                     IAEKQERNAAQDAALESTMAELALDEDDSDGREQSKRAFKSVVKDLMRKRVVEDGIRL
                     DGRGPTDIRTLSAEVSVIRRTHGSGLFQRGETQVLNLTTLGMLKMEQMLDTLANEDSK
                     RYMHHYNFPPFSTGEAGFMRGPKRREIGHGALAEKAVLPVIPTEEEFPYALRLVSEVL
                     SSNGSTSMASVCASSLSLMDAGVPIAAPVGGIAMGLIHRDGKYVTLTDILGAEDALGD
                     MDFKVAGTRDVITALQLDTKLEGLPSEVLADALDQAKVARLIILDKMAEAIAEPRSEM
                     NEFAPRIESIEIPKDKIGEVIGPKGAVIRELEEVTGAAIEIEEADGKGIVRIASSDSS
                     ALAAAKEKVMQIAFPPEVELGTEYTGKVVNITKFGAFVNILPGRDGLLHISRLDGSKR
                     VERVEDYLTDGQELKVRVREIDRGKVSLELIEALEGATLPESEPPSSGGGDRGRGSGD
                     RDRGRGGSGDRGRGGSGDRDRGRDQNRDDRPAPEKAPVAIAQPTSASSERRRVAQPFD
                     EVFEDLQN"
BASE COUNT          506 a          822 c          842 g          466 t
ORIGIN      
        1 tctgagacat gactactgcc aacgggcgtg gctccattac acaaggagca cttagtgccc
       61 gaaatctccg tatcggggac catcggtggc aaagagttcg tcctgagcac cggcaaattg
      121 gccggacagg cggacggagc cgtggtcgcc caactcggcg gtaccgaaat gttggttacc
      181 gccacagcaa gcaagtcgct acgcgaaggc gtcgacttct tcccactcac cgtggacttc
      241 gaagagcgga tgtacgccgt gggcaagatc cccggctcgt tcttccgccg cgagggtcgt
      301 cccagcgagc aggccatcct gacctgccgt ctcatcgacc gcccgcttcg cccctcgttc
      361 gccgatggtt accgctgcga gacccacgtg gtcatcacca gcctcgccgt cgatggcgag
      421 aatccctatg acgtagtggc cctcaacggc gcctctgccg ccctcgccat cagcccgatc
      481 ccattccagg gaccgatcgg tggagttcgt ctgggactca agaagggcga atggatcccg
      541 ttcccgactg aggctgacct cgcagagtca gtcttcgagc tggtcgtcgc cggaggtcga
      601 aatgacgccg gtgggatcga catcatcatg gtcgaggccg gctcgacccc caacggtctg
      661 cgtttgatcg ccgatggcga tgccccttcc gacgaggcca cagtggcccg cggtttggag
      721 gaagccaaga cctacatcgg cgagtcgatt gaccttcaga acgagctggt ttccaaggtc
      781 accaagaagg aagtcgaatg gcctgtggcc gtcgactaca cgaccgaggt ctacgacagg
      841 gtgaaggcga tcgccgagcc caagttgcag tcggttgttc tcatcgccga gaagcaggag
      901 cgcaacgctg cccaggatgc agccctcgag tccacgatgg ccgagttggc tctcgacgag
      961 gacgactccg atggtcgcga gcagtcgaag cgggccttca agtcggtggt caaggacttg
     1021 atgaggaagc gtgtcgtcga ggacggaatc cgtctcgatg gccgcggccc gaccgacatc
     1081 cgcacactca gcgccgaagt cagtgtgatc aggcggaccc atggctccgg cctcttccag
     1141 agaggcgaga cccaggttct gaacctgacc acgcttggca tgctcaagat ggagcagatg
     1201 ctggacaccc tcgccaacga ggattccaag cgctacatgc accactacaa cttcccaccg
     1261 ttctcgacgg gtgaggctgg gttcatgcgt ggtcccaagc gccgtgagat cgggcatggg
     1321 gccctggccg aaaaggccgt gctcccggtg atcccgaccg aggaggagtt cccctacgcc
     1381 ttgaggctgg tgtccgaggt tctttcctcg aacggatcca cctcgatggc gtcggtgtgc
     1441 gcatcctcac tgtccctgat ggacgccggg gtcccgatcg cagcgcctgt cggcggaatc
     1501 gccatgggcc tgatccatcg ggatggcaag tacgtcaccc tcaccgacat cctcggtgcc
     1561 gaggatgcac ttggtgacat ggacttcaag gtcgccggca ccagggacgt gatcaccgcg
     1621 ctccagctcg acaccaagtt ggagggcctg ccctccgagg tgttggccga cgccctggat
     1681 caggccaagg tggcccgtct catcatcctc gacaagatgg ccgaggcgat tgccgagcca
     1741 cgttccgaga tgaacgagtt cgcgccgcgc atcgagtcga tcgaaatccc caaggacaag
     1801 atcggtgaag tcatcggtcc caagggtgcg gtcatccgtg agctcgaaga ggtcaccggc
     1861 gccgccatcg agatcgaaga ggctgatggc aaggggatcg tcagaatcgc ctcctccgac
     1921 agctccgcac tggcggctgc caaggagaag gtcatgcaga tcgcattccc gcccgaggtc
     1981 gagttgggga ccgagtacac cggcaaggtg gtcaacatca ccaagttcgg tgccttcgtc
     2041 aacatcctgc ccggacgtga cggtttgctg cacatctcgc gtctcgacgg ctccaagaga
     2101 gtcgagcggg ttgaggacta tctgaccgat ggccaggagc taaaggtccg ggttcgtgag
     2161 atcgatcggg gcaaggtcag cctcgagttg atcgaagccc tggagggcgc gacgttgccc
     2221 gagtccgagc caccgagctc gggtggtggt gaccggggcc ggggttccgg tgaccgtgat
     2281 cgtggccgtg gaggctcggg cgatcgggga cggggcggct ctggggatcg tgaccgcggc
     2341 cgtgaccaga accgagacga ccgtccggct ccggagaagg ctcccgtggc catcgcccaa
     2401 ccgacttcgg ccagctccga gcgtcgtcgc gtggcccagc cattcgacga ggtcttcgaa
     2461 gacctccaga actagttagt gaagcggcct gttcagggcc gcttccaaaa gcggagctcg
     2521 gcggtctggg tgaacccgac cgccgagtag aacccaatcg catccgagcg gtagtcgtcg
     2581 gtgatcaccc tgacccgggt cattccggct tcgcgcatct ttgagaagcc gtagtt
//
LOCUS       DBNHXU010000199         3084 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3436441, whole genome shotgun sequence.
ACCESSION   DBNHXU010000199 DBNHXU010000000
VERSION     DBNHXU010000199.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3084
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3436441"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..581)
                     /locus_tag="AC161X_05030"
     CDS             complement(<1..581)
                     /locus_tag="AC161X_05030"
                     /inference="COORDINATES: protein motif:HMM:NF013188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter domain-containing
                     protein"
                     /protein_id="HIR4988946.1"
                     /translation="MIGLVILAAVFVCYALVAARLDRWSITAPIVLVLTGALVGLGTP
                     DWLAVIAEPELVKHIAELTLALLLFADASTLRWDQLREDGGLPTRLLLIGFPLTVLAG
                     MGIGAWLYPNAGWAPVALAASILAPTDAALGLGVFTNRAVPGRIRRALNVESGLNDGM
                     ATPLVTLFLAQLIAQEGGGTDHWVSESLRQLAIG"
     gene            complement(693..1481)
                     /locus_tag="AC161X_05035"
     CDS             complement(693..1481)
                     /locus_tag="AC161X_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012932669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4988947.1"
                     /translation="MSTSYAVHDSMTMLRRQFVHMRRYPVTIFLVVVIPIVMLLLFVY
                     VFGATLGAGLSAGSGGRAEYVNYVVPGILLMAVALSAQGPAISVAMDMSEGIIARFKT
                     MAIARVSVLSGHVLGNVISTMLGLVVLIGVALLIGFRPSANPMEWLAAIGVLAMVSYA
                     VTWFSVGCGLAAKTVESASNLPTPLILLPFLGSGFVPTESMPPVLGWFAEFQPFTPFG
                     ETLRALLMGTPMGNSGLLTVAWCVAITLVCYLWSKRLYNRDPSP"
     gene            complement(1478..2437)
                     /locus_tag="AC161X_05040"
     CDS             complement(1478..2437)
                     /locus_tag="AC161X_05040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014178134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4988948.1"
                     /translation="MNTSSSTAAIVTRGLRKSYGDNLVLDGVDLEVPEGTIFALLGPN
                     GAGKTTIIQILSTLIGADGGEVRIAGHDPAADPDAVRKAIGVTGQFSAVDNFLSGEEN
                     MILMADLHHLGKSEGRRRAEELLEQFDLVEAAKNLPSTYSGGMRRRLDLAMGLVGAPK
                     IVFLDEPTTGLDPRGRQVMWQMIRNLVAGGVTIFLTTQYLDEADKLADRIAVLDRGKL
                     VAEGTPDELKKLAPGGHISLQFNGAAELESAASALGEMARDDDNLILQISSDGGVKSL
                     RAVLDRLDHESIEVETLTVHTPDLDDVFFALTGHSTTEKETSS"
     gene            complement(2461..>3084)
                     /locus_tag="AC161X_05045"
     CDS             complement(2461..>3084)
                     /locus_tag="AC161X_05045"
                     /inference="COORDINATES: protein motif:HMM:NF024743.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat-containing
                     protein"
                     /protein_id="HIR4988949.1"
                     /translation="MGKYGSVQITVELPQGSELEAKTSLGDVRVDGRLAATHVRSGAG
                     DVVLDETGPLKVKSGAGAVSVNRAVGNAEITTAGEMHLGAIDGRAQITNQSGNTWIAE
                     VAGPLRVKSHNGDITVGRMEADVAAKTAFGSVLLGEVANGSVSVESAFGGLEVAIREG
                     TAAWVDAKTSFGKVHNRLGSADGPELSEKTVEIQARTSFGDIVIRRA"
BASE COUNT          591 a          963 c          975 g          555 t
ORIGIN      
        1 ccaatagcca attgacgcag cgactcgctc acccaatgat cagtcccgcc gccctcctgg
       61 gcgatgagct gagccaggaa cagcgtgacg agcggggttg ccatcccgtc attgagaccg
      121 ctctcgacgt tgagcgcccg acggatacga cccgggaccg cccggttggt gaacacgccc
      181 agacccaacg cagcatcagt cggggcgagg atgcttgctg ccaatgccac aggcgcccac
      241 ccggcgttcg gatacagcca agccccaatg cccatgccgg caagcacggt cagagggaac
      301 ccgatgagga gcaggcgggt gggaaggccg ccgtcctcac gcagttggtc ccatcgcaat
      361 gtcgaggcgt cggcaaacag caggagtgcc agggtcaact ctgcgatgtg cttcacaagc
      421 tccggttcgg cgatcacggc gagccagtcg ggtgtcccga ggccgacgag agcccctgtc
      481 aggaccaaca caattggagc ggtgattgac caccgatcga ggcgggcggc gaccagggcg
      541 tagcaaacga agacagcagc caggatgacg agcccgatca ttccgctaat gctgcctcgc
      601 gatcacgtga tcgcggtgca attagccgga actgcgagcg tcaggcgcct ttcatgacgg
      661 ctcgaggttg gaatgcggct cgtccaaggt tgtcacggcg aagggtcacg gttgtagagc
      721 ctcttcgacc acaggtagca gaccagcgtg atggcaacgc accaggccac ggtgagcagc
      781 ccgctgttgc ccatcggcgt gcccatcagc agcgcccgca gtgtctcccc gaacggggtg
      841 aacggctgaa actcggcaaa ccagccgagc acaggcggca tcgactccgt gggaacgaac
      901 ccgctgccca ggaacggcag gaggatcaag ggcgtcggca gattgcttgc cgactcgacg
      961 gtcttggccg cgagaccgca cccgaccgag aaccaggtga cggcgtagct gaccatggcg
     1021 aggactccga tcgccgcgag ccactccatc gggttggcac tgggccggaa tccgatcaga
     1081 agggcaaccc cgatcaggac caccaggccg agcatggtcg agatcacatt gccgaggacg
     1141 tgtccgctga gtaccgagac ccgggctatg gccatcgtct tgaaccgggc aatgatgcct
     1201 tcgctcatat ccatggcgac cgatatcgcc ggcccctggg ccgagagggc caccgccatg
     1261 aggagaattc cgggaaccac gtagttgaca tactcggcgc ggcccccgga accggcgctc
     1321 agacctgcac cgagggtggc accgaaaacg tagacgaaca gcaacagcat gacgatcggt
     1381 atcaccacga ccaagaagat cgtgaccggg tagcgccgca tatgaacgaa ctggcgccga
     1441 agcatcgtca tcgagtcgtg caccgcatag gaggtgctca tgaggaggtt tccttttctg
     1501 ttgtggagtg gccggtgagg gcgaagaaga cgtcgtcgag atccggggtg tgcacggtca
     1561 gcgtctctac ttcgattgat tcatggtcga gtcggtcgag cacagctcgc aatgacttga
     1621 cgcccccatc gctggagatc tggaggatga gattgtcgtc gtcacgagcc atctcaccga
     1681 gggcgctggc ggccgactcg agctctgccg caccgttgaa ctgaagactg atgtgaccgc
     1741 cgggggccag cttcttgagc tcgtcggggg tgccttcagc caccagcttc ccgcggtcca
     1801 gcaccgcgat ccgatcggcg agtttgtccg cctcatccag atactgcgtg gtgaggaaga
     1861 tcgtcactcc tcccgccacc aggttgcgga tcatctgcca catcacctga cgtccgcgag
     1921 ggtcgagacc ggtggtcggc tcgtctagga agacgatctt cggggcgccg accaagccca
     1981 tggcgaggtc gagccgacgt cgcattccgc cggagtaggt ggagggcagg ttcttggctg
     2041 cttccaccag gtcgaattgc tcgagcagtt cttctgctcg ccgtcggccc tccgacttgc
     2101 ccagatggtg cagatctgcc atcaggatca tgttttcctc accgctgaga aagttgtcca
     2161 ccgccgagaa ctgcccggtg actccgatgg ctttgcgaac tgcgtcgggg tccgccgccg
     2221 gatcgtgacc ggcgatccgc acttcacccc cgtcggcgcc gatcagagtg gagagaatct
     2281 ggataatggt ggtcttgcct gccccattcg ggccgagcag agcaaagatg gtcccttcgg
     2341 gcacctcgag atcgacgccg tcgagtacga ggttgtcgcc gtatgacttg cgcagacctc
     2401 tggtgacgat ggcggcggtc gatgagcttg tgttcatgat ttcctcgcga tcagatgtgg
     2461 tcaggcgcga cgtatgacga tgtcgccgaa tgaggtgcgg gcctggatct cgacggtctt
     2521 ttccgagagc tcgggaccgt cggctgatcc gagccggttg tggaccttgc cgaagctcgt
     2581 cttggcatcg acccatgcag cggttccttc acggatcgcc acttccagcc caccgaacgc
     2641 ggattcgacc gataccgaac cattggctac ctctccgagc aggacgctgc cgaaggcggt
     2701 tttcgccgct acatccgctt ccatgcgtcc gacggtgata tcgccgttgt gggacttgac
     2761 ccgcagtggc ccggcgacct cggcgatcca ggtattgccg ctttggttcg tgatctgggc
     2821 tcgaccgtcg atcgcaccca ggtgcatctc gcccgcggtt gtgatctcgg cgttgccaac
     2881 tgcccgattc acactgacgg ctccggcgcc ggacttgact ttgagagggc ctgtttcgtc
     2941 gagaacgaca tcaccggctc cgctgcgaac atgagttgct gccagccggc cgtcgacgcg
     3001 gacatcccca agtgaggtct ttgcttcgag ttcggagccc tgcggaagct cgaccgtgat
     3061 ctgaacggag ccgtacttgc ccat
//
LOCUS       DBNHXU010000200         2753 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_263653, whole genome shotgun sequence.
ACCESSION   DBNHXU010000200 DBNHXU010000000
VERSION     DBNHXU010000200.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2753)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2753)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2753
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_263653"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..997
                     /locus_tag="AC161X_05050"
     CDS             <1..997
                     /locus_tag="AC161X_05050"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0019808 - polyamine binding [Evidence
                     IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015846 - polyamine transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="polyamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR4988950.1"
                     /translation="ADCTLDQVDGDLNFYNWTEYMDPALITAFEAEYGIDIVDSYYES
                     NEAMLAQLQAGVNYDLIVPSDYMVGIMIQENLLAPIGRTVVPNMANLGDRFTTLPYDP
                     GNEYSAPYQYGTTGIGVNTSLVGEDFPRSWALLFDPTLTADFPGGVSVLNDPREAMGA
                     ALEYLGYSLNDTDLAHLQEAADVISAAKGTIAAFDSDQNDEALANGEVSVAHGYSGAM
                     IVGFGDAANPDDFVFILPEEGATLWIDNMVVPANAEHPCSAFTFMNYLLDAQNGATLT
                     NFNYYGSPNAAALEFVEPEVVEFYSSAEEGAKLEVIQDTGDYEINFTDYLAIAKG"
     gene            complement(1028..1987)
                     /locus_tag="AC161X_05055"
     CDS             complement(1028..1987)
                     /locus_tag="AC161X_05055"
                     /inference="COORDINATES: protein motif:HMM:NF025616.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4349 domain-containing protein"
                     /protein_id="HIR4988951.1"
                     /translation="MKKLTLILALGLALAACGGDSGETFSEVSSGLDGGGGAVPESMT
                     TVAASAESPDELSYDEEMNSERQVIRNASLELQADDTRSAFEEIVDLIEGAGGFVASA
                     TVEPVDSETDQPVVSMILRVPADELPRVLDAIKDSVEVVMTESQNAEDVTAQFVDLEA
                     RLHNLQALETELVALLAEVREQPDADPAKLLTVFNEVSRVRGEIEQLQGQLNYLNDVV
                     ALATLSIYLSPTPAAVPIVEETWAPLEVARESLRNLVSGLQDIGEWAINFAIYTLPLL
                     LLTLGIPLLVLYLLYRWWKKRRRPAPPAPEPIPPVLPANTQEK"
     gene            2069..>2753
                     /locus_tag="AC161X_05060"
     CDS             2069..>2753
                     /locus_tag="AC161X_05060"
                     /inference="COORDINATES: protein motif:HMM:NF040521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C45 family autoproteolytic
                     acyltransferase/hydrolase"
                     /protein_id="HIR4988952.1"
                     /translation="MSGGPLRVLEIYGDPIDLGRFHGAECADLIRTYLADRLGLAGES
                     TWAGRTAETDLVLTLAEETLPFHNAYSPSLYEEMESLAAAAGITPAEAVVVGGFTDLI
                     DVVRAYTGDAPVEDDCTAVIDPVAKILAQTWDMHASAGDYVVFLKLDPVIGPALVVQT
                     TAGCLGQIGMNEAGISIGINNLTSFGTPGVTWPFVVRKVLQQSNLDDAVNCVLDAHLA
                     GGHNFLLMGP"
BASE COUNT          514 a          842 c          847 g          550 t
ORIGIN      
        1 tgccgattgc accctggatc aggtcgacgg cgacctcaac ttctacaact ggaccgaata
       61 catggatccc gcgctgatca cggctttcga ggcggagtac ggcatcgaca tcgttgattc
      121 ctactacgaa tccaacgagg cgatgcttgc ccagctccaa gccggtgtga actacgacct
      181 gatcgttcct tcggattaca tggtggggat catgatccaa gagaacttgt tggctccgat
      241 cggccgcacc gtcgtgccca acatggccaa tcttggagat cggttcacca cgctccccta
      301 tgacccgggc aacgagtatt cggcccccta tcaatatggg actaccggca tcggggtgaa
      361 cacgtccctg gttggggagg atttcccgcg cagctgggca ttgctgttcg atcccacact
      421 gacggccgat ttcccgggcg gggtttcggt gctcaacgac ccgcgggagg cgatgggggc
      481 ggctctcgaa tacctgggat attcgctcaa tgacacagac ctcgctcacc tccaggaggc
      541 ggccgacgtg atctctgccg ccaagggaac gatcgccgcc ttcgactcgg atcagaacga
      601 cgaagccctt gccaacggcg aggtctcggt ggcacacggg tactcaggcg caatgatcgt
      661 tgggttcggc gatgccgcca accccgacga cttcgtcttc atccttcccg aggaaggggc
      721 gaccctgtgg atcgacaaca tggtggttcc cgccaatgcc gagcatccct gttccgcctt
      781 caccttcatg aactatctgt tggatgccca gaacggggca acactcacca acttcaacta
      841 ctacggcagt cccaacgccg cagctctcga gttcgtcgag cctgaagtgg ttgagttcta
      901 ttcgtccgcc gaggaggggg cgaagctcga ggtcatccag gacaccggtg actacgagat
      961 caacttcacg gactacctgg cgattgccaa gggttagaga acagggctcc ggttggagcc
     1021 ctgttcgtca tttctcctga gtgtttgcag gaagcaccgg tgggatcggc tccggcgccg
     1081 ggggtgccgg ccgccggcgc ttcttccacc agcggtagag gagataaagc acaagcaacg
     1141 ggatgcccag ggtgagaagt agcagcggca gggtgtagat cgcaaagttg atggcccatt
     1201 ccccgatgtc ctgcaggcca ctcaccagat tgcgcaacga ctcacgggcg acctcgaggg
     1261 gagcccaggt ctcttcgaca atcggcaccg cggccggggt cggggagagg tagatcgaca
     1321 gggtggccaa agccactaca tcgttcaagt aattgagctg gccttgcagc tgctcgatct
     1381 cgccgcggac ccgcgagacc tcgttgaaca cggtaaggag cttggccgga tcggcgtccg
     1441 gttgttcgcg gacttctgcc agcagggcca ccaactcggt ctccagggcc tgcaagttgt
     1501 ggagtcgcgc ctcgagatcg acgaattgag cggtgacgtc ctcggcgttt tgggactcgg
     1561 tcatcaccac ctcgaccgaa tccttgatgg cgtcgagaac ccgcggcaac tcgtcggcgg
     1621 gcacccggag aatcatgctc accactggtt ggtcggtctc gctgtcgacc ggctcgaccg
     1681 tggcactggc gacaaagccg ccggcaccct cgatcaggtc gacgatctct tcgaaggccg
     1741 atctggtgtc gtcggcctgc aattcgaggc tggcattgcg gatcacctga cgctcgctgt
     1801 tcatttcttc gtcgtacgac agctcgtccg gcgactcggc cgaggcggca acggtggtca
     1861 tcgattcggg gacggcccca ccaccaccgt cgagaccgga ggagacctcg ctgaaggttt
     1921 cccccgagtc cccgccgcaa gctgcgagcg caagtccaag tgccaggatc aatgtgagct
     1981 tcttcatcat tcgtcctctc gtatcacccc ggagggcttg gcggttggac gcacgcgatg
     2041 tgtcatcggt tcccggtagc gtgtgcccat gagtggcggg ccgcttcgcg tccttgagat
     2101 ttacggtgat ccgattgatc tcggtcgctt ccacggcgcc gagtgcgccg atctgatcag
     2161 gacctatctg gctgaccggc tcggtctcgc cggcgagtcc acctgggcag gccggaccgc
     2221 cgaaaccgac ctggtgctca cactggctga ggaaaccctt cccttccaca atgcctattc
     2281 accgagcctc tatgaggaga tggaatcgtt ggctgcggct gccggaatca ctcctgctga
     2341 ggcggtggtg gtcggtggtt tcacggacct gatcgatgtg gtccgggcat acacggggga
     2401 tgccccggtg gaggacgatt gcacggctgt gatagacccg gtcgcgaaga tcctggcgca
     2461 gacctgggac atgcacgctt cggccggcga ttacgtggtc tttctgaaac tcgacccggt
     2521 tatcggcccg gccctggtgg tccagacgac agcggggtgt ctgggtcaga tcgggatgaa
     2581 cgaggccggg atctcaatcg gtatcaacaa cctgacctca tttggaacac ctggagtgac
     2641 ctggcctttc gtggtacgca aggtccttca gcagagcaac ctcgacgatg ccgtcaactg
     2701 cgtcctcgat gcacacctgg cgggtggtca caacttcctg ttgatgggcc ctg
//
LOCUS       DBNHXU010000201         4440 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3834509, whole genome shotgun sequence.
ACCESSION   DBNHXU010000201 DBNHXU010000000
VERSION     DBNHXU010000201.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4440)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4440)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4440
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3834509"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(166..1854)
                     /locus_tag="AC161X_05065"
     CDS             complement(166..1854)
                     /locus_tag="AC161X_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HIR4988953.1"
                     /translation="MTAVERYVPGVASLRNYERQWLRSDLVAGIVLAAILVPQGMAYA
                     ELAGLPPVNGLYTTIACLIGYALMGPSKILVLGPDSSLGPLIFAAITPLVVAGDDPLT
                     AIALAGVLAILVGLIEVGLGIGKLGFVADLLSSEVQIGYMNGLAITIIVGQLPKLSGF
                     STDADTFVEEVSEFVTNFDQRDATTLTVGLITLAVLLVLPRVTRRIPAVLVAVVGATV
                     VTAVFGLDIATVGTLPKGLPTPEVPWTNMGDVVPLLVAAVGITLVSLTDTIALSTSFN
                     ARRGEQVKPNQEMIGIGSANIAAGFFQGFAVSSSSSRTAVAEQSGAKSQLTGVVGAAV
                     VVLLLLFFNGLLADLPNSALAAVVIAAGLSLTDFSVLFRVWKIRRSAVLLSVVASTGV
                     IFLGVLEGIVVAVVLSILLFFKRNWWPHGEVLGRVSGREGWHSDPEGDGLDEHPNVLV
                     FRWEAPLFFANAGMFAERVRQLVYERRPAWVVLQCEAITDIDVTAAGMLERLDNELNA
                     KGVHLAFVELRTRLRDLVHDYGLLKTLDRDHFYASIEDALSDIDATLGEEKHGP"
     gene            2101..2769
                     /locus_tag="AC161X_05070"
     CDS             2101..2769
                     /locus_tag="AC161X_05070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019180733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4988954.1"
                     /translation="MGESTNESEWISAAEFHRAPGVSDWRVTGTGPQTVFTATSLSQA
                     ADLIAPVVAAAERFGILPDVDVRPEGLVVRIPYRPEGIPSAATEFAAAVSLAAAELLL
                     TPAPALAQSIGIYVAQHSQADVRPFFTAVLGYEAFGDTDAVDPLRCGPQLAFNPVNGD
                     SPARGRTHFDVFVPADQAQARVDAALAAGGRLADDSHAPAWWSLASPDNHGVDIASWT
                     DTYD"
     gene            complement(2833..3432)
                     /locus_tag="AC161X_05075"
     CDS             complement(2833..3432)
                     /locus_tag="AC161X_05075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005407733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR4988955.1"
                     /translation="MIRVVLAEDQAMVRGAFAGLLDLEPDIEVVAVAEDGEQGLAAVT
                     EHQPDILLTDIEMPGMTGLELASRLKDHPTVRVVIVTTFARSGYLRRALDAGVSGYVL
                     KDSPIDDLADTLRRVAAGNRVIAPELAVSAWAGSDPLTDREREVLREVGEGHPNIEIA
                     QRLHLAEGTVRNYLSSAILKLGARNRTEAYAKAQEMGYL"
     gene            complement(3429..>4440)
                     /locus_tag="AC161X_05080"
     CDS             complement(3429..>4440)
                     /locus_tag="AC161X_05080"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4988956.1"
                     /translation="IFSPDAGPEDWLLAVGVVAVALPTFVVSEIRGWDIGGATVLMVL
                     ALATAPFDSTAIMALPIYAAALLARSATQETLRFRLSVITVLTLTGFFLSSIPWPFRI
                     FIFIPVIMIWVIGSAVFHDVTRDHHAVALRAENRRVAHLATIAERERIARDVHDIAGQ
                     ALTAIILRSQLVQKLASSDQRRATEEAGEVEHIARDALSSIRSSVAGWHQASLADELA
                     VARAALAAGGTELVTLGDFEVDLAPSVENVLALGLREAVTNVLRHAKAGTVEARIDSQ
                     DGGVSLTVTDDGIGPTKGTGSGIQGMRERVIAAGGTLTFGPGVTGGTELRIDMPFGGS
                     SS"
BASE COUNT          823 a         1419 c         1432 g          766 t
ORIGIN      
        1 cgcgagagca cggtcggcgc ggccgccgac aactgagagt ccctctgcgt gtcatccgct
       61 ggtgaggatc cgcctagcga ggaacgctgg tcggtgttgc ccgctcgacc aagcggcgat
      121 ccgaccggtt ctgacgctga cccatgacgg tccagccgcg aagtctcatg gcccgtgctt
      181 ctcctcgccg agggtcgcgt cgatgtcgct gagggcgtcc tcgatcgagg cgtagaagtg
      241 gtctcggtcg agtgttttga ggagcccgta atcgtgaacg agatcacgaa ggcgggtgcg
      301 cagttcgaca aaagcgagat gcacgccctt ggcgttgagc tcgttgtcga ggcgctcgag
      361 cattccagcc gctgtgacgt cgatgtcggt gatggcctca cactgcagca ccacccacgc
      421 cggacggcgc tcgtacacca gctggcggac ccgctcggcg aacatgcctg cgtttgcgaa
      481 gaacaggggc gcctcccacc ggaacaccag cacgttgggg tgctcatcta agccgtcgcc
      541 ttcggggtcg ctatgccacc cctcacggcc gcttacacgt ccgagtactt cgccgtgcgg
      601 ccaccagttt cgtttgaaga aaagcaggat cgacagcaca acggccacga cgatgccctc
      661 gaggactccg aggaagatga caccggtgct cgcaacgacc gagagcagca ccgcagaccg
      721 tcggatcttc cacactcgga agagaaccga gaagtcggtg agggagaggc ccgcagcgat
      781 gaccactgcg gccaacgcgg agttcggcag gtctgcaagg agtccgttga agaacaagag
      841 caagagcacc acgacagcgg cgccgaccac cccggtgagc tggctcttgg ccccggactg
      901 ctcggcgaca gccgtgcgtg agctgctgga tgaaacggcg aacccttgga agaacccggc
      961 tgcgatgttg gccgagccga tgccgatcat ctcctggttc ggcttcacct gctcgccacg
     1021 gcgggcgttg aacgaggtgg ataaggcgat ggtgtcagtc agtgacacca gcgtgatgcc
     1081 aaccgcggct acgagcagcg gcacgacgtc gcccatgttg gtccatggca cttcgggtgt
     1141 gggcaacccc ttcggaagcg tgccaaccgt ggcgatgtcg aggccgaaga ccgcggtgac
     1201 aacggtggcg ccaaccaccg cgacgaggac ggcggggatc ctccgcgtga cacgtggaag
     1261 cacgagcaga accgcgagcg tgatgagacc cacagtgagg gtcgtggcgt cacgctggtc
     1321 gaagttcgtg acgaactcgc tgacctcctc gacgaaggtg tcagcgtccg tcgagaaccc
     1381 gctgagcttg ggcagctgcc caacgatgat ggtgatggcc agtccgttca tgtaaccgat
     1441 ttggacctcg ctcgagagca ggtcggcgac gaaaccgagc ttgccgatac ccagcccaac
     1501 ttcgatcagg ccgaccagga tggcgagcac gccggccagg gcgatggcgg tgagggggtc
     1561 gtcgccggcc acgacgagcg gggtgatggc agcgaagatc agcggcccaa gagatgagtc
     1621 ggggccgagt acgaggatct tcgacggccc catgagggcg tacccgatca ggcaggcaat
     1681 cgtggtgtag agaccattca ccgggggcag cccggcgagc tcggcgtacg ccataccctg
     1741 tgggaccagg atcgccgcca ggacgatccc ggccacgagg tcggagcgca accactgccg
     1801 ctcgtagttg cgaagagagg cgaccccggg cacgtatcgt tctacggcgg tcaaccgcgt
     1861 agcgctctca gccattgagc cacctgggtc ctcgagaatt tgaggagcgt actcaagccg
     1921 cttcacagtg atcggacttg atcgctttcc ttcccgacgc catgccggct gcgtaacggc
     1981 gaagtcgaaa cagcgggccg gcgacatctg cgcgccgcga cctcggagta ccgtggagtg
     2041 cgcgcggggt cctcgctcgt cagcggcctg ggactctgac agcttgcgaa ggagttcccc
     2101 atgggcgagt cgacgaacga atcggaatgg atctccgcgg ccgagttcca cagagcgccg
     2161 ggcgtctccg actggcgtgt cacaggcaca ggcccgcaga ccgtcttcac cgcgacctcc
     2221 ctctcccaag cggcagacct catcgccccg gttgtcgccg ccgccgagcg attcggcata
     2281 ctgcccgacg tcgacgtgcg tcccgaggga ttggtcgtgc gcatcccgta ccgcccggag
     2341 ggaatccctt cggccgccac ggagttcgct gccgccgtgt ccctggcagc cgccgagctg
     2401 ctgcttacgc ccgctcctgc cctcgcgcag tcgatcggca tctacgtcgc ccagcactcc
     2461 caggccgacg tgcgaccgtt cttcacggcc gtgctgggct acgaagcgtt cggcgatacg
     2521 gatgccgtcg atccgctgcg ctgcggacca cagctcgcct tcaacccggt caacggcgac
     2581 tccccggcca gaggccggac tcatttcgat gtcttcgtgc cagccgacca ggctcaagcg
     2641 cgggtggatg ccgcactcgc cgccggaggg agactcgccg acgattcgca cgctcccgcc
     2701 tggtggtcgc tcgcgtcgcc cgacaaccac ggcgtggaca tcgcgtcctg gaccgacacc
     2761 tacgactgac tgtgacgcgg cctgtctagg gcatgggtga cgtcggtgac ggtcccagga
     2821 gccatgcctg ggctacaagt aacccatttc ctgggccttg gcgtacgcct cggtgcggtt
     2881 tctggcccca agcttgagga tggcggacga cagataattt cggacggttc cttcggcaag
     2941 gtggagtctt tgcgcgatct cgatgttggg atgtccttca ccgacctctc ggaggacttc
     3001 tcgctcccgg tcagtgaggg ggtcgctgcc ggcccatgcc gaaaccgcga gctccggggc
     3061 gataactcgg ttgcctgctg ccacccgtct gagggtgtcg gcaagatcgt cgatcggtga
     3121 gtctttgagg acatacccag aaaccccggc atcgagcgcc cgtcgcagat agccgcttcg
     3181 ggcgaaggtg gtcacgatta cgactcggac cgtcggatgg tccttgagac gagaagccag
     3241 ttcgagtccc gtcattccag gcatttcgat gtcggtgaga aggatgtccg gctggtgttc
     3301 cgttacagct gccaaaccct gttcgccatc ttcggcgaca gcgactacct cgatgtccgg
     3361 ttcgaggtcg agtaaccccg caaaggctcc tcgcaccatg gcctggtctt cggcgaggac
     3421 gacgcgaatc atgaagaact cccaccaaac ggcatatcga tccttagctc tgtcccgcct
     3481 gtcaccccgg gtccgaacgt gagggttccc ccagcggcga tcacacgttc gcgcatcccc
     3541 tgaattccag agccggttcc tttggtcggt ccgatcccgt cgtcagtcac ggttagcgat
     3601 actcccccgt cctggctgtc gattcgggcc tccaccgtgc cagctttggc atggcgcaga
     3661 acattggtga ccgcctcgcg aagtcccagc gccaggacgt tttcgaccga aggtgccaga
     3721 tcgacctcga agtctccgag cgtcaccaac tcggtgcctc ctgctgcgag cgcagcgcgg
     3781 gcaacggcta gttcgtcggc cagcgacgcc tgatgccatc cagcaacgct gctcctgatc
     3841 gaagacagtg catcgcgagc tatgtgttcc acctcaccgg cttcctccgt ggcgcgacgc
     3901 tggtccgacg aggccagctt ttgcacgagt tggcttcgca agatgatggc cgtgagggcc
     3961 tggcctgcga tgtcgtggac gtcgcgagcg atccgttccc gctcggcaat ggtggcgaga
     4021 tgagcaactc ggcggttctc agcccggagc gcgaccgcgt gatggtcccg tgttacgtcg
     4081 tggaagacag cggatccaat gacccagatc atgatcacag ggatgaatat gaagatccga
     4141 aacggccaag ggatggagct caggaagaag ccggtcagtg tgaggacggt gatcaccgac
     4201 agtctgaatc ggagcgtctc ctgagtggct gacctcgcaa ggagagcggc tgcgtagatc
     4261 ggcagcgcca tgatcgctgt cgagtcgaag ggtgctgtcg cgagtgccaa caccatcagg
     4321 acggttgctc ccccgatatc ccaacctcgt atttccgaga cgacaaaggt agggagggcg
     4381 acggcaacca cgccgacagc caggagccaa tcctcgggac ccgcgtcggg ggagaagatc
//
LOCUS       DBNHXU010000202         3209 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2047978, whole genome shotgun sequence.
ACCESSION   DBNHXU010000202 DBNHXU010000000
VERSION     DBNHXU010000202.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2047978"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="AC161X_05085"
     CDS             <1..525
                     /locus_tag="AC161X_05085"
                     /inference="COORDINATES: protein motif:HMM:NF012566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D-related
                     protein"
                     /protein_id="HIR4988957.1"
                     /translation="NEIILARTKGIGVLPVEIATSFGVSGPNLRASGVPFDLRKVEHY
                     LPYDKFDFEIPIGENGDCWDRWWVRLAEIRQSARIIQQAIDGIPSGPLQAKVPKVIKV
                     PKGETYVRAENPKGEMGYYLVSEGGQGPYRLKIRSASFSNLSILPWILEGVLVPDIIA
                     IMGSLDFVLGDVDR"
     gene            522..1529
                     /gene="nuoH"
                     /locus_tag="AC161X_05090"
     CDS             522..1529
                     /gene="nuoH"
                     /locus_tag="AC161X_05090"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013494741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HIR4988958.1"
                     /translation="MSSFLTDLFSNFFVSLAGKLLLVALMIPLIGMAIGFAEMKLSAK
                     MQARVGPYFAGGRWGWAQLIADGVKFFQKEDLIPEDADRPVFKFAPVLVLVGTVSLFV
                     VVPFGPGLIFRDLSVGIFFALAVSSLSTIGILMAGWSSANKYSLMGGIRAAGQLIAYE
                     LPLILAVVGSVILAGTMSMEGIVQAQIEAGYPYVIIQFVGFGIFMVAALAELSRIPFD
                     MPIAESELVMGYLTEYSGFRFLFFFLAEFASMFTLSAIAVTLFLGGYWLPGVPESALE
                     FAGPVILMAKVFLLVFAMIWFRWTFPRFREDQLQSLAWKWLIPLALLNIAITAVFKVV
                     F"
     gene            1529..2029
                     /locus_tag="AC161X_05095"
     CDS             1529..2029
                     /locus_tag="AC161X_05095"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NuoI/complex I 23 kDa subunit family protein"
                     /protein_id="HIR4988959.1"
                     /translation="MTDTATSRKIPGGGMLKGLWVTLSTMFKTILLPKKHLATVNYPK
                     VKETPVPRARGVIALDQDACTVCMLCARNCPDWCIYIEGHKEEQPPSTPGGRIKVRAT
                     LDRFDIDYALCMYCGICVEVCPFDALFWTPEYEYSEVKMGEMMHDMDRLQDWLTTVPD
                     TPGLEK"
     gene            2026..2577
                     /locus_tag="AC161X_05100"
     CDS             2026..2577
                     /locus_tag="AC161X_05100"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J family
                     protein"
                     /protein_id="HIR4988960.1"
                     /translation="MSFSDWLSEPVNWVFAALALVVLGSAIRVVTAKNVVHAALFLVA
                     TLAGTAGLFLILSAEFVAWVLVLVYIGAVIVLFLFGIMITRAPTGYDVSLDSDRKVWP
                     AILSVVTFGLMSWASMQAFGTTMVSNPGVGTDTAVLGTALMGRFVVPFEIVSFVLLAA
                     LIGGITIARRDLTPAEEEEKALV"
     gene            2591..2896
                     /gene="nuoK"
                     /locus_tag="AC161X_05105"
     CDS             2591..2896
                     /gene="nuoK"
                     /locus_tag="AC161X_05105"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009343500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR4988961.1"
                     /translation="MLLNQFLILAAALFALGVYGLLVRRNLVLILLSVELMLNAVNIN
                     LVAFESVLRSELAVGQVFSIFVITVAAAEVGIGLAIVIMIFRNRRSANVDDLSLMRW"
     gene            3030..>3209
                     /locus_tag="AC161X_05110"
     CDS             3030..>3209
                     /locus_tag="AC161X_05110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988962.1"
                     /translation="MSFAVLAAETEHVAEAGVTGGFWVDTAGIMLILPFVAFGLIMIF
                     GKRMKRQGAEIAIGAL"
BASE COUNT          496 a         1005 c          996 g          712 t
ORIGIN      
        1 aacgagataa tcctcgcccg caccaagggg atcggcgttc ttcccgttga aatcgccacc
       61 tctttcgggg tatcggggcc caaccttcgg gcctccggcg tgcccttcga cctgcgcaag
      121 gtcgagcact atctccccta cgacaagttc gacttcgaga tcccgatcgg ggagaacggc
      181 gactgctggg accggtggtg ggtgcgtctc gctgagatcc gacagtcggc tcgcatcatc
      241 caacaggcca tcgacgggat cccgtcgggg ccattgcagg ccaaggtgcc caaggtgatc
      301 aaggtcccca agggggagac ctatgtgcgt gcggagaatc ccaaaggcga gatgggttac
      361 tacctcgtct ccgagggcgg ccagggcccg tatcggctga agatccgatc ggcgagtttc
      421 tccaatctct cgatcctccc ctggatcctc gaaggcgtcc tggttccgga cataatcgcc
      481 atcatgggct cgttggattt cgtgctggga gacgttgata ggtgagctcc ttcctgaccg
      541 acctcttttc caacttcttc gtctccctgg ccgggaagct gctgctcgtc gccctgatga
      601 tcccgctgat cgggatggcc atcgggttcg ccgagatgaa gctctccgcc aagatgcagg
      661 cccgggtagg gccctacttc gccggaggcc ggtgggggtg ggcccagttg atcgccgacg
      721 gggtcaagtt cttccagaag gaggacctga tcccagagga cgccgatcga ccggtcttca
      781 agttcgcccc ggtgctggtg ctggtgggga ccgtctcgtt gttcgtggtt gtgccctttg
      841 gtccgggcct catcttccgg gacctctcgg tgggcatctt cttcgccctc gccgtctcct
      901 cgttgtcaac gatcggcatc ctcatggccg gttggtcatc ggcgaacaag tactcgctga
      961 tgggtggcat ccgggccgcc ggccagctca tcgcgtatga gctgcccctg atcctggccg
     1021 tcgtcggctc ggtgatcctg gccgggacga tgagcatgga ggggatagtc caggcccaga
     1081 tcgaggccgg ttacccatac gtcatcatcc aattcgtcgg gttcggcatc ttcatggtcg
     1141 cggccctcgc cgagttgagc cggatcccct tcgacatgcc gatcgccgag tcggaactgg
     1201 tcatgggtta tctcaccgag tattcgggat tccgcttcct cttcttcttc ctggccgagt
     1261 tcgccagcat gttcaccctg tcggcgatcg ccgtgaccct gttcctaggt ggctactggc
     1321 tcccgggcgt acccgagtcg gccctcgagt tcgccgggcc ggtgattctc atggcgaagg
     1381 tcttcctcct tgtgttcgcc atgatctggt tccgctggac cttcccccgc ttccgtgagg
     1441 accagttgca gagtctcgcc tggaagtggc tgatccccct agctctgctc aacatcgcga
     1501 tcaccgcggt gttcaaggtg gtgttctgat gacggacaca gccacctctc gcaagatccc
     1561 tgggggcggg atgctgaagg gcctttgggt gacgctgtcg acgatgttca agacgattct
     1621 tctgcccaag aagcatttgg ccaccgtcaa ttaccccaag gtcaaggaga cgccggttcc
     1681 ccgggcccgg ggcgtgatcg cactcgatca ggacgcctgc accgtttgca tgctgtgcgc
     1741 tcggaactgt cccgattggt gcatctacat cgaggggcac aaggaagagc agcctccctc
     1801 gacgcccggc gggaggatca aggttcgggc cactctcgat cggttcgaca tcgactacgc
     1861 cctctgcatg tactgcggga tctgtgtcga ggtgtgcccc ttcgacgccc tcttctggac
     1921 accggagtac gagtactccg aggtgaagat gggcgagatg atgcacgaca tggatcgtct
     1981 ccaggactgg ctgacgacgg tccccgacac ccccgggttg gaaaagtgag cttctccgat
     2041 tggctctccg aacccgtgaa ctgggttttc gccgcgcttg ccctggtagt gctcggctcg
     2101 gcgatccggg tggtcacggc gaagaacgtg gtccacgccg ccctcttcct ggtggcgacc
     2161 ctggccggga ccgcaggcct gttcctgatc ctgtccgccg agttcgtggc ctgggtcctg
     2221 gtcctggtct acatcggagc cgtgatcgtc ctcttcctct tcggaatcat gatcacccgc
     2281 gccccgaccg ggtatgacgt gagcctcgac agcgatcgga aggtgtggcc ggcgattctc
     2341 tcggtcgtga cctttgggtt gatgagttgg gcctcgatgc aggctttcgg cacgacgatg
     2401 gtgagcaatc ctggcgtggg gaccgacacc gcggtcctcg gcaccgcatt gatgggacgc
     2461 ttcgtggtcc cgttcgaaat cgtttctttc gtgttgctcg cagcgttgat cggtggcatc
     2521 accatcgctc gtcgggacct gaccccggcg gaagaagaag agaaggcgct cgtatgagtg
     2581 agggtcacag atgcttctga atcagttcct gatcttggcc gccgccctct ttgccctcgg
     2641 ggtctacggc ctcttggtgc gacgcaacct ggtgctgatc ctgctctccg tcgagctcat
     2701 gctcaacgcg gtgaacatca acctggtcgc cttcgagtcg gtccttcgct ccgagctcgc
     2761 ggtcggtcag gtgttttcga tctttgtgat caccgtggcc gcagccgagg tcgggatcgg
     2821 gctcgccatc gtgatcatga tcttccgcaa ccgccgctcg gccaacgttg acgacctgag
     2881 cctgatgaga tggtgatgac tcgcttgttg ccggccgact ttgcccctcg gcaaccctca
     2941 gccttctgtg gggagctcaa cctgtgcctc acgcctcggg gcgaacagac gccggtcggc
     3001 cggagcttcc gccccgttgg aggtctggcg tgagcttcgc cgttctcgcc gctgagaccg
     3061 agcacgtcgc cgaggcgggt gtcaccggag gtttctgggt cgacaccgca gggatcatgc
     3121 tgatcctccc cttcgttgcc ttcggcctga tcatgatctt cggaaagagg atgaagcggc
     3181 agggcgccga gatcgcaatc ggagccctc
//
LOCUS       DBNHXU010000203         8395 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5025193, whole genome shotgun sequence.
ACCESSION   DBNHXU010000203 DBNHXU010000000
VERSION     DBNHXU010000203.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8395)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8395)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8395
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5025193"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..459
                     /locus_tag="AC161X_05115"
     CDS             <1..459
                     /locus_tag="AC161X_05115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006895326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MraY family glycosyltransferase"
                     /protein_id="HIR4988963.1"
                     /translation="YTVITFWQFRNPQVYESVDPLELAIISAAMFGAILGFLWWNAPP
                     AKIIMGDVGSQAIGGLLAALALLSSTNLLLVVLGAIFVAETISVILQVASFRIFGKRI
                     FRMSPLHYHFDLGGWPETTVVIRFWILSGIGVALGLGFFYGDFLRLEGLL"
     gene            456..1751
                     /gene="murD"
                     /locus_tag="AC161X_05120"
     CDS             456..1751
                     /gene="murD"
                     /locus_tag="AC161X_05120"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HIR4988964.1"
                     /translation="MSRVLVLGAGVSGLAAARLARGRGMSVTMYAEENSPEALSHGFA
                     LATGGWDPVLLTGIDFVVTSPGFSERSLPIVESLEWGIPVWSEIEFAWRHLTKPIAAV
                     TGTNGKTTVTEATSAMLEASGVDSPAAGNIGSPLSDFVDEPSDSLVVEVSSFQLRFTE
                     TFHPVAAAVTNVAVDHLDWHGSEISYRSAKAKVFSNQDESDLLVYDADDEGASSLVSG
                     AASELYPVSGTRLPGGGGGVDGGQLRIGPVEVPIDDLVSVDPVHLVDLAIASALALRM
                     GATPDGVANAATVFSPGAHRRRLVETVDDVAWIDDSKATNPHAAIASIRSHDSVILIA
                     GGLAKGLDPRPLAEEPNVRALIGIGSEGPVLVEAAGQRGHLAVTLERAVELAATLAEP
                     GDTVLLAPGCASFDQFRSYAERGDRFASLVRERAGRRGA"
     gene            1748..2911
                     /gene="ftsW"
                     /locus_tag="AC161X_05125"
     CDS             1748..2911
                     /gene="ftsW"
                     /locus_tag="AC161X_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative lipid II flippase FtsW"
                     /protein_id="HIR4988965.1"
                     /translation="MTANVTSIAKMRAAARDRAERIKVNNRTATTLLVLVVVLVVIGL
                     GATQSASSAVAIEEAQDRFFFFKRQLVGVGIGTAALLIASRVPYLFYRKIAIPFFLVT
                     VALLGAVLVVGPVIGGAQRWLPVAGFSFQPSELAKVAVVFSLAYLFEKKHKLLNRFGH
                     FLVPVVFIVGTVGLLVMKQPDLGTVIVIGAAAMAVILASDAPLRFVALLAISAILVVA
                     VLAFSDDYRADRIVGFLDPWGNSSGEGYQLVQSYYALSNGGIFGVGLGASRARWFYLP
                     NAHTDFIFAIIGEETGLIGALTVIGLFVVLALSGWTVASRAPDKFGRMVAAGITVWLS
                     FQALVNIGGVLGVLPITGIALPFISFGSTALIASMAALGVLVNIAQAGVHRKA"
     gene            2908..3987
                     /locus_tag="AC161X_05130"
     CDS             2908..3987
                     /locus_tag="AC161X_05130"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - obsolete lipid glycosylation
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HIR4988966.1"
                     /translation="MSFLIAAAGTGGHVYPGLAVGEALLDLGVAKDEILYVGGDRMEA
                     KVYPEAGFPYLQVEMRGLQRSASAQNLSLPRVVWRARDLIAATMAERKVHAALALGGY
                     ITVPTGLAARQTHLPLMVAEQNAEAGLANRVASRWAVRSFASFPDTPGLSRSEWVGNP
                     VRRSIAEFDRIELRSQAVAYYSLASDLPTLGVFGGSLGARALNDAVTAMIGAWTGGRM
                     QVVHVTGPSHLEGLVSSPSPGDVTWRRVGFEDRMDLFYAASDLVVGRAGGGIAELTAT
                     GTPAILVPGEFGARGHQAANAAYLAGAGAAILLPEAELTSLGEVVTSIMFDPDVIHEM
                     SEASRKIGKPDAAKAIASAMIEVAA"
     gene            3984..5342
                     /gene="murC"
                     /locus_tag="AC161X_05135"
     CDS             3984..5342
                     /gene="murC"
                     /locus_tag="AC161X_05135"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR01082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008763 - UDP-N-acetylmuramate-L-alanine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HIR4988967.1"
                     /translation="MRLDSDRIHLVGAGGAGMSAIARVLIGMGHHVSGSDLRGGSTLD
                     RLADVGVETHTGHHPEVVATADLVVASSAVPDDDPELAAASIAGISIWRRPRLLEEMT
                     HLWPTIGATGTHGKTTTTALLITALRAAGVDPSFIVGGDLADLGTNGHYGTDPLLVLE
                     ADEAFRTFESLALVGLVVTNVEPEHVEHFGSSEELLETFVGVAASVEGPVVACLDDDG
                     SAEVARRVGAVTYGLDPAAMWRITDFTPAAGGSRFTLSGPGRSAEVQLRRPGLHNARN
                     AAGALALLSTLGYDLQACVHGLGEFHGVGRRWEHRGVVAGVTLIDDYAHHPTEVAATL
                     SASAGVVPGRVWAVFQPHLYSRTERLQDEFGEALSAADVVVVTDVYGSREEPVPGITG
                     ELVAEAVGRRGGEVHYVAHKADLARFLVDRVSEGDLVLTMGAGDITLLPSELASMLAG
                     QR"
     gene            5339..6238
                     /gene="murB"
                     /locus_tag="AC161X_05140"
     CDS             5339..6238
                     /gene="murB"
                     /locus_tag="AC161X_05140"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: protein motif:HMM:NF010480.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008762 - UDP-N-acetylmuramate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HIR4988968.1"
                     /translation="MSTLEELAAAGVVARDVPLGPYTTYKSGGPARYFAVCDDIAAIA
                     ALTETGELGKIPTLVLGRGSNLVVADAGFDGMVIRLGPAFGGVDIDGSQVTAGSAVSL
                     PLLARATVNAGRTGLEFFVGVPGTVGGAVRQNAGCFGLETKDRLITASIIDLRDGARS
                     TLGPEELDLSYRHSSLRPTDLVTEAVFHLEDGDVEAGLQELRRITRWRRDNQPGGTFN
                     AGSVFKNPGPVAAGELIDSLGLKGLSVGDVSVSTKHANFFVAGPEATSADILRLVNLV
                     KDRVLEQTGTILEPEIQFVGFEE"
     gene            6235..6978
                     /locus_tag="AC161X_05145"
     CDS             6235..6978
                     /locus_tag="AC161X_05145"
                     /inference="COORDINATES: protein motif:HMM:NF020067.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HIR4988969.1"
                     /translation="MSIDPRLKERRRVVAEDRARRKVSRLLKLMVAIVIGGLVAWVLL
                     SPWLSVSEVATTGVVSSDTYQTLTEVGAVAGTPMIFVQPGRVEAALLTDPFVGSASVV
                     RDWPDRLIVQIEERTALAWVETADGWAHRAVDGVALPSPESPDDALAWVQLPDLPGGD
                     AEASRDVLGGLAFVQALPESLRIGTTIKMRVNGEFWGTVSGYEVRLGRGVEMEAKARS
                     LAALLAEKPPRGSILTLIAPAHPAVTPPS"
     gene            7082..8131
                     /gene="ftsZ"
                     /locus_tag="AC161X_05150"
     CDS             7082..8131
                     /gene="ftsZ"
                     /locus_tag="AC161X_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013127260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HIR4988970.1"
                     /translation="MSSASGGYLAVIKVVGIGGGGVNAVNRMVEAGVRGVEFIALNTD
                     AQALLMSDADVKLDIGREVTRGLGAGANPEIGRAAAEEHRAELEDALKGSDMVFITAG
                     EGGGTGTGGAPIVAEVARSVGALTVGVVTRPFGFEGRRRAVYAEQGIEALKDAVDTLI
                     VIPNDRLLDVGDPSAPIAETFRLADDVLMSGVTGITDLITTPGLINVDFADVRTIMAD
                     AGSAVMGIGRATGENRAKQAAERAISSPMLETSMEGAKGVLLSVAGPSEMTLHEATEA
                     ARVVSDHAHEDADIIFGAIVDDDLDQEMKVTVIAAGFELRRGQRNSGGTRPAKTPILS
                     DYDEDALDIPTFVQG"
     gene            8128..>8395
                     /locus_tag="AC161X_05155"
     CDS             8128..>8395
                     /locus_tag="AC161X_05155"
                     /inference="COORDINATES: protein motif:HMM:NF014619.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="laccase domain-containing protein"
                     /protein_id="HIR4988971.1"
                     /translation="MIRVPGGDGVAFTEAGDGDQRDDLRSRSLVSASLGIPEEWASLN
                     QIHGNLVHRVDHPGVIGEGDAMWSTVRNLPLAVFTADCFGVVLHA"
BASE COUNT         1338 a         2595 c         2820 g         1642 t
ORIGIN      
        1 tacacggtca tcactttctg gcagttcaga aacccccagg tttacgaatc ggtagatcct
       61 ctggaactgg cgatcatctc cgcagcgatg ttcggggcga tcctcggctt cctctggtgg
      121 aacgcaccac cggccaagat catcatggga gatgtcggct cccaggcgat cggcggcctt
      181 cttgcggcgt tggccctcct ctccagcacc aacctgctct tggtggtcct cggggcgatc
      241 ttcgtcgccg agacgatctc ggtgatcctt caggtcgcgt cgttccggat cttcggaaaa
      301 cgcatcttcc ggatgtcacc actccactac cacttcgatc tcggtggctg gccggagaca
      361 accgtggtca tcaggttctg gatcctgtcc ggaatcggcg tggccctagg cctgggcttc
      421 ttctacgggg acttcctccg cctcgagggg ttgctgtgag ccgggtcctg gtcctgggag
      481 cgggcgtttc ggggctcgct gcagcccggc tggcacgggg ccggggcatg tcggtcacga
      541 tgtacgccga agagaattcg cctgaggccc tgagtcacgg cttcgccctg gcgaccggag
      601 gctgggaccc tgtgctcctc accgggatcg actttgtcgt gaccagtcct ggcttctcgg
      661 agcgctcgtt gcccattgtc gagtcgctgg agtggggcat ccccgtctgg agcgagatcg
      721 agttcgcctg gcggcatttg accaagccga tcgccgcagt taccggcacc aacggcaaga
      781 ccacggtgac cgaggcgacg tccgcgatgc tcgaggcgtc aggagtcgat tcgccggccg
      841 cgggcaatat tggctcgcca ctctccgact tcgtcgacga gccctcggac tcgctcgtgg
      901 tcgaggtgtc gtcttttcag ctcaggttca ccgagacgtt ccatccggtg gcggcggcgg
      961 tcaccaatgt cgcggtcgac catctcgatt ggcacgggtc agagatctca tatcggagcg
     1021 ccaaggcgaa ggtcttctcc aatcaggatg agagcgacct tctcgtctac gacgccgacg
     1081 acgagggcgc atcttcgctg gtgagtgggg cggccagcga gttgtacccg gtttcgggaa
     1141 cccgacttcc cgggggcggt ggcggagtcg atggagggca gctccggatc gggccggtcg
     1201 aggtccccat cgacgatctg gtctcagtgg acccggtgca cctcgtcgac ctggcgatcg
     1261 cttcggcgct cgcccttcgc atgggagcaa ccccggacgg agtggccaat gcggcgacag
     1321 tcttctcgcc aggggctcac cggcgtcgat tggtggagac agtcgacgat gtggcctgga
     1381 tcgatgattc gaaggccacc aaccctcacg ccgcgattgc ctcgatccgc tcacatgatt
     1441 ctgtgatcct gatcgccggt ggcctggcga aaggcctgga tccaaggcct ttggccgagg
     1501 agcccaacgt ccgagccttg atcgggatcg gttccgaagg cccagtgttg gtggaagcgg
     1561 caggtcaacg aggtcatctc gcggtgaccc tggaacgagc ggtcgagttg gcggccaccc
     1621 tggccgagcc gggagacacg gttctcctgg cgccaggctg cgccagcttc gatcagttca
     1681 ggtcgtatgc ggagcgaggc gatcgattcg cctcattggt cagggagcga gcaggaaggc
     1741 ggggagcatg acggcgaacg tgacttcgat cgccaagatg agggctgcgg cgcgtgaccg
     1801 cgccgagcgg atcaaagtca acaaccgcac cgccaccacc ttgttggtcc ttgtcgtcgt
     1861 cctcgtggtg atcgggctcg gcgccactca atcggcttca tcggcagttg ccatcgaaga
     1921 ggcccaggat cgtttcttct tcttcaagcg ccagttggtc ggggtcggga tcgggacagc
     1981 cgccttgctc attgcgagtc gcgtgccgta tctcttctat cgcaagatcg ccatcccctt
     2041 cttcctcgtg accgtcgccc tactcggcgc agtcctcgtg gtgggcccgg tgatcggtgg
     2101 tgctcagcgc tggctcccgg tggcaggttt cagcttccag ccttcggagc tggcgaaggt
     2161 tgccgtcgtc ttctcgcttg cctacctctt cgagaagaag cacaagctgt tgaatcggtt
     2221 cgggcatttc ctggtgcccg tcgtcttcat cgtgggcacc gtcggcctcc tggtcatgaa
     2281 gcagcccgac ctgggcaccg tgatcgtgat cggggccgcg gcgatggcgg tgatcctcgc
     2341 cagcgatgct ccgcttcgct tcgtcgcctt gctggcgatc tcggcgatcc tcgtggtggc
     2401 ggtgctcgcc ttcagcgacg actatcgggc ggaccggatc gtcggcttcc tcgacccgtg
     2461 gggcaattcg agcggcgagg gctaccaact ggttcagagc tattacgccc tgtcgaatgg
     2521 ggggatattt ggagtcggtc tcggggcgag ccgcgcacga tggttctatc tgcccaacgc
     2581 tcataccgat ttcatctttg cgatcatcgg agaggagacc gggctgatcg gggctttgac
     2641 cgtgatcggg ctcttcgttg ttctcgccct ctccggttgg accgtcgcct cccgggcgcc
     2701 ggacaagttc gggcgcatgg tggctgccgg gatcacggtg tggctctcgt tccaggcact
     2761 ggtcaacatc ggcggcgttc tcggtgttct ccccatcacc ggcatcgcac tgccttttat
     2821 ctccttcgga agcaccgccc tgatcgcatc catggcagcc ctgggtgtcc tggtgaacat
     2881 cgcccaggcc ggggtgcatc gcaaggcatg agcttcttga tcgcggccgc aggcaccggg
     2941 ggccacgtct acccgggtct ggcggtcggc gaggctttgc tggacctcgg ggtcgccaag
     3001 gacgagatcc tctacgtggg tggtgaccgg atggaggcga aggtgtaccc cgaggctggt
     3061 ttcccctacc tccaggtcga gatgagaggg ctacagcgtt cggcgtcggc acaaaacctg
     3121 tcgttgcccc gggtggtgtg gcgggcgcgt gatctcatcg ccgcgaccat ggctgaacgg
     3181 aaagtgcatg ccgccctggc cctaggtggg tatatcaccg tccccaccgg gctggccgca
     3241 cgccagaccc atctgccctt gatggttgcg gagcagaacg cggaggcggg tctggctaat
     3301 cgcgttgcct cacgttgggc cgttcgaagc ttcgcttcgt ttccggacac tccgggattg
     3361 agtcgcagtg aatgggtggg aaacccggtg cgccgctcta tcgccgaatt cgatcgcatc
     3421 gagcttcgat ctcaggcggt ggcctattac tcgttggcct ccgatctccc cacgctcgga
     3481 gtgttcgggg gatcgttggg cgctcgagcc ctcaacgatg ctgtgaccgc gatgatcggc
     3541 gcctggaccg gggggcggat gcaagtcgtc catgtcaccg gcccctcgca cctagagggt
     3601 ctggtgtcga gcccgtcccc aggcgatgtg acctggcgcc gcgtcggctt cgaggaccgg
     3661 atggacctct tctacgccgc ttccgacctt gttgtcggtc gggccggagg cgggatagcc
     3721 gaactgaccg ccaccgggac accggcaatc ctggtgccag gggagttcgg agcgcgcggg
     3781 catcaggcgg cgaatgccgc atatctcgcc ggtgccggcg cggcgatcct ccttcctgag
     3841 gcggagctca cctcgctggg ggaggtagtc acttcgatca tgtttgatcc tgacgtgatc
     3901 catgagatgt ccgaggcgtc ccgaaagatc gggaaaccgg atgcggcgaa ggcaatcgcc
     3961 agcgccatga tcgaggtcgc agcatgagac tggactcgga tcgaatccat ctggtgggtg
     4021 ccggtggggc cggaatgagt gcgatcgccc gagttctgat tggcatgggc caccacgtgt
     4081 caggctccga cctgcgcggc gggtcgactc tggatcgact tgccgacgtt ggtgtcgaga
     4141 cccacaccgg ccaccaccct gaagtggttg ccacggccga tctggttgtc gcctcatctg
     4201 cggtccccga cgacgacccg gagctggctg ccgcctcaat agccggaatc tccatctggc
     4261 gtcgtccacg gcttctggag gaaatgaccc acttgtggcc gaccatcggc gctaccggca
     4321 cccatgggaa aacgacgacc accgcccttc tcatcaccgc gctacgggct gccggggtcg
     4381 acccctcgtt catcgtcggg ggcgatctgg ccgacctcgg caccaatggc cactacggaa
     4441 ccgacccgct cctggtgctg gaggccgatg aggcatttcg cacgttcgag agcctcgcgc
     4501 tagtcggcct ggtcgtgacg aacgtcgagc cggagcatgt tgagcacttc ggatcttccg
     4561 aggagctgtt ggagaccttc gtcggggttg ctgcttcggt ggagggccct gtcgtcgcct
     4621 gtctcgatga cgacggttcc gccgaggtgg cgcgacgggt cggggcggtg acctacggcc
     4681 tcgatccggc ggctatgtgg cggatcaccg acttcacccc ggcagccggg ggcagcaggt
     4741 tcacgctgtc gggaccgggt aggagcgccg aagttcagct gcgccgacca ggtctacaca
     4801 acgcccgcaa cgccgctggc gccctcgccc ttctctccac gctgggctac gaccttcagg
     4861 cgtgcgtcca cgggttgggc gaatttcacg gagtaggccg ccgctgggag cacagaggag
     4921 tcgtggccgg agtgaccctg atcgacgatt acgcccatca tccgaccgag gtcgcggcga
     4981 cgctgagcgc gtcagccggg gtagttcccg gacgggtgtg ggcggtgttc caaccgcatc
     5041 tctacagccg gacggaacgg ctccaggacg agttcgggga ggccttgagc gcggcagacg
     5101 tcgtggtggt caccgatgtc tatgggtccc gtgaagagcc ggtgcccggc atcaccgggg
     5161 agctggttgc cgaggcggtg ggccggcggg ggggagaagt ccactacgtc gcccataagg
     5221 cagacctggc caggttcctc gtcgaccggg tgtcggaagg cgacctggtg ctgaccatgg
     5281 gcgcagggga catcaccctg ctcccatcag agctcgcttc catgctggcc gggcagcgat
     5341 gagcacgctc gaagagctcg ctgcggcggg cgtggtcgcc cgtgatgtgc cgttgggccc
     5401 ctacaccacc tacaagtcgg gcgggcctgc ccgctacttc gcggtctgcg atgacatcgc
     5461 ggcgatcgcc gccctgaccg agaccggcga actcgggaag attccgaccc tggtcctggg
     5521 tcgaggctcc aacctggtgg tggccgacgc cggcttcgac gggatggtga tccgcttggg
     5581 ccccgccttc ggtggcgtcg acatcgacgg gtcgcaggtg actgcaggat ctgcggtctc
     5641 gcttccgttg ttggcgaggg ccacggtcaa cgctggtcga accggcctcg agttcttcgt
     5701 tggcgtcccg ggcacggtgg gcggggcggt gcgccagaat gccggttgct tcgggctgga
     5761 gaccaaggac cgcctgataa ccgccagcat catcgatctt cgggacgggg cgcggtcgac
     5821 cctcgggccc gaagagctgg atctctccta ccgacactcc agtctccggc cgacggacct
     5881 cgtcaccgag gccgtgtttc atctcgagga tggggatgtc gaggccggcc tgcaggaact
     5941 gcgacggatc acccgttggc gtcgagacaa tcagcccggg gggactttca atgccggcag
     6001 tgttttcaag aacccgggtc ctgtggccgc aggtgagttg atcgactcgc tcggtttgaa
     6061 agggttgagt gtcggagacg tatcggtatc gacgaaacac gccaacttct tcgtggccgg
     6121 cccggaggcg acatctgcgg acatcctgag actcgtcaac ctggtgaaag accgcgtctt
     6181 ggaacagacc ggcaccatcc ttgagcccga gatccagttt gtgggattcg aagaatgagc
     6241 atcgatccaa gactcaaaga gcgacgccga gtcgtggccg aggaccgagc gcggcgcaag
     6301 gtcagccgac tgctcaagct catggttgcg atcgtgatcg gcggccttgt cgcttgggtt
     6361 ctgctctcgc cgtggctgag tgtgtccgag gtggccacga caggagtggt ttccagtgac
     6421 acctaccaaa ccctcaccga agtcggggcg gtcgccggga cgccgatgat cttcgttcag
     6481 ccgggcaggg tggaagcagc tttgctgact gatccgttcg tcgggagcgc cagcgtggtc
     6541 agggactggc cagatcggct gatcgtccag atcgaggagc ggactgccct cgcctgggtg
     6601 gagactgccg acggctgggc ccaccgcgcc gtcgatggcg tggctctccc ttcgcccgag
     6661 agcccggacg acgcactggc ctgggttcag ttgccagatc tgcctggcgg cgatgctgag
     6721 gcctcacggg acgtgctcgg gggactggcg tttgtccaag ctctaccgga gtcactgcgc
     6781 atcgggacca cgatcaagat gagagtgaac ggcgaattct ggggcacggt gtccggatac
     6841 gaggtccgtc tggggagagg ggtggaaatg gaggccaagg cccgaagtct tgccgccttg
     6901 ttggccgaga agccgccccg ggggtcgatc ctgaccttga tcgcgcccgc ccatcccgcg
     6961 gtcaccccac cgtcatgaaa accctgaagg tgtacttgaa ccttaggagt ttggggttta
     7021 cttaccccga ggcgaccatt aaacctctac ttcagctttc gatttcccct tggaggtatc
     7081 cgtgagttca gcgagtggcg gctatctggc cgtgatcaaa gtggtaggta tcggtggcgg
     7141 tggagtcaat gccgtcaacc ggatggtcga ggccggtgtg cggggtgtgg agttcatcgc
     7201 cctcaacacc gatgcccagg ccctgttgat gagtgacgcc gatgtcaagc tcgacatcgg
     7261 gcgcgaagtc accaggggcc tcggcgccgg ggccaaccct gagattggcc gggcggctgc
     7321 cgaagagcac cgcgccgaat tggaggatgc cctcaagggc tcggacatgg tgttcatcac
     7381 ggctggtgaa gggggtggca ccgggaccgg tggcgctccg atcgtggctg aagtcgcccg
     7441 ttcggtcggc gcactcaccg tcggtgtggt cactcgcccg ttcggcttcg agggccgacg
     7501 tcgcgccgtc tacgccgaac agggaatcga agccctcaag gatgcggtcg acactctcat
     7561 cgtcatcccg aatgaccgcc tcctagacgt tggggacccc agtgcgccga tcgccgagac
     7621 cttcaggctc gccgatgacg tcttgatgag cggggtcact ggcatcactg atctgatcac
     7681 aacccccggg ttgatcaatg tcgactttgc cgatgtgcgc acgatcatgg ccgacgccgg
     7741 atcggcagtc atgggcattg gtcgggctac tggtgagaat cgtgcgaaac aggccgcaga
     7801 gcgggcgatt tcgagcccga tgttggagac ctcgatggaa ggtgcgaagg gcgtcctgct
     7861 ctctgtcgcc ggcccctccg agatgacctt gcacgaggcc actgaggcgg cgcgagtggt
     7921 ttccgaccac gcccacgagg acgccgacat catcttcggc gccatcgtcg atgacgatct
     7981 cgaccaggag atgaaggtca ccgtgatcgc ggccggtttc gagcttcgcc gcggacagcg
     8041 gaacagtggt ggcactcgac cggccaagac tcccatcctc tcggactacg acgaggacgc
     8101 cctcgacatt ccgaccttcg tccagggatg attcgagtcc ccggtgggga cggggtggct
     8161 ttcaccgaag ccggcgatgg cgatcaacgt gacgatctcc gttcgcggtc gctcgtttcg
     8221 gcgtccctgg ggatccccga ggaatgggcg tcgctcaatc agatccacgg gaacctggtc
     8281 catcgagtgg atcaccctgg tgtgattggc gagggcgacg ccatgtggtc cacggttcgc
     8341 aatcttccac tggcggtgtt cactgctgac tgcttcggcg tggtgcttca cgccc
//
LOCUS       DBNHXU010000204         3676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4032700, whole genome shotgun sequence.
ACCESSION   DBNHXU010000204 DBNHXU010000000
VERSION     DBNHXU010000204.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3676
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4032700"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..722)
                     /locus_tag="AC161X_05160"
     CDS             complement(<1..722)
                     /locus_tag="AC161X_05160"
                     /inference="COORDINATES: protein motif:HMM:NF013434.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR4988972.1"
                     /translation="MTDRRLIVLGAGGIGSAVAYWGSKVLGDSVLSLEQFALGHVNGG
                     SEDHSRIIRLSYHTPQYVELAKAAYSAWEQVEVDSGEQLILRTGGLDLAPSGASIGLD
                     EYRSSMTAANVPFDELTAEEVMRRWPQWRLTDDVTALYQDRSGVAMASRANAAHRKLA
                     KEQGANFIEGAEVTAIRETGGEVEVVAGGVAYRAERLVIAAGAWTNRLLTHLDIQFPL
                     EVTQEQVVYLKPLDPLAFHPSR"
     gene            complement(719..1825)
                     /locus_tag="AC161X_05165"
     CDS             complement(719..1825)
                     /locus_tag="AC161X_05165"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007228521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR4988973.1"
                     /translation="MIPTLARDRYVAPEWFAKEQDKVFESQWFCVGRTEEIPEPGDHL
                     VCQVVGESVIVTRARDGGLHGYFNLCRHRGSVLSDAVGKPVQEALGPTGTFHGSIQCP
                     YHAWTYSFDGRLRAAPFLEEDDGLRKEDLPLHAVAVETWGGFLWVHLNPESAAPLAAQ
                     FQGTETYLANYPLDDLRTAIRIVYPVAANWKAIVENYNECYHCGPVHPELVELVPAFR
                     KRGGSDLDWQEGIPHREGAWTFTLSGTTNRQPFPGLSNEEAERHKGQLIYPNLMLSLS
                     ADHVAAFTLWPHAADSTTVVCDFLFHRDEMDRPEFDPMDAVEFWDMVNHQDWRVCQLV
                     QRGMGSRRFTTGFYAPMEDYSMDIRRYLGDLGLQ"
     gene            complement(1872..>3676)
                     /locus_tag="AC161X_05170"
     CDS             complement(1872..>3676)
                     /locus_tag="AC161X_05170"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CocE/NonD family hydrolase"
                     /protein_id="HIR4988974.1"
                     /translation="CTARDWSTYAYLAKHGIAGVRVDIRGTGASNGIIENEYTGQEQA
                     DTLEVMRWLAAQDWCTGDLGMWGISWGGFSSLQTAMLQPPELKAIVPMHATHDRFASD
                     VHYTGGSLHAAEQVDWPTSMVALNGLPPDPDIVGDVWFEEWMQRLEGTPQWPFEWLHH
                     QKRDAYWLHGSPCADYSAIQCPTLLIGGWIDGYVDGMLALAEHLTCPTRTVIGPWGHY
                     RPATGVPGPTLDHLDLMTRWFAHHLRGDQNGIMELPQLAVYVRTGPPYDGVRAAGYWR
                     AEPSWPPVDGSTLAFGLEDLEHDELTWRGPQWVGGHAPAWDRAGIGSLDSSLDDDASL
                     CFETPPMDEPIEILGYPEVEVTVTSDRAVGLVAARLLTVSPSGETHLICRGNRNLAFP
                     DDLSSPVLVEPGKAVTVRFALLAASAVIPRGWRLRLALAGADFPVVWPPGERFAVTID
                     PSASKLLLPVVPPRSSTQDLDWQESAPPPPPPTLATNSDRDWAISHEGDTTRFRRDIE
                     SREEQPERADLIYDSQQSWEVVVGDEDPATTRVWSQNEIHLERPGWSVGTVGTLEMTA
                     DAHTFHLTVELKAMHDGSLIWERRWIEQIPREWA"
BASE COUNT          640 a         1160 c         1198 g          678 t
ORIGIN      
        1 aaccggctcg ggtggaacgc gagggggtcg agtggcttca gatagaccac ctgctcctgg
       61 gtgacctcga gtgggaactg gatgtcgagg tgggttagga ggcgatttgt ccaagcccct
      121 gctgcgatca ccagcctctc ggcccgatag gcgacacccc cggccaccac ctcgacctcg
      181 cctccggttt ccctgattgc ggtcacctcg gcaccctcga tgaagtttgc tccctgctcc
      241 ttggccagct tgcgatgtgc tgcgttcgcc cgggatgcca tcgcaacgcc cgatcgatcc
      301 tgatacaagg cagtgacgtc gtcggtgagc ctccattgcg gccagcggcg catgacctcc
      361 tcagcagtca gctcatcgaa gggcacattt gccgcggtca tcgaggaccg gtactcgtcg
      421 aggccgatcg aggctccact cggcgccaga tcgagccctc ccgttctcag gatcaactgc
      481 tctcccgaat ccacctccac ctgttcccag gcggaatagg cggctttcgc caactcgacg
      541 tattgggggg tgtgatagga gaggcggatg atccggctgt ggtcctcgga cccgccgttc
      601 acgtgaccca acgcgaattg ctccaggctg agcacggagt cgccgagcac cttggatccc
      661 caataggcaa ctgcgctgcc gatgccacct gcacccagga cgatgagacg gcgatcggtc
      721 actggagccc gagatcaccc aggtagcgcc gtatgtccat tgagtaatcc tccatcgggg
      781 cgtagaagcc tgtggtgaac ctccgcgatc ccatccctcg ctggaccagc tgacagacgc
      841 gccaatcctg gtggttgacc atatcccaga actccacggc gtccataggg tcgaactccg
      901 gtcgatccat ctcgtcgcga tggaagagaa aatcgcagac cacggtcgtc gaatcggcgg
      961 cgtggggcca cagcgtgaaa gccgcaacat ggtctgccga gaggctcagc atcaggttgg
     1021 ggtagatgag ctggcccttg tgccgctcgg cttcctcgtt ggacaggcca gggaacggct
     1081 gccggttggt ggttccgctc agggtgaagg tccacgcgcc ttcacggtgt gggatgccct
     1141 cctgccagtc gaggtcggaa ccgccccgtt tcctgaaggc ggggacgagc tccaccagtt
     1201 cggggtggac cgggccgcag tggtagcact cgttgtagtt ctcgacgatt gccttccaat
     1261 tggcggcgac cgggtagacg atcctgatgg cggttcggag gtcgtccaat gggtagttgg
     1321 ccaggtaggt ctctgtcccc tggaactgtg ctgccagagg cgctgcagac tcggggttga
     1381 ggtggaccca gagaaagcct ccccaggtct cgacggcaac ggcatgcaaa ggaaggtctt
     1441 ccttccgaag cccgtcatcc tcctccagaa atggcgcggc tctgagacgc ccgtcgaatg
     1501 agtaggtcca tgcgtgatac gggcactgaa tcgagccgtg gaaggtgcct gtcgggccca
     1561 gggcctcctg caccggcttg ccgaccgcgt cgctcagcac cgatccgcga tgccggcaga
     1621 ggttgaagta cccatggaga ccgccgtcgc gggcccgggt gacgatcaca gactcgccaa
     1681 ctacctgaca caccaggtgg tccccaggct ccgggatctc ctcggtgcga ccgacacaga
     1741 accactggct ctcgaacacc ttgtcctgct ctttggcaaa ccattcgggc gccacatagc
     1801 gatcgcgggc cagggtgggt atcaccgggt tgagattacc cgagccgctg gttcggacca
     1861 gatgggtgcg atcaggccca ttccctcggg atctgctcga tccagcgtcg ctcccagatc
     1921 agagagccgt catgcatggc cttcaactcc acggtgagat ggaaggtgtg ggcgtctgcg
     1981 gtcatttcca atgtgccgac cgtcccgacg ctccagccgg gtcgctcgag gtggatctcg
     2041 ttctgggacc agacccgtgt cgtcgcgggg tcttcgtcgc cgactaccac ctcccaactc
     2101 tgttgggagt cgtagatcag gtccgccctt tcgggctgct cttctctgct ctcgatgtcc
     2161 cggcgaaacc gcgtggtgtc accttcgtga ctgatggccc agtcgcggtc tgaattggtc
     2221 gccagggtgg gtggtggcgg gggcggggct gattcctgcc aatccaggtc ctgggtggag
     2281 ctgcggggag gtacgaccgg taacaggagc ttcgaggcgc tggggtcgat tgtcacggcg
     2341 aagcgctcgc ccgggggcca gaccacagga aagtcagcgc cagccaaggc gagacgcaga
     2401 cgccatccgc gggggatgac cgcggaggca gccagcaggg caaagcggac cgtcaccgcc
     2461 ttgccgggct cgacgaggac cggggaagaa agatcgtcgg gaaaggccag gtttcggttg
     2521 ccccggcaga tgagatgggt ctctccgctt ggcgacaccg taagcaggcg tgcggcgacg
     2581 agtcccacag cgcgatccga agtcacggtc acttcgactt cgggatagcc gaggatttcg
     2641 attggctcat ccatcggtgg cgtctcgaag cacaacgagg catcgtcgtc gagggacgaa
     2701 tccagactgc cgattccagc gcggtcccac gccggggcat ggccaccgac ccactgcggt
     2761 ccccgccagg tcagctcgtc gtgctccagg tcttcgagtc cgaaagccag ggtgctcccg
     2821 tcgacgggtg gccacgaagg ctccgccctc cagtagccgg cggcccggac cccgtcgtag
     2881 gggggccctg ttctcacata gaccgcgagt tgcggcagtt ccatgatccc gttttggtca
     2941 ccacgcaaat ggtgcgcgaa ccatcgggtc atcaggtcca ggtggtcgag ggtgggcccg
     3001 gggactccgg ttgccggccg gtagtggccc cagggaccga tcacggttcg ggtggggcaa
     3061 gtgagatgct cggcgagggc cagcatcccg tctacatagc cgtcgatcca gcccccgatc
     3121 aggagggtcg ggcactgaat cgcgctgtag tccgcacagg gcgagccatg aagccagtag
     3181 gcgtctcgct tttgatggtg gagccattcg aacggccact gcggtgtccc ctcgaggcgt
     3241 tgcatccact cctcgaacca gacatcaccc acgatgtcag gatccggtgg caggccgttc
     3301 agggccacca tcgaggtcgg ccaatcaacc tgctcggcgg cgtgcaacga tccgccggtg
     3361 tagtgcacgt cggatgcgaa ccgatcgtgg gtggcgtgca tcgggacgat ggctttgagc
     3421 tcgggaggtt gaagcatggc cgtctggagc gagctgaacc cgccccacga gattccccac
     3481 atcccgagat ccccggtgca ccaatcctgt gccgcaagcc atcgcatgac ttccagggtg
     3541 tcggcctgtt cctggccggt gtactcgttc tcgatgatgc cgttcgaggc gccggttccc
     3601 cggatgtcga cgcgcacccc tgcgatgccg tgctttgcca ggtatgcgta ggtggaccag
     3661 tcccgggcgg tgcagt
//
LOCUS       DBNHXU010000205         7552 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3635422, whole genome shotgun sequence.
ACCESSION   DBNHXU010000205 DBNHXU010000000
VERSION     DBNHXU010000205.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3635422"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1584)
                     /locus_tag="AC161X_05175"
     CDS             complement(<1..1584)
                     /locus_tag="AC161X_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012892931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4988975.1"
                     /translation="MAGEQESLNTRKRDIRQTRRRLDTWDRIKFLIFLAVVMTAIIAG
                     SDDLGFLSFWDVVVEFFTTTTGKVLGTIFLLECLRQIHYWFSERSAAYNSFWQDSIFG
                     GIQRWAESHFKPWTRFRVGRFFRWALYLFIIGLLVDYFVPEVQGVIQAIVEIPRLIVG
                     ALPFAFQLMFGFFFVAFQFLGIFWLLSRGGMDVILPEEVTTRFDDVWGQDHVLDLIKE
                     NIAFLEKPDEIEKKGGYIPGGILLWGPPGTGKTLMAQAIAGETGRPYVFVEPGAFINM
                     FMGIGVLKVKSLYRKLRKLSLRHGGVIVFFDEADALGSRTGMGSPQGQFGMSEADLGL
                     TCNGFGFMGSQTQAALVQYWNQTAPAAAEDQPKKGWIDRIMMGGMAGGGGGMGTLQAL
                     LTEMNGLTKPRGITNKFRKMFGFRPKPPPKYRILHILATNMPNSLDEAMLRPGRIDRI
                     YKVGYPSKEGRKQTIVGYLEKVDHYLTPEQVDQLAVITPYYSGASIKDLINEALIIAV
                     RAGRESIEWPDVWRAKSLKE"
     gene            complement(1560..2195)
                     /locus_tag="AC161X_05180"
     CDS             complement(1560..2195)
                     /locus_tag="AC161X_05180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988976.1"
                     /translation="MMRHLVVLATATLLISACGGDSDAVITYTDTQRLGRFQIPEQWH
                     TYELSELSSLEDLPFDEPVQGFTFPAVSSIGFDGDPVRDVAHLGRALPESEYPIGLMS
                     VRQIGETQKDFLSRAVLTQSVLPYYDLANSQELQKEDFSFGGGFDGVRVLVAFEDAAG
                     QNQGVAYMITVSNPDDTKMYSMIAGCSRECFISHQETIVGVVDSWLVNKRA"
     gene            2246..2824
                     /locus_tag="AC161X_05185"
     CDS             2246..2824
                     /locus_tag="AC161X_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007450148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HIR4988977.1"
                     /translation="MREISAAARSDVWHDDAVMETHGGKLLVATPQLIDPHFWRTVIF
                     MLQHDEEGTVGLVLNRPTPELVVDHLPEWVTGKNEPQRLFYGGPVEPEMAIALGPGGV
                     GEPTMLPGVTLVDLSTIPPTDPTRIRVYSGYAGWGAGQLEAEIAEGSWYPVQASPDDP
                     FDRPEGQWARILRRQSGYLSLVSTFPEDVALN"
     gene            2853..3557
                     /locus_tag="AC161X_05190"
     CDS             2853..3557
                     /locus_tag="AC161X_05190"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HIR4988978.1"
                     /translation="MTSATAPKLRPSLKRKAPGLEVERSLWAAGAPIVVGVDEVGRGS
                     WAGPLTIGAAVVPSTSRVYKIRDSKMLNEEEREALFDRIAGWCDAWAVGHATPAECDE
                     LGMSDAQRLAARRALDALGVTPDQVIVDGNWDFVGGAKRIVRGDASSLSIAAASILAK
                     VSRDRIMRETADNFPGYNFEANKGYPCPIHKAALQAWGPTSIHRRSWVFMDHLMWNGL
                     TRLQRPEPQLTLEFEV"
     gene            3562..4800
                     /locus_tag="AC161X_05195"
     CDS             3562..4800
                     /locus_tag="AC161X_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X"
                     /protein_id="HIR4988979.1"
                     /translation="MRLSEADLAMLNGEAGPGAQMAMELLTAAAPAARATELIDIVGA
                     HIDGCLYHGVAGLDFARDLAERGARAAVPTTLNVSSIDLIHPDLYRGPEEEARAAREL
                     MDAYVTMGCSPTWTCAPYQLAVRPRFGDQVAWGESNAIVFANSVLGARTNRYGDFLDI
                     CCAITGRAPASGLHTDEGRRATFHLAVRVPDRLLDEELLYPLLGHVLGQEAGTEVAVI
                     SGLDGRADEDRLKSLGAAAASSGAVAMFHVVGVTPEATTLEEAAGGKDPLRTVVVDVD
                     RLNSARKSLEVGSGDLTAVSVGTPHISIRELETLAGLVTGARSKVPFYVNVGRDVLDE
                     ASSQQLVEPITKFGATLVTDTCTYITPIMEDLSGVVMTNSGKWAYYAPGNIGVQVVMG
                     SLGECVQSAVSGQVVRGEPW"
     gene            4797..5207
                     /locus_tag="AC161X_05200"
     CDS             4797..5207
                     /locus_tag="AC161X_05200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase X swivel domain-containing protein"
                     /protein_id="HIR4988980.1"
                     /translation="MTELEYEVLVPGQAEGALIVLEEPLSFWGGFDAETGRIIDNNHS
                     RMGTKLTGSIVAMPHGRGSSSSSSVLAEALRRGTAPAGFILGEPDPILVIGALVAQRL
                     YQASCPILVGPIPVEGAGTWVIDCDKSPPVTKID"
     gene            5207..6292
                     /locus_tag="AC161X_05205"
     CDS             5207..6292
                     /locus_tag="AC161X_05205"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HIR4988981.1"
                     /translation="MTARGYKGPDSHTNQQGDLIRDHIDVGELNAFFKRMPVAFYRSA
                     LNGELVAANPALAVLLGYDTVEELKSQVATVASVYVNPERRLRWIEEIGATGVVYDFD
                     IELRRPNGTTVWARDSARAIRDDAGEVMYYEGSLVDVTDKIEAQKAKDEFIATVSHEL
                     RNPIAAMLGLGQELASNYDTFSDEDRREMAQMIARQADDASWLIEDLLVAYGDNLNGV
                     SVHPEVFDVVKEIERVLEVEDHPIDLQVFDGSSMITADPRRTRQILKNLVSNALRHGG
                     ETVSVALAKVGDRIEVKVRDSGEPIPDSDVERIFKAFEMGSRVAHPNSVGLGLAVARR
                     LARLMEGDLTYRYQDGWSSFVLSLPTA"
     gene            6320..7318
                     /gene="galE"
                     /locus_tag="AC161X_05210"
     CDS             6320..7318
                     /gene="galE"
                     /locus_tag="AC161X_05210"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005473232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HIR4988982.1"
                     /translation="MRVLVTGGAGFIGSHTVISLIEAGHEPVIVDNLSNARRSVVTRI
                     GEITGTMPEFVEADIRDREAMREVVGKGLDACMHFAALKSVGESVEKPLLYYDNNVGG
                     TISLVEALLEFDVKCLVFSSSATVYGEPTVLPLVESLPVGTAVNPYGWTKIMMEQVLR
                     DLQFANPDWSISLLRYFNPVGAHPSGLIGEDPAGDPSNLMPYVARVAAGILPKVRVYG
                     GDYPTPDGTGVRDFIHVVDLAEGHVRALDRHGAESGVFTYNLGTGEGHSVLEVIKSYE
                     QESGRPIPYEITDRRPGDVATSYADVSKAATDLGWKTTRNLAEMCSDSWRWQNQPR"
     gene            7338..>7552
                     /locus_tag="AC161X_05215"
     CDS             7338..>7552
                     /locus_tag="AC161X_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020552651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HIR4988983.1"
                     /translation="MTDILERARVWVAGDPDPETRAVLEAMIESEDTSGLDRAMGGTL
                     SFGTAGIRGEVGPGSNRMNRAVVIRTT"
BASE COUNT         1457 a         2270 c         2418 g         1407 t
ORIGIN      
        1 ctccttgagt gacttggccc gccagacgtc cggccattcg atcgactccc gaccggcgcg
       61 caccgcgatg atcaacgcct cgttgatgag gtccttgatg ctggcccccg agtaataggg
      121 ggtgatcacc gctagttggt caacctgttc cggggtgagg tagtggtcca ccttttccag
      181 gtagccgaca attgtctgct ttcgaccctc cttcgagggg tatcccacct tgtagatgcg
      241 gtcgatccgt cccggccgca acatcgcctc gtccaaggag ttgggcatgt tggtcgccag
      301 gatgtggagg atccgatact tggggggtgg cttgggtcgg aatccgaaca tcttgcggaa
      361 cttgttggtg atccctcggg gcttggtgag tccgttcatc tcggtgagca acgcctggag
      421 ggtgcccatt ccgccgccac cgcctgccat cccgcccatc atgatccggt cgatccagcc
      481 cttcttcggc tgatcctcag cggcggccgg agcggtctgg ttccagtatt ggacgagtgc
      541 tgcctgagtt tgggaaccca tgaaaccgaa tccattgcag gtgagtccca gatcggcctc
      601 tgacatcccg aactgaccct ggggggaacc catcccggtc ctcgagccga gagcgtcggc
      661 ctcgtcgaag aagacgatca caccgccgtg gcgcaggctg agcttgcgca gtttccggta
      721 caggctcttg accttgagaa caccgatacc catgaacatg ttgatgaaag cgccgggctc
      781 cacgaacaca tacggtcgac cggtctcgcc ggctatggcc tgggccatca aggttttccc
      841 ggtgcccgga ggtccccaga gcaagatccc acccgggatg tacccaccct ttttctcgat
      901 ctcatcgggc ttctcgagga aggcgatgtt ctccttgatc aagtcgagga cgtggtcctg
      961 tccccagacg tcatcgaagc gagtggtgac ctcttccggg aggatgacat ccataccgcc
     1021 ccgggaaagc agccagaaga tccccaagaa ctggaacgcg acgaagaaga acccgaacat
     1081 caattggaag gcgaagggga gggccccgac gatgagacgg ggtatttcga cgatcgcctg
     1141 gatgacgccc tggacttcgg gcacgaagta gtcgacgagc agcccgatga tgaacaggta
     1201 gagagcccag cggaagaatc tgccgacccg gaaacgggtc cacggcttga agtggctttc
     1261 cgcccagcgc tggatcccac caaagatgct gtcctgccag aacgagttgt aggcggcgga
     1321 gcgctctgag aaccagtagt ggatctggcg caggcattcg agaaggaaga tcgtcccgag
     1381 aaccttcccg gtggtggtgg tgaagaattc gactaccacg tcccagaaac tcaggaaacc
     1441 gaggtcgtcc gagccggcga taatcgccgt catcacgacg gcaaggaaga tcaggaactt
     1501 gatccggtcc catgtgtcga gacggcgcct ggtctggcga atgtcccgtt ttcttgtgtt
     1561 caggctctct tgttcaccag ccatgagtca acaactccca caatcgtttc ctgatgcgat
     1621 atgaagcatt cccggctaca gccggcgatc atcgagtaca tcttggtgtc gtcggggttg
     1681 gaaacggtga tcatgtacgc caccccctgg ttctgcccgg ccgcgtcctc gaaggcgacc
     1741 agcaccctga ctccgtcgaa tccgcccccg aacgagaagt cctccttctg aagctcctgg
     1801 ctgttcgcca ggtcgtagta gggaagaacg gactgggtga gcacggcccg ggaaagaaag
     1861 tccttttggg tctcaccgat ctgacgaacc gacatcaacc cgatcgggta ctccgattcg
     1921 ggcaaggcac ggccgaggtg tgcgacatca cgcaccgggt caccgtcgaa gccgatgctg
     1981 ctaactgccg ggaatgtgaa tccttggacg ggctcgtcaa acggcaagtc ctcgagcgat
     2041 gagagctctg agagctcgta ggtatgccat tgctcaggga tctgaaaccg gccgagacgc
     2101 tgggtgtcgg tataggtgat cacggcgtcc gaatcgccgc cgcaggccga aatcagcaac
     2161 gttgcggttg ccagcaccac gagatgccgc atcatgcgtg acgacgataa gcgcctgatg
     2221 gcaacagact atccgccgat cggagttgcg ggagatcagt gccgcggccc ggtccgacgt
     2281 atggcacgat gacgccgtga tggagacaca tggcggaaag ctcctcgttg cgacacccca
     2341 gttgatcgac cctcatttct ggcgaaccgt gatcttcatg ctccagcatg atgaggaggg
     2401 aaccgtcggt ctcgtcctca accgacctac cccggagctg gtcgtcgacc atcttcccga
     2461 gtgggtgacg gggaagaacg agccgcaacg ccttttctac ggagggccgg tggagccgga
     2521 gatggcgatc gcactcgggc ccgggggagt gggagagcca accatgctgc caggagtcac
     2581 gctggtcgac ctgtcaacaa tcccaccgac cgatccgacc cggattcggg tctattcggg
     2641 ctacgcaggc tggggagcag gacagctcga agccgagatc gctgaaggat cctggtatcc
     2701 ggtccaggcg tcgcctgacg acccgtttga ccgccctgaa ggccaatggg cgcggattct
     2761 ccggcggcag agtggatatc tctcactcgt gtccaccttt cccgaggatg tagccctgaa
     2821 ttgacgcctc cccgacctac cctccaaaac ggatgaccag cgccaccgca cccaaactga
     2881 ggccaagcct gaagcggaag gccccgggtc tggaagtgga gcgatccctt tgggcagcgg
     2941 gcgccccgat cgtggttggt gttgacgaag tcggacgcgg ctcctgggct ggcccactga
     3001 ccatcggggc tgccgtggtc ccttccacga gcagggtcta caagattcga gattccaaga
     3061 tgctcaatga agaggagcgg gaggccctct tcgaccgcat cgctggttgg tgtgatgcat
     3121 gggcggttgg gcatgccacc cctgcggagt gtgatgagct gggtatgtcg gatgcgcagc
     3181 gcctagcggc tcgccgcgcc ctggacgccc tcggggtgac acccgaccag gtgattgtcg
     3241 acggcaactg ggatttcgtc gggggagcga agcggatagt tcgcggcgat gccagctcgc
     3301 tgtccattgc ggcggcatcg atccttgcca aggtgagccg ggaccgaatc atgagggaga
     3361 ccgcggacaa ctttcccggg tacaacttcg aagccaacaa gggatatccg tgcccgatcc
     3421 acaaggcggc cctccaggca tggggcccta cctcgatcca ccgacgctct tgggtcttca
     3481 tggaccatct catgtggaac gggttgaccc ggcttcaacg accggagccg cagttgacgc
     3541 tcgaattcga ggtctagcca cctgcgcctc tcagaagcag acctcgcgat gctcaacggc
     3601 gaagcgggcc cgggggcaca aatggcgatg gagctgctca ccgcagcagc accggcggcc
     3661 agggctaccg agctcatcga catcgtcggc gcccatatcg atggatgcct ctaccacgga
     3721 gtggccgggc tcgactttgc ccgggacttg gctgagcggg gcgcacgggc cgccgtccca
     3781 accacgttga acgtctcgtc gatcgacctg atacatcccg acctctatcg ggggccggaa
     3841 gaggaagctc gggccgccag agagctcatg gacgcctatg tcaccatggg ctgctcgccg
     3901 acctggacct gtgctcctta tcaactcgcc gtccgccccc ggtttggcga tcaggtggcc
     3961 tggggcgagt cgaatgccat cgtctttgcc aacagcgtcc ttggagcccg gaccaaccgc
     4021 tacggcgact tcctcgatat ttgctgtgcc atcaccgggc gggccccggc gagcggcctg
     4081 cacacggatg agggccggcg agccacattc catctcgcag ttcgtgtccc tgatcggctg
     4141 ctcgacgagg agctcctgta tccgttgctc ggccacgtcc tcggtcagga ggccggtacc
     4201 gaggtggccg tgatcagcgg gctcgacggt cgagccgatg aagaccgatt gaaatcgctg
     4261 ggcgcggcag cggcgtcgtc aggcgcagtc gccatgttcc atgtggtggg agtcaccccg
     4321 gaggcgacca ccctggagga ggcggccggg gggaaggatc cactgcgcac ggtggtagtc
     4381 gatgtcgatc gactcaactc ggcgcggaag tcgctcgagg tcggctccgg ggatctgact
     4441 gccgtcagtg ttggcacccc gcacatctcg atacgtgagc tggagaccct tgccggtctg
     4501 gtcaccggtg cgaggagcaa ggtgccgttc tacgtcaatg tgggccgcga cgtattggac
     4561 gaggcctcga gccaacagct cgtggagcca atcacgaaat tcggagccac gctcgtcacc
     4621 gatacctgca cctacatcac accgatcatg gaggacttgt cgggggtggt catgaccaac
     4681 tccggcaagt gggcctacta cgcgccgggc aacatcgggg tccaggtggt gatggggagc
     4741 ctgggcgagt gcgttcagtc ggcagtttcc ggacaggtgg tcagaggaga gccctggtga
     4801 cagaactgga atacgaggtc ttggtcccgg ggcaggccga gggcgctctg atcgttctcg
     4861 aggagccgct ctccttctgg ggaggcttcg atgccgaaac cggtcggatc atcgacaaca
     4921 accactctcg gatgggaaca aagctcaccg gttcgattgt ggcgatgccc cacggccggg
     4981 gctcgagctc gtcttcctcg gtgctggcgg aggcgttgag acgggggacg gcgcctgcag
     5041 ggttcatcct cggtgagccg gatccgatcc tggtgatcgg cgcattggtg gcgcaacggc
     5101 tgtatcaggc ctcatgtccc atcctggtcg ggccgatacc agtagaaggc gccggaacct
     5161 gggtgatcga ttgcgataag tccccgccgg tgacgaagat tgattagtga cggcccgggg
     5221 gtacaaaggg cccgactcgc acaccaatca gcaaggggac ctcatccgcg accacatcga
     5281 tgtcggcgaa ttgaatgcct tcttcaagcg aatgcctgtg gccttctaca gaagtgcgct
     5341 caacggcgag ctggtggcgg cgaatcctgc ccttgctgtc ctccttggat atgacaccgt
     5401 cgaggagttg aagagccagg tagcgaccgt cgcgagcgtc tacgtgaacc cggagcggcg
     5461 actccgttgg atcgaagaga taggtgcaac tggtgtcgtc tacgacttcg acatcgagtt
     5521 gaggcgcccg aacgggacca cggtctgggc gcgtgattcc gctcgtgcca tccgtgacga
     5581 tgcaggcgag gtcatgtact acgagggatc cctggtcgac gtaaccgaca agatcgaggc
     5641 acagaaggcg aaagacgagt tcattgccac cgtctctcac gagctgagaa acccgatcgc
     5701 agcgatgctg gggctgggcc aggagcttgc cagcaactac gacaccttca gcgatgaaga
     5761 ccgcagggag atggcgcaga tgattgcccg gcaggccgac gacgcctcct ggctcatcga
     5821 ggacctactc gttgcctacg gggacaatct gaacggggtc agtgtccatc ctgaggtctt
     5881 cgatgtcgtc aaagagatcg aacgggtgtt ggaggtcgag gaccacccga tcgaccttca
     5941 ggtcttcgac ggctcttcga tgatcacggc cgaccccaga cgcacacgtc agatcctgaa
     6001 gaacctggtc tcgaacgcac tccgccatgg cggggagacg gtaagcgtcg ccctggccaa
     6061 ggtcggcgac cggattgagg tgaaggtcag ggactccggt gagcccatcc ccgattccga
     6121 cgtggagcgg attttcaagg cgtttgagat ggggagccgc gtggcgcatc ccaattcggt
     6181 cggccttgga ctggcagtgg cccgtcgact ggctcgtttg atggaaggcg acctcaccta
     6241 tcggtatcag gacggttggt cgagtttcgt gttgtcgctc ccgactgcct gagtggcggc
     6301 gacccctaac ctgccccgga tgcgtgttct agtcaccggt ggcgccggat ttatcggcag
     6361 ccacaccgtc atctccctga tcgaggccgg ccacgagccg gtgatcgtcg acaacctgtc
     6421 caacgctcgc cgctcggtgg tcaccaggat cggtgagatc accgggacga tgccggagtt
     6481 cgtcgaggct gacatcagag atcgggaggc gatgcgggag gtcgtcggca aaggcctcga
     6541 tgcctgcatg cacttcgcgg cgctgaagtc ggtgggggag tccgtagaga agccgcttct
     6601 ctactacgac aacaatgtcg gcggcaccat ctctctcgtc gaggctctgc tcgagttcga
     6661 tgtgaaatgc ctcgtcttca gctcctcggc gacggtatat ggagagccga cggtcctgcc
     6721 gttggtcgaa tcgctgccgg tggggacggc cgtcaaccca tatgggtgga caaagatcat
     6781 gatggaacag gtgctgcggg acctgcagtt cgccaaccca gactggtcga tttcgctcct
     6841 ccgctatttc aacccggtcg gtgcacaccc ctcagggctg attggagagg atcccgctgg
     6901 agatcccagc aacctgatgc cctatgtcgc ccgggtggcc gccgggatcc tgcccaaagt
     6961 gagggtctac gggggggact atccaacacc ggacgggacc ggtgtccgtg acttcatcca
     7021 tgttgtcgat cttgccgagg gccacgttcg tgcgctggac cggcatggtg ctgagagtgg
     7081 tgtgttcaca tacaacctcg gcaccggaga aggtcatagc gtgctcgagg tcatcaagtc
     7141 ctacgagcaa gagtccggac gaccaatccc ctacgagatc accgaccggc ggcccgggga
     7201 cgtcgccacc agctatgccg atgtgtcgaa ggcggccacc gacctcggat ggaagaccac
     7261 tcggaacctg gccgagatgt gctccgactc ctggcgttgg caaaaccagc cccgctgacc
     7321 atcggtaccc tcgccctgtg accgacattc tcgaacgagc acgcgtctgg gtcgcaggtg
     7381 atccggatcc ggaaacacgg gccgttctgg aggccatgat cgagtcggag gacacgtctg
     7441 gcctcgatcg ggcgatgggg ggaaccctct ctttcggcac cgcggggatc cgcggtgaag
     7501 tggggccggg atccaatcgg atgaatcgtg ctgtggtgat ccgaaccacc ag
//
LOCUS       DBNHXU010000206         2258 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4628995, whole genome shotgun sequence.
ACCESSION   DBNHXU010000206 DBNHXU010000000
VERSION     DBNHXU010000206.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2258)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2258)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2258
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4628995"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..804
                     /gene="argS"
                     /locus_tag="AC161X_05220"
     CDS             <1..804
                     /gene="argS"
                     /locus_tag="AC161X_05220"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012952.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase domain-containing protein"
                     /protein_id="HIR4988984.1"
                     /translation="LYGTTDLATIQMRIEDLHADLVLYVVDKRQSDHFVQLFRAAQKA
                     GMIPDGTQLEHIWFGTMNGPDGKPFKTREGGVVRLADVIEMLTDAARRRLDEAHLAED
                     YSAEEREQIASMVGIAALKFGDLINNRVSDYVFDLERFSSFEGKTGPYLQYAAVRIKS
                     ILRKAAEAGFGAGRIAPPGVDVERDLMVDLLRLPEVIQRAIDLRSPNHVAEFAYGVAG
                     RFNRFYDACHILSETDPVKQASWLALSAWTLNALESLLDLLGIEIPDRM"
     gene            807..1649
                     /locus_tag="AC161X_05225"
     CDS             807..1649
                     /locus_tag="AC161X_05225"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4988985.1"
                     /translation="MASTMRPARQDDVPEIEAWTRDTFAWGDYVGEGLPTWIEDPSSL
                     VVVCVDEADRPIAMSRSEMLSPTEGWLSAARVHPDHRRAGLGAAMNDFGVEWARERGA
                     KIVRLVIEEDNPAAQAQVAKLGYRNTCVWVRGEANTPRGPLPRERDRLHPASSADADA
                     GWLLWLGSELARAGRELNPGHWRWRKALRSDLDQPLAESSLYQGGAGWVVAVPDGDTL
                     AVGWMVTDPADCPALLEGIAEVARQMGMARIEIFMPETPWSREALVREGFTTRRVLIY
                     VKSL"
     gene            complement(1654..>2258)
                     /locus_tag="AC161X_05230"
     CDS             complement(1654..>2258)
                     /locus_tag="AC161X_05230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988986.1"
                     /translation="GGVTLTESASRTDPLAMVKRYAILAYGAFGTMLDVGLMVLGSLL
                     VGLSIAVLLAGFEIVNVVQELSTGGMLASALVLAIVGLFCLGVASEGPLGRGRKLVGF
                     QLWEVGIGRTVAVFLVGFGALLLYGFIERFLGDLPEPLHKGAEGLRAVGIAGMVIMPL
                     VGVPLSLLIRWAPNAPEWLHKAEVPVLFVVWAIATMAIFT"
BASE COUNT          440 a          750 c          720 g          348 t
ORIGIN      
        1 ctctacggga ccaccgacct ggcaaccatc cagatgagga tcgaggacct gcacgccgat
       61 ctggtcctct acgtggtcga caaacgccag tcggaccact tcgtccagtt gttccgggct
      121 gcccaaaagg cgggcatgat ccctgacggc acgcagctcg agcacatctg gttcgggacg
      181 atgaacgggc cggacggcaa accgttcaag actcgcgagg gtggagtggt ccgcctcgcc
      241 gatgtcatcg agatgctcac cgatgccgcc cgccgccggc tggacgaggc ccatctcgcc
      301 gaggactact ccgccgagga gcgcgaacag atcgcctcca tggtggggat cgccgccttg
      361 aagttcggag acctgatcaa caaccgggtc tcggactacg tattcgacct ggaacgcttc
      421 agctccttcg aaggcaaaac cggcccctac ctccaatacg ccgcggttcg gatcaagtcg
      481 atcctgcgca aggcagccga ggcagggttc ggggcgggtc ggatcgcgcc gcccggggtg
      541 gacgtcgaac gagacctgat ggtcgacctt ctgcgtctcc cggaggtgat ccagcgcgcc
      601 atcgacctcc gctcacccaa ccatgtggcg gaattcgcct acggggtggc aggacggttc
      661 aaccggttct atgacgcctg tcacatcctc tctgagaccg acccggtgaa acaggcgtcg
      721 tggctggcac tttccgcttg gaccctcaac gctttggaaa gtctgctcga cctgttgggg
      781 attgagattc ccgacaggat gtagtcatgg ccagcacgat gcgtcccgcc cgtcaggacg
      841 acgttcccga gatcgaggcc tggacccggg acacctttgc ctggggcgac tacgtgggcg
      901 aaggcttgcc gacatggatc gaagacccgt cgtcactggt ggtggtctgt gtcgatgaag
      961 ccgaccgacc catcgcgatg tcacgatccg agatgctctc tccgaccgag ggctggctga
     1021 gcgcggcacg cgtccatccc gatcatcgtc gagccggcct gggcgcagcg atgaacgact
     1081 tcggggtcga gtgggcccgg gagcgaggag ccaagatcgt gcggctggtc atcgaggagg
     1141 acaaccccgc cgcccaagct caggtggcca agctcggcta tcggaacacc tgtgtctggg
     1201 tacgaggcga ggccaacacc ccgcgtggcc cgctaccacg ggaacgggac cgtctccacc
     1261 ccgcttcgag cgccgacgcc gacgccggat ggctgctctg gctgggcagc gagctggccc
     1321 gggccggccg ggaactcaac ccgggacact ggcgctggcg caaggcgctc cgctccgacc
     1381 tcgaccagcc cctggccgag agcagcctgt atcagggcgg agccggatgg gtggtcgcag
     1441 ttcccgacgg agacacgctg gcggtggggt ggatggtcac cgaccccgcc gattgcccgg
     1501 ctctattgga aggcatcgcc gaagtcgccc gtcagatggg aatggccagg atcgagatct
     1561 tcatgccgga gacgccgtgg agtcgcgaag ccctggtccg agagggattc accacccgac
     1621 gggtgctgat ctacgtcaag agcctctaga aactcaggtg aagatcgcca tcgtggcgat
     1681 ggcccagacc acgaacagga ccgggacctc ggccttgtgc aaccactccg gtgcgtttgg
     1741 cgcccagcgg atcagtagcg agaggggcac cccgaccaga ggcatgatca ccatcccggc
     1801 tatcccgacc gcacgcaagc cctcggctcc cttgtgcaac ggctcgggaa gatcacccag
     1861 aaagcgctcg atgaacccgt agaggagcaa ggccccgaac ccgaccagaa acacggcgac
     1921 ggtccggccg atccccacct cccagagctg gaagccgacc agtttccgcc ctcgcccgag
     1981 tggtccttcc gaggcgaccc cgaggcagaa gaggccgacg atggcgagga ccagggccga
     2041 agcgagcatg ccaccggttg agagctcctg gaccacattg acgatctcga aacctgccaa
     2101 cagcaccgcg atcgacaggc caaccagcag actccccaga accatcagcc ccacatcgag
     2161 catcgttccg aatgccccgt aggcgaggat cgcgtaacgc ttgaccatgg cgaggggatc
     2221 ggtgcggctg gcgctctcgg tcaacgtgac tcctccaa
//
LOCUS       DBNHXU010000207         3097 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2248546, whole genome shotgun sequence.
ACCESSION   DBNHXU010000207 DBNHXU010000000
VERSION     DBNHXU010000207.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3097)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3097
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2248546"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2405
                     /locus_tag="AC161X_05235"
     CDS             <1..2405
                     /locus_tag="AC161X_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012422.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4988987.1"
                     /translation="CEPVGIPTAFSPLFDLLDDLPTELCEDIRTGSGRTAVYAGMLDL
                     LKGDRIVLVVEDAHWADEATLGLIRYLGRRIAPTRSLLIATYRPEDLDLAHPMHLVVA
                     DLGPTATRIELPALTPSGVETMTRGLDVDPKRVHEATLGNPFLVEEVIRNPDSKLPSN
                     ISNAVLARAANFSSEALETLYSVALSPDGVSLDILLALDPAAGTYVDLAVQRRLLVSS
                     DGHVTCRHDLIRHSLEAAVPPALRRELHRRVLEELESQVDEHPDLSRLAYHSLSAQDD
                     LKAVNYSLQAAESSARGGAHRQAAYHYSNALLFRGRMTDGQLRDTLLAAAHEHCLTNA
                     SGVACGYAAELVDIAQSELETAGARAWLSFYKSRDNDLDATHQIAGLAIEVLEDEPPS
                     EGLALALAARAWVAMVEGRHAEAIQDGKRAIAVARVAGSARVEVHAATTVGTSLSLLG
                     DITGRALIEEAIALGIDRGADEETARAINNTALSHIWLFDLSGARERNEELVEFTTAR
                     ELDAWYITAVANLADIDLAAGRWVDADRELAMVLGQTTCRQTEVEAQMIAATLEIRRG
                     APGAELKLESALDRARGTTDLEIVRGAAILALQAAWTGVIELSETTPLLQEAERLPGW
                     ADDIWGRAMVGFWSMRTGIALPEEDLPGAAGMEQTGNLAEAADAWDRAGYTTEATICR
                     ALVPGADLDEIFSSLAALGADGVARGLRQELQRRGFKSVPRGERKSTRANPGGLTARQ
                     AEVLALMQAGLSNAAIAENLFISEKTAGHHVSAILAKLNVSSRLQAVAVTSGKGRNRA
                     PV"
     gene            complement(2438..2821)
                     /locus_tag="AC161X_05240"
     CDS             complement(2438..2821)
                     /locus_tag="AC161X_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018116369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4988988.1"
                     /translation="MAEEMVNVRYMVHDVDVAVAFYTTHFGFALRSNMAPAFADVVRG
                     QLRLLLSGPTSSAGRPMPDGAIPSPGGWNRIHFIVDDIEAEVERLRADGVRFRNDIIR
                     GPGGKQVLVEDLSGNPIELFEPAGG"
BASE COUNT          570 a          995 c         1003 g          529 t
ORIGIN      
        1 cttgcgagcc ggtggggatc ccgaccgcct tctcccccct cttcgatctg ctcgacgatc
       61 tgccgacaga gctttgtgag gacataagga cgggctcagg gcggaccgcg gtatatgccg
      121 ggatgctgga cctgctcaag ggcgaccgga tcgtcctcgt cgtcgaggac gcccactggg
      181 ctgacgaagc aaccctgggg ctcatccgct acctggggag gcgtatcgct cccacccgaa
      241 gcctgctgat agccacttac cgacctgagg atctcgacct cgcccacccg atgcatctgg
      301 tggtcgccga cttggggccg acggcgacca ggatcgagtt gccggccctt accccctccg
      361 gtgtggagac gatgactcgc gggctcgatg tcgacccgaa gcgggtacac gaagccactc
      421 tgggaaatcc gttcctggtc gaggaggtca tccgcaaccc cgactcgaag cttccgtcga
      481 acatcagtaa cgccgtcctg gcccgtgccg ccaacttttc ctcggaagct ctggagaccc
      541 tctattcggt ggctttgtcc ccggatgggg tgtcgcttga catcctgctg gcgttggatc
      601 ccgcagccgg cacctatgta gaccttgcgg tgcagcggag gcttctggtc tcctcggacg
      661 gtcacgtgac ctgtcgtcac gatctgatcc gacacagcct ggaagcggca gtgccacctg
      721 ccttgagacg cgaactgcac cgccgggtgc tggaagagct ggaatcgcag gtggacgagc
      781 accccgacct ctcccggctt gcctatcaca gcctcagtgc acaggacgac ctgaaggctg
      841 tcaactactc cctgcaggcg gccgagagct cggctcgtgg aggggcccac cgtcaggcgg
      901 cgtaccacta ctccaatgcc ctcctcttcc gggggcggat gaccgacggc cagctgcgtg
      961 acacccttct cgcggccgcc cacgagcatt gtctgaccaa cgcctcaggc gtggcgtgcg
     1021 gatatgccgc ggagcttgtg gatatcgccc agagcgagct ggagacggcc ggggctcggg
     1081 catggctctc cttctacaag tcacgggaca acgatcttga tgccacccac cagatcgccg
     1141 ggctcgccat cgaagtcctc gaggatgagc cgccatccga ggggctcgcg ctggcgctcg
     1201 ccgctcgggc atgggtagcc atggtcgaag gccgtcacgc ggaggcaatc caggacggga
     1261 agcgggcgat cgctgtggcc cgggtcgccg gctcggcgcg agtggaggtc catgccgcca
     1321 ccacagtcgg cacatccctt tccttgctcg gtgacatcac cggccgagct ctcatcgagg
     1381 aagccatcgc tctcggcatc gaccgcggcg ccgatgagga gaccgcgagg gcgatcaaca
     1441 acactgcgct cagccacatc tggctcttcg atctgtccgg ggcccgggag cgcaacgagg
     1501 agcttgtgga gttcaccaca gctcgtgagc tcgatgcctg gtacatcaca gccgtggcca
     1561 atctggccga catcgacctc gccgcaggtc gctgggtgga cgccgatcga gaactagcga
     1621 tggtgctcgg ccagaccaca tgccgccaga ccgaagtcga agctcagatg attgcggcga
     1681 ccctcgagat tcgacgtggc gctccggggg cggaattgaa gctggagagc gccctcgacc
     1741 gggcgcgggg cactaccgat ctggagatcg tccggggcgc cgcgatcctc gctctccagg
     1801 cggcctggac tggggtcatc gaattgtcag agacgacgcc cctcctccag gaggcggaga
     1861 ggctgccggg ttgggcagat gacatctggg gccgcgccat ggtcgggttt tggtcaatgc
     1921 gcaccggcat cgccttaccc gaagaggacc tgccgggagc ggcaggtatg gaacaaaccg
     1981 gcaatcttgc cgaggccgct gatgcgtggg atcgcgccgg ctacacgaca gaggcgacga
     2041 tctgtcgggc actggtcccg ggagccgacc tggacgagat cttctcgtcg ctcgccgccc
     2101 tcggtgctga cggtgtcgcc cgtggcctga gacaggaact gcaaagacgc gggttcaaga
     2161 gcgtgccccg gggagagcgt aagtcgaccc gcgccaaccc gggcgggctc accgcccgtc
     2221 aggccgaagt cctggccctg atgcaggccg ggctgtccaa tgcagcgatt gccgagaacc
     2281 tgttcatttc ggagaagacg gcggggcatc acgtgtcggc gatcctggcc aagctcaatg
     2341 tgagcagccg tctccaagca gtggcggtga ccagcggcaa ggggcggaac cgcgcccccg
     2401 tctgacctcg gcgcctggcg aatgacagcg gactggctta tccgccagca ggctcgaaga
     2461 gctcgatcgg gtttccggag agatcctcga caaggacctg cttcccgcca ggccccctga
     2521 tgatgtcgtt cctgaaacgc accccatcag cccgcaagcg ctcgacctcc gcctcaatgt
     2581 catccacgat gaaatggatg cgattccacc caccagggct ggggatggcg ccatcgggca
     2641 tcggacggcc ggccgagctg gttggaccgc tgagcaacaa acgaagctga ccccgcacga
     2701 cgtcggcgaa ggccggagcc atgttggagc ggagagcgaa gccgaagtgt gtcgtgtaaa
     2761 aggcgaccgc gacgtccaca tcgtggacca tgtagcggac attcaccatc tcttcagcca
     2821 tgctgtggcc tccaatgagt tgtacgtagt ttcaggatgg aagcaagact aagaaactct
     2881 gggagagcga agtctcgcgg atggttggta gacgacctat cgccgctccg agactgagat
     2941 tcgggagtgt ccggtcgcta tcttggccgg accggagaga ctgaggcgtc ttgagctttg
     3001 ccttcaagga tgaagtcggc cgatcagtgg agtacctcgc gtctcatctg gcccaggaca
     3061 gcatccagca agacccctac tggccaaaat gggactc
//
LOCUS       DBNHXU010000208         1501 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5225026, whole genome shotgun sequence.
ACCESSION   DBNHXU010000208 DBNHXU010000000
VERSION     DBNHXU010000208.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1501)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1501
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5225026"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..732
                     /locus_tag="AC161X_05245"
     CDS             <1..732
                     /locus_tag="AC161X_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018627867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TSCPD domain-containing protein"
                     /protein_id="HIR4988989.1"
                     /translation="RNGYNQEARIGGHKVFLRTGEYDDGTLGEIFIDLAKEGATLKGI
                     LSCFAIAVSKGLQYGVPLEEFVDTFTFQTFEPRGMVEGHPNIKMANSIVDYVFRALGV
                     EYLERDELAQVPPNRDMELAGPIKGLAAEAGTPLELFDAAAEVDVEVVQRAAEFVDAE
                     PDSPVVPLPSGGTDAGARSERVEGGLATQVETKVVTGGAAAMVDSALKENMGDAPVCS
                     NCGHMTIRNGSCYVCLTCGDTTGCS"
     gene            complement(767..>1501)
                     /locus_tag="AC161X_05250"
     CDS             complement(767..>1501)
                     /locus_tag="AC161X_05250"
                     /inference="COORDINATES: protein motif:HMM:NF014741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR4988990.1"
                     /translation="RRLLDYWRSDVDLPGVRLEIEDQLRRRHFDVTHKPNGMVAGEFA
                     LSQIEGESLLTSLDALMPPPEASDSRTTSQRRADSLGDLSRSFLEGSESPIVGGERPH
                     INVHVDIPALKGLGGGLHETEDGFVLDPESVRELACDASVSRIVFGQGSEVLDVGRRT
                     RIIPAGLRRAVVARDRHCVESGCRHSARWCDVHHLVSWADGGETVIDNLCLLCRYHHT
                     QLHLGLLTLEDLDIRGPALLASRRWT"
BASE COUNT          294 a          469 c          487 g          251 t
ORIGIN      
        1 cgcaatggct acaaccagga agcgaggatc ggcgggcaca aggtgttcct tcgcaccggc
       61 gagtacgacg acgggacctt gggcgagatc ttcatcgacc tggccaagga aggggcaacc
      121 ctgaagggca tcctctcctg cttcgcgatc gccgtctcca agggactcca atacggggtg
      181 ccgctggagg agttcgtcga caccttcacc ttccagacct tcgagccgag gggcatggtc
      241 gaaggccatc ccaacatcaa gatggccaac tcgatcgtgg actatgtgtt ccgggccctc
      301 ggcgttgagt acctggagcg ggatgagttg gcccaggtgc cgccgaatcg tgacatggag
      361 ttggccggcc cgatcaaggg cctggccgcc gaggcaggaa ctccgctgga gcttttcgac
      421 gccgccgccg aggtcgatgt ggaggtggtg cagcgagcag ccgagttcgt cgacgccgag
      481 cccgactctc cggttgtccc cctgcccagc ggggggaccg acgccggcgc gcgaagcgag
      541 cgggtcgagg ggggcttggc cacccaggtc gaaaccaagg tcgtcaccgg cggcgcagcc
      601 gccatggtcg actccgctct caaggagaac atgggtgacg ccccggtttg ctccaactgc
      661 ggtcacatga cgattcgcaa cggcagttgc tacgtatgcc tcacctgcgg tgacaccacc
      721 ggttgtagct gagaaaccac ctcaaaggat ctgggggcaa ccgcccctag gtccatcgac
      781 ggctagcaag aagcgccggc cctcggatgt cgaggtcttc gagggtcaac aggcccaggt
      841 gcagctgggt gtggtggtag cggcacagta gacagaggtt gtcgatgact gtctcgccgc
      901 cgtccgccca cgagacaagg tggtgaacat cacaccatct ggccgaatgc ctgcagccag
      961 attcgacaca atgacggtca cgggcgacca ccgctctgcg gagacccgcg ggaatgatcc
     1021 gggtcctgcg acctacgtcg agcacttcgg agccctgacc gaacacgatc cgggacaccg
     1081 aggcatcaca ggcaagctct ctgaccgact cgggatcgag cacgaacccg tcctcggtct
     1141 catgcagccc gccccccagg cccttcagag ccgggatatc gacatggacg ttgatatgtg
     1201 ggcgctcgcc accgacgatc ggtgactccg atccttctag aaagcttcga gacagatcac
     1261 ccaaggagtc ggcccgacgc tgactggtgg tccggctgtc gctcgcttcg ggaggcggca
     1321 tgagggcatc gagagacgtg agcagtgact cgccttcgat ctgggacaga gcgaactcac
     1381 cggccaccat cccgttgggc ttatgggtca catcgaagtg acgtcgtcgg agttgatctt
     1441 caatttcaag tcggacgccg gggaggtcga cgtcgctgcg ccaatagtcg agcagccgcc
     1501 g
//
LOCUS       DBNHXU010000209         2769 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4629746, whole genome shotgun sequence.
ACCESSION   DBNHXU010000209 DBNHXU010000000
VERSION     DBNHXU010000209.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4629746"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..1420)
                     /locus_tag="AC161X_05255"
     CDS             complement(23..1420)
                     /locus_tag="AC161X_05255"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4988991.1"
                     /translation="MNTIYEQLQKLPYFADLPQRLLLRVCTASEQLHASPGEVIIEEG
                     SMSDGLYVVTDGELEVTKMSAGRSISLARVTAGAVLGEISLLDDVPRIATVTALTEVD
                     YIKVPSDTVTELLDNPQMVLRMLRTVTSRLRETEGLVRSEERMAGLGRMAAGLMHEIN
                     NPMAAVRHGTNSGRAIIEDLGDTARRLSKKGVVLPEETPAGSLTGEMSALARADAEDA
                     MGEWLSGLGISDGWELAPALLDAGWSEETLSKLAPDVDPETRADLLRWLGLRLLGIQV
                     ANEVKIGADRVSELIQAVKEFSYLDQGSIQTVNLKTSIDNALVLLKHRLKGGVKVIKE
                     YDPDLPEIEAFGAELSQVWINLIDNAIFAMDGEGELTITATRDGDGVLITIGDTGTGI
                     PEDVASRVFDPFFTTKEPGVGTGLGLATVHSIIVRHGGKLDFVTGPTGTTFRARLSPK
                     LPDAARGSEGGAGQS"
     gene            complement(1417..>2769)
                     /locus_tag="AC161X_05260"
     CDS             complement(1417..>2769)
                     /locus_tag="AC161X_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR4988992.1"
                     /translation="AINEVSLDHYIMKPWDPPEEKLYPILDDLLEDWWDHFTPRFEGI
                     SVIGNRWGRATHDVRAFLARNQVPYRSLDLGTDNEAEAVLADIGGDQTLLPVVVMPDG
                     AVLVQPTTADVANHIGLKTQPTEQSYDLVIVGAGPAGLAAAVYGASEGLATLLVESEA
                     PGGQAGQSARIENYLGFPKGLSGSDLSRRAVTQATRFGAEILTPAEVVSVTRHDPFRT
                     VGLHDGTEVSTKALLITTGVSYTKLDAPGVEDLTGAGVYYGTSRIEGAAHRDEPMFII
                     GAGNSAGQAAMFLSRFGSSVTVMVRGESLAEFMSQYLIDAITAEENIFVRYQSQLVEA
                     RGDTHLEELVLKNLATEETEVVPAGALFIFIGQSARTDWVGDDVDRDDRGFILSGSDC
                     GPHPKGWNLQRSPLPFETSVPGIFVAGDVRHGSVKRVASSTGEGAMAVRFIHEHLADL
                     "
BASE COUNT          470 a          884 c          885 g          530 t
ORIGIN      
        1 tatcgggtga agctctgacc gatcagctct gaccggcgcc gccctcggag ccgcgggctg
       61 cgtccgggag cttgggcgag aggcgagctc ggaacgttgt acccgttggt ccggtgacga
      121 aatcgagctt tccgccatgc cggacgatga tcgaatgcac ggttgccagg ccgaggccgg
      181 tgccaactcc gggctccttg gttgtgaaga acgggtcgaa cacccgggag gcgacatcct
      241 cggggatccc ggtcccggtg tcaccgattg tgatcagcac tccgtccccg tccctggtcg
      301 cggtaatggt caactccccc tcaccatcca tcgcgaagat cgcgttgtcg atgaggttga
      361 tccacacctg gctcagctcc gcgccaaagg cctcgatctc gggaaggtct gggtcgtatt
      421 ctttgatgac cttgaccccg cctttcaagc ggtgtttcag cagtacgaga gcgttatcga
      481 tggaggtctt gagattgacg gtctggatcg atccctggtc gagataggag aactccttga
      541 ccgcctgaat cagctccgac acccggtcgg ccccgatctt gacttcgttg gctacctgga
      601 tgccaagcag acggaggccg agccagcgaa gcaggtcggc ccgggtctcg gggtcgacgt
      661 cgggagcgag cttggacagt gtctcctcgc tccaaccggc gtcgagcagc gcgggggcca
      721 attcccagcc gtcgctgatc ccgagcccgg acagccactc acccattgca tcctcagcgt
      781 ccgccctagc gagggcactc atctctccgg tcagggaacc cgccggtgtc tcctccggaa
      841 ggacgacgcc tttcttggaa agacgtcgcg ccgtgtctcc caggtcctcg atgattgccc
      901 ggcccgagtt ggtcccgtgc cttaccgccg ccatcggatt gttgatctcg tgcatcagac
      961 cggccgccat ccggcccaat ccggccatgc gctcctcgct cctcaccaga ccttcggtct
     1021 ccctcagcct cgaggtgacg gtgcgcaaca tcctcaggac catttgtggg ttgtccagca
     1081 actcggtgac ggtgtccgag ggcaccttga tgtaatccac ctcggtcaga gcggtgaccg
     1141 tggcgattct gggaacgtcg tccaagagag agatctcgcc gagaactgca cccgcggtga
     1201 ctctcgccag tgagatcgat cggccggcgc tcatcttggt gacctcgagc tcgccgtcgg
     1261 tgaccacgta gaggccatcg ctcatcgagc cctcttcgat aatcacctcg ccggggctgg
     1321 cgtgaagctg ctcggaggcg gtgcacaccc gaagcaagag gcgctggggg agatctgcga
     1381 agtaggggag cttctggagc tgttcgtaga tcgtgttcac aggtcggcca ggtgctcgtg
     1441 aatgaagcgg accgccatcg ccccctcacc agtcgacgaa gcgacacgct tgactgatcc
     1501 gtggcgcacg tcgccggcca cgaagatccc cgggaccgaa gtctcgaagg gcagtgggga
     1561 gcgctgcaga ttccatcctt tgggatgggg accacagtcc gaaccggaga ggatgaagcc
     1621 tcgatcgtcg cggtcgacgt catccccgac ccagtcggtt ctcgccgact ggccgatgaa
     1681 gatgaacaac gccccggccg ggactacctc tgtctcctcg gtggcgaggt tcttgagcac
     1741 cagctcttcg aggtgggtgt cgcctcgggc ttcaacgagc tggctctggt agcggacgaa
     1801 gatgttctcc tcggccgtta tggcgtcgat cagatactgg gacatgaact cggcgagcga
     1861 ctctccccga accatcactg tcacgctgga tccgaatcgt gagaggaaca tggccgcctg
     1921 gcccgcggag ttcccggctc caatgatgaa catcggctcg tcccggtgtg cggccccctc
     1981 gatgcgggac gtcccgtagt agacacccgc gccggtgagg tcctcgactc ctggggcgtc
     2041 gagcttggtg tacgagacac cggtggtgat gagcagtgct ttggtgctca cctcggtgcc
     2101 gtcgtgaagg cccaccgtcc ggaacgggtc gtgcctggtg accgacacca cttcggcagg
     2161 ggtgaggatc tcggctccga accgggtggc ctgggtgacg gcccgtctcg ataggtcact
     2221 gccgctcagg cccttgggaa acccgagata gttctcgatt cgggcgctct ggccagcctg
     2281 gccccccgga gcctcacttt cgacgagaag ggttgccagg ccctccgatg ccccgtacac
     2341 ggcagcggcg aggcccgcag gcccggctcc cacgatcacg aggtcgtacg actgctcggt
     2401 gggctgggtc ttgagaccga tgtggttggc gacatcggcc gtggtcggct gcaccagcac
     2461 cgccccgtcg ggcatcacca ccaccggaag cagcgtctga tcacccccga tgtcggcgag
     2521 cacggcctct gcttcgttgt cagtcccgag gtcgagcgag cggtagggga cctggttgcg
     2581 tgcgaggaac gctctgacat cgtgggttgc ccgaccccat cgattcccga tcacgctgat
     2641 gccctcgaaa cgtggggtga agtgatccca ccagtcctcc aggaggtcgt cgaggatcgg
     2701 atagagcttt tcctcggggg gatcccaggg cttcatgatg taatggtcga gggacacctc
     2761 gttgatggc
//
LOCUS       DBNHXU010000210         2878 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1060078, whole genome shotgun sequence.
ACCESSION   DBNHXU010000210 DBNHXU010000000
VERSION     DBNHXU010000210.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2878)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2878
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1060078"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="AC161X_05265"
     CDS             complement(<1..486)
                     /locus_tag="AC161X_05265"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4988993.1"
                     /translation="MTSPSRLAMGLFRPIARNYERWASILSMGQDGRWRRRLVTSLEM
                     PEGSLILDVAAGTGSITRSLQAAGYRVISLDQSDEMLGQLDGPGSRLLATAESLPIRP
                     GAVDGLTAGYLLRYVEDLHAATAEMARVVRPGGMVAVLEFGRPDGVWGPLWWLYTRIA
                     LP"
     gene            complement(535..915)
                     /locus_tag="AC161X_05270"
     CDS             complement(535..915)
                     /locus_tag="AC161X_05270"
                     /inference="COORDINATES: protein motif:HMM:NF024864.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HIR4988994.1"
                     /translation="MRRAVAPVAALLFAVTACQGPTGSSAEVGVYEFQIEPSISTFDA
                     GTITLDVQNEGEYGHTLVITDAGGHVLSAGGVIAPGEAEEVSIDLAPGQYSFTCRIVA
                     QDDQGNVIDHYEQGMSADVVVRAG"
     gene            complement(912..2390)
                     /locus_tag="AC161X_05275"
     CDS             complement(912..2390)
                     /locus_tag="AC161X_05275"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4988995.1"
                     /translation="MATRAPKGTRPSRWWIALAAVFATLSVAAVVAAVVHQHDAGRPI
                     GEGQLFLADAQLGSAQINEAVDSGVKPEEAVRHLRNELGIEGVALVDSTGTITTATSD
                     PLVGTKVDNPVLAYGLESGRFAAVAAPIDQDLRIDGVHEWRSGDALYLALLPGEEGRP
                     SLLLFYDISELLARRAGALGIQATTVQLAAVAMLFAIAAMAVLIGRYHSVQRYREMAL
                     EAEFLRQNSESLQVHNAELEVARHNAEAALTLAEEKNRIRAEFVLMINHELRTPLTSV
                     VTGAELLLMTEPDAADRYEILEALVHDGRRLQTMIDQLLAVARVENRGLSFAPQPVKF
                     AKVCDAVALSHSRVISSHGPDCHGPDHDPTVMTDLQTLRNLASSLADNAFTHGARNVR
                     IHCSTNEVHEPHFVVGTVPESAVYLSISDDGPGIRPEFLPRAFEKFEKDSRSSGTGLG
                     LYMARLMAEAMGASLLVWTSSEGTTFTIALPLTRVAAKLGAA"
     gene            complement(2436..>2878)
                     /locus_tag="AC161X_05280"
     CDS             complement(2436..>2878)
                     /locus_tag="AC161X_05280"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR4988996.1"
                     /translation="SIFGASYGGYLVLASLVHPENPFACGVAKFGDFNLLTSWHQTDR
                     IGRDDAIRLYGHPEENPDAYRAGSPIFGVPNLTTPILIAQGEKDPRVHLKQAEELVEA
                     LESNGKPYEYVTYPTEAHGLLRREPQLHFYRRLGRFLDWHTRPT"
BASE COUNT          531 a          892 c          918 g          537 t
ORIGIN      
        1 gggcagggcg atcctcgtgt acagccacca caacgggccc cagaccccgt ccggccgtcc
       61 gaactcgagc actgcgacca tgcctccggg tcgcaccact cgggccatct cggcagttgc
      121 ggcgtgaagg tcttccacat agcggagcag gtatccggct gtgagtccgt ccacggctcc
      181 agggcgaatc ggcagagact cggccgtggc cagaagccgc gagcctggcc catccagctg
      241 gccgagcatc tcgtcgctct ggtccagtga gatcacccgg tagccggcgg cctggagaga
      301 tctggttatc gagcctgtcc ccgcggcaac gtcgaggatc agcgagcctt ctggcatctc
      361 gagtgaggtc acaagacggc ggcgccatcg cccgtcctga cccatgctca gaatcgaagc
      421 ccaccgctcg tagttgcggg ctatcggtcg aaacaggccc atggcgagcc gggagggcga
      481 ggtcacaagc cgaaggtacc gatgactggg agtctttgca gacccccctt gatctcagcc
      541 cgccctgacc accacgtctg cggacattcc ctgttcgtaa tggtcgatca cgttgccttg
      601 atcgtcctgg gccacgatgc ggcatgtgaa cgaatactgg ccaggggcga gatcgatgga
      661 gacctcctcc gcttcccccg gagcaatgac ccctccggct gatagaacat ggcctccggc
      721 atcggtaatg accagggtgt gcccgtactc cccctcattc tgaacatcga gggtgatggt
      781 cccggcgtcg aaggtgctga tcgaaggctc gatctggaac tcgtagaccc caacctcggc
      841 cgaggaaccg gttggcccct ggcaggcggt gaccgcgaag aggagcgcag cgacgggggc
      901 aactgccctt ctcatgccgc tccgagcttg gccgcgaccc tggtgagagg cagggcaatg
      961 gtgaaggtgg ttccctcgga agaggtccaa accaggagcg aggcccccat cgcctctgcc
     1021 atcaggcggg ccatgtagag ccccaatccg gtgcccgagc tgcgactgtc cttttcgaac
     1081 ttctcgaagg ccctgggcag gaattcgggt cgaatcccag gcccgtcgtc gctgatcgac
     1141 agataaacag ctgactccgg aacggtcccg acgacgaaat ggggttcatg cacctcattg
     1201 gtggagcagt gaatcctcac gttgcgggct ccgtgggtga aggcgttgtc ggctagcgag
     1261 gacgccaggt ttcgcaacgt ctggagatcg gtcatcacag tcgggtcgtg gtcaggcccg
     1321 tggcagtcgg ggccgtggct cgagatcact cgggagtggg agagggcgac cgcatcgcac
     1381 accttggcga atttcaccgg ttggggagca aaggagagac ctcgattctc gactcgcgcc
     1441 accgccagca gttggtcgat catcgtctgt agtcgccgac cgtcgtggac cagggcctcg
     1501 aggatctcgt agcggtctgc tgcgtcgggt tcggtcatga gaagcaactc ggcgccggtc
     1561 acaacgctgg tcaatggggt cctcaattcg tgattgatca tgaggacgaa ctcggcgcgg
     1621 atccggttct tctcctctgc cagcgtcaac gctgcttcgg cgttgtgccg cgccacttcc
     1681 agctcggcat tgtggacttg cagtgattct gagttctggc gtaggaactc ggcttccaga
     1741 gccatctccc gatagcgctg caccgagtga tagcggccga tcagcactgc catggcggct
     1801 atggcgaaca gcattgcgac agctgccagt tggacggtcg tggcctgtat gccgagcgct
     1861 ccggcacgcc tcgccagcaa ttctgagatg tcgtagaaga ggaggagcga aggccggcct
     1921 tcttcgcctg gcaacagcgc caggtagaga gcatcgcccg atcgccactc gtggaccccg
     1981 tcgattctca ggtcctgatc gatgggtgcg gccactgcgg caaatctccc cgactcgagg
     2041 ccataggcca gcaccgggtt gtccaccttc gtcccgacca gtgggtcgga tgtggccgtg
     2101 gtgatggtgc cggtcgagtc gaccagcgcc actccttcaa tcccgagttc gttcctcagg
     2161 tggcgaaccg cctcttcggg cttgactccc gaatccacgg cttcgttgat ctgtgccgac
     2221 ccgagttgag cgtcagcgag aaagagttgg ccctcgccga tgggccggcc ggcatcgtgc
     2281 tgatgcacca cggcggcaac aacggcggcg acggacaagg tcgcaaacac cgccgccagc
     2341 gcgatccacc agcgagacgg tcgcgtgccc ttcggggcgc gggtcgccat aggtaaaaat
     2401 cggctgtttt ggccttctgc ttgaggaaac cagtctcagg tcggtctggt gtgccagtcg
     2461 agaaaacgcc cgagacgccg atagaagtgc agctgtggct cgcgacgcaa caatccgtgc
     2521 gcctcggtgg gataagtcac gtattcatac ggtttcccat tgctttcgag agcttccaca
     2581 agctcctccg cttgtttcag gtgcacccgc ggatccttct cgccctgggc gatgagaatc
     2641 ggcgtcgtca gatttggcac gccgaatatc ggggatccgg cccggtaggc gtccgggttc
     2701 tcctcaggat ggccataaag cctgatggcg tcgtcgcggc caatgcgatc cgtttggtgc
     2761 cacgaggtga gcaagttgaa gtctccgaat ttggcgaccc cgcacgcgaa agggttctcc
     2821 gggtgcacca aggaggcaag gaccaggtag ccgccgtaac tggcaccgaa gatggaaa
//
LOCUS       DBNHXU010000211         2120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4233587, whole genome shotgun sequence.
ACCESSION   DBNHXU010000211 DBNHXU010000000
VERSION     DBNHXU010000211.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4233587"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..635)
                     /locus_tag="AC161X_05285"
     CDS             complement(198..635)
                     /locus_tag="AC161X_05285"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR4988997.1"
                     /translation="MNSVQKTLMATGNKVASRLYVGSNGRLGGSARGVPVLVLTVPGR
                     KTGKPRSVPVAYFAHDGGFLVAATAGGSKSDPEWIKNLGASTRAQVQVGEKQYDVDVR
                     IVEGSERDQLWRDVVVERAPGFGKYEEKSGGRVIPIAILTPRS"
     gene            717..926
                     /locus_tag="AC161X_05290"
     CDS             717..926
                     /locus_tag="AC161X_05290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988998.1"
                     /translation="MDIRTPENGTGGPKCRECGKPARFFISKGLACGTHAYDYLVGDE
                     VDSGPLPAQIRSLSSKHQRSLLRRP"
     gene            997..>2120
                     /locus_tag="AC161X_05295"
     CDS             997..>2120
                     /locus_tag="AC161X_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4988999.1"
                     /translation="MRLLKFAALVAAGTALLLAQLSIRPGGDVLLALLAFAIYAAMGG
                     LIIFRHDGHLTGWLLSSLGLAILLADGIHWIPGLSEMFARWVSSWVWTTVFALFAALT
                     LTFPTGHGPQGGSRWARLGRMTLWALPVLIVASALTETLGGSESGASTQNPIGFIPDW
                     VGSASLFAVIAILIGGSISLVVKRRRARGIERAQLSWVVFGVTLLAAAVTLTFVYIAG
                     SIALGAGDPGDPAWIVVFLVMILFPASFGVAVLRYRLFDIDRIVSRTVGYTLVVGTLA
                     VIFAIIVTVLTSLLPAESDIAVAGSTLAVAALFTPLRRRIQDWVDRRFNRAQYDARRV
                     SDRFGNLVRDEVDMDRIVEGWVDVISETMQPTTIAVWVTN"
BASE COUNT          359 a          644 c          648 g          469 t
ORIGIN      
        1 gcaccaaata ccgctccggc caccaccagg gcgatcagaa ttcttttcat accacgtctt
       61 cctaatcagg ggaacgtgtc tttatcgcca cgaaaatggg ggtccttgag taggtcgctc
      121 gacctccgat ggctcagagg acacgggctg ttcggcccga gcggctggct gagcgagtcg
      181 gttcctcgct ctagaaatca ggaccgtggg gtgaggatgg cgatcggtat cacccgaccg
      241 ccggacttct cctcgtactt gccaaagcca ggcgcacgct cgaccaccac atcccgccag
      301 agctggtcac gttcagagcc ttcaacgatc ctgacgtcga cgtcgtactg cttctcaccc
      361 acttggacct gcgcccgggt cgatgccccg agattcttga tccactcagg atcggacttc
      421 gaaccgccgg ccgtggccgc gaccaagaat ccgccgtcgt gagcgaagta ggcaaccggg
      481 acgcttctcg gctttccggt cttgcgcccg gggaccgtca aaacgagtac gggtacgcct
      541 cgggcagagc ctcccaagcg accgttggag ccgacgtaga gccgggaggc cactttgttc
      601 ccggtggcca tcagcgtctt ttggacggaa ttcatcgatc ctccgagtgt ctagtcactt
      661 tgtgcgcgcc ctttctagtc ctcttgcgcg aaggccctta ttgccgatct ggaagggtgg
      721 acattcgcac acccgaaaac ggcactggcg ggccgaaatg ccgcgagtgt ggcaaaccgg
      781 ccaggttctt catctccaag ggcctcgcct gtgggaccca cgcctacgac tacctggtcg
      841 gcgacgaggt cgattctggc cctctgccgg cgcagatcag gtcgttgtcc agcaagcatc
      901 agcgaagcct gctgcgtcgt ccctgacctg ctggcatcgt ggcgtcgtgt gccacgagta
      961 acgatcccgt aacgggttcc ccaatagcat cgcaacatga ggctgttgaa gtttgccgcc
     1021 ctcgtggccg cgggcaccgc gttgctgcta gctcaactct ccatcaggcc cggcggcgac
     1081 gtcctgcttg ctttgcttgc tttcgcgatt tacgccgcaa tgggcggcct gatcatcttt
     1141 cgacacgatg gccatctcac aggctggctg ctgagctctc tggggttggc catcctcctc
     1201 gccgatggga tccactggat cccaggattg tcggagatgt tcgctcgatg ggtgtcgagt
     1261 tgggtttgga cgacggtttt tgccctcttt gctgcgctga ccctgacctt tccgaccgga
     1321 cacgggcctc aaggagggag tcgatgggct cgactcggcc ggatgacgtt gtgggcgctt
     1381 ccggtactga ttgtcgccag cgcgctcacc gagactcttg ggggctcaga gtcaggagct
     1441 tcgactcaga atccgatcgg tttcatcccc gactgggttg gatcagcgtc gttgtttgcg
     1501 gtgatcgcga tcctcattgg gggctcgatt tcgctcgtcg tgaaacggcg tcgagcccgc
     1561 gggatcgagc gcgctcagct cagctgggtg gtcttcgggg tcacccttct ggcagcagcg
     1621 gtaactctca cctttgtcta tatcgcggga tcgatcgctt taggcgccgg cgacccggga
     1681 gacccggcct ggatcgtcgt atttctggtg atgatcctgt tcccggcttc gttcggggtg
     1741 gcggtgcttc ggtatcgact cttcgatatc gatcgaatcg tgtcccggac agttgggtac
     1801 acgctggtgg tggggacgct ggcagtcata ttcgcaatca tcgtcacggt gttgacttcg
     1861 ctcctgcccg ccgaaagtga catcgcggtt gcaggatcga ctctggccgt ggcggcactc
     1921 ttcacgcccc tgcgtcgccg gatccaagac tgggtcgacc ggaggttcaa ccgagcccag
     1981 tatgacgccc gacgagtcag tgaccgcttc ggcaatctgg tcagagacga ggtagatatg
     2041 gaccgaattg tggaagggtg ggttgatgtg atctctgaga ccatgcagcc aaccacgatt
     2101 gcagtctggg tgaccaacga
//
LOCUS       DBNHXU010000212         2122 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4630473, whole genome shotgun sequence.
ACCESSION   DBNHXU010000212 DBNHXU010000000
VERSION     DBNHXU010000212.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2122)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2122
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4630473"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..835)
                     /locus_tag="AC161X_05300"
     CDS             complement(<1..835)
                     /locus_tag="AC161X_05300"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: protein motif:HMM:NF002320.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0002189 - ribose phosphate
                     diphosphokinase complex [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0006015 - 5-phosphoribose 1-diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009165 - nucleotide biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HIR4989000.1"
                     /translation="MDIVSRKRMMLFSGGANLPLAEEVAEVMGVSLGALERSRFANGE
                     IYVRPTESVRGADCFVMQSHCEPMNDNIMEQLITIDALTRASARRITAVMPFFGYSRQ
                     DKKVLPREPITARLISDLFMTAGADRLVSVDLHTGQLQGFINKPFDHLTALPIITDYL
                     SQRIEGPTTVLSPDAGGVKRAERYARFLDASVAFVYKRRDPASHNTSTALEMAGSVQG
                     RHVVIVDDIIDTGGTVCNAAEMVRAQGAVSVRIAATHGVFSEPSIDLLKNAAVEEVIV
                     TN"
     gene            complement(837..>2122)
                     /gene="glmU"
                     /locus_tag="AC161X_05305"
     CDS             complement(837..>2122)
                     /gene="glmU"
                     /locus_tag="AC161X_05305"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR01173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HIR4989001.1"
                     /translation="VLETVAGLDPETTVVVVGHRADEVERLLPDHVVSAVQEEQLGTG
                     HATSIGLNAIKDIDPGDTVVVLYGDLPLLTPDLVSGLAARGENVAARLVVVEFDNPTG
                     YGRVIHDENGLVTSIVEEKDTNEEQRALRDINAGVYSFRAGDLIDALARISNENAQGE
                     YYLTDVIGILADRGERIEPVRAVPEEVVGINSQDQLAEARRLLQARTNQRLMESGVWM
                     LDPERTYVDDTVTVEPGARIYPGVHLEGGTTVGAGAQVGPDVFAVDSSIGAGAMVWYA
                     VLRGAVVGDECEVGPYASLRPGSVLERGSKVGTFVETKNTTLGEKAKAPHLSYLGDAS
                     VGARSNIGAGTITCNYDGFEKHRTEIGQDVFIGSDSMLVAPLTIGDGAVIAAGSVITE
                     DVAEGALAIERNEQKEIPGYARRRAQKRAARKSED"
BASE COUNT          384 a          689 c          648 g          401 t
ORIGIN      
        1 tgttggtgac gatcacttct tccactgcgg cgttcttcag aaggtcgatc gacggttcgg
       61 agaagacccc gtgggtagca gcgatccgca cactgaccgc accctgagcc cggaccatct
      121 ccgcggcatt gcacacggtt ccgccggtat cgatgatgtc gtcgactatg acgacatggc
      181 gtccctggac gctcccggcc atttccaggg cagtcgaagt gttgtgcgac gccggatcac
      241 gtcgcttgta gacaaaggcc accgaggcgt cgaggaaccg ggcatatcgc tccgcccgtt
      301 tcacaccacc ggcatccggg gagagaaccg tggtcgggcc ctcgatccgt tggctgaggt
      361 agtcggtgat gatcggcagc gcggtcaggt ggtcgaacgg cttgttgatg aagccctgga
      421 gctggccggt atggaggtcg accgagacca agcgatcggc gccggcggtc atgaacaggt
      481 cactgatgag acgtgcggtg atcggctcac ggggaagaac ctttttgtcc tgacgcgagt
      541 agccaaagaa cggcatcact gcggtaatcc ggcgggccga cgcccgggtg agggcgtcga
      601 tggtgatcag ttgctccatg atgttgtcgt tcatgggctc gcaatggctc tgcatcacga
      661 agcagtccgc gccgcggacg ctctcggtgg gtcggacata gatctcgccg ttggcgaaac
      721 gggagcgttc cagagcgcca agggagaccc ccatgacctc ggcaacctct tcggccagtg
      781 gaaggtttgc ccccccactg aagagcatca tccgcttccg ggagacgatg tccaactcag
      841 tcctccgact tcctggctgc ccgcttctgg gcgcggcgcc gagcgtagcc ggggatctcc
      901 ttctgctcgt tgcgctcgat ggcgagggct ccttcggcca catcctctgt gatcaccgag
      961 ccggcagcta tcaccgcgcc gtcaccgatc gtcaacggag ccaccagcat cgagtcggat
     1021 ccgatgaaca catcctgtcc gatctcggtc cgatgcttct cgaatccgtc gtagttgcag
     1081 gtgatcgtcc ccgcgccgat attgctcctg gcccccacgc tggcgtctcc gaggtagctg
     1141 agatggggcg ccttcgcctt ctccccgagc gtggtgttct tggtctccac aaaggtcccg
     1201 accttggacc cccgctcgag gacgctgccg ggtcgaagcg acgcataggg cccgacctcg
     1261 cattcgtcac cgaccacggc gcctctgagc accgcgtacc aaaccatggc cccggcgccg
     1321 attgaagagt ccaccgcgaa cacgtcaggc ccgacctggg cacccgcgcc aacagtggtg
     1381 ccgccttcga ggtggacacc ggggtaaatc cttgccccag gctccacggt caccgtgtcg
     1441 tcgacatagg ttcgctcggg gtcgagcatc caaacacccg actccatcag cctctggttg
     1501 gtgcgagctt gaaggaggcg tctcgcctcg gccaactggt cttgagagtt gattccgacc
     1561 acctcctcag gaacggcccg gaccggttcg atgcgctcac cacggtcggc aagtatccca
     1621 atcacgtcgg tgaggtagta ctcgccttgt gcattctcgt tcgaaatcct cgccagggca
     1681 tcgatcaaat caccggcgcg aaaggagtac acgccggcat tgatgtcgcg aagcgcccgc
     1741 tgctcctcgt tggtgtcctt ctcctcgacg atcgaggtaa ccagcccgtt ctcatcgtga
     1801 atcacccggc cataaccagt gggattatcg aattcgacca ccaccagccg ggcggcgacg
     1861 ttctcgccac gggcagcgag gccagagacc agatcagggg tgaggagggg caggtcccca
     1921 tagaggacga ccaccgtgtc gccgggatcg atgtctttga tggcgttcaa cccgatgctg
     1981 gtggcatgac cggtgcccag ttgctcctcc tggacagccg aaaccacgtg atcggggagg
     2041 agccgttcga cttcgtcggc gcgatgaccg acgactacca ctgtcgtctc aggatcgagc
     2101 ccggccaccg tctccagaac cc
//
LOCUS       DBNHXU010000213         3550 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4630576, whole genome shotgun sequence.
ACCESSION   DBNHXU010000213 DBNHXU010000000
VERSION     DBNHXU010000213.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3550)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3550)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3550
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4630576"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..246)
                     /locus_tag="AC161X_05310"
     CDS             complement(<1..246)
                     /locus_tag="AC161X_05310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989002.1"
                     /translation="MSQQSDTSIGRFFLPGPTEVDPEVLAAQTHPMIGHRGSAIQELI
                     AEIEVGLKQVFLTDRPVVISTSSATGLMEAGIRNGVGK"
     gene            complement(274..567)
                     /locus_tag="AC161X_05315"
     CDS             complement(274..567)
                     /locus_tag="AC161X_05315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989003.1"
                     /translation="MSSARLGGMIALALVVACTQTSGTARGVVLSVEGTLQDVTAFTV
                     LVEGEEWSMVPVPDGDYEFALPHLREHRLSGQPILVGWELVDNVRYALSLADG"
     gene            complement(564..1373)
                     /locus_tag="AC161X_05320"
     CDS             complement(564..1373)
                     /locus_tag="AC161X_05320"
                     /inference="COORDINATES: protein motif:HMM:NF021214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase assembly protein"
                     /protein_id="HIR4989004.1"
                     /translation="MPLPPWHPHLDVWLLIWVLGFGYWYANQRLRPYVAPDAPPATKG
                     QRWQWYGALGLLFVVSGWPIHDIGETSLFTLHMVEHMVIALVVPPLLLRGCPRWLGDV
                     TLGNPMVARIVRPLAKPVPAFVIFNATFLLIHWPEMVAWMATSQPIHGFVHAWLFGAS
                     ILMWLPIFSPTAAIPRLAPPAQMLYLFLQSLLPTIPGSFLTFSSVPLYPIYGNAALAY
                     GLTAVADQTIAGVIMKIAGGILLWSTIAVIWFRWTRQEREWERLEASLRAG"
     gene            complement(1373..1681)
                     /locus_tag="AC161X_05325"
     CDS             complement(1373..1681)
                     /locus_tag="AC161X_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HIR4989005.1"
                     /translation="MTVEPHHPTPAQYWKIAALLAVLTAIEVAIYYVHLEFDLGNLNT
                     ISLLILATLKFVIVVAFFMHLRYEKTVLNRFFVGGFVLASCLYAVVLAALGALIIRGS
                     "
     gene            complement(1678..2301)
                     /locus_tag="AC161X_05330"
     CDS             complement(1678..2301)
                     /locus_tag="AC161X_05330"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012872065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HIR4989006.1"
                     /translation="MTETLHEAPMDHPEHESTGVENRKLGMWLFLSSEFLFFGALISN
                     YLLYSNRPGFPDPYPSQIYDIPFTSVSSFVLLMSSLTMVLAHNALSRQDMNGTRTWLF
                     ATAALGAVFLGGQVFEFTEFVVNENMTLSTNPAASAFYVLTGFHGTHVAIGVIMLLSL
                     WGISRRPGGLSEKHGMNLELVGLYWHFVDIIWIVIFTVVYLLSVPPA"
     gene            complement(2294..>3550)
                     /locus_tag="AC161X_05335"
     CDS             complement(2294..>3550)
                     /locus_tag="AC161X_05335"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit I"
                     /protein_id="HIR4989007.1"
                     /translation="RQFGSLFYAPVGGGDPILWQHLFWLFGHPEVYILVLPAMGMVSE
                     ILPTFSRKPLFGYSAVVFAGAAIGFLGFGVWAHHMFASGITPVAQAAFGLSTMTIAIP
                     TGIKIFNWLGTMWQGRIRMNTSMLFAIGFVAMFTIGGLSGVTHAVVPSDWQQTDTYYI
                     VAHFHYVLFGGAIFGLFGGLYYWFPKLTGRVMNERLGKTHFWLQFLGFNLTFGPMHWL
                     GLQGMVRRTWKYAPETGLQPWNLVVTIGGFIIAASIAIFIFNWWTSKRHGEPSGLDPW
                     DARTIEWMVPNPSPEYNFAVPPVVTSLDHFWQLKYDKGPDGRAVRKPDADEIVAKIEH
                     DQRNPVSPIHLPNPSYFPFFMAIAFPTIFYGIIYHTAMWGKALIFVGVLIGLIAVVGW
                     AIEPLEEPHVEHDDEGHTIEAAGAYD"
BASE COUNT          783 a         1044 c         1154 g          569 t
ORIGIN      
        1 tttccccacc ccgttgcgga tcccggcctc catcagcccg gtggccgacg acgtggaaat
       61 caccacgggc cggtcggtga gaaagacctg cttgagaccg acctcgatct cggcgatcag
      121 ctcctggatg gcagatccgc ggtggccgat catcggatgc gtctgggctg cgagcacctc
      181 gggatccacc tcggtgggcc cgggaagaaa gaaacgaccg atcgacgtgt ctgactgctg
      241 gctcaagaat gcgctccgtt ggttccgagc ccgctatccg tcggccaggc tcaatgcgta
      301 cctgacgtta tccaccaact cccaaccaac caatattggc tggccggaga ggcggtgctc
      361 acgcaggtgt ggcagcgcaa actcgtagtc accgtccggg accgggacca tgctccattc
      421 ctccccctcc acaagcacgg tgaaggccgt cacgtcctgc agagtcccct cgaccgaaag
      481 gaccaccccg cgagcggtgc ctgaggtctg ggtgcaggcg acgaccaggg cgagggcgat
      541 catcccgccc aatcgggctg agctcaaccg gctcgaagcg aggcttccag tcgctcccac
      601 tccctctcct gcctggtcca gcggaaccag atgacagcga tggtcgacca gagcaggatc
      661 ccgccggcga tcttcatgat gacgccggcg atggtctgat ccgcgaccgc agtgaggcca
      721 taggcgagtg cggcattccc gtagatcggg tagagcggga ccgacgaaaa cgtgaggaac
      781 gagccgggaa tcgtcggaag cagggactgg agaaagagat agagcatctg ggccggaggg
      841 gcaagccggg gaatggccgc agttggggag aagatcggca gccacatcaa tatcgacgct
      901 ccgaacagcc aggcgtgcac gaagccgtga atcggctggc tggtcgccat ccaggcaacc
      961 atctccggcc aatgaatgag gagaaacgtc gcattgaaga tcacgaaggc aggcaccggc
     1021 ttggccaggg gtcgcacgat ccgcgccacc atcggatttc cgagagtgac gtcccccagc
     1081 cagcgagggc atcctcggag cagtagaggc ggcaccacca aggcgatgac catgtgctcc
     1141 accatgtgca acgtgaagag agacgtctct ccaatgtcgt ggatcggcca ccccgacacc
     1201 acgaagagaa ggcccagtgc gccgtaccac tgccaacgtt gccccttggt cgccggcgga
     1261 gcatcgggcg cgacgtacgg tcgtaggcgc tggttggcgt accagtaacc gaagccgagg
     1321 acccagatca gcagccagac gtcgagatga gggtgccagg gaggcagagg catcaggatc
     1381 ccctgatgat cagggctccc aaggcggcaa gtaccacggc gtacagacac gaagccagga
     1441 cgaaaccgcc gacgaagaag cggttcaaca cggtcttctc gtatcggagg tgcatgaaga
     1501 aggcgaccac gatcacgaac ttgagggtcg ccagtatgag cagcgagatc gtgttgaggt
     1561 tcccgagatc gaactcgagg tggacgtagt agatcgccac ctcgatcgcg gtgagcacgg
     1621 cgagcagcgc cgcgatcttc cagtactgcg ccggagtcgg gtggtggggc tcgacggtca
     1681 tgctgggggc accgagagaa ggtagaccac ggtgaagatc acgatccaga tgatgtcgac
     1741 gaagtgccag tagaggccga ccagctcgag attcatcccg tgcttctcgc ttagcccacc
     1801 gggccgcctc gagatccccc agagggagag aagcatgatc accccgatgg cgacgtgggt
     1861 cccgtggaac ccggtgagca cgtagaaggc cgaggcagcc ggattggtcg agagcgtcat
     1921 gttctcgttg acgacgaatt ctgtgaactc gaacacctgg ccgccaagga acaccgcgcc
     1981 gagcgcggcg gtggcgaaca gccaggtcct ggtcccgttc atgtcctggc ggctcaaggc
     2041 gttgtgggcg agcaccatgg tcaacgagct catcaggagc acgaacgagc tgaccgaggt
     2101 gaaggggatg tcgtagatct gggaggggta ggggtcaggg aacccgggcc ggttggaata
     2161 gagcagatag ttcgaaatca gcgctccgaa gaagaggaac tccgaactga gaaagagcca
     2221 catcccgagt ttgcggttct cgacacccgt cgactcatgc tcgggatgat ccatcggggc
     2281 ctcgtggagg gtctcagtca tacgcgcctg ccgcctcgat ggtgtgtcct tcgtcgtcgt
     2341 gctcgacgtg gggctcctcc aatggctcga tggcccaacc gaccacggcg atcagtccaa
     2401 tgaggacccc gacgaagatc agcgctttgc cccacatcgc cgtgtgatag atgatcccgt
     2461 agaagatggt gggaaacgcg atggccatga agaacgggaa gtaagacgga ttgggaagat
     2521 ggatgggaga gaccggattg cgctgatcgt gctcgatctt ggccacgatc tcatcggcat
     2581 ccggcttgcg gacggctcgg ccgtcgggtc ccttgtcgta cttgagctgc cagaagtggt
     2641 cgagactggt gaccaccggc gggaccgcga aattgtactc cggtgagggg ttggggacca
     2701 tccactcgat cgtcctggca tcccaggggt cgaggcccga aggctcacca tggcgcttgc
     2761 tggtccacca gttgaagatg aagatcgcaa tcgaggcagc gatgatgaac ccgccgatcg
     2821 tcactacgag gttccagggc tggagaccgg tctcgggagc gtatttccag gttcgacgga
     2881 ccatgccctg caggccgagc cagtgcatcg gcccgaaagt caggttgaac ccgaggaact
     2941 ggagccagaa gtgggtcttg cccagtctct cgttcatcac ccggccggtg agtttgggga
     3001 accagtaata gagaccgccg aacagcccga agatggctcc gccgaacaac acgtagtgga
     3061 aatgggcaac gatgtagtag gtgtcggtct gctgccagtc cgaaggcacc accgcgtggg
     3121 tcaccccgga gaggccaccg atcgtgaaca tcgcgacgaa tccaatcgcg aacagcatcg
     3181 aggtgttcat ccgtattcga ccctgccaca tcgtcccgag ccagttgaag atcttgatcc
     3241 cggtcgggat cgcgatggtc atggtcgata gtccgaaggc tgcctgagca acaggcgtga
     3301 tccctgaggc gaacatgtga tgggcccaca cgccaaagcc gaggaaaccg atggctgcac
     3361 cggcgaacac caccgccgaa tacccgaaca ggggcttgcg agagaaggtg gggaggatct
     3421 cggagaccat gcccatggcc ggcaagacca ggatgtagac ctcgggatgg ccgaacagcc
     3481 aaaagaggtg ttgccacagg atcggatctc ccccgcctac gggcgcgtag aacaacgacc
     3541 cgaactgtct
//
LOCUS       DBNHXU010000214         2771 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3638006, whole genome shotgun sequence.
ACCESSION   DBNHXU010000214 DBNHXU010000000
VERSION     DBNHXU010000214.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2771)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3638006"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..562)
                     /locus_tag="AC161X_05340"
     CDS             complement(35..562)
                     /locus_tag="AC161X_05340"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017619175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HIR4989008.1"
                     /translation="MKAVELLTSLIRDIPDFPEPGVIFKDITPVLADGKALKVLVDAM
                     AEPFANSGITKVAGIEARGFTLATPVADRLGAGFIPVRKPGKLPFDTVREDYVLEYGT
                     DALEIHVDAAHEGERVLLVDDVIATGGTAAAATRLLRRVGADVVGISVFIELAFLNGV
                     DVLDGVPLNALVRYD"
     gene            complement(575..1540)
                     /gene="secF"
                     /locus_tag="AC161X_05345"
     CDS             complement(575..1540)
                     /gene="secF"
                     /locus_tag="AC161X_05345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00966.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HIR4989009.1"
                     /translation="MSGGFIRRMDRGETTIDFVGLRRRWFIISASFIIISLASLGIRQ
                     LNLGIEFVGGIGMQSPNPAGADIGEIRSALESVGVTSPTIQLLDDGAAVRISTEALET
                     SVANDAVEAISYVTGAERSEISTEAVGPSFGALVARRALLALGVFLGAVILFISWRLE
                     FKMAITAIVAVLHDLLMTIGVYSLTGFVVTPATVVAILTILGYSLYDGVVVFDKIEEL
                     VEIEDDKTYSEIVNLALNQVLARSLFTSLSSLLPVGSILLVGSVILGAPTLTEFALAL
                     FVGLAVGTYSSIFVAAPLLAVWKEKEPFWQERAQKLERRKRAGRK"
     gene            complement(1537..>2771)
                     /gene="secD"
                     /locus_tag="AC161X_05350"
     CDS             complement(1537..>2771)
                     /gene="secD"
                     /locus_tag="AC161X_05350"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0006886 - intracellular protein transport
                     [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HIR4989010.1"
                     /translation="MAQRIEEIGSVQEPDIQISGENTVIVQLPGVTDEQRAIEAVGQT
                     GVLTFRPVLGAVNGDVGPLAGTGTENIDDETGLTIVDDPESETWLLHEEAEFQEVLNL
                     GPAVLQGDEVADAVPVFDANSAVWAVSLDLTSEGGTAFAEMTANAAGFPIGDPQRQIA
                     IVLDGQVITAPAVNPTVSPDTGIAGGQAQITMGQGSDAQQEANDLAVVLRYGSLPVSF
                     EVSAVRKVSGTLGADSLQAGIIAGLLGLALVALVLVVAYRSLGLVAIVGLSVFATVLI
                     TLFGLLGQWVGLTLTLAGVTGLIVSMGISADTYIVYFERIKEKLRQGNTVQEATASGF
                     KIAFRTMLTANTVSLLGAVLLWALAVGAVKGFAISLGIGTVLDLIVALVFTRRAAGVM
                     AETRLGSGGWFSIEGAAE"
BASE COUNT          567 a          893 c          817 g          494 t
ORIGIN      
        1 atcgagcacc tcggccagcg tggccgtgcg cgactcagtc gtatcgtacg agagcattca
       61 gcggcacacc gtccaacaca tcgacgccat tcagaaacgc aagctcgatg aagacagaaa
      121 tgccgactac gtccgccccg acccgccgga gaaggcgggt ggccgctgca gcggttcccc
      181 cggtcgcgat cacgtcgtcg acgagaagga cccgctcccc ctcatgtgcg gcatcgacgt
      241 ggatctcgag agcatcggtg ccgtactcga ggacatagtc ctcacggacc gtgtcaaaag
      301 gcagcttgcc cggctttcga accggaatga atcctgcgcc gaggcgatcg gcaaccggcg
      361 tcgcaagggt gaaacctcgg gcctcgatgc cggccacttt ggtgatgccc gaattggcga
      421 acggctctgc catggcgtcc accaggacct tcaaggcctt cccgtcggcg aggaccgggg
      481 tgatgtcctt gaatatcact ccgggctcgg gaaagtcggg gatgtcacga atcaggctgg
      541 tgaggagttc cacggccttg attctacggg ttgatcattt ccttccagcc cgtttccgtc
      601 tttccagctt ctgagccctc tcctgccaga acggctcctt ctccttccac acggcgagaa
      661 gtggtgcggc gacgaagatc gacgagtagg taccgacggc caaaccgacg aacagcgcca
      721 atgcgaattc agtcaatgtc ggcgcaccga gaatcacact cccgaccaga aggatgcttc
      781 ccacgggcag caatgaggaa agagaagtaa acagagatcg ggccagcacc tggttcaggg
      841 caaggttcac gatctcgcta taggtcttgt cgtcctcgat ctccaccagc tcctcgatct
      901 tgtcgaacac gaccaccccg tcatacaacg agtacccgag gatggtgaga attgcgacca
      961 cggttgccgg tgtcaccacg aacccagtca acgaatagac gccaatcgtc atcagcaggt
     1021 cgtgtagcac cgccacgatg gcagtgattg ccatcttgaa ctccaggcgc cagctgatga
     1081 acaggatcac cgctccgagg aacacaccaa gtgccaacag ggcacgtcgg gccaccaggg
     1141 ccccgaagct cggcccaaca gcttcggttg agatctccga acgttcagca ccggtgacgt
     1201 acgaaattgc ctccacggca tcgttggcga ccgaagtctc cagggcttcg gtcgagatcc
     1261 gaaccgcagc cccgtcgtcg agaagctgga tcgtggggct ggtcaccccg accgattcca
     1321 gggcgctgcg aatctcgccg atgtcggcgc cggccgggtt cggggactgc atcccgatgc
     1381 caccgacgaa ctcgatcccg agattcagct gcctgatacc gaggctggcc aggctgatga
     1441 tgatgaacga cgctgagatg atgaaccatc tccggcgaag gccgacgaaa tcgatcgtgg
     1501 tctcgccacg gtccattctc ctgatgaatc cgccactcat tccgcagccc cttcgatact
     1561 gaaccagcca cccgacccga gacgggtttc agccatgact ccagccgctc tcctggtgaa
     1621 gaccagggcc acgatcaggt cgagcacggt gccgatcccc agcgagatgg caaagccttt
     1681 gactgctccg accgcgagag cccacaacag gaccgcaccc aagaggctga cggtgttggc
     1741 ggtgagcatt gtcctgaagg cgatcttgaa tcctgaggcg gtcgcctcct ggacggtgtt
     1801 cccttgcctc agcttctctt tgatgcgttc gaaatagacg atgtaggtat ccgctgagat
     1861 ccccatcgac acgatcaggc cggtcacgcc ggccagagtc agtgtgagcc caacccattg
     1921 ccccagtaac ccgaacagtg tgatcaggac ggttgcgaac acgctgagcc cgacgatcgc
     1981 cacaagtccc agcgaccgat aggcgacgac gaggacgagg gccacgagag ccagtccgag
     2041 gagaccggcg ataatcccgg cttgcagaga gtccgctccc agcgtgcccg agaccttgcg
     2101 cactgccgaa acctcgaagg agaccgggag tgacccgtaa cgcaagacga cggccagatc
     2161 attcgcctct tgttgggcat cggagccttg acccatcgtg atctgggctt gacctccggc
     2221 gatcccggta tcaggcgaca cggtcggatt gacggctggc gcggtgatca cctgtccgtc
     2281 gaggacgatc gctatctggc gttgcgggtc accgatgggg aacccggccg catttgctgt
     2341 catctcggca aacgctgtcc ctccttccga tgtcaggtcg aggctgaccg cccacaccgc
     2401 tgagttggcg tcgaacaccg ggaccgcgtc ggctacttcg tcaccttgaa gcacggccgg
     2461 ccccaggttc aggacctcct ggaactcggc ctcttcatgg aggagccagg tctcgctctc
     2521 cgggtcgtcc acgatggtca gacccgtctc gtcatcgatg ttctcggtcc ccgttccggc
     2581 gagggggcca acgtcgccgt tgaccgcgcc cagcacggga cggaaggtga ggacgccggt
     2641 ctgtccaacg gcctcgatgg cacgttgctc atcggtgacg ccggggagct gaacgatgac
     2701 cgtgttctcc cctgagatct ggatatccgg ctcctgaacg gagccgatct cctcgatccg
     2761 ttgggccatg a
//
LOCUS       DBNHXU010000215         3059 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4035308, whole genome shotgun sequence.
ACCESSION   DBNHXU010000215 DBNHXU010000000
VERSION     DBNHXU010000215.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3059)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3059
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4035308"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..427)
                     /gene="mobA"
                     /locus_tag="AC161X_05355"
     CDS             complement(<1..427)
                     /gene="mobA"
                     /locus_tag="AC161X_05355"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HIR4989011.1"
                     /translation="MSTLGAILAGGRSSRMGMDKAEVEVDGQTMLQRVASALQAFLPH
                     VVLLGADREGMECWPDDLPARGPLGGIASALSRMEDDHLLVVAVDQPFVRTETLARLG
                     DLKSEMTVVPVDALGVRQVTCAVYPKSIAEVAMEEAQAEG"
     gene            509..826
                     /locus_tag="AC161X_05360"
     CDS             509..826
                     /locus_tag="AC161X_05360"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989012.1"
                     /translation="MVLGTWLVVGPNTLLKILGMPITEEIWIRVVGVLVVILALYFYD
                     AAINEARHFFIATVLARMFSAAAFLAFALTGEPWQLAFFAMVDALGAFWTYVALRARQ
                     PIV"
     gene            complement(828..1949)
                     /locus_tag="AC161X_05365"
     CDS             complement(828..1949)
                     /locus_tag="AC161X_05365"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010615209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003872 - 6-phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008443 - phosphofructokinase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006002 - fructose 6-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HIR4989013.1"
                     /translation="MTRTIGILTAGGDSPGLNAAIRAIGKAAQRAYGMEVIGFKDGFQ
                     GLMLNRFARLDSDALSGILTRGGTVLGTSRARVDRMELEDGTTMDGRSVMREIVEHHQ
                     IEALVCLGGDGTMRNALRLEEAGIRTLGVPKTIDNDVTGTDVTFGFDTAMSIATDAID
                     RLHSTAHSHHRIMVVEIMGHDVGWLALGAGLAGGADVILIPEIPHDVEAVASSIKLRK
                     EAGSAFSIVAVAEGAYSREQAETQTAIVEELEAAIGSDRKRLKKSLKELQENRSKRTE
                     QLAATLETLTGLESRVTILGHVQRGGTPSAADRLLATRLGTACADYIDQGLRNVMVAA
                     RGEGTEPVPLEETAGITKFVPLDHPWLKSARSLGISLGD"
     gene            complement(2005..>3059)
                     /locus_tag="AC161X_05370"
     CDS             complement(2005..>3059)
                     /locus_tag="AC161X_05370"
                     /inference="COORDINATES: protein motif:HMM:NF012795.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AIR synthase related protein"
                     /protein_id="HIR4989014.1"
                     /translation="DAPLYDRPYSRPDWLDDLWEIPEPSSIEPVDALLQLLDDPAIGS
                     SAWVHEQYDHMLFLNTLRGPGGDGSLLRIRGTTKGLAVSTDGNGRLCLLDPRRGAARL
                     VYESALNVAMTGARPMAVVDNLNFGNPEKPEVMWQFRESVEGISEACEALGIPVVGGN
                     VSFYNETDGADIHPTPVVGLLGLADPIPTHLPGLSRANAGLEIWEVGPEATGNLAGSA
                     FQRVVEGAHRGRPTAPQPEAAKEVIELAAALAHQVLVLHDISTGGLAVTVAEICIVSG
                     VGAELWGQPDASLFSEDPHRFVVIMEPGTVTLPGGVARRIGTVGGDSLEIAGHKVGLD
                     LLTNTYREAIPRRMRG"
BASE COUNT          486 a         1010 c          998 g          565 t
ORIGIN      
        1 agccctcggc ttgtgcctcc tccatcgcca cttcggcaat cgactttgga tacacggcgc
       61 aggtcacctg ccgcactcca agcgcatcga ccggcaccac cgtcatctct gacttcaagt
      121 ctccgagccg ggccagtgtc tcggtgcgga caaagggctg gtctacggcg acgacgagca
      181 agtggtcgtc ttccatgcga gagagggcgc tcgcgatccc gccgaggggg cctcgggcgg
      241 gaaggtcatc gggccagcac tccatcccct cccggtcggc gcccagcagg actacgtggg
      301 gaaggaacgc ctggagcgcc gaggccacgc gctgcaacat cgtctgtccg tcgacttcga
      361 cctctgcctt gtccatcccc atccgggagc ttcggccgcc ggcgaggatc gcaccgagtg
      421 tgctcatcgg acgcaggcta ggttgaacgg gggaaggagg cccatgtcac ctgcggctcg
      481 aagtgtgtat gcgtggagcg tttacctgtt ggtactaggg acgtggctgg tggtgggacc
      541 gaacaccctg ctcaagatcc tcgggatgcc catcaccgaa gagatctgga ttcgggttgt
      601 gggagtcctc gtggtgatcc tcgctctgta tttctacgac gcggcgatca acgaggctcg
      661 tcacttcttc atcgcaacgg tgctggcccg catgttcagt gcggccgcat tcctcgcctt
      721 cgccctcacc ggcgagccct ggcagttggc gttctttgcg atggtcgacg cacttggggc
      781 gttctggacc tacgtggcgc tgcgagcccg gcaaccgatc gtctagatca gtctccgagg
      841 gagatcccga gactgcgcgc cgatttcaac caggggtgat cgaggggcac gaacttggtg
      901 atgccggcgg tctcctcgag gggcaccggc tcggtcccct cgccccttgc ggcaaccatc
      961 acgtttcgca agccttggtc gatgtagtcc gcgcatgccg tccccagccg cgtggcgagc
     1021 aagcggtcgg ccgcggacgg ggtcccgccc cgttgcacgt ggccaaggat ggtgactctc
     1081 gattcgagcc cggtgagggt ttccagagtc gctgccagct gctcggttct cttcgaccga
     1141 ttctcctgga gctccttcag ggatttcttg agccgcttcc ggtcgctccc gatcgcggct
     1201 tccagctcct cgacgatcgc cgtctgagtc tcggcttgct cacgtgaata ggcgccttcg
     1261 gcgacggcca cgatcgagaa ggcgctgccc gcctccttgc ggagcttgat cgaggaggca
     1321 accgcttcga catcgtgggg gatctcgggg atgagaatga cgtcggctcc gccggccagc
     1381 ccggccccga gagccagcca gcccacgtcg tgacccatta tctcgaccac cattatccgg
     1441 tggtgggagt gggccgttga gtgaaggcgg tcgatcgcat cggtggcgat gctcatcgcg
     1501 gtatcgaagc cgaaggtcac gtcggtcccg gtgacgtcgt tgtcgatggt cttcggcacc
     1561 ccgagggtgc ggatcccggc ctcttcgagt ctcagtgcgt tgcgcattgt cccgtcacca
     1621 cccagacaga ccagcgcctc gatctggtgg tgctcgacga tctcgcgcat cactgagcgc
     1681 ccgtccatcg tggtgccgtc ctcgagttcc atcctgtcga ctcgagcacg gctggttccg
     1741 agcaccgttc cgccccgggt caggatcccg gagagagcgt cagagtcgag acgcgcgaaa
     1801 cggttcagca tcaggccctg gaacccatct ttgaagccga tgacctccat cccgtatgcc
     1861 cgctgggctg ccttgccgat ggcccggatc gctgcgttga gcccggggct gtccccgccc
     1921 gcggtgagaa ttccaatggt tcgcgtcacc cggcgatggt atcgggtccc caggggcggc
     1981 tctgtcgagc tgagcgagtc gctgtcaccc ccgcatgcgg cggggaatcg cctcccggta
     2041 ggtattggtg agcaggtcca gcccgacttt gtgacccgca atctcgaggg agtcgcctcc
     2101 aacagtgcca atccgcctcg ccaccccgcc ggggagggtg acagttcccg gttccatgat
     2161 caccacgaag cgatgaggat cctccgagaa caggctggcg tcgggttggc cccagagctc
     2221 ggcacctact ccggagacga tgcagatctc ggccacggtc accgccaggc cacccgtgga
     2281 gatgtcgtga aggaccagaa cctgatgggc gagcgcggca gccaactcga tcacttcctt
     2341 cgccgcttcg ggctgcggtg cagtcggccg gccgcgatga gcaccctcga ccacccgctg
     2401 gaacgccgag ccggcgaggt tgcccgtcgc ctcgggcccc acttcccaga tctccaggcc
     2461 ggcattggct cggctcaaac cggggagatg ggtcgggatc gggtcggcga gaccgaggag
     2521 ccccaccacc ggagtggggt ggatgtctgc cccatcggtc tcgttgtaga acgagacatt
     2581 gccgccgacc accgggatgc ccagggcttc gcaggcttcc gagatcccct ccacgctttc
     2641 ccggaactgc cacatcacct ccggcttctc gggattgccg aaattcaggt tgtcgacgac
     2701 cgccatcggt cgggcaccgg tcattgccac attgagcgcg gactcgtaga ccaggcgggc
     2761 ggccccgcgc ctggggtcga gcaggcagag tcggccattg ccgtcggtgg acaccgcaag
     2821 gcccttggtc gtgccccgga tccggaggag cgagccgtcg ccccccgggc ctctcagcgt
     2881 gttgaggaac agcatgtggt cgtactgctc gtggacccag gccgaggaac cgatcgccgg
     2941 gtcgtcaaga agctggagca gggcatcaac cggttcgatc gatgacggct ccggaatctc
     3001 ccaaaggtcg tcgagccagt cggggcggct gtagggacgg tcgtagagcg gggcgtcgt
//
LOCUS       DBNHXU010000216         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2645986, whole genome shotgun sequence.
ACCESSION   DBNHXU010000216 DBNHXU010000000
VERSION     DBNHXU010000216.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2645986"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="AC161X_05375"
     CDS             <1..390
                     /locus_tag="AC161X_05375"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018873354.1"
                     /note="NH(3)-dependent; catalyzes the formation of
                     nicotinamide adenine dinucleotide (NAD) from nicotinic
                     acid adenine dinucleotide (NAAD) using either ammonia or
                     glutamine as the amide donor and ATP; ammonia-utilizing
                     enzymes include the ones from Bacillus and Escherichia
                     coli while glutamine-utilizing enzymes include the
                     Mycobacterial one; forms homodimers; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HIR4989015.1"
                     /translation="VGGYAVLKDVFKTLVYDLARWRNKDGTVIPQSSIDKPPSAELRP
                     DQKDSDSLPPYEVLDAILERYIEEDRSVGDIVADGYDPAVVARVCRMVDRNEYKRRQA
                     APGVKITTKAFGKDRRLPITNAYQPPL"
     gene            387..1193
                     /locus_tag="AC161X_05380"
     CDS             387..1193
                     /locus_tag="AC161X_05380"
                     /inference="COORDINATES: protein motif:HMM:NF013247.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HIR4989016.1"
                     /translation="MTSTILINGVASAEGVPVTDSSVLRGDGCFEVLKAYRGVPFALD
                     AHLDRLERSASALAIELPPRTEIATWIETVASECPECAVRVIVTRGSAIPNEPGEPMV
                     IVFAHPWAGSRGPARLLPVVAPWHAAGVPWDLAAAKVLSYAPNLSATRRAVGEGCDDA
                     LLLTVDELMLEGPTFCVAWVVDGVLETPSLDLGILDSITRRVVLDDAARLGIPLVEGR
                     WALQRLEEASEVMALSTIREVQPISMVGEVAFGEGPVTSELARVFAQRLS"
     gene            complement(1178..2320)
                     /locus_tag="AC161X_05385"
     CDS             complement(1178..2320)
                     /locus_tag="AC161X_05385"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HIR4989017.1"
                     /translation="MEGESTTTTPGGSSTSIPTPPKPGKCTPFEYQLVWPVLNRSQVR
                     DTFGEDRDHGARHHEGTDIHAPKLTPVVAVADGRVTKVTQEIGTENCCSIVIRHNDGW
                     QSVYVHLNNDHYGTDDGLGAGVRSDLVVGTKVEAGEIIGWVGDSGNAEETVDHLHFEL
                     RSPSGVAVDPVPSLRRARLKAEFPDADTTWPYLDNSGRAAQWVAGRLLSEGLFLPCDE
                     SGLNFCPDQVADPDFISDIARHLGGGEPPLLEGRFQEVPQVFEPLGDEQRVIDELLGC
                     PTPEPCLEFGVTETDLARVAAWALDRVPNAAIKNPEVVTGLDQLETYPTAEEAEALLR
                     ADGLIGTCRPPLDDEQLVTRESALISLSRWVVGPSIDCIALNQLRR"
     gene            2247..2585
                     /locus_tag="AC161X_05390"
     CDS             2247..2585
                     /locus_tag="AC161X_05390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989018.1"
                     /translation="MPGFGGVGIEVLEPPGVVVVDSPSIVVDVESGTVVEVVVVEDVV
                     VVEPGTVEGVVGRRGALSGGKRSLMVIGGHITAASTGAGDPPTTTKAAVNARTATMAA
                     PNLPGVMGAG"
     gene            2638..3144
                     /locus_tag="AC161X_05395"
     CDS             2638..3144
                     /locus_tag="AC161X_05395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989019.1"
                     /translation="MSLRKITLIPLAALAVFAMACDGGETALTTTSSLITGSGDGTST
                     TTVTVPEDQTSTTLVGQAVASYEIVAREPGTDGETVFVVIPPGTYTDVDLENFLGDLY
                     DAGTATYGAEIFDDAAAAAAYLKAEAERTPEEVALIEQHHFASLVGGTIIRFQGPFAE
                     SGEFVLGS"
BASE COUNT          548 a          986 c         1011 g          601 t
ORIGIN      
        1 gtgggtggtt atgcggtact gaaggacgtt ttcaagaccc tggtctacga cctggcccgc
       61 tggcgcaaca aggatgggac agtgattcct cagagcagca tcgacaagcc cccctcggct
      121 gagctgcgac ctgatcagaa agactccgat tctttgcctc cctatgaggt gctcgatgcg
      181 attctcgagc gatacatcga agaggaccgg agtgtcggcg acattgtcgc cgacggctat
      241 gacccggcag tggttgcccg ggtgtgccgc atggtcgatc gcaatgagta caagcggcga
      301 caggccgccc ccggggtgaa gatcacgacc aaggccttcg gcaaggatcg gcgtcttccc
      361 attaccaacg cctaccaacc accgctatga cctcgacgat cctcatcaat ggcgtggcct
      421 cggccgaagg agtcccggtg accgattcat cggtgttgcg gggcgacggg tgctttgagg
      481 ttctcaaggc ctatagggga gtcccgttcg ccctggacgc ccatctcgat cgcttggaac
      541 ggagcgcatc tgcgctggct atcgagctcc ctcctcgcac tgaaatcgca acgtggatcg
      601 agaccgtggc gtctgagtgc ccggaatgcg cggtccgggt gatagtcacc cgagggtcgg
      661 ccattcccaa cgagcccggc gagcccatgg tcatagtttt cgcccaccct tgggccggta
      721 gtcggggccc cgcccgactt ctcccggtag tggctccatg gcacgccgcg ggggtgccat
      781 gggacctggc ggcggccaag gtcctctcct acgcgccgaa cctgtcggcc acccggcggg
      841 cggtcggtga gggctgtgac gacgccctct tgctcacggt cgatgagttg atgctcgaag
      901 gcccgacttt ttgcgtggcc tgggtggtag acggcgttct ggagacaccg tccttggact
      961 tgggcatcct ggattcgatc acccgccggg tcgtgctcga cgatgcggct cgtctcggga
     1021 tcccactcgt ggaagggcga tgggccctgc aacgcctcga ggaggcgtcc gaggtgatgg
     1081 cgctgtccac gattcgagag gtccaaccga tttcgatggt cggggaagtg gcctttggcg
     1141 aaggcccggt caccagtgag ctggcgcggg tctttgctca gcgtctgagc tgattcaacg
     1201 caatgcaatc gatcgagggt ccgaccaccc atcgcgagag gctgatcaag gcagactccc
     1261 tcgtgaccag ctgctcatcg tctaggggcg gccggcatgt tccgatcagc ccatccgccc
     1321 ttagtagcgc ctcggcttcc tcggctgtcg ggtaggtctc gagctggtcc aacccggtca
     1381 cgacctcagg gttcttgatc gccgcgttgg gcacccgatc cagggcccag gcagccaccc
     1441 tggctagatc ggtctcggtc accccaaatt cgaggcatgg ctcgggggtc ggacagccca
     1501 agagctcgtc gatgaccctt tgctcgtcgc cgaggggctc gaagacctgt ggcacctcct
     1561 ggaagcgccc ctcgagcaag ggtggctcac ctccgccgag gtgacgggcg atgtcgctga
     1621 tgaagtccgg gtcggcgact tggtccgggc agaaattgag tcccgactcg tcgcagggaa
     1681 ggaacaggcc ctcggacagc agtcttccgg ccacccactg ggcggcgcgg cctgagttgt
     1741 ccaggtaggg ccaggttgtg tcggcgtcgg ggaactctgc cttcagccgg gcccgtcgca
     1801 ggctcggcac gggatcgacc gcgacacccg aggggctgcg caattcaaag tggagatgat
     1861 cgacggtttc ctcggcattc cccgagtctc caacccagcc gatgatctca ccggcctcca
     1921 ccttggtccc cacgacgaga tcggaccgaa ccccagcccc caggccgtcg tcggtcccgt
     1981 agtggtcgtt gttgagatgg acgtagaccg actgccatcc atcgttgtgt cggatcacaa
     2041 tcgagcagca gttctcggtc ccgatctctt gagtcacctt cgtgaccctg ccgtcggcca
     2101 ccgcgacaac cggagtcagc ttcggtgcat ggatgtcggt cccctcgtga tggcgggcgc
     2161 cgtgatcccg atcctcaccg aatgtgtctc ggacctgact ccgattcaga accggccaaa
     2221 cgagttgata ttcgaagggt gtgcacttgc ccggtttcgg aggcgtggga atcgaagtgc
     2281 tcgagccacc aggggtcgtc gtcgtcgact cgccttcgat tgttgtcgac gtcgaatccg
     2341 gaaccgtcgt cgaggtggtc gttgttgaag atgtggtcgt ggtcgaaccg gggaccgtcg
     2401 aaggcgtggt gggtcggcgt ggagccttgt cgggcggaaa gaggtcgttg atggtgattg
     2461 gcgggcacat cacggctgct tcaaccggag cgggagatcc tcccaccaca accaaggccg
     2521 cggtcaatgc caggacggcc acgatggcag ccccgaacct gccaggtgtc atgggtgcag
     2581 gctaacccga gtcagccttg ccgggaactg cgcgatccgc ggtaacgttc tccgggcatg
     2641 agtctccgaa aaatcacgct catcccgctg gccgcgctgg cagtgttcgc catggcctgc
     2701 gacggcggcg agacggccct gaccacaact tcgtcattga tcaccggttc cggcgacggc
     2761 acatccacga caacggtgac cgttcccgag gatcaaacca gcaccactct cgttggacag
     2821 gcggtcgcga gctacgagat tgttgcccgc gagcccggaa cggatgggga aacggtcttc
     2881 gttgtcatcc ctcccggcac ctatacggac gtcgatctcg agaacttcct cggcgacctc
     2941 tatgacgcgg gcactgccac ctatggagcg gagatcttcg atgatgccgc agccgcagcc
     3001 gcctacctca aagccgaggc tgaacggacg ccggaagagg tcgcgttgat cgagcagcat
     3061 cactttgcga gcctcgtcgg cgggacgatc attcgtttcc agggcccatt cgccgagtca
     3121 ggtgaattcg tcctcggcag ctgaca
//
LOCUS       DBNHXU010000217         3900 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5226989, whole genome shotgun sequence.
ACCESSION   DBNHXU010000217 DBNHXU010000000
VERSION     DBNHXU010000217.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3900)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3900
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5226989"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1012)
                     /locus_tag="AC161X_05400"
     CDS             complement(<1..1012)
                     /locus_tag="AC161X_05400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008201258.1"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HIR4989020.1"
                     /translation="MTDGLPERSTPANRVRFVTATTLFDGHDASINIFRRILQAAGAE
                     VIHLGHNRTGLDIVETAIEEDAHAIAVTSYQGGHMEFYRYVRDLLVERDAGHIRLFGG
                     GGGTILPTEGQELADYGVTRIYSPDDGRELGLQGMVADMIARSDYATGELGGDFDLSN
                     LTTANTGVVATLISAAENYPEKYADVFEQIESKAAASSGLVVGVTGTGGSGKSSLVDE
                     LVRRLLLDFADLRLAVISVDPSKRRSGGALLGDRIRMNSLPHPRAYMRSLATRQPNLS
                     LSPHVRQALDVVKAAGFDLVILETSGIGQSDTEIVDFSDLSMYVMTPEYGAATQLEKI
                     DML"
     gene            1159..1419
                     /locus_tag="AC161X_05405"
     CDS             1159..1419
                     /locus_tag="AC161X_05405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989021.1"
                     /translation="MTIEIDEGRFRITTGRRHMGSWPMREVKVERTSIYRFSFDIEGD
                     HLEFFPDDPSEFSDAVGAFIDLTDSKRGRFGLKARIQNASGS"
     gene            1447..2193
                     /locus_tag="AC161X_05410"
     CDS             1447..2193
                     /locus_tag="AC161X_05410"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012863.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HIR4989022.1"
                     /translation="MVRHLIKRGAIAGAAVIGIETAYAFLRPTPLLDEVDPSGTFGDP
                     SLPNLRVAVLGDSSVTAPGVAGPEEVWVSLICERLATDHHVVLESFAVGGSCAHDLIK
                     EQVGAAIDFEPDLIFVAVGGNDLIRGVSLQRFEVNLDHVVSDLSVTGATIVLSGVGDL
                     GTIPRLHPPLRQLVSHRSAGFDRIHRRVAARHGVMVIDQRSDRPDDWYSDPSLWSADL
                     FHVSAAGHRRWADVVWATLEPALKRVNEPR"
     gene            2180..2593
                     /locus_tag="AC161X_05415"
     CDS             2180..2593
                     /locus_tag="AC161X_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012894873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03668 family PPOX class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR4989023.1"
                     /translation="MNRADALARLEPSPIGHLATVRADGAPHLVPITFALDSDRIVHM
                     VDHKPKTTTRLQRLANIAANPRISLLVDHYDEDWTRLWWVRVDGMAQVVEDGTEWEAA
                     RLALALKYHQYQASPPSGPAVLITIVEVSGWAASG"
     gene            complement(2568..3113)
                     /locus_tag="AC161X_05420"
     CDS             complement(2568..3113)
                     /locus_tag="AC161X_05420"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HIR4989024.1"
                     /translation="MDKDEARRLARQSGPISPTQGSQVVDGLFSWLSTRLPGTIAAYL
                     AMADEVNVAELFDRLPGWRWVLPRVEDDGDLTFRDRDVPRETHHWGMIQPTDSGRVVP
                     VHEIDVFLVPGLAFDDFGGRLGRGGGYYDRVLAVRRSDSEALGVTLSSRVIPEVRVVD
                     HDQPVDWLAIEEGVIPTRPSR"
     gene            3133..3897
                     /locus_tag="AC161X_05425"
     CDS             3133..3897
                     /locus_tag="AC161X_05425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HIR4989025.1"
                     /translation="MATLAAMRWVIDVPRTLITVIAGVLATLIAVPLVIITAAINDVS
                     PFIEQVIRGWSRVWLICSGTKLEVVGSENIDPTRSYVVVANHLSALDIMACLLAVPIP
                     MRFLAKTELYKVPVLAQGMRAVGIVEVDRAARGSIHADVNRQGKALIQKGRSLIIYAE
                     GTRSRNGALRPFKKGAFTMALTSHLPVLPVSIHGSYEAWPPGQPWVHGGKIRVVIDPA
                     IETADMTQADGNQLRDRVRDVIAGRVSEMGGKVGSV"
BASE COUNT          743 a         1253 c         1167 g          737 t
ORIGIN      
        1 ccaacatgtc gattttctcg agttgagtgg ccgccccgta ctcgggagtc atcacgtaca
       61 tcgaaaggtc ggagaagtcg acgatctcgg tgtcggactg gccaatcccc gacgtctcca
      121 ggatcacaag gtcgaacccg gccgccttca ccacgtccaa cgcctggcgg acatgaggcg
      181 acagcgagag attgggctgg cgggtcgcca gagagcgcat ataggcccgg ggatggggca
      241 gcgagttcat ccggatccga tcccccaaca aggcgccccc ggagcgccgc ttcgaaggat
      301 ctaccgagat caccgccaaa cgcagatcag caaagtccaa cagcagtcgt cgcaccagtt
      361 cgtcgaccag actcgacttc cccgaccctc cggtgccggt cacccccacg accaatccgc
      421 tcgatgccgc cgctttcgac tcgatctgct cgaaaacgtc tgcgtacttc tccggatagt
      481 tctccgcagc cgagatcagg gtggcgacca cgccggtgtt cgcagttgtc aggttcgaaa
      541 ggtcgaagtc gccgcccagc tcacccgtcg cgtagtccga gcgggcaatc atgtcggcga
      601 ccatcccttg aaggccgagc tcccgaccgt cgtcggggga gtagatcctg gtcaccccgt
      661 agtcggccaa ttcctggcct tcagtgggga gaatcgtccc cccaccacca ccaaacaggc
      721 ggatgtgacc cgcgtcccgt tccaccaaca aatctctgac gtagcggtag aactccatgt
      781 ggccaccctg ataggaggtc accgcaatcg catgcgcgtc ctcctcgatc gcggtctcca
      841 cgatgtcaag accggtccgg ttgtgcccga gatggatcac ttccgctccc gcagcctgaa
      901 ggatccgcct gaagatgttt atcgacgcgt catgaccatc aaacagagtg gtcgcagtga
      961 caaatcgaac cctgttggcc ggggtcgaac gctctgggag gccgtcagtc atgctctcct
     1021 tcaaggacta gtccaaactg taccggtgga ttcggacgat tttccccgtt tcaacttgtc
     1081 gggaagacca ctagattgtg tcttgggatg gcttattact tagggcggct tcaaggctcc
     1141 aggctggagc caacacacgt gacgatcgaa atcgatgaag gtcggttccg gatcaccacc
     1201 gggcgtcggc acatgggttc atggccgatg cgcgaggtga aggtggagcg cacgtcgatc
     1261 tatcgattct ccttcgacat cgaaggcgat cacctggagt tcttcccgga cgatccctcg
     1321 gagttttccg acgccgtagg tgcattcatc gacctgacgg acagcaaacg cgggcgcttt
     1381 gggctcaagg cccggatcca gaacgcctcc ggcagctaac ccgcctcacc tgccaacatt
     1441 cggactgtgg ttcgtcatct gatcaagcgc ggcgcaatcg ccggagcagc cgtcatcggc
     1501 atcgaaacgg cctacgcctt tctccgcccc actcccctcc tggacgaggt cgacccatcc
     1561 ggcacttttg gcgacccctc gcttcccaat cttcgggttg ctgtgctcgg ggactcctcg
     1621 gtcaccgcac ctggagtcgc cgggcccgaa gaagtctggg tttcactgat ctgcgaacgc
     1681 ctcgccaccg accaccacgt ggtccttgag tcctttgccg tcggcggctc gtgcgcccac
     1741 gacctcatca aggaacaagt tggcgcggcg atcgacttcg agccggatct gatcttcgtt
     1801 gccgtaggag gcaatgacct gatccgcggg gtgtcgctgc agcgattcga agtgaatctc
     1861 gatcacgtgg tcagtgatct gtcggtgacc ggggccacca tcgtcctcag cggcgtcgga
     1921 gacctgggaa ccatcccccg tctccaccct ccgctccgcc aactcgtctc ccatcggtca
     1981 gcaggcttcg atcgaatcca ccgtcgggtc gctgcccgcc acggtgtcat ggtcatcgac
     2041 caacgctctg accgccctga cgactggtac tccgatcctt cgttgtggtc ggccgacctc
     2101 tttcacgtca gcgccgccgg gcaccgtcgg tgggctgacg tcgtctgggc cactctggag
     2161 ccagccctga agagggtgaa tgaaccgcgc tgacgcgctg gcgagactgg agccatctcc
     2221 gatcggccat ctcgccactg tcagggctga cggcgctccc catttggtgc cgatcacatt
     2281 cgcgttggac tcggatcgga tcgtccacat ggtcgaccac aagccaaaga cgaccacgcg
     2341 tttgcaaagg ttggccaata tcgcggctaa ccccaggatc agcctccttg tcgatcacta
     2401 cgacgaagac tggactcgcc tctggtgggt tcgggtcgat gggatggcac aggtcgtcga
     2461 agacggtaca gagtgggagg ctgctcgcct ggcgctggcc ttgaaatacc accagtacca
     2521 ggccagcccg ccttcgggac cggcggtgct gatcacgatc gtcgaggtca gcggctgggc
     2581 cgcgtcggga tgacgccctc ttcgatagcc agccagtcca ccggctggtc atggtccaca
     2641 acgcggacct caggaatcac ccgactggag agcgtgaccc cgagggcctc gctgtcggag
     2701 cggcgaaccg cgagtacccg gtcgtaatag ccaccacccc ggcccaatcg gccgccaaag
     2761 tcgtcgaagg cgagaccggg gaccaggaac acgtcgatct cgtggactgg cacgacgcgc
     2821 cctgagtcag tcggctggat catcccccaa tgatgggttt cccttggcac gtctcggtcc
     2881 cggaaggtga gatctccatc atcctccacg cggggcaaga cccatcgcca tccggggagt
     2941 cgatcgaaga gctcggcgac gttcacctca tccgccatgg cgagataggc ggcaatggtt
     3001 cccggaagtc tcgtggagag ccacgaaaac agcccgtcga ccacctgaga gccctgggtc
     3061 ggggaaattg ggcctgactg acgtgccagg cgacgtgcct cgtccttgtc cacaggatca
     3121 gggtaagggc caatggctac cctcgccgca atgcgctggg tgatcgatgt accgaggact
     3181 ctcatcactg tcatcgcagg cgttcttgcc acattgattg ccgtcccgtt ggtgatcatc
     3241 actgcggcga tcaacgatgt ctcacctttt atcgagcagg tgatccgggg ttggagtcgg
     3301 gtctggttga tctgctccgg aacaaagctc gaggtcgtgg ggtctgagaa catcgacccc
     3361 accaggtcct atgtagttgt ggctaaccat ctctcggcgc tggacatcat ggcctgtctc
     3421 ctcgcggtcc cgatcccgat gcggttcctc gccaagaccg agctctacaa ggtgccggtc
     3481 ctggcacagg ggatgcgagc ggtcgggatc gtggaggtcg accgggcggc ccgagggtcg
     3541 atccacgccg acgtgaaccg acagggaaag gccctaattc agaaagggcg ctcgctcatc
     3601 atctacgccg agggcaccag gtcccgtaac ggtgcgttac gaccattcaa gaagggcgcg
     3661 ttcacgatgg cgctcaccag ccaccttccg gtgcttccgg tttcgatcca cggttcctac
     3721 gaggcgtggc caccgggcca gccctgggtt catggaggga agatcagagt cgtcatcgac
     3781 ccggcgatcg aaaccgcgga tatgacccaa gcggatggca accaactgcg agatcgggtg
     3841 cgtgacgtga tcgccggaag ggtgtcagaa atgggcggga aagtgggctc ggtctagggc
//
LOCUS       DBNHXU010000218         7219 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3638712, whole genome shotgun sequence.
ACCESSION   DBNHXU010000218 DBNHXU010000000
VERSION     DBNHXU010000218.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7219)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7219)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7219
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3638712"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..190
                     /locus_tag="AC161X_05430"
     CDS             <1..190
                     /locus_tag="AC161X_05430"
                     /inference="COORDINATES: protein motif:HMM:NF014860.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NADPH:quinone reductase"
                     /protein_id="HIR4989026.1"
                     /translation="FVKLTSDPATGVVLGGTIVGHRASELIGIVALAVQARVTAETLV
                     ETLMVHPSMSEAISDAAE"
     gene            complement(240..1973)
                     /locus_tag="AC161X_05435"
     CDS             complement(240..1973)
                     /locus_tag="AC161X_05435"
                     /EC_number="6.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015442437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016421 - CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl/propionyl/methylcrotonyl-CoA carboxylase
                     subunit alpha"
                     /protein_id="HIR4989027.1"
                     /translation="MKSLLIANRGEIAVRVIRTAKEMGIRTIALYSELDRDALHVDLA
                     DEAWNAGPAPAAESYLNQQRILQVAHESGAEAIHPGYGFLSENAGFAKAVQDAGIIWV
                     GPPPEAIEGMGDKITSRRNAEKFGVPTVPGITEPVKSVEDARVIAEQFGYPVAIKAAH
                     GGGGKGLRAVHGPDDLADAFEGARREADAYFGNPEVYVEKYLEKPRHVEAQILFDSHG
                     KGVFLGERDCSVQRRHQKLIEETPSPGLTIKQRRALAKAALAAARSCGYVNAGTVEFL
                     VDRNGDFFFLEMNTRLQVEHTVTEMVTGLDLVEWQLRIARGEELTIDEVEPRGHAIEF
                     RINAEDPFNNYMPSPGQVVEWQEPAGPGVRVDSWVRQGTTVSQYYDNLMAKLVVWGPN
                     RASAINRGRRALQEFKIRGVATTIPAQLAVLDHPDFIKGEHHTRWMEESVILDPTSPD
                     APPALPEDEELAKRDITVEVGGRRFTVSYWAPEPATPGGGVRRRPPRLSAPGPGSASD
                     GVLTAPMQGTIVKVHVKAGASVKAGDAICVLEAMKMENEVTTPNAGEVVDLRVEPGDT
                     VSAGQVIAIVK"
     gene            complement(2070..3620)
                     /locus_tag="AC161X_05440"
     CDS             complement(2070..3620)
                     /locus_tag="AC161X_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007074621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA carboxylase subunit beta"
                     /protein_id="HIR4989028.1"
                     /translation="MLGTEARIEKLRELREEALHAGSQRAVARQHEQGKLTARERLDA
                     LLDKGSFQEIDQFVRHQASGFGVEDKRPLGDAVVTGHGTIDGRPVFVFAEDFTVFGGS
                     LSKVVADKICKILDMALDTGTPVVGLKDSGGARIQEGISSLDGYGRIFERNVRASGVI
                     PQISVIMGPCAGGAVYSPALTDFVIQVDQTSHMFITGPDVIKAVTGEEVTMEELGGAM
                     THASKSGVTHLVYPTGQDALEAVRYLMSFLPRNNMELPPFFTPTDRADRIDDELDSII
                     PDASSQPYDMIQVLETVLDGNEFFEIHEHWAKNIVIGFGRLDGHTVGVVANQPANLAG
                     TLDITASEKAARFVRFCDSFNIPIVTFVDVPGFLPGVDQEHNGIIRHGAKLLYAYCEA
                     TVPRLTVITRKAYGGAYLVMNARGIRADLVFAWPSAEIAVMGAQGAVNIIHRRELTAD
                     DGETKRAELIADYEETFNNPYVAAELGLVDEVIEPRETRYKLIRALEVLRTKREKMPP
                     KKHGNGPQ"
     gene            3661..4320
                     /locus_tag="AC161X_05445"
     CDS             3661..4320
                     /locus_tag="AC161X_05445"
                     /EC_number="6.3.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015084.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009374 - biotin binding [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HIR4989029.1"
                     /translation="MATPYWQMRREEVPSTQDLARSELATLPVVVVAAVQTAGRGRTG
                     AGWITAPQALAVSVAIPISQGEDRPLSLMAGVAARRALVGAVGLKWPNDLEIDDLKVG
                     GILVEQSDGIAVIGLGVNLWWPEPPEGMGAVGDEDPGPHRHLEVGALWAAELLALIGA
                     EGWPIDEYRESCGTLGREIAWEPEGSGKAIDVGGSGALLVQVGHDIVEIRSGAVRHVR
                     G"
     gene            complement(4317..5075)
                     /locus_tag="AC161X_05450"
     CDS             complement(4317..5075)
                     /locus_tag="AC161X_05450"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR4989030.1"
                     /translation="MTGYRLTMAVLAHEGIYPIMYGACPIPMGTGHRTGYLARPDAAG
                     KFPVVFVLPSLDGLTSFEKDLCRRLARNGLLALSVDFYRAGGDPLDRYNLLSDARAIA
                     DLDELHGYVVSDDIEWAVSDQIGIFGVDVGGRFGVISAATRPWVRSLALAYTPLTGDE
                     DREFQVADFLGHLPVPVLGLYGADDELIAASSVDEAQNRNQHGQWLLYPGVRHGFLDI
                     DAGEYDPSAAEDAMGRVIGFLKETLPEAEEEVLG"
     gene            5155..6423
                     /locus_tag="AC161X_05455"
     CDS             5155..6423
                     /locus_tag="AC161X_05455"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR4989031.1"
                     /translation="MTSSPSTPVSRRGLPRWLKVTILTLLVVANLAALGLLWALNTGQ
                     NLLSGADTDHEVVDVLTDPTGEALTFLVVGSDSREGLDDLEHFGNFAGARGDVVMLVR
                     VDPSTGEARMLSIPRDLWVDVPGQGMGKINSAYSYGGPSLMVRTIQENLGIEVNHYVE
                     VGFVGFQELVDDLGGIHISFPYAARDRSSGLDVEAGTDVLDGEMALAYARSRKYQEYQ
                     NGSWVSVDANDIGRTQRQQEVVRAILSELKSPASVADAGTMSSSLASHMTIDATLAEA
                     SIAGLAWDFRSLLTGGIEGTTLPVDGATKGGASVVIAREPEASEMIADFLGGTTQVSQ
                     EPLRVQVLNGNGVSGSAGKMSDALQESGFEVESIGNADSRDYEQTTIVVPSGSPNGGV
                     IASELGFGVVEFGDVDNAYDAIVIVGADAP"
     gene            6484..>7219
                     /locus_tag="AC161X_05460"
     CDS             6484..>7219
                     /locus_tag="AC161X_05460"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR4989032.1"
                     /translation="MDIAIIGAGYVGLVTGTGLAHLGHRVRIGEADGQRVERLRRGIV
                     PIFEDGLPALLAEAVDRELISFHTSNVEAAADSRLVFLCLPTPEAEDGRADLRYIHSV
                     IDELANEVDPDAIFVVKSTVPPGTVTSLRKRLADVGSQARIVSHPEFLREGRAVEDFL
                     APDRIVIGAWEEEDADAVAEIYSGVDTEVVRTDPTSAEMIKYANNAYLATRLTFVNTL
                     ANVCEAVGADIIDVVRGIGLDHRIGPH"
BASE COUNT         1273 a         2211 c         2330 g         1405 t
ORIGIN      
        1 gtttgtgaag ctgacctccg atcccgcaac tggagtcgtg ctcggcggca ccatcgtcgg
       61 gcatcgcgcc tcggagttga tcgggatcgt ggcgctggcg gttcaggcac gggtgactgc
      121 cgagactctg gtggagaccc tcatggtcca tccctcgatg tcggaagcca tctcggacgc
      181 tgcggaatag ttgtcctaag tcataagtcc caagtctcag gcatgtgact tgagacccct
      241 tacttgacga tggcgattac ctgtccggcg ctgaccgtgt ctccgggctc cacccgaagg
      301 tcgaccacct caccagcgtt gggcgtcgtg acttcgtttt ccatcttcat ggcttcgagg
      361 acgcagatcg cgtcccccgc cttgactgaa gcaccggcct tgacgtgaac tttgacgatc
      421 gtgccctgca tgggagcggt gagcactccg tctgaggcac ttcccgggcc gggagcggat
      481 agccgtggcg gccgtcgtcg cacgccacca ccgggggttg ccggctcagg cgcccagtaa
      541 gacaccgtga accgtcgccc accgacctca acggtgatat cccgctttgc aagctcctcg
      601 tcttcaggaa gggctggcgg ggcgtcgggc gaggtcgggt cgaggatcac ggactcttcc
      661 atccacctcg tgtgatgctc gcccttgatg aagtcggggt ggtcgagcac cgcgagttgt
      721 gccgggatgg tcgtggcgac gcctcgaatc ttgaattctt gcaaggcccg tcgccctcgg
      781 ttgatcgcac tcgcccggtt cggtccccag accaccagct tggccatcaa attgtcgtag
      841 tactgcgaaa ccgtggttcc ctgacgaacc catgagtcga ctctcacgcc gggaccggcg
      901 ggctcctgcc actccacgac ctgcccagga gagggcatgt agttgttgaa gggatcttcg
      961 gcattgatcc gaaactcgat ggcgtggccc cgaggctcga cttcgtcgat ggtcagctct
     1021 tcgcctctgg cgatcctgag ttgccactcg accagatcga gaccagtgac catctcagtc
     1081 acggtgtgct caacctggag ccgggtgttc atctcgagga agaagaagtc accgttgcga
     1141 tcgacgagga actcgactgt cccggcattg acataaccac aggaccgggc cgctgccagc
     1201 gcggccttgg ccagagcgcg gcgctgtttg atcgtaaggc cgggggatgg ggtctcctcg
     1261 atgagcttct gatgtcgccg ttgaacagag cagtcccgct ccccgaggaa cacacctttg
     1321 ccgtgggaat cgaagaggat ctgcgcctcg acgtgacgcg gcttctcgag atacttctcg
     1381 acgtacacct cggggtttcc gaagtaggcg tcagcctccc gtcgggcgcc ctcgaatgca
     1441 tcggcgaggt catcgggccc gtgcacggca cgcaggcctt tcccgcctcc gccatgggct
     1501 gccttgattg ccaccggata gccgaactgc tcagcgatga ccctggcgtc ttcgaccgac
     1561 ttgaccggct cggtgatccc gggaacggtg ggcacgccga atttctcggc gttgcgacgc
     1621 gaagtgatct tgtcgcccat gccttcaatg gcctcgggcg ggggaccgac ccagatgatc
     1681 ccggcgtcct gcacggcctt ggcaaagccg gcgttttcgg agagaaaccc atatccgggg
     1741 tgaatcgcct ctgcccctga ctcgtgggcc acctggagga tccgctgctg gttgagatag
     1801 ctctcggcgg cgggagccgg tcccgcgttc cacgcctcgt ctgcgagatc gacgtggagg
     1861 gcatctcggt ccaactcgct gtagagcgcg atggtcctga tacccatctc cttggcggtg
     1921 cggatcactc gaaccgcgat ctcgccacga ttggcaatca gcagcgactt catcgcttca
     1981 gtcccaagtc acaagtcaca agtcccacgc cagattgacc gcgtcttgtc tggagcttga
     2041 gacttgagac ctgagacttg agtctcctgt cactgcggac cgttcccgtg cttcttcgga
     2101 ggcatctttt cccgcttggt gcggagcact tcgagagccc ggatcagctt gtacctggtc
     2161 tcgcggggct cgatgacctc gtcgaccagg cccagttccg cagctacgta cggattgttg
     2221 aacgtttcct cgtagtcggc gatgagctct gctcgtttgg tctcaccgtc gtccgccgtc
     2281 agctcccggc ggtgaatgat gttgaccgca ccctgggcgc ccatgactgc gatctcggcc
     2341 gacggccagg cgaacaccag gtcggctcgg atgccacggg cattcatcac gaggtaggcg
     2401 ccgccatagg ccttgcgtgt gatgacggtg aggcggggca ccgtcgcttc gcagtaggcg
     2461 tagaggagtt tggcgccatg acggatgatg ccgttgtgct cttggtcgac accggggagg
     2521 aaacccggga catcgacgaa ggtgacgatc gggatgttga acgagtcaca gaatcggacg
     2581 aagcgagctg ccttctcgga ggcagttatg tcgagggtgc ccgcgaggtt ggccggctgg
     2641 ttggcaacga cgccaaccgt gtgcccgtcg agacgcccga agccgatcac gatgttcttc
     2701 gcccaatgct cgtggatttc gaaaaactcg tttccgtcca gaactgtctc caacacctga
     2761 atcatgtcgt acggctggct ggaggcatcc gggatgatcg aatcgagctc gtcatcgatc
     2821 cgatcggctc ggtcggtagg agtgaagaac gggggtagct ccatgttgtt gcgggggagg
     2881 aacgacatca gatagcgcac cgcctcgagc gcatcctgtc cggtcgggta aacgaggtgg
     2941 gtgacgccgg acttcgaggc gtgggtcatc gctcccccca actcttccat cgtcacttcc
     3001 tcgccggtca cggccttgat cacgtcggga ccggtgatga acatgtggct ggtctgatcg
     3061 acctggatca cgaagtcggt cagggcgggg gagtagaccg caccaccggc acacggtccc
     3121 atgatgaccg agatctgggg tatgaccccc gaagctctca cgtttcgctc gaagattcgg
     3181 ccgtacccgt ccagcgagga aatcccctct tgaatccggg cgccgccgga gtccttgaga
     3241 ccgaccacag gcgtgccggt gtccagcgcc atgtcgagga tcttgcaaat cttgtcggcg
     3301 accaccttgc tgaggcttcc cccaaagacc gtgaaatcct cggcgaagac aaagaccggc
     3361 ctgccatcga tggtgccgtg cccggtgacc actgcgtcgc cgagcggtcg cttgtcttcg
     3421 accccgaagc ccgaggcttg atgcctcacg aattggtcta tctcctggaa cgagcccttg
     3481 tccaggagcg cgtcgaggcg ttcgcgcgcg gtgagcttgc cttgctcgtg ctggcgagcc
     3541 acagctcgct gactccccgc gtgcagcgcc tcctcgcgga gttccctcag tttctcgatc
     3601 cgcgcctcgg tgcccaagat tctctccgta acctcacact tcgactcgcc ggagggcgat
     3661 atggctacac cctattggca gatgcgtcgg gaagaggttc cttcgactca ggacctggcg
     3721 cgctccgagc tggccactct gcccgtggtg gtggtcgcgg cagtccagac cgcagggcgg
     3781 ggtcgcaccg gtgctgggtg gatcacggcg cctcaggcgc tggcggtgtc ggtggcaatt
     3841 cctatcagcc aaggagagga tcgcccgctg tccctgatgg cgggggtggc agcgaggaga
     3901 gctctcgtcg gggccgtcgg gctgaaatgg cccaacgact tggagatcga tgacctgaaa
     3961 gtgggcggca tcctcgtcga acagagcgac gggattgctg tgattgggtt gggtgtaaat
     4021 ctgtggtggc ctgagccgcc ggaggggatg ggtgcagtgg gcgatgagga ccccggaccg
     4081 caccgtcacc tggaagtggg cgccctgtgg gcggcagagc tccttgccct catcggagcc
     4141 gaagggtggc cgatcgacga gtatcgggaa agttgcggga cgttgggccg agaaatcgcc
     4201 tgggagcctg agggctcggg caaggcaatc gacgtcggcg gatccggtgc cttgctggtg
     4261 caggtcggac acgacatcgt cgaaatccgc tctggcgcgg tccgccacgt ccgcggctag
     4321 cccagcacct cttcttcagc ctccgggaga gtttccttga gaaagccaat gacacgcccc
     4381 atcgcgtcct cggctgccga tgggtcgtat tccccagcgt cgatgtcgag gaatccatgt
     4441 cgcaccccgg gatacagcaa ccactggccg tgctggttcc tgttttgagc ttcgtccacc
     4501 gagctggctg caatcaactc gtcgtcggcg ccgtacaacc caaggacggg aacaggtaga
     4561 tgcccgagga agtcggccac ctggaactcg cggtcctcgt caccggtcag cggggtgtag
     4621 gcaagggcga gggatcggac ccagggcctg gtagctgccg agatcacgcc gaaacggcct
     4681 cccacatcca cgccgaagat cccgatctgg tcgctcaccg cccactcgat gtcatcggag
     4741 acgacgtagc catgcaactc gtccagatcg gcgatggcac gagcgtcgga gaggaggttg
     4801 tagcgatcca aggggtcccc gccggcccgg tagaagtcga cggacaaggc caagagcccg
     4861 ttgcgcgcca gacgacgaca gaggtccttc tcaaagctgg tcaagccatc caggctggga
     4921 agcacgaaca ccacgggaaa tttgccggcg gcgtcaggtc gggcgaggta gccggtccgg
     4981 tgcccggtcc ccatgggaat cgggcaggct ccatacatga tcgggtagat gccttcgtga
     5041 gccaggacgg ccatggtcag acgataaccg gtcaccgggg cccttctcga ccgggcctgg
     5101 gtgttcgggt taggtcggaa ccagagctag cctgcgcgtc gtcctaatca gacgatgacg
     5161 tcctccccat cgactcctgt ttctcgccgc gggctgcccc gttggctcaa agtcaccatt
     5221 ctgactctcc tcgtggtggc caacctcgcc gcgctcggtc tcctttgggc gctgaacacg
     5281 ggccagaacc tgttgagcgg ggcggacacc gaccacgagg tggttgatgt cctcaccgac
     5341 cccaccggag aagcactcac gttcctggtt gtcggcagtg actcgagaga gggactcgac
     5401 gatctggagc acttcggcaa ctttgccggc gcacgcggcg acgtggtgat gctggtcagg
     5461 gtcgacccgt caaccggaga ggcgcgcatg ttgagtatcc cgagggacct atgggtcgac
     5521 gtccccggcc agggcatggg caagatcaac tccgcctatt cctatggcgg gccgagcctc
     5581 atggtcagga ccatccagga gaacttgggc atcgaagtca accactacgt ggaggtcggc
     5641 tttgtgggct ttcaggagtt ggtggacgat cttggaggaa tccacatctc ctttccctac
     5701 gccgccaggg atcgctcgtc cggcctggac gtggaggctg ggaccgacgt cttggacgga
     5761 gagatggctc ttgcctatgc ccggtctcgg aaataccagg agtatcagaa cggctcatgg
     5821 gtgagcgttg acgccaacga catcgggcgg acgcagcggc aacaggaggt agttcgggcg
     5881 attctctccg agctcaagag ccctgcctcg gtcgccgacg ccggcaccat gagcagctcc
     5941 ctggccagcc acatgacgat cgatgccacc ctggcagagg cgtcgatcgc gggcctcgcc
     6001 tgggactttc ggagcctgct gactgggggg atagagggga ccacgctccc ggttgacggt
     6061 gccaccaaag gcggggcatc ggtggtcatc gccagagagc ccgaggcgtc agagatgatc
     6121 gctgacttcc ttggcgggac cacccaggtt tcccaggagc cgcttcgggt tcaggtgctc
     6181 aacggaaacg gtgtcagtgg ctccgccggc aagatgtcgg atgcacttca ggagagcggg
     6241 ttcgaggtgg agtcgatagg caatgccgac agccgcgact acgagcagac cacgatcgtt
     6301 gtcccctcgg gcagccccaa tggaggcgtc atcgcctccg aactgggttt tggtgtagtc
     6361 gagttcggcg atgtcgataa cgcatatgac gccatcgtca ttgtcggcgc cgacgctccg
     6421 tgaccatcgg cgtgggcgtg cgcacttcgg ggctggcgcg gttattcgta gactgagggt
     6481 tcaatggata ttgcgataat tggcgctggc tatgtcggtc tcgtgaccgg tacagggctg
     6541 gcccatctcg gccatcgggt tcgaatcggg gaggccgatg gacaacgcgt ggagcgtttg
     6601 cgccgtggaa tcgtccccat tttcgaagac ggtctgcccg cccttctcgc cgaagcggtg
     6661 gatcgagagc tcatcagctt ccatacgtcg aacgtggagg cggccgccga cagccgtctg
     6721 gtgtttctct gccttcccac ccctgaagcc gaggacgggc gtgccgatct ccgctacatc
     6781 cactcggtga tcgacgagtt agccaacgaa gtcgatccgg atgcgatctt cgtggtgaag
     6841 tccaccgtcc cgcctggaac tgtcaccagc ttgcgcaaga ggctggctga tgtcggcagt
     6901 caggctcgaa tcgtctcgca tcccgagttc ttgcgggagg ggcgcgcggt ggaggacttc
     6961 ctggccccag accgcatcgt tatcggtgcc tgggaggagg aagacgcgga tgcggtggcc
     7021 gagatctact cgggtgtcga taccgaagtt gtgaggaccg accccacctc ggccgagatg
     7081 atcaagtacg cgaacaacgc ctacctggcc acccgtctca cctttgtcaa cacgttggcg
     7141 aatgtgtgtg aggcagttgg tgccgacatc atcgacgtgg ttcgcggcat tggcctcgat
     7201 cacaggatcg gcccacact
//
LOCUS       DBNHXU010000219         2134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3839498, whole genome shotgun sequence.
ACCESSION   DBNHXU010000219 DBNHXU010000000
VERSION     DBNHXU010000219.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2134
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3839498"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..788)
                     /gene="truA"
                     /locus_tag="AC161X_05465"
     CDS             complement(57..788)
                     /gene="truA"
                     /locus_tag="AC161X_05465"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR4989033.1"
                     /translation="MPVYRLDLAYDGTGFRGYARQDGQRTIQGELEDALRTLLGVSPS
                     TAVAGRTDAGVHARGQVVSFDTDHELDGPQLARSLNGILGPEIAVSKVTRVDDGFSAR
                     FSATFRRYRYQLSQAETGDPLARSQVWNVGRRLDLAGMRALAEAIAGEHDFSAFCRSV
                     EGKSNIRLVDDARWDEKDDLLTFWIKANAFCHQMVRSLVGLAYDVGRGFIPADSISEI
                     IESGDRSRVVTVAPPHGLTLWQVGY"
     gene            complement(788..1309)
                     /locus_tag="AC161X_05470"
     CDS             complement(788..1309)
                     /locus_tag="AC161X_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989034.1"
                     /translation="MIKMFSRAGRASLLDRKAFTEAFFDGDAPADAAILVSIVAAVGY
                     VGTLLWLGVLGAFSIANLFQIVLGGVVSWLVLGFATWFVANRLFGASSRPQTLMGIQG
                     LAVLPLILEVFGSIAGGIGLVWYLVILVVGTREATDLSMRNAAVSVLIGFAVAVLIRA
                     LLGVPFAVLSALF"
     gene            complement(1394..>2134)
                     /gene="coaA"
                     /locus_tag="AC161X_05475"
     CDS             complement(1394..>2134)
                     /gene="coaA"
                     /locus_tag="AC161X_05475"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020016149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HIR4989035.1"
                     /translation="RVQGSHQLASVTDTFLGAPPTPVPFVIGVAGSVAVGKSTTSRVL
                     QELLARWPQHPRVELVTTDGFLYPNRVLEERGLMDRKGFPESFDRRALVDFVARAKAG
                     DDHLETPVYSHIAYDIVPDEMRVVMRPDILIVEGLNVLQAGDGGAFVSDYFDFSIYVD
                     ADTAHIRKWYIERFLTLRDTAFTQSDAYFRRYAGLSDEEAAGVALDIWERINEVNLIE
                     NILPTRERASLILEKAEDHSVQLVRLRR"
BASE COUNT          459 a          684 c          649 g          342 t
ORIGIN      
        1 tcatgaccct tggccagaga gtctcgtctc gcgggttcga cccaggccga atctgatcaa
       61 tagccgacct gccacagggt cagcccgtgt ggtggggcga cggtgaccac ccggctccga
      121 tccccggact cgatgatctc ggagatcgaa tccgctggga tgaatccacg gccaacgtcg
      181 taggccaggc caaccaggga tcgaaccatc tgatggcaga aggcgttggc cttgatccaa
      241 aaggtcagca gatcgtcttt ctcatcccat cgcgcatcgt caacgagacg gatgttgctc
      301 ttgccctcaa cggaccggca gaaggcgctg aagtcgtgct cacccgcgat cgcctcggcc
      361 aaggcccgca ttccggccaa atccagccgg cgtcccacat tccagacctg gcttcgggcg
      421 agaggatcgc cggtctccgc ctgcgacaac tgatatcgat aacggcggaa cgtcgctgag
      481 aaccgggcgg agaaaccgtc gtcaacccgg gtcaccttcg aaaccgctat ctccggacca
      541 aggatcccat tcaaggaccg ggcgagttga gggccgtcga gctcgtggtc ggtgtcgaag
      601 gagaccacct ggcctcgggc atggacaccg gcgtccgttc ggcccgccac cgccgtggaa
      661 ggcgataccc cgaggagtgt gcgtagagca tcctcgagct cgccctggat ggtgcgctgg
      721 ccgtcctgtc gggcatatcc gcggaacccg gtgccgtcat aggccaggtc gagccgatag
      781 acaggcatca gaacagagcg ctgagcacgg cgaacggcac gcccaacaag gccctgatca
      841 aaaccgccac tgcgaacccg atcagaaccg acactgcggc attgcgcatg ctgaggtccg
      901 tcgcctcgcg ggtgcccacc accaggatca ccagatacca gaccaggccg atgccaccag
      961 cgattgaccc aaacacctcc aggatgagcg gaagaaccgc caacccctgg atgcccatca
     1021 aggtctgagg gcgcgacgag gctccgaaca ggcgattggc gacgaaccag gtggcgaatc
     1081 cgagcaccag ccaggagacc accccgccaa ggacgatctg gaagaggttg gcgattgaga
     1141 acgccccaag caccccaagc cacagcagag tgcctacgta ccccaccgca gcaacgatcg
     1201 aaacgaggat tgcggcatcc gcaggagcat ccccgtcgaa aaaggcctca gtaaacgctt
     1261 tgcggtcgag aaggctggct cggccggcgc gggagaacat cttgatcaaa gcgaggcgag
     1321 ggtaccgctt ccaaattgcg aaccagaggt ccgaatatga ccgctgcggg acacaattac
     1381 gtggggatcc cgctcaacgc cggagtcgca ccaactggac cgagtggtct tccgccttct
     1441 ccaggatcag cgatgcccgt tcccgggtgg gaaggatgtt ctcgatgagg ttgacctcgt
     1501 tgattcgctc ccaaatgtcg agagcaaccc ctgccgcctc ctcgtctgac aatccggcgt
     1561 accgccggaa ataggcgtcg ctttgtgtga atgcggtgtc gcgaagagtc aggaagcgct
     1621 cgatgtacca cttgcggata tgggcggtgt cggcatcgac gtagatcgag aagtcgaagt
     1681 agtcggagac gaaggctccc ccgtcaccag cctgcagaac gttgagcccc tccacaatca
     1741 ggatgtccgg cctcatcacc acccgcatct cgtcgggcac gatgtcgtac gcgatatgcg
     1801 aatacaccgg cgtctccaga tggtcgtctc cggccttggc tcgagcaacg aagtcgacga
     1861 gtgcccttcg atcgaagctc tcggggaagc cctttcggtc catcaggcca cgctcctcga
     1921 gtacccggtt gggatagagg aagccgtcag tcgtaaccag ttcgaccctc gggtgttggg
     1981 gccagcgggc gagaagctcc tgcaacacac gtgaggtggt cgacttgccc accgccaccg
     2041 agccggcgac tccgatgacg aacggcactg gtgtgggcgg agcccccaag aaggtatcgg
     2101 tcaccgacgc gagctggtga ctgccctgca cgcg
//
LOCUS       DBNHXU010000220         3075 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4036958, whole genome shotgun sequence.
ACCESSION   DBNHXU010000220 DBNHXU010000000
VERSION     DBNHXU010000220.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3075)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3075
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4036958"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            278..853
                     /locus_tag="AC161X_05480"
     CDS             278..853
                     /locus_tag="AC161X_05480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4989036.1"
                     /translation="MEVGIRNSRVLSNEEARFADLYRRHGKQLHAYCLRRTSGSNAAD
                     AVAETFLVAWKKIEMVPEGEAALAWLYAVAYRVLSHQWRHKARSRRLIERLSGLTDSE
                     PPPPDVLLVRSEEYRMVVKASSRLKPIDQEILRLTLWEELSHADVGVVLGIEASAVKQ
                     RAYRARRNLAIEYQKLTKDRQPPTALEGGES"
     gene            850..1623
                     /locus_tag="AC161X_05485"
     CDS             850..1623
                     /locus_tag="AC161X_05485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989037.1"
                     /translation="MTNEDHIVALFAKANPVPSLDLLDPVEALDIERLTHPSERSSAM
                     TDVEAIQPKKGERQSGSRLLLGLAVAVIAFIALGIFVTKENAAATPESVANAYMEARE
                     DLDSETAQSLFAEGATMSEEGFTLPQLPGLFSWYQASNWNWTPGECTEASTGPKGTLV
                     RCSYEFENDWTRALGHAAVTGNLQLQVSEGEITKLTSALDTGQFGDVWEGFLTWMIEN
                     HPDDFDQMYVSGGTAPLIDPTSIALWEQHTEEFVASLEN"
     gene            complement(1708..2487)
                     /locus_tag="AC161X_05490"
     CDS             complement(1708..2487)
                     /locus_tag="AC161X_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007538285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR4989038.1"
                     /translation="MTWLSSEDRGPVRWTTIDRPDRKNAIPFDGWGELRSSFEDFEAS
                     EQRILVLTGAGGEFCAGADLDPSRLEVLQSITDRHQRMKEIGSAALTLHRLTKPTIAA
                     VDGVAVGAGMNLALGCDLVVATERARFSEIFVKRGLTVDFGGSWLLPRIVGLQRAKEL
                     ALSGRIIGAEEALSIGLVLELVPVEGLETRVTELAESLLAGAPMGQTFVKQTLNASFE
                     SSMADALGWEGQAQAVALGTEDMAEGVAAFLEKRPPVWKGR"
     gene            2622..2840
                     /locus_tag="AC161X_05495"
     CDS             2622..2840
                     /locus_tag="AC161X_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989039.1"
                     /translation="MGDLLGVTVSPVQPYAARKEYLCPGCQNTIPPGVFHLVVVPDDE
                     PDLRRHWHHGCWHKEMRRRFGVTQASRR"
     gene            complement(2822..>3075)
                     /locus_tag="AC161X_05500"
     CDS             complement(2822..>3075)
                     /locus_tag="AC161X_05500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989040.1"
                     /translation="VGPAIGAVIAFSFAESILAIWAPYQNVSLSAATNGLFDVEMGGS
                     FGAFVPGGDLSQAHALAIIAGWTILGLALTWWGLQRRDA"
BASE COUNT          618 a          974 c          917 g          566 t
ORIGIN      
        1 tgaccgactg gattgcagcc aaccatccgg acacggtcga tcaggtcctc acccccgacc
       61 tgcggaggcc cgtcctcgac gccaactcgg tcgcactttg ggagcagtac accgaggagt
      121 atgtcgcgtc atccacgggt tgagcccacg tccgcggtcc gtgggatcaa ccaggcaacc
      181 ccccggttgc tctcatatcg ttctatgccc ggatgagggg tgatggtcac ccaaaatatc
      241 tctgttacat gtcgagccgg ccgggcatag acatctcatg gaggtcggca taaggaactc
      301 gcgagtcctc tcgaacgagg aagccagatt tgctgacctc taccggcgtc acggcaagca
      361 gctccatgct tattgcctcc gacggacgtc aggatcaaac gcagccgatg ccgtcgccga
      421 aaccttcttg gtggcatgga agaagatcga aatggttccg gagggagagg cagcacttgc
      481 gtggctctac gcggtggcct atcgggtcct ctcccaccaa tggcgacaca aggcacgcag
      541 ccggaggctg atcgagcgac ttagtggtct gaccgattcc gaacccccac cccccgatgt
      601 tctcctggtc agaagcgagg agtatcggat ggtcgtcaag gcgtcgtctc ggctcaagcc
      661 aatcgatcaa gagatcttgc gtctgacgct ctgggaggag ttgtcacacg ccgacgtcgg
      721 ggtggtgctc gggatcgagg ccagcgctgt caaacaacgc gcctatcgcg ctcgtcgcaa
      781 cctcgcaatc gaataccaga aactaaccaa ggaccgtcaa ccccccactg ccctcgaagg
      841 aggtgaatcg tgaccaacga agatcacatc gtggccttgt tcgccaaagc gaacccggtt
      901 ccgtcgctgg atctactcga cccggtcgag gctttggaca tcgaacgcct cacccaccca
      961 tccgagagga gcagcgccat gaccgacgtc gaggctattc agcccaagaa aggtgagcgg
     1021 caatcgggat cacgacttct tctcggtctg gccgtggccg tgatcgcatt catagccctg
     1081 gggatctttg tcaccaagga gaatgcagct gccacacccg aatcggtcgc caacgcctat
     1141 atggaggctc gggaggatct cgattctgaa acggcgcagt ccctgttcgc tgagggtgcg
     1201 accatgagcg aagaggggtt cacccttccc caattgcctg ggttgttcag ctggtatcag
     1261 gcgtccaact ggaattggac ccctggcgag tgcacggaag cctcaacagg gccaaaagga
     1321 actcttgtca gatgcagtta cgagttcgag aacgactgga ctcgcgcgct ggggcacgcg
     1381 gcggtgaccg gcaaccttca gctccaggtg tcagagggtg agatcaccaa gctgacctcc
     1441 gctctcgata ccgggcaatt tggcgatgtt tgggagggct ttctgacttg gatgattgag
     1501 aaccatcccg acgacttcga ccagatgtat gtctcgggcg gcacggcccc gctgatcgat
     1561 cccacctcga tcgctctctg ggagcaacac accgaggagt tcgtggcctc acttgagaac
     1621 tgacccctag gagcccacag gtcacggaga cgtaccatcg tctccgtgac cttggaagtc
     1681 gccattgtcg gaaccggaaa ggtcttttca acggcctttc cacaccgggg ggcgcttctc
     1741 gagaaaggcc gccaccccct ccgccatgtc ctcagtgccg agggcgacgg cttgggcctg
     1801 gccctcccat ccgagggcgt cggccatgct cgactcgaac gatgcattga gggtttgctt
     1861 cacgaatgtc tgacccatcg gcgcccctgc cagcaatgac tcggcgagct ctgtcacccg
     1921 cgtctcgagg ccttccactg ggaccagctc gaggaccagc ccgatactga gcgcctcctc
     1981 agctccgatg atgcgcccgg aaagcgccaa ctccttggcc cgttgcagcc cgacgatccg
     2041 gggcaggagc cacgacccgc cgaagtccac ggtgagccct cgcttgacga agatctcaga
     2101 gaaacgggct cgttcggtgg ccacgacgag atcgcacccg agagcgagat tcatcccggc
     2161 gccaactgcg accccatcga cggctgcgat ggtcggcttg gtcagtcggt gcaacgtgag
     2221 agccgccgat ccgatctctt tcatcctttg atggcggtcg gttatggact gcaacacttc
     2281 gagacgactg gggtcgagat cggccccggc acagaattcc ccgcccgctc cggtcaacac
     2341 caggatccgt tgctcggagg cctcgaaatc ctcgaatgag gaacgaagct caccccaacc
     2401 gtcgaacggg atcgcgttct ttcggtcagg acggtcaatt gtcgtccatc gcacagggcc
     2461 gcggtcctcc gatgagagcc aggtcatagg ttgacggtag accgataaca gggccgttac
     2521 cgttcgcctg tgggtttcaa tccttttcgc gagcagaaca agagcgcggc cgatatcgcg
     2581 gccatggtca tcgcgctcct tgcgatcctg gcggcggtgg catgggcgat cttctcgggg
     2641 tgactgtcag cccggtgcag ccctatgcgg cccgcaagga gtacctctgc ccgggttgtc
     2701 agaacacgat cccgccagga gtgttccatc tcgtcgtcgt ccccgatgac gagccggacc
     2761 ttcgccggca ctggcatcat ggctgctggc acaaggaaat gcgacgccgc ttcggcgtca
     2821 ctcaggcgtc gcggcgctga agaccccacc aagtcagggc gagtcccaaa atcgtccatc
     2881 cggcgatgat ggcgagggcg tgggcctggc tgaggtcccc accggggaca aatgccccga
     2941 acgagcctcc catctcgacg tcgaacagcc cgttggtggc cgccgacagt gaaacgttct
     3001 gatagggagc ccatatggcg aggatgctct cggcgaacga aaaggcgata accgcgccga
     3061 tggccgggcc caccg
//
LOCUS       DBNHXU010000221         2970 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_270278, whole genome shotgun sequence.
ACCESSION   DBNHXU010000221 DBNHXU010000000
VERSION     DBNHXU010000221.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_270278"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..576
                     /locus_tag="AC161X_05505"
     CDS             157..576
                     /locus_tag="AC161X_05505"
                     /inference="COORDINATES: protein motif:HMM:NF017935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HIR4989041.1"
                     /translation="MSHDQVVKGRTRIWRGLRRRCPRCGAPAFDTYFGMKEDCSQCGI
                     HFEREEGYWVGALIINTTVTFATFLLIFVGGIVITWPDVPWTGVGVVTIVANGLIPVA
                     FYPVSKTLWLALEMGWHPLEPEEVKAARVRSGLRLET"
     gene            complement(573..1712)
                     /locus_tag="AC161X_05510"
     CDS             complement(573..1712)
                     /locus_tag="AC161X_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013262048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR4989042.1"
                     /translation="MKSVYMTEDLEAVRAQTRTFVEREIVPNAAPWETEGVPREVLGR
                     MGALGFFGLRIPEEYGGVGLGALASVVFAEELGRSTYGGFTITVLVHTDLAAPYVVNF
                     GTPSQKAHWLPKFATGDAIAAIAVTEPDAGSDVAAIRTSARREGDGFVINGTKMFITN
                     GALADVVFVAAKTNPEVKGSRGVSIFAVERGAKGFSTGRALEKMGWRSSDTSELVFDE
                     VWVDEDSLIGEENQGFYYIMENFQNERLAIMGQALGESQRALELTIEHVKGRRAFGSP
                     LWDKQAIRQRIAMRQTEIDAGRELTYRAAWLTDQGEDAVREVSEVKAYVGELANRVLY
                     DCVQFHGGMGFIAETAVERLYRDVRIASIGGGATEIMLDEVAKRL"
     gene            complement(1709..2620)
                     /locus_tag="AC161X_05515"
     CDS             complement(1709..2620)
                     /locus_tag="AC161X_05515"
                     /inference="COORDINATES: protein motif:HMM:NF004283.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA lyase"
                     /protein_id="HIR4989043.1"
                     /translation="MSNLPSRVTVTESSSRDGLQSLGVTIPTRSKVELIEALAGTGLR
                     SFDAVSFVSAKAVPQMADGAEVLAGLSQRDDLELWGLVPNMKGLESALEAGLTSIGLL
                     TAASDTFNQRNINATVDESMHRIRRILTEAPGGLRFRGYVSTATHCPFEGEQEPDWVA
                     HLVETLLEWGVDEVFLGETLGKATPAHVERLLDEVLDLVTVEKVGVHFHDTYGQAIAN
                     TMVALERGIDKMDSSAGGLGGCPYAPGATGNVATEDLLWMLDGLGVSHDIDIVGVARV
                     ARDFCANNELNYASKAGRALLAAEGLS"
     gene            complement(2660..>2970)
                     /locus_tag="AC161X_05520"
     CDS             complement(2660..>2970)
                     /locus_tag="AC161X_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989044.1"
                     /translation="QDLRPIERVIVRLSEAGQEPPEISRRVGKKPGTVTRIMTMIDLR
                     SESPMTHVVDDQALRPVERVVLKLRARGESYGEIGIRLRKSGEQVRRIESYAQLKLEA
                     "
BASE COUNT          548 a          980 c          870 g          572 t
ORIGIN      
        1 gttgatccct gatgcgaccg aggcggtgaa ccaattctcg aactgggaag tcgggtcggc
       61 tgccatctca ccctccagga gacctcgtgc ctcgtactcc cgtctgaggc gggcgatccg
      121 gtcgtcactc accctgccga cgatagcctc gccgatgtga gccacgacca ggtagtaaaa
      181 gggcgcacca ggatttggcg agggctgagg agacgctgtc ctcgttgcgg cgctccggcc
      241 ttcgacacct acttcggtat gaaggaggac tgcagccaat gcggcatcca cttcgaacgc
      301 gaggagggct actgggttgg ggcgctgatc atcaacacga cggtgacctt cgccacgttt
      361 ctcctgatct tcgtcggtgg catcgtgatc acctggcccg acgtcccttg gacgggcgtg
      421 ggagtggtca cgatcgtcgc caacgggctc atccccgtcg ccttctaccc ggtgtccaag
      481 actctgtggc ttgccttgga gatgggatgg caccccttgg agccagagga agtaaaagca
      541 gcgagagtgc gctcgggctt gagactcgag acctaaagcc tctttgccac ctcatccagc
      601 atgatctcgg tggctccccc gccgatgctc gcgattcgga catcgcgata gaggcgctcg
      661 actgcggtct cggcaatgaa gcccattccc ccatggaact ggacgcagtc gtagaggacc
      721 cgattggcaa gctcgcctac atacgccttc acctccgaca cttcgcgcac ggcgtcctcg
      781 ccctggtcgg tcaaccatgc cgcccgataa gtcagctcgc gaccggcgtc gatttcggtc
      841 tgtctcatcg cgatacgttg tcggatcgcc tgtttatccc acagcgggga accgaaggcc
      901 cgtctccctt tgacatgctc aatggtcagc tcgagagccc gttgcgattc ccccagcgcc
      961 tgacccatga tcgccagtcg ctcgttctgg aagttctcca tgatgtagta gaaaccctgg
     1021 ttctcctcac cgatcaggct gtcctcatcg acccacactt cgtcgaagac cagttcgctc
     1081 gtgtccgacg accgccaacc catcttctcg agggcccgcc ccgtgctgaa gcccttggct
     1141 cccctctcga cggcgaagat cgaaacccca cgagatccct ttacttctgg gttggtcttg
     1201 gccgcgacaa acaccacatc ggccagggcc ccgttggtga tgaacatctt ggttccgttg
     1261 atgacgaaac cgtcgccctc gcgacgcgca ctagttcgga tcgctgcgac atccgacccg
     1321 gcgtcaggct cggtgacggc gatggcggcg atcgcatccc cggtagcgaa cttgggcagc
     1381 caatgcgctt tctgtgaagg cgtgccgaaa ttgacgacat agggagcagc aaggtccgtg
     1441 tggacgagca cggtgatcgt gaacccccca taggtggacc tgcccaactc ctcagcgaac
     1501 accaccgatg ccaatgcgcc gaggcccacc cctccgtatt cctcggggat gcgcaacccg
     1561 aagaacccga gtgctcccat cctgccaaga acctctctcg ggacgccctc ggtctcccag
     1621 ggagcggcgt tggggacgat ctcgcgctcg acaaacgtgc gggtctgcgc cctcaccgct
     1681 tcgaggtcct cggtcatata gaccgacttc aagagaggcc ctcggcggcg agcagggcgc
     1741 gtcctgcctt gctggcgtag ttgagctcgt tattcgcaca gaaatcccgg gcgacacgag
     1801 ccacgccgac aatgtcgatg tcatggctca cccctagccc gtccaacatc caaaggaggt
     1861 cctcggtggc cacgttgccc gtcgcgcccg gggcataggg acaaccaccc aaaccgcctg
     1921 ctgatgaatc catcttgtcg atgcctcgct ccagggccac catcgtgttg gcgatcgcct
     1981 gtccataggt gtcgtgaaaa tgcacgccga ccttctccac ggtgactagg tcgagcacct
     2041 catcgaggag cctctcgacg tgcgcggggg ttgccttgcc gagagtctca ccgaggaaga
     2101 cctcatcgac tccccactcg agcagggtct caacaaggtg agccacccaa tcgggctctt
     2161 gctcaccctc gaaaggacaa tgggtggcag ttgacacata gccccggaat cggagtcccc
     2221 ccggcgcctc cgtgagaatg cgacggatcc ggtgcatcga ctcatcaact gtggcgttga
     2281 tattgcgctg gttgaaggtg tcagaggccg cggtcaggag cccgatgctg gtcagaccgg
     2341 cttcgagggc agactccagg cccttcatgt tgggcaccag cccccaaagc tcgagatcgt
     2401 cccgctggct aagtcccgcg aggacttcgg caccgtccgc catctgggga acggccttcg
     2461 cactgacaaa tgagacggca tcgaatgacc gcagacctgt ccccgccagg gcctcgatca
     2521 gctcgacctt ggaccgggtg ggaatggtga cgcccaggga ttggagccca tcacgagaag
     2581 aggactccgt gacagtgacc cttgagggga ggttgctcac gtccgggacg gtacccggcc
     2641 cgcgtgggca atctcgggct caggcctcca gcttgagttg ggcgtaactc tcgatccgcc
     2701 gaacttgttc gccgctcttg cgcaggcgga tcccgatctc tccgtagctc tcgccacggg
     2761 cccgcaactt cagaacgacc ctctcgactg gcctgagagc ctgatcgtca acgacgtgag
     2821 tcatcggtga ctcggacctc agatcgatca tcgtcatgat ccgggtgacg gtcccgggct
     2881 tcttaccgac gcggcgcgaa atctcaggcg gctcctggcc ggcttcgctg aggcggacga
     2941 tcactcgctc gattggtcgt aggtcctggt
//
LOCUS       DBNHXU010000222         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4633715, whole genome shotgun sequence.
ACCESSION   DBNHXU010000222 DBNHXU010000000
VERSION     DBNHXU010000222.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4633715"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            169..1452
                     /locus_tag="AC161X_05525"
     CDS             169..1452
                     /locus_tag="AC161X_05525"
                     /inference="COORDINATES: protein motif:HMM:NF013584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HIR4989045.1"
                     /translation="MAGDTASRRVEQSLQLAHAAQPTLNAFTLIDDERALSRAGEIDE
                     AGTGGLLAGVPIALKDLIDHQGRVTTCGSAFYRHLATTTAPAVTRLEDAGGVVISRAG
                     LHEFAFGFSSENQHFGPVRNPWDPTTSPGGSSGGSGAAVAAGITPIAIGTDTGGSVRV
                     PAALCGCFGLKVTPGRIPLDGVFPLVPSIDTVGPLADSMAGLETAYRVLSGARQGGSG
                     WTRLRIGIPQPWYDEAPADDEVDAAFARIVGLLRELGHEVRAVSLPSVLPGREVIHAI
                     ADEVSQVHHDFREKSLPYGDEVAARLDDCATVTPDQSAQGRNWQQMIRTRFSDAFEQV
                     DLIITPTVPVMRKVIGVDNIGDLHYRAVLSWFTSIVNHALLPAIAMPLIGAGSPPVSL
                     QAIGPMGSEEMLLGFGGDLESQGVVGFVPPTVQLG"
BASE COUNT          316 a          563 c          527 g          301 t
ORIGIN      
        1 gaccgagcag gattggtaca cgttgaccga cgccgatggc aacaacatcg ccgtcccacc
       61 cggccaggtc tggatctcac tcgttcccga catcggcgga ctgacctacg agtaggcgcc
      121 aaaggccctt actcagtctc aagtctcaag gcagctagcg tcgacggctt ggccggagac
      181 acagcttcca ggcgggtcga acagtcgttg caactagcgc atgcggctca gccgacgctc
      241 aatgcattca ccctgatcga cgatgagcgc gccctctccc gcgccgggga gatcgacgag
      301 gcgggcactg gcgggctgtt ggccggagtc cccatcgcct tgaaggacct catcgaccac
      361 cagggccggg tgaccacctg cggttcggcc ttctaccgcc accttgccac gacgacggca
      421 ccggcggtga cgcgtctgga ggacgccggg ggcgtcgtca tcagccgcgc tgggctgcat
      481 gagttcgcat ttgggttctc gagcgagaac cagcacttcg ggccggtccg caatccatgg
      541 gatcccacca cctcgcccgg gggctcttca ggaggaagcg gcgccgctgt cgccgcgggc
      601 atcaccccca tcgctatcgg caccgacacc gggggctcag tccgggtccc agccgcgctg
      661 tgtggttgct tcggcctgaa ggtcactccg ggccgcatcc ccctggatgg ggtgttccct
      721 ctggtcccgt cgatcgacac ggtcggaccg ctcgcagatt cgatggctgg tctcgaaacc
      781 gcctatcggg tgctgtcggg tgctcgacaa ggagggtcgg gctggacccg gcttcggatt
      841 ggaattcccc aaccctggta cgacgaagca ccggcagatg atgaggttga tgctgcgttc
      901 gcccggattg tcgggctctt gagagaactg ggccacgagg tgagggctgt ttcgcttccc
      961 agcgtgctgc ccggacgcga ggtcatccac gccatcgccg acgaggtcag ccaggtccat
     1021 cacgacttcc gagaaaagag ccttccctac ggggacgagg tggccgcgcg attggacgac
     1081 tgcgcgaccg tcaccccgga ccagtcggcg cagggccgga attggcaaca gatgatccgc
     1141 acccggttct ccgacgcctt cgagcaggtg gacttgatca tcactccgac tgtccccgtc
     1201 atgcgcaagg tgatcggcgt cgacaacatc ggggacctcc actatcgagc agttctctcc
     1261 tggttcacct cgatcgtcaa ccacgcgctt cttccggcga tcgcaatgcc cctcatcgga
     1321 gcgggaagcc cgccagtgtc cctgcaagcg atcggcccga tgggctccga ggagatgctc
     1381 ctcggattcg ggggcgacct ggagtcccag ggggtggtcg ggttcgttcc tccaaccgtt
     1441 caacttgggt agccctccgg gccgataaaa taggtccagc gagccaccga gattccgacc
     1501 ttttaccaga accaggtgtt caatagctct ggtgtgcgcg ccaacgaata gcaataagga
     1561 agtgaaccca aagtggctga gcaaatcgcc acagcaaaac ccccgacccc cgataccgag
     1621 acctttgaca agatagttat ccgattcgcc ggcgactccg gcgacgggat gcaagtagcc
     1681 ggatccagat tcaccgacgc cagtgcc
//
LOCUS       DBNHXU010000223         5316 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_467701, whole genome shotgun sequence.
ACCESSION   DBNHXU010000223 DBNHXU010000000
VERSION     DBNHXU010000223.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5316)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5316)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5316
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_467701"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..748
                     /locus_tag="AC161X_05530"
     CDS             <1..748
                     /locus_tag="AC161X_05530"
                     /inference="COORDINATES: protein motif:HMM:NF014075.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HIR4989046.1"
                     /translation="EYAETIFDSRALAQFKDRHRGERVVIIGNGPSLNDLDLTRLKSE
                     YTVAVNGIFYAADKMGFDPSFYVVEDTAVMRDNLSEIVAYSAGHKFFPSIYRAMVGDL
                     PDVTYFMMNRGFYEPRSPNFCVPRFSMDPGQRLYSGQSVTLINLQLAYHLGFAEVVLI
                     GMDFSYTVPPEAIVEGDVITSTGDDPNHFHPDYFGAGKVWKDPKLDRVLANYALAKLI
                     FEADGRRILNATAGGRLELFERVDYARHFS"
     gene            793..1518
                     /locus_tag="AC161X_05535"
     CDS             793..1518
                     /locus_tag="AC161X_05535"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR4989047.1"
                     /translation="MKEPVYRYRITDYDRSIVPSRFVAFLDDGVIDNLDWVSRSGRSL
                     GHPGWGWVYHTILMLLDADRPNVMVETGTNVGSTAILIGQAILDSGREGVLHTIELDP
                     QIHEEAKRRFDLAGVSSVIKAYCGDALQVLPDVVAGIDEIAVAFLDGNHFHDHVVEEF
                     SVVVDRMRPDGAVIFDNTGLIGEGKEDPRVNGALRTIVSKHGGSLVNLPFCSWYTPGI
                     AIWQRQPFEDMEPPLPGSFTPNS"
     gene            complement(1523..2068)
                     /locus_tag="AC161X_05540"
     CDS             complement(1523..2068)
                     /locus_tag="AC161X_05540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989048.1"
                     /translation="MTISNLVFSNRSSRIALVGITALVSIGWLFVDSSTALRILGLLT
                     ALLLVVAIKTCAELFVQLRTAKKKALHAMESAAQSKRSVDESRAELDMARQRLTRLEA
                     AMFEERGAFSQERQRTEARIRELRSEMSKTVKELSTRVDQLANRSGTRRHRETTESFR
                     ELGLRDSLAGLSRPEASESTE"
     gene            complement(2089..2784)
                     /locus_tag="AC161X_05545"
     CDS             complement(2089..2784)
                     /locus_tag="AC161X_05545"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR4989049.1"
                     /translation="MIERVVVTGAQGGIGSACVDGFRDLGAEVIGVDLVEGSPEDLLL
                     DLSDPDCGSQLLAHLAGRSVDVLVNNAAMGFSGEAVDTDAAEFDRLIAVNLRAPFLLS
                     AALYPSLRQRSGAVVNVSSVHAVATSSRVSVYAASKGGLVSMTRALALEWAPEVTVNC
                     VLPGAVDTEMLTDGLFRAGATVESFGERTPARRVAQPSEIAQAVVFLATNRFTTGAAL
                     VVDGGASARLSTE"
     gene            complement(2781..3722)
                     /locus_tag="AC161X_05550"
     CDS             complement(2781..3722)
                     /locus_tag="AC161X_05550"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HIR4989050.1"
                     /translation="MADPDRVLITCGLVWDAGDEVIRRLEAVGLEADMPRFEGQQLGE
                     EDLLPIIGRYAGILAGDDQLTRRVMEAGPGLRVISKWGIGVDAIDLEAAADLGIRVFN
                     TPGVFGDELADYAMGYLHLLARRQHEVNQQVKDGTWHKVRGRSLAGMTIGIVGMGSSG
                     RSLAQRASAASMRVLGYDTVVPEPGTSFELVSLERLLAEADVVSLHVPASKETHHLIN
                     ANTLSQMKQGAWLINTSRGALVDEAALVDALREGQIAAAALDVFEDEPVSPANPLLSF
                     ESVVVGAHNGSNTVEAVARTTDAAVANLIKGLGLDEG"
     gene            complement(3715..4377)
                     /locus_tag="AC161X_05555"
     CDS             complement(3715..4377)
                     /locus_tag="AC161X_05555"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: protein motif:HMM:NF014409.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HIR4989051.1"
                     /translation="MSLAGLIPMKGHSERVPRKNLRMIAGRPLFHWITATLLNASLVD
                     EVIVDTDSDEIEESVRESFPNVRIHRRPLHLHGDLVAMHDVVTEVARTLDHDNLLQSH
                     TTNPLLRAATVDGAIDAYLGRGGHDSLMSVTPLQTRLYFESGEPVNHDPAVLLRTQDL
                     PPILEENSCIYIAPTALVVATGRRTGPNPLLFSIPKVEAVDIDDEVDFAVAEALLERS
                     SG"
     gene            complement(4374..>5316)
                     /locus_tag="AC161X_05560"
     CDS             complement(4374..>5316)
                     /locus_tag="AC161X_05560"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR4989052.1"
                     /translation="VAIRRSDHGCDPGRQRLMSPRVSIVIRCHNEAEHIGKLLAELKR
                     QTFTDYEVVVVDSGSDDGTLDIVSNEDVILEHIRKEDFSFGRSLNIGCSAATGEILVF
                     ISAHCYPEHEDWLANLINGFDDDKVAVVYGKQRGIPESHFSERQIFRSWYPDVSVPRQ
                     EGPFSNNANAAVRRALWEMHPYDEPLTGLEDVAWAQEVMRDGWWVSYRADAGVVHVHN
                     ETSGQIRNRYRREAITFQKVFPGEHFNTWDFLRLSSRNVLADSRAAFDEGVGLSSLWP
                     IVRFRVSQFTGTYQGFHMRRPPSSDLKKRFYYPEEAE"
BASE COUNT         1051 a         1697 c         1568 g         1000 t
ORIGIN      
        1 ggagtacgcg gagaccatat tcgactcccg cgccctcgct cagttcaagg accggcatcg
       61 gggggaacgg gtcgtcatca tcggcaatgg gccctcgttg aacgatctgg atctgaccag
      121 gctgaaaagc gaatacaccg tcgctgtgaa tgggatcttc tatgcggcgg acaagatggg
      181 cttcgatccc agcttctacg tcgtcgagga caccgccgtc atgcgcgaca acctgagcga
      241 gatcgtcgcc tactccgccg ggcacaagtt ctttccatcg atctaccggg ccatggtggg
      301 tgacctaccg gacgtcacct atttcatgat gaaccgcggt ttctatgagc cccgctcccc
      361 aaacttctgc gtcccccggt tctctatgga tcctggtcag cggctgtaca gcgggcagtc
      421 ggtgaccttg atcaacctcc agctggccta tcaccttggc tttgcggagg tggtgctcat
      481 cggcatggac ttctcctaca cggtgccccc cgaggcaatt gtcgagggtg acgtcatcac
      541 ttcgaccggc gatgatccca accacttcca ccccgactat ttcggagccg ggaaggtgtg
      601 gaaggatccg aagctggacc gggtgctcgc caactacgcc ctggccaaac tcatcttcga
      661 agccgacggc cgccggatcc taaatgcaac cgccggaggc cgcctcgagc tctttgaacg
      721 tgtcgactac gcccgacatt tctcctagac ccgattcggc gagagtctga tcgagcttct
      781 tagagtgcgc tggtgaaaga gcccgtatac cgctaccgaa tcacggacta cgaccggtcc
      841 atcgttccga gccggttcgt tgccttcctc gacgacggcg tcatcgacaa tctcgactgg
      901 gtcagccgct cgggacgcag cctcggccac ccaggatggg gctgggtcta ccacaccatc
      961 ctgatgctgc tcgacgcgga ccggccgaac gtcatggttg agaccgggac caatgttggc
     1021 tccacggcaa tcctcatcgg acaggccatc ctggactcag gtcgcgaagg cgtgttgcac
     1081 accatcgagc tagatccaca gattcacgaa gaggcgaaac gccggttcga cctggcaggt
     1141 gtgtcgagtg tgatcaaggc gtattgcggt gacgcattgc aggttctccc cgacgtcgtc
     1201 gccgggatcg acgagatcgc cgttgcgttc ttagacggca atcattttca cgaccacgtg
     1261 gtcgaagaat tcagcgtcgt cgtggatcgg atgcgccccg acggcgcagt gatcttcgac
     1321 aacaccggtt tgatcggtga aggcaaggaa gaccctcgag tcaatggcgc cctccgcacc
     1381 atcgtcagca agcacggagg cagtctggtg aacctcccgt tctgcagttg gtacacacca
     1441 ggcatcgcca tctggcagcg ccagccgttc gaagacatgg agccgccgct acccggatcg
     1501 ttcaccccga atagctgacc agctattccg tcgactctga tgcctccggc cgggacaggc
     1561 cggcaagcga gtcacggaga ccgagctcgc ggaacgattc ggtggtctca cggtggcgtc
     1621 gagtgcccga gcgattcgcc agctggtcca cccgggtcga tagctccttg accgtcttgg
     1681 acatttcgct gcgaagctca cgaattcgag cctcggtccg ttggcgctcc tgggaaaagg
     1741 cacctctctc ctcaaacatc gcagcctcga gcctcgtcaa acgctgacgg gccatgtcca
     1801 actcagccct gctctcgtcg acggagcgct tcgactgagc cgcactctcc atcgcatgga
     1861 gcgccttctt tttcgccgtc cgcagctgga caaacaactc ggcgcacgtc ttgatggcta
     1921 caacgagaag caacgcggtc agcagtccga ggatcctcag ggcggttgag ctgtcaacaa
     1981 aaagccatcc gatcgacacg agcgcggtga tcccgaccag cgcgatccgg gagctccggt
     2041 tcgagaagac gaggttgctg atggtcattt gagctctctt tccactcttc actctgtgct
     2101 cagtcgagcc gacgctccac cgtccacaac gagggctgcc ccggtcgtga accgattggt
     2161 cgccagaaac acaacggctt gcgcgatctc cgaaggctgg gcaaccctcc tcgccggcgt
     2221 cctctcgcca aatgactcca cggtggctcc tgcccggaaa agaccgtcag tgagcatctc
     2281 agtgtcgact gcaccgggca gcacacaatt gacggtgact tccggtgccc actccagggc
     2341 cagggcgcgg gtcatggaaa caaggccacc cttggatgcg gcgtacacac tcacccgaga
     2401 agaagtagcc accgcatgaa cagacgacac gttgaccacc gcaccggagc gctggcgcag
     2461 gctcggatac agcgccgctg acaggaggaa tggggcccgt agattgaccg cgatcaggcg
     2521 atcgaactcg gccgcgtcgg tgtccacggc ctcgcccgag aagcccatgg ccgcgttgtt
     2581 aaccagaaca tcgacagacc ggccagcgag atgggccaga agctgagagc cgcaatccgg
     2641 gtccgagagg tcgagtagca agtcctccgg tgatccctcg acaaggtcga cgccgatcac
     2701 ctcggcgcct aggtcgcgaa aaccatcgac acaagcactg cctatgccgc cctgtgcacc
     2761 ggtgaccaca actcgctcaa tcatccttcg tccagcccca aacccttgat gaggttggct
     2821 accgccgcat cggtggtccg ggccaccgct tcgacagtgt tcgacccgtt gtgagcgcca
     2881 acgacaacgc tctcaaatga caacagcgga ttcgcaggac tcaccggctc gtcttcgaag
     2941 acgtccagtg ctgcagccgc gatctgcccc tccctcaggg catcgaccaa cgcggcctcg
     3001 tcgacgaggg cacctcgcga ggtgttgatc agccaggcgc cctgcttcat ctgactcaaa
     3061 gtgttggcgt tgatgaggtg gtgcgtctcc ttgctggccg ggacgtgcaa cgacaccaca
     3121 tcggcctcag ccagcaatcg ttcgaggctc accagctcaa agctggttcc tggttccggg
     3181 accaccgtgt cgtagcccaa cactcgcatc gacgccgccg acgcacgctg ggcgagcgag
     3241 cgcccagagc tgcccatccc gacgatcccg atggtcatcc cggccagcga gcggcctctc
     3301 accttgtgcc acgttccatc cttcacctgc tggttcacct cgtgctggcg cctcgccaag
     3361 agatggagat accccattgc gtagtccgcg agctcgtctc cgaagacacc gggcgtgttg
     3421 aagaccctga ttcccaggtc cgccgctgcc tcgaggtcga tggcgtcgac accaatgccc
     3481 cacttcgaga tgaccctcag acccgggccg gcctccatca cccttcgagt cagttggtcg
     3541 tcgccggcga ggatccccgc atacctgccg atgatgggaa ggaggtcttc ctcgcccagc
     3601 tgttggccct cgaagcgagg catgtcggcc tccaaaccga cggcttcgag acgccggatc
     3661 acctcgtcgc cggcatccca gaccagcccg caggtgatca ggacgcgatc gggatcagcc
     3721 acttgaccgc tcgagaagtg cctcggccac ggcgaagtcg acctcgtcgt cgatgtcaac
     3781 cgcctcgacc ttcggaatcg agaacaacag cggattgggg ccggtccgac gcccggtggc
     3841 cacgacaagt gccgttggtg cgatgtagat acaggagttc tcctcgagaa tcggaggcag
     3901 gtcttgagtg cggaggagga cggccgggtc gtgattcaca ggctcgccac tctcgaagta
     3961 gagacgggtc tggaggggcg taaccgacat cagggaatcg tggccacccc tgccgagata
     4021 ggcatcaatg gcgccgtcaa cagtggccgc gcgaagcagt gggttcgtgg tgtggctctg
     4081 caacagattg tcgtggtcga gagtgcgtgc cacctctgta accacgtcgt gcatggccac
     4141 cagatcgcca tgtaggtgca atggacgacg gtggatcctc acattcggga aggactctcg
     4201 gacagactcc tcgatctcat cgctgtcagt gtcgacgatg acctcgtcga cgagtgaggc
     4261 gttgagcaag gtcgcggtga tccagtgaaa caggggacgc cccgcgatca tccggaggtt
     4321 cttacggggc acccgctcgg agtgcccttt catcgggatg aggcccgcaa ggctcattcg
     4381 gcttcctcgg ggtagtagaa gcgcttcttg agatcgctcg acggcggacg cctcatgtga
     4441 aagccctggt aggtcccggt gaactgtgac acacgaaatc ggacgatcgg ccatagcgac
     4501 gagagcccga cgccttcgtc aaaggcggca cgactgtcgg caagaacatt tctcgacgaa
     4561 agccgaagga aatcccaagt gttgaaatgc tcccccggaa acaccttttg gaaggtgatc
     4621 gcctcccgcc ggtatcggtt gcggatctgt cccgaggtct cgttatggac atggaccact
     4681 ccggcgtccg ctcgatagct cacccaccac ccatctcgca tgacctcctg ggcccaagcg
     4741 acgtcctcga gcccggtgag gggttcgtcg taagggtgca tctcccaaag ggctcgccgg
     4801 accgcggcat tcgcattgtt ggagaaaggg ccctcctggc gaggaacgga gacatcgggg
     4861 taccagcttc tgaatatctg gcgctcagag aagtgtgact cggggatgcc tcgctgcttt
     4921 ccatagacca ctgccacctt gtcgtcgtca aagccattga tgagattggc aagccagtcc
     4981 tcgtgctccg gatagcaatg ggcggaaatg aagacgagga tctcgcctgt cgccgcgctg
     5041 caaccgatgt tgagtgatct gccgaagctg aaatcctcct ttcggatgtg ctccaggatg
     5101 acgtcctcgt tgctgacaat gtcgagagtg ccgtcgtccg agcccgagtc gacgacgacg
     5161 acctcgtaat ccgtgaaggt ctgacgtttc agctcggcga gcagctttcc gatgtgttcc
     5221 gcctcgttgt gacagcggat gacaatggag actctgggac tcatcaggcg ctggcgtccc
     5281 ggatcgcagc cgtgatcgct ccgacgtatg gcgaca
//
LOCUS       DBNHXU010000224         8799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1460673, whole genome shotgun sequence.
ACCESSION   DBNHXU010000224 DBNHXU010000000
VERSION     DBNHXU010000224.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8799
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1460673"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..253)
                     /locus_tag="AC161X_05565"
     CDS             complement(<1..253)
                     /locus_tag="AC161X_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003058941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR4989053.1"
                     /translation="MRFGAFVPQGWRLDLAGIDREQQWATMSGVAMTLETAGFESAWV
                     YDHFHTVPIATQEETYEAWSLMAALAAVTSRIRLGQMCTC"
     gene            complement(340..1212)
                     /locus_tag="AC161X_05570"
     CDS             complement(340..1212)
                     /locus_tag="AC161X_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015942399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR4989054.1"
                     /translation="MTAVASKAPGTTTKSGWAVKLSVFLIVFLWTIPTVGLLVSSFRP
                     ASDATSTGWWTALFSPFATEWTLDNYANVLGRGGMFDAFVNSLIVTIPATVIPITLAA
                     FAAYAFAWIKFRGRAFLFAVVVGLLVVPLQMSLVPLLRIYQATEMTGTFLGIWLAHTG
                     FGLPLAIYLLYNYISQLPKDIFESAYIDGATPFTAFTRLVLPLSYPALAAFAIFQFLW
                     VWNDLLVALVFLGTTSDVSVLTIQLATLAGTRGEAIHLLTAAAFVSMFLPVVVFLLLQ
                     RFFVRGLLAGSVKG"
     gene            complement(1209..2330)
                     /locus_tag="AC161X_05575"
     CDS             complement(1209..2330)
                     /locus_tag="AC161X_05575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015132975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR4989055.1"
                     /translation="MAEVVDPQQVVVERRGWTAGRDFLIAVAVFAILWLGLRLLRNPD
                     TFAGMGPLAGKLAQALLALALGIGGIWAVFIVVNTLVEHLPVRVAEFVRPWVFVLPAL
                     FVVGVYLVYPAIRTIIQSLTEVPEGEGFLANYIDAFTDPDVQIALRNNVIWLLIAPTA
                     SVIIGLAFAALADRVKRESLAKTFVFMPLAISMVGASVIWGFMYAWKIPGQPQVGLVN
                     AVIQGLGGEPIAILQQVPLNTFAMIVIMIWLQVGFAMVILSAAIKGVPVELTEAARID
                     GASELQNFFKILVPSIRGSIITVFTTIAIAVLKVFDIVFVLTGGNLDTEVIALRMYDE
                     MFRFRNFGQATAMAVILFVAVVPIMIINVRNLRKQGIGS"
     gene            complement(2396..3841)
                     /locus_tag="AC161X_05580"
     CDS             complement(2396..3841)
                     /locus_tag="AC161X_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012188735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4989056.1"
                     /translation="MKKRKWSIALVALAMLLAACAESSGGSTTTAGGTEGSTTTAAPS
                     EGSTTTAAGSVGATPYENLNAAMAGDYAGTTVEIVAQWAPDGAEAESFDAALQPFRDA
                     TGIDVQWNPTVEYETALQVRVDGGNPPDLAQIAQPGKMAQYAAAGSLVNLSDWFNVDQ
                     LTQDLCCGFVDMGSVDGSLYGVFWKTDVKSIVWYPIQAFADNGYEPPTTWDEMIALSD
                     QIVADGNGSPWCISIESEAADGWVATDWMEDILLRTAPLETYTAWYNHDIAFNDPAVL
                     HAAELMHQIWFTEGYAYGGSDYINATFIGNTQDPMFDAAGPQCWMQKQAAWIPGFWGE
                     NKDFTDPAEWPNQPGEDVGFFYFPPIDEAYGNPVLGGADMFVMFNDRPEVRALLEYLA
                     TPESAAPWIERGGFLAANKNVPVESYTYPNDELATMTANATSFGFDASDLMPADVGAG
                     TFWSGMVDWVAANGEGTEAVFQGIEDSWPAG"
     gene            complement(3838..4800)
                     /locus_tag="AC161X_05585"
     CDS             complement(3838..4800)
                     /locus_tag="AC161X_05585"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR4989057.1"
                     /translation="MTSTIGVDVGGTSTEGVIVDVQGAVLASSRVTTRTPGAEAVLGS
                     IEAAIERLDWDLPIDGIGVGMPGQVDNTAGTVRLAVNLGIDDAPFPLADRLSTEHGLP
                     VVVENDVRAAAFGVFEKLRQLGDEPGDLALLSLGTGVAAGVVIDGSIHRGATGMAGEI
                     GHVVVDRSGSLCRCGQTGCLETVVSGPAISAAWKAGGEQTPASSLFAAAAAGDQTASA
                     LTQEVTSHLATAIHWLFAAYDVDTIVLGGGLAEAGQPLLDAVRYALVRTAGSSNLATR
                     TLDPKRIRLSPPGGPHGARGAALLAARTFDLALATESNPTGGEI"
     gene            complement(4797..5984)
                     /locus_tag="AC161X_05590"
     CDS             complement(4797..5984)
                     /locus_tag="AC161X_05590"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR4989058.1"
                     /translation="MPELESHRGQPERPSRPKATKHDSRRSNLQTTLDLVSERRATSR
                     AELSRLTGLTRAAVSSLVSELIDDGLLREMGQGTSAGGKPPTLLALNAGGRDIVVVDL
                     GHRPFQGALVDLSGHIRLRLDSPETDVTGQNAVDVAIDLIGLLLEQAEAPILGIGVGT
                     PGVVDAGGMVLEAANLDWHRLDLGKAISSRYGVPVSIANDAQVAALAELRRHPGAGED
                     VLLVKLGRGVGAGVVLGSRLHRGETSAAGEIGHIQVVVDGAPCTCGNSGCLETVASVP
                     AILRSVGADPDRDPWDALALAPVFGEEPLRTALREAGHHLAGVLAHATALLGITRIII
                     APEIRNAGEELIDSVRQGLSGRVLPATEGLIEVELSTLGGDLVLAGAAAAVLTDRLGV
                     VLR"
     gene            6086..6514
                     /locus_tag="AC161X_05595"
     CDS             6086..6514
                     /locus_tag="AC161X_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006901680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR4989059.1"
                     /translation="MRDELVRRRSRIHRALYRMTGGIVGRRLVDNDMLLLTTIGRHSG
                     QPHTVPLLYLRNGEGWVVIASYGGRDRHPAWYLNLVDAPSVEVQISRHHSRARARTAN
                     DEERAHWWPLVVEAYGDYAVYQARTDRVIPVVLLEPMSVG"
     gene            6524..6838
                     /locus_tag="AC161X_05600"
     CDS             6524..6838
                     /locus_tag="AC161X_05600"
                     /inference="COORDINATES: protein motif:HMM:NF016854.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HIR4989060.1"
                     /translation="MAYSEDLVAQVRAQLAPLDDVIEKNVMSGVGFFVDDKMAVAVLE
                     AGLCLRFDQNGTDPAMSPMVSLEFAGRQIQGWVCIPESSLDRASLSRWVSHGVAGLEL
                     PV"
     gene            complement(6827..7852)
                     /locus_tag="AC161X_05605"
     CDS             complement(6827..7852)
                     /locus_tag="AC161X_05605"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: protein motif:HMM:NF004976.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HIR4989061.1"
                     /translation="MPTMKIRVGVLGAGTVGGALVQRLLLDRDLIRQKAGVDLELKLV
                     AVKHLDKPRSFDLPADRITDQAMSVVSSPGIDVVVEAIGGLDPTGDLLLTALRSAKPV
                     VTANKELIATRGEELFEAANRSGVPLMYEAAVGGGIPVIRPLAESLAGERIDRVSGIV
                     NGTTNYILTAMTQDGRSFDEALEEAKVLGYAEPDPSDDVSGRDAAFKTAILARLAFGE
                     WVAPWQVECQGIEELSRADVEAAAAAGQVIKLIGVAERVGDTVSTRVAPVALDAEHPL
                     ASIDGVTNAIYVEGPAIGRLLFTGPGAGGQPTASALLGDLIQVASRRNWQGKAPSYRR
                     PRQVLHR"
     gene            complement(8101..8547)
                     /locus_tag="AC161X_05610"
     CDS             complement(8101..8547)
                     /locus_tag="AC161X_05610"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4989062.1"
                     /translation="MSELSVSREVSVSPAALWELITDLDRSPNVISAVTSIERLDSGM
                     GFGIGTKWRETRLSFGREATEDLEVTHMDPGRSYTVETNGGYRSVISVDPAPAGSVIT
                     ISLGTEPAGPVSKVFAGTIGKLFEGGTRQALVQDLDDIAGAAEANQ"
     regulatory      8665..8751
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
BASE COUNT         1648 a         2700 c         2850 g         1601 t
ORIGIN      
        1 tgcaggtgca catctggccg agtcggattc tgctggtgac ggcggcaagt gccgccatca
       61 gggaccaagc ctcgtaggtc tcctcttggg tggcgatcgg gacggtgtgg aaatggtcgt
      121 agacccacgc ggactcgaaa ccggcggtct ccaatgtcat cgcaacgccg ctcatcgtcg
      181 cccattgctg ttcccggtca atgccggcga gatcgagacg ccaaccctgg gggacgaatg
      241 ctccgaatct catgggcagg gacgatagaa cgtcagcccg cttcgcccga cctgacgggc
      301 gcacggtccc ctcgctccgc agaggtacaa cctgctccgc tatcccttca ccgagccggc
      361 aaggaggcct cggacgaaga accgctgcaa cagcaagaag acgacgaccg gaaggaacat
      421 cgagacaaat gcagctgcag ttagcaaatg gatcgcctca cccctggtac cggcgagggt
      481 tgccagttgg atcgtgagaa ccgagacatc ggaggtggtt ccgaggaaca ccagagccac
      541 cagcaggtca ttccagaccc agaggaattg gaagatcgca aaggcggcca gggccggata
      601 agagaggggc aataccagcc gggtgaatgc ggtgaacggg gtggcgccgt cgatgtatgc
      661 ggactcgaag atgtcttttg gaagttggga gatgtagttg tagagaaggt agatggcgag
      721 cgggagcccg aacccggtgt gagccagcca gatcccaagg aaggtcccgg tcatctcagt
      781 cgcttggtaa atgcggagca acggcacgag tgacatttgg agcgggacca cgagcagccc
      841 gacgacgact gcaaagagga aggcccggcc tcggaacttg atccaggcaa aggcgtaggc
      901 ggcgaaggcg gcgagggtga tcgggatgac ggtcgccggg atggtcacga tcaagctgtt
      961 gacaaatgcg tcgaacatcc cgcctctgcc caggacgttg gcgtagttgt cgagtgtcca
     1021 ttcggtggcg aacgggctga acagggccgt ccaccaaccg gtgctggttg catctgaagc
     1081 agggcggaag gagctgacca gaagcccgac ggttgggatg gtccacagga agacgatcag
     1141 gaacaccgaa agcttgactg cccacccgct cttggtggtc gtgcccgggg ccttgcttgc
     1201 caccgcggtc atgatccgat gccttgcttc cggaggttgc ggacgttgat gatcatgatc
     1261 ggaaccaccg caacgaacag gatcacggcc atcgccgtcg cctggccgaa gttgcggaat
     1321 cggaacattt cgtcgtacat cctcagggcg atgacctcgg tatcgaggtt gcccccggtt
     1381 aggacaaaga cgatgtcgaa caccttgagc accgcgatgg cgatcgtggt gaacaccgtg
     1441 atgatcgagc cccgaatcga aggcaccagg atcttgaaga aattctgaag ctcagaggcg
     1501 ccgtcgatgc gggcggcttc ggtcaactcg acgggtacgc ccttgatcgc cgctgagaga
     1561 atgaccatgg cgaatccgac ctgaagccag atcatgatca cgatcattgc gaaggtgttc
     1621 aaggggacct gctggaggat ggctatgggc tcgcctccca gcccttgaat gacggcgttg
     1681 accaagccga cctggggctg gccggggatc ttccatgcgt acatgaagcc ccagatcaca
     1741 gaggctccga ccatggatat cgccagaggc atgaaaacga aggtcttcgc cagggactcg
     1801 cgtttgactc ggtcggcaag agccgcaaac gccagcccga tgatcacgga ggccgttgga
     1861 gcgatcaaca accaaatgac gttgttgcgc aaagcgattt gcacatcggg atccgtgaaa
     1921 gcatcgatgt agttggccag gaaaccctcg ccttcgggta cctcggtcaa gctctggatg
     1981 atggtgcgga tggccgggta gaccagatag acgccaacca cgaagagggc agggagaacg
     2041 aacacccagg gtcgcacgaa ttcggcgact cgaaccggga ggtgctcgac aagggtgttg
     2101 acaacgatga acaccgccca gatcccgccg atgcccaggg caagggccag gagtgcttgg
     2161 gcaagcttgc cggcgagtgg gcccatgccg gcgaaggtgt ccgggttgcg gaggagtcgc
     2221 aagccaagcc agaggatggc gaacacggcg acagcgatca ggaagtcgcg cccagcggtc
     2281 cagccgcggc gctccacgac tacctgctgc gggtcgacga cttcggccaa agtgccctcc
     2341 tacaaaagga aaaggtcccc ggggttaccc ccggggacct taccaactcg ggaggtcagc
     2401 cggccggcca gctgtcctcg attccctgga ataccgcctc ggtgccttca ccgttggcgg
     2461 ccacccagtc gaccatcccc gaccagaagg tgccggcgcc gacgtcggcg ggcatcaggt
     2521 cagaggcatc gaagccgaag gaggttgcat tcgccgtcat tgttgccaac tcatcgttcg
     2581 ggtacgtgta ggactcgacc ggaacgttct tgttggcggc gaggaagcca ccacgctcga
     2641 tccacggagc cgcggactcg ggggtggcca ggtattcgag cagtgcccga acctctggcc
     2701 gatcgttgaa catgacgaac atgtcagcgc cgcccaacac cgggtttcca taggcctcgt
     2761 cgatcggcgg gaagtagaag aacccaacgt cctcacctgg ctggtttggc cattcggcgg
     2821 ggtcggtgaa gtccttgttc tctccccaga accctgggat ccatgctgct tgcttctgca
     2881 tccagcactg cggcccggcc gcgtcgaaca tcgggtcctg agtgttgcca atgaaggtcg
     2941 cgttgatgta atcgctgcca ccgtaggcgt acccctcggt gaaccagatc tggtgcatga
     3001 gctcggcggc atggagcacc gcaggatcgt tgaacgcgat gtcgtggttg taccaggcgg
     3061 tgtaggtctc gagcggcgca gtgcgcagca ggatgtcctc catccagtcg gtagcgaccc
     3121 agccgtcggc tgcttcgctc tcgatcgaga tgcaccatgg gcttccattg ccatcagcga
     3181 cgatctgatc actcagtgcg atcatctcgt cccaggtggt gggcggctcg tagccgttat
     3241 cggcgaaggc ctgaatcggg taccagacga tcgacttgac gtcggtcttc cagaacacgc
     3301 cgtaaaggct tccatccacc gaccccatgt cgacgaaacc acagcagaga tcctgtgtta
     3361 gctggtcgac attgaaccag tcgctcaggt tgaccagcga gccggctgcg gcgtattgcg
     3421 ccatcttccc cggctgggcg atctgggcca ggtccggtgg attgccgcca tcgacccgca
     3481 cttggagcgc ggtttcgtac tccacggtcg ggttccactg aacgtcgatc ccggtggcgt
     3541 cccggaacgg ctgaagggca gcgtcaaaag actccgcctc ggccccgtcc ggcgcccact
     3601 gggcgacgat ctcaaccgtg gtgcctgcat agtcgccggc catcgcggca ttcaggttct
     3661 cgtagggggt cgcaccaacc gagccagcgg ctgtcgtggt cgacccttca ctgggagcgg
     3721 ccgtagtcgt ggacccctcg gtcccaccgg ccgtcgttgt ggaaccgcca ctcgactcgg
     3781 cgcatgctgc gagcagcatg gccagagcca cgagagcgat cgaccacttc cgtttcttca
     3841 tatttctcct cctgttgggt tgctttcggt cgcgagggca agatcgaatg tgcgggcggc
     3901 cagtaaggcc gctccacggg cgccatgagg gccaccgggc gggctgagcc tgatccgttt
     3961 cggatcgaga gttcgtgttg caaggttgga ggatcctgcg gtcctcacca gagcgtaacg
     4021 gacagcgtcc agtagcggct ggccggcctc agccagtccc ccgcccagga cgatggtgtc
     4081 gacgtcgtat gcggcgaaga gccaatggat ggctgtggcg aggtgggagg tgacctcctg
     4141 ggtgagtgcc gatgctgtct ggtctcctgc cgcggcggct gcgaagaggg atgaggccgg
     4201 tgtttgctcg ccgccggctt tccatgccgc cgagatggca ggcccgctca ccaccgtttc
     4261 caggcatccg gtctgaccgc agcgacaaag tgagccgctt cgatcaacaa cgacatgccc
     4321 gatctcgccg gccatcccgg tggcgccgcg gtggatcgat ccatcgatga ccaccccggc
     4381 ggcgacgccg gtgccaaggc tgagaagtgc gaggtctccg ggctcgtctc ccaattggcg
     4441 cagcttctcg aagacgccga atgctgccgc cctcacgtcg ttctcgacca ccaccggcaa
     4501 cccatgctcc gtagaaagcc tgtcggccaa tgggaagggg gcgtcgtcga tgccgagatt
     4561 gactgcgagt cgcacggtgc ctgccgtgtt gtccacctgc cctggcatgc ccaccccgat
     4621 cccgtcgatc ggaaggtccc aatcgaggcg ttcgattgcc gcttcgatcg agccgaggac
     4681 agcttctgct cccggggtcc gggtcgtcac ccgtgagctg gccaggactg ctccttgaac
     4741 atcgacgatg accccctctg tggatgtccc gccgacgtcg actccgatgg tgctggtcat
     4801 cgcaacacca cgccgaggcg atcagtaagt actgcggctg cggcgccggc aaggaccaga
     4861 tccccgccca gtgtcgatag ctcgacctcg atcaggcctt cagttgcagg caacacgcga
     4921 ccactgaggc cttggcgcac actgtcgatc aactcctcac cggcgttccg gatctcgggg
     4981 gcgatgatga ttcgggtgat gccgagcagg gcagtggcgt gagcaagcac accggcaagg
     5041 tggtgtcctg cctcccgcaa tgcagtgcgc agtggctcct cgccgaacac gggagctaag
     5101 gcgagagcat cccacgggtc gcgatcgggg tccgctccca cgcttcgaag gatcgccggg
     5161 acactggcga cagtctccag gcagccggag ttaccgcagg tgcatggtgc gccgtccacc
     5221 actacctgga tgtgcccgat ctccccggca gcagacgttt cgccacgatg aaggcgagag
     5281 ccgagcacga cgcctgcgcc gacgccccgg cccaacttga cgagcagcac atcctctcca
     5341 gcgccggggt gtctccgaag ctcggcaagc gccgccacct gggcgtcatt ggcgatggaa
     5401 accgggaccc cgtatcggga ggagattgcc tttccgaggt cgagacgatg ccaatccagg
     5461 ttcgcggcct ccagcaccat gcccccggcg tcgaccacac caggggtgcc gacaccgatg
     5521 cccaggattg gggcctctgc ctgctcgaga agcaagccga tcaggtcgat ggcgacgtcc
     5581 acggcgtttt gaccggtcac gtcggtttcg ggggagtcga gccgtagccg gatgtgcccg
     5641 ctgaggtcga caagagcgcc ctggaatggc cggtggccga gatcgacgac gacgatgtcg
     5701 cgacccccgg cattgagtgc gagcagggtc ggtggcttcc caccggcaga cgtgccttgg
     5761 cccatctcgc ggaggagacc atcgtcgatc agctcgctca cgaggctgga aacggccgct
     5821 ctggttaggc cggtcagccg ggacaattcg gcgcgggagg tggcccggcg ctcggacacg
     5881 agatcgagag tggtctgcag gttggatcgg cgcgagtcgt gcttggtggc cttggggcgg
     5941 ctgggacgtt cgggttggcc ccggtgggac tcaagttcag gcatgaaagt tcactgaaca
     6001 tacaaactcg gaaggggcgg tgtcaagcga ctttggggtc ggaatatcgt gtggcggaat
     6061 ccttgatcga ttggagtggt cgagcgtgag ggacgaacta gtgcgccgca ggtcccgtat
     6121 ccaccgcgcc ctataccgaa tgaccggcgg gatagtcggg cgccggttgg ttgacaacga
     6181 catgctgctg ctcaccacga tcggccggca ctctggtcag ccccacaccg tgcctctcct
     6241 ctatttgcgg aatggagagg gctgggtcgt cattgcctcc tacgggggac gggaccgaca
     6301 ccctgcctgg tacctcaatc tggtcgatgc gccgtcggta gaggttcaga tctcgagaca
     6361 tcactcacgc gctcgggccc ggactgcaaa tgacgaggag cgggctcatt ggtggccact
     6421 ggtcgtggag gcgtatggcg actacgccgt ctaccaagcc cgaaccgacc gtgtcatccc
     6481 ggtggtctta ctggaaccga tgagcgttgg atagattcgc gcggtggcct actcagagga
     6541 cctcgtggca caggtccgtg ctcagcttgc acccctcgat gatgtcattg agaagaacgt
     6601 gatgtcaggg gttggcttct tcgtggacga caagatggcg gtcgcggtcc tcgaagccgg
     6661 cctgtgtctt cgctttgatc aaaacgggac cgacccggcg atgagtccga tggtgtccct
     6721 ggaattcgcc ggccgccaga ttcaagggtg ggtttgcata ccggagtcga gcctcgaccg
     6781 ggccagtctt tcacgatggg tcagccacgg cgtcgccggg ctggaactac cggtgtagga
     6841 cctggcgagg gcgccgatag ctaggagcct tgccctgcca gttgcgccgg gatgccactt
     6901 ggatcaagtc accgaggagg gccgacgctg tgggctggcc accggctccc gggccggtga
     6961 agagaagtcg cccgatggcg ggcccctcca catatatggc gttggtgaca ccatcgatgc
     7021 tggccagcgg gtgctcggcg tcgagcgcga ccggggcaac tcgtgtcgac accgtgtctc
     7081 caactctctc ggcgacgccg atgagcttta tcacctgtcc cgccgcagcg gcggcctcga
     7141 cgtccgcccg gctcagctct tcgattccct ggcactccac ctgccacggt gcgacccatt
     7201 cgccgaacgc gagtcgggcc aggatcgcgg tcttgaacgc agcatccctt ccggagacgt
     7261 cgtcggatgg gtcgggctcc gcgtagccga ggaccttggc ttcctcgagc gcctcgtcga
     7321 agctgcgacc gtcctgggtc attgcggtca ggatgtagtt cgttgtcccg ttgacaatcc
     7381 cactgacgcg atcgattcgc tcgccggcga ggctctcggc aagtggccga atcaccggga
     7441 ttcccccgcc gactgccgcc tcatacatga ggggcacgcc gctccgattt gctgcctcga
     7501 aaagctcctc gcccctggtg gcgatcagtt ccttgttggc ggtcacaacc ggcttcgctg
     7561 acctcagggc cgtgagcagc aggtccccgg tcgggtcgag accaccaatg gcctcgacca
     7621 ccacgtcgat gcctgggctg ctgacgacac tcattgcctg gtcggtgatc cggtccgcgg
     7681 ggagatcgaa ggatctgggc ttgtccagat gcttcacggc gacaagcttc aattcgaggt
     7741 cgacgcccgc cttctgccga atcagatccc ggtcgaggag gagtctctgc accagggcgc
     7801 ccccgacggt gccggcgccg agcactccaa ctctgatctt catcgtcggc attcttggtt
     7861 cctcgatccc gggtcgtgtc tcattcggtt cctctggtgg ttgagaatcc cgacaccggc
     7921 ctgaatcaaa aagatccgca ccggtgccgg gcggatctcg ttgtcgtgtc ctggtgggtg
     7981 ctgatcacac gaccgagccg ctcggctggc tagccgtcgt catggtcgta gtgatgcaaa
     8041 cagcactcat gtcatggtca aggtatccga ccctgggcta tacgtcaacg cgtagcagct
     8101 tcattgattg gcctcggcgg cacccgcgat gtcatcgaga tcctggacga gggcctgacg
     8161 agtgccccct tcgaagagct tgccgatcgt cccggcgaac actttggaga ctgggcctgc
     8221 gggctccgtt cccaacgaaa tggtgatgac gcttccagcg ggtgccggat cgacgctgat
     8281 cacggagcgg taaccgccat tggtctcgac cgtgtaactg cgcccgggat ccatgtgggt
     8341 gacttcgagg tcctcagtgg cctcccggcc gaaactcagc cttgtttctc gccatttagt
     8401 ccctattccg aatcccatcc cggagtcgag gcgctcgatc gaggtgacgg cggatatcac
     8461 gttgggtgag cggtcgagat cggtgatcag ctcccacaat gctgcggggc tgaccgaaac
     8521 ctcgcgactc accgagagct ccgacatggc acgtcctcct ttgtgcctgc aagaacctag
     8581 agcgaaaggc ctgacagggc gggtatcggc gtcgacttcg ctgccacttc aggggaagcc
     8641 ctggttatca tctacgttgc gaagcgctca tctagagcgg ttgagggact aggcccggtg
     8701 ataccgcggc aaccggcgca agcacggtgc caatgcctgg aacgatgagt tgccgtaggg
     8761 ctccggctct cggcgctcac gaggcgtttc gagagacaa
//
LOCUS       DBNHXU010000225         1772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_665458, whole genome shotgun sequence.
ACCESSION   DBNHXU010000225 DBNHXU010000000
VERSION     DBNHXU010000225.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1772
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_665458"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1133
                     /gene="hemW"
                     /locus_tag="AC161X_05615"
     CDS             <1..1133
                     /gene="hemW"
                     /locus_tag="AC161X_05615"
                     /inference="COORDINATES: protein motif:HMM:TIGR00539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051989 - coproporphyrinogen dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM family heme chaperone HemW"
                     /protein_id="HIR4989063.1"
                     /translation="YVHIPFCSSVCPYCDFAVVAGADHLIDSYATALISEIEMSENWE
                     ELDSVYVGGGTPSHVDPEILGRILRALSLRHGLGDGAEISLEANPEDFDDARAAEVAG
                     AGFNRVSFGAQSFDRGVLQALGRRHGPEQIISSVGAARGASIPSVSVDLIFGTPSETD
                     DSWERTLQGAVEAGPDHVSCYALTVEPGTPLNKAVMAGASAPDPDVQADRYEMAEAKL
                     GEAGYHRYEVSNWSRPGHECRYNLTVWAQGQYEAYGNGAHRFRDGVRSHNVRRLEAYI
                     SELEAGRRPIAGAEPVRGWDSEIDRLFVGLRRADGVVDGPGVRSLVTSEEGKELQEAG
                     VIEMNGDRLVVRRPLLTDMVHRAVLGLEAPVGWVEPPTPDNV"
     gene            1136..>1772
                     /locus_tag="AC161X_05620"
     CDS             1136..>1772
                     /locus_tag="AC161X_05620"
                     /inference="COORDINATES: protein motif:HMM:NF013768.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HIR4989064.1"
                     /translation="MLEDRRSEILRALVEEHIRTGEPVSSRVVVEKAHLSVSTATVRN
                     DLAALEQEGLVVQPHTSAGRIPTATAYRYYVDHLGPGRLRSAAQAKVNSLFGDVHLEL
                     SKLLKATSDLLAEITDLPAVVVSPGIVGDRVRAVQSVQVTADQILIVVVTEGGQVLQQ
                     RGRVRVPVTPFDIEEAQRLVSAAVVGSDLGLNLQLGDEVMAQLNEPTREAVL"
BASE COUNT          305 a          552 c          595 g          320 t
ORIGIN      
        1 cctatgtcca catcccgttt tgctcctcgg tttgccccta ttgcgacttt gcagtcgtgg
       61 cgggcgccga tcacctgatc gacagctatg cgacggctct gatttccgag atcgagatgt
      121 ccgagaactg ggaagagctg gactctgtct acgtcggggg cgggaccccc agtcacgttg
      181 atccggagat cctgggtcgg atcctgaggg ccttgtctct ccgtcacggc ctgggcgatg
      241 gggcggagat ctccctcgag gcgaaccccg aggacttcga cgacgcccgg gcagcagaag
      301 tcgccggcgc agggttcaac cgagtctctt tcggagcgca gagcttcgat cggggtgttc
      361 ttcaggcgtt gggccgccgc catgggcccg aacagatcat ctcctcggtc ggcgctgccc
      421 gcggcgcatc gataccgagc gtctcggtcg acttgatctt tggcacgcca tcggagaccg
      481 acgattcatg ggagcggaca ctccagggcg cggtcgaagc cgggccggat catgtctcct
      541 gttacgcatt gaccgtcgag ccggggacgc cactcaacaa agcggtcatg gccggagcgt
      601 ctgccccgga tcctgacgtc caggccgatc ggtatgagat ggccgaggcc aagctgggtg
      661 aagcgggcta ccaccgatac gaggtctcga actggagccg tcccgggcat gagtgccgtt
      721 acaacctgac cgtgtgggcc cagggccagt acgaggcata tggcaacggg gcgcaccggt
      781 ttcgtgatgg cgtgcgcagc cacaacgtcc ggcgtctgga ggcctacatc tcggaactcg
      841 aggcggggag acggcctatt gccggagccg agccggtgcg aggctgggac tccgagatcg
      901 atcggctgtt tgtcggcctc cgccgagccg atggggtcgt cgatgggccg ggggttcggt
      961 cgctggtcac gtcggaggaa ggaaaggagc tccaggaggc cggggttatc gagatgaatg
     1021 gcgaccggct ggtggtgaga aggccgctgc tcaccgacat ggtccaccgt gctgtgctcg
     1081 gcctcgaagc acccgtcgga tgggtggagc caccaacgcc ggataacgtg taggcatgct
     1141 cgaagatcgc cgctcagaga tcctcagggc actcgtggag gagcacatcc gaaccgggga
     1201 gccggtgagc tcgcgcgtcg tcgtggagaa ggctcatctc tcggtctcca ccgccactgt
     1261 tcgcaacgac cttgctgcac tcgagcaaga gggattggtg gtccagcccc acacctcggc
     1321 tggaaggatc cccacggcca ccgcctaccg ctactacgtc gaccatctcg gccccggccg
     1381 ccttcgctcc gctgcccaag cgaaggtcaa cagcctgttc ggcgatgtcc atctcgagct
     1441 ttccaagctg ttgaaggcca cttccgacct tctcgctgag atcaccgacc ttcccgcagt
     1501 cgtcgtctcg ccggggatcg tcggggaccg ggtgagagcg gtgcagagtg tgcaggtcac
     1561 cgccgaccag atcctcatcg tcgtcgtcac cgagggtggc caggtgctcc agcagagggg
     1621 ccgggtcagg gtcccggtta ctcccttcga catcgaagag gcgcagcgtc tcgtgagtgc
     1681 agcggttgtg ggcagcgacc tcggcctcaa tctccaactg ggagacgagg tcatggctca
     1741 gctcaacgaa ccgacccgag aggctgtgct ga
//
LOCUS       DBNHXU010000226         3204 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2849485, whole genome shotgun sequence.
ACCESSION   DBNHXU010000226 DBNHXU010000000
VERSION     DBNHXU010000226.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3204)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3204
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2849485"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..93
                     /locus_tag="AC161X_05625"
     CDS             <1..93
                     /locus_tag="AC161X_05625"
                     /inference="COORDINATES: protein motif:HMM:NF019886.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HIR4989065.1"
                     /translation="ETVATQMGVSPGAVKRHLARARKHLKESLS"
     gene            114..1208
                     /locus_tag="AC161X_05630"
     CDS             114..1208
                     /locus_tag="AC161X_05630"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gmad2 immunoglobulin-like domain-containing
                     protein"
                     /protein_id="HIR4989066.1"
                     /translation="MKDLADRLVAMSPEPPPFPEETTVTQPTNGAKKQSPVLIFAGAA
                     AIVIVLALIPILLLRGGGEVAPIGTTTTSTVPSTQSSEPVVTSTSIAEVTTTLPETTT
                     TAPVAETQGVFVFLVQTPENSNTGNPALVPFFSSAEAVPGDPTLASLRLLTADGLTPP
                     PGFQSMIPVEVEVVGVTRAPSDQLITVDMNQAFVDGAGGLLADFTMLNQLIFTASDGE
                     EISEVLFTVNGDPVTAFGTEGLDISRPLGRDAFLDQLNSVNLDSAPTGTGDAPLVISG
                     FANVFEATVSLELVDGDGNVVYEAFTTATCGTGCWGAFSFNIDDFDFEATPVSVRVFW
                     HSAKDGSPSDVVTVPILWNDEGGWDLITGS"
     gene            1339..1704
                     /locus_tag="AC161X_05635"
     CDS             1339..1704
                     /locus_tag="AC161X_05635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005544817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR4989067.1"
                     /translation="MTPYFESYLTVGAFAALGAILVMVMLGVASVLRPKNPTSAKEMT
                     YECGVDPVGVGWSQTYVRYYVYGLLFIIFDVEAVFIFPWAIILEKLGVFGLVEMLIFI
                     FILVLGLVYAVRKGVLRWE"
     gene            1719..2192
                     /locus_tag="AC161X_05640"
     CDS             1719..2192
                     /locus_tag="AC161X_05640"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015507712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
                     /protein_id="HIR4989068.1"
                     /translation="MPKPVTWLLNWSRKYSLWLFQFGLACCAIELMAASGPRYDFMRF
                     GIIPLPASPRQADLMVVAGTVTDKMAPAVRRLYDQMPEPKYVISMGSCSNCGGPYWDS
                     YSVTKGVDQIIPVDVYVPGCPPRPEALMDGIVLLQKKIQGENIKEKWNQRDLEPV"
     gene            2173..2778
                     /locus_tag="AC161X_05645"
     CDS             2173..2778
                     /locus_tag="AC161X_05645"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012549.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR4989069.1"
                     /translation="MTSSPSETETAVHPLQAFADRVAEVVGGVAEITFDTVKVKVPVE
                     RWVEIHRQARDDLGLVFFSWLSAVDWTLDVAVGDPPGGEVIDRFEMLTTVGDVSEGKR
                     VTFTADLSKESPVIDSLVEVYAGANWHEREAAEMFGIRFEGHPDLSHLYLVDSFIGNP
                     LRKSYPLLSREVKPWPGTVDVEGMPGSAAEPDEPSEENPEA"
     gene            2780..>3204
                     /locus_tag="AC161X_05650"
     CDS             2780..>3204
                     /locus_tag="AC161X_05650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit D"
                     /protein_id="HIR4989070.1"
                     /translation="MVADQDLVRHLSEASVSVSLETPEMTLNIGPQHPSTHGVLRLVA
                     RVDGERAYDVHPVIGYMHRGYEKLSEVRTYPQITTLVNRIDWVSGYANEVPFIVAAEK
                     LMQVEVPERAQYIRLILTEMARISSHLIFMASYPLELGAA"
BASE COUNT          589 a          995 c          985 g          635 t
ORIGIN      
        1 gaaacagtcg ctacccaaat gggtgtctcc ccgggcgcgg taaagcgtca tctcgcccga
       61 gccaggaagc acctcaagga gtcgctgtca tgagcggtat cgacgaccag agaatgaaag
      121 atcttgccga ccggctggtg gcgatgtccc cggagccacc gcccttcccc gaggagacca
      181 ctgtgaccca accaaccaac ggcgcgaaga agcagagccc tgtcctgatc ttcgccggag
      241 ccgcggcaat cgtgatcgtc cttgccctga tcccgatcct tcttcttcgt ggaggcggtg
      301 aggtggcgcc gatcgggacc accacgacat cgaccgtgcc ctcaacccaa tcgagcgagc
      361 cggtggtgac gtcgacatcg attgccgagg tcaccacgac gttgccggag accacgacaa
      421 ccgccccggt cgcggagacc caaggggtgt tcgtgtttct cgtccaaaca ccggagaact
      481 cgaacacggg caatccggcg ttggtcccat tcttctcctc agcagaggcc gtgccgggcg
      541 atccgacgct agcttcgctg cggctcctaa ccgccgatgg cctgaccccg cctccgggat
      601 tccagtccat gatccccgtc gaggtcgagg tcgtgggggt gacccgggcc ccgagtgacc
      661 agctgatcac agtcgacatg aaccaggcgt ttgtcgatgg cgccggaggg ctgctcgccg
      721 acttcaccat gctcaaccag ctgatcttca ctgccagtga tggagaggag atctccgagg
      781 tgttgtttac ggttaacgga gatccggtca ctgcgtttgg aaccgaaggt ctcgacatca
      841 gccggcccct tggccgagat gcgttcctcg accagctcaa ctcggtgaac ctcgattcag
      901 cccctaccgg aactggtgac gcgccgttgg tgatctccgg gttcgccaac gttttcgaag
      961 ccactgtgag cctggagctg gtcgatggcg acggaaacgt cgtgtacgag gctttcacca
     1021 cggccacgtg cggaaccggg tgctggggag cattcagctt caacatcgac gacttcgact
     1081 tcgaggccac accggtgtcg gtccgggtgt tctggcattc cgccaaggac ggttcgccca
     1141 gcgacgtggt gaccgttccc atcctgtgga acgacgaagg cggctgggac ctgatcaccg
     1201 gcagctgaag tcctctgccc agaaaggggg tttcgacgag gcccatcgcc cgtcgaaacc
     1261 cccttttccg tctgacttcg gggttcaagc caccctgcgt atactcgccg tcggtcgcaa
     1321 tcacgacgga aaagggcaat gactccgtac ttcgagagct atctgacggt cggcgccttc
     1381 gccgctctgg gagcgatcct cgtgatggtg atgctcggtg tcgcatcggt cctgcgaccc
     1441 aagaacccca cctccgcaaa agagatgacc tatgagtgcg gggttgaccc ggtcggcgtc
     1501 ggatggagcc agacctacgt ccgctattac gtctacggcc tgctcttcat catcttcgac
     1561 gtcgaggcgg ttttcatctt tccctgggcg atcatcctgg agaagctcgg ggtcttcggc
     1621 ctggtcgaga tgctgatctt catattcatc ctggtcctgg ggctggtcta cgcggtccgc
     1681 aagggagttc tgcggtggga gtagcccaga agttcggcat gccaaagccg gtcacctggc
     1741 tgctgaactg gtcgaggaag tactcccttt ggctgttcca gttcgggctg gcctgttgtg
     1801 caatcgagct gatggcggct tccgggcccc gatacgactt catgcgcttc gggatcatcc
     1861 cccttcccgc ctctccgaga caggcagacc tgatggtggt ggcaggcacc gtgaccgaca
     1921 agatggcgcc ggccgtgcga cgtctttacg accaaatgcc tgagccgaag tacgtgatct
     1981 caatgggctc gtgctccaac tgtggtggcc cctactggga ctcctactcg gtgaccaagg
     2041 gtgtcgacca gatcatcccg gtcgatgtct atgtcccggg ctgccctccg cggccggagg
     2101 cgttgatgga cgggatcgtt cttctccaga agaagatcca gggagagaac atcaaagaga
     2161 agtggaacca gcgtgacctc gagcccgtct gagaccgaga ccgcagtcca tccccttcaa
     2221 gcattcgccg accgggtggc ggaggtggtc ggaggcgtag ccgagatcac ctttgacacc
     2281 gtcaaggtga aagtccccgt tgaacgctgg gtggagatcc atcgccaggc ccgggacgat
     2341 ctcgggcttg ttttcttctc gtggctgtcc gctgtcgact ggacactcga tgtggccgtc
     2401 ggcgaccccc ccggtggtga ggtcatcgat cgattcgaga tgctgaccac tgtcggcgac
     2461 gtcagcgagg ggaagcgggt gacgttcacc gccgatctct ccaaggagag cccggtgatc
     2521 gacagcctgg tcgaggtcta cgcgggcgcc aactggcatg agcgggaagc ggcggagatg
     2581 ttcgggatcc gcttcgaagg ccatcccgat ttgagccatc tctatctcgt ggattcattc
     2641 atcggcaacc cgctccgcaa gagctatccg ttgctcagcc gcgaggtgaa gccctggccc
     2701 ggaacggttg atgtcgaggg catgcccggg tctgcggccg agcccgacga accgtcagaa
     2761 gagaacccgg aggcctgacg tggttgccga tcaggatctg gtccggcacc tttccgaggc
     2821 gtcggtttcg gtctcactgg agaccccgga gatgaccctc aacatcgggc cgcagcaccc
     2881 ttcgacccat ggggtgctcc gtttggtggc ccgggtagat ggggaacgtg cctatgacgt
     2941 gcaccccgtc atcgggtaca tgcatcgtgg ttacgagaag ctgtccgagg tccgtacgta
     3001 tccccagatc accaccttgg tcaaccgcat cgactgggtc tccggatacg ccaacgaggt
     3061 gcccttcatc gtcgctgccg agaagttgat gcaagtggaa gtcccggagc gagcccaata
     3121 catccggctg atcctcaccg agatggcccg catctcctca cacctgatat tcatggcgtc
     3181 atatccgttg gagttgggcg ccgc
//
LOCUS       DBNHXU010000227         2992 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_666257, whole genome shotgun sequence.
ACCESSION   DBNHXU010000227 DBNHXU010000000
VERSION     DBNHXU010000227.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2992)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2992
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_666257"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            217..1188
                     /locus_tag="AC161X_05655"
     CDS             217..1188
                     /locus_tag="AC161X_05655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007729679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4989071.1"
                     /translation="MRRIALLVALLLAVGACGTAGSGDTTTSQASATSVVETTTTLNT
                     TTTQSDGFPVSVTDGNGEVTIESQPQRIVSLSSTATEMLFAIDAGDQVVAVDEFSVYP
                     EEAPITDLSGFTPDVEAILEYEPDLVVISYDPGELVSGLSAVGVPTLLLSTAGSLDDS
                     YAQIEILGASTGHVGEAAEVVSQMQVDIDGILAATTVPPGLSFYHEVDSTLYSASSAS
                     FIGQVYALLGMENIADEADPDGLGFPQLASEYVVSADPDLIFLADSSMGVTPESLADR
                     PGWAEMTAVQGGAVTVLDEYLASNWGPRVVELLQSIADSVTEMVPAG"
     gene            1227..2228
                     /locus_tag="AC161X_05660"
     CDS             1227..2228
                     /locus_tag="AC161X_05660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010542258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR4989072.1"
                     /translation="MGRARWLAAIAILVVVGMAGILVGSVALDPASVLRALADRLPFV
                     SVASGLTEVQERVLFEIRLPRVVMGGMVGSVLALAGAGYQGVFRNPLADPYLLGVAAG
                     AGLGATFVIGAGGASALIPPFAFVGGLVAVLATYAVGRTAGGRTITSLILAGVAVAAF
                     ATAIQTFLLQRNYETLREVYSWILGRLVTVGWGEVLTLLPYALIAGVVLVLYRRLLDV
                     LALGDEEASGLGVDVVKVRRVVVLAATLATAAAVSVSGLIGFVGIIVPHTVRLLTGWS
                     YRLIIPLSILYGAAFLILADLIARTALAPAELPIGVVTAFLGAPFFIVVLRSVRGSQ"
     gene            2228..2980
                     /locus_tag="AC161X_05665"
     CDS             2228..2980
                     /locus_tag="AC161X_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989073.1"
                     /translation="MAWHEVAVTFGGPPVVGPLSLTVVDGEWLALIGPNGAGKSTLLK
                     AAVGIVEHSGSVQFGGRASRAGLDVAWMPQRPYLPEEMSVTDYVLLGRTPHLGRFGTE
                     READLDSAQRSIQRLELGALADRALATLSGGEAQRAVLARAIAQEAPVLLLDEPTSNL
                     DIGHSLDVLEIVDDLRLSDGLTVVTALHDLTLAGRFAGRVVMLASGKVVADGSPDEVL
                     TEELLRSHYGAGIRVVDNGEGPTVIPTRNRRV"
BASE COUNT          473 a          909 c          989 g          621 t
ORIGIN      
        1 taccagtgcc gggcccgctt ccttttggat gggggcaccc ggaggacagc gaagccggtt
       61 tgatcccggt gctgtcccgc aactgtgaag cccccatcgg gggacgagcc aggtcgcctg
      121 aacctccggg tcacgcaata agtctcggat gaagacgatt cgtgggctca gggaagccac
      181 cggcctcttc atcctccaac cggaggatgt tttcatatgc gaaggattgc gctattggta
      241 gcgctcttac tggcggtggg agcctgcggc accgccggaa gtggtgacac caccacgagc
      301 caggcatctg cgacctcggt ggtcgagacg acgaccacgc tgaacaccac aaccacacag
      361 tcggacggtt tcccggtgtc ggtcaccgac ggaaacggcg aggtgaccat cgagagccag
      421 ccgcaaagaa tcgtgtcgct gtcctcgacg gcaactgaga tgctgtttgc gatcgatgcc
      481 ggggaccagg tggtggcggt tgatgaattc tcggtatatc ccgaggaggc gcccatcacc
      541 gatctctccg ggttcacccc cgatgtcgag gcgatcctcg agtatgagcc cgacctggtg
      601 gttatctcct acgaccctgg cgaactggtc tcaggcctat cggcggttgg ggttcccaca
      661 ttgctcttgt cgacggcggg gagtctcgac gattcctacg cccagatcga gattctcggt
      721 gcatcgaccg ggcacgttgg tgaggccgca gaggtggtga gtcagatgca agtcgacatc
      781 gacggcatct tggcggcgac caccgttccg cccgggctca gcttctatca cgaagtcgac
      841 agcacgcttt actcggcatc ttcagccagc ttcatcggcc aggtctacgc cctcctcggg
      901 atggagaaca ttgccgatga ggccgatccc gacgggctcg gattccctca gctggcgtcg
      961 gaatacgtgg tctcggcgga tcctgacctg atcttcctcg cggattcgag catgggagtg
     1021 accccggaga gccttgctga tcgtcccggg tgggccgaga tgacggcggt gcagggtggt
     1081 gcggtcacgg tcctcgatga gtatctcgct tcgaactggg gacctcgggt cgtcgaactg
     1141 ttgcagtcga ttgctgactc ggtgacggag atggtccccg ccgggtgagc caggaagcga
     1201 caggctccgc tacccgccca cctcgaatcg ggcgtgcccg atggctggcg gcgatcgcga
     1261 tccttgtcgt tgtgggaatg gccgggattc tggtgggatc ggttgcactc gatcccgcca
     1321 gtgtcctcag agcactggcc gatcggctcc ctttcgtcag tgtcgccagt ggtctgaccg
     1381 aggttcagga gcgggttctg ttcgagatcc ggctgccgag ggtggtgatg ggaggaatgg
     1441 tcggctcggt gttggcgttg gccggggccg gctatcaagg ggtgtttcgc aacccattgg
     1501 cagacccgta tctccttggc gtggctgcag gagccggtct cggggctacc ttcgtgatcg
     1561 gggccggcgg agcatcggcc ctgataccac cgttcgcctt cgttggcggc ttggtggcgg
     1621 tactggcgac ctacgcggtc ggccgcactg ccggcgggcg aacgatcacc tcattgatcc
     1681 tggccggggt tgcagtcgct gcattcgcca ctgcgatcca gacctttctg ctccagcgca
     1741 actacgagac cctgcgcgag gtctactcgt ggatcctcgg ccggctggtg accgtggggt
     1801 ggggcgaggt tttgactctt cttccgtatg cgctgatcgc cggtgtggtg ctcgtcctct
     1861 accggcgtct cctcgacgtg ctcgccctgg gcgatgagga ggcctcgggg ctcggagtcg
     1921 acgtggtcaa agttcggcga gtcgtagtcc tggctgcgac gttggccacc gcggccgcgg
     1981 tctccgtcag cgggctgatc ggcttcgtcg ggatcatcgt ccctcacacg gtgcggctgc
     2041 tgaccggttg gagctatcga ctgatcattc ccctttccat cctttacggg gcagccttcc
     2101 tgatccttgc tgacctcatc gcccgaactg cactggctcc cgctgaattg ccaatcggtg
     2161 tcgtcaccgc atttctagga gccccgttct tcatcgtggt ccttcgcagt gttcgaggca
     2221 gccagtgatc gcctggcacg aggtagcggt cacctttggt gggcctcctg tggtgggccc
     2281 tctctcgctt acagtcgtcg acggggagtg gcttgctttg atcgggccca acggcgccgg
     2341 caagtcgaca ttgctcaagg ccgcggttgg aattgtcgag cacagcggat cggtccaatt
     2401 tggtgggcga gcatcgagag ccggcttgga tgtcgcttgg atgccacagc ggccctacct
     2461 gccggaagag atgtcggtga ccgactacgt gcttctgggt cgaactccgc acctcggtcg
     2521 attcggcacc gagcgagaag ccgacctcga ctcggcgcag cgctcaatac agcggctgga
     2581 actgggggca ctcgcagatc gcgccctggc gacactgtcc ggaggagagg cacaacgcgc
     2641 tgtcctggcc cgggcgatcg cccaggaggc gccggtgctg cttctcgatg agccgacttc
     2701 caacctcgac atcggccact cgctggacgt tctcgagatc gttgacgacc tgaggttgag
     2761 cgatgggctc accgtcgtca ccgcactcca tgatctcacc cttgccggaa ggttcgccgg
     2821 gcgggtcgtg atgcttgcgt cggggaaggt cgttgccgac ggatccccgg atgaggtgct
     2881 gaccgaggag ctgcttcgat ctcactacgg tgccggtatt cgagtggttg acaacgggga
     2941 aggcccgact gtgataccca ccaggaaccg ccgtgtctga tccgacgaag cc
//
LOCUS       DBNHXU010000228         7425 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2650480, whole genome shotgun sequence.
ACCESSION   DBNHXU010000228 DBNHXU010000000
VERSION     DBNHXU010000228.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7425)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7425)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7425
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2650480"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2843)
                     /locus_tag="AC161X_05670"
     CDS             complement(<1..2843)
                     /locus_tag="AC161X_05670"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4989074.1"
                     /translation="MQTFLFTDIEASTRHWEEQPVAMASALARHDSIMTGAIDRAQGT
                     LLKTTGDGVIARFDSAGSALAATLDAQRALSGEAWGVTGPLRVRMGIHAGETESRNGD
                     FFGPSMNRAARIMAAGHGGQVLVSGAAAELAGEHLPHGAVLRDLGMHRLKDLTLPEHL
                     YQLVHPDIESEFPAPVTLDSRPHNLPLQATEFLGRRNELAAIQVMLESPNTRLLTIAG
                     PGGAGKTRLGLQVAAEQLDHFRDGVFFVDLSAELDPGSVFEAIVRTLELPVLGGGDAL
                     QILKSRLRDLEMMLVLDNFEQVTEAAVGLSELLQHAPHLRVVVTSRETLRVRAEHVFP
                     VPPLSLPHPRDPVASIAESEAVQLFTERAVAVRPGFTLTDENAAVVAEICLRLDGLPL
                     AIELAAARLKVFTPTDLLDRLRKRLDVLGAGGRDLPDRQRTLWGAIGWSYELLNEDER
                     RLFELMSVFSTTDLPGLETVASDMDAGLVLDSLASLVDKSLVRSDDDVESQRFAMLLM
                     IKEFAAERLADTPDREAAVRKAHADYFSRLTVDIEERLGGPERDAALEDVESEIGNLR
                     TAWRFWVDAGDVDRMLELIGSLWSLYESKGWYHSAIELASDALEVMAQAGSPPELAPD
                     ELVMRTMLARTLMAVRGYDVEVEAEFKRALEISKTVHGSAAQRFPVLRALATYYMGIA
                     DFAQGMEYGREILELGERESDLAIQMEGHYVYGVGMAFTGDLEHGLPHLDRAIELHDP
                     RARSASRFRLGPNTGVVARVASGLVLWQCGDLEKSVSRVSAALDIAREIDHPYSIAYA
                     LYHNAFLALQRSRFEECIAIAKDLAEVSEEHDYVVWATLAIFLEGICRTVLGETERGL
                     EMTERAIDLYQGLTTPPVFWPLILAFRALVHALAGQPERALGLIEEAITIGGPDDMTS
                     PEFRIVRGDVLRMLPTPDHASAEDSYQSAIRGA"
     gene            complement(2914..4104)
                     /gene="dinB"
                     /locus_tag="AC161X_05675"
     CDS             complement(2914..4104)
                     /gene="dinB"
                     /locus_tag="AC161X_05675"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013286460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HIR4989075.1"
                     /translation="MRSEASILHADLDAFYASVEQRDNPGLRSRPVIVGGGVVLAASY
                     EARAYGVRTAMGGGLARRLCPDAIVVEPRISAYSEASKAVFEVFKHTTPLVEGISIDE
                     AFLDVGGLKRISGSPIEIARRLRQEVLTEVGLPITVGIARTKFLAKVASAVAKPNGLL
                     VVAPGSEFDFLHPLPVERVWGVGKVTADKLHERGINTVGDIAELAEPSLVAMLGRAAG
                     RHLHALAHNRDPRPLEVGRRRRSIGSQRALGLGPRSIEDLDTILVGLVERVSRRLRTA
                     GRVGRTVVLRLRFNDFSRATRSHSFSRPTARTQTILTALRSLLAGVVPLIDSQGLTLI
                     GIAIANLDDDDALQLPLPFDREPGLALDEAMDEVNDRFGSATVTRAVLLGNDPGLTVP
                     LLPD"
     gene            4174..4362
                     /locus_tag="AC161X_05680"
     CDS             4174..4362
                     /locus_tag="AC161X_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013283729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR4989076.1"
                     /translation="MTDVPRLVRPKEGRVIAGVAAGLADRFGMSRNTMRLLFVLSLIL
                     PGPQILIYLILWIVIPEE"
     gene            complement(4366..4596)
                     /locus_tag="AC161X_05685"
     CDS             complement(4366..4596)
                     /locus_tag="AC161X_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005483428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4287 domain-containing protein"
                     /protein_id="HIR4989077.1"
                     /translation="MPSQDSKAKVTGPASYFPSIEKKYGRPISEWQEMIRSSPLTKHM
                     ELVNWLKSDYGMGHGHANALVGYTLDEKRWKA"
     gene            4698..5603
                     /locus_tag="AC161X_05690"
     CDS             4698..5603
                     /locus_tag="AC161X_05690"
                     /inference="COORDINATES: protein motif:HMM:NF045432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR4989078.1"
                     /translation="MEVTHPERVVFPDDGITKGEVVAYYTKVASRMLPFIEGRALSVE
                     RFPQGIGGKGFMQKNAPDHYPEDLIGRHELPKEGGGTTVYPVVNTSEGIEFFANFGAI
                     TFHVPSIRIVDEDHPDWVIWDLDPPPERVALVREAAVAMRSFLEGYGIPTVLLASGSK
                     GYHLRARLRPGLDLEAVARIARGTAELAVASHEELLTVAFRKADRGERVFVDWMRNTG
                     RSTSVAPWSLRPRSGAPVAAPLAWEELTSVEPDGIQLRGVDARMDVDPWSALKPIDLT
                     SVLEHVDRDLTDAGIQLEPFDRFRS"
     gene            5636..6676
                     /locus_tag="AC161X_05695"
     CDS             5636..6676
                     /locus_tag="AC161X_05695"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4989079.1"
                     /translation="MNRNVFEPIRTKRLLIRPFQSGDAVALEARRNDPLVAKYQNWAV
                     PFTAEQAKTVESELVAMDGPQDEEWWMAVVADAESGETLGDLALHLSQKARTAEIGYT
                     FASEHWGHGYAVEAMEALVAYLFDVLGVTRVFGLLNPENIASAMVMERTGFLYEGHTK
                     LSFWLEDANEDDWIYGLIHEDWDKWRNRPQAPPKDVSLVELVTENERVVSRLGTHKTQ
                     EAFVAPMVWSFADALFPEVIDGAPVVPWMRAVVADDEVVGFVMLALRTEHHPEPYLWR
                     LLIDRLHQRRGIGGRVLDLIAKECLEMGDTTLVTSWGEGKGSPRPFYLSHGFVPTGKV
                     IDDETEGRKQLS"
     gene            6705..7421
                     /locus_tag="AC161X_05700"
     CDS             6705..7421
                     /locus_tag="AC161X_05700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989080.1"
                     /translation="MVFRFDGVNHAGQEWRAAGAIVTFGKQIEAIRPGRFRTDGTVAS
                     KQHDLNSPTSDHRPSPRVGLGTVRALDFAEGTRGFVDEVAETLRLSRDPRILYVIHDR
                     RKFSSTRTKKFPAFEWRPYTGENGHLTHGHISVVPGEVGEQTQEFAVAPGQAIGQIQD
                     VEIGKEEMAFTAEEEAFLKELFRVVVEDMGSNAKFAKSAILDIRKDIITRGELQAAFK
                     DLPKGDVDAAIAEVIKRLNA"
BASE COUNT         1390 a         2264 c         2379 g         1392 t
ORIGIN      
        1 tgggcgcctc ggatcgccga ttgataggag tcctcggcgc tggcgtggtc aggcgtcggc
       61 agcatcctca atacgtcgcc tctgacgatc cgaaactcgg gtgaggtcat gtcatcgggg
      121 ccaccgatcg ttatcgcctc ttcgatcaat cccaacgctc gctcgggttg tccggcgagc
      181 gcgtgcacca gggctctgaa ggccaggatc agcggccaga acactggagg tgtggtcagc
      241 ccctgataga ggtcgatcgc tctctcggtc atctccaacc cgcgttcggt ctcaccgagg
      301 accgttcgac agatcccctc gaggaagatc gccagagtcg cccaaaccac gtagtcatgc
      361 tcttccgaaa cctcggccag gtccttcgca atcgcgatgc actcctcgaa cctgctcctc
      421 tgcagtgcga ggaaggcatt gtgatagagg gcgtaggcga tgctgtaggg gtggtcgatt
      481 tctctggcga tgtcgagggc tgcgctgacc cgggaaaccg atttctccag gtctccgcac
      541 tgccagagga ccagtccgga ggcgacccgc gccaccaccc cggtgttggg gccgagacga
      601 aagcgtgacg cgctccgagc acgggggtcg tggagctcga tggcgcgatc gaggtgaggt
      661 agaccgtgct cgaggtcgcc cgtgaacgcc atgcccacgc cgtagacata gtggccttcc
      721 atttgtatgg ctaggtcgct ctcccgttca ccgagctcga ggatctctcg cccgtactcc
      781 attccctggg cgaagtcagc gatccccatg tagtaggtcg ccaaagccct gagcaccggg
      841 aaacgctgtg cggcgctgcc gtgcacggtt tttgagatct cgagcgcccg cttgaactcg
      901 gcttcgactt ccacgtcata gccacgtacc gccatcaggg ttcgggccag catcgttcgc
      961 atgaccagct cgtcgggagc caattccggt ggcgagccgg cttgggccat cacctcgagg
     1021 gcgtcggacg ccagctcgat cgcgctgtgg taccagccct tcgactcata aagagaccac
     1081 agactcccaa ttagttcgag catccggtcg acgtcaccgg cgtccaccca gaatcgccat
     1141 gcggtcctta agttgccaat ctccgactcc acatcttcga gggctgcatc ccgctcggga
     1201 cctccaagcc gctcttcgat gtcgacggtg agcctggaga agtagtcggc gtgcgctttg
     1261 cgcacggctg cttcccgatc gggagtgtcg gcgagccgct cggccgcgaa ctccttgatc
     1321 atgaggagca tggcgaaccg ctgtgattcg acgtcgtcgt cgctccgaac gaggctcttg
     1381 tcaacgagcg aggccagcga atcgaggacc agccctgcat ccatgtcgga tgccaccgtc
     1441 tccagaccgg ggagatcagt ggtggagaac accgacatca gctcgaacag tcgccgctcg
     1501 tcttcgttca acagctcgta gctccacccg atcgcccccc agagggttcg ctgccggtcg
     1561 ggcagatctc ggcctccggc acccaggaca tcgagccgct tgcgaaggcg gtcgaggagg
     1621 tcggtcggtg tgaacacctt gagtcgcgcg gctgccagtt cgatggccaa tgggaggccg
     1681 tcgagtcgca ggcatatctc ggcgacaact gccgcattct cgtcggtgag ggtgaagcct
     1741 gggcgcaccg caacagctcg ttcggtgaat agctgcaccg cttcggactc ggctatcgag
     1801 gcgacgggat cgcgtgggtg aggaagggag agcggtggaa ccgggaacac gtgctcggcg
     1861 cgtacccgga gggtttcccg actcgtcacc accactctca ggtgcggtgc atgttggagg
     1921 agctcggaga gacccacggc cgcttcggtg acctgctcga agttgtcgag caccagcatc
     1981 atttcgaggt cacggagcct cgacttgagg atctggagcg cgtcaccgcc accgaggacc
     2041 ggcagctcga gggtccggac gatcgcctcg aagaccgacc ccgggtcgag ttcggccgag
     2101 agatcaacga agaacacgcc atcgcggaag tgatccagct gctcggccgc cacctggaga
     2161 ccaagacgag tcttacctgc cccacccggg ccagcgatcg tcagcagcct ggtgtttgga
     2221 gactccaaca tgacctggat ggcggccaac tcattgcgcc gtcccaagaa ctccgtggcc
     2281 tgtagcggaa ggttgtgggg ccgtgaatcg agggtgaccg gtgccgggaa ctcgctctcg
     2341 atgtcggggt gcaccagttg gtagagatgc tcgggaagag tcaggtcctt gaggcggtgc
     2401 atgcccaggt ctcgcaatac cgcgccgtgt gggagatgct ctccagcaag ctccgccgcg
     2461 gcccctgaaa ccaacacctg accgccgtga ccggcggcca tgatccgtgc cgcccgattc
     2521 atcgaaggcc cgaaaaagtc accgtttctg ctctcggtct ctccggcgtg gatgcccatg
     2581 cgaacccgca gcggtccggt tacgccccag gcctcaccac tcagagccct ttgggcatcg
     2641 agggtggccg caagcgcgct gccggcggag tcgaagcggg caatcacccc gtcgccggtc
     2701 gtcttgagga gtgtgccttg ggcccgatcg atggctccag tcatgatcga atcgtgtcgc
     2761 gcgagggcgg atgccatggc gacgggctgc tcttcccagt gacgcgtgct cgcctcgatg
     2821 tcggtgaaca gaaacgtctg catcagcgag gaatataggc ggacacacga ttcctccctc
     2881 cgggcgacag aatgctgctg gaacctgatc cgactagtcg gggagcagag gaacggtcag
     2941 gcccggatcg ttgccgagga ggaccgcccg ggtcacggtg gccgatccaa accgatcgtt
     3001 gacctcatcc attgcctcgt caagagccaa tcctggctcc cgatcgaatg gcaacggcaa
     3061 ttgcagcgca tcgtcatcgt cgaggttcgc gatggcgata ccgatcaggg tgaggccttg
     3121 actgtcgatg agcggaacca caccggcgag cagtgaacgg agtgcagtga ggatggtctg
     3181 ggtgcgggcc gtcgggcgcg agaagctgtg cgaacgggtg gcccgggaga agtcgttgaa
     3241 tcgaagtcgc aggacgacag ttcgccccac ccttcccgca gtacggagtc ggcgactgac
     3301 acgctcgacc agcccgacca ggatcgtgtc gaggtcctcg attgatcgag gtcccagtcc
     3361 gagagctcgt tgcgatccga tcgaacggcg gcggcggccc acctcgaggg gacgtgggtc
     3421 gcgattgtgg gcgagggcgt ggaggtgccg gcccgctgcc cgacccagca tcgccaccaa
     3481 cgacggctcg gccagctcgg cgatgtcccc gactgtgttg attccacgct catgcagctt
     3541 gtctgcggtg actttgccca ctccccaaac cctttccacc gggagagggt gcaggaagtc
     3601 gaactcggat ccgggtgcca ccaccaggag cccatttggc ttggctaccg cgctggccac
     3661 cttggccagg aatttggtcc gggctatccc gaccgtgata ggcaggccta cttcggtgag
     3721 cacctcttgt ctcagacgcc tcgcgatttc aatcggtgag cccgagattc tcttcaaccc
     3781 gcccacgtcc aagaaggcct cgtcgatgga gatcccctct accaggggag tggtgtgttt
     3841 gaagacctcg aacacggctt tgcttgcctc ggaatatgcc gagatccgtg gctcgacaac
     3901 gattgcgtcc gggcagaggc gacgtgccag tccaccaccc atcgcggtcc tgactccata
     3961 ggctctggct tcgtagctgg ccgccaggac aacaccaccg ccgacgatca cggggcgtga
     4021 gcgaagcccc gggttgtccc gttgctccac cgacgcatag aacgcgtcga ggtcggcatg
     4081 gagaatggac gcctcggatc gcatcgaaca tatgttcgct caaagcgcga gtgaaggcaa
     4141 gagggctcgt gggaagcggg taggttgccg gttatgaccg atgtaccgcg tctcgtgcgt
     4201 cccaaagagg gccgggtgat tgctggcgtc gcggctgggt tggcggatcg attcgggatg
     4261 agccgcaaca cgatgaggct cctgttcgtc ctctcgctga ttctgcccgg ccctcagatc
     4321 ctgatctacc tcatcctctg gatcgtcatc cccgaggagt gaggctcagg ctttccacct
     4381 cttctcgtcg agggtgtagc cgacgagagc gttggcatga ccatggccca tcccgtagtc
     4441 agacttcagc cagttgacca gctccatgtg cttggtgagt gggctggacc ggatcatctc
     4501 ctgccactcg gagatcggtc ggccgtactt cttctcgatc gacggaaaat aagaagcggg
     4561 gccggtgact ttggctttgg aatcctgact tggcatcggc caatggtatc cgcacctggg
     4621 gctccccact gccggcttct cgggctctct cctccaacgc tcactctgtt cgctgggggg
     4681 agaaataggc tggtctcatg gaggtgaccc accctgagcg cgttgtcttt cccgacgacg
     4741 ggatcaccaa gggcgaagtg gtcgcctact acacaaaggt tgcgagccgg atgctgccat
     4801 tcatcgaggg cagagccctt tcagtggagc gctttccgca ggggatcggg ggcaaagggt
     4861 tcatgcagaa gaacgcaccc gaccactacc cggaagacct catcgggcgt cacgagctcc
     4921 caaaggaggg aggtggcacc acggtctacc cggtggtcaa cacgtccgag ggcattgaat
     4981 tctttgccaa cttcggggcg atcacattcc atgtcccgtc gattcgcatc gtggacgaag
     5041 accatcctga ctgggtcata tgggatctcg atcctccgcc ggaacgggtg gcgctggttc
     5101 gggaggccgc ggtcgcgatg cgctcattcc tcgagggcta cgggattccg acggtgctcc
     5161 tcgcttcagg atccaagggc tatcaccttc gagcccgatt gcggccaggc ctcgacctcg
     5221 aagcggtggc ccggatcgcc cgaggcactg ccgaactggc ggtggcttcc catgaggagt
     5281 tgttgaccgt tgcgttccgc aaggcggacc gtggggagcg ggttttcgtt gattggatgc
     5341 gcaacacggg tcggtcgact tcggtggcac cctggtcgct tcgaccccgc tcgggcgccc
     5401 cagtggcggc cccactggcc tgggaggagc tgacatcggt cgaaccggat gggattcaac
     5461 ttcgtggcgt tgacgcgcgc atggacgtcg atccatggtc cgcgttgaag ccgatcgacc
     5521 tgacaagcgt tctggagcac gtggatcgag acctgacgga cgccggcatc caactcgagc
     5581 cgttcgaccg tttccgctcc tgaggaaatc ggtcgggaga tcgtccttgg cctggttgaa
     5641 tcgcaacgtg ttcgagccga tccgcaccaa acgtttgctg atccgaccct tccagtcggg
     5701 tgacgcggtc gcgctcgaag ccaggcgtaa cgacccactc gtcgccaaat atcagaactg
     5761 ggctgtgccg ttcactgcgg aacaggccaa gacggtcgaa tccgagttgg tcgcaatgga
     5821 tgggcctcag gatgaagagt ggtggatggc cgtcgtcgct gatgcggaga gcggggagac
     5881 cctcggtgat ctggcactgc acctgagcca gaaggcgcga actgcagaga tcgggtacac
     5941 cttcgccagc gagcactggg gccacggcta cgccgtcgag gccatggagg ctctcgtcgc
     6001 ctacctattc gatgtgctcg gtgtcaccag ggtctttggc ttgctcaatc ctgaaaacat
     6061 cgcctcggcg atggtgatgg aacggaccgg attcctctac gagggccata ccaagttgtc
     6121 cttctggctg gaggatgcga atgaggacga ctggatctac ggactgatcc atgaggattg
     6181 ggacaagtgg cgtaaccgtc cgcaggctcc accaaaggac gtgagccttg tcgagctcgt
     6241 caccgagaac gagcgcgtcg tgtcgagact gggcacccac aagactcagg aggcgttcgt
     6301 agctccgatg gtctggtcgt tcgccgacgc cctgttcccg gaggtgatcg acggcgcacc
     6361 ggtggtgccc tggatgcgag cagtagttgc ggacgacgag gtcgtcggct tcgtgatgct
     6421 tgctctccgc accgagcatc accccgagcc atacctctgg cggttgctga tagatcgtct
     6481 ccaccagcgg cgggggatcg gaggccgcgt cctcgacttg attgcgaagg agtgtctgga
     6541 gatgggcgac accaccctcg tcaccagctg gggcgagggc aagggatcac cccgaccctt
     6601 ctatctcagt catggcttcg ttccgacagg caaggtgatc gacgacgaga ccgagggtcg
     6661 gaagcagctg agctagctgc cccgatgcgg ttaggtttcg ccacatggtt ttcaggttcg
     6721 acggggtgaa ccatgccggt caggaatggc gggcggcggg ggcgatcgtc acctttggca
     6781 agcagatcga agcgatacgt cccggcaggt ttcgcaccga cggtacggtc gccagcaagc
     6841 aacacgacct gaacagcccg acgtcggacc accgtcccag cccgagagtc ggcctgggga
     6901 cggtgcgcgc actcgacttc gcagaaggaa ctcgcggctt cgtcgacgag gtagctgaga
     6961 cactcaggtt gtcccgggat ccgcgcatcc tctatgtcat tcacgaccgc cggaagttct
     7021 cttcgacccg caccaagaaa ttcccggcct tcgaatggag gccctacacc ggggagaacg
     7081 ggcatttgac gcacgggcat atatcggtag ttccgggcga agtgggcgag cagacccagg
     7141 agtttgccgt ggctccgggc caagcgatag gtcagatcca ggatgttgag atcggaaagg
     7201 aagagatggc gttcactgcc gaagaggaag cattcctcaa agagctgttc cgggtagtag
     7261 tcgaggacat ggggtccaat gccaagttcg ccaagtcggc gattctggac atacggaagg
     7321 acatcatcac ccgaggcgag ttacaggctg ccttcaagga tctgcccaaa ggtgatgtcg
     7381 acgctgcgat cgcagaagta atcaaaagac tgaacgcctg acgtc
//
LOCUS       DBNHXU010000229         3051 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2650522, whole genome shotgun sequence.
ACCESSION   DBNHXU010000229 DBNHXU010000000
VERSION     DBNHXU010000229.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3051)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3051
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2650522"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1076)
                     /locus_tag="AC161X_05705"
     CDS             complement(<1..1076)
                     /locus_tag="AC161X_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIID/LytB domain-containing protein"
                     /protein_id="HIR4989081.1"
                     /translation="MADIDDWEFEGGGWGHGVGLSQYGTLAQAQDGRNATQILQFYYT
                     GTSIASVPGTHWTTQPNKLLVGLVSNATTVILGAVGGPLTICQPATTCPPVSPPNEPR
                     GPAGFADVTIDPGETWRFERFADDPNKCRFRKTDPVAAAGNLGWGPCNAKLTKAAGTT
                     VRYVVNGKEYARGELRFTDSTAGFHAVAALDLEQYLYGLAEVPSSWPSEALKAQAIIG
                     RSYAVATAVDRGGASGSTTLSSCGCHLKSTTADQVYAGWSKEVGGAAWVSAVDATAGD
                     IITHPQSTYALDIAKAFYSSSNGGASEDNEDVWGAPALPWLRSVEDPWSVDPVNPLAH
                     WTILVNTANVATYLGWDKMYTGQL"
     gene            complement(1189..2886)
                     /locus_tag="AC161X_05710"
     CDS             complement(1189..2886)
                     /locus_tag="AC161X_05710"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR4989082.1"
                     /translation="MLSLATFALMVALLPTAALAPGGTFIDDDGSVHEGGIEAVALEG
                     ITVGCNPPFNDRFCPDRLLTRAEMATMIARALSLPASSTNHFVDDNGHILEGAINRLA
                     DAGITQGCNPPTNNRFCPDRNLTRAEAAAFISRAFGLPVSGHDFFIDDNGHVLEGSIN
                     RIAEAGITVGCNPPTNNRFCPNRVLTRGEMATLLTRAMSLTATKPSARPPLDWDLVVG
                     GLDTPIQVLNPPGEDRLLIAEQSGLVRIFQSGALQSQPFLDIRSSVFFSVERGFLSMA
                     LHPDYPTDRRMFAWYSRPLRSGGSGDHTNYLVEFDIAADLQTASSPRVLLAVDHPANN
                     HNGGFVSFGPDGYLYLSIGDGGGGNDPSGRSRNLNTLFGKMLRIDVDGAAPYAIPSDN
                     PYVGAPGRDEVWASGLRNPWRWSMDNGNLYIGDVGQGAREEIDVVRVNPVGYDFGWSR
                     YEGTLCNPNDSDPSCSKSGLTMPVAEYGRSEGSTVTGGVVYRGPTVRSLSQYYIYADY
                     GSGLVRGFRLLNGKPVESRDLSPRLRMTGFVSFAPDADGELLAVSIHDGAVYRLVGG"
BASE COUNT          589 a          937 c          980 g          545 t
ORIGIN      
        1 atgagctgac cggtgtacat cttgtcccag ccgaggtagg tggccacgtt tgcggtgttg
       61 accaggatcg tccaatgcgc cagcgggttc accgggtcca ccgaccaggg atcctccacc
      121 gagcgcaacc agggcagagc cggcgcaccc caaacatcct cgttgtcctc gctggccccg
      181 ccgttggacg aggagtagaa agccttggcg atgtccaggg cgtaggtgga ctggggatgg
      241 gtgatgatgt cgccggcggt ggcgtcgacc gctgacaccc aggcggcccc tccgacttcc
      301 ttgctccagc cggcgtagac ctgatcggcg gtggtcgact tgagatgaca cccgcaactc
      361 gaaagggtcg tgctcccact ggcaccccca cgatccacgg cggtggcgac cgcatagctc
      421 cgcccgatga tcgcctgggc cttcagcgct tcggaaggcc atgacgaggg gacctcggcc
      481 agaccgtaaa ggtattgctc gagatcgagg gcagccacgg catggaaacc ggcggtggag
      541 tcggtgaatc ggagctcgcc ccgggcgtac tccttgccgt tgaccacata gcgaaccgtg
      601 gtcccagcgg ccttggtcag cttggcgttg cagggccccc aaccaagatt cccggcggcc
      661 gcgactggat cggtcttgcg gaagcgacac ttgttcggat cgtccgcgaa ccgctcgaac
      721 ctccaggtct caccggggtc gatggtgacg tcagcgaaac cagccggtcc gcgaggctca
      781 ttgggtggac tcaccggagg acaggtagtc gccggttggc agatggtgag aggtcccccg
      841 accgcgccca aaatgaccgt ggtcgcgttg gaaaccagac cgaccaggag cttgttgggc
      901 tgcgttgtcc aatgggtgcc aggcaccgac gcgatcgacg tccccgtgta atagaactgg
      961 agtatctgag tggcgttgcg cccatcctgg gcctgtgcca acgtcccgta ctgggagaga
     1021 ccgacgccat ggccccagcc gcctccctca aattcccagt cgtcgatatc cgccatcgcg
     1081 gggaaagcaa ccgaaagcag gcctatggcg gtcgcgaaaa cgacgattga gcgttgcaac
     1141 ctcacaatcg gcaccatcgg ccttcgggca tgacgcctga aggaaatatc aaccacccac
     1201 caggcggtag acggcgccgt cgtgaatgga gacggcgagc aactcgccgt cagcgtcagg
     1261 ggcgaatgag acgaaacccg tcattcggag tctcgggctc aggtcacgtg attcaaccgg
     1321 tttgccgttg aggagccgga acccgcgcac caacccggag ccgtagtcgg cgtatatgta
     1381 gtactggctg agcgatcgca ccgtgggccc ccgatagacg acgccgccgg tcaccgtcga
     1441 cccttccgat ctcccgtact cggcgaccgg catggtcaga ccggacttgg agcaactcgg
     1501 gtcgctgtca ttcgggttgc agagggtccc ttcatatcgg gaccagccga agtcataacc
     1561 aaccgggttc acacgcacga catcgatttc ttcgcgagcg ccttggccga cgtcaccgat
     1621 atagaggttg ccgttgtcca tcgaccagcg ccacggattg cgcaggccgg aagcccagac
     1681 ctcatcccgc ccgggtgctc caacgtatgg gttgtcagag gggatcgcgt acggggctgc
     1741 tccgtcgacg tcgatgcgga gcatcttgcc gaaaagcgtg ttcaggttgc gggaccgccc
     1801 cgagggatcg ttgccgccgc ctccgtcgcc gatactcaga tagaggtagc catcgggccc
     1861 aaaggagaca aaccctccgt tgtggttgtt cgccggatga tcgacggcga gcaatacacg
     1921 gggcgaggag gcagtctgga gatcggcggc aatgtcgaat tcgaccagat aattggtgtg
     1981 gtcgccgctc ccgcccgatc ggagcggtct cgagtaccag gcgaacatcc gacgatcggt
     2041 tgggtagtcg ggatggagcg ccatggacag gaaaccgcgc tcgacactga agaacaccga
     2101 gctgcggatg tcgaggaaag gctgggattg caaggcccca ctctggaaga tgcggaccag
     2161 cccgctctgc tcggcaatga gcaaccgatc ctctcccggc gggttgagga cctggatcgg
     2221 ggtatcgagt ccgccgacta ccaggtccca atccaacggt gggcgagccg acggcttcgt
     2281 ggcggtcaag ctcatggctc tggtcaggag cgttgccatt tcgcccctgg tgagaacccg
     2341 gttcggacag aacctgttgt tggtcggtgg gttgcatcca acagtgatcc cggcttcggc
     2401 gatccggttg atcgagcctt cgagcacatg tccgttgtca tctatgaaga agtcatgccc
     2461 cgaaaccggc aatccaaacg ccctcgagat gaaggcggcc gcctcggccc gggtcaggtt
     2521 tcggtccggg cagaaccggt tgttggttgg agggttgcaa ccctgggtga tcccggcgtc
     2581 ggccagccgg ttgatcgctc cctcaagaat gtgcccgttg tcgtcgacga agtgattagt
     2641 gctcgaagcc ggcagagaca acgccctcgc aatcatcgtc gccatctcag ctctggtcag
     2701 cagacgatca gggcagaacc ggtcgttgaa tggtggattg catccgaccg tgattccctc
     2761 gagggcgact gcctcgatac caccttcgtg gacgcttccg tcgtcgtcga tgaaggttcc
     2821 tcccggggca agtgccgccg tcgggagcaa cgcgaccatc agtgcgaagg tagcgaggga
     2881 aagcatcgac ctcgaccaag gtaaccgctt cgcccggcaa agccagaaag tctgggagga
     2941 ttagacccga tgccgttcct cctgtctctg ttgctggcaa ttggcacctt gggacctcaa
     3001 cccgccgccc cggatgagag cgtgacggtc aggctgctga ttccggagga g
//
LOCUS       DBNHXU010000230         2429 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5430911, whole genome shotgun sequence.
ACCESSION   DBNHXU010000230 DBNHXU010000000
VERSION     DBNHXU010000230.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2429)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2429
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5430911"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..296
                     /locus_tag="AC161X_05715"
     CDS             <1..296
                     /locus_tag="AC161X_05715"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR4989083.1"
                     /translation="ATSTEAAAALTRLGWMAFDGNGEVDTALNLLDQALTIEPDAPVA
                     LYLKGRVQWCGAGDTEAAIALFDEVLANPSLPVESRTEIEADLEQATAGEACA"
     gene            293..886
                     /locus_tag="AC161X_05720"
     CDS             293..886
                     /locus_tag="AC161X_05720"
                     /inference="COORDINATES: protein motif:HMM:NF020123.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HIR4989084.1"
                     /translation="MNGRLLRWVGIGVIAVTVVLAVVLASRFGTDPGLVDSPLIGQPA
                     PDFDLETLDGTGRLALADLSGDIVVVNFFASWCLECRNEHSDLVATSDSFAGDDVSFV
                     QIAYQEQPEQSLQYLAEAGTSEATSYLADPGSRTAIAYGVYGIPETFFIDPEGTVVGK
                     IIGQADALTLGATIDAIKRGEKPGQQVTGDTQQRPDS"
     gene            complement(901..2298)
                     /locus_tag="AC161X_05725"
     CDS             complement(901..2298)
                     /locus_tag="AC161X_05725"
                     /inference="COORDINATES: protein motif:HMM:NF015368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003847 -
                     1-alkyl-2-acetylglycerophosphocholine esterase activity
                     [Evidence IEA];
                     GO_process: GO:0016042 - lipid catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR4989085.1"
                     /translation="MRRFELLMVFGALFAVGWPAVFGVRTRRGIVALVLLVLFVVHWR
                     VEGLRWQMLPLYGVCLGLAIGDIIVVERRLDWTSRIARGIFGVAGVGLAAILPLVLPV
                     PELPLPSGPEAIGTLTFDLVDAEREETYGPAPGGPRKLRVQVWYPAESSDEVDPTAWA
                     EDWDVVAPALSELLGYPGWFLNYTRYTPSHATVSLPVAEGTFPIVIYSHGWNGFRAIT
                     VNQIENLVSHGYVVIAPDHTYSAVTTRFENGDVVPYDPEALPEEADVSEEEYAEASGN
                     LMNVYVADLTSILNALDLGEDGPFGALATSADLSKIGIYGNSIGGGAAVEVCLLDERC
                     DAVLGLDPWVEPIPDRTISASATRPALFMRSDEWQANENDAVLRGIAERSENVTYWIG
                     VDGTVQNDFTVAPLWSPISEQLGLTGTIPAGRVIPIVDRYVLGFFDVFLLETGSAALD
                     TASFEEVTVEVIRPD"
BASE COUNT          477 a          820 c          737 g          395 t
ORIGIN      
        1 cggcgaccag caccgaggct gccgccgccc tcacccgtct cggatggatg gccttcgatg
       61 gcaacggcga ggtagacacc gctctcaacc tcctcgacca ggccctcacc atcgagcctg
      121 acgcgcccgt cgccctctac ctgaaagggc gggtccagtg gtgcggagcc ggggacaccg
      181 aggcggccat cgccctcttc gacgaggtcc tcgccaaccc ctctctgccc gtcgagtcac
      241 gaaccgaaat cgaggcagac ctcgagcagg cgaccgccgg cgaggcttgc gcatgaacgg
      301 gcgcctgctg aggtgggtcg gaatcggtgt gatcgcggtc accgttgtcc tcgcagtcgt
      361 ccttgcctcc cggttcggaa ccgacccggg gctggtcgac tccccattga tcgggcagcc
      421 tgcacccgac ttcgacctgg agaccctcga cgggacaggc cgacttgcac ttgccgacct
      481 gagcggtgac atcgtcgtgg tcaacttctt tgcatcctgg tgtctcgagt gccgcaatga
      541 gcacagcgac ctggtggcaa cctcggactc gttcgccggg gatgacgtga gcttcgtcca
      601 gatcgcctat caagagcagc ccgaacagtc cttgcagtat ctcgccgagg cagggacttc
      661 ggaagccacc agctacctgg ccgatcccgg aagccggact gccatcgcct acggcgtata
      721 tgggatcccc gagaccttct tcatcgaccc cgaggggacg gtcgtcggaa agatcatcgg
      781 ccaggccgac gcgctgaccc tgggtgcgac catcgacgcc atcaagagag gcgagaagcc
      841 cggtcagcag gtcaccggcg acacccagca gcgccccgac agctaagccc gcgatcgcta
      901 tcagtcgggg cggatcacct cgaccgtgac ctcttcgaag ctggcggtgt cgagggccgc
      961 cgaccccgtc tccaacaaga agacatcaaa gaacccgagg acgtagcggt cgacgatcgg
     1021 gatcaccctt cccgccggga tggtgccggt tagccccaac tgctcgctga tgggagacca
     1081 gagaggagcg acggtgaagt cgttctggac ggtgccatcg acaccaatcc aataggtcac
     1141 attctcactg cgctcagcga tcccccggag cacggcgtcg ttctcgttgg cctgccactc
     1201 atccgaccgc atgaacaggg cgggacgggt ggcggatgcc gaaatcgtgc gatccgggat
     1261 cggctcgacc cagggatcga ggccgagcac ggcgtcgcaa cgctcgtcga ggagacaaac
     1321 ctcaactgcc gccccacccc cgatcgagtt gccgtaaatc ccgatcttgc tcaagtctgc
     1381 gctggtggcg agggccccga acgggccgtc ttctcccaga tcgagggcgt tcaggatcga
     1441 ggtcaggtct gccacgtaga cgttcatcag gttgccgctg gcctcggcat attcctcctc
     1501 gctgacatct gcctcctcag gcagggcctc cgggtcgtac ggcaccacat cgccattctc
     1561 gaaacgggta gtcacagccg agtatgtgtg gtcgggggcg atgacgacgt agccatggct
     1621 gaccaggttc tcgatctgat tcacggtgat cgctcggaaa ccgttccaac cgtggctgta
     1681 gatcacgatc ggaaaggtgc cttcggccac aggcagagac accgttgcgt gtgaaggcgt
     1741 gtatcgcgtg tagttgagga accagcccgg atagccgagc aattccgaga gggccggtgc
     1801 caccacgtcc cagtcctcgg cccacgctgt cgggtcgacc tcatccgagg attcagccgg
     1861 ataccaaacc tggactctca gcttgcgcgg cccaccgggg gccggtccat aggtctcctc
     1921 tcgttccgcg tctactagat cgaaggtcag cgtcccgatg gcctcaggac ctgatggcaa
     1981 gggaagctcg ggcaccggaa gcaccagggg caggatcgcc gccagcccaa cacccgcgac
     2041 accgaagatc ccccgggcaa tccggctcgt ccaatccaat cgacgctcaa cgacgatgat
     2101 gtcgccaatc gcaaggccaa gacagactcc gtagagcggc agcatctgcc agcgaaggcc
     2161 ctcgacgcgc cagtggacga caaacaacac gaggaggacg agggccacga ttccccgtcg
     2221 ggtgcggact ccgaaaactg cgggccagcc gacggcaaac agagcgccga aaaccatgag
     2281 cagttcgaat cgcctcaccg gcgccgcgac ctcagttctc ctgcgattcc gtcagcaggc
     2341 tcagccctgg gagagacgtc ccgggatctt ggtgccttga tggaagatca cttgccagcg
     2401 accctcgtcc ctaacccaaa ccgagctcc
//
LOCUS       DBNHXU010000231         4718 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2850805, whole genome shotgun sequence.
ACCESSION   DBNHXU010000231 DBNHXU010000000
VERSION     DBNHXU010000231.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4718)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4718)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4718
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2850805"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..726
                     /locus_tag="AC161X_05730"
     CDS             <1..726
                     /locus_tag="AC161X_05730"
                     /inference="COORDINATES: protein motif:HMM:NF049746.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HIR4989086.1"
                     /translation="RYPPAFMEITSLADLLDVQDLDLQIDRLLDKRKSLPELDAYRLA
                     HEKQAALEKELVEASDILKTMELDFDKSEGELEMLESKLTEHETRLYAGGMSARETEY
                     MRLEVQSLKGQKSAMEERVLALLDDLDPARDAAQKLTDEIGLLTSEKESLEKVIKAEW
                     KQIDAELARKEERKKDAIVPIPPDLLEMYEKLRLSKEGVAIGRFEHGVCGGCHMALSA
                     AEAAEALDAEIPRCVHCRRILVP"
     gene            730..1269
                     /locus_tag="AC161X_05735"
     CDS             730..1269
                     /locus_tag="AC161X_05735"
                     /inference="COORDINATES: protein motif:HMM:NF016214.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HIR4989087.1"
                     /translation="MLFWHAGATLWLFRWIFRDPKVDVRFLLFGAVLPDLVDLLVGTV
                     FLPERFSTGELYFHTLLLPTLYMTVVLLATRRGRRRRAFMAVGVGWLFHLLLDGMWTE
                     PEILFWPFFGWDVAPGVMPYWASAWERALHDPWRWLLDAVGLAYLTWLWFATGLTDRA
                     KRSALFRTGRIPDLVSEDA"
     gene            1262..1924
                     /locus_tag="AC161X_05740"
     CDS             1262..1924
                     /locus_tag="AC161X_05740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989088.1"
                     /translation="MRRRVALVLLVVAAVACRGTESANTTITRGPGASSAVEAVEELH
                     ALLVDGDFAAASSLAVPDHAALASLAEGANSAEVADALETGDEAIAANFWSGFAQGFG
                     EVLSGEIAVDDAGPATNSGVEFHLVNVTPDSGNARHLVTQEMDGHRVDLFASFGAGLA
                     DRLISPVEILVDTPTGDNAVILAALQEVVPSLLLAASDETLNPEARQNVLKLVELITR
                     VG"
     gene            complement(1931..2914)
                     /locus_tag="AC161X_05745"
     CDS             complement(1931..2914)
                     /locus_tag="AC161X_05745"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03557 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR4989089.1"
                     /translation="MTKFAYFCGHEQWQPEVLVEHARIAEDAGFDMVVVSEHFHPWVD
                     DFSASGFAFSTIGAMAAATQHIEFATGVTTPLFRYHPGVVAQAAATLDRLSGGRFTLG
                     VGTGENINEGPLGYDFPNYAERNARMTESLEIMRRLLDGEKLTFDGEYYRTDRAKLYS
                     PPLGAVPILMAAGGPKSATLAGERAQGVIASVKDPAETIENVVTPMQEAARAAERPDP
                     ILLATRWTILAANEEEAWQALYSWRGLRAPGRLEAVDPADLRAMADAMPREEILGRYT
                     MVHSHEDIFSAYRPLVEDLNADIVTFQMASLDQPNLIRLLGSDVLPRLRQL"
     gene            complement(2934..4613)
                     /locus_tag="AC161X_05750"
     CDS             complement(2934..4613)
                     /locus_tag="AC161X_05750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989090.1"
                     /translation="MKTIRLCLAALTIVSCSAIVGETPTETSVGTTQTSEPTTSTPAN
                     RECVRVTGGTTPIATGSIAGDALSMSGEIFVCADDVVVVGEDDVNEVAAAAQLAAALG
                     GPLLHPHPQLASELGRLKPIRIHLIGDVSVVTPPDATVLHHSISSAVDETKLALGIAD
                     ELRLPSTPDASTIVETIGAINARDRVVLPQTSPSSTTVPPEGAFEPIQILEGLAHATE
                     ASTIWLMDAANPVALLSAAGTGRAVGAGVVAYDPADILGHPEVGAALAGKAAESIRYM
                     GTAPEADPWELAVLVKGQQVPGGGFLIYPEGQPRRYVAFYGHPETTGLGVLGHQGPED
                     TLARMQPFLEEYAADGYQIIPTFEMIASVASAKATDDGDYSFEWPISTFDAWIEVARE
                     NDVYVVLDLQPGREDFLTQAKQYEELLKLPFVGLALDPEWRIKDDQVHLVQTGQVTAA
                     EVNTVVDWLGDLVRDNSLPQKMLLIQQFQSRMIVDRETLKARPELQLVIQMDGQGPTA
                     TKDDTYGFLTEGTEDAPWRWGWKNFFTMDPETPTPEYTIAQVPTPVFVSYQ"
BASE COUNT          800 a         1424 c         1607 g          887 t
ORIGIN      
        1 cgctaccctc cggccttcat ggagatcacg agcctcgccg acttactcga cgtgcaggac
       61 cttgatctcc agatcgaccg actcctcgac aagcgcaagt cattgcctga gctcgacgct
      121 tatcgactgg cccacgagaa gcaggctgcc ctggagaagg agctcgtcga ggcctctgac
      181 atcctgaaga cgatggagct cgacttcgac aagagcgagg gcgagttgga gatgttggag
      241 agcaagctca ctgagcatga gacgaggctc tatgcgggcg ggatgagcgc ccgggagacc
      301 gaatacatgc gtctcgaggt gcagagcctg aaaggtcaga agtcggcgat ggaggaacgg
      361 gtcctcgctc tgctcgacga cctcgatccg gcacgggatg cggcccaaaa actcaccgac
      421 gagatcgggt tgctgacctc cgagaaggaa tcgctggaaa aggtgatcaa ggcagagtgg
      481 aagcagatcg acgccgaact ggctcgcaag gaggagcgaa agaaggatgc gatcgtccca
      541 attccgcccg acctcctcga gatgtacgag aagctgcgcc tctcgaagga aggcgtcgcc
      601 atcggacgat tcgaacacgg tgtctgcggt ggatgtcaca tggcgctctc cgctgccgaa
      661 gccgccgaag ccctcgacgc cgagatcccg cggtgcgtcc actgccgtcg gatcctggtt
      721 ccctgagcaa tgctcttctg gcacgcaggg gcaaccctgt ggctgttcag atggatattt
      781 cgagacccga aggtggatgt ccgatttctc ctgttcgggg cagtcctccc cgatttggtc
      841 gatcttctcg tggggactgt cttccttccc gagcgattct cgaccggcga gctctacttc
      901 cacaccctgc tgctccccac gctgtacatg acggtcgtcc tgctcgcgac acggcggggg
      961 aggaggcggc gcgccttcat ggccgtcggc gtcggctggc tgttccacct gctgctcgac
     1021 gggatgtgga ccgagcccga gatcctgttc tggccgttct tcgggtggga tgtcgccccg
     1081 ggcgtcatgc cctattgggc atcggcctgg gaacgggccc tgcacgaccc atggaggtgg
     1141 ctcctcgacg cggtggggct ggcctatctg acctggcttt ggtttgcgac tggcctcacc
     1201 gatcgcgcca agcggagcgc tctcttccgc accgggcgca tcccggatct ggtctctgag
     1261 gatgcgtagg cgggtcgccc ttgttttgct ggtggtagcg gctgtcgcct gccggggtac
     1321 agagagtgca aacaccacga tcacgcgagg cccgggcgcc tcctctgccg tcgaggcggt
     1381 cgaggagctg cacgcccttc tcgtcgacgg tgacttcgcc gcagcaagct cgctggcggt
     1441 gcccgaccat gccgccttgg cttccctggc cgaaggggcg aactcggctg aagtcgccga
     1501 tgcgctcgaa accggcgacg aggcaatagc cgccaacttc tggagcggat tcgcccaggg
     1561 cttcggggag gtgctgtcgg gtgagatcgc cgtcgacgat gccggacctg ccaccaactc
     1621 gggggtggag ttccacctgg tcaacgtcac tcccgactcg ggcaacgccc ggcacctggt
     1681 cactcaggag atggacggac atcgggtcga cttgttcgcg tcgtttggag ccgggcttgc
     1741 cgatcggctg atatcgccgg ttgagatctt ggtggacacc ccgaccggcg acaacgctgt
     1801 gatcctggcc gccctccagg aggtggtgcc ctcgttgctc ctggcggctt cggacgagac
     1861 cctcaaccca gaggcaaggc agaacgtact gaaattggtc gaactgataa cccgggttgg
     1921 ctgatcccgg tcagagttgc cgcaatcggg ggaggacatc gctgccgagg agcctgatga
     1981 gattgggctg gtcgagcgaa gccatctgaa aggtgacgat gtctgcattc aggtcttcaa
     2041 ccaacgggcg ataggccgag aagatgtcct cgtgggaatg caccatcgtg tagcgaccga
     2101 ggatctcctc gcggggcatg gcgtcggcca tggctctcag gtcggcgggg tcaactgcct
     2161 ccaaccggcc gggagccctt agcccccgcc atgagtagag cgcctgccag gcctcctctt
     2221 cgttggccgc gaggatcgtc catcgggttg cgaggaggat cgggtcggga cgttcggccg
     2281 cccgcgccgc ctcctgcatc ggggtcacca cgttctcgat tgtctccgcc gggtctttga
     2341 ccgaggcgat cacaccctgc gcccgctctc cggccagggt tgccgacttg ggcccgccgg
     2401 ccgccatcag aatcggaacc gctccgagag gcggtgagta gagcttggcc cgatcggtcc
     2461 ggtagtactc gccatcgaag gtcagcttct ccccgtcgag gagtcgacgc atgatctcga
     2521 gtgactcggt catacgcgca ttgcgctcgg cgtagttggg gaagtcgtac ccgaggggac
     2581 cttcgttgat gttctcgccg gtaccgaccc cgagggtgaa gcggcccccg gagagacggt
     2641 cgagggtggc cgcagcctgg gccaccactc cggggtgata tcgaaacaga ggagtggtca
     2701 caccggtcgc gaattcgatg tgctgggtgg ccgcggccat cgccccgatc gtcgagaagg
     2761 cgaagcctga ggccgagaag tcgtcgaccc agggatggaa atgctcggac acaaccacca
     2821 tgtcgaaccc cgcgtcctcg gcgatgcggg cgtgctcaac caagacttcg ggctgccact
     2881 gctcgtggcc gcagaaatag gcgaacttgg tcatcgggga tggatggtaa ggctcattgg
     2941 taggacacaa agaccggggt gggcacctgg gcgatggtgt attcgggggt gggggtttct
     3001 gggtccatcg tgaagaagtt cttccagccc catcgccagg gggcgtcttc ggtgccttcg
     3061 gtgaggaacc cgtaggtgtc gtccttggtc gcggtcggtc cttggccatc catctggatc
     3121 acgagttgca gttccggtcg cgccttcagc gtctcccggt ccacgatcat ccggctctgg
     3181 aactgttgga tgaggagcat cttctgggga agcgagttgt cgcgaaccag gtcgccgagc
     3241 caatcgacca cggtgttcac ctcggcggcg gtgacctggc cggtctggac caagtggact
     3301 tggtcgtcct tgatccgcca ttcggggtcg agggcgagac cgacgaacgg caatttcaga
     3361 agttcttcgt actgcttggc ctgggtgagg aaatcctccc ggcccggttg caggtcgagc
     3421 acgacgtaga cgtcgttttc tcgggcgacc tcaatccacg catcgaaggt ggagatcggc
     3481 cattcgaagc tgtagtcgcc gtcgtcggtg gctttggcgc tggcgacgct ggcgatcatc
     3541 tcgaaggtgg ggattatctg atagccgtcg gctgcgtact cctcgaggaa aggctgcatc
     3601 cgggccaaag tgtcctccgg tccctggtga ccgaggacac ccagccctgt cgtctcgggg
     3661 tgcccgtaga aggcgacata gcgccggggc tggccttcgg ggtagatgag gaagccgcct
     3721 cccggaacct gctggccttt gacgagtacg gcgagctccc aggggtcggc ctcaggtgcg
     3781 gtgcccatgt agcggatcga ctccgcagcc ttgccggcga gggcggcgcc gacttcgggg
     3841 tgaccgagga tgtcagccgg gtcgtaggcg accacgccag ctccgacggc tctaccggtg
     3901 ccagctgctg aaagcagagc caccggattt gccgcgtcca tgagccagat cgtggatgct
     3961 tcggtcgcat gggccaggcc ttcgaggatc tggattggct cgaatgcgcc ctcgggcggg
     4021 accgtcgttg aggacggtga ggtctgaggg agcaccactc gatcccgggc gttgatggcc
     4081 ccaatggtct cgacgatggt cgaggcgtcg ggggtggagg ggagccgaag ctcgtcagcg
     4141 atccccaggg cgagcttggt ctcgtcgacg gcgctgctga tgctgtggtg gaggacggtg
     4201 gcgtcggggg gagtgacgac gctcacgtcg ccaatcaggt ggatccggat cggcttgagt
     4261 cgccccaact cggaagcgag ctgggggtgc gggtgcagga gcggtcctcc gagggcagcg
     4321 gcgagctgtg cggccgcggc cacttcgttg acgtcgtcct caccgacgac aaccacatcg
     4381 tctgcacaca cgaagatctc ccctgacatc gagagggcat ccccggcgat cgacccggtg
     4441 gcgatcgggg tggtgccgcc agtcacccgc acacattccc gattggccgg cgtgctggtc
     4501 gtcggttcgg atgtctgtgt ggtgccgacg ctggtctcgg tcggtgtctc gccaacgatg
     4561 gcactgcacg acacgatcgt cagcgcggcc aagcagaggc ggatggtttt catgggaggg
     4621 ttccggaccg atcggcaggg taaacctggt ccggaatcaa aacagtgttt ccggctccac
     4681 cctctcccga agctccgggc cgttattgcg gaccgagt
//
LOCUS       DBNHXU010000232         3522 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2454246, whole genome shotgun sequence.
ACCESSION   DBNHXU010000232 DBNHXU010000000
VERSION     DBNHXU010000232.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2454246"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="AC161X_05755"
     CDS             complement(<1..857)
                     /locus_tag="AC161X_05755"
                     /inference="COORDINATES: protein motif:HMM:NF013491.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HIR4989091.1"
                     /translation="MPHLFDFSATDGSDPDLLGGKGAGLARMTSLGLPVPPGFTITTE
                     ACRLTMRDGQVPNDLWDEVEAAIARLETSTGRKFGGGPVPILLSVRSGSKFSMPGMMD
                     TILNLGINDEVVRTLIDWSGDAHFAWDAYRRFVQMYGDVVLGVADGLFEEVLTELRAK
                     AGVEDDSELGADDLETATRRFQAIVEEQRPGAMPSDPVEQLHGAISAVFSSWNTKRAI
                     EYRRINAIPDDLGTAANVQMMVFGDLGDDSGTGVCFTRDPGTGARLVYGDYLPRAQGE
                     DVVAGIRNPL"
     gene            1011..1949
                     /locus_tag="AC161X_05760"
     CDS             1011..1949
                     /locus_tag="AC161X_05760"
                     /EC_number="1.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016670 - oxidoreductase activity, acting
                     on a sulfur group of donors, oxygen as acceptor [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HIR4989092.1"
                     /translation="MPTNITDDMVAVPAGTFTMGSDDHYPEERPSHPVSVEAFAVDRH
                     QVTNREFADFVAQTGHVTVAEVPPNPADYPGALPEMLYAGSLVFIQPPGPVDLRDIGN
                     WWSFVEGADWRHPIGPDSDLTDLEDHPVVHVAYADAMAYAEWAGKVLPTEAEWERAAW
                     GGLENAEFAWGDELMPDGQAMANFWQGDFPWQNDLVDGYLRTAPVESFPPNGFGLFDM
                     IGNVWEWTTSWYSTSHPQEVAHACCAPVNPRGAEEEGSFDPSQPEIRIPRKVIKGGSH
                     LCAPNYCRRYRPAARFPEPIDTSTSHLGFRCISRNG"
     gene            1956..2402
                     /locus_tag="AC161X_05765"
     CDS             1956..2402
                     /locus_tag="AC161X_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015299418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family membrane protein"
                     /protein_id="HIR4989093.1"
                     /translation="MKALVARVDELVIPFLRRWGVPTLRISLAVVFIWFGALKVLGVS
                     PVVDLVASTVYWVDPDWFVPALGVVEVLVGVGLAARFGLRLVLLVLALQMLGTFMVFV
                     LLPEITFQDGNPLKLTIEGEFVLKNLVLLAAAMVVGASIDSSEAVT"
     gene            complement(2399..2638)
                     /locus_tag="AC161X_05770"
     CDS             complement(2399..2638)
                     /locus_tag="AC161X_05770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989094.1"
                     /translation="MSKRARERTRQEKQEAKRQKKEDRVEEAALVEAPDETALMEEFR
                     ALSEQYNANVVSESHYNSERHRIFVALGIETEADG"
     gene            3007..3321
                     /locus_tag="AC161X_05775"
     CDS             3007..3321
                     /locus_tag="AC161X_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009382276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA];
                     GO_process: GO:0006397 - mRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA recognition motif domain-containing protein"
                     /protein_id="HIR4989095.1"
                     /translation="MGRMWGVKEVDGSSSGRSMSMSTNIYVGNLSFSTDSAELETLFA
                     PHGRVTKAQVINDRDTGRSRGFGFVEMESAEEAQAAISSLNGKDMGGRQLTVNLAKER
                     SR"
BASE COUNT          581 a         1110 c         1111 g          720 t
ORIGIN      
        1 agtgggttgc ggatcccggc gacgacgtcc tcgccctggg cacgcggcag gtagtcgccg
       61 tagacgaggc gggcgccggt tcccgggtcc ctggtgaagc agacgccggt tcccgagtcg
      121 tcaccgaggt caccgaagac catcatttgc acgttggccg cggtccccag atcgtcgggg
      181 attgcgttga tcctccggta ctcgatggcg cgcttggtgt tccaggagct gaacaccgcg
      241 gatatggcac cgtgcaattg ctcgactggg tcggagggca tggcgccggg cctctgctct
      301 tcaacgatcg cctggaagcg acgcgtcgcc gtctccagat cgtcggcgcc caattccgag
      361 tcgtcctcca cgccggcctt ggctcgaagc tcggtcagga cctcctcgaa aaggccgtcg
      421 gcaaccccga ggaccacatc gccgtacatc tggacaaagc gccggtaggc gtcccaggcg
      481 aaatgagcat ctccggacca gtcgatcagc gtcctgacga cctcatcgtt gatgccaagg
      541 ttgaggatcg tgtccatcat ccccggcatc gagaacttgg acccggatcg gactgatagg
      601 aggattggca ccggcccgcc gccaaacttg cggccggtgc tcgtctcgag gcgggcgatg
      661 gccgcttcga cctcatccca gagatcgttg ggaacctggc cgtcccgcat cgtgagacgg
      721 caggcttcgg tggtgattgt gaaaccggga ggcaccggga gtccaagcga ggtcatcctc
      781 gccagaccgg cccccttgcc acccaatagg tccgggtcgg acccgtcggt ggccgagaag
      841 tcgaagagat ggggcaaagc tcgatgctcc gttcgctgcc ttacgagagg aagttaacga
      901 ctccgactcg atcgacaaca gggtcatacg cgaaacaaac ccgggtcttt cgacctcagt
      961 tgccgagtcc gttatgtttc ggcaggtccg atcgtcgatt ctgaggccac atgccgacca
     1021 acatcacaga tgacatggtt gccgtccccg ccggcacttt caccatgggc tcggacgacc
     1081 actacccgga ggagcgcccg tcccaccctg tcagtgttga agctttcgcg gtcgatcgcc
     1141 atcaggtgac caatcgcgag ttcgccgatt ttgtggccca aaccgggcat gtgaccgtcg
     1201 ccgaggttcc acccaaccct gcggactacc caggggccct tcccgagatg ctctacgccg
     1261 ggtcgctggt attcatacag ccaccgggcc cggtcgacct ccgtgatatc gggaactggt
     1321 ggtccttcgt cgaaggcgca gattggaggc acccgattgg ccctgacagc gatctcaccg
     1381 acctcgagga tcatccggta gtccacgttg cgtacgccga tgcgatggcc tatgccgagt
     1441 gggcgggcaa agtcctcccc accgaggccg agtgggagcg cgccgcatgg ggcgggctcg
     1501 agaacgccga atttgcctgg ggtgacgagc tcatgcccga cgggcaggcc atggccaatt
     1561 tctggcaggg ggactttccc tggcagaacg acctggtcga cggctacctg cggaccgccc
     1621 cggtcgagtc gttccccccg aatggatttg gcctcttcga catgatcggg aacgtctggg
     1681 agtggaccac ttcgtggtac tcgacctcac atcctcaaga ggtggcccac gcctgctgcg
     1741 cccctgtgaa cccccgcggc gctgaggagg agggcagctt cgacccttcg caaccggaga
     1801 tccgcattcc tcgcaaggtg atcaagggcg gctcccatct ctgcgctccg aactactgcc
     1861 ggcgatatcg gcctgcagcc cggttccccg agcccataga cacgtcgacg tcacaccttg
     1921 gttttcgctg tatttcccgg aacggatagt cggacatgaa ggcgcttgtc gcccgggttg
     1981 acgagctggt catcccgttc ctccgtcgat ggggtgtgcc cacgctgcgg atctcgctgg
     2041 ccgtggtgtt tatctggttt ggcgcgctca aggtcttggg ggtgtccccg gtggttgatc
     2101 tggtggcgtc caccgtctac tgggtggacc cggattggtt cgtgccggcc ttgggagtgg
     2161 tggaggtgct ggtaggcgtc ggcctggcag cccggttcgg cctgcgtctc gtgttgttgg
     2221 tgctcgccct tcagatgctg ggcaccttca tggtgttcgt cctgctcccg gagatcacgt
     2281 tccaggatgg caacccgctg aaactgacga tcgagggcga gttcgtgctc aagaatctgg
     2341 tgttgcttgc ggcggcgatg gtggtgggag ccagcatcga ctcttctgaa gcggtgacct
     2401 aaccgtcggc ctcggtttcg atgccgaggg ccacaaagat ccggtgtcgc tccgagttgt
     2461 agtggctttc gctgacgacg ttggcgttgt actgctcgct gagggccctg aactcttcca
     2521 tgagggccgt ttcgtctggg gcttcaacca atgccgcctc ctcgaccctg tcctctttct
     2581 tctggcgctt ggcctcttgc ttctcctggc gggtccgctc gcgggcccgc ttgctcatcg
     2641 cctcacgtcc tctttgtgcc atctggaatc ctctggtcgc gtaaggcgcc aactcaccct
     2701 gtctcagtcg agtacgtcgt ggtgcccgtt accgaagtcc ggtcgaacct ggtccggccg
     2761 cgggtcggcc ctgcgaggcc tgtggtgaat gacgcggtga ccaccctaac cctgtgaggc
     2821 ggttaggccg gaaatgggcc ggtctggctc ccggcgactg ggagtacgtg ttcgcgtcgg
     2881 ctactcttgg aatcacagcg cacgcctgga tgcgtgctgc gccgtttctt cgctccttcg
     2941 taccgtcggg tgaagtcgcc acaggtgcct atctgaccct ccctctcaag tcgaaggtga
     3001 tctcagatgg gtcggatgtg gggcgtcaag gaagtcgacg gttcgagctc tggaaggagc
     3061 atgtcaatgt ccaccaatat ctatgtggga aacctctcgt tttccacgga ctccgctgag
     3121 ttggagaccc tgttcgcgcc ccacggtcgt gtgaccaagg cgcaggtcat caatgaccgt
     3181 gacaccggcc gttcccgcgg tttcgggttc gtcgagatgg aatcagccga agaggctcaa
     3241 gcagcgatct catcgttgaa tggcaaggac atgggcgggc gtcagctcac cgtgaacctg
     3301 gccaaagagc gctcgcgcta agcacccctg gcaaggcggc ttcatgccgc ctccgccatt
     3361 agaacttaag aaaccccccg gaatccgggg ggtttctcac gttctggtga gcccggcggc
     3421 ggaagccgcc cagcccgccg ctactcagcg agcgcgctct ttggccaggt tcacggtgag
     3481 ctgacgcccg cccatgtcct tgccgttcaa agaggagata gc
//
LOCUS       DBNHXU010000233         1936 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1264094, whole genome shotgun sequence.
ACCESSION   DBNHXU010000233 DBNHXU010000000
VERSION     DBNHXU010000233.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1936)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1936
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1264094"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="AC161X_05780"
     CDS             complement(<1..653)
                     /locus_tag="AC161X_05780"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR4989096.1"
                     /translation="MAELSYREAVARGIAQEMRRDPTLVMLGEDIAGAGGVFKTTVGL
                     LEEFGPTRIRDTPISEQAILGAAMGAAMTGVPVIAEIMFSDFLAVCFDMVANEMAKTR
                     YMTGGQFTVPVVIRTANGGGVRFGAQHSQSVENWCMMIPGLKVVAPSTPEDVVGLMAA
                     AVRDPDPVVFFEHKSLMATKSEVPDTEIVGKLGEARIIRAGTDCTIAALAFMVPRAL"
     gene            complement(641..1711)
                     /locus_tag="AC161X_05785"
     CDS             complement(641..1711)
                     /locus_tag="AC161X_05785"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016430067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HIR4989097.1"
                     /translation="MSIEVDAGLYDYAQRPPRLPKATKPENDRLEIDLETRIEMFEMQ
                     QVLRQFEDRVYDLFMQNLVKGTSHLSLGMEAIATGVAKAMRPDDYTFATYRGHAHTVA
                     RGAPLGPVLAELLGRDTGILGGKGGSMHLTSVEHGMMGSYAIIGAHLCVANGAAWSAQ
                     LRGTDQVAVAFFGDGTTNIGAFHEALNLASIWNLPVVFVCENNLYMEYTPIDLVTAAS
                     NPAAGRAPAYDLEQIVIDGNDADEVYVTARVAIEKARRGEGPSLIEAVTYRHGGHSRA
                     DPGKYRPAQEVEAWMAHDPLPTYHQRLLRLGVNESRLSDIVHQVTDRVEEATAFAKAG
                     ERPGEHVLMTDVWADGGSSWRN"
     gene            complement(1701..>1936)
                     /locus_tag="AC161X_05790"
     CDS             complement(1701..>1936)
                     /locus_tag="AC161X_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015664142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR4989098.1"
                     /translation="GHGVGVGLHERPIISRLNSLDDPIEIKEGMYFALETYAPATDGR
                     SAARIEEMMVVTAEGPQITTLFPCEELMVANEY"
BASE COUNT          334 a          637 c          600 g          365 t
ORIGIN      
        1 tccagggctc gaggcaccat gaaggccaac gccgcaatcg tgcagtcggt gccggcgcga
       61 attatccgag cctcgccgag ctttccgacg atctctgtgt ccggaacttc tgacttggtg
      121 gccatcaacg acttgtgctc gaagaagacg accgggtcgg ggtcccgaac tgccgccgcc
      181 atcaggccga cgacgtcctc aggggtggag ggcgccacca ccttgagccc tgggatcatc
      241 atgcaccagt tctcaacgct ttggctgtgc tgggcaccga agcggacacc gcccccgttg
      301 gcggtgcgga tgacaacagg aaccgtgaac tgcccgccgg tcatgtacct cgtcttcgcc
      361 atctcattgg cgaccatgtc gaagcacacc gcaaggaagt cagagaacat gatttcggcg
      421 atcaccggca ccccggtcat ggcagcaccc atggccgcac ccaggatcgc ctgttctgat
      481 atcggggtat cgcggatgcg ggtggggcca aactcctcga gcaggccgac tgtcgtcttg
      541 aacactccac cggcgccggc aatgtcctcc ccgagcatca ccagggtcgg atcgcgccgc
      601 atctcctggg cgattcccct ggcgactgcc tcgcggtagc tcagttccgc caagacgacc
      661 ccccatcggc ccaaacgtcg gtcatcagca catgttctcc cgggcgctcc ccggccttgg
      721 cgaatgcggt cgcttcctcg acgcgatccg tcacctgatg gacgatgtcg gagagcctgg
      781 actcgttgac cccaaggcgg aggagacgct ggtggtaggt aggcagcggg tcgtgcgcca
      841 tccaggcctc gacctcctgt gcggggcggt atttgccggg gtcagcccga ctgtggccac
      901 cgtggcggta ggtgaccgcc tcgatcaggg agggcccttc gcctcgccgt gccttctcga
      961 tcgccaccct cgctgtcacg tacacctcat cggcgtcgtt gccgtcgatc acgatttgct
     1021 cgaggtcgta ggcgggcgcc cgacctgcgg caggattcga agccgccgtc accaaatcga
     1081 ttggcgtgta ctccatatac aggttgttct cacagacgaa gaccacagga aggttccaaa
     1141 tggaggccag gttgagggcc tcatggaagg ctccgatgtt cgtggtgcca tcgccgaaga
     1201 aagccaccgc gacctgatcg gttcccctga gctgcgccga ccacgctgcg ccattggcga
     1261 cacagagatg ggcgccgatg atggcgtagc tgcccatcat cccgtgctcg accgacgtga
     1321 ggtgcatcga accgcccttc cccccgagga tgccggtatc ccttcccaag agctccgcca
     1381 acaccggccc aagtggggcc cccctggcca ccgtgtgggc atggccccga taggtggcga
     1441 acgtgtagtc gtcggggcgc atcgccttgg ccacgccggt ggcgatggcc tccataccca
     1501 gggaaagatg gctggttccc ttcaccaggt tctgcatgaa gaggtcgtac acccgatcct
     1561 cgaactgacg gagcacttgc tgcatctcaa acatctcgat gcgggtttcg aggtcgattt
     1621 cgagacggtc gttttcgggc ttggtcgcct tcgggagccg gggtgggcgc tgggcgtagt
     1681 cgtagagccc ggcgtcgacc tcaatactca ttcgccacca tcaactcctc gcaggggaag
     1741 agtgtggtga tctgtggccc ttcggcagtg accaccatca tctcttcgat ccgcgccgcc
     1801 gaccgcccgt cggtggccgg tgcgtaggtc tcgagggcga agtacatgcc ctccttgatc
     1861 tcgatcgggt cgtcgagtga gttaagcctg ctgatgatcg gccgttcgtg gaggccgacc
     1921 ccaacgccgt ggccga
//
LOCUS       DBNHXU010000234         2738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2653037, whole genome shotgun sequence.
ACCESSION   DBNHXU010000234 DBNHXU010000000
VERSION     DBNHXU010000234.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2653037"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..1630)
                     /locus_tag="AC161X_05795"
     CDS             complement(281..1630)
                     /locus_tag="AC161X_05795"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015304197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4989099.1"
                     /translation="MADQPETRYASSDAGYVAFQVFGEGPISTLFITSWAQNLDVMWD
                     EPGLARYMGRLASFSQVVCFDKRGSGVSDPVPWNTLPSLEEWMDDARMVLDAAGVDQT
                     AVIGDVEGGPMAILFAATYPERVAALVLVNTFARWQRDTDYAIGMPAETLTKLTDAYE
                     EHWGVDADILELTAPTAARDPRFRDWYTRYQRLAMPRGAAAAGYRWVANLDVRMALSA
                     IQAPTLVLTRSDARHHRPTFGRYLAEHISGATYVELPGADTYPFQAGDADAVLDEVEQ
                     FLTGAPAVSPVDRVLSTVLFTDIVGSTQTAARIGDAAWISLRQSHDDLVRENLRKHRG
                     REVSQTGDGFLALFDGPARAVTCAARIIEQLGELGIEIRAGLHTGEIELSGDQVGGLA
                     VHLANRVMAEADPGTILVSATVKDLVLGSGIEFADRGPHQLKGVPDLWTLFEAIRVP"
     gene            complement(1650..2522)
                     /locus_tag="AC161X_05800"
     CDS             complement(1650..2522)
                     /locus_tag="AC161X_05800"
                     /inference="COORDINATES: protein motif:HMM:NF012770.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4989100.1"
                     /translation="MERPDWFIEDLYPFQSHWQEVDGAEIHSLDEGEGPVLLMLHGNP
                     TWSFLYRRMVPYLSDRFRCIAVDYPGFGLSTAAPGYGFTPAEHAITVGRFVDALGVDS
                     FTPVLQDWGGPIGLSVALERKDRVRAVILGNTWAWPRTDAAAERFSAALGEGKTGEFL
                     VDRANVFVNLLVRRAMRRRSPSGREMAMWRGPFPSQSSRLPLRVLARELIGSSEWLTS
                     IEERLPEIADKPSLLFWADQDPVFRGAEKRRWESVLTNRRTYILRHAGHYWQDDAGPE
                     ASLMIRYWWDQSGV"
     gene            complement(2556..>2738)
                     /locus_tag="AC161X_05805"
     CDS             complement(2556..>2738)
                     /locus_tag="AC161X_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006238189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989101.1"
                     /translation="MTRTVIEIVVLAIGWALGGTFGWGTVIFALLVGPLVHFFLPRLT
                     LDPVTDLDEADDHTAN"
BASE COUNT          549 a          871 c          862 g          456 t
ORIGIN      
        1 agtcatgcta cgaggacgag gtccataaat cagctcctac cacaagatgt agtggtgcgg
       61 ggggaagtga ccacgacatc tggtatggac ttcaatactg taattacatc cctgtcatgg
      121 gtcaagcttt tcccgaaaac gtcgtcagaa cgcgaaaatc aacgctctga aggggaagtg
      181 aacggcctcg gtaaccattc aggggaagta gtcggcacct taggccgggc aagcttgggc
      241 ccgccagcca tggccatgaa gagatttcgc gagcgggttg tcaggggacg cgaatcgctt
      301 cgaacaaggt ccagaggtca ggcacgcctt tcagttgatg cgggccccgg tcggcgaatt
      361 cgatccccga gccgagaacc aggtccttga ccgtcgccga caccaggatc gttcccggat
      421 ccgcctccgc catcacccgg ttggcgaggt ggaccgcgag cccacccacc tggtcgccgg
      481 acaactcgat ctcaccggtg tgaagcccgg ctctgatctc gatgccgagc tcacccaatt
      541 gctcgatgat cctcgccgcg caggtgaccg cccgagccgg tccgtcgaac agcgccagga
      601 acccgtcacc cgtctgactc acctcgcggc ctcggtgctt gcgcaaattc tctcggacca
      661 ggtcgtcgtg tgactggcga agcgagatcc aggcggcgtc accgatccgg gcggcggtct
      721 gggtggagcc gacgatgtcg gtgaacagga ccgtgctcag gacgcgatcc accggggaca
      781 cagccggagc tcctgtcagg aactgctcga cttcatccaa gactgcgtcg gcgtctcctg
      841 cctggaacgg gtaggtgtcg gcgccgggta gctccacgta ggtagcaccg gagatgtgtt
      901 cggccagata acgaccgaat gtgggtcggt ggtgcctggc gtcggagcga gtcaacacga
      961 gggtcggagc ctggatcgcc gacaacgcca tgcggacatc gagattcgcc acccaccgat
     1021 accccgcagc ggcggcccca cgaggcattg ccagtcgctg atacctggtg taccaatcgc
     1081 gaaatctcgg atcgcgggca gcagtgggag cggtcagctc caagatgtcg gcgtcgacac
     1141 cccaatgctc ctcgtaggcg tcggtcagct tggtcagggt ttcagcaggc atcccgatcg
     1201 catagtcggt gtccctctgc catcgggcaa aggtgttgac caagacgaga gccgcgaccc
     1261 gctcggggta ggtggcggcg aacaggatcg ccatcggccc gccctcgaca tccccgatca
     1321 cagcggtttg atcgaccccg gccgcgtcca gcaccatcct cgcgtcgtcc atccactctt
     1381 cgagtgaggg caaggtgttc cacgggacgg ggtcggagac gccagacccc ctcttatcga
     1441 agcagacgac ttgactgaat gaagccagcc ggcccatgta ccgggccagc ccgggctcgt
     1501 cccacatcac gtcgaggttt tgggcccaac tggtgatgaa gagcgttgag atcggccctt
     1561 ctccaaacac ctgaaacgcc acgtacccgg catcagagga ggcgtaacgg gtttccggtt
     1621 ggtcggccat cagcggaacc aggggccggt cagaccccgg actggtccca ccagtaccgg
     1681 atcatcaacg aggcttcggg gcccgcgtcg tcttgccagt agtgaccggc gtgacggaga
     1741 atgtaggtgc ggcgattggt caggacactc tcccatcggc gtttctccgc cccccggaac
     1801 accgggtcct ggtccgccca gaacaagagg gatggcttgt cggcgatctc ggggagccgc
     1861 tcctcgatcg acgtcagcca ttcgctggag ccgatcaact ctcgggccag cacccggagc
     1921 gggagacgcg acgactggct ggggaacggc ccacgccaca tcgccatctc ccgacccgat
     1981 gggctgcgac gtcgcatcgc cctcctgacc aagagattga cgaacacgtt cgcccgatcg
     2041 accaggaatt cgccggtctt gccctcaccc aacgcggcgg agaagcgctc ggccgcggcg
     2101 tcggtgcgag gccaggccca ggtgttgccg aggatgacgg ctcgcactcg gtccttgcgc
     2161 tccaaggcga cagagagccc gatcggccca ccccagtcct gcagaacagg agtgaacgag
     2221 tcgacgccga gggcatcgac gaaccgaccg acagtgatgg cgtgctcggc tggagtgaag
     2281 ccataccccg gagccgcagt cgaaagcccg aagcccggat agtccacggc gatgcagcga
     2341 aatcggtcgg acaggtaggg caccatccgt cggtagagga acgaccaggt gggattgccg
     2401 tggagcatca ggagcacagg tccctcaccc tcatcgagag agtggatctc tgctccgtcg
     2461 acctcctgcc aatgactctg gaatggatag aggtcttcga tgaaccagtc aggtcgctcc
     2521 acgatgcggc aacctactcg ttgttcgctg ccacttcagt tggcagtgtg gtcgtcggcc
     2581 tcgtcgagat cggtcaccgg gtccagggtc agcctgggca ggaagaagtg gaccagaggg
     2641 ccgacaagca aagcgaagat cacagtgccc catccgaagg ttccccccag ggcccagcca
     2701 atggcaagga ccacgatctc gatcacagtt cgggtcat
//
LOCUS       DBNHXU010000235         7263 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1661280, whole genome shotgun sequence.
ACCESSION   DBNHXU010000235 DBNHXU010000000
VERSION     DBNHXU010000235.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7263)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7263)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7263
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1661280"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..689
                     /locus_tag="AC161X_05810"
     CDS             <1..689
                     /locus_tag="AC161X_05810"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR4989102.1"
                     /translation="VALAEAADSEPTRVVILAGAGRSFCAGYDLSEAGGETDIEVGLG
                     RSLDRLLELFDHPKPIIAQIQGHCLAGGCDLMMMCDLAVAADDAVFGQPEIRFGAGVV
                     AYVMPWLVGARRAKELLFTGIDKLPADEAERIGLVNKVVPRDQLEDETMRLAEALAVV
                     DPFAMQMTKRAINWAWESAGFRDALRGGLELGAMIETTRVPEREEFERIAAERGLAEA
                     IRWRDERFES"
     gene            686..1531
                     /gene="purU"
                     /locus_tag="AC161X_05815"
     CDS             686..1531
                     /gene="purU"
                     /locus_tag="AC161X_05815"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HIR4989103.1"
                     /translation="MRRYVLTLSCPDRSGIVNAVTGALLEIEANILESAQFGDVDSST
                     FALRIVFETEQEDPTVVEAPLAKVSNELSGALKLRAIDARPRALVMVSKYDHCLLDLL
                     HRHRHGELEIEIPIIVSNHTDCAELASHYGIPYVHIPVTEETKTDAESRLMTLIEEHS
                     IDFVVLARYMQILSPETCKALEGRAINIHHSFLPGFKGSRPYHQAHQRGVKLIGATAH
                     YVTVDLDEGPIIEQDVARVTHAHTAEDLVSIGRDVERMVLARAVRYHAEERVILIGSK
                     TVVFA"
     gene            complement(1550..2338)
                     /locus_tag="AC161X_05820"
     CDS             complement(1550..2338)
                     /locus_tag="AC161X_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020395056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989104.1"
                     /translation="MRTKASLGLKLAAGFTLAFLHIPILFVFLYAFTTESASFRFPPP
                     GFTTEWFGVDWQNSDIRAALGLSLRVAAISTLVALVVGTLAAAAVWRTNFFGKNTLSL
                     FIILPIALPGIITGIALRSAINLGNFSFSFWTLVIGHATFCVAVAFNNVIARMRRTSP
                     NILEASMDLGARGWQTFRHVLLPQLAPAMVVGGMLAFALSFDEIIVTTFTAGNQQTLP
                     IWIFSSLVRPRNRPVTNVVAVFVILITFIPIIFAQRFAQRAEES"
     gene            complement(2335..3267)
                     /locus_tag="AC161X_05825"
     CDS             complement(2335..3267)
                     /locus_tag="AC161X_05825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014321175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989105.1"
                     /translation="MATDTKPPPLTHRIARGLHRRPWLKVLLLLALPLIWMVGVYGGS
                     LGALLVQSFYALDTFSGAIGDSLTLDSWRQLFTEANVAVMVRTVGMAAAVTVASVVIA
                     FPLAYFMARYARKQVKAVLVLAVLVPLWSSYLVRVYSWKLILAADGIINWFVERLGLG
                     RALEFLLDTPVIGGPSLSISLLGQFVVFVYIWLPYMILPIHASLERVPGSLLEASADL
                     GAQPRTTFRKVTWPMAIPGVAAGSIFTFSLTLGDYIIPTIIGDSSPFIGLAIYSYQGV
                     AGNLPLAAAFSFIPIAVMAVYLTVARRFGAFEAL"
     gene            complement(3326..4513)
                     /locus_tag="AC161X_05830"
     CDS             complement(3326..4513)
                     /locus_tag="AC161X_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4989106.1"
                     /translation="MRSRKFLFLITAMAVLATACTDSGDEEGADLPTEIGDGEGKVVI
                     VSWAGYIERGETDPAYDWVTEFEAETGCMVENKTAGTSDEMVALMTGSSEYDLVTASG
                     DSSLRLIAGGAVQEINPDLIESWDTIDPRLQDAEWHTINGKHYGTPYAEGFNVLGYNT
                     EVFGETPPDSWSVVFEETILPDGASNKGRVQAYDGPIYIADAALYLSATQPDLGIEDP
                     YQLNEDQYQAALDLLRVQRTLVGRYWHDAFIQIDDFTNEGVAASSTWPFQTNVLQFAG
                     EPIDKVVPVEGATGWADTTMMSSNAPHPNCSYMWMEHSLNPKLQGDLASWFGTVPAVP
                     AACEGNELLGPDGCANNGSESFDEIQFWKTPTDDCNGTADIGTCVPYYRWVTDYLAVI
                     GGR"
     gene            complement(4510..5598)
                     /locus_tag="AC161X_05835"
     CDS             complement(4510..5598)
                     /locus_tag="AC161X_05835"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017987038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989107.1"
                     /translation="MSLDKVTKMFGEVPAVNGVTLDIADGEFFSMLGPSGSGKTTCLR
                     MIAGFEIPSSGRVSLHGNDVTGVPPYDRDVNTVFQDYALFPHMTVAENVGYGPMVRKE
                     PDQGDRVSKALAMVHLNGFADRKPSQLSGGQRQRVALARALINRPSVLLLDEPLGALD
                     LKLRQAMQLELKTLQREVGITFIYVTHDQEEALTMSDRLAVFNHGLVEQVGTPSDVYE
                     NPRNAFVAGFVGTSNVMSGPLAERIMGSPSPFTVRPEKISIDEPASPVPDGMAAVDGT
                     ISEAVYLGMYTRYRVDVGGSMVEVAAQNTAADHSDAQRQIGRSVRLTWTKDSCRPLEE
                     TPREAEPDEMAPALAGAGNNTSEEETIE"
     gene            5708..6001
                     /locus_tag="AC161X_05840"
     CDS             5708..6001
                     /locus_tag="AC161X_05840"
                     /inference="COORDINATES: protein motif:HMM:NF020317.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HIR4989108.1"
                     /translation="MAKFIILGNYTAQGIGAIKDAPSRLDATRDRMAAFGVTIEDFFL
                     TFGEYDIVSVIDAPDSASAAKALMTLGMAGNVGTVTMAALTESEFRKAVSELP"
     gene            complement(6086..>7263)
                     /locus_tag="AC161X_05845"
     CDS             complement(6086..>7263)
                     /locus_tag="AC161X_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015306360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HIR4989109.1"
                     /translation="AENIEYVVTPAVVVWFAGAYAQPVFLELFNVDIAQRWWWLIFYV
                     IFVGLNILGIEATMRFSVAICIISLAVLAVFFAFAIPEFDASRLTNIVADAGQSSFLP
                     FGTAGIFAALPFAIWFYLAIEELPLAAEESMDPKRDVPRGTMWGLWTLVIAAMGVLFL
                     NTGVTGAEEIRTAGEPLLDGFRVVLGSETNAAILGLFALTGLIASFHTIIYAYGRNIF
                     SLSRAGYFPHWMSITHGKRKTPYVALLLGGVVGYGLVWLLDSQSSGTAGAALLSMAVF
                     GAVISYFMQMVSFIRLRSKMPNIERPYRSPVGVFGAGLAAVIALVSLAALFLNDAYLP
                     GVYGVAIWFVLGILYFGLSGRNKLVLSPEEEFAMTMGEHGHPEEEGYGTTAVADTTS"
BASE COUNT         1467 a         2151 c         2315 g         1330 t
ORIGIN      
        1 cggtggcgtt ggcggaggca gcggacagtg agccgactcg ggtggtgatc cttgccggtg
       61 ccggacgctc cttctgcgcc ggttacgacc tttccgaagc agggggcgag accgacatcg
      121 aggtcggcct gggccgaagt ctcgatcgcc ttctcgagct gttcgaccat cccaagccaa
      181 tcatcgccca gatccagggg cactgcctgg cgggcggatg cgatctgatg atgatgtgtg
      241 atctcgcagt cgccgcggac gatgccgtct tcgggcagcc cgagatccgg ttcggagcgg
      301 gtgtggttgc ctatgtgatg ccatggctgg tcggcgcacg acgggcaaag gagctgttgt
      361 tcaccggaat cgacaagctt cccgccgacg aggcagagcg gattgggttg gtgaacaagg
      421 tcgtcccgag agatcagctg gaggacgaga cgatgcgact tgctgaagcc cttgctgtag
      481 tcgacccatt cgccatgcaa atgaccaaac gggccatcaa ctgggcttgg gaatccgcgg
      541 gatttaggga cgcgctccgt ggtggcctcg aattgggtgc gatgatcgag acgacgcgag
      601 tgccggagag ggaagaattc gagaggatcg ctgccgagag gggcttggct gaagcgattc
      661 gatggcgaga cgagaggttt gagagttgag gcgttacgtg ttgaccctga gctgtcctga
      721 ccgttccggg atcgtcaacg ccgtcaccgg tgctctcctc gagatcgagg ccaacattct
      781 cgagagcgct caattcggcg acgtcgacag cagcacattc gccttgcgta ttgttttcga
      841 aaccgagcag gaagacccga cggtcgtcga agcccctctg gcaaaggtct ccaacgagct
      901 atccggggcg ctcaagttga gggctatcga cgccagaccc cgggccctcg tcatggtctc
      961 gaaatacgac cattgtctgc tcgacctcct ccatcgccac cgacatggag aactggagat
     1021 cgagatcccg atcatcgtgt ccaatcacac agattgtgcc gagctggcca gtcactacgg
     1081 gatcccatac gtgcatatcc cggtcacgga ggagaccaag accgatgccg agagtcgatt
     1141 gatgacgctc atcgaggagc actcgatcga cttcgtggtc ctcgcccgat acatgcagat
     1201 cctgtcaccg gagacgtgca aggccctgga ggggcgagcc atcaacatcc accattcttt
     1261 cctgcccggt ttcaagggat cacgccctta tcaccaggct catcagcgag gcgtcaagct
     1321 catcggggcc accgctcact acgtgaccgt cgacctcgac gaagggccga tcatcgaaca
     1381 ggatgtggcg agagtcaccc acgctcacac ggcggaggac ttggtctcga taggccgcga
     1441 cgtcgagcgg atggtcctcg cccgtgccgt ccgctatcac gccgaggagc gggtgatctt
     1501 gatcgggtcg aagaccgtcg tttttgcctg agtagccctg ccccgagcct cagctctctt
     1561 cggcccgttg ggcgaaccgc tgggcgaaga tgatcggtat gaaggtgatg aggatcacga
     1621 aaaccgccac cacgttggtg accgggcggt ttcggggccg taccagagac gaaaaaatcc
     1681 agatcggaag cgtctgctga ttcccggcag tgaacgtggt gacgatgatc tcgtcgaagg
     1741 agagagcgaa ggcgagcatc cctcccacga ccatggcggg ggcgagttgg ggcaggagta
     1801 catgacggaa ggtctgccag cctcgcgccc caaggtccat tgacgcttcg aggatgttgg
     1861 gcgaggtccg ccgcattcgg gcgatgacgt tgttgaacgc caccgcaaca cagaacgtgg
     1921 cgtgcccgat caccagagtc cagaacgaga acgagaagtt cccgaggttg atggccgagc
     1981 gaagagcgat ccccgtgatg atcccgggga gggcgatggg aaggatgatg aacaggctca
     2041 gtgtgttctt gccgaagaag ttggtccgcc ataccgcggc ggcggcgaga gtccccacta
     2101 ccagggccac caatgtcgag atcgcggcca cccgcagcga aaggcccaag gctgccctga
     2161 tgtccgagtt ctgccagtcc acgccgaacc attcagttgt gaaccccgga ggaggaaagc
     2221 ggaagctggc cgactcggta gtgaaggcat acaggaacac gaacaggatc gggatgtgga
     2281 gaaaggcgag tgtgaacccc gctgccagct tcagcccgag gcttgccttc gttctcatag
     2341 cgcctcgaaa gcgccgaaac gacgggcaac tgtcaggtaa accgccatca ccgcgatagg
     2401 tatgaaggag aaggccgcgg cgagggggag gttgccggcc acaccctgat aggagtagat
     2461 ggcgagaccg atgaatgggc tggaatcccc aatgatggtg gggatgatgt agtcgcccag
     2521 ggtcagggag aaggtgaaga tcgacccggc ggccacgcct gggatggcca tcggccacgt
     2581 caccttgcgg aacgtcgttc gcggttgggc accaaggtca gcagatgcct cgagcaagga
     2641 cccgggcact cgttccaggg aggcgtggat ggggaggatc atgtagggca gccagatgta
     2701 gacgaagacc acgaactgcc cgaggagaga aatcgagagt gaaggtccgc cgatcacggg
     2761 ggtgtcgagc aggaactcga gggcgcgtcc caggcccaac ctctcgacga accaattgat
     2821 gatcccatcg gcggccagga tcagcttcca ggagtagacc cggaccagat atgaggacca
     2881 gagcggcacc agcaccgcca agacgaggac ggctttgacc tgtttccggg cgtacctcgc
     2941 catgaaatag gcgagtggga aggcgatgac caccgacgcc acggtgaccg cggcagccat
     3001 gcccacggtc ctcaccatca cggcgacgtt cgcttcggtg aacagctgtc tccacgagtc
     3061 gagggtcagg ctgtccccga tcgccccgct gaatgtgtcc agggcgtaga agctctggac
     3121 gaggagggcg ccaagactcc cgccgtatac ccccaccatc cagatcagcg gaagggcaag
     3181 gagcaagagg accttcagcc aaggtcgtcg gtgaagccct ctcgcgatcc gatgagtcag
     3241 cgggggtggt ttggtgtccg tagccattgt gggtgcccgg atgtgggtgg cgccccggcg
     3301 ccacccacat caacgggcct atcgctcagc gaccgccgat cacggccaga tagtcggtga
     3361 cccatcggta atagggaaca caggttccaa tgtccgcggt cccgttgcag tcgtcggtcg
     3421 gggtcttcca gaactggatc tcgtcgaatg actcgcttcc gttgttggcg cagccgtcgg
     3481 ggccgagcaa ctcgttcccc tcacaggcag cggggaccgc cgggacggtg ccgaaccagg
     3541 acgccaggtc gccctgaagc ttgggattca gcgagtgctc catccacatg taggagcagt
     3601 tggggtgcgg agcgttcgag ctcatcatcg tggtgtcggc ccatccggtt gctccctcga
     3661 ccgggaccac cttgtcgatc ggctcaccgg cgaactgcaa cacgttggtc tggaacggcc
     3721 aggtagatga ggcggcgact ccttcgttgg tgaagtcgtc gatctgaatg aaggcgtcgt
     3781 gccagtacct acccaccagc gtgcgctgga cgcgcaagag atcgagagca gcttggtatt
     3841 ggtcttcatt cagctggtag gggtcctcaa tcccgagatc gggctgggtg gcgctcaggt
     3901 agagcgctgc gtccgcgata tagatcgggc cgtcgtaggc ctgaactcgc cccttgttcg
     3961 aggcgccatc gggcagaatc gtctcctcga agacaacgct ccacgaatcc ggcggggtct
     4021 ccccgaacac ctcggtgttg tagccgagca cgttgaatcc ctcggcatat ggcgtcccgt
     4081 agtgcttgcc gttgatggtg tgccattcgg cgtcctggag acgggggtcg attgtgtccc
     4141 agctttcgat gaggtcgggg ttgatctctt gaaccgcccc gccggcgata agcctgaggc
     4201 tcgagtcccc tgaagcggta accaggtcgt attcgctgga cccagtcatc agcgccacca
     4261 tttcgtccga ggtgcctgcg gtcttgttct cgaccatgca gccggtctcg gcctcgaact
     4321 cggtgaccca gtcgtatgcg gggtcggttt cgccccgctc gatgtagccg gcccaggaga
     4381 cgatgaccac cttgccctca ccgtcaccga tttcggtggg aaggtccgcg ccttcttcgt
     4441 cacctgaatc ggtacaggca gtggcaagca ccgccatcgc cgtgatcagg aaaagaaact
     4501 tccttgatct cattcgattg tttcctcctc tgaggtgttg tttccggctc cggcaagggc
     4561 cggggccatt tcatccggct cagcctctcg aggggtttcc tcgaggggcc ggcacgaatc
     4621 cttggtccat gtcagccgca ccgatcggcc aatctggcgc tgggcatcac tgtgatcggc
     4681 tgccgtgttc tgagcagcga cctcgaccat ggagccgccg acatcgacgc gatacctggt
     4741 gtacatcccg aggtagacag cctcggagat tgtcccgtca acggcagcca ttccgtcagg
     4801 caccgggctc gccggctcgt cgatgctgat cttctcgggg cggacggtga acggggaggg
     4861 tgaccccatg atccgttccg ccaacggccc cgacatgaca ttcgaagtcc cgacgaatcc
     4921 ggcgacgaag gcgttgcggg ggttctcgta gacatccgat ggagtgccca cctgttcgac
     4981 gaggccgtgg ttgaagacgg cgaggcggtc gctcatggtc agggcctcct cttggtcgtg
     5041 ggtcacatag atgaaggtga tccccacctc gcgctgcagg gttttcaatt ccaactgcat
     5101 cgcctgacgc agcttgagat ccagggcacc gagcggttcg tcgaggagaa gtacggaagg
     5161 ccgattgatg agggctcggg ccagcgcaac gcgttggcgc tgcccgcccg agagctgact
     5221 cggtttgcgg tcagcgaagc cgttgagatg gaccatggcc agggccttgc taacccggtc
     5281 cccctgatcg ggctctttgc gaaccatcgg cccgtagccg acgttctcgg caacggtcat
     5341 atgggggaag agtgcgtagt cctgaaagac ggtgttcacg tcgcggtcat agggcggcac
     5401 accggtcacg tcgttgccgt ggagggaaac gcgacccgag ctcgggattt cgaagccggc
     5461 gatcatgcgc aagcaggttg tctttccgct tccggacggg ccgagcatcg aaaagaattc
     5521 cccgtcggct atgtcaaggg tcacaccgtt gaccgcggga acctcgccga acatcttcgt
     5581 tactttgtcg agactcacgg ccgcggccaa ttagatcctg acctcccaat cgaggccgga
     5641 gcctatcggc acgtgaacaa tcctgtcggg ggagtaggtt cgatgagttg gctgacaaag
     5701 gagcgatatg gccaaattca tcatccttgg caactacacg gcgcagggaa tcggagcgat
     5761 caaggacgct ccctcccggc tagacgctac gagggatcgc atggcggcat ttggtgtgac
     5821 catcgaggac ttcttcctga cctttggtga gtacgacatc gtgtcggtga tcgatgctcc
     5881 ggatagcgcg agtgcggcaa aggcactgat gactctcggg atggcaggga acgtggggac
     5941 ggtaacgatg gctgccctga ccgagtctga attcaggaag gccgtatccg aacttccgtg
     6001 atcggcacga aagatctggt tcctcctccg gatggaaaat gggccaccgc tcagcggtgg
     6061 cccattttca tcatcagcct gtaggtcagc tcgtggtgtc ggccaccgcg gtggtgccgt
     6121 atccctcttc ctcggggtgg ccgtgctcgc ccatggtcat tgcgaattcc tcctcaggcg
     6181 agagaaccag cttgtttcgg ccggacagcc cgaaatagag gatcccgagc acgaaccaga
     6241 tggcgactcc gtatacccca ggcaggtagg catcgttcaa gaagagcgcc gccaacgaga
     6301 ccagagcaat gaccgccgcc agtccggctc cgaacacacc gaccgggctc cggtatggcc
     6361 gctcgatgtt cggcatcttg ctccgcaatc tgatgaacga gaccatctgc atgaagtagg
     6421 agatgaccgc accgaacaca gccatgctca gcagcgccgc tccggccgtg cccgatgact
     6481 gactgtcgag gagccatacc aacccatacc cgactacccc acccagtagc aaggccacgt
     6541 acggcgtctt ccgcttgccg tgggtgatcg acatccagtg tgggaaatac cctgctctcg
     6601 acagcgagaa gatgttcctc ccgtaggcgt agatgatggt gtggaagctg gcgatcaacc
     6661 cggtcagtgc gaacagacca aggatggcgg cgttggtttc gctgccgaga accacccgga
     6721 acccgtcgag caatggctca cctgcggtcc ggatctcctc agctccggtg acgccggtgt
     6781 tgaggaagag gacccccatc gcggcgatca ccagggtcca caatccccac atcgtgcctc
     6841 ggggcacgtc acgcttggga tccatcgatt cctcagctgc cagtggtaat tcctcgatgg
     6901 cgaggtagaa ccagatggca aacggtaggg cggcgaatat ccccgccgtg ccgaacggca
     6961 ggaacgagct ctggcccgcg tcggccacga tgttcgtcag cctcgaagcg tcgaattcgg
     7021 ggatggcgaa tgcaaagaac accgcgagga ctgccaggga gatgatgcag atcgccaccg
     7081 agaatcgcat cgtggcctcg atcccgagga tgttcagccc gacgaagatc acgtagaaga
     7141 tcagccacca ccagcgctgg gctatgtcca cgttgaacaa ctccaagaac accggttggg
     7201 cgtaggcacc ggcgaaccag accaccactg ctggagtcac cacgtattcg atgttctccg
     7261 cca
//
LOCUS       DBNHXU010000236         4810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3051373, whole genome shotgun sequence.
ACCESSION   DBNHXU010000236 DBNHXU010000000
VERSION     DBNHXU010000236.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3051373"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="AC161X_05850"
     CDS             complement(<1..231)
                     /locus_tag="AC161X_05850"
                     /inference="COORDINATES: protein motif:HMM:NF013629.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4989110.1"
                     /translation="MTSTIDHDIEARLGAAGVRYTRARRAVVDALARADGPRSASELH
                     DDMDRDVPLSSLYRSLSVLDEAGVLEPHNSLKG"
     gene            301..1209
                     /locus_tag="AC161X_05855"
     CDS             301..1209
                     /locus_tag="AC161X_05855"
                     /inference="COORDINATES: protein motif:HMM:NF013465.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter solute-binding protein,
                     Zn/Mn family"
                     /protein_id="HIR4989111.1"
                     /translation="MKLPHRAFLVMLCLIAVGCAPTSNPDSQGPLVVATTTVLGDLAS
                     AIVGDQATVETLMPAGADPHDFQPSSQQVALINGADLVIANGLGLEEGFIDVLDAAKA
                     DGVNLLEVGPLIDPIPFAEGGPEELGDDPHFWNDPVRAATGADVIADALSEVDPSVDW
                     SSQATDYGNELRDLASEIEVMLQGIPAADRKLVTNHDSLGYFADRYGFEILGVVIPGG
                     STLAEPSSAELANLVQVMREQGVSVIFAETTSPSVLADAIAAELGADVEVVELYTDSL
                     GQPGSGAETLLDMLRLDAERIAGALS"
     gene            1238..2077
                     /gene="aztB"
                     /locus_tag="AC161X_05860"
     CDS             1238..2077
                     /gene="aztB"
                     /locus_tag="AC161X_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003997240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter permease AztB"
                     /protein_id="HIR4989112.1"
                     /translation="MDWLTEPFALAFQQRALIGGALAALALAVVGTWVVIRGMTFLGD
                     ALVHGVIPGIALAILLDFNVLLGAALAATVMIAGINLVHRQTSFSEDTGIGLLFVGML
                     GLGVILISRSDSYTGSLTGILFGDALGVTGEDLVVLGVVAGIVLLVSTLLYRNFLVLS
                     FNEHKAKLLGLNPRLAHLALLGLTTLSIVGSFQTVGTLLVFGLLVGPAATAALLVKKV
                     PAMMMTAAAIGILSVALGLVISYYADTSGSATISVVPIVLFFIVLTIKSLTRRAPALA
                     SHG"
     gene            complement(2074..2702)
                     /locus_tag="AC161X_05865"
                     /pseudo
     CDS             complement(2074..2702)
                     /locus_tag="AC161X_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002926109.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
     gene            complement(2726..3631)
                     /locus_tag="AC161X_05870"
     CDS             complement(2726..3631)
                     /locus_tag="AC161X_05870"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR4989113.1"
                     /translation="MPETLKVGVQLPEVEREAGWLEIRDVARTAEEVGLDSIWFGDHL
                     LYRDDTFGVRGPLETWAILAALGEATERVSLGPLVACTSFHAPAMLAKKAATVDEISG
                     GRLILGLGAGWNQTEYDGFGFPFDHRIDRFEEAFTIIRTLLSEGHVDFEGTYYTVRGA
                     ELAPRARPGIPLLVGSNGPRMLAITLPYVSMWNSWHASFGNQLSGLAPLLEEIDHAAA
                     GAGREPGEVEKTVSIYIQLPGGTGRITGRGVLLDDVPHTGSREDLAQLLRDYAAIGIS
                     HVQLVLDPIDSASVEEMGEVIRLVD"
     gene            3753..>4810
                     /locus_tag="AC161X_05875"
     CDS             3753..>4810
                     /locus_tag="AC161X_05875"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase/oxidase"
                     /protein_id="HIR4989114.1"
                     /translation="MRSETFDIAVIGGGVTGNGIALDAATRGMSVALIEQRDYASGTS
                     SRSSKLFHGGLRYLEHRNFTLVKEALAERNLMLNRLCPHLCTPVSFLYPLQHLFWERL
                     YVGAGVFLYDRLAARANNPLPQHRHLSQRSALEMAPALAEASLKGAVLYWDVMVDDAR
                     HTMTLARTARSHGAALAPSTRATGLIIENGSVAGVQARDLESGTDIEIHARKVIAAGG
                     VWSDDIQAMAGDRGQDVSASKGVHLVVPRDRINSSTGMILRTEISVLFVIPWGRHWLI
                     GTTDTPWSLRRAHPAACLDDIQYLLRWVNTALAVPIGPEDVVGVYAGLRPLLAGESDE
                     TSKLSREHAVIVSDTGLIT"
BASE COUNT          835 a         1583 c         1501 g          891 t
ORIGIN      
        1 ccccttgagg ctgttgtgcg gctcgaggac acccgcttcg tcgaggacgc tgaggcttcg
       61 atagagcgag gacaacggca catcccgatc catgtcgtcg tgaagctcgg aggccgatcg
      121 cggcccatcg gctcgcgcca gggcatcgac gacggcacgg cgagcccgtg tgtaacggac
      181 gcctgccgcc ccgagccggg cttcgatgtc gtggtcgatg gtgctggtca tcggattgga
      241 gcccaatcat aacctctggt atcttgataa tgattctcag tttcggtaag gagacgggtc
      301 atgaagctgc cccatcgagc cttcctggtc atgctttgcc tgatcgcggt tgggtgcgcc
      361 cccacctcca acccagactc tcaaggtcct ttggtggtgg ccaccacaac cgtcctcgga
      421 gacctggcct cggccatcgt cggggatcaa gcgaccgtgg agacgttgat gcccgcgggc
      481 gccgaccccc atgactttca gccctcgtca cagcaggtgg ccttgatcaa tggcgccgac
      541 ctcgtgatcg ccaacgggct tggcctcgag gaagggttca tcgacgtcct cgatgccgcc
      601 aaggccgatg gggtaaacct cctcgaggtc ggtccgctga tcgatccgat cccgttcgcc
      661 gagggtggcc ccgaagagct cggggacgat ccccacttct ggaacgaccc ggtccgggcc
      721 gccaccggcg ccgacgtgat cgccgacgcc ttgtcagaag tcgacccttc cgtcgattgg
      781 tcgagtcagg ccaccgacta cggcaacgag ttgcgggatc tggccagtga gatcgaagtc
      841 atgctccaag ggatccccgc tgccgatcgc aagctggtga ccaatcacga ttccctgggc
      901 tatttcgccg atcggtacgg gttcgagatc ctcggtgtcg tcattcccgg tggctccacc
      961 ctcgccgagc cgagctccgc cgaattggcg aacctggtcc aggtgatgcg agaacagggc
     1021 gtctctgtga tcttcgccga aaccacctcg ccgtccgtcc tcgcagacgc gatcgccgcc
     1081 gagctcggag ctgatgtgga ggtagtcgag ctctacaccg actccctcgg ccaacccggc
     1141 tctggcgcgg agaccctctt ggacatgctc cgcctcgacg cagagcggat cgcgggcgcc
     1201 ctgagttgac aaccatccca ctacgttgcg cctggccatg gactggctga ccgagccctt
     1261 cgccctcgcc ttccagcaaa gggcactgat cgggggcgct cttgctgcgc tagccctggc
     1321 cgtggtcggg acctgggtgg tgatccgggg gatgaccttc ctcggcgatg ccctggttca
     1381 cggcgtgatc cctggaatcg ccctcgccat cctgctcgac ttcaacgtgc tcctcggagc
     1441 cgcgctggcg gcgacggtga tgatcgccgg tatcaacctg gtccatcgcc agaccagctt
     1501 ctccgaggac accggcatcg gccttctctt cgtcgggatg ctcgggctgg gcgtgatcct
     1561 catctcacgc agcgactcct acaccggcag ccttaccgga atcctctttg gcgacgccct
     1621 tggagtcacg ggtgaggact tggtcgtcct cggagttgtg gcagggatcg tgctcttggt
     1681 ctcgaccttg ctctaccgaa actttctggt cctgtccttc aatgagcaca aggcgaagct
     1741 gctcggactc aacccgcggc tggcgcatct cgcccttctt gggttgacga cgctgtcgat
     1801 tgtggggtcg ttccagaccg tggggacact cctggtgttc ggccttctgg tcggtcccgc
     1861 ggcgaccgca gcgctgctgg tcaagaaggt gccggcgatg atgatgaccg ctgcagccat
     1921 cggcatcctc tcagtcgctc tgggcctcgt catcagctat tacgcggata cctcgggctc
     1981 ggcaacgatc tctgtggttc cgatcgtgct cttcttcata gtgctgacga tcaagtcgct
     2041 aacgcggaga gcacccgcct tggccagcca cggctagccg cgaaggcgcg gattctgggt
     2101 gtaggtcccg gagacagagg cctcggcaag aacatgaccg gcgatggcat cccgaacctg
     2161 ctgaccggtg aaatcacccg tcaccgccac ctcatcgatg tcgagggcat acagcgtgaa
     2221 tatgtagcga tgcgggatcg agtcgttcca gggcggtgcc ggaccgtcat agccgaggta
     2281 gttccccctc atttcggggt cgccggcgaa ccaccctgtg tagtcattga gaccttgccg
     2341 gcacccgcca ggcccgaagg cgacgttctt gccgtggggc atgacgccca cacagaactc
     2401 tccctcgcca atcgagcgca acccgggggg caactccacg gtcacccagt ggaagaagtc
     2461 gacccggggc agatccgcgg ggacctcccg gtcttcttga ttcacgtcgt cgccgcggga
     2521 agggcaatcg gggtcgtggc agatgagggc gaaggatctc gtcccgtccg gcacctcgcc
     2581 cagctcagct cgggattgac gttctcggag aatgtgacat gggtctcggg atgtgggatc
     2641 gcaagcgcgt accgatcggg gatcgggcca ccgttgcgaa aactggctgt tgtcagcttc
     2701 atggcacctc cggaatcgac actaatcaat cgaccaggcg gatcacctcg cccatctcct
     2761 cgacactggc cgagtcgatc gggtcgagca ccaactggac atggctgatc ccgatggcgg
     2821 cgtaatccct caacagctga gcgagatcct cccgacttcc ggtgtgaggg acgtcgtcta
     2881 gaagcactcc acgcccggtg attcggccgg tgccacccgg aagttggata tagatgctga
     2941 cggtcttctc gacctccccg ggctcacgac cggcgccggc cgcggcatgg tcgatctcct
     3001 ctagaagagg ggcgagcccg ctcaattgat tgccaaagga ggcatgccac gagttccaca
     3061 tcgacacgta agggagggtg atagccagca tccgaggccc gttggacccg accagcaacg
     3121 ggatgcccgg tctcgccctg ggtgcgagct cggcgccgcg aaccgtgtaa taggtgcctt
     3181 cgaaatcgac gtggccttcg ctgagcagag tgcgaatgat ggtgaaggcc tcttcgaacc
     3241 gatcgatgcg atgatcgaag ggaaagccga agccgtcgta ctcggtctgg ttccaccccg
     3301 ccccgagacc caggatcaac ctcccgccgg agatctcatc gacggtggcg gccttcttgg
     3361 ccagcattgc cggggcgtga aacgaggtgc aggcgaccag cgggcccagg ctcactctct
     3421 ccgtggcctc gcccaaggct gccaggatgg cccacgtctc caacggcccc cgcacaccaa
     3481 aggtgtcatc ccggtagagg agatggtcgc cgaaccagat cgagtcgagg cctacctcct
     3541 cagcagtgcg cgccacgtcc cggatctcga gccatcccgc ctctcgctcg acttccggaa
     3601 gctggacccc aaccttgagt gtctctggca tggcggcatc gtacggtgcg ctccgctatc
     3661 ggtgtttgcg ggctaaacct ggggtggtcg tgacccacgc gaggcccaca tcccagctca
     3721 actggcggca acggtcgctc gatctcaagc gaatgcgctc ggagaccttc gacatcgcgg
     3781 tgatcggtgg cggggtgacc ggcaacggga tcgctctgga tgccgcgact cgcgggatgt
     3841 cggtggccct gatcgagcag cgggactacg cctcggggac gtcgagtcgc tcgagcaagc
     3901 tgttccacgg cggcctccgc tatttggagc atcgcaactt caccctggtc aaggaggcgc
     3961 tggccgagcg aaacctgatg ctgaaccggc tttgccccca cctttgcacg ccggtctcct
     4021 tcctctaccc gttgcaacat ctgttctggg agcggctata tgtcggcgcc ggagtcttcc
     4081 tctacgacag gctcgcggct cgagccaaca acccgctccc ccagcaccga catttgagcc
     4141 aacggagcgc cctcgaaatg gcgccggctc tggcagaagc ctcgctcaag ggcgcagtcc
     4201 tctactggga cgtcatggtc gacgacgccc gtcacacaat gacgctggcc cgcactgccc
     4261 ggtcacacgg ggccgctctc gccccgagca caagagccac cgggttgatc atcgagaacg
     4321 gctcggtagc cggagtccag gcacgcgacc tcgagtcggg cacagacatc gaaatccatg
     4381 ccaggaaggt catcgccgcc ggaggggttt ggagcgacga catccaggca atggccggag
     4441 atcgcggtca ggacgtcagc gcctccaaag gggtccatct ggtggtcccc cgcgaccgga
     4501 tcaactccag caccgggatg attctgcgca ccgagatcag cgtgctgttc gtcattccct
     4561 ggggcaggca ctggctcatc ggaaccaccg acacaccgtg gtcgctgcgc cgcgcccatc
     4621 ccgccgcctg tctcgacgac attcagtatc tgctgcgatg ggtgaacacg gctctggccg
     4681 tgccgatcgg gcccgaggac gtggtcgggg tgtacgccgg cctcaggccg ttgttggccg
     4741 gggagtcgga cgagacgagc aagctgagcc gggagcacgc ggtgatcgtg tccgacaccg
     4801 gtctgatcac
//
LOCUS       DBNHXU010000237        11103 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2259132, whole genome shotgun sequence.
ACCESSION   DBNHXU010000237 DBNHXU010000000
VERSION     DBNHXU010000237.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 11103)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11103)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11103
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2259132"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..348
                     /gene="msrB"
                     /locus_tag="AC161X_05880"
     CDS             <1..348
                     /gene="msrB"
                     /locus_tag="AC161X_05880"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015247977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HIR4989115.1"
                     /translation="TERAFTGKYWNSKAHGTYRCAGCGEELFSSETKYDSGSGWPSFY
                     QPIQLEKVAEDRDSSYGMVRTEVRCSNCGGHLGHVFDDGPNPTGLRYCINSAALDLEE
                     EDRKSESGSGLGE"
     gene            complement(319..900)
                     /locus_tag="AC161X_05885"
     CDS             complement(319..900)
                     /locus_tag="AC161X_05885"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4989116.1"
                     /translation="MPDTSPSDLPTIAVTLRDGSIAELRPIHPDDRGLLAEGLSLMSI
                     ESRFARFGVGVDHLTPSELDYLTKVDQVGHVAWGAAIDNEPAGVGRYIRFSDPICADV
                     AVTVVDRFQGRGLGRVLFEALVASARANQLTELCFAVQAFNEPVRRILDDVDVDLTES
                     GGLVQGRIEVSSLPVTERDERFGELLAEARARL"
     gene            963..1889
                     /locus_tag="AC161X_05890"
     CDS             963..1889
                     /locus_tag="AC161X_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010865095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-methyltransferase"
                     /protein_id="HIR4989117.1"
                     /translation="MARQRLATVTSEFGSGKRESHDSSPFYERFSPPLISDDENVATP
                     VAIDHLFHGDAREMADDQVADRSVALVVTSPPYFAGKEYETDTTTGHVPASYIEYLEL
                     LEEVLAVCLRKLEPGGRMAVNVANLGRKPYRSLSSDVMAILQDRLGMLLRGEVIWQKA
                     KGAGGSCAWGSYRSPQNPVLRDVTERVVIASKGRFDRAIKRAKREALGLPNVATIETE
                     EFLEATLDVWEIPSERASRVGHPAPFPVALPRRLIELYTYTNDLVLDPFIGSGSTAIA
                     AVETARHYVGFDTDAAYLALANERIAASGGSP"
     gene            1886..2356
                     /locus_tag="AC161X_05895"
     CDS             1886..2356
                     /locus_tag="AC161X_05895"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008832177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight
                     protein-tyrosine-phosphatase"
                     /protein_id="HIR4989118.1"
                     /translation="MKILTVCLGNICRSPAAAAAIKEAAVDAGFEVEVDSAGTGSWHI
                     GQPPHPESVAAGARAGLRIDGRARRVTASDFDRFDVILAMDRSNLRELTGIAPSKASQ
                     AKLRLFRTYDPDSDEDEIPDPWGGPTEGYEETIRIVRAAARGLVEDLFVRAQAW"
     gene            complement(2344..3063)
                     /locus_tag="AC161X_05900"
     CDS             complement(2344..3063)
                     /locus_tag="AC161X_05900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989119.1"
                     /translation="MNLPALPSGFAETRNKLHQLAFFALAPARYKAVGRMGLRATPGG
                     FGTPEFDGRVARIEGDLIVIEDDGNVATQTISTIGSACDFLGIEYQEVWFPDFGDPLP
                     PVAPSSELSVDVEGALAIANWFQFGGDVLARLRGYGLQGDEVSETQIWPEHFDAAIEM
                     GNETLGQRASFGASPGDAAHPEPYVYVSAWGKIYRSNRYWNDEAFNGSSLGYDRLMED
                     ADPVTAALAFMLEGYRILHQA"
     gene            complement(3060..3572)
                     /locus_tag="AC161X_05905"
     CDS             complement(3060..3572)
                     /locus_tag="AC161X_05905"
                     /inference="COORDINATES: protein motif:HMM:NF019301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HIR4989120.1"
                     /translation="MNELDFCLTLLRVWAGIVLLAHGINHARTQEGTATWFAKKGFRS
                     PALNAKISAGNEILLGLGLIVGLLTSLAAAGLAATMFVAFWSIHRFAGFFNFRRPDEG
                     YEYVTTLAFVALVIAILGPGSVSLDSAIGISDDLDGWLGAGIVGIGLAVAGLQLAAFW
                     RKPNPEVSET"
     gene            3641..4066
                     /locus_tag="AC161X_05910"
     CDS             3641..4066
                     /locus_tag="AC161X_05910"
                     /inference="COORDINATES: protein motif:HMM:NF012264.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HIR4989121.1"
                     /translation="MKYWAEGALALAVTLVLGAGLWLFTGQPIGDETTATTAPVVVDT
                     EAAARGEVLASETGCLACHTIDGASGVGPTWKGVAGATRPLVSGEQVVADNAYLFNSI
                     VDPNSQVVSGFEPVMPTSFADQLTEDEINDLVEYIKSLS"
     gene            4237..6051
                     /gene="dnaK"
                     /locus_tag="AC161X_05915"
     CDS             4237..6051
                     /gene="dnaK"
                     /locus_tag="AC161X_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HIR4989122.1"
                     /translation="MARAVGIDLGTTNSVIAVLEGGEPTIIANAEGGRTTPSVVSFKD
                     GEVLVGEVAKRQSITNPDQTVRSVKRHMGTDWSIEVDGKKLTSQEISARILMKLKRDA
                     ESYLGDKVTDAVITVPAYFNDAQRQATTEAGQIAGLNVLRIINEPTAAALAYGLDKES
                     DQTILVFDLGGGTFDVSVLEIGDGVFEVKATSGDTHLGGDDWDQKVIDWLVEGFKNDH
                     GVDLAKDRMALQRLKEAAEKAKIELSTVVETEVNLPFITATSDGPLHLQRKLTRSEFQ
                     KLTEELLERTKRPFQQAVKDAGIAIDDIEHVVLVGGSTRMPAVQELVKSLTGKDPHRG
                     VNPDEVVAAGAAIQAGVLKGDVKDILLLDVTPLTLGIETKGSVMTRMIERNTTIPTKR
                     SEIFTTAENNQPEVEIHVLQGERTMANDNKSLGKFRLTGIPPAPMGVPQIEVSFDIDA
                     NGIVNVNAKDLATGKEQAMTITGGSALAEGEIDRMIKDAEAYAEEDAKRREAVETRNQ
                     ADQLVHQTDKLLEEQGEQMTEEEKTGVESALAELKATLGNEASDNSEVRSKMDSLISA
                     SQAMATRLYQQAAAAGEAEEAAPEDEDVVEAEIIEDDE"
     gene            6044..6598
                     /locus_tag="AC161X_05920"
     CDS             6044..6598
                     /locus_tag="AC161X_05920"
                     /inference="COORDINATES: protein motif:HMM:NF013212.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HIR4989123.1"
                     /translation="MSDLEPEVVIGEIVEASSTPGFDLPEDPEEAVKYLTTLLVESRD
                     EATSYLDDLRRVAADFDNFRKRALREQSTTLDRAAERVVRELLPVLDSFDGALAVEPA
                     TDGERRLLSGMIGTREQLLKALGQEGLEVIPTIGEPYDPEIHEPVGAPEGGGPFVVSQ
                     ELRRGYKLGGKVLRAALVGLESER"
     gene            6595..7710
                     /gene="dnaJ"
                     /locus_tag="AC161X_05925"
     CDS             6595..7710
                     /gene="dnaJ"
                     /locus_tag="AC161X_05925"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HIR4989124.1"
                     /translation="MNRDWVDKDFYEILGVGSEATPEEIKKSYRKLAQTYHPDANPGN
                     DEAEEKFKEISEAYATLSNAEQRKEYDQVRKLVASGGYSGYGQGAGGFGGQQVRVEDL
                     SDLLGGLGGLGDLFGFGSQSRSGPARGADVQADLSISFEDAAAGITTTIKVKGAAPCS
                     HCGGSGAEPGTQVTTCPTCGGRGVVAQNQGFFSFSQPCPQCRGSGRLIETPCSVCRGS
                     GAETRTRDVNVRVPAGVQDGNTLRIRGKGAPGRNSGPAGDLLVRVRVATSRTFGRKGN
                     DLTLSVPITYPEAVLGTKLEVPTLNGAVKLKIPAGTPSGKTFRVRGKGVIPERGRAGD
                     LLVTVQVAVPTKVGKEEKQLIEDLAKYETDDVRSDLR"
     gene            7721..8113
                     /locus_tag="AC161X_05930"
     CDS             7721..8113
                     /locus_tag="AC161X_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012224844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heat shock protein transcriptional repressor
                     HspR"
                     /protein_id="HIR4989125.1"
                     /translation="MNETTRAVFVISVAAELAGMHPQTLRIYERKGLIDPFRTPGGTR
                     RYSREDIERLQLIQELTSQGLNLEGVRRVLALQEENRVLKRKLDKLRDKMDDIEEEME
                     QRIEEVRRANRHELILRSQVRSLFPEDR"
     gene            8113..8385
                     /locus_tag="AC161X_05935"
     CDS             8113..8385
                     /locus_tag="AC161X_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989126.1"
                     /translation="MADTYQGPVRVLGGDGILLTTGLATLETDPDMGNWRGSLETLRG
                     TGVAGKALVVELEIPGGSTGRAQLTPVSESGDRATSSLVGFGSKPF"
     gene            8410..8985
                     /locus_tag="AC161X_05940"
     CDS             8410..8985
                     /locus_tag="AC161X_05940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989127.1"
                     /translation="MGAAADCESIGAGLLRQPVNSLTTLAFVLAGILVVTRRPERRWV
                     GIALVATGIGSFLFHGPMPGASEWAHDVSLAWLLLVVSADGSRWERWSRIPGLAGIAV
                     IFGLFPPVADPISVALTIVALISVLRTDRSAATWAAVSLLAVSGLLGRLGATNWPWCD
                     PDSALPLHGLWHLGAAAAVTIWALMTPPLNR"
     gene            complement(8975..>11103)
                     /gene="clpB"
                     /locus_tag="AC161X_05945"
     CDS             complement(8975..>11103)
                     /gene="clpB"
                     /locus_tag="AC161X_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012870927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA];
                     GO_process: GO:0042026 - protein refolding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HIR4989128.1"
                     /translation="VTSDDPESTFNALEQYGRDLTAEARKGGLDPVIGRDEEIRRVIQ
                     VLSRRTKNNPVLIGEPGVGKTAIVEGLARRVADGDVPEGLKTKRIISLDLASMVAGAK
                     YRGEFEERLKAVLEEIRAAGGEIITFLDELHTLVGAGAAEGAMDASNMLKPMLARGEL
                     RLIGATTLDEYRKHIEKDAALERRFQPVLVEAPSVEETVGILRGLKERYEVHHGVRIT
                     DSALVAAAVLSDRYIPARFLPDKAIDLIDESASRLRIEIDSMPEEIDDLTRRRIQLEI
                     EREALRKETDPAAADRLQSVEQEVADLTEEIDALTARWNLEKDVITAIQAIKEEMEET
                     RAAAERAEREGDLAKAAELRYGTLGALEAQLAEKEADLAHLHEQGRMLSEEVTEQDVA
                     EVVARWTGIPVSRLMEGETQKLIRLEEELHRRVVGQHHAVTAVANSVRRSRAGLADPN
                     RPSGSFLFLGPTGVGKTELARALADYLFDDERAMVRIDMSEYMEKHSVSRLIGAPPGY
                     VGYDEGGQLTEAVRRRPYAVLLLDEVEKAHPDVFNVLLQLLDDGRLTDGQGRTVDFTN
                     VVLIMTSNLGSENIQPDQPFEVVEERVLEAVRAHFRPEFLNRIDEIIVFDRLTREDLR
                     HIVEIQLESFHKRLAERRIELEVSEAALDLLAERGFDPVYGARPLKRTIQRELADPLA
                     LRLLSGEFSEGDTVKVTVEEGELTID"
BASE COUNT         2107 a         3571 c         3498 g         1927 t
ORIGIN      
        1 accgagcggg cgttcaccgg caagtactgg aactcaaagg cccacgggac ctaccgctgc
       61 gccggctgtg gtgaggagtt gttctcgagc gagaccaagt acgactcggg ctcgggttgg
      121 cccagcttct accaaccaat ccaattggag aaggtcgccg aggacaggga cagctcctac
      181 gggatggttc gcaccgaagt ccgctgctcc aactgtggcg gccacctcgg ccacgttttc
      241 gacgatggac caaaccccac cgggctccgc tactgcatca actcggcggc actggacctt
      301 gaagaggagg accggaagtc agagtctggc tctggcctcg gcgagtagct cgccaaacct
      361 ctcgtctcgc tcggtaacgg gcaaagacga cacctcgatc ctcccctgaa ccaggcctcc
      421 gctttcggtg aggtcgacat ccacgtcgtc gaggatccgt cgcaccggct cgttgaacgc
      481 ttggacggcg aaacacagct cggtcagctg attggcccgg gcggaggcga ccagggcttc
      541 aaagagcacc cggcccagac ctcgaccctg gaatctgtcg acaacagtca ccgcgacgtc
      601 ggcgcagatg gggtccgaga accggatgta acggccgaca ccggcgggct cgttgtcgat
      661 ggccgccccc cacgccacat gcccgacctg atcgaccttg gtcaggtagt ccagctcgga
      721 gggggtgagg tggtctaccc ccactccaaa tcgggcaaac cggctctcga tcgacatgag
      781 gctcaagcct tcggcgagca ggccccggtc atcgggatgg atcgggcgca gttcggcaat
      841 cgagccatcg cgaagggtga cggcaatcgt tggcagatcg gatggagatg tgtctggcat
      901 gaggtgcttg ctgcagcaag cataccgcga gcaggagggc tactcctgta acttcaccgt
      961 ggatggctag gcagaggctg gcaacagtca cctccgaatt tgggtcgggg aagagggagt
     1021 cgcatgactc ttcacccttt tatgagcggt tcagtccgcc tctgatctcc gacgatgaga
     1081 atgtcgcgac gccggtggcg atcgaccacc tgttccacgg cgatgcccgg gagatggcag
     1141 acgatcaggt ggcagatcgc tcggttgccc tggtcgtcac atcaccgccc tacttcgccg
     1201 gcaaagagta cgagacggac accacaaccg gccacgtgcc cgccagctat atcgagtatc
     1261 tcgaattgct ggaggaggtg ctggcggtct gtctgcgcaa gctcgagccc ggtggtcgga
     1321 tggcggtgaa tgtcgccaac ctgggacgca agccctaccg gtcgctgtcc tcggatgtca
     1381 tggccattct ccaggaccgg ctgggaatgc tgcttcgagg agaggtcatc tggcaaaagg
     1441 ccaagggagc cgggggcagc tgtgcctggg ggtcatatcg ctccccgcaa aaccccgtcc
     1501 tcagggacgt caccgagaga gtcgtgatcg catcgaaagg acgcttcgac cgggcgatca
     1561 agagggccaa gcgggaagcg ctcggcctgc ccaatgtcgc cactatcgag accgaggagt
     1621 tcctcgaggc aacactcgat gtgtgggaga tcccgtccga gcgggcgagc cgtgtgggcc
     1681 accctgcacc cttccccgtt gccctccctc gccgactgat cgagctctac acctacacca
     1741 atgacctggt tctcgatccg ttcatcgggt cggggagcac cgccatcgcc gcggtcgaga
     1801 cggcccgcca ctatgtgggc ttcgacaccg atgccgcata tctggccttg gccaacgagc
     1861 ggatcgctgc ctccggtggg agcccatgaa gatcctcacc gtgtgcctgg ggaacatctg
     1921 caggtccccg gccgcggccg cggctatcaa agaggcggcg gtggacgcag gcttcgaagt
     1981 cgaggtcgat tctgcgggca caggttcctg gcatatcggc cagccgccac accccgaatc
     2041 ggtggccgcc ggggcgagag ccggtcttcg gatcgacggg cgcgctcgtc gggtcaccgc
     2101 atctgacttc gaccgtttcg acgtgatcct cgccatggac cgctccaatc tgcgggagtt
     2161 gaccggaatc gcacccagca aggcgtccca ggccaaactg cgcctcttcc gcacctatga
     2221 cccggattcg gacgaagacg agattccgga cccctgggga ggacctacag agggctacga
     2281 ggagacgatc cggattgtgc gcgccgcggc gcggggtctc gtcgaagatc tctttgtccg
     2341 ggctcaggcc tggtgaagaa tgcggtagcc ctccagcatg aaagctaggg cggcggtcac
     2401 cggatccgca tcctccatga gtcggtcgta gccgaggctg gagccattga atgcctcgtc
     2461 gttccagtaa cgattggagc ggtagatctt gccccatgcc gagacataga cgtagggctc
     2521 aggatgcgcg gcatcgcccg gtgatgcccc gaaactcgcc ctctgcccca aagtctcatt
     2581 gcccatctcg atagcggcgt cgaaatgttc aggccagatc tgggtctccg agacctcgtc
     2641 gccttgcagt ccatagccgc ggagccttgc caggacgtcg ccgccgaact gaaaccagtt
     2701 ggcgatggca agcgcacctt cgacatcgac agatagctca gaggatgggg cgacaggcgg
     2761 aagcgggtct ccgaagtcgg gaaaccaaac ttcctggtat tcgatgccga gaaaatcgca
     2821 cgccgatcca atcgtggaga tggtttgggt cgcaacgttt ccgtcgtcct cgatgacgat
     2881 caggtcccct tcgatccggg cgacccggcc atcgaattcg ggggtcccga atccgccggg
     2941 cgtggcgcgc aggcccatcc gacccaccgc tttgtagcgg gcgggggcca gagcgaagaa
     3001 cgcgagctga tggagcttgt tccgcgtctc ggcgaagcct gaaggaagtg cgggcaggtt
     3061 caagtctccg aaacctccgg gttgggcttc cgccagaaag cagcgagctg cagaccggcg
     3121 acggccaggc cgatccccac gatcccggct ccaagccacc catcgaggtc atcggagatg
     3181 ccgatggccg agtcgagcga taccgaaccg gggccgagaa tggcgatgac cagtgccacg
     3241 aaggccaggg tggtcacgta ctcgtagccc tcgtccggac gacgaaagtt gaagaaaccg
     3301 gcaaagcgat gaatcgacca gaaggccacg aacatcgtcg ccgccagccc ggcagcggcc
     3361 agtgaggtca gcaggccgac gatcagcccc agcccgagga ggatctcatt gccggcggag
     3421 atctttgcgt tgagggccgg cgaccgaaag cccttcttgg cgaaccacgt ggccgtgccc
     3481 tcctgggttc gggcgtggtt gatgccatgg gcgaggagga cgatccctgc ccacacgcgg
     3541 agcagggtca gacagaagtc gagctcgttc atgggtcaga accctaggtt ccggccgacg
     3601 attgttacgg ttccgatgac ctgatggtgg aaaggcatca atgaagtact gggcagaagg
     3661 agcgctcgcg ctagccgtca ctctcgtgct gggcgccggt ctttggttgt tcaccggaca
     3721 gccaattgga gacgagacca ccgccacgac agcaccagtg gtggtagaca ccgaggccgc
     3781 ggcgcggggt gaggtccttg cctccgagac tggttgcctt gcctgtcaca ccatcgacgg
     3841 ggcatccggt gtcgggccaa cctggaaggg cgtggcaggc gccacccggc cgctggtctc
     3901 aggcgagcaa gtggttgccg acaacgccta tctgttcaat tcgatagtcg acccgaattc
     3961 acaggtggtg agcggtttcg agccagtgat gcccaccagc ttcgccgacc agctcaccga
     4021 agacgagatc aacgacctcg tggagtacat caagtctttg tcataatgcc ggctgacttc
     4081 gcctctccgc gtgtcgagta acccccctcc ccccttcggg ggactccccc caccgctcgc
     4141 tgcgctcact ggggggagaa acacggaata tgacaatacc tcactcaggt tgtacccttc
     4201 gtacacactc aacctgaacc ggaggttcat caacaaatgg cacgcgcagt aggtatcgat
     4261 ctcggcacca ccaacagcgt catcgccgtg ctcgaagggg gcgagcccac catcatcgcc
     4321 aacgccgagg gcggccgcac caccccttcc gtagtctcgt tcaaggacgg cgaggttttg
     4381 gtgggtgagg tagccaaacg gcaatcgatc accaaccccg accagaccgt tcgctcggtc
     4441 aagcgccaca tggggaccga ctggtcgatc gaagtggatg gcaagaagct cacctcccaa
     4501 gagatctcgg cccgcatcct catgaagctg aagcgggacg ccgagtccta tctcggcgac
     4561 aaggtcaccg atgcggtgat caccgttccc gcctacttca acgacgccca gaggcaagcg
     4621 accacagaag ccggacagat tgccgggctg aatgtgctcc gcatcatcaa cgagccgaca
     4681 gcggccgccc tcgcctacgg gttggacaag gagagcgacc agacgatcct cgtcttcgac
     4741 ctgggtggcg gcaccttcga cgtctcggtc ctcgagatcg gtgacggcgt cttcgaggtc
     4801 aaggcgacct caggcgacac ccacctcggt ggcgacgact gggaccagaa ggtcatcgac
     4861 tggctggtcg agggtttcaa gaacgaccat ggcgtcgacc tggccaagga ccgcatggcc
     4921 ctgcaacgtc tcaaggaagc ggcggagaag gcgaagatcg agttgtcgac tgtcgtcgag
     4981 accgaggtca acctcccctt catcaccgcc acctccgacg gcccactgca tctccagcgc
     5041 aagctgaccc ggtccgagtt ccaaaagctc acggaggagc tcctggagcg aaccaagcgg
     5101 cccttccagc aagccgtcaa ggatgccgga atcgcgatcg acgacatcga gcatgtcgtc
     5161 ctcgtcgggg gatcgacccg gatgcctgca gtccaggaac tggtcaagag cctcaccggc
     5221 aaggaccctc accgtggtgt gaaccccgat gaggtggtgg ccgcgggcgc tgccatccag
     5281 gccggagtgc tcaagggtga tgtgaaggac atccttctcc tcgacgtaac cccgctgacc
     5341 cttggcatcg agaccaaggg ctcggtgatg acccggatga tcgagcgcaa caccacgatc
     5401 cccaccaagc ggtcggagat cttcaccacg gctgagaaca accagcccga ggtcgagatc
     5461 cacgtcctcc agggcgagcg caccatggcc aacgacaaca agagcctggg caagttccgc
     5521 ctcaccggga tcccaccggc tccgatgggc gtcccccaga tcgaggtttc cttcgacatc
     5581 gacgccaacg ggatcgtcaa tgtgaacgcc aaggatctcg ccactggcaa ggagcaggcg
     5641 atgacgatca ccggcggctc agctctcgcc gaaggtgaga tcgaccggat gatcaaggat
     5701 gccgaggcct atgccgagga agacgccaag cgccgcgagg cggtggagac ccgcaatcag
     5761 gcggaccagt tggtccatca gaccgacaaa ctcctcgagg agcagggcga gcagatgacc
     5821 gaggaagaga agaccggtgt cgagtcggcg ctggccgaac tcaaggcgac gctcggcaac
     5881 gaagcgagcg acaactcgga agttcgatcc aagatggaca gcctgatctc ggcgagccag
     5941 gcgatggcca cccgtctcta tcagcaagcg gcagccgcag gagaggccga agaggccgct
     6001 cccgaggatg aggacgtcgt cgaagccgag atcatcgagg acgatgagtg atttagagcc
     6061 ggaagtcgtc attggggaga tcgtcgaggc gtcgagcacg cccgggttcg atctccccga
     6121 agacccggag gaggccgtca agtacctgac aacccttctc gtcgagtcgc gggacgaggc
     6181 aacctcatat ctcgacgatc tgcgccgtgt cgctgccgac ttcgacaact tccgcaagcg
     6241 cgccctgcgg gaacagtcga caacgctcga ccgtgccgcc gagcgcgtcg ttcgcgagct
     6301 gttgccggtt ctcgattcgt ttgacggggc actcgcggtc gagccagcca ctgatggtga
     6361 gaggcgcctt ctttcaggaa tgatcgggac cagggaacaa ctgctcaaag ccctgggaca
     6421 ggagggtcta gaggtcatcc ccacgatcgg tgagccctat gaccctgaga tccatgagcc
     6481 ggtgggagca cccgagggtg gtggcccatt cgtcgtctcc caggagctgc gccggggcta
     6541 caaactcggc gggaaggtcc tgcgcgccgc cctggtgggc ttggaatccg agcgatgaac
     6601 cgggattggg tcgacaagga cttctacgag atccttggcg ttgggtccga ggccacgccc
     6661 gaagagatca agaagtccta ccgcaagctg gctcagacct atcacccgga cgccaacccc
     6721 gggaacgatg aggccgagga gaaattcaaa gagatctccg aggcttatgc cactctgtca
     6781 aatgccgagc aacgcaagga gtacgaccag gtccgaaagt tggtcgcctc gggcggctac
     6841 tccggatacg gccaaggggc cggtggcttc ggcggacagc aggtgcgggt cgaagacctc
     6901 tccgacctcc ttggagggct gggcggtctc ggcgacctct tcgggttcgg ctcccaatcg
     6961 aggagcggtc ccgccagggg cgccgatgtc caggccgatt tgagcatcag cttcgaggat
     7021 gcagcggccg gaatcactac gacgatcaag gtgaagggcg ccgccccctg ttcacactgt
     7081 ggggggtcgg gcgcagagcc gggcacccag gtcaccacct gcccgacctg cggaggccgc
     7141 ggtgtcgtcg cccagaatca gggattcttc tccttctccc agccgtgccc gcagtgccgg
     7201 ggatcgggcc gtctcatcga gactccgtgc tctgtctgtc gcggctcagg agctgagacc
     7261 aggacccgtg atgtgaatgt gcgggtcccg gccggagtcc aggacggcaa caccctccgg
     7321 atcaggggca agggtgcacc gggccgcaac agtggccccg cgggcgacct tctcgtgcgg
     7381 gttcgggttg cgaccagccg gaccttcggc cgcaagggaa acgacctcac cctctcggtc
     7441 ccgatcacct accccgaagc tgtgctgggg accaagctcg aggtgccaac cctcaatggg
     7501 gcggtcaagc tcaagattcc ggccggcacc ccttccggaa agaccttccg ggtgagaggc
     7561 aaaggcgtca ttcccgagcg gggacgggcc ggcgatctac tggtcaccgt ccaggtcgcg
     7621 gtgcccacca aggtcggcaa ggaagagaag caattgatcg aagacctcgc caagtatgag
     7681 accgatgacg tgcggtccga tctgagatga tggtgactcg atgaacgaga ccacccgcgc
     7741 ggtgttcgtg atctcggtgg cggctgagct cgccgggatg cacccgcaga ccctccgcat
     7801 ctacgagcgc aaaggcctca tcgatccttt tcgcactcct gggggcaccc gccgttactc
     7861 acgcgaggac atcgagcgtc ttcagctgat tcaggagctc acgagccagg gcctcaacct
     7921 cgaaggggta cgccgggttc tggcacttca ggaggaaaac cgcgtcctga agcggaagct
     7981 ggacaaactg cgggacaaga tggacgacat cgaggaagag atggagcagc ggatcgagga
     8041 ggtccgtcgg gccaaccgcc acgaactcat cttgagaagc caggtccggt cgttgtttcc
     8101 cgaggatcgc tgatggccga cacctatcaa ggcccggtcc gggtcctcgg tggtgacggg
     8161 atcctgctga ccaccggcct ggccacgctc gagaccgatc cggacatggg caattggcgg
     8221 ggctcgctcg agacgttgcg gggaacgggg gtagccggga aggcgctcgt cgtcgagctg
     8281 gagatccctg gcggatcgac gggtcgagcc caattgaccc cggtcagcga gtccggcgac
     8341 cgggcaacat catcgctcgt cggtttcggc tcaaagcctt tctgaacgaa ccgtcgttac
     8401 cgtgccctaa tgggtgcagc cgccgactgc gagtcgatcg gagctgggct ccttcgacag
     8461 ccggtcaact ccctgaccac tctcgcattc gtcctcgccg gaatcctcgt tgttaccagg
     8521 cgaccggagc ggcgctgggt cggaatcgcc ttggtcgcca ccggaatcgg atcattcctc
     8581 tttcacggtc cgatgccggg tgccagcgaa tgggcccacg acgtcagcct cgcctggctt
     8641 ttgttggtgg tctcagccga cgggtctcga tgggagcgtt ggtcccggat tcccggtctg
     8701 gctggcatcg cggtgatatt tgggctgttt cccccggtgg ccgacccgat ctcggtagct
     8761 ctgaccatcg tcgccttgat ctcggtgctg agaacggatc gatcggccgc aacctgggcg
     8821 gcagtatcac tactcgcggt cagcgggctc ctgggtcgtc tgggtgccac caactggccc
     8881 tggtgcgacc ccgactcggc cctgccactg catgggctct ggcatttggg ggctgcggcc
     8941 gcagtgacca tctgggccct catgaccccg ccgctcaatc gatagtgagc tctccctcct
     9001 cgaccgtgac cttgacggtg tccccttcac tgaattcgcc cgagagcagc cgcagggcga
     9061 gcgggtcggc caactcccgt tgaattgtcc gcttcaaggg ccgggccccg tagacggggt
     9121 cgaatcctcg ctcagcgagt aggtcaagcg cagcttcgga tacttccaac tcgatccgtc
     9181 gctcagcgag ccgtttgtga aacgactcca gttggatctc aacgatgtgt cgcaggtctt
     9241 cccgggtgag cctgtcgaag acgatgattt cgtcgatgcg gttgaggaac tccggccgaa
     9301 agtgggcccg caccgcctcc agcaccctct cctccactac ctcgaacggc tgatccggtt
     9361 ggatgttctc cgacccgagg ttcgaggtca tgatgaggac cacgttggtg aagtccacag
     9421 tcctcccctg accgtcggta agcctcccgt catcgagaag ctgcagcagc acgttgaaaa
     9481 catcgggatg cgccttctcc acctcatcca ggaggagcac ggcgtagggg cggcgtcgca
     9541 ccgcttctgt cagctgaccg ccctcgtcgt agccgacata cccgggaggg gcgccgatga
     9601 gccggctcac cgagtgcttc tccatgtact ccgacatgtc gatccggacc atcgccctct
     9661 cgtcgtcgaa gaggtagtcg gcaagagccc gcgccagttc ggtcttcccg acaccggtgg
     9721 gaccgagaaa caggaacgac cccgaaggcc ggttggggtc ggcgagcccg gcacggctgc
     9781 ggcgaaccga gttggcgacc gcggtcaccg catgatgctg gccgacgacc cgccggtgca
     9841 gctcctcttc gagcctgatc aacttctggg tctcgccctc catcagcctc gaaaccggga
     9901 tcccggtcca cctggctacg acctccgcaa cgtcttgctc ggtcacttcc tcgctgagca
     9961 tccgaccctg ttcgtgaagg tgcgccaggt cggcctcctt ctcagcaagc tgagcctcga
    10021 gagcccccag ggttccgtag cgaagctccg ccgccttggc gagatcccct tctcgctccg
    10081 ctctctcggc agcggcgcgg gtctcctcca tctcctcctt gatcgcctgg atggcggtga
    10141 tcacgtcctt ctccaggttc cagcgcgcag tgagggcatc aatctcctcg gtcaggtcgg
    10201 cgacctcctg ctccacactc tggagccgat ctgcggcggc tggatcggtc tccttcctca
    10261 gcgcctcgcg ttcgatctcc aactggatcc ggcgccgggt caaatcatcg atctcctcgg
    10321 gcatcgaatc gatctcgatc cggagccgac ttgcagactc gtcgatcagg tcgattgcct
    10381 tgtcgggtaa gaatcgggcc gggatgtacc ggtcggacaa cacggctgcc gccaccaggg
    10441 cggagtcggt gatccgaacc ccgtggtgga cttcgtagcg ctccttgagc cccctgagaa
    10501 tccccacggt ctcctccaca gatggcgcct ccaccagcac cggctggaag cgccgctcca
    10561 gggctgcatc cttctcgatg tgcttgcggt actcgtcgag tgtggttgcg ccgatgagtc
    10621 gcagctcgcc acgggcgagc atcggcttca gcatgttgga tgcatccatc gccccttcgg
    10681 cagcgccggc gccgaccagg gtgtggagct cgtccaggaa agtgatgatc tcgccaccgg
    10741 cggcccggat ctcctcgagc accgccttga gccgctcctc gaactccccg cggtacttgg
    10801 cgccggccac catcgaggcc aggtcgagcg agatgatgcg cttcgtcttc aggccttcgg
    10861 gcacgtctcc gtcggccaca cgccgggcga gcccttcgac gatggccgtc ttgcccaccc
    10921 ccggctcgcc gatgagcacc gggttgttct tggtccgtcg ggaaaggacc tgaatcaccc
    10981 tccggatctc ctcgtctctc cctattaccg ggtcgaggcc tcccttgcgg gcttctgctg
    11041 tgagatcccg gccgtactgc tcgagggcat tgaaggtcga ctccgggtcg tcactggtca
    11101 ccc
//
LOCUS       DBNHXU010000238         1519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2854228, whole genome shotgun sequence.
ACCESSION   DBNHXU010000238 DBNHXU010000000
VERSION     DBNHXU010000238.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2854228"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            84..1256
                     /locus_tag="AC161X_05950"
     CDS             84..1256
                     /locus_tag="AC161X_05950"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014277471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-[acyl-carrier-protein] synthase
                     family protein"
                     /protein_id="HIR4989129.1"
                     /translation="MSRRRVVVTGTGAVAAPGTGTDDLWKGLQLAPDGPGPYLVKDWD
                     PEPWIPLREHRRLDRFTQFAMMAAHEAFEHSGPLEVEPNRVAVSLATGIGGLESLEIL
                     VHSSDADDPRASPFLIPMMMANAAAAAISIKYGFGGQVTTPVVACAAGAQSILDGLRQ
                     IQWGYADAAVAGGTEAAARKSAAAGFSAARALSPSGTARPFDVDRDGFVMGEGAAILI
                     LEAEEVAKDRGANILAEVLGGASTADAHHITAPHPGGDGAERAIRLALADAGLELGDV
                     KYINAHGTGTDLNDKTEGLVIDRIFGEAQPAVSSIKGTTGHGLGASGSIEAVAVVEAI
                     RRRELPPNIGLINQDPEIHLFDIVREPREWEPGPVLSNSFGFGGHNTVLAFGPYLG"
BASE COUNT          289 a          483 c          477 g          270 t
ORIGIN      
        1 cgactacgac cgatggctgg aaggaatgcg cagactgcaa cgttctgcgt ttccaactcc
       61 gcgaatcggg cagaattgag cacatgagca gaaggcgagt ggtggtgacc ggcactggag
      121 ccgtcgctgc cccgggaacc ggcaccgatg acctctggaa aggtctgcaa ctcgcgcctg
      181 acgggcctgg tccctatctg gtcaaagatt gggacccgga gccgtggatc ccgctgcgcg
      241 agcaccgtcg cctggatcgg ttcacccagt ttgcgatgat ggccgcgcac gaggctttcg
      301 agcattcagg cccgctcgag gttgagccca accgggtcgc agtctcgctg gccaccggga
      361 tcggcgggtt ggagtccctc gagatattgg ttcacagctc ggacgccgac gatccacggg
      421 cctcgccatt cctaattccg atgatgatgg ccaacgccgc cgcggcggca atctcgatca
      481 agtacggctt cggtggccag gtcaccacac ccgtggtggc gtgcgcggcg ggagcccaat
      541 cgattctcga cggccttcgt cagatccagt ggggctatgc cgacgccgcc gttgccggtg
      601 gaaccgaggc ggcggcccgc aagtcggcgg cagcaggatt cagtgcggcc cgcgcgcttt
      661 cgccatcggg gacggcccgg ccattcgatg tcgaccggga tggcttcgtg atgggcgaag
      721 gcgccgcgat cctcatcctc gaagccgagg aagtcgccaa ggatcgcggg gccaacatcc
      781 tcgctgaggt tcttggtgga gcatccacgg cggacgccca ccacatcact gcgccccatc
      841 ctggcggcga tggtgcagag cgagcgatcc gcctcgcgct cgccgacgct ggtctcgaac
      901 tcggcgacgt caagtacatc aacgcccacg gcaccggaac cgacctcaac gacaagaccg
      961 aaggtctggt gatcgatagg atctttggcg aggcgcaacc ggcggtttcc tcgatcaaag
     1021 gcaccacagg ccacgggctt ggtgcatcag gatcgatcga ggcggtggcg gtcgtcgagg
     1081 cgatccggcg ccgcgagctt ccgccgaaca tcggcctcat caaccaggac cccgaaatcc
     1141 acctgttcga catcgtgcgt gagccacggg aatgggagcc gggcccagtc ctctccaatt
     1201 cgttcgggtt cggtggccac aacacagtgc tggccttcgg tccctatctc ggctgaccat
     1261 ttctcccccc aacgctcgct tcattcccaa ggtgagaaag cagggtcttt cggacgtatg
     1321 gcagccttcc cagggggtat acaatgggag ctgagagtcg ccatgcgaca cacgcctgta
     1381 acgcggagtt cacgagaact cgcatctccg ctgattgagt ggctactacg ccatcaacgg
     1441 aacggcgctc cggcaaacgt tcctgaagcg gaggctttat gacacttaaa acagcacatc
     1501 ccagtattgg ttttgtcag
//
LOCUS       DBNHXU010000239         4882 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2854552, whole genome shotgun sequence.
ACCESSION   DBNHXU010000239 DBNHXU010000000
VERSION     DBNHXU010000239.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4882)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4882)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4882
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2854552"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1669)
                     /locus_tag="AC161X_05955"
     CDS             complement(<1..1669)
                     /locus_tag="AC161X_05955"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR4989130.1"
                     /translation="MLSHIRSRRGMLLIATSVSGVLALFLDAPWSVVFGLLALGGFAG
                     LILILSVRLSQIVTRLGVLGRSIGRRSPTMAAPAERSTHETRAATRMTSAIDTLGSAA
                     DRLAAEPSMSERNLTPKQGHTPRVTIVVPCYNEERFVAEAIRSIAEQTFTDWECLVVD
                     DASTDSSLKEIWAATNSDQRFRVLRHQVNSGLSAARNTGLRAARGQLVTFLDADDLLM
                     RDSLMDRVIALANAMADPHVIGSYCGVTVAFEEVDLDELPDRMEGDSRGIIDLVSADA
                     ECPFNAHAPLLITERLRALGGFNERMRYGAEDWHLWYRAMRNGYVFVPSHYKTAVYRQ
                     KRNSMVRSMPDGHVLEANRLINAAYQHVDPELLVDPSPTPLTKPINEYQAQIHRARRA
                     IRFAAMALVAGDEAAARRALVVFQEAPLSLIERHIDLTDMTDRGMQRALAVSRDELTA
                     MSTLSEPIRSRMRALIGEVSKSYVPEPVTSVTKPSIGALLVPQHAGQIPQMLEAAAQS
                     GFSEGTIGVLTIEREGGSQGATIGAPESLPTWSLNEWALRTVTCSTAF"
     gene            1859..2695
                     /locus_tag="AC161X_05960"
     CDS             1859..2695
                     /locus_tag="AC161X_05960"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019846.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4989131.1"
                     /translation="MVKKPGEVGKDFDDYARKWAADSYKMEKAWDGSGVAHSDEALAY
                     PGDEWGGIDDLIALYANLFPKILPGSDKVSVLEIGAGGGRSTRAVVEVLGDRIDEYHV
                     VDVSQIFVNVLRERIEQPIEVHLVDDVDLGGLPKNGFQLCLAQSSWSHINMYDQYRYL
                     RDLRAVLTFGAPLVVNGQFLLGLGNDWAWNRFRRRVYQAEHHVEGVFHEMTGVGVIVE
                     QLVRLEYDIELIHDAGFIARRRQANPSSHLASLTAQIEFPYLPGLPQYAAGQTPRKVT
                     IG"
     gene            complement(2715..4808)
                     /locus_tag="AC161X_05965"
     CDS             complement(2715..4808)
                     /locus_tag="AC161X_05965"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyltransferase family protein"
                     /protein_id="HIR4989132.1"
                     /translation="MTARSLEYRPHLDGLRALAVLLVFVFHAARPGLPGGFIGVDVFF
                     VLSGYLITRILLIQYEKHGRIRIADFYARRIRRLMPAVILVVIVVVVHEALWGSILES
                     AARLRDAIGTLFYVENWNLIYQADQYFAESLSPSPLRHAWSLSIEEQFYLVWPLALIA
                     ILALTRRRMGRSMLVVLAAAAASAVAMALLFEPQLVTRAYYGTDARVQQPLIGAALAF
                     LVVRSNSTWKTPSKPWVWPVTASAMLGLLLIMARLFTGDSSFYYRGGSTLVAVTTAAL
                     VYSLERSPSGWAATGLGWRPLQQLGRISYGFYLWHWPIILWFAVPAGFSFWERRAVNL
                     AQFGLTVALSMASFIILESPIRDQTIRLGKLSSRSTIAIGMGTLALVGVFSISALQPD
                     DSTALVAGPDGPDTGSVSGGDSSATGDFAALAEQAAADRSYEPCPDDPQPCVKVEGRT
                     SDSPTVVLIGDSTAQAYDPALKDLATRYGFRYVQAAVGGCPISHRLIATGLDGELHKP
                     SNFMCYDQMPGIYDQVLADYDPVLFIATSWNETNQHVENGLLLEKGTPEHLASTDAAL
                     NATVDRLTAQGAQVAFIDVLPPGMSVDCLQDGGPDSAGCTRPVTEASGEAPYNAIFHK
                     IAEQRSDVVAITLTDIVCPDSECPLLIDGEVMRYDGGHFTGATSKRLAPTLAERLLAA
                     GIDLTAMGGSTSASG"
BASE COUNT          988 a         1411 c         1611 g          872 t
ORIGIN      
        1 cgaacgccgt cgaacaggtg accgtgcgca atgcccattc gttgagtgac caggtgggaa
       61 gggactcggg cgccccgatg gttgcgccct ggcttccacc ttcgcgctcg atggtcagaa
      121 ccccaatagt gccttcggag aaacctgatt gtgccgccgc ctcgagcatc tgagggatct
      181 gaccggcgtg ctggggcacc aagagcgccc caatcgaagg cttggtgacc gacgtgactg
      241 gctcaggcac atatgacttc gagacctcgc caatcagggc gcgcatgcgg ctgcggatcg
      301 gctccgagag ggtgctcatg gccgtcaatt cgtcgcgact caccgcgagc gcgcgttgca
      361 tgccccgatc cgtcatgtcg gtgaggtcga tgtgacgctc gatcagggag agcggagctt
      421 cctggaacac gaccaatgcg cgtctggcgg cggcctcgtc accagccacc aaggccatgg
      481 cagcgaacct gatcgctcgc cgcgcccgat ggatctgcgc ctggtattca ttgatgggct
      541 tggtgagggg ggtgggcgat gggtcaacga ggagctcagg gtccacgtgc tggtaggcgg
      601 cgttgatcag ccggttggcc tcgagaacat gcccgtcggg catggagcgg accatcgagt
      661 ttcgcttctg gcggtagacg gcggtcttgt aatgcgaggg aacaaagacg tacccgttgc
      721 gcatcgcccg ataccagagg tgccagtcct ccgctccgta gcgcatccgc tcgttgaagc
      781 ctccaagtgc tcgcagacgc tcggtgatca agagaggggc gtgggcattg aacgggcact
      841 cggcgtccgc tgataccaga tcgatgatcc cgcgggagtc accctccatc cgatccggaa
      901 gctcatcgag gtcgacctct tcaaaggcga cggtcacccc acagtaagac ccgatgacat
      961 gagggtccgc catcgcattg gcgagggcga tgacacggtc catgaggctg tcccgcatca
     1021 ggaggtcgtc ggcgtcgagg aacgtgacca actggcctcg ggcggcgcgc aacccggtgt
     1081 tgcgggcagc gcttagaccc gagttgacct gatggcggag aacccggaaa cgctgatccg
     1141 agttggtcgc agcccagatc tctttgaggg atgagtcggt ggaagcgtcg tcgaccacca
     1201 ggcactccca gtcagtgaag gtctgctcgg ctatcgaacg tatggcttcg gcaacgaatc
     1261 gctcctcgtt gtaacagggg acgacgatcg tcactcgtgg ggtatggccc tgcttagggg
     1321 tgagatttcg ctcgctcatc gaaggttccg cggcgagacg gtccgcggcg gatccgagag
     1381 tgtcgatagc gcttgtcatg cgtgtcgccg cacgagtctc atgtgtcgag cgctcggccg
     1441 gggcggccat ggtgggggag cgccgcccga tcgatctccc aaggaccccg agccgggtga
     1501 cgatctgact gagtcggaca ctcaggatca ggatcaagcc ggcgaatcct ccaagggcga
     1561 ggagcccaaa gacgactgac cacggtgcgt cgaggaatag ggcgagcacg ccggaaactg
     1621 aggttgcgat caggagcatc ccgcgacggg accggatgtg ggagagcatc gtcttcaatt
     1681 agtcatcccg ggtggctagg cgacgagtcc ggatcggccc ggacgcctcg gaaaccctag
     1741 ccggggatgg gtgattcacc agccaacgaa ggcgctcggg aggtgggtgc gtaggatcga
     1801 aatctgctct cgggccgacg acctttcgca acgatcccgc ccgagaagga gtgcacaagt
     1861 ggtcaagaaa ccaggcgagg tgggcaagga cttcgacgac tatgcccgaa agtgggccgc
     1921 agattcctac aagatggaaa aggcctggga tggatcaggc gtggcccact ctgacgaagc
     1981 tctggcttac cctggcgacg agtggggtgg catcgatgac ctgatagccc tctacgcaaa
     2041 cctgttccca aagatcctcc ccggttcgga caaggtgagc gtcctcgaga tcggtgccgg
     2101 cggtgggcgg tccaccaggg cagtggtcga ggtgctcggc gaccggatag acgagtacca
     2161 cgtagtggat gtatctcaga tattcgtaaa tgtcctcagg gagcggatcg agcagccgat
     2221 cgaggtccac ctggtcgacg acgttgacct tgggggtctt ccaaaaaacg gctttcaact
     2281 gtgcctggcc cagtcctcgt ggtcacacat caacatgtac gaccagtacc gctatctgag
     2341 agacctgagg gcggtcctga ctttcggagc tccattagtg gtgaatgggc agtttctgct
     2401 cgggctggga aacgactggg cgtggaacag gttccggcgc agggtctacc aagccgagca
     2461 ccatgtggag ggtgtcttcc atgagatgac cggggttggg gtcatcgtcg agcaactcgt
     2521 tcggctcgag tacgacatcg aattgataca cgatgccgga ttcattgctc gaaggaggca
     2581 ggcaaacccc tcgtcgcatc tagcaagtct taccgcacag atcgagtttc cctacctgcc
     2641 cgggcttccc caatacgccg ccggtcagac gccacgaaag gtgaccatcg gttgaaactg
     2701 tgatctccgc aggctcaacc tgaggccgag gtgctacctc ccatggccgt caggtcaatc
     2761 cctgccgcta aaagcctttc ggcgagagtc ggtgcaagcc tcttgctcgt ggcacccgtg
     2821 aagtggccac cgtcgtaacg catcacttca ccatcgatca ggagcgggca ctcgctgtcc
     2881 ggacaaacga tgtcggtcaa cgtgatagcc actacatcgc tgcgctgttc cgcgatcttg
     2941 tggaaaatcg cgttgtaggg cgcttcgcca ctggcctctg tgaccggtcg ggtgcatcca
     3001 gctgagtcag ggccaccatc ctggagacag tcaacgctca tccccggggg aagaacatcg
     3061 atgaatgcga cctgcgctcc ctgggctgtc agccggtcga ctgtggcgtt gagggcagca
     3121 tccgtcgacg cgagatgctc gggtgtgccc ttctcgagga gaagcccatt ctcgacgtgc
     3181 tggttggtct cgttccatga cgtggcgatg aacagaaccg ggtcatagtc ggcaaggact
     3241 tggtcgtaga tcccgggcat ttggtcgtaa cacatgaagt tggatggctt gtggagctca
     3301 ccatccaggc cggtggcgat caatcgatgg ctgatcgggc agccgccaac ggctgcctgc
     3361 acgtatcgga acccatagcg cgttgccaga tccttgagcg cagggtcgta ggcctgtgcg
     3421 gtcgagtcgc cgatcaggac gaccgtcggc gaatctgagg tccggccttc cactttgaca
     3481 cagggctgcg ggtcgtcggg acaaggttca taggaacgat cggccgcggc ttgctccgct
     3541 aaggcggcaa aatcgcccgt tgccgatgaa tctccgcccg acacagatcc ggtgtcaggg
     3601 ccgtccggcc cggcgaccaa cgctgtggag tcatcgggct ggagggcaga gatcgagaac
     3661 accccaacca gcgcaagggt gcccatgcca attgcgatgg tgctgcgtga gctgagcttt
     3721 cccaggcgaa tcgtctgatc tctgatgggg ctttcaagga tgatgaagct ggccatggat
     3781 aaggcaaccg tgagtccgaa ctgcgccagg ttcaccgccc gccgctccca gaaactgaag
     3841 ccggcaggca ccgcgaacca gaggatgatt ggccagtgcc agaggtagaa gccgtacgag
     3901 atccgtccga gttgctgcag gggtcgccac ccgaggccgg tcgccgccca gccgctagga
     3961 ctcctctcga ggctgtagac caatgcagcg gtcgtcactg caaccaaggt gctgcctcct
     4021 cggtaataga agctcgagtc gccggtgaac agacgcgcca tgatgagcaa cagccccagc
     4081 atggcgctcg ctgtgaccgg ccaaacccag ggtttggacg gggtcttcca cgtactgttg
     4141 gatcgcacca cgagaaaggc gagggcagca ccgatgaggg gctgctgtac gcgggcgtcg
     4201 gttccgtagt aggcgcgagt gacaagctgc ggctcgaaca acagggccat cgcgacggct
     4261 gatgcggcag ccgccgccaa cacgaccagc atcgatcgac ccatgcgcct gcgcgtgaga
     4321 gcgaggattg cgatcagggc taggggccag accaggtaga actgctcctc gatactgagc
     4381 gaccatgcgt ggcgcagggg agaagggctc aacgactcag cgaagtactg gtcggcctga
     4441 tagatcagat tccaattctc tacgtagaac agggtgccga tggcgtcgcg caatcgagcg
     4501 gctgactcga ggatgcttcc ccagagcgct tcgtggacca cgacgacgat cacaacgaga
     4561 atgacggccg gcatcaggcg gcggatccgt ctcgcgtaaa agtcggcgat gcgaatacgg
     4621 ccatgctttt cgtactggat cagcaggatc cgtgtgatga gatagcccga gagcacaaag
     4681 aagacgtcga ctccgatgaa ccctcccggc aagccggggc gggcagcgtg gaacacaaat
     4741 acgagcagga ccgccagagc gcggagcccg tcgaggtggg gccggtattc aagggaacgg
     4801 gcggtcatat ttcgaagcga gtcatggcgt gacggatcgg ggtaccgggt tgggaagggt
     4861 cagtcgaggt gccggggtcg gc
//
LOCUS       DBNHXU010000240         6077 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5434996, whole genome shotgun sequence.
ACCESSION   DBNHXU010000240 DBNHXU010000000
VERSION     DBNHXU010000240.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6077)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6077)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6077
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5434996"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC161X_05970"
     CDS             <1..279
                     /locus_tag="AC161X_05970"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4989133.1"
                     /translation="AIEHGNENRAEIPVEIEVRATAASVSVRVSDQGGDKVIPEAETP
                     DIEAKLAGEQTPRGWGLFLIGQMVDEIKTESNDNIHTVELVMNREGVS"
     gene            279..620
                     /locus_tag="AC161X_05975"
     CDS             279..620
                     /locus_tag="AC161X_05975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007919538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR4989134.1"
                     /translation="MDDGFGARVSWGDDGGVVHLTGTVNRLAKDGLESAYEEATSKPG
                     RVLIDFGEVDYINSTGIAVIVGVLARARAEGREVGAFGLSDHYREVFQITRLADFMAI
                     YEDEDAASAAR"
     gene            633..983
                     /locus_tag="AC161X_05980"
     CDS             633..983
                     /locus_tag="AC161X_05980"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR4989135.1"
                     /translation="MPTSNLTMTVRDAAPGVRVIDISGEITSFSEQDISAAHDEAARG
                     GVTAIILNFDGLEYMNSGGIGLLVTTLIRAQRNGHRLLAYGLSDHYRQIFSLTRLDEA
                     IGIFDDEDAALAAV"
     gene            complement(994..2388)
                     /locus_tag="AC161X_05985"
     CDS             complement(994..2388)
                     /locus_tag="AC161X_05985"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010048815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR4989136.1"
                     /translation="MTNFDAVVVGAGPNGLAAAAELTRAGRRVQVIEQADVIGGGTRT
                     EELTLPGFLHDICAAIHPLGLASPFFRDLGLDVEWIQPRIPMSHPLGEGRSGALLRDV
                     RETAAGFGVDADRYSRIMSPLVESADAVIEDFLGPMTMIPRNPSSFIRLATRGALPAS
                     TIAGGFSSMEARGVLAGLAAHAIAPFSSPLTGGVALLFATAGHAYGWPLVRGGSQRVA
                     EALARVIVDGGGAIETGRMIASLDELPPVATVILDVMPRAALHIGGDRISPIHRRRLS
                     GQQSGPAVFKVDWALDGAIPWLDEWSTEAGTVHVGGTWEEVAEAEDAVHSGRLPERPF
                     VLVAQQSLFDETRAPAGKQSAWGYCHVPAGSDVDMTEAIERQIERFAPGFRDLILGRH
                     TMNASAFEAHNPNNIGGDIAGGGFGMRKVFQLGATRPYHLGDGLYLCSSATPPGAGVH
                     GMCGYYAARAALRG"
     gene            2514..3203
                     /locus_tag="AC161X_05990"
     CDS             2514..3203
                     /locus_tag="AC161X_05990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1990 domain-containing protein"
                     /protein_id="HIR4989137.1"
                     /translation="MTEPRDAENWAEPVDAFHVGEVADGARKGNVEGRKPTGPLQGFG
                     QLWQKSYEVSVPGPTPEVIIATWKENFDEFWPPSNKFYAPAGGIAPGEVAVIGGGKGP
                     AKVTTGVRVIYADDRSWSYMTPEGHPWAAIITFSSHEGDDDAAVAKIHLLVRANDPLY
                     EASFKLYTSRLEDKIWTHTLTQLANHFGVTEPDVIATAALIDKKRQWNQMGNIWKNSA
                     IRTLLGQDRKG"
     gene            complement(3275..3811)
                     /locus_tag="AC161X_05995"
     CDS             complement(3275..3811)
                     /locus_tag="AC161X_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989138.1"
                     /translation="MTAIETDRGTTKVIGAPRTTLVLLIAGILAMVVGFGSIAGGIFG
                     AWYTYDHAVAQDVVTPDDAAIPETPVRGPFTMWAQADIINHHQLESTGGLYYAQMDRT
                     VPQLDESGAAVLDANGEAVMVPNEARASWITATTLTTALGMGIMAYALSGFAMAVGVT
                     LMVVGFVFLYLRKKAVLL"
     gene            complement(3933..4712)
                     /locus_tag="AC161X_06000"
     CDS             complement(3933..4712)
                     /locus_tag="AC161X_06000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HIR4989139.1"
                     /translation="MRLRVTSIGLTVLGLLLVGCGAGEDVVTTSLPSTTTSTTSSPES
                     TTTNSLPVTTTTTVAESTTTLSSGLPGDPIDFGPAEGDVLGVIGVAHDDVLNLRAVPG
                     ADQAILVGIPPLFSDLIALGSTRQLAASMWIEVDYSGLTGWVNLRFIAYLGATSDVTA
                     DVVSSLGETPVAPTMPELGLLVAEHLVADNPDASFVMSGPATVGDLGEVTYDIVGFGD
                     DSVRGTRIHVFGEPVDDGFALNAVEATPFCSRGVTPDGVCL"
     gene            complement(4771..5577)
                     /locus_tag="AC161X_06005"
     CDS             complement(4771..5577)
                     /locus_tag="AC161X_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005439079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HIR4989140.1"
                     /translation="MDYQHLEIEKAGHVATIWLNRPEKLNAMSEDIWVDLPRVVAELD
                     SDESVRVMILAGRGRSFSVGIDIEMLASLLPSGPSDDPQEALYSQIKTLQVTISCLAE
                     SPKPVIAAVHGHCLGGGMGLITGCDVRYGSADSVFSIRETRLAVVADIGTLQRLPGIV
                     GAGHTNELALTGADIDAARAVEIGLLNRVYPDAAATYEAARDLAGTIAANSPLVIQGI
                     KKVIAANDGRSVPEALDFVARWNAEHLLSNDLMEALAALVAKRPPEFTGT"
     gene            complement(5580..>6077)
                     /locus_tag="AC161X_06010"
     CDS             complement(5580..>6077)
                     /locus_tag="AC161X_06010"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989141.1"
                     /translation="GLAQFAGTIIVAFLTLRVTGAYDIPPVDMTLVAHLVLWFTLGFL
                     LYAVLFGAAGSLVSRMEDAQNVAFPMSLIAVAGFLVAITSLSDPDGVTATIGTFIPLT
                     APFVVPVRVALDAIPAWQYIVAVLLTVASIIGLVLVAGRIYAGGLLRYGSRVKVREAW
                     RSAAE"
BASE COUNT         1193 a         1901 c         1960 g         1023 t
ORIGIN      
        1 gccatcgagc acggaaacga gaatcgcgcc gagatccctg tcgagatcga ggttcgggct
       61 acggccgcca gtgtctcggt ccgggtcagc gaccagggcg gtgacaaggt cattcccgag
      121 gccgaaaccc ctgacatcga ggcgaaattg gcgggcgagc agacgccgcg gggctggggg
      181 ctgttcctga tcggccagat ggttgatgag atcaaaacag agagcaacga caacatccac
      241 acggtggagc ttgtcatgaa cagagaagga gtctcctgat ggacgacggc ttcggagcga
      301 gggtctcctg gggcgacgac gggggagttg tgcatctgac cgggacagtg aaccgcctcg
      361 ccaaggatgg cctggagagt gcatacgagg aggcgacctc gaaaccgggt cgcgttttga
      421 tcgacttcgg ggaggtcgac tacatcaact ccaccggaat tgcagtcatc gtcggtgtgc
      481 tcgccagggc gcgggctgag ggtcgcgagg tgggcgcatt cggcctctca gatcattacc
      541 gcgaggtgtt tcagatcacc cggctcgccg acttcatggc catctacgaa gacgaagacg
      601 ctgcctcggc agccagataa ctggagacat aggtgccaac gtccaacctg accatgactg
      661 tccgagatgc cgccccggga gtgcgcgtga tcgacatctc gggcgagatc acatcattct
      721 ccgaacagga catttctgct gcacacgacg aggccgctcg cggcggcgta acggccatca
      781 tcctcaactt cgacggcctg gaatacatga acagtggcgg tatcgggctc ctggtcacca
      841 ctttgatccg tgcccagcgc aatggccacc gtctcttggc ctacggcctc tccgaccact
      901 atcgccagat cttctccctg acccgcctcg atgaggcgat tggcatcttt gacgatgagg
      961 atgctgccct ggctgcggtc tgatccgcgg gcctcaaccc ctgagcgctg cacgagcggc
     1021 gtagtaaccg cacatgccat gcacaccggc cccgggcggg gtcgccgacg agcagagata
     1081 gaggccgtcg ccgaggtgat agggccgtgt ggcgcccaac tggaacacct tccgcatccc
     1141 gaaaccgcca ccggcgatgt cgccgccgat gttgttcggg ttgtgcgcct cgaacgccga
     1201 tgcgttcatc gtgtggcgac cgagaatcag gtctcgaaag ccgggagcga agcgctcgat
     1261 ctggcgttcg atggcttcgg tcatgtcgac gtcagagcca gccggcacgt ggcagtagcc
     1321 ccatgccgac tgcttgccgg ccggagctcg ggtctcgtcg aacagcgact gctgagcgac
     1381 caaaacgaaa ggacgctcgg gaaggcgacc cgagtgcact gcatcctcgg cttccgccac
     1441 ttcttcccag gttccgccca catggaccgt tcccgcttca gtgctccatt cgtcgagcca
     1501 ggggattgcg ccatccaggg cccagtccac cttgaacacc gcagggccgg actgctgtcc
     1561 tgaaagacgc cgacggtgaa ttggactgat gcggtcgccg ccgatgtgaa gggcggcacg
     1621 gggcatcacg tcgaggatca cggttgcgac cggggggagc tcatccaggg aggcgatcat
     1681 cctcccggtc tcgatggctc cacctccgtc gactatcacc cgagccaatg cctccgcgac
     1741 tctttgtgat ccgcccctca ccagcggcca tccataggca tgtccggcgg tggcaaagag
     1801 cagggcgacc ccgccggtca acggcgagct gaacggggcg atggcgtggg cggccagccc
     1861 ggccaaaaca cctcttgcct ccatcgagga gaacccaccc gcaattgtgg aagccgggag
     1921 ggctcccctg gtcgcgagcc gaataaagct cgaagggttg cgggggatca ttgtcatcgg
     1981 ccccaggaag tcctcgatga cggcatcagc cgactcgacg agagggctca tgatccggga
     2041 atagcggtcg gcatcgactc cgaaccccgc ggctgtttca cggacatcgc gaagcagggc
     2101 gcctgaccgc ccctcgccaa ggggatgaga catcgggatt cgcggttgga tccactccac
     2161 gtcgagacca aggtcccgaa agaatggcga cgccaggcca agggggtgga tcgcggcgca
     2221 gatgtcgtgg aggaagccgg gcagcgtcag ctcttcggtt cgggtccctc caccgatcac
     2281 atccgcttgt tcgattacct ggacccgtct gccggcacgc gtcaattcgg cggctgcggc
     2341 caggccgttg gggccggcgc cgacgaccac cgcgtcgaag ttcgtcatct gaggtgattg
     2401 tgcatcaatt gcgttacgag acggtgaccg ctcgaagccc tcgcccaatc gagtttcgta
     2461 acgctcacgg aacgcgtctc tggtcggatg cgggtatcaa agaaaggacc gagttgacag
     2521 agccgagaga tgctgagaac tgggccgaac cggtagacgc ctttcatgtc ggggaagtcg
     2581 ccgacggtgc ccggaagggg aatgtcgagg gtcgcaagcc caccggccct ctgcagggct
     2641 tcggccagct ctggcagaag tcttacgagg tctcggttcc cgggcccacc ccggaagtca
     2701 tcatcgcgac ctggaaggaa aacttcgacg agttctggcc cccctccaac aagttctatg
     2761 ccccggccgg gggcatcgcc ccgggtgagg tggcggtgat cggcgggggc aagggcccgg
     2821 ccaaggtcac gaccggggtc cgcgtgatct acgccgacga tcggtcctgg tcgtacatga
     2881 cccccgaagg ccacccctgg gcggccatca tcaccttcag ctcccacgag ggtgacgacg
     2941 acgccgcggt cgccaagatc cacctcctgg tccgggccaa cgacccgctg tacgaggcca
     3001 gcttcaagct gtacacctca cggctcgaag acaagatctg gacccacaca ctgacccagc
     3061 tcgccaatca cttcggcgtg accgaacctg acgtgattgc gacggcggcc ttgatcgaca
     3121 agaagcggca gtggaatcag atggggaaca tctggaagaa ctcagcgatc cggaccctcc
     3181 tcgggcagga ccgaaagggc tagcggctcg agcaagggcc ctttacgaat cgagccccct
     3241 tccgggggct cgattcgatt gctgcgactc tgagtcagag gagcacagcc ttcttccgca
     3301 ggtagaggaa cacgaacccg acgaccatca gcgtcactcc gacggccatt gcgaagccgg
     3361 agagggcata ggccatgatt cccatgccga gtgcggtggt cagggtggtg gcggtgatcc
     3421 aggaggcacg ggcctcgttg ggaaccatca cggcctcacc gttggcgtcg agcacggcgg
     3481 cgccgctctc gtccaactgg ggcaccgttc ggtccatctg ggcgtagtag aggcctccgg
     3541 tcgactccag ctggtggtgg ttgatgatgt cggcctgcgc ccacatcgtg aacggccctc
     3601 tcaccggtgt ctcgggaatc gcggcgtcat ctggcgtcac gacgtcttgg gccactgcgt
     3661 ggtcgtaggt gtaccaggcg ccgaagatgc ctccggcaat ggagccgaac ccaacaacca
     3721 ttgccaggat tccggcgatg aggagaacca gagtcgtgcg cggagcgccg attaccttgg
     3781 tggtgcctct gtcggtctcg attgcagtca tcgctgcctc cttctatgag aattaactgc
     3841 ctatatggcc atgttcggcg tcatgtggcc tggatacgag aggactggtc ccctaatcgg
     3901 tgggccataa ggcccggcac ccgagaggtg ggttagaggc agacaccgtc cggtgtgacg
     3961 cctctgctgc agaatggggt tgcctcgacc gcattgaggg cgaagccatc gtcgaccggc
     4021 tcgccgaaca cgtggattcg cgtgcccctt accgagtcat caccgaaacc cacgatgtca
     4081 tatgtcacct cgccgagatc acccacggtt gccggaccgg acatgacaaa tgaggcatcg
     4141 gggttgtctg cgacgaggtg ctcggcgacg agcaggccga gctcgggcat ggtcggtgcg
     4201 acgggtgtct cccccaggct gctcacgacg tctgcggtga catcgctggt agctcccaga
     4261 taagcgatga aacgcagatt gacccagccg gtgagccccg agtaatcgac ttcgatccac
     4321 atcgacgccg ccagttggcg ggtagagccg agcgcgatca ggtcggagaa caggggcggg
     4381 attccgacca ggatcgcctg atcagctccg ggaactgccc tgaggttgag cacgtcgtcg
     4441 tgggcgaccc cgatcacgcc gaggacgtca ccttccgcag ggccgaaatc gatggggtct
     4501 cctgggagac ctgaggacag agtcgttgtc gattcggcca cagtggtggt cgtcgtaacc
     4561 ggaaggctgt tcgtcgtggt cgattccgga gacgaagtcg tgctggtcgt agtcgagggc
     4621 agcgaggtgg ttacgacgtc ttcaccagca ccgcatccga ccagcagaag gccgagcacc
     4681 gtgagcccta tagatgtcac ccgcagtcgc aatcgaaact cctcaccgcc gcaggaggca
     4741 acgtaccgcg ccagacgccg ctcccgggcc tcaggtgcct gtgaattcag gcggtcgttt
     4801 cgcgaccaac gcagccagcg cctccatcag gtcgtttgag agcaggtgct ccgcattcca
     4861 gcgcgcgaca aagtcgagcg cctcgggaac cgatcgccca tcgttggcgg caatgacctt
     4921 cttgatccct tgtatgacga ggggcgaatt ggccgcaatg gtgcctgcca ggtcacgcgc
     4981 cgcctcgtag gtcgcggcgg cgtccgggta gacccggttg agaagcccga tctcgacggc
     5041 tcgcgccgca tcgatgtcgg cgccggtcag ggccagttca ttggtgtggc cggctccgac
     5101 gattccaggg agtcgttgca gggtcccgat gtcggcaacc accgccagcc gcgtctcccg
     5161 aatcgagaac accgagtcgg ccgacccgta gcggacatcg catccggtga tcaggcccat
     5221 gcctcccccc agacagtgcc catgcaccgc ggcgatcacg ggtttggggg actcggcaag
     5281 acagctgatc gttacttgaa gggtcttgat ctgggagtaa agagcctcct gagggtcatc
     5341 cgaaggccca gacggcagaa gggaggcaag catctcgata tcgatcccaa ccgagaacga
     5401 acgtccacgc cccgccagaa tcatcacccg gaccgattcg tcgctatcga gttcagccac
     5461 cacccgggga aggtcgaccc agatgtcctc ggacatggcg ttcaacttct ccgggcgatt
     5521 cagccagatg gtcgcgacat gacccgcctt ttcgatctcg aggtgctggt agtccacact
     5581 cactccgcgg cggagcgcca ggcttcccgg accttgaccc ggctcccgta gcggagcagg
     5641 ccaccggcgt atatccgtcc ggcaacgagg acgagcccga tgatcgatgc cacagtcagt
     5701 agcacggcca cgatgtactg ccaggccgga attgcgtcca gtgctacccg caccggcacc
     5761 acgaagggag cggtcaaggg gatgaaggta ccgatggtgg cggtgacgcc gtcagggtcg
     5821 gaaagagatg tgatcgcgac caggaacccg gcgaccgcga tcagggacat cgggaaggcg
     5881 acgttctgag cgtcctccat ccggctcacc agggagcccg ccgccccgaa gaggacggcg
     5941 tagaggagaa aaccgagagt gaaccagagc acgaggtggg cgaccaaagt catgtccacc
     6001 ggaggaatgt cataggcgcc cgtgacccgc agcgtgagga aggcaacgat gatggttccg
     6061 gcgaactggg ccagccc
//
LOCUS       DBNHXU010000241         2339 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2458218, whole genome shotgun sequence.
ACCESSION   DBNHXU010000241 DBNHXU010000000
VERSION     DBNHXU010000241.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2339)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2339
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2458218"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /gene="cofD"
                     /locus_tag="AC161X_06015"
     CDS             <1..902
                     /gene="cofD"
                     /locus_tag="AC161X_06015"
                     /EC_number="2.7.8.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0043743 - LPPG:FO 2-phospho-L-lactate
                     transferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="2-phospho-L-lactate transferase"
                     /protein_id="HIR4989142.1"
                     /translation="SELSGGVGGARLARGLARLENVDLTLIVNVGDDELVHGLSISPD
                     LDTVLYTLAGLEGPEGWGRRDDTFVTNDEIGRFGLDNRFRLGDLDLALNLYRSSRLSA
                     GDTLTEITAALRRRFELKPLVLPASNDELRTEVRLGAEDWISFQEYFVFRQHRDEVTQ
                     LRFRGSSEARPAPGVLESLEAAEVVLIAPSNPPLSIWPILAVPGIQDAVANHPRVVAV
                     SPLFGGRALKGPADRVMASLGLPPGNPGVAQAYDGLIDALVVDARDEAESGSIEGVEV
                     IVADTLITDPEASARLSAAILGL"
     gene            899..1597
                     /gene="cofE"
                     /locus_tag="AC161X_06020"
     CDS             899..1597
                     /gene="cofE"
                     /locus_tag="AC161X_06020"
                     /EC_number="6.3.2.31"
                     /inference="COORDINATES: protein motif:HMM:TIGR01916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043773 - coenzyme F420-0 gamma-glutamyl
                     ligase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coenzyme F420-0:L-glutamate ligase"
                     /protein_id="HIR4989143.1"
                     /translation="MNLEILPVMGIGPVSPGDDVAGMLESALESLGPVSGDVLVVTHK
                     IVSKAEGAVVQIEGDEEEFRKRLIESEAVSIIRRRGDLIIAETKHGFICANAGIDRSN
                     AAPGTMILLPEAPDRSAHRIRMRLERHFGIELPVIISDTFGRPWRKGLTDVAIGLSGV
                     EAILDLKGTADWTGHELEVTEVAVADELAAAADLVMGKAAGIPAALIRGYTGPRGNGR
                     GRDLVRPAEEDLFR"
     gene            complement(1674..>2339)
                     /locus_tag="AC161X_06025"
     CDS             complement(1674..>2339)
                     /locus_tag="AC161X_06025"
                     /inference="COORDINATES: protein motif:HMM:TIGR03026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR4989144.1"
                     /translation="NNAYLATRLTFVNTLANVCEAVGADIIDVVRGIGLDHRIGPHFL
                     QPGPGYGGSCFPKDTSALIAVAADAGYDFELLRAVIEADEEQRRRVAEKVRQAAGGGL
                     HGRRIAMWGVAFKAGTDDLRESPAVRIAALLQADGAEVVAYDPEASTDQVTMAPDPLT
                     AATDADVLLIATEWPEFTAVDMSEVARVMRGHRVVDARNLLDPKAVRAVGLDYWGLGR
                     PGA"
BASE COUNT          438 a          723 c          752 g          426 t
ORIGIN      
        1 tcagcgagct ctcaggcggg gtcggtggtg cccggctcgc gagaggtcta gcccgactcg
       61 aaaatgtaga tttgaccttg atcgtgaacg tcggagacga cgagttggtc cacggtctga
      121 gcatctcgcc ggatctcgac acggtgctct acaccctcgc tgggctcgag ggacccgaag
      181 gctggggacg tcgggacgac acatttgtca ccaatgacga gatcggcagg ttcggcctgg
      241 acaatcgatt tcggctcggt gatctagacc tcgccttgaa tctctatcgg agtagccgac
      301 tctccgccgg agacacactg accgagatca cggccgcact ccggcgtcga ttcgagctca
      361 aaccgcttgt tctccccgcg agcaacgacg agctgcgaac ggaggtcagg ctcggggccg
      421 aggattggat ctcctttcag gagtacttcg tctttcgcca gcatcgcgac gaggtgaccc
      481 aacttcggtt ccgaggatcc tccgaagctc gacccgcacc tggtgtcctg gaaagcctcg
      541 aggctgcaga agtggtgctt atcgctccct cgaacccacc cctctctatt tggccaatac
      601 tcgcggtgcc cggcatccaa gacgctgtgg ccaaccaccc acgagtcgtg gccgtcagcc
      661 ctctgttcgg gggtagggcg ctcaaggggc cggccgaccg tgtcatggcc tcgcttggcc
      721 taccgccggg caaccccggt gtcgcccagg cgtacgacgg cctgatcgat gccctcgtcg
      781 tggacgctcg agacgaggcc gaatcgggct ctatcgaggg ggtcgaagtg atcgtcgccg
      841 acaccttgat cacggatccg gaggcgtccg ctcgattgag cgcggcaata ctcggtctgt
      901 gaacctcgaa atcctgcccg tgatgggaat cggtcccgtg tccccgggcg acgatgtggc
      961 agggatgttg gaatcagctc tggagtccct gggtcctgtt tcgggcgacg tgctggtggt
     1021 cacccacaag atcgtctcca aggccgaggg cgcagtcgtc cagatcgagg gcgacgagga
     1081 ggagttcaga aagcgcttga tcgagtccga agccgtctcg atcattcgaa gacggggcga
     1141 tctgatcatc gccgagacca aacacggctt catttgcgcc aacgcaggga tcgatcggtc
     1201 caatgccgca cccggcacga tgattttgct ccccgaagct ccggaccggt cggcacatcg
     1261 gattcggatg agattggagc gccatttcgg gatcgagttg ccggtcatca tctccgatac
     1321 gtttggccgc ccctggcgca agggtctgac cgatgtcgcc atcgggttgt cgggagtcga
     1381 ggcgatcctc gatctgaagg ggacagccga ctggacgggg catgagctcg aggtcacgga
     1441 ggtggcggtg gcggacgagt tagccgccgc agccgatctg gtgatgggca aggcggccgg
     1501 gatccccgct gcgttgatcc ggggctacac cgggccgcgt ggaaacggaa gggggaggga
     1561 cctggtgagg ccggccgagg aggacctgtt ccgctgaaaa ggtcgcaagt cctaagtctc
     1621 aagtctcaag ccagaccttt ctggcttggg acctatgacc tgagacctgg gacttaggcg
     1681 ccgggccttc ccagacccca ataatcgagc ccgaccgccc ggactgcctt cggatcgagc
     1741 aggttgcggg cgtcgacaac gcggtggcct cgcatgaccc gagccacctc cgacatgtcg
     1801 acagccgtga actccggcca ctcggtcgca atcaaaagca cgtcggcgtc ggtggccgcc
     1861 gtcaatgggt cgggggccat ggtgacctgg tcggtcgaag cttcgggatc gtatgcaacc
     1921 acctcggcgc catcagcctg cagaagtgca gcgatgcgaa cggcgggcga ctcacgcaaa
     1981 tcatcggtgc cggccttgaa ggcaacgccc cacatcgcaa tccggcgtcc atggagcccg
     2041 ccaccggcgg cctgcctgac cttctcggca acccgacgtc gttgttcttc gtcggcttcg
     2101 atgactgccc gcagcagctc aaagtcgtaa ccggcgtccg ctgcaaccgc aatgagagcc
     2161 gaagtgtcct tcggaaaaca ggaaccccca tacccgggtc cgggctgcag gaagtgtggg
     2221 ccgatcctgt gatcgaggcc aatgccgcga accacgtcga tgatgtcggc accaactgcc
     2281 tcacacacat tcgccaacgt gttgacaaag gtgagacggg tggccaggta ggcgttgtt
//
LOCUS       DBNHXU010000242         9587 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3449805, whole genome shotgun sequence.
ACCESSION   DBNHXU010000242 DBNHXU010000000
VERSION     DBNHXU010000242.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9587)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9587)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9587
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3449805"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..394
                     /locus_tag="AC161X_06030"
     CDS             <1..394
                     /locus_tag="AC161X_06030"
                     /EC_number="2.8.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0000098 - sulfur amino acid catabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sulfurtransferase"
                     /protein_id="HIR4989145.1"
                     /translation="SVSYPEASNFSGVARIDDLAGRQLIDARAAERYRGEVEPVDPKA
                     GHIPGAINMPTSGNLSPAGSFLTPGELVELYQETPDDAIVSCGSGVTACHAALAMVLA
                     GHAMPDVYIGSFSDWSRRDLPVVTGDLP"
     gene            391..1350
                     /locus_tag="AC161X_06035"
     CDS             391..1350
                     /locus_tag="AC161X_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989146.1"
                     /translation="MISQRAFEAIESSNTDELLRVIDGLCSAQAWDDLVELRARCREA
                     VGRAKQIWGVEEHIRFRLALESPPVFAGPVVSEGQARFALGPLPEVAASTKTWADIEP
                     YLDMGPERRTFAAERVVRGEDVPEAGLDLPGSLQPWEPGYQLATYKRDKVEAPSPPPA
                     TVTETPLPATVDLLDDPQSEAALADLVQPWTDESNGRCQVTVVTGDHLAAIRALGATM
                     CLEADIGTQLGLAWMGWAAASGGAHGRRRGAAAGRHLAWWATSVLADLSWPCAPDELG
                     AAASRFRWYWFDEGSPSTGWALRLAIEDPENDLSWAVSAVDLM"
     gene            complement(1423..2964)
                     /locus_tag="AC161X_06040"
     CDS             complement(1423..2964)
                     /locus_tag="AC161X_06040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018784628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR4989147.1"
                     /translation="MSSTRSKDALSGAAAAALALGVSELAAGIFSSLPSLIEGVGNWV
                     IDIVPESIKHWAISVFNTNDKLALLVGMTLVVILLGGLVGMAARNHFGVAMAAFIGFG
                     VLGALAASREPAVSLGTAMISGGLAALTGLVSLQWLLGLDPPVAVAEGVEQSANPSRR
                     SFIFGVSAVLGLAALSAGVGRVLLERGKVILAGREQVVLPAAVEAVAPVPAAAEFTGI
                     SGLSPVVTPNASFYRIDAAFSVPRVDLPEWTLSIKGMVDRPYSINYADILDMPMVERY
                     VTLSCVSNQVGGDLVGNAKWLGVPLTEILDRAVVQPDAEQIVGRSVDDFTVGFPVDVA
                     YDGRDALLAVGMNDEPLPFEHGFPARLVVAGLYGYVSATKWISEIELTRWDDFDAYWV
                     PRGWAKEAPVKTQSRIDTPASGSQVPMGPRQVAGVAWAPNRGISRVEVQLGEDSPWLD
                     AELSEPLSDNSWRQWSIEWDAPLGTHRIRVRATDGEGEVQTEAIVPPAPNGASGWHTI
                     ETIVA"
     gene            3037..3645
                     /locus_tag="AC161X_06045"
     CDS             3037..3645
                     /locus_tag="AC161X_06045"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006726577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HIR4989148.1"
                     /translation="MMDLIAGDDGLARCWWGAEPEIYRGYHDTEWGYPVTEDRRLFEK
                     ICLEGFQAGLSWLTILRKRPNFRTAFANFEIEQVAVFGELDVARLLGDEGIVRHQGKI
                     RSTVNNAQRALDLVEEKGTLAAYFWEWADPADVPPSDIAPTTPTSTSLSKDLKKRGWT
                     FVGPTTVYAFMEAMGLVNDHLDGCHARPKCAEERQRLLGSGL"
     gene            3656..4789
                     /locus_tag="AC161X_06050"
     CDS             3656..4789
                     /locus_tag="AC161X_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012973231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0042398 - modified amino acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylate-amine ligase"
                     /protein_id="HIR4989149.1"
                     /translation="MLDRPAFTIGIEEEYLVVDRESRDLIKNPPSEMWDSLREVLGPQ
                     VTPEFLKAQIEVGTKVCTKIQEAREDLANLRRDLSGVVSEYGAAIIAASTHPFANWSE
                     QMTTEHPRYQRLAADHQQVARQLVICGMHVHVGIEDEHLRIDLMNQIRYMLPHLLALS
                     TSSPFWAGTDTGLLAYRLIIFRNLPRTGPPEEFESWAEYQRTMQILIDAGIIEDASKV
                     WWDIRPSSRYPTLEMRVSDVCTRLDDAMSVAALYLCLLGFMFRLRRRNQRWRVYSPFL
                     ISENMWRAQRYGVDGSLIDFGRGELIPFSELVEEAIEMTAQDAVEFDVVDQMRHMRTI
                     VREGTSAHRQLAVYRQALEGGAEEAEALTAVVDHLIEDTLFGI"
     gene            4835..5617
                     /locus_tag="AC161X_06055"
     CDS             4835..5617
                     /locus_tag="AC161X_06055"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005458348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR4989150.1"
                     /translation="MARPIRIAVQLQPQHAEYAEIRAAVVQAEEMGADLVLNWDHFYP
                     LSGDPDGKHFECWTMLASWAEITERVEIGALVACNSYRNPDLLADMARTIDHISEGRF
                     ILGIGAGWFERDYDEYGYEFGTAGSRLADLKSALPRIEARWSKLNPAPTRKIPVMIGG
                     GGEKKTLRYTAQHADIWHGFGQAEIIRHKNGILDAWCRELGRDPSEIERSCGANARKP
                     ESGDAFVEAGANLITLGFDGRSGYDLEPVTEWLAWRDEKNRV"
     gene            complement(5622..6494)
                     /locus_tag="AC161X_06060"
     CDS             complement(5622..6494)
                     /locus_tag="AC161X_06060"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020018908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HIR4989151.1"
                     /translation="MLAAFTYPPIPIWDLGPISFSLHGLFAALGFVAGAWIATREMAK
                     RGFDTVKYQSCLTWGLIGALLGARYFTAPAQIMDGTPLLEALNPIAGNFSIMGGFLGG
                     ILAGGWRAHRLKMPLWATLDMSAFGLALGTVVGRIGDLAIVEHLGRATTAWWGYGIRP
                     GYDVAPQHDGLECTEAAVGANGFCGIYHHVALYDMIGAAVLLWVLFYVYRHWRLHYGQ
                     LFFIWVAWYGFQRFLLDGLRFGMGDAVVGGFTWNQLSGFLAGLGGVVMIWWLDRKEEF
                     VTPENDRQLIGASA"
     gene            6606..7763
                     /locus_tag="AC161X_06065"
     CDS             6606..7763
                     /locus_tag="AC161X_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989152.1"
                     /translation="MSDLLPTVAAALNTPEALIKRSAAARAAANGTTIDEVLAAWAGG
                     APVVAAAPAQAPEPAVAQTTTDPDPVPEPVATPTTITPAPVAVIVEPEPEPSVPLEPV
                     PISKRISSATRVGAWTGAVLGLTGFLVATTWWAQNATVIGEGPFTPVIQTTSRTVIIG
                     AALVSILFGAITAGLSRASASWANPGMQLRNSTSSTGWLGALIGLVLGIGAAALLTGG
                     FGTPVEGAEGMVQLPVLPTLAVMLLGGAALGAITAAITQAVAVPVTVDAGEVADVRSR
                     LGGALTIPAIALILLVILVLPFAWGLIQSAHITKEAAALVAIITAIGILGFASLAGTR
                     PNVKISFGEAMIALIGIVTVLIIVFAVLFARSPGEEPEEAGQEAAAVSILA"
     gene            complement(7787..8740)
                     /locus_tag="AC161X_06070"
     CDS             complement(7787..8740)
                     /locus_tag="AC161X_06070"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HIR4989153.1"
                     /translation="MKGQFRVVVTDHVSDSGLAPLLDDARFEVIRIDDSSTDEFRAAL
                     AGADGLVVRSATKVTREMLSGGPRLKVVGRAGVGVDNIDLNAATERGIAVLNSPAGNT
                     VAAAELTVALILSVVRRVAEADRSMRAGKWERAKLRGVQLKDRTLGLVGAGRIGGEVA
                     VRCRAFGMKVIVYDPYLTAERADDLGLALTDLDEVLRQGDIISLHVPLNDETRHLIDT
                     VAISKMKPGAFVVNVSRGGVIDEEALARALVDGHLGGAALDVFESEPLSPESPLLDLP
                     NVVLTPHLGASTVEAQELVAREIAESVREALALGDISGSVT"
     gene            complement(8737..>9587)
                     /locus_tag="AC161X_06075"
     CDS             complement(8737..>9587)
                     /locus_tag="AC161X_06075"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal-phosphate-dependent aminotransferase
                     family protein"
                     /protein_id="HIR4989154.1"
                     /translation="KVLSLVNGAFSKRFADIATACGRDVDIWEVPWGEYHEPGELAGR
                     LASGDYDAVTVTHSETSTGVLQDLEALAGVVAREDDTLFLVDSVTGVCGAEVRSDDWS
                     LDWVLTGSQKAMALPPGLAFGVASEALLKRSATLADKGVYFDLVALTQSLEVNQTPAT
                     PAISLMYALRLQLERILAEGIEARWKRHTAMRERALEWVDEMSGLGLAPFAPEGYRSP
                     TITCVSLPERITGPEVAKGMKDRNWVIGSGYGKLKETTIRIGHMGDRTVDELDLLLQD
                     LGDVIR"
BASE COUNT         1805 a         3151 c         2991 g         1640 t
ORIGIN      
        1 gtcggtctcc tatcccgaag cttcgaattt cagcggtgtg gcccgcatcg acgacctggc
       61 cggacgccag ctcattgatg ccagagccgc cgaacgttat cggggagagg tcgagccggt
      121 ggatcccaaa gcgggccaca tcccgggggc gatcaacatg ccaacgagcg ggaacctcag
      181 tccggcagga agtttcctga ccccgggcga gctggtcgag ctctatcagg agacgcccga
      241 cgatgcgatc gtgtcatgcg ggagcggggt caccgcctgc catgccgctc tggcaatggt
      301 ccttgccggc catgccatgc ccgacgtgta catcgggtcg ttctcggact ggtcccggcg
      361 cgacctaccg gtggtgacgg gcgaccttcc ttgatcagcc agagggcctt cgaagccatc
      421 gagtcgtcga acaccgacga attgcttcgg gtgatcgacg gtttgtgctc ggcccaggca
      481 tgggacgact tggttgagct tcgagccagg tgccgggagg cggtggggcg agccaagcag
      541 atctggggag ttgaggagca catccgtttt cggttggcgt tggagtcgcc tcctgtcttc
      601 gccgggcccg tagtgtctga gggtcaggcg cgattcgccc tcggccccct gcctgaggtg
      661 gccgccagca ccaagacctg ggcggacatc gagccgtatc tggacatggg accggagcgg
      721 cgaacgtttg ccgcagaaag ggttgtgcgc ggcgaggatg tgcccgaagc aggtctcgat
      781 ctgcccggct ccctacagcc ctgggagccc ggttaccagc tagccactta caagcgtgac
      841 aaggtggagg cgccgtcgcc gccgcccgcc accgtcacgg agacaccgct accggcgact
      901 gtcgatttgc tcgacgaccc acaatcggaa gccgccctgg ccgatctcgt ccagccttgg
      961 accgacgaat cgaacgggcg gtgtcaggtg acagtcgtca ccggcgacca cctggcggcg
     1021 atccgcgcac ttggcgcaac gatgtgcctg gaggctgaca tcgggacaca gttgggtctg
     1081 gcgtggatgg gctgggcggc cgcatcggga ggtgctcacg gaaggcgacg cggtgccgcc
     1141 gccggccgcc atctcgcgtg gtgggcgact tcggtgttgg cggatctctc ctggccgtgt
     1201 gcccccgacg aactgggcgc cgcggcctcc cgttttcgct ggtactggtt cgacgaagga
     1261 agcccgagca ccggctgggc cttgagactc gccatcgagg acccggaaaa tgatctctcc
     1321 tgggcagtct cagccgtgga tctgatgtga gggtcttggc cctccgtaat tgagctacac
     1381 aggtccgtat atgaccccta aacgcaacaa ttagggaagg ggtcaggcga cgatggtctc
     1441 gatggtgtgc cagccgctgg caccgttggg agccggcggg acaatcgcct cagtctggac
     1501 ctctccttct ccatccgtgg cgcgcacccg aatccggtgt gtgccaaggg gggcgtccca
     1561 ttcgattgac cactgtcgcc aactgttgtc ggagagcggt tcggagagct cggcatccag
     1621 ccacggtgag tcttcgccga gctgcacctc gaccctggag atgccccggt tcggcgccca
     1681 ggcgacgccc gccacctggc ggggtcccat cggcacctgc gaaccggaag ccggagtgtc
     1741 gatacggctc tgggtcttga ccggcgcctc cttggcccaa cctcgcggca cccaataggc
     1801 gtcgaagtca tcccaacggg tgagttcgat ctccgagatc cacttggttg ccgagacata
     1861 cccatagagc ccggcaacga caaggcgagc cgggaaccca tgctcgaacg ggaggggctc
     1921 gtcgttcatg ccgacggcaa gcagcgcatc acggccgtca tacgccacgt cgaccgggaa
     1981 cccgaccgtg aaatcgtcca cagaccggcc aacgatttgc tcggcatcgg gttgcaccac
     2041 ggctcgatcg aggatctcgg tcagaggcac accaagccat ttcgcattgc cgaccagatc
     2101 gcctccgacc tggttcgaaa cgcaggacag agtcacgtac cgctccacca tcggcatgtc
     2161 gaggatgtcg gcatagttga tggagtaggg acgatcgacc atgcctttga tcgacaatgt
     2221 ccattccggc aggtcgactc ttggcaccga gaacgcggcg tcgatccggt agaacgatgc
     2281 attgggtgtc accaccgggc tcaagcccga gatcccggtg aattccgcgg cggcaggcac
     2341 cggtgccacg gcttcgacag cggcggggag cacgacttgc tcccgccccg ccagaatcac
     2401 ctttccccgc tccaggagaa cgcgtccaac acctgcggag agggccgcca gacccagaac
     2461 cgcgctgaca ccgaatatga aggagcgccg ggacggattt gcggactgtt cgaccccttc
     2521 tgccacagca acgggtgggt cgagcccgag gagccactgc aacgagacca gcccggtgag
     2581 cgccgccaac ccgcctgaga tcatcgcggt gcccaggctc acggccggct ctcgactcgc
     2641 agccagcgcc ccgagcactc cgaacccgat gaaagcagcc atggccactc cgaaatggtt
     2701 ccgggcagcc atgccgacca agccgccgag gaggatcacc acgagagtca tccctacgag
     2761 aagggccagc ttgtcgttgg tgttgaagac cgaaatcgcc cagtgcttga tcgattcggg
     2821 aacgatgtcg atgacccagt tcccgacccc ttcgatcaac gagggaaggc tggagaagat
     2881 cccggcggcg agctcggaga cgccgagcgc cagggcggcg gctgcggccc cggacagggc
     2941 atccttcgat cgagtggagc tcaattgaca cctcagagta ggaagacccc ggataaaccg
     3001 acctaaaggc aagttcgttg ctggtagaac cagtggatga tggatttgat tgcgggcgat
     3061 gatgggttgg ctcgctgctg gtggggggcg gagccggaga tctatcgggg ataccacgac
     3121 acggaatggg gctatccggt caccgaggac cggaggctgt tcgagaagat ctgcctcgaa
     3181 gggttccagg cgggtctgtc ctggctgacc atcctccgca aacgccccaa cttccggacc
     3241 gctttcgcca acttcgagat cgagcaggtg gcggtctttg gggagctgga tgtcgcacgt
     3301 ctgctcgggg acgaggggat cgtccgccat cagggcaaga tccgctccac ggtcaacaac
     3361 gcccaacgag ctctggacct ggtcgaggag aagggaaccc tggccgccta tttctgggag
     3421 tgggccgatc cagccgatgt gcccccatca gacattgccc cgacgacccc tacctcgacc
     3481 tccctgtcca aggatctcaa aaagcgtggc tggacattcg taggcccaac cacggtctat
     3541 gccttcatgg aggccatggg actggtcaac gatcatctcg acggctgtca tgctcggccg
     3601 aagtgcgccg aagaacggca gcggctgctg ggctccggtc tctagattcg cattcatgct
     3661 cgatcgccca gcgttcacca tcggcatcga agaggagtac ctcgtcgtgg accgcgagtc
     3721 acgtgacctg atcaagaacc ctccttccga gatgtgggac tcacttcgtg aagtgctcgg
     3781 gccccaggtc acccccgagt tcctcaaggc tcagatcgag gtgggcacca aggtttgcac
     3841 caagatccag gaggcccgcg aggacctggc caacctgcgg cgtgacctgt caggtgtggt
     3901 gagtgaatac ggggcagcga tcatcgccgc ctcaacccac cccttcgcca actggtcgga
     3961 gcagatgacg acggagcacc ctcgatatca acggctggct gccgaccatc agcaggtggc
     4021 tcgacaactc gtgatctgcg ggatgcatgt gcacgtcggc atcgaggacg agcaccttcg
     4081 aatcgacctg atgaaccaga tccggtacat gcttccccat ctcctcgccc tgagcacctc
     4141 gtcacctttc tgggcaggga ccgacaccgg gctgctcgcc taccggctca tcatcttccg
     4201 caatctcccc cgcaccggcc cacccgagga gttcgagtcg tgggctgagt accagaggac
     4261 catgcagatc ctcatcgacg cgggaatcat cgaggacgcc tccaaagtgt ggtgggacat
     4321 ccgtccctcc tcccgctatc cgactctcga gatgagggtc agcgacgtgt gcaccagact
     4381 cgacgacgcc atgtcggtgg ccgccctcta cctctgtctg ctcggcttca tgttccggct
     4441 ccgccgccgc aaccagcgtt ggagggtcta ctcgcccttc ttgatttcgg agaacatgtg
     4501 gcgggcccag cgttacggtg tcgacggctc cctgatcgac ttcggccgtg gcgagctgat
     4561 tcccttctcc gaactggtcg aggaggcgat cgagatgacg gcgcaggacg cagtggagtt
     4621 cgacgtcgtg gaccagatga ggcacatgag gacgatcgtc cgagagggaa cgagcgccca
     4681 tcgccaactc gcggtttaca ggcaggccct cgaaggcggc gcggaggaag cagaggcgct
     4741 gacggcggtc gtcgaccact tgatagagga cacacttttc ggcatctgac cggccgcggt
     4801 accgtcaggc aatcctgcaa ccggaagaga acccttggcc cgccccatcc gcatcgccgt
     4861 ccagctccag ccgcaacacg ccgagtacgc cgaaatccgg gctgcggttg tccaggccga
     4921 ggagatgggc gccgacctcg tgctcaactg ggatcacttc tacccactga gcggcgaccc
     4981 tgatggcaag cacttcgaat gctggaccat gctcgcctca tgggcagaga tcaccgagcg
     5041 agttgagatc ggcgccttgg tcgcctgcaa cagctaccgc aaccccgacc ttctggccga
     5101 catggcccgc accatcgatc acatctcgga ggggcgcttc atcctcggaa tcggcgccgg
     5161 ttggttcgag cgggactacg acgagtacgg atacgaattc ggcaccgccg gctccaggct
     5221 tgccgacctg aagtctgccc ttccccggat cgaggcccga tggagcaagc tcaatccggc
     5281 gccaacccgc aagatccccg tcatgatcgg tggcgggggt gagaagaaga ccctgcgcta
     5341 cacggcccag catgccgaca tctggcatgg attcggccag gcggagatca tccggcacaa
     5401 gaacgggatc ctcgacgcct ggtgccggga attgggcagg gacccgtcgg agatcgagag
     5461 atcctgcgga gcaaatgcgc gcaagcccga gtccggagac gcctttgtcg aggccggagc
     5521 caacctgatc actcttggtt tcgacggccg gtcgggttat gacctcgagc cggtgacgga
     5581 atggttggcc tggcgagacg aaaagaaccg cgtctgagaa gtcaggcgga ggcgccgatc
     5641 agctgacggt cgttctcggg tgtgacgaac tcctccttgc ggtcgagcca ccagatcatc
     5701 accacaccgc cgaggccggc caggaatccg ctgagctggt tccaggtgaa gccaccgaca
     5761 acggcgtcac ccatgccgaa acggaggccg tcgagcaaga accgctggaa cccgtaccag
     5821 gccacccaga tgaagaagag ctggccgtag tgaagtcgcc aatggcggta cacatagaag
     5881 agcacccaca ggagcacggc ggccccgatc atgtcgtaca aggccacatg gtgatagatg
     5941 ccacagaaac cgttggcacc aaccgccgct tcggtgcatt ccagaccgtc gtgctggggt
     6001 gcgacgtcat agccgggacg aatcccgtat ccccaccagg cggtagtggc cctccctagg
     6061 tgctcgacga tggcgaggtc accgattctc cccacgacgg tgcccaaagc aagcccgaac
     6121 gccgacatgt ccaatgtcgc ccaaaggggc atcttcaacc tgtgggctcg ccagccaccg
     6181 gcgagaatgc caccgaggaa cccacccatg atcgagaagt ttcccgcgat cgggttgagc
     6241 gcctcgagca gcggcgttcc gtccatgatc tgagccgggg ctgtgaaata gcgggcaccg
     6301 agtagtgccc cgatcaggcc ccaggtgaga caggactggt acttgaccgt gtcgaatccg
     6361 cgcttcgcca tctcccgggt ggcgatccac gccccggcga cgaatcccag ggcagcgaat
     6421 aaaccgtgga gcgagaagct gatcggtccc agatcccaga tcggaatcgg cgggtaggtg
     6481 aatgcggcga gcatcggcgt gagggtacct atcgccgggg ccaagctgac agccacagtg
     6541 cggcgcgagg ttgggcctcg gccgaagtcg gccggcatgt tcgaccggca ttagtgtccc
     6601 cacccgtgag cgacctgctg ccgactgtgg ctgccgccct gaacacaccc gaggccctca
     6661 tcaaacgatc ggccgcggcc cgggctgctg ccaatggcac cactatcgac gaggtgctcg
     6721 ccgcatgggc cggaggtgcc ccggtcgtag cggccgctcc cgcccaggct cccgaaccag
     6781 ccgtggctca gacaacaacc gatcccgacc ctgtgcccga accggttgct actccgacca
     6841 ccatcacccc ggcacccgtt gcggtcatcg ttgaaccgga gccggagcca tcagtccctc
     6901 tcgagccggt tcctatcagc aagcgcatca gcagcgccac cagggtcggc gcttggaccg
     6961 gggccgttct cggtctcacc gggtttctgg tcgcaaccac gtggtgggct caaaacgcaa
     7021 ccgtgatcgg agaaggcccg ttcactcctg tgattcagac gacatcgcgc acggtcatca
     7081 tcggtgccgc cctggtcagc atcctcttcg gcgccatcac ggccggcctc tcccgcgctt
     7141 cggcctcctg ggccaatccc gggatgcaac tcaggaactc cacctcgtcg actggttggc
     7201 tgggggcctt gatcgggctc gtcctcggca tcggagccgc tgctctcctc accggagggt
     7261 tcgggactcc agtggagggt gccgaaggca tggtgcagct gcccgttctt cccaccttgg
     7321 cagtgatgct ccttggtgga gccgcgctcg gcgcgatcac ggccgccatc acccaggcgg
     7381 tcgcggtacc ggtcaccgtc gatgcaggcg aggtggccga cgtccgaagc cggcttggtg
     7441 gagctttgac catccctgcc attgcgctca tcctgctcgt cattttggtg ctcccattcg
     7501 cctggggcct gatccagtca gcccacatca ccaaggaagc ggcggccctc gtcgcgatca
     7561 tcaccgcgat cggcatcctg ggcttcgcct cgctcgccgg aacccgaccc aatgtcaaga
     7621 tcagcttcgg tgaggcgatg atcgccctga tagggatcgt caccgtcctg ataatcgtgt
     7681 tcgccgtcct gtttgcccgt agtcccggcg aagagccaga agaggcaggc caggaagcag
     7741 cggccgtgtc catcctggcc tagctgccgt ggggccccac tggggtctag gtcacggagc
     7801 cggagatatc gcccagtgcc agggcctctc tcaccgactc ggcaatttcc ctggccacga
     7861 gctcctgagc ttcgacggta gaagccccca gatgcggagt caggaccacg ttgggcagat
     7921 cgagcagcgg gctctccggt gatagcggct cggattcgaa gacgtcgagc gcagccccgc
     7981 ccaggtgccc gtcgaccagg gcccgggcta gcgcctcctc gtcaatcact cctcctcgag
     8041 agacgttgac cacgaacgcc ccgggcttca tcttcgagat ggcgaccgtg tcgatcagat
     8101 gacgggtctc gtcgttgagg gggacgtgga gggagatgat gtccccttgt ctcagcacct
     8161 catcgagatc tgtgagagca aggccgagat cgtcggccct ctcagcagtg aggtaggggt
     8221 cgtagacgat caccttcatc ccgaaggcac ggcatcggac tgcaacctcg ccgccgattc
     8281 gccccgcccc caccagaccc aaggtccgat ccttgagctg gacacctctc aactttgctc
     8341 gttcccattt cccggcccgc atggaacggt ccgcctcggc cacccgccgc accaccgaaa
     8401 ggatcaacgc cactgtgagt tcggccgcgg caaccgtgtt gccggcagga gagttgagaa
     8461 ccgcgattcc ccgctcggtg gcggcgttga ggtcgatgtt gtccacccca actccggctc
     8521 ggcctacaac cttgagccgc gggcccccgg aaagcatctc ccgggtcacc ttcgtcgcac
     8581 tcctcaccac caggccatcg gcacctgcga gagccgcccg aaactcatcg gtggacgagt
     8641 cgtcgatccg gattacctcg aaccgggcgt cgtccaacaa aggggcgagg cccgaatcgg
     8701 agacgtggtc ggtgaccacg accctgaatt ggcctttcat cgaatcacgt cccccagatc
     8761 ttggaggagg aggtccaact catcgaccgt ccggtcaccc atgtggccga ttcggatggt
     8821 ggtttccttg agcttcccgt agccggatcc gatcacccag ttcctgtcct tcatgccctt
     8881 ggctacctca ggcccggtga tccgctcggg gaggctcaca caggtgatgg tcggcgagcg
     8941 gtagccctct ggagcgaatg gcgccaaccc caggccagac atctcatcga cccactccag
     9001 cgcgcgctcc cgcatcgcgg tgtggcgctt ccaacgggct tcgatgccct cggccaggat
     9061 cctctccaat tgcagccgga gcgcatacat cagcgagata gccggggtcg ccggggtctg
     9121 attgacctcg agactctgag tcagtgcgac cagatcgaag tagacgccct tgtcggcaag
     9181 agtcgcagat cgcttcagca acgcctcgga tgcgacccca aacgccagac cagggggcaa
     9241 ggccattgcc ttctgtgagc cggtcaacac ccagtcgagg ctccaatcgt cgctgcggac
     9301 ctcggcgccg cacacgccgg tgaccgagtc caccaggaaa agggtgtcat cctcccgggc
     9361 gacgacgccg gccaatgctt cgagatcttg gaggacaccg gtggaggtct cggaatgggt
     9421 gacggtgact gcgtcgtagt cccccgacgc caggcgcccc gcgagctcac caggttcgtg
     9481 gtactcaccc cagggcacct cccagatgtc gacatcccgc ccacaagccg ttgcgatgtc
     9541 ggcaaatcgc ttggaaaaag ccccgttgac cagggagagc accttac
//
LOCUS       DBNHXU010000243         7060 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3449845, whole genome shotgun sequence.
ACCESSION   DBNHXU010000243 DBNHXU010000000
VERSION     DBNHXU010000243.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7060)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7060)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7060
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3449845"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2119
                     /locus_tag="AC161X_06080"
     CDS             <1..2119
                     /locus_tag="AC161X_06080"
                     /inference="COORDINATES: protein motif:HMM:NF012602.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR4989155.1"
                     /translation="ESWLRDGPGSNPEGRGQEPFVEVEWDVALDLLAAELDRVRTQFG
                     NQAIFGGSYGWASAGRFHHAQSQIHRFLNSIGGYTSSVDDYSIAAGHVITPHIFGFSF
                     DDFMDEQPHFVEISENTELVVAFGGMPIKNSQVSYGGQGRHMLSSRLETCRDRGVRFV
                     NLSPLGEDLIEAVEAEWIPMRPSTDTAVLLGLAHSLLTAGDHDEGFLDTYCVGWDRLR
                     AYLLGETDGIAKTAEWASAISAVDPDRLRTLAADMARHRTMVTVSWSVQRNDHGEQAY
                     WMAAALAAMLGQIGLPGGGIGYGYGAVGLNGHPVGYHGLPRLPQGKNHVADFIPVARI
                     ADLLLHGGEEFEYNGGRHIYPETHLVYWAGGNPFHHHQDLNRLAKAWQTPDTIVCHEP
                     FWNALARYSDIVLPATTPLERNDVGGSGNDDFIFWMEKVIDPVGEARNDYEIFSELAR
                     RLGLAERFTEGRTSDEWIAHIYQKFHTNHPEYPSLGALKEAGHFQIPEEWIPPLPSQL
                     VAFRDDPVASALSTPSGRIELYSETIASFGYADCPPHPAWLEPAEWLGNAQRYPLHMI
                     SNQPKTRLHSQWDHGETSMNGKVDGREQIGMTFQDATARGLANGDLVRVFNDRGACLA
                     SVAVIRDLIPGVVQLPTGAWWDPVEPGGMCRAGNPNVLTRDLGTSTLAQGPTAQTCLV
                     EIERFIGEAPPVRGHEHPKFIAG"
     gene            complement(2168..3040)
                     /locus_tag="AC161X_06085"
     CDS             complement(2168..3040)
                     /locus_tag="AC161X_06085"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018523083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR4989156.1"
                     /translation="MSLGVHPVIDSHQHFWDPEVAEYPWMTGPYRSLRRRFGPEDLAP
                     EIKACGVDATIVVQARHDLDETRKLLAYASENPWIVGVVGWVDLAKADVSDTIASLRE
                     SAGGRLLVGIRHQVHDEPDPEWLLRPDVEFGLRAVADADLTYDLLVRSREMPVALEVA
                     RRLPDLRLVIDHLGKPPISTGDLEPWASLIGAFRDLENVTCKISGLVTEASWDNWQQH
                     DLVPYVGIGLEIFGPHRLLFGSDWPVCLLAGTYTEVYEASLTTLREFLGADMGSVLGG
                     CAVRTYFNGVDPTE"
     gene            complement(3040..4092)
                     /locus_tag="AC161X_06090"
     CDS             complement(3040..4092)
                     /locus_tag="AC161X_06090"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011955148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR4989157.1"
                     /translation="MVHPADQELTESGGSVMVTGAMGCVGAWTVRELVLRGLDVVAFD
                     RSTDRRRLSLVMSDEELQGVAFVAGDLVELDSLNDTVKSENVTRIIHLGALQIPFCKA
                     DPPRGAMVNVVGTANVFETARRNAIPHVVYTSSIAVFDMPGGRLDHEAHAVPSSHYGV
                     FKLANEGTARAYWNDSGVASIGIRPMTIYGPGRDQGMTSSPTKAILAAVMGLPYEISF
                     GGSTLFQYAPDVAFALVEAALSPGEGAKIFNLNGTRASMIEFVDAIEQAVDGAEGLIR
                     FRANPLPFPDDIDITGLETIGPPPVTDLAQAMDTSVSMFRALQESGRLNLQDHGLEVV
                     GAVAIDTLPPASTSLA"
     gene            complement(4065..5387)
                     /locus_tag="AC161X_06095"
     CDS             complement(4065..5387)
                     /locus_tag="AC161X_06095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-fuconate dehydratase"
                     /protein_id="HIR4989158.1"
                     /translation="MATTIVDLTTVDIRFPTSRALDGSDAMTEAPDYSAAYVVLRTDS
                     PDGIDGHGMTFTTGRGNELCVAGIEALRPLVVGRTLESLTEDLGAFWYSLVDDGQLRW
                     VGPEKGVIHMATAAVVNAVWDLYAKAEGKPLWKLLVDMTPEELIRCIPFRYISDVLTP
                     EEALEILRNMVPGKKEREERILAEGYPAYTTSIGWLGYPDEKVRDLCRSAVADGWRHF
                     KLKVGRNIEEDVRRSGIVREEIGPDRALMLDANQVWGVDQAIEWMGELARFRPWWIEE
                     PTSPDDILGHARIARAMDEFGIGVATGEHCHNRVMFKQLFQANAIRFCQIDACRLGGV
                     NEALAVMVMAAKFGVAVCPHAGGVGLCEYVQHLSIIDYIAVSGSLDGRVVEYVDHLHE
                     HFVDPAIVRGGHYLAPTAPGTSITMHDSSIDAYAFPFGKEWSTPLIKN"
     gene            complement(5479..6567)
                     /locus_tag="AC161X_06100"
     CDS             complement(5479..6567)
                     /locus_tag="AC161X_06100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017993084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HIR4989159.1"
                     /translation="MPVPARVVTSEVTNNSTQEKTKEIRMKWKRAHRWAAIMVVALLV
                     AACGSGEGSSSTEPASEPTTTGAPGTTAAPATTTAGETYKLGLITKFPVDFFFIIEDA
                     AKAWAADHPDVELITGMGESGSDDEGVIALIESMVAQGVDGIAITPTGPAVTDALQAA
                     VDAGVEIVLIDNDLPDWDGKSAVVATDNFAGGVLAGEWLAENLPAGSTIGVLEGVAGV
                     PALDARVEGMIEGLGDADITIVSQTPTDCDQVKGVAAAEDLLTAYPDVSAIYGACGPP
                     IIGALQAIQNAGIAPDAIVVVGFDASGDEVAAIAAGTQDASVAQFPPKMGELGMATLY
                     QAVQGAEVEVNVDTGTAMVTIDNVADFQ"
     gene            complement(6588..>7060)
                     /locus_tag="AC161X_06105"
     CDS             complement(6588..>7060)
                     /locus_tag="AC161X_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4989160.1"
                     /translation="LSPSANFYAGREMAGPGWLPTGMRFMRRRAMSVATREVLDRLHV
                     GLPDLERSVGLMSGGQRQAVAVARATAFAARLVILDEPTAALGVREARGVLDLINRLR
                     GEGKAVIVISHAMDHVIEVADRAVVMRKGRVVGELIPTRESRQQIVSLIVGGEG"
BASE COUNT         1391 a         2210 c         2086 g         1373 t
ORIGIN      
        1 ggagtcgtgg cttcgggatg gcccgggctc caaccccgag gggcggggtc aggagccatt
       61 tgtcgaggtg gagtgggatg tcgccctcga cctgctcgca gccgaactgg accgagttcg
      121 aacccaattc ggcaaccagg caatattcgg gggcagctat ggatgggcca gcgcgggccg
      181 tttccatcac gcccaaagtc agatccatcg attcttgaat tcgatcggcg gctacacgag
      241 ctcggtcgac gactattcca tcgccgccgg tcatgtgatc accccgcaca ttttcgggtt
      301 ctcgttcgac gatttcatgg atgagcagcc gcactttgtc gagatctccg aaaacaccga
      361 gctggtggtg gcctttggcg gcatgcccat caagaacagt caggtgtctt atggagggca
      421 aggccgacac atgctgtcca gccgtctcga gacctgccgg gatcgtggcg tcagattcgt
      481 gaacctgtcc ccgctgggtg aggacctgat cgaggcggtc gaagcggagt ggataccgat
      541 gcgtcccagc accgacaccg cggtcttgct gggtctagct cactctctgc tgacagcggg
      601 tgaccacgac gagggcttcc tcgacactta ctgtgtcggt tgggaccggc ttcgcgcata
      661 tctgctcgga gaaacggatg gcatagcaaa gaccgcggag tgggcgagcg cgatcagtgc
      721 ggttgacccg gatcgactgc ggaccctcgc cgccgacatg gcccggcatc gaacgatggt
      781 cacagtcagt tggtcggtgc agaggaacga tcacggtgag caggcatatt ggatggcagc
      841 ggctctggca gcgatgctcg gccagatcgg ccttcccgga ggcggcattg gatacggcta
      901 cggtgccgtc gggctcaacg gtcacccggt cggataccac gggcttcccc gtcttcccca
      961 gggaaagaat catgtcgccg acttcattcc ggtggccagg attgccgatc tgctcctcca
     1021 cggcggtgag gaattcgaat acaacggtgg gcgtcacatt tatccggaga cgcacctggt
     1081 ctattgggcc ggcgggaacc ctttccatca tcatcaggat ctgaaccgcc tggccaaggc
     1141 gtggcaaacg cccgacacca ttgtctgcca tgagccgttc tggaacgcgc tggcccgcta
     1201 ttccgacatt gtcctgccgg cgaccacgcc tctggagcgg aacgacgttg gtgggagtgg
     1261 caacgacgac ttcatcttct ggatggagaa ggtgatcgat ccggtcggcg aggcccgaaa
     1321 tgattacgag atcttctctg agcttgcccg gcgccttggt ttggcggaga gattcaccga
     1381 gggccgaacc tcagacgagt ggattgcgca catctaccag aagttccaca ccaaccatcc
     1441 cgagtatccg tcgttgggtg cactcaaaga ggcgggtcac ttccagattc ctgaggagtg
     1501 gattccgcca ctcccttcgc agttggtcgc gttccgcgac gaccctgtgg cttcagccct
     1561 atccacgccc tcgggtcgga tcgagctcta ttcggagacc atcgcgagct tcggctacgc
     1621 cgactgcccg ccccatcccg cctggctgga acctgcggaa tggcttggca atgcgcaacg
     1681 gtatcccctg cacatgatct cgaatcagcc caagacccgt ctccactccc agtgggacca
     1741 tggcgagaca agcatgaacg gcaaggtcga cggcagggag caaatcggca tgacgttcca
     1801 ggacgcaact gctcgtggtc tggccaatgg ggacctggtc cgggttttca acgatcgggg
     1861 agcctgtctg gcgtcggtcg ctgtcatccg agacctgatc ccaggagtgg tccagttgcc
     1921 aaccggggcc tggtgggacc ccgttgagcc cggaggaatg tgtcgggccg ggaatccgaa
     1981 cgtcctgacc cgggaccttg gcacctcgac attggcccaa ggcccaaccg cacagacctg
     2041 tctcgtcgag atcgagagat tcattggtga ggcgcccccg gtgcggggcc acgagcaccc
     2101 caagtttatc gccggttagc ggcgctcaaa ggccaggatc tggatggcct ctaccgatga
     2161 aagttcgtca ctcggtcgga tcgactccat tgaagtacgt gcgaactgca cagccaccta
     2221 agacggatcc catgtcagct ccgagaaatt ctcgcaatgt cgtcaagctc gcctcgtaga
     2281 cctccgtgta ggtgccggcc agcaggcaaa ccggccagtc tgaaccgaac agcaacctat
     2341 gaggaccgaa aatctcgagt ccgataccta catatggcac caaatcgtgc tgctgccagt
     2401 tatcccatga ggcttccgtg accaagccag agatcttgca agtcacgttc tcaagatctc
     2461 ggaatgcgcc gatcaggctg gcccatggct ccaagtcgcc ggtagaaata ggtggtttgc
     2521 cgagatggtc gataacgagc cgaagatcgg ggagccgtcg agccacctcc aaggctacgg
     2581 gcatctccct gctcctgacc aagaggtcgt atgtcagatc agcatcagca acggccctca
     2641 atccgaactc aacgtctggt cgcagaagcc attctgggtc cggctcgtcg tggacctgat
     2701 gccgaatgcc aacgagcagg cgtccgcctg cgctctcccg gagagaggcg attgtgtcgg
     2761 acacatccgc cttcgcgaga tcaacccagc cgaccacgcc aacgatccac gggttttccg
     2821 atgcatatgc gagcagtttc ctggtttcgt ccagatcgtg cctcgcttga accacaattg
     2881 tggcgtccac tccacaagcc ttgatctcgg gcgcgagatc ctccggtccg aaacggcggc
     2941 gcaaagaccg gtaagggccg gtcatccagg gatattcggc gacttcgggg tcccagaagt
     3001 gctggtgtga gtcgattacc ggatgaacac ccaggctcac tatgccaagg aggtcgatgc
     3061 cggcggcaag gtatcgatcg ccacggcgcc aacgacctcc agtccatggt cttggagatt
     3121 gagacggccc gactcttgga gggcccggaa catcgagacg cttgtgtcca ttgcctgggc
     3181 gaggtctgtg accggtggcg ggccgattgt ttccaggccg gtgatgtcga tgtcatcggg
     3241 aaacggcaga gggttggccc gaaaccgaat caaaccctcg gcaccatcga cggcctgttc
     3301 gatggcatcg acgaactcga tcatcgaggc tcgggtgccg ttcagattga agatcttcgc
     3361 tccttcgcca ggagaaagag cggcctcgac cagggcaaac gcaacatcgg gcgcgtattg
     3421 aaatagggtc gatcctccga aggagatctc atatggaagg cccatcaccg cagcaagaat
     3481 tgccttggtc ggagaactgg tcatcccctg atcacggccc ggtccatata tggtcattgg
     3541 gcggatgccg atgctggcca ctcccgaatc gttccagtaa gcgcgtgcgg tgccctcgtt
     3601 ggccagcttg aaaacgccgt agtgacttga cgggacagcg tgggcctcgt ggtcgagacg
     3661 gccgcctggc atgtcgaaca cggcgatcga actggtgtag accacgtgtg gaatggcgtt
     3721 gcggcgcgca gtctcgaaga cattggccgt gcccaccacg ttgaccattg ctccgcgtgg
     3781 cgggtcggcc ttgcaaaacg gaatctgaag ggcaccgaga tgaatgatcc gtgtcacatt
     3841 ctcggacttg acggtgtcat tcagtgaatc gagttcgacg agatcgcctg cgacaaaagc
     3901 gaccccctgg agttcttcgt ccgacatcac caggctcagt cgtcggcgat cggtagatcg
     3961 atcaaatgcc acgacatcca agccgcgcag gacaagttct cgaactgtcc atgctcccac
     4021 gcaccccatg gcgccggtga ccatcaccga ccctccgctc tcggtcaatt cttgatcagc
     4081 ggggtggacc attcttttcc gaacgggaag gcataagcgt cgatcgagga atcatgcatc
     4141 gtgatactgg tccccggcgc tgtaggggcc aaatagtgac cgcctcggac gattgctgga
     4201 tcgacgaaat gttcatgtaa gtgatcgaca tactcgacca cacgaccgtc gagcgagccg
     4261 ctaaccgcta tgtagtcaat gattgaaagg tgttggacat actcgcatag ccctaccccg
     4321 cccgcatgag ggcagaccgc aactccgaac ttggcggcca tcaccatcac agccagggct
     4381 tcgttgaccc caccgagacg acatgcgtcg atctggcaga accggatggc gttcgcctgg
     4441 aacaattgct tgaacatgac gcggttgtgg cagtgctcac cagtcgcgac tccaatgccg
     4501 aactcgtcca tggccctggc gattcgggcg tggccgagga tgtcatcggg gctggtcggc
     4561 tcctcgatcc accacggccg gaagcgagcc agttccccca tccactcgat ggcctgatcg
     4621 accccccaca cctggttggc atccagcatc aatgcccggt caggtccgat ttcttcgcga
     4681 acgatcccag agcgacgcac atcctcttcg atgttccggc caactttcaa cttgaaatgg
     4741 cgccagccgt cggcgaccgc gctgcggcat aggtcccgga ccttctcatc gggatagcca
     4801 agccagccga tcgatgtcgt gtacgccggg tagccctccg ccaggatccg ctcttcacgc
     4861 tccttcttac ctggaaccat gttccgcagg atctccagcg cctcctctgg agtaaggaca
     4921 tcagagatat agcgaaaggg gatgcagcga atgagctctt ccggtgtcat atcaacaagg
     4981 agcttccata gcggcttgcc ctcggccttg gcatagaggt cccaaacggc attgactacc
     5041 gccgccgtcg ccatgtggat cacgcctttc tccgggccaa cccagcgcag ctgaccatcg
     5101 tccaccagcg aataccaaaa ggctccgaga tcctcagtga gcgactcgag agtccggcct
     5161 accaccaaag ggcgcaaggc ttcgatcccg gcgacgcaca attcgttgcc gcggcctgtg
     5221 gtaaaggtca tcccatggcc gtctatgccg tccgggctat cggtgcggag aacgacgtac
     5281 gctgccgagt agtcgggggc ttcggtcatg gcgtcggacc cgtccagggc tcgcgacgtt
     5341 ggaaaccgaa tatctacggt cgtgaggtcg acgatcgttg tagccatcaa tggtccttag
     5401 ataggagcgt gagcatgcgc gaaatgggct tttgggagcc cagcctgccg ggctcccaaa
     5461 gcactctcgg cgaggctcct actggaaatc cgccacgttg tcgatggtca ccattgcggt
     5521 gccggtgtcg acgttgactt caacttcagc gccctgtact gcctgataca aagtcgccat
     5581 tcccagttca cccatctttg gcgggaactg ggcgaccgag gcgtcctggg tgccggcagc
     5641 gatcgcggcg acttcgtccc ccgacgcatc gaaacccacc acgacgattg catccggtgc
     5701 tatccccgcg ttctgaatgg cctgcagggc cccaatgatc ggtggtccgc aggctccgta
     5761 gatggcgctc acgtccggat atgcagtcag taggtcttcg gcagcagcga cgcccttgac
     5821 ctggtcacaa tcggttggtg tctgcgagac gatcgtgatg tcggcatcgc cgagtccctc
     5881 gatcattcct tcgacccggg catcgagcgc cggaacgccg gcgacgcctt cgagcacacc
     5941 gatcgttgag cccgcgggca ggttctcggc gagccattcg cccgccaaca ctcctccggc
     6001 aaagttgtcc gttgccacaa cggccgactt cccatcccaa tcaggcaggt cgttgtcgat
     6061 gagcacgatc tcgacgcctg catccacagc tgcctggagc gcgtcggtga ccgctggacc
     6121 tgtgggagtg atggcgatgc catcgactcc ctgcgccacc atcgactcga tgagggcgat
     6181 cacgccttcg tcgtcggatc ctgactcgcc catgccggtg ataagctcga catcgggatg
     6241 gtccgccgcc caggcttttg ctgcatcttc aatgatgaag aaaaagtcga cggggaactt
     6301 cgtgatcagc ccaagcttgt aggtctcgcc tgcggtggtc gtagctggcg cggcggtggt
     6361 acccggcgcg ccggtggtcg tgggctcgct ggcaggctca gtgctgcttg agccctcgcc
     6421 gctgccgcag gcggctacaa gcaacgcaac caccatgatt gctgcccatc gatgtgctcg
     6481 cttccatttc atcctgatct cctttgtctt ttcctgggtg gagttgttgg tcacttccga
     6541 tgtcaccacc cgagctggca ccggcatccg ggacaggatt caatccgtca accctcacct
     6601 cccacgatca gcgaaacgat ttgttgacgg gattcacgcg tgggtatcag ctctcctacg
     6661 acccgacctt tgcgcatcac cacagctcgg tcggcgacct cgatcacgtg gtccatcgca
     6721 tgtgaaatga cgatgaccgc ctttccctca ccccgcagcc ggttgattag gtcgaggaca
     6781 cctcgggctt cgcgaacgcc gagtgcggcc gtcggctcgt cgaggatcac aagccgcgcc
     6841 gcgaacgccg tcgcccgcgc cacggccacc gcctgacgct gaccaccgga catcaaaccc
     6901 accgagcgct cgaggtcggg cagaccgaca tgcaatcgat cgagcacctc cctggttgcc
     6961 acagacatgg ctcgccgccg catgaaacgc attccggtcg gaagccatcc cggccctgcc
     7021 atctcccgac ccgcatagaa gttcgccgac gggctgaggt
//
LOCUS       DBNHXU010000244         8879 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1663956, whole genome shotgun sequence.
ACCESSION   DBNHXU010000244 DBNHXU010000000
VERSION     DBNHXU010000244.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8879)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8879)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8879
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1663956"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1688
                     /locus_tag="AC161X_06110"
     CDS             <1..1688
                     /locus_tag="AC161X_06110"
                     /EC_number="2.7.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014066165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008741 - ribulokinase activity [Evidence
                     IEA];
                     GO_process: GO:0019569 - L-arabinose catabolic process to
                     D-xylulose 5-phosphate [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribulokinase"
                     /protein_id="HIR4989161.1"
                     /translation="KGSSAMEAANQVIIGVDYGTASVRAVVVDTGNGRELGEGEYAYP
                     HGTDGVITVPGQAHLARQSAEDYVVGLRVSIQRALVSAEEDGFDRRQVVGIGVDTTGS
                     SPIPVDSKGVPLVLDERFADEPDAMTWLWKDHTSYVETAEINQRVSTDRLPYLGPCGD
                     TYSSEWYWAKLAHFLRSADPAVLEATDGWVELADFVPGYLAGRLDPATMARGICPAGH
                     KAMYDTEWGGLPSTEFLDSIEPGMGAFRYSTVAQPAVAPVGGLTDELAEQLGLQPGIK
                     IAAGAFDAHTGAVGAGVAEGTLVKIIGTSTCDTTVVRRDRPLISGLCGSVPDSIIPGM
                     AGLEAGQSAVGDIFNWFADTLSSGARGTGARLDELANEAAGIPAGASGLLGLDWHNGN
                     RSVLVDPLLSGAMVGMTLATTPAEFYRALIESTAFGAQRIIEQFEANGVPIQRIVLSG
                     GSAKKSELLVQIYADVTGRSVELAGTSQTCAVGSAILASVAAGIYETTEEAQAAMVPK
                     PERVVDPNPRAVGVYAELYEMYRKLHDAFGQRGPSDLYDVMKDLHRIRSEVTS"
     gene            1685..2389
                     /gene="araD"
                     /locus_tag="AC161X_06115"
     CDS             1685..2389
                     /gene="araD"
                     /locus_tag="AC161X_06115"
                     /EC_number="5.1.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000286771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase AraD"
                     /protein_id="HIR4989162.1"
                     /translation="MTNTADLREYVLAQNARLPEEGLVSLTWGNVSGRIDELIAIKPS
                     GVPYAGMKPEDIVVLDLNGNIVAGELRPSIDTATHLELYREFPDIGGVVHAHSTYATA
                     FAQARVPLLCLGTTHADHFNGPVPVTRPLSADEVADEYETNTGRVIVEHFRTQGIDPL
                     HVPAVLAAGHAPFVWGTDAAEAVDNIVALEASARMAVLTGLVTGSKPVPLEDWILAKH
                     HERKHGANAYYGQGDQ"
     gene            2386..3888
                     /gene="araA"
                     /locus_tag="AC161X_06120"
     CDS             2386..3888
                     /gene="araA"
                     /locus_tag="AC161X_06120"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011521133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008733 - L-arabinose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0005996 - monosaccharide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HIR4989163.1"
                     /translation="MIDPGGSELWFVTGSQLLYGDETLAMVADNSRQIVAGLNSSPHI
                     PLNIVVKPVMSSGESIAAIVREANADRRCAGLILWMHTFSPARMWIRGLEELNKPYLH
                     LHTQFARDLPWATIDMDYMNAHQAAHGDREFGYIASRLRSRRKIVAGHWSESRVAERI
                     GTWARAAFAVADAADARIARFGDNMRSVAVTEGDKVEFQRVFGYDVEGYGLGELVTRI
                     GQFADAEIDKLCGEYDDAYDVAPELRPGGDRRESLRDAAAIELGLRSLLEEGGFVAFT
                     DTFENLDGLKQLPGIAAQRIMADGYGFGAEGDWKTAAMVRAMKVMAHGLEGGTTFMED
                     YTYHLDPDGSLTLGAHMLEVCPSIADGTPRCEIHPLSIGGKEDPVRLVFDAQAGPSVN
                     ASVVDLGNRFRMTVARVEVVAPPASTPALPVARSLWKPEPDLGSAAEAWILAGGSHHS
                     SHSRALKVEHLEDLALILGVELVTIDAGTDLRELERELFWNDAAHRLMPR"
     gene            4181..5185
                     /locus_tag="AC161X_06125"
     CDS             4181..5185
                     /locus_tag="AC161X_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013770054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4989164.1"
                     /translation="MKSLSNRKSRFSVLLVVLAMAIAACGGDSTDETTGGTEPATGGE
                     LVTVGFAQTGSESGWRSANTESMKAAFSEENGFELVFNAADNDPAAQIAAVRSFINQG
                     VDAIVIAPIVEDGWEDVLQEAKDAGIPVVLEDRTVTAPEDLYATWVGLDFRNEGVMAG
                     EWAAETFGDTPTKMVVLEGTTGSAPANDRAEGFDEAIAGTAIEKIDSQTGEFTRDGGK
                     TVMEGFLQKYGVDGIDLVYAHNDDMALGAIEAIEAAGGVPGEDIQIVSIDAVHDGMQA
                     LIDGKINFIVECNPLLGELAAGLVNDVLAGTDVEKKVYVSDQTFTQEQAAEALPDRLY
                     "
     gene            5328..6824
                     /locus_tag="AC161X_06130"
     CDS             5328..6824
                     /locus_tag="AC161X_06130"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017201750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR4989165.1"
                     /translation="MRGITISFPGVLALDSVDFFLRPGEVHSLMGENGAGKSTLIKAL
                     TGVYPIDSGTIDVSGTSRIFRSTFEAEAAGISTVYQEINLCTNLSVGENVMLGHEPHK
                     GGRIDWKAVHRLASEHLEGLGISIDTRSMLSNHSIAIQQLVAISRAMVLDAKVLILDE
                     PTSSLDRGEVEQLFAVIRDLRDKGAAILFVSHFLDQVYEIADRITVLRNGKLVGEYLV
                     KDLPREDLVTKMIGRVLKEFEAIASTVAREIDRTETPVMKATDVGRRGVLEPADLDVY
                     EGEIVGIAGLLGSGRTELVRLLYGADRADSGHIEVGGAPAKMTSPRQAIDRHIAFSSE
                     DRRAEGIVADLTVAENILLGIQARKGFRKIRREKQESVVSEYLEALDVRPADPDMVAG
                     NLSGGNQQKVLIARWLATAPELLILDEPTRGIDVGTKADLQRKVAELSEQGLSVIFIS
                     SELEEVLRLAQRIVVMRDRKRIGELESSTVKLDGLIDFIANEGKGSAA"
     gene            6821..7864
                     /locus_tag="AC161X_06135"
     CDS             6821..7864
                     /locus_tag="AC161X_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008363489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989166.1"
                     /translation="MRTVIKHRLFWPVAMLILLIVINTVARSQFASITIRDGRLYGAL
                     IDILRQSAPLMLVALGMTIVIATRGIDLSVGAVMAVSGAVALTIIDGSTDPNQLGVVV
                     LAVVVAILVSLLLGVWNGFLVSVLGIQPIIATLVLMLAGRGVALLITEGFITTVNSTP
                     FEYISTGYLLLLPFAFFIWVAAVAAVGLIERRSALGVLTEAVGINPEASRLAGVRSRG
                     IIWSAYAASAILSGVAGLIYASNIKAADANAAGQFIELYAILAVVVGGTSLMGGKFSI
                     AGTVVGVLIIQTLESSILFLGVSPAQSPVFFALVVVLVVIIQSPTLHRYARRLTRSAR
                     QASEHASDLEPTG"
     gene            7861..>8879
                     /locus_tag="AC161X_06140"
     CDS             7861..>8879
                     /locus_tag="AC161X_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013585228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HIR4989167.1"
                     /translation="MTTVEKAAEVRGQASGPYRVFMSRRASLLPTLTAVAILILLFIG
                     AEIFLRGDFITPRNISALLLDNAYLLILAVGMTFVIMTGGIDLSVGSVMAFTGIWLAS
                     LLEAGTSLAIAIPAVMVGGALVGLLIGILVQFFDVQPFIASLAGLFLARGLAYVVSLN
                     SILVENDAVLSLQSTRFQIGDWYITPTGILALVVTLIGTYVMQYTRFGRTTYAIGGNP
                     QASLMGLKVARTTVSVYVISGVCGGLAGLVLTAYSGAGYPRNGIGTELDVIAAVVIGG
                     TVLTGGRGYVLGSMVGVFVYGTIKTIISFMGAEQSWMQIIIGALLLLFIVVQRAIVAR
                     SVRRT"
BASE COUNT         1664 a         2742 c         2850 g         1623 t
ORIGIN      
        1 ggaaaggttc atcggcgatg gaagcagcga accaggtgat catcggtgtc gactatggga
       61 cggcaagtgt aagggccgtg gtcgtcgaca ccgggaatgg tcgggaactg ggcgagggcg
      121 agtacgcata cccacatggc accgatggcg tcatcaccgt tccgggtcag gcgcatctgg
      181 cccgtcagtc ggcggaggac tacgtagtcg ggcttcgtgt ctccattcag cgcgctcttg
      241 tttcggctga ggaagacggc ttcgaccggc gccaggtcgt cggcatcggg gtcgacacca
      301 ctggatcgag cccgatccct gtcgactcca aaggcgtgcc gcttgtcctc gacgagcgct
      361 tcgcagacga acccgacgcc atgacctggt tgtggaaaga ccacacgtcg tatgtcgaga
      421 cggccgagat caaccagcgg gtgtccaccg accggctgcc ctacctgggc ccatgcggcg
      481 atacgtactc gagcgagtgg tactgggcga agcttgccca cttcctgcgt agcgccgatc
      541 ccgcagtgct agaggcgaca gatggatggg tcgagctcgc cgacttcgtt cccggctacc
      601 tggcgggtcg actcgatccg gcgaccatgg cacgcggcat ctgtcccgcc ggccacaagg
      661 cgatgtatga caccgagtgg ggcggtctgc cctcgaccga attcctcgac agcatcgagc
      721 ccggcatggg tgctttccgc tactcgactg tcgcccagcc agcggtcgcg cccgtcgggg
      781 gtttgaccga cgagcttgcc gagcaactcg gcctccaacc cggcatcaag atcgctgccg
      841 gggcatttga cgctcacact ggtgcagtgg gcgccggggt tgccgaaggc acgttggtga
      901 agatcatcgg aaccagcacc tgcgatacga cggtggttcg ccgcgatcgg ccattgatct
      961 ccggtctctg tggaagcgtt cctgattcga taatcccggg gatggccggg ttagaggcag
     1021 gccagtcggc ggttggcgac atcttcaact ggttcgccga cacgctctcc tcaggggcga
     1081 gaggcaccgg ggcacggctg gacgaattgg caaacgaagc cgcagggatc ccggcggggg
     1141 caagcgggct gctagggctc gactggcaca acggcaaccg gtcggtcctc gtcgatccgc
     1201 tgctgtccgg agccatggtc ggaatgacgc tggcgacaac gcccgcggag ttctaccggg
     1261 cactgatcga atcgaccgct ttcggcgctc agcgcatcat cgagcagttc gaggcgaacg
     1321 gggtgccgat ccaacgaatc gtgctcagtg gcggaagcgc caagaagagt gagttgcttg
     1381 tccagatcta tgcagacgta acgggtcgaa gtgtcgaact ggcggggacc tcgcagacct
     1441 gtgccgttgg ctcggcaata ctcgccagcg tcgccgccgg catctacgag acgaccgagg
     1501 aggctcaggc ggcaatggtc cccaaaccgg agagagtcgt tgacccgaac ccgagggcag
     1561 ttggtgtcta cgcggagcta tacgagatgt accgaaagct tcacgacgcg ttcggccagc
     1621 gcggcccgag cgacctgtat gacgtcatga aggatctgca ccggatccga agtgaggtga
     1681 ccagttgacc aataccgccg acctccgcga gtacgtgctt gcacagaacg ctcgactacc
     1741 ggaagaaggt ctggtgtccc tcacctgggg caatgtgagc gggcgcatcg atgagttgat
     1801 cgccatcaag ccgagcgggg ttccctacgc cggcatgaag ccggaggaca tcgtcgttct
     1861 cgacctcaat ggaaacatcg tcgccggcga attgcggcca tcgatcgaca cggcaaccca
     1921 cctcgagctg tatcgggagt ttcccgacat cggcggcgtg gtccacgctc acagcaccta
     1981 cgccaccgcc tttgcccagg cacgggtccc cctcctctgt ctggggacca cgcacgcaga
     2041 tcacttcaac ggcccggtgc cggtcacccg acccctgagc gcagacgagg tcgccgacga
     2101 atacgagacg aacacaggtc gggtgatcgt cgagcacttc cggacccagg ggatcgatcc
     2161 gctgcatgtc ccggcggtgc tggccgcggg ccatgcccca ttcgtgtggg gcactgatgc
     2221 ggccgaggcg gtcgacaaca tcgtcgcact cgaggcgagt gcccgcatgg ccgtgctcac
     2281 cgggttggtg acgggctcga agccggttcc gctcgaagac tggattcttg caaagcacca
     2341 cgaacgcaaa cacggcgcaa acgcgtatta cggacagggg gaccaatgat cgatcccgga
     2401 ggctcagagc tttggttcgt caccggaagc cagcttctct acggagacga aacactcgcc
     2461 atggtggcgg acaactcgcg gcagatcgtg gcgggtctga acagcagccc gcacattccg
     2521 ctcaacatcg tggtcaaacc ggtgatgagc agtggtgagt cgatcgcagc catcgtccgc
     2581 gaagccaacg ccgataggcg gtgtgccggg ctgatcctgt ggatgcacac tttctcaccg
     2641 gcacgaatgt ggatccgtgg gctcgaggag ctcaacaagc catatctgca tctccacact
     2701 caattcgcac gcgatcttcc gtgggcgacc atcgacatgg actacatgaa cgcccatcaa
     2761 gcggcccacg gcgaccgcga gttcggctac atcgcgagtc gtctccgctc ccgccgcaag
     2821 atcgtcgccg gtcactggtc cgagtctcgt gtcgccgaga gaatcgggac atgggccagg
     2881 gccgccttcg ctgtcgccga cgccgctgat gcccggatcg cccgcttcgg ggacaacatg
     2941 cggagtgtcg cggtgaccga gggcgacaag gtcgagtttc agcgtgtctt cggttatgac
     3001 gtcgagggct acgggcttgg ggaattggtg acccgcatcg ggcaattcgc tgacgccgag
     3061 atcgacaagc tgtgcggcga gtacgacgac gcctatgacg tggcccccga gcttcggccc
     3121 ggcggtgacc gccgtgagtc gctgcgggac gctgcggcga tcgagctcgg ccttcggtcg
     3181 cttctggagg aaggtggctt cgttgcattc accgacacct tcgagaacct cgatggcctc
     3241 aagcaactgc ccggcatcgc cgcgcagagg atcatggccg acggctacgg gttcggggcg
     3301 gaaggcgact ggaagacggc ggcgatggtc cgggccatga aggtcatggc gcacgggctc
     3361 gagggcggga ccaccttcat ggaggactac acctaccacc tcgaccctga tggatcgctc
     3421 acactcggtg cgcacatgct cgaggtgtgt ccgtcgatcg cggacggcac gcctcgttgt
     3481 gagatccacc ccctgtcgat tggtggcaag gaggacccgg tccgactcgt tttcgatgct
     3541 caggcgggac ccagtgtcaa cgcgtcggtc gtcgacctgg gcaacaggtt ccggatgaca
     3601 gtggcccgcg tcgaggtagt tgcccctccg gcgagtaccc ccgcactccc ggtggcgcga
     3661 tcgctctgga agcccgagcc cgatctgggc agtgctgccg aggcttggat cctcgccggg
     3721 ggctcacacc attcgagcca cagcagggcg ctgaaggtcg agcatctcga ggacctggca
     3781 ctgatcctcg gtgtcgagtt ggtgacgatc gatgccggga ccgatctcag agagctcgag
     3841 agggagttgt tctggaatga cgcggcacac cgtctgatgc ctcgatgaag gtgttgctca
     3901 ggcggtagtc ccgtcacgag aaagcagatg catgcaagtg agtagacagg gaacaaccaa
     3961 gatgttagcg ttcccgttaa cgataacaac ccaaccaaat caccccaaag tcacccccaa
     4021 tggatgagaa gagaaggaga gcccccaacg accagatacc tgaggagcaa ggccgtgacc
     4081 tacaacggca ccggatggag acaaacatcc gacgggtgcg aggaatccct gagcatcacg
     4141 tcgacgaaag agaatgaacc ccaaacagtg aggaggagct atgaaatcac tgagtaaccg
     4201 gaagtcgcga ttctcggttc tgctggtggt gctcgcgatg gctattgccg cttgcggtgg
     4261 cgacagcacg gacgagacga caggcggaac ggagccggct accggcggcg agctcgtcac
     4321 agtcggcttc gcgcagaccg gatccgagtc gggctggcgt tcggccaaca ccgagtcgat
     4381 gaaagcggct ttctcggagg agaacggctt cgagctggtc ttcaacgcgg ccgacaacga
     4441 cccggcggca cagatcgccg cggttcgcag cttcatcaac cagggtgttg acgcgatcgt
     4501 catcgccccg atcgttgaag acggctggga agacgtgctg caggaagcca aggacgctgg
     4561 gattcccgtc gttctggaag accgcaccgt cactgctccg gaagacttgt acgccacttg
     4621 ggtgggtctt gacttccgca acgagggcgt gatggctggt gagtgggccg ccgagacgtt
     4681 cggcgacacc ccgaccaaga tggtggtcct cgagggcacg accggatctg ctcccgccaa
     4741 cgaccgtgcc gagggcttcg acgaggcaat cgccggcacc gcgatcgaga agatcgactc
     4801 gcagaccggt gaattcactc gcgacggcgg caagaccgtg atggagggct tcctccagaa
     4861 gtacggagtg gacggcatcg acttggtcta cgcccacaac gatgacatgg cccttggtgc
     4921 catcgaggcc atcgaggcag cgggcggcgt accaggcgag gacatccaga tcgtctcgat
     4981 cgacgcagtg catgacggca tgcaggcgct gatcgatggc aagatcaact tcatcgtcga
     5041 gtgcaaccca ctcctcggtg aactggccgc tggtctcgtg aatgacgtgc tcgccgggac
     5101 cgacgttgaa aagaaggtct acgtcagcga ccagaccttc acccaggagc aagccgcaga
     5161 ggccctcccc gatcgcttgt attgatctgc actgactgag accaagtaga taggttgcag
     5221 tgctctaccg gggcgccggt tcgccggcgc cccggtcggc actccataca gatcgagggt
     5281 gaactgtggc cttgtccgcg gatacaagcg accgtcgtat cgtcgagatg cgaggaatca
     5341 ctatcagctt tccgggtgtg cttgccctcg acagcgtcga cttctttctt cgcccgggcg
     5401 aggtgcactc tctcatgggc gagaacggcg ccgggaagtc caccctcatc aaggcattga
     5461 ccggcgtcta tcccatcgac agcgggacga tcgacgtgag cggcaccagc cggatcttcc
     5521 gcagcacgtt cgaggccgag gcagcaggga tctcgaccgt gtaccaggag atcaacctct
     5581 gcacgaacct gtccgtcggt gagaacgtca tgctcggcca cgagccgcac aaggggggcc
     5641 ggatcgactg gaaggccgtg caccgattgg cttccgagca cctcgaaggc ctcgggatct
     5701 cgatcgacac gcggtcaatg ctctccaacc actcgatcgc gattcagcag ctggtggcaa
     5761 tcagccgggc gatggtgctg gacgccaaag tcctcatcct cgacgagccg acgtcgagcc
     5821 tggaccgagg cgaagtggag caactgttcg cggtgatccg cgatctgcgg gacaagggag
     5881 ctgccatcct cttcgtgagc cacttcctcg accaggtata cgagatcgcc gaccgcatca
     5941 cggtcctacg caacggcaag ctcgtcggcg agtacctcgt taaggacctg ccccgtgaag
     6001 atctcgtcac caagatgatc ggtcgggtgc tcaaggagtt cgaggccatc gcctcgaccg
     6061 tcgcgcgaga gatcgatcga actgagacgc cagtcatgaa ggccaccgac gttggcaggc
     6121 gcggtgtgct ggagcccgct gatctcgacg tgtacgaggg cgagatcgtg gggatcgccg
     6181 gcctgctggg ctccgggcgc accgagctcg tgcgattgct gtacggagcc gaccgcgccg
     6241 actccggcca tatcgaggtg ggaggtgcgc cggcgaaaat gacctcacct cgccaagcga
     6301 tcgaccgcca catcgccttt tcgtccgagg atcggcgcgc cgagggcatc gtggccgacc
     6361 tcacggtcgc agagaacatc ctcctcggca tacaggcgcg gaagggcttc cggaagattc
     6421 gcagggagaa gcaagagtcg gtggtgtccg agtacctcga ggcgctcgac gtgcgaccgg
     6481 ccgacccgga catggtcgcc gggaacctgt cgggaggcaa tcagcagaag gtgctcatcg
     6541 cacgttggct cgccaccgca cccgagctcc tgatcctcga cgagccgact cgaggcatcg
     6601 atgtcggcac caaggccgac cttcagcgca aagtcgcaga gttgtcggag cagggcctgt
     6661 ccgtgatctt catttcgtcg gagctcgagg aggtgctgcg actcgcccag cgaatcgtcg
     6721 tcatgagaga ccgcaagcgc attggcgagc tcgaatcatc gaccgtgaag ctggacggcc
     6781 tcatcgactt catagccaat gaaggcaagg ggagcgcagc ttgaggaccg tgatcaagca
     6841 ccgactcttc tggccggttg ccatgttgat cttgctcatc gtcatcaaca cagtcgcacg
     6901 ttcgcagttt gccagcatca ccatccggga tggccgtctc tacggcgcct tgatcgacat
     6961 cctccgtcaa agcgctccat tgatgctggt cgcattgggc atgaccatcg tcatcgccac
     7021 gcgcggaatc gatctgtcgg tcggagcggt catggccgtg tccggagcgg tggctctcac
     7081 catcatcgac ggctcgaccg atccgaacca acttggcgtg gtcgtgctgg cggtggtggt
     7141 cgcgatcctg gtctcgctcc tgctcggtgt atggaatgga tttctcgtct cggttctcgg
     7201 catccagccg atcatcgcaa cgctggtgct catgctcgcc ggccgcggtg tggcactcct
     7261 tatcaccgag ggcttcatca caaccgtgaa cagcacgccg ttcgagtaca tatccaccgg
     7321 atacttgttg ctgctgccat tcgccttctt catttgggtc gcggcggtcg cggcggtggg
     7381 gttgatcgag cgtcgcagcg cactcggcgt ccttaccgag gcagttggca tcaaccccga
     7441 agccagccgt ctggccgggg tgcgctcgcg cggcattatc tggagcgcct atgccgccag
     7501 cgccatcctt tcgggcgtcg cgggcctcat ctacgcctcg aacatcaagg ctgccgacgc
     7561 caacgcggcg ggccaattca tcgagctcta tgcgatcctc gcggtggttg taggcggtac
     7621 ctcgctgatg ggtggaaaat tcagcatcgc gggcacagtc gtgggcgtgt tgatcatcca
     7681 gaccctcgag tcctccatcc tcttcctcgg ggtgtcgccg gcgcagagcc cggtgttctt
     7741 cgccttggtg gtggtgctgg tggtgatcat ccaatcgccc acattgcatc gatatgcgcg
     7801 gaggctgact cggtcggcgc gacaggccag cgagcacgcc tctgacctgg agccgaccgg
     7861 atgacgacgg tcgagaaggc agcggaggtg cgaggacaag ccagcgggcc ataccgagtt
     7921 ttcatgagca ggcgggcgtc tctccttccc acgctgaccg cggtcgccat cctcatcctg
     7981 ctcttcatcg gcgccgagat cttcctgcgc ggggatttca tcacccctag gaacatctcc
     8041 gcgcttctct tggacaacgc ctacctgctg atcctcgcag tggggatgac cttcgtcatc
     8101 atgaccggag gcatcgacct gtcggtgggc tcggttatgg ccttcaccgg gatctggctc
     8161 gcaagtctgc tggaggccgg gacgtcgttg gcgatcgcca tcccagccgt catggtgggt
     8221 ggcgcactgg tgggcttgct catcggtatc ctggtgcagt tcttcgacgt ccagcctttc
     8281 atcgcgtcgc tggctggcct gttcctcgct cgcggcctcg cttacgtcgt gagcctcaac
     8341 tcgatcctgg tcgagaacga cgccgtgctc tcgttgcagt cgaccaggtt ccagatagga
     8401 gattggtaca tcaccccgac aggcatcttg gcgctggtgg tcaccctgat cgggacctac
     8461 gtcatgcagt acacccgatt cgggcgcact acctacgcca tcgggggcaa cccccaagca
     8521 tcgttgatgg gtctcaaggt ggctcggacc actgtgtcgg tgtacgtgat ctcgggggtg
     8581 tgcggcggtc tcgccggtct cgtcctcacc gcctattcgg gcgccggcta cccacgtaat
     8641 ggcataggca ccgagctcga tgtgatcgcc gcagtggtca tcgggggcac ggtgctaacg
     8701 ggaggccgcg gttacgtatt gggctcgatg gtcggcgtgt tcgtctacgg caccatcaag
     8761 accatcatct cgttcatggg tgccgagcag tcctggatgc agatcatcat cggcgcactg
     8821 ctcctgctgt tcatcgtggt ccagcgtgcg atcgttgccc ggtcggtccg gcgaactta
//
LOCUS       DBNHXU010000245         1776 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4840129, whole genome shotgun sequence.
ACCESSION   DBNHXU010000245 DBNHXU010000000
VERSION     DBNHXU010000245.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1776
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4840129"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..844)
                     /locus_tag="AC161X_06145"
     CDS             complement(<1..844)
                     /locus_tag="AC161X_06145"
                     /inference="COORDINATES: protein motif:HMM:NF017321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989168.1"
                     /translation="MTDLFSERANDLAGRNAPLATRMRPRSLDEVVGQTALMGPDSAF
                     RRVVESGRPISMLLWGPPGTGKTTLAWVVANHADAAFETLSATSAGVKDIREVLAGAR
                     ARLETDERRTVLFLDEIHRFAKNQQDALLPGVEDGTLTLIGATTENPFFEVNSPLMSR
                     MTLFRLESLGPTDVEQLVTRALGDEERGLGDRHLTIAADALEELGNRTGGDARQALNA
                     LEVAALLASGASHDEISIDDVGEALQRRIVRYDKTGDRHYDVISAFIKSVRGSDPDAA
                     LYWLF"
     gene            complement(875..1357)
                     /locus_tag="AC161X_06150"
     CDS             complement(875..1357)
                     /locus_tag="AC161X_06150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989169.1"
                     /translation="MTLTATRVVPTHRPLSVFTSFWLLMVLGISALAGGIALTFGSAG
                     SGMAPPADWLDAIPVVESWVIPGLVLGIGFGLGSLVTAYGVWRHPHWGWVSSVETLTK
                     HHWPWIATILIGLGQVIWIGLELLFFPELSVLQAFYGPVGLALLLLPFTRSMSEHLKA
                     "
     gene            complement(1441..>1776)
                     /locus_tag="AC161X_06155"
     CDS             complement(1441..>1776)
                     /locus_tag="AC161X_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HIR4989170.1"
                     /translation="DPVVQRQVFEILGIDSETADKRFGWFLEALRYGTPPHAGFAFGI
                     DRLVMVLQGESSIREVIPFPKTQTGMDPMTGAPTWVDDAQLGELGIAITPEARARQSE
                     PDPSPAEPA"
BASE COUNT          351 a          571 c          556 g          298 t
ORIGIN      
        1 ggaacaacca atagagagca gcgtcgggat ccgagccgcg aacactcttg atgaaggcgg
       61 agatcacgtc gtaatgacgg tccccggtct tgtcatagcg aacgatcctg cgttgcagcg
      121 cctccccgac gtcgtcgatc gagatctcgt catgcgacgc tccgctggcg aggagcgcag
      181 ccacctcgag ggcgttcagg gcctgcctgg catctccccc ggtccggttg ccaagttcct
      241 cgagggcgtc agcggcgatc gtcaggtggc gatcacccaa gcctcgctcc tcgtcgccga
      301 gtgcccgggt cacgagttgc tccacgtcgg tggggccgag cgactcgaga cggaacaggg
      361 tcatcctgct catgaggggt gaattgactt caaagaatgg gttctcggtg gtggccccga
      421 tcagcgtcag cgtcccgtct tcgacaccag ggagcaaggc gtcctgttgg ttcttggcga
      481 accgatggat ctcgtcgaga aagaggaccg tgcgacgctc gtcggtttcc agcctcgccc
      541 tggctcccgc gagcacctcg cggatgtcct tgacccctgc ggatgtcgcc gacagtgtct
      601 cgaaggcagc gtcagcgtgg ttggccacca cccatgcaag cgtggtctta ccggtgccgg
      661 gcggacccca gagcagcatc gagatcggcc gccccgattc gaccaccctg cgaaaggcgg
      721 agtcagggcc catcaaggcc gtctggccca ccacctcgtc caacgaacgg ggacgcattc
      781 gggttgcgag cggggcgttt cgaccggcca ggtcgttggc ccgctcggag aacaggtcag
      841 tcatccgtcc gacggtaacg ggttgtacag attcctatgc cttcaggtgc tcactcatcg
      901 accgcgtaaa cggcaggagc agcaacgcca gcccgaccgg gccatagaag gcctgaagca
      961 ccgacaactc gggaaagaac agcaactcga gcccgatcca aatgacctgg cccagcccga
     1021 tgaggatcgt ggcgatccaa ggccaatggt gcttcgtgag ggtctcgaca gaagacaccc
     1081 agccccaatg cggatgacgc caaacaccgt aggccgtcac cagcgacccg agcccgaagc
     1141 cgatgccaag caccaggccc gggatcaccc aactctcgac cacagggatg gcatcgagcc
     1201 aatccgcggg aggtgccatt ccgcttccgg cgctgccgaa tgtgagggcg atcccgccgg
     1261 cgagagcgct gatcccaagg accatgagca gccagaacga ggtgaaaacc gagagcggtc
     1321 gatgagtcgg gactacccga gtagctgtga gcgtcacaaa cgcctccttt cacgtttgtc
     1381 tcatcatccg acagaggctc acagctctgc caggggccaa cgtcacatca gaggctgacc
     1441 tcaggcgggc tcggccggtg acgggtcggg ctctgactgc cttgctcgcg cctccggggt
     1501 gatcgcaatt cccagctccc ccaactgggc gtcatccacc caggtcgggg ccccggtcat
     1561 cgggtccatc ccggtttggg tcttggggaa ggggatcact tcacggatgg atgactcgcc
     1621 ttggagcacc atcactagac gatcgatgcc aaaggcgaac cctgcgtgtg gaggtgttcc
     1681 gtagcggagg gcctcgagga accaaccgaa tcgcttatcc gcggtttcag agtcgatgcc
     1741 gaggatctcg aagacctgac gttgcaccac cgggtc
//
LOCUS       DBNHXU010000246         5325 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3053442, whole genome shotgun sequence.
ACCESSION   DBNHXU010000246 DBNHXU010000000
VERSION     DBNHXU010000246.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5325)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5325)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5325
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3053442"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1318)
                     /locus_tag="AC161X_06160"
     CDS             complement(<1..1318)
                     /locus_tag="AC161X_06160"
                     /inference="COORDINATES: protein motif:HMM:NF006056.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR4989171.1"
                     /translation="MQRLILKGGHIISLDSSIGEIEVGDVLIEDNRIVKVAPDIDAAD
                     SEVVDATDMLLMPGLVDTHRHTWQTAIKGMSSDWSLPEYLAGIRSRIGPRFTPEDVYA
                     ATLLGATEALNSGVTTLVDWAHIMNSPGHADGSIAALREYGGRAMFAYGTPNDENIAE
                     WYIDSTIPHSDDIRRVRNEELSDDDGLVGLMMAARGPQHTTPETSAGDWRLARELGIR
                     ITAHAGEGAWSLRHQPVLRLHEQGVLGEDTTLVHGNQFSDEDLQLMADSGASISISPE
                     VEMHMGLGFPVTGRALAHGIVSSLSVDVPVTVGGDMFSVMRCALATERALVNEESIRR
                     VEMVERLTISSEDVLRMATIDGARSCGLGDRVGTISPGKEAEIVMIRANDLNLAPLNR
                     PRAAVVVMAHAGNGDSVLVGGRFVKRSGQMLSVDVPSVLERATASRD"
     gene            1508..1954
                     /locus_tag="AC161X_06165"
     CDS             1508..1954
                     /locus_tag="AC161X_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007457504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron chaperone"
                     /protein_id="HIR4989172.1"
                     /translation="MTTTKKTGSDGFSKEERAAMRARAQELKAQGRSGLKQADNEQAV
                     LDAIAAMPIADRVLAERIHALVNANAPQLLSRTWYGFPAYANPDGKLICFFKAASKFD
                     GRYAMLGFEDAAALDDGPMWPVVFAIVEWTPAVEKKLAALIKSAVS"
     gene            2015..2401
                     /locus_tag="AC161X_06170"
     CDS             2015..2401
                     /locus_tag="AC161X_06170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012230543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR4989173.1"
                     /translation="MPVARSRTARAARRRKRRMDRVEHDLSAAQWTALKAAWSGCAYC
                     GASDKPLQRDCVLAISRGGRYTLDNVVPACRSCNASKGSQEVTTWMRSRRRDEGSFLL
                     RHFEISRALAAQDVDEVDVPTAPGRT"
     gene            2398..3357
                     /locus_tag="AC161X_06175"
     CDS             2398..3357
                     /locus_tag="AC161X_06175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014983491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR4989174.1"
                     /translation="MRAVVYDRYGGPDVLRTEEVPKPSPGPGQVLVEVMATSLNLSDW
                     ECLRGTPFYARMGGLRSPARRTLGSDIAGRVESVGPDVTRFRPGDEVYGDNLALMGGF
                     AEYALAPEKALEHKPPTLRFVAASTIPQAGPIAIQGTAGIGPGQRMLINGAGGGSGSF
                     AIQLAKRAGAAVIAVDNASKLEHMRSMGADEVVDYRSQDWTRMGPYDRVLDLVAQRSV
                     FAFRRALAPSGRYRCVGGSVPALLRVVTAGAILGLLTGRRIGLLVVKLGPTHFRPMAD
                     LCVSGDIQIHIDRTFGLEEVPEALAHVGDGRALGKVVVEVKPE"
     gene            3354..4382
                     /locus_tag="AC161X_06180"
     CDS             3354..4382
                     /locus_tag="AC161X_06180"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014984672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR4989175.1"
                     /translation="MSRRPSERTAGEMPIDRTPAARLVGVRRLEQVEAPVPDPGPGEV
                     LIRVLAMGLCGSDAHWFREGGIGDATIPQGGVIPGHEFCGVIESGPRQGERVAIDPAI
                     PCLECEECARGKLNLCLRMRFAGHGSTDGALRRHLVWPERCLVSLPDSVPDDEGALLE
                     PLGIALHATDLGDVGVSDATGVIGCGPIGLLVISALFAQGVKTILAAELLDHRLAVAR
                     ELGAERWSDDSGLDVVFDCAGTDESLHTALEALAPGGRVVMVGVPDGDRTSFVASLAR
                     RKEASLLTCRRMLPEDLERAAALAGAGEVDLGALITHRFPMSSAHSAFETLVARTGIK
                     VVVLPGHS"
     gene            complement(4373..5305)
                     /locus_tag="AC161X_06185"
     CDS             complement(4373..5305)
                     /locus_tag="AC161X_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HIR4989176.1"
                     /translation="MSFAFKDEVGRSVEYLASHLAQDSIQQDPYWPKWDSPWWHMTLL
                     WEMGLAELIPKSAVDAMVDAFRSRDLTFFPLRFDLLPPEIDTRRHVVCHCAVGTMYQV
                     LSACEIEVDEELPWMRPWFFRYQLPDGGLNCDEEAYTKATPKSSIVSTLPPLEAVLFS
                     TGRDFDFEEVEFLDRGAEYLIDHRLYRSVTSGEVTVPEWLDLCFPRFYMYDVLRGLRF
                     LARWAVVRGRSLPQTLVDEASEEIRKSLTAAGLVPGRRPWIGETTLMLGEDGEWSDKA
                     ESRFSLLEAVSRTDAVSPNLTGLWEAVEADLAQL"
BASE COUNT          957 a         1688 c         1712 g          968 t
ORIGIN      
        1 agtcacgcga ggcggtggcc ctctcgagga cggacggcac gtcaactgac aacatctgcc
       61 ccgagcgttt gacgaagcgc cctccaacca ggaccgagtc cccgttcccg gcgtgggcca
      121 tgaccaccac cgcagccctc ggcctgttga gcggcgccag attcaggtcg ttggcgcgga
      181 tcatgacgat ttccgcttcc ttccctgggg agatggtccc gacccggtcg ccgagcccac
      241 atgatcgggc tccgtcgatg gtcgccatcc gcaggacgtc ctcggaactg atcgtcaacc
      301 gctccaccat ctccacgcgt cggatcgact cctcgttcac cagggctcgc tcggtggcaa
      361 gggcgcagcg catgactgag aacatgtcgc ctccgacggt gactggcaca tccaccgaaa
      421 ggctggacac gatgccatgc gccagtgccc gccctgtcac cgggaagccg agccccatgt
      481 gcatctcgac ctcgggtgag atggaaattg atgctccgct atcggccatc agctgaagat
      541 cctcgtcgga gaactggttg ccgtggacca acgtggtgtc ctcgccgagc acgccttgct
      601 cgtgtagacg gaggacaggt tggtggcgca gggaccaggc gccctcgccg gcgtgggcgg
      661 tgatcctgat cccgagctct cgggcaagtc tccaatcgcc ggcacttgtt tccggcgtgg
      721 tgtgctgcgg gccccgggcc gccatcatca aaccgaccag cccatcgtcg tctgacaact
      781 cctcattgcg caccctgcgg atatcgtcgg aatgcgggat tgtgctgtcg atgtaccact
      841 cggcgatgtt ctcgtcgttt ggtgtcccgt aggcgaacat cgcccgtccc ccatattccc
      901 tgagagcggc aatcgaaccg tcggcgtgcc ccggggagtt catgatgtga gcccagtcga
      961 ccagggtcgt cacgccagag ttcagggctt ccgtggcccc aagcaaagtg gctgcgtaca
     1021 cgtcttccgg tgtgaaccgg ggaccgatcc ggcttcgaat tcccgccaga tactcgggga
     1081 gcgaccaatc ggaggacatg cccttgatcg ccgtctgcca tgtatgccgg tgagtgtcga
     1141 cgagaccggg catgagcaac atgtccgtgg cgtcgaccac ctcgctgtcc gccgcatcga
     1201 tgtccggggc aaccttcacg atcctgttgt cctcgatcaa gacatcgccc acctcgatct
     1261 ctccaatcga agaatcgaga gagataatgt gaccgccttt gagtatcaat cgctgcactt
     1321 gtgtggccct cgtttgcgtc cgcggcactc taaacacaag ggtgacccgt cttggcggtg
     1381 ccgagtcacc agcaatcgcg ctcgattacg catgcgagac cgcagctacc cgattgagca
     1441 ggatcggaga agcgcacttc cgacatcccc cgtagtgtcg gttgcgagtc gtattggagg
     1501 tcaagtgatg acaaccacga agaagaccgg ttcggacggt ttctccaagg aagaacgtgc
     1561 tgccatgagg gcgcgggctc aggagttgaa ggcgcagggc cgcagcggcc tgaagcaagc
     1621 cgacaatgag caggccgtgc tcgacgcgat cgccgccatg ccgattgccg atcgagtgct
     1681 cgccgagcgc atccacgccc ttgtgaatgc caatgcgccg cagctcctgt ccaggacctg
     1741 gtacggcttc ccggcctacg ccaacccgga cggcaagctg atctgcttct tcaaggcggc
     1801 gtcgaagttc gatggccgct atgcgatgct gggattcgaa gacgcggccg ccctcgacga
     1861 cggcccgatg tggccggtcg tgttcgccat cgtcgaatgg actccggcag tcgagaagaa
     1921 gctcgcggcg ctcatcaaat cggcagtcag ctgagcctcg caaccgggcg gtggccggct
     1981 caaaccccgg agatcgacgg aataccctga ggtgatgcca gtcgcgagaa gtcggacggc
     2041 gcgggcagct cggagacgca agcgtcgtat ggaccgggtc gagcacgacc tcagcgccgc
     2101 ccagtggact gccctcaaag cggcctggag tggttgcgcc tactgcggtg cctccgacaa
     2161 gccactgcag cgcgactgtg tgttggcgat ctcccgcggc ggccgctaca cgctcgacaa
     2221 tgtcgttccg gcgtgtcgct cctgtaatgc cagcaagggc agtcaggaag tcaccacctg
     2281 gatgcgatcg agacgacgcg acgagggctc cttcctgctg cgtcacttcg agatctcgag
     2341 ggctctcgcc gcgcaggacg tcgacgaggt ggatgtccca accgctcccg gacggacatg
     2401 agggcagtcg tctacgaccg atacggcggt cctgacgttc tccggaccga ggaggtgcca
     2461 aagcccagtc cgggcccggg tcaggtgctg gtcgaagtga tggcgacgtc gctaaacctc
     2521 tccgattggg agtgtctgcg tggtaccccg ttctatgccc gcatgggagg tttgcggtct
     2581 ccggctcgtc gcacactcgg ctctgacatc gcgggtcgag tggaatccgt cggccccgac
     2641 gtcacccggt ttcgcccagg cgacgaggtc tacggcgaca acctggccct gatgggcggg
     2701 ttcgccgaat acgcgctcgc acccgaaaag gccctggagc acaagccgcc gaccctgaga
     2761 ttcgtcgcgg cctcgaccat tccgcaggca ggaccgattg caattcaagg aactgccggg
     2821 atcggacccg ggcaacggat gctgatcaat ggcgccggcg gcggttcggg atcgttcgcc
     2881 atccagctcg ccaagcgcgc cggtgccgct gtgatcgctg tcgacaacgc gagcaagctg
     2941 gaacacatgc ggtcaatggg cgccgacgag gtggtcgact atcggagcca ggactggacc
     3001 aggatggggc catacgaccg ggttctggac ctcgtggctc agcgctcggt gtttgcgttt
     3061 cggagggcat tggcacccag cggccgttat cgatgcgttg ggggctcggt gccggcgctg
     3121 ctccgggtag tcactgccgg ggcgatcctg ggtctgctca ccgggcggcg catcgggctg
     3181 ctcgtcgtca agctcggccc gacccacttc cggccgatgg ccgacctctg cgtctcgggg
     3241 gacatacaga tccacatcga ccgcaccttc ggtctggagg aggtgcccga ggccctggcc
     3301 cacgtcgggg atggtcgtgc cctgggcaag gtggtggtgg aggtcaagcc agaatgagca
     3361 ggcgcccatc ggagcgcacc gcaggcgaga tgcccatcga ccggacgccg gccgcccggc
     3421 tggtaggcgt gagacgtctc gagcaagtgg aggctcctgt gccggaccca gggccaggcg
     3481 aggttctgat cagggtcctt gccatggggc tatgtggctc cgacgctcac tggttccggg
     3541 agggaggaat aggtgatgcc actattccac agggtggtgt catccccgga cacgagttct
     3601 gcggggtgat cgagtcaggg ccgcgccagg gggagagagt ggcgatcgat ccggccatcc
     3661 catgccttga atgtgaggag tgcgcccgcg gcaagctcaa cctctgtctg cggatgcgtt
     3721 tcgccggaca tgggagcacg gatggcgctc tgcgccgtca cctggtgtgg ccggaacgat
     3781 gcctggtgag cctccccgac tccgttcctg atgacgaagg ggccctcctc gagccgttgg
     3841 ggattgctct tcatgccacc gatctcggtg acgtcggtgt cagtgatgcg accggagtga
     3901 ttggctgcgg gcctattggg ctgttggtga tctcagctct gttcgctcaa ggggtcaaga
     3961 ccatcctggc cgccgagctc ctggaccaca gactggcggt tgctcgtgag ctcggcgcgg
     4021 agcggtggtc ggatgactcc ggcctcgatg tggtcttcga ctgcgccggg accgacgagt
     4081 cgcttcacac ggcgttggag gcgctggcac cgggaggccg tgtggtgatg gttggcgtcc
     4141 cagatgggga ccgtacttcc tttgtggcct cgctcgccag acgcaaggag gcttcgctcc
     4201 tgacatgccg tcggatgctg cccgaggatc tggaacgcgc cgctgcgctg gcaggagcgg
     4261 gagaggtgga tctgggagca ctcattactc accgtttccc catgtcctcg gcccattcag
     4321 cctttgaaac cctggtggcg cggacgggga tcaaggtcgt tgtgttacca ggtcacagtt
     4381 gagccaggtc ggcctccact gcctcccaca gcccggtcag gttcggtgag accgcgtcgg
     4441 ttcggctgac cgcctccaac aatgaaaacc gggactccgc cttgtccgac cattccccgt
     4501 cttcgcccag catcagggtc gtctccccaa tccacgggcg acgtcccgga accagaccag
     4561 cagccgtgag actcttccgg atctcctcgg atgcttcatc gaccagcgtc tgcggaagcg
     4621 accgaccccg gacgaccgcc caacgggcca ggaaccggag accacgcaaa acgtcataca
     4681 tgtagaaccg ggggaaacag agatcgagcc actcggggac tgtgacctca ccactggtga
     4741 ccgaccgata caggcggtga tcgatcaggt attcggcgcc tcgatcaaga aactcaacct
     4801 cctcgaagtc gaagtcacgt ccggtcgaga acagcacggc ttccaacggg ggaagagtgg
     4861 agacgatcga gctcttgggt gttgccttgg tatacgcctc ttcgtcacag ttcagcccgc
     4921 cgtcgggcag ctgatacctg aagaaccagg gccgcatcca gggaagctcc tcatcgactt
     4981 caatctcgca cgcactcaac acctgataca tcgtcccaac cgcgcaatgg cacaccacat
     5041 gcctgcgggt gtcgatctcg ggaggcaaca ggtcaaagcg aagagggaag aaggtcaggt
     5101 cgcgcgatcg gaaagcgtcc accatggcgt cgaccgcact cttcggtatg agctcggcga
     5161 gccccatctc ccagagcagg gtcatgtgcc accacggcga gtcccatttt ggccagtagg
     5221 ggtcttgctg gatgctgtcc tgggccagat gagacgcgag gtactccact gatcggccga
     5281 cttcatcctt gaaggcaaag ctcaagacgc ctcagtctct ccggt
//
LOCUS       DBNHXU010000247         2471 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3648404, whole genome shotgun sequence.
ACCESSION   DBNHXU010000247 DBNHXU010000000
VERSION     DBNHXU010000247.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2471)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2471
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3648404"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1482)
                     /locus_tag="AC161X_06190"
     CDS             complement(139..1482)
                     /locus_tag="AC161X_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HIR4989177.1"
                     /translation="MLTTDEVRSRAKALMPQLTEDLKDLVSHASCSFPGYPVEPVTEM
                     RQAAMELLGRYGADVRELDLGKGKPAIYGEIPGPAGTPTVLLYAHYDVQPAPVEAGWD
                     TDPWTAELRNGRLYGRGAADDKSGVIIHAGTLGVFEGEFPVNLRIVIEGEEETVSHIG
                     PFVDAHPEMFQADTYVIADMGNPAAGVPALTTTLRGNVVCTVKVRSLHHPAHSGIFGG
                     AAPDALLALIHILATLHDDSGDTVVPGLAAFEWSGTEMGEDAFRTAAGMVDGAELIGS
                     GTLGARLWSRPAASVIGIDAPTVKDAANVVLPEAAAKISMRVPPGADSEHELGLLMDH
                     LKRVAPWGVEVEVEKVRVGYPFSAKMDGPAVKAAMAALEAAYETGAGAMGSGGSIPLV
                     ARLQGISPNAEVILWGAEDVAEAKIHASNESVDLTEIERLIVAQALTLQTLGSGG"
     gene            complement(1505..>2471)
                     /locus_tag="AC161X_06195"
     CDS             complement(1505..>2471)
                     /locus_tag="AC161X_06195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005249457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR4989178.1"
                     /translation="AVALFIGEGASATSANLFTAAVIVIVAGIQILGAEAISKIQSLI
                     VVILLAVFAVFIVVTVSEVDAELLSPSLYPPAVDIISSIALTFFAYLGFAVISYAGED
                     LPDPKRDLPKATYIALGITTVLYILISLGVFGTLTVDQVIQYGETALAEAARPVLGDA
                     GFAMMAIAALLATFTSVNANIYGAVGSTSALAKTRQFPPIFGKRGKFGSTVGLTISAV
                     AILLLAVLADLSAIASLGSVVALVIFLMVALAGLRLRAETGSSVLIIWLAIVSTAIVL
                     VVFGIETARNAPETFAAMIIVLVLAAVLEYVWSAMRRSRDAAGPA"
BASE COUNT          479 a          782 c          784 g          426 t
ORIGIN      
        1 attgccctcg ccagcatgtc gctggtggta ctaatcaggg ctgttggcgt ctggcaaggc
       61 tgggtcactc caggttcggt cgacctcacc cctcacagga gatccggcga tcgggtcgac
      121 ccgaacctcc cgagttaatc agccgcccga gcccagggtc tgcaaggtga gagcctgcgc
      181 aacgatgagc cgctcgatct cagtcagatc gacactctcg ttcgaggcat ggatcttggc
      241 ttcggcaacg tcctctgcgc cccacagaat cacctcggcg ttggggctga tgccctggag
      301 ccgggccacc agcggtatcg agccgccgct tcccatcgcc ccggcaccgg tctcataggc
      361 ggcttcgaga gccgccatcg ccgccttgac cgccggtccg tccatcttcg ccgagaatgg
      421 gtagccgacc cgcacctttt ccacctcgac ctcgaccccc catggcgcca cccgcttgag
      481 gtgatccatg agcaggccga gctcgtgctc tgaatcggcg cctggcggga cgcgcatcga
      541 gatcttggcc gcggcttctg gaaggacgac gttggcagcg tccttcactg ttggggcgtc
      601 gatcccgatc accgaggctg cgggtcggga ccagagacga gcgccgaggg ttcctgagcc
      661 gatcagttcg gcaccgtcga ccatgccagc cgccgtccgg aatgcgtcct cacccatctc
      721 ggtgccggac cattcgaacg cggcgaggcc gggaaccacg gtgtctccag aatcgtcgtg
      781 gagcgtcgcc aggatgtgga tgagagcgag cagagcgtcg ggagcggcac ccccaaaaat
      841 gccggagtga gcaggatggt gcagtgacct gactttgacc gtgcagacca cgttgcctcg
      901 cagcgtggtg gtcaaagcgg ggacgccggc tgcggggttg cccatgtcgg cgatgacgta
      961 ggtgtcggcc tggaacatct cgggatgggc gtcgacgaat gggccgatgt ggctgaccgt
     1021 ctcctcctcg ccctcgataa caattcggag attcacaggg aactcccctt cgaagacgcc
     1081 aagggtcccg gcgtggatga tcacaccgct cttgtcgtcg gccgccccac gcccatagag
     1141 ccgcccgttg cggagctcgg ccgtccaagg gtcggtgtcc caacctgcct cgaccggagc
     1201 cggctgcacg tcgtagtggg cgtagaggag cacggttggc gtgccggccg ggccagggat
     1261 ttcgccatag atggcaggct ttcccttgcc cagatccagt tcgcgaacgt cagccccata
     1321 ccggcctaga agctccattg cggcctgccg catctcggtc accggctcca ccggataacc
     1381 ggggaacgaa cacgaggcat ggctcaccag gtccttgaga tcctcggtga gctggggcat
     1441 cagggccttg gcccgtgatc gaacttcgtc tgtggtcagc attcttcatt ccccttggtt
     1501 gtggctacgc aggcccggcc gcgtcgcgac ttcttcgcat agcgctccac acgtactcga
     1561 ggacggcggc caggaccagc acgatgatca tcgcggcgaa tgtctcagga gcattccgcg
     1621 ctgtctcgat tccaaaaaca accaacacga tcgcggtcga aacgatggcc agccagatga
     1681 tcagaacgct ggagccagtt tcggcccgca aacgcagccc ggccagggca accatgagga
     1741 agatgaccaa ggcgaccaca cttcccagcg aggcgattgc gctcagatcg gcgaggaccg
     1801 caaggagcag gatggccaca gccgaaatcg tcagtcccac cgtgcttccg aacttgccgc
     1861 gcttcccgaa gatcggaggg aattgacggg tcttggccag agccgatgtc gagccgaccg
     1921 ctccgtaaat gttggcgttg acggaggtga aggtggccaa caaggcagcg atggccatca
     1981 tggcgaatcc ggcgtccccg agaaccggcc tcgccgcctc ggcgagtgct gtctctccat
     2041 attggatgac ctggtcaact gtgagggtcc cgaagacccc gagcgagatg aggatgtaga
     2101 gaacggtggt tatccctagc gcgatatacg tcgccttcgg aaggtctcgt ttcgggtcag
     2161 gcaggtcctc accggcgtag ctgatcaccg cgaacccgag gtatgcgaag aaggtcagag
     2221 cgatgctcga gatgatgtcc acggccggcg gatacaggga aggggagagc agctcggcgt
     2281 caacctcact cacggtcaca acgatgaaca ccgcgaacac cgccaggaga atgaccacga
     2341 tcaaagactg gatcttggag atcgcctcgg caccaaggat ctggatcccc gcaactatga
     2401 cgatgacggc ggcggtaaag agattggcgg acgtggcgga tgcaccttcg ccgatgaaca
     2461 gggcgaccgc g
//
LOCUS       DBNHXU010000248         2844 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_278027, whole genome shotgun sequence.
ACCESSION   DBNHXU010000248 DBNHXU010000000
VERSION     DBNHXU010000248.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2844)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2844)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2844
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_278027"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..868)
                     /locus_tag="AC161X_06200"
     CDS             complement(<1..868)
                     /locus_tag="AC161X_06200"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR4989179.1"
                     /translation="MSLGFGLLSAQLRPGERDWSRAYEETIGLAVEAERLGLSSIWTT
                     EHHFVDDGYMPSLAVVSAAIAAVTSSIEIGTGVILAPLHHPLRLAEDAATVDLISKGR
                     LILGLGLGWSETEFAGLGADLTKRGAAMEEILTILSEAWSGRVFRHEGQVYDFPEVAV
                     RPTPARPIPVLIGGGAEPAIRRAARLADGIFSNVPTQQFLTQLGWIRDECDRIGRDPG
                     QLRIIHYSVMLPGPSEQDAWDRYADHVWHMSWKYTDMEASTNRPGSPVAAPPMTAEAG
                     ERLRSRSMLAGPP"
     gene            947..1357
                     /locus_tag="AC161X_06205"
     CDS             947..1357
                     /locus_tag="AC161X_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011958375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HIR4989180.1"
                     /translation="MTLADYLPIGLMLLLAGVFVAVSLIVSRFFRPNNPTAEKLDPYE
                     SGIVPEVEPVQRFPVKFYLVAMLFVIFDVEIIFLFAWASIWDQLGWYGVAAIFIFTFF
                     VAETLAYVWKRGALDWNVHRRTRYLPSESTEEAA"
     gene            1358..1816
                     /locus_tag="AC161X_06210"
                     /pseudo
     CDS             1358..1816
                     /locus_tag="AC161X_06210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719105.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B"
     gene            1822..2346
                     /locus_tag="AC161X_06215"
     CDS             1822..2346
                     /locus_tag="AC161X_06215"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HIR4989181.1"
                     /translation="MSDETAIVEEPPARSLPDIPVLHTLFEQFEAVEWESSLGQDVAR
                     VPNEAWLEFATAAKDSGFEVCVDVTAVDWMRDRPDRFEIVANLLSMSEKVRLRMTTTT
                     GRAEPSVASLVPVWPGANFAEREAYDMFGIIFEGHPDLTRILMPDEWEGHPLRKDFGV
                     GSVPVQFKESHQVT"
     gene            2348..>2844
                     /locus_tag="AC161X_06220"
     CDS             2348..>2844
                     /locus_tag="AC161X_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH dehydrogenase subunit D"
                     /protein_id="HIR4989182.1"
                     /translation="MSEPTVEGVESPEITETLEVWVAGTEEPEFSPTEFSPTDEGEQE
                     ISDHESEARLDEQFGRVVEDDYEFDDGVDEGERMILNMGPQHPSTHGVLRLQIELEGE
                     IIRRTKPIIGYLHTGMEKTGEQLNFSQGGTNVTRMDYLAPFHNELAYSLAVERLLGIE
                     VPPRAA"
BASE COUNT          499 a          908 c          917 g          520 t
ORIGIN      
        1 ccggtggacc ggcgagcatg ctccgtgagc gcagtctctc gcctgcctct gctgtcatcg
       61 gcggcgcggc cacgggcgat cccggacggt tggtcgaggc ctccatgtcg gtgtacttcc
      121 agctcatgtg ccacacgtgg tcggcgtacc gatcccacgc atcctgctcg gaaggcccgg
      181 ggagcatgac cgaataatgg atgatcctca gttgtccagg gtcccggccg atccgatcac
      241 actcgtcgcg gatccatccg agctgagtca ggaactgctg ggtggggacg ttggagaaga
      301 tgccgtcggc gagacgagcg gcgcgacgga tcgcaggttc ggcgccacct ccgatgagca
      361 cgggtatcgg acgggcgggg gtggggcgca ccgccacctc tgggaagtcg tacacctggc
      421 cctcgtgtcg gaacacccgc ccactccagg cctctgacag gatggtgagg atctcctcca
      481 tcgccgctcc ccgcttggtc aggtcggcgc cgagcccggc gaactcggtc tcgctccagc
      541 ccagaccgag ccccagtatc agccgtccct tgctgatcag gtcgaccgtg gccgcgtctt
      601 cggccagcct cagcgggtgg tgaagcggcg ccaggatgac gccggtcccg atctcgatgc
      661 tgctggtgac ggcggcgatc gcggcgctga ccacggccag agaaggcata tacccgtcat
      721 cgacgaagtg gtgctcggtc gtccagatcg atgacaggcc gagacgttcg gcctcgacgg
      781 cgagaccgat cgtttcctcg taggccctcg accaatctcg ttcccccggt cgcagttggg
      841 cgctcagcaa tccgaaacca aggctcatcg tggtgctcct gacatgaagt cagcctacga
      901 ccatcgtgat ttaccggacc aaccagcccc gttctaagct cagatcgtga ccctcgccga
      961 ctatttgccg atcggcctga tgctgctcct ggccggagtg ttcgtggcgg tgtcactcat
     1021 cgtttcccgt ttcttccgcc ccaacaaccc gacggcggag aagctggacc cctacgaaag
     1081 cgggatcgtt cccgaggtcg agccggtcca acggttcccg gtgaagttct atctggtggc
     1141 gatgctgttc gtgatcttcg acgtcgaaat catcttcctg ttcgcctggg cctcgatctg
     1201 ggaccagctc ggctggtacg gggtggcggc gatcttcatc tttacgttct tcgtggccga
     1261 gaccctcgcc tatgtctgga agcggggcgc cctcgactgg aacgtccatc gccgcacccg
     1321 ctatctgccc tccgagtcca cggaggaggc ggcctagatg gccacgaccg ctccgggcaa
     1381 cctgatgatg accaccgtcg acaaggcggc gcgttgggct cgcaccagat cgatgttccc
     1441 cgcgaccttt ggcctcgcct gctgtgcgat cgagatgatg gccaccggtg ccgcccacta
     1501 cgacctggcc cgattcggca tggaggtgtt ccgtgcctcg ccgcggcagg ccgacctgat
     1561 gatcgttgcc ggcagggtga gccagaagat ggcgccggtc ttgcgtcagg tctatgacca
     1621 gatgcccgac ccgaagtggg tgatctcgat gggcgcctgc gcctcaaccg gtgggatgtt
     1681 caacaactac gcactggtcc agggcgttga tcaggtggtc ccggtcgaca tctatgtccc
     1741 aggctgccca cccggcccgc agagcctgat gcacgggatc ctgactctcc acgacaagat
     1801 catgaacggc gagatcaaca attgagcgac gagacggcca tcgtcgaaga gccgccggcc
     1861 cgctcccttc cggacatccc ggtgttgcac acgctgttcg agcagttcga ggccgtggag
     1921 tgggagagct ctctcggcca ggatgttgcc cgggtgccca acgaggcgtg gttggaattc
     1981 gccactgccg ccaaggattc gggattcgag gtatgtgtcg acgtgaccgc ggtcgattgg
     2041 atgcgtgacc gcccggaccg gttcgagatc gtggccaatc tcctctcgat gagcgagaag
     2101 gtgcgtctgc gtatgaccac cacgaccggc agggccgaac cttcggttgc ctcgctggtg
     2161 ccggtgtggc ccggcgccaa tttcgccgag cgtgaggcct atgacatgtt cgggatcatc
     2221 ttcgagggcc atcccgatct gacccggatc ctgatgcccg acgaatggga ggggcatccg
     2281 cttcgcaagg acttcggggt gggaagcgtt ccggtgcagt tcaaggaaag ccatcaggtg
     2341 acctgacgtg agcgagccaa ccgtggaagg tgtcgaaagc cctgagatca ctgagaccct
     2401 cgaggtctgg gtcgccggga ccgaggagcc tgaattcagt ccaacagagt tcagccccac
     2461 tgacgaaggc gagcaggaga tctcggacca cgagtcggag gcccggctcg acgagcagtt
     2521 cggaagggtg gtcgaggacg actacgagtt cgacgacggg gtcgacgagg gcgagcggat
     2581 gattctcaac atgggccctc agcacccttc cacccacggc gtactccggc tccagatcga
     2641 gctcgagggc gagatcatcc ggcgaaccaa gccgatcatc ggctacctcc acaccgggat
     2701 ggagaagacg ggggagcagc tcaatttctc ccagggcggc accaacgtga cccgtatgga
     2761 ttatctggcg cccttccaca acgaactggc gtactcgctc gccgtggagc ggctcctcgg
     2821 gatcgaggtc ccgccgaggg cggc
//
LOCUS       DBNHXU010000249         2305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4245435, whole genome shotgun sequence.
ACCESSION   DBNHXU010000249 DBNHXU010000000
VERSION     DBNHXU010000249.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4245435"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..340)
                     /gene="scpB"
                     /locus_tag="AC161X_06225"
     CDS             complement(<1..340)
                     /gene="scpB"
                     /locus_tag="AC161X_06225"
                     /inference="COORDINATES: protein motif:HMM:NF016005.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0051304 - chromosome separation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HIR4989183.1"
                     /translation="MNETAALEAVLFVTETPVPIGELAEVLELPKKRVEELLEELRVD
                     LEARGAGIVLRSVAGGWRLYSHPDAYPYLERFSSSATARRLSPAAIETLSVIAYRQPV
                     ARSQISEIRGV"
     gene            complement(337..1101)
                     /locus_tag="AC161X_06230"
     CDS             complement(337..1101)
                     /locus_tag="AC161X_06230"
                     /inference="COORDINATES: protein motif:HMM:NF014654.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="segregation and condensation protein A"
                     /protein_id="HIR4989184.1"
                     /translation="MSFHVKTTVFEGPLDLLLQLISKHQVEVTAVGLTEVVTEYISFL
                     DEMKGLDLEVTSEFTLIAATLIQLKAQSLLPGRGDFDMDEELALIEERDRLLSRLLAC
                     VTFKDVGVVLLRRLQDANRYVPRTSGVDQRIVTAPADLSLPVDASGLAAAAFRVFKRS
                     DAEPDLDHLDLDLPSVESAIGELRSRVAEMTMASFDDLVEHCERPVEVAAYFLALLEL
                     ARWGLIAITQDDWLADIRVEHREVDDIDLTSEWAAS"
     gene            complement(1113..1628)
                     /locus_tag="AC161X_06235"
     CDS             complement(1113..1628)
                     /locus_tag="AC161X_06235"
                     /inference="COORDINATES: protein motif:HMM:TIGR00277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing metalloprotein"
                     /protein_id="HIR4989185.1"
                     /translation="MPGSLSHLTRRFFDVVLARPLADSEQQAVKDWLTEQENSLFLAQ
                     PAADQRHGYTAAETVVAAGVQDRILIRTALLHDIGKRHARLGAIGRVIASLLILFRIP
                     IRGRWAAYRDHGEVAALELQAIGSDSLIVDFARQHHGERPATIDSDTWELLQKADQPP
                     KTLARNRPGIS"
     gene            complement(1633..1983)
                     /locus_tag="AC161X_06240"
     CDS             complement(1633..1983)
                     /locus_tag="AC161X_06240"
                     /inference="COORDINATES: protein motif:HMM:NF013427.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TraR/DksA family transcriptional regulator"
                     /protein_id="HIR4989186.1"
                     /translation="MSFDIARAKKALQEERAHLVHQLEDLGAAETGDLRADLDFGGSF
                     ADAAAVTAERTEVLGLVDSLKSQLDNVDRALAHIEAGTYGICDNCGNEIDPARLEFRP
                     ESVLCVDCKTKVAG"
     gene            complement(1983..>2305)
                     /locus_tag="AC161X_06245"
     CDS             complement(1983..>2305)
                     /locus_tag="AC161X_06245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HIR4989187.1"
                     /translation="PDRLSLFARGQRGDPVFLNMRGRRLTRQGAFDVVRANASRAGIE
                     AAKVSPHVLRHSAATHMVESGADLRTVQEILGHATISTTQIYTRVSATHLVEVYVQAH
                     PRSR"
BASE COUNT          442 a          731 c          711 g          421 t
ORIGIN      
        1 cgaccccccg aatctcggaa atctgacttc tcgccaccgg ctggcggtag gcgatgacag
       61 aaagggtctc gatcgcggcg ggcgagagtc ggcgtgccgt ggccgaggac gaaaaccgct
      121 ccagataggg ataggcgtcg gggtgtgaat agagacgcca ccctcccgca accgaacgga
      181 gcacgattcc tgcgcctcgt gcctccagat cgacccgcaa ctcctcgaga agttcctcga
      241 ccctcttctt cgggagctcc aacacttcgg ccagctcgcc gatcggcacc ggggtctcgg
      301 tgacaaacag gacggcttcg agagccgcgg tctcgttcat gatgcggccc attccgaggt
      361 caggtcgatg tcatcgacct cacggtgctc gacccggata tcggccagcc agtcatcctg
      421 ggttatcgca atcaggcccc aacgggcaag ctcgagcagg gccaggaagt aggcggccac
      481 ttccaccggg cgctcgcagt gctccacgag atcgtcgaaa ctcgccatgg tcatctcggc
      541 cacgcggctg cgcagctcgc cgatcgcaga ttccacggat gggaggtcga ggtcgagatg
      601 gtcgagatca ggctcggcgt ccgacctctt gaacaccctg aacgccgcag cggccagccc
      661 cgaagcgtca accggaagcg acaggtcggc gggtgcggtg acgatcctct gatcgactcc
      721 ggaggtccgc ggcacatacc gattcgcgtc ctgaagacgc cggagcagaa ccaccccgac
      781 atccttgaag gtgacacacg ccagcaggcg ggacaggagg cggtcccgct cctcgataag
      841 cgccaactcc tcatccatgt cgaagtcgcc acgacccggg agcagggatt gggccttcaa
      901 ctgaatcaac gtcgcagcga tcagtgtgaa ctcggacgtc acctcgaggt ccaagccttt
      961 catctcatcg aggaacgaga tgtactcggt cacaacctcg gtcaggccga ccgcggtgac
     1021 ttcgacctgg tgcttggaga tgagttgcag cagaaggtcc agagggcctt cgaataccgt
     1081 tgttttgaca tggaacgaca tcagttggcc actcaggata ttccgggccg gttcctggcc
     1141 agggtctttg gcggttggtc cgccttttgc agcagctccc aggtgtcgga atcgatggtg
     1201 gctgggcgct cgccgtggtg ctgacgggca aaatcgacga ttagggaatc cgagccgatg
     1261 gcctgcaatt ccaacgccgc cacctcgcca tggtctcgat aggcggccca ccgaccccga
     1321 atgggaatcc ggaacagaat caatagcgac gcgatgaccc gaccgatcgc ccccagacgg
     1381 gcatggcgct ttccgatgtc gtggagcagg gctgttctga tcagaatccg gtcctggact
     1441 cctgccgcaa ccaccgtctc cgcagccgtg tagccatgtc gctgatctgc cgccggctgg
     1501 gccaggaaca gagaattctc ttgctcggtc agccaatcct tcactgcctg ttgctctgaa
     1561 tctgcgaggg gccgtgccag caccacgtcg aagaaacgtc tggtcaggtg gctcagagag
     1621 ccgggcactc gatcagcctg cgaccttggt cttgcagtcg acgcaaagaa ccgattccgg
     1681 gcggaattcg aggcgcgccg ggtcgatttc gttgccacag ttgtcacaga tcccgtaggt
     1741 accggcttcg atgtgggcca gggctcgatc gacattgtcg agctggctct tcagcgaatc
     1801 cacaagaccg agcacttcgg ttcgctcagc agtcaccgca gccgcgtcgg cgaagctccc
     1861 gccgaagtcg agatcggccc gaaggtcacc ggtctcggca gcacccaggt cctccaactg
     1921 gtggacgagg tgtgcacgct cctcttggag tgctttctta gctctggcga tgtcgaagga
     1981 catcatcggc tcctcgggtg tgcctggacg tagacctcca cgagatgggt agcgctgacc
     2041 ctcgtgtaga tctgggtggt gctaatcgta gcgtgaccca ggatttcttg tacggtccgg
     2101 aggtcggcgc cggactccac catgtgggtt gcggccgagt gtcgcaagac gtgcggcgac
     2161 actttcgctg cctcgatccc ggctcgtgag gcgttggccc gcaccacgtc gaacgccccc
     2221 tgacgggtca gacgccgccc tctcatgttg agaaacaccg gatccccgcg ttgacctcgg
     2281 gcaaacaggc tgagccgatc cggaa
//
LOCUS       DBNHXU010000250         2244 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_78914, whole genome shotgun sequence.
ACCESSION   DBNHXU010000250 DBNHXU010000000
VERSION     DBNHXU010000250.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_78914"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1663)
                     /gene="ilvB"
                     /locus_tag="AC161X_06250"
     CDS             complement(<1..1663)
                     /gene="ilvB"
                     /locus_tag="AC161X_06250"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020020081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003984 - acetolactate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic-type acetolactate synthase large
                     subunit"
                     /protein_id="HIR4989188.1"
                     /translation="MTEKPFTGSHSLMRSLEHEGVDIVFGVPGGAILPAYDPLLDSSI
                     RHVLARHEQGAGHMAEGFAQASGRVGVVMATSGPGATNLITPLQDALMDSTPLVAITG
                     QVPTTAIGSDAFQEAYTTGLAMHCTKHSYLVTDADEIPDIVHEAFHIASTGRPGPVLV
                     DLPKDILNQITSWREPGNMKLPGYKPTTTGHPVQIKAAIDLIEAAERPVLYVGGGVIK
                     AGAAAELLAFAEAANTPVVTTLMARGSFPDNHPLALGMPGMHGLYTATTAIQKSDVLV
                     SLGARFDDRVTGDPSFFAPNAKVIHVDVDPAEIGKVRKADVPIVGDARSVLKHFLVEL
                     AARRDPDQAPARTEWLAQIGTWQQSHPLAYDQQPEGPIKVQHFIERLHAAVGGEAVIV
                     AGVGQHQMWASQFWKFNTPRSWVNSGGLGTMGFAVPAAIGAKAARPDDLVYALDGDGC
                     FQMTCQELITASTEGIPVKVAVFNNGGYGMVKQWQNLFYGGRLSAVDLTTHTPDYPML
                     AEAMGCVGLRVDHPSEIDPAIEKSRSINDKPVVLEVVVDTEEMCFP"
     gene            1863..>2244
                     /locus_tag="AC161X_06255"
     CDS             1863..>2244
                     /locus_tag="AC161X_06255"
                     /inference="COORDINATES: protein motif:HMM:NF012692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HIR4989189.1"
                     /translation="MYGIVELGGTKSLVGFGTSANDISDVLRVATTTPDETLGQLVDH
                     LRSRAGELKALGVVSFGPLELRLEHDAYGHITNTTKPGWSGASVLAPFRDAFDLEIGL
                     ETDVNGAALGEGRWGAARGLESFVY"
BASE COUNT          403 a          653 c          764 g          424 t
ORIGIN      
        1 tcggaaagca catctcctcg gtgtcgacga ccacctcgag gactaccggc ttgtcattga
       61 tggatcgaga cttctcgatg gcagggtcga tctcggaggg gtgatccacc cgtagcccca
      121 cgcagcccat cgcctcggcg agcatcggat agtcgggggt gtgagtggtt aggtccaccg
      181 cggaaaggcg accgccgtag aagaggttct gccactgctt gaccatgccg tagccgccgt
      241 tgttgaagac ggcgaccttg accgggatcc cttcggttga agcggtgatc agctcctggc
      301 aggtcatctg gaagcatcca tccccatcca gggcgtagac caggtcgtcg gggcgggcgg
      361 ctttggcgcc aatcgcggcc ggcactgcga aacccatggt ccccaggccc ccggagttga
      421 cccaggagcg gggggtgttg aacttccaga actgggaggc ccacatctga tgctggccga
      481 caccggcgac gatcacggct tctccaccga cagccgcatg caggcgctcg atgaaatgct
      541 gcaccttgat cgggccttca ggctgttggt cataggcgag aggatggctt tgctgccagg
      601 tgccgatctg agccagccac tcggtgcggg ccggcgcctg atccgggtca cggcgggcag
      661 ccagctcgac caggaaatgc ttcaggaccg agcgtgcgtc gccaacgatg ggtacgtcgg
      721 ccttgcgaac cttgccgatc tcggccggat cgacatccac atgtatgacc ttggcgttgg
      781 gggcgaagaa cgacggatct ccggtcaccc gatcgtcgaa tcgggcgcca aggctgacca
      841 ggacgtcaga cttctgaatg gctgtggtcg cggtgtagag cccatgcatt ccgggcatgc
      901 cgagggcaag agggtgattg tcagggaacg agcctcgggc catgagcgtg gtgaccaccg
      961 gagtgttggc tgcttcggcg aaggcgagca gctctgcggc cgctcccgcc ttgatcaccc
     1021 cgccgccaac gtagaggacc gggcgctctg cagcctcgat gaggtcgatg gcggccttga
     1081 tctggaccgg gtggccggta gtagtgggct tgtagcccgg aagcttcatg ttccccggct
     1141 cccgccagct ggtgatctgg ttgaggatgt ccttgggaag atcgaccagt accggccctg
     1201 gccgaccggt tgatgcgata tggaaggcct cgtggacgat gtccgggatc tcgtcggcgt
     1261 cggtcaccag atacgagtgc ttggtgcagt gcatggcgag acccgtcgtg tacgcctcct
     1321 ggaaggcgtc actcccgatg gcggtggttg ggacctggcc ggtgatggca accagaggcg
     1381 tcgagtccat cagggcgtct tggagagggg tgatcagatt ggtggccccc gggccggagg
     1441 tcgccatcac gacgccaacc cgcccgctgg cctgggcgaa tccctcggcc atgtgcccgg
     1501 cgccctgctc gtgtcttgcc aggacgtggc ggatcgaact gtcgagcagc gggtcgtagg
     1561 cggggaggat ggccccaccg ggcacgccga agacgatgtc gacgccctcg tgctcgaggc
     1621 ttcgcatcaa tgaatgtgat ccggtgaatg gtttttcagt catgttcttt caacaaaaaa
     1681 ccctccggcg ggagggttga gcgcacgcag acgtcggcgt gcgctactca atgagcagga
     1741 gtacggcgac ggtgtggttg gtcttcatgt gccctacgag taaacaacct ggtcagggag
     1801 atgtcaagat ctcccaacag caatcggtcc ctggcgccgc ctacttgagg ttgaatgccc
     1861 cgatgtacgg gatcgtcgag ctcggaggca ccaaaagcct ggtcggattc ggcacgtcgg
     1921 ccaacgacat cagcgatgtg cttcgggtcg ccaccacgac gcccgacgag acgctgggac
     1981 aactcgtgga tcacctcaga tctcgagccg gggaattgaa ggcgctgggg gtggtctcct
     2041 ttggtcctct cgaactgaga ctcgaacatg atgcctacgg gcacatcacg aacaccacca
     2101 aaccggggtg gtcaggagcc tcggtgctgg cgccatttcg cgacgcgttc gatcttgaga
     2161 tcgggctcga gacggatgtg aacggggctg cacttggcga gggccgatgg ggggctgctc
     2221 gaggtttgga gagtttcgtt tatc
//
LOCUS       DBNHXU010000251        17270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2062893, whole genome shotgun sequence.
ACCESSION   DBNHXU010000251 DBNHXU010000000
VERSION     DBNHXU010000251.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 17270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 17270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2062893"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..299)
                     /locus_tag="AC161X_06260"
     CDS             complement(<1..299)
                     /locus_tag="AC161X_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989190.1"
                     /translation="MEVLFGGLTSIFYGVADFLGGEGAKRVPAASIVLWAGIFSFPVI
                     LIVALVVGGDASLRDYLIGAGAGVVGAFGLVMLFAGLGRGHAAAVAPVSAALAAVV"
     gene            complement(300..722)
                     /locus_tag="AC161X_06265"
     CDS             complement(300..722)
                     /locus_tag="AC161X_06265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010524007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HIR4989191.1"
                     /translation="MATLFTKIIEGEIPATFVWRDDRAVGFMSINPLRPGHTLVVPRD
                     ETDHWIDCEPALREHLFDVAREIGQAQQKVYRPERIGLVIAGLEVPHLHLHVVPINSA
                     ADLDFSKAASSVDREDLEEAASSIRYILRSMGSTGVAD"
     gene            complement(751..1926)
                     /locus_tag="AC161X_06270"
     CDS             complement(751..1926)
                     /locus_tag="AC161X_06270"
                     /EC_number="1.1.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF019607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016901 - oxidoreductase activity, acting
                     on the CH-OH group of donors, quinone or similar compound
                     as acceptor [Evidence IEA];
                     GO_function: GO:0070968 - pyrroloquinoline quinone binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HIR4989192.1"
                     /translation="MRRMLICSLLVISACTGVGGVTTTASSGSSTSTSSTTPTTAPTT
                     TSTTTPAATVTTIKPLQSLAYREVAVMPFAVQITARPGDETAYIITKGGQVWAMIEGQ
                     VRSEPVLDISERVLSQGEQGMLSIALHPTDPNRFYLHYSDPQGDTVVSEFTFVSPLEA
                     DPASERVLLQVDQPAPNHNGGMLQFTPEGTLLLGLGDGGGSDDRFDNGQNPDTLLAGL
                     VELNVEGDPSPTKYATGLRNPWRFWIDEGLIYVGDVGQDIFEEIDVAPLGPGLNYGWS
                     IAEGLHCFRAEECDTTGQVIPVIEVRHDDGGACSIAGGVVYRGSLIPELDGVYLYSDY
                     CGGWLRSFRYADGVAAEQTDWTDQVGTLDTVTSFGVDGGGEVYVTTIQGLFALVPVR"
     gene            1960..3309
                     /locus_tag="AC161X_06275"
     CDS             1960..3309
                     /locus_tag="AC161X_06275"
                     /inference="COORDINATES: protein motif:HMM:NF023128.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4989193.1"
                     /translation="MTGSVKNDPRSIRSWITYDWANSAYATTILAAVLPAYFASGVVG
                     DDGVEFLGRIWTGQDLWAATVGFGPLIMFLATPILGSIADYSAAKRRFLMVFAIWGAL
                     VTCLLFFVTTGDVLLTMGLFLLADVGFVGANVFYDGLLPDLTTDDTIDMVSSRGFAWG
                     YLGGGIHLLLSLVLIQGADAGVIPIDMELATRIAIGSVGVWWLVFALKSFRGIPETGQ
                     SSILPGSPRLGWIDYIRIGFSRTVKTTRLVIHNRPLLVFLLAFILFNDGVQTTIAMAS
                     IYATETLELGITIVIVAVLLVQFVAFFGAMGFGRYASRAGTKRALFTSIAVWALVTVA
                     AFFLPVGAAAPFLLLAILIGFVLGGTQALSRSLYGSMIPEEQSAEFFGFFSVFSKFSA
                     IWGPWIFLFVRQSTGSARWAILSIALFFIVGGLLFMTVDIDQARSNRDSLRVTEATT"
     gene            3320..3649
                     /locus_tag="AC161X_06280"
     CDS             3320..3649
                     /locus_tag="AC161X_06280"
                     /inference="COORDINATES: protein motif:HMM:NF018058.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HIR4989194.1"
                     /translation="MTDESLSPIEPSEESTHGAEVTHQGSGVAWGAVVILIGLALVVV
                     FAVQNTDPVPVEFLWLSGEYPLSLVILITVAVVVLFVELFGLLYRRRRRKRLAERQEL
                     KRLRGSS"
     gene            3682..4476
                     /locus_tag="AC161X_06285"
     CDS             3682..4476
                     /locus_tag="AC161X_06285"
                     /inference="COORDINATES: protein motif:HMM:NF013125.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HIR4989195.1"
                     /translation="MVLVACSGPSEPLATQQQSESTTPNELASGEVVAIADGDTFDLA
                     TEVGEVTVRLLGVNAPERDECFHDEAAAGLELTLQSGNVTLEPYGLDQFGRTLAYTFA
                     GDTFVNLTLVESGLAIATTPDEGETRGTALLAAEEAAHSDGIGLWSPAACGGAPPNAD
                     LAIVASGVNPPGDDADVLDLERVTILNNGELETDLSDWVLRDESSAHRFQFPEGSTIG
                     PGSGLDIASDDPGWEPGEGPVWNNRGDMALLLTPGGAIVARHRFGP"
     gene            4573..4764
                     /locus_tag="AC161X_06290"
     CDS             4573..4764
                     /locus_tag="AC161X_06290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989196.1"
                     /translation="MIFGYLDAGSGSLLLQAVIGGLAGIGVAYKAMRMRIANRGLVEA
                     DTVESEVDAAEDSALPAES"
     gene            4772..6130
                     /locus_tag="AC161X_06295"
     CDS             4772..6130
                     /locus_tag="AC161X_06295"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4989197.1"
                     /translation="MIAPDPGSFRDPASRVVLDGEKVLRLLDERGLKGWRALAATEFF
                     TKAVSDGRVIAAVEVDPPAGSAGALEHPRLPLVSYPYEWTFSMLKDAALLQLDLLEEA
                     LGVGLTVKDSTPYNIQFVDGRPIFIDIGSFEAYRKGEPWIGYRQFTRQYLFPLMLRAW
                     VGVPFQPWLRGDLEGLTPSDMARLLGRRKRFNMGALLHVRLQARMESRMSDRAVREDL
                     KSAGFSSDLILANVKKLRSLVTSLVWEHGVDGWSDYGSCTHVSRDRDTKSEFLQSALD
                     RLSSKRVLDLGANDGHFSAIAAKTGAQAIALDSDEPVLDDLYRRSVGQSLSVILTDLG
                     NPSPAQGWAGGERPGIVERARPDLVIAYGLIHHLIYSASIPPQGVVAWLRSFDTPVLV
                     EFVSPKDDMVVKLSGNKLTEELHPGRTESEFRAMLEEVFEVVNEQALGEGTRILFDLE
                     PR"
     gene            6127..8223
                     /locus_tag="AC161X_06300"
     CDS             6127..8223
                     /locus_tag="AC161X_06300"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR4989198.1"
                     /translation="MSLERLGSVRWWIVPLSLATIAAFAFGQPILDLLGNNPDFFIAI
                     GMTPTDVILFPFAVLILPMVLAIPILALRWVGASTAGLAHAIAIGILFSLLAASTWIT
                     LLGGDPPVILFAVVCLGAGSAFAIEYTRYPLIQSTVTFLSLALIVFAGWFLLMTPANS
                     VAFANSSDLPEIGDVANPVPVVLVVFDEFPVASMIDSEGSLLTKEFPSFGALAADSVW
                     YRNAVGVRQQTQEALPTILTGVGAELGDVPTSADYPLNLFTLFSDSYDIAAMETITSL
                     CPSFACANSSRRIDPTAQRWGGMAVDLAVIYGHVTLPADISDHLPPINQMWGNFTSGE
                     RTNFDMIDRFMRSVEDDRREEVSRFLNILHFDSAEPSLRFGHFLFPHHPWLVTADGQL
                     TGVTYSPGSEGDGWGMDNWLIGQGYQRHLLQAQYSDTILGQSIDRLKQEGVYDDALVI
                     VLADHGVTIRAGTENERTMTPDTVGTIAAVPLFVKYPSGFPGVEPGTIDDIRAETMDI
                     MPTIAEVVGITVPWDMDGFSLLDPESRATRTESVMIGKQGTVNFGVSGKEKLDAAAEK
                     EEWFPDGDPWSLTPPGWSEWPGRSLADLDARDVPEISIKLEQQGLLDDLGQNPEVLPT
                     FLSGRVTLDEAATGSEIVVIAVDGVVRAVTRTFDPTSQSAKFQVMIAPEWLHQGSNDV
                     IAWLAVSDSKTSALVR"
     gene            complement(8241..10709)
                     /locus_tag="AC161X_06305"
     CDS             complement(8241..10709)
                     /locus_tag="AC161X_06305"
                     /inference="COORDINATES: protein motif:HMM:NF013080.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HIR4989199.1"
                     /translation="MTLPKIDRRRVWRRSLVILGLMTAGVAAPVLDLYGRNPSVFVAN
                     RSTPEQIVIFGLLVTFTLPLVAVIILTITEKISTRAADIAYYILVGISAAATGLVVSR
                     QVVPENTLGAVALAVGIMVVVLLVCRRIEGGLLFFSLLGPAVLVMFLVISPTSSLLAE
                     PAPPVSTPDQVGSPESIVFIQLDELPLASLLNEEGAINADLFPNFARLAATGNWYRNA
                     LANSIATTQSVPANLTGVLGDSDASPSVIDHPNNLFTLLGKGYEMHVVEWLTDMCPEE
                     LCKDYAGRGPARFGSLLEDVGVVYGHLSLPESARESLPSIDGSWKGFLGQAEAREAAP
                     VDIGDLAVPGPGQRSKWIDWIERIIDGVEADEPPTLHFVHLQAPHIPWRINPSGSHYQ
                     RPEQYDEVDGIEGGGKWVKRPDLPRLAYQRHMYQLGFVDNRLGALFEKLDETDNWEDT
                     LIVVLADHGASFVPGEHRRWPMENNRSDLYRIPLIIKLPGQTEGQVHDEPAYAIDVLP
                     TIVDALDITTDWDFDGHSLLQIEGTNYPHQTIHYCCSREPASTDLDELFAQVRRNHQW
                     VPNQDTWTGVAGSGPYADLVGTPVSELAPTPSDDLRWSLTQAGQLADVDRASGWVPTF
                     ISGRLELPAGESEDDLLLAVNGTVAGTGFVIRDSDTSGEIRGLLAEDLVKEGSNEVAI
                     LVPNPQGEGWLVGAAADITVDYVADDGHQLELSPEGDKRLEISAVQKTSGGWEVKGWA
                     ADVKEKLTADRIYVFAGDKLLVAGPPNLDNANVVRWFKSDKLLHSGFTFDIPDTEVPD
                     DLERLTVIAEFGDYAIESPATLPR"
     gene            10787..11086
                     /locus_tag="AC161X_06310"
     CDS             10787..11086
                     /locus_tag="AC161X_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HIR4989200.1"
                     /translation="MGFMDTVKGWFGSGKEAGSDVAAKADTAVDKAQDFASDAGATVK
                     DSTQDAYDSAKDKMTDTADSAGDAMDKASDAASDTASDAMDKAEDVVDDVKDQFS"
     gene            11163..12338
                     /locus_tag="AC161X_06315"
     CDS             11163..12338
                     /locus_tag="AC161X_06315"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013427339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HIR4989201.1"
                     /translation="MSRVSQRLGHLPPSATLGIAARARELRATGVDIISFGAGEPDFP
                     TPEHVVAAAARACFDPENHKYTAAAGLPQLRKAIAEDVARYSKVEVDWSEVMVTNGAK
                     QAVFQGFAALLDPGDEVLLPSPHWVTYPAGIDLAGGITVSVPTDLEHGFKVSVENLEQ
                     ARTDRTKLLVFVSPSNPTGAVYTAEEARAIGEWAAANDIWVMADEIYQRLTYFTDVAP
                     SIAAVTPRFENWVLINGVAKSYSMTGWRVGWMVGPADVIEAANSHQSHATGNVANVAQ
                     HAALAALNGPQEMVEEMRLAFDSRRKLMYSMISSIPGVGCIEPEGAFYVFPDFSAHLG
                     DRWATTDELAEAILEEAGVALVSGESFGTPGFLRLSYATGEDEIERGVGRIAAMLGS"
     gene            12376..13452
                     /locus_tag="AC161X_06320"
     CDS             12376..13452
                     /locus_tag="AC161X_06320"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR4989202.1"
                     /translation="MERGIKARAMMLVEPKHFTLTELPTQPVDRGGWLAVEATGISGA
                     DVQVWKGDARDVVYPLVPGHEVVGRIATEGPTIPFPVGTRVAVEPRIRCGECHRCKQS
                     LATCVRRRPSNSYGFIPSIEPPGLWGGLAEYLFIDPDARLHPLTDDVPAEVSTFVHPL
                     AAGHTWAVELPSLQTGESVLILGPGPRGLACLIAAKAAGAGWVGVTGLTHDRDRLELA
                     KRLGADLTIDVEKEDVGGAIAEAVGRRPDIIVDVTSDDPEAVFTALDLVRSGGRVILA
                     STKGNRAFHFLSDVLVAKQLTIRGAMGASSIGYQWATRQIEIDPRIDDLVSHQFPLSE
                     APRALQAAAGLLGREELISVAVTF"
     gene            13454..14728
                     /locus_tag="AC161X_06325"
     CDS             13454..14728
                     /locus_tag="AC161X_06325"
                     /inference="COORDINATES: protein motif:HMM:NF024241.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4989203.1"
                     /translation="MTVTRNGPDRLSVGSWVLYDLANTIFALGVIGLYFPDWLKSVGQ
                     RDSALALVEAGAGALVIFLAPWAGARSDVRGSRVPTLMVTTVVAVAATTTLATGPVPL
                     SLVMLGAALIAFNVGSVVYDALLVDVSTPANRGRVSGLGVGVGYFGSFIGLGLGVITL
                     DVLGWSYAATFRVLAVAFLVLALPAFFFIKERGGHDDAPLPRLRDVASRLAASWRLAS
                     THDGVVRFLVGRFLYTDAINTLIGGFLTIFVIQELGLDRQFMTTLLGVAITAAIFGAL
                     GAGRLVERIGPMRVLRNALIMWMVAIACGVAAAVTGQTNLAWVIGPLGGLALGATWTA
                     DRVVMTRISPPRHLGEFYGLYATVGRFATILGPLTWALIVDGLGWGRRVAMGALILFI
                     AVGWLTLRRVDDSERDWPPEDRLLLSESPAPH"
     gene            complement(14703..15668)
                     /locus_tag="AC161X_06330"
     CDS             complement(14703..15668)
                     /locus_tag="AC161X_06330"
                     /inference="COORDINATES: protein motif:HMM:NF015692.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HIR4989204.1"
                     /translation="MSDSESRFSLVAGEGDGAILGLAAGDSAGGAWELGYSAVTEQAT
                     VISYQLIESPDLDTDQLLRAIRELDGADDEEPVYRAETPHFRTWLTRAAAGGLVPEEE
                     PSLDAAPRGVPVGVALRRDPVALRDAVVEVGRVFHSDLSSIVSGVVTASAAAASCFGQ
                     SGRDLIAGVVETVELTATQVGSTLKYTGTATWDDLIADLRLLMDHVGVTEATDALAIV
                     DAEAVPEPWDLTRVGLLLAAPPAERAHIPIEQAARVGGSSLGAVVGGIIGARVGIRAW
                     PWAFANDTWFAEIGRRLVRGPNEVTDLPIPYAVEQHLISGERGFR"
     gene            15704..16636
                     /locus_tag="AC161X_06335"
     CDS             15704..16636
                     /locus_tag="AC161X_06335"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012981.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR4989205.1"
                     /translation="MHRIVVIANPAASQFTGGSHRDVMATLDRVADVESIWPGSPVEA
                     TEKAREAAEKGVDIIVAMGGDGMVHHVSQGVVGTSASLGVIPVGTTNVFARLMDIPSK
                     PAKAARLLVDHPQIKKVGVATLTLERGSTETVHHAIFACGFGLDAAVVQRAEKEPYRK
                     YRFGSIHYARSAFGTALQDFPRRKPHVAVHAGDHKAYATAAMIQFRDVYTYFGKIPLK
                     LETSAPAPMSVLVVKRLPRRRIPSIAAKVLAGRELDSVPELEVWREVDLFTLRAEPPV
                     EVQADGEALGMADGGSVEWAPDALAVVAPAPTQR"
     gene            complement(16623..>17270)
                     /locus_tag="AC161X_06340"
     CDS             complement(16623..>17270)
                     /locus_tag="AC161X_06340"
                     /inference="COORDINATES: protein motif:HMM:NF044972.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HIR4989206.1"
                     /translation="WLMVRRVLQFTLILFVVLMVFSIWGWSVAPFIAIGTVIAAAVGF
                     GAQSLVKDVLYGFFILAEDQYQIGDTVSLAGVTGTVEDIQFRVTVLRDVEGNVHYVAN
                     GQIAVASNFTKVYARPVIDLSISYDQDVDRAMEVMLDEMQKLEDDAEFGPMLRGPAEM
                     LGVQDFGDAGIVIRGRLNTVAHQRTAVRRAALLRVKKRFDAEGIHFSAAKDLSAG"
BASE COUNT         3117 a         5500 c         5429 g         3224 t
ORIGIN      
        1 accaccgcgg cgagagcggc cgaaaccggt gccacggccg cggcgtgccc tctaccaagc
       61 ccggcaaaga gcatcaccaa accgaatgcc ccgaccaccc ctgcccccgc gccgatcaag
      121 tagtcgcgta gagatgcgtc accaccgacc accaaggcca cgatcaaaat cactggaaaa
      181 ctgaagatcc cggcccagag gacgatcgag gccgcgggga cccgcttcgc cccctcaccc
      241 ccgaggaaat cggcaacacc gtagaagatc gaggtcaggc ccccgaaaag cacctccacc
      301 tagtcggcga ctcctgtcga tcccatcgag cggaggatgt agcgaatcga tgacgccgcc
      361 tcctcgaggt cctcgcgatc cacggaggac gccgccttcg agaaatcgag atctgccgca
      421 ctgttgatgg gcaccacatg cagatgaagg tgcggaactt caagacctgc gatcaccaac
      481 ccgatccgct ccgggcggta caccttctgc tgagcctggc cgatctcacg agccacatcg
      541 aagaggtgct cacgcaacgc gggctcgcaa tcgatccagt ggtcggtctc gtcgcggggg
      601 acaaccagag tgtgacccgg ccgcagcggg ttgatcgaca tgaacccaac cgcccggtcg
      661 tccctccaga caaaggttgc cgggatctcg ccttcgatga tcttcgtgaa cagggtggcc
      721 atgactcaat gagagcctat tcagtaactc tcagcggacg gggacgagtg caaacagccc
      781 ctggatggtg gtcacataga cctcaccgcc gccatccaca ccaaatgagg tgacggtgtc
      841 cagcgttccg acctggtcgg tccaatcggt ctgctcggct gcgacgccat cggcatatcg
      901 aaacgaccgc agccaaccac cgcaatagtc ggagtagagg taaaccccat ccaactcagg
      961 aatcaggctc ccccggtaga ccacaccgcc ggcgatcgag caggctccgc cgtcgtcgtg
     1021 acggacctcg atcacgggga ttacctgacc agtcgtgtca cattcctcag cccgaaagca
     1081 gtggaggccc tccgcaatcg accagccgta gttgagcccg ggccccaggg gagcaacgtc
     1141 gatctcctcg aagatgtcct gtccgacgtc gccgacgtag atcaggcctt cgtcgatcca
     1201 gaaccgccac ggattgcgaa gccctgtcgc gtacttggtc gggctgggat cgccttcgac
     1261 attcagctcg accagaccgg cgagcaaggt gtcagggttc tgcccgttgt cgaaccgatc
     1321 gtctgacccg ccgccatcac cgagtccgag cagaagggtc ccttcaggag tgaactggag
     1381 catccccccg ttgtggttcg gggccggctg atcgacctgg agcagcaccc gctcactcgc
     1441 cggatcggcc tcgagcggtg agacgaaggt gaactcggag acgacggtgt ccccctgagg
     1501 gtccgagtag tggagataga agcgattggg gtcggttggg tggagggcaa tagacagcat
     1561 tccttgctca ccctggctga gcacccgctc cgagatgtcg agcaccggct cagaccggac
     1621 ctggccctcg atcatggccc acacctgccc gcctttggtg atgatgtagg cggtctcgtc
     1681 cccggggcgg gccgtgattt gaacggcgaa aggcatcacc gcaacttctc gatacgcaag
     1741 tgattgcagt ggcttgatcg tggtgacagt ggcggccgga gtcgtggtgc tggtcgttgt
     1801 cggagcggta gtcggggtgg tcgagctcgt tgaggtgctg cttcccgaag aggcagtggt
     1861 cgtgactcct ccgaccccgg tgcaggcaga aatgacgagc aacgagcaga tcagcattcg
     1921 gcgcataaag cgacaggtta tgtctaggtt cccccaattg tgaccggcag cgtcaagaac
     1981 gatccacgct cgatccgcag ttggatcacc tacgactggg ccaactcggc atacgccacg
     2041 acgatcctgg cagccgtcct ccccgcctat ttcgcctccg gagtggtcgg tgacgacggg
     2101 gtcgagttct tggggagaat ttggacgggt caggacctgt gggcggccac ggtcgggttc
     2161 gggccgctga tcatgttcct ggcgacaccg attctcggct ctattgccga ctactcggct
     2221 gcgaaaaggc gattcctgat ggtctttgcg atctggggag ccctcgtgac ctgtttgctg
     2281 ttcttcgtca ccactggcga cgtactcctg acgatggggc tcttccttct cgcggatgtg
     2341 gggtttgtcg gtgccaatgt gttctacgac gggcttctgc cggacctcac caccgacgac
     2401 accatcgaca tggtcagctc gaggggattt gcctggggct atctgggcgg tggcatccac
     2461 ctcctcttgt ccctggtcct gatccaaggg gccgatgcgg gggtgatccc aatcgatatg
     2521 gagctcgcca cgaggatcgc catcggatcg gtcggggtgt ggtggctcgt cttcgccctg
     2581 aaatcgttcc gtgggatccc agagaccggg cagtcatcaa tcctgccggg gagccccagg
     2641 ctcggctgga tcgactacat acggatcggc ttctctcgaa cggtcaagac cacgcgcctg
     2701 gtcattcaca atcgaccact ccttgtcttc ctgctggcct tcatcctctt caacgacggg
     2761 gtgcagacca cgatcgcgat ggcttcgatc tacgccaccg agacactcga actggggatc
     2821 acgatcgtga tcgtcgccgt gttgctcgtc cagttcgtgg cgttcttcgg agcgatgggc
     2881 ttcggtcgct atgcaagccg ggcgggcacc aagagggcac tgttcaccag catcgcggtc
     2941 tgggcgttgg tgaccgttgc agcgttcttc ctcccggttg gggcggccgc acccttcctc
     3001 ctgttggcga ttctcatcgg gttcgtcctc gggggaaccc aggcgctgtc ccgcagcctc
     3061 tatggctcga tgatcccgga ggaacagtcg gccgagttct tcgggttctt ctcagtgttc
     3121 tcgaagttca gcgcgatctg gggaccgtgg atcttcctgt ttgtccgtca gtccaccgga
     3181 tcggcccgat gggcgatcct gtcgattgcg ctgtttttca tagtgggcgg gctgctcttc
     3241 atgaccgtcg acatcgacca agcacgcagc aaccgggatt cgcttcgggt cacggaggcg
     3301 accacgtagc cttggcccca tgaccgacga atcgctgagc cccatcgagc cctctgagga
     3361 aagcacgcac ggtgccgaag taacccatca aggttcaggg gtggcctggg gtgccgtggt
     3421 catcctcatc ggccttgcac tcgtggttgt gttcgctgta cagaacacgg atccggtgcc
     3481 ggtggagttt ctgtggctga gtggcgagta cccgttgtcg ttggtgatcc tgatcaccgt
     3541 cgcggtggtg gtgctgttcg tcgagttatt cggccttctc tatcgccgtc ggaggcgcaa
     3601 gcgactcgcc gagcgtcagg agttgaagcg tcttcgcgga tcctcctgat ccggcctgtc
     3661 cggctcgccg tggtcacagc ggtggtacta gtcgcatgct ccgggccatc cgagcccctg
     3721 gccacccagc aacagagcga gtccacgacg ccgaacgagt tggcttccgg tgaggtggtg
     3781 gccatcgcag atggcgacac ctttgacctc gcgactgaag ttggtgaggt gaccgtcaga
     3841 ttgctcggcg tcaacgcgcc cgagcgcgac gagtgcttcc acgatgaggc agcagcgggt
     3901 ctcgaactca ctctccagtc gggaaacgtc actctcgagc cctacggcct cgaccaattc
     3961 ggacggaccc tggcctacac atttgccgga gacaccttcg tcaacctgac actggtggag
     4021 tcgggacttg caatcgccac cacgcctgat gagggagaga cccggggaac agcgctgttg
     4081 gcggcggagg aggcggcaca cagcgacggg atcgggctct ggtccccggc ggcgtgcggg
     4141 ggcgcccctc cgaacgcaga tcttgccatc gtggcgtcgg gggtaaaccc tcccggtgac
     4201 gacgcggatg tgctcgacct cgaacgcgtg acgatcctca acaacggtga gctggagacg
     4261 gacctctcgg attgggtgct ccgggatgag tcatcggcgc atcgcttcca attccctgaa
     4321 ggctcgacaa tcgggcctgg atccgggctc gacatcgcaa gtgacgatcc gggatgggag
     4381 ccgggcgagg gcccggtgtg gaacaaccgc ggtgacatgg cactcctcct caccccgggt
     4441 ggtgcgattg tggcccgaca ccgattcggg ccctgatcac gcggttaggg cctggggttt
     4501 gcttcactgt cgggttggac gacaatgaag ttcggcgcct tatgtgccga tgctctcggg
     4561 aggagcaaaa acatgatctt tggatatctg gacgcaggct ccgggagtct gctccttcag
     4621 gccgtgatcg gcggtttggc aggaatcggg gtcgcttaca aggcgatgcg aatgaggatc
     4681 gccaaccgcg gtctcgtcga ggccgacact gtcgaaagcg aagtggacgc ggccgaagac
     4741 agcgcccttc cagccgaatc ctgacccagt cgtgatagcg cccgatccag ggtcgttccg
     4801 cgacccggcc tcacgggtcg tcctcgacgg tgagaaggtt ctgcgtctcc tggacgaacg
     4861 cggcctcaaa ggctggcgcg cccttgccgc gaccgagttc ttcaccaagg ccgtatccga
     4921 tggcagagtc atcgccgcgg tagaggtaga cccgcccgcc ggaagcgcgg gcgccctcga
     4981 gcatccccgc ctcccgcttg tcagctaccc ctatgagtgg acgttctcga tgctcaagga
     5041 cgcggcgctc cttcaactcg accttctcga agaagcgctc ggcgttggat tgaccgtcaa
     5101 ggacagcacc ccttacaaca tccaattcgt cgacggccgg ccgatcttca tcgacatcgg
     5161 ctctttcgag gcgtaccgaa agggagagcc gtggatcgga tatcggcagt tcacccggca
     5221 gtacctcttc ccactcatgt tgcgggcctg ggtcggggtc ccgttccagc cctggctccg
     5281 cggtgatctc gaaggtctca ctccttccga catggcgagg ctcctcggtc gccggaagcg
     5341 attcaatatg ggcgccctcc ttcacgtgcg cctccaggcc cgaatggaat cgcggatgtc
     5401 cgaccgcgcc gttagggagg atctcaagtc ggcgggattc agctcggatt tgatcctcgc
     5461 caatgtcaag aagcttcgct ctttggtgac ctctctcgtc tgggaacacg gtgtcgacgg
     5521 atggtccgac tacggctcgt gcacccacgt ctcccgggat cgagacacca agagcgagtt
     5581 cctccaatcc gcactcgatc gactgagttc gaagcgtgtc ctcgacctcg gtgccaacga
     5641 cggccacttc tcggccatcg ccgccaagac aggagctcag gcaatcgccc tcgacagcga
     5701 cgagccggtc ctcgacgacc tctatcgccg cagcgtcggc caaagcctgt ccgtcatcct
     5761 caccgacctg ggcaacccct cgccggccca gggttgggcc ggtggcgagc gtccgggaat
     5821 cgtggaacgg gcccgccccg acctcgtgat cgcctatggg ttgatccacc acctcatcta
     5881 ctcggcgagc atccctcccc agggagttgt ggcctggctt cgcagcttcg acacaccggt
     5941 cctggtcgag ttcgtatcac cgaaggacga catggtcgtc aagctttcgg ggaacaagct
     6001 caccgaggag ctgcatcctg ggcgcaccga gagtgagttc cgggcgatgc tcgaagaggt
     6061 gttcgaagtg gttaacgagc aggccctcgg cgaaggcacc aggatcctct tcgacctcga
     6121 gccgcggtga gcctggagcg gctgggatcg gtccgttggt ggatcgtccc cctctcgcta
     6181 gcaacgattg ccgcattcgc tttcgggcaa ccaatcctcg acctgctggg gaacaatccg
     6241 gacttcttca tcgccatcgg gatgaccccg acagacgtga tcctctttcc gtttgctgtc
     6301 ctgatcttgc cgatggttct cgcgattccg atcctcgccc tgcggtgggt gggggcgagc
     6361 accgccggtc tggctcatgc gatcgcgatc ggcatcctgt tctcgctgct ggcggcctcg
     6421 acttggataa cgctgcttgg cggtgaccct cccgtcatct tgtttgcagt ggtgtgcctc
     6481 ggggcggggt cggcattcgc tatcgagtac acccgttacc cactcatcca gtcaacggtc
     6541 accttcctat cgctggcgct gatcgtcttc gccggctggt tcctgctgat gacacctgca
     6601 aactccgtcg ctttcgccaa ctcgagcgac ctgcccgaga tcggcgatgt cgccaacccg
     6661 gttcccgtcg tgctcgtggt gttcgatgag tttcccgtgg catcgatgat cgattctgag
     6721 ggatcgctgt tgacgaagga gtttccgagt ttcggtgccc tggctgccga cagcgtgtgg
     6781 taccgcaatg cggtaggggt gcgccaacag acccaggaag ccctgccgac gattctcacg
     6841 ggcgtcgggg cggagttggg ggatgttccg acgtcggcgg attatccgct caacctcttc
     6901 acactcttct cggattcata cgacatcgcg gcaatggaga ccatcaccag tctctgcccg
     6961 agtttcgcct gcgcgaactc gagtcgtcgc attgacccga cggcccagcg ctggggcgga
     7021 atggccgtcg acctcgcggt gatctacggc cacgtgaccc ttcctgcaga catctccgat
     7081 cacctgcccc cgatcaacca gatgtggggc aatttcacaa gtggagagcg caccaatttc
     7141 gacatgatcg accgattcat gcgcagtgtg gaggacgacc ggcgcgaaga agtcagccgg
     7201 tttttgaata tcctccactt cgactcggcg gagccgtctc tccggtttgg ccatttcctg
     7261 tttccgcacc atccgtggct ggtgactgcc gacggacagc tcaccggggt cacctactcg
     7321 ccggggagtg agggcgatgg gtggggtatg gacaactggc tcatcggcca gggctatcag
     7381 cggcatctcc tccaggcgca atactcggac accattctcg ggcagtccat cgaccggttg
     7441 aagcaagagg gggtctacga cgacgcgttg gtaatcgtgc tcgccgacca tggggtgact
     7501 atccgagccg gcacagagaa cgagcggacc atgacgccgg atacggtcgg caccattgcc
     7561 gcggtgcctc tcttcgtcaa gtatccgagc ggattcccgg gcgtcgagcc ggggaccatc
     7621 gatgacattc gagcggagac gatggacatc atgccaacga tcgccgaggt cgtcgggatc
     7681 acagtgccct gggacatgga cggcttctcc ctgctcgatc ccgagagtag ggcgacacgc
     7741 actgaatcgg tgatgatcgg caagcaggga acggtgaact tcggcgtgtc gggaaaggag
     7801 aaactcgacg ccgccgccga gaaggaggag tggtttcccg atggtgaccc atggtcgctc
     7861 accccgccgg gatggagtga atggccgggt cggtcgcttg cagatctcga cgcccgagac
     7921 gttcccgaga tctcgatcaa acttgagcag cagggccttc tcgatgatct cggccagaac
     7981 cctgaggttc tcccgacatt cctctccggg cgggtcaccc tcgatgaagc ggccaccggt
     8041 agcgaaatcg tggtgatcgc cgtcgatggc gtggtcagag cggtaacgag gaccttcgac
     8101 cccacgtcac agtcagcgaa gttccaggtg atgattgctc ccgagtggct ccatcagggc
     8161 tccaatgacg tcatcgcctg gctggccgtt agcgacagca agacatctgc cctggtgcgc
     8221 tgacgccccg ccagtcggaa tcagcggggc agggtggcgg ggctctcgat tgcgtagtcg
     8281 ccgaactcgg cgatcacggt gagacgctcc aggtcatcgg gcacctccgt atccggaatg
     8341 tcgaaggtga atccggaatg gagcagtttg tcgctcttga accaacgcac cacgttggcg
     8401 ttgtccagat tcggcggccc ggcgaccaac aacttgtcac cggcgaacac atagatccga
     8461 tcggcggtca gtttctcctt gacgtccgcc gcccatccct tgacttccca tcctccggag
     8521 gtcttctgaa cggcgctgat ctccagtcgc ttgtcgcctt cagggctcag ttccagctga
     8581 tggccgtcgt cggcgacgta atcgaccgtg atatcggcgg cagcgcccac caaccatccc
     8641 tcgccctgcg gattgggcac gaggatcgct acttcgttgg atccctcctt caccaggtcc
     8701 tcggccaaaa gccctcgtat ctcaccgctg gtgtcgctgt ccctgatcac gaacccggtg
     8761 ccggcgacgg tgccattgac cgccaggagc aaatcgtctt ccgattcacc agccggcaat
     8821 tccaaccttc ccgagatgaa ggtgggcacc caacccgatg ctcgatccac atccgccagc
     8881 tggcccgcct gggtgagtga ccaacgcaga tcgtcgctgg gggtcggcgc caactcggag
     8941 acaggcgtcc cgaccaggtc ggcatacggg cccgacccgg ccacgccggt ccaggtgtcc
     9001 tgattcggca cccattggtg attccgtcgc acctgggcga acaactcgtc gaggtcggtg
     9061 ctcgccggct cacggctgca gcagtagtgg atcgtctggt gcgggtaatt ggtgccctcg
     9121 atctgaagga gcgagtggcc gtcgaaatcc cagtcggtag tgatgtccaa tgcgtcgaca
     9181 atggtgggaa ggacatcgat ggcgtaggca ggctcatcgt ggacttgccc ttcggtctgc
     9241 cccggcagtt tgatgatcag cgggattcgg tagaggtcgc tgcgattgtt ctccatcggc
     9301 caccgtcggt gctcccccgg gacaaaggac gctccgtggt cggcaaggac gacgatcagg
     9361 gtgtcctccc agttgtcggt ctcatcgagc ttttcgaaca gggcaccgag ccgattgtca
     9421 acgaacccaa gctgatacat gtggcgctga taggccaggc ggggcaggtc agggcgtttc
     9481 acccatttgc cgcccccctc gatcccgtcg acctcgtcgt actgctccgg ccgctggtag
     9541 tgactacccg aagggttgat cctccagggg atgtgcggag cctggagatg gacgaaatga
     9601 agcgttggcg gttcatctgc ttccactcca tcgatgatgc gttcgatcca atcgatccac
     9661 ttgctgcgct ggcccggccc agggaccgcc agatcgccga tatcgaccgg ggctgcctca
     9721 cgagcctccg cctggccgag aaagcccttc cagctcccgt cgatgctggg aaggctctcg
     9781 cgggcagatt cgggaaggga gagatggccg tagacgacac ccacgtcctc cagcagggaa
     9841 ccgaaccggg cgggcccccg acccgcgtag tccttgcaca gctcttcggg gcacatgtct
     9901 gtcagccact ccacgacgtg catctcatat cccttgccaa gaagggtgaa caggttgttg
     9961 gggtggtcga tcacggaggg gcttgcgtcc gagtcgccga ggacacccgt gaggttggcg
    10021 ggaacgctct gggtcgtggc tatcgaattg gctagcgcgt tgcgatacca attgcccgtg
    10081 gcggcgagac gggcgaaatt ggggaagaga tctgcgttga tggccccttc ctcattgagc
    10141 aacgatgcca aaggcaactc atcgagttgg atgaaaacga tcgattccgg gctccccacc
    10201 tggtccggcg tcgagacggg cggtgccggc tcggccagga gactcgatgt aggcgagatc
    10261 acgaggaaca tcaccagcac cgccgggccg agcagcgaga agaagagcag gccgccctcg
    10321 atccgccgac atacgagcag gaccaccacc atgatcccga cagccagggc caccgccccc
    10381 agggtgtttt cggggacaac ctggcgactg acgacgaggc cggtggcggc tgccgagatc
    10441 ccgaccagga tgtaataggc gatgtcggcc gcacgagtgc tgatcttctc ggtgatcgtg
    10501 aggatgatca cggcgaccag gggcagtgtg aaggtgacca aaagcccgaa tatgacgatt
    10561 tgctcagggg tgctgcggtt ggccacgaag actgagggat ttcggccgta caggtcgagc
    10621 accggtgcgg cgactccggc cgtcatcaga ccgagaatca ccaacgaacg ccgccaaaca
    10681 cgacgacgat cgatcttggg caaagtcacg ccgctcaaaa taccggtcgc caggcacaag
    10741 gtgaattgct tcgcctaggg ttgagccacg gactgaaagg accgcaatgg gattcatgga
    10801 cacagtcaag ggatggtttg gctcgggcaa ggaagccgga tcggatgtgg ccgccaaggc
    10861 agacacagcc gtcgacaaag cccaggactt cgccagcgac gccggtgcaa cggtgaaaga
    10921 ctccacacag gacgcttacg actcggccaa ggacaagatg acagatacgg ccgacagcgc
    10981 aggggacgcg atggacaagg cttccgatgc cgcctcggac accgccagtg atgcgatgga
    11041 caaggccgaa gacgtggtcg acgatgtaaa ggatcagttc tcctagacct cgacgggccc
    11101 ttcgctgctt gctaatcggt gaccgcgagc ctctgcgaca tgcttaggct gcgtccacca
    11161 ccatgagcag agtctcccaa cggctcgggc acctgccccc ctccgccacc ctgggcattg
    11221 cagcccgggc cagggaactg cgtgccacag gcgtagatat catctccttc ggcgccggag
    11281 agccggattt cccaactccg gagcacgttg tggcggcggc agctcgggcg tgtttcgacc
    11341 cggaaaacca caagtacacc gcggccgcag ggctccccca actgcgcaag gcaattgccg
    11401 aggacgtcgc tcgctactcc aaagtcgagg tcgactggtc cgaggtgatg gtcaccaacg
    11461 gcgccaaaca ggcggtgttc caggggttcg ccgcactgct cgacccgggt gacgaggtgc
    11521 tgttgccctc accgcattgg gtcacttacc ccgcaggcat cgacctcgcc gggggtatca
    11581 cggtatcggt ccccaccgat ctcgagcacg gtttcaaggt gtcggtggag aacctcgagc
    11641 aggcgaggac cgatcgcacc aagctcctgg tgtttgtctc cccttcaaat cccacgggcg
    11701 cggtttacac cgccgaggag gcaagggcga tcggggagtg ggccgcggcc aatgacatct
    11761 gggtaatggc cgacgagatc taccagcggc tcacctactt cactgacgtg gcgccatcga
    11821 ttgccgcggt cactcctcgc ttcgagaatt gggtgctgat caacggggtc gccaagagct
    11881 actcgatgac cggatggagg gtgggctgga tggtggggcc agccgatgtg atcgaggccg
    11941 ccaacagcca tcagtcgcac gcaacgggga acgtagccaa cgtggcccaa cacgcggcgc
    12001 tcgcagccct caacggccct caggagatgg ttgaggagat gcgcctcgcc ttcgactcca
    12061 ggcggaagct gatgtactcg atgatcagct cgatccccgg ggtcggctgc atcgagccgg
    12121 agggggcgtt ctatgtgttc cctgacttct ccgcgcatct cggggatcgt tgggcgacca
    12181 cagatgaact cgccgaggcc atcctcgagg aggccggagt ggcactggtt tcaggcgaat
    12241 cgttcggaac accgggattt ctcaggcttt cctatgcgac cggtgaggac gagatcgagc
    12301 ggggagtagg gcggatcgcc gcaatgctcg gcagctgaac ggagccgtcc agaaccgggt
    12361 ttacactcgc tgttgatgga gcgagggatc aaggcccggg cgatgatgct cgtcgagccc
    12421 aagcacttca cgttgaccga gttgccgact caacccgttg atcgtggtgg ttggctggcg
    12481 gtagaggcga ccggcatctc aggcgccgac gttcaggtct ggaagggtga cgcccgagat
    12541 gtcgtctatc cactcgttcc tggccacgaa gtcgtgggcc ggatcgccac cgagggcccc
    12601 acaattccgt tcccggtcgg cacccgtgtc gcagtcgagc cccgcatccg ctgtggcgag
    12661 tgccaccgct gcaagcaaag ccttgccacc tgtgtgcgtc gccggccttc caattcctac
    12721 gggttcatcc cttcgatcga gcctccaggg ctctggggcg ggctcgccga gtatctcttc
    12781 atcgatcccg acgcccgcct ccacccgctc accgacgatg tgcccgccga ggtgtcgaca
    12841 ttcgtccatc cactggctgc aggtcacacc tgggcggtcg agctgccctc attgcagacc
    12901 ggagagagcg tcctgatcct cgggcccggc cctcgcgggc tggcctgctt gatcgcagcc
    12961 aaggcggcag gagcgggctg ggtgggggta accggtctga cgcatgaccg ggaccggctc
    13021 gagctcgcca agcgcctggg ggcggacctc accattgacg tcgagaagga agacgtcggc
    13081 ggagcgatcg ccgaggcggt cggccgcagg ccggacatca tcgtcgatgt cacttcagac
    13141 gacccggaag ctgtcttcac cgccctcgac ctggtgcgga gtggcggtcg ggtgatcctg
    13201 gcgtcgacca agggcaatcg cgccttccac ttcctgagtg acgtgttggt ggccaagcag
    13261 ttgaccatcc ggggcgcgat gggtgcgagc agcatcggct accaatgggc aacgcgtcag
    13321 atcgagatcg accctcgcat cgacgatctg gtctcccacc agttccccct ctccgaagcc
    13381 ccacgcgccc tccaggcagc tgcgggtttg cttggcaggg aagagttgat atcggtcgcc
    13441 gtcaccttct gacatgaccg tgacccgtaa cggccctgat cggctttcgg tcgggtcctg
    13501 ggtcctctac gacctcgcca acaccatctt cgcccttggg gtgatcgggc tctacttccc
    13561 cgactggctg aaatccgtcg ggcagagaga cagcgctctc gccctggtgg aagcaggggc
    13621 gggggctcta gtcatctttc ttgccccctg ggccggggca cgcagtgacg tccggggaag
    13681 ccgtgtcccg accctgatgg tcaccaccgt ggtggcggta gcggccacca caaccttggc
    13741 aacaggcccc gttccgctgt cgctggtcat gctgggtgcg gcgctcatcg ccttcaatgt
    13801 gggctcggtc gtctacgacg ccctcctcgt cgatgtcagc accccggcca accgaggacg
    13861 ggtctcaggc ctgggcgttg gtgtcggcta cttcggctcc ttcatcggcc taggcctcgg
    13921 agttatcaca ctcgatgtcc tcggctggtc ctacgccgcg acattcagag tcctggcggt
    13981 cgccttcctg gtgctggccc ttcccgcctt cttcttcatc aaagagcggg gcgggcacga
    14041 cgacgcgcca ctgcccaggt tgcgggatgt ggcgagcagg ctcgccgcct cctggcgctt
    14101 ggcctccacc cacgacggag tcgtgagatt cctggtcggc cgcttcctct ataccgacgc
    14161 catcaacacc ctgatcggcg ggttcctgac catctttgtg atccaggaat tggggctcga
    14221 ccgtcagttc atgacgacgc tgcttggggt agcgatcacc gcggccatct tcggtgcgct
    14281 tggtgcgggc cggttggtgg agcggatagg cccgatgcgc gtgctgcgaa acgccctgat
    14341 catgtggatg gtcgctatcg cctgcggcgt agccgccgcc gttaccgggc aaaccaatct
    14401 ggcgtgggtg atcgggccct tgggtggcct cgccctaggc gcgacctgga ccgccgaccg
    14461 agtggtgatg acccggatct cacctccccg gcatctcggc gagttctacg gcctatatgc
    14521 cacggtgggc cgattcgcca cgatcctcgg gccgctgacc tgggccctga tcgtcgacgg
    14581 cctcggctgg ggaagacggg tggcgatggg cgccctgatc ctgttcatag ccgtcggttg
    14641 gctaaccctg cgccgggtgg acgactccga gcgggactgg ccgcccgaag accgtctgct
    14701 gctcagcgaa agccccgctc cccactgatc aggtgctgtt caacggcata aggaatcggg
    14761 aggtccgtca cctcattggg cccgcgcacc aggcggcgcc cgatctcggc aaaccaggtg
    14821 tcgttggcga aggcccatgg ccacgcccga atacccaccc gggctccgat gatccccccg
    14881 accacggctc caagagagct gcccccgacc ctggctgctt gctcgattgg gatgtgagcc
    14941 cgctccgcag gtggcgcagc cagcaacaag ccgaccctgg tcagatccca cggctcgggc
    15001 accgcctcgg catcgacgat cgcgagggcg tcggtggcct cggtcactcc gacgtggtcc
    15061 atcaacagac gaaggtcggc gatgagatcg tcccaggttg ccgtcccggt gtacttcaga
    15121 gtcgacccaa cctgagtcgc cgtcaactcc accgtctcca cgaccccggc gatcaagtcc
    15181 cggcccgact gtccgaagca cgaagcggcg gccgcgctcg cggtgaccac accggacacg
    15241 atcgacgaca gatccgagtg gaaaacacgc ccgacttcga cgacggcatc tctcagggcg
    15301 acgggatccc gccgcaacgc cacaccgacc ggcacccccc gtggcgcggc atcgagcgat
    15361 ggctcttctt cggggaccaa cccacctgct gccgcccggg tgagccatgt ccgaaagtgg
    15421 ggcgtctccg cccggtacac aggctcttcg tcgtcggcgc cgtcgagttc ccggatggcg
    15481 cgcaacagct ggtctgtgtc gagatcgggt gattcgatga gctgatacga gatgacggtg
    15541 gcttgctcgg tgacggccga gtagcccaat tcccaggcgc caccggcgct gtctccggcc
    15601 gccagaccga ggatcgcgcc atcaccctca cccgcgacca gcgaaaaccg ggattcggag
    15661 tcactcacgg ttcggcaggg taccggggta ggctcgaccc tcaatgcacc ggatagtggt
    15721 catcgccaac ccggctgcct cccagttcac cggtggatct caccgggacg tcatggcaac
    15781 actcgatcgg gtggctgacg tcgagtcgat ctggcccgga tcgccggtcg aggcgaccga
    15841 aaaggcacga gaggcggctg agaagggagt cgacatcatt gtcgccatgg gtggcgacgg
    15901 gatggtccac catgtcagtc agggagtggt ggggacctca gcctcattgg gagtcatccc
    15961 ggtggggacc accaacgtgt ttgccagatt gatggacatt ccctcgaaac cggccaaagc
    16021 cgcccggctg ttggtagacc acccacagat caagaaggtc ggtgtggcca cgttgacctt
    16081 ggagcggggg tcaacggaaa ccgtccacca tgcgatcttt gcgtgtgggt tcgggctgga
    16141 tgcggctgtg gtgcaacgtg ccgagaagga gccgtatcgc aagtatcgct tcggttcgat
    16201 ccactacgcc cgttcggcct ttggcaccgc cctccaagac ttcccccggc gcaagcccca
    16261 cgtcgccgtt cacgccggtg accacaaggc gtatgcgaca gccgccatga tccagttccg
    16321 cgacgtctac acgtacttcg ggaagattcc gttgaaactc gaaacatcgg cgcctgcccc
    16381 gatgtccgtc ctcgtggtca aacggcttcc tcggaggcga attccgagca tcgccgccaa
    16441 agtgctggcc ggtcgggagc tggactcggt ccctgagctg gaggtgtggc gggaagtcga
    16501 cctgttcacg ttgagggccg agccaccggt ggaggtccag gcggacgggg aagctttggg
    16561 gatggccgat ggcgggtcgg tcgagtgggc gccggacgcc ctcgccgtgg tcgccccagc
    16621 gcctacccag cgctgaggtc cttggcagca gagaaatgga ttccctcggc gtcgaatcgc
    16681 ttcttgaccc gaagcaaagc cgctcgtcgc accgcggttc gttggtgggc cacagtgttc
    16741 aaccggcccc ggatgactat tcccgcatca ccgaagtcct gaactccgag catctcggcc
    16801 gggcctcgaa gcatgggccc gaactcggcg tcatcctcca gcttctgcat ctcgtcgagc
    16861 atcacctcca tggcgcgatc gacgtcctgg tcataggaga tgctgaggtc gatcactggc
    16921 cgcgcataga ccttggtgaa gttggaggcg acggcgatct ggccattggc cacatagtgg
    16981 acattgccct ccacgtcccg aagcacggtc acacggaact ggatgtcctc gacggtgccg
    17041 gtcacgccgg cgagtgagac cgtgtctccg atctgatact ggtcctcggc gaggatgaag
    17101 aacccgtaga gaacgtcctt gaccaggctc tgtgcaccga agccgacggc ggcggcgatc
    17161 acggtgccga tggcaatgaa cggggccacg ctccagcccc agatcgagaa gaccatcagc
    17221 accacgaaca ggatcagcgt gaactgcaag accctgcgaa ccatcagcca
//
LOCUS       DBNHXU010000252         5909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4446003, whole genome shotgun sequence.
ACCESSION   DBNHXU010000252 DBNHXU010000000
VERSION     DBNHXU010000252.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5909
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4446003"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..802
                     /locus_tag="AC161X_06345"
     CDS             <1..802
                     /locus_tag="AC161X_06345"
                     /inference="COORDINATES: protein motif:HMM:NF012584.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2118 domain-containing protein"
                     /protein_id="HIR4989207.1"
                     /translation="SVHYDPMLAKVIAHAPTRDEAASTLANALRHARVHGLVTNRELL
                     VRILEHDDFRNGNIDTHFLDGHDLQALASPLVGLEEAHLGAIAVAIADRLHRQRQAPV
                     LSTIQPAWRNSPSQAQTVRYRWGDETLEVSYDDGVLVVSESPNVVTLDVDGDHLEFAM
                     DRFESTRFVDGPSGPLLFEEVPRFALAAVEDIPGSLHTPMPGRVVRVEVSVGDTVEEG
                     QTLMVLEAMKMEHTLRSPMTGTVVTLNATPGEQTAAGDVLVVVESQSR"
     gene            complement(815..2221)
                     /locus_tag="AC161X_06350"
     CDS             complement(815..2221)
                     /locus_tag="AC161X_06350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989208.1"
                     /translation="MAGYPNVKSGMRRITTALLGSSVLVFVACGEPASPASSLETTTA
                     SQPTGSTAPLAVSASALTCDQIDVPYAPEDWYSDTPIYVGNEQPFDEVRAFAQGLDGF
                     QDMWFDRAHNGWIGVGFTSGTDVLAQQAALEAEFPDVGIVAVEMPHTAEELAAFAAQI
                     GSRLPADMDTAGVYEVQGYVEVWVGLLTPERIAVATEAGGDAPICLSGQDPATTPLPG
                     PQSAGGEGWAYLAEADTMPASDQPQVIAEPGPLSSIWEEVGITDPMPEVDFEKQIVLV
                     LVVGHSSSCPETRLDDVVVEDDFVYAVIPTITDQMGCTSDWVPRTYLVAVDRDRLPSP
                     PFRLSAISEYGSEVLVSADLRLPGSGLAEGEIQPTSPERLRQVTPLPFWIETGIPWTL
                     TIDPACGANYLGEINGVHWHRSDGSGMPEEWKAAAVDGLVDLELMMTQGPAPTLTASA
                     GGATIRYLPGSDDGQPCA"
     gene            complement(2235..3446)
                     /locus_tag="AC161X_06355"
     CDS             complement(2235..3446)
                     /locus_tag="AC161X_06355"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020563743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR4989209.1"
                     /translation="MPHDRLDTLLSGEVEYLRAAGTSKGQEVVITEVTPSDAERGPRF
                     RLAGSDQEFLRMNSNGYLGLSRHRAVIAAEETATRDFGAGPGAVRFISGTYQLHVNLE
                     ATLAAFHGRQRAMIFSSAYAAVVSTLTSLITQETVVFSDALNHNCIINGLRTARPAAR
                     EVYPHLDFDELDRQLGQAAGTATRALVVTDGVFSMRGDHADLAAVLEVARSHDSDYPE
                     NVVVVVDDSHGVAAYGRTGRGTEEVTGSGAVDVMIGTLGKGFGVNGGYVAGTEPLVSY
                     LREKAPMYIYSNPITVGEAAASIASLEVVDSEEGVGMLTHLAAMTARFESGLVDLGFE
                     TIPGPHPVVPLMVRDTERTSSLVKHLFDHGILATGLNYPVVPKGDEEIRFQVNADHTE
                     GDIDLVLTVLS"
     gene            3484..4365
                     /locus_tag="AC161X_06360"
     CDS             3484..4365
                     /locus_tag="AC161X_06360"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006562508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR4989210.1"
                     /translation="MTRLGFIGLGIMGHGMASNLLFSGNPAMVWNRTPEKAVDLVCDV
                     ATSPQDLGEQSDIVLISVSNTSAVEEVIFGESGAFQGLREGAIIVDHSTISPSATKDF
                     ATRAEHKGVVWLDAPVSGGSEGAVNGTLAIMVGGPAEALETVRPYLETYSKSIVHVGA
                     QAGAGQMVKLMNQILVVLNELAVSEALLLGQAAGIDLATAIAAVEGGAAGSWMLSNRG
                     PQMVARDWRPGFTIDLQQKDLRLVLDTADELGVPLPGAALVFQLYRALQTRGLGQEGN
                     HALVKALEEMAGIEIGT"
     gene            complement(4432..4878)
                     /gene="bcp"
                     /locus_tag="AC161X_06365"
     CDS             complement(4432..4878)
                     /gene="bcp"
                     /locus_tag="AC161X_06365"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009885148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-dependent thiol peroxidase"
                     /protein_id="HIR4989211.1"
                     /translation="MADTVTFDLLNQDGERVSSSDFAGRRLVMFFYPAAMTPGCTLES
                     CDFRDSYQEFTAAGYDIVGVSPDKPSRNAKFREKEGLNFDLLSDEDHALADELGAWGE
                     KNLYGKTSEGLIRSTFVIGPDGAIEKAYRNIKATGHVARVKADLLG"
     gene            complement(4900..5838)
                     /locus_tag="AC161X_06370"
     CDS             complement(4900..5838)
                     /locus_tag="AC161X_06370"
                     /inference="COORDINATES: protein motif:HMM:NF015651.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HIR4989212.1"
                     /translation="MSPKRVAIIAGISAVVYIGVAVFVFNNFELPPMSTFAWAMVSAA
                     AVAQISAKWFFGLLFKESVEEAGGSLRAFSAFKGALVGAGVARLIPAGGAITPVAMAW
                     TVRDEEKGTTGAAIRTVLLNYAALLVLTGVGLLIARPKESAQILSVSLVVLAPFVLVA
                     GLLLMFGSGKLGSLKRFFPQAIRKRLDASMIDHLPDLESQVYIWARIILEAAALGLVM
                     HAFGIDVGVFQVAAAFGISSLAGGLPGTPGGLVVTEGSLVFMLTAYGFPASTTLAPVL
                     VFRIVSYWLPAALSLLAGGSIFLQSPEAKEAAAEAT"
BASE COUNT         1117 a         1830 c         1915 g         1047 t
ORIGIN      
        1 ctcggtccac tacgacccga tgctggccaa agtgatcgcc cacgccccga ccagggatga
       61 ggcggcgtcg accctggcca atgccctgcg acacgccagg gttcacggcc tggtcaccaa
      121 ccgagagttg ttggtccgaa ttctcgagca cgacgacttc cggaacggga acatcgacac
      181 tcacttcctg gatggtcacg atctccaagc cctggcctcc ccgctcgtcg gccttgagga
      241 ggctcacctg ggagcgatcg cggttgcgat cgccgatcgg ctccatcgcc agcggcaggc
      301 gccggtattg agcacgatcc agcccgcttg gcggaactcc ccgagtcaag cccagaccgt
      361 ccgttatcga tggggcgacg agacgctcga agtttcttat gacgacggag tgttagtggt
      421 ctccgagagc ccgaacgtgg tgactctcga tgtcgatggc gatcacctcg agttcgccat
      481 ggatcggttc gagtccaccc gttttgtcga cggcccctcg ggcccgttgc tgttcgagga
      541 ggtgccccga ttcgcacttg ccgccgttga ggatatccca ggctcgctcc ataccccgat
      601 gccggggcgg gtagtccgtg tcgaagtctc ggtaggggac acggttgagg agggccagac
      661 cctgatggtc ctggaggcga tgaagatgga gcacacgctg cggtcaccca tgaccgggac
      721 tgtcgtcacc ctcaatgcca ccccaggaga gcagactgcg gccggcgacg tcctggtcgt
      781 cgtggagtcc cagtcgcgct agcgagaggc aagttcaggc gcagggctgg ccgtcatccg
      841 agccgggcaa atatcggatc gtcgccccgc cggcggacgc ggtcaacgtg ggagccggcc
      901 cctgggtcat catcagctcg aggtcgacca gaccgtcgac cgcagccgcc ttccactctt
      961 cgggcattcc cgatccgtcg gaacggtgcc agtggacgcc gttgatctct ccgaggtagt
     1021 tggccccaca ggccgggtcg attgtcaggg tccacggaat tccagtctca atccagaacg
     1081 gcaacggtgt gacctggcgc aaccgctctg gtgatgtggg ctggatctcg ccctctgcga
     1141 ggccactccc cgggagccgg aggtcggcgc tgaccaggac ctcggatcca tactcgctta
     1201 tggcgctgag ccggaacggt ggcgaaggca gccgatcgcg gtccaccgcc accaaatagg
     1261 tccgaggaac ccagtcggat gtacaaccca tctgatcggt gatggtgggg atcacggcgt
     1321 aaacgaagtc atcctcgacg accacgtcat cgagccgggt ctcgggacat gagctgctgt
     1381 gcccgaccac aaggaccagg acgatctgct tctcgaagtc gacttcgggc attggatcgg
     1441 tgatgccaac ttcctcccaa atcgaggaca gtgggcctgg ctcggcaatc acctgtggct
     1501 ggtcggaggc gggcatggtg tccgcctccg ccagataggc ccagccctcg ccgccagccg
     1561 attgcggccc gggaagcggg gtggtggccg gatcctgacc ggataggcag atgggggcgt
     1621 cgcctccggc ctcagtcgcg actgcgatcc gttccggggt gaggagaccg acccagacct
     1681 cgacgtaacc ctggacctcg tagacaccag ccgtgtccat gtcggcaggt agtcgggagc
     1741 caatctgggc agcgaatgcc gctagctcct cggcagtgtg gggcatctcc acggccacaa
     1801 tcccgacatc ggggaactca gcctctagag cagcctgctg ggcgagcacg tcggtgcccg
     1861 aggtgaatcc gaccccgatc caaccgttat gagcacgatc gaaccacatg tcctggaatc
     1921 cgtcgagtcc ctgagcgaag gcccgtacct catcaaaagg ctgctcgtta ccgacataga
     1981 tgggggtgtc ggaataccag tcctcaggag cgtaagggac gtcgatctga tcgcaggtga
     2041 gggccgaggc cgagacggcg agaggggcgg tcgaaccggt gggctgtgag gcggtggtcg
     2101 tctccaagga cgaagcagga gaggctggct cgccgcaggc gacaaacacg aggactgaag
     2161 aaccaagcaa cgcggtcgtg atccggcgca tccctgattt gacgttggga tagccagcca
     2221 cggttccggt cttcctatga aaggacggtg agcaccagat cgatgtcacc ttccgtgtgg
     2281 tcggcgttga cctggaacct gatctcctcg tcgcccttgg gcacgaccgg gtaattgaga
     2341 ccggtggcca ggattccatg gtcgaagaga tgcttgacga gcgatgaggt tcgttcggtg
     2401 tctctcacca tcagcgggac caccggatgc ggaccgggaa tggtctcaaa cccgaggtcg
     2461 accagccccg actcgaacct cgcggtcatc gcggcgaggt gggtcagcat gcccacgcct
     2521 tcctccgagt cgaccacttc gagggaggca atggaggcgg ctgcctctcc gactgtgatc
     2581 gggttcgagt agatatacat cggtgccttc tctctcaggt aagagacgag gggctcggtg
     2641 cccgccacgt atccaccgtt gaccccgaat cccttcccca gagtcccgat catcacatcc
     2701 acagcgcccg atccggtcac ttcctcggtg ccccgcccgg tgcgcccata ggcggcaacc
     2761 ccatgagagt catcgacaac cacgacgacg ttctccgggt agtcagagtc gtgggaccga
     2821 gcaacctcca gaactgccgc caagtcggcg tggtcgcctc gcatcgagaa gacgccatcg
     2881 gtgaccacta gggcgcgcgt cgcagtcccg gcagcctgcc ccaattgccg atccagctcg
     2941 tcgaagtcga gatggggata gacctctcgg gccgccggtc gagccgtccg tagcccgttg
     3001 atgatgcagt tgtggttgag ggcatcggag aagaccactg tctcctgagt gatgagcgag
     3061 gtcagcgtcg agacgactgc ggcgtaggcc gaagagaaga tcatcgcccg ttgccggcca
     3121 tgaaaggctg cgagcgtggc ctcgaggttg acgtggagct gataggtgcc cgaaatgaat
     3181 ctcaccgccc cggggcctgc gccgaagtca cgagtggctg tctcctccgc ggcgatcaca
     3241 gcgcgatgac gcgacaggcc cagatatccg ttggagttca tacggaggaa ttcttggtcg
     3301 gacccggcca atcggaatcg cgggcctcgc tcagcgtcgg atggggtcac ctcggtgatg
     3361 accacctctt ggcccttgct ggtgccggcg gccctcaggt attcgacctc tccgctgaga
     3421 agggtgtcga gtcggtcgtg aggcatgacc ccacgggttt agcagagtcc tagtgtcact
     3481 cgcatgacac gtttgggctt catcgggctg ggcatcatgg gtcacgggat ggcgtccaac
     3541 cttctcttct ccgggaatcc ggcaatggtg tggaatcgga ctcctgagaa ggccgtcgac
     3601 cttgtctgtg acgtcgcaac cagccctcag gacctcggtg agcaaagcga catcgtgttg
     3661 atctcggtct ccaacacttc ggcggtcgag gaagtcattt tcggcgaatc aggcgcattt
     3721 cagggattgc gtgaaggggc gatcatcgtc gaccactcga cgatctcacc cagtgccacc
     3781 aaggatttcg ccacccgagc cgagcacaaa ggggtggttt ggctcgacgc cccggtctcg
     3841 ggtggctcag aaggtgcggt caacggcacc ctggcgatca tggtcggtgg gcccgcggag
     3901 gccctggaaa cggtgcgccc gtatctcgag acctattcga agtcgatcgt gcacgtaggg
     3961 gcccaagccg gtgcaggtca gatggtgaag ctgatgaacc agatcctggt ggtgttgaac
     4021 gagctcgcgg tctcggaggc cctcctgctc ggtcaggccg ccggcatcga cctggctact
     4081 gccattgcgg cggtcgaagg tggtgccgcg ggttcgtgga tgctcagcaa tcgtggcccg
     4141 cagatggtcg cccgtgactg gcgtcccggg ttcactatcg acctccagca gaaggacctg
     4201 cggctggttc tcgataccgc cgatgagctg ggcgtccctc tcccgggcgc cgccctcgtc
     4261 ttccagcttt acagggccct gcagacccga ggcctgggtc aagagggcaa ccacgccctg
     4321 gtcaaggcgc tcgaggaaat ggccgggatc gaaatcggga cctgagggca gtacgggcca
     4381 actgaatgcg cggcgtcaca cattggtgac gcccttcctt aggaatgcag gtcagccgag
     4441 gaggtcagcc ttcaccctcg ccacgtggcc ggtcgccttg atgttgcggt aggccttttc
     4501 gatggcgccg tcgggcccga tgacgaaggt cgagcggatc aggccctcac tggttttgcc
     4561 gtacagattc ttctcgcccc aggcacccag ctcgtcggca agggcgtggt cctcatccga
     4621 gagaagatcg aagttgaggc cttccttctc ccgaaacttc gcatttcgtg agggcttgtc
     4681 ggggctgacc ccgacgatgt catagccggc ggccgtgaac tcctggtagc tgtccctgaa
     4741 gtcacacgat tcgagcgtgc atcccggggt catcgccgcc gggtagaaga acatgacgag
     4801 acgtcggccg gcaaaatcgc tagacgacac ccgctcgccg tcctgattca agagatcgaa
     4861 ggtaactgtg tcggccatgg aacgaggcta accggttgct caggttgctt cggcggcggc
     4921 ctctttggcc tcgggagact ggaggaagat cgagcctccg gcgaggaggc tgagcgcggc
     4981 cggtagccag taggagacga tcctgaacac caacaccggc gccaatgtgg ttgaggctgg
     5041 aaagccgtat gcggtaagca tgaacaccag gctgccctcc gtgaccacca ggccgcccgg
     5101 ggtgccgggg agccctccgg caagtgatga gatgccaaac gcggcagcca cctggaagac
     5161 gccgacgtcg atcccgaagg cgtgcatgac cagtcccaga gctgcggcct cgaggatgat
     5221 ccttgcccag atgtagacct gggactccag gtcggggaga tggtcgatca tcgacgcgtc
     5281 gaggcgcttt cgaatcgcct gggggaagaa tcgcttgagg gatcccagct tccccgaccc
     5341 gaacatcagt aacaggcctg caacgagcac gaacggcgcc agcaccacaa ggctgacaga
     5401 aaggatctga gcgctctcct tggggcgggc gatcaggagg ccaaccccgg tcagtaccag
     5461 aagagcggcg tagttgagaa ggactgtccg gatcgcggca ccggtggtgc ccttctcttc
     5521 gtcacggacg gtccaagcca tggcgacggg ggtaatagcc ccccctgcgg gtatgagccg
     5581 tgcgaccccg gctccgacca gcgctccctt gaaagcactg aaagcacgga gcgacccgcc
     5641 cgcctcctct acggattcct tgaagagcag gccgaagaac cattttgcgc tgatttgggc
     5701 caccgccgcc gcggaaacca tggcccaggc gaaagtgctc atcggcggca gctcgaagtt
     5761 gttgaagacg aagacggcaa caccgatgta aacaaccgcc gagatcccgg cgatgatggc
     5821 gacccgcttc ggactcacgg ggtcagcggt tcgtcaaccg tgtgtgggaa gtcatggtcg
     5881 cccgggtcgg agtcgaagac ggccctccg
//
LOCUS       DBNHXU010000253         2966 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2063797, whole genome shotgun sequence.
ACCESSION   DBNHXU010000253 DBNHXU010000000
VERSION     DBNHXU010000253.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2966)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2966)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2966
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2063797"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(123..593)
                     /locus_tag="AC161X_06375"
     CDS             complement(123..593)
                     /locus_tag="AC161X_06375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HIR4989213.1"
                     /translation="MAHRCRRIGRGCGGSETRKPLPEVGEVIYGADRTKNRQLGHNRG
                     IDISDSTLISSQADFDKWLEAHGDNEREILVAIYNKSSGKQTVTLDELQETALRHGWI
                     DSHGQKIDEALWAIRFAPRRIGSNWSERNRRIARRLVDEGAMTAAGLAALPPDL"
     gene            585..1208
                     /locus_tag="AC161X_06380"
     CDS             585..1208
                     /locus_tag="AC161X_06380"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013426.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoE family
                     protein"
                     /protein_id="HIR4989214.1"
                     /translation="MSHWSEATQDRAREIISRYPRKRSAVMPLLYLAVAEEGYLSNEG
                     MEEVARWVEITPAQVQAVASFYTMYKRERTGRYLISCCTSISCMLLGGDDVLHAIEDE
                     SGVPHGETDEKGLLSVEHVECIGACGGAPAVQVNYELIEGVTPERARDLVRWLSGARP
                     EVINTEEMQELFGGAKSFDWGIKEESGAIGPYPAFQPFGTVGSEDRS"
     gene            1235..2518
                     /gene="nuoF"
                     /locus_tag="AC161X_06385"
     CDS             1235..2518
                     /gene="nuoF"
                     /locus_tag="AC161X_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008259446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HIR4989215.1"
                     /translation="MTDHPTDSHTMERYEATGGYRQAPRALEMTRDELVELIKKSGLL
                     GRGGAAFSTGLKWSFLAPARPAYLVINADESEPGTFKDRQLLERDPHQMLEGVIITAI
                     ANEVHHAFIYIRGEYPKPARRVQAAVDAAYAKGYLGTGIFGTDYDLEVTVHLGAGAYI
                     CGEETALINSLEGLRGEPRLKPPYFPAVKGLYMQPTIVNNVETVSDLPHILGMGAEEF
                     TDLGHPMDTGTFMTSISGHVNRPGNYELPHGILWSELIYEVAGGIRGDRELKAWVPGG
                     ASAPWFVPDVHLNQQITKDVCAEAGSMLGSGAVIVMDETTCMVRAAERIVRFFAHESC
                     GQCTPCREGTTWLEMVLRRIETGQGRQIDLDLMLDVSDGISIGLSWPPKMTTICPLGP
                     SAVSPIISLRDYFRDEVMAHVELGACPLAPSEVSA"
     gene            2515..>2966
                     /locus_tag="AC161X_06390"
     CDS             2515..>2966
                     /locus_tag="AC161X_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HIR4989216.1"
                     /translation="MTDIVNITVDGKPMEAKPGEMLIKAAQDGGTYIPRFCWHERMNP
                     VGMCRMCLVEVQGPRGPMIITACTNPVAEGMVVDTQNATVKKAQEGILEFLLANHPLD
                     CPICDKGGECPLQDQAMSHGPGESRFVEIKRNFEKPIPVSDLVLLDRER"
BASE COUNT          565 a          942 c          919 g          540 t
ORIGIN      
        1 cgccaccatc gcctcgatgg acccggtgat gggtgacgta gaccgctagg cagcgcttac
       61 ctgaggtacg tagcgccaaa tatggctcaa cgccgacaaa gaatgatggg tcgaccgaag
      121 agtcacaggt caggcggcag cgcggcgagg ccggcggccg tcatcgcccc ttcgtccacc
      181 aacctgcggg caatccttcg attccgctcc gaccagttcg agccaatacg tcgcggggca
      241 aagcggatcg cccaaagcgc ttcgtcgatc ttttgaccgt gggagtcgat ccatccgtga
      301 cgcagggcgg tttcctgcaa ttcatcgagg gtgacggtct gttttccgga cgacttattg
      361 tagatggcaa cgaggatttc ccgctcgttg tcaccgtgcg cctcgagcca tttgtcgaag
      421 tcggcttgac tggagatgag ggtggaatct gagatgtcta tcccccgatt gtgcccgagt
      481 tgcctgttct ttgttcgatc agcgccatat atgacctcac ctacctcagg taagggtttc
      541 cgggtttcgc tacccccaca gcctctccct attctccggc accgatgagc cattggtcgg
      601 aggccaccca ggaccgcgcc cgcgagatca tctcccgtta cccgaggaag cgttcggccg
      661 tgatgccgct tctctatctc gcggtcgcag aggagggcta tctgagcaac gagggcatgg
      721 aagaagtcgc ccgctgggtg gagatcaccc cggcccaggt ccaggccgtc gccagcttct
      781 acacgatgta caagagggag cggaccgggc gctacctcat ctcgtgctgc acttcgatct
      841 catgcatgct gctcggcggc gacgacgtgc ttcatgccat cgaggacgag tccggggtcc
      901 ctcacggcga gaccgatgag aagggcctgc tctccgtgga acacgtggaa tgcatcggag
      961 cctgcggtgg cgcgccggcg gtccaggtca actacgagtt gatcgaagga gttaccccgg
     1021 aacgggctcg cgacctggtc cggtggttga gcggagcccg acccgaggtg atcaacaccg
     1081 aggagatgca ggagctcttc ggaggagcca agagcttcga ttgggggatc aaggaggaat
     1141 cgggtgccat cggcccctat cccgccttcc aacccttcgg gactgtggga agcgaggatc
     1201 gttcttgacg acccctctgg tggtgacccg gcgaatgacc gaccatccga cggattccca
     1261 caccatggag cggtacgagg ccaccggcgg ctaccgacag gctccgcgtg ccctggagat
     1321 gaccagggac gagttggtgg agctgatcaa gaaatcgggc ctcctgggtc ggggaggagc
     1381 cgccttctcg accgggctca aatggagctt tctcgccccg gctcgccccg cctatctggt
     1441 gatcaatgca gacgagtccg agcccggcac cttcaaggat cgccagcttc tcgagcgtga
     1501 tccccatcag atgctggagg gggtgatcat cacggccatc gcgaacgagg ttcaccacgc
     1561 cttcatctac atccggggcg aatatccgaa gcctgctcga cgggtgcagg ccgccgttga
     1621 tgccgcttac gccaagggct atctaggcac cggcatcttc ggaaccgatt acgacctcga
     1681 ggtaactgtc catctcggag ccggtgccta catctgcggc gaggagacag ccctcatcaa
     1741 cagcctcgag ggcctccgtg gggagccgcg gctcaagccc ccctactttc ctgcggtcaa
     1801 aggcctctac atgcagccca ccatcgtcaa caacgtcgag acggtgtccg acctcccgca
     1861 catcctcggg atgggtgccg aggagttcac cgatctcggc cacccgatgg acaccggcac
     1921 gttcatgacc tcgatctccg gccatgtcaa ccgccccgga aactacgagc tgccgcacgg
     1981 gatcctgtgg agcgagttga tatatgaagt agccggcggc atccgtggcg atcgcgagct
     2041 caaggcatgg gtccctggcg gcgcctcagc gccgtggttc gtgcccgacg ttcatctcaa
     2101 ccagcagatc accaaggatg tctgtgccga ggcggggtcg atgctcggct ccggagcggt
     2161 gatcgtgatg gacgagacca cctgcatggt ccgcgctgcc gagcggatcg tccgcttctt
     2221 cgctcacgag tcgtgcggtc aatgcacccc gtgccgggaa gggaccacgt ggctggagat
     2281 ggtcctgaga cggatcgaga ccggtcaagg gcgtcagatc gatctcgacc tgatgctcga
     2341 cgtgtccgac gggatcagca tcgggctctc atggcccccg aagatgacga cgatctgccc
     2401 gttgggcccc tcggcggtgt cgcccatcat ctccttgcgt gactacttcc gggacgaggt
     2461 catggcccat gtcgaattgg gtgcctgccc cctggcgcca tccgaggtca gcgcgtgaca
     2521 gacatcgtca acatcaccgt cgacggcaag ccgatggagg ccaaacccgg cgagatgctg
     2581 atcaaggccg cccaagacgg aggcacctac atccctcgat tctgctggca cgagcggatg
     2641 aatccagtgg ggatgtgccg gatgtgcctg gtcgaagtcc agggcccccg cggtccaatg
     2701 atcatcaccg cctgcaccaa tccggtcgcc gaaggcatgg tggtcgacac ccagaacgcc
     2761 accgtgaaga aggcccagga gggaatcctc gagttccttc tcgccaacca ccccctcgac
     2821 tgcccgatat gcgacaaagg cggcgagtgc ccgctccagg accaggcaat gtcgcacggc
     2881 cccggggaaa gccggttcgt cgagatcaag aggaacttcg aaaagccgat cccggtgtcg
     2941 gacctggttc tccttgatcg cgagcg
//
LOCUS       DBNHXU010000254         5641 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3453720, whole genome shotgun sequence.
ACCESSION   DBNHXU010000254 DBNHXU010000000
VERSION     DBNHXU010000254.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5641)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5641)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5641
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3453720"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1444)
                     /locus_tag="AC161X_06395"
     CDS             complement(<1..1444)
                     /locus_tag="AC161X_06395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR4989217.1"
                     /translation="MLTALILLPVAGAAILALVPSRRRELHLPLGIALSIAPLALAGY
                     LFWVFEPSAQYQFVERAVWYEPWGISWYLGVDGISLPMIMLTALLVPIALGASTSITE
                     RTKEFVIFTLLLEAGMIGSFLALDLFLFFVFFEAILIPMYFIIGIWGGERRVYAAVKF
                     FIYTAFGSALLLAAIIALAFNHASQFGAPSFAIDDLMKLNLSSGTATWLFAAFALAFA
                     IKVPLFPFHTWLPDAHVEAPTAGSVLLAGVLLKLGTYGLLRFNVGLFPETSVDAVQIM
                     GIVAVIGIIYGAVVAIVQPDLKKLVAYSSVSHLGFIVLGTFALTSGSLQGAVIQNVNH
                     GLTTGALFLLVGMIYERRHTKQIADFGGLQKVMPLFAGFFLFMVFASIGLPGLNGFVG
                     EFLVLIGSYATLPALAVLAATGVVLAAIYLLWAYERVFTGVPDKEANLALKDLSVREI
                     SLLAPLVILVLVLGLYPAVLLDKITPSTEAV"
     gene            complement(1444..3330)
                     /gene="nuoL"
                     /locus_tag="AC161X_06400"
     CDS             complement(1444..3330)
                     /gene="nuoL"
                     /locus_tag="AC161X_06400"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014560724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR4989218.1"
                     /translation="MDTYIWLVIALPLAGAVFLHFFGRRLGEPLSGWLASAAIAGAFV
                     VALVGAIPFFEGGDHGTVTLLWQWMPSIGANLELLWDPLAALMALVVTGVGALIHVFA
                     IGYMHGDERFPRFFTYLNLFAASMLTLVLAGNYAMLFLGWELVGLCSYLLIGFWFTRP
                     TAAAAAKKAFIVNRIGDFGFVVALMLIFTTFGTLSYSGVFERAGAELTTGMATAIGLL
                     ILVGAAGKSAQIPLYVWLPDAMEGPTPVSALIHAATMVTAGVYVIARSAAIYQLSPIA
                     QTTVATIGALTAIWAASIAISQRDIKRVLAYSTISQLGYMFLAVGTTAYVAGIFHLMT
                     HAVFKALLFLGAGSVIHAMTNEQDMHKMGGLFKKVPITAITMGIATLAINGIPPFSGF
                     WSKDEILASVYARGGWFSVLWVIGLVTALMTAFYMTRQWVLVFLGEPRWDEGKHPHES
                     PRVMTTPLIVLAGLAVIAGIINTPFGTKLETFLEPSFEGISMQHPPDDAFLLVVLSGL
                     AVLAAVGGIAAAYLTYRRPKERWLDFEHGLQPIWGMWESAYRIDDLYGATLVAPGKKA
                     ADLTAFDFDVRVIDGAVNGVGRVVRNAGAWTRTLQTGYVRNYGVLFLGGALVMIIWLT
                     VGGS"
     gene            complement(3330..3632)
                     /gene="nuoK"
                     /locus_tag="AC161X_06405"
     CDS             complement(3330..3632)
                     /gene="nuoK"
                     /locus_tag="AC161X_06405"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HIR4989219.1"
                     /translation="MVASGWYMGLAAALFTIGSLGILLRRNALVMFMCIELMLNAVNL
                     TLISAGRAMNDLNGQVAVFFILVVAAAEVVVGLAIIVAIFRRRATASVDELSELKG"
     gene            complement(3632..4156)
                     /locus_tag="AC161X_06410"
     CDS             complement(3632..4156)
                     /locus_tag="AC161X_06410"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HIR4989220.1"
                     /translation="MVEALLFVTMALGALAGAVTVVIARNTVYSAMGLLATLFSVAVI
                     YVVQLAHLVAAVQVIVYAGAVMTLFLFVIMLIGVDLSEDTDERLPRQRMWVGGVAFLI
                     IVLTAAVTISGNFAWVPAAAAEPIAEATNGTVQAIAEPLFTEWVLAFEATALLLTIAA
                     AGAIALAQFRRVRT"
     gene            complement(4149..4799)
                     /gene="nuoI"
                     /locus_tag="AC161X_06415"
     CDS             complement(4149..4799)
                     /gene="nuoI"
                     /locus_tag="AC161X_06415"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019435766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HIR4989221.1"
                     /translation="MLRELIKGLRVTGGTMLRTITGEDLVTTQYPKEMRDKPQRFHGR
                     HVLNRYPDGMEKCIGCELCAGACPAKCIYVRGMDNDPEQPTSPGERYGFVYEINMLRC
                     IFCGLCVEACPTEAITMTSLFEFSTTNRQDAIYTRETLLMDKRGTVPHMFPDDELADM
                     EELNTADGWLRATAPAGVAAYEGIQMWQPTPGPADWSPEPSQEERDRRTPADDSDG"
     gene            complement(4808..>5641)
                     /locus_tag="AC161X_06420"
     CDS             complement(4808..>5641)
                     /locus_tag="AC161X_06420"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012373.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1/NuoH family protein"
                     /protein_id="HIR4989222.1"
                     /translation="IFMMVPWGKPFMIGDYEVALQGADLNVGLLFILAMSSLAVYAVV
                     LAGWSSGSKYPLLGGVRATAQAISYEAAMGLAMVAVVIFSATQGDGGTLSLAEIVERQ
                     AGNWGDLIPALEPVLRFVPRWNLIPQIVAFVIFFIAIVAETNRAPFDLVEAEQEIVGG
                     FHTEYSGMRFAMFFLAEYVNIFSMSAIAASLFLGGWNGPTFEGALPGFVSALLPTIYF
                     LMKTYFLVFMFFWIRATLPRLRYDQLMQLGWKRLIPVALGWLVVSISVLAFRQFGAPW
                     S"
BASE COUNT         1275 a         1741 c         1765 g          860 t
ORIGIN      
        1 ggaccgcctc ggtggaaggc gtgatcttgt caagcaggac cgccgggtaa agcccgagca
       61 ccaggaccag aatcaccagc ggcgccaaca gggagatctc cctgaccgag agatccttca
      121 gagcaagatt ggcttccttg tccggcaccc ctgtgaacac ccgctcgtaa gcccagagca
      181 ggtagatcgc ggccaggacg acaccggtgg cggcaagcac cgcgagggcg ggcagagtcg
      241 catagctgcc gatcagaacc aggaactcac ccacgaaacc gttgagcccg ggcaagccga
      301 tcgaggcgaa caccatgaag aggaagaacc cagcaaagag aggcatcact ttctgcagac
      361 ccccgaagtc cgcaatttgc ttggtgtggc gacgctcgta gatcatccca accagaagga
      421 agagggctcc ggtggtcagg ccgtggttga cattctgaat gacagcgccc tggaggctcc
      481 ccgacgtgag cgcgaaggtg cccagcacga tgaaaccaag gtggctcacc gacgagtagg
      541 cgacgagttt cttgagatcg ggctgaacga tcgccaccac agctccgtag atgatcccga
      601 tcaccgccac gatgcccatg atctgaacgg cgtcgaccga ggtctcgggg aacaggccaa
      661 cgttgaaccg gagcaggccg taggtgccca acttcagcag gaccccggcc aaaagcaccg
      721 atcccgccgt cggcgcctcg acgtgcgcat caggcaacca ggtgtggaac ggaaagaggg
      781 gaaccttgat cgcaaaggcc agcgcaaacg cggcgaaaag ccaggtcgcc gtccccgagc
      841 tcaggttgag cttcatcagg tcgtcgatcg cgaaggacgg cgcaccgaac tgtgatgcgt
      901 ggttgaaggc gagggcgatg atcgccgcca atagcaaggc agatccgaac gcggtgtaga
      961 tgaagaattt gaccgcggcg tagaccctcc tctccccacc ccagatcccg atgatgaagt
     1021 acatgggaat caggatcgcc tcgaagaaga cgaaaaagag aaagaggtcg agagcaagga
     1081 aagagccgat catcccggcc tcgaggagaa gggtgaagat cacgaattcc ttggttcgct
     1141 ccgtgatcga tgtcgatgcc cccagtgcaa taggcacaag cagggcagtg agcatgatca
     1201 tcggcaacga gatcccgtcc actcccaggt accagctgat cccccagggc tcgtaccaga
     1261 cggcgcgctc cacaaattgg tattgagctg acggctcgaa gacccaaaac agatagccgg
     1321 ccagagccaa cggggcgatc gagagggcga tgccgagtgg aagatgcagc tcccgacgcc
     1381 gggaggggac caaggcgagg atcgcggcgc cagccacggg aagcagaatg agggcagtca
     1441 gcatcacgac cctcccactg tcagccagat gatcatcacc agcgccccgc ccaggaacaa
     1501 caccccgtag tttcggacat aaccggtctg cagtgttcgg gtccatgcgc cggcgttccg
     1561 taccacacgt ccgacaccgt tgaccgcgcc gtcgataacc ctgacgtcaa agtcgaatgc
     1621 ggtgaggtcg gccgccttct tgcccggcgc gaccaaagtc gccccgtaca gatcatcgat
     1681 tcgataggcc gattcccaca tgccccatat cggctgaagg ccatgctcga agtcgagcca
     1741 gcgctccttg ggcctgcgat aggtgaggta tgccgcggcg atccccccaa ctgctgccag
     1801 caccgccagc ccggagagga ccaccaacaa gaaggcgtca tccggcggat gctgcatcga
     1861 gatgccttcg aagctcggct cgagaaatgt ctcgagcttg gtgccgaacg gagtgttgat
     1921 gatgccagcg atgaccgcca gaccggcgag aacgatgagc ggggtggtca tcacccgtgg
     1981 cgactcgtgc gggtgcttgc cctcgtccca gcgcggctcc ccgaggaaga ccaggaccca
     2041 ctgcctggtc atgtagaacg ccgtcatcaa tgccgtgacc agcccgatta cccagagaac
     2101 cgagaaccaa ccgcctcggg catagaccga ggccaggatc tcgtccttag accagaaccc
     2161 cgaaaacggc ggaattccgt tgatggccag ggtcgcgatg cccatcgtga tcgccgtgat
     2221 tggcaccttc ttgaacaagc cgcccatctt gtgcatgtcc tgctcattgg tcatcgcgtg
     2281 gatcaccgag cccgctccga ggaagagcaa ggccttgaag acggcatggg tcatcaggtg
     2341 gaagatcccg gctacgtagg cggtggtgcc gaccgccagg aacatgtagc cgagttggga
     2401 aatcgtggag taggcaagaa cccgcttgat gtccctttgc gagatggcaa tcgaggcggc
     2461 ccagatcgcg gttagggcac caatcgtggc gaccgtggtc tgggcgatag gcgacagttg
     2521 gtagatcgcg gccgagcgag cgatgacgta tacgccagcg gtgaccatgg ttgcggcatg
     2581 gatgagggcg gagacaggcg tcggcccctc catggcgtcg gggagccaaa cgtagagagg
     2641 gatctgggcg gacttaccgg cggcccccac caggatcaac agaccgatgg cggtggccat
     2701 cccggtggtt agctcggctc cggccctctc gaacaccccg gagtaggaga gagtgccgaa
     2761 tgtcgtgaat atgagcatca gcgccacgac gaagccgaag tccccgattc tgttgacgat
     2821 gaaggccttc ttggcggctg cagcagcggt tggccgggtg aaccagaagc cgatcagtaa
     2881 gtaggagcaa aggccgacca gttcccagcc caggaacaac atggcgtagt tgcctgccag
     2941 gaccaatgtg agcatcgacg ccgcgaagag gttcaggtag gtgaagaagc gggggaaccg
     3001 ctcatctccg tgcatgtacc cgatagcgaa gacatggatc agagcgccca cgccggtcac
     3061 caccagcgcc atcaaggcag ccaaggggtc ccagaggagc tccaggttgg ctccgatgga
     3121 cggcatccac tgccagagga gtgtgactgt cccgtgatcc ccaccctcga agaacggaat
     3181 tgcgccaacc agagcgacaa cgaatgcacc ggcgatggca gccgatgcga gccaaccact
     3241 cagaggctcg cccaggcgac gcccgaagaa gtgaaggaag accgccccgg ccagaggaag
     3301 cgcgatgacg agccagatgt aggtgtccat cagcccttga gctcgctgag ctcgtccacc
     3361 gaagcggtgg cccggcgacg gaagatggcc acgatgatgg ccaggccgac cacgacttcg
     3421 gcggcggcga ccaccaggat gaagaagacc gcaacctggc cgttgagatc gttcatggcc
     3481 cggcccgccg agatcagcgt gaggttcact gcgttgagca tcagttcgat gcacatgaac
     3541 atgaccaggg cgttgcggcg cagcaggatc cccagggagc cgatcgtgaa cagggccgcg
     3601 gccaggccca tataccaacc ggaggcgacc attacgtcct caccctccgg aactgcgcca
     3661 gcgcgattgc ccccgctgcc gcgatcgtca acagcagggc cgtggcctcg aaggcgagga
     3721 cccactcggt gaacagtggc tcggcaatgg cctggacggt gccgttggtt gcttcggcga
     3781 tcggctcagc ggctgctgcc ggcacccacg caaagttccc tgagattgtc accgccgccg
     3841 tcaggacgat gatgaggaag gcgacgccac ccacccacat ccgctggcgg ggaagtcgct
     3901 catcggtgtc ctcgctcagg tcgacgccga tgagcatgat gacaaacagg aacagagtca
     3961 tcaccgcccc ggcatagacg atgacctgga ccgcagcaac gaggtgagct agttggacca
     4021 catagatcac cgccaccgag aacagagtcg ccagcaggcc catcgccgag tacaccgtgt
     4081 tacgggcgat gaccacagtc acggctcctg cgagggcgcc gagcgccatc gtcacgaaca
     4141 gcagggcctc aaccatcgga gtcatccgct ggggtccggc gatcgcgttc ctcttgagat
     4201 ggctctggtg accaatcggc aggcccggga gttggctgcc acatctggat cccctcatag
     4261 gccgcgaccc cggcaggcgc ggtggcccgc aaccagccgt cggcagtgtt caactcctcc
     4321 atgtcggcaa gctcgtcatc agggaacatg tgggggaccg ttccccgctt gtccatgagc
     4381 agggtctccc gggtgtaaat cgcgtcctgg cggttggtgg tcgagaactc gaagagagac
     4441 gtcatcgtga tcgcctcggt cgggcaagcc tcgacacaga gcccgcagaa tatgcagcgc
     4501 agcatgttga tttcgtacac aaagccgtat cgctcaccag gcgatgtcgg ttgctccggg
     4561 tcgttgtcca tcccacgcac gtagatgcac ttggcagggc aggcgccggc gcataactcg
     4621 catccaatgc acttttccat gccatcgggg taccggttta ggacgtgccg cccgtgaaat
     4681 cgctgcggct tgtcacgcat ctccttgggg tactgagtgg tcaccaggtc ctcgccggtg
     4741 atggtgcgca acatcgtccc tccggttacc cgaagaccct tgatgagctc gcgcaacatg
     4801 cccatggcta gctccagggc gccccgaatt ggcggaacgc gagaacagag atcgagacca
     4861 cgagccaccc cagggcaacg gggatcaggc gcttccaccc gagctgcatc agctggtcat
     4921 accggagccg gggcagcgtg gcccggatcc agaagaacat gaagaccagg aaataggtct
     4981 tcatcaggaa gtagatcgtc ggaagcaggg cggagacgaa gccgggtagc gcgccctcga
     5041 aggtgggccc attccaacca ccaaggaaca gggaggccgc gatggcggac atcgaaaaga
     5101 tgttgacgta ctcggccagg aagaacatgg cgaaacgcat ccccgaatac tcggtgtgga
     5161 atcctccgac gatctcctgc tccgcttcga ccaggtcgaa gggcgctcga ttcgtctcgg
     5221 cgacgatggc gatgaagaag atcacgaagg ccacgatttg cgggatgagg ttccaacggg
     5281 gcacgaagcg gagcaccggc tccagggcag gaatcaagtc accccagttg ccagcctgtc
     5341 gctcgacgat ctcggccagt gaaagggtcc caccgtcacc ctgggttgcg ctgaaaatga
     5401 ccaccgccac catcgccagc cccatcgcag cctcgtacga gatcgcctgg gcggtggctc
     5461 ggacccctcc aaggagcggg tacttggatc ccgaagacca tcccgccagg accacggcgt
     5521 aaaccgcaag cgaggacatc gccaggatga agagcaaccc aacgttgagg tccgcgccct
     5581 gcagtgccac ctcgtagtcg ccgatcatga acggcttgcc ccagggcacc atcatgaaga
     5641 t
//
LOCUS       DBNHXU010000255         8947 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4844132, whole genome shotgun sequence.
ACCESSION   DBNHXU010000255 DBNHXU010000000
VERSION     DBNHXU010000255.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8947)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8947)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8947
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4844132"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..386)
                     /locus_tag="AC161X_06425"
     CDS             complement(<1..386)
                     /locus_tag="AC161X_06425"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0043772 - acyl-phosphate
                     glycerol-3-phosphate acyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR4989223.1"
                     /translation="MTVVAAGIIGYLIGSLPTANGLARLRGIDLRTGGSGNPGANNAR
                     RLGGTTLFIIVLVVEVLKGIMAVVIGRSLGGDWGAVFAGLGAVTGNVYNPWYRFRGGK
                     GLAISLGILLAAWPTVVPLVAVVLGVV"
     gene            complement(383..3433)
                     /locus_tag="AC161X_06430"
     CDS             complement(383..3433)
                     /locus_tag="AC161X_06430"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF024754.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR4989224.1"
                     /translation="MTQLTPSPEQAKVLELGLDTIRIRAGAGTGKTTTVAMVIANLIA
                     KHALDPERILGVTFTNKAASELADRVRSTISGSIDEGRQVEVHTYHGFAAQVLSEFGA
                     LAGVDNRVKVITPTFSRQILSETFHHTTYQHLDITNRGTLDKIRGLGDRLGDHLLDPQ
                     DLLDQIHPDDETWQSRVEMLETLTKYADDKRRLGVVDYGDLVTLSTRILQANPGLAGE
                     IRSRYEVVVLDEYQDTNPGQRVLLATIFGDRFPVIAVGDEDQTIYEWRGASSENFELF
                     PTHFRRRDGQPAKDRSLTLNRRSAQSLLEVANVIRQQANPESEPLESDDRSKKGEVIT
                     YWAGDALAEADWIARRFEELHDSGVPYAEMAVLFRKNKDFAVVVDAMARGGIPVEVAN
                     LGGLLSVPEVAELRSWLMVIERPENSAALVEILFGSRYRLGLADIAPLTNWVAGVVPV
                     SDQEDESPVTLLEAIEVIDEIGGIRDDARAALKHFLDLYRALLTESQGMSLVETCRLV
                     LDRTRAWQDIEALPATTRLTARLNLHRLLDLAEDWSPLTGRPSLEAFLDYLEAMEDEP
                     AEELDSAHLSGEDAVTLVTIHRAKGLEWDVVAIPAVTDLNFPSKARQHPDPARFAEFL
                     PVQCRIDSALADMPDDEKARAEYLKARNDTQEWRTAYVAATRARSVMMMTGAYWYGLP
                     EPTVTPKKPSPLFELVEAHPNSTSAGHEPAPDRPALLRRDLEAAAPDPVFADGWDGGL
                     RSAMADHEAMDRLADELGVKAAYDTEVETLVETLFELSATDQLPQGGADQRAVSVTGL
                     VTYAQCPKRFYWSSVDLLPRRRSRAAQVGTELHRRIELHQRGQVPFEQLSDDLYDVPD
                     EPTGSGGFKAFSNSRFAQNQAALVEAPFEIVVGDGYRVRGRIDAVYVDGGHWEVVDFK
                     SGRPSSDPSRVVQLQCYAIAASDVDFGFSTPESLDVTFAYLGGGLNEATYEADSEWVD
                     RSRSDIAGLTRSIEDKVFDETPGDWCHSCDFLRFCAPGKAWVAQ"
     gene            complement(3430..6393)
                     /locus_tag="AC161X_06435"
     CDS             complement(3430..6393)
                     /locus_tag="AC161X_06435"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012789.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0032508 - obsolete DNA duplex unwinding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR4989225.1"
                     /translation="MTVREIRVDPGAWDPVVADPDGAQLIVAGPGTGKTEFLVQRVAH
                     IVNTGRARRDQVLVLTFSRRASADMRRRVGIALGGSGVPVDATTFHSLALRLLEAGNA
                     GNRPIPLTPPEQVALVGELLTTEDPDKWPVSYRGILGTRGFAAEIADFLTRCSERLLS
                     PTDLAERAEERADWRGIAGLFLRYRQALEGVGRTDYGTLLVSAVEMLDTAKGQELAAG
                     FRYVIVDEYQDTSPAQAELARKLVRSHGTLTVAGDPYQSIYSFRGAELRNIAEFADRH
                     PELTRIVLDQSLRVPAEILSAALRVVSSGELPGSAGPVQPAGHRGRVEAYVFDQETAE
                     ADWIAREVEHVIRTEGLRPSAIAVLVRSKRELINELSRALDRRSVPHDSPDSRLVDHP
                     AVRVFDDLARIAMLEGDGRMTSPGEVADADRAMRRVLLGPLVSLGLGAERALLRERRR
                     TGAAWANVIATELPERPDLVTLLDDSSWATTLPATDGFWDAWNRLHGVERLVADPDRR
                     LWRLAISAFAQVLARQAERDEKVSLARFFEMTQDEGFESEPLLGFRGDEDRVTLTTLH
                     QSKGLEFDVVFIANAVEGVFPDLRRSRRMLRPELLSPERMTDPSAQHLFQVQEEMRLA
                     YTAMTRARLRVVWTATGAGVDQGEHRPSRFLVAAAGVGSLSELGRPAEDLRAPVTVNE
                     AEVGMRRTVFDPAESPVRRLAAARVLGSPPESWWQPAAFAGVRSAGPDRPIIGDGLRL
                     SPSQADSYATCPRRYALERRLRLGDAGSPYARFGTLVHSALERAEATILGTDEAHADL
                     EDALVELRAVWAEEADFGTPELNAAWLKQAEMALTRLYELWPSPEGRPIGLEMKVESM
                     IDGTPWMGVIDRLERTADGLKVIDYKTSKSPPTIEASKSSIQLAFYSAAVARSLEEPV
                     ATAEMWFPRTDARSITTRALDLDRLPEIETEMIEVTRSIRSEDWEPRVGDGCKNCGFR
                     KSCPAWPEGQGAFVP"
     gene            complement(6410..6553)
                     /locus_tag="AC161X_06440"
     CDS             complement(6410..6553)
                     /locus_tag="AC161X_06440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989226.1"
                     /translation="MRTKKDQTIIRADGPLDPYADPVRYLRSLGIEAELVETKEADLP
                     SAA"
     gene            6625..7917
                     /locus_tag="AC161X_06445"
     CDS             6625..7917
                     /locus_tag="AC161X_06445"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011994226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016639 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val family dehydrogenase"
                     /protein_id="HIR4989227.1"
                     /translation="MAIADTPVLKPIGHEFDSPMYHEAVGQFDRVARLMGLDDNVADR
                     LRVPQKATVVSFPFRHDNYSEVETLFGYRVQHLTTMGPTKGGIRFAPDVDLGEVAALA
                     MLMTWKCSIVGLPFGGAKGGVRLDPMQLSRSELQRVTRRFTMEIINVIGPETDIPAPD
                     LGTNEQVMAWMMDTYSQHVGHSVPAVVTGKPPALGGSVARREATGRGIMFLLPQAAAT
                     RDLDTKDATVAIHGFGNVGRYAAIAGQQMGTRVVAVSDISGAVYNPDGLDVEAMAKWV
                     DENRFLEGYPDADHIEADGVFELPVDVLVPAAVQSVIRADNADRIQAKIILEGANGPT
                     TIEADDMLRAREVLMVPDILANAGGVTVSYFEWVQDVQKYFWTENEVVARLREIMTRA
                     FSDVHNIAQSENVDMRTAALIKGIRKVADAKLVRGIFP"
     gene            complement(7914..8699)
                     /locus_tag="AC161X_06450"
     CDS             complement(7914..8699)
                     /locus_tag="AC161X_06450"
                     /EC_number="3.1.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF024117.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009338 - exodeoxyribonuclease V complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008854 - exodeoxyribonuclease V activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RecB family exonuclease"
                     /protein_id="HIR4989228.1"
                     /translation="MTVEAPDRQLTTLSPSRAGDFKTCPQLFKFRAIDRLPEPTSVYQ
                     ARGTTAHLALQRLFDHPAPDRSPDLLYNLFREAWTELRVDEEFEGLFEDTEAERTWGL
                     QSMELLANYFGVEDPTSFDPEGRELDMLEDLDGIVIRGILDRIDRDVDGRLVITDYKT
                     GSAPPERYALPAFFALKIYALLIRRRNGETPKEIRLLYLNGPTEYRLSIDDRQLDAMD
                     GQLRALWNAIERAMTTGRFPTRPGRLCDWCSFKGICPAFTEPV"
BASE COUNT         1571 a         2864 c         2920 g         1592 t
ORIGIN      
        1 acgacgccga gcaccactgc aaccagaggt accaccgtcg gccaggctgc gagcaggatc
       61 ccgagggaga tcgccagccc tttgccgcca cgaaatcgat accaggggtt gtagacgttc
      121 cctgtgaccg cccccagccc ggcgaacacc gcgccccagt ctccgcccag ggaccttcca
      181 ataacaactg ccatgatccc cttcaacacc tcgacgacca acacaatgat gaacaaggtg
      241 gtgccgccca gcctgcgggc gttgttggcg cccgggttgc ccgagcctcc ggtacgcagg
      301 tcgatccccc ggagacgggc gaggccattg gccgtgggga gggagccgat caggtagccg
      361 atgatgcccg cagccaccac cgtcattgag ccacccaggc cttccccggc gcgcaaaatc
      421 tcagaaaatc acaggaatgg caccagtcgc ccggcgtctc gtcgaagacc ttgtcctcga
      481 tggaccgggt caagccggcg atgtctgatc tcgaacggtc cacccattcg gagtcggcct
      541 cgtacgttgc ctcgttgagc ccgcctccca agtatgcgaa tgtgacgtcc aagctctcgg
      601 gcgtcgagaa gccgaagtcg acatcgctgg cggcgatggc atagcactgg agttggacga
      661 ctcgcgaggg atccgagctt ggtcgcccgc tcttgaagtc gacgacctcc cagtggccgc
      721 cgtcgacgta gacggcatcg atgcggcccc tgacccggta cccgtcaccg accactatct
      781 cgaagggtgc ttcgaccagg gcggcctgat tttgggcgaa cctggagttg ctgaatgcct
      841 tgaagccacc gctgccagtc ggctcatcgg gaacgtcgta gaggtcatcg gatagctgtt
      901 caaatggaac ctgacctcgc tggtgcagtt cgatccgccg gtgcaactcg gtgcccacct
      961 gcgccgcccg gctccgtcgt cggggcagga gatccaccga agaccaatag aagcgctttg
     1021 ggcattgggc atatgtgacg agcccggtga ccgagactgc ccgctgatcc gcacctccct
     1081 ggggaagctg gtcggtcgct gacagctcga acagagtctc caccagggtt tcgacctcag
     1141 tgtcataggc ggctttcacg ccgagctcat cggcgaggcg gtccatagcc tcatggtctg
     1201 ccatcgcgga ccgaagcccc ccgtcccacc catcggcgaa gaccgggtcc ggcgccgccg
     1261 cttcgagatc ccgccggagc aaggccgggc gatctggggc aggctcgtgc cctgcgctcg
     1321 tcgagttggg gtgggcctcc accagctcga agagcggtga tggcttcttg ggtgtcaccg
     1381 ttggctcagg aagcccgtac caataggccc cggtcatcat catcactgac cgggcccggg
     1441 tggcggctac gtaggcggtc cgccactcct gggtgtcgtt tcgtgccttg agatactcgg
     1501 cgcgggcctt ctcgtcatcg ggcatgtcgg cgagggccga gtcgatacga cattggaccg
     1561 ggagaaactc ggcgaatcgc gcaggatccg gatgctggcg cgccttgctg gggaagttca
     1621 gatcggtgac agccgggatg gccaccacgt cccactccag gcccttggcg cgatgaatgg
     1681 tcaccagggt caccgcgtcc tcaccagaga gatgggccga atcgagctcc tcggccggct
     1741 cgtcctccat cgcctcgaga tagtcgagga atgcttcgag tgacggtcgt ccggtgagcg
     1801 ggctccagtc ttcggcgagg tcgagcaacc gatgcaggtt gagtcgggcc gttagccggg
     1861 tcgtggctgg caatgcttcg atgtcctgcc aggcgcgggt cctgtccagc accaggcggc
     1921 aagtctccac caggctcatg ccctggcttt cggtcagtag agctcggtag aggtcgagga
     1981 agtgcttgag ggctgcccgg gcgtcgtcac ggatcccgcc gatctcatcg atgacctcga
     2041 ttgcctcgag aagtgtcacc gggctttcgt cctcctggtc actgaccggt accacgccgg
     2101 ccacccagtt ggtcaacggg gcgatgtcgg ccagtcccaa tcgatagcgc gatccgaaca
     2161 ggatctcgac cagagccgcc gaattctcgg ggcgctcgat caccatcagc caagagcgga
     2221 gctcggccac ttcgggcacc gacagcaacc cacccaggtt ggccacttcg acagggatcc
     2281 cgcctcgggc catggcgtcg acaaccaccg caaaatcctt gttcttgcgg aagagaactg
     2341 ccatctcggc gtaaggcacc ccgctgtcgt ggagctcctc gaaacggcgg gcgatccaat
     2401 cggcctcggc gagagcgtcg cccgcccagt aggtgatcac ctctcccttc tttgacctgt
     2461 cgtcagactc gagcggctcc gattctgggt tggcttgctg acggatcaca ttggcgactt
     2521 cgagaaggct ctgcgccgac ctccgattga gggtgaggct ccgatctttg gccggttgcc
     2581 cgtcccggcg ccgaaagtgg gtagggaaca gctcgaagtt ctccgatgac gcccctctcc
     2641 actcgtaaat cgtctggtcc tcgtcgccta ccgcgatgac agggaagcgg tcgccgaaga
     2701 tcgtggccag caacacccgt tgcccggggt tggtgtcttg gtattcgtcc agcaccacca
     2761 cctcgtaccg gctcctgatc tcgccggcaa gcccgggatt ggcctggagg atcctggtgg
     2821 acagggtgac caggtctcca tagtcgacaa ctccgagacg gcgcttgtcg tccgcatact
     2881 tggtgagggt ttccagcatc tcgacccggc tttgccaggt ctcgtcatcg gggtggatct
     2941 ggtccaagag atcctgcgga tccaatagat ggtcgccgag ccggtcgccc agccccctga
     3001 tcttgtccag ggtgccccgg ttggtgatgt cgagatgctg ataggtcgtg tgatgaaagg
     3061 tctcggacag gatctgtctc gagaaagtgg gagttatcac cttcacccgg ttgtcgacac
     3121 cggcgagagc tccgaactcg gacaggacct gagccgcgaa cccgtgatag gtgtggacct
     3181 cgacctgccg gccctcgtcg atcgatccgc tgatcgtcga gcggactcga tcggccagct
     3241 cggacgccgc cttgttggtg aaggtcacac cgagaatgcg ctcggggtcg agggcatgtt
     3301 tggcaatcag gttggcgatg accattgcca ccgtcgtcgt cttgccggtg ccggccccag
     3361 cgcggatccg gatcgtgtcg aggccgagct ccagtacttt cgcctgttcg gggctgggag
     3421 tgagctgggt catgggacga aggctccctg gccctccggc caagcagggc aggatttcct
     3481 gaatccgcag ttcttacaac cgtccccgac tctgggctcc cagtcctcag accggatgga
     3541 cctggtgact tcgatcatct cggtctcgat ttcgggcagc cggtcgagat cgagcgctct
     3601 ggtggtgatg ctcctggcat cggtcctggg aaaccacatc tcggcagtag ccaccggctc
     3661 ctcgagtgac ctggcaacgg ccgcgctgta gaaggcgagc tggatcgagc tcttcgaggc
     3721 ctcgatggtg ggcgggctct tcgaggtctt gtagtcgatg accttgaggc cgtcggcggt
     3781 gcgctccagg cgatcgataa cacccatcca cggggtgccg tcgatcatcg actcgacctt
     3841 catctcgagg ccgatcggcc gcccttccgg gctcggccac agctcataga gcctggtcag
     3901 agccatttct gcctgtttca gccacgccgc gttcaactca ggggtgccga aatcggcctc
     3961 ctccgcccag actgctctca gctcgacgag cgcatcttcg aggtcagcgt gggcttcgtc
     4021 ggtccccagg atcgttgcct cggccctttc caacgccgag tggaccaggg tgccaaaacg
     4081 ggcgtacggg gacccggcat ctcccagtcg gagccgccgt tccagggcat agcggcgggg
     4141 gcaggtggcg tacgagtcgg cctggctggg agatagcctc agcccatctc cgatgattgg
     4201 gcggtcaggg cctgcagacc ggaccccggc aaaagccgcg ggctgccacc aactctcggg
     4261 cggcgagccc aggacgcggg cagccgcgag acgacgcacc gggctttccg cagggtcgaa
     4321 caccgtccgg cgcatcccga cctcagcctc gttgactgtt accggcgccc ggaggtcttc
     4381 ggcaggacgt ccgagctcgg agagcgagcc cacgcccgcg gcggcgacca gaaagcgcga
     4441 agggcgatgc tccccctggt ccacaccggc tccggtggcg gtccagacca cacggagtcg
     4501 ggctcgggtc atagcggtgt aggcgagacg catctcctcc tgcacctgaa agaggtgctg
     4561 cgctgagggg tcggtcatcc gctccggcga cagcaactcc ggtcgcagca tccggcggct
     4621 ccggcgaaga tcgggaaaga caccctcgac ggcgttggcg atgaacacca cgtcgaattc
     4681 gagccctttc gactgatgga gggtggtcag ggtcacccgg tcttcatcgc ctcggaagcc
     4741 gagcagtggc tcggactcga agccttcgtc ctgggtcatc tcgaagaatc gggcgagact
     4801 cactttctca tctcgctccg cctgacgcgc caggacctgg gcgaaggcgc tgatggcgag
     4861 acgccacagc cgacgatccg ggtctgccac cagccgctcg acaccgtgca accggttcca
     4921 ggcatcccag aagccgtcgg tggccggcag tgtcgtggcc cagctcgaat cgtcgagcaa
     4981 tgtgacgaga tccggccgtt cggggagctc ggttgcgatg acattggccc aggcagcgcc
     5041 ggtgcgccgt cgctcccgga gcaacgccct ctccgcaccg aggcccaagg acaccagtgg
     5101 ccccagcagg acccggcgca tggcgcggtc ggcatcggca acctcacccg gcgaggtcat
     5161 ccggccgtcg ccctcgagca ttgcgatccg cgccagatcg tcgaagacgc gcaccgctgg
     5221 atggtcgacg agtctcgagt caggtgagtc gtggggcacc gatcttctgt ccagggcccg
     5281 gctcagttcg ttgatcaact cccgtttgga ccggaccagg actgcgatgg ccgacggccg
     5341 cagaccctcg gttcggatca cgtgctcgac ctcgcgagcg atccagtcgg cctcggccgt
     5401 ctcctgatcg aagacatagg cctccacccg gcctcgatgt ccggcaggct ggaccggccc
     5461 cgccgaccct ggcaactcgc ccgatgacac cacccggaga gcagcactca ggatctcggc
     5521 ggggaccctg aggctctgat ccaggacgat ccgggtcaat tcgggatgtc ggtcggcgaa
     5581 ttcggcgatg ttgcgcagtt cggcgccgcg aaacgagtag atcgactgat aggggtcgcc
     5641 ggccaccgtc agagtcccgt ggcttctaac cagctttcgg gcgagctcag cctgggccgg
     5701 cgaagtgtcc tggtactcgt cgacgatcac gtagcgaaat cctgcagcca gttcctggcc
     5761 ctttgccgtg tcgagcatct cgaccgctga cacgagcaac gttccgtagt cggttcgtcc
     5821 gacaccttcg agcgcttgcc gatagcggag gaagaggccg gcgatgcccc gccaatcggc
     5881 ccgctcctcg gcccgctcgg cgaggtcggt gggggagagg aggcgctccg agcatcgggt
     5941 gaggaagtcg gcgatctccg cggcgaagcc tcgcgtgccc aggatgccgc gatagctgac
     6001 cggccacttg tcgggatcct cggttgtgag caactcgccc accagagcca cctgttcggg
     6061 gggcgtgagt gggatgggtc gattcccggc attgcctgcc tccagcaatc tgagggccag
     6121 ggagtggaaa gtcgtggcgt cgacggggac accggaaccg ccaagggcga tcccgacccg
     6181 tcgtcgcatg tcggccgagg cccgtcgcga aaaggtgagg accaggacct ggtcccggcg
     6241 ggcgcggccg gtgttgacga tgtgggcgac ccgttgcacg aggaactcgg ttttgccggt
     6301 gcccgggccg gcgacgatca gttgggcgcc gtcgggatcc gccaccaccg ggtcccaggc
     6361 tccgggatcg acccggatct ccctgactgt caccccgcga tcgtaggtgt caggctgcag
     6421 aggggaggtc ggcttccttc gtttccacca gctcggcctc gatgccgagg cttctcaagt
     6481 agcggaccgg atcggcatag gggtccaacg gcccgtcggc ccggatgatc gtttgatcct
     6541 tcttcgttct catggtggag aaactacggc ttgcctgtga cagaaaaccg gttcgccctc
     6601 gttccggcat aggatgggag gcgtatggcc atagccgaca cgccggtgct gaagcccatc
     6661 ggccatgaat tcgactcacc gatgtatcac gaggctgtcg gccagtttga cagggtggct
     6721 cggctgatgg gactcgacga caacgtcgcc gatcggctcc gggtcccgca gaaagcgacc
     6781 gttgtcagct tcccgttccg ccatgacaac tactccgagg tcgagaccct cttcggctat
     6841 cgggtccagc atctgacgac gatggggcca accaagggcg gcatccgctt cgcacccgac
     6901 gtggacctcg gagaggtcgc cgcgcttgcg atgctgatga cctggaagtg ctcgatcgtc
     6961 gggttgccct tcggtggggc caagggcggg gttcgcctcg acccgatgca gctctcccgc
     7021 tcagagctgc agcgagtgac ccgccgattc acgatggaga tcatcaacgt catcggaccc
     7081 gagaccgaca tccccgcacc cgacctggga accaatgagc aggtgatggc atggatgatg
     7141 gacacctact cccagcacgt cggccattcg gttcctgcag tggtgaccgg caagccgcca
     7201 gccctcgggg gaagcgtggc gagacgggaa gccaccggtc gggggatcat gtttctcctc
     7261 ccacaggcgg ccgccacccg cgatctcgac accaaggacg ccaccgtggc catccacggt
     7321 ttcggcaacg tcggccggta tgccgcaatt gccggccagc agatggggac ccgggtggtg
     7381 gcggtgtccg acatctccgg cgccgtttac aaccctgacg ggctcgatgt cgaggcgatg
     7441 gcgaagtggg tcgacgagaa ccgtttcctg gagggctatc cagacgccga ccacatcgag
     7501 gctgacggcg tcttcgagct cccagtcgac gtgctggtcc cggctgccgt tcagagtgtg
     7561 atccgggccg acaatgccga caggatccag gccaagatca tcctcgaagg ggcgaacggc
     7621 ccgaccacga tcgaagccga cgacatgttg cgcgcccggg aagtgcttat ggtccccgac
     7681 atcctggcca acgcaggtgg agtgaccgtg tcctacttcg agtgggttca ggacgtgcag
     7741 aagtacttct ggaccgagaa cgaagtggtg gcccgacttc gggagatcat gacccgagcc
     7801 ttcagcgacg tgcacaacat cgcccagagt gagaacgtcg atatgaggac tgcagcgttg
     7861 atcaagggga ttcgcaaagt cgccgacgcc aagttggtac gggggatctt cccctagacc
     7921 ggctcggtga aggcggggca gatgcccttg aacgagcacc agtcgcaaag cctgcccggc
     7981 cgggtaggga acctcccggt ggtcatcgcc ctctcgatcg cgttccacag cgctcggagc
     8041 tgaccgtcca ttgcgtccaa ctgacggtcg tcgatgctga ggcgatactc ggttggcccg
     8101 ttcagataga ggagccgaat ctccttgggt gtctcaccgt tgcgacggcg gatgagaagt
     8161 gcgtagatct tcaaggcgaa gaaggcaggg agggcgtacc tctccggtgg cgccgagccg
     8221 gtcttgtagt cggtgataac cagccggcca tcgacgtccc ggtcgatccg gtcgagaatc
     8281 ccccggatca cgatcccgtc gagatcctcg agcatgtcga gctcgcggcc ttcggggtcg
     8341 aaactggtcg ggtcttcgac tccgaaatag ttggcgagca gctccattga ctgaaggccc
     8401 caggtccgct cggcctcggt gtcttcgaag agcccctcga actcctcgtc gactctcagc
     8461 tcggtccagg cctcgcggaa caggttgtag aggaggtcag gggagcgatc gggcgccggg
     8521 tggtcgaaca gccgctgtag ggcgaggtgg gccgtggtgc ctcttgcctg atacaccgag
     8581 gtgggctcgg gaagccggtc gatcgcccgg aacttgaaca actgggggca ggtcttgaag
     8641 tcgcccgccc tgctcggcga gagagtcgtg agttgtcggt cgggggcttc aacggtcatt
     8701 tgaggcgagt gtaagtaggg ggtgtgacag gaatgtggag cctgatcgat caccgcgaaa
     8761 tcaggcatta gggcgcgaaa agaaacccgc tccccggctt gacgccgcga ggggcgatac
     8821 gtacgttccc gactactgac acgggggaaa ccccggattg gagaaggaaa gacttcggtc
     8881 tttccggatc gccaccacgc cgggggcccg ctttcctggc agggtggcat gagagccccg
     8941 agagggc
//
LOCUS       DBNHXU010000256         2943 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3651955, whole genome shotgun sequence.
ACCESSION   DBNHXU010000256 DBNHXU010000000
VERSION     DBNHXU010000256.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2943
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3651955"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..269)
                     /locus_tag="AC161X_06455"
     CDS             complement(93..269)
                     /locus_tag="AC161X_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989229.1"
                     /translation="MSYEVAKSIVEEMRVSAERRSWWRRTEKDEAAPLPSWNTGMIEL
                     ETESCEDKQEMVGA"
     gene            366..1313
                     /locus_tag="AC161X_06460"
     CDS             366..1313
                     /locus_tag="AC161X_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020390032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR4989230.1"
                     /translation="MDTAERLLRLLGLLEGRIDWTADDLARRLEVTPRTVRRDITRLR
                     DLGYPVEAVAGPGGGYRLGAGGKLPPLLLDDEEALAVALGLRVATATAVGGLEDASLS
                     ALSKLEHVLPPRLRTRLEDISVATVSTVSSSSARADHNALAVAASAARTRERLRFGYT
                     DSEGRSTERHTEPLRLVHTGRRWYLVAYDLDRDDWRTFRLDRASDLRATGMRSSRRPE
                     PDPVALVARGVAIDVYRYRGVILLETNVERARQLVATTVGVVEAVDESRCRLIIGADE
                     LSWLAHFLLGLPWDFVVEEPTELRDELRAVGQRLIADHS"
     gene            complement(1304..2398)
                     /locus_tag="AC161X_06465"
     CDS             complement(1304..2398)
                     /locus_tag="AC161X_06465"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013158023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989231.1"
                     /translation="MSGVVELVNLEKRFGDMPAVDRINLKIADGEFFSLLGPSGCGKT
                     TTLRLIAGFERPDGGAILIDGVDQSGTPPHKRPVNTVFQSYALFPHMDVAANVGFGLR
                     YQNVSKAEQAKRVGEALEKVRLTGMESRKPTQLSGGQQQRVALARSLVLNPSVLLLDE
                     PLGALDAKLRKALQVELKSLQEELEITFIYVTHDQEEALTMSDRMAVMTEGKVEQLGT
                     PSEVYETPTSSYIADFLGISNLMYATAAGVGHVTLAGHRLACVSKDGATGDVTVSIRP
                     ERIELHAPDFSGDNVLNGEIERVVFAGPLLNVLVNIADVGTIQVTIPNQGATFTWDWP
                     DPVALRFPPEALRIVSETGTATEELDGDQE"
     gene            complement(2423..>2943)
                     /locus_tag="AC161X_06470"
     CDS             complement(2423..>2943)
                     /locus_tag="AC161X_06470"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR4989232.1"
                     /translation="VQPDIVCLAKALGGGLPISAIGGTDEVMGRIVDGTYDQVGTFNG
                     NPLSMAAARAMLTEVMVEDTYRHLAHIQERMEKGANEVIGRHGLPAHVATAGAKGSVI
                     YSPEPLRTYRDFLEVDGRYAQAAWLYQYNRGVFLPPWGKGEQWLVSVAHDDEDIDRYL
                     ETLDAFAALITR"
BASE COUNT          523 a          962 c          888 g          570 t
ORIGIN      
        1 gaacaccagg tcgatgtccc cgaaccgctc cggagtcaac ggtttggcga cgagctgacc
       61 tgaatcgact tcgacagaca tcgaccattc catcaggcac cgaccatttc ctgcttgtcc
      121 tcgcagctct ccgtctccaa ttcaatcatc ccggtgttcc acgacgggag cggagcggct
      181 tcgtccttct cggtccgacg ccaccaactc cgccgctcgg cactcacacg catctcctcg
      241 acgatgctct tcgcaacctc gtaactcatg aaaggactca cctttctccc ttctggatgg
      301 ctggcctaac cgtacgaaac aatgcggaca ggatctgtcc gcataattgc taaaatgtca
      361 ggcagatgga cactgcggag cgccttctca gacttctcgg cctgctggaa gggcgtatcg
      421 actggaccgc cgatgatctg gcccgaagac tggaagtcac ccctcgaacc gtgcggcgag
      481 acatcacgag actgcgcgat ctcggctatc cggtcgaggc ggtggccggt ccgggaggtg
      541 gctaccggct cggtgcaggt ggcaagctcc ctcccttgct tctcgacgac gaagaggctc
      601 tcgccgttgc gcttgggctc agggtcgcca cggcgaccgc ggtcggtggg ctcgaggatg
      661 cctcactctc ggccctgtcc aaactcgaac atgtcctgcc gccgcgcttg cggacccgac
      721 ttgaagacat ctcggtggcg accgtgtcca ccgtctcgtc gtcctcggct cgggccgatc
      781 acaacgcttt ggccgtggcc gcgtccgccg cgcgtacccg agagcgactt cgctttggat
      841 ataccgacag tgagggcagg tctacagagc gtcataccga accgctccgc ctcgtccaca
      901 ccggccgacg ctggtatctg gtcgcctacg acctcgaccg agacgactgg cgcaccttcc
      961 gattggatcg agcgtcagac ttgagggcga ccggaatgcg atcgtcccgt cgtcccgagc
     1021 ccgacccggt ggcattagtg gcccgaggtg tcgccatcga cgtctaccgg tatcgcgggg
     1081 tgatcctcct cgagaccaat gtcgaacgcg ctcgccagtt ggtagcgacc acggttggcg
     1141 tcgtcgaggc tgtcgatgag agccgctgtc gcctgatcat cggggccgac gagctgagct
     1201 ggctggctca tttcctgctg ggcctccctt gggacttcgt cgtcgaagag ccaacagaac
     1261 tgagagatga gcttcgtgcg gttggccaga ggctgatcgc cgatcactcc tgatcgccgt
     1321 cgagctcctc ggtggcggta ccggtctccg aaacgatccg aagagcctcc ggtgggaagc
     1381 gaagggccac cgggtcgggc caatcccatg tgaaggtggc gccctgattc gggatggtga
     1441 cctggatcgt tcccacgtct gcgatgttga ccaggacgtt gagcaacggc ccggcgaaca
     1501 cgaccctctc gatctccccg ttgaggacgt tatcgccgct gaagtccggc gcgtggagct
     1561 cgatacgctc agggcgaatg ctcacggtga cgtctccggt ggccccgtcc ttggagacgc
     1621 aagccagccg gtgaccagcc agggtcacat ggcccacgcc ggcggccgtg gcatacatca
     1681 gattcgagat ccccaggaag tctgcgatgt aactggaagt cggcgtctcg tagacctcgg
     1741 acggagtgcc gagctgttcc accttgccct ccgtcatcac cgccattcgg tccgacatgg
     1801 tcagagcctc ttcctggtca tgagtcacgt agatgaatgt gatctcgagc tcctcctgga
     1861 ggcttttcaa ctcgacctga agtgccttgc gcagcttggc gtcgagagcg cccagcggct
     1921 cgtcgagcag gagtaccgaa gggttgagaa ccagcgacct ggcgagggcc acccgctgct
     1981 gttggccacc ggaaagctgc gtcggcttgc gtgactccat tccggtgagc cgaaccttct
     2041 ccaaggcctc cccgaccctt ttcgcctgct ctgccttgct gacgttttgg tagcgaagac
     2101 cgaacccgac gttggcggcg acatccatgt gggggaagag ggcgtagctc tggaaaacag
     2161 tgttgaccgg gcgcttgtgg ggtggagtcc cagactggtc gacgccgtcg atcaaaatcg
     2221 ccccaccgtc agggcgctcg aatccggcga tcagtcgcag ggtcgtggtc ttgccgcatc
     2281 ccgacgggcc gagcaacgag aagaactcac catcggcgat cttgaggttg atccggtcga
     2341 ccgcaggcat gtcgccgaat ctcttctcaa gatttaccag ttcgacgaca ccgctcatca
     2401 ggcgttcgag tctaagaccg gctcatcggg tgatgagcgc cgcaaaggcg tccaaagtct
     2461 ccagataacg atcgatgtcc tcgtcgtcat gggcgaccga gaccaaccac tgttccccct
     2521 tgccccaggg gggcaagaac acgccgcggt tgtactgata gagccatgcg gcctgggcgt
     2581 aacgcccgtc gacttccagg aagtctcgat aggtccgcag cggttcgggc gagtagatga
     2641 cactcccctt ggccccggcc gtggccacgt gagccggcaa cccatgtcgc ccgatcacct
     2701 cgttagcgcc cttctccatg cgctcctgaa tgtgggcgag atgccggtag gtgtcttcga
     2761 ccatgacctc ggtcagcatc gcccgggccg cggccatgct cagggggttg ccattgaagg
     2821 ttcccacctg gtcgtaggtg ccgtccacga tgcgacccat cacctcgtcg gtcccgccga
     2881 tcgctgaaat cggtagcccg ccgcccagcg ccttcgccaa gcacacgatg tcgggttgca
     2941 ccc
//
LOCUS       DBNHXU010000257         1973 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_874777, whole genome shotgun sequence.
ACCESSION   DBNHXU010000257 DBNHXU010000000
VERSION     DBNHXU010000257.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1973)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1973
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_874777"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..528
                     /locus_tag="AC161X_06475"
     CDS             <1..528
                     /locus_tag="AC161X_06475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020509317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4389 domain-containing protein"
                     /protein_id="HIR4989233.1"
                     /translation="ATASDTVTVITDSGEVVSSITTTGLGITGGLFVATLLMIVFRQR
                     YPRWWFDFALALHRFANRVGAYVALLTDGYPSTEDEQSVHLDLDFPTVEQDLNRWMPL
                     VKWLLAIPHYIVLALLVVGAFFAILIAWFAILFTGRYPRALFDYVVGVGRWALRVEAY
                     ATLLITDQYPPFSLK"
     gene            648..1055
                     /locus_tag="AC161X_06480"
     CDS             648..1055
                     /locus_tag="AC161X_06480"
                     /inference="COORDINATES: protein motif:HMM:TIGR02246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HIR4989234.1"
                     /translation="MTHADRSPDVTAVLTARVAELQRTQMTEDVAGFLALFDEDAVWV
                     TGGGVRLVGLDAIKEFTGAVLPGAFSDGSTVTYEVVLVWSLAADVALTSVDQWYYDAG
                     GEAVSAGRPTYVWRYRDGIWSIVAGQNTAVGPA"
     gene            1154..1579
                     /locus_tag="AC161X_06485"
     CDS             1154..1579
                     /locus_tag="AC161X_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989235.1"
                     /translation="MDGLLLTVHIFAAAVWIGGGVYTSVSYPKHAANGTLRGVLAVDQ
                     KLGAIVFGSAIALLLLSGVGLVLMSDTFGFGHAFVLVGIGVIVVSSILEGTVFGPATK
                     KMLEDEGATQLPSVIKWALPVYIVLFGFTIWAMVTKLGV"
     gene            1608..>1973
                     /locus_tag="AC161X_06490"
     CDS             1608..>1973
                     /locus_tag="AC161X_06490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HIR4989236.1"
                     /translation="MTDHAVVIAGGGPTGLMLAGELALVGIDVVVVERRASQDLDGSR
                     AGGLHSRTIEVLDQRGIADRFLTEGQTHPFVGYAGTFLDISDFPTRHNYILALWQSRI
                     EPILASWVEELGVPILRQRE"
BASE COUNT          339 a          604 c          612 g          418 t
ORIGIN      
        1 gcgactgcga gcgacaccgt cacggtgatt acggacagcg gcgaggtggt ttcgagcatc
       61 actacgacgg ggttggggat caccgggggg ctgttcgtgg cgacgttgct gatgatcgtc
      121 tttcgacagc ggtatccgcg ctggtggttc gacttcgccc tcgccctcca ccggttcgcg
      181 aaccgggtcg gtgcctacgt ggcacttctc acggatgggt atccatcgac cgaagacgag
      241 caatcagtgc atctcgacct cgacttcccg acagtcgaac aggacctgaa tcgctggatg
      301 ccgctggtga aatggctgct ggccatcccc cactacatcg tcctcgcttt gctggtcgtg
      361 ggagcgttct tcgcaatcct catcgcctgg ttcgccatcc tcttcaccgg tcgttaccca
      421 cgagcactct ttgattatgt ggtcggggtg ggcagatggg ccttacgagt agaggcgtac
      481 gccaccctgc tgatcaccga ccaataccca ccgttcagct tgaaatgaac caagcaagga
      541 acaagacaac atgaccgtgg gggcccaggg acccaaatta cgccagctaa agagggcacc
      601 gacttgctgg gtttcgccga cgtcatgagg gatcggccgt tgcgcccgtg acccatgccg
      661 ataggtcccc cgacgtcacc gcggtcctca ccgcgagggt ggcggagttg caacgaactc
      721 agatgaccga agatgtggca ggtttcctcg ccctcttcga cgaggatgca gtctgggtga
      781 cgggtggagg tgtgcgcctc gtcggactcg acgcgatcaa agagttcacc ggtgccgtct
      841 taccgggagc gttctccgat gggagcaccg tcacctacga ggtcgtcctt gtttggtccc
      901 tggccgctga tgtcgcattg accagtgtcg accagtggta ctacgacgcg ggcggggaag
      961 ctgtctcggc aggtcgacca acctatgtct ggcggtatcg agacggcata tggtcgatcg
     1021 tcgccggtca gaacaccgca gtgggtccgg cgtaaccaag gcttgaaatc tcctcgtacc
     1081 gaccaccgcg ctgaatgttg atgcagtcct gtcggaactg cacccaggcc cagctcgtcg
     1141 ctagctttag gtcatggatg gtctgctact gacggtgcac atctttgccg ccgcggtctg
     1201 gatcggtggt ggcgtctaca cctcggtttc ctatccgaaa cacgccgcca acgggaccct
     1261 tcgtggagtc ttggcggttg accagaaact gggcgcaatc gtgttcggtt ccgcaatcgc
     1321 acttctgttg ttgtccggcg tcggcctcgt tctcatgtca gacacctttg ggttcggtca
     1381 tgccttcgtg ctggtcggca ttggggtgat cgtggtcagc agcatcctcg agggcacggt
     1441 cttcgggcca gccaccaaga agatgctcga agacgagggg gctacccaac ttcccagcgt
     1501 catcaagtgg gcccttcccg tgtacatcgt ccttttcggc ttcacgatct gggcgatggt
     1561 caccaagctc ggggtctaga tcgcttggcg taccggaggg tgtcgcattg acggaccatg
     1621 ccgtggtgat cgcgggcggc ggaccgaccg ggctgatgtt ggccggcgag ctggccttgg
     1681 tcgggatcga cgtcgtcgtc gtcgaacgac gcgccagtca ggatctcgac ggctctcggg
     1741 ccgggggact tcactcccgc accatcgagg tgctcgatca gcgtgggatc gccgaccgct
     1801 tcctcacgga gggtcagaca catccgttcg tcggctacgc cgggaccttc ttggacatca
     1861 gcgacttccc cacccgtcac aactacatcc tggcgctctg gcagagccgt atcgagccca
     1921 tcctggcttc ctgggtcgaa gagctcggtg tcccgatcct ccgccagcgc gag
//
LOCUS       DBNHXU010000258         4798 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4645499, whole genome shotgun sequence.
ACCESSION   DBNHXU010000258 DBNHXU010000000
VERSION     DBNHXU010000258.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4798)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4798)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4798
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4645499"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            36..1235
                     /locus_tag="AC161X_06495"
     CDS             36..1235
                     /locus_tag="AC161X_06495"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013720.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl transferase family protein"
                     /protein_id="HIR4989237.1"
                     /translation="MIRETPFHPRTSAANQTGLWSHWSGYLATEKYQTSEKFEYFAIR
                     NAAAMIDTSPLYKYRIVGRDAEHFLAGVLTRDIRKCRPGQAHYTIWCDDAGNVIEDGV
                     ILRLSDDEFLLTAAEPNLAYFRDLIGHDSVEITDVSDDYGALALQGPYAREILKTLAP
                     EMEGIGYFHLTPAKVGNAPVTISRTGYTGDLGYEIWVGRDDALDVWDAVEEIGAPMGV
                     VPAGQIALLISRIEAGLVLIDVDFYSSRYAWNDDQRTTPIELGMGWMLRDLETDDRAF
                     IGRRSIEKELKDGTSRWRTVGVMVDWEDWNRVYDERGLVPPKDHTPEHGGMMLYDSEV
                     ARIGYTPSFVYSPMMQRHIGIARVRPDHSKLGTKVSLEVTIDHRYDVVNAEVARMPFY
                     NPPRKTA"
     gene            1243..2838
                     /locus_tag="AC161X_06500"
     CDS             1243..2838
                     /locus_tag="AC161X_06500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018971786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0016116 - carotenoid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene desaturase family protein"
                     /protein_id="HIR4989238.1"
                     /translation="MADKRKYDAIVIGGGHNGLVNGSYLAKAGLDTVILERRHLVGGA
                     AITEELYPGFSFTTFSYALSLLRPEIIHELELTKHGFMPLLMPSTFAPMENGDYLHLG
                     QDKNENHHEIARHSPHDADAYDAYAHMMEQAVHAIRPLMDSIPPDITSKDPEERARLA
                     AMGSYIADLPPDVLHNLIRLLTGSAADILEDYFESDIMQGLHASSGIIGTKVGPRSQG
                     SGLVLLYHSFGEHDGAYGAWAFHKGGNGGFTQVLARAAESFGAEIRLESPVDHVITKN
                     GRAIGVMLEDGTELEAKTVVSAVDPRRTFMELVDPRELPTDLVDNIRRFKFQGTSAKV
                     NFALDANPTYPALPGRSDQYRGFINMGPSLDYLERAFDDAKYGWYSQKPYIDGCIQSN
                     VDPDMAPPGKAVMSCFIQYAPYHLKESDWDTEKDRFGDTVQATLESFFPGFGDLVLHR
                     EVVTPLDIERTVGLTEGNIFAGEFLAPQMFFFRPAPGWNQYRTPIEGYYQCGSGTHPG
                     GCVMGAPGRFASQQIIGDLAKKK"
     gene            2897..4297
                     /locus_tag="AC161X_06505"
     CDS             2897..4297
                     /locus_tag="AC161X_06505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989239.1"
                     /translation="MTQARIVLGAVAIFALSVACTRPDVTADTQPLAPPDTLTPVTTT
                     STRPQTTTTVHIDRAKIYPVDPITLEAVAGVEPISMGDFTWGISSEDGSWLAMTVGYD
                     DRTSQELRLIDVDNWVVANSWAFLVNNPIHLGDNGTVYVTDYSPTPHLSQLIPGNVIA
                     DVIADLPPHFIWYDAHFTDQTGLIFGLVSINDDNIGEAVLVTVDLATYAVDVIPLPEV
                     EIGTIGSMEIADFGPTNFDIYPQVVWGDDGSRVLIVNTNQDVVVEVDAVSGEVDEHLF
                     GLNTLNSTPTETAPFEIGQRSAVIGGASGRVLYVASGVQTFELIDEDLTTRFDPLGIE
                     AIDTETWEVIDRLDAPITDVHLSSDGNRLLATGQSYDDNPQSPQSRSSGFYVIDALDL
                     SVIAHHGGEEPDRYFGGLAMNPGLPIGYVQTWEHATSIDVVDLDVGSIVATRSGGDIQ
                     FFAEAGVLVDTSFGTS"
     gene            4445..>4798
                     /locus_tag="AC161X_06510"
     CDS             4445..>4798
                     /locus_tag="AC161X_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989240.1"
                     /translation="MAAAALAAVIALGFVFAANQGNEATESVTESRVSQVEKDAWDSR
                     VDFLTQQYHMRQASQPTVNIEKYRLEMAGLIGAITPSTSPEAYKALQQQAGKAQVNPR
                     ILVTIPAPVFSPELAR"
BASE COUNT          968 a         1527 c         1405 g          898 t
ORIGIN      
        1 acgggttatc ctgccccacc gacccgaggt agcaattgat ccgggagacg cccttccacc
       61 cgaggaccag cgccgccaac caaaccggtt tgtggagtca ctggtcggga tacctggcaa
      121 ccgagaaata ccaaacgtct gagaagttcg agtatttcgc catccgcaac gcagcggcga
      181 tgatcgacac ctcccccctc tacaagtacc ggatcgtcgg ccgcgacgcc gaacatttcc
      241 ttgccggggt cctgacccgg gacattcgaa aatgccgtcc tggtcaggcg cactacacga
      301 tctggtgcga tgacgcaggc aatgtcatcg aggacggggt cattcttcgt ctctcggatg
      361 acgagttcct actcaccgcg gccgagccca accttgccta cttccgggac ctgatcgggc
      421 acgattcggt ggagatcacc gatgtctccg acgactacgg ggccctcgca cttcaggggc
      481 cttacgccag ggagatcctc aagaccctcg cccccgaaat ggaggggatc ggctatttcc
      541 atctcacacc ggccaaggtc ggcaacgcgc cggtcaccat ctctcggacc gggtacaccg
      601 gagatctcgg gtatgagatc tgggtgggca gggacgacgc cttggacgtc tgggatgcgg
      661 tggaagagat tggcgctccg atgggcgtgg tcccggccgg gcaaatcgcc ctcctcatca
      721 gccgtatcga ggccggtctc gtcctcatcg atgtcgactt ctactcgtcg cgctatgcct
      781 ggaacgacga tcagcgaacg accccgatcg agcttgggat gggctggatg ctgcgtgatc
      841 tcgaaaccga cgaccgtgcc ttcatcgggc gtcgctccat cgagaaggag ctcaaagatg
      901 ggacttcgcg ctggcgcacc gttggggtca tggtcgactg ggaggattgg aatcgggtct
      961 acgacgagcg gggactggtg ccaccgaagg accacacccc agagcacggt gggatgatgc
     1021 tctacgactc cgaggtggcc cggattggct acaccccgag cttcgtctac tccccgatga
     1081 tgcaaaggca cattggcatc gccagggtcc ggcccgacca ctcgaaactg gggaccaagg
     1141 tcagtctcga agtcaccatc gatcatcggt atgacgtggt gaacgccgag gtggcccgca
     1201 tgcccttcta caacccgccg agaaagactg cgtgaggaac tgatggctga caaacggaaa
     1261 tacgacgcca tcgtcatcgg cggtggccac aacggcctgg tcaacgggtc gtacctggcc
     1321 aaggcgggcc tcgacaccgt catcttggag cgtcggcatc tcgttggcgg agcggcaatc
     1381 accgaagagc tctacccggg cttcagtttc accaccttct cctatgccct cagccttttg
     1441 cggccagaga tcatccacga actggagctg accaagcacg ggttcatgcc gctgctcatg
     1501 ccttcgacat tcgcccccat ggagaacggc gattacctcc atctgggaca ggacaagaac
     1561 gagaaccatc acgagatcgc caggcactca ccccatgacg ccgatgccta cgacgcatat
     1621 gcgcacatga tggagcaggc ggtccatgcc atccggcccc tcatggactc aattccgccg
     1681 gacatcacct ccaaggaccc tgaagagcgg gcccggctcg cggctatggg ctcgtatatc
     1741 gccgatctgc cacccgatgt ccttcacaat ctgatccggc tgctgacggg gagcgccgcc
     1801 gacatcctcg aggactactt cgagtccgac atcatgcagg ggctgcacgc ctcgtcgggg
     1861 atcatcggca ccaaggttgg gccccgctct cagggctcgg gcctggtgct cctctatcac
     1921 tctttcgggg agcacgatgg ggcttacggt gcttgggcct tccacaaggg tggcaacggc
     1981 ggtttcaccc aggtcctggc ccgtgcggcg gagtctttcg gtgccgagat ccggctcgag
     2041 tcaccagtgg atcacgtcat caccaagaac ggccgggcga tcggcgtgat gctcgaagac
     2101 gggactgagt tggaggccaa gaccgtggtc agcgctgtcg acccccggcg caccttcatg
     2161 gaactggtcg acccccgcga attgcccacc gacctggtcg acaacatccg ccgcttcaag
     2221 ttccagggaa catccgccaa ggtgaacttc gccctcgacg ccaacccgac ctacccggcc
     2281 ctccctgggc gatcagacca gtaccggggc ttcatcaaca tgggaccgtc gctggactat
     2341 ttggagcgag ccttcgacga cgccaagtac ggctggtact cccaaaagcc ctacatcgac
     2401 gggtgcatcc agtccaatgt cgatccggac atggcgcctc caggcaaggc ggtgatgtcc
     2461 tgtttcatcc agtacgcccc atatcacctc aaggaaagtg actgggacac ggagaaggat
     2521 cgattcggtg acaccgtcca agcaactctg gagtcgttct tccccgggtt cggcgatctg
     2581 gtcctgcacc gagaagtggt caccccccta gacatcgagc gcaccgttgg cctcaccgaa
     2641 ggcaacatct tcgctgggga gttcctggcc ccccagatgt tcttcttccg accggcgccg
     2701 ggctggaacc agtaccggac cccgatcgag ggctactacc agtgtggttc gggcacccat
     2761 cccggtgggt gcgtgatggg agctcccggc cggttcgcct cccagcagat catcggggac
     2821 ctcgccaaga agaagtagcg aggggaacgc ctccgaacga gtcgccgttg atagcgttga
     2881 ctcgggagga tctcccatga cgcaagcaag aatcgtgctc ggtgcggttg cgatcttcgc
     2941 gctatccgtc gcctgcactc gccccgacgt cacggccgac acccagcctc tggctccgcc
     3001 agacaccctg acgccggtca cgacaacgtc aacccggcct cagaccacta ccaccgtgca
     3061 catcgatcgg gcgaagatct acccagtcga cccgatcacg ctggaagcag tcgccggagt
     3121 cgaaccgatt tcgatggggg atttcacttg ggggatttcg tccgaggacg gctcctggtt
     3181 ggcgatgacg gttggttatg acgatcggac ttcccaagag ttgcgactca tcgatgtcga
     3241 caactgggtg gtggcgaaca gctgggcctt cttagtcaac aacccgattc accttggaga
     3301 caacgggacc gtctacgtca ccgattattc gccaacaccc cacctcagtc agctgattcc
     3361 cggaaatgtg atcgcggatg tgatcgccga cctcccccca catttcattt ggtacgacgc
     3421 tcatttcacg gatcagacag gtctgatttt cgggctcgtg tctatcaacg atgacaacat
     3481 tggggaggcg gttctggtca ccgtggacct ggcgacatac gcggtcgacg tcatcccctt
     3541 gcccgaggtc gaaatcggga ccattgggtc aatggagatc gccgactttg gaccgaccaa
     3601 tttcgacatc tacccgcagg tggtctgggg cgacgatgga tcccgcgtgc tgatcgtcaa
     3661 caccaaccag gacgtggtag tcgaggtcga tgcggtctcc ggcgaggtcg acgagcacct
     3721 cttcggtctg aacacgctga actccactcc gacggaaacc gcaccgttcg agatcggcca
     3781 acggtcagca gtcatcggcg gcgccagtgg gcgagtgctc tatgtggcct caggggtcca
     3841 gaccttcgag ctaatcgacg aggacctaac gacgagattc gacccgttgg ggatcgaagc
     3901 aatcgacacc gagacctggg aggtcatcga ccgtctcgac gctccgatca ctgacgtcca
     3961 cctgtcttcg gacggcaacc gtctcctcgc caccggtcag tcgtatgacg acaatcccca
     4021 aagcccgcag tcgcgatctt cggggtttta cgtcatcgat gccctagatc tgagcgtgat
     4081 tgctcatcac ggaggcgaag agcccgatcg ctatttcgga gggttggcaa tgaatccggg
     4141 gctacctatc ggatatgtcc agacatggga gcatgcaacc agcatcgacg ttgtcgacct
     4201 cgacgttggg tcaattgtgg caacgaggag cggcggcgac atccaattct ttgctgaggc
     4261 aggggtactg gtcgacacct cgttcggaac ttcgtagaac cgcgtatcag gcacccctct
     4321 cggcgctctc ctccgccgac gtgaacaaat cgtccacgtt ggacacagag gaggaacaat
     4381 gtctacgagt gtcattcatc cgggtaccac ccggtcggcc ggaccctccg gtcgctttgt
     4441 agggttggcc gctgccgcac tggctgcagt catcgccctc ggtttcgtat tcgctgcaaa
     4501 tcagggaaac gaagccaccg agtcagtgac cgagtcacga gtcagccagg ttgagaagga
     4561 tgcctgggac tcgagggtcg acttcctgac ccagcagtac cacatgcggc aggcctcaca
     4621 gcccaccgtg aacatcgaga agtaccggct ggagatggca gggctcatcg gggcgatcac
     4681 gccctcgacc agccccgagg cctacaaggc tctgcaacag caggctggca aggctcaagt
     4741 caacccccgt attctggtga caatcccggc gccggtgttc agccctgagc tggcccgt
//
LOCUS       DBNHXU010000259         7234 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_875229, whole genome shotgun sequence.
ACCESSION   DBNHXU010000259 DBNHXU010000000
VERSION     DBNHXU010000259.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7234)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7234)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7234
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_875229"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="AC161X_06515"
     CDS             complement(<1..455)
                     /locus_tag="AC161X_06515"
                     /EC_number="3.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HIR4989241.1"
                     /translation="MKIVRVKAGDDISYGVADAEGVLVYRGSPFVAWEPTETVVPWDR
                     VQLLAPVIPTKLVCVGRNYVDHAGEMGEDLPDEPLIFLKPATAVIGPDASVVLPPSSN
                     DVHHEAELAAVINRVARNVRAEDASQYILGYTAANDVSARDLQTKDGQWA"
     gene            complement(469..978)
                     /locus_tag="AC161X_06520"
     CDS             complement(469..978)
                     /locus_tag="AC161X_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989242.1"
                     /translation="MGQQPNQEITEAEKPRATPETPPARSWHPTKPGLITSPDQVPKG
                     GRFGAAGPDAGWALRLLSLSELPDDDPNLRGVLAGLMTARAAALGRAPIKEDLEVALV
                     LCGYGFEAPPEVVATRERWKAAVPHEARPGQTAVAETDKELIVNKPEQVRWALTRAER
                     PQGEPGALS"
     gene            complement(978..1892)
                     /locus_tag="AC161X_06525"
     CDS             complement(978..1892)
                     /locus_tag="AC161X_06525"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007571304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004084 -
                     branched-chain-amino-acid:2-oxoglutarate transaminase
                     activity [Evidence IEA];
                     GO_process: GO:0009081 - branched-chain amino acid
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transaminase"
                     /protein_id="HIR4989243.1"
                     /translation="MEPSSKIWVDGEFVDWDDATVHFLAHGLQYGTGVFEGIRAYKTP
                     EGTAVFRLHEHMIRLVASARAYGIPMPWTVDQMDEACLELLRVNELESAYIRPLSFYG
                     AGAFGINPASSPVHTYMAAFFLGSYMGEEGIQKGITTMVSSWRRISHTSFIPTAKGSG
                     QYLNSVLAKQEAVAAGFDEAIMLNDGGSLSEGSGMNLFTVNGDVVCTPPVSSGILEGI
                     TRNSVIQLLKAEGVEVREVDLARGSLYTADEVFFTGTAAEVTPVREIDRRLVGNGEPG
                     PVTRKAQDLFSDAVAGKLSDYRDWLTFV"
     gene            1992..2450
                     /locus_tag="AC161X_06530"
     CDS             1992..2450
                     /locus_tag="AC161X_06530"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF016489.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006644 - phospholipid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A family protein"
                     /protein_id="HIR4989244.1"
                     /translation="MHRLVASFFGSGLILRRIRESDSGSGTVGALVALPFSLWIGTSL
                     GWPGQLFGAVLLTMAALWSTRPLVPEVGDAGWIVIDEAAGAFVATIGLLGWPAVIGFL
                     VFRVADIVKSRFTGVLQAERLPGAIGITADDVVAGLYGLAIGHLVAFALT"
     gene            2511..2951
                     /locus_tag="AC161X_06535"
     CDS             2511..2951
                     /locus_tag="AC161X_06535"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR4989245.1"
                     /translation="MDWHPKVIVVGVDGSETSHGAAEHAVAMARQWKGKLLVVTVVRT
                     PEGWWGIGGAPPPPEALSAALAEGQAQVLNELEETLDLEGVDYETVEELGDPSSRLLA
                     VAEANSADLIVIGRRGAGFAERVMMGSVADRLCHQSEVPVLVVP"
     gene            2994..3584
                     /gene="cofC"
                     /locus_tag="AC161X_06540"
     CDS             2994..3584
                     /gene="cofC"
                     /locus_tag="AC161X_06540"
                     /EC_number="2.7.7.68"
                     /inference="COORDINATES: protein motif:HMM:TIGR03552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043814 - phospholactate
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-phospho-L-lactate guanylyltransferase"
                     /protein_id="HIR4989246.1"
                     /translation="MPTVAILPVKPFRLGKGRLADSLSVEDRVALSEAFAVHTADLAA
                     AAGLIPLIVAGDEEVAEWALMRGIPTVEDPGDDLSAAAAAGVEWAKAAGSLWLVLHSD
                     LPLLELDELADLNDAMEQSGAVIAPSADGGTSALGGGEPIRFAYGPGSFHRHLVRMPE
                     AAVVARTGLLHDVDSPADLISARNHPRGAWLADVTR"
     gene            3581..4801
                     /locus_tag="AC161X_06545"
     CDS             3581..4801
                     /locus_tag="AC161X_06545"
                     /inference="COORDINATES: protein motif:HMM:NF025042.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008887 - glycerate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase type-2 family protein"
                     /protein_id="HIR4989247.1"
                     /translation="MSRFDPRVLANDPVRRTEVVGWLERGLLAVDPERLTREALDDSN
                     QQRPAVIIAIGKAAPAMARGAAECLEVIGGVCVSDHSEAVPPMIRAMAGNHPLPGAAS
                     FEAGKAVVDCVVNAPADARLLALVSGGGSAVCESPRPGISADYLSMATGALLKGGASI
                     GALNLVRRHLSSIKCGGLARAAGRPIETLVLSDVAGADPAIVASGPTIPTASDPAGAR
                     EVLVRHHIEVPDSVWEAMNRDAGPLELPTVTLLGDGRTAAQAVGEIQSGPYMVMPGWL
                     DGDIDTCLDHFLAGAGPGVTIAAGEPVVEVNGSGTGGRNTHAALLAARRLVGTNTLFT
                     AFATDGVDGLSGTAGAIVDGRTIERGGDPARALADFDSANYLATTGDLLICPPTGTNV
                     ADLWILRRQTPPAT"
     gene            4840..5544
                     /locus_tag="AC161X_06550"
     CDS             4840..5544
                     /locus_tag="AC161X_06550"
                     /inference="COORDINATES: protein motif:HMM:NF024950.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR4989248.1"
                     /translation="MEKTVVITGGTGGLGTALVRRLIFEDYRLVVTYLLPDEATRFEE
                     EFDIDEDRLLLLRVDATNAEAVNSLMKNVSEQWGSIHGLCSLVGGWAGGRDVEETDDV
                     RFERMIDLNLRSAFYAVRAVVPYLKEAGWGRIVLVGSKSAVDYPTGQAAFNLSKAGVV
                     ALAKSVAQELDGTGVTANVLMPSVIDTPATRQSLPYADYVTWPTPDEIAAVAHFLLSD
                     DSGVMNGASIPVYGRA"
     gene            complement(5529..6527)
                     /locus_tag="AC161X_06555"
     CDS             complement(5529..6527)
                     /locus_tag="AC161X_06555"
                     /inference="COORDINATES: protein motif:HMM:NF021599.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2332 domain-containing protein"
                     /protein_id="HIR4989249.1"
                     /translation="MWTSPEGVRAWGKHAHGSPLYAHLVEVLADDEELMRVLHRIEHL
                     PQVNLILGSVHYLLLRDPAHDLAAWFASVVEDPRPVNEVDPVFRSFLLEHEDEVVGLA
                     NSHYTQTNECRRCIALLPAVMTSGLDRFHLVEVGASAGLNLALDRYHYEFGDLSWGPN
                     SPVILDGDWRGRRVALRDIDVLSRTGLDLSPILPGDVDSQQWLDALIWPEQRERRARL
                     RAAMTMVSALGLEMVAGDALETLPAALEKLPAGEPAVVMNAFTFGQLTPEGRARIEEI
                     CDVERKSRPVYRVSLELLANADEWARVSVTDHGPLVQIGQAHPHGEWVELWDQARP"
     gene            complement(6527..>7234)
                     /locus_tag="AC161X_06560"
     CDS             complement(6527..>7234)
                     /locus_tag="AC161X_06560"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: protein motif:HMM:NF012304.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0043137 - DNA replication, removal of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H family protein"
                     /protein_id="HIR4989250.1"
                     /translation="PVLGVFLCDTCGRTFEVRQEVLDRYPGWVPRQCLDCKNGSGGEP
                     TSSKLGSGGPDMTPSRPNYGDGAGSPSRTKSKDLTTAEVLASFSDGPTTGVFTDGASE
                     GNPGPGGWGAVYVENDHIVDQAHGSEPHTTNNRMELAALIHGLGMLPNDSTMTLYTDS
                     QLIVNIVTKWADGWKAKGWTKKSSGPIANLELVKEAHALYKQRPGVRLEWIKAHNGYR
                     WNEYADALATAYRRQVF"
BASE COUNT         1292 a         2322 c         2286 g         1334 t
ORIGIN      
        1 gcccactgcc cgtccttggt ctgcagatcc cgcgccgaca cgtcgttcgc cgccgtgtag
       61 cccaggatgt attgcgaagc gtcctcggct cggacgttcc gagccaccct gttgatgacg
      121 gcagccaact cggcctcgtg atgcacatcg ttcgaggacg gaggcagcac caccgaagcg
      181 tcaggcccga tcactgcggt ggcgggcttc aggaagatga gtggctcgtc cggcaagtcc
      241 tcccccatct cgccggcgtg gtcgacgtag ttgcgcccga cgcagacgag cttggtcggg
      301 atcaccggtg ccagaagttg gactcgatcc caggggacca cagtctcggt cggctcccac
      361 gcaacaaatg ggctcccccg atagacgagc accccttcgg cgtcggcgac gccatacgaa
      421 atgtcatctc cagccttgac ccgaacaatt ttcatgagat gctggacgct agctgagggc
      481 gccgggctcg ccttgcggtc gctcggcgcg ggtcagtgcc caacgaacct gttcgggctt
      541 gttgacgatc aactctttgt cggtctcggc taccgccgtc tgaccgggcc tggcctcgtg
      601 ggggaccgcg gccttccagc gctctctcgt tgccactact tccggcggcg cctcgaagcc
      661 gtaaccacac aacacgagcg ccacctcgag gtcctccttg atcggagcac gtccgagggc
      721 agcggcccga gcagtcatca ggcccgcaag cacaccacgg agattgggat catcatcggg
      781 cagctctgac aacgagagga gacgcaaagc ccagccggca tcaggccccg cggcaccgaa
      841 gcgcccgccc ttcgggactt gatccggaga agtgatgagc ccaggcttgg ttggatgcca
      901 gcttcgggct ggaggagtct ccggggtggc tctgggcttt tcggcctcgg tgatctcctg
      961 gttcggctgt tgacccatca gacgaaggtc agccagtccc gatagtccga aagctttcct
     1021 gccaccgcat cagagaacag gtcctgtgcc ttgcgcgtca ccggccccgg ctcgccgttg
     1081 ccgaccaatc gcctgtcgat ttctcggacc ggggtgacct cggccgccgt cccggtgaaa
     1141 aacacctcat cggctgtata gaggctgccc cttgcgagat ccacctctct cacctcgact
     1201 ccctcggcct tgagcaattg aatgacgctg ttcctggtaa tcccctccaa gatccccgac
     1261 gagaccggtg gagtgcagac gacgtccccg ttcactgtga acaggttcat cccgctcccc
     1321 tccgagagcg accctccatc gttcagcatg atcgcttcgt caaagcctgc ggcgactgcc
     1381 tcctgcttgg cgagcacgga attgagatat tgcccgctgc ccttggccgt cggtatgaag
     1441 ctcgtgtgtg agatccggcg ccagctggag accatagtgg tgatcccctt ctggattccc
     1501 tcctcgccca tgtagctgcc aaggaagaag gcggccatat aggtatgcac cgggctcgac
     1561 gcgggattga tgccgaacgc cccggcgccg tagaacgaca gagggcggat gtaggcggac
     1621 tcgagctcgt tcacccggag caattcgagg cacgcctcat ccatctgatc gacggtccaa
     1681 ggcatcggga ttccgtaggc gcgagccgag gccaccaggc gaatcatgtg ttcatggagc
     1741 ctgaatacgg cggtcccctc cggcgtcttg taggcgcgaa tgccttcgaa gacacccgtt
     1801 ccgtattgaa gcccgtgggc gaggaaatga acggttgcgt cgtcccaatc gacgaactcc
     1861 ccgtccaccc atatcttgga tgacggttcc atagccatga ggctaactgc tgcgacggaa
     1921 agtgaaatcg ttgccggacc caagatgggt ggcggaggcg gctccacaac cggatctagg
     1981 ctcgaatgcg aatgcatcgc ctggtcgcct cctttttcgg ctccggtctg atcttgcgcc
     2041 ggattcggga atcggactcc gggtcgggga ccgttggagc cttggtcgcg ctccccttct
     2101 cgttgtggat cggcacgagc ttgggatggc cgggccagct gttcggagcc gtgctactga
     2161 ccatggctgc actctggtcg acccgccccc tggtgcccga ggtcggggac gcgggctgga
     2221 tcgtgatcga cgaggccgca ggagccttcg tcgccaccat cggcctcctc ggatggccgg
     2281 cggtcatcgg atttctcgtg ttccgtgtcg ccgacatcgt caagagtcgt tttacgggag
     2341 ttctccaggc cgagcgtctg cccggggcca ttgggatcac tgccgacgac gtggtggccg
     2401 gtctctatgg gctcgccata gggcatttgg tcgcatttgc ccttacttga ggacctcctc
     2461 ctcttcggag gtgagggcga atcgcctatg ttgaaggttg gaggtgaatg atggattggc
     2521 atcccaaggt gatcgtggtc ggagtcgatg ggtcagagac cagccatggc gcagctgagc
     2581 acgcggtcgc catggcgaga cagtggaaag ggaagctcct cgtggtgaca gtggtcagga
     2641 cgcctgaagg atggtggggc atcggtgggg cgccaccacc acctgaggcc ctctcggccg
     2701 cattggccga gggccaggca caggtgctca acgaattgga ggagaccctc gatcttgaag
     2761 gggtcgacta cgagacggtt gaagagctcg gtgacccctc ctcgcgcctc ctcgcggttg
     2821 cggaggccaa cagcgccgat ctgatcgtga tcggacgccg cggagcaggt ttcgccgaac
     2881 gggtgatgat gggatcggtc gctgacaggc tgtgtcacca atccgaagtc ccggtgctcg
     2941 tggtcccctg atcctgacag gagggagtgg gaactcgcct ctaccctcgg cgggtgccga
     3001 cagtcgccat cctcccggtg aagccttttc ggctcggcaa gggacgactc gctgactcac
     3061 tctcggttga agacagagtc gccctgagcg aggcattcgc ggtccacacc gccgatctcg
     3121 cagccgccgc gggtctgatc cccctcatcg tcgccggtga cgaggaggtc gccgaatggg
     3181 cactcatgcg cggtatccct acggtcgaag accccggaga tgacctgagc gcggcggccg
     3241 cggcgggggt cgagtgggcg aaggcggcgg ggtcgctctg gctggttctg cactctgatc
     3301 tcccactact ggaactcgac gagctggctg atctcaacga tgccatggag caatcagggg
     3361 cggtgatcgc cccttcggcc gacggcggga cttcggcatt agggggtggc gaaccgatcc
     3421 gattcgcata cggacctggg agtttccatc gacacctggt gaggatgccg gaagcggcgg
     3481 tggtcgcccg caccggcctg ctccacgatg tggattcacc ggcggacctc atctccgctc
     3541 gcaatcaccc ccgcggcgcc tggctggcgg acgtcacaag atgagccgat ttgatccccg
     3601 agtgttggca aacgatccgg ttcggcgcac agaggtggtc gggtggctgg agaggggttt
     3661 gctcgccgta gacccggagc gcctcactcg cgaggccctc gatgactcga accaacaacg
     3721 gcccgcggtg atcattgcca tcggcaaggc ggctccagcc atggcgcgcg gggcggcaga
     3781 gtgccttgag gtgatcggtg gagtctgcgt ctccgaccat tccgaggctg tcccaccgat
     3841 gatccgcgca atggccggca atcacccgct ccctggtgcg gcatcctttg aagccggcaa
     3901 ggcggtcgtc gattgcgtgg tcaacgcacc agccgatgcg cggctgttgg ccctggtctc
     3961 gggaggaggg tcagccgtct gcgagtcgcc tcggcccgga atctcagcgg actacctgag
     4021 tatggccacg ggtgccctgc tcaaaggagg cgccagtatc ggggcgctga atctggtgcg
     4081 acgacacctg tcctcgatca aatgtggcgg cttggcccga gcggcaggtc gcccgatcga
     4141 gaccttggtg ttgtctgacg ttgcgggggc cgacccagcg atcgtggctt cgggcccgac
     4201 catccccact gcgtccgatc cggcgggggc gagagaggtc ctggtccgtc accacatcga
     4261 ggtccccgac tcggtgtggg aggcgatgaa ccgggatgcc ggcccactcg aattgccgac
     4321 ggtcacactg ctcggcgatg gtcgcactgc agcgcaagcc gtgggcgaga tccagagcgg
     4381 gccatacatg gtgatgcccg gatggctgga tggggatatc gatacctgcc tcgaccactt
     4441 cctagcgggc gccggtcccg gtgtgacaat cgcagccgga gaaccagtgg tggaggtcaa
     4501 cggctccggg accgggggcc gcaacacgca cgccgccttg ctcgccgccc gacgcctggt
     4561 cgggaccaac accttgttca cggcattcgc caccgacggg gttgacggtc tctcggggac
     4621 cgccggggcc atcgtcgacg ggagaaccat cgaacgggga ggagacccgg cccgggcatt
     4681 ggccgacttc gactctgcca actatctggc gaccaccgga gatttgctga tctgcccccc
     4741 aaccgggacc aacgtcgccg atctctggat tctccggcga caaactccgc ccgcaacgta
     4801 gagttagcgg accgacaact tcagaaagca cttgaaagcg tggaaaagac agtcgtaatc
     4861 accggaggca ctggcggcct cgggaccgca ttggtccgtc gactcatctt cgaggactat
     4921 cggttggtag tcacttacct ccttcctgac gaggcgaccc gattcgagga agagttcgac
     4981 atcgacgagg accgcctgct tctcctccgc gtcgacgcca caaatgccga agcggtcaac
     5041 agcctgatga agaacgtgtc cgagcagtgg ggctcgatac atggcttgtg ctcgctggtc
     5101 ggtggttggg ccggaggccg tgatgtcgag gagactgacg atgtccgatt cgaaaggatg
     5161 atcgacctca atctccgttc ggccttctat gcagtgcgtg ccgtggtccc ctatctgaaa
     5221 gaggcgggat ggggacggat cgtcctggtc ggcagcaaga gcgcggtcga ttacccgact
     5281 ggccaagccg ccttcaacct ctcgaaagca ggagtggtcg cccttgccaa atcggtggca
     5341 caggagttgg acgggaccgg agtcaccgcc aacgtcctga tgccctcagt gatcgacaca
     5401 ccagccaccc gacagtcgct cccctacgcg gactatgtca cctggccgac tcctgatgag
     5461 atagctgccg tcgcccactt cctcctcagc gacgactccg gcgtgatgaa cggggcatcg
     5521 atccccgtct acggccgagc ctgatcccaa agctcgaccc attccccgtg gggatgggct
     5581 tggccgatct ggaccagagg cccgtggtcg gtcaccgaga ctcgtgccca ctcatccgcg
     5641 ttggccaaca gctccaatga gacccgatag accgggcgac tcttccgttc cacatcacag
     5701 atctcctcga tccgggctct cccctcgggc gtgagttgtc caaatgtgaa ggcattcatc
     5761 accactgcag gctcaccggc cggcaacttc tcgagcgcgg ccggcagagt ctccaatgca
     5821 tcgccggcca ccatttccaa ccccagcgcc gacaccatcg tcatcgcagc acgaaggcgc
     5881 gcccgccgtt ctcgctgctc gggccaaatc agggcgtcca gccactgctg tgagtcgaca
     5941 tccccgggca ggatcgggct caggtcgaga cctgtccgac tcaggacatc gatgtctcgc
     6001 agagcgaccc gcctcccacg ccagtcaccg tcgagaatca cgggtgaatt cgggccccaa
     6061 ctcaggtctc cgaactcgta gtgatatcga tccagggcaa gattgagccc ggcgctggcc
     6121 ccaacctcga ccaagtgaaa gcgatcgagg cccgatgtca tcaccgccgg caacagggcg
     6181 atgcatcgcc ggcattcgtt ggtctgggtg taatggctgt tggccaatcc aacgacctcg
     6241 tcttcatgct cgaggaggaa tgagcgaaac accgggtcga cctcattcac cggccgcggg
     6301 tcctcgacca cggacgcaaa ccaggccgcc agatcgtgag ccggatcccg aagcaatagg
     6361 taatggacgc tgcccaggat cagattcacc tgcggcaggt gctcgattcg atgcaggacg
     6421 cgcatcaact cctcatcgtc ggcaaggacc tccacgagat gggcgtagag cggcgaccca
     6481 tgagcgtgct taccccacgc tcgaaccccc tcagggctgg tccacatcaa aagacctggc
     6541 ggcgataggc ggtggcgagc gcatcggcat actcgttcca ccgatagccg ttgtgggcct
     6601 tgatccactc caggcgcacc ccaggccgct gcttgtagag ggcgtgcgct tctttgacca
     6661 gttccagatt ggcgatcggg ccactggact tcttggtcca ccccttggcc ttccaaccgt
     6721 cagcccactt ggtcacgatg ttcacgatga gctgtgagtc ggtgtacagg gtcatggttg
     6781 agtcgttcgg gagcatgccc agaccatgga tcagggcggc gagctccatg cggttgttgg
     6841 tggtgtgcgg ctccgagccg tgggcctggt cgacgatgtg gtcgttctcg acatagaccg
     6901 ctccccatcc ccccggcccg ggattgccct ccgacgcgcc gtcggtgaac accccggtag
     6961 tcgggccgtc actgaacgag gcgagaacct ccgccgtcgt caggtccttg ctcttggtcc
     7021 ggctgggcga gcccgcaccg tctccgtaat tgggacggga aggggtcata tccggacctc
     7081 cagatcccaa tttcgaggag gtcggctcac cacctgagcc gttcttgcag tcgaggcact
     7141 gacggggcac ccagcccgga tagcggtcga ggacctcttg acggacttcg aatgtgcgcc
     7201 cgcaggtgtc acagagaaac acccctagaa ccgg
//
LOCUS       DBNHXU010000260         8526 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1868466, whole genome shotgun sequence.
ACCESSION   DBNHXU010000260 DBNHXU010000000
VERSION     DBNHXU010000260.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8526)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8526)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8526
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1868466"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1701
                     /locus_tag="AC161X_06565"
     CDS             <1..1701
                     /locus_tag="AC161X_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012259099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0097054 - L-glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HIR4989251.1"
                     /translation="KNSAIKQVASGRFGVTPAYLDSADELQIKMAQGSKPGEGGQLPG
                     HKVSAEIARLRYTEPGVTLISPPPHHDIYSIEDLAELIYDLKAFKPTARVSVKLVSEP
                     GVGTIAVGVAKADADVIVLSGSEGGTGASPLVSIKHAGSPWELGLAEAHQVLTANGQR
                     SRVLLETDGGLRTGRDIVVAALLGAERFGFGTLPLLALGCKMVRQCHLNTCPVGIATQ
                     NEDLRAKFTGSADQVVLLFRRLAEEARRHIASLGARSIAELVGRADLLRPINPEHPLS
                     ADLSAMLVASKGGKKHEGYRKIGLSSLSVRLVADCRPAIADGRSVELAYPIRNVDRAI
                     GARLSGEITARHGSEGLPDGSIVVKLSGTAGQSFGAWLCRGVDMHIEGAANDYLGKGM
                     GGGLISVSPRRPGETVPHVAGNAVLYGATGGKVYLSGRVGQRFAVRNSGATAVIEGCS
                     DHGCEYMTGGKVLVLGEVGRNFAAGMTGGVAYVWDPALSLKAMLADTAPSAKRPSDTL
                     LDDIRGMIESHLERTASPVAAGILDGWSQEQGSFWVISASGDSRSIPILEQAEVPVAP
                     "
     gene            1771..3666
                     /locus_tag="AC161X_06570"
     CDS             1771..3666
                     /locus_tag="AC161X_06570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020677829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015079 - potassium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0071805 - potassium ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HIR4989252.1"
                     /translation="MTSASDHSRSPRYLLLLSVGALGVVYGDIGTSPLYAMREAFHAA
                     GDLTVTRDNVFGVLSLVFWALVIVVSIKYLIFVLRADNSGEGGIMALTALILPPNRRI
                     RVRGLHRVLILLGLFGTALLYGDGMITPAISVLSAVEGFEIVAPELASWVVPISIVIL
                     VGLFAIQKTGTGTVGAVFGPVMIVWFVTIGLLGAIQIFSDPGVLAAVNPAYAVRFIVA
                     APRVAFLALGAIFLVATGSEALYADMGHFGRRPIRLGWVFVVFPALILNYFGQGALIL
                     ANPAAIDNPFYRLAPGWALLPTVLLATAATVIASQALISGAYSLTMQAVQLGFLPRVD
                     IDHTSPREVGQVYVASINWLLMFACIGLVVAFRTSSNLAAAYGVAVTSTMVITTLLLY
                     RVSRERWGWTRLRAGSVMSVFLAVDLAFFAANIIKIPAGGWFPLVVGALIFVVMVTWR
                     RGRGDLSLEMRHGQVSIERFIASIAQHPQRRVPGTAVYLIREAGFAPPAMLANLRHNE
                     ILHETMVLLTVRAANVAKVPQARRATVHDLGDSFYQVILDYGFMEEPDVPRALASIVD
                     PEFGFDPSDASYFLGKESVVVVPGGGIRSWRSRLFSFLHRNSSSAAVQFSLPSDQVLE
                     IGTQVRM"
     gene            3688..4152
                     /locus_tag="AC161X_06575"
     CDS             3688..4152
                     /locus_tag="AC161X_06575"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018280456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
                     /protein_id="HIR4989253.1"
                     /translation="MVVEIPKGSRNKYEMDHETGEIFLDRMLFTATRYPADYGFIPDT
                     LAEDGDPLDILALVSEPTFPGCRIRVRPVGVFLMEDQGHADHKVLSVPLGDPLWPDAE
                     NLDDVPAHVLSEVEHFFQVYKELEKKKTAVIGWRTREEALTVIERALAAYPH"
     gene            4235..6001
                     /locus_tag="AC161X_06580"
     CDS             4235..6001
                     /locus_tag="AC161X_06580"
                     /inference="COORDINATES: protein motif:HMM:NF012706.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4989254.1"
                     /translation="MKRVLVLVTAMVLAVAIVPIASAQDEGDEITLRVGITQDWDGLN
                     PGLNYSVPSYDVWNLQYMALTDKAAADFATIPSLAESWDVSDDKLTYTYHLREGLVWS
                     DGEPLTAEDVVWNINTSRDQGWDNMIATVANLDATAVDDLTVQVTTAVPDPKLPTLDM
                     YLVPPQAWEPIATDYDAAMQYGGASEDKVGSGPFVVTDFTKGQSVTMEVNPSFFGWNG
                     EEPPYDRIVFRLFENPDAMVAALQQNELDVIHGFPAGSWDTLDADPNIEVVAGQQGGF
                     DEIAINGGALPEGQPHPALLDLKVREAISHGIDRAAVIEDLWFGQAAPLETLSPSPDP
                     KWIPEIPEADVFDYDPDQSNQILDDAGYADSDGDGIREMPDGTPIVLRHAVNTDGDLA
                     PTIGDLFVGWMEAIGIGVEISSYDQDALYDVIATGQYDTFFWGWTPFVDPDPMLSYFT
                     AAEVGNYNDANWSDPRYDELYLEQNQELDPERRVEIVHEMLTLFHDAAVYIPMYVSPD
                     LQAYRTDTFEGWVKQPAEIGAVIFSNTSPSYVLLSPISAEAGDSGSNNLLVIGGVVVG
                     VVVIGGIFLAMRKRSTTADDRE"
     gene            6061..6996
                     /locus_tag="AC161X_06585"
     CDS             6061..6996
                     /locus_tag="AC161X_06585"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989255.1"
                     /translation="MLGALLTLVFVLVFNFFLFRVVQTDPIGSLFRGRNVPAETLENL
                     RIAFGLDKPIGTQFLFYLKETAQLNLGISYQTRQAVWDQIVVAIPPTVALVGVSAVFS
                     AVIGTIGGVIAAWRRNTWKDHSITSGTMVFYAMPDFWLGMILLLIFAVVLGWFPVGGI
                     TDPGTNETGLALLLDQLHHMFLPALTLTLAYLGEYTLVTRSSLLDVMGEDYLTVARAK
                     GLRDVVVRNRHAVPNALLPVVTLTAINFGFVLSGAIAVEAVFSWPGLGKATADAIRGP
                     DLPMLQGLFLLFSAAVIFFNLVADLTYSWLDPRVD"
     gene            6999..7889
                     /locus_tag="AC161X_06590"
     CDS             6999..7889
                     /locus_tag="AC161X_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006240728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989256.1"
                     /translation="MASIAMRRRRTTAGRVWKQFRSSRQGMIGLAVLMLFVLMALAAP
                     LLADVRELNPVFARAQGNPQWAPPSVFPPLGTDYIRRSVWAQFVWGSRVSLFVGLAAT
                     VLTILIGSVVGIVSGFVGGRLDAVMMRITEWFLVIPFLPLAIVLASVLGRNVWNIIFV
                     IGITSWPSTARLVRAQVLTVKQRLFVDRARSLGASGTHIVGHHILPNVSGLILANATL
                     AVPISILTETTLSFLGLGDPTAASWGKTLEEAFVNGAVVRNAWWYFLPAGLGILAVVL
                     AFTLIGRTLEEIANPRLSGR"
     gene            7889..>8526
                     /locus_tag="AC161X_06595"
     CDS             7889..>8526
                     /locus_tag="AC161X_06595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019764433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989257.1"
                     /translation="MSEPMLSVRDLTITYHTSGGPVPAVRGVSFELEKGGSIGLAGES
                     GCGKSTLANSLLRLLPAETEVTGEITLNGQDVLTLSPGGLRAVRWTSGAIVFQGALHA
                     LNPVQTVGDQISEPMLIHETASSGTVARERTVELLRRVGLPAARADSYPHELSGGQRQ
                     RVLIAMALACDPLLLIADEPTTALDLMVQAQVLALLDDLKKERDLTLLFITH"
BASE COUNT         1455 a         2632 c         2666 g         1773 t
ORIGIN      
        1 aagaattcgg cgatcaaaca ggtggcatcc gggcggttcg gggtgacccc cgcctatctc
       61 gattccgctg atgagcttca gatcaagatg gcgcaaggct ccaaacccgg agaggggggc
      121 cagcttccag ggcacaaagt ctcggctgag attgcccgtc tccgctacac cgagcccggg
      181 gtgactctca tctcgccccc accccaccac gacatctatt cgatcgagga cctggccgag
      241 ttgatatatg acctcaaggc cttcaaaccg acagcccggg tttcggtcaa gctcgtcagc
      301 gagccgggag tcgggacgat cgctgtcgga gtcgccaagg ctgatgccga cgtgattgtc
      361 ctttccgggt ccgagggcgg aactggcgcc tccccgctgg tttcaatcaa gcatgcaggc
      421 tcgccgtggg agctgggcct tgccgaagcg catcaggtgc tgacagccaa cgggcagagg
      481 tcacgggtac tgcttgagac cgatggtggg ctgaggaccg gccgggacat cgtggtcgcc
      541 gccttgctcg gagcggagcg gttcggcttt ggcacgcttc ccctccttgc cctgggctgc
      601 aagatggtca ggcagtgtca cctcaacacc tgcccggtcg gcatcgctac ccagaacgag
      661 gacctacggg ccaagttcac cggatcggcc gaccaggtgg tcttgctctt ccgacgcctt
      721 gccgaggagg cccgtcgtca cattgcttcg cttggggcac ggtcgattgc agagctggtc
      781 ggtcgcgccg accttctccg gccgatcaac cctgagcatc cgctgtccgc cgatctctcg
      841 gcgatgttgg tggcctcaaa gggaggcaag aagcacgagg gttaccgcaa gatcggcctg
      901 tccagcctgt cggtgagact ggttgccgat tgcaggccag cgattgccga tgggaggtcg
      961 gtggagctgg cctacccaat ccgaaatgtc gatcgtgcca tcggtgctcg tctctccggg
     1021 gagatcaccg ccagacacgg gtccgagggc cttcccgatg ggagcatcgt cgtcaagttg
     1081 tcaggaaccg ccggccagag ctttggcgca tggctgtgtc gcggtgtcga tatgcacatc
     1141 gaaggggccg ccaacgacta cctgggcaaa gggatgggtg gtggactgat ctctgtttcg
     1201 cctcgtcggc ccggggagac cgtgccccac gtcgcgggca acgccgtgct gtatggggcc
     1261 accgggggca aggtctacct ctcgggaagg gtgggccagc gctttgcggt gcgcaattcc
     1321 ggcgcaacgg cagtgatcga gggatgctcg gaccacgggt gtgagtacat gaccggtggc
     1381 aaggtcctcg tgttgggtga ggtcggccgg aacttcgcag ccggcatgac cggcggcgtc
     1441 gcctatgtgt gggacccggc tctcagcctc aaggcgatgc tcgccgacac cgcaccttcg
     1501 gccaagcgac cgtccgacac ccttctcgac gacatccggg gcatgatcga gtcccatctg
     1561 gagcgcaccg ccagcccggt tgcagctgga atcctcgatg gctggtcaca ggagcagggc
     1621 tccttttggg tgatttcggc aagtggtgac agtcgctcga taccgatcct ggagcaggca
     1681 gaggtcccgg tcgctccctg accccttctc tggtggccac gggtacgcct gatacggcaa
     1741 tccgcaccga tacagtgccc cgttaccctt atgacatcgg catctgacca ctcccggtcg
     1801 ccccgttacc tcctcctgct ttcagttggc gctcttggcg tggtctatgg ggacatcggc
     1861 accagccctc tctacgccat gcgtgaggct ttccatgctg ccggggatct cacagtcacc
     1921 cgggacaacg tgtttggagt tctctctctg gtgttctggg ccctggtgat cgtggtgtcg
     1981 atcaagtatt tgatattcgt gctccgagcc gacaactcgg gcgagggcgg gatcatggcc
     2041 ctgaccgcat tgatcctgcc tccgaatcga cgcatccggg tgcgagggct acatcgcgtc
     2101 ctcatcctgc tcggtctgtt cggcaccgcc ctgctctatg gagacggaat gatcacccct
     2161 gcgatctccg tgctctctgc cgtggaggga ttcgagatag tcgcacctga actggcctcc
     2221 tgggtcgtac cgatctcgat cgtcatcctg gttggcctat tcgccatcca gaagacgggg
     2281 accgggaccg tgggggcggt tttcggtccg gtgatgatcg tgtggtttgt cacgatcggc
     2341 ctcctcggcg ccattcaaat cttctccgat cccggcgtgc tggcggcggt gaatcccgca
     2401 tacgcggttc ggttcatcgt tgcggcgccc agggttgcgt tcctcgcttt aggggccatc
     2461 ttcttggtag ccacgggtag tgaggctctc tatgccgaca tggggcactt cgggcggcga
     2521 ccgatcaggc tcggatgggt gttcgtcgtc tttccggcct tgatattgaa ctatttcggt
     2581 caaggagcgc tgatcctggc caacccggcg gcgatcgaca acccgttcta tcggctggcg
     2641 cccggatggg ccctccttcc cacggtcctg ctcgccaccg ccgccacggt cattgcctcc
     2701 caggctctca tctcaggcgc ctactcgttg acgatgcagg ccgtgcagct cggcttcctc
     2761 ccccgagtgg atatcgacca cacctcgcct cgagaagtcg ggcaggtgta cgtggcttcg
     2821 atcaactggc tgctgatgtt cgcctgcatc gggctggtgg tggcgttccg cacttcgagc
     2881 aaccttgcag cggcatatgg ggtggcggtt acttcgacga tggtgatcac cacgctcctg
     2941 ctctaccggg tctctcgtga gcggtgggga tggacccggt tgagagcggg ttcggtcatg
     3001 tcggtgttcc tcgccgtgga ccttgccttt tttgccgcca acatcatcaa gatcccggcc
     3061 gggggctggt tcccgctggt ggtgggggca ctcatcttcg tggtcatggt gacctggcgt
     3121 cgcggccggg gcgacctctc gctcgagatg cgacatggtc aggtgtcgat cgagcgtttc
     3181 atcgcttcca tcgcccagca ccctcagcgt cgggtgccag gaaccgcggt ctatttgatc
     3241 cgagaggcag gattcgcccc gccggcgatg ctggccaatc tcagacacaa cgagatcctt
     3301 cacgagacca tggtcttgct cacggttcgg gcggccaacg tcgcgaaggt cccccaggcg
     3361 agacgagcca cggtccacga tcttggagac agcttctatc aggtgattct ggactacgga
     3421 ttcatggagg agcccgatgt cccccgggcc ctggccagca tcgtcgaccc ggagttcggg
     3481 ttcgaccctt ccgacgccag ctacttcctg gggaaagagt cggtggtggt cgtgcctggc
     3541 ggcgggattc gcagctggcg atcccgattg ttcagtttcc tgcatcgtaa ctcgtctagt
     3601 gcggcagttc agttcagctt gccttctgat caggttttgg agatcggcac ccaagtccgc
     3661 atgtgaggta gccccatgga agtgacgatg gtcgtagaga ttcccaaagg gagtcgcaac
     3721 aagtacgaga tggatcacga gacgggggag atcttcctcg accggatgtt gttcaccgcc
     3781 acccggtatc cggctgacta cgggttcatc cccgacaccc tcgccgagga tggcgatccg
     3841 ctcgacatcc tggcactggt aagcgagccc acttttccgg gctgtcgcat ccgggtccgc
     3901 ccggttgggg tcttcctgat ggaggaccaa ggccacgccg accacaaggt tctcagcgtt
     3961 ccgttggggg atccgctgtg gcccgacgca gagaacctcg acgacgtgcc ggcgcatgtg
     4021 ttgagcgagg tcgagcactt cttccaggtc tataaggaac tggagaagaa gaagactgcg
     4081 gtgatcggct ggcgaacccg ggaagaagca ctgactgtca tcgagcgggc gctggctgcc
     4141 tatccccact gaccagtagc accgttcgag gcgacacagc gtcgaaaccg gatatagatt
     4201 ccccagggcc acactgaacg gagagtcagc cttcatgaaa cgtgtccttg ttttggttac
     4261 cgccatggtg ttggcggtcg cgatcgttcc tattgcctcg gcgcaagacg agggggatga
     4321 gatcaccctg cgcgtcggta tcacccaaga ctgggatggt ctcaaccccg gtctcaacta
     4381 ctcggtgccc agctatgacg tgtggaacct tcagtacatg gcgctcaccg acaaggcggc
     4441 cgccgacttc gcaacgattc cctcgctcgc agagtcgtgg gatgtctcag acgacaagct
     4501 gacctacacc taccatctgc gtgaggggct ggtttggtct gacggtgagc cgctcaccgc
     4561 cgaggacgtg gtgtggaata tcaacacgtc ccgcgaccag ggctgggaca acatgatcgc
     4621 gacggttgcc aatctggatg cgaccgcggt cgacgacctc accgttcaag tcacgacggc
     4681 ggttcccgac cccaagttgc ccaccttgga catgtacctg gttcccccac aggcctggga
     4741 gccgatcgcc accgactatg acgctgccat gcagtacggc ggggcgtccg aagacaaggt
     4801 cggcagcggc ccatttgtcg tcaccgactt caccaaaggc cagtcggtga caatggaagt
     4861 caacccgagc tttttcggct ggaacggcga ggagccgccc tacgaccgaa tcgtgttccg
     4921 tctttttgag aaccccgatg cgatggtggc cgccctccaa cagaatgagt tggatgtcat
     4981 ccacggtttc ccagccggtt cttgggacac gctcgacgcc gatcccaaca tcgaggttgt
     5041 ggccggacag cagggtggat tcgacgagat cgcgatcaac ggcggggcac tgcccgaagg
     5101 tcagccccat cctgccctgc tcgacctcaa ggttcgcgaa gcgatctcac acggcattga
     5161 ccgggcggca gtgatcgagg acctctggtt cggccaagcc gctccgttgg aaaccttgag
     5221 cccttccccc gaccccaaat ggatacccga gatccccgag gctgatgtat tcgactacga
     5281 cccagaccag tcgaatcaga ttctggatga tgccgggtat gcggacagcg atggtgatgg
     5341 gatcagggag atgcccgacg ggaccccgat cgtccttcgc catgcggtga acacagatgg
     5401 tgatctggcg cccacgattg gcgatctctt cgtcggttgg atggaggcca ttggcattgg
     5461 cgtcgagatt tcgtcctacg accaggacgc cctgtatgac gtgatcgcca cgggtcagta
     5521 cgacactttc ttctggggct ggacaccctt cgtcgacccc gacccgatgc tgtcctactt
     5581 cacggcagcc gaggtcggca actacaacga cgccaactgg agcgacccca ggtacgacga
     5641 gctgtatctc gagcagaatc aggagttgga ccccgagcga cgggtagaga tcgtccacga
     5701 gatgttgacc ctgttccacg atgccgcggt ctacatcccg atgtacgtca gcccggatct
     5761 ccaggcctat cggaccgaca ccttcgaggg ttgggtcaag cagccagcgg agatcggggc
     5821 tgtcatcttc tccaacacct cgcccagcta tgtcctcctg agtcccatct cagctgaagc
     5881 aggcgacagc ggatccaaca atctgttggt gattggcggg gtcgtggtcg gagtagtcgt
     5941 gatcggcggc atcttcctgg ccatgagaaa gcgctcgact acggcagacg accgggagta
     6001 aacccgctgg ccagcgaaca gaaacgcggc gaggggcgag gcgggctcgt cctgcgcaag
     6061 gtgttgggcg ccctgctcac tctggttttc gtcctggtgt tcaacttctt cctgtttcgc
     6121 gtcgtccaga ccgaccccat tggctcgctg tttcgggggc gcaacgtgcc ggccgagacc
     6181 ctggagaacc ttcgaatcgc cttcggattg gacaagccga tcgggactca gtttctcttc
     6241 tatctgaagg aaacggcgca gctcaacctg ggcatttcat atcagacccg gcaggcggta
     6301 tgggatcaga ttgtggtcgc cataccgccg acagtggctt tggtgggcgt gtcggcggtc
     6361 ttctcagcgg tgatcggcac catcggtggt gtcatcgccg cctggcgccg aaacacctgg
     6421 aaggaccaca gcatcacttc ggggaccatg gtcttctacg caatgcccga cttctggttg
     6481 ggcatgatcc tcctcctgat ctttgcagtg gtgttgggat ggtttccggt gggggggatc
     6541 accgaccctg gcaccaacga gaccggcctg gctctcctcc tcgatcaact ccaccacatg
     6601 ttcttacccg cgctcacatt gaccctggct tacttggggg agtacaccct ggtcaccagg
     6661 agctcactgc tcgacgtgat gggcgaggac tatctaaccg tcgctcgggc caagggtttg
     6721 agggatgtgg tggtcaggaa ccgtcatgcg gttcccaacg ccttgctccc ggtggtgacc
     6781 ttgaccgcga tcaactttgg attcgtgctc tccggggcga tcgccgtcga ggccgtcttc
     6841 tcctggcccg gcctgggcaa ggcgaccgcg gatgccatcc gcggaccgga ccttcccatg
     6901 cttcaggggc tgttcctgtt gttcagtgcg gcggtgatct tcttcaacct ggtggctgat
     6961 ctcacataca gctggctcga tccgagggtg gactgaccat ggccagcatt gcaatgcgac
     7021 gtcgtcggac cacggcggga cgggtgtgga agcagttccg gagctcgcgt cagggaatga
     7081 tcgggctcgc cgtactgatg ttgttcgttt tgatggctct cgccgcgcct ttgctcgccg
     7141 acgtcaggga gctcaacccg gtgttcgcaa gggctcaagg caatccgcag tgggcaccac
     7201 cgagcgtatt cccacctttg ggcactgact acatccgacg ttcggtttgg gcacagttcg
     7261 tatggggctc gcgggttagc ctgttcgtcg ggctcgccgc caccgtgctc acgattctca
     7321 ttggctcggt ggttgggatc gtctccgggt tcgtcggtgg ccgcctcgac gcggtcatga
     7381 tgcggatcac cgagtggttc ctggtcatcc cgttccttcc tcttgcgatc gtcctcgcat
     7441 cggttctggg tcggaatgta tggaacatca tctttgtgat cgggatcacc tcctggcctt
     7501 cgactgcgcg gttggtccgg gctcaggtgc tcaccgtcaa acagaggctc ttcgtcgatc
     7561 gtgcccgctc gctaggagca agcggaaccc acatcgtggg ccaccacatc ctccccaacg
     7621 tgagtgggtt gatcctggca aacgcaacac tggcagtccc catctcgatc ctgaccgaga
     7681 ccaccttgtc cttcctcggg ctaggtgacc ccacggcggc ctcgtggggt aagactctgg
     7741 aggaggcctt cgtcaatgga gcggtggtcc gcaacgcttg gtggtacttc ctgccggccg
     7801 gcctcgggat cctggccgtt gtcctggctt tcacgttgat cgggcgcacc ctggaggaga
     7861 tcgccaatcc gagactgagt ggccgctgat gagtgagcca atgctctccg ttcgcgacct
     7921 caccatcacc tatcacacgt cgggcggccc ggtgcccgcg gtgcgggggg tcagttttga
     7981 gctcgagaag ggtggatcga tcgggctggc cggcgagtcg ggttgcggca agtcgactct
     8041 cgccaactcc ctgctccggc tactacctgc agagaccgag gtaaccgggg agataacact
     8101 caacggccag gacgtgctca cactgtcacc gggcggactg agggcggtgc gctggacttc
     8161 gggagccatc gtgtttcagg gcgctttgca cgctttgaac ccggttcaaa cagtgggcga
     8221 tcagatctcc gagcccatgc tcatccacga gaccgcgagc tcaggcaccg tggcccggga
     8281 gcggaccgtc gagctgctgc gccgggtggg tcttccagcg gcccgggccg actcctatcc
     8341 ccacgagttg tccgggggtc agcggcagcg agtcctcatc gcgatggcgt tggcctgtga
     8401 tccactgctg ctgatcgctg acgagccgac cacggcgctc gacctcatgg tgcaggccca
     8461 ggttctggcg cttctggacg atctcaagaa agagcgcgac ctcactctgc ttttcatcac
     8521 gcatga
//
LOCUS       DBNHXU010000261         6800 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3654295, whole genome shotgun sequence.
ACCESSION   DBNHXU010000261 DBNHXU010000000
VERSION     DBNHXU010000261.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6800)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6800)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6800
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3654295"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1542)
                     /locus_tag="AC161X_06600"
     CDS             complement(130..1542)
                     /locus_tag="AC161X_06600"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019735491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045240 - alpha-ketoacid dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HIR4989258.1"
                     /translation="MPTTVTMPQLGETVTEGTILRWAKKVGDSIVEDEVLVEISTDKV
                     DTEVPSPASGTVLEILVAEGDTVGVGTALVVIGEAGEAPVSPPRSGSDIGGRTPEGGG
                     GQPDEAPSPPPAPVEPEPAPVEAAKEPTNEEVDAFFAEPDETEKQPNLLSPVVRKLIR
                     ENEIDVSRLSGSGQGGRITRKDVESYLAGAPAAASASEPEPEPEPVETSAAPSPVAPI
                     EPTPTPAPVVVGEGSEEVELDRLRVRTAENLIRAKQTAAHVWTSVEVDYERVERVRQA
                     HKDQFKSAEGFSLTYLPFISRATIDALKAFPIVNSSFYLEEKKSVLHKKVDLGIAIDL
                     NQSGLVVARVRNADGLRLVGIARSIRELADRGREGKLGVDDLTGSTFTITNPGPFGSF
                     MSAPIINVPNVAILSTETVSKRAAVITTDDGQDMIAIRHIGYLGLSWDHRAFDGSTAV
                     LFLQRIKQNLETWDWEQELA"
     gene            complement(1555..2922)
                     /gene="lpdA"
                     /locus_tag="AC161X_06605"
     CDS             complement(1555..2922)
                     /gene="lpdA"
                     /locus_tag="AC161X_06605"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     (NADH) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HIR4989259.1"
                     /translation="MYDIVIVGGGPGGYASALYAHNFGLSVAMIDKERPGGTCLIRGC
                     IPAKQWLHVADVFNTVANAAEFGVITEQPSLDWTAALARKNQTVEGLVRGLAGLLKQK
                     GVDVLTGFGKLAGPGKVEVASEGGTTVVEGRTVILASGSYARTIPGYDIDGRQIVTSD
                     HALDWERRPDSVAIIGGGVIGCEFASFLVDVGAEVHVFEVMDQILPGSDPDAARQLQR
                     QLQKRGVKFHIGVTVGPPVLGSSVRVPFGEESVEVDVVLVSVGRGPNTDGVGLETTSA
                     VLDRGFVKVDLSTMQTDQPGLYAVGDIVAGTAQLAHVGFAEGIAAVTHIATGKTQPVN
                     YQAIPRVTYTHPEVAEVGLSEADAVAQGLEVSAAKHAFNGIGRAIIIGDNQGFVKVVS
                     AKDGPIIGATVVGPHAGELIHELMYSVGWEALPAEAAALIHAHPTLSEAVGETLMSAA
                     GRSLH"
     gene            3078..3671
                     /locus_tag="AC161X_06610"
     CDS             3078..3671
                     /locus_tag="AC161X_06610"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR4989260.1"
                     /translation="MSDRIELETADGEHLEARWDHASDPIGATVFCHPHPLDRGSMNA
                     PLMIGVTLRLVELGFSVLRFNFRGTGTSTGSHDHGTAEMLDVAAAAATARSTGLPLSL
                     AGWSFGAGVALNWLAGEQSPPPYSGIAPPPENLPGSLPPGQKRVIVGTREQVIDTQAL
                     LAYAARLSIDVVLTPGDHFFHGRGKKIGDLVAQGLAG"
     gene            complement(3668..3907)
                     /locus_tag="AC161X_06615"
     CDS             complement(3668..3907)
                     /locus_tag="AC161X_06615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989261.1"
                     /translation="MSRLSRFPHYRYIGVRDTMKFYDCDEAGEFDELKSRVDSEDLVG
                     RNGLQAFAPDTPEEAINRGYSPATRPPPHQVESID"
     gene            complement(4251..4700)
                     /locus_tag="AC161X_06620"
     CDS             complement(4251..4700)
                     /locus_tag="AC161X_06620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989262.1"
                     /translation="MSGPVVFAFVALLVGIFLIVVALVVWQEAKRRPSYEPLTYVVDD
                     AVKHVDTGLAAEGNTNLTRADIRRILDWEVFYLQGLAQEDRSNPVETVAGGHEASIDY
                     IIDQIATKHGVSYAREDVENVLRMEADYLYQIGAIGEPVEFEGDEEE"
     gene            complement(4734..6527)
                     /locus_tag="AC161X_06625"
     CDS             complement(4734..6527)
                     /locus_tag="AC161X_06625"
                     /EC_number="1.3.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017594433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HIR4989263.1"
                     /translation="MSHYRSNLRDIEFNLFEVLRIQDHLGAGEWSDLDLNIATDVLHA
                     IERLAREEFAAAYVDQDRIPLELIDGSVVLPESVKVGVKAYKDGGWDRFGARVELGGM
                     PVPPTLLWATQEMLLASNTTVAWYAGGSLFAGVLFEEGTEEQKALAQLWMERQWSGSM
                     ILTEPDAGSDVGASVTKAIDMGDGTWHLEGVKRFITGGEHDATENIIHLVLARPEGAI
                     AGSKGLSLFVVPKYLVNEDGSLGERNGVVATHIEKKMGLKGSTTCEMTFGAETPAIGT
                     LVGDVHDGIRQMFRVIEGARMMIGTKAMGTLSTGYLNALEYAKERHQGADLTKFGDKT
                     APRVPIISHPDVRRMLMNLKAHSEGLRALVMYTAWVQDQSRLQPDDDRWERRSDFLLP
                     LVKGYGSETAYHQLADALQVLGGSGYTQDYPLEQYIRDTKIDTIYEGTTGIQALDLFF
                     RKISRDQGNTVAALASEITEFVKGGGTDDPIAPERELLGVMLDGAQTQLGSMVEHLLD
                     SFTGDSAEIYKVGLHTNSLLASFAEVVIAWQLLRQAEIAFVKVDEDPFYVGKVASARW
                     FVRLAAPQVGARRDAAVSEDGAVMGLPPEAF"
     gene            6611..>6800
                     /locus_tag="AC161X_06630"
     CDS             6611..>6800
                     /locus_tag="AC161X_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012655.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR4989264.1"
                     /translation="MRYKAGLETNDRILDATRSLIAGKGLEGTTIKAICDEAGVLPGS
                     FYNLFDSKEQAVLTVVREA"
BASE COUNT         1252 a         2188 c         2039 g         1321 t
ORIGIN      
        1 acgcctccga atagggaagg cgtcctaacc atcggaccct aagggtccga tggttgtccc
       61 aagtctcagg tctcaagctt ctggcctgcg acttgcgatt tgggacctgg gacagggagc
      121 gcagcgaact caggcaagct cctgctccca gtcccaggtc tccagatttt gcttgatcct
      181 ttgcaggaag agcaccgcag tcgagccatc aaacgcccgg tgatcccagg acagtcccaa
      241 atagccgatg tggcgtatgg cgatcatgtc ctggccgtcg tcggtggtta tcaccgccgc
      301 ccgcttcgac acagtctcgg tggagagaat cgccacattc ggcacgttga tgatcggcgc
      361 cgacatgaag gacccgaacg ggcccgggtt ggtgatggtg aaggtggatc cggtcaggtc
      421 atcaacgccc aacttccctt cacggccccg atccgcaagt tcgcggatgg agcgagcgat
      481 tcccaccaga cggagcccgt cagcgttgcg tactcgcgcg actactagcc cactctggtt
      541 gaggtcgatg gcgataccga ggtccacctt tttgtggagc accgacttct tctcctccag
      601 gtagaacgag ctgttgacga tcgggaatgc cttgagggca tcgatagtcg cccgggagat
      661 gaagggcaga taagtcagcg agaaaccctc ggcgctcttg aactgatcct tgtgcgcctg
      721 gcgaactcgc tccacccgct cgtagtcgac ctcgaccgag gtccagacgt gagcagcggt
      781 ttgcttggcc cggatcaaat tctccgcggt cctcaccctg agccgatcca actcgacctc
      841 ctcggagccc tcaccaacaa cgacgggggc aggggtgggc gtgggctcga tcggcgcaac
      901 ggggctgggt gcagcacttg tctccacagg ctctggctct ggctccggct ccgaggccga
      961 ggccgctgcg ggggcgccgg caaggtagct ctcgacgtct tttcgggtga ttcggcctcc
     1021 ctggccgctg cccgacaacc gggagacatc gatctcgttc tcgcggatca gcttgcgcac
     1081 caccggcgac agcagattcg gctgcttctc ggtctcgtcc ggctcggcga agaaggcgtc
     1141 gacctcctcg ttggtgggct ctttggcagc ttccaccggt gccggttcag gctcgactgg
     1201 cgcaggaggc ggagaagggg cctcgtcagg ctgcccccct cccccctccg gggttctccc
     1261 cccaatgtcg ctgccgctcc tggggggaga gacgggcgct tcgccggctt cgccaatcac
     1321 gaccagagcg gtaccgaccc cgacggtatc cccctcggcc acgagtatct cgaggacagt
     1381 gcctgacgcc ggggacggga cctcggtgtc caccttgtcg gtcgatatct cgacgagtac
     1441 ctcatcctcg acgatcgaat caccaacctt cttggcccat cgcaggatgg ttccctcggt
     1501 gacggtctcc ccgagttggg gcatcgttac ggtggtcggc aaagctggct cctctcagtg
     1561 aagactgcga ccggcggcgg acatcaaagt ctcgccgacc gcctctgaca acgtcgggtg
     1621 ggcgtggatc aacgccgctg cctcggcagg caaggcttcc catcccactg aatacatcaa
     1681 ctcgtgaatc agttccccgg catgcggacc gaccacggtc gcgccgatga tggggccgtc
     1741 tttggccgac accaccttca cgaagccctg gttgtcgcca atgatgatcg ctcgcccgat
     1801 cccgttgaag gcgtgtttgg ccgcagacac ttccaggccc tgggccacag cgtcggcctc
     1861 ggataagcca acttccgcca cctcggggtg ggtgtaggtg acgcggggaa ttgcctgata
     1921 gttgacaggt tgcgtctttc cggtggcgat gtgggtcacc gcggcgatgc cctcagcgaa
     1981 accgacatga gccaactgcg cggtaccggc gacgatgtcg ccaacggcgt agagacctgg
     2041 ctggtcggtc tgcatcgtgg acaaatcgac cttcacgaat ccccgatcga gcaccgccga
     2101 agtcgtttcc aggccgacgc catctgtatt cggcccccta cccacagaaa ccaggaccac
     2161 gtcgacttcg accgattcct cgccgaacgg cacccttacc gatgatccga gcaccggcgg
     2221 tccgaccgta accccgatgt ggaacttcac acccctcttc tgcaattggc gctgcagttg
     2281 gcgggctgca tcgggatccg acccgggaag gatctggtcc atgacctcaa acacgtggac
     2341 ttcggcgccg acatcgacga gaaaggaagc gaactcacag ccgatgaccc ctccgccgat
     2401 gatggcgacc gagtcaggtc gccgttccca gtcgagggcg tggtccgatg tgacgatctg
     2461 cctgccatcg atgtcatagc cgggaatggt tcgggcataa gagccgcttg ccaggatgac
     2521 tgtgcggccc tcgaccactg tggtgccccc ttccgaggcg acctcgacct tgcccggacc
     2581 ggccaacttc ccgaatccag tcaagacgtc gacgcccttc tgcttgagaa gcccggcgag
     2641 gccgcggacc aggccctcca ctgtttggtt cttccgggcc agagcggccg tccagtcgag
     2701 gcttggttgc tcggttatga ccccgaattc agcggcgttg gcaacggtat tgaagacatc
     2761 ggcaacgtgg agccactgct tggcggggat gcagccacgg atgagacagg tgccgcccgg
     2821 acgctctttg tcgatcatgg cgaccgagag cccgaagttg tgggcgtaca gagcggaggc
     2881 gtatccacct ggaccgccgc cgactatgac gatgtcgtac aacactgacc tttcggtggg
     2941 actgtcctcc cgagcctagt tgtgccaggg gttaccctcc ccaaccgaat gccaacccgc
     3001 tggctcctca tcgcatcggc cgttctcgcc ttcgtaatca tcggtgcggc cgccatctgg
     3061 ttcttcatcg tgctgggatg agcgatcgaa tcgagttgga aaccgcggac ggcgagcacc
     3121 tcgaagcccg ttgggaccac gcctccgatc cgatcggagc aactgtcttc tgccatcccc
     3181 atccgttgga ccgggggtca atgaacgcgc ccttgatgat cggggtcacc ctccgtctcg
     3241 tcgaactggg attctccgtg cttcgattca acttccgggg caccgggacc agcaccggca
     3301 gccatgacca cggcactgcg gagatgttgg atgttgcggc ggcggcagca acagcccgca
     3361 gcaccggcct gccgttgagc ctggctggat ggtcgtttgg cgcgggcgtc gccctcaatt
     3421 ggttagccgg cgagcagtcg cctcccccat attcggggat cgccccaccg ccggagaacc
     3481 tgccgggctc actgccccca ggccagaagc gcgtcatcgt tgggaccaga gaacaggtga
     3541 tcgacactca agccctcctg gcctacgcag cgcgcctttc gatcgacgtc gtcctgacgc
     3601 caggtgacca tttctttcat ggccggggca agaagatcgg cgacctggtg gcccaaggat
     3661 tggccggtta gtcgatcgac tcgacctggt gtggtggcgg ccttgttgcg ggtgaatagc
     3721 cccgattgat ggcttcctcg ggggtgtcgg gagcgaaagc ctggaggcca ttccgtccaa
     3781 ccagatcctc tgaatcgacc ctcgacttca actcgtcgaa ctcgccagct tcgtcacagt
     3841 catagaactt catcgtgtcc cggacgccga tgtaccggta gtggggaaag cgactgagac
     3901 gactcattcc tcttctgcca acacagggct ctcatcttcg aaactgggag tcggtgtccc
     3961 ccggcctgtc tgcacttcga tgccacagtt gggacaagtg gcgaccggga tctctcccac
     4021 gatgaaggtg aaagcgcagt cgggacaact gatcctgggc gatggccggt cgggctgagg
     4081 cggaggcggt gccgcgggag cgccagtttg cgccgtcgga taagggccgg ccccaggcgg
     4141 ggcggccccg gcctgattgc gaacgacatt caccgagcca cagctgggac acgagaaccg
     4201 gccggcgccg gcgacgtcga attgagtccg gcaagcgccg cacctaacga tcattcctct
     4261 tcatctcctt cgaattcgac tggctctccg atggcgccga tctgatacaa gtagtcggct
     4321 tccatgcgga gcacattctc cacgtcttcc cgagcgtagg agacaccgtg tttggtggct
     4381 atctggtcga tgatgtagtc gatcgacgct tcatgccctc ccgcgacggt ctccacgggg
     4441 ttggaccgat cctcctgtgc cagaccctgc agatagaaga cctcccaatc caggattcgc
     4501 ctgatgtcgg cacgggtgag attggtgttg ccctctgcgg ccaaaccggt atccacgtgt
     4561 ttcaccgcgt catccaccac ataggtcagc ggctcgtagc ttggtcgtcg ttttgcctcc
     4621 tgccacacca ccagagccac cacgatcagg aagatgccaa cgagtagggc aacgaaggca
     4681 aatacaacag gacccgacat tacggtcaac ggtaaacggt gacgggtgaa cgactagaac
     4741 gcttcgggcg gcagccccat cactgccccg tcttcgctca cggccgcgtc ccgcctcgct
     4801 cccacctggg gggcagcaag acgaacgaac cagcgagccg atgccacctt gcccacgtag
     4861 aagggatcct catcgacctt cacaaacgcg atctcggcct gccgcaggag ttgccaggcg
     4921 atgacgacct ccgcaaagga cgcaaggaga gagttggtat gcaacccaac cttgtatatc
     4981 tccgcggagt cgccggtgaa cgaatcgagg agatgctcga ccatcgatcc caactgtgtc
     5041 tgagccccgt cgagcatcac acccaacaac tcacgctcgg gggcgattgg atcgtcggtg
     5101 ccgccgccct tcacgaactc tgttatctcg gaggcaagtg ctgccaccgt gttgccctgg
     5161 tcgcgcgaga tcttccggaa gaagagatcc aatgcctgga taccggtggt cccctcgtag
     5221 atcgtgtcga tcttggtgtc acggatgtac tgctccagcg ggtagtcctg ggtgtagccc
     5281 gagccgccga gcacctgcag ggcgtcagcg agttggtggt aggccgtctc tgacccgtag
     5341 cccttgacca ggggcagaag gaagtcagag cgtcgctccc agcggtcgtc gtcgggttga
     5401 agccgtgatt gatcctggac ccaggcggtg tacatcacga gggcgcgcag cccttctgaa
     5461 tgcgccttga ggttcatcag catgcgtctc acgtcggggt ggctgatgat cgggacccga
     5521 ggcgcagtct tgtccccgaa cttggtgaga tcagcaccct gatggcgctc cttggcgtac
     5581 tcgagggcgt tcaagtaccc ggtgctcagg gtccccatcg ccttggttcc gatcatcatt
     5641 cgagcccctt cgatgacccg aaacatctgc cggatcccat cgtggacatc cccgaccaag
     5701 gtcccgatgg ctggcgtctc ggcgccgaag gtcatctcgc aggtcgtgga gcctttcagg
     5761 cccatcttct tctcgatatg agttgcgacg accccgttgc gctcgccgag gcttccgtct
     5821 tcgttgacca gatacttggg aacgacgaaa agggaaaggc ctttgctccc agcgatggct
     5881 ccttccggac gggccagaac gaggtgaatg atgttctccg ttgcgtcgtg ctcgccgccg
     5941 gtgatgaatc gcttcacgcc ttccaggtgc caggtgccgt cacccatgtc gatcgccttg
     6001 gtcacactgg cgcccacatc ggagccggcg tcgggctcgg tgagaatcat gctccctgac
     6061 cactgccgct ccatccagag ctgcgccagc gctttctgtt cctcggtccc ctcctcgaac
     6121 aacacgcccg caaacagcga tcctccggcg taccaggcga cagttgtgtt cgaagcgaga
     6181 agcatttcct gtgtcgccca cagaagcgtc gggggcaccg gcatcccgcc aagctcgacc
     6241 cgggccccaa agcggtccca tccgccatcc ttgtaagcct tgaccccgac tttcaccgat
     6301 tcggggagga cgacactccc gtctatcaac tcgagtggta tccggtcctg gtccacatat
     6361 gcggcggcaa attcctcacg ggccagtcgc tcgatggcgt ggaggacgtc cgtcgcgatg
     6421 ttcaggtcga ggtctgacca ttctcccgct ccgagatggt cctggatgcg aagcacctcg
     6481 aagagattga actcgatgtc ccgcagattc gacctgtagt gactcacccg actatctgct
     6541 cccagtattt gagtgataac tctaatttgt atgaacgctc aatgtaagtc ggagagctac
     6601 cgtttcggcc atgcggtaca aggcgggcct cgagaccaac gatcggatac tcgacgccac
     6661 cagatcgttg attgcgggca aaggcctcga agggacgacg atcaaggcga tctgcgacga
     6721 ggccggcgtc ctacccggca gcttctacaa cctgttcgat tcgaaggagc aggcggttct
     6781 gactgtcgtc cgcgaggcga
//
LOCUS       DBNHXU010000262         3292 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1869134, whole genome shotgun sequence.
ACCESSION   DBNHXU010000262 DBNHXU010000000
VERSION     DBNHXU010000262.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3292)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3292)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3292
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1869134"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            60..350
                     /locus_tag="AC161X_06635"
     CDS             60..350
                     /locus_tag="AC161X_06635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HIR4989265.1"
                     /translation="MTEQGPYPEQSQATTALVLGILGLVICGILGPFAWSMGNKEMQA
                     IDAGRRPPENRGTANAAKILGIVSTVLLAIGIVVIILLVIGTISLAVFSEAS"
     gene            358..639
                     /locus_tag="AC161X_06640"
     CDS             358..639
                     /locus_tag="AC161X_06640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011211922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4190 domain-containing protein"
                     /protein_id="HIR4989266.1"
                     /translation="MTQSPYPEPSQATTALVIGILGIVLCQILAPFAWSMGNKEMQAI
                     DAGRRPPENRGTANAARILGIVGTVLLGIAIIGVILFFTVFSIASVSSS"
     gene            685..1434
                     /locus_tag="AC161X_06645"
     CDS             685..1434
                     /locus_tag="AC161X_06645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989267.1"
                     /translation="MGQFSASLKVPGDTSSLPATVDIDDGRLRVASGDHEIGNWALDD
                     INLTPGPEGVHVSAEGEELILQFSESAAFEAAAGMNSKPSVVSLPKPFKLPTLSRSPK
                     PSSANGTATRPEGAPAPVVAPAASAAPIASSTKVEQKAPKEPKQPGAFDRGLGKAEKM
                     FGKWLPSWVFTRGGSLVVVAALVVAILAPSLVSTLLLVAGLVLLVLGGVTMLDVVLAA
                     RLLKAKVAPTQVLIGGGVLVVIGILFGMLAR"
     gene            complement(1434..1781)
                     /gene="rplQ"
                     /locus_tag="AC161X_06650"
                     /pseudo
     CDS             complement(1434..1781)
                     /gene="rplQ"
                     /locus_tag="AC161X_06650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009948672.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
     gene            complement(1781..2722)
                     /locus_tag="AC161X_06655"
     CDS             complement(1781..2722)
                     /locus_tag="AC161X_06655"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016444112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HIR4989268.1"
                     /translation="MIVQRPQIEEESISDTRSKIVVEPLEPGFGYTLGNTLRRTLLSR
                     IPGAAVTSIRIEGVEHEFSTIPGVVEDVVDIILNIKRVVVRMAGTDTEVIHLTAKGAG
                     EVKAGDIRLPAGVEIVNPEMHIATMSASGRLEMELTVAPGVGYRSADSNKGSVSTIGV
                     IPVDSIFSPVRKVSYRVENTQVGQMTNFDRLVLDVETNGAVDPGEVVSSAGKTLRELM
                     GLFADIGEGQGLELGDVQTEHAGFPEKELPIEALDLSERPRNCLRRAQIKTVGELIER
                     TSDDLLNITNFGQKSLEEVTAKLDELGLSLADATGGS"
     gene            complement(2735..>3292)
                     /gene="rpsD"
                     /locus_tag="AC161X_06660"
     CDS             complement(2735..>3292)
                     /gene="rpsD"
                     /locus_tag="AC161X_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011719170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HIR4989269.1"
                     /translation="TQLCQSKRCPVDRRPYPPGEHGRGRIRESDYLIQLREKQKLRSM
                     YGVLERQFRRYYEEAARRPGITGENLLRLLEQRLDNVVWRSGLAATRPQARQLVNHGH
                     FRVNGRKVDIASYQVRPGDVVTIKDASRDSIVIQHAVDTQGREAPAWLSVDLGERKVA
                     VTDQPRRDQIDTDINEQLIVELYSK"
BASE COUNT          602 a         1030 c         1010 g          650 t
ORIGIN      
        1 gctaccgccg ccgggacttt ggccagcact gcccccgatg atcgtgcgat aatcgaatca
       61 tgactgaaca gggcccttac ccagaacaga gtcaggccac caccgcatta gtgctcggaa
      121 tcctcggact cgtcatctgc gggatcctcg gtcccttcgc ctggtcgatg ggtaacaagg
      181 aaatgcaggc catcgacgcc ggacggcgcc cacccgaaaa cagaggaacg gccaatgcag
      241 ccaagatcct cgggattgtg agcaccgtat tgctcgctat aggcatcgtc gtcatcattc
      301 tccttgtcat cggaaccatc tccctcgcgg tcttctcaga ggcctcatga aagaatcatg
      361 actcagagcc catacccgga accaagccag gcaactaccg ccctcgtcat tggaatcctc
      421 ggaatagtcc tttgccagat cctcgctccg ttcgcctggt cgatgggcaa caaagagatg
      481 caggccatcg atgcggggag gcgcccaccg gagaaccggg gcaccgcaaa cgccgcaaga
      541 atcctgggga tcgtcggaac cgttcttctg gggattgcca tcatcggcgt catacttttt
      601 ttcactgttt tctccatcgc gtccgtgtcc tcttcctgag gttttccgcg caagtcgggc
      661 cgaaacctgc cgataaatgg ccctatgggt caattcagtg cttccctgaa ggtcccgggt
      721 gacaccagct cgctgcccgc caccgtcgac atcgacgatg ggcgcctgcg ggtcgcatcc
      781 ggtgatcacg aaatcggcaa ctgggcgttg gacgacatca acctcacccc aggtcccgag
      841 ggcgtccacg tttccgcaga aggcgaggag ctgatcctcc aattcagcga gagcgccgct
      901 ttcgaggcgg ccgcgggcat gaacagcaag ccgtccgttg tgtcgttgcc caagcctttc
      961 aagctgccca ccctgtccag gtctccgaag ccctcttcgg ccaacggcac ggcaacccgt
     1021 ccggaaggcg caccggcgcc cgtcgttgcg ccggcggcct cggccgcccc gattgcttca
     1081 tcgacgaaag tcgagcagaa agcacccaag gagcccaagc agccaggggc gttcgatcga
     1141 ggccttggta aggctgagaa gatgtttggg aagtggctac cgagctgggt gttcaccaga
     1201 ggcggatcgc tggtggtcgt tgccgcgttg gtagtggcca tcctcgcacc gtccctggtc
     1261 tccaccctgc tcctcgtcgc cggactggtc ctgctggtgc tcggtggagt gaccatgctg
     1321 gacgtggtct tggccgcccg actgctgaag gcgaaggttg ccccgactca ggttctgatc
     1381 ggcggcggcg ttctagtggt catcggcatc cttttcggga tgctcgcccg ctaaacgagc
     1441 tcgattatcg cctcttcggc gccgtctcca cgacgggggc cgatcttggt gatccgggtg
     1501 tagccaccag ctcgatccgc ataacgcgga gcgatgtcgt cgaacaatct cgacatggct
     1561 tgccggctgc gaattgtccg cagcacagtc cgctttgagt gaaggtcgcc ttttcgggcc
     1621 gtggtgatca gcttctcggc gagtggcctg accgccttcg ctctgggcag ggtcgtggtc
     1681 accttttcat gcatgaacaa gtcagtcgcc agattggcga ggagtttgcg ctcatgggag
     1741 ggcgagcctc cgagccgggc acccttgcgg ggacggggca tcaggaaccg ccggtggcgt
     1801 ctgccaggct cagcccgagc tcgtcgagct tggcggtgac ttcctcgagg ctcttctgac
     1861 cgaagttggt gatgttcagc aggtcatccg acgtccgctc gatcagctcg ccaacggtct
     1921 tgatctgggc acgacgcaga cagttgcggg gacgctcgga gagatccagt gcctcaatgg
     1981 gcagttcctt ctccgggaaa ccggcatgct cggtctggac gtcacccaac tcgagaccct
     2041 ggccctcacc gatgtcggcg aagaggccca tcagctcccg caaggtcttg cctgccgacg
     2101 agacaacctc gccggggtcg acggcgccgt tggtctcaac atcgagcacc agccggtcga
     2161 agttggtcat ctgccccacc tgggtgttct ccacccgata ggagaccttc cggaccggag
     2221 agaaaatcga atcgaccgga atgacaccga tcgtggagac cgagcccttg ttggaatccg
     2281 cagagcggta gccgacgcct ggggcgacgg tcagctccat ctcgaggcgc ccgctggcgc
     2341 tcatggtcgc aatgtgcatc tcggggttga cgatctcgac tcctgcgggg agccggatgt
     2401 ctccggcctt gacttcacct gcacccttgg cggtgaggtg tatgacctcg gtgtcggtgc
     2461 cggccatacg gaccacaacc cgcttgatgt tgaggatgat gtcgacgaca tcctccacaa
     2521 ccccagggat cgtggagaac tcgtgctcga ctccttcgat ccggatcgag gtgactgctg
     2581 cacctgggat acgggaaagc agggtgcggc gaagggtgtt gcccagtgtg tagccgaagc
     2641 ccggctccaa cggctcgacc acgatcttgg atctggtgtc tgagatcgat tcctcttcga
     2701 tctgggggcg ctgaacgatc aacaaagaat tacctcactt cgagtagagc tcgacgatca
     2761 actgttcatt tatgtccgtg tcgatctggt cccgtcgggg ctgatccgtc acggccacct
     2821 tccgctcacc gaggtcgaca gagagccagg ccggagcctc gcggccctgg gtgtccacgg
     2881 catgctggat gacgatgctg tcccggctcg cgtccttgat ggtgacgacg tcaccgggcc
     2941 ggacttgata ggacgctata tcgaccttcc tcccgttgac ccggaaatgc ccgtgattca
     3001 cgagctgccg ggcctgggga cgagttgctg cgagaccgga gcgccagacg acattgtcga
     3061 gacgctgctc caacaggcgg agcaggttct cgccggtgat gccgggccga cgagcggcct
     3121 cctcgtagta acggcggaat tgacgctcga gaacgccata catagaacgc agcttctgct
     3181 tctcgcggag ctggatcaga tagtcgctct cacggatgcg gccacggccg tgctccccag
     3241 gtggataggg gcgacgatcg acggggcaac gcttcgactg gcaaagctgg gt
//
LOCUS       DBNHXU010000263         1896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3655042, whole genome shotgun sequence.
ACCESSION   DBNHXU010000263 DBNHXU010000000
VERSION     DBNHXU010000263.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1896
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3655042"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..811)
                     /locus_tag="AC161X_06665"
     CDS             complement(<1..811)
                     /locus_tag="AC161X_06665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018605219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR4989270.1"
                     /translation="MDTNPVIEMMGRRLRLAVIGGGPGSFVGATHRIAATLDDRFELV
                     AGAISSEPERSRAAGVAIGLDADRSYGDGLEMLRVEAARDDGPDAVAIMTPNDTHHLF
                     SAAALSLGFDVICDKPITNTLDDALDLVSKVRETSLIFCLTHNYTGYPMVRQARAMVA
                     DGQLGEIRLVQVEYVQGGKAGEKDPDSKHGVPWRYDPVRGGPSLVMGDIGSHAHNLVR
                     FVSGLEVSEVSAEIGAIVPNRQVHDYGAAMLRFDNGARGSLWVTQAAAGVEN"
     gene            complement(820..>1896)
                     /locus_tag="AC161X_06670"
     CDS             complement(820..>1896)
                     /locus_tag="AC161X_06670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989271.1"
                     /translation="AYIIIIAGGLLITARLRLLAMAVTFWATLALGLGLLAASGHCFS
                     ASWSLQPVCGANFWWVVMGSPELLIFLFFMITDPKTIPARPTGRVAFAICLAVVCTLL
                     MAPQVTEFGTKVGLLAGLVVMSPLRLVFDRVFQEEPKSKRSLSPALVNRLTSSNGSDA
                     RPRLVFVRGTIVGAATMAIAVGIVAAGAPAREAALAEVTTEGTVFIPEVDPTSIPLVT
                     EGSEVASLKTDISTQEMAVVLAENLVIEGQALKTRDSSLLPRAVGGDRLVATLAMIND
                     SVATGEIVVPEYQFESLHAEVAEAKGGQGASIAFAASGVVDEITYTALGVEQARSSSP
                     FETTFVLSQVAGDRWLILEELPPS"
BASE COUNT          366 a          607 c          602 g          321 t
ORIGIN      
        1 aattctcgac tcccgccgct gcctgggtaa cccacaagct gccccgggcg ccgttgtcga
       61 agcgcaacat ggcggcgccg tagtcgtgaa cctggcggtt gggaacgatg gccccgatct
      121 cggcagagac ctccgagacc tcgagccccg acacgaaccg gacgaggttg tgggcatggc
      181 taccgatgtc acccatcacc agcgatgggc cgccccgaac cgggtcatag cgccagggca
      241 ccccatgctt ggagtcgggg tctttctcgc cggcctttcc gccctggaca tactccacct
      301 gcaccagtcg gatctccccg agctgaccgt cggccaccat ggctcgagcc tgtcgaacca
      361 tcggataccc cgtgtagttg tgggtgagac agaagatcag acttgtctct cgaaccttgg
      421 acaccagatc gagagcatcg tcgagggtgt tcgtgatcgg tttgtcgcag atcacatcga
      481 atccgagact cagggcagcc gcggagaaca agtggtgggt gtcgttgggg gtcatgatgg
      541 cgaccgcatc ggggccgtcg tcgcgcgccg cttcgactcg cagcatttcg agcccatccc
      601 cgtaggaacg gtcggcgtcg agcccgattg cgacgccggc ggcccgcgag cgttccggct
      661 cggaggatat tgcaccggct accaactcga aacggtcgtc gagggtcgcc gcgattcggt
      721 gggtcgcacc aacgaagctc cccggcccac cgccgatcac agccagccgc agcctgcgac
      781 ccatcatttc gatcactgga ttcgtgtcca tttggccttt caagaaggcg gcagctcctc
      841 gagaatgagc caacggtccc cggcaacctg gctcaacacg aatgtggtct caaacggcga
      901 actggacctc gcctgctcga ctccgagggc ggtgtaggtg atctcatcga cgacaccact
      961 ggctgcgaac gcgatactcg ccccctgacc tcctttggcc tcggcgactt cggcgtggag
     1021 ggattcgaac tgatactcgg gcacgacgat ctcgccggta gccaccgaat cattgatcat
     1081 ggccagggtc gcgaccaatc gatcgccgcc gaccgcccgg ggtagaaggc tcgagtcccg
     1141 tgtcttcaat gcctgccctt cgatcaccag gttctcggca agaaccacgg ccatctcctg
     1201 ggtgctgatg tcggtcttca aggaggcaac ctcggagccc tcggtgacga gagggatcga
     1261 agtcgggtcg acctctggaa tgaacacggt tccctcggtc gtgacttcgg caagcgccgc
     1321 ctcgcgggcg ggggcgccgg cagcgacgat cccgacggcg attgccatgg tggcggcgcc
     1381 gacgatcgtc ccccgcacga acaccaagcg gggtcgtgca tcactgccgt tggagctggt
     1441 caggcggttt acgagagccg gtgacagaga gcgcttgctc ttgggctcct cctggaagac
     1501 ccgatcgaac accagtcgga gcgggctcat caccaccaat cccgccagga ggccgacttt
     1561 ggttccgaac tcagtgactt gtggggccat gagaagtgtg cacacgacag cgaggcagat
     1621 ggcgaatgcg acgcggccgg tcggccgcgc cgggattgtc ttggggtcgg tgatcatgaa
     1681 gaagagaaag atgagaagct cgggagaccc catgaccacc caccagaagt tggctccgca
     1741 gaccggctgc agcgaccatg aggcgctgaa gcaatgaccc gaggcggcga gcagccctaa
     1801 cccgagcgcc aaagttgccc agaaggtcac cgccattgcg agaaggcgga gccgggcggt
     1861 gatcagcagc ccgcccgcaa tgatgatgat gtaggc
//
LOCUS       DBNHXU010000264         2066 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2862978, whole genome shotgun sequence.
ACCESSION   DBNHXU010000264 DBNHXU010000000
VERSION     DBNHXU010000264.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2066)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2066
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2862978"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            301..870
                     /locus_tag="AC161X_06675"
     CDS             301..870
                     /locus_tag="AC161X_06675"
                     /inference="COORDINATES: protein motif:HMM:NF016959.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009987 - cellular process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HIR4989272.1"
                     /translation="MSDTQPTFKTVRRGYDPGQVQSHLGLVSGQIQSLTDQVRSLGSE
                     VLQMRQERDAALQQGTAASAASYEQASARVAELMMTLDQDVARIRGEAEAEAEEILSH
                     ARSEAYQIRSEADEMVEAAERSVEDADRSVTDVKLRRDEVLEELRDTLSRVLGTIAGL
                     ATSIDDEHGGQEPDGSPVVLPEVLPERPA"
     gene            923..993
                     /locus_tag="AC161X_06680"
     tRNA            923..993
                     /locus_tag="AC161X_06680"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:955..957,aa:Gln,seq:ctg)
     gene            complement(1198..1365)
                     /locus_tag="AC161X_06685"
     CDS             complement(1198..1365)
                     /locus_tag="AC161X_06685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989273.1"
                     /translation="MSVSVLYMSMSLDGYIAGLDDEPGTPGGDGFERLHEWFGFGRTG
                     RPLGDLGPLVS"
     gene            complement(1398..1883)
                     /locus_tag="AC161X_06690"
     CDS             complement(1398..1883)
                     /locus_tag="AC161X_06690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1579 family protein"
                     /protein_id="HIR4989274.1"
                     /translation="MSEVTEETPGPVLPVPSPLLRGLEPFVGTWDFKGHLVGSGDEAI
                     TARSTFAWLPGGFFMQQRITIDFLGMSIESLELIGYDAGIDGLTSSVFSNLAPDALPY
                     FWNVSGKNVEVKVNYGLLHATFTGEFSEDGNQYSGGWRPSPGADPAVNVAYDLSGVRV
                     G"
     gene            complement(1900..2046)
                     /locus_tag="AC161X_06695"
     CDS             complement(1900..2046)
                     /locus_tag="AC161X_06695"
                     /inference="COORDINATES: protein motif:HMM:NF044751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic-ring hydroxylase C-terminal
                     domain-containing protein"
                     /protein_id="HIR4989275.1"
                     /translation="MWELPVVGEVPAPQAVLIRPDGHVAWVGDVDDRTLVGVLSEWFG
                     TSSE"
BASE COUNT          399 a          693 c          625 g          349 t
ORIGIN      
        1 cgtcgatcgc ggcgtcaacc acgcgcccga cgtcggcctt cgacaggccc gtccgcttcg
       61 ctacttcggc gaccaagcca cccttgttca tggtcgtccc tcccggtcga tcggggtcct
      121 cgatgcatcg ccaccgtagt cgcccacgtg catcgcggtc aaaccatgca cccggaaaga
      181 ggcttgcggc gcaaactgga acgatggtcg agagccgcgg cctaggtcgt aaggcctagc
      241 cgaggtgccg accatcgggc tctggcggaa cccgcagcat cgctctacgc ttcgtccgtg
      301 atgagtgaca cgcaaccgac cttcaagacg gtgaggcgcg gctacgatcc ggggcaggtg
      361 cagtcccacc tgggtctggt gagcgggcag atccagagcc tgacggacca ggttcgttcg
      421 ctgggatccg aggtgctgca gatgcgccag gaacgagacg ccgcgctcca gcaaggaacg
      481 gccgccagcg cagcctccta tgaacaagct tcagcccgcg tggcggagct gatgatgacc
      541 ttggatcaag atgtcgcgag gatcagaggc gaggcagaag ccgaggccga ggagatcctg
      601 tcgcacgccc ggagcgaggc ttaccagatc cgcagcgagg ccgacgagat ggtggaggct
      661 gccgagcggt cggttgagga cgccgaccgg tccgtcaccg acgtgaagct gcgtcgagac
      721 gaggtgctcg aggaactgcg ggacacgctc agcagggtcc tgggcacgat cgccgggctc
      781 gccacttcga tcgacgacga gcacggcggc caagaaccgg atgggtcgcc tgtcgtgttg
      841 cctgaagtcc tgccggaacg ccccgcgtag ctcaacactc gggcgccgca gtcgcagctg
      901 ctaccctccc aaggtcacac ggtggcccgt ggtgtaacgg tagcacgact gattctggat
      961 cagttagtct tggttcgaat ccaggcgggc cagcgacctc aggacgccgt ctccatccga
     1021 aacgccggac cttccgaacg gcatcgcgga agggtcggtc tcgatctggt cgagagttca
     1081 gcgccggacg ctgcagcgga agtggcatcc ggagtgtcaa tcacgcgcac gaatggtcga
     1141 cctgctccac tgtgcgacgg cccgccacga tggcaccgaa cccgcccatc tcctcgatca
     1201 actcactaac gggcccagat cgccgagcgg gcgacccgtc cggccaaagc cgaaccactc
     1261 gtgcagccgc tcgaagccgt caccacccgg ggtccccggt tcgtcgtcca ggcccgcgat
     1321 gtacccgtcg agcgacatcg acatgtagag aacggacact gacaacctct atcctcctgg
     1381 tctcgcccgc ttcggaccta tccaacacgc accccgctca agtcataagc aacattgact
     1441 gcagggtctg cgccggggct tggtcgccag ccacctgaat actggtttcc atcctcgctg
     1501 aactcccctg tgaacgtggc gtggagcagc ccgtagttga cctttacctc tacgttcttg
     1561 ccgctcacat tccagaagta gggcagcgca tcgggtgcga ggttggagaa gaccgatgac
     1621 gtcaatccgt cgatgccagc gtcgtacccg atgagttcga ggctctcgat cgacatccca
     1681 aggaagtcga tcgtgatcct ctgctgcatg aagaaccctc cgggtagcca tgcaaacgtc
     1741 gatctagccg tgatggcctc gtctccggac ccaactaggt ggcctttgaa gtcccacgtt
     1801 ccaacgaacg gctccaaacc cctcaacagc gggcttggaa ccggcagcac cggccctggt
     1861 gtttcctcag tcacctctga catggctctt cctctctcat cattcgctgg acgtgccgaa
     1921 ccactcggag agcactccca caagcgtgcg atcatcgaca tcaccaaccc acgcgacgtg
     1981 cccgtcgggt cggatgagta cggcttgggg cgccgggacc tctccgacaa cggggagctc
     2041 ccacaccccg tcgtaggtgg caccca
//
LOCUS       DBNHXU010000265         2321 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2663745, whole genome shotgun sequence.
ACCESSION   DBNHXU010000265 DBNHXU010000000
VERSION     DBNHXU010000265.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2321)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2321
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2663745"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1696)
                     /locus_tag="AC161X_06700"
     CDS             complement(176..1696)
                     /locus_tag="AC161X_06700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989276.1"
                     /translation="MSRDIVTELRRYSELLENSGGPLTLDEIMHHTGTIQEVPDQRDI
                     DRGHGHRWRPLIVAAALALMAGITLLIDVLRSDAPVVDNPPTTSVPGSTSTSSLPRAG
                     FEEGSLETPIGTVEWWYVNGSAATVPEVGDVFQTATGYGVISSSLEGPSYWTSLDGKS
                     WEPGSLAGLGDPAFVAPFGLIEDEYWVGTDDGLWKTPDGESWEQMPSLPQVDGDPGGV
                     WSTNGELWTVDWNPPSLWRLDGSEWVTVDVGDVGAQGGRDPNYSPGPIEWGENLIVPW
                     FFYDEGVLLSLIDPSGAATVVRPPWSSENIATEMAPEPTIVVDGSRIVAFVRGAVPGT
                     SCTESFAGPAGPCPTLEAWTSSDGVAWEGPVRMETMERDGYPGTGGFVSLVDDRWRAD
                     VVHAQADGHEVEVMFSDDGLTWFSSGIPLQGSFLRTVNLGAGEVVYGDAPWSEMFFST
                     DMRQWSRVDTSEVGILAGDEEEQRFSWGSSVDGTAFFLRGSGGEEPAELWGMRLVD"
     gene            complement(1693..2163)
                     /locus_tag="AC161X_06705"
     CDS             complement(1693..2163)
                     /locus_tag="AC161X_06705"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4989277.1"
                     /translation="MVQDRTDVDSFAEFVAVNEAGLRRALVASLGAELGREAAADALA
                     WGWEHWERLRGMAKPVAYLYVVGRNRGRKVASRRGPLFPPPPGENGVPWVEPDLPAAL
                     AALPERERTVVLMVHGYGMAYREVAETLGISRSTVQTHLERGLRKLRRRLGVAN"
BASE COUNT          425 a          826 c          660 g          410 t
ORIGIN      
        1 gcattgcgtc tcatcgtgtt cctcctcttg gcagagcacc tcacggcatc tccaccggta
       61 gtccttcccg tttccatgcc tccagccgcc agttgagcga taggacatcc cgatatccca
      121 tcgtccaggg ggacgaatca caccgaccta acggccaatg gggcgacctc ggcggtcagt
      181 cgaccagcct catcccccaa agttctgcgg gctcctcgcc accgctgcct ctgagaaaga
      241 aggcggtgcc atcgacggag ctcccccagg agaatcgctg ctcctcctcg tctccggcca
      301 ggatcccgac ctcactcgtg tcgaccctgc tccattgcct catatcggtg gagaagaaca
      361 tctccgacca cggagcatcg ccgtagacca cttcgccggc ccccaagttc acggtcctga
      421 ggaacgagcc ctgaagcggt atcccactgc tgaaccaggt gagaccgtca tcggagaaca
      481 tgacctccac ctcatggcca tcggcctgag catggacgac atctgccctc catcgatcgt
      541 cgaccaggct cacgaacccg ccagtgcccg gatatccgtc gcgttccatc gtctccatcc
      601 gaaccgggcc ctcccacgcg accccgtcgg acgaggtcca cgcctcgagg gtcggacatg
      661 gaccggctgg gcccgcgaag ctctccgtgc acgaggttcc gggaacagcg cctcgcacaa
      721 aggcaacgat tcgactgcca tcgaccacga ttgtcggctc tggggccatc tcggtggcga
      781 tgttctcgct gctccacgga gggcggacca cagtcgcggc gccgctcgga tcgatcagac
      841 tcagaagcac gccctcgtcg tagaagaacc agggcacgat cagattctcg ccccattcga
      901 tgggacccgg tgaatagttg ggatcccggc cgccctgcgc gcccacatct ccgacatcga
      961 cggttaccca ctccgagccg tcgagacgcc acaaactggg cgggttccaa tccaccgtcc
     1021 acaactcacc gttggtcgac cagactcccc cggggtcgcc gtcgacctga ggtagtgacg
     1081 gcatctgctc ccaactctcg ccgtcggggg tcttccaaag cccgtcatcg gtgcccaccc
     1141 agtattcgtc ctcgatcaac ccgaacgggg cgacgaaggc agggtcacca aggcctgcca
     1201 gtgagcccgg ctcccaggac ttgccgtcga gtgaggtcca gtagctgggg ccctccagag
     1261 agctcgagat gaccccgtag ccggttgcgg tctggaacac gtcgcccacc tcggggacag
     1321 tcgccgccga tccgttgacg taccaccact cgacagtccc gataggcgtc tccaggctcc
     1381 cctcctcgaa accggccctc ggaagcgacg aggtggatgt tgagccgggg accgacgtag
     1441 tcggcgggtt gtccaccacc ggcgcgtcac tacgcaggac atcgatcaag agagtgatgc
     1501 cagccatcaa ggccagtgct gctgccacga tcagcggtcg ccagcgatgc ccgtgtcccc
     1561 gatctatgtc ccgttggtcg ggcacctcct gaatcgtccc ggtgtgatgc atgatctcat
     1621 ccagagtcag gggcccgccg gagttctcca gaagctcgga gtagcgccgg agctcggtca
     1681 cgatatcgcg gctcaatttg ccacccccaa cctgcggcgc agcttgcgca accctctctc
     1741 caggtgggtc tggactgtgc tgcgggatat gccgagtgtc tccgccacct cccgatatgc
     1801 catgccatag ccgtgaacca tcagaacgac cgtgcgctcc cgctccggga gggcggcaag
     1861 ggcagccggg agatcgggct cgacccaggg cacgccattt tcgccaggtg gagggggaaa
     1921 caagggccct cgtctcgagg cgactttccg gcctcgattt cggcccacga cgtatagata
     1981 tgcgacgggc ttggccattc ctctcagtcg ttcccagtgc tcccaacccc atgcgagcgc
     2041 gtccgcagcc gcttccctcc ccaactccgc tcccagtgaa gccaccagag cgcgtcgcag
     2101 tcccgcttcg ttgacggcta caaactcggc gaaactgtcg acgtcagtcc ggtcttggac
     2161 cacaggagcc tttcgtgctc acctccattc aaggcttgga gagccacgat gcacgacagg
     2221 aatttggaac ctcgcccctg atcgtgccat cccgttcacg tcccatggag tgggtgcagc
     2281 cgggtccgtg gtccgcacga gctgcgtgct ctggcagccg a
//
LOCUS       DBNHXU010000266         3434 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4053783, whole genome shotgun sequence.
ACCESSION   DBNHXU010000266 DBNHXU010000000
VERSION     DBNHXU010000266.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3434)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3434
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4053783"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            81..155
                     /locus_tag="AC161X_06710"
     tRNA            81..155
                     /locus_tag="AC161X_06710"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:116..118,aa:Ile,seq:cat)
     gene            complement(597..1859)
                     /locus_tag="AC161X_06715"
     CDS             complement(597..1859)
                     /locus_tag="AC161X_06715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989278.1"
                     /translation="MTSMTSRSDLQPETTRRPTSRRAARLVWAVAGVGLVLIAWMGWL
                     AYQSGEFAKSPLAALAAIQLLLALVAFLLGGTVIVSRQPGNVIGWLLLIPGLTIAISE
                     LGTQWLLGLDPPPSSVNLGIWLLVWFTSWSWVLLIFPIFHLLLTFPNGKLLSPRWRWA
                     ASLEVVMISAMIGAVTFSESLQVATEDETLWTVPNPIGFFSDAAVETAFAGPVWSIGL
                     AAIALISASAVILRFRRGTREERQQLKWPLFAIAVFGLTYAFGGLTGEILPGSVADFL
                     FGFSIMAIPISIAIAVLRYRLYEIDRIVSRTVSYALVVGLLAGVFLGVISLLTSLIST
                     DSNVTVAASTLAVAALFNPLRRRTQGVVDRRFNRSRYDTQRVMDGFAASLRDQVDPEQ
                     VVTGWTNVVEETMQPASIGTWLRAPTTH"
     gene            complement(2167..2844)
                     /locus_tag="AC161X_06720"
     CDS             complement(2167..2844)
                     /locus_tag="AC161X_06720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-layer homology domain-containing protein"
                     /protein_id="HIR4989279.1"
                     /translation="MSHRRQIPVPQLIWTVVGALTLVLGIPLTVIASHQFNDVPDSNT
                     FHNSIAWLADNRITLGCNPPANTNFCPNDAVTRGQMAAFLRRQAQAYGNVGSQVTSAA
                     GNIGLTTAAFVVLSSVEVTPKAQAAVTLSAHAALEKSTSSNGTFMLRIARNTCNGTVV
                     GAARWRANTNADGGGSFEAITIPLTGSDVISSPVTYVLCGAKADVTLGNANVFERGLT
                     ATWSPTA"
     gene            complement(2917..>3434)
                     /locus_tag="AC161X_06725"
     CDS             complement(2917..>3434)
                     /locus_tag="AC161X_06725"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HIR4989280.1"
                     /translation="MATCRTLLRVSTGSGRPPGDVLAEVNDRIQPDIPSGMFVTCLLA
                     MIDPLSGSMAMANAGHNLPFQRSNTSTAEIMARGMPLGLMPDMFYEEIEGHLSPGDSL
                     VLTSDGLAEAHDPEGDMYGTDRLRHALSSANGDLVGAALESHTEFVGADWEQEDDITM
                     VTLARQLPSAG"
BASE COUNT          688 a         1050 c         1062 g          634 t
ORIGIN      
        1 gcaccatcga aatgatttgt acgattcgat cagcccgtaa ccggtcatca ttcctcgagc
       61 gaatgtcgag ccggtgtctc gggcccgtag ctcaatccgg tcagagcagc ggactcataa
      121 tccgttggtt gcaggttcaa gtcctgccgg gcccactcat cgactgtaag ggttttcgcc
      181 gtgtcgccag gccgcggctg gtcagagatg ttcgggaaga atgcccttcg caagatcgcc
      241 ggcgtggcgg cttcctgtcc cggtcagttc gccggactgc atcacccaac cccaggcttt
      301 cgcttgaagc cattcaggag tacgccgtag gcgcgtgagg tcctcttcgg gcaacggata
      361 gacggcgaga cgagttagaa gttccgccat acggtcggtg atctcaacac aggacattga
      421 gcgagtatgt cggccgcccg tgacagatcc tcatgggccg agcgaccttg ctcaccggtc
      481 tccagcttgg ggagcaaccc cttggcgctc tgctggtcag gctctcgacg acgagactcc
      541 gccccctgtc cagagacgga cacctacctg ctcgtctccc gacacagccc ttgatctcag
      601 tgggtggtcg gggctcgcaa ccaggtcccg atcgacgccg gctgcatcgt ctcctcgacc
      661 acgttggtcc agccggtcac cacctgttcg ggatccacct gatcgcgtag cgaggcggcg
      721 aacccgtcca tgactctctg ggtgtcgtat cgggagcggt tgaaacgccg atcgacaaca
      781 ccctgggtcc gtcgtcgcaa tggattaaac agggcggcaa ccgccaaagt cgatgcggcg
      841 acggtcacgt tggagtccgt cgagatgagc gacgtgagca ggctgatcac tcccaggaac
      901 acgccggcca gcagtccgac caccagggcg taggacacgg tgcgggagac gatgcgatcg
      961 atctcgtaga gccggtatcg gaggacggcg atggcgatcg agatcgggat cgccatgatc
     1021 gagaaaccga atagaaagtc ggcgacgctc ccaggcagga tctcgccggt caagccaccg
     1081 aaggcgtagg tcagtccgaa gacggcgatt gcgaaaagcg gccacttgag ttgttggcgc
     1141 tcttcgcgcg tacccctgcg gaacctgagg atcaccgcgg aggctgagat cagagcgatc
     1201 gcggccaatc cgatgctcca gaccggaccg gcgaacgcgg tctcgaccgc agcatcggaa
     1261 aagaacccga tcggattcgg aaccgtccac aaggtctcgt cttccgttgc gacctgcaga
     1321 ctttcgctga atgtgaccgc cccgatcatg gctgagatca tcacgacctc gaggctcgcc
     1381 gcccagcgcc agcgggggga gaggagcttt ccgttgggga aggtgagaag caggtggaag
     1441 atcgggaaaa tcagaagcac ccacgaccac gatgtgaacc agaccagcag ccatatcccg
     1501 aggtttaccg atgaaggcgg cgggtcgagc ccgagcaacc actgagtgcc aagctcactg
     1561 attgcgatgg tcaggccggg gatcagcaac agccaaccga tcacgtttcc cggctgccgg
     1621 ctgacaatca ccgtgccgcc gagcagaaac gccaccaagg cgagaagcaa ttggatcgcg
     1681 gccagagccg ctagcggact cttggcgaac tcaccggact gataagcgag ccatcccatc
     1741 caggcgatca acaccaggcc gacgcccgca accgcccaga cgagacgggc ggctcgacga
     1801 ctcgtcggcc tcctggtggt ctcgggttgc aagtcggatc gtgatgtcat cgacgtcatc
     1861 cagcccgatt tctagttgcc caccgtttcg aaatcgttac ggaattggcc gattgatgag
     1921 ttcgcgaaat cgtccgctgg ttcgcgaaaa atggaatgcg tcctgacttg catgttcggg
     1981 acggcatgtc taccttccca tttgtcggta cgggggcaac cccgaaccgg acccgaagag
     2041 accgagaggt cactccggga tcctctggga gaccaagctt tcgggtttgg agtccgagca
     2101 gggtcgtgaa ccccgagagg gtggaagctt cggccgaaac tccctcgggg tttttcgcgt
     2161 cctgggtcag gcagtcgggg accaggtcgc agtcagtcca cgctcgaaga cattggcgtt
     2221 cccgagagtc acatcagctt tcgccccgca aagcacatat gtcaccgggg aggagatcac
     2281 gtcgctaccg gtgagtggga tcgtgatcgc ttcgaatgat ccaccaccat ccgcgtttgt
     2341 gttcgcccgc catctcgcgg cgccgaccac cgtgccgttg caggtgttcc gtgcgattcg
     2401 caacatgaag gtcccgttgc tgctcgtcga cttttccagc gctgcatgtg cgctcagggt
     2461 taccgcggct tgcgcctttg gggttacctc aaccgacgac agcactacaa acgcggccgt
     2521 cgtgagaccg atgttcccag ctgcgctcgt gacctggctc ccaacgttgc catatgcctg
     2581 ggcctgacgt cgcaggaacg cagccatctg gccgcgagtc accgcatcgt ttggacagaa
     2641 attggtgttg gccggcggat tacagcccag cgtgatccga ttgtctgcga gccaggcaat
     2701 cgagttgtga aaggtgttgg agtcggggac gtcgttgaac tgatgagacg cgatgacggt
     2761 cagcgggatt ccgaggacca aggtcaacgc tcctacgacc gtccagatca actggggcac
     2821 agggatctga cgccgatgag acataggttc tcctttggtt ggagggaccc gggatggtca
     2881 tccggtcagg tcacgctacg ttcgggccgg tgactgtcaa ccggcggacg gcagttggcg
     2941 ggccagcgtc accattgtga tgtcgtcttc ctgttcccaa tccgctccca cgaactcggt
     3001 atgagactcg agcgccgctc cgacgagatc gccattggcg ctgctcagag catggcgcag
     3061 ccgatcggtc ccatacatgt ccccctccgg gtcgtgcgcc tcggccagac catcactggt
     3121 cagcaccagg ctgtcgccgg gagagaggtg accctcgatc tcctcataga acatgtcagg
     3181 catcaacccc agtggcatgc cgcgggccat gatctccgct gtggaggtat tgctccgctg
     3241 aaagggaagg ttgtgaccgg cgtttgccat cgccatcgag cccgacaacg gatcgatcat
     3301 cgccagaagg caagtgacaa acatgcccga cgggatgtca ggctggatgc ggtcgttgac
     3361 ctcggcgagc acatcaccgg gtggccggcc actgcctgtc gacacccgga gcagggtgcg
     3421 acaggtcgcc atca
//
LOCUS       DBNHXU010000267         2312 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4848919, whole genome shotgun sequence.
ACCESSION   DBNHXU010000267 DBNHXU010000000
VERSION     DBNHXU010000267.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2312)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2312
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4848919"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1484)
                     /locus_tag="AC161X_06730"
     CDS             complement(<1..1484)
                     /locus_tag="AC161X_06730"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4989281.1"
                     /translation="MTVSAPWERRVHFSIEVGQFIALVVAIVVDLINTEGQSSESWIA
                     VGVATVYVLGSAAVPEEWYHIRFGAEAITLAGAFLCAVAITLTGGPTSPYLLLSMGPP
                     VFATLYGGIRSGLMTGLLSAGLLALVTLSQDLPLVESAPAMALYLVFVLLVGVIRKLL
                     EDIHQQAAELAVEKESATQSLENLQEIHGVLVRLSQDVSAGRLNAVEVGAETLDTVLT
                     RLPGSAGKLAIEGENGLVVLAARGVPDPDGQVNQIPINTADSAVGMMELVTPGPLADD
                     DRAAVEAYLRPLGIAFANLQLLQDIAGSAVAEERLRLAREMHDEIGPSLASLGLSLDL
                     TAMQQADDPELVADLKVLRSNVTKLVEDVRAKVADLRSTPGPTLTARVLQATAEFNGN
                     PVIVVDIDERRPPRPALIADLTSIVTEAIRNAHNHSSALNILVSGRIHRDWGSCQVQD
                     DGIGFDPEYEPEGHFGLVGMRERADKLGATIDFRSTEGLGTTVT"
     gene            1509..2075
                     /gene="coaE"
                     /locus_tag="AC161X_06735"
     CDS             1509..2075
                     /gene="coaE"
                     /locus_tag="AC161X_06735"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR00152.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HIR4989282.1"
                     /translation="MTTENHRWLLSGGIGSGKSEVGRLFAASGIRVVDADRMGHRVLE
                     PAGKAFAAVADRWPEVLRDGVIDRGRLAGIVFADPGQLRELESLTHHHIFGTIHDELE
                     GFDGLAVVEVPVIDTGLGWPRMVVDASDEIRFERVLERGMASEDARARMASQPSRAQW
                     LASADLVIPNHGSREELAETVATVVSQL"
     gene            complement(2072..>2312)
                     /locus_tag="AC161X_06740"
     CDS             complement(2072..>2312)
                     /locus_tag="AC161X_06740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989283.1"
                     /translation="FGILLAYRLPASCEAPSDAINTVVMRAIMTCAVNADLPDERPGH
                     LIGLGTTEWVDPMRMQDIRSRLAQGDIEPDPVLLG"
BASE COUNT          432 a          697 c          745 g          438 t
ORIGIN      
        1 atagtcacag tcgtccctag cccttcggtt gatcggaaat caatagtcgc tcccaatttg
       61 tcggcacgtt cgcgcatccc cacaagccca aaatgccctt ctggctcata ttctggatcg
      121 aagccaattc cgtcgtcctg gacctgacaa ctgccccaat ccctgtggat gcggcccgaa
      181 accaggatgt tcagggcgga tgagtggttg tgggcgtttc tgatggcttc ggtcacgatc
      241 gacgtcagat ctgcgatcag ggcggggcgg ggcggccgtc tttcgtcgat atccaccacg
      301 atcaccggat tcccgttgaa ttcggctgtc gcctgcagca ctcttgccgt cagggtcggt
      361 cctggagtgc tcctcagatc ggccactttt gcccgaacat cctcgaccaa cttggtgaca
      421 ttggaccgaa gcaccttgag atctgcgacc aattcggggt catccgcctg ctgcattgcg
      481 gtgaggtcga gggagaggcc gagagaggcc agagaaggcc cgatttcatc gtgcatttcc
      541 cgggccaggc ggaggcgctc ctcagcgacc gcagagccgg caatgtcttg aagtagttga
      601 agattggcga aggcgatgcc gagcgggcgc agatatgcct ctacggcagc ccgatcgtca
      661 tctgccagtg gccccggggt gaccagttcc atcatcccca ccgcagaatc ggcagtgttg
      721 atcgggattt ggttcacctg gccgtccggg tcaggcacac cacgggctgc aagtaccacc
      781 aggccgtttt cgccctcgat cgccaacttc ccggccgacc cggggagccg ggtcaggaca
      841 gtgtccaggg tctcggcccc gacttctacc gcgttgagcc ggcctgcaga cacatcctgg
      901 gaaagtcgga ccaaaacccc atggatctcc tggagattct ccaggctctg ggtcgccgac
      961 tccttctcca cggccagctc ggctgcctgc tggtggatgt cttcgagaag tttgcggatg
     1021 accccgacca acaagacgaa aaccagataa agggccattg cgggggcgga ctccacgagc
     1081 ggcaggtcct gcgagagcgt gacgagggcg agcaggccgg ccgaaagaag acctgtcatc
     1141 aggccgcttc gaataccgcc atagagggtc gcgaacaccg gtggccccat ggaaagcagc
     1201 agatatgggc tggtggggcc gccggtgagg gttatcgcca ccgcacacag gaaggctccg
     1261 gcgagggtga tggcttctgc gccaaacctg atgtgatacc actcctcggg gaccgcggcc
     1321 gaccctaaga cgtagacggt ggccactccg acggcgatcc aggactcgct gctctgccct
     1381 tcggtgttga tcaggtcgac gacgatcgcc acaacaaggg cgatgaactg gccgacctcg
     1441 atgctgaagt ggacgcgtcg ctcccaagga gcgctgacgg tcatgaggta aggctaccgt
     1501 cgagcctggt gacgacagag aaccatcgct ggctgctctc cgggggcatc ggctctggca
     1561 agagcgaggt gggacggctg ttcgccgcct cgggcatcag agtcgtggat gcggatcgga
     1621 tgggccaccg ggttctggaa cccgccggta aggcttttgc cgcggtcgcc gaccgctggc
     1681 ccgaagtgct tcgggatggc gttatcgata gggggaggct ggctggcatt gttttcgctg
     1741 atccgggcca gctccgggaa ttggagtccc tgacccacca tcacatattc ggcacgatcc
     1801 atgatgagtt ggagggattt gacggtttgg ccgtggtgga ggtgcccgtc atcgacaccg
     1861 ggctcggttg gccgcggatg gtcgtcgatg cttccgatga gattcgcttc gagcgggtcc
     1921 tcgagcgggg catggccagc gaagacgctc gggcgaggat ggcgtcgcag ccatcgaggg
     1981 cccaatggct cgcctctgcg gacctggtga tccccaatca tgggagccgc gaggagctcg
     2041 cggaaacggt ggccactgtc gtgagccagc tctagccgag gaggaccgga tctggctcga
     2101 tgtctccttg tgccaaccga ctcctgatgt cctgcatcct catcggatcg acccattcgg
     2161 tagtcccaag cccgattaga tgcccgggcc tctcatccgg caggtcggcg ttgactgcac
     2221 aggtcatgat ggcgcgcatc acaacagtgt tgatggcgtc cgacggtgcc tcacacgacg
     2281 ccggaaggcg gtaagcaagc aggatcccga ac
//
LOCUS       DBNHXU010000268         3670 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3260409, whole genome shotgun sequence.
ACCESSION   DBNHXU010000268 DBNHXU010000000
VERSION     DBNHXU010000268.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3670)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3670)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3670
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3260409"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(147..443)
                     /locus_tag="AC161X_06745"
     CDS             complement(147..443)
                     /locus_tag="AC161X_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019434986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HIR4989284.1"
                     /translation="MMVWIDQDLCTGDGICEEITPDVFVGRDDGLWVVKEEAKHFGRT
                     VLFDGVEGEGHGPEGSRGLARIPDSLVADAIESAEECPGECIMIEPYREDAVLG"
     gene            complement(506..1021)
                     /locus_tag="AC161X_06750"
     CDS             complement(506..1021)
                     /locus_tag="AC161X_06750"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family deazaflavin-dependent
                     oxidoreductase"
                     /protein_id="HIR4989285.1"
                     /translation="MGLSTDLGYQYRAPNWAQRAVARVGTTRVVSAISRVTMPGLDRF
                     VLRLSGGKSAATTWLLGIPTLWLTTRGARSGELRRVPLFAIPIDDDLALLGTRFGYEA
                     TPGWVFNIEADPEVDVSYRGRSVHAAARAAFPEEESRIWERAGQIYPGYRFYADRAPH
                     RQIRVFVIESR"
     gene            complement(1042..1392)
                     /gene="ssrA"
                     /locus_tag="AC161X_06755"
     tmRNA           complement(1042..1392)
                     /gene="ssrA"
                     /locus_tag="AC161X_06755"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1498..1980)
                     /gene="smpB"
                     /locus_tag="AC161X_06760"
     CDS             complement(1498..1980)
                     /gene="smpB"
                     /locus_tag="AC161X_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011697642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HIR4989286.1"
                     /translation="MGRSEPSGRFKIIASNRRARHNYAVVETFEAGAVLFGSEVKSLR
                     EGRMDLKDSYGLVRGGEMFLVGSYIGPYEFAREGGHEPERDRKLLLHRREIDRIAGQV
                     AEKGLTLVPLQVYFKEGKVKVEIGLAKGKTSYDKRQTLRDRDHDREMERASSQARRKS
                     "
     gene            complement(1981..2925)
                     /locus_tag="AC161X_06765"
     CDS             complement(1981..2925)
                     /locus_tag="AC161X_06765"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsX"
                     /protein_id="HIR4989287.1"
                     /translation="MSRLFFLVREALINLRRNLLVVAGAVLAVFISLTLAFAALVANE
                     VLRTWIAGWQEGVHVIAFLNDEGANGVPAGAHQELLDEVLTWEEVKQPDGAFYVSKAQ
                     AWEEYQKLFAGQDELLEIDPTVLPASIRIELVDIALHESVAFKLREQQQVVREVRTAA
                     DEIEQLQSLRDVVRVLGIGAAVVLGLAAVVLIANTIRLAIYARRDEVEIMRLVGASNW
                     YIRIPFLLEGMIEGLLGAAAAVFVVWIGATRLSDAFSGFGLFRFEVDSDFFLRWGLMF
                     LLFGALAGVVGSMLGLSRHLRESEGVGSEVPTGTLERV"
     gene            complement(2922..>3670)
                     /gene="ftsE"
                     /locus_tag="AC161X_06770"
     CDS             complement(2922..>3670)
                     /gene="ftsE"
                     /locus_tag="AC161X_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013251033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HIR4989288.1"
                     /translation="LPSSVLRLEADACETAPDPMIRLENVTKVYEGGTIAAHDVSFEM
                     QKGEFTFLVGPSGSGKSTLIRLMLRDEPVTRGKIWVAGKDITTLPKWKIPFLRRSVGT
                     VFQDYKLLPNKTVGENVAFALEVLGRPRSVIGPQVEQVLDLVGLSDKVARYPKQLSGG
                     EQQRVSVARAFVNRPPIMLCDEPTGNLDPKTSVEIMKLLDRINRTGTTIVMATHDHAI
                     VDAMQRRVVALDQGRVMRDEEAGKYETVPE"
BASE COUNT          711 a         1196 c         1074 g          689 t
ORIGIN      
        1 tcgatcatcg tcatgatccg ggtgacggtc ccgggcttct taccgacgcg gcgcgaaatc
       61 tcaggcggct cctggccggc ttcgctgagg cggacgatca ctcgctcgat tggtcgtagg
      121 tcctggtgtt ccactacctg ctccttctat ccgagaaccg catcctcccg atagggctcg
      181 atcatgatgc attcccccgg gcattcctcc gcagactcga tggcgtcggc gacaagtgag
      241 tccgggatcc gggcgagacc tcgggagccc tccgggccgt gaccttcgcc ctccaccccg
      301 tcgaaaagga cggtccggcc gaaatgcttg gcctcctcct tgacgaccca caagccgtca
      361 tctctgccca caaacacgtc gggcgtgatc tcctcacata tcccgtcacc ggtgcacagg
      421 tcttggtcga tccaaaccat caactgtgtc ttgtcggcca attcgtcacc tcgtgctcaa
      481 gccactcgct gcgaccctat cgcactcatc gagattcgat cacaaagacc cggatctggc
      541 ggtgcggggc acggtccgcg tagaaccgat agccgggata gatctggccg gcgcgttccc
      601 aaatccggct ctcctcctcc ggaaatgccg ctcttgcagc tgcatggacc gatctccccc
      661 gatacgagac atcgacctcg ggatcagcct caatattgaa gacccagccc ggagtcgcct
      721 cgtacccgaa tcgggtcccg agcaaagcca ggtcatcgtc gatgggaatg gcaaacaagg
      781 gcactcggcg caactcccca ctgcgggcac cacgggtcgt cagccaaagc gtcggaatcc
      841 ccagcagcca ggtggtggca gcggactttc caccgctgag tctcaggaca aacctgtcca
      901 aacccggcat cgtcacgcgg gagatggccg atacgacccg tgtcgtcccg actcgggcga
      961 ccgcgcgttg cgcccaattc ggggcccgat attggtaacc gagatccgtg gacagtccca
     1021 cgccgcgatc gtaggtagag atggtggagg tgcggggagt cgaaccccgg tccgtcgagc
     1081 cctcggcgtt ggcatctccg agcgcagtcg tcagcgagct ttcggcggcg ggaactctga
     1141 cgacgagttt tcccgtgcct atccggagtt gtcttagccc cacggtcccg gaatcccgtg
     1201 gagagcgtcc cgcagtgcga cgcccgatcc ctgccgtgcg ggagccggcc gggcggacgg
     1261 gctacttaat taggcagcca gtgcgaagtt atcttcggca cttattggtt tttccggttt
     1321 gttgacgagg ccccggagac cccggctcgc ttccaacgct tcaacactcg acgtcgaaac
     1381 cgtttcaccc ccatggggag ggacaggtta cccgtcggga acggttgtcc aatcccgatt
     1441 ggttccaagt ctcaagtctt aagccggagt tctggcctga gacctgcgac ttgagactta
     1501 tgacttccgc ctcgcttgcg aggaagctcg ctccatctcg cggtcgtggt cccggtcgcg
     1561 aagggtctgt cgcttgtcat aggaggtctt gcccttggcg aggccgatct cgacctttac
     1621 cttcccctcc ttgaagtaga cctggagcgg aacgagcgtc aggcccttct cggcaacctg
     1681 gccggcaatc cgatcgattt ccctgcggtg cagaagcagc ttgcgatcgc gctccggctc
     1741 gtggccacct tctcgagcga actcgtacgg gccgatgtag gagccgacga gaaacatctc
     1801 cccgccgcgg accaatccgt atgaatcctt gagatccatg cgcccctcac gcaacgactt
     1861 cacctccgac ccgaacagga ccgcgcctgc ctcgaaagtc tccaccaccg cgtagttgtg
     1921 acgcgcccga cgattcgagg cgatgatctt gaaacgcccg gacggctccg agcggcccat
     1981 tcagacccgc tccaacgtcc ccgtgggcac ttcggagcca accccttccg actccctcag
     2041 gtgtcggctc aggcccagca tcgatccgac cacaccggcc agggctccaa acagcaggaa
     2101 catgagaccc caacgcagga agaagtcgct gtccacttca aaccggaaca gcccgaagcc
     2161 ggagaacgcg tcggagaggc gggtggcgcc aatccacacc acgaagaccg cggccgcagc
     2221 tccaagcagg ccttcgatca ttccctccaa caggaacggg atcctgatgt accagttcga
     2281 ggcgccaacc agcctcatga tctcgacctc atcgcggcgg gcgtaaatgg cgaggcggat
     2341 ggtgttggcg atgagcacca cggcggcaag cccgagcacg acagcggcac cgatcccgag
     2401 aactctgacc acatctcgca ggctttgcag ctgttcgatc tcgtcggccg cggtacggac
     2461 ctcgcgaacc acctgctgct gctctcgaag tttgaacgcg acggactcgt ggagtgcaat
     2521 gtcgacaagc tcgatccgaa tcgaagccgg aaggaccgtg gggtcgatct ccagaagctc
     2581 gtcttgacca gcgaagagct tctggtattc ctcccaggcc tgcgccttgc tcacatagaa
     2641 ggctccatct ggctgcttca cctcttccca ggtcaagacc tcgtctaaca actcttggtg
     2701 ggccccagcg gggacaccgt ttgcgccctc atcgttcagg aaggcgatga catggacacc
     2761 ctcttgccag ccggcgatcc aggtgcgaag gacttcgttg gcgaccagag ctgcaaacgc
     2821 cagggtcagc gagatgaata cggcaaggac cgcgccggcg actacgagga ggttgcgtcg
     2881 gagattgatc agcgcttccc ggaccaggaa gaagagacga ctcactccgg caccgtctca
     2941 tacttgccgg cttcctcgtc cctcatcacg cggccttggt cgagggcgac gacacgtcgc
     3001 tgcatggcgt cgacgatggc gtggtcgtgt gtggccatga cgatcgtggt cccggttctg
     3061 ttgatccggt cgaggagctt catgatctcg actgaggtct tcgggtcaag gtttcctgtc
     3121 ggctcatcgc acagcattat tggtgggcgg ttgacaaaag cccgggcaac cgagactcgt
     3181 tgctgctcgc caccggacaa ttgcttgggg taccgggcaa ctttgtcgga gagcccgacc
     3241 aggtcgagaa cctgctcgac ttgcggcccg atcacggaac ggggccgtcc caagacctcc
     3301 aaggcgaagg caacgttttc gccgacggtc ttgttgggaa gaagcttgta gtcctggaag
     3361 acagtcccca ccgagcgccg cagaaacggg atcttccact tggggagggt ggtgatgtcc
     3421 ttcccagcga cccaaatctt gccccgagtg actggctcat cccgaagcat gagccggatc
     3481 agggtcgact tgccggatcc ggaagggccc acgaggaaag tgaactcacc cttttgcatc
     3541 tcgaatgaga cgtcgtgggc agcaatggtg ccgccctcat agaccttggt gacgttttcg
     3601 agtcggatca tgggatcagg agcagtctcg caggcatctg cctccagccg cagaaccgag
     3661 gacggtaacc
//
LOCUS       DBNHXU010000269         2387 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3260641, whole genome shotgun sequence.
ACCESSION   DBNHXU010000269 DBNHXU010000000
VERSION     DBNHXU010000269.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3260641"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            544..1476
                     /locus_tag="AC161X_06775"
     CDS             544..1476
                     /locus_tag="AC161X_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016750176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989289.1"
                     /translation="MATFLTRALHLTPNKPPARCPILPADDIWNTRVDNLPVHSRSAA
                     YVASIGNSATFHADFGSGVWPPGSNSPIGIPYVEVPQGTPALTIHYTSYGNESDPGPF
                     PIPTNTPVEGGPDSDGDRHVIVVDRFRCELFELFSAYPGPGNSWTAASGARYELDSSD
                     LRQDGWTSADAAGLPIFPGLVRYDEVASGYIGHAIRFTAPDTQAAHVWPARHDASDLT
                     GLNFPPMGQRFRLKASFDISGFSPQVRVILRAFKEYGIVLADNGSSWYVSGAPDSRWN
                     NDVLHELDQIHGSAFEAVNVSSLMVDPDSGQAQP"
     gene            1521..2108
                     /locus_tag="AC161X_06780"
     CDS             1521..2108
                     /locus_tag="AC161X_06780"
                     /inference="COORDINATES: protein motif:HMM:NF022441.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIN domain-containing protein"
                     /protein_id="HIR4989290.1"
                     /translation="MRRWWVAAAGLSMTLAACGPSGSGNVVSDQRDVGVFDSIEVSGG
                     IALRLNVDPSATVSVTVNYDDNIIDLIVTEVDGTTLQIESKGTYNTIGGEDRYVEVTV
                     PTLLGLTASGGADVIGQGALDVLDVNASGGANVDLALLPVGDVTLEASGGANIVVNVT
                     DAITGTASGGADVSVQGDPPLQNIDVSGGADLSNG"
     gene            2112..>2387
                     /locus_tag="AC161X_06785"
     CDS             2112..>2387
                     /locus_tag="AC161X_06785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989291.1"
                     /translation="MTRQPGFSNVPSTVSAEWADYFGNLPDPSTLPLLPAPGDFATWK
                     ERNRQAEAERLAAGFGDPGPGVEVVEREIGGVAVLEALPQDRIEDGRA"
BASE COUNT          403 a          758 c          754 g          472 t
ORIGIN      
        1 gcgctcgtgt tggcggtgtt gttggcggtt ctgcctccgg gtgggtcgtt tgtggatgat
       61 gatgggaatg ttcatgaggg tgggatcgag gcgatcgcgg cggcggggat caccaagggt
      121 tgtaaccctc cggccaatga ccggtattgc ccttcgcgga gtgtgacccg gggggagatg
      181 gcggtgttct tgtcccgggc gttgggtctg cccgcgacct ctcagaactt cttctccgac
      241 gacaacggac acattttcga gggtgggatc aaccggttga gggcggcggg gatcactcag
      301 ggttgtaacc cgcctgcgaa cagccggttc tgtcccgacc gggagatgac ccggggggag
      361 atggcggcga tgctggtccg ggcgtttcac tatccggctt cgtctcagaa cttcttcacc
      421 gacgacaacg gacacatttt cgagggtgcg atcaaccggt tggcagcggc ggggatcact
      481 gctgggtgta acccgcctgc gaacagccgg ttctgtcccg accagaagat caaacgggat
      541 cagatggcca ctttcctcac ccgagccctc cacctcaccc ccaacaaacc accagcacgt
      601 tgccccatcc ttcccgccga cgacatctgg aacacccgtg tcgacaacct cccggttcac
      661 tcccgctccg ccgcctacgt ggcctcaatt ggcaactcgg ccaccttcca cgccgacttc
      721 ggttcgggag tctggccacc cggctcgaac agccctatcg gtatcccgta tgtcgaggtg
      781 ccgcagggaa ctccggcgct gacgatccac tacaccagtt atggcaacga gtccgaccct
      841 ggcccgttcc cgatccccac caacacaccg gtagaaggcg gccccgactc cgatggcgac
      901 cggcacgtga tcgtggtcga tcgcttccgg tgcgagctct tcgagctctt ctccgcctat
      961 cccgggccgg gcaactcatg gaccgccgcc tcaggggcgc gctatgagct cgattcttcc
     1021 gacctgcgtc aggacggctg gacctccgct gatgccgccg gtctgcccat cttcccgggg
     1081 ctggtccgct acgacgaggt tgcctccgga tacatcggcc acgccattcg attcacggcg
     1141 cccgacaccc aggctgctca cgtctggccg gcgcggcatg atgcgtcgga cctcaccggt
     1201 ctcaattttc ccccgatggg acagcggttc cggctcaaag ccagtttcga catctccggt
     1261 ttctctcccc aggtgcgagt catcctcaga gccttcaagg aatacggaat tgtcctcgcc
     1321 gacaacggat cctcttggta cgtctcaggg gcgcccgatt cgcgatggaa caacgacgtt
     1381 ctccacgagc tggatcagat acacggttcg gctttcgagg cggtcaacgt gtcgtcactc
     1441 atggtcgatc cggactccgg acaagctcag ccctgatcct tttcgatagg tttgcagcct
     1501 gtcgacagga gatgatgctg atgaggcgtt ggtgggttgc ggccgctggg ctcagcatga
     1561 cgctggcagc ctgcggtccc tctggatcgg ggaatgtcgt gagtgaccag cgcgacgtcg
     1621 gagtcttcga cagcatcgag gtgtccggtg ggatcgccct ccggctcaat gtcgacccgt
     1681 cggcgactgt ttcggtcact gtcaactatg acgacaacat catcgatctc atcgtcaccg
     1741 aggttgatgg caccacgctc cagatcgaga gcaaaggcac gtacaacacc atcggtgggg
     1801 aagatcgcta cgtcgaagtg acggtgccca cgcttctcgg gctcaccgcc tcgggtggag
     1861 ccgacgtgat cggacagggg gcgctcgatg tcctggatgt gaatgcgagc ggtggtgcaa
     1921 acgtcgacct tgccctgctc ccggttggag atgtgactct cgaggcatcg ggcggtgcga
     1981 atattgtcgt gaacgtgacc gacgcgatca cagggacggc ttccgggggt gccgatgtct
     2041 cggttcaggg cgatccgccg ctgcagaaca tcgatgtcag cggtggcgcc gatctgagca
     2101 atggctgacg attgacccgg cagcctggct ttagcaacgt tccgtccacc gtctccgcgg
     2161 agtgggccga ctacttcggg aatctgcccg acccgtcgac ccttccgctg ttgccggcac
     2221 cgggggactt cgcgacctgg aaggagcgca atcgtcaggc cgaggccgag cgtctggcag
     2281 ccggcttcgg ggaccccggc cccggtgtgg aggtcgtcga gcgcgagatc ggtggggtag
     2341 cggtcctcga ggctctgccc caggatcgga tcgaggatgg ccgcgcc
//
LOCUS       DBNHXU010000270         2737 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2069843, whole genome shotgun sequence.
ACCESSION   DBNHXU010000270 DBNHXU010000000
VERSION     DBNHXU010000270.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2737)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2737)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2737
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2069843"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..782
                     /locus_tag="AC161X_06790"
     CDS             <1..782
                     /locus_tag="AC161X_06790"
                     /inference="COORDINATES: protein motif:HMM:NF019229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="CRTAC1 family protein"
                     /protein_id="HIR4989292.1"
                     /translation="FEDVTPADYAAVPVREEDDAAGVAIGDLDKDGKPDLVVGQHYRA
                     TVEFDSEIPVRVFLNTDDGFQDVTEAAGLTPLPTLAPHVEIADIDNDSWLDIVTSASA
                     EDGTAPAVYRNLGGEDLSFEVSTGLGSDQYWVGAPVVDIDRDGRLDVFAVEWEPSLPS
                     LMFHNNGDSGHWLEVSIDEPGRGIGTLVTVSTEDGTQIGREEISAGGGYASGHLPVAH
                     FGLGSETQVSVTIQHPDGSITELPGIVADQHLRWPQGCGTG"
     gene            838..2115
                     /locus_tag="AC161X_06795"
     CDS             838..2115
                     /locus_tag="AC161X_06795"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR4989293.1"
                     /translation="MTDLVTRHKDVIAPVIAFDTDIHAVKAEGIWVTDIEGRRWADFA
                     CGTAVVNLGHNHPAVVEAAHRQIDSLMHSGCIVRYDSIVDAAERLRDITPPGIQKFAF
                     ANSGAEAVEAAVKLAKYSTGRQGVVAFRGAFHGRTMGSISYTTSNAKYRDGYHPVLGS
                     VFVAPFPHPYRWGMTESEAVDHALNELRMTFKHVVTPKNIACFLVEPVQGEGGYYPAP
                     PAFLDALRELANENGIMVIYDEVQTGFGRTAEWFAADHFDAKPDIMAMGKGIANGLPL
                     SAYGSSVEVMDNWPVGAHGTTFGGNPVSTAAAVAVMDTMDGLLPHARELSKRVFERFH
                     EMAESHPTIGDVRGLGLMTGVELVTDRESRTPDKPAFQHVEKHAYDRDLIVINCGPDG
                     NVIRFIPPLVATTEEVDWAMDLVDEGLSSWESR"
     gene            complement(2119..>2737)
                     /locus_tag="AC161X_06800"
     CDS             complement(2119..>2737)
                     /locus_tag="AC161X_06800"
                     /inference="COORDINATES: protein motif:HMM:NF013868.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HIR4989294.1"
                     /translation="ALVELVDIDSLKELYRTYSDELGKIYGVAARPDFIAALAAMLGV
                     LIFDTLPGLFIGIIVSILLMVYRASRPHVADLGVVPGTDIYIDRTRRPDAVAPEGIAV
                     IRVESGLFFANADYVRARVREAAATPGTKAVIIDAETIPAIDVTAVRMLIEVTDELRA
                     DGVDLILAHDIGQVRDLLPADERGTGPALYPSIDQAIVAVRERSG"
BASE COUNT          510 a          883 c          874 g          470 t
ORIGIN      
        1 ccttcgaaga cgtgacccct gccgactacg ccgcggtacc agtcagggag gaggacgacg
       61 cggcaggggt tgccatcggt gatctcgaca aggatgggaa gcccgaccta gtggtcggcc
      121 agcattaccg ggcgacggtg gagttcgact ccgagatccc ggtgcgggtt ttcctgaaca
      181 cggacgacgg atttcaggac gtgaccgagg cggccgggct gacgccgctt cccacccttg
      241 cccctcacgt cgagatcgct gacatcgaca acgactcctg gctcgacatc gtcacttcgg
      301 cctcggcgga ggacgggacc gcacccgcgg tgtaccgcaa cctcggtgga gaggatctgt
      361 ccttcgaggt ttccaccgga ttgggctctg accagtattg ggtcggcgct ccggtggtcg
      421 acatcgaccg ggacggacgt ctcgatgtgt tcgccgtgga atgggagcca tcgctcccct
      481 cactgatgtt ccacaacaac ggggacagtg gtcattggct cgaagtctcg atcgatgagc
      541 ccgggcgggg catcggcacc ctggtcacgg tgtccaccga agacggcacc caaatcggcc
      601 gtgaggagat cagcgccggg ggcggatatg cctccggtca cctcccggtc gcccatttcg
      661 gcctcggatc cgaaacccag gtttcggtca ccatccagca cccggacggg tccatcaccg
      721 aactgcccgg aatcgtcgct gaccagcacc ttcgctggcc ccaggggtgc ggcacgggct
      781 agagagccca ccgtccgggg ttcccgatat caggcccgtt gggtagcatc gcgccccatg
      841 accgacctcg tcacccgcca caaggacgtg atcgcgcccg tgatcgcctt cgacaccgat
      901 attcatgccg tgaaggcgga agggatttgg gtgaccgaca tcgaaggccg gcgctgggct
      961 gacttcgctt gcggcaccgc ggtggtcaac ctcggtcaca accatcccgc agtcgtcgaa
     1021 gcggcgcatc gccagatcga cagtctcatg cactccgggt gcatcgtccg ttacgactcc
     1081 atcgtggacg ccgccgagag gcttcgggac ataaccccgc ccggcatcca gaagttcgcc
     1141 ttcgctaatt ccggcgccga ggcggtcgag gcggccgtga agctggccaa gtactcgacc
     1201 ggtcgccagg gcgtcgtcgc tttccgcggg gcgtttcacg gacggaccat ggggtcgatc
     1261 agctacacga cctccaacgc caagtaccgg gacgggtatc acccggtact ggggtcggtt
     1321 ttcgtggccc ccttccccca cccttatcgc tgggggatga ccgaatccga ggccgtggat
     1381 cacgccttga acgagctgcg gatgaccttc aagcatgtgg tcaccccgaa gaacatcgcc
     1441 tgcttcctgg tggagccggt gcagggtgag ggcggctact acccggcccc gcctgcattc
     1501 ctcgacgccc tccgggagct ggccaacgag aacgggatca tggtgatcta cgacgaggtg
     1561 cagactggat tcggccgcac cgcagagtgg tttgccgctg accacttcga cgccaagccc
     1621 gacatcatgg ccatgggcaa ggggatcgcc aacgggttgc ccctctccgc ctatgggtca
     1681 tcggtcgagg tcatggacaa ctggccggtg ggggcccatg gcaccacctt tggcgggaac
     1741 ccggtgtcga ctgcggcggc ggtcgcagtg atggacacca tggatggcct gctccctcat
     1801 gcccgggagc tgtcgaagcg agtcttcgaa cgcttccacg aaatggcgga gagtcatccg
     1861 acgattggcg acgtgcgcgg actgggattg atgaccggtg tcgagctggt gaccgatcgg
     1921 gagagcagga cccctgacaa gcccgccttc caacacgtcg agaagcacgc ctacgaccgc
     1981 gatctgatcg tcatcaactg cggccctgac gggaacgtta tccggttcat cccacccctg
     2041 gttgccacca ccgaagaagt ggactgggcg atggacctgg tggacgaggg cctcagcagt
     2101 tgggagagcc gctagcggct aacccgacct ctctctgacc gcaacgatcg cctggtcgat
     2161 gctcggatag agggcaggcc cggtccctcg ctcgtcagcg gggagcaagt cccgcacctg
     2221 tccgatgtcg tgggcgagga tcaaatcgac accgtcggcc cgcagctcgt cggtgacctc
     2281 gatcagcatc cgaaccgcag tgacatcgat agcagggatg gtctcggcgt caatgatcac
     2341 tgctttcgtg cctggagtgg cggccgcctc acgcacccga gcgcgcacat agtcggcatt
     2401 ggcgaagaag aggccgctct caacgcgaat caccgcaatt ccctccggag cgaccgcgtc
     2461 gggccgacgg gtcctatcga tgtagatatc ggtcccgggg accactccca agtcggcaac
     2521 atgaggcctc gatgcccgat agaccatgag cagaatcgaa acgatgatcc cgatgaagag
     2581 cccgggcagc gtgtcaaaga tgaggacccc gagcatcgct gcgagagcgg cgatgaagtc
     2641 cggccgggcc gcaaccccat agatcttgcc cagctcgtcg ctgtaggtgc gatagagctc
     2701 cttgagagag tcgatgtcga ccagttcgac gagtgcc
//
LOCUS       DBNHXU010000271         4944 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1872418, whole genome shotgun sequence.
ACCESSION   DBNHXU010000271 DBNHXU010000000
VERSION     DBNHXU010000271.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4944)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4944)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4944
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1872418"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="AC161X_06805"
     CDS             complement(<1..297)
                     /locus_tag="AC161X_06805"
                     /inference="COORDINATES: protein motif:HMM:NF046506.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6941 family protein"
                     /protein_id="HIR4989295.1"
                     /translation="MDLAPDSLGPTVDVALLADAVQAVRGKLFVLGGGWDTLWVRSFP
                     ARHPSLAIGLRLRVPSSWGEDSLKVSVELQDADGAAMLPQPLAHVVTLPGHSQPS"
     gene            366..1691
                     /locus_tag="AC161X_06810"
     CDS             366..1691
                     /locus_tag="AC161X_06810"
                     /EC_number="5.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015424187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004512 - inositol-3-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006021 - inositol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
                     /protein_id="HIR4989296.1"
                     /translation="MTDKTQIAPATGKLGVLTPGMGAVSTTFIAGVLAARKGDATPFG
                     SLTQMSHIRLGKRTEQRNPLIKDFVPLAGLDDLEFGGWDPYSDDAYTSAMKAGVLDPR
                     HIEGIGDELRSVKPYSAVFSENWVKNLDGVDQIKEGANYMDLAERLMEDIDRFRSEKM
                     VERLVMVWCGSTEAYRDLSEAHLDLDSFVKGLKESDPTISPSQIYAYAALKMGVPFAN
                     GAPNLTLDVPAMVQLATEEGLPIAGKDFKTGQTLMKTIVAPGLKARMLGIRGWFSTNI
                     LGNRDGEVLDDPESFRSKEVSKLGVLEQILQPEVYPELYGDLYHKVRINYYPPKGDDK
                     EGWDSIDIFGWLGYPMQIKIDFLCKDSILAAPIVLDLALFMDLAARADRSGVQEWLSF
                     YFKSPQHGQGLYPENDLFIQHTKLKNNLRHMMGEDLITHLGTEYYDDEM"
     gene            1688..2095
                     /gene="arfB"
                     /locus_tag="AC161X_06815"
     CDS             1688..2095
                     /gene="arfB"
                     /locus_tag="AC161X_06815"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020077092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HIR4989297.1"
                     /translation="MTGEGITVGGWLIPESELEERFDTSGGPGGQHANRNETAVTLRF
                     SIADSSLPPDLQERLIRRLGSWVEVNAADTRSQWRNREVARERLIAKLEDALVVPKPR
                     KKTKPSKAAKERRLETKKARSDVKKKRRPPEVD"
     gene            complement(2109..2615)
                     /locus_tag="AC161X_06820"
     CDS             complement(2109..2615)
                     /locus_tag="AC161X_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE/DsrF/DrsH-like family protein"
                     /protein_id="HIR4989298.1"
                     /translation="MTMTLEAPVPVFDDESTDRKLAIICSKGSLDMAYPGLVLANAAL
                     GEGIETHVFFTFWGFDMINKETMAHLKFSPVGNPATHMSPMLMGLPGMTSFATHMMKK
                     QMEGIDVPGVPEFLEMITDAGGHLWACRMSADMMHIKEEDLYERVEGIISAADFIEIS
                     EGAQIIFV"
     gene            complement(2626..2940)
                     /locus_tag="AC161X_06825"
     CDS             complement(2626..2940)
                     /locus_tag="AC161X_06825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012184598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HIR4989299.1"
                     /translation="MTVATMRGRELHVDAEGFLTESSEWDEGLAETLASNISLELTER
                     HWAAIRFARSDFAASGEAPTLRRMATAGGVPTKEMFELFPKKPAKKLAYVAGLPKPVG
                     CV"
     gene            complement(2937..4196)
                     /gene="sqr"
                     /locus_tag="AC161X_06830"
     CDS             complement(2937..4196)
                     /gene="sqr"
                     /locus_tag="AC161X_06830"
                     /EC_number="1.8.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020524776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III sulfide quinone reductase,
                     selenoprotein subtype"
                     /protein_id="HIR4989300.1"
                     /translation="MKRLVVLGAGTAGTMIVNKLRPKLPKEEWEITVVEPAVSHLYQP
                     GLLFVPFGRYESDDLRKPTRALLPDGVELVTEPVKRVDVESNRVHLAGGRNLDYDQLV
                     IATGTHPRPEMTPGMDGPELGDTVHEFYTMAGAEALRWKLALWDGGRFVVHITELPIK
                     CPVAPLEFAFLADDYFRQIGIRDRVEMTFATPLSGAFTRPIASAQLGDMLAARDIHVE
                     PDFYVERIDDHALVSYDERVIPFDLLVTVPVNMGADFVAGSGLGDELNHVRVDPHTFL
                     ADGCDNVFAIGDAAALPISKAGSVAHFAVDVFVENFIDHIAGRPMERSFDGHANCFVE
                     SGSGKALLIDFNYETEPLPGTYPVPGVGPLSLLKETRINHLGKLAFRPIYWHLLLPGR
                     RLPVPNAMSMAGKEIPTAQPPREDVTI"
     gene            4400..>4944
                     /locus_tag="AC161X_06835"
     CDS             4400..>4944
                     /locus_tag="AC161X_06835"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR4989301.1"
                     /translation="MRTGNRVALVAALALAVASCGSDGSTTTIDRTTSTVPETTISLT
                     APATTTTISSTSTSAATAGRTVYFLLDEVGGSSGVGPFLVPVYREGPESDDAASDAVE
                     LLLAGPTAGEMAGTPAMQTAIPEGTSLLGLSVDDGVATVDLSAEFDDGGGSAGMFARL
                     AQVVFTVTRATGVDEVTFELA"
BASE COUNT         1008 a         1515 c         1513 g          908 t
ORIGIN      
        1 ggatggctgg ctgtgaccgg gaagagtgac tacgtgggcg agtggttggg gcagcatcgc
       61 ggcgccatcc gcgtcttgaa gctcaacgga caccttcaac gagtcctcgc cccaggaaga
      121 tgggacgcgg agccgaagac cgatcgccag gctgggatga cgggctggaa acgagcggac
      181 ccaaagggtg tcccagccgc cacccagcac gaatagcttg cctcggactg cctggaccgc
      241 gtccgcgagg agcgcaacgt ccacagtcgg gcctagcgaa tcgggggcta gatccacgag
      301 ctgagactag acccgggttc cgctgaccca atcagctctt ctatcgttcc gggtcaggag
      361 gatcgatgac agacaaaact cagatcgccc cggccaccgg caagcttggc gtcctcaccc
      421 ccgggatggg cgcggtctcc accacattca tcgcaggcgt gctggcggcg cgcaagggcg
      481 atgcgacacc attcggatcg ctgacccaga tgtcccacat acggcttggc aagcgaaccg
      541 agcaacgcaa cccgctgatc aaggatttcg tcccactggc cggcctcgat gacctcgaat
      601 tcgggggatg ggatccctac tccgacgacg cctacacctc agccatgaag gcaggcgtcc
      661 tcgacccccg tcacatcgag ggaattggag atgagctgcg ctcagtcaag ccctactcgg
      721 ccgtgttctc ggagaactgg gtcaagaacc tcgacggagt ggaccagatc aaggaagggg
      781 ccaactacat ggacctcgcc gagaggctca tggaggacat cgaccggttc cgctccgaga
      841 agatggtgga acgcctggtg atggtttggt gcggctcgac cgaggcctac cgcgacctgt
      901 ccgaggccca tctcgacctc gactccttcg tcaaaggcct caaagagagc gaccccacca
      961 tttccccatc gcagatttac gcctatgcgg cgttgaagat gggcgttcca ttcgccaacg
     1021 gcgctcccaa cctcaccttg gacgttccgg cgatggtcca gctcgcaaca gaagaaggtc
     1081 tcccgatcgc gggcaaggac ttcaaaaccg gacagaccct catgaagacc atcgtggctc
     1141 ctgggctcaa ggctcggatg ttgggaatca ggggatggtt ctcgaccaac atcctcggga
     1201 atcgcgacgg ggaggtcctc gacgacccgg agagcttccg atccaaggag gtttccaagc
     1261 tcggcgtcct cgagcagatc ctgcagcccg aggtctatcc cgagctctac ggcgacctct
     1321 atcacaaggt gcggatcaac tactacccgc ccaagggcga cgacaaggag ggttgggaca
     1381 gcatcgacat cttcggatgg ctcggctatc cgatgcagat caagatcgac ttcttgtgca
     1441 aggactcgat tctggccgcc ccgatcgtcc tcgatctagc cctcttcatg gatcttgcgg
     1501 cacgagccga ccgcagtggt gtccaggagt ggctgtcgtt ctacttcaag agccctcagc
     1561 atggccaggg gctatatccc gagaacgatc tattcatcca gcacaccaag ttgaagaaca
     1621 acctgcgcca catgatgggt gaagatctga tcacccacct cggcaccgag tactacgacg
     1681 acgagatgtg accggcgagg ggatcaccgt cggtgggtgg ctgatccccg agtccgagct
     1741 cgaggagcgt ttcgacacct cgggaggtcc cggtggtcag cacgccaacc gcaatgagac
     1801 tgctgtcact ctgcggttca gcatcgccga ttcgtcactc ccacccgacc tgcaagagag
     1861 gctgatcagg cgcctcgggt catgggtcga agtgaatgcg gctgatacca ggtctcaatg
     1921 gcgcaaccgc gaagtcgccc gggaaaggct catcgccaag ctcgaagacg ctctcgttgt
     1981 gcccaagcct cgcaagaaga ccaagccgtc gaaagccgcc aaggaaaggc gtttggagac
     2041 caagaaggcg cggagcgacg tcaagaagaa gcgacgtccg cccgaggtcg actgatctcg
     2101 acgctcattc aaacgaagat gatctgggcg ccctcggaga tctcgatgaa gtctgccgcg
     2161 ctgatgatcc cctcgacccg ctcgtagaga tcctcttcct tgatgtgcat catgtcggca
     2221 gacatccggc atgcccatag atggccgccg gcgtcggtga tcatctccag gaactcggga
     2281 acgcccggga cgtcgatccc ctccatctgc ttcttcatca tgtgggtggc gaacgaggtc
     2341 atccccggga gccccatgag catcggcgac atgtgagtcg ccgggttccc caccggagag
     2401 aacttgagat gggccatcgt ctccttgttg atcatgtcga agccccaaaa ggtgaagaag
     2461 acatgggtct cgattccttc acccagggcc gcatttgcca gcacgaggcc gggatatgcc
     2521 atgtcgagtg atcctttaga gcagatgatt gccagcttcc ggtcggttga ctcgtcgtcg
     2581 aacaccggta ccggtgcttc cagtgtcatg gtcatggtgt ctccttcaga cgcatccgac
     2641 cggcttgggg agcccggcca cgtaggccag cttctttgcc ggcttcttgg ggaacagttc
     2701 gaacatttcc ttggtgggaa cgcctccggc cgtcgccatg cgccggagcg tcggtgcctc
     2761 gccgcttgcg gcgaagtccg accgggcgaa gcggattgct gcccaatggc gctcggtcag
     2821 ctccagactg atgttggagg caagggtctc ggccagaccc tcatcccact cactcgactc
     2881 ggtgagaaac ccttcggcat cgacgtggag ctcacgtccc cgcatggttg caaccgtcat
     2941 atggtcacgt cctctcttgg tggttgggcg gtaggtatct ccttgccggc catcgacatc
     3001 gcgttgggga ccggaagtcg ccgacccgga agcagcaggt gccaatagat gggcctgaag
     3061 gcgagcttgc caagatggtt gatcctcgtt tccttgagaa ggctgagagg cccgacgcct
     3121 ggaacgggat aggtgccagg caatggctcg gtctcatagt tgaagtcgat gagcaatgcc
     3181 ttgcccgagc ccgactcgac gaagcaattg gcgtgcccat cgaacgaccg ctccatcggg
     3241 cgaccggcga tgtgatcaat gaagttctcg acgaacacgt cgaccgcaaa atgagcgacc
     3301 gaccctgcct tggagatggg caaggccgcg gcatcgccta tggcgaatac gttgtcgcag
     3361 ccgtccgcaa ggaatgtgtg cggatcgacc ctgacatggt tcagctcgtc gcccaggccc
     3421 gatccggcaa cgaagtccgc ccccatgttg accggaaccg tgaccagaag atcgaagggg
     3481 atgacccgct cgtcgtacga gacgagagca tggtcgtcga tccgctccac gtagaagtcc
     3541 ggttccacat ggatgtcccg ggcagcaagc atgtcgccaa gctgtgctga ggcgattggc
     3601 ctggtgaagg caccggacag cggggtggcg aaggtcatct cgacccgatc tcgaatcccg
     3661 atctgtctga agtagtcgtc ggccaagaag gcgaattcca gtggcgcgac cgggcacttg
     3721 attggcaact ccgtgatgtg gaccacaaat cgtccaccgt cccaaagcgc caacttccag
     3781 cgcagagcct cagccccggc catcgtgtag aactcgtgca cagtgtcccc gagctcggga
     3841 ccatccattc caggtgtcat ctccggtcgg gggtgggttc cggtcgcaat taccagctgg
     3901 tcgtaatcga ggtttcgacc accggccaga tgaacccggt tggattcgac atcgacccgc
     3961 ttcaccggct cagtgaccag ttcgactccg tcggggagga gtgctcgggt gggcttgcgg
     4021 aggtcatccg actcgtagcg accgaagggg acgaacaaca gacccggttg atagagatga
     4081 gagacagcgg gctcgaccac ggtgatctcc cactcttctt tcgggagttt tggccggagt
     4141 ttgttcacga tcatggtgcc ggccgtgccg gcgccgagaa caacaagcct cttcaacggc
     4201 tcaacctttt cataacccaa acgctacgaa cagcccgacg agccatctag ggccgaagga
     4261 caccgcgcct ggcaatttgg accgtattgc cgtggcgatc cgggcatcaa tcgtcgtttc
     4321 tctggtgccg actcagagcc attggacatt gtgaccttgg gcactggcgc caactcgccc
     4381 ggtaacggac gattgagcca tgagaactgg aaatcgtgtc gccctggtcg cggcgctcgc
     4441 ccttgcggtc gcctcttgcg ggagcgacgg gagcacaaca acaatcgaca ggacaaccag
     4501 cacggtgccg gaaaccacca tcagcctcac cgcacctgcg accaccacaa cgatcagctc
     4561 gaccagcaca tcggcggcca ccgccggaag gacggtctac ttcttgttgg atgaggtcgg
     4621 tgggagctct ggtgttggcc cgttcctggt cccggtctat cgcgaggggc ctgagtcgga
     4681 cgacgcggct tcggatgctg tagagctctt gctggcagga ccgaccgcag gtgaaatggc
     4741 tgggaccccg gcgatgcaaa ctgccatccc cgaggggacc tcactgctcg ggctgagtgt
     4801 cgatgacggt gtcgccacgg ttgacctcag tgccgagttc gatgacggcg gtggcagtgc
     4861 gggcatgttc gcacgccttg ctcaggtcgt gttcaccgtg acgcgcgcca cgggggtcga
     4921 tgaggtgact ttcgaactgg cgag
//
LOCUS       DBNHXU010000272         2955 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4651054, whole genome shotgun sequence.
ACCESSION   DBNHXU010000272 DBNHXU010000000
VERSION     DBNHXU010000272.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4651054"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..86
                     /locus_tag="AC161X_06840"
     tRNA            13..86
                     /locus_tag="AC161X_06840"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:47..49,aa:Met,seq:cat)
     gene            307..1572
                     /locus_tag="AC161X_06845"
     CDS             307..1572
                     /locus_tag="AC161X_06845"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4989302.1"
                     /translation="MSARGRFGRVWERVLSLGSYQEEPETQQGKRRIIVGVSVVFVMF
                     NLMAGFGELGAGRGWLAVTAWLTVAANVTAMTFLRFKPRWFVGVLNALFFFLSVVGLV
                     ETVLLGGLLQSSAQMLWGLLTVLGALIALNLRSAFAWLVVFTVSVIAAAWLPTWIGGI
                     YTVEASAAELAINICGVTVLVFATTAYFVRQRDRFQRQSDDLLHNILPHEIAKRLKGE
                     NKMIADHFESASVLFADVVDFTPMSAGMSPAELIGLLNEVFSAFDRFVDELGLEKIKT
                     VGDEYMVASGVPVERPDHAESLAELALRIRDFVASNTFNGHKIELRIGIHSGPVVAGV
                     IGIHKYAYDLWGDSVNTASRMESEGLTGSIQVSATTYELIRDSFVCEPRGVVSVKGKG
                     DLFTYFLVGRHADAASARLPAYRNDSSLL"
     gene            1700..2116
                     /locus_tag="AC161X_06850"
     CDS             1700..2116
                     /locus_tag="AC161X_06850"
                     /EC_number="5.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF019013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030638 - polyketide metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ester cyclase"
                     /protein_id="HIR4989303.1"
                     /translation="MSQENKELVRRWFEALSRHDADSVADMVTIDFVHNSSTNQGRDG
                     VRAELDYWFSAFPDAAVSIEDLIADGDRVVARVTSSGTHGGEFMGSPATGKRVSVQEV
                     DIFRIENDKIAEAWAAVDFFGMLTQLGLLPSEEPVG"
     gene            complement(2149..>2955)
                     /locus_tag="AC161X_06855"
     CDS             complement(2149..>2955)
                     /locus_tag="AC161X_06855"
                     /inference="COORDINATES: protein motif:HMM:NF024824.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HIR4989304.1"
                     /translation="RARLAELRISQGRFEEAERLLSGIESRPESIRPVVALHMERGEL
                     APAASVLHRRLRKMGSESVGAVPFLAMLVTVQIARGDIDGAISSAHSIERLAKRTGDD
                     RNLAESDLATGRVLLAQGNATEAVEALSSAVERFDRLKMRLDSGRARLDLGEALAATG
                     ETELAVNEVRAASAGFEKIGAVRQADKADALLRSLGERGRVGPKGVGRLTRRELEVLE
                     LLSEGLTNAEIAQRLFISTKTAGNHVSNILMKLNLRSRTEAVAFALRPRS"
BASE COUNT          521 a          874 c          908 g          652 t
ORIGIN      
        1 ctattccatc gacgcggggt ggagcagtct ggtagctcgt cgggctcata acccgaaggt
       61 cgcgggttca aatcccgccc ccgctactca ggaaaaccca ggcaccgcct gggtttttct
      121 acgtctaagt ccggccgtgg aggagcccat ggcggacgcc atcccacatc catcccgcag
      181 gagcgccgat tcctggtccg cccgacctcg accaagtccg acctcatcgt gctgcatcga
      241 cactttcggt agacgctcgt aaaccctttc gaccattgta gaaacgctcg caagtatcgt
      301 gattggatgt ctgccagggg tcgctttgga cgggtatggg agcgtgttct gtcccttggc
      361 tcctaccaag aggagcctga aacccaacag ggaaaacggc gaatcatcgt gggcgtgtcc
      421 gtcgttttcg tgatgttcaa tctgatggcg ggcttcggcg aactcggtgc tgggcggggg
      481 tggctggcag tcactgcttg gttgacggtt gcagcgaatg tgaccgcgat gacatttctg
      541 cgcttcaaac cacgttggtt cgtcggggtt ctcaacgcct tgttcttctt cttgtctgtt
      601 gtcgggcttg tggagactgt cttgctcggt ggtctgctgc agtcctcggc ccagatgttg
      661 tggggcctac tgaccgtgct cggcgctctc attgcgctca acctgcgatc ggcgtttgca
      721 tggttggtgg tcttcaccgt cagcgtgata gcggcggcgt ggctgccaac ctggattggg
      781 ggcatctaca cggtcgaggc gtcggcggct gagttggcga tcaacatctg cggagtgacc
      841 gttttggtct ttgcgacgac ggcgtacttc gttcggcagc gcgatcgctt ccagcggcaa
      901 tccgacgacc tgctccacaa catcctcccc catgagatcg ccaagcggct gaaaggcgag
      961 aacaagatga tcgccgacca cttcgagtcg gcctcggtgc ttttcgccga cgtggtcgat
     1021 ttcaccccga tgtcggccgg gatgagcccg gctgagctca ttggtctcct gaacgaggtg
     1081 ttctccgctt tcgatcggtt cgtcgatgag ctgggcttgg agaagatcaa gactgtgggc
     1141 gatgagtaca tggtggcctc gggagtgccg gttgagcgac ccgaccatgc cgaatcgcta
     1201 gccgagttgg cgctgcggat ccgcgacttc gttgcttcca acaccttcaa tggccacaag
     1261 atcgagctgc gaatagggat ccactcgggg ccagttgtcg ccggggtcat cgggattcac
     1321 aagtacgcct acgacctctg gggggattcc gtcaacacgg cgagtcgcat ggaatcggaa
     1381 ggacttaccg gaagcatcca ggtcagcgcg accacatacg agttgatccg cgacagcttc
     1441 gtttgcgagc ctcgcggcgt cgtttcggtc aagggcaagg gcgacttgtt cacctacttc
     1501 ctggttggcc ggcatgccga cgcggcgagc gcgcgtttac cggcctatcg caacgattcc
     1561 tctcttctgt agcgaatgct ggcccttctt cgctggctcc acgacggctg agaaatggtc
     1621 gccgaaacgg gtgattctcc ccatgcttct cgtccgtcaa ttcggcacgc tgtcaggatc
     1681 cgagacgaaa ggagatcaag tgtctcagga gaacaaggag ttggttcgca ggtggtttga
     1741 ggcgctgagt cgtcatgatg ccgattcggt tgctgacatg gtgaccatcg atttcgtcca
     1801 caacagttcg accaaccagg gtcgcgatgg ggttcgagcg gagctcgact actggttctc
     1861 ggcattcccc gacgccgccg tatcaattga ggatctcatc gcggacggtg accgtgtcgt
     1921 agcaagggtc acctccagcg gaacccatgg cggcgagttc atggggtcgc cggctacggg
     1981 aaagcgggtt tcggtgcagg aggttgacat cttccgcatt gagaacgaca agatcgccga
     2041 ggcctgggcg gcagtcgact tcttcggcat gctcacccag cttggcttgt tgccttcgga
     2101 agagcccgtg ggttgacctc ctgattggcc taagtggaca acgaggtgtc aggatcgagg
     2161 ccgcagcgca aatgctacgg cctcggtccg gcttctcagg ttcagcttca tcagaatgtt
     2221 gctcacgtgg ttgccggcgg tcttggtgct tatgaaaagg cgttgtgcga tttcggcgtt
     2281 ggtaagaccc tcggagagaa gctccagaac ctcgagttct ctccgtgtca gacgtccgac
     2341 gcccttggga ccgactctgc ctcgttcgcc caacgatcga agcagagcat ctgccttatc
     2401 tgcctgacgg accgccccta tcttttcgaa tccggccgag gctgctctga cttcgttgac
     2461 tgcgagctcg gtttccccgg tagctgcgag cgcttcgccg agatcgaggc gggctcgtcc
     2521 cgagtcgagc cgcatcttga gacgatcgaa gcgctcaact gcggacgaaa gcgcctcgac
     2581 agcctcggtc gcgtttccct gcgctaggag gacccggccg gtcgccaaat cgctttcggc
     2641 caggttccga tcgtcaccgg tgcgcttggc cagtctctcg attgaatgcg ccgacgagat
     2701 ggcaccgtcg atgtcaccgc gggcgatctg aacggtgacc aacatcgcca ggaacgggac
     2761 agcaccaacc gattctgacc ccatctttcg cagccgccgg tgaagcaccg atgccgcagg
     2821 tgccagttcg cctctctcca tgtgcagtgc aaccaccggg cggatcgact cgggccttga
     2881 ctcgattccg gacaggagcc tttcggcctc ttcgaagcgt ccttgggaga tcctcaactc
     2941 cgccaggcga gcacg
//
LOCUS       DBNHXU010000273         2105 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2272122, whole genome shotgun sequence.
ACCESSION   DBNHXU010000273 DBNHXU010000000
VERSION     DBNHXU010000273.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2272122"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2023)
                     /gene="gcvP"
                     /locus_tag="AC161X_06860"
     CDS             complement(<1..2023)
                     /gene="gcvP"
                     /locus_tag="AC161X_06860"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase"
                     /protein_id="HIR4989305.1"
                     /translation="MPNPRERFADRHIGPSPDDIEKMLAAVGAGSLDDLIAQAVPEPI
                     LDRTLELPPAMTEPEVIIRLGEIAAKNQVLPSLIGLGYHDTITPPVIQRNVLESPSWY
                     TAYTPYQAEISQGRLEALLNFQTMVTDLTGREIANASLLDEPTAAAEAMAMCRRLDKR
                     DRDVFYIDDDCHRPTVDVVKTRARAIGVEIRMLSEGVESGAAFGALLQYPGSSGEVPD
                     HRELVGELHADGVIVAVATDLLALTLLTPPGEWGADIVVGSAQRFGVPLGYGGPHAAF
                     IATREEMVRVLPGRLVGVSSDRAGNIGYRLALQTREQHIRRERATSNVCTAQVLLAVI
                     AGFYACWHGPEGLKDIASETHSLTSRLVRSLRSGGAEVVNETWFDTLTVRLESADAAI
                     DAALRVGVNLGRVDERHVRIALDETTSPDLIERVLGALGFNDLESEGDSGIPGPLRRE
                     SEFLTHPVFNSHHSEHEMLRYLRRLADRDLALDRSMIPLGSCTMKLNATTEMIPITWP
                     GFARIHPYAPASQAGGYRELIRELESDLCAITGYDVVSFQPNAGSQGELAGLLAIRAF
                     HSSRGEEHRDVCLIPASAHGTNAASAVMAGMKVVVVATDDQGNIDLADLRSRVDAAGD
                     HLAAVIVTYPSTHGVFEEGVLELVGIVHEAGGQVYVDGANLNAMVGLARP"
BASE COUNT          387 a          666 c          672 g          380 t
ORIGIN      
        1 ccggccgggc cagtccgacc atggcgttca gattcgcccc gtcgacatag acctggccgc
       61 cggcctcgtg gactatccca acgagctcaa gaaccccttc ctcgaagaca ccgtgcgtcg
      121 acggatatgt cacgatgacc gccgcaagat gatcccctgc cgcatccacc cgcgacctga
      181 ggtcagccag atcgatgttt ccctggtcgt cggttgccac gactacaacc ttcatgcctg
      241 ccatcaccgc ggaggcggcg ttggtcccat gcgccgatgc cgggatgaga cagacatctc
      301 ggtgctcctc gcctcgactc gagtgaaagg cgcggattgc caggagcccg gctagctccc
      361 cctgggagcc ggcgttgggt tgaaaggaga cgacgtcgta cccggtgatg gcacaaagat
      421 cggactccag ttcgcggatc agctctcgat acccaccggc ttgagaagcg ggggcgtagg
      481 ggtgaatccg tgcgaaaccg ggccaggtga tcgggatcat ttccgtggtg gcgttgagct
      541 tcatggtgca tgaaccgagc gggatcatgc ttcgatcgag ggccaggtca cggtcagcca
      601 accggcgcag atagcgaagc atctcgtgct cggagtgatg actgttgaac acggggtggg
      661 tgaggaattc ggactcccgt cgcagcgggc ccgggatgcc ggagtcgcct tccgattcga
      721 ggtcgttgaa tccgagggca ccgagcaccc gttcgatcag gtccgggctg gttgtctcat
      781 cgagagcgat ccgaacatgc ctctcatcga ccctccccag gttcacacca acacggaggg
      841 cggcatcgat tgcggcgtca gcgctctcga gacgaactgt caaagtgtcg aaccaggtct
      901 cgttgaccac ctctgcgccg cccgagcgca aagatcgaac gagtctcgat gtgagactgt
      961 gggtctcaga ggcgatgtcc ttcaggccct ccggcccatg ccagcatgca tagaacccgg
     1021 cgatcacggc gaggagcact tgagcggtgc acacgttcga cgtcgcccgc tcgcgacgga
     1081 tgtgctgctc tcgggtctga agcgccagtc ggtagccaat gttcccggcc cgatccgatg
     1141 agactccaac cagcctcccg ggaaggaccc ggaccatttc ctctctggtg gcgataaatg
     1201 cagcatgggg cccgccatag cccaggggga caccgaacct ctgcgccgac ccgacaacga
     1261 tgtctgcccc ccactccccc ggaggggtga gcagggtcag cgccaagagg tcggtggcca
     1321 cagcgacgat gactccgtcg gcgtgcagct caccgaccaa ctcgcggtga tcggggacct
     1381 caccggacga gccggggtac tggagcagtg caccgaaggc ggctccggac tcgacgcctt
     1441 cggaaagcat cctgatctcg accccgatag ctcgcgccct ggtcttgacc acgtcgaccg
     1501 tcggtcgatg acaatcgtcg tcgatgtaga acacgtcacg atcccgctta tccagacgtc
     1561 gacacatcgc catcgcctcg gctgcggcgg tgggctcgtc gagaagggag gcgttggcga
     1621 tctcgcgccc ggtgagatcg gtgaccatgg tctggaagtt gagaagggcc tcgaggcgac
     1681 cctgggaaat ctcggcctga tacggggtgt acgccgtgta ccacgagggg ctttcgagga
     1741 cattgcgctg gatcacgggc ggagtgattg tgtcgtgata cccgagcccg atcagcgacg
     1801 gcaatacctg gttcttggct gcgatttcgc cgagccggat gatcacctcg ggctcggtca
     1861 ttgcaggcgg aagctccagc gtcctgtcga gaatcggctc cggcactgcc tgtgcaatga
     1921 ggtcatcgag gctgccggcg ccaaccgcgg ccagcatctt ctcgatgtcg tcaggactgg
     1981 ggccgatgtg gcggtcggcg aagcgctctc tggggttggg catcagtctc ctctaaggct
     2041 gtcagccctc ccccgctgtc gtcatctcca gcggcgcctc gatcgaatgg tccacgtgcc
     2101 tgaga
//
LOCUS       DBNHXU010000274         2521 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4455954, whole genome shotgun sequence.
ACCESSION   DBNHXU010000274 DBNHXU010000000
VERSION     DBNHXU010000274.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4455954"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..884
                     /locus_tag="AC161X_06865"
     CDS             132..884
                     /locus_tag="AC161X_06865"
                     /inference="COORDINATES: protein motif:HMM:NF016176.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HIR4989306.1"
                     /translation="MKRILGWTLAAILVLGVGLVGWLYFAGGSGEPSTELTTPPIAEA
                     STTTASESTDPTADPVTTVPSTVTSAGQAFVIDPSLSVASFEIDEVLRGSPKHVVGTT
                     SELAGQIQVDPADLTSAEFSQILINARTFETDSGNRDRAIRGPVILNSASDEFEFISF
                     DVTSVDGLSGAAAVGEPIDFTVTGDLKIKDTTQSVTFDVSATFIDENTIEGSAQTTVL
                     RDDFGLGIPQAPGVADVSQEVVIRLDFVATSV"
     gene            918..>2521
                     /gene="dnaG"
                     /locus_tag="AC161X_06870"
     CDS             918..>2521
                     /gene="dnaG"
                     /locus_tag="AC161X_06870"
                     /inference="COORDINATES: protein motif:HMM:TIGR01391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HIR4989307.1"
                     /translation="MADRSDIDRVREATDLVELISEVTTVKRSGRSFMAVCPFHEEKT
                     PSMSVDRARGLYHCFGCGKGGDVFTFVSETQGVEFGDALELLARKAGITLTQDPAEAR
                     NRGRRGAAIEAIRRAIEVYNQRLRTAADAGPARSYLRGRGYDGDLIDEWKMGFAGVDW
                     DTLTKELKAGGVEDQVLIDAGLARRGRNGVFDVFRGRLMFPIYDLRGDPVGFGGRKLE
                     GIDRKVTNNPDAKYVNSADSLLYHKGSLLFGLDRARKNMSEDSPAVIVEGYTDVIAMH
                     QAGITTAIATCGTALGDGHFDLLRRFSERVVLAFDADEAGSRAALRGDQLETPFRLDL
                     DLRVAVMPDGLDPADLVQEGRGEELVSAVNSARPLLEHRIDHEVAKYDLAGPEGRARA
                     LHSAVAELRKVTDVIARREYMRYVARRVGVDLADVEAAVGGGRTTGTRGGTSTDKPFD
                     RIDVELLRLVLANNGGLSDLTESDFADERLGEAFAALESRISSTPHGTPLDISDVEPL
                     LQGMLLSLALDDRPLASVGEMNGRLK"
BASE COUNT          440 a          775 c          829 g          477 t
ORIGIN      
        1 cgcgaccggg acaccatggc ccaggaccgg atcccgatcg caggtttggt cgactatctg
       61 gcgggaaaac tctcctgagc tttacccggg cttgatcccg ggcgctcaca cttcactcag
      121 gtggcaagac gatgaaacgc atccttggtt ggactctggc agcgatcctg gttctcgggg
      181 tcggtcttgt gggctggctc tacttcgccg gcggctcggg cgagccttcg acggagctga
      241 cgacgccgcc gatcgcagag gcgtcgacca caaccgcatc ggaatcgacc gatcccaccg
      301 ccgacccggt cacaacggtc ccctcgaccg tgacctcggc cggccaggcc ttcgtgatcg
      361 acccttctct gtcggtagcc agcttcgaga tcgatgaggt tctccgtggg tcgcccaagc
      421 acgtcgttgg aaccacgtcg gagttggccg gtcagatcca ggtcgaccct gccgacctca
      481 cttccgccga gttctcccag atcttgatca acgctcgcac attcgagacc gactcgggaa
      541 accgggatcg cgccatccgg ggcccggtga tcctgaactc cgctagcgac gagttcgagt
      601 tcatcagctt cgatgtgact tcggtggacg gactcagcgg ggcggcggcg gtcggggagc
      661 ccatcgactt cacggtgacc ggagatctca agatcaaaga cacgactcag tcggtgacct
      721 tcgacgtctc ggccacgttc attgacgaaa acacgatcga gggaagtgca cagacaacgg
      781 tgctccgaga cgacttcggc ctgggtattc cccaggctcc gggcgtggcc gatgtctcgc
      841 aggaggtcgt gattcgcctc gattttgtgg ccacctcagt ctgaagacca acagggcgtc
      901 cttatgatcg gtaggccatg gcagatcgct ccgatatcga ccgggtccgg gaggccactg
      961 atctcgtcga actgatatcc gaggtcacca ccgtcaagcg ttccggccgc tccttcatgg
     1021 cggtttgccc cttccatgag gagaagacgc cgtcgatgtc tgtcgaccgg gcccgcggcc
     1081 tctatcactg cttcggatgt ggcaaaggtg gcgacgtctt cactttcgtt tccgagaccc
     1141 agggtgtgga gttcggggac gcccttgagt tgttggcccg caaggccggg atcactctca
     1201 ctcaggatcc ggccgaagcc cgcaaccggg ggaggagagg ggcggccatt gaggccatcc
     1261 gaagggcgat cgaggtctac aaccagcggc tgcgaactgc ggccgacgcc ggcccggcgc
     1321 gctcttatct gcgtggccga ggctacgacg gcgatctgat cgacgaatgg aagatgggtt
     1381 tcgccggtgt cgactgggac acactgacca aggagttgaa ggcgggcggc gtcgaggatc
     1441 aggtgttgat cgacgccggc cttgcccgtc ggggccgaaa cggggtcttc gacgtctttc
     1501 gaggccgtct gatgtttccg atctatgacc ttcgtggcga cccggttgga tttggggggc
     1561 gcaagctgga ggggatcgac cgcaaggtga ccaacaaccc cgacgccaag tatgtgaatt
     1621 cggccgattc cctcctgtat cacaagggat cgctcctgtt tgggctggat cgtgcccgca
     1681 agaacatgtc cgaagactcg cctgcggtga tcgtcgaggg ctacaccgac gtgatcgcca
     1741 tgcaccaggc ggggataacc actgcaatcg caacctgtgg gacggcgctt ggtgacggcc
     1801 atttcgatct gttgcgacga ttctcggagc gggtggtgct cgccttcgac gccgatgagg
     1861 ccggctccag ggctgccctc cgcggcgacc agttggaaac acctttccgc ctcgacctcg
     1921 atctccgggt ggcggtgatg cccgatgggc tcgaccctgc cgatctcgtg caagagggcc
     1981 gtggcgagga gttggtgtcg gcggtgaact cggcccgccc gctgctcgag caccggatcg
     2041 accacgaagt ggccaagtac gacctcgcag gccccgaggg tcgggcccgg gcattgcaca
     2101 gcgctgtggc ggagctccga aaggtcaccg atgtcatcgc tcgccgtgag tacatgcgct
     2161 acgtggcgcg gcgggttggg gtggacctgg cggatgtgga ggctgcggtc ggcggggggc
     2221 gaacaaccgg gacaagaggg ggtacttcga ccgacaagcc gttcgatcgc atcgacgtgg
     2281 agctgctccg tctggtcctg gccaacaacg gcgggctctc agatctcacc gagagcgact
     2341 tcgccgatga gcggctgggc gaggcctttg cggccctcga atcccggatc tcgtcgacac
     2401 cacacggcac gcctttggac atctctgacg tcgagcctct gttgcaaggg atgctgcttt
     2461 cccttgctct cgatgatcgg cccctggcat cggtcgggga gatgaacgga cggctgaagg
     2521 a
//
LOCUS       DBNHXU010000275        13396 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5051382, whole genome shotgun sequence.
ACCESSION   DBNHXU010000275 DBNHXU010000000
VERSION     DBNHXU010000275.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 13396)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13396)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13396
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5051382"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..451)
                     /locus_tag="AC161X_06875"
     CDS             complement(<1..451)
                     /locus_tag="AC161X_06875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989308.1"
                     /translation="MVRRLAGLKARLTWNGVRSDVQRRFGLPIALILLSWLGLWLAST
                     HLETARALQAESAEAFASYMGWVALFTFLAWVALPVVIFPLDENLDPQQLALLPITSN
                     QMISGLVAASFIAPSTVIPLLLLGANAAALGAGWWMIIPASLAFLGLL"
     gene            complement(436..1239)
                     /locus_tag="AC161X_06880"
     CDS             complement(436..1239)
                     /locus_tag="AC161X_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007454129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989309.1"
                     /translation="MTQPLVDESATESLEAPPAVWAKGLVRRFGKTVAVDHVDLSVGP
                     GEFYGLLGQNGAGKTTTIKTIVGLLRPNEGSVGVGEFDTWESPREVKARIGVLPEEFN
                     LYERLTGAELLDFTAAMHGLSREEATRRRDELLDLLDLLDEGSKLIGDYSRGMRKKIA
                     LGAAIIHRPPVLFLDEPFEGVDAVSARLIRNLLLRAAQGGTTIIFSSHEMHLVEKLCT
                     RIGIMVQGRLTIEETPEGLCRLMGVDNVEDAFIAAVGGKTPDDSSWYAG"
     gene            1346..3010
                     /gene="dnaX"
                     /locus_tag="AC161X_06885"
     CDS             1346..3010
                     /gene="dnaX"
                     /locus_tag="AC161X_06885"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HIR4989310.1"
                     /translation="MEYQALYRKYRPQSFDDVIGQSHVTTTLAREIADGRVAHAYLFT
                     GPRGTGKTTTARILAKALNCTNRGPQGAPCNECVSCVSITEGTSLDVLELDAASHNSI
                     DDIRDIRLSVTTVASTATSKRVFVLDEAHMLSKAAGNALLKTLEEPPEHVHFVLATTE
                     PYKLLDTIRSRSQRFDFHPVPIEDLAGHLSLIAEREGYQFDAAALLSIARHAGGSVRD
                     SLSLLEQVAALGAGTVEVGGVRRALGLADSEAFMRLVEAVAAQDAKAALELVAELSAD
                     GVDLRRFVAESVAFFRGAFLAHYAPNLAEIADEPPEVYGAWRRAAELVTSGDVLRAVD
                     ILGEALIRLREGREERLMTELALIKLTRPETASDTTALLSRIERLERGQPRPVVAESP
                     SVAAEKTPSIDAAGESVEPASDTPAVSAIPAGNGSGISFEQLQKVWPGLFGSLRDVLG
                     ARRWAFFREAVPVAVEGDTITLEVAHDFHLQSLQEDDVVSSIVATRAGDLLGSEVKIQ
                     FRSRQGTGTEAESEEIDLGDLEERSERSTDPTSLLVDQLGAEEVEE"
     gene            3032..3355
                     /locus_tag="AC161X_06890"
     CDS             3032..3355
                     /locus_tag="AC161X_06890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HIR4989311.1"
                     /translation="MNRQPDMRQLMKQAQQMQAQLAAAQAELAQRTFVGSSGGGMVTA
                     TVTGGQELVAISISPEVVDPDDIEMLEDLVLTAVRQGFEAAAAAASEELGGLTGGLDL
                     GGILG"
     gene            3355..3948
                     /gene="recR"
                     /locus_tag="AC161X_06895"
     CDS             3355..3948
                     /gene="recR"
                     /locus_tag="AC161X_06895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011290491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HIR4989312.1"
                     /translation="MFEGPVQRLIDELGRLPGIGQKTAQRLAFHLLAVEEADARRLAT
                     AIVDMREQVGLCSRCFNVAAGEECAICRDSRRDVTLVCVVERPQEVMVVERTQEFRGR
                     YHVLGGSLSPINGVGPGQIRIEELKARVGPEGITELILATNPTVEGDATAMYIARELK
                     PLGVKVTRLASGLPVGGDLDYADELTLGRALAGRMEL"
     gene            3945..4763
                     /locus_tag="AC161X_06900"
     CDS             3945..4763
                     /locus_tag="AC161X_06900"
                     /inference="COORDINATES: protein motif:HMM:NF024948.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HIR4989313.1"
                     /translation="MRRTASGVLSALAVLALGFVVALAANSVPGSAGDEPILDLAWQP
                     GVEVESILTWLIVIAAVIGALIMVFAAKQGSPSQERKRNRLVVLVVGVLVFALIARFV
                     RSIAGTLAPETSEVVGDVVETPIGQVTGNGMWMLSLLVAAVVAIALAKVGLSIRSGAP
                     PVVGASPVEAVAPVAVGSLPSTSYGDDPRGRVFTAYADFEMALDEVGVPRSPSETAMR
                     HVGRATSALDLDAQVVAVLSGHHSDARFGPLEPTIDEAAEAESASERLRGELHQ"
     gene            4760..5215
                     /locus_tag="AC161X_06905"
     CDS             4760..5215
                     /locus_tag="AC161X_06905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989314.1"
                     /translation="MSRVLTTALLAAVVSALLLLQLQRGQELWMWELLIVFVVVWLAR
                     SLPDGGAGNERRLFETTITNPTRLPRSVSTTEMAAVDALAGSLAREQRIRPMIERIAV
                     HRLRRRGLAIESAQAMALLGEDQWRWLTGREESLRAGELEQLVSGLERL"
     gene            5212..6195
                     /locus_tag="AC161X_06910"
     CDS             5212..6195
                     /locus_tag="AC161X_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989315.1"
                     /translation="MSALPADAGTIGALAKSILDEVERAVHGKRRELELILMAILADG
                     HILIEDLPGLAKTLVARSFAAATSLQFSRVQFTPDLMPMDITGSFVLDPATGLPGFRP
                     GPVFTNVLLADEINRAPAKTQAALLEAMQERQVTADGVTRPLGPPFLVIATQNPIEYE
                     GTYPLPEAQIDRFLIRARLGYPKPDAEWEMLRRRAERGTDEVELDVVVDRETLMGMQK
                     VVEEIHVSEPVGKYIVQLVAATRSSGRVRVGSSPRGSLAVMKLARARAALSGRGFVLP
                     DDVQDVAVPALAHRLILRPEFWAQDLSEERVIEEVLEQVPSPPATPSVHAP"
     gene            6192..7478
                     /locus_tag="AC161X_06915"
     CDS             6192..7478
                     /locus_tag="AC161X_06915"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR4989316.1"
                     /translation="MKAVATPRLRAYVTAGLVGLGAALAVGDPGPALVGTALLTLAIV
                     GLATRSVPSMTARIDTAPESMVEGGEYRIALHLESDRRIGPLFVEVGLEDMDVVSTVG
                     GRLVDGSTLSIPSLDRYSEAAFTVAPLGWGSKALGPLTVYADSPLGMFELSHVVPGVQ
                     HVVVLPEESTLRRLLKPLETNLHVGELVSNRRGAGSEFADLRPYQHGDDPRSINWRVS
                     SRAQRLWVNERHPERNGDVVLLVDAQVESDTDTEWLVDRAVRMAATLLRAHARHRHRL
                     GLVTLDGMCRWVYPGMGESHRRRTMEQLMAVTPSKVLWEAAERAVARAARRPAMVIAL
                     TSLLDPNLAGLMHSLRRSGMDVSVIEMDVESVLPAPLDEARSVGRRIWALERERLRDR
                     LASEGIPITVWRGDEPADVPIARLEQWRTSWRRRLG"
     gene            7457..7966
                     /locus_tag="AC161X_06920"
     CDS             7457..7966
                     /locus_tag="AC161X_06920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989317.1"
                     /translation="MATTARLTAIASVILGLALVLSVPTAKGSGLGALVGGVGVLVLV
                     VGLLYGLTASVTAAAAAFVLQMAIISALPVVLAPPPWAQTVLIVLIVEFASASFAART
                     RQIDPIRMVVRAMGTAVVAGGVVQVLSLLLEGSEVSGVLVRALGVGALVIAGGWVALV
                     WRRSTLTDG"
     gene            complement(7974..8963)
                     /gene="glpX"
                     /locus_tag="AC161X_06925"
     CDS             complement(7974..8963)
                     /gene="glpX"
                     /locus_tag="AC161X_06925"
                     /EC_number="3.1.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012871485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042132 - fructose 1,6-bisphosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphatase"
                     /protein_id="HIR4989318.1"
                     /translation="MTEPVEHNLGLDLVRVTETAAMGAARWLGRGDKEAADQAAVDGM
                     RLHLSTIDIDGLVVIGEGEKDRAPMLFNGERVGTGKGMTVDVAVDPVDGTTLTASGIP
                     GAITVIALSAGRGSMYTSGPLVYMDKLAVGPEAAGSVDLDAPVEENLRAVAKAKGKDI
                     NDLRALILDRPRNQSYIDEVRRVGGRISLLRDGDVAGAISTAQEDTAADILLGIGGSP
                     EAVIAAAALTCMGGEIQCRLWPRSDEERTQADESGLDVTQVLTTHDLIKPDNVFFAAT
                     GVTGGEFLGAVDFRGDQVVTHSVIMRSKTRSIRYMDAYHDLARLREISALTLD"
     gene            complement(8963..10147)
                     /locus_tag="AC161X_06930"
     CDS             complement(8963..10147)
                     /locus_tag="AC161X_06930"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013874978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HIR4989319.1"
                     /translation="MSNSVITGIARTPIGKYGGALSPLHAVDLGGAAIAAAMERSAVD
                     PAMIDEVLFGHVLQAGEGQITSRQAAVLGGLPMTVPSVTINKVCLSGMSAIGLADRAI
                     RLGDSKFVVAGGMESMTNAPHVVRELRWGARIGDVTMTDVMQHDGLFCAFDKCTMGES
                     SDHKNDRLGIGRTEQDEWSARSHQRAAEAARTRGFAGEIVPVAIPQRKGDPLLVDSDE
                     GIREDATADSLGALRPAFVSGGTVTAGNASQISDGGAALVLADRSAAEALGAPILAEV
                     LSYGQIGGTDATLHERPAEALLVALKKAGLEPGDLDLVEINEAFAAVAIWSARMLDLP
                     ETKVNVHGGGVALGHPLGATGARIVVTLINALRARGGGVGAATLCGGGGQGDAIVVRV
                     AG"
     gene            10219..10629
                     /gene="mce"
                     /locus_tag="AC161X_06935"
     CDS             10219..10629
                     /gene="mce"
                     /locus_tag="AC161X_06935"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012937316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HIR4989320.1"
                     /translation="MKLLNLDHVGIAVTDLDQAIHGYQERYGAVPLYREVIAAQGVEE
                     AMIPVGGSFIQLLQPLGLDTPVGRFLERRGEGLHHVAYMVASITTALEHLKGQGARLI
                     DESPRIGGRGTQIAFVHPDDLGGTLIELVEHANG"
     gene            10622..10828
                     /locus_tag="AC161X_06940"
     CDS             10622..10828
                     /locus_tag="AC161X_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989321.1"
                     /translation="MVEGIRIKGGAGEFEAALIAVVLDHITREEDATRRTPPGGNRAL
                     PAWIRADLNDDPEIPLELVEPDHI"
     gene            10809..11291
                     /locus_tag="AC161X_06945"
     CDS             10809..11291
                     /locus_tag="AC161X_06945"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009557021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4989322.1"
                     /translation="MSQITFDRAFPMVTLELIDQINLKAVFDIDVAPSQQGFVARNAW
                     SLAQALTEPEVAWPRAIVADSVVVGFLMLEIDPNDEDDRAFFLWRLMVGAEYQGRGFG
                     SLAVAAACDEVRGRGGTELYTSWVEGDGGPGPFYLKLGFVPTGEIDDGETVGRLGLTG
                     "
     gene            complement(11281..12819)
                     /gene="murJ"
                     /locus_tag="AC161X_06950"
     CDS             complement(11281..12819)
                     /gene="murJ"
                     /locus_tag="AC161X_06950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HIR4989323.1"
                     /translation="MSRPLRMGTAAAVVAGSVLFSRVLGLAREALLASLIGVSADGDL
                     YRQAFTIPDFLNYLLAGAYLTITLIPILSRHLERGDEEGANRAFSSVFRFVIIAIIGL
                     TVVMWAAAGPLVSLMFPNAVDHDRLVSLTRLVLPAQVFLVAGALLMAVQYTHRRFVLP
                     ALAPIIYNLGIILGGLISAVVAEASPEGFLWGAVIGSAIGNFGLQWYGARNTNTRLVP
                     VPRGQSSVGEYLILALPLMVGQSIAVLDEQFVRWFGQIEEGATSALSFARQLNMVPVG
                     VIAQAAGVAAYPFLARLAARGDTKALTSTTSRAARGTVFVAAGASAALIVLARPMVRL
                     LYQWGAFTADDTELVARLLMIYALSVPAWGLHQILSRHFYAKRKMWTPVLIGTVGTVI
                     AVPVWSGLYNLAGVEGFAMASVVIMSAYALALLVAWGADSGWQAAREVGVSLVRGLLA
                     AAAAVLLALPVVNAIFGEGSLSFLGGLGAAALGGLTALAGFLVVSFLLRAPELKEITN
                     RSSP"
     gene            complement(12816..>13396)
                     /locus_tag="AC161X_06955"
     CDS             complement(12816..>13396)
                     /locus_tag="AC161X_06955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HIR4989324.1"
                     /translation="LEELRKGRNHRNELMADRLTELGMPVTVEEILEEGGAGSIGRPH
                     IAAVMMRKGYVETIEEAFELWLTSGKPAYVERPRLTPEEAIRLARESGAVPIVAHPHT
                     LGINRAHEMADLLTRLIAAGLVGLEAFCASYRRHEREGYADLARRFGLIPSGGSDYHG
                     TYKPGLSLGTGYGDLVVPDAVVDELRNHAGAA"
BASE COUNT         2327 a         4238 c         4390 g         2441 t
ORIGIN      
        1 ccagcagacc gaggaaggcc aacgatgccg ggatgatcat ccaccacccg gcgccgagtg
       61 ccgcggcgtt ggcgccaagg agaagaagcg ggatcaccgt ggaaggtgcg atgaagctgg
      121 cggccacgag cccagagatc atctggtttg aggtaatcgg aagcagagct agttgttgcg
      181 ggtcgaggtt ctcatcgagc gggaagatca caaccggcag ggcgacccag gcgaggaaag
      241 tgaacagggc aacccagccc atgtagctgg cgaacgcttc tgcgctctca gcctggaggg
      301 cgcgggcggt ttcgaggtgc gtgctagcca gccacaggcc aagccagctg agaaggatga
      361 gcgctatcgg gaggccaaac cttcgttgga cgtcgctacg gacgccgttc caggtcagcc
      421 tcgccttgag gccggctaac cggcgtacca cgacgaatcg tccggggtct ttccacctac
      481 ggcggcgata aacgcatctt cgacgttgtc gacacccatc agccgacaca acccctctgg
      541 ggtctcctca attgtcaatc ggccctggac catgatcccg atccgggtgc acaacttctc
      601 cacgaggtgc atctcgtgtg acgagaagat gatggtggtc ccgccctggg cggctcgcag
      661 gagcaggttc cgaatcaggc gtgccgacac cgcatcgact ccttcgaacg gctcgtccaa
      721 gaacagaacc gggggacgat ggatgatggc cgcgcccagc gcgatcttct tcctcatgcc
      781 ccgggagtaa tcaccgatga gtttcgaccc ttcatccagg agatcgagga ggtcgagcag
      841 ctcgtcgcga cgcctggtcg cctcctcccg gctcagcccg tgcatggctg ctgtgaagtc
      901 gagaagctcg gctcccgtca accgctcgta gaggttgaac tcctcgggga ggactccgat
      961 cctggctttc acctctctgg gtgattccca ggtgtcgaac tcgccgacgc cgaccgatcc
     1021 ctcgtttggt cgaagtagcc cgacgatggt cttgatcgtg gtcgtcttgc cagccccgtt
     1081 ctgaccgagc aggccgtaga actcaccggg ccccacagac aggtcgacat ggtcgaccgc
     1141 caccgtcttg ccaaatcttc ggaccaggcc ttttgcccag accgcgggcg gcgcctccag
     1201 cgattcagtc gccgactcgt cgacaagtgg ttgggtcacg aggccaagta tcgacggata
     1261 agacccataa ccaaagaaga atgcgccagg aacaggccac ttgcccggcg aaccggtccc
     1321 aggtaccaag taccgtggaa cccaagtgga gtatcaggcc ctctatcgga agtaccggcc
     1381 acagtcattc gatgacgtca tcggccagtc tcacgtcacc acaacccttg cccgggagat
     1441 agcagacggc agggtggcac atgcctatct attcaccggg cctcggggta cggggaaaac
     1501 gacgacagca cgcatcctgg ccaaggcgct gaactgcacc aatcgcgggc ctcagggggc
     1561 tccgtgcaat gaatgtgtga gttgcgtgtc gatcaccgag gggacttccc tggacgtcct
     1621 ggagttggac gccgcttccc acaactccat cgatgacatc cgcgacatca ggctgagcgt
     1681 gaccacagtc gcctcaaccg cgacctcgaa gcgtgttttc gtgctcgatg aggcgcacat
     1741 gctgtccaag gcagccggga acgcgctcct caagaccctg gaagagccgc cggagcatgt
     1801 gcatttcgtg ctggcgacga ccgagcctta caagcttctc gacacgattc gcagtcgcag
     1861 ccagcgtttc gactttcacc ccgtccccat cgaggacctg gctgggcacc tcagcctgat
     1921 cgctgagcgc gagggctatc agttcgatgc cgctgccctg ctgtccatcg cccgtcatgc
     1981 gggcggttcg gtcagggact cactttcatt gctcgagcag gtggctgcgc tcggagccgg
     2041 gacggtggaa gtcggcgggg tgcggcgcgc tcttggtctc gccgattccg aggccttcat
     2101 gagactcgtc gaggccgttg ctgcccagga cgccaaggcc gcgctcgagc tggttgccga
     2161 gctctcggcc gatggtgtcg atctcaggag gtttgtcgcc gagtcggtgg cgttcttccg
     2221 tggggcattc ctcgcccact acgcaccaaa tctcgccgaa atcgccgacg aaccgcccga
     2281 ggtctacggg gcgtggcgcc gtgctgccga gctggtgaca tcgggagatg tgctgcgcgc
     2341 tgtggacatt ctcggcgagg cactgatcag gttgcgtgaa gggcgggaag agcgcctgat
     2401 gaccgaactg gcgttgatca agctgaccag gccggagacg gccagtgaca ccacggccct
     2461 tctcagccgc atcgagcgac tggagcgagg ccagccgcgg ccggtcgtcg ccgagtcacc
     2521 atcggttgct gccgagaaga cgccctccat cgacgcggcc ggagagagtg tcgaaccagc
     2581 gagcgacaca ccagctgtct ctgcaatacc ggctggaaac ggatcaggga tctccttcga
     2641 acagctgcag aaggtgtggc ccggcctctt cgggagtctg cgcgatgtgt tgggtgcccg
     2701 tcgttgggcg ttcttccgag aagcggtccc ggtcgcggtc gagggggaca ccatcaccct
     2761 ggaggtcgcc cacgacttcc atctgcaatc actgcaggag gacgatgtgg tgtcgtccat
     2821 cgtcgccacc cgagccggcg atctgcttgg cagtgaggtg aagatccaat tccgctcccg
     2881 gcaggggacc gggaccgagg ctgagagcga ggaaatcgac ctcggcgatc tcgaggagcg
     2941 ctcggaacgg tccacggatc cgaccagcct tcttgtcgat cagctgggcg cggaagaggt
     3001 cgaggagtaa ctctcaagga ccctccgcat catgaatcgc cagcccgaca tgcgtcagtt
     3061 gatgaagcag gcccagcaga tgcaggccca gctcgccgcc gctcaggccg agttggcgca
     3121 gcgcactttc gtgggcagct ctgggggcgg gatggtcacc gccacggtca ccggaggcca
     3181 ggaactggtc gccatcagca tctccccaga agtggtcgac cccgacgaca tcgagatgct
     3241 cgaggatctg gttctcaccg cggtgcgaca gggattcgag gccgcggccg ccgccgcatc
     3301 tgaggagctc ggcggtctca ccggcggcct tgatctcggc ggcattctcg gttgatgttc
     3361 gaaggacccg ttcagcggct catcgatgag ctcggccggc ttccaggcat tggccagaag
     3421 actgcgcaaa gacttgcgtt ccatctgctc gcggttgagg aggccgatgc tcggcgcctg
     3481 gctaccgcca tagtcgacat gagggagcag gtcgggctct gcagccgttg tttcaacgtg
     3541 gctgcgggcg aggaatgtgc gatctgtcgg gattcgcggc gtgacgtcac tctggtgtgc
     3601 gtggtcgagc gtccccaaga ggtgatggtt gtggagcgga cccaggagtt caggggccgg
     3661 tatcacgtcc ttggaggctc tctgtctccc atcaacggtg tcgggcctgg ccagatccgg
     3721 atcgaggaat tgaaggcccg agtcggtccg gaagggatca cggagttgat cctggccacc
     3781 aatcccacag ttgagggaga cgcaaccgcg atgtacatcg cccgcgagct caaaccgttg
     3841 ggagtcaagg tgacccgcct cgcttccggc cttccggttg gtggggacct cgactacgcc
     3901 gacgagctga ctctgggccg agcgcttgcg gggagaatgg aattgtgaga aggactgcca
     3961 gcggcgtgct gagtgccctg gccgtcctcg ccctcggctt cgtcgtcgct cttgccgcca
     4021 actcggtccc cgggagcgcc ggcgacgagc cgatcctcga tctcgcatgg cagcccggtg
     4081 tcgaagtgga atcgattctc acctggctca tcgtcatcgc cgccgtgatc ggtgcgctga
     4141 taatggtttt cgccgccaaa cagggctccc cctctcagga gcgcaagcgg aaccgtctcg
     4201 tggtgctggt tgtcggcgtt ctcgtgtttg ccctcatcgc ccgattcgta cgttcgatag
     4261 cgggcaccct cgcccccgag acctcggaag tcgtcggcga cgtcgtggaa accccgatcg
     4321 gacaggtgac aggcaacggc atgtggatgt tgagccttct ggtcgcagcc gtggtcgcga
     4381 tcgccttggc caaggttggg ctatcgattc gatccggagc gcccccggtt gtgggggcct
     4441 ccccggtcga ggcggtcgct ccggtcgcgg tcggcagcct tccctcgacg tcgtacgggg
     4501 atgaccccag ggggcgggtc ttcaccgcct acgccgactt cgaaatggcg ttggatgaag
     4561 taggtgtccc caggtcgccg tcggagaccg cgatgcggca cgttggaagg gccacctctg
     4621 ccctcgacct cgatgcccag gttgttgcgg ttctctccgg acatcattcc gacgccagat
     4681 tcgggcctct cgagccaacc atcgatgagg ccgcggaggc cgagtcggca tccgagcgtt
     4741 tgcgagggga gcttcaccaa tgagccgggt cctgacgaca gcactgctcg cagccgtggt
     4801 ctccgccctt ctgctcctcc aacttcaacg cggtcaggag ctctggatgt gggagttgct
     4861 gatcgttttc gtcgtcgtct ggctggctcg tagtctcccg gacggtggcg ccgggaatga
     4921 gcgccgtctg ttcgagacga ccatcaccaa tccgacgcgt ctgccccgtt cggtctcaac
     4981 tacagagatg gcggcggtag acgccctcgc cggctccctc gccagggagc agcggattcg
     5041 cccgatgatc gagcggatcg cggttcaccg tctgcggcgt cgtgggctgg caattgagtc
     5101 tgctcaggcg atggcactcc tgggcgagga tcagtggagg tggctgacgg gcagggagga
     5161 atccctccgt gcgggagagc tcgagcagct ggtttccggg ttggagcgcc tatgagcgcc
     5221 ctacccgcgg acgctggcac gatcggtgcc ctcgctaagt cgatcctcga cgaggtcgaa
     5281 cgggccgtgc atggcaaacg gcgtgagctc gaattgatcc tgatggccat cctggctgac
     5341 gggcacatct tgatcgagga cctgcccggg ctcgccaaga cgctcgtagc ccgctccttt
     5401 gccgccgcca ccagtctcca attctccagg gttcagttca ccccggacct gatgcctatg
     5461 gacatcaccg gctcctttgt cctcgatccg gccacagggc tgcccggctt ccggcccggg
     5521 ccggtgttca caaacgtgct cttggctgac gagatcaacc gagccccggc gaagacacag
     5581 gccgctctac tcgaagcgat gcaagagaga caagtgaccg cagacggggt gacccgaccg
     5641 ctgggccctc cgttcctggt gatcgctaca cagaacccga tcgagtacga ggggacctat
     5701 cccctccccg aggcccagat cgaccgcttc ttgatccgag cccggctggg ctatcccaag
     5761 ccggacgcgg agtgggagat gttgcgccgc agggcagagc ggggcaccga cgaagtcgag
     5821 ctcgacgtgg tggtcgatcg ggagaccctc atggggatgc agaaagtcgt cgaggagatt
     5881 catgtctctg agccggtagg caagtacatc gttcagctcg ttgccgccac caggtcttcg
     5941 ggtcgggttc gagtcgggtc cagccctcgc ggatccctgg ctgtgatgaa gttggcccgg
     6001 gccagggcag ccctgtcggg tcgtgggttc gttctcccgg acgacgtcca ggacgtggct
     6061 gttcctgctc tggcgcatcg cctgattctc cggccagagt tctgggcgca ggatttgagc
     6121 gaagagcggg tgatcgaaga ggtgttggag caggtcccta gccctccggc gactccttcc
     6181 gtccacgctc catgaaggcg gtcgccactc cgcggttgcg cgcctacgtg acggccgggc
     6241 tggtgggcct aggtgctgca ctcgccgtgg gcgaccctgg accggccttg gtgggcactg
     6301 cgttgctcac cctggcaatt gttggcttgg ccacccggtc ggtcccgagc atgactgcac
     6361 ggatcgacac ggcacccgaa tccatggtgg agggtgggga gtaccggatc gcgttgcatc
     6421 tggagagcga ccgccgcatc ggaccgctgt ttgtcgaagt aggcctcgaa gacatggatg
     6481 tcgtgtcaac ggtcggcggg cgcctggtag acggaagcac tctgtcgatc ccttcgctcg
     6541 accggtattc agaggccgct ttcacggtgg cgcccctggg ctggggcagc aaggctctgg
     6601 gaccgctcac ggtttacgcc gattcaccac ttggcatgtt tgagttgagc cacgttgtcc
     6661 ctggtgtcca acacgtcgtg gtccttccag aggaatcgac tttgagacgt ctgctcaagc
     6721 ctttggagac caacctccac gtcggcgagt tggtctcgaa ccgaaggggc gccgggtccg
     6781 agttcgccga ccttcgcccc taccagcacg gtgacgatcc tcgatccatc aactggcggg
     6841 tttcgtctcg ggctcagcgc ctgtgggtca atgagagaca cccggagcgc aacggagacg
     6901 tcgtcctctt ggtcgacgct caggtcgaaa gcgataccga caccgaatgg ctggtcgata
     6961 gggctgtccg gatggcggcc accctgctca gagcccacgc caggcatcga caccgtctcg
     7021 gtctggtcac tctggacggc atgtgccggt gggtctatcc agggatgggg gagagccatc
     7081 gacggcgcac gatggagcaa ctgatggcag tgaccccgag caaggtgctg tgggaggcgg
     7141 cagagcgtgc cgtggctcgg gcagccagaa ggccggcgat ggtcatcgcc ctcacctccc
     7201 tgctcgaccc caacctcgcc gggctgatgc actcgctgcg gcgctcgggc atggacgtgt
     7261 cagtgatcga gatggatgtg gagtcggtgt tgccggctcc cctcgatgaa gcgcgatctg
     7321 ttggccggcg gatttgggct ctcgagcgtg agcgtcttcg cgaccggctt gcctctgagg
     7381 ggatcccaat cacggtatgg cgcggggatg agccggcaga cgtcccgatc gcccgtctcg
     7441 aacagtggag gacgtcttgg cgacgacggc tcggctgacg gctatcgcct cggtgatcct
     7501 ggggcttgcc ttggtcctct cggttcccac tgcaaaggga tcgggtttgg gcgcccttgt
     7561 cggtggagtc ggtgtgctgg tgttggtggt cggcctcctt tacggcctga ccgcatcggt
     7621 gaccgccgcc gccgcggcct tcgtcttgca gatggcaatc atctccgccc tcccggttgt
     7681 tctagcgcct cctccgtggg ctcagaccgt gctcatcgtt ctcatcgtgg agtttgcgtc
     7741 ggcatcgttt gcggcccgaa ctcgtcagat agacccgatc cggatggtgg tccgagcgat
     7801 gggaaccgcc gttgtggccg gtggagtggt tcaagtgctg agtcttctcc tcgaaggctc
     7861 ggaggtcagc ggtgttctgg ttcgagcact aggtgtgggt gctttggtca tcgccggagg
     7921 ctgggtggcg ctcgtctggc ggcggtcgac tctgaccgac gggtgagcct cgatcaatcc
     7981 agggtgagag ccgagatctc ccggagccgt gccaggtcgt gataggcgtc catgtatcgg
     8041 atcgagcgcg tcttcgagcg catgatcacc gaatgggtca caacctgatc gccacgaaag
     8101 tcgaccgcac ccaggaattc gccaccggtg acgccggtcg ccgcgaaaaa gacattgtcg
     8161 ggcttgatca ggtcgtgggt ggtcagcacc tgggtcacgt caagccccga ttcgtcggcc
     8221 tgagttctct cctcgtcact gcggggccac agccggcact ggatctcccc tcccatgcag
     8281 gtcagggccg ccgccgcgat gactgcctca ggggagccgc cgattccgag caggatgtcg
     8341 gcggcggtgt cttcctgagc ggtcgagatc gctccggcaa cgtcgccgtc gcgaagcagt
     8401 gaaatcctcc ctccaacccg tcgcacctcg tcgatgtacg actggttgcg gggtcggtcg
     8461 aggatcaagg cccgaaggtc gttgatgtcc ttgcccttcg ccttggcgac cgctctgagg
     8521 ttctcctcga ccggggcgtc gagatccacc gagccggcgg cctctgggcc aacggcgagt
     8581 ttgtccatgt agaccagagg gcccgaggtg tacatcgagc ctcgacccgc cgacagggcg
     8641 atcaccgtga tcgctccggg aatccccgag gcggtgaggg tggtgccgtc gaccggatcg
     8701 actgccacat cgaccgtcat gcctttgccg gtgccaactc tctcaccatt gaagagcatc
     8761 ggggcccggt ccttttcccc ttctccgatg acgaccagac cgtcgatgtc gatcgttgag
     8821 agatggagcc gcattccgtc gactgcagct tgatcggcgg cctccttgtc gccccgcccc
     8881 agccatcggg cagcgcccat cgccgccgtc tccgtgaccc ggaccaggtc caggccgagg
     8941 ttgtgctcga ctggctcggt catcagccgg cgacccggac cacgatggcg tcaccttggc
     9001 cgcctccacc gcagagggtg gccgccccaa ccccgccacc ccgggcgcga agggcattga
     9061 tgagggtgac cacgatgcgg gcgccggttg ctcccagggg atgaccgaga gccactccgc
     9121 ccccatggac attcaccttg gtctcgggga ggtcgagcat cctggcggac cagatggcaa
     9181 ccgcggcgaa cgcctcgttg atctctacca ggtcgaggtc tcccggttcg agaccagcct
     9241 tcttcagggc gaccaggagg gcctcggctg gccgctcgtg caaagtcgcg tcggtgccgc
     9301 cgatctgccc gtaggagagg acctcggcaa ggatcggtgc gcccagcgcc tcggccgcgc
     9361 tccggtcggc aaggaccagc gccgcgccgc catcggatat ctgggaggca ttcccggcgg
     9421 tgacggtccc tcccgacacg aatgcgggcc gcaaggcgcc aagtgagtca gccgtggcat
     9481 cctcccggat cccctcgtcg gaatcgacga gcagggggtc acccttcctc tgcgggattg
     9541 cgaccggcac gatctcgccg gcgaacccac gggttcgggc tgcctcggca gctcgttgat
     9601 ggctccgggc agaccactcg tcctgctcgg tgcgcccgat ccccaggcgg tcgttcttgt
     9661 ggtcggagga ctcgcccatg gtgcatttgt cgaaggcgca gaacaaccca tcgtgctgca
     9721 tgacatcggt catggtgacg tcaccgatcc gggcgcccca tcgcagttcg cgtacgacat
     9781 gaggagcgtt ggtcatgctt tccatcccgc cggcgaccac gaatttcgag tcaccaaggc
     9841 ggatcgcccg atctgccaac ccgatggccg acatccccga taggcagact ttgttgatcg
     9901 tcactgatgg aactgtcatc gggagaccgc cgaggaccgc ggcctgacgg gacgtgatct
     9961 gcccttcgcc agcctgcaac acgtgcccga acaggacctc gtcgatcatt gcgggatcga
    10021 ccgccgatcg ctccatggct gcggcgatgg ccgcaccacc cagatccacg gcgtggagcg
    10081 gggaaagagc gccaccgtat ttgccaatgg gggtgcgggc gatcccggtg atgacgctgt
    10141 tgctcacgat gacctcaggg acatgatgtt accggcgggc cggtcgcacc caaggggcgg
    10201 gtggcatagc cttggctgat gaagctcttg aatctcgatc atgtcgggat tgccgtcacc
    10261 gatctcgacc aggcgatcca tggctaccaa gagcgatatg gcgcggttcc cctctaccgg
    10321 gaggtgatag cggcccaggg agtcgaagag gcgatgatcc cggtcggagg ctcattcatc
    10381 cagttgctgc agccactcgg cttagacacc ccggttgggc gatttctgga acgacgcgga
    10441 gaagggctgc atcacgtggc ctacatggtg gcctcgatca cgactgcctt ggagcatctg
    10501 aagggccagg gcgccaggtt gatcgacgag agcccgcgga tcggaggccg gggaacgcag
    10561 atcgcctttg tccaccctga tgacctcggt ggtaccttga tcgagctcgt ggagcatgcg
    10621 aatggttgaa ggaattcgaa tcaagggagg cgccggagag ttcgaggcgg cattgattgc
    10681 cgtcgtcctc gaccacatca cccgtgagga ggatgcgacg cgacgcaccc ctcccggtgg
    10741 taatcgagcc cttcccgcct ggattcgagc ggatctcaac gacgacccgg agatcccgct
    10801 cgagctggtt gagccagatc acatttgacc gcgcctttcc catggtgact ctcgagctga
    10861 ttgaccagat caatctgaaa gcggtcttcg acatcgacgt cgcgcctagc cagcaaggat
    10921 tcgttgcccg caacgcatgg tcgttggcgc aggcgctaac cgagcccgag gtcgcctggc
    10981 cgcgggcaat cgtcgctgac agtgtcgtag tgggcttctt gatgctggag atcgatccca
    11041 acgacgaaga cgaccgggcc ttctttctgt ggcggctaat ggttggcgct gaatatcagg
    11101 gccgcggatt cgggtcgttg gcggtcgctg ccgcctgcga cgaggtgaga ggacgcggag
    11161 ggactgagct gtacacgagt tgggtcgagg gtgacggagg gccgggtccc ttctacttga
    11221 agctcgggtt cgtccctacc ggtgagatcg atgatgggga gaccgttggg cgcctcggcc
    11281 ttacgggcta gatcggttcg tgatctcctt caattccggt gctcggagca ggaaggacac
    11341 gaccaggaac ccggcgagag cggtcagccc acccaaggct gcagctccca gacctcccaa
    11401 gaagctcaga ctcccttcgc caaagatcgc gttcacgaca ggcagcgcga gcaagaccgc
    11461 ggccgccgca gccagcaagc ctcgaaccag ggagactccg acctcgcgcg ccgcctgcca
    11521 ccctgagtcg gcgccccagg cgacgagcag ggccagtgca tatgcactca tgatcaccac
    11581 cgaggccatg gcgaagccct cgacccctgc caggttgtag agcccggacc acaccggcac
    11641 cgcgatcact gtccccactg tgccgatcag gaccggggtc cacatcttgc gcttggcata
    11701 gaaatgtcgc gacaggatct ggtgcaggcc ccaggccggg acagagaggg cgtagatcat
    11761 caagagtctg gccaccaatt cggtgtcgtc tgcggtgaag gctccccatt ggtagagcag
    11821 ccgcaccatg gggcgggcaa ggacgatcaa tgcggccgat gctccggccg ccacgaagac
    11881 cgtgcctcgc gccgcgcgcg acgtcgtcga ggtcagggcc ttggtatcgc cccgggcggc
    11941 aagtcgagcc aggaacgggt aagcggctac tcccgcggcc tgggctatca cccccaccgg
    12001 gaccatgttg agttgcctgg caaagctgag agctgaagtg gcgccctctt cgatctggcc
    12061 gaaccaacgc acgaattgct cgtcgaggac ggcgatcgac tgccccacca tgagcggaag
    12121 ggccaggatc agatactcac cgactgacga ctggccgcga ggcaccggga ccaatcgagt
    12181 gttggtgttg cgggccccgt accactgcag tccgaagttc ccgatcgcag acccgatcac
    12241 cgccccccaa aggaatccct cagggctggc ttcagccacc actgccgaga tgaggcctcc
    12301 gaggatgatt cccaggttgt agatgatcgg ggccagcgcg ggaagcacga acctgcgatg
    12361 ggtgtactgc accgccatca ggagggcgcc tgccaccaag aacacctggg cagggaggac
    12421 cagcctggtc aaggagacga ggcggtcgtg gtcgaccgca ttcgggaaca tgagcgagac
    12481 caggggcccg gcggcggccc acatgacaac ggtgagcccg atgatggcga tgatgacaaa
    12541 tcggaacacc gagctgaagg cacgattcgc gccctcctcg tcgccccgtt ccaggtgccg
    12601 actcaggatg gggatcaggg tgattgtcag gtaggccccg gccagcaggt agttgaggaa
    12661 atcagggatg gtgaacgctt ggcgatagag gtccccatcc gcgctgaccc cgatcaggga
    12721 ggcgagtagg gcttcccgtg cgagaccgag gaccctggag aagaggacac tcccggcgac
    12781 gaccgccgct gcggtcccca tccgcagagg gcggctcatg cggctccggc gtgattgcgc
    12841 agctcgtcga ctacggcgtc aggcacgacc aggtcgccgt acccggtgcc aaggctgagc
    12901 cctggcttgt aggtgccgtg gtagtcactc cctcctgatg ggatcagccc gaaccgccgg
    12961 gcgagatcgg cgtagccctc cctctcgtgg cggcggtaac tggcgcaaaa ggcctccaat
    13021 ccaactagac cggcggcgat gagacgggtc agcaggtcgg ccatctcgtg tgcccgattg
    13081 atgccaagag tgtgcgggtg ggcgacgatc ggcaccgccc cggactcccg cgccagccgg
    13141 atcgcctcct ccggggtaag tctgggtcgc tcgacatacg cgggcttccc cgatgtcagc
    13201 cagagctcga aggcctcctc gatggtttcg acataaccct tccgcatcat gaccgcggcg
    13261 atgtgggggc ggccgatgct gccggcgcct ccctcttcga ggatctcctc caccgtcacc
    13321 ggcatcccga gctcggtcag ccggtctgcc atcaactcgt tgcgatggtt tcttcccttg
    13381 cgcagctctt ccagat
//
LOCUS       DBNHXU010000276         2644 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5051509, whole genome shotgun sequence.
ACCESSION   DBNHXU010000276 DBNHXU010000000
VERSION     DBNHXU010000276.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5051509"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..387
                     /gene="ilvN"
                     /locus_tag="AC161X_06960"
     CDS             <1..387
                     /gene="ilvN"
                     /locus_tag="AC161X_06960"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005948 - acetolactate synthase complex
                     [Evidence IEA];
                     GO_function: GO:1990610 - acetolactate synthase regulator
                     activity [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase small subunit"
                     /protein_id="HIR4989325.1"
                     /translation="GRSRITLVVDAPELEQLTKQLNKLVNVIKVQELEKDLALERELM
                     IARIACDPVNRGEVRDTASLFDAKVIDLGATSITFEVAGSPAHLGAFLDHMSGYGVVD
                     LVKSGRIAMRRESELSPTAATASPAS"
     gene            384..590
                     /locus_tag="AC161X_06965"
     CDS             384..590
                     /locus_tag="AC161X_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989326.1"
                     /translation="MSDHRAELQQRLSKLGISIGAAGFEAAYTRVRAVSDRAGAISDV
                     QLRSIVDDAVSGMEVLDEVAATYQ"
     gene            587..1663
                     /locus_tag="AC161X_06970"
     CDS             587..1663
                     /locus_tag="AC161X_06970"
                     /EC_number="1.1.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013906883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003862 - 3-isopropylmalate dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydrogenase"
                     /protein_id="HIR4989327.1"
                     /translation="MTAHRIAVVPGDGTGPEVVAEAVKAVDRAAQVFEFEVEYSHHEV
                     GGERYLRTGETLTGADLEALSSVDAVLFGAVGHPKVAPGILEQEIILRLRKELDLYVN
                     LRPIKLHPGVVGPLRDVDPADVDMIVVRENSEGLYVGAGEFTAKGSPFEVAVQQSVNS
                     RFGVERCIRYAFELARSRPRRHLTMCGKTNVLTYAWDLWARVFEAVAEEYPEVTTAYA
                     HVDAACLWMVEDPGRFDVIVTDNMFGDIITDLGAAIQGGMGIASGANLNPNGVSMFEP
                     IGGTAPGFEGTGQINPLAAIGAAGMMLTHLGEIEAGAAVDRAVGQVAGSLPSLRAGKM
                     EASTSEVGNRVSDLISTVVSAEAV"
     gene            1660..>2644
                     /locus_tag="AC161X_06975"
     CDS             1660..>2644
                     /locus_tag="AC161X_06975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015440847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HIR4989328.1"
                     /translation="MSRARTLFEKVWDTHVVESGDPTLLYADLHLVHEVTSPQAFASL
                     EQSGRKVRRPDLTFATVDHGIPTTGRHLGILDPLSKRQVEALRDNCARHGIVMYGIDD
                     PRQGIVHVVGPEQGLTQPGMVIVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTVWQ
                     SPPKSMSVEVTGQLPWGVAAKDLILGIITQIGVAGGTRHVIEYRGETIRGLSMEQRMT
                     ICNMSIEGGARAGMIAPDDITISFIEGRPFAPKGTDWDSAVADWESLVTDEGAIFDKV
                     VMIDASSLIPHVTWGVNPAQTVRVDDYVPSPDSFENPQQRESTVRALEYMGL"
BASE COUNT          505 a          831 c          836 g          472 t
ORIGIN      
        1 ggccgttcac ggatcacact cgtcgtcgac gccccggagc tcgagcagct gacgaagcag
       61 ctaaacaagc tggtcaatgt gatcaaggtg caggagctgg agaaagatct ggccttggag
      121 cgggagctga tgatcgcccg aattgcctgt gacccggtga atcgtggcga ggtacgggac
      181 acagcctccc tcttcgatgc caaggtgatt gaccttggag caacctcgat cactttcgag
      241 gttgccggca gcccggccca cctcggagct tttctcgacc acatgtccgg gtacggcgtt
      301 gtcgacctgg tcaagtcggg tcggattgcg atgcgacgtg agtcagagct gagcccgacg
      361 gccgctaccg cgagtccagc gtcatgagcg accatcgagc agagctccag caacggttgt
      421 cgaaactcgg gataagcatc ggcgctgccg gtttcgaagc ggcgtacacc cgggttcgag
      481 cggtgtcaga ccgcgctggc gccatttcgg acgttcagct gcgttcgatc gtcgatgatg
      541 cggtatcggg tatggaagta ctcgacgagg tggcggccac ctaccaatga ccgcccaccg
      601 cattgccgtc gtacccgggg acgggacagg gcccgaagta gtggccgagg cggtcaaggc
      661 ggttgataga gcggcacagg tattcgaatt cgaagtcgag tactcccacc acgaggtcgg
      721 cggcgagcgc tacctgagga ccggggagac tttgaccggt gcggacctgg aggcgctctc
      781 gagcgtcgat gccgtcttgt tcggagcggt cggacacccc aaggtggcac cggggatcct
      841 cgagcaggag atcatcctca gactccgtaa ggaactcgac ctctacgtca acttgaggcc
      901 gatcaaactc catccaggtg tggttgggcc tcttcgtgat gtcgatccgg ccgacgtcga
      961 catgatcgtg gtccgtgaga acagcgaagg tctctacgtc ggcgccggag aattcactgc
     1021 caaaggatcg ccattcgaag tggccgtcca acaatcggtc aacagccgct ttggggtgga
     1081 gcgctgtatc cggtatgcct tcgagctggc gcgatcacga ccgcgtcgcc atctgacgat
     1141 gtgcgggaag acaaatgtgc tcacgtacgc ctgggacctg tgggcacggg tgttcgaggc
     1201 ggttgccgag gaatacccgg aggtgaccac ggcatacgcc catgtcgacg cggcatgtct
     1261 ctggatggtg gaagaccccg gccgcttcga cgtgatcgtg accgacaaca tgttcggtga
     1321 catcatcacc gacctgggcg ccgccattca gggaggcatg ggcatcgctt cgggagccaa
     1381 cctcaacccc aacggtgtct cgatgttcga gccgatcgga gggaccgcac ctggtttcga
     1441 ggggaccggc cagatcaacc cgctcgccgc aatcggcgcc gcaggcatga tgctcaccca
     1501 tctcggggag atcgaggcgg gcgccgccgt ggaccgggcc gtcggccagg tcgccggctc
     1561 actcccctcg cttcgcgccg gcaagatgga ggcctccacc tctgaagtgg gtaaccgggt
     1621 cagcgacctg atttccacgg tcgtctcggc agaagcggta tgagccgggc ccgcaccctc
     1681 ttcgagaagg tttgggacac ccatgtggtc gagagtggcg accccaccct tctctatgcc
     1741 gaccttcacc tggtgcacga ggtcacatcg ccgcaagcat tcgcctcgtt ggagcagtca
     1801 gggagaaagg tccggcgacc cgatctcact ttcgccacgg tggaccacgg gatccccacc
     1861 acgggtcggc atctcggcat cctcgaccct ttgtcgaaac ggcaggtcga agccctgcgt
     1921 gacaactgcg cccgccacgg catcgtcatg tacgggatcg atgatccccg ccagggaata
     1981 gtccatgtgg tcgggcccga gcaggggctc acccaaccgg gcatggtcat tgtctgcggc
     2041 gacagccaca cctcgacgca cggcgccttc ggagcccttg cattcggcat cggcacctcg
     2101 gaggtcgagc atgtcctcgc aacgcagacc gtctggcaat ccccacccaa gtcgatgtcc
     2161 gtcgaagtca ccggacagct tccctggggg gtggccgcca aggacctgat cctcgggata
     2221 atcacccaga tcggcgtcgc cggcgggacc cgtcatgtca tcgagtaccg cggtgagacc
     2281 atccgcggtc tctcgatgga gcagcggatg accatctgca acatgtcgat cgaaggcggt
     2341 gcccgagccg ggatgatcgc ccccgacgac atcaccatct cgttcatcga gggacggccc
     2401 ttcgccccga agggcaccga ctgggactca gccgttgccg attgggaatc attggtcacc
     2461 gatgaaggcg caatcttcga caaagtggtc atgatcgacg cctcatccct catcccccac
     2521 gtgacctggg gcgtcaatcc cgcacaaacc gtccgggttg atgactacgt gccttcgccc
     2581 gacagtttcg agaaccccca gcagcgtgag tcgaccgtca gggccctgga gtacatgggc
     2641 ctgg
//
LOCUS       DBNHXU010000277         1719 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1481729, whole genome shotgun sequence.
ACCESSION   DBNHXU010000277 DBNHXU010000000
VERSION     DBNHXU010000277.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1719)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1719
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1481729"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..195
                     /locus_tag="AC161X_06980"
     CDS             <1..195
                     /locus_tag="AC161X_06980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020459821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989329.1"
                     /translation="VGVRAQALGRDGRLVDDFVIEEVGATLHVLNAPSPGATASLAIG
                     AHIAGLLRPQLTGLAGGRGV"
     gene            282..1535
                     /locus_tag="AC161X_06985"
     CDS             282..1535
                     /locus_tag="AC161X_06985"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008228791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR4989330.1"
                     /translation="MTDFPVATTPAPGIMGVDWEDRVDFPRLRAYRLERVRQQLEANG
                     LGAVLLFETSNIRYATATQIGYWAFNKGERFALITRKGRPRIWDFGSAAKAHRRQLPD
                     MYDLENSIGGNTGLQGAIGPSSGLHTRAAAEIAAVLRDEGLLGDPLGVDMAETAVFLA
                     LQKEGLQVTDGQQAMMLAREIKSSDEIVLLSQASAMVDGVYHEIYEALKPGIRESDIV
                     ALAHSRLFQMGSEFVEAINSIAGERCSPHPHVFSDRMIRPGDQAYFDIIHAYNGYRTC
                     YYRTFVVGRATTAQRDAFKQSREWMDAAIELVRPGNTTDMIAKVWPRAEDFGFVDEMD
                     AFGLQFGHGVGVGLHERPIISRLNSLDDPIEIKEGMYFALETYAPATDGRSAARIEEM
                     MVVTADGPQITTLFPCEELMVANEY"
     gene            1525..>1719
                     /locus_tag="AC161X_06990"
     CDS             1525..>1719
                     /locus_tag="AC161X_06990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989331.1"
                     /translation="MSTEVDSGLYDYAQRPPRLPKATKPENDRLEIDLETRIEMFEMQ
                     QVLRQFEDRVYDLFMQNLVKG"
BASE COUNT          335 a          537 c          539 g          308 t
ORIGIN      
        1 gtcggcgtgc gtgcgcaggc gctgggtcgt gatggccgat tggtcgatga cttcgtcatc
       61 gaagaggtcg gcgcgacatt gcatgtcctc aacgccccat cgcccggggc cacggcgagt
      121 ttggcgatcg gagcccacat tgcgggcctg ctcaggccgc aactgaccgg gttggcgggc
      181 ggaaggggtg tgtagtgtga ctgttcactg gtcacccagc ctgccgaggg tcgcggttcg
      241 gaaacgagat gaagactgac aggaacgagg aggagagagc catgaccgac tttccggtag
      301 ccaccactcc ggcccccggg atcatggggg tggattggga ggatcgcgtc gattttccgc
      361 ggctgcgtgc ctatcgcctg gagcgggtcc ggcagcaact ggaggccaat ggcctcggcg
      421 ctgtgctcct cttcgagact tccaacatcc gctatgccac agccacccag atcggctact
      481 gggctttcaa caagggcgag cgctttgcgc tgatcacgag gaagggccgt ccccgaatct
      541 gggactttgg ctcggcagcc aaggcgcatc gtcgacaact ccccgacatg tacgacctgg
      601 agaactcaat cggtgggaac acaggccttc agggtgccat cggtcccagc tctgggctcc
      661 acacccgggc cgctgccgag atcgcggcgg tgctccgtga cgaggggctc cttggcgacc
      721 ccttgggtgt ggacatggcg gagaccgctg tgttcctcgc actgcagaaa gagggccttc
      781 aggtcaccga cggacagcag gcgatgatgc tggcccgcga gatcaagagc tcggacgaga
      841 tcgtcctcct ctcccaggcc agcgccatgg tcgatggggt gtatcacgag atctacgagg
      901 ccctgaaacc cgggatccgt gagtccgaca tcgtggctct ggctcattcc cgtctgtttc
      961 agatgggctc cgagttcgtc gaggccatca actcgatcgc tggcgagcga tgcagccctc
     1021 acccacacgt tttcagcgac cgcatgatcc ggcccggtga ccaggcctac ttcgacatca
     1081 tccacgccta caacggctat cgcacctgtt actaccggac gtttgtcgtt gggcgagcaa
     1141 ccacagccca gcgcgacgcc ttcaagcaat cacgggagtg gatggacgcc gctatcgaat
     1201 tggtgcgacc aggcaacacc accgacatga tcgccaaagt ctggccacgg gccgaagact
     1261 tcggcttcgt ggatgagatg gatgccttcg gccttcagtt cggccacggc gttggggtcg
     1321 gcctacatga gcggccgatc atcagcaggc tcaactcact cgacgacccg atcgagatca
     1381 aggagggcat gtacttcgct ctcgagacct acgccccggc caccgacggg cggtcggcag
     1441 ccaggatcga agagatgatg gtggtcaccg ccgacgggcc acagatcacc acactcttcc
     1501 cctgcgagga gttgatggtg gcgaatgagt actgaggtcg actccgggct ctacgactac
     1561 gcccaacgcc caccccggct cccgaaggcg accaagcccg aaaacgaccg tctcgaaatc
     1621 gacctcgaaa cccgcatcga gatgttcgag atgcagcaag tgctccgtca gttcgaggat
     1681 cgggtttacg acctcttcat gcagaacctg gtcaaggga
//
LOCUS       DBNHXU010000278         4351 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2870269, whole genome shotgun sequence.
ACCESSION   DBNHXU010000278 DBNHXU010000000
VERSION     DBNHXU010000278.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4351)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4351)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4351
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2870269"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="AC161X_06995"
     CDS             complement(<1..289)
                     /locus_tag="AC161X_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4989332.1"
                     /translation="MLLEGKKLLITGVLTDDSIAWHAARIAQEEGAEIVVTGFGRGLR
                     LTERSVQRLPSECPVLELDINDPAQVEAAVADLTGRWGSIDGALHAIAYAPE"
     gene            complement(298..657)
                     /locus_tag="AC161X_07000"
     CDS             complement(298..657)
                     /locus_tag="AC161X_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989333.1"
                     /translation="MGTFHTTWGYIAVGLSGLVGLWGVVMARRPEAPKPFLWAVGVSV
                     GALLIQIVTGALVASGGVNPGNQHVFYGVVTAVTFTFAYIYRSQFQKRPTLYYGLLML
                     FCMGLGIRGIMTFGHSF"
     gene            775..1035
                     /locus_tag="AC161X_07005"
     CDS             775..1035
                     /locus_tag="AC161X_07005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989334.1"
                     /translation="MFEAVEFIEAPGQPEPSEKVVGEFADEIDAVAAARAAKVDFAMS
                     ASKAYAWWVVREQGAQLAHFIADSKSDKEFVLDLTSGQLVEV"
     gene            complement(1096..2712)
                     /locus_tag="AC161X_07010"
     CDS             complement(1096..2712)
                     /locus_tag="AC161X_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020376966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain fatty acid--CoA ligase"
                     /protein_id="HIR4989335.1"
                     /translation="MPHMQDDFPLNLSTLYDRSVRLYPDQAILSVEADRSLTRTNYGE
                     IDTRVRRLASALAGLGVSMGDAVATFAWNNRRHHELYWATANTGLICHTINIRLFADQ
                     VAYIVNHAGDRVLFVDPDLVPLLAPVVPRLETVATYVVMGPEADERIPGSIAYEDLIS
                     DAPEHGPWPELDERSPMMLCYTSGTTGNPKGVAYTQRSTYMHTISNLANIPMGPTDNV
                     LPVVPMFHASAWGYPFMAVTQGAQLTYPGPDLSAEGLVRLFENERVTFSAGVPTVWLG
                     IRQYLEAHPEADVSSIRAFLCGGSAVPRSLIEWFQTERGIRVVQGWGMTETNPVASTA
                     VLKPEMESWDLQRQLDFLETGGLPIAGLKVKIVDEVGEELPQDGVAFGELLIRGPWIA
                     AEYYKDPRSSQTFEDGWLRTGDVAKITPEGYIRITDRSKDVIKSGGEWISSIDLENAI
                     MAHPEVAEATVVGLKHVKWQERPAAFVVLREGASTDEAAIQTFLEERVATWWIPDRIV
                     FIDQIPKTGTGKFDKKVVRDQYADLLMEEA"
     gene            complement(2712..3326)
                     /locus_tag="AC161X_07015"
     CDS             complement(2712..3326)
                     /locus_tag="AC161X_07015"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR4989336.1"
                     /translation="MGYPKKLLSSGEEILTEFRPHWSRLLRQGLLTILVVVLIVLIAT
                     RRFDAKAWVITALVVVWFIVVIAEFVRWWTDKHVITNERVIHRSGLISKKGTEIPLEV
                     VNDVTFQQSAFERIVGSGDLLIESAGTHGQSSYSDIPHPEHVQKQIYEAREARMAQMR
                     TGGVPAAPPANTASQLDTLSRLHDEGKLTDEEFEAEKQKLIGGT"
     gene            complement(3343..3957)
                     /locus_tag="AC161X_07020"
     CDS             complement(3343..3957)
                     /locus_tag="AC161X_07020"
                     /inference="COORDINATES: protein motif:HMM:NF015648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR4989337.1"
                     /translation="MAYPKDLLAPGETVVYEFGSHWFALMKEALVTIAYVVLLILLVP
                     GGVNGWAFTIITLLWLWITVGGFMEWRTRENVITSERLVVRSGIMRKAGYEIPLEVIT
                     DVGFRQNLIDRAVGVGDMVIQTSGSTGQNRITNIPEPEKVKSMLVETRRNRTNSMSSG
                     RAAPPAAASPGKSNAEQLEILGKLFDEGKLTQDEYDNEKRKLLG"
BASE COUNT          834 a         1376 c         1302 g          839 t
ORIGIN      
        1 cttcgggggc gtaggcgatg gcgtggagcg ccccatcgat tgatccccaa cggccggtga
       61 ggtcggccac agccgcctcg acctgggccg ggtcgttgat gtccaactcg agcaccgggc
      121 actccgaagg cagccgctgg acgctgcgct cggtgagtcg aagaccccta ccgaatccgg
      181 tgaccacgat ctcggccccc tcctcctggg cgatcctcgc cgcgtgccag gcgatcgagt
      241 cgtcggtgag gacccccgtg atcaagagtt tcttgccttc gagcaacatg agccctccta
      301 gaagctgtgt ccgaatgtca tgatccctcg gatccccaaa cccatgcaga acagcatcag
      361 cagcccgtag tagagggtgg gtcgcttctg gaactgcgat ctgtagatgt aggcaaatgt
      421 aaaggtcacc gcggttacga caccgtagaa gacgtgctgg ttaccggggt tgaccccacc
      481 ggaagcaacc agggccccgg tcacgatttg gatgagcaag gcgccgactg aaacaccaac
      541 cgcccagagg aatggcttgg gtgcctccgg gcggcgagcc atgaccaccc cccagagccc
      601 gaccaatccg ctgagcccca cggcgatata gccccaagtt gtgtgaaagg tgcccacgtt
      661 gtgagcttag agagccggtc ccaaccgccc cgccactgtc agtggtggtt ttgaacccaa
      721 cctcctggga cagccaacta tcatgcttga gggaggaagc gagagaggtg tgagatgttc
      781 gaggcagtcg aattcatcga ggccccgggt caaccggagc ccagcgaaaa ggtcgtaggc
      841 gagttcgcag acgagatcga tgcggttgct gcagcaaggg ccgccaaggt tgatttcgcc
      901 atgtccgctt ccaaggccta cgcctggtgg gtggtccgcg aacaaggcgc acagctcgcc
      961 catttcatcg cagattccaa gagcgataag gaattcgtgc tcgatctgac ctctggccag
     1021 ctggtcgaag tctgattctc agccatttcc aattgaccgg ctaggaccct ttggcctgca
     1081 atcaaatcag gccggttaag cctcttccat caacaggtct gcgtactggt cgcgcacaac
     1141 cttcttatcg aacttgccgg tgccggtctt cgggatctga tcgatgaaga cgatccggtc
     1201 cggtatccac caggtggcca cccgctcctc gaggaaggtt tgaatcgccg cttcgtctgt
     1261 gctggccccc tcacgcaaga ccacgaatgc agccgggcgt tcctgccact tcacatgttt
     1321 gagcccgacc accgtggctt cggccacctc gggatgggcc atgatcgcgt tctcgagatc
     1381 gatcgatgag atccactccc cgcccgactt gatcacgtcc ttcgatcgat cagtaatcct
     1441 gatgtagccc tcaggggtga tcttggccac gtcacccgtg cgaagccacc cgtcctcgaa
     1501 ggtctgcgac gatctgggat ccttgtagta ctcggcggcg atccaagggc cgcggatcag
     1561 caactcgcca aaggcgacac cgtcctgggg caattcctcc ccgacctcat caacgatctt
     1621 gaccttgagc cccgcaatcg ggagcccacc ggtttccagg aagtcgagct ggcgttgcag
     1681 atcccagctc tccatctcgg gcttgagcac ggctgtagat gccaccgggt tggtctcggt
     1741 catcccccac ccctgcacca cccggatccc ccgttcggtc tggaaccact cgatcagtga
     1801 tcgcggaacc gccgagccgc cacagaggaa ggcccggatc gaggacacgt cggcctcggg
     1861 gtgcgcttcc aggtattgcc tgatccccag ccacaccgtg ggcacaccgg cgctgaacgt
     1921 cactctctcg ttctcgaaga ggcggaccag gccctcggca gacaggtcgg gaccgggata
     1981 ggtgagctgc gctccctggg tgaccgccat gaacgggtat ccccaggccg aggcgtggaa
     2041 cattggcacc accggaagca cgttgtcggt gggtcccata gggatattgg ccaggttgga
     2101 gatggtgtgc atgtaggtgg agcgctgggt gtaagccaca cctttgggat tgccggtcgt
     2161 tcctgaggtg taacacagca tcatcggaga ccgctcgtcg agctcgggcc atgggccatg
     2221 ttcgggtgcg tcggagatca ggtcctcgta ggcaatcgat cccggaatgc gctcgtcggc
     2281 ctccgggccc atcaccacat atgtcgccac cgtctcgagc ctcgggacca caggcgccag
     2341 aaggggaact agatccggat cgacgaaaag gacccggtca ccggcatggt tgacgatgta
     2401 ggcgacctgg tcggcaaaga gcctgatgtt gatcgtgtga cagatcagac cggtgttggc
     2461 cgtggcccag tagagctcgt gatgacggcg attgttccaa gcaaaagtgg ccacggcgtc
     2521 gcccattgag acgcccaacc cggcgagcgc cgatgcgagg cgacggaccc gagtgtcaat
     2581 ctccccgtag ttggtcctgg tcagcgagcg atcagcctcg accgaaagga tcgcctggtc
     2641 ggggtagagc cggacggacc ggtcgtagag ggtggacagg ttgagcggga aatcgtcctg
     2701 catgtggggc atcaggtgcc tcctatcaac ttttgcttct cggcctcgaa ctcctcatcg
     2761 gtcagcttgc cttcgtcatg gagtcgggac agcgtgtcca gctgactcgc cgtgttggct
     2821 ggcggtgccg ccgggacgcc accggtccgc atctgtgcca tccgcgcctc ccgtgcctca
     2881 tagatctgct tctgaacatg ctcgggatgg gggatgtcgg agtaggacga ctgcccatgg
     2941 gtcccggctg actcgatgag caggtcaccg gaaccgacga tccgctcgaa tgcggactgt
     3001 tggaaggtga catcattgac gacctcgagt gggatctcgg tccccttctt cgagatcaat
     3061 ccactgcgat gaatcactcg ctcattggtg atcacgtgct tgtcggtcca ccaacgcacg
     3121 aactcggcaa tcaccacgat gaaccacacc acgaccaagg cggttatcac ccaagctttg
     3181 gcgtcgaatc tccgggtggc aatcagaacg atcaggacca caaccaggat cgtgagcagg
     3241 ccctgcctga ggagccgaga ccaatgcggt cggaactcgg tcaagatctc ctcgcccgag
     3301 ctcagcagtt ttttcggata tcccatcgat gccttccgtt gctcagccga gaagctttcg
     3361 cttctcgttg tcgtactcgt cctgtgtcaa cttgccctcg tcgaacaact ttcccaggat
     3421 ctccaattgc tcagcgtttg acttgccggg cgatgcggcg gcgggtggag ccgccctccc
     3481 ggatgacatc gaattggttc ggtttcgccg agtttcgacg agcatcgatt tcaccttctc
     3541 aggctccggg atgttcgtga tccggttctg tcccgttgag cccgaggtct ggatcaccat
     3601 gtccccgaca ccaactgctc gatcgatcag gttctgacgg aagccgacgt cggtgatgac
     3661 ctccagggga atctcatacc ctgccttgcg catgatcccg gatcgaacaa ccaggcgctc
     3721 ggaagtgatc acgttctctc gggtacgcca ttccatgaac ccgccgaccg tgatccacag
     3781 ccagagcaag gtgatgatcg tgaaggccca tccgttgaca ccacctggca ccaggagaat
     3841 cagcagcacc acataggcga tggtgaccag ggcctccttc atgagcgcaa accaatggga
     3901 cccgaattcg tagacaaccg tttcgcctgg agcgaggaga tccttggggt acgccatcgc
     3961 cgggaggcta caggtcaggg gtgttgagtg cccccgtccc cggcttcgcc cgtactcccc
     4021 cgatgctcgc ttcgttcacc ggggagaaac gcgagaaggc cccgagggag catcggccga
     4081 agcctcaacc ctctcggggc tctcgttccg atcggcgtcg ggccggggac ccaactccgt
     4141 tcggagtcgt cgtaggccga agcctccgac gggcgctcac tggactgcct cgatccgaag
     4201 accgctgcga tccggtgacc gctctccccg tttccaggga ggtgcggatc cgaagatccg
     4261 ccggtcgaat ggccgcccga cgatcccgaa ggatccccgt tcggtctttc gaccgccccg
     4321 agcctcggtt tcccgatgct ccgggcaccc g
//
LOCUS       DBNHXU010000279         2663 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3465065, whole genome shotgun sequence.
ACCESSION   DBNHXU010000279 DBNHXU010000000
VERSION     DBNHXU010000279.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2663)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2663
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3465065"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..786
                     /locus_tag="AC161X_07025"
     CDS             <1..786
                     /locus_tag="AC161X_07025"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018786108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989338.1"
                     /translation="SVVPPTSGVTEVPSRGELELRGVGFSYPGASLAVLSDISFSARP
                     GQTTAIVGSTGAGKTTLVSLIPRLFDATEGSVLVDGVDVRDLDPDTLWSKVGLVPQKP
                     YLFSGTVASNLRYGKPDATEEEMWEALEIAQARDFVEEMPEGLESPISQGGTNVSGGQ
                     RQRLAIARALIRKPELYLFDDSFSALDLATDARLRRALKPTTADATVLIVAQRVSTIA
                     DADQILVLEDGEVVGLGSHNSLLATCPTYVEIVESQLRAEVAS"
     gene            783..>2663
                     /locus_tag="AC161X_07030"
     CDS             783..>2663
                     /locus_tag="AC161X_07030"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012895105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989339.1"
                     /translation="MSDRTMKTTERIQGQPGPGRGPFGGGMIAQKSASFGPSAKRLVG
                     RLRPQLPKVIAVFAAAIASVALSAVGPKVLGKATDLIFAGAIGKQLPAGMTKAEAIAA
                     AEAQGQTQFAKMLSSMDVVPGQGIDFHALGQVLLIVLALYAGSALLGYLQGFLLNDVV
                     QSTVYRLRSDVEEKLNRLPLRYFDRQPRGELLSRVTNDIDNVSQSLQQTMSQLLTSLL
                     TIIFVVAMMFWISPTLALIAMVTIPITMVVAGLVMKRSQGQFVTQWRRTGTLNAQIEE
                     AFTGHSLVKVFGRQKEVQATFEVENDGLYEASFKAQFLSGLIMPIMMFIGNLNYVIIA
                     VVGGLRVASGTMSLGDVQAFIQYSRQFTQPVTQVASMVNLLQSGVASAERVFELLDAD
                     EEVPDDSKPQMAAESHGRVVFEAVSFRYDEDRPLIEDLALSVEPGQTVAIVGPTGAGK
                     TTLVNLIMRFYELDRGRITLDGVDIAHLRRSDLRSQIGMVLQDTWLFHGTIRDNIAYG
                     RPDATEDEILEAARATFVDRFVHSLPDGYDTVIDDEGANVSSGEKQLITIARAFLADP
                     ALLILDEATSSVDTRTELLLQHAMAALRTDRTSFVIAHRLSTIRDADLILVMEEGRIV
                     EQG"
BASE COUNT          484 a          881 c          808 g          490 t
ORIGIN      
        1 tccgttgtgc ctccgacttc tggcgtcacc gaggtgccca gtcgaggcga gctcgagctg
       61 cgcggagtcg gtttcagcta ccccggcgct tcccttgctg tcttgagcga tatcagcttt
      121 tctgcccgcc ccggccagac gacggccatc gtcgggtcca ccggcgccgg caagacgacc
      181 ttggtgagcc tcatcccccg gctcttcgac gccacggaag ggagcgtcct cgttgatggt
      241 gtcgacgtcc gcgacctcga cccagacacc ctctggtcga aggtcggcct ggtcccccag
      301 aagccttatc tgttttcggg gaccgtggcc tcgaacttgc gatacggcaa gcccgacgcc
      361 accgaggagg agatgtggga ggccctcgaa atcgcccagg cgcgcgactt cgtggaagag
      421 atgccggagg gcctggagtc accgatctcc caggggggca ccaatgtctc cggcggccag
      481 cgacagaggc tcgcaatcgc acgggcgcta atccgcaaac cggagctcta cctcttcgac
      541 gactcgttct ctgcccttga cctggccact gatgcacgac tccgacgagc cctgaagccg
      601 accacggccg acgccaccgt cctcatcgtt gcccagcggg tgtccacgat cgcggacgct
      661 gaccagatcc tggtcctcga agacggggaa gtggtcgggc tcggcagtca taactccttg
      721 ctcgcgacct gccccacata tgtcgagatc gtcgagtcgc aactgagggc ggaggtggcc
      781 tcatgagcga tcgcaccatg aagaccacgg agcggatcca gggacagccc gggcccggcc
      841 gtggcccctt tggcggcggc atgatcgctc agaagtcggc gtccttcgga ccctcggcca
      901 agcgcctcgt gggccgactc cgtccccaac ttcccaaggt gattgccgtc tttgcggcgg
      961 cgatcgccag tgttgccctg tccgctgtgg ggcccaaggt cctgggcaag gccaccgacc
     1021 tcatcttcgc cggcgccatc ggaaagcaac tcccggcggg aatgaccaag gcggaagcca
     1081 tcgcggccgc cgaggcacag gggcaaaccc aattcgccaa gatgctctcg agcatggacg
     1141 tagtgcccgg ccaagggatt gacttccatg cgttgggaca ggttctcctg atcgtgctcg
     1201 ccctctacgc cggatcggcg ctgcttggct acctacaggg attcctcctc aacgatgtgg
     1261 tccaaagcac cgtgtatcgc ctgcgctccg acgtcgagga gaagctgaat cggctcccgc
     1321 tgcgctactt cgaccggcag ccccgtggcg agctgctgag tcgggtgacc aacgacatcg
     1381 acaacgtgtc acagagcttg cagcagacga tgagccaact cctcacttcg ctgctcacca
     1441 tcattttcgt ggtcgcgatg atgttctgga tctcaccgac ccttgccttg attgccatgg
     1501 tgacgattcc catcaccatg gttgtcgccg ggctcgtgat gaagcggtcg cagggtcaat
     1561 tcgtcactca gtggcgtcga accggaaccc tcaacgctca gatcgaggag gccttcaccg
     1621 ggcattcatt ggtcaaggtc ttcggacggc agaaggaggt tcaagccacc ttcgaggtcg
     1681 agaacgacgg gctgtacgag gccagcttca aggcccaatt cctctctgga ctgattatgc
     1741 cgatcatgat gttcatcggg aacctgaact acgtgatcat cgccgtagtg ggcgggcttc
     1801 gggtggcgag cgggaccatg agccttggtg acgtgcaggc cttcatccag tactcccgcc
     1861 aattcaccca gcccgtgacc caggtcgcgt cgatggtcaa cctcctgcag tcaggagtgg
     1921 cctctgcgga gcgggtgttc gagctcctcg atgccgatga agaagtgccc gacgacagca
     1981 agccgcagat ggctgcggaa agtcacggcc gcgtcgtctt cgaagccgtg tccttccgct
     2041 acgacgagga ccgccccctc atcgaggacc tcgccctctc ggtggagccg ggacaaacgg
     2101 tcgccatcgt ggggccaacc ggggccggaa agacgactct ggtgaatctg atcatgcggt
     2161 tctacgagct tgaccggggt cgaatcaccc tggacggagt cgacatcgcc catctccgtc
     2221 gctccgatct tcgctctcag atcggcatgg tcctccagga cacctggctg ttccacggga
     2281 cgattcggga caacatcgcc tacgggcggc ccgatgcgac cgaggacgag atactcgaag
     2341 cggcccgggc cacctttgtc gaccggttcg tgcacagcct gcccgacggt tacgacaccg
     2401 tcatcgatga cgagggagcc aacgtcagca gtggtgagaa gcagctgatc acaatcgcac
     2461 gggcgttcct tgccgatccc gccctactga ttctcgacga ggcgaccagc tcggtcgaca
     2521 cccgcaccga gctcctcctc caacatgcga tggcggcgct tcgaaccgac cgcaccagct
     2581 ttgtcatcgc ccatcgcctg tccacgatcc gggacgccga tctgatcctg gtcatggagg
     2641 agggccggat tgtcgagcag gga
//
LOCUS       DBNHXU010000280        23528 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2474036, whole genome shotgun sequence.
ACCESSION   DBNHXU010000280 DBNHXU010000000
VERSION     DBNHXU010000280.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 23528)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 23528)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23528
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2474036"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..550)
                     /locus_tag="AC161X_07035"
     CDS             complement(35..550)
                     /locus_tag="AC161X_07035"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4989340.1"
                     /translation="MDELTTALAVDLDRAFPDLVKDLGPRLYWGLRRMCGDHQEAEDL
                     TQETFIRAYKALGDFDRQRFDDLRLAPWVWTIALNLGRNHIRDRSRRPLFVEMNDHGV
                     EDAELPDERAWDSRFAALNRNQRTAVVLRHVVGLGIGEISETTGRPEGTVKADIHRGL
                     DRLREMMEHEA"
     gene            615..1289
                     /locus_tag="AC161X_07040"
     CDS             615..1289
                     /locus_tag="AC161X_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989341.1"
                     /translation="MDDSEITETLRLAEVAVKAGDPLGPTGFWKAVAAVKANPTLIEA
                     NADRIGVIDSIAFRNWALLTVPLWIGNLLTLGGTLLGLALIAWAYSLDGTSAGIAFLF
                     GFGALLVTTHSLGHLVVGAFLGIRFTRWFVGSIRRPQPGVKVDYASYLRVPAKSRAWM
                     HASGAIVTKIVPFALIGAALAAGVPSWVVWVLVVVGIGQLVTDVFWSTKASDWKKYKR
                     EMAFTR"
     gene            complement(1286..2938)
                     /locus_tag="AC161X_07045"
     CDS             complement(1286..2938)
                     /locus_tag="AC161X_07045"
                     /inference="COORDINATES: protein motif:HMM:NF017760.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4989342.1"
                     /translation="MTSTVAPLRIESFRTLWIASLVSNVGSLIQTVAANWLMFEMTHS
                     NTWVGLMVASSTLPLLFLALAAGALADMFDRTKLMLISQVIMGGSALAMAILTATDLI
                     TPGWLLGLGLILGTGVALNLPTWNALLPDLVPRLMIPNAVALNSAGFNVARAIGPAIG
                     GLIVATLGAQAGFAINALSYAAVVVALLMIGRSINSPIRESTSFGTAISLGVRYARYT
                     PVFRRLLLLVALFAITSAVVQSVLPGRTEALGGEAGALGALLAAMGAGALLGAFVRQR
                     FVGRLGRRAPMATITLFGVAGIGVGLAPNLAWAFVAMFFSGAFWVLTLTNLNATAQLM
                     APHWIRGRAMSFYTLAFGGVLPLGAILAGIVADAIGTGQAMVVLSLGTVALGLASPAF
                     KLPTLDEVESPEFNEERIPPVHEEIAEGGPVMILNTWTIDEGDFAEFTDLMNEIRLVR
                     LRTGAYQWSLFRNASTPTRLTEMFATGSWEEHLAQHRRIDDASAALIAKARAFDRADG
                     PVTRHLIAIDVEDPPNFDELLLTHEQMHQTDGSIPTMVEQEN"
     gene            2972..3607
                     /locus_tag="AC161X_07050"
     CDS             2972..3607
                     /locus_tag="AC161X_07050"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013866.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HIR4989343.1"
                     /translation="MVRDLAYFIQGDGGPGPGVLLLHSFWGLTSSVKGLADDLADHGF
                     TVLAPDINFGELPESEQEGLDHLGKADPNRLASLVLSSAKLLHERSTEGPIGVVGFGM
                     GGSLGLWASARLVDVVGAVVSFYGSQQIDFAGATAAYLVHLAADDEFITDDEAAFMEA
                     TMRLEELEVEVIQHPGTKHGFGEPDGDTFDPEAFDAAWRQTIEFLEARLNL"
     gene            complement(3604..4161)
                     /locus_tag="AC161X_07055"
     CDS             complement(3604..4161)
                     /locus_tag="AC161X_07055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020602354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HIR4989344.1"
                     /translation="MPHIQLPPELPGISSAFAFSPETAKPMRELAEVLLRGPNTLSSG
                     EREMIATFVSSRNDCGFCQDSHRAAAAHHLDGDYELVDAVKNDYQSAPISPKLKALLA
                     IAGKVAESGRNVTSADVDQARDEGATDKEIHDTVLIAAAFSMFNRYVDGLETWTPADP
                     EMYDVMGKRMAHEGYVRSDRATQAG"
     gene            4198..5778
                     /locus_tag="AC161X_07060"
     CDS             4198..5778
                     /locus_tag="AC161X_07060"
                     /EC_number="1.2.1.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009376823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinic semialdehyde dehydrogenase"
                     /protein_id="HIR4989345.1"
                     /translation="MAVRDQQLTPRVGAALLRDLESRVDVPVANHELIDVDSPFTLEV
                     IGQIPQATAADVDAAVAIAREAQPAWAAMGVKRRAAILLSFHDRLMKEVDTVVDIIQL
                     EGGKTRAGAWEEVVDVAGTARYYANTAPRLTRRKRRQGAMPLFTKTHEYRHPKGVCGF
                     ISPWNFPFNLSIGDAIAALVAGNAVVIKPDERTPFSLLAAVRLFDEAGAPDGVIQVVT
                     GRGEICGPALIDAVDYVMFTGSSAVGSLIAEQAGRNLIGSSMELGGKNAAIVLDDADL
                     DRTIPELARGTFGHAGQMCVHTERIYVERGIEDEFTRRFVEQAERLEISAGFEFGPRV
                     GSLITQDHLAEVEAHVEDAVAKGANLLTGGQRRPDVGPLFYAPTIVTNVDETMNLCRG
                     ETFGPVVSIYPVSSADEAVKLANDSNLGLSFSVWSRDTRRAEAIAVRLEAGSVAVNDG
                     YVATWGSHDAPMGGFKRSGLGRRHGWEGLLKFTEPQTVAVQRLIPAYAPALGLSYETY
                     RKLVERLVMVFRRLPFYK"
     gene            complement(5787..6266)
                     /locus_tag="AC161X_07065"
     CDS             complement(5787..6266)
                     /locus_tag="AC161X_07065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989346.1"
                     /translation="MTEPVKDPTPEQSAAWHRTFAPQAFNRTWELLDLDVRTPEEDEE
                     MLASTFTQRFHWYQIGDPRNRAIADWQVSRVAAVLGYAELSQRFGERSLAVCEEDDLD
                     AFVRGFAHEAIARAAAEVDDVETFTEHLELAMALLPDIEDTEDRDILAADLAEMSEE"
     gene            6314..7315
                     /locus_tag="AC161X_07070"
     CDS             6314..7315
                     /locus_tag="AC161X_07070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015612648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HIR4989347.1"
                     /translation="MSDHTTVIVGGEEVRISSPDRIIFPRRGWSKLDVVEHFVVVAEG
                     AIRGIAGRPTMLKRYMGTVDEDPIYHKRVSNNSPFDSVQIRFPSQRPGRMNVPRTQAD
                     VVRLAQLGCLDFHPWPVRAEDTDHPDELRLDFDPGPGVTFEQVRAAAQAARTLLDEIG
                     LVGWPKTSGSRGIHVYVRIEPLWDFFQTRRAVLAFARELERRMPGEVTTKWWKEERQG
                     VFIDYNQNARDKSVSSAYGVRPTGFVSAPITWDELANVAIEDFPLDTFRDRYSKVGDL
                     SDGIDEAAGRIDTLLEWMARDEENGIGDAPWPPHYPKMPGEPPRVQPSRQRRPDEDY"
     gene            7364..7684
                     /locus_tag="AC161X_07075"
     CDS             7364..7684
                     /locus_tag="AC161X_07075"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR4989348.1"
                     /translation="MPRSQMVKGLVDLGVLTVLNHGESYGYEIMTWFEKAGLDDVGHA
                     SVYGSLKRLEEQGCVATEERPSPLGPARRYYRITPRGKEYRAGLHKAWSGLQSALEIL
                     ERGM"
     gene            7681..8808
                     /locus_tag="AC161X_07080"
     CDS             7681..8808
                     /locus_tag="AC161X_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989349.1"
                     /translation="MTPSEQYAREVVSHLERLPRAKRSRLRRELAAELAEYPEAEDRE
                     ALEARLGTPSRYAADLAEELGVRPGRRSRPRGWGWGWVAAVLVVVGWLLLANLTTPLE
                     PGSFSSSGSFEVTVGGDPFDEWTEVVYHQDQEGEMSSSLRNRARLSVTITDIGVFPAM
                     DRSSRELTGWSGLFVQTGEFYEGDTPPGYGFMDDPVSFTESPLVLAPGEERTIAIQAM
                     LTNCEFNTPGASEGHDAAMVTYSYLGLTKVATVQFPKVFTTSPQVCEAPVNREFATAP
                     EAIADGIAPSWIPSDARDVLLATGEAGSVLSLTTDQLAPDTCKGSNASPYWSYRPSWA
                     PSQDLVAAKDVLACPEDWFWWGPEPGEVLMWTETEYPFVAP"
     gene            complement(8805..9533)
                     /locus_tag="AC161X_07085"
     CDS             complement(8805..9533)
                     /locus_tag="AC161X_07085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989350.1"
                     /translation="MTLALHLYRRMMRRGRVIALVALASVSGLVYWLVGFDAPANDLT
                     GLYTDILASVGYTFVVAALILTAATLREEKDAGTLPYIYLRPIPRAWFAGQALIAGSA
                     AAIVIGICGWLVTVVASIAVGNGIGPTIPALTLFVAAGIGYAAVFVPLGYLAPRSLLI
                     GLGYIIVWETILANVVTGLAQFSIWRISLSIYAGMEDEFGQVATDTLGPVTEGVGGGV
                     AKIAVVVVVGLVVLTWALRRRDAL"
     gene            complement(9533..10450)
                     /locus_tag="AC161X_07090"
     CDS             complement(9533..10450)
                     /locus_tag="AC161X_07090"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989351.1"
                     /translation="MTDTIVVTELSKWFGLKVAVSELTIGFRPGVTGLLGPNGAGKTT
                     LLRVMTGLQRPSQGSIQVLGLDPRDHPSIYNRIALVPEDETVYDRLTGRQFVELAARL
                     AKVDRVKERVSAAIEDVGLTDAADRALGGFSKGMKQRAKVAQALVSDPEVLLLDEPLN
                     GADPVQRAHLIDLFRKLGDQGKTVIVSSHVLAEVERMTDRVVAMVDGRLAAVGDVSTI
                     RAAMTDKPRRVYVEASDPRRLASSLIASPGVVGVSIDGDTVHVEASDSGDLARRLPIL
                     AAEGGIRLTRVEPVDESLESVFRYLVEGR"
     gene            complement(10447..11328)
                     /locus_tag="AC161X_07095"
     CDS             complement(10447..11328)
                     /locus_tag="AC161X_07095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989352.1"
                     /translation="MTDQVIFDRGYRTYDGPRLGPAGARRAVFKDGVRRVLGLGRKAR
                     EKIFPWVLIAIAVIAAAVFLGINWAAGNIDEAIQEGAPTYGDLFDFYSAISILFIAFA
                     GPKLLIPDRTKGVLSVYFSRPLTVDGYLTSKVGAFATVVGAIYIVPQLILHLGLSLLS
                     DDGFLTYLGDNLDVLWKVPVTTLAFVALHGSLIFILASVIDRTGIAAAAFLGILVAGD
                     GIAGRVAEATFPGAKFASLVAPGQHPRIIRDYFFGDTIAYPAEIAGFDVWVSVAVVVG
                     VTVAAVWFIRWRYRKLA"
     gene            complement(11325..12227)
                     /locus_tag="AC161X_07100"
     CDS             complement(11325..12227)
                     /locus_tag="AC161X_07100"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989353.1"
                     /translation="MTVLEVSDLTHDYGEVVAIRNLTLEVPEGVTGLVGANGAGKTTL
                     LRLMLGLLHPTSGSMQVLGHDPQRDPLAVRTVIGYMPEGDCLPKDQTAADFVAYTAQL
                     AGIPPREARRRASETLFLVGLEEERFRFLGDFSTGMQQRVKLAQAIVHDPDLLLLDEP
                     ASGLDPEGRTQMLELIRRLADFKVNVILSSHVLTDIEQTCDWVVMLDAGDLLRSGPLE
                     GFEKLGTVVVEVIDHADKVAEALRRRGFEATVEGQRLLVGPGDEKLEEAIVAETAAAN
                     SGLVRMIRGSASLEDLFLAREASQ"
     gene            complement(12224..13246)
                     /locus_tag="AC161X_07105"
     CDS             complement(12224..13246)
                     /locus_tag="AC161X_07105"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005623616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR4989354.1"
                     /translation="MSHPFPAPIETMEAKVSEGWPKGTFLYEPKWDGFRSVSWSQPDI
                     RLDSRSQRPLLRYFPELRPALEQLPDGTVIDGEVIVVVDGATDFGTLQQRIHPAESRI
                     KMLSEATPAELVAFDLLAHQGEDLRAAPFVERRERLMSVAADLGHPWHLTPQTESEET
                     ATRWFSEFESAGCDGIIAKLPDLAYQHGKRAMIKIKHRRTVDCVVGGFREHKDGGKIG
                     SLLLGVYDGQGQFHFIGHCSGFPDGQRVEIYNRFLELVAADSFDEDARAPGGQSRWSS
                     AKDGAWTPVQPGVVVEVSYDQLEGNRFRHATRFHRWRPEKDPLSCTMEQLERPEGPGF
                     AEVVAG"
     gene            13260..14276
                     /locus_tag="AC161X_07110"
     CDS             13260..14276
                     /locus_tag="AC161X_07110"
                     /inference="COORDINATES: protein motif:HMM:NF015587.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HIR4989355.1"
                     /translation="MRRYAGLIIERIVERLPKWAKGPAEIVATSAKEYSIDRAPRMAA
                     AIAYRTVFALAPMLIIAVSVAGAFLGNAEVQREVLEAVERIAGPQVATTVATILATAV
                     SSTNTAAIIGVVLLLWSASSLFIELQHDLNDIFDVPYERISGLIGLVRKRGIGFLWVF
                     GLGLVMLALLVINAIWGFLDSLLPESLENLDTVFQVLGPIVSLAILPLVFALIFQTLT
                     VVPIPWRAVWFGALFTAVVFTVAAYGIGIWFQIFGVTTALGFAGSLVVVLFMAYMLSS
                     VFLFGAEVTRAYADRLAAARVKAPQRPVDPLVVVSEPPRAIPGAAVVAFLTGLVIGWR
                     RRDR"
     gene            14324..14890
                     /locus_tag="AC161X_07115"
     CDS             14324..14890
                     /locus_tag="AC161X_07115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989356.1"
                     /translation="MSIDIAGFVTDLKEHATEHGFHVHDERHFVETYSLRQSWEVDLH
                     PEAGCGGPIDLHLALDGDPRVILSLRERLEEMDDDFEEPEDVYHLDLFFNWAVPPLHN
                     PPDLLVLATDLAGIGRLSLVVEVQAIDSYGAVTDAPERRLTLTGKSSVSLVDVLMGRE
                     QLCDVLDRSHDVSVYLLDRVAGWMELPT"
     gene            complement(14891..15307)
                     /locus_tag="AC161X_07120"
     CDS             complement(14891..15307)
                     /locus_tag="AC161X_07120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989357.1"
                     /translation="MTTFPGTVTFEDDRVPVMVGIDIDKITLISGEVEIGEWPTAECT
                     FVEKGDGSWVIEAEDDSVFFLPDDPSRFAQGLAGHLIIDADALTETSAAHSEADNGVF
                     DVAEGPSPKVLTVVGFYALCAITLALGVWAAVSLIG"
     gene            complement(15369..16064)
                     /locus_tag="AC161X_07125"
     CDS             complement(15369..16064)
                     /locus_tag="AC161X_07125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2461 domain-containing protein"
                     /protein_id="HIR4989358.1"
                     /translation="MAKQYFSSEVFDFLTELSRNNNKIWWDHNKERYIEVVREPALDF
                     IQDFGPRLTRISPHFTADARTIGGSLMRPYRDTRFSKDKTPYKTNVGIQFRHDAGKDI
                     HAPGFYVHLEPDACFAGVGMWHPETPVARRIRQAINDDPSGWKQATKGKAFTNTWSIE
                     QDEDETLKKVPREFDQDHPYADDLRMKSFVAGTRMSDQFVASAGFDTRLIAMFSKASD
                     FTAFVCKAIGLPF"
     gene            complement(16126..17448)
                     /locus_tag="AC161X_07130"
     CDS             complement(16126..17448)
                     /locus_tag="AC161X_07130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton exchanger"
                     /protein_id="HIR4989359.1"
                     /translation="MESVDLNPRRQILYIGLAVAATLPGVFLRLTGTHPAPVVGAFLF
                     GLAIVGSAFILSWAAEVIQLDIAAGLALALLALIAILPEYAVDFVFTAKAGQEFAETG
                     AATQYGPLALANMTGGNQLLIGFGWPLVILLGTYRVRKTKASPERAEGTTATTINLDR
                     SQSVEIAYLTIASLYGLTLFLKDTLTLWDAGILVFIYVLYLRRLSGAPPSTPHLVGPS
                     AYIGSLPTRLRRFINYIGFVVAAGVILLCAEPFAEAIVEMGQTLGVSEFLLVKWIAPL
                     ASEAPELLVAALFAWRLAARTGFGALISSKVNQWTLLVGTLPIVFSIFSRTTHGLPLD
                     RQQRVELMVTAAQSVFAVAIVASRSVTTKEAWAMFALFVAQLLESGLAELGHISEEGS
                     AQARIGVGIVFLLAAAWVLRKDFRKLVVVVREGLRTPWHELAKEDTPV"
     gene            complement(17485..18648)
                     /locus_tag="AC161X_07135"
     CDS             complement(17485..18648)
                     /locus_tag="AC161X_07135"
                     /inference="COORDINATES: protein motif:HMM:NF013618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HIR4989360.1"
                     /translation="MRCRRTLSSLLAAVLAAAVLISTADGARAQSVGDAENAAEEAQQ
                     QAGAASGLVDEALANRSDIESQLAASMVRLDELAEQLSSVAAGVDRLQGQIGFADAEL
                     LGIKADIETQAVDAYMSALSSPGVSLANSDNVEDALVAGLFIGDVVATGQENVNALVM
                     KRRDLEELVATFLADQERVAELQAEVDAETENLASLYEQADASVAEAIREASKSAAEY
                     SEALNAVDAARAREAEQERQDERDPPTTTTPDTPNSTNAPTTTTTVAVTTTTDDDSGG
                     GGGSTNFPAAVERWRDEVSRYFPASRVNEALAIIQCESLGDPEAYNPYSGASGLFQFL
                     PSTWAATASKAGFPNSGPFDSLANIGTAAWLGQRYQDLGQGFWKPWSCRRVLN"
     gene            18792..19136
                     /locus_tag="AC161X_07140"
     CDS             18792..19136
                     /locus_tag="AC161X_07140"
                     /inference="COORDINATES: protein motif:HMM:NF013228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HIR4989361.1"
                     /translation="MGFSRAVRVGDRVLVAGTAPIWPDGEVDPDPATQARRCLEIILD
                     ALYQAGARASDVVRTRSFLTDASYADAVGSAHGEVFGQVRPASTMVVVAGLLDERWKV
                     EIEAEAIVSSSV"
     gene            complement(19143..20045)
                     /gene="ypfJ"
                     /locus_tag="AC161X_07145"
     CDS             complement(19143..20045)
                     /gene="ypfJ"
                     /locus_tag="AC161X_07145"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF050010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KPN_02809 family neutral zinc metallopeptidase"
                     /protein_id="HIR4989362.1"
                     /translation="MVRYKKEPVSKGLEDRRGQTTSRRGIALGGGAGGIGLIVVLLVM
                     FLGGGGIDPGALDDITDALAPGVVAPENEGTIAPTADPDADLKDYMNAVYTDLDLMWT
                     DVFIAAGRDDYRMPGFVIFEDYTDSGCGGADETVGPHYCPVDEKIYLDFDFFTLLEDQ
                     FGAGGDFAPAYVIAHEFGHHIQTVIGVSDEVRALQQQNPDQANELSILMELQADCFAG
                     VWASTLAVGTGTDESVELDPGEIQEAIEAAEAVGDDRIQSQVTGQINPETWTHGSAEQ
                     RAQWTMTGYDSGDPGACDTFSAGG"
     gene            20123..20758
                     /gene="msrA"
                     /locus_tag="AC161X_07150"
     CDS             20123..20758
                     /gene="msrA"
                     /locus_tag="AC161X_07150"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007616082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 - L-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HIR4989363.1"
                     /translation="MFGRKKSEMTGASDALPGRDRSVVVPPTHFVNGNPLTAPFPADT
                     EVAVFGMGCFWGAERRFWKMDGVYSTAAGYAGGYTPNPSYEEVCTGRTGHSEVTLVVY
                     RPAEVSYEELLRTFWETHDPTQGMQQGNDIGTQYRSAIYTTTDEQLEIAKRSQDKYQA
                     ELTPLGFGDITTEIAPLGEFYYAEDYHQQYLAKNPHGYDCHANTGIAYPIA"
     gene            complement(20765..22141)
                     /locus_tag="AC161X_07155"
     CDS             complement(20765..22141)
                     /locus_tag="AC161X_07155"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR4989364.1"
                     /translation="MRLVALALVVTIAACTEVAGNEGTQPVSTSSTTPTASPEPPRRD
                     LETVTCDQATEDVEIVCEAYELVRRHYVDDPADVDLAGAAALGVADLDGSTSTSALVC
                     AEPTDEFDATCRSIVDEAETTSEAAEAMVAGLATYGLDANSVYLDRESLALLEEEQDG
                     EIHGIGALVIAEDSSTGTKAQCQVVSATCHLLIVSTIEGSPAQSAGLMRDDVILRVDG
                     ADVDGWTVDEVTSTVRGQAGSEVTLTVLRDGDQFDVTIIRADVVIPVVESQLVGHTGY
                     VRLNLFTGDADEQFEDAVTTLLTADADSLVIDLRDNPGGLLDTAIAVTSVFLADGDVV
                     VTEGPEGSTSYEVSGHPLVPSDLDVVFVVNKGSASASEVVSAVLQERGRATVVGENTF
                     GKNTVQQRFGLSNGGALKLTVARWVTPGGLDFGETGVSPDVEQRIDGGLAVDSVVRMA
                     LEASLRAS"
     gene            complement(22150..>23528)
                     /locus_tag="AC161X_07160"
     CDS             complement(22150..>23528)
                     /locus_tag="AC161X_07160"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF015531.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HIR4989365.1"
                     /translation="KSVLVAALLVSACNSGAGQATTTTAATPTTTALRPTTTTPSLPV
                     QVDDCSAPQVSFSALCETYQLIQEWHVDRPLDATALAAAALEGLDNFATDQTADPPRA
                     LMCAIPDAAFGDLCEHLADRVDSESIPIGPAVEAAVSEMANHGLDQFSYYVPPDQVGA
                     FRSNGVVGGVGALLDATDAVGSHCARVTASCPLRVVFVVEDNPAEAAGLLAGDVITAI
                     DGTSIDGMGFVDVVSQIAGDETGTVAITVQRVDGQSDVLDIERDELTIPTVEIDAPRP
                     RVGLIRIPDFEDDIPDLVHLGLTDLIDYGIDTLIVDLRDNPGGYVDAAVLVISEFVED
                     GVIFHTSDPEAENDVTAVGGGLATGVDLIVLVNQGTASAAEITATALRDRRGAVIVGE
                     PTFGKNAVQIPFELRNGGELYVAVAHWTSPNGDSVAETGIAPDHLVSLPAGLTKEELV
                     DLSVEASG"
BASE COUNT         4290 a         7291 c         7595 g         4352 t
ORIGIN      
        1 ggtccagcga ggccctcgag ttgttgttcg atattcatgc ttcgtgctcc atcatctctc
       61 gcaatcggtc caaccctcga tggatgtctg ccttcaccgt gccttcgggg cggccggtcg
      121 tctccgaaat ctccccgatt ccgagcccga caacgtgacg caagacgacg gccgtgcgtt
      181 ggttgcggtt gagagccgca aatcgagaat cccaggctcg ctcatccggt agctcggcgt
      241 cctccacccc gtggtcgttc atctccacga atagcggccg ccgcgatcgg tcccggatgt
      301 ggtttcgacc aaggttgagg gcaatggtcc acacccacgg ggccaatcga aggtcatcga
      361 accgctgtcg gtcgaagtct cccagggcct tgtaggccct gatgaacgtc tcctgggtca
      421 gatcctcggc ctcctggtgg tccccacaca tgcggcgcag tccccaatag agccggggac
      481 caagatcctt gaccagatcc gggaaggctc ggtcgaggtc cacggcgaga gcggtcgtca
      541 gctcatccat cgtcatcaga acacccggga cagtgattcg gttgcagcgg tcgcaccgta
      601 cttagataag ggcagtggac gactcggaga tcaccgagac cttgcggctt gccgaagtgg
      661 cggtcaaggc tggtgatccg ctcggcccga ctggtttctg gaaagcagtg gcggccgtga
      721 aggccaaccc gacattgatc gaggccaacg ccgaccggat tggggtcatc gattcgatcg
      781 cattccgaaa ttgggcgcta ttgaccgttc ccctctggat cgggaacctc ttgaccttgg
      841 gaggcaccct tctcggcctt gcgctcatcg cctgggccta ttcgctcgac gggacctccg
      901 ctgggatcgc ctttctgttt ggtttcgggg cgctcctggt cactacccac agtctcggcc
      961 acctcgtggt cggagccttc ctggggattc gcttcactcg atggtttgtt ggctcgattc
     1021 gccgccccca gccgggcgtc aaggtcgact acgccagcta cttgcgagtc ccggcgaagt
     1081 cgcgggcctg gatgcacgcc tcgggggcga ttgtcaccaa gatcgttccg ttcgcgctga
     1141 tcggcgccgc cctggccgct ggagtgcctt cgtgggtggt gtgggtgctg gttgtggtcg
     1201 gcattggcca gctggtcaca gatgttttct ggtctacgaa ggcgtcggac tggaagaagt
     1261 acaagcgcga gatggctttc acccgctagt tctcttgctc caccatggtt gggatcgatc
     1321 catcggtttg gtgcatctgc tcgtgggtga gcagcagttc gtcgaagttg ggcgggtctt
     1381 cgacatcgat cgcgatcagg tgccgggtga ccggtccgtc ggcccggtcg aaggcccggg
     1441 ccttggcgat cagggcggcc gaggcgtcgt cgatgcgccg gtgctgtgcg agatgctcct
     1501 cccaagaccc ggtggcgaac atctcggtga gccgggtcgg ggtcgaggcg ttccggaaca
     1561 ggctccattg gtaagccccg gttctgagac gaaccagacg gatttcattc atcaaatcgg
     1621 tgaactcggc gaagtcgccc tcatcgatgg tccaggtgtt gagaatcatc accggaccac
     1681 cctccgcgat ctcttcgtga actggcggga tccgctcctc gttgaactcg ggcgactcca
     1741 cctcatcgag ggtgggcagc ttgaacgcag gcgatgccag gcccaaggcg accgttccca
     1801 gggagaggac gaccatcgcc tgcccggtgc cgatggcgtc ggcgacgatc cctgccagga
     1861 tcgcgcccag ggggaggacc cccccaaacg ccaaggtgta gaacgacatt gcccggcctc
     1921 gaatccagtg cggcgccatg agttgggcgg tggcgttgag attggtgagg gtgagcaccc
     1981 agaacgcccc ggagaagaac atcgccacga atgcccaggc gaggttgggc gccaaaccga
     2041 ccccgattcc cgcgacgccg aaaagggtga tcgtcgccat cggagccctt ctccccaaac
     2101 gtccgacaaa ccgctggcgg acaaaggctc ccaacaaggc accggcaccc attgccgcca
     2161 gcaatgcgcc gagagcccca gcttcaccgc ccagtgcctc ggttcgtccc ggcaggaccg
     2221 actgcaccac ggccgaggtg atggcgaaca gggccaccag cagcaagaga cgtcggaaca
     2281 ccggcgtgta gcgggcatag cggactccaa ggctgatcgc ggtcccgaac gaggtgctct
     2341 cccggatcgg actgttgatc gagcggccga tcatgagcag agccacgacc actgcggcgt
     2401 agctgagggc attgatcgcg aagccggctt gggcgccgag ggtggcgaca atgagcccgc
     2461 cgatggcggg cccgatggcc cgggccacgt tgaaacccgc cgaattgagc gccaccgcgt
     2521 tgggaatcat cagccggggc acgaggtcgg ggagcagggc gttccaggtg ggcaggttga
     2581 gcgccacccc ggtgcccagg atcagcccga ggccaagcag ccagcccggt gtgatcaggt
     2641 cggtggcggt gagtatcgcc atggcgagcg ccgagccgcc catgatgacc tgtgagatca
     2701 gcatcaactt ggttcggtcg aacatgtcgg ccagggcccc cgccgccagg gcgagaaaga
     2761 gaaggggcag ggtgctcgag gcgaccatca gccccaccca ggtgttggag tgggtcatct
     2821 cgaacatgag ccagttggcg gcgacggtct ggatcaggct gcccacgttc gagacgaggg
     2881 atgcgatcca gagcgtgcgg aacgactcga tccgaagtgg ggcgaccgtc gaggtcatgg
     2941 ccacaagcgt aggagcggta ggttccaccc agtggttcgc gatctcgcat acttcatcca
     3001 gggggacggg ggacccggtc cgggagtctt gctgctccac tcgttctggg gtctcacatc
     3061 atcggtgaag ggactggcag atgatctggc cgaccacggg ttcactgtcc ttgccccgga
     3121 catcaacttc ggcgagctcc cggagtcgga gcaagaaggc ttggatcatc tcggcaaggc
     3181 cgatcccaac cggctcgcct cgttggtcct ttcatccgca aagctcctcc atgaaaggtc
     3241 gacagagggc ccgatcggag tcgtcggttt tgggatgggt gggtcgctcg gattgtgggc
     3301 ctcggctcgt ctcgtcgatg ttgttggggc tgtggtctcc ttctatggga gccagcagat
     3361 cgacttcgcc ggcgccaccg ccgcctatct ggttcacctc gccgccgatg acgagttcat
     3421 cacggatgac gaagccgcat tcatggaggc caccatgaga ctggaggagt tggaggtgga
     3481 ggtgatccaa cacccgggca cgaaacacgg tttcggcgag ccggatggcg acaccttcga
     3541 tcccgaggcg ttcgatgccg cctggcgtca aacgatcgag ttcctcgagg ccaggctgaa
     3601 tctctagccg gcttgagtcg cccggtccga gcggacatag ccttcgtggg ccattcgctt
     3661 ccccatcacg tcatacatct cagggtcggc gggtgtccaa gtctcgaggc cgtccacgta
     3721 gcggttgaac atggagaagg cggctgcgat cagaacggtg tcgtggatct ccttgtccgt
     3781 ggctccttca tcccgcgcct gatcgacatc tgcgctggtc acatttcgac cgctctccgc
     3841 aaccttgccc gcgatcgcta ggagcgcctt gagcttcgga gaaattggtg cgctctgata
     3901 gtcgttcttg acggcatcca cgagctcata gtcaccgtcg agatggtgag ccgccgccgc
     3961 tcgatgactg tcctggcaga aaccgcagtc atttcgggat gagacgaagg tggcgatcat
     4021 ctcgcgctcc ccgctgctca gcgtgttcgg ccctcgaagc agcacctcgg ccagttcccg
     4081 catgggtttt gcggtctccg ggctgaatgc aaaggcgctg ctgattccag gcaattccgg
     4141 tggtagttgg atatgtggca cgcggccgat tgtaaggaga gcgggtaccg ttagaaaatg
     4201 gcggttcgcg accagcaact gacgccacgg gtcggggcgg cgctgctgcg ggacctggaa
     4261 tcccgggtcg atgtgccggt ggcgaatcac gagctgatcg acgtcgactc cccattcacc
     4321 ctcgaagtca tcggtcagat cccgcaggca accgccgctg atgtcgatgc ggcagtggcc
     4381 atcgcacgag aggctcagcc tgcctgggcc gcgatggggg tgaagcggcg agcagccatc
     4441 cttctctcct tccacgaccg tctgatgaag gaagtcgaca cggtggtgga catcatccag
     4501 ctcgaggggg gcaagacgag ggccggggca tgggaggagg tggtggatgt cgccggaacg
     4561 gctcgctact acgccaacac ggcgccgcgg ctgacaagga gaaagcgccg ccagggggcg
     4621 atgccgctct tcaccaagac tcacgaatac cgtcacccaa agggagtttg tggcttcatc
     4681 tccccctgga actttccctt caatctctcg atcggcgatg ccatcgcggc gctggtggcc
     4741 ggaaatgccg ttgtgatcaa gccagacgag agaacgcctt tctccctgct tgcagcggtc
     4801 cggctcttcg acgaggccgg tgctcccgac ggcgtgatcc aggtagtgac gggacgcggc
     4861 gagatctgcg gccccgcgct catcgatgcg gtcgactatg tgatgtttac cggatcgagc
     4921 gcggtgggga gcctcatcgc cgaacaggcg gggcggaact tgatcggctc gtcgatggaa
     4981 ttgggcggga agaatgccgc catcgttctc gatgatgccg atctggatcg gacgattccc
     5041 gagctggcga ggggcacctt cggccatgca ggtcagatgt gcgtccacac cgagaggatc
     5101 tatgtggaac gggggatcga agacgagttc acccggcgat tcgtcgagca ggcggagcgt
     5161 cttgagatct cggccggctt cgagttcggc cctcgggtcg gctcgctgat cacccaggac
     5221 catttggccg aggtcgaggc gcatgtcgaa gacgcggtgg ccaagggtgc gaatctcctc
     5281 accgggggac agcgccgacc cgatgtgggc ccgctgttct atgccccgac gatcgtgacc
     5341 aacgtcgatg agaccatgaa tctgtgccgc ggtgagacat tcgggcccgt ggtgtccatc
     5401 tacccggtct cgtccgcgga tgaggcggtg aagctggcca acgactccaa cctcgggctc
     5461 agtttcagtg tctggtcgcg cgacacacgg cgcgctgagg ccatcgcggt ccgcctcgag
     5521 gcggggagcg tcgccgtgaa tgacggctac gtggcgacct ggggctcgca cgacgccccg
     5581 atggggggtt tcaagcgatc aggacttggg cgccgccatg ggtgggaggg cctgctcaag
     5641 ttcaccgagc cgcagaccgt ggctgtgcag cggttgatcc cggcctacgc cccggcactg
     5701 gggctctcct atgagaccta ccggaagctg gtggagcggt tggtgatggt cttcaggcgg
     5761 ctgccgttct acaagtgact ccgccgctac tcctctgaca tctcggcgag atccgcggcc
     5821 agaatgtcac ggtcctcggt gtcctcgata tcgggaagca gcgccatggc tagctcgaga
     5881 tgctcggtga acgtttccac atcgtcgact tcggcggcgg cccgagcgat cgcctcgtgg
     5941 gcgaatcccc tgacgaaggc gtcgaggtcg tcttcctcgc aaactgccag ggaccgctcg
     6001 ccgaaccgct gtgacagctc ggcgtaaccg agaaccgcgg ccaccctcga tacctgccag
     6061 tcggctatcg cccggttgcg agggtcgccg atttggtacc aatgaaaccg ctgggtgaat
     6121 gtcgacgcga gcatttcctc gtcctcttcc ggggtgcgca catcgaggtc gaggagctcc
     6181 caggtgcgat tgaaggcctg gggagcaaag gtccgatgcc aggccgccga ttgctcgggt
     6241 gtcgggtcct tcactggctc ggtcatcctt ttccctccac tcggggatcg gtccggctta
     6301 ccttaggtag ccaatgagcg accacaccac tgtgattgtc ggtggggaag aggtccggat
     6361 ctccagcccg gaccggatca tcttcccgcg ccggggatgg tccaagctcg atgtcgtcga
     6421 gcacttcgtg gtcgtggcgg agggagcgat ccggggcatc gccggccggc cgacgatgct
     6481 caagcgctac atggggacgg tcgacgagga ccccatctat cacaagcggg tctcgaacaa
     6541 ctcaccgttt gactcggtgc agatccgatt cccttcacaa cggcccggtc ggatgaatgt
     6601 gccccgaact caggcggatg tggtgcgcct ggcccagctt ggatgcctcg actttcaccc
     6661 atggccggtg cgagccgagg acaccgatca tcccgatgag ttgcgcctcg acttcgatcc
     6721 cggccccgga gtcaccttcg aacaggtgag agctgctgcc caggcggcac gcacccttct
     6781 cgacgagatc gggttggtgg gctggcccaa gacttccggg agccggggga tccatgtcta
     6841 tgtgcggatc gagccattgt gggatttctt ccagacccgg cgggcggtct tggccttcgc
     6901 ccgtgagctg gagcggcgga tgccgggcga ggtgaccacc aaatggtgga aggaggagcg
     6961 acaaggggtc ttcatcgatt acaaccagaa cgcgagggac aagtcggtgt cctctgcata
     7021 cggggtgagg ccgaccggtt tcgtatcggc cccgatcacc tgggatgaat tggcgaacgt
     7081 cgcaatcgag gacttcccgc tcgacacctt ccgggaccgc tactccaagg ttggagatct
     7141 cagtgacggg atcgacgagg cggccggacg gatcgacact ctcctggagt ggatggcccg
     7201 ggatgaggag aacgggatcg gggacgcccc ctggccaccc cactacccca agatgccggg
     7261 cgagccacca cgggtccagc cttcgcgaca acgccgcccc gacgaggact actagaggcg
     7321 tttcctgtca ctattctggt aaccggtaat gcggaaactg gaagtgcccc gttcacaaat
     7381 ggtcaagggt ctcgtcgacc tcggtgtgct caccgtcctc aaccacggcg agtcgtatgg
     7441 ctacgagatc atgacctggt tcgagaaggc cgggctcgac gatgtcggtc acgcctcggt
     7501 ttacggatcg ctgaagcgac tggaagagca gggatgtgtg gccaccgagg aacggccctc
     7561 gccattgggc cccgcccgcc gctattaccg gataacgccc cggggtaagg agtatcgagc
     7621 cggcctgcac aaggcctgga gtggtctgca gtcggccctg gagatcctgg agcggggaat
     7681 gtgaccccga gcgagcaata cgcccgagag gttgtctccc atttggagcg attgccgcgg
     7741 gccaagaggt cgcggctccg acgggaattg gctgcggagt tggccgagta tccggaagcc
     7801 gaagaccgtg aggcgttgga agcgcggctc ggaaccccga gtcgctacgc cgctgacctc
     7861 gccgaggaac ttggggtccg acctggacga cgatcccgac cgcggggttg gggttggggg
     7921 tgggtagcgg cggtcttggt cgtcgtcggc tggctcctgt tggcgaattt gaccactcca
     7981 ctcgaaccgg gctccttctc ctcatcgggg agcttcgagg taactgtggg cggagaccct
     8041 ttcgatgagt ggacggaagt cgtctatcac caggatcagg agggagagat gagctcatcg
     8101 ttgcgcaatc gtgcccggct ctcggtgacg atcaccgaca tcggggtctt ccccgccatg
     8161 gaccgttcaa gccgtgagct gacaggttgg tccggtctct ttgtccaaac cggtgagttc
     8221 tatgaaggag acacaccacc ggggtatgga ttcatggacg atccagtctc attcaccgag
     8281 tctccattgg tcctggcgcc gggagaggaa cggacgattg cgattcaggc catgctgacc
     8341 aattgtgagt tcaatacgcc aggtgcctcc gaggggcacg acgccgcgat ggtgacctac
     8401 agctatctcg ggctgaccaa agtggcgaca gtccagttcc ccaaggtgtt caccacctcg
     8461 ccgcaggtct gtgaggctcc tgtcaaccga gagtttgcga ctgccccgga ggctattgct
     8521 gacgggatcg ccccttcctg gatcccgtcc gacgcccgtg acgtccttct cgccaccggg
     8581 gaagcgggct cagtgctcag tttgaccacg gatcagttgg cacccgatac ctgcaagggg
     8641 tcgaacgcga gtccctactg gagttatcgg cccagttggg cgccgtctca ggacctcgta
     8701 gctgccaagg acgtgctggc ctgccccgag gactggttct ggtggggacc cgaaccaggc
     8761 gaggttctga tgtggacaga gactgagtat ccgttcgtgg cgccctagag ggcgtcgcgt
     8821 cgacgaaggg cccaggtgag gaccacgagt ccgaccacca ccacgacggc gatcttggcc
     8881 acgcctccac ccacaccttc ggtgaccggg cccagagtgt cggtcgcaac ctggccgaac
     8941 tcgtcctcca tcccagcgta aatggagagc gagatgcgcc agatcgagaa ttgggcgagc
     9001 ccggtcacga cattggccag gatggtctcc caaacgatta tgtagccgag cccgatcagg
     9061 agtgagcggg gcgccaggta gccgagcgga acgaacacag cggcgtaccc gatgcccgcc
     9121 gccacgaaaa gggtgagagc ggggattgtc ggaccgatgc cgttgccaac cgcaatcgat
     9181 gcgaccacgg tcaccagcca gccgcagatc ccgatcacaa tcgccgccgc cgatccggca
     9241 atgagggcct gtccggcgaa ccaggcacgg gggatcgggc gcagatagat gtagggaagg
     9301 gttccggcat ccttctcttc gcggagggtg gcggcggtga ggatcagcgc ggcgacgacg
     9361 aaggtgtagc ccaccgaggc caggatgtcg gtgtagagcc cggttaggtc attggccggg
     9421 gcatcgaagc cgaccagcca atacaccagc cccgagaccg aggctagggc aacgagggcg
     9481 atcacccgcc cccggcgcat catccgccgg tagagatgga gggcaagagt catcaacggc
     9541 cctcgaccag gtagcggaac actgattcca gggactcgtc aaccggttcc acccgagtaa
     9601 gacggattcc gccctcagcg gcgaggatcg gcaaccgcct ggccaggtct ccggagtcgg
     9661 aagcctcgac atgaacggtg tcgccgtcga tgctcacccc gaccacaccg gggctggcga
     9721 tcagcgacga cgccagacgg cgggggtcgc tggcttccac ataaacccgg cggggcttgt
     9781 cggtcatcgc ggcccggatc gtcgaaacgt caccaacagc ggcgaggcgc ccgtcaacca
     9841 tcgccaccac ccggtcggtc attcgctcga cctcggcaag gacatgcgat gacacgatca
     9901 cggtcttgcc ttggtcgccg agtttccgga agagatcgat gagatgagcc ctctgcaccg
     9961 ggtcggcgcc gttgagcggc tcgtcgagca ataagacctc agggtccgac acgagtgctt
    10021 gcgccacttt cgcccgttgc ttcatcccct tggaaaaccc accgagggcg cggtcggcgg
    10081 cgtcggtcag gccgacatcc tcgatggccg cgctcactcg ctccttgacc cggtcgacct
    10141 tggccaggcg ggccgcaagt tccacgaact gacgaccggt gaggcggtcg tagacggtct
    10201 cgtcctcggg caccagcgcg atccggttgt agatggacgg atggtcgcgt ggatcaaggc
    10261 cgaggacctg aatgcttccc tgtgagggtc gttgcagccc ggtcatgacc cggagcaggg
    10321 tcgtcttgcc ggctccgtta gggcccagga ggccggtcac gccgggtctg aacccgatgg
    10381 tgagctcgct gacagcgacc ttgagcccga accacttgga caactcggtg acaacgatgg
    10441 tgtcggtcat gccagtttcc ggtaacgcca tctgatgaac cacaccgcgg cgacggtcac
    10501 gccgacgacc accgcgaccg acacccagac atcgaagcct gcgatctccg ccgggtaggc
    10561 gatcgtgtcc ccgaagaagt aatccctgat gatccgcggg tgctgacccg gggcaaccag
    10621 cgaggcgaac ttggcgccgg gaaacgtcgc ctcagcgacc cgtccggcga tgccgtctcc
    10681 ggcgaccagg attcccagaa aggctgcggc cgcaattccg gtgcggtcga tcaccgatgc
    10741 caggatgaag atcagtgaac cgtgaagggc gacgaatgcc agggtggtca cgggcacctt
    10801 ccaaaggacg tcgaggttgt cgcccaggta ggtgagaaat ccatcgtcgg agagcaggga
    10861 cagcccgaga tggagtatca gctgagggac gatgtagatg gcgccgacca cggtggcaaa
    10921 cgcccccacc ttcgaggtga ggtacccatc aacggtgaga ggtcgggaga aatagacgga
    10981 aaggactccc ttggtccgat ccgggatcag caacttggga ccggcgaacg caatgaacag
    11041 gatggaaatc gccgagtaga agtcgaagag gtcgccgtag gtgggggcac cctcctgaat
    11101 cgcctcgtcg atgttgccgg cggcccagtt gatgccgaga aaaaccgcag ccgcaatcac
    11161 cgcgatggca atgaggaccc aggggaagat cttctcccga gcctttcggc ccagcccgag
    11221 aacccggcgc accccgtctt tgaacaccgc tcgccgggca ccggccggcc ctagtcgtgg
    11281 gccgtcgtac gtccgatagc cccggtcgaa gatgacctgg tcggtcattg agacgcctcc
    11341 cgggccagga acaggtcctc cagcgaggcc gatccccgga tcatccggac caggccggag
    11401 ttcgctgctg cggtttcggc gacgatcgcc tcctccagct tctcatcgcc cggcccaacc
    11461 agcaggcgct gaccctcgac cgtcgcttcg aagccgcgac gtctcaatgc ctcggcaacc
    11521 ttgtcggcgt gatcgatcac ttcgacgacc acggtgccca gtttctcgaa gccctccagc
    11581 gggccagagc gaagcaggtc cccggcgtcg agcatgacca cccaatcaca ggtctgctcg
    11641 atatcggtca gcacatgaga ggacagaatg acgttgacct tgaagtcggc gagccgtcgg
    11701 atcagctcaa gcatctgtgt gcggccctcg ggatcgagcc cggaggccgg ctcgtccagg
    11761 agcagcaggt ccgggtcgtg aacgatcgct tgcgccagtt tcaccctttg ctgcatgccg
    11821 gttgagaagt cacccaggaa gcgaaagcgc tcctcctcca ggccgactag gaagagggtc
    11881 tctgaggctc ttcgcctcgc ttcacgcggg ggaattccgg ccaattgcgc ggtataggcc
    11941 acgaagtcgg cggcggtttg gtccttgggg agacaatcgc cttccggcat gtagccgatc
    12001 acagtgcgga ccgcgagagg atctcgctgc gggtcgtgac cgagaacctg catcgagccc
    12061 gacgttgggt ggagcaggcc gagcatcagc cgcaacaagg tggtcttccc cgcgccgttg
    12121 gccccaacca gcccggtcac tccctccggc acctcgaggg tcaggttccg gatcgcaacc
    12181 acctcgccgt aatcgtgagt caggtcagag acttctagaa cggtcatccc gcaaccacct
    12241 ctgcgaaccc tggcccctcg gggcgctcca gttgctccat cgtgcagctc aacggatcct
    12301 tctccgggcg ccagcggtga aacctggtgg cgtgtctgaa tcggttccct tcgagttggt
    12361 catagctcac ttccacaacg acaccgggtt gcaccggggt ccaagctcca tcctttgctg
    12421 acgaccatcg actctgccct cccggcgccc gggcatcctc gtcgaagctg tcggcggcca
    12481 ccaattcgag aaagcggttg tagatctcga ccctttgccc atcggggaag ccggagcaat
    12541 gtccgatgaa gtgaaactga ccttgcccgt cgtaaacccc cagcaagaga gagccaattt
    12601 tccccccgtc cttgtgctct ctgaaccctc cgacgacaca gtccacggtc cgccggtgct
    12661 tgatcttgat catcgcccgc ttcccgtgct ggtatgcgag gtccggcaac ttggcgatga
    12721 tcccgtcgca accggccgac tcgaactcgg agaaccatct ggtggcagtt tcctcggatt
    12781 cggtttgcgg agtcaggtgc caggggtgcc cgagatcggc cgcgacactc atgagtcgct
    12841 cacgtcgttc cacaaatgga gcagctcgga ggtcctcccc ctggtgggcg agcaggtcga
    12901 acgcgaccaa ctcggccggc gtcgcttccg agagcatctt gattctggat tcggcaggat
    12961 ggattcgctg ctggagggtg ccgaagtcgg ttgccccgtc taccaccacg atgacctcgc
    13021 cgtcgatgac ggtgccgtcc ggaagctgtt ccaacgcagg gcgcaactcc gggaaatagc
    13081 ggagcaatgg gcgctgcgat ctcgagtcga gtcgaatgtc gggttgggac caggagacgg
    13141 atcgaaaacc gtcccacttc ggctcgtaga ggaaggttcc cttcggccag ccctccgaca
    13201 ccttcgcttc catcgtttcg atcggcgccg ggaaagggtg actcacgggc ggcgagtgta
    13261 tgcgccgata cgctggtctc attatcgagc ggattgtcga gagacttccc aagtgggcga
    13321 aaggcccggc ggagatcgtg gccacctccg ccaaggagta cagcatcgat cgcgccccga
    13381 ggatggccgc ggccatcgct taccggactg tcttcgcctt ggcgccgatg ttgatcatcg
    13441 cggtttccgt cgccggtgcg ttccttggga atgccgaggt tcagcgcgaa gtgctggaag
    13501 cagtcgagcg gatcgccggc ccgcaggtgg caaccacggt ggccaccatt ctcgccaccg
    13561 ccgtgagctc caccaacacc gcggcaatca tcggagtcgt cttgctgctc tggtcggcct
    13621 cgagtctctt tatcgagctg caacatgacc tgaacgacat attcgatgtc ccctatgagc
    13681 gaatctccgg cctgatcggc ctggtccgaa agcgggggat cgggtttctc tgggtgttcg
    13741 ggttgggttt ggtgatgctg gctctgctgg tgataaatgc gatttggggc ttcctcgact
    13801 ccctgctgcc ggagagcctg gagaatctgg acactgtctt ccaggtgttg ggtcccattg
    13861 tctcgttggc gattctgccc ctcgtgttcg ccctcatctt ccagacactc actgtggtgc
    13921 cgattccctg gcgtgcggtc tggttcggcg ccctgttcac tgcagtcgtg ttcaccgtgg
    13981 ccgcttacgg gatcgggatt tggttccaga tattcggggt caccaccgca ctcggattcg
    14041 ctggctccct ggtggtcgtc ctgttcatgg cgtacatgct ttcgtccgtc ttcctgtttg
    14101 gggccgaagt gaccagggcc tacgcggatc ggctggccgc ggctcgggtc aaggctcctc
    14161 aacgaccggt ggaccccctg gttgtcgtgt ccgagccgcc ccgggccata cccggcgcgg
    14221 cagtggtcgc cttcctcacc gggttggtca tcggctggcg tcgtcgcgat cggtagtcgt
    14281 ctcactgtga tttgaatccg gagtccaata acctcagtca tcaatgtcga tcgacatcgc
    14341 aggctttgtc accgatctga aggaacatgc caccgagcac ggatttcacg tgcatgacga
    14401 gcggcatttc gtcgagacct actcactgcg ccagtcctgg gaggtggatc tccatccgga
    14461 ggctggttgc ggcgggccca tcgatcttca cctggcgctt gacggtgacc ccagggtgat
    14521 tctctctttg cgggagcgtc tcgaggagat ggacgatgac ttcgaggagc ctgaggacgt
    14581 ctaccacctc gacctcttct tcaactgggc ggttccgccg ctccacaacc cgccggacct
    14641 gctggttctc gccaccgatc tcgcgggcat cggccggctc tcgctggtgg tggaggtcca
    14701 ggcaatcgac tcttatgggg cagtcactga cgcccctgag cggaggctga ccctcaccgg
    14761 gaagtcatcg gtaagcctgg tcgacgtgct catgggccgc gagcaactat gtgacgtgct
    14821 cgaccggtcc catgacgtca gcgtctatct cctggaccgg gttgccggct ggatggagct
    14881 gccgacctga tcagccgata aggctgaccg ccgcccagac cccgagggca agtgttatcg
    14941 cgcagagcgc atagaacccg accactgtga ggaccttggg ggaggggcct tctgccacat
    15001 cgaacacacc gttgtcggct tcggaatggg ctgcgctggt ctctgtcagc gcgtcggcgt
    15061 cgattatgag atgtccggcc agtccctggg cgaagcggga ggggtcgtcg ggcaggaaga
    15121 agaccgaatc gtcctcggct tcgatgaccc aactgccatc gcccttctcg acaaaggtgc
    15181 attcggccgt gggccattca ccaatttcca cttcacccga gatgagggtg atcttgtcga
    15241 tgtcgatccc caccatgacc ggcacccggt catcctcgaa cgtaactgtt cccggaaaag
    15301 tcgtcactct cagctccccc cagtggttgg aacacccata agttaggccg ctgccggcgc
    15361 gtcgcaaact agaacggaag accgatcgcc ttgcatacga aagccgtgaa atcggacgct
    15421 ttggagaaca tcgcgatcag acgggtgtcg aaccccgccg aagcgacaaa ttggtctgac
    15481 atcctggtcc ccgcgacgaa gctcttcatg cgcaggtcgt cggcataggg gtgatcctga
    15541 tcgaattccc ggggcacctt cttcaaagtc tcgtcctcgt cctgctcgat cgaccaggtg
    15601 ttggtaaagg ccttgccctt ggtggcttgc ttccagcctg aggggtcatc gttgatcgcc
    15661 tgccgtatcc gacgagcgac gggggtttcg ggatgccaca tcccgacccc tgcgaaacaa
    15721 gcatccggct cgaggtgtac gtagaacccg ggagcgtgga tgtccttgcc ggcatcgtgg
    15781 cgaaactgga tcccaacatt cgtcttgtac ggcgtcttgt ccttcgagaa cctggtgtct
    15841 cggtagggcc gcatcaggga gccgccgatg gttcgggcgt cggctgtgaa gtggggtgaa
    15901 atccgggtca gccggggccc gaaatcctgg atgaagtcca gcgctggctc ccgaacgact
    15961 tcgatgtacc gctccttgtt gtggtcccac cagatcttgt tgttgttgcg gctcagctcg
    16021 gtcaagaagt caaagacttc cgatgaaaag tattgcttgg cgatgacaga ccccctctcg
    16081 gctcgaacct acaacccagg ctgagctgtt ggtaggggca cagaatcaca ccggggtgtc
    16141 ttccttagcc agctcgtgcc acggggtgcg gagcccttcg cgcaccacca caaccagctt
    16201 gcggaagtcc ttgcgcaaca cccaggccgc agcgagcagg aagacgatgc ccactccgat
    16261 ccgggcctgg gctgacccct cctcggatat gtggccgagc tccgccagac ctgactccag
    16321 gagttgtgca acaaataggg cgaacatggc ccatgcctcc ttagtggtca ccgatcggct
    16381 ggcgacgatg gcgaccgcga agaccgactg agcggctgtg accatgagct cgacccgctg
    16441 ctgtcggtcg aggggcagcc cgtgggtggt cctcgaaaag atcgagaaca cgatgggaag
    16501 cgtgccaacg agcagcgtcc actggttcac cttcgaggag atgagggctc cgaacccggt
    16561 tcgggccgca agccgccagg caaatagagc agcgaccaac agttcgggcg cctccgaagc
    16621 cagtggcgcg atccatttca ccaggaggaa ctcgctcacc ccgagcgtct ggcccatttc
    16681 gacaatcgcc tcggcaaacg gctcggcaca gaggaggatg actccagccg ccaccacaaa
    16741 cccgatgtag ttgatgaagc gacgcaatcg tgtgggcagc gacccgatgt aggcagaagg
    16801 cccgaccagg tggggggtcg agggcggggc ccccgacagc cgacggaggt agaggacata
    16861 gatgaagacc aggatcccgg catcccacaa cgtcagtgtg tctttgagaa acagggtcaa
    16921 gccatacaag gaggcgattg tgaggtatgc gatctcaacc gactgcgatc gatcgaggtt
    16981 gattgtcgtc gcggtcgttc cctcggcgcg ctccggagag gcttttgtct tccgtacccg
    17041 gtaagtgccg aggaggatca ccagtggcca accaaagccg atcaacagtt gattgccacc
    17101 ggtcatgttg gccaacgcca agggcccgta ttgggtcgcc gctccggttt cggcgaactc
    17161 ctggcctgcc ttggcggtga acacgaagtc gacggcgtat tcggggagga tcgcaatcag
    17221 ggccaagagg gcgagggcga ggccggccgc gatgtccaac tggatgactt cggccgccca
    17281 ggagaggatg aaggcggacc ccacaatcgc caggccgaac aggaacgccc cgaccaccgg
    17341 cgctgggtga gtgccggtca accgaaggaa gaccccaggc agcgtggccg caacggctag
    17401 ccctatgtag aggatctgac ggcggggatt tagatcgacg ctttccatcg ggtccgtggg
    17461 gatcttaagt gggccgcggc ggggtcagtt caagactctt cgacatgacc atggcttcca
    17521 aaagccctgg cccagatcct ggtatctctg ccccagccag gccgccgtcc cgatattggc
    17581 caggctgtcg aatgggccgc tgttggggaa acctgccttc gaagccgttg ctgcccaagt
    17641 agacgggagg aattggaaca ggcctgaagc ccctgaatag gggttgtagg cctcgggatc
    17701 tcccaggctc tcacattgga ttatcgccag cgcctcgttg actcgagagg cggggaaata
    17761 acggctgacc tcgtcgcgcc agcgctccac agccgccggg aaattggtgc tcccacctcc
    17821 gcctccgctg tcgtcgtcgg tggtcgtcgt gactgccacc gtggtagtcg tggttggggc
    17881 gttcgtggag ttcggggtgt ccggagtggt ggtcgttggc gggtccctct cgtcctggcg
    17941 ctcctgctcg gcctccctcg cccgcgccgc gtcaacggcg ttgagagcct cgctgtattc
    18001 ggccgcggac ttgctggcct cgcggatcgc ctcggccacg ctggcgtcag cctgctcgta
    18061 gagacttgcc aggttctcgg tctcggcatc cacctcggct tgaagctcag cgactcgctc
    18121 ttgatcggca aggaaggttg ctaccagctc ttcgaggtcg cgtcgtttca tgacaagagc
    18181 gttgacgttc tcctgcccgg tcgcgacgac gtcgccgatg aagaggccgg caaccagcgc
    18241 atcttcaacg ttgtcggagt tggccaggct gactccgggg gagctgaggg cgctcatgta
    18301 ggcatcgacc gcctgggtct cgatatcagc cttgatcccc aacaattcgg cgtcggcgaa
    18361 cccgatttgg ccctggagcc tgtcgactcc ggccgcgaca gaggagagtt gctccgccag
    18421 ttcgtcgagc ctgaccatgc tggccgccaa ttgggactcg atgtccgagc ggttggctag
    18481 ggcctcgtcg acgagcccac tggccgcccc ggcctgctgt tgggcctctt cggctgcgtt
    18541 ctcggcgtcc ccgacactct gggcgcgcgc cccatcagcg gtgctgatga gcacggctgc
    18601 ggccagaacg gcggccagaa gcgaggagag ggtgcgacga cagcgcatcg gttgggaggc
    18661 aagggtaacc aaagctctca gccacgtgaa gtggggaccc aggggtttgg gcggacaggc
    18721 cgagttcgcg aggtttaagg tcggctatcg accgtctgaa catctcctcg ggctcgccat
    18781 tcgagccact catcggtttc agccgtgccg tccgggttgg cgatcgagtc ctcgtggcgg
    18841 ggactgcgcc gatctggccc gatggcgagg tcgaccccga tcccgcgaca caggcgaggc
    18901 ggtgtctcga gatcattctc gatgcgctgt accaggcggg agcccgtgcg agtgacgtgg
    18961 tacggaccag gtcgttcctc accgacgcct cctatgcgga tgctgtcggg agcgctcacg
    19021 gtgaggtgtt cggtcaggtg agaccggcgt cgacgatggt cgtggtggcc gggctgctcg
    19081 acgagcggtg gaaggtcgag atcgaagccg aggcgatcgt ttccagttcg gtctaggtgg
    19141 tctcagccac cggccgagaa tgtgtcacag gccccgggat cccctgagtc gtagccggtc
    19201 atggtccact gtgcgcgttg ttcagccgat ccatgggtcc acgtctcagg gttgatctgg
    19261 cccgtcacct gactctggat ccgatcgtca ccaaccgcct ccgccgcctc gatcgcctcc
    19321 tggatctctc ccgggtccag ctctactgac tcgtcggttc cggtcccaac cgcaagggtg
    19381 gatgcccaca ccccggcaaa gcagtcagcc tgcaactcca tgaggatcga aagctcgttg
    19441 gcctggtcgg gattctgttg ttggagcgct cgtacctcgt cggagactcc gatcactgtt
    19501 tggatgtgat ggccgaactc gtgtgcgatc acgtatgcag gggcgaaatc gcctcccgca
    19561 ccgaactgat cctccaagag ggtgaagaag tcaaagtcga ggtagatctt ctcgtccacg
    19621 gggcagtagt gaggcccgac cgtctcgtct gcgccgccac acccagagtc cgtgtagtcc
    19681 tcgaagatca caaacccagg cattctgtag tcatcccgcc ctgccgcgat gaagacatcc
    19741 gtccacatca ggtcgaggtc ggtgtacacc gcgttcatgt agtccttgag gtcggcgtca
    19801 gggtcggcag tgggagcgat cgtgccctcg ttttcgggag ccaccacgcc aggggcaagg
    19861 gcatcggtga tgtcgtcaag cgctcccgga tcgattccac cgccgccgag aaacatgacc
    19921 aataacacga cgatcagccc gatcccgcct gcccctccgc cgagtgcgat tccacggcgc
    19981 gaagtggttt gaccccgccg gtcttcgagt cccttactga ctggctcttt cttgtaccgc
    20041 accatgagga ctcgaggaaa ctaggccgat cgggaacgcg tggccaaacg atcgggttgt
    20101 atcggccagg aggtcgatcg agatgtttgg acgaaagaag agcgagatga caggtgcgag
    20161 cgatgccctt cccgggaggg accggtcagt ggtcgtgccg cccacccatt tcgtgaatgg
    20221 gaatccgttg accgcgccct tccccgcgga caccgaggtg gccgtcttcg ggatgggctg
    20281 cttctggggg gccgagcggc gattctggaa gatggacggc gtctacagca cggccgccgg
    20341 gtacgccggg ggctataccc cgaatccctc ctacgaggag gtgtgcaccg gccgtaccgg
    20401 ccacagcgag gtgacgttgg tcgtttatcg gccggccgag gtgtcgtacg aggaactgtt
    20461 gcgcaccttc tgggagaccc acgacccgac gcagggaatg cagcagggca acgacatcgg
    20521 cacccagtac cggtcggcga tctacaccac caccgacgag caattggaaa tagcgaagcg
    20581 gtcacaggac aagtaccagg cagagttgac acctcttggc ttcggtgaca tcacaaccga
    20641 gatcgcgcct ctgggagagt tctactacgc cgaggattac caccagcaat acctggcgaa
    20701 gaacccgcac ggatacgact gccacgccaa caccgggatc gcctacccga tcgcctgact
    20761 gagttcagct ggccctgagt gaggcttcca gggccatcct gacaacgctg tccacggcga
    20821 gcccaccatc gatccgctgc tcgacgtcgg ggctcacgcc ggtttcgccg aagtcgagcc
    20881 cgccgggtgt gacccagcgt gcgacggtca gcttgagcgc ccctccgttg gagagcccga
    20941 atcgctgctg aaccgtgttc ttgccaaagg tgttctcgcc aacgacggtg gctcttcccc
    21001 gctcctgaag gacggcggag acgacctcgg aggccgaggc gctgcccttg ttgacgacga
    21061 agacgacgtc caggtcggag ggcaccaacg gatggcccga cacctcgtag gaggtgctcc
    21121 cttcgggtcc ctcggtgacg accacatcgc catcggcgag gaataccgac gtcacggcga
    21181 tcgcggtgtc gaggaggcca cccgggttgt cccggaggtc gattacgagg gagtcggcgt
    21241 cagccgtgag caaagtggtc actgcgtcct cgaactgctc gtctgcgtcc ccggtgaaca
    21301 ggttgagtcg cacgtatccc gtatgcccca cgagctggct ctccacgacg ggaatgacca
    21361 cgtcggctcg gatgatcgtc acatcgaact ggtctccatc gcggagcacg gtgagggtga
    21421 cctcgctgcc ggcctgaccc ctcaccgtcg aggtcacctc gtcgacagtc catccgtcaa
    21481 cgtcggctcc gtcaacgcgc aggatcacgt cgtcacgcat cagtccggca gattgcgcag
    21541 ggcttccttc gatggtcgag acaatgagca ggtggcaggt cgcggagacc acttggcact
    21601 gtgccttggt cccagtcgat gagtcctcgg caatgaccag cgcgcctatc ccatggatct
    21661 cgccgtcctg ctcctcctcg agcagcgcca gcgactctcg atcgaggtaa accgagttgg
    21721 catcaaggcc ataggttgcc aagcccgcca ccatcgcctc agcggcctcg gaagtggttt
    21781 cggcctcgtc gacgatcgac cgacaggttg catcgaactc atcggttggc tcggcgcaga
    21841 ccagggcgct ggttgaggtc gatccgtcga gatcggccac ccctagggct gcggcacccg
    21901 ccagatcgac gtcggccgga tcatcgacat agtgacgccg cacgagttcg taggcctcac
    21961 agacgatctc gacatcttct gtcgcctgat cacaggtgac ggtctcgagg tcgcgccggg
    22021 gcggttcagg gctggccgtg ggggtagtcg aggaggtgga gacaggttgg gtgccttcat
    22081 tgccagccac ttcagtgcaa gcggcgatgg tgaccacgag ggcaagcgcg acgaggcgca
    22141 aggggccctt caaccggacg cctccacaga cagatcgacc agttcttcct tggtcaggcc
    22201 ggcaggaagc gaaacgaggt gatcaggggc gataccagtt tctgccaccg agtctccatt
    22261 cgggctcgtc cagtgagcga cggccacata gagctcgcct ccgttccgaa gctcgaaggg
    22321 tatctgcacc gcattcttac caaaggtggg ttcgccgacg atgacggctc cccgacggtc
    22381 ccggagggca gtcgccgtga tctcggcagc ggatgcggtg ccctggttca cgaggacgat
    22441 gaggtcgact ccggtggcca atccgccgcc cacggcggtg acatcgttct ccgcttcggg
    22501 gtcggaggtg tggaaaatga caccgtcctc gacgaattcg gagatcacca gaaccgcggc
    22561 gtcgacataa ccgccggggt tgtcccgaag gtcgacgatc agggtgtcga tcccgtaatc
    22621 gatgagatcg gtgagaccga ggtggaccag gtcagggatg tcatcctcga agtccgggat
    22681 gcggatgagg ccgacccggg gtcggggggc gtcgatttcg accgtgggaa tggtcagctc
    22741 atcgcgctcg atatcgagga catccgactg cccatcgacg cgctggacgg ttatggcaac
    22801 ggtccccgtc tcatcgcccg cgatctggct cacgacgtcc acgaatccca tgccgtcgat
    22861 gctcgtgccg tcgattgcgg tgatgacatc tcccgccaaa aggccagccg cctcggccgg
    22921 gttgtcttca acgacgaaca cgacccgcag cgggcacgag gccgtcactc gagcgcaatg
    22981 tgagcccacg gcatcggtgg cgtcgaggag ggccccgact cctccaacca ccccgttgga
    23041 tcggaaggcc ccgacctgat caggtggcac gtaataggag aactggtcga gaccgtgatt
    23101 ggccatctcg ctcacggctg cttcaaccgc ggggccgatc gggatcgact ccgagtcaac
    23161 cctgtcggcg aggtgttcgc acaagtcacc gaatgcagca tccgggatcg cacacatcag
    23221 ggcacggggc ggatcggcgg tctggtcggt tgcaaagttg tcgagcccct ctaaagccgc
    23281 cgccgccagc gctgtcgcgt cgaggggtcg atcgacatgc cattcctgga tcagctggta
    23341 ggtctcacag agagcagaaa acgagacctg aggcgccgag caatcatcca cctgtaccgg
    23401 aagcgagggt gtcgtcgtcg ttggtcggag agctgtcgtg gtcggcgtgg cggcggtcgt
    23461 ggtcgtcgct tgacccgcgc ccgaattgca ggcggaaacc aggagagcgg cgacgaggac
    23521 cgacttga
//
LOCUS       DBNHXU010000281         3125 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2671844, whole genome shotgun sequence.
ACCESSION   DBNHXU010000281 DBNHXU010000000
VERSION     DBNHXU010000281.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2671844"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..302)
                     /locus_tag="AC161X_07165"
     CDS             complement(<1..302)
                     /locus_tag="AC161X_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013808469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR4989366.1"
                     /translation="MRALVKHADERGLRLDDVETPSIGADDVLVRVHTTGICGTDLHI
                     YNWDDWAQATIPVPMTIGHEFSGTIVELGSNVSGFEIGEVVGAEGHVVCNRCRNCLA"
     gene            complement(299..1366)
                     /gene="add"
                     /locus_tag="AC161X_07170"
     CDS             complement(299..1366)
                     /gene="add"
                     /locus_tag="AC161X_07170"
                     /EC_number="3.5.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF013155.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004000 - adenosine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0019239 - deaminase activity [Evidence
                     IEA];
                     GO_process: GO:0043101 - purine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HIR4989367.1"
                     /translation="MPLPSVLLHDHLDGGLRPDTVLELAEEHRYRDLPASNAEDLAAW
                     FHQTDSGSLERYLEAFTHTIGVMQQPEAIERVAYEAAVDLSGDGVVYAEIRFCPSLNT
                     REGTSVETVIQAASSGLAMGGQETGLKWSLIVDALRHWEDSLELARVAAASRDLGVVG
                     FDLAGPEAGFPPADHLPALRLARAEGLRVTIHAGEHAGGSGVAYMASAMDVCGAERLG
                     HGVELVEDCLLEDGEIVKLGKVAQRIRDRRMPLEMCPASNLATSGISAEDHPVGAMYR
                     AGFNVTLSTDNRLMSATSMSDEFDFVTKHHGFDITDLARITWHSLDAAFCPWETKAAL
                     WEDIIAPGFAAAGADIERAWR"
     gene            1420..2676
                     /locus_tag="AC161X_07175"
     CDS             1420..2676
                     /locus_tag="AC161X_07175"
                     /inference="COORDINATES: protein motif:HMM:NF024578.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HIR4989368.1"
                     /translation="MPKAHVIVLTAVLLGACMTAPPPRDALPEVEEVLEPPVACSFLV
                     GPPQGVSVAEVLEDTGAEGVLKAGDVITSLNGIETADQDGLRDALAEESVGDSIHLEL
                     VRNGNEETADMVLGANPEDPERPFMGVMIRTAYEHLDPTDADDAIPDVDTARAVVIGG
                     FLFGVEPEESLWSGTGLGVSNETNWVATSKSVYGLGVGDERILTDLISGEVINYPVVD
                     GWLPARLIGSIDDDLLLAVTQEVPDQPDLVSVATSRFDPETGDTEWIEPISEGFGVPV
                     TAWASPDGNYITIGGIEANTTALTGVELLTSDGVTAGLDELLSLGTPIGWLDEETAVF
                     RTSATTVSKVNAVTGEITEVTLDSALEGVPVYAVADGHSVLAVSDRSLLMDDLATDGE
                     LRLLAENCSIGRVGDAGWRPTSDQSA"
     gene            complement(2664..>3125)
                     /locus_tag="AC161X_07180"
     CDS             complement(2664..>3125)
                     /locus_tag="AC161X_07180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989369.1"
                     /translation="LMEGSWGMGKTISSTSGTRSNESGGPANPVSTALRERKEQIEER
                     LTVLYNRVALLLGEHEDKILELAAVLEEKKQISGEEVAEIMGSDPGSRTMRQPKGWQA
                     MSDEVAEVRSRDALTKAGRTSGAAPSNGKPAGAEAGEPEPEPEEVEAGQAD"
BASE COUNT          553 a          997 c          963 g          612 t
ORIGIN      
        1 gcaaggcagt tgcggcatcg gttacacacg acgtggccct cggcgccgac cacttctccg
       61 atctcaaatc cggacacgtt ggagccgagc tcgacgatcg tgccggagaa ctcgtgcccg
      121 atggtcatcg ggaccgggat cgtcgcctgg gcccaatcgt cccagttgta gatgtggaga
      181 tcggtcccac agatccccgt cgtgtgaacc cggaccagga cgtcatcggc gccgatgctt
      241 ggcgtttcga catcgtccag acgcagcccg cgttcgtcgg cgtgcttgac cagtgctctc
      301 atcgccacgc cctctcgata tcggccccgg ccgcggcgaa tcccggggcg atgatgtctt
      361 cccagagggc ggccttggtt tcccaggggc agaaagcagc gtcgagactg tgccaggtga
      421 tcctggcgag gtccgtgatg tcgaacccgt gatgcttggt gacgaagtcg aattcatcgc
      481 tcatggaggt ggcgctcatc aatcggttgt cggtgctcag tgtgacattg aaaccggccc
      541 gatacatggc tcctacaggg tggtcctcgg cggaaatccc gctggtggcc agattcgagg
      601 cggggcacat ctcgagtggc atccgtcgat ctcgtatccg ctgagcgacc ttgcccagct
      661 tcacgatctc cccatcttcc agcaggcaat cctcgaccaa ttcgacccca tgacccaggc
      721 gctccgctcc acaaacatcc atcgcagagg ccatgtatgc gactccgctg ccaccagcgt
      781 gctcgccggc gtgaatggtc acccgcagtc cctcggcgcg cgccaatcgg agtgccggaa
      841 ggtggtcggc cggagggaag ccggcctcgg gcccagccag gtcgaatcca actactccga
      901 gatcacggct tgccgccgcg accctggcca actcgagact gtcctcccaa tgacgaaggg
      961 cgtcgacgat cagactccac ttgaggccgg tctcctgccc gcccatggct aacccggatg
     1021 aagccgcttg gatcacagtc tcgacgctgg tcccttccct ggtgttcaga gatgggcaga
     1081 accggatctc tgcgtagaca acgccgtccc cactcaaatc gacggccgcc tcgtatgcga
     1141 ctcgctcgat ggcctcgggt tgctgcatga ctccgatggt gtgggtgaac gcttcgagat
     1201 agcgctccaa tgagccggaa tcggtctgat gaaaccaggc tgcaaggtcc tctgcattgc
     1261 tcgccggaag atcccggtat ctatgctcct cggccagttc caggaccgtg tcggggcgca
     1321 acccaccatc gagatggtcg tgcagaagca ccgaaggcag gggcatttcg tcacgctagt
     1381 cacgagggat atcaagccgg ccaccggtac gctgtcgccg tgcccaaagc ccatgtgatc
     1441 gtcctcacag ccgtgctgct cggtgcgtgc atgacggcgc cacctccgcg cgacgctctg
     1501 cctgaggtcg aggaagtcct cgaaccgccg gtcgcctgct cctttctggt gggcccgcca
     1561 caaggcgttt ccgtggccga ggtattggag gacaccgggg cggaaggggt tctgaaagcg
     1621 ggggacgtga tcacttccct gaatggcatc gaaaccgcag atcaggatgg tcttcgagat
     1681 gccctggccg aggaatcggt tggcgactcg attcatctcg aactggtccg caacgggaac
     1741 gaggaaactg cggacatggt tctcggggcc aaccccgaag atcccgaacg cccgttcatg
     1801 ggagtgatga ttcgaactgc ctatgaacat ctcgatccca ccgacgccga cgacgcaatt
     1861 ccggacgtcg acactgctcg cgcggtggta atcggcggtt ttctctttgg tgtcgagcct
     1921 gaggagagcc tgtggtcggg caccggattg ggtgtttcca acgagacgaa ctgggtggcg
     1981 acgagcaaga gcgtttacgg cctgggggta ggagatgagc ggattctcac cgacctgatc
     2041 agcggcgaag tcatcaatta cccggtcgtc gatggttggc ttccggcccg cctcatcgga
     2101 tcaatcgatg acgatctctt gttggccgtg actcaggagg ttccggatca gcccgacttg
     2161 gtctcggtgg ccacgtcgag attcgacccg gagacaggcg acactgaatg gatcgagccg
     2221 atcagcgaag ggttcggggt tccggtgacg gcctgggctt ctccggatgg caactacatc
     2281 acgattggag gcatcgaggc caacaccacc gccctcaccg gcgttgaatt gctcacatcc
     2341 gacggggtga cagccggact ggatgaactg ctctccctcg gcacgccgat cggctggctg
     2401 gacgaggaga ctgccgtgtt ccgcacctct gccaccacgg tttcaaaggt gaatgcggtg
     2461 actggtgaaa ttacggaggt gaccctggac tccgcgttgg aaggtgttcc cgtgtatgcc
     2521 gtcgcagatg gtcacagcgt cctcgctgtc agcgaccggt cactgctgat ggatgacctc
     2581 gccaccgacg gcgaactgag gctgttggcc gagaattgct cgataggaag ggttggtgac
     2641 gccggctggc gccccacgtc ggatcagtct gcctgacctg cttccacttc ctccggctcc
     2701 ggctccggct ccccggcctc ggccccggcc ggcttcccgt tgctgggggc cgccccggag
     2761 gttctccccg ccttggtcag cgcatcgcgg cttctgacct cagccacctc gtccgacatc
     2821 gcctgccatc ccttgggctg gcgcatggtg cgtgatccag gatcggatcc catgatctca
     2881 gcgacttcct cacctgagat ctgcttcttc tcctcgagaa cggccgccag ctcgaggatc
     2941 ttgtcctcat gctcgccaag caagagggcg accctgttgt agaggacagt gagacgctcc
     3001 tcgatctgct ccttccgctc gcgcagagcg gtggaaaccg ggttggccgg tccgccgctc
     3061 tcgttggagc gggttccgct ggtcgaggaa atggtcttcc ccatgcccca cgagccctcc
     3121 atcaa
//
LOCUS       DBNHXU010000282        14897 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5452658, whole genome shotgun sequence.
ACCESSION   DBNHXU010000282 DBNHXU010000000
VERSION     DBNHXU010000282.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 14897)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14897)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14897
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5452658"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..2684
                     /locus_tag="AC161X_07185"
     CDS             132..2684
                     /locus_tag="AC161X_07185"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HIR4989370.1"
                     /translation="MARKALIHQIEARERRGRWFGALATLLSMSLLLAGWVGLFAFLG
                     TNSAYGTFEGIKDDWVPDTRSMELTLPDLSRVSRIYADSGEVLAELHDGRNSEPVPYE
                     EVPEVLVHAILAAEDSEFFEHSGVDFSAIVSAAIDNLIYDTTRGGSTITQQVVKNAFV
                     GSEVSIRRKINEAFVSAELERRFPKERILEYYMNSVYFGAGAYGVQTAAVEFFGKELN
                     QLDASEAATLAVLVRNPSLYNPRRRPEITLERRDDVIREMEQDGWLTTAEADHAVLQP
                     LGVIEAPLRRGEADHVVAEVKRQLLNSDEFAFLGATPEDRKKAIFGCPADDVACDGGG
                     GLQVFTTIDLGLQKKANEILASWMPLPPYEQNVAACKGILPNETDAFLATYAETHSCV
                     PTGAMTMVDNHTGAVKVMASGLPFEFSQFDLAVQGRRNPGSSFKPFGLVAALEQGYTM
                     GHTWSGESPIEIECPFPCAPNGSNIWTVHNAGSSYGVITLEQATYNSVNAVYAQLSLE
                     VGPENIVNVAKSMGVDESPLDPVLSIVLGSSAVSTLEMANAYSNFATDGLHANDYVIS
                     RILDGSGNVIYEHEIEQTQVADPAIFAAAHRALSKVPTGSGTAPNANIGRPQGGKTGT
                     HQSYLDAWYVGYTPEFSTAVWVGYEAQQVPLTDVVIHGQRYSRVFGGSVPAPIWAEFM
                     SYVMQDLPITDFPPEPSNIEKYLIPPPTQVPSVVGLEQSEAESRLRDAKLNADVVEIA
                     SLEPEGMVVNQSVEPGSTVRQGSVITIWVSTGETPVGEMLSLIGMTVDDALEAIREFE
                     LETGVKLSLVQQKTDVTDAAQVDRIVSTNPAPGDRVEGTVQLIVFVGQLAGP"
     gene            2734..3783
                     /locus_tag="AC161X_07190"
     CDS             2734..3783
                     /locus_tag="AC161X_07190"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR04371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sugar O-methyltransferase"
                     /protein_id="HIR4989371.1"
                     /translation="MHLDSDQLDSMVAEIEEAGGVLMPSQFWARFAARNTSTLLEQGL
                     DSFKRSVNYNYFQWVINSPRQPEFASVLKAWARRPSFGVFGARVCVDGIDWNRPKARR
                     QLPEPFRALGHALYVGMLWEVAQRRVPSDVTEHLEESPLGDPIAVSYRGRQISEDLGN
                     SLLEYGTIAEFVPEARMRNATLLEIGAGYGRFADLFVMAQPDSRVVIVDIPPALAISQ
                     AYLTERHPGLTTHRFARGIDPPALAALVSSTRLAFLTPNQFASLPPLGADLVVNVSSL
                     HEMLPEQISAYLGLIDRHASGGFFYTKQWDHWFNEIDGVQTDRAFYPYPPNWRCLIDR
                     TPIAQPAFFEALFAV"
     gene            complement(4290..5510)
                     /locus_tag="AC161X_07195"
     CDS             complement(4290..5510)
                     /locus_tag="AC161X_07195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140327 - flippase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipopolysaccharide biosynthesis protein"
                     /protein_id="HIR4989372.1"
                     /translation="MSRAVIETERRGFRAPGTTFMVAGGLIGSVGAYVFQAYGARALG
                     EHAFAPISLLWTLFFILATVLLVPVEQYVTREVTSGRKAIPADLKAAGAMAAVGSALG
                     AGYVIFTLDRFFEGSWQYVLQIVLLMVGYSLLFVAKGVLAGRRLFSGVGWVLIVETVA
                     RLVAGIVAIQLAGTAESLGWAMVLGGFSVVGMRWWRRDTGRPQAPATRASKFLGGYVG
                     GTASSQVLLGAAPIAVAALGASPALVSVVFVTFTLFRAPLTLIFALQGRILPYLVGLA
                     HDDDRSRLSRIARNVVVGGAVLAVTGGLVGWLVGPQVVGLLFGEAFAPTQAVAMFTAA
                     GMMAASAAQITGQILVAEARTSRLSAAWLGGLLAGVAALLVLGGEPDIRVAVAFLIGE
                     AVALALMAGLAVRR"
     gene            complement(5507..6790)
                     /locus_tag="AC161X_07200"
     CDS             complement(5507..6790)
                     /locus_tag="AC161X_07200"
                     /inference="COORDINATES: protein motif:HMM:NF014453.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HIR4989373.1"
                     /translation="MRSRFLISSSLTDLAAYAVAAIFASQVVYDTWLPWNSLLGNQVL
                     PMVVIQMGAMTLMSFATARMSGHGVPRPSYGRMFAIFFGTTAITALFLVLFREVYYSR
                     MFLILAGMGWLVLATAHRIVRRRRPWTERIGLITGEKQLAEDLVASPHADVVWVLDPT
                     FDGPTELPDRDVTLAVDLRAVLSERVAQYLSSCDVAGYTIRPFTSIYEEHTGRVPLVH
                     LAEGWEISAPLLAVAPWLPGKRVFDVVLTAITAPLWIALGTLVGLYVKVASPGPAIFR
                     QERVGHDGVPFTMFKFRTMILEAEKDGPQFATEDDQRLIRGGRIMRKSRLDEIPQLWN
                     VLKGEMSLVGPRAEQVSFAETFRQQIPFYGHRHLVRPGITGWAQVNYRYADDQADTIE
                     KLTYDLYYIKHMSPVMDISVLWKSVWTVLTGAGAR"
     gene            complement(6822..8249)
                     /locus_tag="AC161X_07205"
     CDS             complement(6822..8249)
                     /locus_tag="AC161X_07205"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002073.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR4989374.1"
                     /translation="MEINITEGGTDLAGATLIAGVMSDREPLAGSEELVAGVDSRLLD
                     LAKFEGNAGQVLVLPHDTAAGLVLLGVGDEATFDSIRTAVGNGIRAVKTARAVTSLCQ
                     VPIDGATRAVVEGAMLGGYEYRDYKTTGEASPVQILDLIDADGAEVSEAVIGCEVTNL
                     ARDWVNTPALDMSPETLAGRIVRAADEAGVTHQVWDLKRITDEKLGALLGVAAGSDRS
                     PSLVILEYRPDGATSHLGLVGKGITFDTGGLSIKTAPFMEEMKDDMSGAAAVAAAIIG
                     VARLGLPIRVTAVMPLTDNAVGGNATRPGDVLRPISGPTIEVLNTDAEGRLILADGLG
                     VIARYEPDLVIDVATLTGAARVALGDRIAAVFGSSSDVANRVLAAASRAGEFFWELPL
                     FKEYRKQLDSDIADIKNITASRYGGAIAAAVFLAEYAGERPWAHLDIAGPARARETAG
                     EQVKGASGFGARTLIEVARAMSESV"
     gene            complement(8262..8951)
                     /locus_tag="AC161X_07210"
     CDS             complement(8262..8951)
                     /locus_tag="AC161X_07210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989375.1"
                     /translation="MPNDLTSLAEPGLILISWGAGLALVSGLVALARIVGPGFTWVAG
                     SVAALVSLGSAFGDDTWWVRFGLIVLALGLIWARNRQLSGIFQVTAGIAFLMQAIQVG
                     GPLPGLSATLALGGVTGEMLLGHWYLVDPRLPRWALKTLAVTAIAGLAFDSLVLAVAG
                     LPPGGATVAYWVLMSTSVVLMGAVFASLRYPAYAGVMAATGLSYLAVLTSLGGVFLGR
                     VLVAGLGPFGN"
     gene            complement(8984..10717)
                     /locus_tag="AC161X_07215"
     CDS             complement(8984..10717)
                     /locus_tag="AC161X_07215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989376.1"
                     /translation="MAQSRSLIRGAAIGVVGGVLVFAAALLIGLVSSGSRPVFLVGAI
                     AGAVFAFIPGGDRRFEAAGVLVIGVGGVVSGLISGWGWLEYSAVLGSVLAVGWLFTGE
                     RLPNDHWAWFRWGGVLLLLLVYVLLPQVIDGGTLGHDEAAYAVKAKSWLDGTPETGWA
                     PHRATGMSVYGYLVLAAGGAEPGLRMIGLVSVLGLAAGVWALGRRLANPRVGALAAVA
                     VVAGPSMLRRATEYLSDLPAATLLVLCMVVVWKEFGDRDRPTYRLLWLLPLSWAAFYL
                     RYQSILSLALIALVMVVLWWRKIRERPGPVLAVAVVGLLGLIPHAVEAMQLTGKPWGI
                     LTFTGEIAERAYVGEGIFDYLSLLIWPLAGFIGPIAVVAALGGVVAYWRDSLMRERFL
                     FLLVPAVLQVLALGLLSHGEARFVFFPLALIVVAGVMAADRWISEGRTAVAPVAWGLA
                     ILLIGSMGLSAGTVRRAVENRALVNLPMELAAIEVRSQAGSETCGVLTTFTPQVTYYS
                     ECSSLPYLAGEDPGTAVALLEGPKRFMVLVEDGRRQPEGDDLDGLVALTAGPPVTIPG
                     ALDSYVYLFAD"
     gene            10779..11729
                     /locus_tag="AC161X_07220"
     CDS             10779..11729
                     /locus_tag="AC161X_07220"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HIR4989377.1"
                     /translation="MPEQPTLDVIIPAARASGTVGAAIDAVRDQDYPYVTSIVVATSD
                     RDTSEVAMAHGASVVPNPSGRTPDALNLALAAGHGEIVARVDAHSLIPKGYLTRAVGT
                     LLTTGADNVGGMQIPVGDRGWARAIAAAMASPFGAGDARHRIGGSAGPAETVYLGVFR
                     RAVLERLGGFDETFDRNQDYELNHRIRQAGGVVWFDPELRVSYRPRSSLAGLASQYFQ
                     YGQWKREFARRYPGSLRWRQLAPPFLVVLTAVSVVAGLWWPAAWAVPLGYLAALVLIG
                     LTKLPSLGGAALAVPAALATMHLSWGLGFLIGPPGRGRTS"
     gene            complement(11674..12759)
                     /locus_tag="AC161X_07225"
     CDS             complement(11674..12759)
                     /locus_tag="AC161X_07225"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4989378.1"
                     /translation="MTEAAILVVTTVHAADDTRIRERLIRSLEGVGSITYATREPGPT
                     DRSGIVWVPIRGGRFSRNLNALRLMLDKKWRLVILHDPETIPAGALTHLMRHRPIVFD
                     VHEDLPAQVVNKEWAPIWARPILRWLAKILYALAQKSLVLTLAEPGYQGLFTGDRHPV
                     FPNYPRTSVYPDPQISGDGSAVYLGDLTRPRGIEDALTACGTIGVPITAVGRVDDDFA
                     KGLQDQARSAEVSLTLTGRLSNPEALRTAGEASVGLSPLRDIANYRNSLPTKTLEYLA
                     MGIPVVATDLPGTREVLSGLEAVWLVPPHDPVAMAQAITEASRAEAKEAAMAQIGYVR
                     EKFQWPETEVRKFYLDLVGRSETPTPS"
     gene            complement(12756..14051)
                     /locus_tag="AC161X_07230"
     CDS             complement(12756..14051)
                     /locus_tag="AC161X_07230"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017926881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016628 - oxidoreductase activity, acting
                     on the CH-CH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HIR4989379.1"
                     /translation="MSDRATFFAEERWTAGVIGLGYVGLPLLLTAMSNGLDAIGFDVS
                     EDKVRQLSTGHSPIDDVSDEALSAAIAMGVRFTSDPEVLGQADAIFICVPSPLGRNRQ
                     PDLSYIEAAADTVTAIARPGQLISLESTTYPGTTEDYLIPAVERAGLTVDEDVWVAFS
                     PERVSPGDDMKTGDIPKVVGGVTPGSGDVAEAAYRRLVPSVHRVSSARAAELSKLLEN
                     TYRAVNIGLINEIAQLSHELDIDIWEVVDAAATKPFGFQAFYPGPGVGGHCIPLDPQY
                     LAWRAREANFATRFIETADQVNTGMPEYTAQRIADLLNSAGKAVLGSRILVVGVAYKP
                     NVSDDRESASVDVAQRLLGRGAQVVVLDPHLTSERIEALGFEPAIPGESLAGFDLAAI
                     LTDHANLPYSVIARDVPLVFDSRGVYRRLGITAPNVEAL"
     gene            complement(14074..>14897)
                     /locus_tag="AC161X_07235"
     CDS             complement(14074..>14897)
                     /locus_tag="AC161X_07235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989380.1"
                     /translation="RSVEDPWSVDPVNPLAHWTILVNTANVATYLGWDKMYTGQLIAG
                     PPGAIVRFNGKDGGVAVTTDLNGTQVRALLNAYGFRADGQPVRVSPYISAVTDPPGFN
                     DIDGNIFEDAIKWMAFEEITMGCNPPTNTLYCPNANVTRGEMAVFISRAMGLPAPKGD
                     HFTDDTGEFYEGAANRLYEAGITQGCGPNRYCGGQNIPREQMAAFLARTLALPNSSID
                     HFIDDENSQFEGAIDKIAAAGITLGCNPPTNNRFCPKDLVTRGQMAAFFKRAWGS"
BASE COUNT         2905 a         4853 c         4542 g         2597 t
ORIGIN      
        1 ctcgtgcagg atctcgacct cgatgtcgct ggaggtgcgc cggctttcat ccatagtcgg
       61 ggtatcctga catctcccaa ggatcgtagg tcgggaactc tcgcgacacc cgagacgtcc
      121 aatcccccca gatggccagg aaagccctca tacatcagat cgaggcgcgg gagcgtaggg
      181 gccgttggtt cggtgccctc gccacgctgc tctccatgtc ccttctcctt gcgggttggg
      241 tcggtttgtt cgcgttccta gggacaaact ctgcctatgg gacctttgag ggcatcaagg
      301 acgattgggt acccgatacg cgctcgatgg agctcacgct ccccgacctg agccgggtga
      361 gcaggatcta cgccgattcg ggagaggtgc tcgccgagct tcacgacggt cgcaactccg
      421 agcccgtgcc ctatgaggaa gtccctgagg tactcgttca cgccatcctc gccgcagagg
      481 actccgagtt cttcgagcac tctggtgtcg acttttccgc catcgtctcc gcagcgatcg
      541 acaacctgat ctacgacacg acaagaggtg ggtcgaccat cacccagcag gtggtgaaga
      601 acgccttcgt cggctcagag gtcagcattc gccgcaagat caatgaggcc ttcgtgtccg
      661 cggagctgga acgtcgcttc cccaaagagc ggatcctcga gtactacatg aactcggttt
      721 acttcggagc cggcgcctat ggggtgcaga cggcagctgt ggagttcttc ggtaaggagt
      781 tgaaccagct ggacgccagc gaggcggcga ccctggcagt tctggtgcgc aacccttcgc
      841 tctacaaccc gcgtcggcga ccggagatca ccctggagag gcgcgatgac gtcatcaggg
      901 agatggagca ggacggttgg ctgaccaccg ccgaggccga ccacgccgtg ctacaaccgc
      961 ttggagtcat cgaagcccct ctccgtcggg gtgaggccga tcacgtggtc gccgaggtca
     1021 agcgccagtt gctcaacagt gacgagttcg ccttcctcgg ggcaactccg gaggaccgca
     1081 agaaggcgat attcgggtgc ccggccgatg atgttgcctg cgacggcggc ggcggcctcc
     1141 aggtcttcac cactatcgac ctcggcttgc agaagaaggc caacgagatc ctcgcctcct
     1201 ggatgccact accgccgtac gagcagaacg tcgccgcctg caaggggatc ctgcccaacg
     1261 agaccgacgc cttccttgcg acttacgccg agacccactc gtgtgttccg accggcgcca
     1321 tgacaatggt ggacaaccac accggcgccg tgaaggtgat ggcctcaggc ctccccttcg
     1381 agttctccca gttcgatctc gcagttcagg gccgacgcaa ccccggatct tccttcaaac
     1441 cttttggcct ggtggccgcc ctggagcagg gttacacgat gggtcacacc tggagtggcg
     1501 agagcccgat cgagatcgag tgccccttcc cctgtgcacc gaacggatcg aacatctgga
     1561 cggttcacaa cgccggatcc tcctacggag tcatcactct cgagcaggcg acctacaact
     1621 cggtcaacgc ggtctacgca caactctcgc tggaagtcgg accggagaac attgtcaacg
     1681 tggccaagag catgggcgtc gacgaatctc cgctcgaccc ggtcctgtcg atcgtgcttg
     1741 gcagctccgc ggttagcacc ctggagatgg ccaacgccta ttccaacttc gcaaccgacg
     1801 gacttcacgc caacgactac gtcatctcga ggatcctcga cggctcgggc aatgtcatct
     1861 atgagcacga aatcgagcag acccaggtgg ctgatcctgc gatcttcgcc gccgcccatc
     1921 gcgccctctc caaggtcccg accggctcgg gaactgcccc caatgccaac atcgggcgtc
     1981 cccagggtgg aaagaccggg acccaccaga gctatctcga cgcctggtat gtcgggtaca
     2041 caccagaatt cagcaccgct gtctgggtcg gctacgaagc ccaacaggtg cccctgaccg
     2101 atgtagtgat ccacggacaa cgctattcga gggtctttgg tggctcggtg cccgctccga
     2161 tctgggcgga gttcatgagc tacgtcatgc aagacctacc gatcactgac ttcccgcctg
     2221 agccgtccaa catcgagaag tacctgatcc cgccgccgac acaggtcccc tctgtcgtgg
     2281 gcctcgagca gtcggaagcc gaatcccgac tgcgcgatgc gaagttgaac gcagacgttg
     2341 tcgagatcgc gtccctggag cccgagggaa tggtcgtcaa tcaaagtgtc gaacccgggt
     2401 caacagttcg tcagggctcg gtcatcacca tttgggtctc cacgggtgag actccggttg
     2461 gcgagatgct ttccttgatt gggatgaccg tcgacgatgc gctcgaggcg atccgggagt
     2521 tcgagttgga gaccggagtc aagctcagcc tggtccagca gaagaccgat gtcaccgatg
     2581 ctgcccaggt cgaccgcatc gtcagcacca acccggcccc cggcgatcgg gttgagggaa
     2641 ctgtgcaact catcgtcttc gtcgggcaac tggccgggcc gtaaggcgcc tacggccccg
     2701 gtagggtcgg agtgatgccg tcgcctgcgc tccgtgcacc tcgactctga ccaactcgac
     2761 tcgatggtgg ccgagatcga ggaggcgggt ggcgttctca tgccatcgca gttctgggcc
     2821 cgattcgcgg cccgcaacac gtcgaccttg ctcgaacaag gcctcgactc cttcaagcgg
     2881 tcggtcaact acaactactt ccagtgggtc atcaacagcc cccggcagcc ggaatttgcc
     2941 tcggtcttga aggcctgggc ccggcgccct tcgtttggag tgttcggcgc ccgggtctgt
     3001 gtagacggta tcgactggaa ccgacctaag gcgcggaggc agcttcccga gcccttccgt
     3061 gctctcggtc acgctctcta tgtgggaatg ttgtgggaag tcgcgcagcg ccgggttccc
     3121 tccgacgtca ccgagcacct cgaggagtct cctctcggcg atccgattgc tgtgagctat
     3181 cgcggtcgtc agatctctga ggacctcggc aacagccttc tcgagtacgg aaccattgct
     3241 gaatttgtgc cggaggcccg gatgcgcaat gcgacgctgc tcgagatcgg cgccggctac
     3301 ggacgattcg ccgacctgtt tgtgatggcc caaccagatt ctcgcgtcgt cattgtggac
     3361 atccctcccg ccttggccat ttcccaggct tacctcacgg aacgccaccc cggcctaacg
     3421 actcatcggt ttgctcgcgg gatcgatcct ccggccctgg cggccctggt aagcagcaca
     3481 aggctcgcct tcctcacccc caaccagttc gcctctctgc ctccgctggg agcagacctg
     3541 gttgtcaatg tctcttcgct gcatgagatg ctcccggagc agatctctgc atacctggga
     3601 ctcatcgacc gacatgcgag cggaggattc ttctacacaa agcaatggga ccactggttc
     3661 aacgaaatcg atggtgtcca gaccgatcgg gcgttctatc catacccgcc gaattggcga
     3721 tgcttgattg accgtacgcc aatcgcccaa cctgcattct tcgaggctct attcgcagtc
     3781 tgaagcggga agggcatcac tcaccagcag ccaacgatat ctttcgcgcc ctcatctacg
     3841 actcttgacg ttagttgacg ttcttctgta gaactcgaca catgagaacc gaagtgcccg
     3901 ggagtggtcg agatgcacgt cctccgcatc ggcaggcctt ggggctctcg ggtgcttcgg
     3961 tccgcccgac agcgcgggtg aggcccgtag tgccggttcg cctcgagtcc tctgccgccc
     4021 gattcgcacc gaccgatccc tatgtccgca ggttctgggt agccgccctc ggatctgccg
     4081 ctgttgccga gctgctccgc ctggtgcgag cggccgaaaa gggggagggg gtgcggctac
     4141 ccagatatct ccccaccctg attcgcaccg gcctcgtcaa agctgacagt ggcggtctcg
     4201 tggtcctgga ccgtttcccc caggtcccca agaatctcct ttggcggttc ccgcccagcc
     4261 tcgctgccga ccacaggcgt tggcttggcc tatctccgaa ccgcgagccc agccatcagg
     4321 gcaagggcga cagcttcgcc aatgaggaag gcgaccgcga cgcgaatgtc cggctctccg
     4381 ccgaggacca gtaaggctgc aactccggcc aacaatcctc caagccatgc ggcgctgagc
     4441 cgtgaggtac gggcttcggc caccaggatt tgacctgtga tctgggcggc agaagcagcc
     4501 atcatcccgg cggccgtgaa catggccaca gcctgtgtcg gggcaaatgc ctcaccgaac
     4561 agaagcccga caacctgtgg cccgacgagc cagccgacca agccaccggt tactgcgagt
     4621 accgctccgc cgacaacgac attccgtgcg atgcgtgaca gtcgacttcg atcatcgtca
     4681 tgcgccagcc cgaccaggta gggaagaatc cggccctgaa gggcgaagat gagtgtgagt
     4741 ggggccctga agagagtgaa agtgacgaag accacagaca ccagtgcagg gctcgcgccg
     4801 agggccgcca ccgcgattgg agccgcgcca agcaggacct gagacgaagc ggtgccaccg
     4861 acgtagccac ccaggaactt ggacgctctc gtcgccggag cttggggccg gccggtgtcg
     4921 cggcgccacc atctcatccc cacgaccgag aagccgccca gcaccatggc ccacccaagc
     4981 gactcggccg ttcccgccag ttggatggcg acgatgccag caaccagcct ggccacagtc
     5041 tccactatca gcacccatcc caccccggag aacagccgcc tccctgcgag gacgcccttg
     5101 gcgacaaaga ggagggaata ccccaccatc agcaggacga tctggaggac gtattgccag
     5161 ctgccctcga agaaccgatc cagggtgaag atcacatatc cggcgccgag cgccgatccc
     5221 acggccgcca tggcaccagc cgctttcaga tctgcaggga ttgccttgcg ccccgacgtc
     5281 acctcccggg tcacgtactg ctcgaccggg accaggagca ccgtcgccag aatgaagaac
     5341 aaggtccaca gcaagctgat cggcgcgaat gcgtgctctc ccagagcgcg ggctccgtac
     5401 gcctggaaca cgtatgcgcc aactgatccg atgaggcctc cggccaccat gaaggtggtg
     5461 ccgggagccc gaaaccccct ccgctcggtt tcgatcaccg cgcgactcaa cgagcgccgg
     5521 ccccggtgag gaccgtccag acgctcttcc agagcacgct gatgtccatc acaggagaca
     5581 tgtgcttgat gtaatagagg tcataggtca acttctcgat ggtgtcagct tgatcatcgg
     5641 catatcggta attgacctga gcccagccgg ttatgccggg acgcaccaga tggcgatgcc
     5701 catagaaggg gatctgctgt cggaaggtct ccgcaaagga aacttgctcg gcccgagggc
     5761 cgacgaggct catctcgcct ttgagcacgt tccacaactg agggatctca tccagtcggg
     5821 acttgcgcat gattcgccct ccccggatca gtcgctgatc gtcctcggtg gcgaactgtg
     5881 gcccgtcttt ctcggcttcg aggatcatcg ttcggaactt gaacatcgtg aacggcactc
     5941 cgtcatggcc gaccctctct tgcctgaaga tggctggccc tggcgaggcc accttcacgt
     6001 agaggcccac cagagtcccg agggcgatcc aaagcggggc agtgatcgcc gtcaggacca
     6061 catcgaagac cctcttcccg ggcagccatg gtgcgaccgc cagtagcggc gctgagatct
     6121 cccagccttc agccaggtga accaagggca cccggccggt gtgctcttcg tagatggaag
     6181 tgaaaggtcg aatcgtgtag ccagcgacgt cacatgacga caggtactgg gcgacccgtt
     6241 ctgagagaac ggccctcaga tcgacagcaa gcgtcacgtc tcgatcgggg agctctgtcg
     6301 ggccatcgaa ggtcggatcg aggacccaaa ccacgtcggc gtgaggtgag gccaccaagt
     6361 cctctgccag ttgcttctct ccggtgatca ggcctatgcg ctcggtccac ggccggcgcc
     6421 gccgcacgat tctgtgggca gtagccagaa ccaaccagcc catgccggcg aggatcagga
     6481 acatccgtga gtagtagacc tctctgaaca gcaccaagaa cagggccgtg atcgcggtgg
     6541 taccgaaaaa gatcgcaaac atccgcccgt aactgggccg gggcacacca tgccccgaca
     6601 tccgggccgt ggcgaacgac atcagggtca tcgcccccat ctggatgacg accatcggca
     6661 gtacctgatt cccgagcagg gaattccaag gaagccaggt gtcgtagacc acctgggagg
     6721 cgaagatcgc cgcaacggcg taagcggcga gatcggtgag actggacgag atcaggaacc
     6781 ggctgcgcac gattccagat gctaggtaac cggtcgggct tctacaccga ctcgctcatc
     6841 gcccgcgcga cctcgatcag ggtccgagcc ccgaatcctg acgctccctt gacctgctcc
     6901 cctgccgtct cccgggccct ggccggacct gcgatgtcga gatgggccca gggccgttcg
     6961 ccggcatact cggcaaggaa gacggccgcc gcgattgctc ccccatagcg actggcggtg
     7021 atgttcttga tgtcggcgat gtcggagtcg agctgctttc ggtactcctt gaacagcggc
     7081 aactcccaaa agaactcccc tgcccttgaa gctgcagcca ggactcgatt ggcgacatcg
     7141 ctgctcgacc cgaagacggc cgcaatgcgg tcgcccaggg caacccgggc cgcgccggtc
     7201 agagtggcga cgtcgatcac gaggtccggc tcatagcgtg caatgactcc aaggccgtca
     7261 gcaaggatga gacgaccctc ggcatcggtg ttcaaaacct cgatggtcgg ccccgagatc
     7321 ggtcgcagta catcgccggg acgggtggca tttcccccga cggcattgtc ggtcagcggc
     7381 atcaccgcag tcacccggat cggcaggcca agacgagcga ctccaatgat cgcagccgcc
     7441 actgcagcgg caccggacat gtcgtccttc atctcctcca tgaacggtgc ggtcttgatc
     7501 gacaggccac cggtgtcgaa ggtgatcccc ttgccgacca gcccgagatg tgaggtagcg
     7561 ccgtccggcc ggtactcgag gatcacgaga cttgggcttc gatccgatcc ggcggccacg
     7621 cccagtaggg cgccgagctt ctcgtcggtg atgcgcttca ggtcccatac ctggtgggtt
     7681 acgcccgcct catccgcggc acgaactatc cgaccggcaa gagtctccgg cgacatgtcc
     7741 aaggccggag tgttgaccca atcccgggcc aggttggtca cttcgcaacc gatcacggct
     7801 tcggagactt ctgctccatc ggcatcgatc aagtcgagga tctggaccgg tgaggcctcg
     7861 cccgtcgtct tgtaatcgcg gtactcatag ccaccgagca tggctccttc gacgactgcc
     7921 ctcgttgcgc catcgatggg tacctgacac agtgaggtca cggcgcgggc ggtcttgacg
     7981 gcacggatgc cgttccccac cgcagtgcga atcgaatcga aagttgcctc gtcgccaacc
     8041 ccaagcaaga ccagcccggc ggcggtgtca tggggcagga ccaggacctg accagcgttg
     8101 ccctcgaatt tcgcaagatc cagaagtcgg gagtcgacac cggcgacgag ctcctcgctg
     8161 cccgccagcg gctcacggtc cgacatgacg cctgcgatga gggtcgcccc tgccaggtcc
     8221 gtgcctccct ctgtgatgtt gatctccaag tccactcctt ttcagtttcc aaacggtccc
     8281 aggccggcca ccaagacccg acccagaaag acgcctccca ggctggtgag aacagccaaa
     8341 tatgacaaac cggtagctgc catgacgccg gcataggccg gatagcgcag cgaggcgaac
     8401 accgcgccca taaggactac cgaagttgac atcagcaccc aataggcgac tgtcgcccca
     8461 cccggtggga gcccggccac ggctagcacc aggctgtcga aagccagtcc ggcgatcgca
     8521 gtcaccgcca gagttttgag cgcccacctc ggaagccgag gatcgaccaa gtaccagtgg
     8581 ccgaggagca tttcaccggt caccccaccc agggccagag tggcggagag gccgggcagc
     8641 gggccgccga cctgtatcgc ctgcatcagg aacgcgatcc ccgcggtcac ctggaagatc
     8701 cctgatagct ggcggttccg tgcccagatc agaccaaggg cgagaacgat gagaccgaat
     8761 ctgacccacc aggtgtcgtc tccgaaagct gagccaagac tgaccagcgc cgccaccgat
     8821 ccggcgaccc aggtgaaacc gggcccgacg attcgggcga gggccaccag gccggagacc
     8881 aaagcaagcc ctgcccccca cgagatcagg atcagccctg gctcggcaag gctcgttagg
     8941 tcattgggca cagcgacgga tcatatgggc gaacccgttg gatctagtcg gcgaacaaat
     9001 acacgtagga atccagggca cccgggatcg tcaccggtgg cccggcggtc aatgcaacga
     9061 gcccgtccag gtcatccccc tccggttgtc gcctgccatc ttccacgaga accatgaatc
     9121 gcttcgggcc ttcgaggagg gcgacggccg tccccggatc ttctcccgcc aagtacggaa
     9181 gactcgagca ctccgagtag taggtcactt gtggggtgaa agtggtgagt acaccacagg
     9241 tttcagatcc tgcctgagag cgaacttcaa tggccgcaag ttccatggga agattgacca
     9301 gagcccggtt ttccacggcg cgtctcactg ttccggccga caaccccatg gatccgatca
     9361 gaaggatggc cagcccccat gccaccgggg ccaccgctgt cctcccttcg gaaatccatc
     9421 ggtctgccgc catcaccccg gcgaccacga tgagggccag cggaaagaac acaaatcgcg
     9481 cctctccgtg agacaagagc ccgagggcca acacctgcag caccgccggc accagaagaa
     9541 aaaggaaccg ctctcgcatc agggaatcgc gccagtaggc gactacgccg cccagcgccg
     9601 cgaccacagc gattggaccg atgaagccgg cgagaggcca gatcagaagc gacagatagt
     9661 cgaatattcc ctcaccgacg taggcgcgtt cggcaatctc accggtgaag gtgagaatcc
     9721 cccaaggctt cccggtcaac tgcattgctt ccaccgcgtg tgggatgagg ccgagaaggc
     9781 cgacgacagc aactgccaga accggacctg gtcgctccct gatcttcctc caccagagca
     9841 cgaccattac caaggcgatc agcgcgaggc tgagaatcga ttgataacgc aggtagaagg
     9901 cagcccatga caaggggagg agccacaaca agcgataggt cggacggtca cgatcgccga
     9961 actccttcca cacgacgacc atgcaaagca cgaggagggt ggctgccggg aggtcactga
    10021 ggtactcggt ggcccgtcgc agcatggagg gcccggccac gacggctacg gcagccagcg
    10081 cgccgactcg gggattggcc agccgccgcc ccagggccca gacccctgcg gcaagcccga
    10141 gtacggacac cagaccgatc attcgaagac cgggttcggc accacccgct gccaggacca
    10201 agtagccgta gaccgacatc ccggtagctc gatggggcgc ccatccggtc tcgggggtgc
    10261 catccaacca tgacttggcc ttgacggcat aagcggcctc gtcatgcccc aaggttccgc
    10321 catctatcac ctgcggcagt aacacataga caagcagaag cagcaagacc ccgccccacc
    10381 tgaaccatgc ccagtgatcg ttcggtaagc gctcaccagt gaacagccag ccaaccgcaa
    10441 gcaccgatcc aaggacagcc gagtactcca gccatcccca gccagagatc agacccgaaa
    10501 cgacaccgcc cactccgatc actaagaccc cggcggcttc gaaccggcga tcgccacccg
    10561 gaataaaggc gaagacggct ccggcgatcg cccccacgag gaagacgggg cgagagcctg
    10621 atgagaccaa accgatcagc agagcggctg cgaacacgag aacgccgccc acgacgccga
    10681 tggccgcgcc tcggatcaat gatcggctct gggccatggg tggcacctta cgggtgcatt
    10741 gatggcgagg acaccgcctg ccctgggaag atgcaggggt gccggagcaa ccaacgctcg
    10801 atgtcatcat cccggcggcc agggcctcgg ggaccgtggg cgcagctatc gacgccgtcc
    10861 gagatcagga ctatccctat gtcacctcga tcgtggtcgc gacctcggac cgtgacacca
    10921 gtgaagtggc gatggcacac ggcgcctccg tcgtccccaa cccgagtgga cgaacgccag
    10981 acgcactcaa tctcgcccta gccgcaggtc atggggagat cgtggcccgt gtggacgctc
    11041 attccctgat tccgaaggga tacctcaccc gcgccgtggg caccctgctg accaccggag
    11101 ccgacaatgt cggcggcatg cagattccgg tgggagatcg tggctgggcc agagcgattg
    11161 ctgccgccat ggcatcgcct ttcggcgccg gtgatgccag gcaccggatc ggagggtccg
    11221 cgggcccggc cgaaacggtc tatctcggtg tctttcgacg tgccgtcctc gagcgcctcg
    11281 gcgggttcga cgaaaccttc gaccggaacc aggactatga actgaatcac cggatacgtc
    11341 aggcgggtgg ggtggtgtgg ttcgacccgg aacttcgcgt gtcctatcgg ccccgaagct
    11401 cactcgccgg cctggcgagc cagtactttc agtacgggca gtggaaaagg gagttcgccc
    11461 gccgatatcc gggctccttg agatggcgac agctggctcc gcctttcctg gttgtcctca
    11521 cggcggtctc tgtcgtcgcc gggctctggt ggccggccgc ctgggcagtg ccactcggct
    11581 acctcgccgc cctcgtgctg atcggtctga ccaaactccc aagcctcggt ggcgcggcgc
    11641 tcgccgtgcc cgctgccctg gcaacgatgc acctcagctg ggggttgggg tttctgatcg
    11701 gcccaccagg tcgaggtaga acttcctgac ctcggtttcg ggccactgga atttctcccg
    11761 cacgtagcca atctgcgcca tcgccgcttc cttcgcttcg gcgcgagagg cctcggtgat
    11821 ggcctgcgcc atcgccacag gatcgtgcgg cgggaccagc cataccgcct cgagcccgct
    11881 caacacctcg cgggtaccgg ggagatcagt ggccaccacc ggaatcccca ttgccaggta
    11941 ctcgagagtc ttggtgggca gggagttccg atagttggcg atgtcccgca aaggcgacag
    12001 gccgactgag gcctcgccag ctgtgcgcag cgcttcgggg ttcgagagcc gaccggtgag
    12061 ggtcagggat acctcggcgg atcgggcttg atcttgcaac cccttcgcaa agtcgtcatc
    12121 gaccctgccg accgcggtta tcgggacgcc aatggtcccg caagcagtca gagcgtcctc
    12181 aatcccacgt ggtcgggtca ggtcaccgag atagaccgcc gatccgtctc ccgagatctg
    12241 cggatcgggg taaacgctgg tccgagggta gttggggaac acagggtggc ggtctccggt
    12301 aaacagcccc tggtagcccg gttcggccag agtgagcacc agcgacttct gcgccaatgc
    12361 gtacaggatc ttcgcaagcc aacgaagaat tggtctcgcc cagatcggag cccactcctt
    12421 gttcaccacc tgggcgggca gatcctcatg aacgtcgaag acgatcggcc gatgacgcat
    12481 caagtgggta agcgctcctg cgggaatggt ctcgggatcg tggaggatca ccaatcgcca
    12541 tttcttgtcg agcatgagcc tgagcgcgtt gagattgcga ctgaaccggc caccgcgaat
    12601 cgggacccag acgatgccag accggtctgt agggccaggc tcacgtgtgg cgtaggtgat
    12661 cgaaccgacg ccctcgaggc tgcggatcaa tcgctccctg attcgggtgt cgtctgctgc
    12721 gtgaactgtg gtgacgacga ggatcgccgc ctccgtcata gagcctccac attcggtgcc
    12781 gtgatcccga gacggcgata cacgcccctg gagtcgaaga ccagagggac gtcacgggcg
    12841 atcaccgagt agggcaagtt ggcgtgatcg gtaaggatcg cggcgagatc gaatccggcg
    12901 agactctcgc ccggaatcgc gggctcgaat cccagcgctt cgatgcgctc cgaagtgagg
    12961 tgggggtcga gcaccaccac ttgtgctcct cgcccgagga gacgttgcgc cacatcgact
    13021 gacgcagact cgcggtcgtc ggagacgttg ggtttgtagg cgacgcccac caccaggatc
    13081 cggctgccaa gcacggcctt gcccgccgag ttgagtaggt cggcgatccg ttgcgccgtg
    13141 tactcgggca tccccgtgtt gacctgatcg gcagtctcga tgaagcgggt cgcgaagttg
    13201 gcctcccttg ctcgccaggc gaggtattgc ggatcgagag gtatgcaatg ccccccaacg
    13261 ccgggcccgg gatagaacgc ctgaaagccg aacggcttgg tcgccgcggc gtctacgacc
    13321 tcccagatgt cgatgtcgag ctcgtggctg agttgggcga tctcgttgat gagcccgatg
    13381 ttgactgcgc ggtatgtgtt ctcgagaagc ttggacaact cggcggctcg ggcggagctg
    13441 actcggtgca cggatgggac cagtcttcgg tacgcagcct cggcgacatc gcctgaccct
    13501 ggtgtgaccc cacccaccac ctttggaatg tctcccgtct tcatgtcatc gccgggtgag
    13561 acccgttccg gtgagaaggc aacccagacg tcctcatcga cggtgagacc cgccctctcc
    13621 accgcgggga tcaggtagtc ctcggtggtg ccgggatagg tagtcgactc cagcgaaatc
    13681 aattgtccgg gtcgtgcgat ggccgtcacg gtgtcagcgg ccgcctcgat gtaggagagg
    13741 tccggctggc ggtttcgccc gagcgggctg ggaacgcaaa tgaagatggc atcggcttgg
    13801 ccgagaacct cggggtccga agtgaatctg acccccatgg cgatcgcagc cgaaagtgcc
    13861 tcgtcggaga catcatcgat cggggaatga cctgtcgaca gttggcgaac cttgtcctcg
    13921 gagacgtcga aaccgatcgc atcgaggcca ttgctcattg cagtcaagag caggggcagg
    13981 cccacgtatc caagaccgat gacccccgca gtccaccgct cctcggcgaa gaaagttgcc
    14041 cgatcgctca ctgccgagga atggtagggc cggtcacgac ccccaggccc gcttgaagaa
    14101 agcagccatc tgccccctgg tcaccaggtc cttgggacag aatctgttgt tggtaggcgg
    14161 gttgcacccc aacgtgatcc cggcggcagc gatcttgtct attgccccct cgaactggga
    14221 gttctcgtcg tcgatgaagt gatcgatgct cgagttgggc aaggcgaggg ttcgggccag
    14281 gaacgcggcc atctgctccc ggggaatgtt ctgacctccg cagtagcggt tggggccaca
    14341 accctgggtg atgcctgcct catagaggcg gttggcagcc ccctcgtaga actcgccagt
    14401 gtcatcggtg aagtggtcgc ccttcggagc cggcaagccc atcgcccgtg agatgaacac
    14461 cgccatctcg cccctggtca cgttggcatt cgggcaatag agggtgttgg tcggcgggtt
    14521 gcaccccatc gtgatctcct cgaaggccat ccacttgatg gcgtcttcga agatgttgcc
    14581 gtcgatgtcg ttgaatcctg gagggtcggt gaccgcgctg atgtatggcg aaacccgcac
    14641 cggctggccg tcggcccgga acccataggc gttgagcagg gcgcggactt gggtgccgtt
    14701 gaggtcagtg gtgactgcca ctccgccgtc cttgccattg aagcggacga tcgctcccgg
    14761 gggcccggct atgagctgac cggtgtacat cttgtcccag ccgaggtagg tggccacgtt
    14821 tgcggtgttg accaggatcg tccaatgcgc cagcgggttc accgggtcca ccgaccaggg
    14881 atcctccacc gagcgca
//
LOCUS       DBNHXU010000283         2182 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2475770, whole genome shotgun sequence.
ACCESSION   DBNHXU010000283 DBNHXU010000000
VERSION     DBNHXU010000283.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2182)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2182
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2475770"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..527
                     /locus_tag="AC161X_07240"
     CDS             <1..527
                     /locus_tag="AC161X_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989381.1"
                     /translation="TRPRAVGVVLLMLLFASCSSGTADVVLQATPGRPDNAEATRIEA
                     DLATASDLVFLGTVERPESYVTLVTYTNEIGESCVAFYSTDGHASSGCGDVDGVNDPV
                     GVLQSHDDRVIGVVARTSPDITEVRLATTNGLTSSVRPLGDFSYIEVDDDGSGYTLSL
                     LAGDDVVYNERSGP"
     gene            566..946
                     /locus_tag="AC161X_07245"
     CDS             566..946
                     /locus_tag="AC161X_07245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4989382.1"
                     /translation="MAISLDHTIVPSHDKVEAARFIARIFDLPFTDAGGYFAPVRVNT
                     ELTLDFASAEDVIELHYAFLVGEEEFDAIFGRIQADGVGFGSGPSSPEDGKINTRRGG
                     RGLYFSGGPDPHLWEIMTRAETGT"
     gene            complement(950..1342)
                     /locus_tag="AC161X_07250"
     CDS             complement(950..1342)
                     /locus_tag="AC161X_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989383.1"
                     /translation="MDDYILFDIPEGSDPPDRKFLEQLGHRVMVCAGPAPGTVCPILR
                     GEGCELAEGAQGIVFELDLDSAQHQAILAKYKEFLRGDVPIRVVLRPGQAEKYPSLVK
                     GLKVLTHAPVAGDLDALEAEVAASRQEQ"
     gene            complement(1503..>2182)
                     /locus_tag="AC161X_07255"
     CDS             complement(1503..>2182)
                     /locus_tag="AC161X_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989384.1"
                     /translation="GGSLPDISLPEVTTPDLTLPEVTTPDSTEPEATTPEETAPEVTE
                     PEVTEPEATTPNSTEPSEAAAPPEEGETSPWLWIVLAGLVIALLVGALAARGRARSAE
                     ASWKERARSALLGVAAIEATLGRPATLDPSDHEAMRRSVASARDQSVSFEQLAASASD
                     QALAQQAAAVSDALRVLATSSEIEVEGLASGALNTVANREAAESDRRVHLQTLRQAAD
                     RLSQKVQ"
BASE COUNT          395 a          680 c          679 g          428 t
ORIGIN      
        1 tgaccagacc ccgcgcagtg ggcgtcgtcc tgttgatgct cctctttgca tcctgcagta
       61 gtggcacagc cgatgtcgtg ttgcaggcga ctccaggtcg gcccgacaat gccgaggcca
      121 cgagaatcga agcggacttg gccacagcga gtgacctcgt gtttctgggc actgttgagc
      181 gtccagaaag ctatgtgacc ctcgtcacct acaccaacga gatcggtgag agctgtgttg
      241 ccttctattc cactgacggg cacgcctcct cagggtgcgg tgacgtcgac ggtgtgaacg
      301 atccggtggg cgtattgcaa agccacgacg atcgcgtcat cggagtggtg gcccggacct
      361 cgcctgacat cacggaagtc agattggcga caacaaacgg tcttacttcc tccgttcggc
      421 cccttggcga tttcagttac atcgaggtcg atgatgacgg ctccggctac accttgtcac
      481 tcctggcggg cgacgacgtc gtctacaacg agcgatcggg tccctgaatt gtcctggtga
      541 gccgcgagct ttgaatggag gttccttggc tatctcactc gaccacacga tcgtcccctc
      601 ccacgacaag gtggaggcgg cgcgattcat tgcccggata ttcgatctgc cgtttacgga
      661 cgctggcggc tacttcgccc cggtcagggt caacaccgag ctcacgctcg acttcgcctc
      721 tgccgaggac gtgattgagc tccattacgc cttcttggta ggtgaagagg agttcgacgc
      781 catatttggc cgaattcagg ccgatggggt cggcttcggc agtggtccga gctcaccgga
      841 ggatgggaag atcaacactc gacgaggcgg gcgagggctc tacttctcag gcggaccaga
      901 tccgcatctc tgggagatca tgacccgagc cgagaccggc acctgacatt cattgctcct
      961 ggcgagaggc tgccacctcg gcttccaggg cgtcgagatc gcccgctacc ggagcgtggg
     1021 tcagcacctt cagacctttg accagactcg gatacttctc ggcttgtcca gggcgcagta
     1081 ccacccggat cggcacgtca ccacggagga actccttgta cttggcgagg atggcttgat
     1141 gctgggcgct atcgagatcc agctcaaaca cgattccctg ggccccttca gctaattcgc
     1201 atccctcacc cctcaggatg ggacacacgg ttccgggggc gggtcccgca cagaccatca
     1261 cccggtggcc caattgctcg agaaacttcc gatcgggggg atccgaccct tccggaatgt
     1321 cgaagaggat gtagtcgtcc atctcgtcct ccgttcccct cgagcattgg ttgaaagcgc
     1381 cagcgcctgc caggggagaa aggcccttcg tggacacacc acttcctgag cctctcggaa
     1441 ccgtgattca acgagccagg tcgatgctca acatggagaa tggaatggag actgctgaac
     1501 cgctattgga ccttctggga cagccggtcc gcagcctggc gcaatgtctg gagatggacg
     1561 cgtcggtccg actcggccgc ctcccggttg gcgactgtgt tcagcgctcc cgaagcaagg
     1621 ccttcgactt caatctcact ggatgtggcc aggacgcgga gggcgtcgga tacggctgca
     1681 gcttgttggg caagagcctg atccgacgca ctggcagcca gctgttcgaa ggaaaccgac
     1741 tgatctcggg cactagcgac actccgacgc atggcctcgt ggtccgaggg gtcaagagtc
     1801 gcaggtcgcc ccaaagtcgc ctcgatagcg gcaacgccca agagggcaga gcgggcccgt
     1861 tccttccagg aggcttcggc agagcgggct cgacctcgcg ctgccagcgc cccaaccagg
     1921 agtgcgatga ccagcccggc caggacgatc cacaaccacg gggaggtctc accttcctcg
     1981 ggaggcgccg ccgcctcgct cggttcagtc gagttcggtg tcgtcgcctc cggctcggtt
     2041 acctcaggct cggtcacttc cggcgcggtc tcctcgggtg tcgtcgcctc cggctcagtg
     2101 ctgtcggggg tggtgacttc gggcagggtc aagtcggggg tggtgacctc aggaaggctg
     2161 atgtcgggca agctgccgcc cg
//
LOCUS       DBNHXU010000284         3131 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4658159, whole genome shotgun sequence.
ACCESSION   DBNHXU010000284 DBNHXU010000000
VERSION     DBNHXU010000284.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3131)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3131)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3131
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4658159"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC161X_07260"
     CDS             complement(<1..629)
                     /locus_tag="AC161X_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989385.1"
                     /translation="MIDVGTDEPRAGTSKRPRVWVAAGLAAVGALALGIALRIGDGEG
                     SPVSVANAFMEARDNLDADTIEALFAPDVPISDGFIETIDQYRLYFDWLRASNWRWDI
                     GTCAEASTGPGGTLVRCDYVSENDWTRALSHSPISGFIEILVADDEITGLVHTGEIAQ
                     FDEIWDTVTDWIAANHPDTVDQVLTPDLRRPVLDANSVALWEQYTEEYVA"
     gene            747..2153
                     /locus_tag="AC161X_07265"
     CDS             747..2153
                     /locus_tag="AC161X_07265"
                     /inference="COORDINATES: protein motif:HMM:NF012464.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HIR4989386.1"
                     /translation="MKPTQATKSGHIRAAVYTRISKDQSNGLGVARQETLCRKLAEEK
                     GWEVAAVYTDNDISAYNGAPRPAYARMLEDLQAGTVNAVICVDLDRLTRRPSEIEEFI
                     TLADQTGAHLANVSGDLDLSSSDGRFRARIMGAVARQESEKKSERIIRANDQRRARGA
                     PHLGGVRPYGYLPARATDGTPTFRIVEDEATRIRNAIDDHLAGKSLNQIAREWNQAGT
                     RTTKGGVFSATTVRRVLASPVCAGLLKLDRETMAGNWPAIIDRATHEAIRQKTEARKG
                     QGQGGGRPPKKLLTGLVRCANCGAPLYWRVSIRREGAYVHVAKPETGPCVTPGLSINA
                     ESLEAQVAAGVLSRLDTPEVRSEMNRPLADPTAAFLEQSEAALSTLTHDRYVRGIVSE
                     RNYLEAAEALRVKIATLQMELAAPLPELPKGDPATLWDGATLEERHRIVGTMVSGITV
                     SEGKSGDYDPERISITWR"
     gene            complement(2405..>3131)
                     /locus_tag="AC161X_07270"
     CDS             complement(2405..>3131)
                     /locus_tag="AC161X_07270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989387.1"
                     /translation="GEGNGWVYDVTDEGYGGLMSVRSTTESKPLVQLSADVCLVCGSR
                     LRPAVVYDGDGEYLPAPVTWRRPKFLGVRGRTERDCDEALDRSDPHKALWHFEVRVEK
                     LVSTMNPEAHRLCVEVIEKSHADVEGKHPSAKTRIDRAELKMRGQSEFHTWRQASWAW
                     HVLTYGMDEGWDRSFGWITEGYVFPAVTRPLEQRYIDLALLPRYPDGSPYAGPEDPKK
                     HGAWIEKHGAFAYRTLEEARLIV"
BASE COUNT          573 a         1040 c         1000 g          518 t
ORIGIN      
        1 gcgacatact cctcggtgta ctgctcccaa agtgcgaccg agttggcgtc gaggacgggc
       61 ctccgcaggt cgggggtgag gacctgatcg accgtgtccg gatggttggc tgcaatccag
      121 tcggtcaccg tgtcccaaat ttcatcgaat tgggcgatct caccggtgtg gaccaaaccc
      181 gttatttcat cgtccgccac caggatctca atgaagcctg agatcggaga atgcgacagc
      241 gctcgcgtcc agtcgttctc actcacgtag tcacaacgga ccagggtgcc gccaggcccg
      301 gtggaggctt cggcgcaggt gccgatgtcc catctccagt tggaggcgcg aagccagtcg
      361 aagtagagcc ggtactggtc gatcgtctcg atgaagccat cgctgatcgg cacgtcgggg
      421 gcgaacagcg cctcgatggt gtcggcgtcg aggttgtccc tggcttccat gaaagcgttg
      481 gccaccgaaa cggggctccc ctccccatcg ccgatccgaa gggcgatccc cagcgccagc
      541 gctccgaccg cggccaggcc tgcggcgacc cagacccgcg gcctcttgct agtgccggcc
      601 ctcggctcgt cggttccgac gtcgatcatc tcgaaacgtt agggccgatt caaggggcgg
      661 agcacacccc ttctatcggc gcccggccct ccgtacggtc gactccatgt acgcggccac
      721 cacgtcggtt gagtaggata agttttatga aacctactca agcaactaag tcaggccata
      781 tccgggcagc cgtctacaca aggatctcga aggaccagag caacggtctc ggggtcgccc
      841 gccaggagac tctgtgccgc aagctggccg aggagaaggg ctgggaggtg gcggcggtgt
      901 ataccgacaa cgacatctcg gcctacaacg gcgcaccgcg ccccgcttac gcccggatgc
      961 tcgaggacct ccaggccggg accgtgaatg ccgtgatatg cgtcgacctc gaccggctca
     1021 cccgccgtcc ctccgagatc gaggagttca tcacccttgc cgatcagacc ggggcccacc
     1081 tggccaacgt ctcgggagac ctagacctca gctcgtcaga tggcaggttt cgggcacgga
     1141 tcatgggagc cgtcgcgcgc caagagtccg agaagaagtc ggagcggatc atccgagcca
     1201 acgaccagcg gagggcaagg ggagcccctc atcttggagg ggtccgtccg tatggatacc
     1261 tcccggcccg cgcgaccgat ggcacgccca ccttcagaat cgtcgaggac gaggccacgc
     1321 gcatccgcaa cgcgatcgat gaccacctcg ccgggaagag cctgaaccag atcgcccggg
     1381 agtggaacca ggccgggacc agaaccacca agggcggcgt gttctctgcg accacggtgc
     1441 gccgggtgct ggcgagtccg gtctgcgctg gtctgctcaa gctcgacaga gagacgatgg
     1501 cggggaactg gccggcgatc atcgaccggg ccacccatga ggcgatccgc cagaagaccg
     1561 aagcccgaaa gggccagggc cagggtggag gccgaccgcc caagaaactc ctcaccggcc
     1621 tggtgcgctg tgccaattgc ggtgctcccc tctactggcg agtcagcatc cgacgggagg
     1681 gggcttatgt ccacgtcgcc aaacccgaga ccggaccctg tgtcactccc ggtctgtcga
     1741 tcaatgccga gagcctggag gcccaggtcg cggctggagt gctctcacgc ctcgacacac
     1801 ccgaggtccg ctcagagatg aaccgaccac tggccgatcc caccgcggcc ttcctggagc
     1861 aatctgaggc cgcactgtcc accctcaccc acgaccgata cgtgcgcggg atcgtctcag
     1921 agcgcaacta cctggaagcc gccgaagctc tccgggtgaa gatcgccact ctccagatgg
     1981 agctcgccgc accgctgccc gagttgccga agggagaccc ggccactttg tgggatggag
     2041 ccacactcga ggagcgtcac cggatcgtgg gaacaatggt ctctgggatc acggtgagcg
     2101 aggggaagtc cggggattac gacccggagc ggatctcgat cacctggcgg tagctgctct
     2161 ggctgacgag tcctcgctac ccctggcggg tccgaccagg gctggtacag aagtcgagga
     2221 cgtacctcat ggatcggctc agaccaaaag ggcgagaggg gcgggagttg agcaaccgcc
     2281 cctctctgtc gggactcggg ttgaaccctg cgcggtattc cccccgatgc gcagcagcac
     2341 gctgtgcgct ttccgtcgag gatcaacacc cgtgatgtcc gggtcatcga cggcgtccca
     2401 atgctcaaac gatcagccgc gcctcctcca gcgtccgata ggcgaatgcg ccgtgcttct
     2461 cgatccaggc gccgtgcttc ttgggatcct cggggcctgc gtatgggctg ccgtccggat
     2521 accgggggag gagcgccagg tcgatgtacc gctgctccag gggacgagtg accgcgggga
     2581 acacgtagcc ctcggtgatc cacccgaagc tccgatccca tccctcgtcc atcccgtagg
     2641 tgaggacgtg ccaggcccag gaggcctgcc tccaggtgtg gaactcggac tggccccgca
     2701 tcttgagctc ggcccgatcg atgcgtgtct tggccgaggg gtgcttcccc tcgacgtcgg
     2761 cgtgcgactt ctctatgacc tcgacacaga ggcgatgggc ctcggggttc atggtgctca
     2821 ccagcttctc gaccctgacc tcgaagtgcc acagcgcctt gtgcgggtcg gaccgatcca
     2881 gggcctcgtc gcagtccctc tctgtgcggc ccctgacccc gaggaacttg ggtcgacgcc
     2941 aggtgaccgg ggccgggagg tactcgccat ccccgtcgta gaccaccgcg gggcggagcc
     3001 tcgacccaca gaccaggcag acgtcagcgg agagctggac gagcggcttg ctctcggtgg
     3061 ttgatcgcac cgacatgagg ccaccataac cctcgtctgt gacgtcgtaa acccagccgt
     3121 ttccctctcc g
//
LOCUS       DBNHXU010000285         2081 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3467462, whole genome shotgun sequence.
ACCESSION   DBNHXU010000285 DBNHXU010000000
VERSION     DBNHXU010000285.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2081)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2081
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3467462"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..530
                     /locus_tag="AC161X_07275"
     CDS             <1..530
                     /locus_tag="AC161X_07275"
                     /inference="COORDINATES: protein motif:HMM:NF015963.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0003885 - D-arabinono-1,4-lactone oxidase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="D-arabinono-1,4-lactone oxidase"
                     /protein_id="HIR4989388.1"
                     /translation="AGADFCVVKRYREVEIGTPHEGLPPNERIDRSYRIYPMIYDPNF
                     HEVEYFLPIEQAREIMDEMRKLMLQWLPLSVYPLEIRVVAGDDAWLSPNYQRDNLVVS
                     ISGEPGTEYWPYLRACDRLFAEYGGRPHWGKIHFMTPERVAELFPRYEDFKALRTRFD
                     PKGTFLNPHLRGLFG"
     gene            complement(519..863)
                     /locus_tag="AC161X_07280"
     CDS             complement(519..863)
                     /locus_tag="AC161X_07280"
                     /inference="COORDINATES: protein motif:HMM:NF016001.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR4989389.1"
                     /translation="MRLTTTGRRTGTERTVILGYLEDGPDVVTMAMNGWADGEPAWWL
                     NLQANPEATISLPDGQRTVKGRAAEGEERSRLWAKWRELDEKLDAYAALRSSETAVVI
                     LEPTKPGGPLSE"
     gene            complement(1030..2001)
                     /locus_tag="AC161X_07285"
     CDS             complement(1030..2001)
                     /locus_tag="AC161X_07285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020464974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HIR4989390.1"
                     /translation="MKAIVRDEYGSPGVLRFEDVAKPVPGDGEVLVRVHAASLNTADL
                     DLLRGRPVMTRIGTGLLRPKHRMLGLDIAGRVEATGKNVTSLQPGDEVWADMFSYGHG
                     AFSEFVCASKSAFSTKPAGLTFEEAATVPHSAVLALQGLRGKGEIQPGQRVLIIGAGG
                     CVGPFAIQIAKASGAEVTGVDHTEKLDLMRSAGADHVIDYTREDFTTNGAKYDLILDI
                     VDQHSVLHYRRSLSPRGRYVLVARNLSGFFQAFFLGGWISLTGSKRMGIFMWSPNKKQ
                     DLVFLKDLLERGLIKPLIDRTFELSEVGEALRYLEDGQARGKVTIQL"
BASE COUNT          400 a          688 c          589 g          404 t
ORIGIN      
        1 acgccggcgc cgacttctgc gtggtcaagc gctaccggga agtcgagatc ggcacacccc
       61 acgagggctt gccgccaaac gagcggatcg accgctcgta tcggatctac ccgatgatct
      121 acgacccgaa cttccacgag gtggagtact tcctgccaat cgagcaggcc cgggagatca
      181 tggatgagat gcgcaagctg atgctccagt ggctcccctt gagtgtctac cccctcgaga
      241 tccgggtcgt tgccggcgat gacgcctggc tgagccccaa ctaccagcgt gacaaccttg
      301 tggtgtcgat ctcaggcgag ccgggcaccg agtattggcc gtaccttcgg gcctgtgacc
      361 gccttttcgc cgagtacggc gggagaccgc attggggaaa gatccacttc atgacccccg
      421 aacgagtcgc cgaactgttc cctcgctatg aggacttcaa ggcattgcgc acacggttcg
      481 accccaaagg aacattcctc aatccacacc tgcgcgggct attcggatag cggccccccg
      541 ggcttggttg gctccaggat tacgacggca gtctcggagg atcgaagcgc cgcataggcg
      601 tccaacttct catccaattc gcgccatttc gcccacagac gcgaccgttc ctctccctcc
      661 gcggcacggc ccttcactgt gcgctggcca tcggggaggg agatagttgc ctcgggattc
      721 gcctggagat tcagccacca cgcgggctca ccgtcagccc atccgttcat cgccatggtg
      781 acgacgtccg gaccgtcctc gaggtaacca aggatgacgg tgcgctcggt accggtcctg
      841 cgacctgtgg tcgtgaggcg catcgcgccc caccggttcg cccttcgttc agctcgcgga
      901 cgccataacc cgacgcgacc gcccgtcact cggtagatac cgcggtgggc aaaccaggcg
      961 aggcggacaa accatctcgg agggagccgc acttcttgcc tttccaggtc cgtcatcgat
     1021 gtctccttct cacaattgga tggtgacctt tcctcgcgcc tgtccgtctt ccagatagcg
     1081 cagcgcttcg ccgacttcgc tcaactcgaa ggttcggtcg atcaagggtt tgatcaggcc
     1141 gcgttccagt aggtccttga ggaacaccag atcctgtttc ttgttcgggc tccacatgaa
     1201 gatgcccatc ctcttgctcc cggtcaacga gatccaccct cccaggaaga aggcctggaa
     1261 aaacccactc agattccgtg cgaccagtac gtaccttccc cgtgggctga gcgaccgccg
     1321 gtaatggaga accgagtgtt ggtccacaat gtcgaggatc aggtcgtact tcgccccgtt
     1381 cgtggtgaag tcttcgcggg tgtaatcgat cacgtggtcg gcaccggccg agcgcatcaa
     1441 atcgagcttc tcggtgtggt cgactccagt cacctcggct ccggatgcct tggcgatctg
     1501 tatcgcgaaa ggcccgacac agccaccggc gccgatgatg agcacccgtt ggccaggctg
     1561 gatttcaccc ttgcctcgaa gtccctggag tgccagaacg gccgagtgag gaacggtggc
     1621 tgcctcctca aaggtaaggc cggcaggctt ggttgaaaac gcgctcttag aagcacagac
     1681 aaactccgag aaagccccat ggccatagga gaacatgtcg gcccagacct cgtcgcccgg
     1741 ctgcagggag gtcacattct tcccggtcgc ctcgactcgg ccggcaatat ccagtccgag
     1801 catcctgtgt ttcggtcgga gaagcccggt cccaatgcgg gtcatcaccg gtcgccctct
     1861 caataagtcg agatccgccg tattcaggga tgccgcgtga actctcacca gcacctcgcc
     1921 gtcacccggc actggcttgg ccacatcctc gaaccgaaga acaccgggag agccgtactc
     1981 gtctcggacg atcgccttca tgatgcccca gccaataggt ctcgttcaga cggtgacgac
     2041 gactttgcca agcgcgtgcc cctctcccac ataacgaact g
//
LOCUS       DBNHXU010000286         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1089554, whole genome shotgun sequence.
ACCESSION   DBNHXU010000286 DBNHXU010000000
VERSION     DBNHXU010000286.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1089554"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            45..278
                     /locus_tag="AC161X_07290"
     CDS             45..278
                     /locus_tag="AC161X_07290"
                     /inference="COORDINATES: protein motif:HMM:NF023092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3263 domain-containing protein"
                     /protein_id="HIR4989391.1"
                     /translation="MLSTEEKAVLEFESSWWLQPGPKDQAIEFSLGLTAAGYYEALLA
                     LVDRQEAMRHDPLTVKRVLSMIVTSSEPAAGVG"
     gene            278..823
                     /locus_tag="AC161X_07295"
     CDS             278..823
                     /locus_tag="AC161X_07295"
                     /inference="COORDINATES: protein motif:HMM:NF024791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LytR C-terminal domain-containing protein"
                     /protein_id="HIR4989392.1"
                     /translation="MAGKGRHASKSSGAFYRDLLFMTVGIILVGAAVFLLLYLLAGDR
                     TPEAAGTTTSTTNTTTEQGPTTTAQATTSTTSVSTTAATVPLRAPGEVRVMVFNSLPL
                     SGAAGRFTQELADAGYQTLPADDLDPEYDPSRIWYREGFSAEANALLSLLSGATVEPL
                     PDPTMGEGADLVVVLGVGYEE"
     gene            820..1680
                     /locus_tag="AC161X_07300"
     CDS             820..1680
                     /locus_tag="AC161X_07300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989393.1"
                     /translation="MTHTASGSGRGFPRAWLLPMVALVGILIYAALGWLSRSVVIDFL
                     SWWPLWLLVVGVGFLTRGKRIGKMRLSGLVPLLALAALGVFLAGHLLGWDVMPSSVPP
                     LVGPASDSVTTAAMSARIDGRLDVGLGQTGLLYSAESIRTGGDIGLPEAVERTQGTTI
                     AVVLESVDDPGLFTFSGWSLELDPSPAWALSLGGELSADLSAIRVTELQVQGRGSVRL
                     GTPMSEAPVSVSGDFEITVPGGAAVRVVGVAEVPGDWIETADGWESPAGTGGWVVSVT
                     EGSTLAIREG"
BASE COUNT          272 a          536 c          558 g          341 t
ORIGIN      
        1 ccaaatgccc gagtatcaga gatgggagaa ataccctcag accaatgctc agcactgagg
       61 agaaggctgt tctcgaattc gaaagctcgt ggtggctcca acccgggccc aaagatcagg
      121 ccatcgaatt cagcctcggt ctcaccgctg ccggttacta cgaagccctt ttggccctgg
      181 tcgatcgcca ggaggcgatg cgccacgatc cgctgaccgt gaagcgtgtg ttgtcaatga
      241 tcgtcacctc gtcggagccg gcagcaggag tcggctgatg gccggaaagg gcaggcacgc
      301 ctccaagtcg tccggcgcct tctaccggga cctgttgttc atgacggtcg ggatcatcct
      361 ggtaggtgcc gcggtgttcc tcctcctgta cctactggcg ggcgaccgca ctccagaagc
      421 ggctgggacc acgacttcca ccaccaacac aactacggaa caaggtccaa cgacgaccgc
      481 tcaggcgacg acctcgacta cctctgtctc gaccaccgcg gcgaccgtgc cgcttcgtgc
      541 cccaggggag gtgcgagtga tggtgttcaa ctccctgccg ctgtcgggtg cggcgggacg
      601 cttcacccag gagctagccg acgccggcta ccagactctg cccgccgacg atctcgaccc
      661 cgaatacgac ccgtctcgca tctggtaccg cgagggcttc tcggccgagg cgaatgcgct
      721 tctcagcctt ttgtcaggag caacggtcga gcccctgccc gacccaacca tgggcgaagg
      781 ggcagatctc gttgtcgttc tcggcgtcgg ttacgaagaa tgacccacac cgcctcgggc
      841 tccggccggg gatttccaag ggcctggctg cttccgatgg tggctcttgt cgggatcttg
      901 atctatgcgg ctctcggctg gctcagtcgt tccgtggtga tcgacttcct gtcctggtgg
      961 cccctttggc ttctggtcgt cggggtcggc tttctgaccc gaggcaagcg gattggaaaa
     1021 atgcgtctct ccgggctggt gccgctcctg gcgctggctg ccctgggagt gttcctggcg
     1081 ggccacctct tgggctggga cgtgatgccc tcctcggtcc ccccactggt cggcccagcc
     1141 tccgactcgg tgacgaccgc ggccatgtca gccaggatcg acggtcgtct cgatgtgggc
     1201 ctaggccaga ccggccttct ctattcagcc gagtcgatcc gaaccggcgg cgacatcggg
     1261 ctgcctgagg cggttgagcg aacccagggg acgacgatcg cggtcgtcct ggagtcggtc
     1321 gatgacccgg ggctattcac cttttctgga tggagtctgg agctagatcc ctccccggcc
     1381 tgggcactct cgttgggagg tgagttgtct gctgatcttt cggcgatccg ggtgaccgag
     1441 ctccaggtgc agggtcgagg ctcggttcgg ctgggcacgc cgatgtccga ggcgccggtt
     1501 tcggtctccg gcgactttga gatcacagtg ccaggagggg cggcggtgag ggtggtgggc
     1561 gttgcagaag tgcctgggga ctggatcgaa accgccgacg gctgggaatc tcctgccggc
     1621 accggcggat gggtggtctc agtgaccgaa gggtcgaccc tggccattcg agagggctag
     1681 ttacactcgt cgaatcgtgc cctctga
//
LOCUS       DBNHXU010000287         2586 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3866739, whole genome shotgun sequence.
ACCESSION   DBNHXU010000287 DBNHXU010000000
VERSION     DBNHXU010000287.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2586)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2586
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3866739"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1686
                     /locus_tag="AC161X_07305"
     CDS             <1..1686
                     /locus_tag="AC161X_07305"
                     /inference="COORDINATES: protein motif:HMM:NF015714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HIR4989394.1"
                     /translation="SNGLPGLARGQPYRSTIRVSALDVFGGSPSPIVRMGQGLRDRVM
                     ERLEPLDDSRALLAGFLIGETSGLDDIDVMAMRRAGISHFTAVSGSNVALFLGLLAVV
                     SGPLGVGPKRRAVVGLIGLPVYAAATRFEPSVMRASVMAAGVLVGRLFGMALEAWQLL
                     SAAVVGLLLFDPRLASDVGFQLSVAATAGVLIGARWPLAPGHWRRALAVTVGAQIAVA
                     PILIISFDRVPLLSPLVNLIAAPIVSAATVVGAVGVGGVGPLVGIAAWLSGIVLDLAR
                     GVSTWPQIGVVGLLGIGGAAVAFIRFRSLRGPLAMAAALVFVAGFLGPQSSLPNPGVV
                     VLDVGQGDSILISGGDGRLALVDGGPDQVRLIEKMGEYGVRHLELVVLTHVHADHAAG
                     LVGLAGRIPIGHVWAITDHHHTPASDELFLRLGAARVPISEPGVGQQWDLGELTLTVL
                     GPLRRYAGPNDESIVVMVSGVSRSMLLTGDIEVIAQDELGPIGVDVLKVPHHGGGTSD
                     AKWLEQVGADVAVISVGENDFGHPVDWVMSTLEESGAVVKRTDIDGDVVIELS"
     gene            1715..2527
                     /locus_tag="AC161X_07310"
     CDS             1715..2527
                     /locus_tag="AC161X_07310"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF013007.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR4989395.1"
                     /translation="MEGRSSIQLGDVALPLGDLTHVMGVINMSPDSKNLHTVAHSVDE
                     ALLMADRYRTWGGSLIDLGGQSSHFDNPTIDEVDEISRVVPVIEALAAEGHLVAIDTW
                     KAEVARAALNAGAVIVNDTGGLRDPEMRSLVAETGAAAIAVYVEGSNPHDVGEIEIRV
                     DKARHTAQEFAGLLEDLEGEGIRNVILDPGIALNYRGDYEAYTRMQLEVIASSEELHR
                     LGVPLLIPIPRKRDFHRVVAYMALALEHRADIIRVHDVAVACDLAKLFGRSP"
BASE COUNT          400 a          750 c          884 g          552 t
ORIGIN      
        1 agcaatggct tgccgggcct ggctcgaggg cagccctatc gcagcaccat cagagtttcg
       61 gccctcgatg tcttcggcgg ctccccctcg ccgatcgtga ggatgggaca aggtttgcgg
      121 gatcgggtga tggagcgcct ggagcctctc gatgactccc gggccctcct cgcaggtttt
      181 ctgatcggcg agacgagtgg gctcgacgac atcgatgtca tggcgatgcg tcgcgccggg
      241 atttctcatt tcacggccgt cagcggttcg aatgtcgccc tcttcctcgg cctgttggcg
      301 gtcgtctccg gcccactcgg tgtcgggccc aagcggaggg cagttgtggg cctcatcggg
      361 ctgccggtct atgccgcagc caccagattc gaaccttctg tgatgagggc ctcggtgatg
      421 gcagcggggg tgttggtcgg acgactgttt ggaatggcac tggaggcgtg gcagcttctc
      481 tcggctgcgg tggtcggatt gctcctgttc gacccgcgtc tcgcttcgga tgtcggcttt
      541 caactgtcag tcgcggcgac cgcaggggtg ctgatcggtg ctcgatggcc tctagctccg
      601 ggtcactggc gtcgagcgct cgcggtgaca gtcggtgcac agatcgcagt tgcaccgatt
      661 ctcatcatca gtttcgatcg ggtcccactg ctctcgccgc tggtgaatct gattgcggct
      721 ccgatcgtca gcgccgcgac cgttgtcggc gcagtgggtg tcggcggagt tggtcctctg
      781 gtcgggatcg cggcgtggct ctcgggaatc gtcctcgatc tggcgcgggg tgtctctacg
      841 tggccccaaa tcggggtggt cgggctcctc ggcatcggcg gtgctgcggt ggccttcatc
      901 cggtttcgtt cgctgcgtgg gccgttggcg atggcggcgg ccctggtgtt cgtcgccggg
      961 ttcttgggcc ctcagtcgtc actgccgaac cccggagtgg tggttctgga tgttgggcaa
     1021 ggagactcga tcctgatatc cggaggtgac ggccgactcg ccctcgtcga cggtgggccg
     1081 gatcaggtgc gcctgattga gaagatgggg gagtacgggg tgcgacatct cgaattggtg
     1141 gtcctgaccc acgttcacgc tgaccatgcc gccggtctcg tcggtctggc aggtcgaatt
     1201 ccgattggtc atgtctgggc gatcaccgat caccatcaca cgccggcgtc cgacgagctg
     1261 tttctgcgtc tcggagcggc gcgtgttcca atctcggagc cgggagtggg ccaacaatgg
     1321 gatctcggcg agctgactct caccgtgctc gggccgcttc gtcgatacgc cggtcccaat
     1381 gatgagtcga tcgtcgtcat ggtgagcggt gtctcgcgat cgatgctgct cactggcgac
     1441 atcgaggtca tcgcccagga cgagttggga ccgattggcg tcgacgtgct caaggtcccg
     1501 catcacggtg gcggcacttc ggacgccaaa tggttggagc aggtcggggc tgatgttgcg
     1561 gtcatctcgg tcggcgagaa cgacttcggc catcccgtgg actgggtgat gtcgacactc
     1621 gaggagtcag gagcagtggt gaaacgcacc gacattgacg gagacgtggt gatcgagctt
     1681 tcgtgagcga aacgtggatg ggaaactgcc agggatggaa gggcgatctt cgattcaact
     1741 gggagatgtg gctctgcctc ttggcgacct cacccatgtg atgggggtca tcaacatgtc
     1801 gcccgactcc aagaatctcc acactgtggc ccactcggtt gatgaagccc ttctgatggc
     1861 agaccgctat cggacctggg gtgggtccct gatcgatctc ggtggccaat cctcacattt
     1921 cgacaatccg acgatcgatg aggtggacga gatctcccgg gttgtgccgg tgatcgaggc
     1981 cctcgctgcc gagggccacc tggttgcgat cgacacctgg aaggccgagg tcgcacgggc
     2041 cgccctcaat gccggagcgg tcatcgtcaa cgacaccggc ggtctccggg acccggagat
     2101 gaggtcgttg gtggccgaga ccggagcggc ggcgatcgcg gtctatgtcg agggatccaa
     2161 cccgcatgac gtaggggaaa tcgagatccg ggtggacaaa gcgcggcaca cggcccagga
     2221 gttcgctgga ttgctggagg acttggaggg ggaggggatc cgcaacgtga ttctcgatcc
     2281 ggggatcgcc ctcaactacc gcggggacta cgaggcctac acccggatgc aactcgaggt
     2341 gatcgcctcc agtgaggagt tgcaccgtct tggcgttcct ctcctcatcc ctatccctcg
     2401 aaaacgagac ttccacaggg tggtggcgta catggcgctg gccctcgagc accgagccga
     2461 catcatcagg gtgcacgatg ttgccgtcgc ctgtgacctc gccaagctgt tcggccgatc
     2521 gccttaagtg tcgttgggtc tgactacggt ctgagaaatg ggtttgttcc tcatctcggc
     2581 gccctc
//
LOCUS       DBNHXU010000288         3245 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4661217, whole genome shotgun sequence.
ACCESSION   DBNHXU010000288 DBNHXU010000000
VERSION     DBNHXU010000288.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3245)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3245)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3245
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4661217"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..872)
                     /locus_tag="AC161X_07315"
     CDS             complement(<1..872)
                     /locus_tag="AC161X_07315"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR4989396.1"
                     /translation="MKFALRLTTLGIVFTAMLAVIGLRLWFVQVAEGPQTAQAAEDLA
                     WLDKTSYGPRGDIVDRNGTLLATSRLVPAVYIDRTFVQPDDQSSLIQTLSSITGFDPI
                     ELGRMYEAAGINGRFEVVTVSNETAYAINENLDHLPGVEIVMVPERVYISGPTLAHVI
                     GHLGLPDAADKEARPDLDLSQRIGKLGVEKVYDQYLQGVSGLVQYQVQRGEIVDQKPT
                     VEPGPGDSVVLNIDYDLQDLVELGLEEGVNLSNSVKDEARANGDDVFSETQRAAAVVL
                     DAKNFEVLALASYP"
     gene            complement(869..1372)
                     /gene="mreD"
                     /locus_tag="AC161X_07320"
     CDS             complement(869..1372)
                     /gene="mreD"
                     /locus_tag="AC161X_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HIR4989397.1"
                     /translation="MRGRSAVIVVLLFVVAVVVQTTLFAQINLVTPDLVLLLVIILCL
                     TKIRPEAILAIAFTSGLVVDLLGPSALGLRAVVYTTVAYIGIRTRDRADIGRVAVALW
                     AGVLTLIGVILLMLIGTLFGQSSLLGENTLSRLILVPLANLAIAAAVAPLVVRLVDRD
                     KTAFRYP"
     gene            complement(1372..2289)
                     /gene="mreC"
                     /locus_tag="AC161X_07325"
     CDS             complement(1372..2289)
                     /gene="mreC"
                     /locus_tag="AC161X_07325"
                     /inference="COORDINATES: protein motif:HMM:NF016011.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005618 - cell wall [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HIR4989398.1"
                     /translation="MLSRESSNRLLPTFITLLVVGVLLMTIDVRLEGKGVVGVLRSGT
                     QSIVAPLQEAASLVVNPVADMFDSLTNVASLREQNISLRAELAEAQAELVGVQDRLAL
                     LALLEQLYGLDTADPSLGQTVANVIGKVESAFVVDKGTNDGVVVGQPVIDTNGYVVGT
                     VRTVTSGSATIVPITTGRNGITVLVNVQVGTLTPQVNTSEMRLLIGDAREPVLEGDRV
                     VTSAGSISYPAGLPVGVVLHDAAPESHSLTASVEPYVNTDTLRIVVILAWPPDPVTAA
                     TDDAGDVEETTTTTTDASTTTTSTLENEG"
     gene            complement(2312..>3245)
                     /locus_tag="AC161X_07330"
     CDS             complement(2312..>3245)
                     /locus_tag="AC161X_07330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009739818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HIR4989399.1"
                     /translation="NEPSVVAINTHNGRALAVGTEAKRMIGRTPAHITAIRPLRDGVI
                     ADFDITEKMLRYFIQKVHRRKWARPRIVVCVPSGITPVERRAVEEAAYHAGARRAYTI
                     EEPMAAAIGSGLPIGEPAGSMVVDIGGGTTEVAVISLGGIVVSRSIRVGGDEMDTSII
                     DWVKKEYNVLIGERTAEQLKLAVGSAWPYADEPSAEVRGRDLVTGLPKTILLSSPEIR
                     EAIEEPTQAVVDAVKFTLDKTPPELAADVVERGIVITGGGALLVGLDHRLAHETGMPI
                     VTAAKPLQSVVIGSGKCLEEFDTLQNVLVSSSRP"
BASE COUNT          635 a         1010 c         1014 g          586 t
ORIGIN      
        1 tccgggtagg aggcgagagc caggacttcg aagttcttgg cgtcgagcac gaccgcagcc
       61 gctcgctggg tctcactgaa cacgtcgtcg ccgttcgccc gtgcttcatc cttgaccgag
      121 ttggagagat tcacaccctc ttcgaggccg agctccacga ggtcctgaag gtcgtagtcg
      181 atgttgagca cgaccgagtc gccaggcccg ggctcgaccg tgggtttctg atcgacgatt
      241 tcgcctcgct gcacctggta ttggaccaac ccggagaccc cctgcaggta ctggtcatag
      301 accttctcca ccccgagttt cccaattcgt tgactcaggt cgagatcagg cctcgcctct
      361 ttgtccgcgg cgtcgggcaa accgagatgg ccgatgacat gggcgagggt cgggcctgag
      421 atgtagaccc gttccgggac catcactatt tccacgcccg gcaggtgatc gagattctca
      481 ttgatcgcat aggcggtctc gttggacacg gtgaccacct cgaaccgccc attgatgcca
      541 gccgcctcat acatgcgccc gagctcgatc ggatcgaacc cggttatcga cgagagggtc
      601 tggatcagcg agctttggtc gtcaggttga acgaaggtgc gatcgatgta caccgccggc
      661 accaaacgac tggtggcaag caaggtcccg tttcggtcga cgatgtcgcc acgcgggccg
      721 tatgacgtct tgtcgagcca tgccaggtcc tcggcagctt gagccgtctg cggcccttcc
      781 gccacctgga cgaaccagag tcggaggccg atcaccgcga gcatcgccgt gaagacgatg
      841 ccgagggtgg tgagtctgag tgcaaacttc atgggtatct aaacgccgtc ttgtcccggt
      901 ctacgagacg caccaccagt ggagcgacgg ctgccgcgat agcgaggttg gccaacggta
      961 cgagtatcag gcggctaagt gtgttttcgc caagcaagct cgactggccg aagagggttc
     1021 cgatcaacat cagaaggatg accccgatca gtgtgaggac acccgcccat agggcaaccg
     1081 ccacccgtcc gatgtccgcc cggtccctgg tccgaattcc gatgtaagca acagtggtgt
     1141 agacgacggc gcgcaatcca agcgccgacg ggcccagtag atccaccacc aaccccgagg
     1201 tgaaggcgat tgcaaggatg gcctcggggc ggattttggt cagacacagg atgatgacga
     1261 gaaggagaac caggtctgga gtgaccaggt tgatctgggc gaacaacgtc gtctgaacca
     1321 ccactgcaac gacgaacagc agcacgacga tgactgccga ccgcccacgc atcagccctc
     1381 attctccagc gtcgaggtcg tggtcgtaga ggcgtcggtc gtggtcgtgg tcgtctcctc
     1441 gacatcgccg gcgtcgtcgg tggcagcggt caccgggtcc ggtggccagg caaggatgac
     1501 cacgattcgc aaggtgtcgg tgttgacata cggctcgaca cttgccgtca atgaatggga
     1561 ctcgggcgcg gcgtcatgaa ggacgacacc gaccgggagc ccggccggat aggagatgct
     1621 gccggcggat gtcaccaccc gatcgccctc gaggaccggc tcacgggcat ccccgatcag
     1681 aagtcgcatc tccgatgtgt tcacctgtgg tgtcagggtt ccgacttgaa cgttgaccag
     1741 cacggtgatg ccgttgcgcc cggttgtaat cggaacgatc gtcgccgatc cggaggtgac
     1801 ggtcctcacg gtcccgacaa cgtagccgtt ggtatcgatg accggttgtc cgaccaccac
     1861 gccgtcgtta gtccccttgt ccaccacaaa cgcggactcg accttgccga tcacattggc
     1921 aaccgtctga cccaggctcg ggtcggcggt gtccaacccg tagagctgct ccaacaacgc
     1981 gagcagggca aggcgatcct gaaccccgac caactctgct tgcgcctcgg cgagctcagc
     2041 gcgaagcgag atgttctgct cacgcaggga ggcaacgttg gtgagactgt cgaacatgtc
     2101 tgccacgggg ttgaccacca acgacgccgc ctcctgaagc ggggcgacta tggactgagt
     2161 gcctgagcgg aggactccca cgacgccttt gccctcgagg cggacgtcga tcgtcatcaa
     2221 gagcacacca accaccaaaa gagtgatgaa ggtggggaga agccggttgg acgattctcg
     2281 gctcagcatc ggtggagacg ggtcagggcg ttcacggccg cgacgaggag acgagaacgt
     2341 tctgcaacgt gtcgaattcc tccaggcact tccccgagcc gatcaccacc gactgcagcg
     2401 gcttggccgc cgtgacgatg ggcatccccg tctcatgggc caatcgatga tcaagcccaa
     2461 ccaagagagc cccaccaccc gtgatgacga tcccgcgttc tacgacgtcg gccgccagct
     2521 cgggaggggt cttgtctagc gtgaacttca ctgcgtccac gacggcctga gtcggttcct
     2581 cgatggcctc gcggatctcc gggctcgaga gaagaatggt cttgggcaac ccggtgacca
     2641 ggtcgcggcc gcgcacctca gccgaaggct cgtcggcata cggccaagcc gagcccacgg
     2701 cgagcttgag ctgctctgcg gttcgctcgc cgatcaggac gttgtattcc ttcttgaccc
     2761 aatcgatgat cgaggtgtcc atctcgtcgc cacccacgcg aatcgagcga gagacgacga
     2821 tcccacccag ggagatgaca gcgacttcgg tggtgccacc accgatgtcg acaaccatcg
     2881 aaccggcggg ctcgccaatg gggagacccg atccaatggc tgcggccatt ggctcctcga
     2941 tggtgtaggc ccgtcgagca cccgcgtgat aggcagcctc ttcgacggcg cggcgctcga
     3001 ccggggtgat ccccgaaggg acacaaacca cgattcgagg ccgtgcccac ttgcgtcgat
     3061 ggaccttttg gatgaagtaa cgaagcattt tctcggtgat gtcgaagtcg gcgatgaccc
     3121 catccctgag cggccggatg gcagtgatat gggccggggt gcgcccgatc atccgtttcg
     3181 cctccgtccc gacggcaagg gcgcggccgt tgtgggtgtt gatggcgacc accgacggct
     3241 cgttg
//
LOCUS       DBNHXU010000289         1938 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2281129, whole genome shotgun sequence.
ACCESSION   DBNHXU010000289 DBNHXU010000000
VERSION     DBNHXU010000289.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1938)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1938
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2281129"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1742)
                     /locus_tag="AC161X_07335"
     CDS             complement(<1..1742)
                     /locus_tag="AC161X_07335"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4989400.1"
                     /translation="MSTDPSLPQVSIVIPAYNRSEVLAKTLSALTAQNYPASSLTVVV
                     ADDGSSENVEAVVDAWDPPFSKLYVRQDHDGFGAGRARNLGAGAVPSEVIIFLDSDAL
                     VSPDFVSKHAAWHVDDPKSVVIGGRVHLSSTGLSAEQLETAAVDLGAQPFEDRADFRS
                     VLTRRTSRLQSTDEGYRAFVSSNVSLATSLFESTGGFDARFRWWGSEDSEFGWRLWQT
                     GATFVADETARIYHQVDSDTAGGEEGRQQARELNRGLLASLVPQRFYRKGMPEPPPEV
                     PKFSILVHDIPEAAAKSWWRSMIGQTLPDFELVFLADGASHDPFAGAAEGERRISFVE
                     DLESAINTSRGEYLVVLDGYAAPGPSLLQNLRKRLDDRPAATGLTFGIETPTGALSRI
                     EDLHQLQEDWGTELPLAFAVRRRPLMRLLDQKVDPSVAISDLHVGADTIHTAQVLVAL
                     PGASRTERPPGFSFAKSQSRQLFEATQLGAGPALQTGLRLVKHRLRPPRPIREKAPVT
                     ADRIPGIRYVGWVGKDNLGDEAMLDATRDLLSWGDVDVRGEAKDLLLLGGGTLINRNQ
                     YLKWLIERDSPRIER"
     gene            complement(1739..>1938)
                     /locus_tag="AC161X_07340"
     CDS             complement(1739..>1938)
                     /locus_tag="AC161X_07340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989401.1"
                     /translation="DDLSGLEDLIRSARRYIYIEGAAKGLVEFLDHEAPRASVEERDG
                     VTRVDLMAPSYRIMSFEVRDS"
BASE COUNT          354 a          579 c          648 g          357 t
ORIGIN      
        1 cgctcgatac ggggcgagtc gcgctcgatc agccatttca aatactggtt tcgatttatg
       61 agagtgcctc cgccgagcag caagaggtcc ttcgcctcgc ccctgacgtc gacgtcaccc
      121 caagagagca ggtcacgggt ggcgtcgagc atggcttcat cacccagatt gtctttgccc
      181 acccagccca cgtaacgaat gcccgggatc cggtcggcag tgaccggcgc cttctcgcgg
      241 atgggccgtg gtgggcggag tctgtgcttg accaggcgaa gacccgtctg gagcgcgggc
      301 cctgccccga gttgagtggc ttcgaagagc tgacgtgact ggctcttggc aaatgagaaa
      361 ccggggggtc gctcggtgcg cgatgccccg ggcagggcga caaggacctg ggcggtgtgg
      421 atggtgtctg caccgacgtg aaggtcagag atcgccacgg acgggtcgac cttctggtcg
      481 aggagtctca tcaatgggcg gcgccgcact gcgaacgcca gaggcaactc ggtgccccag
      541 tcctcttgca gttggtggag atcctcgatt cgggaaaggg cacccgtcgg ggtctcgatg
      601 ccaaaggtca gaccggttgc ggcggggcga tcgtccaacc tctttctgag gttctggagc
      661 aatgacggtc cgggggcggc atacccgtcg aggacgacca ggtattcgcc tcgcgacgtg
      721 ttgatggcag attccagatc ctcgacaaac gagatccgcc gctcaccctc agccgctcct
      781 gcgaagggat catgtgaagc gccgtccgca agaaacacga gttcgaagtc gggcagggtc
      841 tggccgatca tcgagcgcca ccaggacttg gcggctgcct cggggatgtc atggaccaga
      901 atcgagaact tgggaacttc gggcggaggc tcaggcatgc cttttcggta gaaccgttga
      961 ggaacgagac tcgccagcag cccccgattg agctcgcggg cctgctgtct cccttcctcg
     1021 ccgcctgccg tgtccgagtc gacctggtga tagatccgtg ctgtctcgtc ggcgacgaag
     1081 gttgctccgg tttgccacag acgccagcca aattcggagt cttcactgcc ccaccagcgg
     1141 aatctggcat cgaacccgcc ggtcgactcg aaaagggagg tggcgaggga gacgttcgag
     1201 gatacaaatg cccgatagcc ctcatcggtg ctctgaaggc gggaagtgcg gcgggtgagc
     1261 accgacctga aatcggcgcg gtcctcgaat ggctgagcgc cgaggtcgac agctgccgtt
     1321 tcgagttgct cggcgctcag gccggtcgag ctgagatgca cccttccccc gatcaccacc
     1381 gatttgggat cgtcgacatg ccaggcggcg tgtttcgata cgaagtcggg ggagacgagg
     1441 gcgtccgagt cgaggaagat gatgacttcg gatggcaccg cacctgcgcc gaggttgcgg
     1501 gccctccccg cgccaaaccc gtcatggtcc tggcggacgt agagcttgga gaagggaggg
     1561 tcccaggcgt cgacgaccgc ctccacgttc tcggatgagc cgtcgtcggc cacgaccaca
     1621 gtgagggagg aggccgggta gttctgcgcc gtcagggcac tcagggtctt tgccaggact
     1681 tcgctgcggt tgtacgccgg gatgacgatt gagacctgag gcaacgacgg gtcggtgctc
     1741 acgagtctct cacttcaaac gacatgatcc gatagctggg ggccatcagg tccactctgg
     1801 tgacgccgtc gcgttcctcg acggacgctc ggggtgcttc atgatccaag aattcgacga
     1861 gcccctttgc cgctccttcg atgtagatgt agcggcgcgc ggaacggatc aagtcctcga
     1921 gccccgatag gtcgtcgc
//
LOCUS       DBNHXU010000290         1986 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1291814, whole genome shotgun sequence.
ACCESSION   DBNHXU010000290 DBNHXU010000000
VERSION     DBNHXU010000290.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1986)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1986)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1986
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1291814"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1496
                     /locus_tag="AC161X_07345"
     CDS             <1..1496
                     /locus_tag="AC161X_07345"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR4989402.1"
                     /translation="AEHIGPETEVVDLDGRLLTPGFQDSHVHVWSSGLDLLRCSFAGC
                     GNGADAIAYVANYARANPNVEWIVGGGWHQTWFPRACPSKEVLDEIVPDRPVFLYNAD
                     GHGAWVNSRALELAGIDASTPDPFDGRIERNPDGTPQGTLHDGAANLIEAASPKDTLE
                     EALRGILAGQEYMLSKGITGWQDTHVDQLAHAAYRALAQRDQLRGTAVGALWWDRHRG
                     LDQIEDLERMRGEGIGRYQPVAVKLMADGVVETQTAAMLEPYLDETGAITANRGIDFI
                     DPGELKEIVVALDGRGFSCHFHAIGDAAVRNSLDAVEAARMANGWSAARHTICHVQVV
                     HPDDLHRFGKLGVAVSAQALWAGNGQDQLELTQPFLGPERTMWQYPFGSLLRAGATLA
                     MGSDWAVSTANVLAQSDMAVNRLNWTDPGHPPLNPDERINLVDALAGFTSGSAWVNHR
                     EVDSGTVAVGMLADLVVLDRDPFQFGPIRDARVAMTVVGGQVVYEED"
     gene            1496..>1986
                     /locus_tag="AC161X_07350"
     CDS             1496..>1986
                     /locus_tag="AC161X_07350"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014435016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989403.1"
                     /translation="MVNAAVELSGVTKRFSDDFVAVDGLDLRINDGEFFSLLGPSGCG
                     KTTTLRMIAGFEFPTQGSIRVHGVEMALNPPNERPINTVFQSYALFPHMTVAENVAFG
                     LQMKKVEKSEVAGRVKRVLDQVQLGKRADAKPNQLSGGMQQRVALARALVNEPEVLLL
                     DEPL"
BASE COUNT          365 a          613 c          651 g          357 t
ORIGIN      
        1 tcgcggagca catcggcccc gagaccgagg tggtcgatct cgacggccgg ctcctgaccc
       61 cagggttcca ggactcacac gtgcatgtgt ggagtagcgg tctcgatctc ctgagatgtt
      121 ccttcgcggg ctgtggaaat ggcgccgatg caatcgccta tgtcgctaac tatgcccgag
      181 cgaaccccaa cgtcgagtgg atcgtcgggg ggggatggca ccagacctgg ttcccacgag
      241 cctgcccgtc gaaggaggtg ctggacgaga tagttcccga ccgcccggtg ttcctgtaca
      301 acgccgacgg tcacggggcg tgggtcaaca gccgtgccct cgagttggcc gggatcgacg
      361 cgtccacgcc agaccccttc gatggccgga tcgagcggaa ccccgatggc acgccccagg
      421 gcacgctgca tgacggcgcc gccaacctca tcgaagccgc ttcaccgaaa gacaccctgg
      481 aggaggcgct ccggggcatc ctcgccgggc aggaatacat gctctccaag gggatcaccg
      541 ggtggcagga cacccacgtc gatcagttgg cccacgccgc ctaccgggcc cttgcccagc
      601 gtgaccagct acgaggcact gcggttggtg ccttgtggtg ggatcgacat cggggactcg
      661 atcagatcga agatctggag cggatgagag gggaagggat tggccgctac cagccggtgg
      721 ccgtcaagtt gatggccgac ggagtagtcg aaacccaaac ggccgccatg ctcgagccct
      781 acctcgacga gaccggagcc atcacggcca accgcgggat cgacttcatc gaccccggcg
      841 agttgaagga gattgtcgtc gcccttgatg ggcgcggatt ctcatgccac ttccatgcga
      901 taggcgacgc tgccgttcgg aattccctcg atgcggttga ggcggcgcga atggccaatg
      961 gctggtcggc ggcgaggcac acgatttgtc atgtacaggt ggtccacccc gacgacctcc
     1021 accgtttcgg caaattgggc gttgcggtca gcgcccaggc cctttgggcc ggcaacggcc
     1081 aggatcagct cgaactgacc cagcccttcc ttggcccgga gcggacgatg tggcaatacc
     1141 cgttcgggag cctgctgcga gccggggcca ccctggccat gggctcggat tgggcggttt
     1201 cgaccgcgaa tgtcctggct caaagtgaca tggccgtgaa tcggttgaat tggaccgatc
     1261 caggccatcc gccactcaac cccgatgagc ggatcaacct tgtcgacgcg ctcgccgggt
     1321 tcacctcagg ctcggcctgg gtcaaccacc gcgaggtcga ctccgggaca gtcgccgtgg
     1381 gtatgctcgc cgacctggtg gtcctagatc gggatccgtt ccagttcggg ccgattcggg
     1441 atgcaagagt cgcaatgacg gttgtgggcg gacaggtggt ttacgaggag gactgatggt
     1501 caacgcggcc gttgagttga gcggtgtcac taagcgcttc agcgatgact ttgtggcggt
     1561 cgatgggttg gacctccgca tcaacgacgg cgaattcttt tccttgctcg gcccttcggg
     1621 gtgtggcaag accaccacgc tccgcatgat cgccggattc gagtttccca cccagggcag
     1681 catccgggtc catggggtgg agatggcgct gaatcccccc aacgagcgac cgatcaacac
     1741 cgttttccag tcctatgccc ttttcccgca tatgacggtg gccgagaacg ttgccttcgg
     1801 cctccagatg aagaaggtgg agaaatcgga ggtcgccggg agggtgaagc gggtgctcga
     1861 tcaggtccaa ctcggcaaac gtgccgacgc aaagccgaac cagctctcgg gaggcatgca
     1921 acagcgagtg gccctcgctc gggcactggt caacgagccc gaagtcctgt tgttggatga
     1981 gcccct
//
LOCUS       DBNHXU010000291         2980 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2482651, whole genome shotgun sequence.
ACCESSION   DBNHXU010000291 DBNHXU010000000
VERSION     DBNHXU010000291.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2482651"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="AC161X_07355"
     CDS             complement(<1..425)
                     /locus_tag="AC161X_07355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017832304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HIR4989404.1"
                     /translation="MSERPIPAILCTADGATFQGVSVGAEGVATGEAVFNTSMTGYQE
                     ILTDPSYAGQVVVMTSSHIGNYGLTDLDAQSSKAAVSGFAMRAISRRDSNWRSEGAIA
                     EYLVNHGVVAIADLDTRRLTRHLRERGAMPVAIGANVDEA"
     gene            complement(418..1686)
                     /locus_tag="AC161X_07360"
     CDS             complement(418..1686)
                     /locus_tag="AC161X_07360"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011720135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HIR4989405.1"
                     /translation="MIYVRGARVWGPAGLTETDVVIDSDRVAEIGARDVPAGARVIEA
                     SGAILGPGLVDLHVHLRDPGQTWKEDIASASMAAVAGGFTAIVAMPNTDPPMDNAKTV
                     NAVMERAAALGLVDIVAAGALTMGREGLEMADLDSMYEAGVRLFTDDGDTVADSGLLR
                     RIMTYLRDFPGAVVAEHAEEVTLSRGGHVNEGIVSASRGIVGLPAVAEEVVVARDLAL
                     ADDTGARIHIQHVSTRRSVDLMREAKRRGLAVTCEVTPHHLALDESAVASLDPNFKMY
                     PPLRTAADREALVEALEDGTVDAIATDHAPHTVAEKDVPFEEAPRGVIGLETAASVGW
                     AILKGDADLFFERMSVSPARIAGLERHGQPVERGSVANLVLFDPSRSWLPSGFHSKSA
                     NSPFAGAELKGMVVATIHNGSVVYEGPTDE"
     gene            complement(1683..2588)
                     /locus_tag="AC161X_07365"
     CDS             complement(1683..2588)
                     /locus_tag="AC161X_07365"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006502441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004070 - aspartate carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HIR4989406.1"
                     /translation="MSGLLTLRGISRSQLEELLDDAERHGKLIEGRDKGSSELRGATV
                     TTAFFEPSTRTRLSFEKAAFHLGAHVMTFSPESSSLEKGESLRDTLLTLTALGTDLFV
                     IRHKDVGVPSIAQRWTGLPVINGGDGRGEHPTQTLADALTLRKHFGALEGLKVGIVGD
                     IANSRVARGHLWAFPTLGIDLTLIGPRTLLPTANPWGARIEVELDSVIEKVDVLYMLR
                     VQRERGAGAGFPSIPAYVSRFGLTEARLARMQPSAVVLHPGPVNRGVEVCDAAVDGRR
                     SLVLEQVANGIPVRMAVLAAAMRAE"
     gene            complement(2585..>2980)
                     /gene="pyrR"
                     /locus_tag="AC161X_07370"
     CDS             complement(2585..>2980)
                     /gene="pyrR"
                     /locus_tag="AC161X_07370"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012120937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HIR4989407.1"
                     /translation="LVDSISSFEGVEVPVGELDIDPYRDDQGLRPARDARGTRIPVDI
                     TGKSVIVVDDVLYTGRTVRAGLDALSDIGRAAQTELAVLVDRGHREIPIRADYVGKNL
                     PTSVDEVVRVRLGEVDGDHGVWIDRMAVA"
BASE COUNT          530 a          995 c          922 g          533 t
ORIGIN      
        1 gcctcgtcga cgttggcccc gatcgcgacc ggcattgccc cccgttcccg taaatgcctg
       61 gtgagacgtc tggtgtcgag gtcggctatc gccaccaccc cgtggttcac gagatactcg
      121 gcgatggctc cctctgatcg ccagttggag tcccgacgcg agatcgctct catcgcgaat
      181 cctgagacgg ccgccttcga agactgcgcg tcgagatcgg tgagcccgta attcccgatg
      241 tgcgacgagg tcatcacgac cacctggccc gcgtaggaag ggtcggtgag gatctcctgg
      301 tagccggtca tcgacgtgtt gaacaccgcc tcacccgtgg ccacgccctc cgcaccaacg
      361 gagacacctt gaaaagtcgc accgtcggcg gtgcacagga ttgcggggat gggtcgctca
      421 ctcatcggtc gggccctcgt agacgaccga tccattgtgg atcgtggcaa cgaccattcc
      481 tttcagttcg gcaccggcga acgggctgtt ggccgacttt gagtggaacc cacttggcaa
      541 ccacgatcga gaggggtcga agaggaccag attggcgacc gaaccgcgct cgacaggctg
      601 gccatgccgt tctagaccgg cgatacgtgc cgggctgacg ctcatccgct caaagaacag
      661 gtcggcatcc cccttcagaa ttgcccatcc cactgaagcc gcggtctcga ggccgatcac
      721 tccccgaggg gcctcctcga aagggacgtc tttctcagcg accgtgtgcg gggcgtgatc
      781 tgtggcgatg gcgtcgaccg taccgtcctc caaggcctcc accaacgcct cgcgatcggc
      841 ggcggtgcgc aggggcgggt acatcttgaa attcgggtcg agactggcga cggcagactc
      901 gtccagagcc agatggtgcg gggtcacctc gcaagtaacc gctaggccgc gccgctttgc
      961 ctcgcgcatc agatccaccg aacgtcgggt tgagacgtgt tggatgtgga tgcgagcccc
     1021 ggtgtcatcg gcgagagcca gatcccgggc caccactact tcttccgcga cggcaggtaa
     1081 gccaacgatg ccacgactcg cggagacgat gccttcgttg acgtgcccgc ccctggacaa
     1141 tgtgacctct tcggcgtgct cggctaccac cgccccagga aagtcgcgga gataggtcat
     1201 gatccgccgc agcaggcccg agtcggccac cgtgtcgccg tcgtcggtga acaaccggac
     1261 gccggcctca tacatcgagt cgaggtcggc catctccagg ccttccctgc ccatggtcag
     1321 tgcgccggcc gccacgatgt cgacaagacc gagcgcggct gctcgctcca tgaccgcatt
     1381 caccgtcttg gcgttgtcca tcggtggatc agtgttgggc atggccacga tcgcggtgaa
     1441 cccgccagcg accgctgcca tggaggctga tgcgatgtcc tccttccagg tctgccccgg
     1501 gtcacgaaga tggacgtgaa ggtcgaccag gccgggcccg agaatcgctc ccgaggcttc
     1561 gatgacccgg gctccagccg gcacatctcg agctccgatc tcggcaacgc gatcgctgtc
     1621 gatgacgacg tccgtttccg tcagccccgc tgggccccac acccgggctc ctcgcacgta
     1681 gatcattcag ccctcattgc tgcggcgaga accgccatcc ggaccgggat gccatttgcg
     1741 acttgttcga gcaccagcga ccgccgcccg tcgactgcgg cgtcgcacac ctcgacacct
     1801 cggttgaccg gtcccggatg aaggaccact gctgacggct gcatgcgagc gagacgagct
     1861 tctgtcaggc cgaagcggct cacgtaggcc ggaatggatg ggaagcctgc tccggcccct
     1921 cgttccctct gcacccgcaa catgtagagc acgtcgacct tctcgatgac ggagtcgagc
     1981 tcgacttcga tccgggctcc ccaagggttg gcggtgggga gcaaggttct agggccgatc
     2041 aaggtgaggt cgatgccaag cgttggaaag gcccagagat ggcccctcgc caccctgctg
     2101 ttggcgatat ctccgacgat ccccaccttg agaccttcca gcgcgccgaa gtgcttcctg
     2161 agagtcaggg catcggcaag ggtctgggtt gggtgctctc ccctgccatc gccgccgttg
     2221 atcaccggaa gaccggtcca tcgctgggcg atcgatggga cacccacatc cttgtgccgg
     2281 atcacgaaca agtcagttcc cagcgcggtg agcgtcaaca aggtgtcgcg cagagactca
     2341 cctttctcca gcgatgaaga ttccgggctg aaagtcatga cgtgtgcccc gaggtggaag
     2401 gcggccttct cgaacgagag acgcgtccgg gtcgaaggct cgaagaaggc tgtggtcacg
     2461 gttgccccgc gtagctcgga gctgcccttg tccctgccct cgatcagctt gccgtgacgc
     2521 tcggcatcgt cgagcagctc ctcgagttgc gatcgggaga tgccacggag ggtgagcaaa
     2581 ccgctcatgc aaccgccatc cggtcgatcc aaaccccgtg atcgccatcg acctcgccga
     2641 ggcggacccg gaccacttcg tcgacactgg tcggcaggtt cttcccgacg tagtccgccc
     2701 gaatcgggat ctcgcgatgc ccccggtcca ccagcactgc cagctcggtc tgcgccgccc
     2761 gcccgatgtc ggaaagcgcg tcgagcccgg cacggaccgt tcgccctgtg tacaggacgt
     2821 cgtcgacaac gatcacgctc ttgccggtga tgtccaccgg gatccttgtc ccccgggcat
     2881 cgcgggcggg gcggagtcct tgatcgtcgc gataaggatc gatgtcgagc tccccgactg
     2941 gaacctcaac tccttcgaaa gaggagattg agtcgacaag
//
LOCUS       DBNHXU010000292         2797 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3671381, whole genome shotgun sequence.
ACCESSION   DBNHXU010000292 DBNHXU010000000
VERSION     DBNHXU010000292.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2797)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2797
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3671381"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..963)
                     /locus_tag="AC161X_07375"
     CDS             complement(199..963)
                     /locus_tag="AC161X_07375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015621865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SigB/SigF/SigG family RNA polymerase sigma
                     factor"
                     /protein_id="HIR4989408.1"
                     /translation="MTEREQEAIEDLFAVREQPEAKRELVEAFDGLAQRLARRFGGRG
                     VPLDDLVQVARFGLLKAIERFDADRGIQFSTFAGRTIIGEIKHYFRDQAWSVRVPRTL
                     QNLWLEVSRAVDELTHTLGRSPTIEEIAESLDVSEEEVLEALDAGAAYTASSLDRPLG
                     EDGDTTVVDQLGDLDPGYETAAVRGAMSAELRALPERERTVLYLRFFDGRTQAEIADE
                     IGVSQVHVSRILSRTLDQLREAIDPEAEDDSGDSDV"
     gene            complement(956..1291)
                     /locus_tag="AC161X_07380"
     CDS             complement(956..1291)
                     /locus_tag="AC161X_07380"
                     /inference="COORDINATES: protein motif:HMM:NF024970.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4989409.1"
                     /translation="MASLPHHVATVRMFVSAVARSLGLDVETIDDAKLAASEISTAIV
                     TAGDRSFITVRANLSPDGLTLEIGPWRDGLGGDEDFGPIDIAQALFEGTTVVGEFVLI
                     PIEAPGSDD"
     gene            1468..1782
                     /locus_tag="AC161X_07385"
     CDS             1468..1782
                     /locus_tag="AC161X_07385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989410.1"
                     /translation="MAGNAARLCGFSYNRVEEARLAVNEAASLLLADGRSSTVRCSLT
                     SDEGALFVEIEATPGPAIWPPEPWADSLEHTVLSSLANDLELVSDGGPMVRMTLGAQS
                     RG"
     gene            1876..>2797
                     /locus_tag="AC161X_07390"
     CDS             1876..>2797
                     /locus_tag="AC161X_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024808.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR4989411.1"
                     /translation="MTGALSKRLFPLGLVAVVGLITACGSGSSGSDETLVIYSGRAEE
                     LVSPLFARFTEETGIGVEIRYGGSSELAATLIAEGDGTEADVFFAQDPASLGTVDSMM
                     KVLDDATLDLVDPAFNDRDGHWVGISGRSRVVVYNTDTNVPLPQTIDDMTDPVWANQL
                     GIAPTNASFLAFVSAMILERGEDGTRTWLEALAANHPVEYESNAPIVEATDNGELEGG
                     LVNHYYLLRLRAEGLGEHAENYYIPAGDVGSLIMPAGVGIMSATDQPEPAQRFVDFLL
                     SEESQAFFATDTFEYPLIDGVPQPDGAPPLS"
BASE COUNT          516 a          912 c          837 g          532 t
ORIGIN      
        1 cgtgatacgc ggtcccgcgc cttgcccggc gcgaactcgt cgggtttcaa gacaagacgc
       61 atgcggtcaa tttccttggc gctggttgga ggagccgtac gcggcatcag gcgccaagtt
      121 aacgcccgcg tcgggcgatc ccgggttacc gactcctgtg agtgatttcg ggagatcact
      181 acccagagca gggccgccct atacgtcgga atcgccagaa tcgtcctctg cttcggggtc
      241 gattgcctcg cgaagctgat cgagggtccg actcaggatg cgtgacacgt gaacctgaga
      301 gaccccgatc tcatcagcaa tctcggcctg agtcctcccg tcgaagaaac gcaagtagag
      361 aactgttcgc tctcgttcgg gcaaagcccg cagttcggct gacatcgctc cccggaccgc
      421 cgccgtctcg tatccggggt cgaggtctcc gagctggtcc acgacagtcg tatccccgtc
      481 ctccccaagc ggtcggtcga gtgaactggc cgtgtaagcc gctccggcgt cgagtgcttc
      541 gaggacctcc tcttcggaaa cgtcgaggct ctcggcgatc tcttcgatag tcggagacct
      601 tcccagagtg tgcgtcaact cgtcgaccgc cctcgacacc tcgagccaga ggttctgcaa
      661 cgtccgcggg acacgaaccg accacgcctg atcccggaag tagtgcttga tctcaccgat
      721 gatcgtgcgg ccggcgaagg tggagaactg gatgccgcga tccgcgtcga agcgttcgat
      781 cgccttgaga agtccgaacc gggcaacctg gacgaggtcg tccaaaggga cgccacgacc
      841 accgaaacgt cgagcaagcc gttgcgccaa cccatcaaag gcctccacga gctcgcgttt
      901 ggcctcaggc tgctcgcgaa ctgcgaagag gtcctcgatc gcctcctgct cccgttcagt
      961 catcagaccc gggcgcctcg attgggatca gaacgaactc gccgacgacg gtggtgcctt
     1021 cgaaaagggc ctgggcgatg tctatcggcc cgaagtcctc gtctccgccc agcccgtcac
     1081 gccagggccc gatctccaga gtcaggccat ctggggaaag gtttgcccgg actgtgatga
     1141 aggaacgatc gcccgcggtg acgatggcgg tcgagatctc cgaggccgcc aacttggcgt
     1201 catcgatagt ctcgacgtcg agacccagcg atcgggcgac ggccgaaacg aacattcgca
     1261 ccgtggcgac atgatgcggg agactcgcca ccgtcagctc ataaacgcca ctcgacggcg
     1321 aaactggatt cgtcagcgct cctcccgaat gacttccgcc aacatctgga ggtcggcagg
     1381 cggcccgtag tatatggccc tgtggcgccc gaccagatca gactcgaagt tccggcgcgg
     1441 cgtcagtcac tgtttgtcct tcggaacatg gctggaaacg ccgcccgcct ctgcggcttc
     1501 agttacaacc gtgttgagga agcccggctc gcggtgaacg aagcagcgtc gctccttttg
     1561 gcagacggca ggtcttcgac ggtgcggtgc tccctcacct ccgacgaagg ggcccttttc
     1621 gtcgagatcg aggcaactcc gggcccggcg atatggccac cggagccctg ggccgactct
     1681 ctcgagcaca ccgtcctctc ctccctggcc aacgatctcg agctggtctc ggacgggggc
     1741 ccgatggtga ggatgacgtt gggggcacag tcccggggct aaatcgcctc gtctctgtca
     1801 aaacaaagac ttgcgtgccg gttccggtgc caataccttg agatcgttcg gtaactaacg
     1861 atcccaggtg ccctcttgac aggtgctctc tcgaagcgtc tctttcctct cggtcttgtg
     1921 gcggtcgttg gcctcatcac agcttgtggt tccggctcgt ctggcagcga cgaaacactc
     1981 gttatctact cagggcgggc cgaggaattg gtgtctccct tgtttgccag gttcaccgag
     2041 gagaccggaa tcggggtcga aatccggtat gggggctcga gcgaattggc agcgactctg
     2101 atcgccgaag gtgacgggac cgaggcagat gtcttcttcg cccaggaccc agcatccctg
     2161 ggcactgtgg actcgatgat gaaagttctc gacgacgcca ctctcgatct cgtggatccg
     2221 gctttcaacg accgcgacgg ccattgggtt ggcatttctg gtcgatcccg cgtggtcgtc
     2281 tacaacacgg acacgaatgt cccgctcccc cagacgatcg acgacatgac cgacccggtt
     2341 tgggccaacc agctcgggat cgctcccacc aatgcgtcct tcctcgcctt cgtgtcggcg
     2401 atgatcctcg agcggggtga agacggcacg cgcacttggc tggaggccct cgccgccaat
     2461 catccggtcg aatacgagag caacgcccca atcgtcgaag ccactgacaa cggtgagctc
     2521 gagggaggcc tcgtgaacca ctattacctc ctccgcctcc gcgccgaagg cctcggcgag
     2581 cacgcggaga actactacat cccggctgga gacgtcggct cgctgatcat gccggccggc
     2641 gtcgggatca tgtcggcaac ggaccagcct gagcccgccc agcgtttcgt cgacttcttg
     2701 ctctcggaag agtcgcaggc attcttcgca accgacacct tcgagtatcc actgatcgat
     2761 ggcgtccccc agcccgatgg ggcgcctcca ctctcag
//
LOCUS       DBNHXU010000293         2661 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_498270, whole genome shotgun sequence.
ACCESSION   DBNHXU010000293 DBNHXU010000000
VERSION     DBNHXU010000293.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2661)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2661
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_498270"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..483
                     /locus_tag="AC161X_07395"
     CDS             <1..483
                     /locus_tag="AC161X_07395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989412.1"
                     /translation="LKYHKAELCRPDKVVALQRGGEMEPVVGMLRRFLGLDVTVAPSD
                     PNLNPVSPDERAQRRTALFAAALAPPLDRWKVRPTVFAPTLPMGLNLGRLHHVLVGVQ
                     DEGGGCLGLGILIHEDDTLLVMTNRGEGMKGLRLGSMKLDPDSLAVTVISLREVMFGI
                     "
     gene            574..1227
                     /locus_tag="AC161X_07400"
     CDS             574..1227
                     /locus_tag="AC161X_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HIR4989413.1"
                     /translation="MLVAALATIGLGVAAGADSGSEGEFLAKINASRANAGLAPLAGK
                     GSLTSYARSHTAGMMDAGKIYHSTSAQLGAAGGTGWDRMGENVGRGQTPSSLHEAFMN
                     SSGHRANILGDYNYVGIGTGTKDGYLYVTVIFMKKGSSSAPATTTTTAADSPGTTEGS
                     NSGTTKPKPTPTTEVTSTTTTLPPTTTTTTMIVGPDKAVVPGEACVEAGRFGQLCHD"
     gene            complement(1494..1913)
                     /locus_tag="AC161X_07405"
     CDS             complement(1494..1913)
                     /locus_tag="AC161X_07405"
                     /inference="COORDINATES: protein motif:HMM:NF022182.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HIR4989414.1"
                     /translation="MREILEARIKLWLEDPESSVEYAEELEGRWAVRMRQQVRDATTV
                     WFTPLERSLEYEAYVIPVPVDAIEVMRQALVRNQRGWRAFFALDQEGDLLLRGRVAAE
                     AVTLLELDRVLGEIYETVELSFRAMVSVIAASRENSG"
     gene            complement(1910..>2661)
                     /locus_tag="AC161X_07410"
     CDS             complement(1910..>2661)
                     /locus_tag="AC161X_07410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4989415.1"
                     /translation="RIATEQEVIDESDVSVVSSPDESEELISRYGAEAEQISIVTPGV
                     DHDQFVPGVREAARIRLGWPDVETLLFVGRIHPVKGSDIAVRAFAEIATVRPEARLVL
                     VGAPAGDDGAREMEQLQRMVKRIDLGHAVTFAAPVPHQQLADVYRAADLVLVPSRSES
                     FGLVAAEAQASGLPVVAAAVGGLKTVVAEGSGGILVNGWDPGRWSDEALRVLVEPGLR
                     STLQAAGPRWAERFSWEAAVAGLGTIYGSLS"
BASE COUNT          515 a          914 c          809 g          423 t
ORIGIN      
        1 ctgaaatacc acaaggccga gctctgtcgc cctgacaagg tcgtggccct gcagaggggt
       61 ggggagatgg agccggtcgt gggaatgctg cggcgcttcc tcggcctgga cgtgaccgtc
      121 gcccccagcg acccgaatct caacccggtg tcgcccgacg agcgggcaca gcggagaact
      181 gcgctcttcg ccgccgccct cgcaccaccg ttggatcggt ggaaggtcag acccacggtg
      241 tttgccccaa ccctcccgat gggtctcaac ctggggaggc tccatcacgt cctggtcggt
      301 gtccaggacg aaggcggggg gtgtctcggg ctcgggatac tgatccatga ggacgacact
      361 ctgctggtca tgaccaatcg cggcgagggc atgaaagggc tccggctggg atcgatgaag
      421 ctggatcccg acagcctggc cgtcactgtg atcagtctcc gtgaggtgat gttcgggatc
      481 tgaccgtccc cagggcgttc tcgggggtta aaggcgaaag ggcctgtcgc cgataacccc
      541 tcaatgactg ggaaaaggcg ttggctatca ctattgctcg tcgcggccct ggccaccatc
      601 ggccttggcg tggcagcggg ggccgactca gggagcgaag gcgagtttct ggcgaagatc
      661 aacgcttcgc gtgcaaacgc aggtctggcg ccgttggccg gcaagggcag cctcacctcc
      721 tacgcgagga gtcacaccgc cgggatgatg gatgcgggga agatctacca ctcgacgagc
      781 gcacaactgg gcgccgcggg tggcaccgga tgggacagga tgggcgagaa cgtcggccgg
      841 ggccagaccc cgagcagcct ccacgaggcg ttcatgaact cctcggggca ccgagccaac
      901 atcctcggtg attacaacta cgtagggatc ggcaccggga ccaaggacgg ctatctctac
      961 gtcaccgtca tcttcatgaa gaagggctcg agctcggcgc ccgccaccac cacgaccacc
     1021 gccgccgaca gcccgggcac caccgagggc tcgaactcgg ggaccaccaa gcccaagccg
     1081 acgccaacga ccgaggtgac ctccaccacg accactttgc ccccgaccac caccaccacg
     1141 acgatgatcg tcgggccgga taaggcagtg gttcccggtg aggcctgcgt cgaggccggt
     1201 cggttcggcc agctctgcca cgactgacgg gcacacgaaa cgcccgagtg acctctcgga
     1261 gcctcctccg cacttccccg aacggacgct tctccgaact gcctctcggg cgttcgcagg
     1321 gctgaatcta cggattcgca cccgacggat caacaaccct ctgccggccc ccaaccatcg
     1381 tcctagagcg caacatatcg tcgttccggt gcgactccgg acctgacgaa cggttttcaa
     1441 cagcaaatgg ggttatccac agggcttgtc aacacgggta tgtttacaag tttctaaccc
     1501 gaattttcgc gactcgccgc gatcacactc accatcgccc ggaacgacag ctcgaccgtc
     1561 tcgtagatct ctcccagtac ccgatcgagc tcgagcaggg tcacggcctc cgccgcgacc
     1621 cgcccccgaa gcaagagatc accctcttgg tcgagggcaa agaaggcccg ccaccctcgc
     1681 tggttgcgga ccagggcctg gcgcatcacc tcgattgcat cgaccggcac ggggatcaca
     1741 taggcctcgt actcgagact tcgctccagc ggtgtgaacc agacggtggt cgcgtcgcgc
     1801 acttgttgac gcatccgaac cgcccagcgc ccctccaact cctcggcgta ctcgacactc
     1861 gactccgggt cttcgagcca caacttgatt cgggcctcga ggatctccct cacgacagag
     1921 atccgtagat ggtgccgagc ccggcgaccg ctgcctccca tgagaagcgc tcggcccaac
     1981 gcgggcctgc agcctgcaac gtcgagcgca ggcctggctc gaccaagact cgcaacgctt
     2041 cgtccgacca tcgacccgga tcccaaccat tgaccaggat tccgccagaa ccctcagcaa
     2101 ccaccgtctt cagaccgccg acagcggcgg cgaccaccgg cagtccggat gcctgggctt
     2161 ctgccgccac caggccgaag gactcgctcc gcgatgggac caggacaaga tcggcagccc
     2221 gatagacatc agccagctgc tgatggggta ccggcgcggc gaaggtgacg gcgtgcccca
     2281 ggtcgatgcg cttgaccatg cgctgcagct gttccatctc acgcgcccca tcatcgccgg
     2341 caggggcgcc caccaacacc aggcgagcct ccgggcgcac ggtggcgatc tctgcgaaag
     2401 cccggactgc gatgtcggat cccttgaccg gatggatacg gcccacgaag agcaaggtct
     2461 cgacatcggg ccaaccgagt cggatgcgag ccgcttcgcg gacgccagga acgaactgat
     2521 cgtgatcgac cccaggagtg acaatggaga tctgctcggc ttcggcgccg taccgggaga
     2581 tgagctcttc gctctcgtca gggctggaca ccacgctgac atctgactcg tcgatcacct
     2641 cctgctcggt ggcgatccgc a
//
LOCUS       DBNHXU010000294         3613 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4070612, whole genome shotgun sequence.
ACCESSION   DBNHXU010000294 DBNHXU010000000
VERSION     DBNHXU010000294.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4070612"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="AC161X_07415"
     CDS             <1..780
                     /locus_tag="AC161X_07415"
                     /inference="COORDINATES: protein motif:HMM:NF016027.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4989416.1"
                     /translation="SDLHIAVTRPAVARNAVEIVGADRVSEIRDVYPQAGYLAAFDLA
                     VSSAGYNAVHELIPAGVPSLFVANTSTRTDDQEARARSIEGLGLGLQARDNKPQEVEP
                     ALRRLLDRSTRKELAARGIATRSTINGAAETAALTLSIGDGFTRRRRSPRVVVSQQVQ
                     VGKESLKGALGEERTNSLKRLMGREPSPIGAKTKVRVVQTPQPSTGLDPLPLAVTESL
                     RREDLDLGTPIEHLLPGSSAAYREKRMEIIATYYELDKGAS"
     gene            complement(777..1850)
                     /locus_tag="AC161X_07420"
     CDS             complement(777..1850)
                     /locus_tag="AC161X_07420"
                     /inference="COORDINATES: protein motif:HMM:NF013700.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HIR4989417.1"
                     /translation="MRRLIVLVSALVTLVAFAAPAVAATFDPTQIALPLDGTHRITDS
                     FGDCRGTNCSRRHEGVDIMAAKGIPVLAAADGVVDWVPTACCGLGIDHGNGWQTRYIH
                     LNNDTQNPDGSYSDDGKAWGIAPGIVDGTAVTKGQVIGWVGDSGNAEAGSSHLHFEIR
                     QNGVAIDPYPYLIKAEGNWVGQFNDDETSVHQANIDKIFVAGITVGCNPPLNTKFCPQ
                     RNITRGEMAAFIARALHLTQMTGTPAYDDVDGHLFEIAIDKIQMAGIGFGCDENSFCP
                     DRPLERDEMAELLVRAFQYENPDATDFFTDDSGNQFETSINKLANAGITVGCNPPAND
                     NFCPDRQLSRGEMATFFVRALGL"
     gene            1967..3214
                     /locus_tag="AC161X_07425"
     CDS             1967..3214
                     /locus_tag="AC161X_07425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HIR4989418.1"
                     /translation="MPSLSRPRLFIALFAAIWAGVLVTSWEDLDPWLRGATVVVGVFL
                     TAVAVLRERPAAGNGGADPRLALIEQVVADETRRAWNPTTLPWTRTATSPKPVLLVPA
                     ADYHLAEVTPLARELERRGIPTRVAIGQSPWTRTWQGLASEDLEVFELPNPDDIADQV
                     SAVLVMKDTGSLGPWVDRCRELGLATIGKVEGAQDFWDADTPEERRPYRNLDLVLCQG
                     QFDAESLAGSDTEIVGSTRLERLWWAPPVAVPDPLALINLNFVYGVRTADRKLWLDTA
                     VQGCDLAGIPYVISTHPAEKSRIDSGRVTSISASRLLPHTSVLISRFSTMPLEAMARG
                     VPFVYHNPHGETVRTFADPMEGFHITNSADGLARAIKEANSSQAEYRSRIEPFFAAHI
                     DIDPARRSEVRSADVVSRLVKSS"
     gene            3211..>3613
                     /locus_tag="AC161X_07430"
     CDS             3211..>3613
                     /locus_tag="AC161X_07430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989419.1"
                     /translation="MRASQWLAARRGRVALLTLMAVGWTGVVLGAAAGQEFVVALGAG
                     VVAVANLVIAKAVLETRSSVDTLNRTAARSERAVTHLKAGVDRLRARTGALEARVASM
                     EPKINLLRAETPVTKDKITALRAESASLSGEI"
BASE COUNT          686 a         1167 c         1100 g          660 t
ORIGIN      
        1 agtgacctcc acatcgcagt gaccaggccg gcggtggccc gcaacgctgt cgagatcgtg
       61 ggagccgacc gtgtgagcga gatccgtgat gtctatcccc aggccggata cctcgccgcc
      121 ttcgacctgg ccgtcagctc cgccggatac aacgccgtcc acgagttgat acccgccgga
      181 gtgccgtcgc tgttcgtggc caacacctca acccgaaccg acgaccagga agcaagggcc
      241 cgcagtatcg aaggattggg cctcggcctt caggcccgtg acaacaagcc ccaagaagtc
      301 gaacctgcgc ttcggcggtt gctggaccgc agcacccgca aggagttggc tgcccggggc
      361 atcgccacgc ggtccaccat caacggcgcg gcagaaaccg cagccctgac cttgagtatt
      421 ggcgacggct tcaccagacg gcgccgaagc ccgcgagtgg tcgtctccca gcaagtccag
      481 gttggcaagg aatcgctgaa aggcgctctc ggagaggaac gaaccaactc tctgaagagg
      541 ttgatgggaa gggagccctc cccaatcggg gccaagacca aagtgcgcgt ggtgcagacc
      601 ccacagcctt cgaccggtct ggatccgttg ccgctcgccg tcaccgagtc gttgcgaaga
      661 gaggacctcg atctgggaac tccgatagaa catcttctcc cgggatcgag tgccgcctac
      721 cgggagaagc ggatggaaat catcgccacc tactacgagc tggacaaggg ggcttcctag
      781 agcccgagcg ctctcacaaa gaaggtggcc atctcaccgc gggagagctg acggtcaggg
      841 cagaagttgt cgtttgcggg cgggttgcat ccgacggtta tcccggcatt cgccagcttg
      901 ttgatcgaag tctcgaactg gttgccactg tcatcagtga agaagtctgt cgcatccggg
      961 ttctcgtact ggaaagcgcg caccaacagc tcggccatct cgtcacgttc caagggccga
     1021 tccgggcaga agctgttctc gtcacatcca aatccgatcc cggccatctg aatcttgtcg
     1081 atggcgatct cgaacaggtg accatcgacg tcgtcgtacg caggggtacc tgtcatctgt
     1141 gtgaggtgga gtgccctggc gatgaaggct gccatctcgc cgcgagtgat attgcgctgt
     1201 gggcagaact tggtgttcag cggcgggttg cacccgactg tgatcccggc cacaaagatc
     1261 ttgtcgatgt tcgcctgatg gaccgaggtc tcatcatcgt tgaattgacc gacccagttc
     1321 ccttcagcct tgatcaggta ggggtaggga tcgatggcga cgccattctg tcgaatctcg
     1381 aagtggaggt gagaagagcc agcttcggcg ttgcccgaat cccccaccca gccaatcacc
     1441 tggcccttgg tgaccgctgt cccgtcgacg atcccgggag cgatccccca tgccttgcca
     1501 tcgtcgctgt aggagccgtc ggggttctgc gtgtcgttgt tgaggtggat gtatcgggtt
     1561 tgccacccat tcccgtggtc gatgccgagg ccacaacacg cggtggggac ccagtcgacc
     1621 acaccatcgg ccgcggccag aacggggatt cccttggcag ccatgatgtc gacaccttca
     1681 tgacgccggg agcaattggt ccctcgacag tcaccgaagg agtccgtgat gcggtgggtg
     1741 ccgtccagag gcaaggcgat ttgggtcgga tcgaaagtgg ccgccacggc aggcgcagca
     1801 aaggccacaa gtgtgaccaa ggctgacacc agcacaatca gacgacgcac aaattcacca
     1861 aacttgaacc tcctatcgga cgcctacccg aatcacttga attccaaacc aagctgttgg
     1921 atccggttca gccaccctga ggcgggttac cgtttcctcg ttcgccatgc catctctcag
     1981 tcgaccccgc ctattcatcg cccttttcgc ggcaatctgg gccggcgtcc tggtcacctc
     2041 ctgggaggac ctcgaccctt ggcttcgcgg agcaaccgtg gtcgtgggag tcttcttgac
     2101 ggccgttgcc gtcctcaggg agcgtcctgc tgccggaaac ggcggcgccg atccacgcct
     2161 agccctgatc gagcaggtag tggccgacga aacacgccga gcatggaacc caaccaccct
     2221 cccttggacc cggacagcga cgtcgccgaa acctgtgctc ctcgtgcccg ctgccgacta
     2281 ccacctggcc gaggtgacac ccctcgccag ggagctggag cgccgaggca ttccgacccg
     2341 ggtcgccatt ggccagtcgc cttggactcg cacctggcag ggcctggcgt cagaggacct
     2401 cgaggtgttc gagcttccca accccgacga catcgccgat caggtctccg cggttctggt
     2461 gatgaaggac accgggagcc ttggcccttg ggtcgatcgc tgccgggagc tcggcctggc
     2521 gacgatcggc aaggtggagg gtgcccagga cttttgggac gccgacactc ccgaggagcg
     2581 acggccctat cgaaacctcg acctggtctt gtgccagggc cagttcgacg ccgaatctct
     2641 cgccggttct gacaccgaga tcgtgggaag cacgaggctc gagcgccttt ggtgggctcc
     2701 tccggtcgca gtccccgacc ctttggcgct gatcaatctg aacttcgtgt acggggtgcg
     2761 aaccgccgac cgcaagctct ggctcgacac tgcggtccaa ggctgcgacc tggcagggat
     2821 cccctacgtg atctcgaccc atccggcgga gaagtcccga atcgactccg gccgggtcac
     2881 ctcgatctcg gcttcgcgcc ttcttcccca cacctcggtg ctcattagcc gcttcagcac
     2941 aatgcccctc gaggcaatgg cccgaggagt gcctttcgtc tatcacaacc cgcatggcga
     3001 gacggtccgg acattcgccg atccgatgga aggcttccac atcaccaact ccgccgatgg
     3061 cctcgcccgt gccatcaagg aagccaactc atcacaggcc gagtaccgat cgagaatcga
     3121 gccatttttc gcagcccaca tcgacatcga tccagcaagg cgaagcgagg tgagatcagc
     3181 ggacgtcgtt agtcggttgg tcaagtcctc atgagggcca gccaatggct cgccgctcgc
     3241 cgggggcggg tcgccttgct caccttgatg gccgtcggct ggaccggggt cgtgcttggt
     3301 gcggcggccg ggcaagagtt cgtcgtcgca ctcggggctg gagtcgtagc ggtcgccaac
     3361 ctggtgatcg caaaggccgt gttggagaca aggtcgtcgg tggacaccct caacaggacc
     3421 gccgcacgat ctgaaagggc tgtcactcac ctcaaagccg gtgtcgaccg tctccgcgcc
     3481 cggaccgggg cattggaggc aagggttgcc tcgatggagc ccaagatcaa tctccttcgc
     3541 gccgagacac cggtgaccaa ggacaagatc accgcgcttc gcgccgaatc cgcttcgctc
     3601 tccggtgaaa tcg
//
LOCUS       DBNHXU010000295         4120 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4269983, whole genome shotgun sequence.
ACCESSION   DBNHXU010000295 DBNHXU010000000
VERSION     DBNHXU010000295.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4120)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4120)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4120
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4269983"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..841)
                     /locus_tag="AC161X_07435"
     CDS             complement(<1..841)
                     /locus_tag="AC161X_07435"
                     /inference="COORDINATES: protein motif:HMM:NF024110.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989420.1"
                     /translation="MSDLGVAWQVARRELTERGRSKSYLLTTLITLLVVVALIILPSL
                     FGGGTDEYDVGSVGEGNSEIIEAGELLTNANDEPDAEPTASITEVEFSTRDEGEAALE
                     AGEVDAVLVDGSEVVVARLGGFGESPIVTILQQGAASVRVEELVESEGDIASDVIEIM
                     ASEPLETSTLSGQADDDGSAGAIAYFSLLLLYLAILIYGQWILSAVTEEKSNRVVEVL
                     LSTVKPWQLLAGKIAGVGLLGLAQFAGTIIVAFLTLRVTGAYDIPPVDMTLVAHLVLW
                     FTLG"
     gene            complement(838..1725)
                     /locus_tag="AC161X_07440"
     CDS             complement(838..1725)
                     /locus_tag="AC161X_07440"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011873118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989421.1"
                     /translation="MLSIQDLHKTFGDVVALDGCTLSVQRGTMLGFLGPNGAGKTTAM
                     RAVFGLMRPDSGSVTWDGQPIDPDARLRFGYMPEQRGLYPKMRIHDQLVYLGRLRGIG
                     KAQAAESADKWLEILDLTERRDDRLDALSHGNQQRVQMAAALVHDPELLVLDEPFSGL
                     DPIASETMSDILRAQAASGKAVVFSSHQLDLVEDLCEEVAIIDKGTIVVEGSVRELKD
                     QAPIRHLELELDGDASSLFDSLDGVRNMEVEGERYSLVIDAGTDVRGLLNQAESVGRI
                     RHFTYTAPSLTDLFREAVQ"
     gene            complement(1815..2816)
                     /locus_tag="AC161X_07445"
     CDS             complement(1815..2816)
                     /locus_tag="AC161X_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014740611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HIR4989422.1"
                     /translation="MGGLLRRTRVEREQLERDLLAPIASLSSETAGRAEPEEDDEFRT
                     AFERDRDRVIHSKAFRRLKHKTQVFLNPDGDHYVTRMTHTIHVTQVGRAMARALGLNE
                     DLTEAICLAHDVGHSPFGHIGEDALSPFVDGGWLHSEHGVRTLELLEPQNLTSEVLDG
                     VRAHSWKIDPPPATLEGWLCRYADRIAYLTHDVADALRAGILEYHEIPTQALARLGAT
                     SREWIDTMVTAVIEASYLAGEVTIDSDLLQVMDELRDFMFDRVYLRPESEGQKQKAIG
                     VIEDLVVHFSNNPGEVPDSFTTPTADALHRAIDYVAGMTDRFALNTHDRLFRPTLLD"
     gene            complement(2840..>4120)
                     /locus_tag="AC161X_07450"
     CDS             complement(2840..>4120)
                     /locus_tag="AC161X_07450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989423.1"
                     /translation="VAASFIAPSTVIPLLLLGANAAALGAGWWMIIPASLAFLGLLAV
                     GGQLFSAAISAILRTRRGRDIATFLILGIAGGAFLFYRFVNELVTTEGITAAATTHPI
                     MNWSLLIPPVAAQQAMIRAADGSAASALVSLAAAVAGLLIFAVAWKKLLAWLLSTPEQ
                     RNKPAARTRRGGMAYGPWGVVPTLARKEFRFYVRDPRQRLVWTGTVIFVGLAIGGVVM
                     NATGFFDFREREWAPLVAPILVLFVGLPIALNLFGWERNAASYLFVLPARPRQLLLGK
                     NMAVATALALETAVLTLGLAWFSGHWRWVWLTIPLTVAAVGCQLAVGNLVSVLAPLRL
                     PREGTDVFAQSTEQGCLAIVSQTVSFFAIGLLLIPPASVTVLTVEFGEVINPWITAVL
                     GIAWGLLFYSASLLLAGKLLKRRMPEVLAWVQVT"
BASE COUNT          816 a         1264 c         1336 g          704 t
ORIGIN      
        1 aaccgagagt gaaccagagc acgaggtggg cgaccaaagt catgtccacc ggaggaatgt
       61 cataggcgcc cgtgacccgc agcgtgagga aggcaacgat gatggttccg gcgaactggg
      121 ccagcccaag gaggccgacc ccggcgatct tccccgcaag cagctgccac ggcttgacgg
      181 tggacaggag gacttccacc acccggttcg acttctcttc ggtgactgct gaaaggatcc
      241 attggccgta gatgaggatc gccagataga ggagcagtaa tgagaaatag gcgatggcgc
      301 ccgctgagcc gtcgtcgtcg gcctgaccac tcagagtcga ggtctcaagt ggctccgaag
      361 ccatgatctc gatgacgtca gaggcgatat ctccctcgct ctctaccagt tcctcgaccc
      421 ggacagaggc cgctccctgt tgcaggatgg tcacgatcgg tgattctcca aagcctccca
      481 gccttgcaac gactacttcc gacccgtcga cgagcacagc atcgacttct ccagcctcga
      541 gggccgcttc gccctcatcg cgggtcgaga actcgacttc ggtgatactg gccgtgggct
      601 cggcatcggg ctcgtcgttg gcgttggtta ggagctcccc ggcctcgatg atctcgctgt
      661 tcccttcgcc gacggacccg acgtcgtatt cgtctgtgcc cccaccgaag aggctgggca
      721 ggatgatcag cgccaccacg accaggagag ttatcagagt cgtgagcaga taggacttgg
      781 agcgaccgcg ttcggtgagc tcgcggcggg ctacctgcca ggcgactccg agatcgctca
      841 ttgcaccgcc tccctgaaga ggtcggtcaa cgatggcgcg gtataggtga agtggcggat
      901 ccgtcccacg ctttcggcct ggttcaacaa acctctcacg tcggtcccgg catcgatcac
      961 caggctgtaa cgctcgccct ccacctccat gttgcggacg ccgtcgagtg agtcgaagag
     1021 gctgctggca tccccgtcga gctcgagctc gagatgccgg atcggcgcct gatccttcag
     1081 ctcacgcacc gatccctcga cgacgatcgt tcccttgtcg atgatcgcga cctcctcaca
     1141 aaggtcctcg accaggtcga gttgatggga ggagaaaacc acagctttac ccgaagccgc
     1201 ctgcgccctc aggatgtcgg acatcgtctc agaggcgatg ggatcgagtc ccgagaaggg
     1261 ctcgtcgagc accaacagct ctgggtcgtg gaccaaggcc gccgccatct gcacccgttg
     1321 ctgatttccg tgggacagag catcgagccg gtcgtcccgg cgctcggtga ggtcaaggat
     1381 ctcaagccat ttatcggcgg actcggcggc ctgggccttc ccgatcccac gaaggcggcc
     1441 caggtacacc agttggtcgt ggatgcgcat cttcgggtag aggccccgct gctcgggcat
     1501 gtaaccgaag cgaagccggg catcgggatc gatcggctgg ccgtcccagg tgaccgatcc
     1561 cgagtcgggc cgcatcagac cgaaaacggc gcgcatcgcg gtcgtctttc ccgctccgtt
     1621 ggggccgaga aagccgagca tcgtcccccg ctgcaccgaa agggtgcatc catcgagcgc
     1681 gaccacgtcg ccgaaggtct tgtgtaggtc ctggatggag agcacgggtt ggggaggtta
     1741 ggccgcccgt aattgagaca gaaacgggtc acattcggac ctttggctcc caattacacg
     1801 ggaggcggga agtttcagtc gagcaaggtg ggtcggaaga gccggtcgtg ggtattgaga
     1861 gcgaacctgt cggtcatgcc ggcgacgtag tcgatagccc tgtgcaaggc gtctgctgtg
     1921 ggggtcgtga atgagtcggg aacctcgcct ggattattgg agaagtgcac gaccagatcc
     1981 tcgatgacgc ctatcgcctt ctgcttctgg ccctcggact caggccgtag atatacccga
     2041 tcgaacatga aatcgcggag ctcgtccatg acctgcagca gatcggaatc gatcgtcact
     2101 tcgccggcca ggtaactggc ttcgatgaca gcggtcacca tcgtgtcgat ccattccctc
     2161 gacgtggcac cgagccgcgc cagagcctgg gttgggatct cgtggtactc gaggatcccg
     2221 gcgcggaggg cgtccgcgac gtcgtgggtg aggtaggcga tgcgatcggc atagcggcac
     2281 agccaccctt cgagcgtcgc gggtgggggg tcgattttcc aggagtgggc ccggacacca
     2341 tccaggactt cggaggtgag gttctgaggc tcgagcaact cgagagtccg cactccgtgc
     2401 tcggagtgga gccacccgcc gtcgacaaat ggtgacagtg cgtcctcccc gatatggccg
     2461 aaaggtgaat ggccgacatc gtgggccaga cagatggctt cggtcagatc ctcgttgagg
     2521 ccgagggccc gggccattgc ccgacccacc tgggtgacgt ggattgtgtg ggtcatcctc
     2581 gtgacgtagt ggtcaccatc gggattgagg aacacctgag tcttatgctt gaggcgtcgg
     2641 aacgccttgc tgtgaatgac ccggtcccga tcacgctcga atgcggtccg gaactcatcg
     2701 tcctcttcag gctcggccct ccccgctgtc tccgaggaga gggaggcgat gggcgccagc
     2761 aagtcgcgct cgagttgctc gcgttcgacg cgggtccgtc gcaagagtcc gcccatcggg
     2821 aagccagcgt agggacccgc taggtgacct ggacccaggc cagcacttct ggcatccgcc
     2881 gcttcagcaa ctttccggcc agtaacaagg aagccgagta gaacaacaac ccccaggcga
     2941 taccgagcac agccgtgatc cacggattga tgacctcacc gaactcgaca gtcagcacgg
     3001 tcaccgaagc tggagggatc agcaggagtc cgattgcgaa gaacgacacg gtctggctga
     3061 cgatcgccag gcagccctgc tcggttgatt gggcaaagac gtcggtcccc tctcggggca
     3121 gacgcagtgg ggcgagaact gacaccagat tgcctaccgc gagctgacat ccgacagcag
     3181 ccaccgtcag gggaattgtg agccaaaccc agcgccaatg gccggagaac caggcaagcc
     3241 ccagagtgag gaccgcggtc tccagagcga gagccgttgc cacggccatg ttcttgccaa
     3301 gcaacagctg gcggggacgg gcagggagca cgaagaggta gctggccgcg ttccgctccc
     3361 agccgaacag gttcagggca atcggaagac cgacgaagag cacgaggatg ggagccacca
     3421 acggtgccca ctcccgctct cgaaagtcga agaacccggt ggcgttcatc acgacccccc
     3481 cgatggcgag gccgacgaag atcacggtgc cggtccatac cagccgctga cggggatcac
     3541 ggacatagaa gcggaactcc ttccgggcga gcgtcggcac cacgccccac gggccgtagg
     3601 ccatgccccc gcgccgtgtc ctcgctgccg gcttgttccg ttgttctggt gtggatagaa
     3661 gccaggcgag aagcttcttc cacgccaccg caaagatgag gaggcccgcg actgccgcag
     3721 ccagggagac gagggctgag gcggcgctgc catcggccgc ccggatcatg gcttgctgag
     3781 ccgcgaccgg tgggatcagg agtgaccagt tcatgatcgg gtgggtggtt gccgccgcgg
     3841 ttatcccttc ggtggtcacc aattcgttga cgaaccggta gaagaggaag gcaccgccgg
     3901 cgatgccgag aatcaggaag gtggcgatat cgcggccgcg tcgggttcgc aatatcgccg
     3961 aaatcgcggc agagaaaagt tgaccgccca cggccagcag accgaggaag gccaacgatg
     4021 ccgggatgat catccaccac ccggcgccga gtgccgcggc gttggcgcca aggagaagaa
     4081 gcgggatcac cgtggaaggt gcgatgaagc tggcggccac
//
LOCUS       DBNHXU010000296         2169 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2882032, whole genome shotgun sequence.
ACCESSION   DBNHXU010000296 DBNHXU010000000
VERSION     DBNHXU010000296.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2882032"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1442)
                     /locus_tag="AC161X_07455"
     CDS             complement(<1..1442)
                     /locus_tag="AC161X_07455"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4989424.1"
                     /translation="MTSAPGLGGNARDRKAALITGLAGGLIGFFVALGPLLSGDPAGT
                     IRAVASALIAAVSLYELMNKQRTEVVAALAAIIFALLSLFEASGSMKVIGFDTGTTLS
                     FMILAAIVYVTTRRSKATTPLIVGSTLIALFTVASVAIRRPEFSTAISWLLIGIPGQI
                     LVIWLTWRLIHSLAVASELEATHSRIQQALARCSQVLLTRRDDEPLTAALQALLGSTE
                     ADYVYVDMNRVDRKGHVTWEIVAEAYGPNIPPGDNLFDSGDYEQLQDVEKALFAGEPA
                     YVIVSEMPRPIRDRYEAEGIMAELMAPIFIRDQWVGTIGYSDFWREGTWLPAEVEGLT
                     RAADMVGAYWEREHAREGLEELAEAKDRFIATVSHELRTPLSAVVGFAGELAEGVENY
                     STEEIAEMVHLIAGQSVEMAQLVDDLLTAERAASGNLTIKPTAIDLLHETRSVVDSMR
                     VGENIVVDGDPTPAWADTLRTRQIVRNLLT"
     gene            1600..>2169
                     /locus_tag="AC161X_07460"
     CDS             1600..>2169
                     /locus_tag="AC161X_07460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR4989425.1"
                     /translation="MASVMVLILAACTGDNAQTPSPAASIDLGTSTTTSVAITEVPTT
                     QRSAPSGAERLVVVNGEGNIFTLDPDGSNPTQLTNKVPTARYFQPVWSPVGDRLVWGE
                     ASGPGFGVGSSAGDGSDAHLVPTSSLPFYLSWSADGSQVGILYGGAQGVVLEVVDMES
                     NTASPIGSGSPFYFSWDPQSTNMAVHVGAN"
BASE COUNT          380 a          674 c          693 g          422 t
ORIGIN      
        1 ttggtcaaca ggtttcgcac gatctgtcgg gtgcgaaggg tgtcggccca agcaggcgtg
       61 gggtcaccgt cgaccactat gttttcgccg actctcatcg agtcgaccac cgatctcgtc
      121 tcatgaagta ggtcgattgc tgttggcttg atcgtcaggt tgcccgaggc cgcccgctcc
      181 gcagtcagca ggtcgtcaac gagctgggcc atctcgacgc tctgacccgc gatgaggtgg
      241 accatctcgg cgatctcttc agtggagtag ttctcgaccc cttcggccag ctcgccggcg
      301 aagcccacca ccgcggacag cggtgtgcgc agttcatgac tgaccgtcgc gatgaatcga
      361 tccttcgcct ccgcgagctc ctcgaggccc tcccgggcgt gttcgcgttc ccagtaggcg
      421 cccaccatgt cggcggctcg ggtcagacct tcgacttcgg ccgggagcca ggtgccctct
      481 ctccagaagt cggagtatcc gatggtcccc acccactggt cgcggatgaa gatcggggcc
      541 atcaattcgg ccatgatccc ctcggcttcg tagcggtccc ggatcggcct cggcatctcc
      601 gacacaatta cgtaggcggg ctcccctgca aacagggcct tttccacgtc ctgcagttgt
      661 tcgtagtccc cgctgtcgaa gaggttgtct cctggcggga tattgggccc gtatgcctcg
      721 gcgacgatct cccaggtgac atggcccttg cgatcgaccc ggttcatgtc gacgtatacg
      781 tagtcggcct cggtggatcc gagaagcgcc tgcaaagcgg cggtcagtgg ctcgtcgtca
      841 cgacgggtga ggaggacctg ggagcatctc gccagcgcct gttggatgcg ggagtgggtg
      901 gcctccagtt ctgaggcaac agccagtgag tgaatcaggc gccaggtgag ccagatcacc
      961 aggatctggc cggggatccc gatgaggagc cagctgatcg ctgtggagaa ttcgggtcgt
     1021 cggattgcca ccgatgccac ggtgaataga gcgatgaggg tcgaccccac gatcagcgga
     1081 gtcgtggcct tcgaccgccg ggtcgttaca tagacaatcg ccgccaggat catgaacgag
     1141 agggtggtcc cggtgtcgaa gccgatgacc ttcatgctgc ccgaagcctc gaagaggctg
     1201 agtaaggcga agatgattgc agccaacgct gcgacgacct cggtgcgttg cttgttcatc
     1261 agctcataga gcgagaccgc ggcgatcagg gcggatgcga cggcccggat ggttccggcc
     1321 ggatccccgc tgagaagtgg gcccagggcc acgaagaagc caatcaggcc cccggcgaga
     1381 ccggttatga gtgcggcctt tcggtcgcgg gcgttgccac cgaggcccgg cgctgaagtc
     1441 attgctggtt tatcggacgt tctggcacgt gaattgatgt caaccccccg tcgcgcagcc
     1501 agtggtgccg aaaggcacac ggagggggta ccacctccgt gtgaccccat cgcagcagcc
     1561 agtacccttg aagccaggat gtcgaggcga gcgcttctga tggcctccgt catggtcttg
     1621 atcctcgccg cctgtaccgg cgacaacgcc cagaccccga gcccggccgc ctcgatcgac
     1681 ttggggacct caaccaccac gtcggtcgcc atcaccgagg tgcccacaac acagcggtcc
     1741 gcccccagcg gagccgagcg tctggtggtg gtgaacggcg agggaaacat cttcacactc
     1801 gaccctgatg gctccaaccc gactcaactg accaataagg tcccgactgc ccgatatttc
     1861 caaccggtct ggtcaccggt gggcgatcgc ctggtgtggg gtgaggccag tggccccggg
     1921 tttggggtgg gttctagtgc cggagatgga agtgacgccc atctggttcc gacttcatcg
     1981 ttgcccttct atttgagctg gtcggctgat gggtctcagg tcggcatcct ctatggaggg
     2041 gcgcagggtg tcgttctgga agtggtcgac atggagagca acacagcttc tcctatcggc
     2101 tccggctcgc ccttctactt ctcctgggat ccgcagagca cgaacatggc ggtccacgtc
     2161 ggagccaac
//
LOCUS       DBNHXU010000297         2004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4271037, whole genome shotgun sequence.
ACCESSION   DBNHXU010000297 DBNHXU010000000
VERSION     DBNHXU010000297.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4271037"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..279
                     /locus_tag="AC161X_07465"
     CDS             <1..279
                     /locus_tag="AC161X_07465"
                     /inference="COORDINATES: protein motif:HMM:NF014522.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007155 - cell adhesion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fasciclin domain-containing protein"
                     /protein_id="HIR4989426.1"
                     /translation="FAAIPEADLSAILADQDLLTSILTYHVVAGESLDAQELIDAGTV
                     ETVNGETLTIATSGDSVTINGVAMVVCGNVATANGTVHIVDAVLVPSA"
     gene            complement(356..1423)
                     /locus_tag="AC161X_07470"
     CDS             complement(356..1423)
                     /locus_tag="AC161X_07470"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989427.1"
                     /translation="MNSQGLDARLVVGRGDTFQLDVSLSIPPGRTVALLGPNGAGKST
                     AVAALAGLLPIDAGQITLGGVTLDDPDHGAFVSPDARKVGVVFQDYLLFPHLTVLENV
                     AFGLRSQSAGRAEAQARAVAWLEKMGLGGHLRVKPGGLSGGQAQRVALARALVTEPDL
                     LLLDEPLSALDVTTRVEMRRALAEHLESFPGPRLFITHDPTEAFLLADEIHIIEQGQV
                     TQAGNADDIRLRPRTRYAADLAGSNLVTGTAVGGDVNTGSHVLHVADHEISGPVLATI
                     RPAAISVHLREPEGSPRNSWTTTVDLVEHLGERTRLRTGNPLALTIEVTEEAARSLAL
                     VEGKQVWISIKATEITVEPDS"
     gene            complement(1420..>2004)
                     /gene="modB"
                     /locus_tag="AC161X_07475"
     CDS             complement(1420..>2004)
                     /gene="modB"
                     /locus_tag="AC161X_07475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015579696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HIR4989428.1"
                     /translation="GIPLAWLMARIEFPGRALARGLVTLPLVLPPVVGGAALLFALGR
                     RGFIGEPLNQATGLLLPFSTWGVVIANTFVAMPFLVITVEAALRNLDQRYEGAAATLG
                     AGRWTVLRRVTLPMIAPSLIAGLVLTWARAFGEFGATITFAGNLQGRTQTLPLAVFVA
                     MESDRDTAVVLSLMMVIVSLAVLLALRERWWKAL"
BASE COUNT          413 a          635 c          625 g          331 t
ORIGIN      
        1 ttcgccgcca ttcccgaagc ggatctgagc gcgatcctcg cggatcagga tctactcacc
       61 tcgatcctca cctaccacgt ggtggccgga gagtccttgg acgcccagga gttgatcgac
      121 gccgggaccg tggaaactgt caacggagag acactcacca ttgccacctc gggagacagc
      181 gtcaccatca atggtgtggc catggtcgtc tgtggcaatg tcgctacggc gaacggaacc
      241 gtccatatcg tcgatgcggt gctggtcccc agcgcctgac ccatcgcttc atagtgccca
      301 aaaaaggggg gcggagactt cggtgtccgc ccccccttcc attggtcatg ccgggtcaac
      361 tatcgggctc aaccgtgatt tcggtcgcct tgatcgagat ccacacctgc ttgccttcga
      421 cgagagcgag ggagcgggcg gcctcttcgg tcacttcgat ggtgagagcg agagggttgc
      481 ccgtcctcag gcgagtccgc tcgccgaggt gctcgaccag gtcaacggtg gtagtccagg
      541 aattgcgcgg gcttccttcc ggctcgcgga gatgaacaga aatggcggca ggccggatcg
      601 ttgccaggac cgggccggag atctcatggt cggcaacatg aagtacgtgt gagccggtgt
      661 tgacatcgcc acccactgcg gtccccgtca ccagattgga tccggccagg tcggccgcat
      721 agcgagtccg gggacggagc cggatgtcgt cggcgtttcc ggcctgagtc acctgtccct
      781 gctcgatgat gtggatctcg tcagcgagga ggaaggcctc ggtcgggtca tgagtgatga
      841 agaggcgagg gccggggaaa ctctctagat gctcggccag ggcccgacgc atctcaaccc
      901 gggtcgtgac gtccaacgcg gacagaggtt cgtccagtaa cagcagatcg ggctcggtga
      961 ccagagcgcg ggccagtgcc actcgttgcg cctggccccc cgaaaggccg cccggcttca
     1021 cacggaggtg gccgcctagt cccattttct ccaaccatgc gacggctcgg gcctgagcct
     1081 cggctctgcc cgcgctctga cttcggagac cgaaagccac attctccagg accgtcaagt
     1141 ggggaaagag caggtagtcc tggaagacga cacccacctt gcgggcgtcc ggggaaacaa
     1201 aggccccgtg gtccggatca tccagggtga ctcctccgag agtgatctgc cccgcatcaa
     1261 taggcagcag cccggctaga gccgctacag ctgttgactt gccggcaccg ttgggtccaa
     1321 gcaaggccac tgtccgaccc ggagggatcg aaagagaaac gtcgagttgg aaagtatcgc
     1381 cacgacccac caccagcctt gcgtcgaggc cttgggagtt caaagcgcct tccaccaccg
     1441 ctcacgaagc gccagcagaa ccgccagaga gacgatcacc atcatcaagc tcagaactac
     1501 cgccgtgtca cggtcgcttt ccattgccac gaacacggcg aggggaagcg tctgagttcg
     1561 cccttgaaga tttccggcga aggtgatcgt cgccccgaac tcgccgaagg cacgagccca
     1621 ggtgaggacc aatccggcga tcagcgaggg ggcgatcatc ggaagggtca cccgtcgtaa
     1681 caccgtccac ctaccggccc cgagagttgc agccgctccc tcgtagcgtt gatcgaggtt
     1741 gcgcaacgcg gcttcgaccg tgatcaccag aaagggcatt gcgacaaacg tgttggcaat
     1801 caccacaccc caagtcgaga agggcagcaa gagccccgtc gcctgattca gtggttcacc
     1861 gataaaccca cgacgcccaa gagcgaaaag gagagcggct ccacctacca ccggcggcaa
     1921 gaccaagggg agtgtgacga ggccgcgggc cagggcgcga ccagggaact cgatcctcgc
     1981 catcaaccag gcaaggggta tccc
//
LOCUS       DBNHXU010000298         2009 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_899447, whole genome shotgun sequence.
ACCESSION   DBNHXU010000298 DBNHXU010000000
VERSION     DBNHXU010000298.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2009)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2009
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_899447"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="AC161X_07480"
     CDS             <1..714
                     /locus_tag="AC161X_07480"
                     /inference="COORDINATES: protein motif:HMM:TIGR03883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent metalloprotease"
                     /protein_id="HIR4989429.1"
                     /translation="EMTAVLGVLARRVLGQYELVLPTGEDGDVVAFVGPNIMQMERTH
                     QFRPSEFRFWVTLHEMTHRAQFQGIPWLREYFMGLVSELVEESVPEPGRLNRMMEEIT
                     TRRESGESLLDDRGLLGLFATSSQRAIIDKVQALMSLLEGHGHVVMDRLGHEHLKSQE
                     RMSRVLKNRKLDKRTAAFFRITGLEMKLNQYRMGEQFIKAVEREAGWDTVNLAFRGVG
                     SLPTLEEIEAPSRWLQRVA"
     gene            693..1940
                     /gene="tilS"
                     /locus_tag="AC161X_07485"
     CDS             693..1940
                     /gene="tilS"
                     /locus_tag="AC161X_07485"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HIR4989430.1"
                     /translation="MVATRRLNGLVGRLESLLELHPGRPLVALSGGADSAAMAYLVAQ
                     TSSDLKAVHVNHSLRDSSRLEAAATAIASKLDSPLQVVTVVVPHGSSPEGQARAVRYQ
                     ALVEASDPGEVILTGHTLDDQAETVLLNILRGTGTRGLTGIPGWRAPNISRPMLRITR
                     SETRELALLAGLPYFDDPMNSETSFTRNAIRLKVMPDLLRFNPQLMSGLARMADSVRA
                     DSDALDQEAAALPVIVEDHRAQVAVGALTGVERPIANRVLASMAGRFREHAGLGAEEI
                     ARVWRVIDGGSVMEELAGGLVVRHVGPMLRLEHLDVKVHQNSRVALGPGSHRVGDTVF
                     EVDLVDEVCQVAPLGTWGAIFRPDANLVATASGGDGVVVEVDGEPAWVPGLRRAPVAW
                     YQPASRGYLSVSAREESGWTSSP"
BASE COUNT          352 a          624 c          655 g          378 t
ORIGIN      
        1 gagatgactg cggttctcgg cgtcctcgct cgtcgggtgc tcggtcaata cgaactagta
       61 ctccccacgg gcgaagacgg cgacgtggtt gcgttcgtcg gtcccaacat catgcaaatg
      121 gagcgcaccc atcagttccg cccatccgag ttccggttct gggtgaccct tcatgagatg
      181 acccaccgtg cccagtttca ggggatcccc tggcttcgcg aatacttcat gggcctcgtt
      241 tccgagttgg ttgaggagtc ggtcccggag ccggggcgcc tcaaccggat gatggaggag
      301 atcaccaccc gtagagagag tggcgagtcg ttgttggacg accggggtct tctcgggctg
      361 ttcgccacct cgagccagcg cgccatcatc gacaaggtgc aggctctgat gtcccttctc
      421 gaaggtcacg gacatgtggt gatggaccgg ctggggcacg agcatctcaa gtcccaggaa
      481 cggatgagcc gggtcctcaa gaaccggaaa ctcgacaagc ggactgctgc gttcttccgg
      541 atcaccggtc tggagatgaa gctcaatcag taccggatgg gtgagcagtt catcaaggcc
      601 gtggagagag aggcaggttg ggacactgtc aacctcgcct ttaggggtgt gggcagtctt
      661 cccacgctcg aagagatcga ggctcccagc cgatggttgc aacgcgtcgc ctgaacgggc
      721 tcgtcggccg gctggagtca ctactcgaac tgcaccccgg tcgacccttg gtcgctctct
      781 cgggtggcgc cgactcggca gctatggcct acctcgtggc ccaaacctca tcggatctga
      841 aggctgtaca cgtcaaccat tcacttcgcg attcgagccg cctcgaggcg gcggccacag
      901 cgatcgcttc caagctcgac agccctctcc aggtggtcac agtggtcgtg cctcacggca
      961 gctcaccgga gggtcaggcc cgggcagttc gctaccaggc cctggtcgaa gcctcggatc
     1021 ccggtgaggt catcctcacc gggcacactc tcgacgacca ggcggagacg gtcctactca
     1081 acatcctgcg cgggaccggc acccggggtc tgaccgggat tcccgggtgg cgggctccga
     1141 acatcagccg gccgatgctc cgcatcacca gatcggagac ccgggagttg gcgctgctgg
     1201 ccgggcttcc gtacttcgac gacccgatga acagcgagac gagctttacc cgcaatgcga
     1261 tccggctcaa ggtgatgccc gacttgctcc gtttcaaccc gcagctgatg agcggcctcg
     1321 cccggatggc cgattcggtg agggcggatt ccgatgctct cgatcaggaa gcagccgctc
     1381 tgccggtgat cgtcgaggac catcgagccc aggtcgctgt cggagccttg accggggttg
     1441 agcgcccgat cgccaaccgg gttctggctt cgatggcggg caggtttcgg gaacacgccg
     1501 ggctcggcgc agaggaaatc gcccgggtct ggcgggtgat cgacggtgga tccgtgatgg
     1561 aagagctggc aggcggcttg gtagtccgac atgtcggccc gatgcttcgc ctcgagcatc
     1621 tcgatgtgaa ggttcaccag aattctcgtg tcgccctcgg accgggatcg catcgagtcg
     1681 gggacacggt cttcgaggtg gatctcgttg atgaggtgtg tcaggtcgcg ccactaggca
     1741 cctggggtgc gatcttccgc cctgacgcca atcttgtcgc caccgcatcc ggaggggatg
     1801 gcgtcgtggt cgaggtcgac ggggaaccgg cttgggtgcc cggactgcgc cgcgctccgg
     1861 tcgcctggta tcagccggcc agccgcggct acctttccgt ttccgccaga gaggaatccg
     1921 gatggacatc aagcccctga tcaccgccga tgacatcaac gccaagctcg ccgagatggg
     1981 caagcgcatc actgacgatt actcgggag
//
LOCUS       DBNHXU010000299         4251 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2885225, whole genome shotgun sequence.
ACCESSION   DBNHXU010000299 DBNHXU010000000
VERSION     DBNHXU010000299.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2885225"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(35..1288)
                     /locus_tag="AC161X_07490"
     CDS             complement(35..1288)
                     /locus_tag="AC161X_07490"
                     /EC_number="1.5.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF014774.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HIR4989431.1"
                     /translation="MGWVTAEHPTTDELNACVQCGLCLPHCPTFRLTGRETASPRGRL
                     HAMSAVHEGVVEVDAAFERIIDFCLGCRACEPVCPGLVPYGRTLEGTRAEIVAQRPSM
                     SHRLRGFLLGSVLGSRRSLRFGTGALSLGQRVRATKMAPRRWRRLAAGMRELQSRPGS
                     VVGHNGGEGRSGRVALLAGCIQDQWFQPVNHAAVELLEMAGYRVEVPGGQTCCGALAA
                     HDGKAPEAHRLAERNTPILSGYDMVVATAAGCSAHLKDYDHWADGGEGVGSTAMDVTV
                     AVARAIEQGLLPRAAVSLGPIGIQDPCHLRHAQKVTAEPRLILESAGYEVIEIDPTGM
                     CCGAAGLYTVLQPEASQELGNRKASQVRSTGVKKVASANPGCEMQLRSALGDGFEIAH
                     PIEWYLQAIKRENPGGIVAVLEESR"
     gene            complement(1288..2394)
                     /locus_tag="AC161X_07495"
     CDS             complement(1288..2394)
                     /locus_tag="AC161X_07495"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013714.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR4989432.1"
                     /translation="MVPGAIPATLRSMEAEIPPGSMAGTGLAPASPEEVSKVLRFATE
                     NRLKVQVWGGGSHQGYGRPEKPDLVLVTDRLDAVEAWEPDDLTLVLGSGTPVAKFEEM
                     LAGRSQTAVMPENPGPATIGGVISAGVSSLKRARLLGTRERLLEVTLVTGDGRIVRAG
                     GRVVKNVSGYDLSRFVVGAFGALGVVVSVCLKLWPMPPAKATVTNPDPDRLHLLSRPL
                     AVLESDGVTKVFLWGIDQEVEANARQIGGELTPGHVWPNDPEGEFKWSMRIPPAVTAA
                     ATSRLPEGWRYLAIHGAGEIRLGSDDAAGATALREWAEANAGGLVVVDSPAGSLPDFD
                     PWGAPPQGLDIQRRLIGQFDPSRVINPGRLPGGI"
     gene            complement(2397..3824)
                     /locus_tag="AC161X_07500"
     CDS             complement(2397..3824)
                     /locus_tag="AC161X_07500"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012641672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HIR4989433.1"
                     /translation="MSLISDLEQLLGADRVLHEPVELYLYSKDASSLVRGHPNCVAFP
                     RTTEEVAEIVKIAEANGAPVVARGAGTGLTAGASPTEGGVVVSTTSLNAVDIDVDNRA
                     AWVGAGVINLDLTRAAAPFGLQFAPDPSSQSACTVGGNVANNSGGPHCLAEGSTTSHI
                     LGLEVVLAGGEVMVLGGLAPDTPGLDLKGLIVGSEGTLGVVTRALVRLIPVDPDVRTL
                     LIDFDRVGDACGTVSDIIAAGCVPAALEMMDQRMMQAVENWLQAGLPTNRAAILLVEL
                     VGETAGVEAESALVQDIATRRNSQAIRVAATEAERQLLWKGRKSAFGAVAQLAPDYYL
                     HDTVVPRTRLVETMLEVDAIADRYGLLLVNVFHAGDGNLHPLIAFDSSDPIAAEKVRK
                     AGDEIVALCLRNGGALSGEHGIGIEKRDLMRLNFSELDLDAQARLKKVFDPAATFNPS
                     KVLPQGSLCFDFGGVRKEIPEGAWV"
     gene            complement(3870..>4251)
                     /locus_tag="AC161X_07505"
     CDS             complement(3870..>4251)
                     /locus_tag="AC161X_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989434.1"
                     /translation="LWRVKTIVRQAFTVAFLALLLIPVFYMTEDLESTIRVGSAALVI
                     SILIDVWINRHPDPTIWVPRVSWTIAMVFSGIYAAIQLFNLSLASLGLLQLGFLLELA
                     SPAGIFSNFVRELGHSGRPETEAE"
BASE COUNT          818 a         1402 c         1342 g          689 t
ORIGIN      
        1 atggcgaggg gatcggtgcg gctggcgctc tcggtcaacg tgactcctcc aatacggcga
       61 cgataccgcc cgggttttcc cgtttgatgg cttgcaagta ccactcgatg gggtgggcga
      121 tctcaaagcc gtcaccaagt gcgctccgca actgcatctc acacccggga ttggccgagg
      181 ccaccttttt caccccggta gaacgcacct gggatgcctt tcggtttccg agttcttgag
      241 aagcctccgg ttggaggacg gtgtagagac ctgcagcgcc acagcacatg ccggtggggt
      301 cgatctcgat cacctcgtag ccggctgatt cgaggatcag cctcggctct gcggtgacct
      361 tctgggcgtg acggagatgg caggggtcct ggattccgat tggccccaat gagacggctg
      421 cccgtggaag caagccctgc tcgatggccc gggccactgc caccgtgacg tccatcgcgg
      481 tcgatccgac gccctctcca ccgtccgccc agtggtcgta atccttgaga tgagccgagc
      541 acccggccgc agttgccacc accatgtcgt acccgctcag gatcggggtg ttgcgctcgg
      601 ctagccggtg ggcctcaggt gccttgccgt cgtgggcagc gagggcgcca caacaggtct
      661 gtccgcccgg aacttcgacc cgatatccgg ccatctccaa caattcgacc gcggcgtggt
      721 tcaccggttg gaaccactgg tcctggatac aaccggccag caaagcaacc ctccctgacc
      781 tcccctcgcc cccgttgtgg ccgacaaccg atcccggtcg actctgcagc tcccgcatcc
      841 ccgccgccag ccgacgccag cgcctggggg ccatcttggt ggcccggacc cgttgcccga
      901 ggctgagggc tccggtcccg aacctgagcg atcgtcgaga cccgaggacc gagccgagca
      961 ggaagcctcg gagacgatgg gacatcgaag gccgttgagc gacgatctcg gctcgggttc
     1021 cttccagagt tcggccgtag ggcaccaagc ctggacagac cggctcacag gcgcgacagc
     1081 ccaggcaaaa atcgatgatc cgctcaaaag cggcgtcgac ctcgaccact ccttcgtgga
     1141 ccgcgctcat cgcgtggaga cgaccccggg gggaggccgt ctcgcggccg gtgagccgga
     1201 aggtggggca atgggggagg cacaacccgc actggacgca ggcgttcaac tcgtcggtgg
     1261 tcggatgctc agccgtgacc caacccatca aatgccgccg ggaagccggc ccgggttgat
     1321 cacccgggat gggtcgaact ggccgatgag ccggcgttgg atatcgagac cctgaggcgg
     1381 ggctccccaa gggtcgaagt cagggagaga gccagccggc gaatcgacca ccaccaaccc
     1441 ccctgcattc gcctccgccc actctcggag ggcagttgcg cccgcggcat catccgagcc
     1501 gagacggatc tcacctgcac cgtgaatcgc cagataacgc catccttcgg gcaaacggga
     1561 ggtcgcggcc gcagtcaccg ccggtgggat ccgcatcgac cacttgaatt caccctcagg
     1621 atcgtttggc cagacgtgcc cgggtgtcag ctcaccacct atctggcgag cattggcctc
     1681 cacctcctgg tcgatgcccc aaaggaagac cttcgtcacc ccatcgctct cgagcacggc
     1741 aaggggacgc gaaagcagat gaaggcgatc cggatccgga ttggtgaccg tcgccttggc
     1801 tggcggcatc ggccacaact tgagacagac cgaaaccact accccgaggg cgccgaaggc
     1861 accaaccacg aaccgggaaa ggtcgtagcc ggagacgttc ttcacgaccc gcccaccggc
     1921 acgcacgatc cgcccgtccc cggtaaccag agtcacctcg agcaggcgct cccgggtgcc
     1981 aagaaggcgt gcccgcttca gcgaggaaac tccggccgaa atcactccgc cgatggtcgc
     2041 cggccccgga ttctcgggca tgacggccgt ttggctcctc ccggccagca tctcttcgaa
     2101 tttggccacc ggcgtccccg acccgagcac cagagtcaga tcgtccggtt cccaggcctc
     2161 aaccgcatcg aggcggtcgg tcacaaggac caggtcgggc ttctcaggcc ggccatagcc
     2221 ttgatgagag ccaccacccc agacctggac cttgagccgg ttctcggtgg cgaaacggag
     2281 gaccttggag acttcttctg gggaggcagg agcaagaccg gtcccggcca tcgagcctgg
     2341 agggatctcg gcttccatgc tccggagggt ggccgggatg gcgccgggga ccactgtcaa
     2401 acccaggctc cctcgggtat ctccttgcgg accccaccga agtcaaagca gagcgacccc
     2461 tggggcaaga cctttgaagg gttgaaggtt gcggcggggt caaacacctt cttgagcctg
     2521 gcctgggcgt cgagatcgag ctcgctgaaa ttcaaccgca tcaaatctcg cttctcgatg
     2581 ccgatcccgt gctctccgga caaggcgccc ccatttcgga gacagagggc gacgatctcg
     2641 tctccggcct tacgcacctt ctccgcagca atcgggtccg aggagtcaaa ggcgatcagg
     2701 ggatggaggt tgccgtcacc agcgtgaaag acgttgacca ggaggaggcc ataacgatcc
     2761 gcgatcgcgt cgacctcgag catcgtctcc accagtcggg ttcgggggac caccgtgtcg
     2821 tggaggtagt agtcaggcgc caactgggcg accgccccga aggccgactt ccgacccttc
     2881 cacaggagtt ggcgctcggc ctcggtcgcc gccacccgga tcgcctgtga attccggcgg
     2941 gtagcaatgt cttgaacaag cgccgactcg gcttcgacgc cggcggtctc accaaccaac
     3001 tcgacgagga ggatcgccgc tcggttggtg gggaggccgg cctgcagcca gttctcgacg
     3061 gcctgcatca tccgctgatc catcatctcc agggcggcgg gcacgcagcc tgcggcgatg
     3121 atgtccgaga ccgtaccgca ggcgtctccg actcgatcga agtcgatcag caaggtccgc
     3181 acgtcaggat cgaccggaat cagccgaacc agagccctgg tgacaacacc cagggtgccc
     3241 tcggagccca cgatcagccc cttgagatcg agaccagggg tgtcgggggc aagaccgccg
     3301 agaaccatga cctcgccgcc ggcgaggacc acctcgagac cgagaatgtg actggtagtg
     3361 ctcccctcgg ccaagcaatg cgggccaccg ctgttgttgg ccacgttgcc gccgaccgtg
     3421 cacgccgact gcgatgaagg gtcgggtgcg aattggaggc caaagggcgc cgcggcacgg
     3481 gtcagatcca gattgatcac cccagcgcct acccacgccg cacggttgtc gacatcgatg
     3541 tccacggcgt tgagcgaggt tgtcgacacg acgaccccac cttcggtggg actggctccc
     3601 gcggtgagcc cggtcccggc gccgcgggcc accaccggag ctccgttggc ctcggcgatc
     3661 ttgacgattt cggccacttc ctcggtggtt cgagggaacg ccacgcaatt gggatggccg
     3721 cggaccaggg aagaggcgtc cttggagtac agatagagct ccacgggctc gtggaggacc
     3781 cgatcggcgc cgagaagttg ttcgagatcc gagatcagcg acattgcgcc ggaggctact
     3841 gggacgactt gggcacccca ctcgctgcgc tactccgctt cggtctctgg cctcccacta
     3901 tgaccaagct ctctgacgaa attggagaag atcccagcgg gggaggccaa ctccaggagg
     3961 aacccgagtt ggagaagccc aagactggcc agcgagaggt tgaagagctg aatagcggcg
     4021 taaatcccgc tgaagaccat ggcgatcgtc cagctgactc tgggtaccca gatagtggga
     4081 tcgggatgtc ggttgatcca gacatcgatc aggatcgaaa tcactaaggc ggctgaaccc
     4141 acacggatgg tcgactcgag atcctcggtc atgtagaaga ccgggatcag gagcaaggcc
     4201 agaaatgcga cagtgaaagc ctgacggacg atggtcttca cccgccacag g
//
LOCUS       DBNHXU010000300         9392 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2090153, whole genome shotgun sequence.
ACCESSION   DBNHXU010000300 DBNHXU010000000
VERSION     DBNHXU010000300.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 9392)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 9392)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9392
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2090153"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1223
                     /locus_tag="AC161X_07510"
     CDS             <1..1223
                     /locus_tag="AC161X_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HIR4989435.1"
                     /translation="SNSVKDEARANGDDVFSETQRAAAVVLDAKNFEVLALASYPDFD
                     PNLFVSGIDANTFQGLNDSNAFNNLAISGLYPPSSTFKAITYTVKMEENLPLDPDLEG
                     VDVSNQTVHCDGKLVLSKLEDGSQPPKFDWYYPRDLDWLDIHGAFEHSCNIYFWSVAM
                     GTWQKYKGTPQENIIQDWAKSLGYGAPTGIDLTAEADGTVPTRELFEEWAAYQLENPD
                     QPPLLEPNRLELASPWLGGDLVDMAIGQGAFTSTPLQVAVSYAALVNGGRIMEPRVVN
                     RVVDADGNTVFTPEARLVRTVDIDPDTRASLLTDLNRVVTVGTAATAFSDFGPGLENV
                     GGKTGTGQTSKNRDNHAWFVGVMPVDDPKYIVVVLIDEGGSGGRIAAPVARRIMQYLM
                     GNDLTDIVEGEQAD"
     gene            1223..2377
                     /gene="rodA"
                     /locus_tag="AC161X_07515"
     CDS             1223..2377
                     /gene="rodA"
                     /locus_tag="AC161X_07515"
                     /inference="COORDINATES: protein motif:HMM:TIGR02210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein RodA"
                     /protein_id="HIR4989436.1"
                     /translation="MTVLNRLRDIAVDRRNNRSDQVLFGTMLALSGFGLLMIYSATRS
                     AGSVSMERQMIFVAAGLTIYLISSNIDYREYKGVIPWISVGLLIALTAVYLFEPVNGA
                     RRWIPLGFFKLQPAEFAKVAVIILLAVILSPHSRDEIGRRSLRWTHIFQALAVVAVPA
                     VLIYFEPDLGTTLVFGFILFAMLFTAGATWRQLVALASAAVLGGLVIYQQGWLAEYQL
                     NRILVLFKPTEVDPLGIGYNLAQSKMAVGSGQLIGKGLFQGTQTNFEYVPEQETDFIF
                     TAVGEQLGFIGAILVIAAFSVIVWRLLVIAANARDRFGALIAAGIAAMVVFHVFVNVG
                     MTIGIMPVTGIPLPFLSSGGSFYMAMTLALGIANSIWLLRTPVPGENQLS"
     gene            2397..2987
                     /locus_tag="AC161X_07520"
     CDS             2397..2987
                     /locus_tag="AC161X_07520"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002627415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HIR4989437.1"
                     /translation="MYRRSHDGWVEVICGPMFSGKSEELIRRVTRSKIARIPVQTFKP
                     RLDNRYADSEVVSHSSLKVDAIPVETSDQLLRAVEDVTEVIGIDEGQFFDQGLVEVVE
                     QLAAAGKQVIIAGLDTDFLRRPFEPIPTLCHRAEYVTKMLAVCHKCGGPGMYTQRIVH
                     SEDLVVLGAQGAYEARCRVCYDPTEPEQPELGLVVG"
     gene            3242..4792
                     /locus_tag="AC161X_07525"
     CDS             3242..4792
                     /locus_tag="AC161X_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rne/Rng family ribonuclease"
                     /protein_id="HIR4989438.1"
                     /translation="MALFRRKSPQIVRKSSVEGTVEERKTLRVKGERVEVTRVEARPA
                     PSKARKDKKRQPSKSSHGRNGLSTTRTRSSRPMDDEVIVPVETARKQMLVRVARGQTQ
                     MVILEGPVLVEHYVARQESNSVAGNIYLAKVRNVLPGMEAAFLDFGAPKNGVLYASDV
                     ASTGNGGGRSNKRIENVLKEGDEVLVQVTKDAMGAKGARLTGIPSLPGRYLVLVPESD
                     SVGISRRLPDEDRSRLREVINKVRPVGFGVIVRTAALHASAEELAADISRLLKIWSQI
                     QEEAGDGRAPRMVHEEPELLIKVIREHFTADFRKLMIDDHAAYETVVGYLRSTAPDLV
                     SKVQLYEDTVPLFERYHVDDQLKKALDRRVYLPSGGHLVMDRTEALTVIDVNTGKFVG
                     SSTLEDTVLQNNLEAAEEIGRQLRLRDIGGIIVIDFIDMESIENQQKVLRRLKETLAK
                     DKTRTQVFEVSHLGLVEMTRKNVSAGLLEHFSSKCEACAGRGIIIHEHVIASEDIDVE
                     VSAVADYE"
     gene            4910..5209
                     /gene="rplU"
                     /locus_tag="AC161X_07530"
                     /pseudo
     CDS             4910..5209
                     /gene="rplU"
                     /locus_tag="AC161X_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011292605.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
     gene            5502..5753
                     /gene="rpmA"
                     /locus_tag="AC161X_07535"
     CDS             5502..5753
                     /gene="rpmA"
                     /locus_tag="AC161X_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HIR4989439.1"
                     /translation="MSKTKAGGSSKNGRESHAKRLGPKVFDGQAVSAGAILVRQRGTG
                     IHPGDNVGRGSDDTLFALSAGTVKYGTRANRRLVNVLPE"
     gene            5754..7004
                     /gene="obgE"
                     /locus_tag="AC161X_07540"
     CDS             5754..7004
                     /gene="obgE"
                     /locus_tag="AC161X_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012032004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HIR4989440.1"
                     /translation="MFVDRVRVALRAGDGGAGVAAFVRRKGQPKGKPNGGNGGKGGDV
                     ILRVDTSLATLLRYQRNPHHSAEKGTHGEGDMRHGKSGEDLVLSVPAGTVVFDEDGVM
                     IADLVEPGQQVIALSGGRGGRGNAAFVTPAKRAPAIAEQGEFGREEWFTLEVKLLADA
                     ALIGFPNAGKSTFISKVSAAKPKIADYPFTTLEPNLGVVSMGDREFVLADIPGLIEGA
                     AEGKGLGHEFLRHIERARVLVVLLDPSPIQEHSPERQLEILSREIASYSGDLAARPRV
                     NLLSKSDLGDVSELAGRLEAIPVSAMTGDGLSAALHAIGDLIDEAERAAPERKGFVLH
                     RPLGPTFEILREGGAWRVHGTAAQRAVGFADLTMPEAADMAARRLARLGVDDALIAAG
                     ALPGDEVRIGDLSFEFVPDIGEEE"
     gene            7014..7877
                     /locus_tag="AC161X_07545"
     CDS             7014..7877
                     /locus_tag="AC161X_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989441.1"
                     /translation="MDFDSPQAVSEALVSVDYLPNDRIAQVVFLAARLDKPILVEGPA
                     GVGKTELAKALSRVTGRELIRLQCYEGLDEAKALYEWNYKKQLLRIQADTDHEWEEIE
                     QDIFSEEFLLTRPLLAAIRSADPVVLLIDEVDRVEVETEALLLEILSDFQVSIPELGT
                     IEASTQPLVVLTSNNTRELSEALKRRCLFLHIDYPDPAREQQILNARVPGLSEHLAAQ
                     VANVARSIRNLDLKKAPSVSETIDWARTLLALSIEEVDGAVLGDTLHVLLKYQSDIEK
                     AAKELASTPGD"
     gene            7880..9310
                     /locus_tag="AC161X_07550"
     CDS             7880..9310
                     /locus_tag="AC161X_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR4989442.1"
                     /translation="MLGVITGFIDELREAGVPVSMVEAIDAMRAVEAINVGDRVALRE
                     TLRATMVKNLRHERAFDTAFEVYFSTIPVPSDVSPLPEQGKDLPRSESEDRPQSQPFD
                     LDALLERLFQALADMDGAALRQGAQTAVDQLAGMEPGRPVGGTYYLYRTLRRLDLAGL
                     EERLLQALIGDSEMSEFDQRLLREEVQSRIEELREEIQEEIRRRLVADRGRDAVARTL
                     RRPLIEDVDLMHATAGDLKEMDAAIGPLTRKLAARLARRRRKRTGGLDFRRTVRRSLA
                     TGGVPADPQFRHPRPHRPEVWLLCDVSGSMATFSRFTLQFTHAMATHFSKLRSFAFVD
                     TVDEVTDFFGPGVDFGSALERVATEAEVVWLDGHSDYGNSLENFVKRYGPQLTPRTTV
                     IITGDARNNYRPPRDQVLAEVTRMARAVVWLNPEPSTYWDTGDSMMSHYARHCSSVHE
                     VRTLRQLEQFVEELTLPTGRSLQNVL"
BASE COUNT         1815 a         2915 c         2900 g         1762 t
ORIGIN      
        1 tctccaactc ggtcaaggat gaagcacggg cgaacggcga cgacgtgttc agtgagaccc
       61 agcgagcggc tgcggtcgtg ctcgacgcca agaacttcga agtcctggct ctcgcctcct
      121 acccggactt cgacccgaat ctgttcgtca gcggaatcga tgccaacacg tttcaggggt
      181 tgaacgacag caacgccttc aacaacctgg cgatcagcgg cctctatccg ccctcgtcca
      241 cattcaaggc gatcacctat acggtgaaaa tggaggagaa ccttcctctc gaccctgatc
      301 tggagggagt cgatgtctcc aatcagaccg tgcattgtga cggcaagctg gtcctctcca
      361 agttggaaga tggttcccag ccaccaaagt tcgactggta ctacccgcga gacctcgatt
      421 ggctcgacat ccatggcgcc ttcgagcatt cctgcaacat ctacttctgg tcggtggcga
      481 tgggcacctg gcagaagtac aaaggcacgc cccaagagaa cataatccag gattgggcca
      541 agtccctcgg ctacggagcg ccaaccggaa tcgacctgac cgccgaggcc gacggcaccg
      601 ttccgacccg tgagttgttc gaggaatggg ctgcctacca actggagaat ccggaccaac
      661 ctccgctgct ggagccgaac cgactcgagt tggccagccc ctggctcggt ggtgacctcg
      721 tcgacatggc gatcggccag ggcgcattca cctcgacacc gctccaggtg gcggtctctt
      781 atgccgccct ggtcaacgga gggcggatca tggagccccg cgtcgtcaac agagtcgtcg
      841 acgccgatgg caacaccgtg ttcacacccg aggcccggct tgtgcgaact gtcgacatcg
      901 accctgacac ccgggcctcc ttgctcaccg acctcaatcg ggtggtcact gtcggcaccg
      961 ctgccactgc tttctcggat ttcgggccgg ggctggagaa cgtcggtggc aagaccggga
     1021 ccgggcagac ctcgaagaac cgcgacaacc acgcctggtt cgtgggtgtg atgcccgtcg
     1081 acgacccgaa gtacatcgtt gtggtcctga ttgatgaggg cgggtctggt ggccgtatcg
     1141 cagctcccgt cgcccgtcgc atcatgcagt acctgatggg caacgatctg accgacatcg
     1201 tcgaaggcga gcaggccgac tgatgacagt gctcaaccga ctcagggaca tcgccgtcga
     1261 tcgtcgcaac aaccggtcgg accaggtctt gttcggaacc atgctggcct tgtccgggtt
     1321 cggcctgctc atgatctact cggccacccg aagcgcggga tcggtctcaa tggagcggca
     1381 gatgatcttc gtcgctgccg ggctcaccat ctatctgatc tcgtccaaca tcgactatcg
     1441 ggagtacaag ggagtgattc cctggatctc ggtgggtctg ctgatcgcat tgaccgcggt
     1501 ctacctcttc gaaccggtca atggtgctcg gcgttggata ccgctcggtt tcttcaagct
     1561 gcaaccggcg gaattcgcca aggtggccgt gatcatcctg ctggcggtca tcctctcccc
     1621 gcacagccgc gacgagatcg ggcgtcgcag cttgcgttgg acccacatct tccaggcact
     1681 ggcggtggta gctgttcccg ccgtcctgat ctacttcgag ccggatttgg gcaccaccct
     1741 ggtctttggg ttcatcctct tcgcgatgct gttcaccgcc ggcgccacct ggcgacagct
     1801 ggtggcattg gcatcggcag cagtgcttgg agggttggtg atctaccaac agggttggct
     1861 ggccgaatat cagctcaacc ggatcctggt tctgttcaaa cccacggagg tcgatcccct
     1921 ggggattggc tacaaccttg cgcaatcgaa gatggcggtt ggctcaggac agctgattgg
     1981 caagggactc ttccagggga ctcagaccaa cttcgagtac gttcccgagc aggagaccga
     2041 cttcatcttc accgcggtgg gggagcagct cggattcatc ggcgccatcc tggtgattgc
     2101 cgccttctcg gtgatcgttt ggcgtctgct tgtgatcgcg gccaacgcac gcgacaggtt
     2161 cggtgctctg atcgctgcag ggatcgcggc catggtggtg ttccacgttt tcgtgaatgt
     2221 ggggatgacg atcgggatca tgccggtgac ggggatcccg cttcccttcc tgtcctctgg
     2281 cggctcgttc tacatggcga tgaccctggc cctggggatc gccaactcga tctggctgtt
     2341 gcgaaccccg gttccgggcg agaaccagct gagctgagcg tctactctcg ccctggatgt
     2401 accgcaggag tcacgacgga tgggtcgagg tcatctgcgg gccgatgttc tccgggaaaa
     2461 gcgaagagct gattcggcgc gtcacccgat ccaagatcgc ccgaatcccg gtgcaaactt
     2521 tcaagcctcg actcgataat cgctacgccg actccgaggt ggtctcgcat tccagtctca
     2581 aggtggacgc gatccccgtc gagacctctg atcagctgct gcgagccgtc gaagatgtca
     2641 ctgaggtcat cgggatcgat gaaggccaat tcttcgatca gggcttggtc gaggtcgtcg
     2701 aacaactcgc cgcagcgggc aaacaggtga tcattgccgg tctcgacacc gacttcctgc
     2761 gtcgcccttt cgagccgatc cccacccttt gccaccgggc cgaatatgtc accaagatgc
     2821 ttgccgtctg ccacaaatgt gggggacctg gcatgtacac ccagcgcatc gtccactcgg
     2881 aggatctggt cgtccttggc gcccaagggg cgtatgaggc ccggtgtcgg gtgtgttacg
     2941 acccgaccga acctgaacag ccagagctcg ggctggtagt gggataggtc atcggcacct
     3001 ttcctggtat ccttctttgg tccgccagac gtgacagcct tcccctttct cggagtgtct
     3061 cgttgatgaa cacccttgcg tacctgcagt caggtgcgac ccaaatcgat ttccaagaca
     3121 accgccccca acccgggtgg atgtctcgcc gccgtctggc agatgttgtc ggcaacggat
     3181 tccccgccag gaatcgggct gagtctgttg ccctgtccaa attccagagg agtgaaaccc
     3241 tatggctcta ttccgtcgaa agagcccgca gattgtccgc aagtcctccg tcgagggcac
     3301 cgtcgaggag cgaaagaccc ttcgggtaaa aggcgagcgg gtcgaggtga cccgggtcga
     3361 ggcacgaccg gcgccatcca aggcgcgtaa agacaagaag cggcagccgt cgaagtcttc
     3421 tcatggccgc aacggcctga gcaccacccg aacccgttcc agtcgcccga tggacgacga
     3481 ggtgatcgtc cccgtcgaga ccgcacgcaa gcagatgctg gtccgggttg cccgaggcca
     3541 gactcagatg gtcatcctcg aaggaccggt tctcgtcgag cactacgtgg cgcgccagga
     3601 gtcgaattca gtcgccggca acatctatct ggccaaggtt cgcaacgtcc tccctggcat
     3661 ggaggcggca ttcctcgact ttggtgctcc caagaacggc gtcctctatg catccgacgt
     3721 cgcctcgacc ggcaatgggg gcgggcgtag caataagcgg atcgagaacg ttctcaagga
     3781 aggcgacgag gtcctggtgc aggtgaccaa ggacgccatg ggggccaagg gcgcccgtct
     3841 caccggaatc ccctcacttc ccgggcgtta tctggttctc gtgcccgagt ccgacagcgt
     3901 cgggatcagc cgtcgtctgc cggacgagga caggtcccgt ctccgggagg tcatcaacaa
     3961 ggtgcgccca gttggattcg gcgtgatcgt gcgcaccgct gcgctgcatg ccagtgccga
     4021 agaattggcc gccgacatct cacgtcttct caagatctgg agccagatcc aggaggaggc
     4081 aggagacggc cgtgctccaa ggatggtcca cgaggagccg gagctcctga tcaaggtcat
     4141 tcgcgagcac ttcactgcgg actttcgcaa attgatgatc gacgaccacg ccgcctacga
     4201 gactgtggtc ggctatctga ggtcaactgc gcccgacctg gtgtccaagg tccagctcta
     4261 cgaggacacg gtgcccttgt tcgagaggta tcacgtcgac gaccagttga agaaggccct
     4321 cgaccgccgg gtgtatctgc cctcgggcgg gcaccttgtg atggatcgca ccgaggctct
     4381 caccgtcatc gacgtcaaca ccggcaagtt cgtgggctca tcgaccctcg aggacacagt
     4441 tcttcagaac aacctggagg cggcggagga aatcggacgt cagcttcggc tgcgtgacat
     4501 cggcgggatc atcgtcatcg acttcatcga catggagtcg atcgagaacc agcagaaggt
     4561 cctacgccgt ttgaaggaga ctctggccaa agacaagacc aggacccagg tgttcgaggt
     4621 ttcccatctc gggctcgtcg agatgacccg aaagaatgtc tcagctggcc tgctcgagca
     4681 cttctcgtcg aagtgtgagg catgtgccgg ccggggaatc atcatccacg agcatgtgat
     4741 cgcgtccgag gacatcgacg tcgaggtctc ggcggtcgct gactatgagt agtcggcgga
     4801 cattggtatt cgacacttgg tgccgggacc aatcccgctg ccaagtacct agtacccgaa
     4861 gccactgtta tcctgaaccc gtaaacccct ttttggaagg tctttaacca tgtacgccat
     4921 cattcgcgcc ggaggcaaac aggccaaggt gcaatcaggt gacgtgattg acatcgaacg
     4981 ggtcaaaggc gactcggagg aactgagttt ttccccgttg cttgtggtcg atgacaaggg
     5041 caccgcggtc tccgaccggt cgctcttggc caacgccacc gtgaccgcga aggttctcgg
     5101 cgagagccag ggcccgaaga tcgacatcta caaatacaag aacaagaccg gctacagccg
     5161 tcggcagggc catcgtcaga agtacacccg gatcgaaatc accgggatcg agctccccgg
     5221 agctgccaag ccgaaggcaa aggcagccac tcccaaaact gctgccgcac cagccacgga
     5281 tatgcccgag gccccttcgg tggtcgaggg cgcggaggca cccgcaaagt ccgccgctcc
     5341 taagcctgca gccaaagctg ccaagcctgc tgctgccaaa gccgccaccc cgaaggccgc
     5401 cgccaagccg gccgctgcca aggcggggac ctccaagccg gctgcgaaga agccagccac
     5461 gcctaagacc ccaaagcccc cagcttccga ggagtaatca aatgtcaaag accaaagcag
     5521 gcggctcgtc caagaacggt cgcgagtccc acgccaagcg tctggggccg aaggttttcg
     5581 acggccaagc tgtcagtgca ggcgccatcc tcgtccgcca gcgcggaacg ggcatccacc
     5641 cgggcgacaa tgtcggccgg gggagcgatg acacgttgtt cgcgctgagt gccggcaccg
     5701 tcaagtacgg gacccgggcc aaccggcgtc tcgtaaacgt cctccccgaa taggtgttcg
     5761 tcgaccgggt ccgggtcgcc ctgcgggccg gtgacggagg agccggagtc gctgccttcg
     5821 ttaggcggaa aggccagccc aagggcaagc caaacggggg caatgggggc aaaggcgggg
     5881 atgtcatcct cagggttgat acttcgctgg ccaccctcct ccgataccag cgcaatccgc
     5941 atcactctgc cgagaagggg acccatggcg agggggacat gcgccacggc aaatcaggag
     6001 aggatctggt cctatcagtc cccgccggca cggtggtttt cgacgaagac ggtgtcatga
     6061 tcgccgacct cgtcgaaccc ggtcagcagg tcatagccct cagcggaggt cgtggaggcc
     6121 gtggcaacgc tgctttcgtc acccctgcca aacgagcccc cgccatcgcc gagcagggtg
     6181 aatttggtcg cgaggagtgg ttcacgctcg aggtgaagct ccttgccgac gcggccctga
     6241 tcggattccc caacgcaggc aagtccacct tcatctcaaa ggtctcggcg gccaagccca
     6301 agatcgcgga ctacccattc acaacgctcg agcccaacct cggcgtcgtc agcatgggcg
     6361 atcgcgagtt cgttctggca gatatccccg gcctgatcga aggggctgcc gaaggaaagg
     6421 gccttggtca cgagtttctg cgtcacatcg agcgagcccg ggtcctggtg gtgctcctcg
     6481 atccgtcgcc aattcaggag cactccccgg agcgccaact cgagatcctg agcagggaga
     6541 tcgccagcta ttcgggtgac ctggcagcta ggccccgggt caatctgctc agcaagtccg
     6601 atctgggtga tgtctctgaa ctggctggcc ggttagaggc gatcccggtg tcggcgatga
     6661 ccggggacgg gctcagcgca gctcttcacg caatcggtga cctcattgac gaggcggagc
     6721 gagccgcccc ggagcgcaag gggttcgtgc ttcacagacc actggggccc actttcgaga
     6781 ttcttcgaga aggtggcgcc tggcgggtcc atggaacagc agcccagagg gctgtcggat
     6841 ttgccgacct caccatgccc gaagccgccg acatggccgc ccgccgtctc gcccgccttg
     6901 gtgtggacga tgcattgatc gccgcggggg cgctccctgg tgatgaggtt cgaatcgggg
     6961 atttgagctt cgagttcgtc cccgatatcg gtgaggagga ataatggtgc ccgatggatt
     7021 tcgatagccc ccaggcggtc tccgaagctc tggtctcggt cgactatctc cccaacgacc
     7081 ggatcgccca ggtcgtattc ctggctgccc gactcgacaa gccgattctg gtcgaagggc
     7141 cggcaggcgt cgggaagacc gagttggcca aggcgctgtc cagggtgacc ggccgagagt
     7201 tgatccggct ccagtgttac gagggccttg acgaggcgaa agccctgtac gagtggaact
     7261 acaagaagca gctgctccgg atccaggccg acaccgatca cgagtgggag gagatcgaac
     7321 aggacatctt ctccgaggag ttcctgctca cccgaccctt gctggccgcc atccgctccg
     7381 ccgatccggt cgtcctcctc atcgacgaag tcgacagggt cgaggtcgaa accgaagctc
     7441 tcctcctcga gatcttgtcc gacttccagg tgtcgattcc ggagttggga accatcgaag
     7501 catccacaca gccgctggtg gttctcacct cgaataacac cagggagctc agtgaggccc
     7561 tgaaacggcg ttgcctcttc cttcacatcg actacccgga tcccgcccgg gagcagcaga
     7621 ttctcaacgc ccgtgtcccc ggcctctccg agcaccttgc cgctcaggtc gccaatgtcg
     7681 ctcgctcgat ccggaacctg gacctgaaga aggcgccttc ggtctcagag acgatcgact
     7741 gggcgcggac tttgcttgcg ttgtcgatcg aggaagtcga cggggcggtg ctcggggaca
     7801 ccctccatgt gctcctcaag taccagtcgg atatcgagaa ggcggccaag gagctggcct
     7861 cgactcctgg cgactgagaa tgctcggcgt catcaccggc ttcatcgatg agctgagaga
     7921 ggccggagtt ccggtgtcga tggtcgaggc catcgacgcg atgcgagctg tcgaagcaat
     7981 caatgtcggc gaccgggtcg cactgcgaga gacactccga gcgacgatgg tgaagaacct
     8041 cagacacgag cgagcattcg acaccgcgtt cgaggtgtat ttctcgacga tccccgttcc
     8101 atccgacgta tcgccgctgc ctgagcaggg gaaggacctt ccccggagtg aatcagagga
     8161 tcgtccccag tcccagcctt ttgacctcga cgcgcttctc gagcggttgt tccaggcgct
     8221 cgccgacatg gacggggcgg ccttgcgcca gggtgcgcaa accgccgtgg atcaactggc
     8281 gggaatggag ccgggacggc cggtgggcgg gacctattac ctatatcgga ctttgcgccg
     8341 cctcgacctt gcaggacttg aggagcggct tctgcaggcg ttgatcggtg acagtgagat
     8401 gagcgaattc gatcagcgtc ttctccgcga agaggtccag tcgcgcatcg aggaattgcg
     8461 agaggagatc caggaggaga tccgaaggag gctggttgcc gaccggggac gggacgctgt
     8521 ggcccgcacg ctgcgacgac cgctcatcga ggatgtggac ctgatgcatg ccacagccgg
     8581 tgacctcaag gagatggatg ccgcgatcgg gccgctgact cgcaagctgg ccgcccgact
     8641 ggcccgacgc cgccgcaagc gcactggcgg gctcgacttt cgtcgcactg tccggcgctc
     8701 cctggcaacg ggaggggttc cggccgatcc tcaattccgg catccccgtc cgcaccgtcc
     8761 tgaggtctgg ctgctttgtg atgtgagtgg gtcgatggcg acgttctccc gattcaccct
     8821 tcagttcacc cacgccatgg ccacccattt ctcgaagctg cgctcgttcg cgtttgtcga
     8881 caccgttgat gaggtcaccg acttcttcgg cccgggtgtc gacttcgggt cggcgttgga
     8941 aagggttgca accgaggccg aggtcgtttg gctcgacggg cactctgatt acgggaactc
     9001 gctcgagaac ttcgtgaaga gatacgggcc tcagttgacg ccgaggacga ccgtgatcat
     9061 caccggggat gcccgcaata actaccggcc gccccgtgat caggtcctgg ccgaggtgac
     9121 gaggatggcg agggccgtgg tatggctcaa ccccgagccc agcacctact gggacaccgg
     9181 agactcgatg atgtcccatt acgcccggca ctgcagctcg gtgcatgagg tgcggacctt
     9241 gcgccaattg gagcagtttg tcgaggagct gacgctgccc acgggtcgct cacttcagaa
     9301 tgtcctctaa accggcggcg gctaactgcc gatgaaaggg gatacgccct ctgtggggca
     9361 attaccagag gtaaagaaat gggctcgcgg at
//
LOCUS       DBNHXU010000301         2273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5466003, whole genome shotgun sequence.
ACCESSION   DBNHXU010000301 DBNHXU010000000
VERSION     DBNHXU010000301.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5466003"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2259
                     /locus_tag="AC161X_07555"
     CDS             <1..2259
                     /locus_tag="AC161X_07555"
                     /inference="COORDINATES: protein motif:HMM:NF012311.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HIR4989443.1"
                     /translation="GNAGVEASIFGRDFGEISGIAPDAWVVMYSACGDQGCFGGDLAG
                     AIDQAVADGVDVINYSIGSDTPGLDGIDDIAFLFADFAGVFTSVSAGNAGPGASTVGA
                     PASTPWVITVGASEQNRAFVAQVKIDNGSKSRWSHFFKKDSGIFEGASVGAGTHGKFP
                     FVDSADHGNEACDPAVDFDPDITGMVVLCPRSPGRTEKSLAVFEQGGVGMVMYNADDV
                     QSLLTDNHYVPSVLVNHSDGLALKAYIAKAGSNARVRLTAAEAEHQRGNEMSEFSSRG
                     PVGEPASPDIIKPDVTAPGVQILAGNTPTPTASAPGELFQAISGTSMSSPHVAGLLAL
                     LKQAHPEWTPAMARSALMTTARQDVRQEDGHGRADPFDFGAGHVDFSGRTTSEGSVFN
                     PGLAYDAGVFDYLGFLCDTSASYLTPAFTGLSCDDFGGIGFQTQSENLNYPSIGVSEI
                     PGSKTVIRTVTNVSDKTTSYRAKVDEPRGFDVEVSPNRIKLAPGESVTFEITFTNKNA
                     PVGTWRFGSLTWDSGKNKVRSPIAVKAAAIEFPNSVSGTGVTGSATIPVAFGYTGTYT
                     ATAHGLAADASQSGVVLHDGPDQEFDPVEDVPQGDVVAHPFTLSGSAFLRITMGEDDL
                     TSVDAAATDIDLYLYFNGALIAQSTAGGTDELIDLEAPADGNYVLYVHGWQVVDPPPG
                     VGYTFHLWDVPTAASSLSINAATPTTATIGTIGNVVVDWTGLAPGGSYLGAVSHSDGA
                     TDFGLTLVAVDS"
BASE COUNT          422 a          715 c          715 g          421 t
ORIGIN      
        1 gggaatgccg gtgtcgaggc ttcgatcttc ggtcgggact ttggcgagat ttccggaatc
       61 gcccccgacg cgtgggtggt catgtactcg gcgtgtggag accagggctg ctttggaggt
      121 gacctcgccg gggctatcga ccaggcggtc gccgacggtg tcgatgtgat caactactcc
      181 atcggaagcg acaccccggg gctggacggg atcgatgaca tcgcattcct gtttgctgac
      241 ttcgcgggcg tcttcacttc ggtgtctgca ggcaacgccg ggccgggggc aagcacggtt
      301 ggcgctcccg ccagcactcc atgggtcatc accgtgggcg ctagcgagca aaaccgagcc
      361 ttcgtggccc aggtcaagat cgacaatggg agcaagagcc gctggtctca tttcttcaag
      421 aaggactccg ggatcttcga aggagcctcg gttggtgcag gcacccatgg aaagttcccg
      481 ttcgtcgatt cggcggatca cggcaacgag gcttgtgatc ctgccgtgga cttcgacccc
      541 gacatcaccg gaatggtggt cctctgcccg aggagcccgg gtcgaaccga gaagagcctt
      601 gctgtgttcg agcagggcgg ggtcggcatg gtgatgtaca acgctgacga cgttcagtcg
      661 ctgctcaccg acaaccatta cgttccatcc gtcctggtca accacagtga cgggctggct
      721 ctcaaggcat acattgccaa ggcgggctcc aacgcccggg tgcgcctcac ggcagcggag
      781 gcggaacacc agcggggcaa cgaaatgtcg gagttctcct cacggggccc ggttggcgag
      841 ccggcctcgc cggacatcat caagcccgat gtaaccgcgc cgggggtgca gatcctcgcc
      901 gggaacacac ccactccgac tgcgagcgcc cctggtgagt tgttccaggc gatctctggc
      961 acctcgatgt ccagtccgca cgtcgcaggg ctactggcac tgctcaagca ggctcatccc
     1021 gagtggacgc cggcgatggc caggtcggcc ctgatgacga cagcccgtca ggatgtccgg
     1081 caagaagatg ggcacggccg agccgacccc tttgacttcg gcgccgggca tgtcgacttc
     1141 agcggaagga cgacttccga aggatcggtg ttcaatcccg gcttggctta cgacgcagga
     1201 gttttcgatt acctcgggtt cctctgcgac acgtctgcca gctatctgac gcccgcgttt
     1261 accggcctca gttgtgacga ttttgggggt atcggcttcc aaacccagtc ggaaaatctc
     1321 aactacccct cgatcggggt cagtgagata ccagggtcga agaccgtgat ccgaaccgtg
     1381 accaatgtgt cggacaagac caccagctac cgagccaaag tcgacgagcc gcggggcttc
     1441 gatgtcgagg tgagcccgaa ccggatcaag cttgccccag gtgagtcggt gaccttcgag
     1501 atcacattta ccaacaagaa cgcccccgta ggcacctggc gtttcgggtc gctgacctgg
     1561 gactccggga aaaacaaggt gcgtagcccg atcgcggtca aagctgctgc aatcgaattc
     1621 cccaattcgg tgagcgggac cggagtcacc ggaagcgcca ccatcccggt ggcgttcggg
     1681 tacaccggca cctacacggc gacagcccac ggtctcgctg cggatgcgtc acagtcgggc
     1741 gtcgtgctgc acgatggtcc cgatcaggag ttcgatcccg tcgaagacgt gccccagggc
     1801 gacgtggtgg cccatccgtt cacactgtcc ggctcggcct tccttcgcat aacgatggga
     1861 gaggacgacc tcaccagtgt cgacgctgcg gctacggaca tcgatctgta tctgtacttc
     1921 aacggcgccc tgatcgccca gtccacagcc ggtgggaccg acgagctgat cgatctcgag
     1981 gcaccagcag atgggaacta cgtcctgtac gtccacgggt ggcaggtcgt cgatccgcct
     2041 ccgggggttg ggtacacctt ccatctctgg gatgtcccaa cggccgcctc gagcctgtcg
     2101 attaatgccg ccaccccgac cactgccact atcggcacga tcggcaacgt ggtggtggac
     2161 tggacagggc tcgccccggg aggctcctac ctcggagctg tgtcccattc cgatggggcg
     2221 accgacttcg gcctcacgct ggtcgcagtc gactcgtagg gccgcccaca att
//
LOCUS       DBNHXU010000302         1765 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2091029, whole genome shotgun sequence.
ACCESSION   DBNHXU010000302 DBNHXU010000000
VERSION     DBNHXU010000302.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1765)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1765)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1765
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2091029"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..400
                     /gene="rpoZ"
                     /locus_tag="AC161X_07560"
     CDS             179..400
                     /gene="rpoZ"
                     /locus_tag="AC161X_07560"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HIR4989444.1"
                     /translation="MKLEQVIDQIGNRFATVVVSARRARQINSYFHQLGVGYGEFVPP
                     QVHSLSRKPLTIAMEEIAEGKVSVDTVEE"
     gene            411..1589
                     /gene="coaBC"
                     /locus_tag="AC161X_07565"
     CDS             411..1589
                     /gene="coaBC"
                     /locus_tag="AC161X_07565"
                     /EC_number="4.1.1.36"
                     /EC_number="6.3.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020379080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004632 - phosphopantothenate--cysteine
                     ligase activity [Evidence IEA];
                     GO_function: GO:0004633 - phosphopantothenoylcysteine
                     decarboxylase activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0015941 - pantothenate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional phosphopantothenoylcysteine
                     decarboxylase/phosphopantothenate--cysteine ligase CoaBC"
                     /protein_id="HIR4989445.1"
                     /translation="MLHGRRVVLCVTGGVAAYKSAYLARRLVEAGADVRVVMSESAGE
                     FIGPKTFAAITGFPPVVSLFDGDLVSPHTELGRWADLVIVAPATAATLGRLAAGISSD
                     AIGATVLATTSPVLLAPAMHTEMWEHPATRRNLEILRDDGYHMVGPETGALAGGDVGV
                     GRVSEPDEIVEVANDILGGEGRGRRVLVTAGGTREPVDPVRFIGNRSTGKMGHAIADE
                     AARRGYDVTLVTTSDLPIDHSVKVVRVDTADEMLEAVGGLHPEIAVMAAAVADFKPAS
                     SKDSKIARSEGLDSIELAATPDILASVVARVDRPFVVGFAAETGGVERAVEKAKRKSV
                     DLLVYNDVSEPGSGFGTDTNRVVIIGRDGSTEDWPLQSKRQVAERLWDRIGEEVRGED
                     "
     gene            1602..>1765
                     /locus_tag="AC161X_07570"
     CDS             1602..>1765
                     /locus_tag="AC161X_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine synthetase N-terminal
                     domain-containing protein"
                     /protein_id="HIR4989446.1"
                     /translation="MRRWYFTSESVTEGHPDKVADAISDSVLDAALETTPGARVACET
                     FVTTRLAVVGG"
BASE COUNT          338 a          527 c          568 g          332 t
ORIGIN      
        1 tctgccgccg acatcgaaga tcgactggaa gtcgcggaga ccgagatcgc agaggcgccg
       61 ggtctgttcg accacatagt tgtcaacgat cacctcgatc gggctgttgc cgagatcgag
      121 gcgctgatta ccggcactgc atcgggtaac ctgtagttac cccctcctcg aaggactcat
      181 gaaactcgaa caagtcatcg atcaaatcgg caaccggttc gccaccgtcg tggtgtcagc
      241 acgccgcgcc cgccaaatca acagctattt ccatcagcta ggtgttggct acggagagtt
      301 cgtgccacca caggtacatt ccctttcccg taagcccctg accatcgcca tggaagagat
      361 cgcagagggc aaagtctccg tcgacactgt cgaggagtag aggagcccag atgctccacg
      421 gacggcgggt cgtcctgtgt gtgaccgggg gagtcgccgc ttacaagtcc gcctaccttg
      481 cccggaggct ggtcgaagct ggtgccgatg tccgggtggt gatgtccgag tcggccggcg
      541 agttcatcgg acctaagacc ttcgcggcaa tcaccggttt cccacctgtc gtctccctgt
      601 ttgacggcga cctggtctcg ccccacaccg agctcgggcg ctgggccgat ctggtgatcg
      661 tcgcccccgc aactgcagcc acactcggtc gtctggccgc cggtatctcg tcggacgcca
      721 tcggagcgac ggtgctcgcg actacctcac ctgtattgct cgcacctgcg atgcacacgg
      781 aaatgtggga gcaccctgcc acccgccgca acctggagat tctccgggat gacggctatc
      841 acatggtcgg gcctgagacc ggcgccctag ccgggggaga tgtcggggtg ggacgggtct
      901 ccgagcccga cgagatcgtc gaagtcgcca acgacatcct cgggggtgag ggtcggggtc
      961 gccgggtcct ggtaaccgct ggtggcaccc gtgagccggt ggatccggtc cgcttcattg
     1021 gcaatcgatc tacaggaaag atgggccacg ccatcgccga cgaggcagcg cgccgcggtt
     1081 atgacgtgac attggtgacg acatctgacc tgccgataga ccactcggtg aaagtggttc
     1141 gagtcgacac cgcagatgag atgctggagg cggtcggtgg gcttcatcct gagatcgcgg
     1201 taatggccgc ggctgttgcc gacttcaagc ctgcttcgag caaagactcg aagattgccc
     1261 ggtccgaagg tctcgactcg attgagctgg cggcaactcc ggacatcctg gcatccgtag
     1321 tggctcgcgt cgatcggccg ttcgtcgtcg gttttgcagc cgaaaccggt ggtgtcgagc
     1381 gagcggtgga gaaagccaaa cgcaagtcgg tcgatttgct ggtctacaac gacgtatccg
     1441 agcccggatc aggattcgga accgatacca accgggtggt gatcatcggg cgtgacggga
     1501 gcaccgagga ctggccgctg cagtcgaagc gtcaggtcgc ggagcgactc tgggaccgga
     1561 ttggtgaaga agtacgggga gaggattaac ctcgctcccc gatgaggcgc tggtatttca
     1621 cttctgaatc ggtgaccgag ggtcatcccg acaaggtggc agacgccatc tcggacagcg
     1681 tgctcgacgc agcgctggag accacaccgg gagccagagt cgcatgcgag acctttgtca
     1741 ccactcgtct tgcagtagtt ggagg
//
LOCUS       DBNHXU010000303         3502 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_703476, whole genome shotgun sequence.
ACCESSION   DBNHXU010000303 DBNHXU010000000
VERSION     DBNHXU010000303.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3502)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3502
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_703476"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1030)
                     /gene="topA"
                     /locus_tag="AC161X_07575"
     CDS             complement(<1..1030)
                     /gene="topA"
                     /locus_tag="AC161X_07575"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003946104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HIR4989447.1"
                     /translation="MPKSLVIVESPTKARTIAAYLGADVSVESSMGHIRDLPRSAAEI
                     PAKAKGEDWARLGVDVEHDFEPLYVVPKERKDRVKRLKELLAGVDELYLATDEDREGE
                     SIAWHLTEVLKPKVPVHRMVFHEITKPAIEAAFANPRALDQRLVDAQEARRILDRLYG
                     YEVSPVLWRKIQQGLSAGRVQSVAVRIVVERERERMAFVAAGYWSIEGIFAPAENLER
                     TFDATLFQIDDKRVASGKDFDSSGRAKEGVLTLDQEQATTLAEALLNQPFNVASVEQK
                     PYKRSPYPPFRTSTLQQEAARKLRFSSSRTMSVAQRLYENGYITYMRTDSTALAESAV
                     ASARAQIAK"
     gene            1132..1728
                     /gene="tmk"
                     /locus_tag="AC161X_07580"
     CDS             1132..1728
                     /gene="tmk"
                     /locus_tag="AC161X_07580"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - dTMP kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006233 - dTDP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HIR4989448.1"
                     /translation="MRRYLALEGGDGSGKTTVASALVDRLEGRGHHVVLVREPGGTEL
                     GERVRDIVLDSDHVDRWAEVFLFAAQRAQLARDVVLPALERGAWVISDRSYYSSIAYQ
                     GRARGLGEARVRQINEIGLEGIEPDHVFVLEVDPSTALGRQKRPDRIGAESVEFQQQV
                     YEAYADLAREEPKRVMILSGDEPVESLVDRIMEVIEND"
     gene            1721..2758
                     /locus_tag="AC161X_07585"
     CDS             1721..2758
                     /locus_tag="AC161X_07585"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF024575.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003689 - DNA clamp loader activity
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HIR4989449.1"
                     /translation="MTDPFSGIIGHSRVIELLRRDAPEPAQAYLFVGPESVGKALLAK
                     QFAAAVLCSSQGVHDIECRSCRLVGAESHPDVSIVEPEGATSLGVDQIRSVVTRAALR
                     PVEGPRTVFLLPEAGSMTDQAANALLKTLEEPAAAVVFLLVAESEDDFPATVASRCRT
                     VHVGRVPLAELVAALEERGLGTDEATNVAVVSGGRPGLALALMNRPEVAQFRDLWLAI
                     PGRVTPRPGDGQRLAMEVLEEIGPLAERSVPAEVGKEAQQRARRRSEQSLLVNGLEIL
                     ASWYADSASLQMGGPVKNSDLSLAAFTDVNPRKAVMSAEHVLDAVVDIQGSLRRELVL
                     ATLFTGLGSQD"
     gene            2808..3287
                     /locus_tag="AC161X_07590"
     CDS             2808..3287
                     /locus_tag="AC161X_07590"
                     /inference="COORDINATES: protein motif:HMM:NF023075.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group I intron-associated PD-(D/E)XK
                     endonuclease"
                     /protein_id="HIR4989450.1"
                     /translation="MGITTAKGEIGEAIILADLQRQGHGVAIPFGHDLPYDLIVVRKE
                     DGALERVQCKYTTSNGKVVFAKITSTSAWVQHRYTSEEVDWIAVYDATTDICLYLPAS
                     VWNGQVCVNVRLAPTANGQVKRIRFVESFRALTGDPHATFWSGSAGTIPLPLGASPE"
     gene            3278..3349
                     /locus_tag="AC161X_07595"
     tRNA            3278..3349
                     /locus_tag="AC161X_07595"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3310..3312,aa:Thr,seq:cgt)
BASE COUNT          607 a         1040 c         1173 g          682 t
ORIGIN      
        1 gcttggcgat ctgcgcccgt gccgacgcca ccgccgactc ggcgagggcg gtcgagtcgg
       61 tccgcatata ggtgatgtag ccgttctcgt aaagacgctg cgcgaccgac atcgtgcgcg
      121 acgacgagaa acggagcttg cgcgccgcct cctgttggag cgtcgaagtc cggaagggcg
      181 gatagggcga tcgcttgtag ggcttctgct cgaccgacgc gacattgaac ggctgattga
      241 gcagtgcctc tgcgagggtc gtcgcctgct cctggtcgag ggtgaggacc ccttccttgg
      301 cccgccccga gctatcgaag tcctttccgc tggcgacccg cttgtcgtcg atttggaaga
      361 gagtggcgtc gaaggtgcgc tcgaggttct cggccggggc aaatatcccc tcgatgctcc
      421 agtagccggc ggcgacgaaa gccattcgct cccgctcccg ctcaacaacg atgcgcacgg
      481 cgacgctctg aacccttccc gcggagagac cctgctggat cttcctccac agcactgggg
      541 acacttcata cccgtagagg cgatccagga tgcgccttgc ctcctgggcg tcaacgaggc
      601 gttggtcgag tgctcgcgga ttggcgaacg ctgcctcgat cgccggtttg gtgatctcat
      661 ggaagaccat gcgatggacg gggacctttg gtttgagcac ctcggtcagg tgccacgcga
      721 tcgactcgcc ttcccggtcc tcgtcagtcg ctagatagag ctcgtcgaca ccggccagga
      781 gctctttgag cctcttgacc cggtctttgc gctctttggg gacgacatac aagggttcga
      841 agtcgtgttc gacatcgact ccaagacggg cccaatcctc gcccttggcc ttggccggga
      901 tctccgcggc cgagcgcgga aggtcccgaa tgtggcccat cgatgactcc acggagacat
      961 cggcgccgag gtaggcggcg atggtccgag ccttggttgg ggattcgacg atgacgaggc
     1021 tttttggcat tcgcggggtg agttgacccc aaaaggaagg gcaatgtcaa catatgcgct
     1081 tcgacaccag cccgttaccg tgcgagatgt gtgctcgctg atgggtgatt catgaggcgc
     1141 tatctagcgt tggaaggtgg cgatggctcg gggaagacca ctgtcgcctc cgccctcgtc
     1201 gaccgtctcg agggccgggg gcaccacgtg gtgttggtcc gtgagccggg tggcaccgag
     1261 cttggcgaga gggtccggga catcgtcctc gactcagatc acgtcgatcg gtgggccgaa
     1321 gtcttcctct tcgcagcgca gcgggcgcag ttggcgcggg atgtggtcct tccggcgctg
     1381 gagagaggag cctgggtcat cagcgaccgc agctactact catcgatcgc atatcaggga
     1441 cgggctcgcg ggcttggcga ggcccgggtt cgtcagatca atgagatcgg tctggaggga
     1501 attgagcccg accatgtctt tgtcctcgag gtcgatccga gcaccgcact cggtcgtcag
     1561 aagcgcccgg atcggatcgg cgccgaaagt gtggagttcc agcaacaggt ctacgaggca
     1621 tacgccgacc tggcgcggga agagccgaaa cgggtgatga tcttgagtgg agacgaaccg
     1681 gtcgagtcat tggtggaccg aatcatggag gtgatcgaga atgactgatc ccttctccgg
     1741 gatcattggc cactcccgtg tcatcgaatt gttgcgcagg gacgcccccg agccggccca
     1801 ggcatacctg ttcgttggcc cggagtcggt gggcaaagcc ctcctagcca agcagttcgc
     1861 ggcggcggtc ctctgctcct cccagggtgt ccatgacatc gaatgtcgct cctgtcggtt
     1921 ggtcggggcg gagagtcatc ccgatgtgtc gatagtggag ccggaggggg cgaccagtct
     1981 tggggtcgac caaatccggt cggtggtgac ccgtgcggcc ctgcgaccag tcgagggacc
     2041 ccgcacggtg ttccttctgc ccgaggcagg gtcgatgacc gatcaagcgg ccaacgcgct
     2101 gctcaagaca ttggaagagc ctgcggccgc ggtggtgttc ctgctggttg ccgaatcgga
     2161 ggacgacttc ccggcgacag tcgcatcccg ctgtcggacc gtgcacgtgg gccgtgtgcc
     2221 attggctgag ttggtggccg ccctcgagga gcgcggtctc ggaacagatg aggcgacgaa
     2281 tgtcgcggtg gtgtcgggcg gccggcctgg actggctctg gccctgatga accgtcctga
     2341 ggtggcgcag ttccgggatc tgtggttggc gatcccggga cgggtgacgc cccgccccgg
     2401 tgacggtcag cgcctggcga tggaggttct ggaggagatc ggcccactcg ccgagcgctc
     2461 ggttcctgcc gaagtcggca aggaagccca acagagggcc cgacggcggt cggagcagtc
     2521 gctgttggtg aacggtctgg agattctcgc ttcctggtac gccgactcgg cctctctgca
     2581 gatgggtggc ccggtgaaga actccgacct ctcactggcc gccttcactg acgtcaatcc
     2641 gaggaaagcg gtgatgtctg cggagcatgt gctcgatgcg gtggtcgaca tccaggggag
     2701 cctgcgtcga gaattggtac tcgccacact gttcaccggt ctcggctccc aggactgagc
     2761 cttttcgtcc cacccacccg atacgttctg aggtgggagg gaacaacatg ggcatcacca
     2821 ctgccaaggg cgagatcggg gaggcgatca ttctcgccga tctgcaacga cagggtcatg
     2881 gcgtcgccat ccccttcgga cacgacctgc catacgacct gatcgtggtc cgcaaggagg
     2941 acggcgctct ggagcgagtc cagtgcaagt acacgacttc gaacggcaag gttgtcttcg
     3001 cgaagatcac gagcacgtcg gcgtgggttc agcatcgcta caccagcgag gaggtggact
     3061 ggattgcggt ctacgacgcc acgactgaca tttgcctgta tttgcctgca agcgtctgga
     3121 acggtcaggt ctgtgtgaat gtccggctcg cacccacagc caacggccag gtcaagcgga
     3181 ttcgctttgt cgagtcattt cgtgctctga ccggggatcc ccacgcgact ttctggtctg
     3241 gctcagcggg aaccatcccc ttaccattgg gggcctcgcc ggagtagctc agtggtagag
     3301 caagcgcttc gtaagcgcta ggccaggggt tcgactcccc tctccggctc tggagaatgc
     3361 cggccgcctt cggccgtgtc gatgaaggaa aacagaagac tgttctgtac cgtcctcggg
     3421 gcaatggcgg accaagccga cttcgaacgc gaggcgttgc agtacacccg acaactgttc
     3481 agtgcggcca tgcgaatgac tc
//
LOCUS       DBNHXU010000304         8148 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5268759, whole genome shotgun sequence.
ACCESSION   DBNHXU010000304 DBNHXU010000000
VERSION     DBNHXU010000304.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 8148)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 8148)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8148
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5268759"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..405)
                     /locus_tag="AC161X_07600"
     CDS             complement(<1..405)
                     /locus_tag="AC161X_07600"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4989451.1"
                     /translation="MPTDTLPTGTVTFLFTDIEGSTRLVQALGARWVALLEDHNRLVR
                     AAIESNHGTVVKTEGDSFFAVFISALDAALASEAAQDALATHHWPEDGRVRARMGLHT
                     GIGALGGADYVGLDVHRAARIADAAHGGQTVLS"
     gene            complement(466..1467)
                     /locus_tag="AC161X_07605"
     CDS             complement(466..1467)
                     /locus_tag="AC161X_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006135026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03842 family LLM class F420-dependent
                     oxidoreductase"
                     /protein_id="HIR4989452.1"
                     /translation="MDFGVVLQTDPPASRVVELARMAEDLGFTHVWTFDSHILWQEPF
                     VIYSRILAETERVIVGPMVTNPGTRDWTVLASLFATLDDMYGPRTICGIGRGDSALRY
                     IGRQPRTLETMAEAMDVIKRLVAGDEIEYNGKMLSIPWAKPGWDLPMWAAGYGPKALD
                     IIGRHADGYILQLADPQILEWTSTAVRNAASDSGRDPSEVAMCVVAPAYVGDDLAHQR
                     DQLRWFGGMVGNHVVDMVNRYGEDAAKVPTVLSDYIKARESYDYQHHGRADNTSTDFV
                     PDDIVDRFCVLGPVEAHLEKLSLLESMGVNQFGIYLMHDDMEGTLRSYGQHVIGQLK"
     gene            1574..1903
                     /locus_tag="AC161X_07610"
     CDS             1574..1903
                     /locus_tag="AC161X_07610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989453.1"
                     /translation="MTTNQDSSGTPPVPRSVPIWWRAHDVIVGLLAGFGVGVIGGLFA
                     TRLIETNVIVAIGGAVGAILGIFALTLSRHREGGFVNATVVIAWVLLAGSALFLTALV
                     LAIANFE"
     gene            1887..2909
                     /locus_tag="AC161X_07615"
     CDS             1887..2909
                     /locus_tag="AC161X_07615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HIR4989454.1"
                     /translation="MPTSSSSAHPHRLLLAAACVLLVAGCSSEVAPRQADRETTPGLV
                     VVPLDSPGTVAYVARGTVSEEFFFNADVGVEDREGVAAPISGTLIGPLPQRGTVVVAG
                     DVLFVMAPTLEMQAAAAELEAALLALEMGNGDQADLEARVAAASQVAEELGLPTDEQA
                     LQPLPKEFVTEAPTSGTVLETHPPIEGIAAQGDVLIELGDTEDLVVNVSVPPDIAELV
                     EVGSPVLVATRDGRGEPVNGVVASIQEPEEGAEADAEVTITVELETDAFDYGTRVRVA
                     ITGVSHEDVLWLPPEVIRSHDGQTFVIVEDEDDQKRVNVLLGAQTEEQVEVRGLLYEG
                     DKVIGP"
     gene            2906..5653
                     /locus_tag="AC161X_07620"
     CDS             2906..5653
                     /locus_tag="AC161X_07620"
                     /inference="COORDINATES: protein motif:HMM:NF014717.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989455.1"
                     /translation="MTSLKLAFIRLKARPVLSILLLIGLATAITLPMAIPIYSDAATA
                     RVLSQQDFQDIGYRPPFAYLFFYSSELEEPVSWGQVAEVDSYLRNEGLEQLGLPIDYA
                     TGFVDTRLFDLRQGTDEIGLGSVSMAALDDFAERANMVSGRLPEVRVDGDPIEIVIGL
                     DLAEETGLEVGDIVQAYDPEAELTDPYQTIPVEVVGLWEPRDPADPQWIISPDQLVNR
                     VFVDRAVITEQLSPIKADLIRTAAWYAVMDGSSLTAGDVVPLINNAETVLAEAAQLLP
                     GISRVIDPIDGLHEFQSARDALARRLAAYALPTFGLVLVFLMLAVSLTTNERRSELAV
                     LRSRGASRAQLVGQVGVESALIAAGAYLLAIPLSLGVASLMGSVDTFLGFGARDPLPV
                     QLTPPALKTGLIVALASLILQVLPVWGAARSTVVTHDEMAMQATRRPWWQRSSLDLIV
                     VVVVVVVGYQLLSNEPELGSSLSDPTIILLPALVTLAVGLILLRLLPPVLEVLARGLR
                     YTPSVTWLVALRRAARSPGSSHVPLLLLVVTIGLAIFTASLAETLDLQLVDEAYHQVG
                     ADWSVYEADSAAEPSIRGVPAGPRELASIEDFEAIPGIDRATRVAKFTGRLSTASRPV
                     ISVAFYGIDRDSFPEVAFFRSDYANSGTVPDAIAALGSAPEAILVHPSLGLNKGEVVD
                     ADISFEGSTLHTRFVVAGTLDQFPTWYPEEDAPLVVGNLDQVFLQTGQPALYRVWMSN
                     DDPPAEEIESDISLIETVSPIPQIQETMTAPARQGVFGLLTIGFIGAVFLSLMGFFFA
                     TMFRVRMSAVELGALQALGMPPRRVAGVVVVELGILMSFGLAAGVLTGWQLSRWLIVR
                     LVGDSGLIATPPLLAEIDEAAIWGVIVLLIGLFLVTASALVLVLRRMRVFEALKLGEN
                     A"
     gene            5650..6531
                     /locus_tag="AC161X_07625"
     CDS             5650..6531
                     /locus_tag="AC161X_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015254322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4989456.1"
                     /translation="MIEVDPQIPGSVIQCERVVKIYRVGSTDVPALAGLDLRVDRGEM
                     VGVIGPSGCGKSTLLNVIGALISPTSGVVIVDGEDLAAMSQTELDKFRRERVGFVWQD
                     TARNLLPYLDARANVALPLRLAGTDHAAYRAQELLELVGLGDRALHVPSRLSGGQQQR
                     VAIAVALANSPSLLLADEPTGALDGETSHEIYETLREVNRELGITVFIVSHDPNMAAV
                     VDRVVELRDGQSAMEHRGSGRNEDGSVVLLVDSLGRIRVPDEFRESLGIGDRLEARLD
                     DDQVTLTPIDPPRKDHR"
     gene            6528..7226
                     /locus_tag="AC161X_07630"
     CDS             6528..7226
                     /locus_tag="AC161X_07630"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989457.1"
                     /translation="MIPAPENLAVQVHAVERIYDGPEGPVIALSGVDLTVAPGTMVAV
                     RGRSGSGKTTLLNLVGALDQPTAGEVHVFGRRIDQLTPGAAASWRAETLGFVFQAHAL
                     MPAMSALENVDLGLRIAKTERKEAERLARESLERVGLGDRLDHRPSELSGGQQQRVAV
                     ARAIATGNRLVLADEPTAGLDSSAASEVFGLFRSLVDDSGITILMATHDPLTEEYVDA
                     ATHLEGGRLVIDRI"
     gene            complement(7234..7626)
                     /locus_tag="AC161X_07635"
     CDS             complement(7234..7626)
                     /locus_tag="AC161X_07635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989458.1"
                     /translation="MLVATAILATACSLSVQVGATTTTLRGEGFTLADLVGEWENREL
                     VMQVGDDGQYVVHEAANEEPSDPLMSGFVARDGEELNFVTDIYGECAGETGVYEVLMS
                     EGQLILILVDDPCEFRSDRFVDPWESSG"
     gene            complement(7657..>8148)
                     /locus_tag="AC161X_07640"
     CDS             complement(7657..>8148)
                     /locus_tag="AC161X_07640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4989459.1"
                     /translation="APAGDATQQTSDLMTALSEAGLADRQIVYVAHGEGVADAQAFVA
                     QFSEQVDGLVLVDPAPAEYAKYADDVTDRFPYEGSGDFGGRPLVVIGHDLDVTIRSAA
                     FIASQGEAKSEEVSTIWQQALDGYAALSTNSTQLTADGSNHAVPWERPQMIIDAVNGL
                     FDS"
BASE COUNT         1427 a         2590 c         2575 g         1556 t
ORIGIN      
        1 ggagagcacc gtctgcccgc catgcgccgc atccgcaatg cgggcggccc gatgcacgtc
       61 gaggccgaca tagtcggccc cacccagggc cccgataccg gtgtgcagcc ccattcgggc
      121 ccgaacccga ccgtcctcgg gccagtggtg ggttgccagc gcatcctggg cggcttcgct
      181 ggcgagagcg gcgtccaggg cagagatgaa gacggcgaaa aaggagtcgc cctctgtctt
      241 gaccacagtg ccgtggttgg attcgatcgc cgccctgacc agtctgttgt gatcttcgag
      301 gagggcaacc caacgtgctc ccaaggcttg gacgaggcgg gtcgagcctt cgatgtcggt
      361 gaagaggaag gtaacggtgc cggtcggaag ggtgtcagta ggcattgccg tagtggttag
      421 tgatccacac cagacctcaa cattaggtgc gcccgagtcc ggcgattact tcagctgacc
      481 gatgacatgc tggccataag agcggagagt gccctccatg tcatcgtgca tgaggtagat
      541 cccgaactga ttgaccccca tcgactcgag aagggacagt ttctcgaggt gggcctcgac
      601 gggccccaga acacagaacc tgtccacgat gtcatcggga acgaaatcgg tggaggtgtt
      661 gtcggcccgg ccgtggtgtt ggtagtcata tgactcgcgg gccttgatgt agtccgagag
      721 aacggttggc accttggccg catcctctcc ataacggttg accatgtcca caacgtggtt
      781 cccaaccatt ccgccgaacc agcgcagttg gtcacgctgg tgggccagat cgtctccgac
      841 ataggccggg gcaacgacac acatcgccac ctctgacggg tcgcgtcccg aatcgcttgc
      901 agcgttgcga acggctgtcg aggtccactc caggatctga gggtccgcca gttgaaggat
      961 gtagccgtcc gcgtggcgac cgatgatgtc cagcgccttg ggcccgtagc cggcggccca
     1021 catcgggaga tcccatcccg gcttcgccca aggaatcgat agcattttgc cgttgtactc
     1081 gatctcatcg ccagccacga ggcgcttgat gacatccatt gcctcggcca ttgtctcgag
     1141 cgtcctcggt tggcgcccga tgtagcgcaa agcagagtcg ccccgcccga tgccgcaaat
     1201 ggtccgcggc ccgtacatgt cgtcgagagt tgcgaacaac gaggccagca ccgtccagtc
     1261 cctggtgccg gggttggtga ccatagggcc gacgatcacg cgctcggtct cggcgaggat
     1321 ccgcgaatag atgacgaatg gctcttgcca caggatgtgg gagtcaaagg tccagacatg
     1381 ggtgaagccg agatcctcgg ccatcctggc cagttccacc acccgcgaag ccggtgggtc
     1441 ggtttgaagc acgacaccga aatccatcag ctgacgtcct gagtcatggg ttcaagttag
     1501 ctttagaccc ggccaggcga ggactctccg ttgtcgccga gaaaacggca taatcgccgc
     1561 cggcgctcac ctcatgacca cgaaccaaga ctcttcgggg actcctccgg tccctcgttc
     1621 cgtcccgatc tggtggcggg cccacgacgt catcgtcggc ctgctcgccg gcttcggcgt
     1681 gggtgtcatc ggagggctgt tcgccaccag actcatcgag accaatgtga tcgtggcgat
     1741 aggcggagca gtcggagcca tccttggcat cttcgctttg acactcagtc gtcaccgtga
     1801 aggcggtttc gtcaacgcca cagtcgtgat cgcctgggtc ctcttggccg gctccgcgct
     1861 attcctcaca gcactggtgc tggccattgc caacttcgag tagctcagcc catccccatc
     1921 gcctgctgct cgccgctgcc tgtgttctcc tggtggcagg ttgcagcagc gaagtcgcac
     1981 cgcgccaggc ggatcgcgag accacccccg gcctcgtggt tgtacctctg gactcgcccg
     2041 gcacagtggc atatgtggcc cgaggcaccg tctctgagga gttcttcttc aacgccgacg
     2101 tgggcgttga agaccgggag ggcgtcgccg ccccaatctc agggactctc attgggccac
     2161 ttccacaacg aggcaccgtg gtcgtggccg gagatgtcct gtttgtcatg gctccgaccc
     2221 tcgaaatgca ggctgcggcc gcagaactgg aggcggcgct cctcgccctc gagatgggca
     2281 atggcgacca ggcggacttg gaggcaagag tcgctgcggc aagccaagtt gccgaggagc
     2341 tcggcctacc caccgacgaa caggcgttgc aaccactccc caaggagttc gtaactgagg
     2401 ctcccacatc cgggaccgtc ctcgaaaccc atcctcctat cgagggcatt gccgcccagg
     2461 gcgacgtcct catcgaactt ggcgacaccg aggaccttgt ggtgaacgtg agtgtccctc
     2521 cggatattgc ggaattggtc gaagttggat ccccggttct cgtcgccacc agggatgggc
     2581 gcggcgagcc ggtcaatgga gtggtcgcct cgatccagga acctgaggag ggggcagagg
     2641 ccgatgccga ggtgacgatc accgttgagc tcgagaccga tgccttcgat tacggaacca
     2701 gggtccgagt ggcaatcact ggtgtgtccc acgaggacgt cctctggctg ccgcccgaag
     2761 tcatccgctc tcacgatggt caaaccttcg tgatcgtcga ggacgaagac gaccagaaaa
     2821 gggtcaatgt cttgctcgga gctcagaccg aggagcaggt agaggtccgc gggctgctct
     2881 atgagggcga caaggtgatc ggcccatgac ctcgctgaag ctcgcgttca tccggctgaa
     2941 ggcgagaccg gtcctctcga tcctgctcct aatcggcctg gccacggcca tcaccctccc
     3001 gatggccata ccgatctact cggatgccgc aacggcaagg gtgctttctc agcaggattt
     3061 ccaggacatc ggctaccggc cgccgttcgc ctatctcttc ttctactcga gcgagctcga
     3121 agagccggtg agttggggcc aggttgccga ggtggacagc tacctccgca acgaaggcct
     3181 cgagcaactc ggccttccaa tcgactacgc cacgggtttt gtcgacaccc ggctcttcga
     3241 cttgcgccag gggaccgacg agatcggctt ggggagcgtt tcgatggccg ccctggacga
     3301 cttcgccgag cgggcgaaca tggtctccgg gcggttgccc gaggtccggg tggatgggga
     3361 tccgatcgag atcgtgatcg gccttgacct ggctgaggag accggcctgg aggtgggcga
     3421 catcgttcag gcgtacgacc ccgaggccga gttgaccgac ccctatcaga cgattccagt
     3481 cgaggttgtg ggtctgtggg agccgaggga cccggcggac ccacagtgga tcatctcccc
     3541 cgaccaactc gtcaaccggg tgttcgtcga tcgagctgtg atcaccgagc aactttcgcc
     3601 gatcaaggcc gacctgatcc gaactgccgc gtggtatgcc gtcatggatg gttcgtccct
     3661 gaccgcaggc gatgtcgttc ccctgatcaa caatgccgag accgtcctcg ccgaggcggc
     3721 gcaactgctg ccggggatct cgagggtcat cgatccaatt gatggcctcc acgaattcca
     3781 gagcgccaga gatgcgctgg cgaggaggct ggccgcatac gccctaccca ccttcggact
     3841 ggtgctcgtg ttcttgatgc tggcggtgtc cctgactacc aacgagcggc ggtccgaact
     3901 ggcggtgctc cgcagccgcg gggcgagccg cgcccagctc gtgggccagg tcggggtcga
     3961 atctgccctg atcgccgccg gcgcctatct gctcgccatc cctttgagtc tcggagtggc
     4021 atcgctgatg ggaagcgtcg acaccttcct cggcttcgga gcaagggacc cattgccggt
     4081 ccaactgacc ccgccggccc tcaagaccgg cctgattgtg gccctggcca gcctcatcct
     4141 tcaggtgctt ccggtgtggg gggccgccag atcgacggtg gtgactcatg acgagatggc
     4201 catgcaggcg acaaggcggc cgtggtggca gagaagctcg ctcgacctga tcgttgtggt
     4261 ggtggtggtc gtagtcggct atcagctgct gagcaatgag cccgagctcg gatcgtctct
     4321 ctccgacccc acgatcattc tgctcccggc gctggtgact ctcgccgttg ggttgattct
     4381 cctccgactt ctgccgccgg tcctcgaagt cctggcccgt ggcctccgct acacgccgag
     4441 cgtcacctgg ctggtggcac tgcgccgggc cgcccgcagc ccgggaagca gccacgtgcc
     4501 cctgctcctt ttggtggtca ccattggatt ggccatcttc accgcctctc tggccgagac
     4561 cctcgacctt caactcgtcg atgaggccta tcaccaggtc ggcgccgact ggagtgtcta
     4621 tgaggccgac tcggctgccg agccgtcgat ccgcggcgtg cccgcggggc cgcgagagct
     4681 cgcttctatc gaggatttcg aggcgatccc tggtatcgac cgcgccaccc gggtggcgaa
     4741 attcacaggg cgcctctcga cggccagccg ccctgtgatt tcggtcgcct tctatggcat
     4801 cgacagagac agcttcccgg aggtggcctt cttccggtcc gactatgcga acagcggaac
     4861 ggtccctgac gcaatcgccg ctctggggtc ggcccccgaa gcgatcctgg ttcacccgtc
     4921 cctcggcctg aacaagggcg aggtcgtcga tgccgatatc tcgttcgaag ggtccacctt
     4981 gcacacccga tttgtggtgg cgggcaccct cgatcaattc ccaacctggt acccggagga
     5041 ggacgcgccg cttgtggtcg gcaacctcga ccaggttttc ctccagaccg gccaaccagc
     5101 tctgtatcgg gtttggatgt ccaacgacga ccccccagca gaggagatcg aatcggacat
     5161 atccctgatc gagacggtga gcccgatccc acagatccag gagacgatga ccgctcccgc
     5221 tcgtcaggga gtcttcgggc tgctcaccat cggcttcatc ggagctgtgt tcctatctct
     5281 catgggcttc ttcttcgcca cgatgttccg ggtgaggatg tcggccgtcg agcttggcgc
     5341 gctccaagcg ctgggaatgc ctccccgccg ggtcgccgga gtggtggtgg tcgagctcgg
     5401 gatcctgatg tcattcggtt tggcggcggg tgtgcttacg ggctggcaac tgagccggtg
     5461 gctcatcgtg cgtttggttg gtgactccgg cctgattgcc accccgccgc tcctcgcaga
     5521 gatcgacgag gctgcgatat ggggtgtcat cgttctcctc attgggctct tcctcgtcac
     5581 cgccagcgca ctggtgctgg tattgcgacg catgagagtc ttcgaagccc tcaagctggg
     5641 agagaacgca tgatcgaagt cgatccgcag atccccggct cggtgatcca gtgcgagcgg
     5701 gtggtaaaga tctaccgggt gggatctacc gatgtgccgg cgctggcagg gctggacctt
     5761 cgcgtcgatc gcggagagat ggttggtgtg atcggtccca gcggctgcgg caagtcgacc
     5821 ctgctcaacg tcattggagc cctgatctcg cctacgagcg gtgtggtcat tgtcgatggg
     5881 gaagacctgg ccgcgatgag tcagaccgaa ctcgacaaat ttcgccggga gcgggtgggg
     5941 tttgtctggc aggacaccgc acgcaacctg ctgccctatc tcgatgccag agccaatgtc
     6001 gcgttgccgc tccgtttggc cggaaccgac catgccgcct atcgggcaca agagctcttg
     6061 gaactggtcg gactcggtga tcgggccctc cacgttccca gccgattgtc cggcggtcag
     6121 cagcaacgcg tcgccatcgc cgtggcactt gccaactccc cttcgctgct cctcgccgac
     6181 gagccgaccg gggccctgga cggagagacg tcacatgaga tctatgagac gttgcgcgag
     6241 gtgaatcgag aattgggcat caccgtgttc atcgtgagcc atgacccgaa catggcggcg
     6301 gttgtcgacc gtgtggtcga actgcgggac gggcagagcg ccatggagca tcgaggatcg
     6361 ggccggaacg aggacggatc ggtagtgctt ctggtcgaca gtctgggcag gatccgcgtt
     6421 cccgatgagt tccgggagag tcttggcatc ggtgacaggc tcgaggcccg gctcgacgac
     6481 gatcaggtga ccctgacccc aatcgatccg ccacgcaagg accatcgatg atccccgccc
     6541 ccgagaacct cgcggtacaa gtccatgcag tggaaaggat ctacgacggg ccggaaggac
     6601 ccgtgatcgc cctcagcgga gtcgacctaa ccgtggctcc gggaaccatg gtcgcggtgc
     6661 ggggcagatc tggcagcggg aagaccactt tgctcaatct ggtcggcgcc ctggatcagc
     6721 ccacggcggg cgaagtccat gtgttcgggc gtcggatcga ccaactgacc ccgggcgcgg
     6781 cggcgtcttg gcgagccgag actctcggat tcgttttcca ggcccatgcc ctcatgccgg
     6841 cgatgtctgc attggagaac gtcgatctcg gcctgcgtat cgccaagacc gagcggaagg
     6901 aggccgagcg cctggcccgg gagagcctgg agcgagtagg tctcggggat cgactcgatc
     6961 atcggccttc ggagctctct gggggtcagc agcagcgggt cgccgttgcc cgggccatcg
     7021 ccactggaaa ccggctggtc ctcgccgacg agcccactgc cggcctcgat tcgtcagctg
     7081 caagcgaagt ttttggcttg ttccggtctc tcgtcgacga ctccgggatc acgatcctca
     7141 tggccaccca cgatccgctc actgaggagt acgtggacgc ggcgacccat ctcgaaggtg
     7201 gtcgactggt gatcgaccga atctgaggtc agatcagccc gagctctccc acggatccac
     7261 gaatcggtcg gatcgaaact cacagggatc gtcgacgagg ataaggatca gctgtccttc
     7321 gctcatcagc acctcgtaga cgccggtctc cccagcgcac tccccgtaga tatccgtgac
     7381 gaaattcagc tcttccccat cgcgggccac gaagccactc atcagtgggt cactcggctc
     7441 ctcgttggcg gcctcgtgga ccacgtactg gccgtcgtcg cccacttgca taaccaactc
     7501 gcggttctcc cactcgccaa ccagatcggc caaggtaaat ccctcgcctc tgagcgttgt
     7561 agtcgttgcg cccacctgga cagagagcga gcaagctgtg gcgaggatgg ccgtcgcaac
     7621 caacatcagc agtcgccctc gatgcaccac gcgatgttag gagtcgaaca gcccgttcac
     7681 cgcgtcgatg atcatctgcg ggcgctccca gggaactgca tggttgcttc catcggcggt
     7741 cagttgggtt gagttggtgg agagggcggc atacccgtcc agcgcctgct gccagatggt
     7801 gctcacctct tccgacttcg cttcaccctg gctggcaatg aacgccgcgc tgcggatagt
     7861 cacgtcaagg tcgtgcccga tgaccaccaa tggacgacct ccaaagtcgc ccgagccctc
     7921 gtaggggaag cgatccgtga cgtcgtcggc gtacttggcg tactcggcgg gggcaggatc
     7981 aaccaggacc aggccatcga cctgctcgct gaattgggca acgaacgcct gcgcgtccgc
     8041 cactccttcc ccgtgggcaa catagacgat ctggcggtcg gcaagcccgg cctcactgag
     8101 ggcggtcatc agatcagagg tctgctgggt ggcgtcgccg gccggtgc
//
LOCUS       DBNHXU010000305         5825 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4672215, whole genome shotgun sequence.
ACCESSION   DBNHXU010000305 DBNHXU010000000
VERSION     DBNHXU010000305.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5825)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5825)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5825
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4672215"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(177..1688)
                     /locus_tag="AC161X_07645"
     CDS             complement(177..1688)
                     /locus_tag="AC161X_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006367415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008519 - ammonium channel activity
                     [Evidence IEA];
                     GO_process: GO:0072488 - ammonium transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HIR4989460.1"
                     /translation="MRPKRRVRKLATLASLIGLMVVSMAAPAIAQDADPVTTMGQQLN
                     LLWIVIGAVLVIFMQAGFALVETGFSRAKHAAHVVSTNFAIFGIGFVAYFLVGYAFMF
                     GGFSYVLPGFDFGYDTALGGPLIGSGDWTFLWQGGFALQGAITPAVLGFFLYMAAFMD
                     TTATIPTGSMAERWKWRSFVGWGLFCGAIYYPLFGAWTWGGGWLNKLWSSMGWGVGYA
                     DFAGSGIVHAVGGAAALAGALVLGPRIGKFRADGKANTLPGHHIPMAALGTFILLFGW
                     FGFNAASTFAATDVQFATVAANTAIAGAFGAVISMLYCMYFRGKKPDPGMMMNGMLAG
                     LVAITAPCAFVTPGWSAVIGIIAGVLVVEAILFIEKRGIDDVVGAIAVHGVCGTFGVL
                     AVGIFPDGSYGAGWNGAVDSAGEPVEMLGLIYGGTGQFLAQLAGVVVIWTVMFGLAYT
                     FFKIQNSLTKGGIRVPEEEELLGVDIPEMGVLAYPEFTARAIEADEEPASSSV"
     gene            complement(1799..2314)
                     /locus_tag="AC161X_07650"
     CDS             complement(1799..2314)
                     /locus_tag="AC161X_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4989461.1"
                     /translation="MDVAFIRWPGEKEKRASMLASQQPHLLLVESGPPPVDVTLIEDW
                     IRIPAPAVDIEARLELLRRRSIPAQPSRLTPFLGADGILRFGDDWVSLSPLEVRLVSA
                     LLARPDAVVTRQALIDSGWPQVGEGRPNPRRNTLDAHMVRLRQRLPAVGLTVRTIRSR
                     GYMLEQLAASL"
     gene            2430..3590
                     /locus_tag="AC161X_07655"
     CDS             2430..3590
                     /locus_tag="AC161X_07655"
                     /EC_number="3.5.1.28"
                     /inference="COORDINATES: protein motif:HMM:NF013661.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008745 - N-acetylmuramoyl-L-alanine
                     amidase activity [Evidence IEA];
                     GO_function: GO:0016019 - peptidoglycan immune receptor
                     activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0045087 - innate immune response [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein family
                     protein"
                     /protein_id="HIR4989462.1"
                     /translation="MHAGLIALTATLALALLPPGGTFIDDDGNVHEGGIEAIAAEAIT
                     TGCNPPSNNRYCPSDHVTRGQMAAFLTRALHLPSAPQDHFSDDAGTVFENAINSVASA
                     DITLGCNPPENSRFCPDREMTRGEMAAMLTRAFEYPATAKNWFNDDNGHLFEGAINRM
                     AAAGVTLGCNPPANDHYCPDQLVARDQMATFLTRALGLTANRPPPRPVPVVSVVSREE
                     WGARQPQPDRMVPHTITRLTVHHAGDQVGATGPAQFRGWQDWHMSGQGWPDIAYHVLI
                     GVDGKVYEGRDPAYRGDTGTSYDTTGHFLVVVEGNFEVEHPTAAQLDSLVKVLAWAAE
                     HYGVSTSTIAGHRDYAATSCPGKYLAQKIDSGEVQADVEGLLAKGGVDLIWP"
     gene            complement(3609..4247)
                     /locus_tag="AC161X_07660"
     CDS             complement(3609..4247)
                     /locus_tag="AC161X_07660"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:TIGR02228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR4989463.1"
                     /translation="MTRVAVSAPVISPATKRAAPSVRTVMVWLLTLILAAGWFAFLRP
                     TIWGGPLTYVVIRGTSMRPTYQPGDLVLVRREANYGPGDIVAYRVPNDDIASDLILIH
                     RIIGGSGEEGFTLLGDNNPEEDIWYPTNSDIVGRPVFHIPAVGLILTFLRAPLVLASL
                     AAGATVAIVLVPKSSDGGPAQASTPESSGRHVRRRRIWRLRLTIRHGAHVEG"
     gene            complement(4244..4924)
                     /locus_tag="AC161X_07665"
     CDS             complement(4244..4924)
                     /locus_tag="AC161X_07665"
                     /inference="COORDINATES: protein motif:HMM:NF048382.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNRLRE domain-containing protein"
                     /protein_id="HIR4989464.1"
                     /translation="MIGVLVLALLPTAAAAAPITLTAKTLAVYRTCTLTAWAAGSTTV
                     ADSWVNHNNLTQNNGTATFMDVQTAGATTNRRAYIRFDLTQCSPAIPSSATISSGTLR
                     LYASAIPGVCRTQDIFRVTFAWTEAGLTWNTQPAGWNAANQPASGSRTSFITVGPGCQ
                     NTAVGYVNGWDVTADVSAFVAGTATNHGWMIRDDIEASGTARNVRYSSKDLANLAQAP
                     QLTVTYRP"
     gene            complement(4962..5336)
                     /locus_tag="AC161X_07670"
     CDS             complement(4962..5336)
                     /locus_tag="AC161X_07670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989465.1"
                     /translation="MRRWLIGMVAAFVTIVATIAPGLAANLPSSSQSLAGANVAVASC
                     DTDGVTVVRTLSGANVVSVTISNIASACGNGALSMTLSNGSTSVSATAVVPAGGGSVV
                     VTPATPIAATNAMTTHLTIVGA"
     gene            complement(5359..5748)
                     /locus_tag="AC161X_07675"
     CDS             complement(5359..5748)
                     /locus_tag="AC161X_07675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989466.1"
                     /translation="MKRILIALVVAGAVFGAVLGLAASLTVNTETLGAGSSTVAACQA
                     TPLTVTYATSYVAGSSYRVGVVTVTGMAAGCLTKPYRITLAGTSNASLGEVTGTTPGS
                     GTSFTADFSSANVSAASVLGVHLVISG"
BASE COUNT         1162 a         1838 c         1825 g         1000 t
ORIGIN      
        1 gtcaggccga gtccaacacc gaatcggggc acagacccga gctgtcgccc acggagatga
       61 gggctgtggg ttcggtggtg cccgctgggg gtgggcgcaa gatctggccc gcgtgatcgc
      121 gagcaaaatg ggggtcggca ccaggtggtc ccgaccccca tttcgcatta gctcggtcag
      181 accgaactgg aagctggttc ctcgtcggcc tcgatcgccc gagcggtgaa ttctgggtaa
      241 gcgagaacac ccatctccgg tatgtcgacg cccaacaact cttcctcttc gggcaccctg
      301 atcccaccct tggtcaagct gttctggatc ttgaagaagg tgtaggcaag cccgaacatc
      361 acggtccaga tcacgaccac cccggccagc tgggccagga actggccggt gccgccgtag
      421 atcagcccga gcatctccac cggctcgcca gcgctgtcga cggcaccatt ccaaccagca
      481 ccgtaactgc cgtcggggaa gatgccgact gcgagcaccc cgaaggtgcc acagacacca
      541 tggactgcaa tggctccgac cacgtcatca atacccctct tctcgatgaa gagaatcgcc
      601 tcgaccacta gaacaccggc gatgatcccg atcactgccg accagcctgg agtgacgaac
      661 gcacagggag ccgtgatcgc caccagcccg gccaacatgc cgttcatcat catcccggga
      721 tcgggcttct tgcctctgaa atacatgcag tagagcatcg agatgaccgc cccgaaggcg
      781 ccggcgatcg ccgtgttggc ggccaccgtg gcgaattgga cgtcagttgc agcaaaggtg
      841 gacgcggcgt tgaacccgaa ccatccgaag agaaggatga aggttcccaa ggccgccatt
      901 gggatgtggt ggccgggcag ggtgtttgcc ttcccgtcgg cccggaactt gccgatccgg
      961 ggtccaagta cgagtgcgcc ggccaaggcg gccgcgccgc cgacggcgtg cactatgccg
     1021 gacccggcga agtcggcgta accgacaccc cagcccatcg agctccacag tttgttcaac
     1081 caaccgccgc cccaggtcca ggcgccgaag agcgggtaat agatcgcccc gcagaagaga
     1141 ccccagccga caaagctcct ccatttccaa cgctccgcca tcgaaccggt ggggatggtg
     1201 gcggtggtgt ccataaacgc tgccatgtag aggaagaacc cgagcactgc tggtgtgatc
     1261 gcaccctgca gtgcgaaacc gccctgccag aggaaggtcc agtcgccaga tccgatcagc
     1321 gggccgccca gcgcggtgtc gtacccgaag tcgaagccgg ggagcacata ggagaaccca
     1381 ccgaacatga aggcataacc gaccaggaag taggcaacga acccaatccc gaagatggca
     1441 aagttggtcg aaaccacatg cgctgcgtgc ttggccctgg aaaacccggt ctccaccagg
     1501 gcaaacccgg cctgcatgaa gatgaccaga accgccccga tcacgatcca caggagattg
     1561 agctgctgac ccatagtggt caccgggtcg gcatcctggg ctatggccgg tgcagccatg
     1621 cttacaacca tcaagccaat caggctggcg agggtcgcca acttcctgac ccggcgtttc
     1681 ggtctcatcg aaggtcctcc cttatctggt tgagctggtg tcaacgagcg taagaaaggg
     1741 ctgttaatcg tcttgaaacg cagtattacg agtctggatc agtcagatga cagctttctt
     1801 acaagctggc ggcgagctgc tccagcatgt atccgcggga tctgatggtc ctcactgtga
     1861 gacccactgc cggaagtcgc tggcgaagac gaaccatgtg ggcgtccaaa gtattgcgcc
     1921 tcggattggg ccggccctcc ccgacctgcg gccatcccga atcgatgagg gcctgacggg
     1981 tgacgaccgc atcgggtcgt gccagcagag ccgacaccag acggacctcg agaggcgata
     2041 gggacaccca gtcgtcacca aatcgcagga tgccgtcggc cccgaggaat ggcgtgaggc
     2101 gggacggctg ggccgggatc gacctgcggc gcagcaactc gagacgggct tctatatcga
     2161 ccgccggagc ggggattcgg atccaatcct cgatgagggt gacgtcgaca ggcggcggcc
     2221 cggattcgac caggagcaag tgaggttgtt gggaggccag catcgaggct cgcttctcct
     2281 tttcgccagg ccaacgaatg aaagccacat ccacgcaaca acgttctcag agtcgtgttt
     2341 caaacgtgtt tccccgatgt gaagggtgca cgacaccccg ctgagctggt atcgatggtt
     2401 accctcatcg tccgtcgatc ggagaaggga tgcacgcagg actgatcgcc ctcaccgcga
     2461 ccctcgccct ggcgctgctg cccccagggg gcactttcat cgacgatgac gggaatgtcc
     2521 atgagggtgg catcgaagcc atcgccgccg aagccatcac gaccggttgc aatcctccta
     2581 gcaacaaccg ctattgcccc agcgaccatg taacccgtgg ccagatggcc gccttcctca
     2641 cgcgagcgct tcacctgcct tctgctccac aggatcactt ctcagacgac gccggcactg
     2701 tcttcgagaa cgccatcaac tcagtcgcct ccgccgacat caccctcggg tgcaacccgc
     2761 ccgagaacag tcggttctgt ccggacaggg agatgacccg cggtgagatg gcggcgatgt
     2821 tgacgcgcgc tttcgaatat ccggcgacgg ctaaaaactg gttcaacgac gacaacgggc
     2881 acttgttcga gggggcgatc aatcggatgg ctgcagccgg ggtcaccctc gggtgcaacc
     2941 cgccggcaaa cgaccactac tgcccggacc agttggtagc acgtgaccaa atggccacat
     3001 tcctgaccag ggcactcggg ctcaccgcca accgcccacc gccccgcccg gtgccagtcg
     3061 tctccgtggt ctcccgggaa gagtggggag cccgccaacc ccagccagat cgcatggtcc
     3121 cccacaccat tacgaggctg accgtccatc acgcgggtga ccaagtgggt gcgaccggcc
     3181 cggcccagtt ccggggttgg caggattggc acatgtccgg ccaggggtgg cctgacatcg
     3241 cctaccacgt gctgatcgga gtcgacggga aggtgtacga aggtcgagac cctgcctatc
     3301 ggggagacac cgggaccagt tatgacacca ccggccattt cctggttgtg gtcgagggca
     3361 atttcgaggt agagcatccc actgccgccc agctcgactc cctggtcaaa gtgctcgcct
     3421 gggccgccga gcactacggc gtgtcaacgt cgaccatcgc cgggcaccgg gattacgcgg
     3481 ccacctcgtg ccctggcaag tacctcgcgc agaagatcga ttcaggggag gtccaggccg
     3541 atgtcgaggg cctccttgcc aaaggtggcg tcgacctcat ctggccctga gggtctccga
     3601 gtccgcgatc agccttcgac gtgtgccccg tggcggatgg tcagtctcag tctccagatc
     3661 cggcgtcggc gaacatggcg gcccgaggac tcgggcgtcg acgcctgcgc tggtccaccg
     3721 tccgaggatt tgggcaccaa cacgattgcg accgtggctc ccgcggcaag gctcgccaat
     3781 accaacggag cccgaaggaa ggtcaagatc agcccgactg cgggaatgtg aaacacgggt
     3841 cgaccgacga tgtctgagtt cgtcggatac cagatgtctt cttctgggtt gttgtctccg
     3901 agaagagtga atccttcctc acctgaccct ccaatgatcc ggtggatcaa gatcagatcg
     3961 gaggcgatgt catcgttggg cacgcgatag gcgacgatgt cccccggccc atagttggcc
     4021 tctcgccgca ccagcaccag gtcgcccggt tgataagtgg ggcgcatgct ggttcctcgg
     4081 attacgacat aggtgagtgg ccctccccag atcgtcggcc gcaggaaggc aaaccaccct
     4141 gctgcgagga tcaaagtcaa cagccacacc atgaccgtcc gtaccgatgg ggcagctcgt
     4201 ttggtcgcag gagagatgac aggcgcggag acagcgaccc tggtcatggg cggtaggtca
     4261 cggtgagttg gggtgcttgg gccaggttgg cgaggtcctt cgacgagtag cgaacattgc
     4321 gggcggtccc cgacgcttcg atatcgtctc ggatcatcca gccgtggttg gtcgcagtgc
     4381 cggcgacgaa ggcactgacg tcggctgtga cgtcccatcc gttcacatac cccacggccg
     4441 tgttctggca tccggggccg accgtgatga acgaggtgcg agaaccactc gccggctggt
     4501 tggcggcgtt ccatcccgca ggctgcgtgt tccacgtcaa cccggcttcg gtccaggcga
     4561 aggtcacccg gaagatgtcc tgggtgcgac agacgccggg gatcgcagat gcgtagagac
     4621 ggagggttcc ggagctgatg gtggcgctcg aagggatggc agggctgcat tgcgtcaggt
     4681 cgaatcggat gtaggcccgg cgattcgtcg tggcgcctgc tgtctggacg tccatgaagg
     4741 tggcggtgcc gttgttctga gtgaggttgt tgtgattcac ccaggagtcg gcgaccgtgg
     4801 tcgaccctgc ggcccatgcc gtgagggtgc aggtgcggta gacggcgagc gtcttagccg
     4861 tgagcgtgat cggggcggca gccgcagctg tgggcagcaa cgccagaacg aggacgccga
     4921 tcatcggcca agaagcgaag cgtctccctg actccgacac ctcaggcacc gacgatcgtg
     4981 agatgggtgg tcatcgcatt cgtggccgct atcggagtcg ccggcgtcac cacaaccgag
     5041 cccccaccag cgggaaccac tgccgtggcg ctgacactgg tcgacccgtt gctcaatgtc
     5101 atcgacaacg cgccattgcc acaagcagag gcgatatttg agatggtgac agagacgacg
     5161 ttcgcgcctg aaagggtgcg gacgaccgtt accccgtcgg tgtcacacga cgccaccgcc
     5221 acgttggcgc ctgccaggct ctgtgagctc gacggcaggt tggctgccag cccgggcgca
     5281 atggtggcca cgatcgtcac gaacgcagcc accatcccaa tcaaccagcg tctcatcgcg
     5341 tggttctgtc gatacgtttt aacccgagat gaccaggtgc actccgagaa cgctggcagc
     5401 acttacgttt gccgaactga agtcggcggt gaacgaggtt ccggagcccg gcgtggtgcc
     5461 tgtgacctca cccaacgacg cgttggaggt gccggccagg gtgatcctgt agggcttggt
     5521 gaggcacccg gcagccatac cggtcacggt cacgacacca actcggtagc tggaccctgc
     5581 cacgtacgag gtcgcgtagg tcacggtcag cggagtcgcc tggcaggccg cgacagtcga
     5641 gctgcccgcg ccaagggtct ccgtgttgac agtaaggctg gcggcgaggc ctaggacggc
     5701 accaaatacc gctccggcca ccaccagggc gatcagaatt cttttcatac cacgtcttcc
     5761 taatcagggg aacgtgtctt tatcgccacg aaaatggggg tccttgagta ggtcgctcga
     5821 cctcc
//
LOCUS       DBNHXU010000306         3110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5069541, whole genome shotgun sequence.
ACCESSION   DBNHXU010000306 DBNHXU010000000
VERSION     DBNHXU010000306.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5069541"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..805
                     /gene="ligD"
                     /locus_tag="AC161X_07680"
     CDS             <1..805
                     /gene="ligD"
                     /locus_tag="AC161X_07680"
                     /inference="COORDINATES: protein motif:HMM:TIGR02779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="non-homologous end-joining DNA ligase"
                     /protein_id="HIR4989467.1"
                     /translation="RIHEQVVALDAVLDGELVAFDDGVPSFQTLQQRMHLRNEKQVEQ
                     LSLRIPVVYMVFDLLYMDGRNLTGQPLQERRRILEEAIVASENIQISPSVIGDGVALF
                     RAAAGQGLEGIMAKRLSSLYLPGARSRDWLKVKIKFDADVVIVGWTEGEGQRLGSIGS
                     LMMAVYDGDELRYAGNVGTGFDRDTLQDVLGRLQALSETKPPFPPEVLRSRPELRKAH
                     WVTPSLVAKVEHRQVTSAGRLRSPSFLGLREDKDPKECTYAQLVGEAGP"
     gene            complement(830..1423)
                     /locus_tag="AC161X_07685"
     CDS             complement(830..1423)
                     /locus_tag="AC161X_07685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989468.1"
                     /translation="MFNARLRLSGRSKLPMGVEVDIDHERMTLTAADRTVGVWPLEKL
                     EVSSRADGFHITVDGEEIVLTVTDSKKFAAELGVEQRPRRAAAPKPVPRREVQSPSVD
                     TKSASRANGAVKPPAPVASTKIEPGPSDDVQGRISEIAEALKTDTMSPAEAFAEWLKL
                     MKELNHLHGEGAMPSELFHQLNTQLLDLLPDPVPNPV"
     gene            1593..2399
                     /locus_tag="AC161X_07690"
     CDS             1593..2399
                     /locus_tag="AC161X_07690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989469.1"
                     /translation="MVVVLEDLIEQRPNSVVAIRQRVTNGLLGAHRVWWALFMRSLLV
                     VGFQALFAAAFLIAGESSPWRSAADWWLASFATAEVVNLWLLNRWARAEGIALRDLYN
                     LRRESWTGDVKWLVVALLVAAPLGFLPNILLAQVLWDDPATPADLTFRAVPILAAWVM
                     VGLFPIIHALTELPTYFGYVMPRLEALTGRRWMPLLATSVMLSTQHVFLPLLFDWRYL
                     VWRLLMFLPFALWFGWIVKKRPTVLPYLVVAHALLDLSLPIYVLQASLGG"
     gene            2445..2600
                     /locus_tag="AC161X_07695"
     CDS             2445..2600
                     /locus_tag="AC161X_07695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989470.1"
                     /translation="MPDSPDWTDFNESDPGITNLQLFAFLAIVGIFGVGLISIWQRRR
                     RRRRPIQ"
     gene            2646..2996
                     /locus_tag="AC161X_07700"
     CDS             2646..2996
                     /locus_tag="AC161X_07700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020416283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR4989471.1"
                     /translation="MVVGMQDQEQVDRVFQALADATRRDIVRRAMEGGQSVSNLARQY
                     PMSFAAVQKHVAVLERADLVTKQRHGREQLVHSNLDTIREAFRLLDQLETVWRSRIDR
                     FGEVLAEQGEGETA"
BASE COUNT          553 a          940 c          968 g          649 t
ORIGIN      
        1 gcgcatccac gaacaagtgg ttgctctcga cgccgtgctc gacggcgagc ttgtggcctt
       61 cgacgacggt gtgccctcct ttcagacgct ccagcagcgg atgcacctga gaaatgagaa
      121 gcaggtcgaa cagttgtcac tgaggatccc ggtggtgtac atggttttcg accttctcta
      181 tatggacggc cgaaacctga ccggccagcc actgcaggag cggcgccgga ttctggaaga
      241 ggccatcgtt gcctcggaga acatccagat ctcaccctcg gtcatcggag atggggttgc
      301 cctgtttcgg gccgccgctg gacaaggcct ggaagggatc atggccaaac gcctgtcgtc
      361 cttgtaccta ccgggagctc gatcccgtga ttggctcaaa gtcaagatca agttcgacgc
      421 agacgtcgtg atcgtgggct ggaccgaggg tgagggtcaa cggttgggga gtatcggctc
      481 gttgatgatg gccgtctacg acggcgatga gcttcgctac gccgggaacg tgggcaccgg
      541 attcgaccgg gacaccctcc aagatgtcct cgggcggctg caggctctct ccgagaccaa
      601 accgccgttc ccgcccgagg tcttgcgatc acgtcccgaa ctgcgcaagg cccactgggt
      661 cactccgtcg ctggtggcca aggtggagca tcgccaagtg acgtcggcag ggcggctccg
      721 gtcaccctcg ttcctgggac ttcgtgagga caaggatccc aaggagtgca cctacgctca
      781 gctggtcggc gaggcgggcc cgtagaaaca gggaccggaa tgcgagcgcc tagacggggt
      841 ttggaaccgg gtctggtaac aggtcgagaa gttgggtgtt cagctggtgg aagagctcgg
      901 acggcatggc accctcgccg tgaaggtgat tgagctcctt catgagtttc agccactcgg
      961 cgaaagcctc ggctggcgac atcgtgtccg tcttcagagc ctcggcgatc tcggagatcc
     1021 gaccctgcac gtcgtcggac ggtcccggct cgatcttggt gctggctaca ggtgccggag
     1081 gcttgaccgc tccgttcgct ctcgacgcgc tcttggtgtc aaccgagggc gattggacct
     1141 cccgccgcgg cactggcttg ggcgcagctg cccgccgagg acgctgctcg acaccgagct
     1201 ccgctgcgaa cttcttcgag tcagtgacag tgagcacgat ttcctcaccg tcaactgtga
     1261 tgtggaagcc gtcagcgcgc gaggagacct cgagcttttc caggggccac actcccacag
     1321 ttcggtcggc tgcggtcagg gtcattcgtt cgtggtcgat gtcgacctcc acccccatcg
     1381 gcagcttcga acggcccgat aggcgaagcc ttgcgttgaa caacgacatc ctctctccat
     1441 cctgcgtcgg ccggcaaacg cggtgggtac ccgctccctg tgtaatcgtt gagtaggcca
     1501 aaagccttac ggtcgatgag gaaaagtttc cgagatcgga gtcgacgggt ctttcgtccc
     1561 taccaccgat tccagtgatc gttgatagtt cgatggtggt cgtcctcgaa gacctgattg
     1621 aacaaaggcc gaactcagtt gtcgccattc gacaacgtgt caccaacgga ctgctcggtg
     1681 cccatcgtgt ctggtgggcg ctgttcatgc gcagcttgtt ggtggttggg ttccaagcac
     1741 tgttcgccgc agcctttctg attgctggcg aatcatctcc gtggcgctcc gccgcggact
     1801 ggtggctcgc ctccttcgcc accgccgagg tggtcaatct ctggctgttg aatcgatggg
     1861 cgcgggcgga ggggattgcg cttcgtgatc tctacaacct ccgtcgtgag agttggacgg
     1921 gggatgtcaa gtggcttgtg gttgctctcc tcgttgcggc gccgttgggt ttcctgccca
     1981 acatccttct cgctcaggtg ttgtgggacg atccggcgac accggcggat ctgacgtttc
     2041 gcgcggttcc gatcctggcc gcctgggtga tggtggggct gttcccgatc atccatgcac
     2101 tgaccgagct tcccacctac ttcggctatg tgatgccccg gctcgaggcg ttgaccgggc
     2161 gccgctggat gccactactg gcgacctccg tcatgctgtc cacccagcac gtgttcctac
     2221 cgttgctttt cgattggcga tatctcgtct ggcggttact catgttcctc ccgttcgctc
     2281 tgtggttcgg ttggatcgtg aagaagcgac cgaccgtcct gccctatctc gtggtagcgc
     2341 acgctctcct cgatctcagc ctccccatct atgtgcttca ggcgtctttg ggaggttgac
     2401 tgtcggcttg agtcgacggc taacgcaggt aggttcaggg gccgatgccc gactcacctg
     2461 actggacaga cttcaacgag tccgatccag ggatcaccaa cctccagctg ttcgctttcc
     2521 tcgccatcgt cggcatcttc ggggtggggc tgatttcgat ctggcaacgc cggcggagac
     2581 gtcgccgacc tatccaataa cccggcactt gcgcccggcg catgactgct ctaacctaca
     2641 accaaatggt tgtaggaatg caggatcagg aacaggtcga ccgggtcttc caagccctcg
     2701 cagatgccac gcgaagagac atcgtgcgac gcgccatgga aggcggacag tccgtgtcga
     2761 acctggctcg ccagtatccg atgagcttcg ccgcagtgca gaagcacgtc gccgtgctgg
     2821 agcgggccga cctcgtgacc aagcagcggc acggccgcga gcagctggtc cacagcaacc
     2881 tcgacaccat tcgtgaggca ttccgccttc tcgatcagct ggaaacagtg tggagaagtc
     2941 gcatcgaccg attcggcgag gtcctcgccg agcaaggtga aggagaaaca gcatgaccgt
     3001 gaccaatgtc cacaaggacg cagaggcgat gacgatgacc atcaccgcag agttcgatgc
     3061 gccgatcgaa ccggtgtggc agatgtggga ggaccctcgt cgactcgaaa
//
LOCUS       DBNHXU010000307         2185 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2887595, whole genome shotgun sequence.
ACCESSION   DBNHXU010000307 DBNHXU010000000
VERSION     DBNHXU010000307.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2185)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2185
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2887595"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..179)
                     /locus_tag="AC161X_07705"
     CDS             complement(<1..179)
                     /locus_tag="AC161X_07705"
                     /inference="COORDINATES: protein motif:HMM:NF014588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-C-methyl-D-erythritol 2,4-cyclodiphosphate
                     synthase"
                     /protein_id="HIR4989472.1"
                     /translation="MTAEPSGSRVGWGFDAHRLDGSPPLVLGGVTVSDSIGVSATSDG
                     DVLAHAVIDAVLGACA"
     gene            complement(176..688)
                     /locus_tag="AC161X_07710"
     CDS             complement(176..688)
                     /locus_tag="AC161X_07710"
                     /inference="COORDINATES: protein motif:HMM:NF044704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009303 - rRNA transcription [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CarD family transcriptional regulator"
                     /protein_id="HIR4989473.1"
                     /translation="MAQSASKYAVGDKVVHPQHGAATIQKKVKQEFAGKKQDYFVLDI
                     ATEQLTVMVPLDKVDDLIRPVISKTRSKEVLKSLKGEPEEAGANWSRWYKVLNEKMTS
                     GDIFQVAEVVRDLSFAQQTKGISPALKRMLSKARLTLSSELAFSLEIDEEEAIKRLDR
                     ALPTIEADEE"
     gene            complement(720..1781)
                     /gene="disA"
                     /locus_tag="AC161X_07715"
     CDS             complement(720..1781)
                     /gene="disA"
                     /locus_tag="AC161X_07715"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:NF010009.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA integrity scanning diadenylate cyclase DisA"
                     /protein_id="HIR4989474.1"
                     /translation="MGDPSINETLERLAPGTPMRRALERIIQQGKGGLVVLGNGSGVE
                     AASSGGFTLRRTKFTPARLAELSKMDGGIVLDDEWEIIMACAVHFVPDGTLPTDETGA
                     RHRTAERMAAQTGKPVVAVSEGRRVATLFYNGQKIELATPTEVTAHVNQELQSLDRLR
                     SRLNDSDMRLTRLEVTGLATHRSAVTVLQRAELLDRLGKVVEARAHTLGEDGRIVALQ
                     LNDLMAGVDFTKRAILQDYLRPLRSNSVAKAIAQLRSMTGTELEDPSLVGGELGFPDL
                     DDPAEPRGFRILSQVGRLPDSVRDDIIRHFGSVSKLLNASQAALLGIEGVGETRAGQL
                     RAFFDRLLESAHEWEPVLD"
     gene            complement(1889..>2185)
                     /locus_tag="AC161X_07720"
     CDS             complement(1889..>2185)
                     /locus_tag="AC161X_07720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HIR4989475.1"
                     /translation="LYDKEVFVNVVGGWRIDDPGSDLPVAIAVASSAFDIPLGKLASW
                     GEIGLAGEVRSIPFEARRVEELDRMGLEHRVAPVGGASMRLGEALASAGLASPT"
BASE COUNT          380 a          711 c          692 g          402 t
ORIGIN      
        1 gcgcatgcgc cgaggacggc gtcgatgacg gcatgggcaa ggacatcgcc gtcagaggtt
       61 gccgagaccc cgattgaatc tgaaacagtg actccaccga gcacaagtgg cggggatccg
      121 tcgagacgat gggcgtcgaa gccccaacca acgcgagagc cgctcggctc cgcggtcatt
      181 cctcgtcggc ttcgatcgtt ggtagggccc ggtccaacct cttgatcgcc tcttcctcat
      241 cgatctcgag gctgaaggcc agttccgagc tcagggtgag acgagccttc gagagcatcc
      301 gcttcagcgc cgggctgatc cccttggtct gctgggcgaa ggagagatcc cgcacgactt
      361 cggccacctg gaagatgtcg cccgaggtca tcttctcgtt gagaaccttg taccaacggc
      421 tccagttggc gccggcctct tccggctcgc ccttgagcga cttgagcacc tctttcgacc
      481 tggtctttga gatcaccggc cggatcaggt cgtcgacctt gtccagcggg accatcacgg
      541 tcagctgttc ggtggcgatg tcgaggacga aatagtcctg cttcttccca gcgaattcct
      601 gcttgacctt cttctggatg gtggcggcgc catgctgtgg atggaccacc ttgtccccga
      661 ccgcgtattt cgatgcactt tgagccatga gcgaggacgg atagtaccgg cggccggcat
      721 caatccagca cgggctccca ttcgtgggcc gactcgagca aacggtcgaa gaacgcccga
      781 agctggccgg ccctggtttc gcccactcct tcgatgccga gaagggcagc ctggctggcg
      841 ttgagcagct tcgagaccga gccgaagtgc ctgatgatgt cgtcgcggac cgagtcaggg
      901 agcctcccca cctgggagag aatgcgaaat ccgcggggct cggcagggtc gtccaggtcg
      961 ggaaacccga gttctccgcc gacgaggctc gggtcctcca attcggttcc ggtcatcgac
     1021 cgaagctgag cgatggcctt ggcgacggag ttgctcctca gcggacggag atagtcctga
     1081 aggatcgccc tcttggtgaa gtcgaccccg gccatcaagt cgttgagctg aagggcgacg
     1141 atccggccgt cctcacccag tgtgtgcgcc cgggcttcga ccaccttgcc gagacggtcg
     1201 agcaactcgg cgcgctggag aacggtcacc gccgaacggt gggtggcgag cccggtgact
     1261 tcgagacggg tgagacgcat gtccgagtcg ttgagacggc tccgcaggcg gtcgaggctc
     1321 tgcagttcct ggttgacatg ggcggtgact tctgtggggg ttgccaactc gatcttctgg
     1381 ccgttgtaga agagagtggc gaccctccgg ccctcgctca cggcaaccac cggcttgccg
     1441 gtttgggcgg ccatgcgctc ggcggtgcgg tgccgggcgc cggtctcatc ggtgggcaga
     1501 gtgccgtcgg ggacgaagtg gaccgcgcag gccatgatga tctcccactc gtcgtcgagg
     1561 acgatcccgc catccatctt ggagagctcg gccaggcgag ccggggtgaa cttggtcctt
     1621 cgaagagtga atccgccgga actggccgct tcgactccgg atccattgcc caggacgacc
     1681 aacccgccct ttccctgctg gatgatccgt tccaacgctc ggcgcattgg ggtccctggc
     1741 gccagtcgct ccagggtctc attgatcgat gggtcaccca cgtgcgcagg ctatcagttt
     1801 ggattgccac ccgacccgtg ggaattccgg ccgacgactt ctcgaaattg ggagccacag
     1861 gtccgtatat gacccccgta ggtcccaatt acgtggggga ggccaacccg gcgctggcca
     1921 gcgcctctcc gagccgcatc gatgctcccc ccaccggggc gacccggtgc tccagcccca
     1981 tccgatccaa ctcctcaacc cgacgagcct cgaacgggat cgacctgacc tccccggcca
     2041 acccgatctc cccccacgag gcgagtttcc cgagcggaat gtcgaaggca gaagacgcca
     2101 cggcgatggc caccggaagg tcggacccgg gatcgtcgat ccgccaaccg ccgaccacgt
     2161 tcacgaagac ctccttgtcg tacag
//
LOCUS       DBNHXU010000308         1735 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2293310, whole genome shotgun sequence.
ACCESSION   DBNHXU010000308 DBNHXU010000000
VERSION     DBNHXU010000308.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1735)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1735)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1735
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2293310"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..747
                     /locus_tag="AC161X_07725"
     CDS             <1..747
                     /locus_tag="AC161X_07725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017627091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR4989476.1"
                     /translation="AKNHMIVLPDADIDLAADSAVSAGYGSAGERCMAISAVLAVGEA
                     GDRLIEGVQARIEKLKVGPGDEPGVEMGPLVTRAHLDRVRGYVDAGVAEGATLVADGR
                     SLVVDGHEDGFFLGPTLFDQVTPNMSIYTDEIFGPVLSTVRMEHFEEAIRLINENPYG
                     NGTAIFTNDGGAARKFQQEVQVGMVGINVPIPVPLSFYSFGGWKDSIFGSHAIYGPEG
                     VHFYTRQKVVISRWADPIHRGVDLGFPTHG"
     gene            823..1356
                     /locus_tag="AC161X_07730"
     CDS             823..1356
                     /locus_tag="AC161X_07730"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4989477.1"
                     /translation="MTTAADQRFETLFRSHHQMVLSYCRRRMAPSDAEDVAADVFAVA
                     WRRMDDVPEGEAAKAWLLAVAYKTIGNHRRGSSRRSFLGGKLAAQRESEQETPEARVT
                     VRDEDLHLLASLQKLRPSDQEILRLVAWDKTSHAQIGIVLGISEDAVSQRISRARARL
                     KKEYNKLQSPEGAVSRD"
     gene            1349..>1735
                     /locus_tag="AC161X_07735"
     CDS             1349..>1735
                     /locus_tag="AC161X_07735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989478.1"
                     /translation="MTDLLDRLAALDPVSTGITEQDATRLRATVADRIDGGAVKLRPT
                     PQRPGGRRLTLVGVGVFALVIVIFVPVYLAQRPETMVDAATEDTLRDLPGVQDVIRVG
                     TVGGVRTSAIDGDTMWVVSSNDHELYR"
BASE COUNT          321 a          547 c          556 g          311 t
ORIGIN      
        1 gccaagaacc acatgatcgt gttgccggac gccgacatcg atctcgctgc agactcggcg
       61 gtgtctgccg gctacggctc ggccggcgag cgctgtatgg cgatctcagc cgtgctcgcc
      121 gtgggcgagg cgggtgaccg tctcatcgag ggggttcagg ctcgtatcga gaagctgaag
      181 gtcggtcccg gtgacgagcc cggggtggag atgggccccc tcgtcaccag ggcacatctc
      241 gaccgggtgc gcggctatgt cgacgccggg gtggccgaag gggcgacgct cgtggccgac
      301 ggccgaagcc tggttgtcga cggccatgag gacggcttct tcctcggccc gaccctcttc
      361 gatcaggtga cgccaaacat gtccatctac accgacgaga tcttcggccc ggtgctgtcc
      421 acggttcgca tggagcactt cgaggaggcg atccgcctca tcaacgagaa tccatatgga
      481 aacggaaccg cgatcttcac caacgacgga ggggccgccc gcaagttcca acaggaggtc
      541 caggtgggga tggtcgggat caatgtgccg atccccgtcc cgctctcctt ctacagcttc
      601 ggcggttgga aggactcgat attcggcagc cacgccatct atggccccga aggagtccat
      661 ttctacaccc gccagaaggt ggtcatctcg cggtgggcgg atccgattca tcgcggagtg
      721 gacctggggt tccccacaca cggctgacac ctgtcagctt ccggctcgaa cggacactta
      781 gcccatgaag agggtgaatt gtccagaacc gagctactcc aagtgaccac cgccgctgac
      841 cagcgattcg agaccttgtt ccggtcgcat catcagatgg tcctctctta ctgccgtcgg
      901 cgaatggcac cctccgatgc cgaagacgtc gcagctgacg tatttgccgt cgcctggcgg
      961 cgaatggacg acgttccgga gggagaagcg gcgaaggcct ggctcctggc cgtggcctac
     1021 aagaccatcg gcaaccatcg tcgcgggagc agccgacgct cgtttctcgg aggaaagctt
     1081 gcagcgcaaa gagaatccga gcaggagacc ccggaggccc gggtcaccgt tagagatgag
     1141 gacctccacc ttctggcgtc gctgcagaag ctgcgcccct ccgatcaaga gattttgagg
     1201 ctggtggcct gggacaagac gagtcatgcc cagatcggca tcgtgctcgg catcagcgag
     1261 gacgcggtgt cccaacgcat ttcgcgcgcc cgggctcgtc tcaaaaagga atacaacaaa
     1321 ctgcaaagcc ccgaaggagc tgtttcccgt gactgaccta ctcgatcgtc tggccgccct
     1381 cgacccggtg tcgacgggta tcaccgaaca agacgccacc agacttcgcg ccacggtcgc
     1441 tgatcggatc gacggcgggg cggtcaagct tcgccctact ccgcagcgcc ccggtggtcg
     1501 tcgcctcacc ctggtcgggg tgggcgtctt cgccctggtc atcgtgatct tcgttccggt
     1561 gtatctcgct cagaggccag agaccatggt ggatgccgcc accgaggaca cactgcgtga
     1621 cctgcccggg gttcaggacg tgatccgggt cggcaccgtg ggtggggtgc gtacctccgc
     1681 aatcgacggg gacacgatgt gggtggtcag ctccaacgat cacgagctgt atcgg
//
LOCUS       DBNHXU010000309         3812 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4875219, whole genome shotgun sequence.
ACCESSION   DBNHXU010000309 DBNHXU010000000
VERSION     DBNHXU010000309.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4875219"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..632)
                     /locus_tag="AC161X_07740"
     CDS             complement(<1..632)
                     /locus_tag="AC161X_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018683195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989479.1"
                     /translation="MSVSAPSRLTPSGRMTPFRAQRIWERNVFVYRRLWKIIFTGFFE
                     PVFYLFSIGLGIGALVGDVPGPNGIPIPYTAFVAPALMAASAMNGAVLETTFNIFFRL
                     KFENVYEGMLTTPLVPRDIAIGEIGWALLRGAAYSTVFLVVMALMRLTESWWALLTVP
                     GAILIGFCFGSLGMAATTYMRSWQDLDLIALVTMPLFLFSATFYPLDVYPP"
     gene            complement(629..1417)
                     /locus_tag="AC161X_07745"
     CDS             complement(629..1417)
                     /locus_tag="AC161X_07745"
                     /inference="COORDINATES: protein motif:HMM:NF013245.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989480.1"
                     /translation="MTALTRSFRVVEAELVVYRRTWRGTVISSFVNPLLFLSAMGLSL
                     GTLIDSSGADLGVPYLTFVASGLMAATAMQAGAGEGSFPVMAGIKWRKEFYATIATPL
                     APADIVYGRFLWTIIRLTFILVVFAIISVLFGALELGPALLAVAPAVLTGVAFTTTVT
                     AFTVTQENDTSLSTLFRFGITPLFLFSGTFFPISQLPAFLQKVAYVTPLFHGVELVRK
                     VALPEADQSVVTAMPMWIHVVYLTAMTVIGLVLSARLLDKRLRP"
     gene            complement(1417..2343)
                     /locus_tag="AC161X_07750"
     CDS             complement(1417..2343)
                     /locus_tag="AC161X_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015098851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989481.1"
                     /translation="MPESIVQARGLTKKFGDFVAVDSIDFDIVKGEAFGFLGPNGAGK
                     TSTMKMITTVSPITDGKLTVLGLDPTTQGRSIRQRIGVVPQEDNLDLELTVAENLYVY
                     GRYHDMNRADIRRRTEELLEFAQLTDRAKSQVEPLSGGMKRRLTIARALINDPELVIL
                     DEPTTGLDPQARHVLWDRLYRLKQQGATLIITTHYMDEAEQLCDRLVVMDKGVIAAEG
                     SPRELISKYAPREVVELRFLDGEQDQAVLKLDGLAHSSEVLADRVLLYTDDAEATQHE
                     MGERGVHPEQVLVRRASLEDVFLKLTGRRLID"
     gene            complement(2405..2899)
                     /locus_tag="AC161X_07755"
     CDS             complement(2405..2899)
                     /locus_tag="AC161X_07755"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR4989482.1"
                     /translation="MPDIYDFILKLRAIRDFKREPLASEDLAAVLEAARWTGSSKNRQ
                     DWSFVAVTDRDRLQGLAEHGDFTQPVRDSAATIVLVQEPGGNMFDVGRVAQNIMLASK
                     AIGVASCPITLHRDAQARHFLGAPDDRVTRYAVALGYAAEGAAPKNYGGRKPSESVVH
                     LDRY"
     gene            2932..>3812
                     /locus_tag="AC161X_07760"
     CDS             2932..>3812
                     /locus_tag="AC161X_07760"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR4989483.1"
                     /translation="MRGSVTLAGDRWRVGSRRFADPEQVLSAESLDEVLPVMAQAEAA
                     AQSGSWVVGYVSYQAAPAFDSPLRVTSRRQGPELPLAWFGVYPASAPPPPAVGSFRLG
                     SWSAGLSETDHATRVETIREAIAAGDTYQVNLTFPMWTDFAGDPGALFQAMERSQPHS
                     YGAWLDIGDHHIVSVSPELFFALDELLITTRPMKGTAPRGRSSAEDLEHRKALLHSEK
                     ERAGNLMIVDMLRNDLGRVAEIGSVEVPVLFDVERHPTLWQMTSTITARMPADLGLGG
                     LFTGIFPSASVTGAPKVS"
BASE COUNT          740 a         1154 c         1245 g          673 t
ORIGIN      
        1 ggcgggtaga cgtccaaggg gtagaaggtg gccgagaaca ggaacagagg catcgtgacc
       61 agggcgatca gatcaagatc ctgccaagag cgcatgtagg tcgtggctgc catccccaga
      121 gatccgaagc agaatccgat caggatcgcc cccggcaccg tgagcagggc ccaccacgac
      181 tccgtgaggc gcatgagggc catgacaacc aagaacactg tcgaataggc ggcacccctg
      241 aggagtgccc acccaatctc cccgatcgcg atatccctgg gaaccagcgg ggtggtgagc
      301 atcccttcgt agacattttc gaatttgaga cggaagaaga tgttgaaggt cgtctcgagt
      361 accgctccgt tcatcgccga ggcggccatc agagcgggtg cgacaaatgc cgtgtatggg
      421 atgggaatgc cgttggggcc agggacgtcg ccgaccagcg ctccgatccc gagaccgatg
      481 gagaagagat agaacaccgg ctcgaagaat ccggtgaaaa tgatcttcca cagcctgcgg
      541 tacacgaaga cgtttcgctc ccagattcgc tgggcgcgga aaggcgtcat ccgacccgac
      601 ggggtgagcc gagatggggc cgagacgctc acggcctgag ccttttgtcg aggagcctgg
      661 cggagagcac cagcccgatc accgtcattg cggtcaggta gacgacatgg atccacatcg
      721 gcatcgcggt taccaccgac tgatcagctt cgggcagcgc caccttgcgc accaactcca
      781 ccccgtgaaa caacggggtg acataggcga ccttctggag aaaggcaggt agctggctga
      841 tcggaaagaa ggtgcctgag aacaggaaga gcggagtgat cccgaaccgg aacaatgtgg
      901 agagactggt gtcgttttcc tgggtcaccg tgaaggcggt cacggtcgtg gtgaaggcga
      961 caccggtgag cacggccggt gccaccgcca acagggccgg gccgagctcg agggcgccga
     1021 acaggacgga gatgatggcg aagaccacga ggatgaaggt gagtcggatg atggtccaga
     1081 ggaagcgccc atagacgatg tcggccgggg cgagcggagt ggcgatggtg gcatagaact
     1141 ccttgcgcca cttgatcccc gccataaccg ggaacgaccc ctccccggca ccagcttgca
     1201 tcgctgtcgc cgccatcaac ccactggcca cgaaagtcag gtaggggaca cccaggtcgg
     1261 cgccgctgga atcgatgaga gtgcccaggc tgagtcccat cgccgacagg aacagcaagg
     1321 ggttcacgaa actggagatc accgtgcccc gccaggtgcg gcggtacacc accaattcgg
     1381 cctcaaccac tcggaaggaa cgggtgaggg cggtcatcag tcgatcagtc tccgcccggt
     1441 gagcttgagg aacacatcct cgaggctggc gcgacggacc aacacttgct cgggatggac
     1501 gcctcgctca cccatctcgt gttgggtggc ctcggcgtcg tcggtatata gaaggacccg
     1561 atcggccagc acttcggaac tgtgagccag gccgtcgagc ttcaagaccg cctgatcctg
     1621 ctcgccgtcc aggaaacgaa gctcgaccac ttcgcgtggc gcgtacttcg agatcaactc
     1681 ccgcggtgag ccttcggccg cgatgacacc tttgtccatg accaccaatc gatcacaaag
     1741 ttgttccgcc tcatccatgt agtgagtggt gatgatcagg gtcgctccct gctgcttcaa
     1801 gcgatacagc ctgtcccaga gaacgtggcg cgcctgggga tcgagtccgg tggtgggctc
     1861 gtcgagaatc accagctccg ggtcgttgat gagagcccgg gcaatggtga gacggcgttt
     1921 catcccgccg gaaagcggct ccacttgcga cttggcccga tcggtgagct gggcgaactc
     1981 cagcagctct tccgtgcggc gccggatgtc ggcgcggttc atgtcgtgat accggccgta
     2041 gacatagaga ttctcggcca cggtgagctc cagatcgaga ttgtcctcct gggggacgac
     2101 gccgatccgt tgccggatcg aacggccttg agtggtggga tcgagcccga gaaccgtgag
     2161 cttcccgtcg gtgattgggc tcaccgtggt gatcatcttc atggttgagg tcttcccggc
     2221 tccgttgggg ccaaggaatc cgaaggcctc acccttcacg atgtcgaagt cgatcgagtc
     2281 gaccgcgacg aaatcgccga acttcttggt gaggccgcgc gcctggacta tcgactcggg
     2341 catacgtgtc cggacggtag tgccgatggg ggacagaact tcccggtatt aggagcgatg
     2401 tcgatcagta gcggtcgagg tggacgaccg actcggaggg cttacgcccg ccgtagttct
     2461 tcggggccgc tccttctgcg gcgtagccaa gcgccacggc atatcgggtc acccggtcgt
     2521 cgggcgcgcc caggaagtgg cgggcctggg catctcggtg gagcgtgatg gggcaagagg
     2581 ccaccccgat cgccttggag gccagcatga tgttctgggc cacacggcca acgtcgaaca
     2641 tgttccctcc cggctcttga accaaaacga tggtggcggc agagtcccgc accggttgcg
     2701 tgaaatcacc gtgctcggct aggccctgaa ggcggtcgcg gtcggtgaca gcgacgaagg
     2761 accaatcctg gcggttcttc gagcttccgg tccatcgtgc ggcctcgagg actgcggcga
     2821 ggtcctcgct tgccagcggc tcccgcttga agtcacggat ggcacgcagc ttgaggatga
     2881 agtcgtagat gtcgggcatg gcccatcgac cctaccggta acgtcgctcg ggtgagaggc
     2941 tcggtgactc tggctggtga ccgttggagg gttggctcac gccggtttgc cgatccggag
     3001 caggtcctgt cggcggagag cctggacgag gtgttgccgg tcatggcgca agccgaggca
     3061 gccgctcaga gcgggtcttg ggtagtcggc tacgtctcct atcaagctgc tccggccttc
     3121 gattcgccgc tgcgggtgac ctcacgacgc caggggcctg agcttcccct cgcctggttt
     3181 ggcgtgtacc cggcatcagc accaccgccg ccggcggtcg gctcctttcg cctcggaagt
     3241 tggagcgcag ggctctccga gaccgatcac gcgacacggg tggagacaat ccgcgaggcg
     3301 atcgccgcgg gagacaccta tcaggtcaac ctaacctttc ccatgtggac cgacttcgct
     3361 ggcgatccag gtgccttgtt tcaggcgatg gagcgaagtc agccgcacag ctacggggcg
     3421 tggctcgaca tcggtgacca ccacatcgtc tcggtctccc cggagctgtt cttcgccctc
     3481 gacgagctgc tgatcacgac ccgacccatg aagggcaccg cgccccgcgg ccggtcttcg
     3541 gccgaggacc tcgagcatcg aaaagccttg ctccactccg agaaggaacg agccggcaat
     3601 ctgatgatcg tcgacatgct tcgcaacgat ctgggccgcg tagccgagat cggatcagtc
     3661 gaggtaccgg tacttttcga cgtggaacgt catcccaccc tttggcagat gacgtcgacc
     3721 atcaccgccc gcatgcccgc cgatctcggc ctgggtgggc tcttcacagg gatctttccc
     3781 tcggcttcgg tgactggcgc tccgaaggtg tc
//
LOCUS       DBNHXU010000310         2547 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1105336, whole genome shotgun sequence.
ACCESSION   DBNHXU010000310 DBNHXU010000000
VERSION     DBNHXU010000310.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2547)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2547
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1105336"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..530)
                     /locus_tag="AC161X_07765"
     CDS             complement(<1..530)
                     /locus_tag="AC161X_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HIR4989484.1"
                     /translation="MSASGGTRAIIAALLANAGIALAKFIAYVVTGSASMLAESIHSV
                     ADTANQGLLLLGGRQSRRVADQEHQFGYGRERYFWSFVVALVLFSLGGLFSIYEGISK
                     ILHPHELTSIGWAIAVLLVAIVLEGFSFRTAIVESRKVKGERKWVEFVRTSRSPELPV
                     VLLEDAGALCGLFLALV"
     gene            complement(540..758)
                     /locus_tag="AC161X_07770"
     CDS             complement(540..758)
                     /locus_tag="AC161X_07770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989485.1"
                     /translation="MADLFDIQNLLPELVLGLGLALLFGNGLAWWKHRQGETPQGVVD
                     AQFRRGRVIFLLVVGLLMTTWGAVTLLT"
     gene            793..1659
                     /gene="thrB"
                     /locus_tag="AC161X_07775"
     CDS             793..1659
                     /gene="thrB"
                     /locus_tag="AC161X_07775"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009088 - L-threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HIR4989486.1"
                     /translation="MIQDQTAAFASAPATSANLGPAFDCLGLALDMRCQVTARLAESW
                     SVKHVGRFRPAAGEGDGVLGAARKVVGDDQPLALEVDANIPMGKGLGSSAAAFVAGAA
                     AAMRALGDEANPDKVFRVAAELEGHPDNVAAAVYGGLVLVPAEGMPIRLPIHPTLRVV
                     VAIPEGNLPTDDARRVIDSVHDHDVVRRSLARVAALTAGLITGDPETLAAAHGDEIHE
                     APRAELSPEVNGLIEIAKRAGALHAARSGAGPSVLALCSVDQAVRVAGAFRESGLEVL
                     EATIATSGLVCG"
     gene            complement(1667..1822)
                     /locus_tag="AC161X_07780"
     CDS             complement(1667..1822)
                     /locus_tag="AC161X_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989487.1"
                     /translation="METEKEKADRDERERKEEGDEYERARKEENERNERERKEESERY
                     ERERKGT"
     gene            1928..2467
                     /locus_tag="AC161X_07785"
     CDS             1928..2467
                     /locus_tag="AC161X_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019073337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4989488.1"
                     /translation="MNSDIERRQRFELIAAQVYEPLQRYLRRRATPEDAGDALGDVLL
                     TVWRRLDDVPADLPLPWCYGVARRILANQRRGAQRHLRLVDRMNSEAAISRSHDVDDP
                     ELEAALAGLSEDDQELLRLWAWEQLEPREIAVVLMTTPNAVSLRLTRAKAKLEKKLTR
                     QDPSISGQKPDRHTEEHRP"
BASE COUNT          456 a          826 c          801 g          464 t
ORIGIN      
        1 accagagcca ggaagaggcc acacaaggcc ccggcatcct cgaggagaac caccggcaac
       61 tccggtgagc gcgaggtccg cacgaactcc acccacttgc gttcgccttt gacctttcgt
      121 gactccacga tggcggtgcg gaagctgaaa ccctccagaa cgattgccac gagtaacacg
      181 gcgatcgccc agccgataga cgtcaactcg tgggggtgaa ggatttttga gatcccttcg
      241 taaatcgaga agagcccgcc aagcgagaac aggaccagag ccaccacgaa cgaccagaaa
      301 tagcgctctc ttccgtatcc gaattggtgt tcctggtcgg cgactctccg agattggcgg
      361 ccgcccaaca ggagcagacc ttggttggcg gtgtcggcca ccgagtggat cgactcggcc
      421 agcatcgatg ccgatccggt cacgacatac gcgatgaact tggcaagagc gatgccggcg
      481 ttggccaaca gtgcggcgat gatcgccctg gttcctccgc ttgcggacat ggcccgatgc
      541 taggtgagga gggtgaccgc cccccaggtg gtcatcaaca ggccgacgac cagcaggaat
      601 atcactcttc cccgtcgaaa ctgggcgtcg accactccct ggggggtctc cccctggcga
      661 tgtttccacc aggcgagacc gttgccaaag aggagggcga ggccgagacc gaggaccaat
      721 tctggaagca ggttctggat gtcgaagagg tcggccactt cgggtcacgg taacgctgct
      781 taatctccgc tcgtgattca ggatcagacc gccgcatttg cctcggcgcc cgccacgtcg
      841 gccaacctgg gcccggcctt cgactgtctc ggcctggcac tggacatgag atgtcaagtg
      901 acggcccgcc tcgccgagag ttggagtgtg aagcacgtcg ggcgtttccg ccccgccgca
      961 ggggagggcg acggggtgct cggtgccgcc cgaaaggtgg ttggcgatga tcagccactc
     1021 gcgctggaag tcgacgccaa catccccatg ggcaaagggt tgggctcctc cgccgccgcc
     1081 ttcgtggcgg gagccgcggc ggcaatgcgg gcgctaggtg acgaggccaa ccccgacaag
     1141 gtgttccggg tcgcggccga gttggaaggc catcccgaca acgttgccgc cgcggtttat
     1201 ggcgggttgg tcctggtgcc cgcagagggg atgccgatcc gcctcccgat tcatcccact
     1261 ttgcgtgtcg tcgtggccat tcccgagggg aatcttccca ccgacgatgc tcgccgggtc
     1321 atcgattcgg tccatgacca cgacgtggtg cggcggagcc tggcccgggt cgccgcgctt
     1381 acagcggggc tgatcaccgg agatccggag acactcgccg cggctcatgg cgacgaaata
     1441 cacgaggcgc caagggccga attgtcgccg gaggtgaatg gtttgatcga gatcgccaaa
     1501 cgggcaggtg cccttcatgc ggcacgttcc ggagccgggc cgtcggttct ggccttatgt
     1561 tcggtagatc aggcggtaag ggttgcagga gcctttcggg agtcgggtct cgaagtgttg
     1621 gaggccacca tcgccaccag tggcctggtt tgcggctgag ccgctctcag gttcccttgc
     1681 gctcacgctc gtaacgctcg ctctcttcct tgcgctcccg ttcgttgcgc tcgttctctt
     1741 ccttgcgggc ccgctcgtac tcgtccccct cctccttgcg ctcacgctcg tctcggtcgg
     1801 ccttttcctt ttcggtttcc atcgccatct ccagaccttt gactctctga cctccgaggc
     1861 tacaaccggc gtgacgcttc ggccccaggc ctgacagaat caggtctgtg ccggacagaa
     1921 tctctcagtg aactccgaca tcgagcggcg gcaacgcttc gagctcatcg ccgcccaggt
     1981 gtacgagccg ctccagcgct acctgcgacg ccgcgccacg cctgaggacg cgggcgatgc
     2041 cctcggcgac gtcctgctca ccgtgtggcg acgcctcgat gacgtccccg cggacctgcc
     2101 tttgccctgg tgttacgggg tggcacggcg aatactggcc aaccaacgac gtggggccca
     2161 gcgtcatttg cgactggtgg acaggatgaa tagcgaggct gcgatctcgc gttctcatga
     2221 cgtcgacgat cccgagctcg aggccgccct cgccgggttg tccgaggacg atcaggaatt
     2281 gcttcgcctc tgggcgtggg agcagttgga gccccgagag atcgcagtcg tgttgatgac
     2341 cacacccaac gcggtcagcc tccgcctcac cagggccaag gccaaattgg aaaagaagct
     2401 gactcgacag gatccctcca tctccggaca gaaacctgac cgacacaccg aggagcatcg
     2461 accgtgaccg acgaattgcg caatcgcctg agccggctcg acccgatggc ttccgatgtg
     2521 ccgacggagc cagtgacgac cgaatcg
//
LOCUS       DBNHXU010000311         2043 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1502969, whole genome shotgun sequence.
ACCESSION   DBNHXU010000311 DBNHXU010000000
VERSION     DBNHXU010000311.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2043)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2043)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2043
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1502969"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /gene="guaA"
                     /locus_tag="AC161X_07790"
     CDS             complement(<1..914)
                     /gene="guaA"
                     /locus_tag="AC161X_07790"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020463431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HIR4989489.1"
                     /translation="MSPETGQEPTVLVLDFGAQYAQLIARRVRESHVYSEVVSRDITA
                     AEIRARRPIGLILSGGPASVYAENAYRMDPSILDLGIPVLGICYGHQLLAEMTGGTVA
                     NTGSGEYGNTNLEVTGDSLLLQGLPAEQPVWMSHQDQVTTAPEGFVEVARTVGASVAA
                     MEDRSRGLYGVQFHPEVAHTPRGDEILKRFLYDVCHASPTWTSHSIIETQIAAIKAQV
                     GGERVVCGLSGGVDSAVAAALVHAAVGDQLTCIFVDHGLLRMGEGEQVELTFRSHFAV
                     DLIHVKAEDRFLEALAGVTDPEEKRRIIG"
     gene            complement(911..>2043)
                     /locus_tag="AC161X_07795"
     CDS             complement(911..>2043)
                     /locus_tag="AC161X_07795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015797926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GuaB3 family IMP dehydrogenase-related protein"
                     /protein_id="HIR4989490.1"
                     /translation="SGRQAYGFDDIAIVPSRRTRDPDDVDISWQLDAFKFPLPMMGSA
                     MDSAISPATAITIGKLGGLAVLNLEGLWTRYEDPQPYFDEIAELSKEKATLRMQEIYL
                     EPVNPDLIGQRVGEVKAAGVVTAASLTPQRVRSWAPGALKAGLDILVIQGTVVSAEHV
                     SSREEPLNLKEYIANCDAPVIVGGCASYSTALHLMRTGAVGVLVGVGPGAACTTRGVL
                     GVGVPQATAIADAAGARIRYLDETGRYVHVIADGGMRTGGDVAKAIACGADAVMIGSP
                     LASATEAPGRGYHWGMATFHPTLPRGARVEVGDRGTLTEILVGPAHENDGRLNLFGAL
                     TVSMATTGYSDVKEFQKAEVMIAPSIQTEGKSLQRNQRVGMG"
BASE COUNT          388 a          663 c          634 g          358 t
ORIGIN      
        1 ccgatgatcc ttcgcttttc ttcgggatcg gtgaccccgg ccagagcctc gaggaaccgg
       61 tcttcggcct tgacgtgtat caggtcgact gcgaaatgtg agcgaaaggt gagctccacc
      121 tgctcgcctt cgcccatacg gagcaggccg tggtcgacga agatgcaggt gagctgatca
      181 cctaccgcag cgtgaaccaa tgccgcggca acggcagaat cgacgcctcc ggagaggccg
      241 cagacgaccc tctctccacc gacctgggcc ttgatggccg cgatctgggt ttcgatgatc
      301 gagtgtgaag tccacgtggg agacgcatgg cacacgtcgt agaggaaccg cttgaggatc
      361 tcgtcgccgc gcggggtgtg ggcaacctca gggtgaaact ggacgccata gaggccgcga
      421 ctccgatcct ccatcgcggc aaccgaggcc cccacggttc gggcaacttc cacgaaaccc
      481 tcgggcgccg tcgtcacttg atcctggtgt gacatccaga ccggctgctc ggcgggaagg
      541 ccctgaagca gcagcgaatc cccggtcacc tcgagattgg tgtttccgta ctcaccactt
      601 ccggtgttgg ccaccgtgcc accggtcatc tcggcaagaa gctggtggcc gtaacagatc
      661 ccgaggaccg gtatccccag atcgaggatc gaaggatcca tgcgataggc gttctcggcg
      721 taaacggacg ccggaccacc ggagaggatc agcccgatcg ggcgtctcgc ccgaatctca
      781 gcggcagtga tatcccggga gaccacctcg gaatagacgt gcgactcgcg gactcgacgg
      841 gcgatcagtt gggcatattg agcgccaaag tcgaggacaa gaaccgtagg ttcttgtccg
      901 gtctccgggc tcatcccatc cctacccgct ggttgcgttg cagggatttg ccctcggtct
      961 ggatcgaggg cgcgatcatc acctcggcct tttggaactc cttgacgtcc gagtatccgg
     1021 tggtcgccat cgacacggtc agagcgccga acaggttgag ccggccatcg ttctcatggg
     1081 cgggaccgac caggatctcg gtgagggtgc ctcggtcacc gacttcgacc cgggcgccac
     1141 ggggcaacgt cgggtggaag gtggccatcc cccagtgata accccgacct ggggcttcag
     1201 tcgcggatgc gagcggagag ccgatcatca ccgcgtctgc gccacaggca atcgccttgg
     1261 ccacgtctcc cccggtccgc atcccaccgt cggcgatcac atggacgtac cggccggtct
     1321 cgtcgagata gcggatcctg gcaccggcag catcagcgat ggcagtcgcc tgaggcaccc
     1381 caacaccgag gacacctcgc gtggtgcatg ccgcacccgg accgacacca accaagacac
     1441 caaccgcccc agtgcgcatc aggtgcagcg cagtcgagta gctggcgcaa ccaccgacga
     1501 tcaccggtgc gtcgcaattg gcgatgtact ccttgaggtt gagcggctcc tcacgactcg
     1561 agacatgctc cgctgagacc accgtcccct gaatcaccag gatgtcgaga cccgccttga
     1621 gcgcccctgg agcccaagag cgaacccgtt gcggagtgag gctcgctgcg gtgacgaccc
     1681 cggcggcctt cacctcgccc acacgctgac cgatcagatc ggggttgact ggctcgaggt
     1741 agatctcctg catgcgaagg gtggccttct ccttggagag ctcggcgatc tcgtcaaagt
     1801 acggctgtgg gtcctcgtaa cgggtccaga gcccctccag attgaggact gcaagacctc
     1861 ccagtttgcc gatggtgatc gcagtcgcag ggctgatcgc gctgtccatc gccgagccca
     1921 tcatcggaag cgggaacttg aaagcgtcga gctgccagga gatgtccacg tcgtcgggat
     1981 cgcgggtccg cctcgaaggg acgatcgcta tgtcgtcgaa accgtatgcc tgacgccctg
     2041 act
//
LOCUS       DBNHXU010000312         2706 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3884375, whole genome shotgun sequence.
ACCESSION   DBNHXU010000312 DBNHXU010000000
VERSION     DBNHXU010000312.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2706)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2706)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2706
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3884375"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1685)
                     /locus_tag="AC161X_07800"
     CDS             complement(<1..1685)
                     /locus_tag="AC161X_07800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989491.1"
                     /translation="MTVEIGRKAVERREWGDAVAAFHDADHADPLSPEDLELLADAAW
                     WAGEPDESVEVLERAHTGYIEQGSDTGAARVAVALGYLAARRQAFSIAGGWLARAHRL
                     LEGHPESVVNAQIKVIEIIETLYVRGDVAGAIATADETVTLARRLGSRDAESQALAFK
                     GIALIMVGQWKEGLGLLDEATAAAVSGEIGLRAASDIYCQTISACRAMADFRRAGEWT
                     EEADRWMNRYSVTGYTGVCSVHRAELKRLHGNWPEAQEEARAACVDLERYHIIDAVGY
                     AYHEIGEVLFRTGDLVGAEKAFLMAYENGENPQPGLALLMLARGEGAAAASSLASALA
                     QTDKGILLARVTMLPAQVEIALTQNDIETARVAIEELESITAEFERPAFEAATLTAKG
                     DLALHLGEAEEAAVHLERAWRLWKEVEFPYETARARVLLGQARANLGDAGSARMELGA
                     ARSTFERLGATLDLKRVDELLYESRAPDADRDRVTKTLMFTDIVVSTDLVRTLGEDRW
                     ENLLRWHDNELRSLFGRHQGVEVSHTGDGFFVTFDRPSDAVEAAVAIQRRMTE"
     gene            complement(1765..2256)
                     /locus_tag="AC161X_07805"
     CDS             complement(1765..2256)
                     /locus_tag="AC161X_07805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989492.1"
                     /translation="MNVYVIVLRVLHVGAGIFWAGSILILLRFLIPTAKAVGPAAGPF
                     MGRLTGEAKLGEIAGAAGVITILSGILLYWHDFGEVIPFNAAMAGFAIGGVAAIALWI
                     VGMFQLRPANEKLAALGARAAQGEDVGAEMGQTMAARDRLIPVMTILVVVAILAMAIS
                     RYL"
     gene            2334..>2706
                     /locus_tag="AC161X_07810"
     CDS             2334..>2706
                     /locus_tag="AC161X_07810"
                     /inference="COORDINATES: protein motif:HMM:NF012512.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HIR4989493.1"
                     /translation="MTNPWRSTGLPGSSGDEVSVNPSKAIDLLARCPASGVTPLLDLP
                     EVARDLEVDHVWIKDERNRMGLGSFKALGAAYAIAHDADVRLGGDWDQSPHDLANILA
                     DVTYTAASAGNHGLSVAAGARV"
BASE COUNT          500 a          899 c          813 g          494 t
ORIGIN      
        1 tgctcggtca tccggcgctg aatggcgacc gccgcttcga ccgcgtccga aggacggtcg
       61 aaggtcacga agaagccgtc gcctgtgtgg ctgacctcga caccctggtg cctgccaaac
      121 agcgagcgaa gctcgttgtc atgccagcga aggaggttct cccatcgatc ctcgcccagg
      181 gtccggacga ggtcagtcga caccacgatg tcggtgaaca tcagcgtctt tgtcacgcgg
      241 tcgcgatccg cgtccggcgc ccgcgattcg tagaggagct cgtcgactcg cttcaagtcg
      301 agggtcgccc ccagtctctc gaaggtggag cgggcggcac ccaactccat ccgagcggaa
      361 ccggcatcgc cgaggttggc tctcgcctgg cccagcagca cccgtgctcg ggcagtttca
      421 tatggaaact cgacttcctt ccacaacctc caggcccgct ccaggtgaac cgccgcctct
      481 tcggcctcgc ccaagtgcaa agccagatca cctttggcgg tgagagtggc cgcttcgaaa
      541 gcgggccttt cgaactcggc ggtgatcgac tcgagctcct cgatagcgac tcgagcagtt
      601 tcgatatcgt tttgggtcag tgcgatctcg acctgagcgg gcagcatcgt gacccgggcc
      661 aacaggatgc ccttgtccgt ttgagcgaga gcgctggcga gggaggaggc ggcggccgcg
      721 ccttcacctc tggccagcat cagcagcgct agacctggct gtgggttctc accgttttca
      781 taggccatca gaaatgcctt ctcagctccg acgaggtccc cggtacggaa caggacctcg
      841 ccgatttcgt ggtaggcgta gccgaccgca tcgatgatgt gataacgctc aagatcgacg
      901 caggcggcgc gggcctcctc ctgggcttcg ggccagttcc cgtgcaacct cttcaattcc
      961 gcgcggtgga ccgagcagac accggtgtag ccggtgacgg agtatcggtt catccaccgg
     1021 tcagcctcct cggtccattc gccggcccgg cgaaaatccg ccatggcccg gcaggccgag
     1081 attgtttggc aatagatgtc gctcgccgct ctcaggccga tctcgccgga caccgcggcc
     1141 gcagtcgcct cgtcgagcaa acccaagcct tccttccatt gccccaccat gatcagtgcg
     1201 atacctttga acgccagggc ctgggactcg gcgtcacggc tccccaaacg acgggcgagc
     1261 gtcaccgtct cgtcggcggt tgcgattgcc ccagccacat cgcccctgac gtaaagggtc
     1321 tcgatgattt cgatgacctt gatctgggca ttcaccaccg attcgggatg cccttcgagg
     1381 agtcgatgcg cccgtgccag ccaccctcca gcgatcgaga aggcttgacg acgcgcggca
     1441 aggtatccga gggccacggc gacccgggct gctccggtgt ccgatccctg ttcgatataa
     1501 ccggtgtggg ccctctcgag cacctccaca gactcatcgg gctcacccgc ccaccaggcg
     1561 gcatcagcga gcaattcgag gtcctcgggc gagagaggat cagcatggtc tgcgtcgtgg
     1621 aaggcagcga ccgcatcacc ccactcgcgg cgttcgacgg ccttgcggcc gatctcaacg
     1681 gtcataacca agaactcacc tcagcacacg attccaactg attctacgta ccgaatgttg
     1741 gattggttca ggcccccgag cggctcagag atagcgggag atggccatcg ccaggatggc
     1801 gacgacgacc aggatggtca tgaccgggat caggcgatct cgggctgcca tcgtctggcc
     1861 catctctgcc cccacgtctt caccctgcgc ggcgcgtgca ccaagtgcgg ccaacttctc
     1921 gtttgccggg cggagctgga acatgccaac gatccacagc gcgatggccg caacgccacc
     1981 gatggcaaag ccggccatag ccgcgttgaa agggattacc tcgccgaagt cgtgccagta
     2041 gaggagtatc ccgctgagga tcgtgatcac ccctgccgca cccgctatct cccctaactt
     2101 cgcctccccg gtgagacgac ccatgaacgg cccggcagcg ggccccactg ccttagcggt
     2161 cggaatcaga aatcgaagca gaatcaggat gcttccggcc cagaaaatgc ctgcaccaac
     2221 gtggagcacc cgcaacacga ttacgtagac gttcatatgc gatcagtatt gccatggccg
     2281 ccggcgcgtg ctggcgatcg tgatgtcggt aggttgggcc gatgtcgtca ttgatgacca
     2341 acccgtggag atcgaccggt ctccctggat cctcaggcga cgaggtcagc gtcaacccct
     2401 cgaaggcgat tgacctcctt gcccgttgtc ccgcttctgg cgtcacccct ctgctcgacc
     2461 tgcccgaagt ggcccgggac ctcgaggtcg accatgtctg gatcaaggac gaacgcaacc
     2521 gcatggggct gggtagcttc aaggcactgg gagcggccta tgcgatcgcc catgatgcag
     2581 acgttcggct cgggggagac tgggatcagt ctccacatga cctggccaac attttggccg
     2641 acgtgactta cacagccgcc agtgctggga accatggcct ctccgtcgcc gcgggggccc
     2701 gggtgt
//
LOCUS       DBNHXU010000313         2978 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_510787, whole genome shotgun sequence.
ACCESSION   DBNHXU010000313 DBNHXU010000000
VERSION     DBNHXU010000313.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2978)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2978
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_510787"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC161X_07815"
     CDS             complement(<1..523)
                     /locus_tag="AC161X_07815"
                     /inference="COORDINATES: protein motif:HMM:NF024867.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989494.1"
                     /translation="MRPRRLVLDGFLAYRQRVEIDFTDADLFVLSGPTGAGKSSVIDG
                     MIFALYGTIPRLGHKGAVAPVISAQADLARVSFEFSVGDDTYLAARLVKRSNAGATTP
                     EATLERVGGEVLARGADEVTSRVIELLGLTYEQFTKAVVLPQGAFADFLTDKPSERQA
                     LLRALLDIGLFEKV"
     gene            complement(520..1650)
                     /locus_tag="AC161X_07820"
     CDS             complement(520..1650)
                     /locus_tag="AC161X_07820"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0004529 - DNA exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HIR4989495.1"
                     /translation="MRLLHTSDWHVGRKMRGRSRSGEHRAVLAEIAAIADDRKVDATL
                     VAGDLFDISSPSPEDEAIVYRALLDLAEVGPVLVVGGNHDNAARLDAIKPLLDLGRIL
                     VVPRPAPPGDGGVATLESLGLRAAFIPFVSQRGIIKAEQIMDLDPDQHVQTYEQRLRR
                     IIEALTADMGMDTVNIAIGHAFVYGATTGGGERESHILGYDIPPLAFPGHLSYVALGH
                     LHRQQRIPAPAPIWYSGSPIQLDFGEVGDQKGVLIVEAEAGKPASVEQVPIEGGTRLV
                     QLAGTLEQVIAMAPDVEGAYVKVVLDEKTRAGLNEEVRAAIPGAVDVIIARAEDGRPA
                     ERVTRAGRSPVDLFATYLEHKGVDDPAVLDLFRELEEEAVPL"
     gene            complement(1713..>2978)
                     /locus_tag="AC161X_07825"
     CDS             complement(1713..>2978)
                     /locus_tag="AC161X_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4989496.1"
                     /translation="PLYANFEFLNGQRGAHVNISGVSGVATKTSYATFLLHSLFTSDV
                     LGTAAVNTKALIFNVKGEDLLHIDRPNVSLDDDARRRYKDLGLPARPFNSVGLFAPPR
                     AGSGSAVAEVSSRSDITSFFWTLSEFCHQDLMVFLFADAEDERAQYTMVVYNVMAQLR
                     HSEEGDGGSVRIEGETVRTFRELVDLITRKVQQDDSNSDWAGPHIGQGTVNAFVRRLQ
                     GSVRHVERLIRGDIPNPARHSIDVQGNQVTVVDLHNLNDRAKRFVVGVTVRRAFEDKE
                     DAGQSEELLFLVLDELNKYAPREGFSPIKEVLLDVAERGRSLGVILIGAQQTASEVER
                     RIIANSSIRIVGRLDSAESSREEYGFLPAAQRQRATILKPGTMLLAQPELPVPLVLEF
                     PFPAWATRASEAGAMPISGDEPADPFQDL"
BASE COUNT          553 a          951 c          927 g          547 t
ORIGIN      
        1 tcaccttctc gaacaagccg atgtcgagca gcgcccggag caaggcctgg cgctcactcg
       61 gcttgtcggt caggaaatcg gcgaaagctc cctgaggcag caccacagcc ttggtgaact
      121 gttcgtaagt gagcccgagg agctcgatga cgcggctcgt gacctcgtcg gcgccgcgag
      181 cgaggacctc gccaccgact ctctccagag tggcctcggg ggtggtcgcc ccggcgttgc
      241 tgcgtttcac gagacgtgcc gccaggtagg tgtcgtcgcc tacggagaac tcgaacgaca
      301 cccgtgccag atcggcctgg gccgaaatca ccggggcgac cgcccccttg tgaccgagcc
      361 ggggaatggt cccgtagagg gcaaagatca tcccgtcgat cacgctcgac ttgcccgctc
      421 cggtagggcc ggagagaacg aagaggtcag catctgtgaa gtcgatctcg acccgttgcc
      481 ggtaggccaa aaagccatcc aggaccagcc gtcgcggcct caaagcggaa ccgcctcttc
      541 ctcgagctcg cggaaaaggt cgagcaccgc aggatcgtcg actcctttgt gctccaagta
      601 ggtggcgaac agatcgacgg gggatcgacc cgctctggtc acccgttccg cgggccgacc
      661 atcttcggct cgggcaatga tcacgtccac tgcccccggg atcgcggcac gaacctcttc
      721 gttgagcccg gcccgcgtct tctcgtcaag cacaaccttg acataggcgc cctccacgtc
      781 aggcgccatg gcaatgacct gctccaaggt tcctgcaagt tggaccaatc tggtgccgcc
      841 ttcgattggc acctgttcga ccgaagccgg cttgcccgct tccgcctcga cgatgagcac
      901 gcccttttgg tcaccaacct cgccgaagtc gagctggatc ggcgatccgc tgtaccagat
      961 cggtgccgga gcaggaatcc tctgctgcct atgtaggtgc ccgagagcca cataggagag
     1021 atgacctggg aacgccagcg gcgggatgtc gtatcccagg atgtgggact cgcgctcgcc
     1081 tccgccggtg gtggcgccat acacaaacgc atgtccgata gcgatgttga ctgtgtccat
     1141 gcccatgtcc gcggtgaggg cttcgatgat cctcctcagc cgttgctcgt aggtctgaac
     1201 gtgctggtca ggatcgagat ccatgatctg ctccgccttg atgatccccc gctgcgatac
     1261 gaacgggatg aaagcggcgc ggagcccaag cgattcgagt gtggcaactc cgccatcgcc
     1321 cggtggggcg ggccgaggca caaccaggat tcggcccaga tcgagcaacg gtttgatggc
     1381 gtcgaggcga gcggcattgt cgtgatttcc accgaccacg agcaccggcc cgacctcagc
     1441 caggtcgagc agggcccggt aaacgatcgc ctcatcctcc ggcgacgggg aagagatatc
     1501 gaacaagtcg ccggcgacca gggtggcatc gaccttgcgg tcatcggcga tcgctgcgat
     1561 ctcggcgagc actgcccggt gctcgccaga gcgggaccgc ccgcgcatct tcctgcccac
     1621 gtgccaatcg gaggtgtgga gcagtctcac ttgacctgat tcaccgtcat caatccgatc
     1681 gtaggaccgg ggtgggacag attccgggac tcctagaggt cctggaacgg gtcggcgggc
     1741 tcatcacccg atatcggcat ggcccccgcc tcggaagctc tggtcgccca ggcgggaaag
     1801 ggaaattcga gaaccagcgg caccggaagc tccggctgag cgaggagcat ggtgcccggc
     1861 ttgaggatgg tggctctttg ccgctgcgca gcggggagga agccatattc ctcccgtgac
     1921 gactcggcag agtccaatcg gccgacgatc cggatcgagg agttggcaat gatccgacgc
     1981 tcgacttcgg aggctgtctg ctgagcaccg atcaggatca cccccagtga tctccctcgt
     2041 tcggccacat cgagaagcac ctcttttatc gggctgaagc cctccctcgg cgcgtatttg
     2101 ttcaattcgt ccaggaccag gaacaacaac tcctcggact gtccggcgtc ctctttgtcc
     2161 tcgaaggcgc gcctcaccgt gacccccacg acgaaccgct tcgcccggtc gttcaagttg
     2221 tggagatcga caacggttac ctgattgccc tggacatcga tgctgtgacg cgccggattg
     2281 gggatgtccc cccgaatcag acgctcaacg tggcgcaccg agccctgaag tcgccgcacg
     2341 aaagcgttga ccgttccctg cccgatgtgg ggcccggccc agtcgctgtt cgagtcgtcc
     2401 tgctgcacct ttctggtgat gaggtcgacg agttcgcgga aggtgcgcac cgtttcgcct
     2461 tcgatccgga ctgatccacc gtcgccttcc tcggagtgcc tcagttgggc catgacgttg
     2521 tacaccacca tcgtgtactg cgcccgttcg tcttcggcgt ctgcgaagag aaagaccatc
     2581 aagtcctggt ggcagaactc ggagagagtc cagaagaagg aagtgatgtc ggagcgggag
     2641 ctcacttcgg caaccgcact gccggatccc gccctcggcg gggcgaacag cccgaccgag
     2701 ttgaacggcc gtgccgggag gccaaggtcc ttgtagcgcc gtctggcatc gtcatcgagg
     2761 ctgacgttgg gacgatcgat gtggagcagg tcttcgccct tcacgttgaa gatcaatgcc
     2821 ttggtgttga ccgccgccgt ccccagcaca tccgaggtga acaacgagtg gagcaggaac
     2881 gtggcgtaac tggtcttggt ggccaccccc gacacccccg agatgttgac atgggctccc
     2941 ctttgcccat tgaggaactc gaagttggcg tagagcgg
//
LOCUS       DBNHXU010000314         2772 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2892060, whole genome shotgun sequence.
ACCESSION   DBNHXU010000314 DBNHXU010000000
VERSION     DBNHXU010000314.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2892060"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..272
                     /locus_tag="AC161X_07830"
     CDS             <1..272
                     /locus_tag="AC161X_07830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989497.1"
                     /translation="QPDTVRQMLTPHGFDDNGVEAYGYGMELVVVDDRVTVYGHGGAD
                     PGVSTMASHYVDAGVTVVVLCNQDRGSWATVQKITEMLDLDDPRE"
     gene            complement(276..1757)
                     /locus_tag="AC161X_07835"
     CDS             complement(276..1757)
                     /locus_tag="AC161X_07835"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015443116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     (non-electrogenic) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HIR4989498.1"
                     /translation="MTFDVDYLAFAPEFVIIATLLVVFALDLVLPRAQKYWTATAAVA
                     GTALAAVPLVFLAVNGETISMFDGSYVVDEFALVLKGLFVVAAYLVFLMSHHYIESDR
                     YYQGEYYFLLLCSVLGSMVMASARDIIILFVALELTTGPLYLLAGWRKGDVKSNEASM
                     KYFILGVLSTALLLYGLSFIYGLTGEVTFSGIAEATAGMAGEPALIVAVLFVIAGFGF
                     KIAAVPFHFWAPDTYEGAPTPVTAYLSVNSKAAGFVAMLLMMYLALPSVSEIWAPALW
                     VLAALSMTIGNLSALRQTNIVRLLAYSSVAQAGFMLVPFASAAVVGADLEEAFSATII
                     YLTIYAVMNLGAFAVVIAGARKTRSGELRAWSGLAQADTRLGVLVAVFFFSLAGIPPL
                     AGWFAKFAMFRSVMIAGGTATVVLAVIAALNAVVAIYYYARVVKTVWIDDPIGEFEDD
                     ASAAPAGSLRLALGLSLAFTVLVGFYPTIATFVGDASRVLASG"
     gene            complement(1762..>2772)
                     /locus_tag="AC161X_07840"
     CDS             complement(1762..>2772)
                     /locus_tag="AC161X_07840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012225139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045271 - respiratory chain complex I
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048039 - ubiquinone binding [Evidence
                     IEA];
                     GO_process: GO:0015990 - electron transport coupled proton
                     transport [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 4 family protein"
                     /protein_id="HIR4989499.1"
                     /translation="LALYFKSGEVSGVSTFDMVELTKLGAGGAFTGTFAFLVFGAMFL
                     GFAVKVPMWPFHTWLPDAHTAAPTVGSVLLAAILLKLGSYGFIRISLPILPQEAANWA
                     PAIAILAVIGIIYGSLACLAQTDMKRLIAFSSVGHMGFVMLGIATMTDIGINAAIIGM
                     VAHGLITGLLFFLAGSMSHRYHTREMSRLGGNIKLMPVMGGILAFTAMASLGLPGLAG
                     FWGEFMSLVGSYNPLAGLPLGVFRTAMVLGAIGTVLTAGYMLYMLQQVNLGEPGAEWE
                     GKEFHDVDRFELTAWAPLIVLILVIGFYPNLIFDATTDTVTSMVNTVLDTDAVASLGS
                     GG"
BASE COUNT          618 a          874 c          883 g          397 t
ORIGIN      
        1 tccaacctga cacggtgcgc cagatgctga ccccgcatgg gttcgacgac aacggggtcg
       61 aggcctacgg ctacgggatg gagttggtgg tggtcgacga tcgggtgacg gtctacgggc
      121 acggtggagc cgatccgggg gtgtcgacga tggcgtcgca ctatgtcgat gccggggtca
      181 ccgtggtcgt gctctgtaac caggaccgtg ggtcatgggc caccgtgcag aagatcacgg
      241 aaatgctgga cctcgacgac ccccgcgagt agcactcaac cgcttgccaa tacgcgtgag
      301 gcgtctccga cgaaggtggc aatcgtcggg tagaagccga ccaggaccgt gaatgccaac
      361 gacagcccga gggcgagtcg cagcgaaccg gcaggagcgg ccgatgcgtc atcctcgaac
      421 tccccgatcg ggtcgtcgat ccagaccgtt ttcacgaccc gggcgtagta gtagatggca
      481 accaccgcgt tgagagctgc gatcaccgcc aggaccaccg tggcagtccc tccggcgatc
      541 atcaccgagc ggaacatcgc gaacttggcg aaccacccag cgaggggtgg gatcccggcc
      601 aagctgaaga aaaacaccgc gaccaacacc ccaaggcggg tgtccgcctg ggcgagcccg
      661 ctccatgccc gaagctctcc ggatcgggtc ttgcgggcac cggcgatcac cactgcgaag
      721 gcgcccaagt tcataaccgc gtagatcgtg agatagatga tggtcgccga aaacgcctcc
      781 tccaggtcgg cgcccacgac ggccgcagag gcgaagggca ccagcatgaa accggcctgc
      841 gccaccgacg agtaggcgag cagacgaaca atgttggtct gtctcagcgc agagagattc
      901 ccgatcgtca tcgacaacgc agccaggacc cacagcgccg gggcccagat ttcggagacc
      961 gagggcagcg ccagatacat catgaggagc atcgccacga agccggcagc cttcgagttg
     1021 accgacaaat atgccgtcac cggagtcgga gccccttcat acgtgtcggg cgcccagaag
     1081 tggaagggca cagcggcgat cttgaagccg aagccagcga tgacgaagag gaccgccacg
     1141 atcaaggccg gctcacccgc catgcctgcg gtcgcctccg caatcccgga gaacgtcacc
     1201 tcgccggtca gtccatagat gaacgagagg ccgtagagga ggagcgccgt cgagagaact
     1261 ccgaggatga agtacttcat cgaagcctcg ttcgacttca catcaccctt ccgccaaccc
     1321 gccagcaggt agagcggccc ggtggtcaat tcgagggcaa cgaacaggat gatgatgtcg
     1381 cgggcggacg ccatgaccat cgatccgaga accgagcaga gcaacaggaa gtagtactcc
     1441 ccctggtagt agcggtcgga ctcgatgtag tggtgggaca tcaggaacac caggtaggcc
     1501 gccaccacga aaaggccttt gagtaccaag gcgaactcgt cgaccacgta ggagccgtcg
     1561 aacatcgaaa tcgtctcgcc attgacggcg aggaacacca gagggaccgc cgctagagcg
     1621 gtcccggcca cggccgcggt cgcggtccag tacttctggg cccggggtag caccaggtcg
     1681 agggcgaaga cgaccaacag tgtcgcgatg atcacgaact caggcgcaaa cgccaggtaa
     1741 tcaacgtcga aggtcacacg ctcacccgcc cgaccccagc gaagcgaccg cgtccgtgtc
     1801 gagcacggtg ttcaccatcg acgtcaccgt gtctgtagtc gcgtcgaaga tgaggttggg
     1861 atagaacccg atcaccaata tcagcacgat cagtggggcc caggcggtca actcgaagcg
     1921 gtcgacgtcg tggaactcct tcccctccca ctccgctccc ggctcaccga ggttgacctg
     1981 ctgcaacatg tagagcatgt atcccgcggt tagaacggtc ccaatggccc cgagcaccat
     2041 cgcggtccgg aacacgccga gcggcaggcc cgccaggggg ttgtaggagc cgaccagtga
     2101 catgaactcg ccccagaagc ccgccaaccc ggggaggcca agcgacgcca tggcggtgaa
     2161 cgccaggatc ccacccatca ccggcatcaa cttgatgttc ccgccgaggc gggacatttc
     2221 ccgggtgtga tagcgatggc tcatcgagcc ggcgaggaag aagagaaggc cggtgatcag
     2281 gccgtgggcc accatgccga tgatggcggc gttgatcccg atgtcggtca tcgtggcgat
     2341 ccccagcatc acaaagccca tgtgccccac cgacgagaag gcgatgaggc gtttcatgtc
     2401 ggtctgggcg agacaggcca gcgagccgta gatgatcccg atcaccgcca ggatcgcgat
     2461 cgccggagcc cagtttgccg cctcctgggg aaggatcggg agcgagatcc ggatgaaccc
     2521 gtatgagccc agcttcagga ggattgctgc caggagcacc gaaccgacgg tcggggccgc
     2581 ggtatgggca tcggggagcc aggtatggaa cggccacatc ggcaccttga ctgcaaaccc
     2641 gaggaacatc gccccgaaga ccaggaaggc aaaggtcccg gtgaaggcac ccccggcgcc
     2701 gagcttggtc agctcgacca tgtcgaatgt gctcaccccg ctcacctcac cggacttgaa
     2761 gtagagcgcc ag
//
LOCUS       DBNHXU010000315         4220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1107699, whole genome shotgun sequence.
ACCESSION   DBNHXU010000315 DBNHXU010000000
VERSION     DBNHXU010000315.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1107699"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..236
                     /locus_tag="AC161X_07845"
     CDS             <1..236
                     /locus_tag="AC161X_07845"
                     /inference="COORDINATES: protein motif:HMM:NF024731.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="magnesium chelatase subunit ChlI family protein"
                     /protein_id="HIR4989500.1"
                     /translation="QSSRGQINRHLTPSAFPESVGARSLVASAVGSALLTARGADRVR
                     RVARTIADLAGVEQVEEEQMAEAVGLRGEWRDD"
     gene            229..1053
                     /gene="dprA"
                     /locus_tag="AC161X_07850"
     CDS             229..1053
                     /gene="dprA"
                     /locus_tag="AC161X_07850"
                     /inference="COORDINATES: protein motif:HMM:NF014533.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HIR4989501.1"
                     /translation="MTEHRLVKPGDPTYPAGLFDLEEPPSLYVLGQTTTAPAVAVVGT
                     RRCTRYGLSLAEGFGRALAQAGWTTVSGLARGIDASAHRGTLGGRGEAVAVLGSGIDV
                     VYPAENRELYHSIVAGAGAVISEYPPGTPPDRWRFPARNRLIAAIASAVVVVEASNKG
                     GALITARLAAELGRPVFAVPGDVDRAASVGCNLLIRDGAIPALGVEDLIEELQLVLGK
                     PPRSPEPAIGDIPATGVALDDLPGIWGCAPAEALARLARMEVAGEIRRSGDRVVPI"
     gene            1091..1978
                     /locus_tag="AC161X_07855"
     CDS             1091..1978
                     /locus_tag="AC161X_07855"
                     /inference="COORDINATES: protein motif:HMM:NF012798.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HIR4989502.1"
                     /translation="MHLPDMPAWAAAPVDDYLARLREQRDLSPNTVDAYRRDLTQFFD
                     YCDRNGVERIDDPQRLHARRYLAFLDTLGYSRRSATRKASAVRSFYNDAGRRGTATVN
                     PFEGVARPRLDRPLPHALPARTVVHAIESIDTSSPQGLRDRAVLDTLYSTGLRISELA
                     SLTVADVNGDMITVVGKGRKTRRVPVGRPAQESLRRYVDDGRPHLASSEAGRWLWVGL
                     RGGRLDTRGLRKVVKDRMATFPHALRHSFATHLLEGGADLRVVQDLLGHTDLATTQIY
                     TAVTRQHLRGTYDRSHPRA"
     gene            1953..2732
                     /locus_tag="AC161X_07860"
     CDS             1953..2732
                     /locus_tag="AC161X_07860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018561721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed RNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FliA/WhiG family RNA polymerase sigma factor"
                     /protein_id="HIR4989503.1"
                     /translation="MTEATQGPETNQLEALWGRFKASADADARERLILHYSPLVKYVA
                     GRVGAGLPRSVDQNDLASYGLFGLIDAIDKFELERGFKFETYAINRIKGAILDELRAL
                     DWVPRSVRSRARQIEAGIAEMEHSLQRAPTDQELADFLEMAVDDLQSALAEIGRSGIA
                     ALDEFVGNESSTSVGEMLADPTGISPEIAFQSEETRRGLVDAINRLPNRERLVITLYY
                     YEGMTLAEIGDVLGVTESRVCQIHAKTMMSLRNRLGEPSWG"
     gene            2741..3223
                     /locus_tag="AC161X_07865"
     CDS             2741..3223
                     /locus_tag="AC161X_07865"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4989504.1"
                     /translation="MTDRIRHRYLIDLDGWIISGTDPARGEIRSPSLEDADGLSQLML
                     DAYVGTIDYDGENLADARVEVDEYFSSEPLLECSRVVEIGGELAAAALLTLWDGAPLI
                     GYVMTRSLRKGQGLARVVLVSALDCLARTSNRQVHAFITEGNTASERLFASLGAVLHD
                     "
     gene            3397..4107
                     /gene="rpsB"
                     /locus_tag="AC161X_07870"
     CDS             3397..4107
                     /gene="rpsB"
                     /locus_tag="AC161X_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008587850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HIR4989505.1"
                     /translation="MKQLLEAGVHFGHQTRRWNPKMRPYIYGERNGIHIIDLRQTLEQ
                     INDATAYVKDLVAGGGTVLFVGTKKQAQAAVAEHAGRSGMPYVNFRWLGGMLTNFATI
                     QKRIFYMRELRRLEESGEINSLPKKERLKLRRELGKLEQNLGGVVDLQRVPDAVFVID
                     VNAETTAVTEASRLGLPVIALVDSNCDPDLIEYVIPGNDDAIRAADLIAGALADAALE
                     GRELATAKTAKADDTEES"
BASE COUNT          779 a         1342 c         1353 g          746 t
ORIGIN      
        1 tccagagttc cagagggcaa atcaaccgcc acctcactcc gtctgctttt cccgagtctg
       61 ttggggcccg ctccttggtg gcatcggccg tggggagcgc cctgctcacg gctcgtgggg
      121 ccgaccgggt gcgccgggtg gctcggacca ttgccgacct ggcgggggtc gaacaggtcg
      181 aggaggagca aatggccgag gcggtcggcc tccgaggcga atggcgcgat gactgagcac
      241 cggctcgtca agccaggcga ccccacctac ccggcagggc tcttcgatct cgaagagcca
      301 ccctccctgt acgtactagg gcagaccact accgctcctg cggtagccgt ggtcgggacc
      361 aggcgatgca cccgctacgg cctgagcctt gcggaggggt tcggacgcgc cctcgcccag
      421 gcaggctgga ccactgtcag cggccttgct cgcgggattg atgcctccgc ccaccgcggc
      481 acccttggcg gccgtggcga agcggtcgca gtgctggggt ccggcatcga tgttgtctat
      541 ccggccgaga accgggagct atatcactcg atagtcgctg gagcaggggc ggtcatctct
      601 gagtacccac cgggcacacc accagatcga tggcggtttc cggcgagaaa ccggctgatt
      661 gcagcgatcg cctcggcggt ggtggtggtc gaggcaagca acaaaggagg tgcgttgatc
      721 acggcgcgtc tcgctgccga gttgggacgc cccgtgtttg cggttcccgg ggacgtcgac
      781 cgggcggcat cggttgggtg caacctcctg attcgagacg gggcgattcc ggcactggga
      841 gtcgaagacc tgatcgagga gttgcagttg gtgctgggaa agcctccaag gtcgccggag
      901 ccggcgattg gagatatccc tgccactggg gtggccctcg acgacctgcc cgggatttgg
      961 ggttgtgccc cggctgaggc actcgcccgg ctggcgcgga tggaggtggc gggcgaaatc
     1021 cgccgttcag gcgaccgggt ggttccgatc tgaactggtt ctcgcgaaat ctcgctttag
     1081 gatcagagaa gtgcatctcc ccgacatgcc ggcgtgggca gcagccccgg tcgacgacta
     1141 tctggcccgc ctccgcgagc agcgcgacct atcacccaac acggtggacg cctaccgccg
     1201 cgatctgacc caattcttcg attactgcga tcgaaacggg gtcgagcgga ttgacgaccc
     1261 ccagaggctt cacgcacgcc ggtatctggc atttctggac accctcggtt actcgagacg
     1321 gtcggcgacc cgcaaagcat cggcggtgcg gtcgttctac aacgatgccg gcagacgtgg
     1381 cactgccacc gtcaacccgt tcgaaggagt ggccaggccc cgtctcgatc gaccgttgcc
     1441 ccacgcccta cccgccagga ccgtggtgca cgccatcgag tcgatcgaca cttcgtcgcc
     1501 gcagggactc cgcgaccgcg ccgtcctcga caccctctat tcaacgggac tccgcatctc
     1561 ggaactggcc tcgttgacgg tcgccgacgt caacggagac atgatcacgg tcgtcggaaa
     1621 ggggaggaag acccgacggg tcccggtggg ccgcccggca caggagtcgc tgcgacgcta
     1681 tgtcgatgac ggacggccac atctggcttc ctcggaggcc ggacgatggc tttgggtcgg
     1741 tctcaggggc ggtcggctcg acaccagggg cttgcgcaaa gtggtgaagg accggatggc
     1801 caccttcccc cacgccctac gccacagttt cgccacccat ctgctcgaag gtggcgccga
     1861 tttgcgggtt gtacaggatc tcctgggaca tacagacctg gcgacaaccc agatctacac
     1921 tgccgtgacc cgtcagcacc tacgagggac ttatgaccga agccacccaa gggcctgaga
     1981 caaatcagct cgaagcccta tgggggcgct tcaaggcctc ggccgatgct gacgctcgcg
     2041 agcgtttgat cctccattac tcgcccctgg tcaaatacgt cgccggtcgg gtcggcgccg
     2101 gcctgccccg ctcggtggat cagaacgatc tggccagtta tggccttttc gggctgatcg
     2161 atgccatcga caagttcgag ttggagcggg gtttcaaatt cgagacctat gcgatcaacc
     2221 ggatcaaagg ggcgattctc gacgagctgc gggccctgga ctgggtgccg cgctcggttc
     2281 ggtcccgagc ccgtcagatc gaggcgggga tcgccgagat ggagcacagc ctgcagcggg
     2341 ctcccactga ccaggagctt gcggatttcc tcgagatggc cgtcgacgat ctgcagagtg
     2401 ccctcgccga gatcgggcgc tccggcatcg ccgccctcga cgaattcgta ggaaacgaat
     2461 cgtcgacctc ggttggcgag atgctcgccg accccacggg gatctcccct gagatcgctt
     2521 ttcaatccga ggagacccgt cgcggcctcg tcgacgcaat caatcgcctc cccaaccggg
     2581 agcggctagt gatcaccctc tactactacg aggggatgac gctggccgag atcggagatg
     2641 tgctcggagt caccgagtcc cgtgtctgcc agatccacgc caagacgatg atgtcgcttc
     2701 gcaaccggct cggcgagcct tcttggggtt gagtggcccg gtgaccgaca gaatccgaca
     2761 tcgatacctg atcgatctcg atgggtggat catctccggc actgacccgg ctcgaggcga
     2821 aatcagatca ccgtctctcg aggacgccga cgggctttcc cagctcatgt tggacgccta
     2881 cgtcggaacc atcgactacg acggagagaa cctcgccgac gccagggtcg aagtcgacga
     2941 atacttctcg tcggagcccc tcctcgaatg ctcgcgggtg gtcgaaatcg ggggcgagct
     3001 tgctgccgca gcgctgttga cgctctggga tggggcgccc ctcatcggct atgtcatgac
     3061 ccggtccctg cgcaaaggcc agggccttgc cagggtggtc ctggtttcgg cactcgactg
     3121 cctggcacgc acttcgaatc ggcaggtcca cgctttcatt accgagggca acaccgcgtc
     3181 ggagcgccta tttgcctcac tcggtgccgt ccttcacgac taaccccgac tcgattaccg
     3241 gacgacaggc cacggcctta caatcgcctc atcacgcacg ctcgctgacc ctcactggtc
     3301 gggcgcgtcc cgaaagagag tgactcgaaa gagtcgggtg gctttccacc ggcgggcgga
     3361 ggtacaacca gaaaggaatc actggtggct gtcaccatga agcaactctt ggaggccggc
     3421 gtccatttcg ggcatcagac ccgacgctgg aatccaaaaa tgcgccccta catatacggg
     3481 gagcgcaacg ggatacacat catcgacctc cggcagaccc tggagcagat caacgacgcg
     3541 acggcctacg tcaaggacct ggttgccggc gggggaacgg tgctcttcgt gggcaccaag
     3601 aagcaggcgc aagccgccgt cgccgagcac gccggccggt ccggtatgcc ctatgtcaac
     3661 ttcaggtggc ttggtgggat gctcaccaac tttgccacga tccagaagcg catcttctac
     3721 atgcgtgagc tgcgtcgtct cgaagagagc ggtgagatca actcacttcc caagaaggag
     3781 cgtctcaaac tgcggcgtga gttgggcaag ctcgagcaga acctcggcgg tgtcgtcgat
     3841 ctgcagcggg tccccgacgc cgtgttcgtg atcgatgtca atgcggagac gacagcagtg
     3901 accgaagcct cgaggttggg cctgccggta atcgccctcg tcgatagcaa ctgtgatccg
     3961 gatctgatcg agtacgtcat tccggggaac gacgacgcga tccgggctgc cgatctcatt
     4021 gctggcgccc tcgccgacgc tgctctcgaa gggcgggagc tggctactgc caagaccgcc
     4081 aaagcagacg acaccgagga atcctgaggc tgagcatggc tgagttcact gccaaagaca
     4141 ttcagaagct gagagtcgac gccggggttg ggatgatgga cgccaagaag gccctcgccg
     4201 acgccgaagg ggacttcgac
//
LOCUS       DBNHXU010000316         6110 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3288108, whole genome shotgun sequence.
ACCESSION   DBNHXU010000316 DBNHXU010000000
VERSION     DBNHXU010000316.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6110)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6110
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3288108"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..714
                     /locus_tag="AC161X_07875"
     CDS             <1..714
                     /locus_tag="AC161X_07875"
                     /inference="COORDINATES: protein motif:HMM:NF014893.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family protein"
                     /protein_id="HIR4989506.1"
                     /translation="AAYLQSWGGPNTPLLWRFQGEVAGSGAHGDLNAHVIDAVRFLTG
                     EEIVNVDGAIEHTFIEERALLEGGAGGEIAGAGASTSAGRGKSTVDDAVLFIGRLSGG
                     GLASFEATRLATGYHNANRLEIHGERGAIRFDFERMNELGFYDASDDGATQGWTTIDV
                     TRGGDGHPYADAWWPDSHGLGYENTFVNQAADMLKVIGGGDPVVPLPDFADALETQKV
                     LTAALISAREHAPIDVTEL"
     gene            735..1979
                     /locus_tag="AC161X_07880"
     CDS             735..1979
                     /locus_tag="AC161X_07880"
                     /inference="COORDINATES: protein motif:HMM:NF013429.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase family
                     protein"
                     /protein_id="HIR4989507.1"
                     /translation="MSTAAPFEPTFMPVGVLTAALQELTPREKRDPDPDLAIEEWLEF
                     ASELGADCIELSAAVHPDLADVPPEAMLDPVANTLDLRQPFDQARARRVQAAVDATGV
                     RIVDIAYFDNLLHQDPDIRRQKHEFMLRVMDTAVLLGAPAVAGFVGRDQTKSMDQNLI
                     EYEESFIPLLQAAKDRGLMYRVEQCPMPGWTPSDNYHNNIGYTPAMWIALHKIAEKHG
                     LGDQFRIHYDPSHSVLMGQDTRSMFQYLKDEGYAFLIGGLHVKGQVIDSRGVSAWGYG
                     GQTVQRGDWIDGEISPNPADHAKAWLIQTVLAEHELPGTAKHDPLAYLQNRSVDWLDH
                     QLALRELLDLDVATTPLIVEHEYPLARVQDKDNLRPILEGSIAFTRYIDQAAAAMYSL
                     QNEVLASQGIPVQGVGREAYRS"
     gene            2114..4168
                     /locus_tag="AC161X_07885"
     CDS             2114..4168
                     /locus_tag="AC161X_07885"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006311832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HIR4989508.1"
                     /translation="MSDLILYAALAAGLAALVLASYYRRSVLAAPRGNQRMIEISDAI
                     REGAMAFLRREYTWILIFVAAMTALIAFLLDWGWPWGAAAYVFGAALSAGAGYAGMRI
                     ATAANSRTTEAARTGGVSAALAIAFRGGAVAGFTIAGFGLLGVGLGFLIFDRVLELDS
                     WAESITAIALGGSSIAIFARVGGGIFTKAADIGADLVGKVEAGIPEDDPRNPAVIADN
                     VGDNVGDVAGMGADLFESYVGALVAPIAYAAIVFRGSGFIEETILFPLAIAAIGMLSA
                     IVGSFLVRANDEGHLSAALHRGSNFALVVTALGALGLSYWIFGDVIEHPIGVWLAIIA
                     GLVVGWLVGKISEWFTSDNHKTVKEIARQAQTGPATVIITGLAEGMRSAAFSVIVVAT
                     GIGAAFWAGDWAMGAGGGLYGVAIAAIGVLATLGMTISMDAYGPIADNAGGIAEMAQL
                     APEVRHATDSLDALGNTTAAVAKGLAIASAAVTALALFTAFIEAVGLNAIDLTRADTT
                     IGLLLGAMFPFLFAAMTMSAVGRTANRMIDEVRRQFREIPGLREGDPKARPEYAKCVD
                     IATTGALREMVIPATMAVALPLVVGFVDIEALGGLLGGALVTGFLLAIFMSNAGGAWD
                     NAKKFIETGAFGGKGSDAHKAAVIGDTVGDPFKDTAGPSMNIVIKVMAIVSLIFASAF
                     IS"
     gene            4170..5402
                     /locus_tag="AC161X_07890"
     CDS             4170..5402
                     /locus_tag="AC161X_07890"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HIR4989509.1"
                     /translation="MPEVLLIRHAESETNRSGVWAGRSDGPLSSAGEASLRPLAERFS
                     NQKFELVMSSPLERATRTAAAISPQVEISEEFTEIDIGRWEGRTREEVLAADDDLIRE
                     ARVNRDLAWGGTGESLRDAELRAWLALDELAGRLGERERAVVVTHGGLLNAVLHRYLA
                     GSSRRVHAFAGNTSVTRLAWTHDRPRLASFNDQGHLGPRSRVVDEELAADRPVLALIR
                     HGQTQANIEGRWQGHGDWDLDERGHTQAAALRDWYGTHDLVYSSPLSRAQSTAGYLAN
                     NGVVTVEALKELGMGKWEGLTSPEVIEQWPDLMDDIFRDGVDLRRGENGESWGELAHR
                     VRNAVHSLRPARGVPTVAVAHGGAIRSYISSLTATTDTHAESLHTPRNTSVTHLSFHD
                     DGPLILDYAVATHLETLS"
     gene            5412..5993
                     /locus_tag="AC161X_07895"
     CDS             5412..5993
                     /locus_tag="AC161X_07895"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4989510.1"
                     /translation="MSEYDSRRYALVNRWDPQHLKTVDRLLPLNPGDRVLEVGCGSGH
                     LTKRLAARGIDVVGIDANPNAREVAGTDRVLHMRAEQLGFEDKEFDAVISVHAIEHIP
                     PLDEAVAEMARVLQPQGRALLIYPAEPIQGLYAIPTSVILYGTPFKAREVHCHKLWPS
                     KVRRIVEPFGLTETHHEFNLWKSPQFVSLFTRF"
BASE COUNT         1096 a         1878 c         1947 g         1189 t
ORIGIN      
        1 gcggcctacc tccagagttg gggtggtccg aacacgccac tcctgtggcg cttccaaggt
       61 gaagtggctg gttcgggcgc ccacggtgac ctcaatgcgc acgtcatcga tgccgttcgg
      121 ttcctcaccg gggaggagat cgtcaatgtc gatggggcga tcgagcacac gttcatcgag
      181 gagagggcgc tcctcgaagg aggcgccgga ggtgagattg ccggagcggg tgcctccaca
      241 tcagcgggcc gaggtaagag cactgtcgat gatgcggtcc tcttcatcgg ccgactgtct
      301 ggtggggggc tggccagctt cgaagcgacg aggctggcga ccggctacca caatgcaaac
      361 cgtctcgaga tccacggcga acgaggagct attcggttcg acttcgagcg aatgaacgaa
      421 ctggggtttt atgacgccag tgacgacgga gccactcaag gttggaccac gattgatgtg
      481 acccggggtg gcgacggcca tccttatgcg gatgcctggt ggcccgacag tcacgggctg
      541 ggttatgaga acacattcgt caaccaggct gcggacatgc tcaaggtgat aggtggggga
      601 gatccagtgg ttccccttcc agactttgcc gacgccctcg aaacccagaa agtgctcacc
      661 gcggcgctga tatcggcgcg cgagcacgca ccaatcgacg tgaccgaact ctgacaccca
      721 atgagaggaa acaaatgagc accgctgctc ccttcgaacc aacgttcatg ccagttggcg
      781 tcttgaccgc ggcccttcaa gagctgacgc cacgtgaaaa gcgggatccc gatcccgacc
      841 tggccatcga ggaatggctc gagttcgcgt ccgaattagg tgccgactgc atcgagttgt
      901 ccgcagctgt ccatccggac ctcgccgacg ttccccccga ggccatgttg gatccggtgg
      961 ccaacaccct tgacctgcgc cagccgttcg accaagcccg agctcgtcgt gtccaagctg
     1021 ccgtcgacgc cacgggggtc cgcatcgtcg acatcgcgta cttcgacaat ctcctgcacc
     1081 aggaccctga catacggcgt cagaagcacg agttcatgtt gcgggtcatg gacactgccg
     1141 ttctcttggg ggccccggca gtggccggct tcgtgggccg cgatcagacc aagtcgatgg
     1201 accagaacct catcgaatac gaggagagct tcatccccct ccttcaggcg gccaaagacc
     1261 gtggactcat gtatcgcgtg gagcagtgtc cgatgccggg gtggactccc agtgacaact
     1321 accacaacaa catcggctac acgcccgcga tgtggattgc tctccacaaa atcgcggaga
     1381 agcacggctt aggcgatcag ttcaggattc attacgaccc gtctcactcg gtgctcatgg
     1441 gtcaggacac caggtcgatg ttccagtact tgaaggacga aggctatgca ttcctgatcg
     1501 gtgggctcca cgtcaagggt caggtgatcg acagccgagg agtttccgcc tggggttatg
     1561 ggggtcagac ggttcagcgt ggcgactgga tcgacggcga gatctccccc aatccagccg
     1621 atcatgccaa agcgtggctc atccaaaccg tgctggccga acacgagctg cccggaacgg
     1681 caaagcatga ccccctggcg tatctccaga accggtcggt tgactggctt gaccatcagt
     1741 tggcacttcg cgaactgctc gacctagatg tggcaactac tcctctgatt gtcgagcacg
     1801 agtacccgct ggcgcgcgtg caggacaagg acaaccttcg gccgatcctc gaagggtcga
     1861 ttgcgttcac ccgctacatc gaccaggcgg ccgccgcgat gtattcgctc cagaacgaag
     1921 tgcttgcgtc gcaaggtata ccggttcagg gagttgggcg agaggcctac cggagctgat
     1981 tcccggctga caagacgttg tcgcacgcca ggtgtggcgg ccttcgggag tggccgcgcg
     2041 gtgctgggcc actccgtccc gtccgtataa tccgcggcgc cggtcgtctg cctcatcaac
     2101 ggggtccttt tcgatgtcag acctgatcct ttacgccgcg ctcgctgcgg ggctcgctgc
     2161 cctggtgctg gcgtcgtact accgacgatc ggtgttggcc gctccgcgcg ggaatcagcg
     2221 gatgattgag atctcagacg ccatccgaga aggggcgatg gcattcctcc gtcgggagta
     2281 cacctggatt ctgatctttg ttgcggcgat gactgccctc attgcctttc tcctcgattg
     2341 ggggtggccg tggggcgccg ccgcctacgt gttcggtgcc gccctgtccg ccggtgccgg
     2401 ctacgccggg atgcgcatcg ccaccgcggc caattcccgg accaccgaag cggcgcgcac
     2461 gggaggagtt tcagccgcat tggcgattgc cttccgcggc ggtgcagttg ccgggttcac
     2521 catcgccggt ttcggcctac ttggtgtggg cctcgggttt ctcatcttcg atcgggtgct
     2581 cgaactcgat tcctgggcag agagcatcac cgctatcgcc ctcggtggct catcgatcgc
     2641 aatctttgcc cgggtcggag gtggcatctt caccaaggcg gccgatatcg gcgccgacct
     2701 cgtgggcaag gtcgaggctg gaatcccgga ggacgacccg cgcaaccctg ctgtcatcgc
     2761 tgacaacgtg ggcgacaacg taggtgacgt ggccgggatg ggtgccgacc tcttcgagtc
     2821 ctacgtcggc gcattggtgg ctccgatcgc ttacgcagcc atcgtgttcc gtggctcggg
     2881 cttcatcgag gagacgattc tctttcccct cgccatcgcc gccatcggga tgttgtccgc
     2941 aatcgtcggc tcgttcctgg tcagggccaa cgacgaaggc cacctctcgg ccgccctgca
     3001 ccgtggatcg aatttcgccc tggtcgtaac ggccctgggt gcgcttgggc tctcctactg
     3061 gatcttcggt gatgtgatcg agcatccgat cggggtctgg ctggcgataa tcgccgggct
     3121 tgtggtcggc tggctcgtcg gcaagatctc ggagtggttc acctcggaca accacaagac
     3181 ggtcaaagag atcgcccgtc aggcgcagac cggccctgcc acggtgatca tcaccggttt
     3241 ggccgaaggg atgcgctcag cggcgttctc tgtgattgtc gtggctacag gaatcggcgc
     3301 cgccttttgg gcaggtgact gggccatggg ggccggaggc ggcctatatg gagtggcgat
     3361 agccgcgatc ggtgtgctgg ccacccttgg catgaccatt tcgatggatg cttacggccc
     3421 gattgcggac aacgcaggtg gtatcgcgga gatggcccag ctggctcccg aagttcgtca
     3481 cgcaactgat tcgctggatg cgctgggcaa taccacagct gcggttgcca agggactggc
     3541 catcgcatcc gccgcggtca ccgctctcgc tctcttcacc gcattcatcg aggcggttgg
     3601 cctcaacgcg atcgacctaa cccgggcgga caccacgatc ggcttgctcc tcggagcgat
     3661 gttcccgttc ctgttcgcgg ccatgaccat gagcgcggtc gggcgaacgg cgaatcggat
     3721 gatcgatgag gtgcggcgtc agttccgtga gatcccgggg ctgcgagagg gtgaccctaa
     3781 agccagaccc gagtacgcca aatgtgtaga catcgcaacg acgggtgcgc tgcgcgagat
     3841 ggtgatcccg gcgaccatgg ccgtcgcatt gccgttggtc gtgggctttg tcgatatcga
     3901 ggcgctcggc ggcctcctcg gcggggcgtt ggtcacaggt ttcctgctcg ccatcttcat
     3961 gtccaatgcc ggcggggctt gggacaacgc caagaaattc atcgagaccg gtgcattcgg
     4021 gggcaaaggg tccgatgctc acaaggcggc agtcatcggg gacacagtcg gcgatccgtt
     4081 caaggacacg gcagggccct cgatgaacat cgtgatcaag gtgatggcca tcgtttcgct
     4141 gatctttgcc tcggccttca tcagttgaga tgccagaagt cctgctgatc cgccacgcag
     4201 agtccgagac caaccggtcc ggggtctggg caggccgctc ggatggtccc ctgagctcgg
     4261 ccggcgaggc cagtctcagg ccccttgctg agagattctc gaatcagaag ttcgagttgg
     4321 tgatgagctc cccattggag cgagcgacgc gcaccgcggc agccatctct ccccaggtcg
     4381 agatctccga ggaattcact gagatcgaca tcggacgttg ggaaggcagg acccgtgagg
     4441 aggtcctcgc ggccgacgat gacctgattc gcgaggcgag ggtgaatcgt gacctggctt
     4501 ggggtggcac cggggagagt ctccgtgacg ccgagcttcg ggcctggctc gctctcgacg
     4561 agctggcggg ccggctcggc gagcgggagc gagcagttgt ggtgacccat ggaggcctgc
     4621 tcaacgccgt tcttcatcgc tacctggccg gatcatccag gagggtgcat gcctttgccg
     4681 gcaacacttc tgtgacccgg ctggcctgga cccacgatcg accgagattg gcctccttca
     4741 acgaccaggg ccatctcggc cccaggtcac gggtcgtaga tgaggaattg gcggcagacc
     4801 gacccgtcct ggccctgatt cgccacggcc agacccaggc caacatcgaa ggtcgctggc
     4861 aaggccacgg tgactgggat ctcgacgagc ggggtcacac tcaggcggcg gctctacggg
     4921 actggtacgg aacgcacgac cttgtctatt cctcaccctt gagccgggca cagagcactg
     4981 cgggctacct ggccaacaat ggcgtggtga cggtcgaggc cctcaaggaa ctggggatgg
     5041 ggaagtggga ggggctcact tcgcccgagg tcatcgaaca atggccagat ctaatggatg
     5101 acatcttcag ggacggggtc gatctccgcc gtggtgaaaa tggggagagt tggggggagt
     5161 tggcgcatcg tgtccggaac gctgttcact ccctgcgccc tgcacgaggg gtcccgacgg
     5221 ttgccgtggc ccatggcggc gcgattcgct cttacatatc gtcattgacc gcgacgaccg
     5281 acactcatgc cgagtcgttg cacacgccca ggaacacgtc ggtgacccac ctctcctttc
     5341 acgacgatgg cccactgatc ctcgactacg cggttgcgac tcacctggaa accctctcct
     5401 aatctcgtct gatgtccgaa tacgactccc gtcgctacgc actggtcaat cggtgggatc
     5461 cccagcacct caagaccgtc gaccggctcc ttccgctcaa cccgggagat cgggtgttgg
     5521 aggtgggctg tgggagcggg cacctcacca agcgcctcgc cgcaagaggg atcgacgtcg
     5581 ttggcatcga cgccaatccc aacgctcggg aagtcgccgg caccgatcgt gtccttcaca
     5641 tgcgggccga acagctgggt ttcgaggata aggaattcga cgcagttatc tcggtgcatg
     5701 ccatcgagca catacctcca ctcgacgagg cagttgccga gatggcccgc gtgttgcagc
     5761 cgcagggtcg agccctgttg atctatccgg cggagccgat acaaggtctt tacgcgatcc
     5821 ccacgtcggt gatcctctat gggaccccgt ttaaagcccg cgaggtgcat tgccacaagc
     5881 tgtggccctc caaggtgcgg cgcatcgtgg aaccgttcgg gctcaccgag acccaccacg
     5941 agttcaacct gtggaagtcg ccgcagttcg tatccctctt cacccggttc taggggtgtt
     6001 tctctgtgac acctgcgggc gcacattcga agtccgtcaa gaggtcctcg accgctatcc
     6061 gggctgggtg ccccgtcagt gcctcgactg caagaacggc tcaggtggtg
//
LOCUS       DBNHXU010000317         2570 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_709504, whole genome shotgun sequence.
ACCESSION   DBNHXU010000317 DBNHXU010000000
VERSION     DBNHXU010000317.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2570)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2570)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2570
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_709504"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="AC161X_07900"
     CDS             complement(<1..576)
                     /locus_tag="AC161X_07900"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009776197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR4989511.1"
                     /translation="MARRPVVELAPGVWRIPLVGDWVNGFAFRDDDGQITLVDFGLKS
                     SATKVLAALETIGSGPSEVTRILLTHAHPDHAGGAAEISRNTGRAMGIHEADAEYLMA
                     GKSPPRDKSLLLGRLFNRMGGGGFEAVPVGERLTDGQVLPVNGGLRVVHTPGHSPGHV
                     AFLHEPTGVLIAGDSIFNVLGMRWSIASFCTD"
     gene            660..995
                     /locus_tag="AC161X_07905"
     CDS             660..995
                     /locus_tag="AC161X_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020542687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR4989512.1"
                     /translation="MVQYQVLDRTFSALADATRRDILDRLGRGPASISELAEPFGMTL
                     TGLKKHVSILEEAALVTTEKVGRTRLCSLGPERLEDAVSWIEMYRRHWERRIDGLEAY
                     LETKKGDKK"
     gene            992..1441
                     /locus_tag="AC161X_07910"
     CDS             992..1441
                     /locus_tag="AC161X_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018817643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4989513.1"
                     /translation="MSYELRVERLIAATPEEVFDAYTDPVAQQAWFTILDPEMIVENE
                     VDLRVGGKWVSVWGFSPDQMFRETNVFEVVDRPNRLVSTSSGSSPDGMTLETHVEITF
                     EEQNGKTLMTVIQSGFPDEETKDFFASMAWIGAFDRLEAYLGTRTVT"
     gene            complement(1450..2019)
                     /locus_tag="AC161X_07915"
     CDS             complement(1450..2019)
                     /locus_tag="AC161X_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989514.1"
                     /translation="MGVAFAVLLLLSAGMVSAPRSDVPLTEIGDFYNENASVVLIAQA
                     VGLAATAIFAFFISGLARLIGDARVKVVGLVVAAASVLTAVPLIVMSFQGSDPSPTMV
                     SLTDLTDAILFLAIAVLLGLLATNEIFPMWIRGLSLVGALLTLVRGIFGFFPIFSSLD
                     FIAPLSFVVMVVVLSGWAFRFSSDKPLAV"
     gene            complement(2073..>2570)
                     /locus_tag="AC161X_07920"
     CDS             complement(2073..>2570)
                     /locus_tag="AC161X_07920"
                     /inference="COORDINATES: protein motif:HMM:NF020091.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003852 - 2-isopropylmalate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - L-leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-isopropylmalate synthase regulatory
                     domain-containing protein"
                     /protein_id="HIR4989515.1"
                     /translation="YLLKTEQELEIPRRLQIEFTRVIQGITDTTGQEITAAEIWTEFR
                     RHYLAILKPFELLGFEIARDAEMENRSAITATIRYNDEENVIRSDGGGSIEAFVNALR
                     KDLGVSCRVLDYHEHSIGAGTDAQAVCYVEMEVGDRTLWGVGINTDTTTASLQAIISG
                     INRAS"
BASE COUNT          525 a          733 c          841 g          471 t
ORIGIN      
        1 gtcggtgcag aacgatgcaa tcgaccaccg catgccgaga acgttgaaga tcgagtcgcc
       61 ggcgatcaag accccggttg gctcgtggag aaaggcgacg tgaccggggg agtgcccagg
      121 agtgtggacc acccggagcc cgccgtttac gggcagcact tgaccgtccg tcagacgctc
      181 gccgacgggg accgcttcga agcccccgcc gcccatccga ttgaacaacc ttcccagcag
      241 cagtgacttg tcgcgtggcg gggacttgcc cgccatcaga tattcggcat ctgcctcgtg
      301 tatccccatt gcgcgacctg tgtttcgtga gatttccgct gcaccaccgg catggtcggg
      361 atgggcgtgg gtgagcagga ttctggtgac ctcgcttggc cctgagccga ttgtctccag
      421 tgcggcgagc acctttgttg ccgacgactt gagaccgaag tcgaccaggg tgatctggcc
      481 gtcgtcgtca cgaaacgcga acccgttcac ccaatcaccg accagcggga tccgccacac
      541 accaggcgcc agctccacta ccggccgtcg tgccatgacc tgagcctagg gatggaagac
      601 gggaggctct ggcgtgtcgg ggcttgacgt gccgtcggtc gtgacatata gtgaaccaaa
      661 tggttcagta tcaggttctg gatcggacgt tctcggctct tgccgatgcg acccgccgcg
      721 acatcctcga ccggctgggg cggggaccgg cctcgatcag tgagctggct gagccattcg
      781 ggatgacgct caccggccta aagaagcacg tgtcgatcct ggaggaggct gcacttgtga
      841 ctaccgagaa ggtcgggcgg acacggctgt gcagtctcgg ccccgagcgg ctggaggatg
      901 cggtgagctg gattgagatg taccggcgtc attgggaacg ccgaatcgat ggtctcgagg
      961 cctatctgga gaccaagaaa ggagacaaga aatgagttac gagctcagag tggaacggtt
     1021 gatcgcggcg acacccgaag aggtgttcga cgcctataca gatcctgtcg cgcagcaggc
     1081 ttggttcacg atcctcgacc ccgagatgat cgtcgagaac gaagtcgacc tgcgggtcgg
     1141 aggcaaatgg gtgagcgtct gggggttctc acccgatcag atgttccggg aaaccaacgt
     1201 gttcgaggtc gtcgatcggc cgaatcggct ggtctcgacg tcgagtggga gctcgccgga
     1261 tgggatgacc ctggaaaccc acgttgagat cacgttcgaa gagcaaaacg gcaagaccct
     1321 gatgacggtg atccagagtg gattccccga cgaagagacg aaggacttct tcgcatcgat
     1381 ggcctggatt ggggccttcg accggctcga ggcctacctg ggcacgagaa ccgtgacctg
     1441 aaacgtcggt cagacggcta acggcttgtc tgatgagaag cgaaatgccc agccggacag
     1501 cacaacaacc atgaccacga agctgagggg ggctatgaag tcgaggctgc tgaagatcgg
     1561 gaagaacccg aatattcctc taaccagggt gagcagcgct ccgaccagtg aaagcccgcg
     1621 gatccacatg ggaaagatct cgttggtggc gagcagaccg aggagaaccg cgatggctag
     1681 aaacaggatt gcgtccgtga gatcggtgag gctcaccatg gttgggctgg ggtcagatcc
     1741 ctggaaggac atgacgatca gcgggactgc ggtgaggacc gacgccgcgg ccaccaccag
     1801 tccgacgacc ttgactctgg cgtcgccaat cagcctggcc aagcccgaaa tgaaaaaggc
     1861 gaaaatcgcg gtggccgcga gaccaacagc ctgggcgatc aggaccacgg atgcgttctc
     1921 gttgtagaag tccccgatct cggtgagtgg gacgtcggag cggggggcgc taaccatgcc
     1981 ggctgacaac agcagcagga cggcgaatgc aacgcccaac cagccgacgc gcttgtgaat
     2041 cgaggcggcg atggtgatgg cttcctattc gatcagctgg cgcggttgat gccggagatg
     2101 atcgcctgca acgaggccgt cgtggtgtcg gtgttgatgc caacccccca aagggttcgg
     2161 tcgcccacct ccatctcgac gtaacacacg gcctgggcgt cggtcccggc cccgatcgag
     2221 tgctcgtggt agtcgaggac tcgacaactt acgccaaggt cttttctgag ggcattgacg
     2281 aaggcctcga tcgatccgcc tccatcgctg cggatgacgt tctcctcgtc gttgtatcga
     2341 atcgtcgcgg tgatcgcgct ccggttctcc atttcggcat cccgggcgat ctcgaatccg
     2401 agaagttcga agggcttgag gattgccaaa tagtggcgcc ggaactcggt ccagatctcg
     2461 gccgcggtga tctcctgtcc ggttgtgtcg gtgatgccct ggatgacccg ggtgaattcg
     2521 atctggagcc ggcgggggat ttccagctcc tgctcggtct tgagcagata
//
LOCUS       DBNHXU010000318         2295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2892647, whole genome shotgun sequence.
ACCESSION   DBNHXU010000318 DBNHXU010000000
VERSION     DBNHXU010000318.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2892647"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..523)
                     /locus_tag="AC161X_07925"
     CDS             complement(<1..523)
                     /locus_tag="AC161X_07925"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF020013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4989516.1"
                     /translation="MIHPGPLEADSSASWERLRCRVCASDDLVEVLSLGELPAANAYL
                     RPEDLDAPEPRFPLSLRLCEVCGMVQLGHVVPPDLLFRSYLFFTSSSVRMTEHFSKLM
                     NGMADAFTRPKGLVVEIGSNDGTALRSLAGRDLRILGVDPSRNLSEIATATGVPTIDE
                     FFTEDLATRIVETH"
     gene            complement(520..1248)
                     /locus_tag="AC161X_07930"
     CDS             complement(520..1248)
                     /locus_tag="AC161X_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011554415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CmcI family methyltransferase"
                     /protein_id="HIR4989517.1"
                     /translation="MSRRSRVRSVDEVAEGLSGLWDQETGEVVASAGLQKAIVDQVQR
                     LYYHSDPAIWKATTYRGITTWKCPLDLWVYQEIITEVQPGVVVETGTGFGGSALFLAD
                     LCETLGHGQVFTIDIADRAVEVEHPRLTKLIGSSADPAIRDLVRGQIPANEPVMVILD
                     SDHTADHVLAELRLWGDVVTPGSYLIVEDTNINGHPVLPSFGPGPAEAVTSFLPERPD
                     FTVDDSREKFLITWNPGGFLRRAP"
     gene            complement(1259..>2295)
                     /locus_tag="AC161X_07935"
     CDS             complement(1259..>2295)
                     /locus_tag="AC161X_07935"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4989518.1"
                     /translation="EEDRLRLAATPRVASPLLSIAIPSFNRPRALAELLTSIANEVAA
                     CPEGLVELCITDDASPDPETLEVALAFVEEHTFASLRIQPSNVGLERNVMAAAEPCRG
                     EYLMVVGNDDLLERGALGTILDDINGTDAPVLLYGKRRINLDGSPRADVAGSIPIELA
                     PGEAHLFPTLVEAAGEQGLLSTFGFAGPIVVKREPFIAVDPSPYLELTLYARACVLIE
                     AFAGSQVFYRNTTTIVHRTQSPVEKHAESLGRMEEEFMSGGRTRLSRYFGTTLAAAMQ
                     RLIDRGVIDPHTIALMPERMMSNRLFVDWIANNRSMDPTFDETLSKQVVDDADRFFAA
                     LEADLAHEVR"
BASE COUNT          457 a          654 c          751 g          433 t
ORIGIN      
        1 catgagtctc gacgattctc gtggccaggt cttctgtgaa gaactcgtcg atggtcggaa
       61 cgccggtggc ggtagcgatt tcggagaggt tgcgtgaggg gtcgactcca aggatgcgga
      121 ggtcgcgacc ggccaagctt ctcagtgctg ttccatcatt cgagccgatt tcaacgacga
      181 ggcccttggg tcgtgtgaac gcgtcggcca tcccgttcat cagcttcgag aaatgctcgg
      241 tcatccgcac cgagctcgag gtgaagaaca gataggaccg aaacaacagg tcgggaggaa
      301 cgacatgccc gagttgcacc attccgcaga cctcgcaaag gcgtagggag agggggaatc
      361 ggggctcagg cgcatcgagg tcttcaggtc ggaggtacgc attggccgca gggagctcgc
      421 cgagagatag aacctcgacc aaatcgtcgg aggcgcaaac ccgacatctc aaccgctccc
      481 acgatgcgct ggaatcggcc tccaaggggc cggggtggat catggggccc gtcgcaggaa
      541 tccgccgggg ttccaggtga tcaggaattt ctcacgagag tcgtcaacgg tgaagtcggg
      601 acgctcgggg aggaacgagg tgaccgcctc cgcagggcca ggcccgaatg agggcagtac
      661 cgggtggccg ttgatgttgg tgtcctcgac gatgaggtag gacccgggag tgaccacgtc
      721 tccccaaagc cgaagttcgg cgagaacatg gtcggcggtg tggtctgagt caagaatcac
      781 catcaccggc tcgttggcag gaatctgacc acgaacgagg tcacggatcg cggggtcggc
      841 ggaggagccg atgagtttgg ttaggcgtgg atgttcaacc tcgacggcgc ggtccgcgat
      901 gtcgatggtg aacacctgtc cgtgtccgag ggtctcgcag aggtcggcga ggaagagggc
      961 gcttccgccg aatccggttc cggtctcaac aacgacaccg ggttggacct cagtgatgat
     1021 ctcctgatac acccaaaggt cgagagggca tttccaggtt gtgatcccgc gataggtggt
     1081 cgctttccat atcgctgggt cggagtggta gtagaggcgc tggacttgat cgacgatggc
     1141 cttctgtagc cccgccgacg cgacaacttc acctgtctcc tgatcccaga gcccgctcag
     1201 gccctccgcc acctcgtcaa cagatcgaac acgagatcga cgcgacattg cagccacgtc
     1261 atctgacctc atgtgcgagg tcggcttcga gagcggcgaa aaagcggtct gcgtcatcaa
     1321 ccacctgctt cgaaagcgtc tcgtcgaagg ttggatccat gctccgattg ttagctatcc
     1381 agtcgacgaa caaacgattc gacatcattc gttctggcat gagggcaatc gtgtgagggt
     1441 cgatcacacc cctgtcgata aggcgttgca ttgccgcggc cagggtcgtc ccgaagtagc
     1501 gggataggcg ggtgcggccg ccactcatga actcttcctc catccgcccc aaggactcgg
     1561 cgtgcttttc caccggtgat tgggtgcgat gaacgatcgt cgtcgtgttc cgatagaaga
     1621 cttgcgagcc ggcaaacgcc tcgatgagga cgcatgccct ggcatacaag gtgagctcca
     1681 agtatggcga ggggtcaacc gctatgaatg gctccctttt caccacgatt gggcctgcaa
     1741 agccgaacgt cgagaggagg ccttgttcgc ctgcggcctc gacgagcgtg gggaagaggt
     1801 gggcttcgcc tggagccagt tcgatcggaa tcgagccggc cacgtcggcg cggggagacc
     1861 cgtcgagatt gatccggcgt ttcccataga gcaggacggg agcgtcagtt ccatttatgt
     1921 cgtcgagaat cgttccgagc gcaccccttt ccaacaggtc gtcattgccg acgaccatta
     1981 gatactcgcc ccggcagggc tcggcggcgg ccatcacatt gcgctccaga ccgacattcg
     2041 acggctggat ccgaagcgaa gcgaatgtgt gctcctccac gaacgcaagt gcgacttcca
     2101 gcgtttccgg gtctggggat gcgtcgtcgg tgatgcagag ctcgacgagc ccctccgggc
     2161 aagcggctac ctcgttggcg atgctcgtca ggagctcggc cagcgcccgc gggcggttga
     2221 aagacggtat ggcgatgctg agtaatggcg atgccacccg cggcgtcgcc gcgaggcgaa
     2281 ggcgatcttc ctcgg
//
LOCUS       DBNHXU010000319         2264 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4878622, whole genome shotgun sequence.
ACCESSION   DBNHXU010000319 DBNHXU010000000
VERSION     DBNHXU010000319.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4878622"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1123)
                     /locus_tag="AC161X_07940"
     CDS             complement(<1..1123)
                     /locus_tag="AC161X_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium/proton-translocating pyrophosphatase"
                     /protein_id="HIR4989519.1"
                     /translation="MPIVLAAEGGFQEFTLGSTEWAWLVFALATSILAIIVGFFLSRG
                     VLRADQGTEKMQEIAGAIQEGAMAYLKRQFRTIALILIPLVVVVFLTSTVVLKPDGSE
                     ALSLFESGSARTLAFIAGCVMSGLTGFIGMRLAVQGNVRTAAAARSGSLPAALQIAFR
                     TGGVAGMFTVGLGLLGATVIIMIFQNTSSAILIGFGFGGSLLALFMRVGGGIFTKAAD
                     VGADLVGKIEQGIPEDDARNPATIADNVGDNVGDCAGMAADLFESYEVTLVASIILGV
                     AAFASIGANPVLGLIFPVIVRAIGVLASIVGVFRVRATENDTSALAPINRGFLTAGIL
                     TVLGTAAVAYFYVGNDHGGEGWRVLGAVVAGLILAQVVSR"
     gene            complement(1307..1999)
                     /locus_tag="AC161X_07945"
     CDS             complement(1307..1999)
                     /locus_tag="AC161X_07945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989520.1"
                     /translation="MSDSRAFYARSGSAWDDLVTMLHLPYTAWHLSYVAIGAGLAAVL
                     DWKILAGTLLAFFFGLGIGAHALDEVHDRPLRTGLSDSTLWALGIGGLVAGGALGVIG
                     AFVISPVLLAWVALGVVLAAGYGLEWSPILHSDWGFSVAWGAFPVLVGYWAQTESLSI
                     TVALIAASTTVLSRVQRELSTPARKIRRTDDAVEVSGWTRDQLLSTWERPLRLMSMAM
                     PLLALGLLASHL"
     gene            complement(1996..>2264)
                     /locus_tag="AC161X_07950"
     CDS             complement(1996..>2264)
                     /locus_tag="AC161X_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4989521.1"
                     /translation="AVLEFGRPDGVWGPLWWLYTRIALPVAGSLVGSGWGRVGRFLGP
                     SIDEFWSRNTLEGLVQEWEAAGIRQVRWKRMSLGGGVVIWGVKV"
BASE COUNT          489 a          737 c          698 g          340 t
ORIGIN      
        1 gccggctcac cacttgggcc aggatcagac ccgcgacaac ggcacctagg accctccagc
       61 cctcgccgcc atgatcgttc cccacataga agtacgcgac ggcggcagtg ccgagcacgg
      121 taaggattcc ggcggtgaga aagccacggt tgatcggagc cagcgccgag gtgtcgttct
      181 cggtggcccg gacccggaat actccgacga tcgatgcgag gactccgatg gcgcgcacga
      241 tcaccgggaa gatcagaccc agaaccgggt tggcaccaat cgaggcaaag gcggccacgc
      301 cgaggatgat cgaagcaacc agggttacct cgtaactctc gaagaggtcc gccgccattc
      361 cggcgcagtc accgacattg tcaccgacgt tgtcggcgat ggtcgccgga ttccgggcgt
      421 cgtcctcggg gatcccctgc tcgatctttc cgaccaggtc ggcgccaacg tcggcggcct
      481 tggtgaaaat cccgcctcca acccgcatga agagggccag cagcgaaccg ccaaagccga
      541 atccaatcag gatcgcagat gaggtgttct ggaagatcat gatgatcacg gtcgcaccga
      601 gcaggccgag cccaacggtg aacattccgg cgacgccacc ggtgcggaac gcgatctgaa
      661 gagcggccgg aagagagccg ctgcgggcgg ctgccgcggt cctgacgttc ccctggacgg
      721 cgagcctcat cccgatgaat ccagtgagac cggacatgac acagccggca atgaaggcga
      781 gggttcgtgc gctccccgat tcgaacagcg aaagagcctc agagccgtcg ggcttgagga
      841 cgacggttga cgtgagaaag accaccacta ccagcgggat gaggatcagg gcgatcgtcc
      901 ggaactggcg cttcaggtag gccatggcgc cttcctggat cgctccggcg atttcttgca
      961 tcttctcggt gccctggtcg gcgcgcagga cgccccggga taggaagaag ccaacgatga
     1021 tcgccaggat cgaagtggcg agggcgaaca cgagccaggc ccactcggtg gagccgagtg
     1081 tgaactcctg gaagccaccc tcggcggcga ggacgatggg catcgtcgct tggtcctttc
     1141 agttgatgac gctgatcagt gaggtgaaac tatgacccaa ggccgcttcc ggcccttttc
     1201 ggtgaatccg gggacggcgt tgctcccaac ggggggcgaa tctacatgtt ctgattcgac
     1261 tctacgaaat tcacttcgcg gttgcactct tccaaatctg gctgtctcag agatggctgg
     1321 ccagcaaacc gagggccaga agaggcatgg ccattgacat caggcggagg ggtcgctccc
     1381 aggtggagag caattggtct cgagtccagc cggacacctc aaccgcatcg tcggtgcggc
     1441 gaatctttcg cgccggggtg ctcaactcgc gctgcactcg gctcaagaca gtcgtggaag
     1501 ccgcaatgag agcgacggtg atcgacaggg actcggtctg agcccagtat ccgacgagaa
     1561 ccggaaaggc gccccaggca acgctgaacc cccagtccga atgaagaatc ggagaccact
     1621 ccagcccgta accagcggct agcaccaccc cgagcgcaac ccaggccaac agcaccggtg
     1681 agatcacgaa cgcaccgatc accccgagcg cccctccagc aaccaggccg ccgattccga
     1741 gggcccacaa ggtggagtcg ctgagcccgg tgcgcagggg gcgatcatgc acctcatcga
     1801 gggcgtgggc gccaatgcca agaccgaaga agaaagcgag cagcgtcccg gcgaggatct
     1861 tccaatcgag cactgcggcc agtcctgctc cgatggccac ataggagagg tgccacgccg
     1921 tgtacggcag atggagcatc gtgaccaggt cgtcccacgc cgatccggag cgggcgtaaa
     1981 aggctcgtga gtcgctcaca ccttgacgcc ccagatgacc acgcccccac ccaacgacat
     2041 ccgcttccag cgaacctgac ggattccagc ggcctcccat tcttggacca ggccttccaa
     2101 agtgtttctg gaccagaact catcgatgct gggcccaagg aacctgccca ctcgtcccca
     2161 cccactaccg accaaggatc cggccacggg cagggcgatc ctcgtgtaca gccaccacaa
     2221 cgggccccag accccgtccg gccgtccgaa ctcgagcact gcga
//
LOCUS       DBNHXU010000320         4052 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3886259, whole genome shotgun sequence.
ACCESSION   DBNHXU010000320 DBNHXU010000000
VERSION     DBNHXU010000320.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4052)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4052)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4052
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3886259"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..974)
                     /locus_tag="AC161X_07955"
     CDS             complement(<1..974)
                     /locus_tag="AC161X_07955"
                     /EC_number="5.4.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012428.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HIR4989522.1"
                     /translation="MLAAEDRMSTILDDLIEKEERTFIERAPEATRLDTVARGSLAGG
                     VTSSWQISRPQPIWIDRGLGSRVWDVDGNEYVDFHGGYGAMLAGHANPAVVAAVSERV
                     RKGTHFAQPTEDSIVVAEELSSRYGQPLWRFGNSGTEATMDAVHLMRAVKGRDLIIKF
                     EGAYHGHHDSVMVSVWNEEDLGPEKRPASMPAGTGIPKAIVELALVATFNDLEVVSML
                     FDEHPGQIAGVIVEPILQNCGILLPEDGFLQGLRELTRQHDALLTFDEVKTGLTVGKG
                     GATRRFGVQPDIVCLAKALGGGLPISAIGGTDEVMGRIVDGTYDQVGT"
     gene            1081..2259
                     /locus_tag="AC161X_07960"
     CDS             1081..2259
                     /locus_tag="AC161X_07960"
                     /EC_number="1.14.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011727100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating oxygenase subunit
                     alpha"
                     /protein_id="HIR4989523.1"
                     /translation="MNVAPLDQAQVRKVIDDFGQNLTLPSAAYTSSEVLAWEKANLWQ
                     GTWVCVGRLEELVAPRQVRAVEMGDESVLLVRDEDGTLRAFSNVCRHRGHELAPVGDA
                     IDVRLIRCPYHAWSYRLDGSLRAAPTFTQSPTFDMGEFPLISIGVDVLGGWVFVDLSG
                     SAPPLASHFGNLAEIISDYEPERLVTAASHSYEVEANWKVIVENYNECYHCSNIHPEL
                     CEVTPPDSGYDHIPTGMWLGGTMDLKEHAVTMSLDGASHGVNFRKIEGEQARHIWYIT
                     VAPNLLLSLHPDYVMTHRLTPIDVDRTFIECSWLFPPEAFDLPNFDPSYATDFWDITN
                     REDWSACEGVQRGMRNRGYRPGPLSRWEGTVYQFLGVMARVYLGEGMVVPTVPQRELH
                     "
     gene            2276..2683
                     /locus_tag="AC161X_07965"
     CDS             2276..2683
                     /locus_tag="AC161X_07965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010571913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR4989524.1"
                     /translation="MAENKTQQTKASVAQFLNNIPDDGQRKDSKELAKLMASITGEKP
                     KMWGESIVGFGDLHYRSPSGREGDWFKVGFSPRKQNLTLYLALIDVARHDDLLSKLGK
                     HSTGKSCLYVKKLEDVDQEVLEELIETAFSSLS"
     gene            complement(2676..3848)
                     /locus_tag="AC161X_07970"
     CDS             complement(2676..3848)
                     /locus_tag="AC161X_07970"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016494583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003995 - acyl-CoA dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR4989525.1"
                     /translation="MSTFLTSAERLLQTRAREFIDEVIPYEVEAEMNRGELPPEVSKA
                     HHDKARSLGFVSINMPESMGGPGYGMLEQALIQEQSGRVTNALGWVVSTGPSWIADVV
                     TDYQMERWIRPTVDGDIHECYAITEEGAGSDVDGIEATARPEGDGYVLNGTKWHVTSA
                     NLAAYVFFQAKLTDGPNAGQHVLFFVDMDAPGVECLRVPEYSHTYGHHHWEMKFTDVT
                     VPKENLVGSEGDGMGFSHAWFRYERLMIAARCCGAAERLIDEATAFAEERQAYGSSIA
                     EYQAIQFMLADSLTELWAARLMTYETARGIDAGVDLKVQHAQCSMSKLYASEMANRVA
                     DRAVQIFGGRGFMRENVAERFYRELRVDRIWEGTSEIQRIISADQLRKRGTRSLVN"
BASE COUNT          782 a         1263 c         1201 g          806 t
ORIGIN      
        1 aaggttccca cctggtcgta ggtgccgtcc acgatgcgac ccatcacctc gtcggtcccg
       61 ccgatcgctg aaatcggtag cccgccgccc agcgccttcg ccaagcacac gatgtcgggt
      121 tgcaccccga acctcctggt ggctcctccc tttccgacgg tgagcccggt cttgacttcg
      181 tcgaaagtga gcaaggcgtc gtgttgtctg gtcagctccc tcaaaccctg taggaagccg
      241 tcctcaggca ggaggatgcc gcagttttga aggatcggct ccacgatcac cccggcgatt
      301 tggccaggat gctcatcgaa caacatcgag acgacttcga ggtcgttgaa tgtcgcgacc
      361 agggccagtt cgacgatcgc cttggggatg ccagtcccgg ccggcatgct ggccgggcgc
      421 ttctcggggc ccagatcctc ctcattccaa accgacacca tgacggagtc gtggtgtccg
      481 tgataggcgc cttcgaattt gatgatcagg tcgcggccct tgacggcgcg catcaggtgg
      541 accgcatcca tcgtggcttc ggtccctgag ttgccgaaac gccacagagg ttgcccgtaa
      601 cgactggaaa gctcctcggc aacgactatc gagtcctcgg tgggttgggc gaaatgggtc
      661 cccttgcgaa cccgctctga gaccgcggcg accactgccg gattggcatg acccgccaac
      721 atggcaccgt atccgccgtg aaagtcgacg tattcgttgc catcgacgtc ccagacccgt
      781 gatccgagac cacggtcgat ccagatgggt tgcgggcgtg aaatctgcca gctggaggtc
      841 actcccccgg ccaatgatcc ccttgccacc gtgtccaaac gcgtggcttc cggggcccgc
      901 tcgatgaatg tccgctcttc tttttcgatc agatcgtcca agattgtgga catccgatcc
      961 tcggcggcga gcatgagtca ccttcctttc gggtaacctg actcgctgtt caggatacac
     1021 tgaggcccgc aggcttgccc gtggtttctg ggtttaacgg gtcaggagaa ggggaaatcg
     1081 atgaatgtcg caccgctaga ccaggcacag gtccgtaaag tcatcgacga cttcggacag
     1141 aatctcactc ttccgtcggc cgcatatacc tcatctgaag tgctggcttg ggaaaaggcg
     1201 aacctatggc agggcacttg ggtgtgtgtg ggccggctcg aggaactggt ggcgccccgg
     1261 caggtgcgag ctgtcgaaat gggcgacgaa agcgttctcc tggtccgaga tgaagacggc
     1321 accctgcgcg cattttccaa cgtgtgtcgg caccgcggcc acgaattggc cccggttggc
     1381 gatgccatcg acgtgcggct gattcgctgc ccgtatcacg cctggtcgta tcgattggat
     1441 ggcagtctgc gggcagcacc caccttcacc caaagcccga ccttcgacat gggcgagttt
     1501 cctttgatct caatcggcgt cgacgttctc ggcggctggg tcttcgtaga tctctctggc
     1561 tctgcgccgc cattggccag ccatttcggc aatctcgccg agatcatctc ggactacgag
     1621 ccggagcgcc tggtgacagc cgcatcccat tcctacgagg tggaggccaa ttggaaggtc
     1681 atcgtcgaga actacaacga gtgctaccac tgctccaata tccacccaga gctgtgcgag
     1741 gtcactccgc cggatagcgg gtatgaccac atacccaccg ggatgtggct gggcgggacg
     1801 atggatctca aagagcatgc agtcacaatg tcactcgacg gtgcatctca cggtgtcaac
     1861 ttccgcaaga tcgaggggga gcaggcacgc cacatctggt acatcactgt ggcccccaac
     1921 ctgctgcttt cgctccatcc cgactacgtg atgacccatc ggttgactcc gatcgacgtg
     1981 gacaggacct tcatcgagtg ctcctggttg ttcccaccag aggcattcga cctgccgaac
     2041 ttcgaccctt cttatgccac cgacttctgg gacatcacca accgtgagga ctggtcggca
     2101 tgtgaaggag tgcaacgcgg catgcgaaac cgtggctacc gaccgggacc gctaagccgc
     2161 tgggagggga ccgtctatca gttccttggg gtcatggccc gcgtctactt gggtgagggc
     2221 atggtggtcc ccaccgtccc tcagcgtgag ctccactaac cattaggttt gcccgatggc
     2281 cgaaaacaag actcagcaga ccaaggccag cgtcgctcag ttcctgaaca acatccccga
     2341 tgatggccag cgcaaggact cgaaagagtt ggcgaagttg atggcgagca tcacgggcga
     2401 aaagccgaag atgtggggcg agagcatcgt tggattcggt gacctccact accgatcgcc
     2461 ctccggacgt gagggtgact ggttcaaggt gggtttctcg ccacgcaagc agaatctgac
     2521 cctttacctg gcgttgatcg acgtcgcgag gcacgacgat ttgctcagca agctgggcaa
     2581 gcacagcacc ggtaagtcat gcctctatgt caagaagctc gaggatgtcg accaggaggt
     2641 cctcgaggag ttgatcgaga cggcttttag ctctctcagt tgacgagaga gcgggttccg
     2701 cgcttgcgga gctggtcggc actgatgatc cgctggatct cggaggtgcc ctcccagatc
     2761 cgatcgaccc gcagctcgcg gtagaagcgc tcggcgacgt tctctctcat gaaaccgcgt
     2821 cctccgaaga tctgtaccgc acggtccgca acccggttgg ccatctccga tgcgtagagc
     2881 ttggacatcg agcactgagc gtgttgcact ttgagatcca ctcccgcgtc gatgccgcgg
     2941 gccgtttcgt aggtcatcag ccgggccgcc cacaactcgg ttagggagtc ggccagcatg
     3001 aattggatag cctgatactc ggcgatgctc gacccgtagg cctggcgctc ctcggcaaag
     3061 gcagtcgcct catcgatcaa ccgttcggcg gccccgcaac atcgggcggc gatcatcagc
     3121 cgctcgtagc ggaaccaggc gtgggagaag cccatcccgt ccccctctga tcccaccagg
     3181 ttctccttgg gaaccgtcac gtcggtgaac ttcatctccc agtggtgatg gccgtaggtg
     3241 tggctgtatt cgggcacccg gaggcattcc acccctgggg catccatgtc gacaaagaag
     3301 aggacatgtt gtcccgcgtt gggaccatcg gtcagcttgg cctgaaagaa gacgtaggcg
     3361 gcgagattgg ccgaagtcac atgccatttc gtcccgttga gcacgtagcc atcgccttca
     3421 ggacgcgccg tggcttctat gccgtcaacg tccgatcctg ccccttcctc ggtgatcgca
     3481 taacactcgt gtatgtcacc gtcgaccgtg ggccggatcc agcgctccat ctgatagtcg
     3541 gtgacgacgt cggcgatcca cgagggcccg gtggagacca cccagccgag ggcattggtg
     3601 actctgccgc tctgttcctg gatcagcgcc tgctccaaca tcccgtatcc gggcccgccc
     3661 atgctctcag gcatgttgat cgagacaaat ccaagggatc gggccttgtc atggtgcgcc
     3721 ttcgacacct cgggagggag ctcgccgcgg ttcatctcgg cctcgacctc gtacggaatc
     3781 acttcgtcga tgaactcacg agcgcgggtt tggagaagac gctccgcaga ggtgagaaag
     3841 gtcgacacga ccagccgaat ctagcagcgc agtcctgaac ggccgttcag aaaacccctc
     3901 gattcccggt cggaggcccc aggcgacggg ttatcctgcc ccaccgaccc gaggtagcaa
     3961 ttgatccggg agacgccctt ccacccgagg accagcgccg ccaaccaaac cggtttgtgg
     4021 agtcactggt cgggatacct ggcaaccgag aa
//
LOCUS       DBNHXU010000321         1591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2694493, whole genome shotgun sequence.
ACCESSION   DBNHXU010000321 DBNHXU010000000
VERSION     DBNHXU010000321.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2694493"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="AC161X_07975"
     CDS             complement(<1..595)
                     /locus_tag="AC161X_07975"
                     /inference="COORDINATES: protein motif:HMM:NF013126.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR4989526.1"
                     /translation="MSETHSGNVADPHLMLAFEGTDVPSWLSQRLTESPPSGVTLFRE
                     WNMDSPGQAAELTARLQEENADELPLLIAADQEGGQLLGITGSTPFAGNMALGATGDH
                     DLVFRVTQAMGREMRAVGVNVNYAPCADVASQAGNPSLGIRSFGDDPTTVATMTAASV
                     EGFISAGVLPTVKHFPGKGEAMVDPHYHLPLLDLDRER"
     gene            677..>1591
                     /locus_tag="AC161X_07980"
     CDS             677..>1591
                     /locus_tag="AC161X_07980"
                     /inference="COORDINATES: protein motif:HMM:NF012356.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HIR4989527.1"
                     /translation="MSRSPSRKWWQDAVFYQIYPLSFADSDGDGYGDLEGIISHLDYL
                     TDTLGVDAIWLSPFFRSPMDDWGYDVSDHTDVDPLFGDLSIAKRMIDEIHKRGMHVIV
                     DYLMNHTSIEHPWFTESRSSADNPKRDWYVWRDPRPDGSEPTNWISVFGGPSWTLDNA
                     TGQYYRHTFLPHQPDLNWRNPAVREAMYDVARFWLDLGVDGFRVDAAHQMMKDPRERD
                     NPPAPEDHTRPWKDMGDYDRYVHLYDFAHPDVHDAHREFRSVLDAYPHDPVSVGEIHI
                     FDLPEWAAYYGEHLDQIHMPFNFHLMVTP"
BASE COUNT          294 a          514 c          485 g          298 t
ORIGIN      
        1 gtcgctcccg atcgagatcg aggagtggca ggtggtagtg aggatcgacc atggcctcgc
       61 ccttgcccgg gaagtgcttc acggtcggca gcacgcccgc agagatgaag ccttccactg
      121 atgccgctgt catcgtcgcc accgtggtcg ggtcgtcgcc gaaggaccgg attcccaggg
      181 aggggttgcc ggcctggctg gcgacgtcgg cgcaaggggc gtagttgaca ttgacgccga
      241 cggcccgcat ctcgcgcccc attgcctggg tgacccggaa caccaggtcg tggtcgccag
      301 tggcgcctag tgccatgttt ccggcgaatg gtgtcgagcc ggtgattccg aggagctggc
      361 ctccttcctg atcggctgcg atcagcaggg gcagctcatc cgcattctcc tcctgcagcc
      421 tggcggtcag ctcggcagcc tgacccggag agtccatgtt ccactcccgg aacagagtga
      481 ctcctgaggg tggggactcg gtaaggcgct ggctgagcca tgaggggacg tcggttccct
      541 cgaatgccag catcaaatgt ggatcggcga cgtttccaga gtgggtctcg ctcatgctgg
      601 aagcgattga actacaccga ggtcggtctc gctacacctg gatagccggc cccgactggc
      661 tatgttctcg ctcggcatga gtcgatcccc ttcgaggaaa tggtggcagg acgccgtctt
      721 ctatcagatc tacccgctca gcttcgctga ctccgacggc gacggctacg gcgacctcga
      781 aggcatcatc tcccatctcg actacctgac ggacaccctc ggggtggatg cgatctggct
      841 gtcgccgttc ttcaggagcc cgatggacga ttgggggtac gacgtctcgg atcacaccga
      901 cgtggaccca ctgttcggtg acctgtccat cgccaagcgg atgatcgacg aaatccacaa
      961 gcggggaatg catgtgatcg tcgactacct catgaatcac acctccatcg agcatccctg
     1021 gttcacggaa tcgagaagct cggccgacaa cccgaaacgg gattggtatg tctggcgcga
     1081 ccccaggcct gatggatccg agcccaccaa ctggatcagc gtgttcggcg ggccgtcgtg
     1141 gaccttggac aacgccaccg gccagtacta ccggcacact ttccttcctc accagcccga
     1201 tctcaattgg agaaaccctg cggtccgcga agcgatgtac gacgtggcca ggttctggct
     1261 cgaccttgga gtcgacggat tccgggtcga cgccgcccat cagatgatga aagacccccg
     1321 ggagcgggac aacccaccgg cgcccgagga ccacacccgg ccctggaagg acatggggga
     1381 ctacgaccgg tacgtgcatc tctacgattt tgcccacccc gacgtccacg acgcccaccg
     1441 ggaattccgc tcggtcctcg acgcctatcc ccacgacccg gtgtccgtgg gagagatcca
     1501 catcttcgac ctgcccgagt gggcggcgta ttacggcgag catctcgacc agatccacat
     1561 gccgttcaac ttccatctga tggtgacgcc t
//
LOCUS       DBNHXU010000322         2486 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3092575, whole genome shotgun sequence.
ACCESSION   DBNHXU010000322 DBNHXU010000000
VERSION     DBNHXU010000322.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2486)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2486
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3092575"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..803)
                     /locus_tag="AC161X_07985"
     CDS             complement(<1..803)
                     /locus_tag="AC161X_07985"
                     /inference="COORDINATES: protein motif:HMM:NF018479.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low temperature requirement protein A"
                     /protein_id="HIR4989528.1"
                     /translation="MTGRLSFRRWWQPPGRYEDRPTDRRVTFLELFFDLVFVVLVSQL
                     AHHLAADPTWSGAAWFAFLFYAVWTSWINGTFYYDAHGTNDISVRVFTFAQMLTVALM
                     AVFAGDVPGSGAAGFAVAYGLNSLLLSIIWFRTGLHDPSHRSASNPYSAAYLLGGALF
                     IASAAVGEPVAYWMWGLALLIESLGYVLSTNRWQPPESQQGEATIATTPSLIERMGLF
                     VIIVLGEVIVDAIDGVADIHPLEVDGVIIGLLGVLVAIGLWWIYFDLVS"
     gene            complement(825..1850)
                     /locus_tag="AC161X_07990"
     CDS             complement(825..1850)
                     /locus_tag="AC161X_07990"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005248615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HIR4989529.1"
                     /translation="MELGITTFAETYPDPLTGEKISHGQRLRQVLDEIVLAEEVGLDV
                     YGVGEHHRPDFAASAPAVVLAAAAGMTKRIRLSPAVIILSSADPVRTFQDFATLDLVS
                     SGRAELLVGRGSFIESFPLFGYSLSDYDELFSEKLDLLLEIRDNEKVTWSGKHRPPLQ
                     DQRIFPRPEQDPLPVWVGVGGTPESIVRAATHGLPVALAIIGGNPDRFAFFADLHRRT
                     LVESGYDPAAVPLAVHAHGYIGDDADQAADEYYPSYAVAMSTIGRERGWGPMTRQQFD
                     AMRGPEGSLVLGDVEAVAAKIIRMREVLGIQRFMLHISVGTLPHDQVMRTIELLGTKV
                     APLVNAS"
     gene            complement(1897..2469)
                     /locus_tag="AC161X_07995"
     CDS             complement(1897..2469)
                     /locus_tag="AC161X_07995"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4989530.1"
                     /translation="MLIRSPRTISDDLRSETPHAARGKGPCLGAYGQPVASHPVRPVK
                     VSARIPVPPDLLFAFVSDTRNDPLWCDNVETVDQIEGDGVEAGARFRFHQHLDRPRSD
                     RIEFDADVVIIDVGPRTITWQVTDKFQDRNIQLIVEPDGAGSKITQITEASFQRPPGL
                     AKWAYPLLARRTFKGQFAQLAAYFEGLGAK"
BASE COUNT          518 a          745 c          801 g          422 t
ORIGIN      
        1 tgggatacga ggtcgaagta gatccaccac aacccgatcg cgaccaggac cccgagcagg
       61 ccgatgatca cgccgtcgac ttccaggggg tgaatgtcgg ccacgccgtc gattgcgtcg
      121 acgatgactt cgccgagaac gatgatcacg aaaagcccca tccgctcgat gagtgagggg
      181 gtggttgcga tcgttgcctc gccttgctga ctctccggag gctgccaccg gttggtggac
      241 agcacatacc ccaacgattc gatgaggagg gcgaggcccc acatccagta ggccaccggc
      301 tcaccaacgg cggcgctggc tatgaacaag gccccaccca gcagataagc cgctgagtag
      361 gggttcgacg ccgatcggtg cgacgggtcg tgaagaccgg tccgaaacca gatgatcgac
      421 agcagcaggc tgttgagccc gtaggcgact gcgaatccgg ccgccccgga gccggggacg
      481 tcgccggcga acaccgccat caaagccacc gtgagcatct gggcgaaggt gaacacccga
      541 acactgatgt cgttggtgcc gtgggcgtcg tagtagaagg tgccgttgat ccaagaggtc
      601 cacaccgcgt agaacaagaa ggcgaaccag gccgcaccgg accaggttgg atccgcggcc
      661 aggtggtggg ccaactggga caccaggaca acgaagacca gatcgaagaa gagctcgagg
      721 aaggtcaccc gtcgatctgt gggacggtcc tcgtagcgcc cggggggctg ccaccagcgg
      781 cggaaactga gtcgtccggt cacgaatccg aagctagccc ggacttacga agcgttgacc
      841 aggggcgcca ccttggtgcc cagcaactcg atggtccgca tcacctggtc gtgtggaagg
      901 gtgccgacgc tgatgtgaag catgaaccgc tggatcccga ggacttcgcg catccggatg
      961 atcttggcgg caaccgcttc gacgtcaccg agcaccagtg acccttcagg tccccgcatt
     1021 gcgtcgaatt gctgccgggt catcggcccc caccctcgtt cccgtccgat ggttgacatc
     1081 gcgacggcat aggaggggta gtactcatcg gcggcttggt cggcgtcatc gccgatgtaa
     1141 ccgtgggcgt gcaccgcaag agggaccgct gcggggtcat acccggactc gacgagggtt
     1201 cgccgatgga ggtcggcgaa gaaggcgaac cggtcagggt tgcccccgat aatggcgaga
     1261 gccacgggaa ggccgtgggt ggcggcgcgg acgatcgact ccggggtgcc tccgacaccc
     1321 acccacaccg gcagcggatc ctgctcgggc ctgggaaaga ttctctgatc ctggagcggg
     1381 gggcgatgct ttcccgacca ggtcactttc tcgttgtcgc ggatctccag gagcaggtcg
     1441 agcttctcac tgaacaactc gtcgtagtcg gacagcgaat acccgaacag tgggaaggac
     1501 tcgatgaagg accccctgcc taccagcagc tctgcccgac ccgaagagac aaggtccagg
     1561 gtggcaaagt cttgaaaggt ccggaccgga tcggcggaac tgaggatgat gacagcggga
     1621 ctgaggcgga tcctcttcgt cattcccgcc gcagcagcaa gaaccacagc aggggccgag
     1681 gcggcgaaat cagggcgatg gtgctccccg acgccgtaga catccagtcc aacctcctcc
     1741 gcaaggacga tctcgtcgag cacctggcgc agtctctgcc catggctgat cttctcgccg
     1801 gtgagcggat ccgggtaggt ctcggcgaaa gtggtgatcc caagttccat ggtgaggtct
     1861 gttcagcggt ggccggggtt gggatattcc cgatggctac tttgcgccga gcccttcgaa
     1921 gtatgccgcc agctgtgcga actgtccctt gaaggtgcgg cgagccagca acggataggc
     1981 ccacttggcc aagcctggcg gacgttggaa ggaggcctcg gtgatctgcg tgatcttcga
     2041 gcccgcgccg tccggttcca caatcaattg aatgtttcga tcctggaatt tgtcggtgac
     2101 ctgccaggtg atcgtcctcg ggccgacatc gatgatcacg acatcggcat cgaactcgat
     2161 tcgatcagat cgggggcgat cgagatgctg gtgaaaacga aatctcgccc cagcctcgac
     2221 accatcgccc tcgatttgat ccaccgtctc gacgttgtcg caccagagag ggtcgtttcg
     2281 cgtgtcggaa acgaaggcaa acaagaggtc cggtggcacc ggtattcgcg cggaaacctt
     2341 cacaggtcga acgggatgac tcgccacggg ctgaccatag gcgcccaaac atggaccttt
     2401 tcctctggcc gcatgaggag tctctgaacg aagatcgtcc gagatcgttc gaggagacct
     2461 gatgagcacc gcgcaaatcc aaatga
//
LOCUS       DBNHXU010000323         2465 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2894943, whole genome shotgun sequence.
ACCESSION   DBNHXU010000323 DBNHXU010000000
VERSION     DBNHXU010000323.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2894943"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..660)
                     /locus_tag="AC161X_08000"
     CDS             complement(<1..660)
                     /locus_tag="AC161X_08000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015798940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HIR4989531.1"
                     /translation="MNPTAEIYDTTLRDGTQAEGLSPTVGEKLRVVHLLDELGVHFIE
                     GGWPGANPKDDEFFQRAKSELSLERSILVAFGSTRRPREQVARDPQLQALLAADTDYV
                     CIVGKAWDRHVTGALNTSLEEGLDMVGESIEFLRGQGKQVFFDAEHFFDGFRSNPDYA
                     LSVLETAASAGAERVVLCDTNGGALPSHIIPALDAVKARIETKLGVHFHNDSDCAVAN
                     SM"
     gene            863..1768
                     /locus_tag="AC161X_08005"
     CDS             863..1768
                     /locus_tag="AC161X_08005"
                     /EC_number="2.7.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013775.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HIR4989532.1"
                     /translation="MDRYAPLPTPEQLERLSDDIGAPLTYDYRILGGLGGTMDVLRSE
                     VNRVVLKRYWLPEPDEEINPAESEYRALALAAEHGIPAPSPLWVDRVGLFPERAVVIS
                     FLEGKVILEPADPMDWATQLAMVLVAIHEMRPSPTDSIFPTLGHDDGHTSEKAVLEHP
                     LGADLWKKRSEAIATLIPDEVVYVHHDFWPGNTLWVDESLVAVVDWEGGSIADPALDV
                     ANCALDIRLLGNDDVADHFVAAYRNISGRALRNFRYWEINAVCRPMPDVAMWLPGWHA
                     VGLDVSPDQVRRRHWDLIEGVLGRG"
     gene            complement(1834..>2465)
                     /locus_tag="AC161X_08010"
     CDS             complement(1834..>2465)
                     /locus_tag="AC161X_08010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989533.1"
                     /translation="THRGASTVTDELRNRLSRLDPMASDVPTEPVTTESSQHLLEDIM
                     TTPNAEQPDSAGSPNKRPWLVAVAAAAVVIVAIGAVGLLTRDGGSDPIASGPPLELNA
                     GGEDPMAMCIMFSVEELAKAPVAFGGTATSVEGETITLDVDHWYKGGDAEQVVLHAPA
                     GMEALIAGIPFEEGGQYLITAYDGNVNYCGFSGESTPELQAAFEEAFPA"
BASE COUNT          435 a          790 c          773 g          467 t
ORIGIN      
        1 catggagttg gcgaccgcac agtcggagtc attgtggaag tggacgccga gcttggtctc
       61 gatccgcgcc ttcaccgcgt cgagggccgg gatgatgtga gagggcaggg cccctccgtt
      121 ggtgtcacaa agcacgaccc gctccgcacc cgccgaggcg gcggtctcca acaccgagag
      181 ggcgtaatcg ggattggagc ggaacccgtc gaagaaatgc tcggcgtcga agaagacctg
      241 cttcccctgt ccgcggagga actcgatcga ctcgccgacc atgtccaggc cctcctccag
      301 agaggtgttg agagcaccgg tcacatgacg gtcccaagcc ttgcccacga tgcacacgta
      361 gtcggtatca gccgcgagca gcgcctgaag ttgggggtcc ctcgccacct gttcccgagg
      421 tcgccgcgtc gatccaaaag caaccaggat cgaccgctct agtgagagct cgctcttagc
      481 cctctggaag aactcgtcgt cctttggatt cgccccgggc cagcccccct cgatgaaatg
      541 gaccccgagc tcgtcgagca ggtggacgac ccgcagcttc tcgcccaccg tcggagaaag
      601 gccctccgcc tgggtgccat cccgcagggt ggtgtcgtag atctcggctg tcggattcat
      661 ggtttcacac cttgccaaag aaaagacccc cgcaatctgc ggaggtccgt ggtggggcgg
      721 cgcctcatct gagacgcgaa cccctaggga atgatgatgt gcgttgttgc ctgggttctt
      781 cctcgcatcg aatagccagt atgggttggg tcggcgagat cgtcaagacg cattagggtc
      841 gccgcctctt cacctcgact ccatggaccg ttacgcacct ctccccaccc cggaacaact
      901 agagcgcctg agtgacgaca tcggtgctcc cctcacctat gactatcgaa tcctgggtgg
      961 gctcggcggc accatggacg ttctcaggtc cgaggtcaac cgtgtggtgc tcaagcgtta
     1021 ctggctcccg gaacctgatg aagagatcaa tccggccgaa tcggagtatc gcgctctggc
     1081 cctcgccgcc gagcacggaa tcccggcacc ttccccgctc tgggtcgaca gggtcgggct
     1141 gttcccggaa cgggcggtgg tcatctcatt cctcgagggc aaagtgatac tcgagccggc
     1201 cgacccgatg gactgggcca cccaactggc aatggtgctg gtggcgatcc atgagatgcg
     1261 cccatcgccg acagattcga tcttccccac cttgggccac gacgacggcc acacctcaga
     1321 gaaggcggtg ctcgagcatc ccctgggtgc ggacctgtgg aagaagagat ccgaagccat
     1381 cgccaccctg attccagacg aggtggttta cgtccaccac gatttctggc cgggcaacac
     1441 cctttgggtg gatgagagtt tggtggcggt cgtcgactgg gaagggggct ccatcgccga
     1501 tccggcgctc gacgtggcca actgcgccct cgacatcagg ctgctgggca acgacgacgt
     1561 ggcggatcac ttcgtcgccg cataccgcaa catctcaggg cgagccctga ggaacttcag
     1621 gtattgggag atcaacgccg tctgccgccc gatgccggat gtcgcgatgt ggttgcccgg
     1681 gtggcatgcc gtgggactcg acgtctcacc ggaccaggtt cgtcgtcgtc attgggatct
     1741 catcgaaggc gttctgggcc gcggctgagg tcactacgcg aaagaggagg gaccctcacc
     1801 gatccctcct cttcgtttgt ctgactcaga tgctcaggcg gggaaggctt cctcgaaggc
     1861 cgcctgcaac tccggggtgc tctcaccgct gaaaccgcag tagttcacat tgccgtcata
     1921 ggcggtgatc agatactggc cgccctcctc gaagggaatc ccagcgatca gagcttccat
     1981 cccggccggg gcgtggagca cgacctgctc ggcatcgccg cccttgtacc agtggtccac
     2041 gtccagggtg atggtctcgc cctccaccga ggtggccgtt cccccaaagg caacgggcgc
     2101 cttggccagt tcctcgacgc tgaacatgat gcacatcgcc atcgggtctt ctccgccggc
     2161 gttcaactcg agcggtgggc ctgaagcgat cgggtcgctg ccgccgtcac gagtgagcag
     2221 gcctaccgcg ccgatcgcca cgatgacgac agcggctgcc gccacggcga ccagccatgg
     2281 tctcttgttt gggctgcccg cggagtcggg ctgctcggca tttggggtgg tcatgatgtc
     2341 ctccagaagg tgttgggacg attcggtcgt cactggctcc gtcggcacat cggaagccat
     2401 cgggtcgagc cggctcaggc gattgcgcaa ttcgtcggtc acggtcgatg ctcctcggtg
     2461 tgtcg
//
LOCUS       DBNHXU010000324         1989 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3687574, whole genome shotgun sequence.
ACCESSION   DBNHXU010000324 DBNHXU010000000
VERSION     DBNHXU010000324.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1989)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1989)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1989
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3687574"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..827)
                     /locus_tag="AC161X_08015"
     CDS             complement(<1..827)
                     /locus_tag="AC161X_08015"
                     /inference="COORDINATES: protein motif:HMM:NF016946.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008488 - gamma-glutamyl carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0017187 - peptidyl-glutamic acid
                     carboxylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HTTM domain-containing protein"
                     /protein_id="HIR4989534.1"
                     /translation="MSPASAAVFRIGFGLGGLILVVRFLAKGWVETLFLEPAYHFPYP
                     GFEWVQVWPGWGMYAHFAMIGLAAVGILLGFRTRICALVFAVLLAYVELIDRTLYLNH
                     YYWVILTALVLAFLPMDRAYSVNARRHHGSESWIPSWVVWLLRFQVGMVYFFAGLAKL
                     NADWLIHGEPMATWLPGRSGLPLIGPMLALPATALVASWIGALFDLTIVFWLLNRRTR
                     LAAFIAVTMFHTATWILFPSIGLFPLLMTMSALIFFEPDWPERFAPLRVGAKPEREFS
                     "
     gene            complement(866..>1989)
                     /locus_tag="AC161X_08020"
     CDS             complement(866..>1989)
                     /locus_tag="AC161X_08020"
                     /inference="COORDINATES: protein motif:HMM:NF020932.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="imelysin family protein"
                     /protein_id="HIR4989535.1"
                     /translation="RFGAEIESHRSISPRQNDGQGRIQSWVRRLGAAVMALVLCACSG
                     GPTNADVVQSMANGALIPAMEAASVQAAALETAVSDFCFRPTVESQEAAHEAWRATKQ
                     AWQRSILTTWFGPAQMLRTVSHVDYEPIDEEGIDELLVSPEVLDVDYMTNQAAATQRG
                     LGSIEYLLFDNLQRAGDPRVCEMMMSTASVVASETFALETAWTDTYEDGSPFVERFLG
                     ETMTPEVAMANSISAIVETLKQMSLFQIGKALGISAQEPSIEAIPEGRAGFAGDAYVA
                     QLESIRSLLDGGGEASLGALIDSRSAEISDEIDDHLAAAIAELEGIDAPLRQVAADSP
                     GDLQSTYDHLSELLRIFEADVVSLLDITLGFSDADGDTG"
BASE COUNT          425 a          640 c          605 g          319 t
ORIGIN      
        1 gagaactcac gctctggttt ggcacccaca cgcaacggtg caaaacgctc cggccagtcg
       61 ggctcgaaga agatgagggc agacatcgtc atcagcagcg ggaacagccc gatcgagggg
      121 aacaggatcc aggttgcagt gtggaacatc gtcaccgcaa tgaatgcggc caaccgtgtg
      181 cggcgattca gcagccagaa cacgatggtc aggtcgaaga gggccccgat ccaggaggcg
      241 acgagggcgg ttgcgggtag agcgagcatc gggccgatca gcgggagccc cgaccttccg
      301 ggcagccagg tcgccatcgg ctctccgtgg atcagccaat ccgcgttcag tttggcgaga
      361 ccggcgaaga agtagaccat tcccacctgg aagcggagca gccacaccac ccaggacggg
      421 atccatgatt cggaaccgtg gtggcgcctg gcattcacgg agtacgcccg atccatgggt
      481 aggaatgcca gcaccagggc ggtgagaatc acccagtagt aatgattgag gtagagggtg
      541 cggtcgatca actccacata ggcgagcagt accgcgaaca ccagggcaca gatccgggtt
      601 cggaacccga gcaggatccc taccgccgcc agaccgatca tggcgaaatg ggcgtacatg
      661 ccccaacccg gccagacctg gacccactcg aagcccggat agggaaagtg atatgcgggc
      721 tcgagaaaca gggtctccac ccatccctta gccaggaagc ggacgacaag tatcaaaccc
      781 ccgagcccga atccgatgcg gaacacggcg gccgaagcgg ggctcaccgg ctgcctcgca
      841 cgctgagcga ccgccgccat ccggttcatc cggtgtcgcc gtcggcgtcg gagaacccga
      901 gggtgatgtc gagcagtgaa acgacgtcgg cctcgaagat ccgcaacagc tccgataggt
      961 ggtcgtaggt ggactgcagg tcgccaggac tgtccgcagc cacctggcga agcggagcat
     1021 cgatgccctc gagctcggca atggccgccg cgagatgatc atcgatctca tcggagatct
     1081 ccgccgaacg cgagtcgatg agcgccccca gcgaggcctc cccgccccca tcgagtaggg
     1141 agcgaatgct ttccagctgg gcgacataag cgtcgccagc gaacccagcc ctgccttcag
     1201 ggatggcttc gatcgatggc tcctgagccg agattccgag cgccttccca atctggaaga
     1261 gcgacatctg cttcaacgtc tcgacgatcg ccgaaatcga gttggccatt gccacctcgg
     1321 gcgtcatcgt ctccccgagg aatctctcca cgaacgggct tccgtcctcg taggtgtcgg
     1381 tccaggcggt ttccagggcg aaggtctcgg aagccactac tgacgccgtc gacatcatca
     1441 tctcgcagac ccggggatct cccgcccgct gcagattgtc aaagagcagg tactcgatag
     1501 aaccgagccc ccgttgtgtt gccgccgcct gattcgtcat gtagtccaca tcgagcacct
     1561 ccggtgaaac gaggagctcg tcgattccct cctcatcgat tggctcgtag tcgacatgag
     1621 aaacggtgcg cagcatctgc gccggtccaa accaggtggt cagaatcgag cgttgccagg
     1681 cctgtttggt agctctccat gcctcatggg ccgcctcctg ggactcaaca gtcggccgaa
     1741 agcagaagtc cgacaccgcg gtctccaggg cggcagcctg cacagaagcc gcctccatcg
     1801 ccgggatcag agcgccgttt gccatcgact gcaccacgtc ggcattcgtg gggccgccac
     1861 tgcaggcaca gagcaccagc gccatcacgg ccgctccgag gcgtctcacc cacgactgaa
     1921 ttcttccctg accatcgttt tggcgcggcg atatcgacct atgggactcg atctccgcgc
     1981 caaaacgga
//
LOCUS       DBNHXU010000325        14708 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1507857, whole genome shotgun sequence.
ACCESSION   DBNHXU010000325 DBNHXU010000000
VERSION     DBNHXU010000325.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 14708)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14708)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14708
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1507857"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..319)
                     /locus_tag="AC161X_08025"
     CDS             complement(<1..319)
                     /locus_tag="AC161X_08025"
                     /EC_number="1.16.3.2"
                     /inference="COORDINATES: protein motif:HMM:NF012436.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA];
                     GO_process: GO:0006879 - intracellular iron ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin"
                     /protein_id="HIR4989536.1"
                     /translation="MRMPEDLEAAFNRQVTMELTSATAYLQMAAFFASRNLVGMSSWM
                     RAQAGEERAHADRFFDFIVDRGNTVHLGDVPAPRSEFAGPEEAFEASLAQEQAVTEAI
                     HGLY"
     gene            complement(398..2800)
                     /locus_tag="AC161X_08030"
     CDS             complement(398..2800)
                     /locus_tag="AC161X_08030"
                     /inference="COORDINATES: protein motif:HMM:NF024910.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015171 - amino acid transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HIR4989537.1"
                     /translation="MSSRSVRSMWIKQRLFGRPLATDEEDHQLLPKVLALPVFASDPL
                     SSVAYATEEAMLVLALAGTAAFVYLTPVSLAVAVLLLIVIVSYRQTIRAYPDGGGAFI
                     VASENLGIRTGAVAAAALLTDYVLTVSVSVAAGVAAITSAAPNLIGWRVEMALGFVVL
                     LTVANLRGVREASRIFALPTYLFIVTVGVMLVVGFMKCMDGVCPTAVSSGLELQSEVG
                     VLGLFLILRAFASGSTALTGVEAVANGVQAFRPPKAANAGRTLAIMGVISITMFLGIS
                     ALALLFDVRISESTMDQYGTVISQIGRAAFNGGPLFYMLQVFTAAILILAANTAYQDF
                     PRLSAILARARLMPRQYLNRGDRLVFSNGVVTLAFIASVLLVLFQAEVSRLIQLYVIG
                     VFTSFTLSQTGMVRHWRKSREQGWRWSVVVNGVGAVTTGVVLVVVATVKFAHGAWIVM
                     LLIPLLVALMLGIRRHYLHVGAQLKHIRVDAQPRTTRVLVLVAHHDERTDRALQYVSF
                     LDVESVTCVHAEEERSDDIVYAWDVIHPKHPLEVLPGEHESISRRIVERVRKERNSHP
                     ESTVTVVLGEQVNTRSRFSTFSHRHSLAIKARLLFEPNVIVTDLNVLKRSRSSRLPRM
                     AISHVEQVVLISDMTRPIREALTYAQGLGAPTIAIHIDVDAGQRERVERHWKIAGYDF
                     PLEIVESPYRGIVDPLVDYLRERRRTALPGTLICAVLPEFVVPGRLTQLLHNQTGLAI
                     KGILAREVGIAVTSVPFHLEGNGKPHRESSEQPVSEGQAVPPAMQPAPAQNIDQIDPA
                     TP"
     gene            3083..3343
                     /locus_tag="AC161X_08035"
     CDS             3083..3343
                     /locus_tag="AC161X_08035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989538.1"
                     /translation="MSILFALSASGGVVTRFHLSPVPFVVGCGLLVLAAAATRRIAQR
                     AVQRPTNVVETGDLGREAALVGLVLAMLAAIALIAAGLFEYS"
     gene            3445..3579
                     /locus_tag="AC161X_08040"
     CDS             3445..3579
                     /locus_tag="AC161X_08040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989539.1"
                     /translation="MEDLLYVAAAVGFFVAAAGYVWFCDRLSTEDQPAHAVPSEEQPL
                     "
     gene            3600..3659
                     /gene="kdpF"
                     /locus_tag="AC161X_08045"
     CDS             3600..3659
                     /gene="kdpF"
                     /locus_tag="AC161X_08045"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR02115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA];
                     GO_process: GO:0043462 - regulation of ATP-dependent
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="K(+)-transporting ATPase subunit F"
                     /protein_id="HIR4989540.1"
                     /translation="MSIGLIVYLVVALLDPERF"
     gene            3659..5368
                     /gene="kdpA"
                     /locus_tag="AC161X_08050"
     CDS             3659..5368
                     /gene="kdpA"
                     /locus_tag="AC161X_08050"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017537411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpA"
                     /protein_id="HIR4989541.1"
                     /translation="MDSAAWLQLIALVALVALLIPVLGRYMAGIYGDGPGPLSRFFLP
                     LERGIYRLVGVDPEREQRWTGYALSLLAFSLVSTLGLYLLLRTQGWLPFNPTDMPNVP
                     PHLAFNIAVSFTTNTNWQNYSGEATLSHLTQFLGLVTQNFVSAAVGIGVMAALIRGLA
                     RRRTDTVGNFWVDLTRTTTRLLLPLCLLFAIVLVSQGVIQNFDGGVPAATAEGGSQLI
                     PGGPVASQLAIKQLGTNGGGFFNANSAHPFENPNGLTNFLQTVALVLIPFATVDTFGR
                     MIGNRRQGFILIVVMGVLWLGSTGVVVWAETSGNEALDGVGVDQVAAAESPGGNLEGK
                     EVRFGPAASAFWVASTTGSSNGSVNAMHDSLTPVGGLVALGNMVLGEVSPGGVGVGLT
                     GLLMFVVLTVFIAGLMVGRTPEFLGKKIQASEIKLATLYILAMPAVVLVLTAISVLLP
                     DALAARTNLGAHGLSEILYGFASPGNNNGSAFGGLSSNTDWYNPTQALAMLVGRFFLI
                     IPVLAIAGTLVRKPQLASSAGTFPTDRPLFAALLLGVVAIVAGLTFFPVLALGPIAEA
                     LSM"
     gene            5380..7413
                     /gene="kdpB"
                     /locus_tag="AC161X_08055"
     CDS             5380..7413
                     /gene="kdpB"
                     /locus_tag="AC161X_08055"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013570730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0031004 - potassium ion-transporting
                     ATPase complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpB"
                     /protein_id="HIR4989542.1"
                     /translation="MVRKPTKLFDPAIVRRAMKEALLKLDPRHLVRNPVMFVVGVGAL
                     FTSIIWMHDYLTSSSAPLGFTAQVIIWLWFTVLFANFAEAMAEGRGKAQAAALRSTRT
                     QTVANRRGRDGRIEVVPGASLDLDDVVVVSAGEVIPGDGEVVEGVASVDESAITGESA
                     PVIRESGGDRSAVTGGTKVISDEIAVRITARPGQTFIDRMIALVEGAERQKTPNEIAL
                     NILLAGLTIVFLLATVTLQPFAIYSGDKQEVVILVALLVTLIPTTIGALLSAIGIAGM
                     DRLVQANVLAMSGRAVEAAGDCSTLLLDKTGTITLGDRQARELLPASGVSDRELAEAA
                     QMASLADETPEGRSIVVLAKELYGIRAREMKGATLVPFSAHTRMSGVDYDGRRIRKGA
                     ASAVAAWVVDQGGVYPAEIETMVESVAGSGATPLVVAEGDRVLGVVALKDIVKDGIKE
                     RFEQLRQIGIRTVMITGDNPLTAQAIAAEAGVDDFLAEAKPEDKMALIRREQSGGRLV
                     AMTGDGTNDAPALAQADVGVAMNTGTQAAKEAGNMVDLDSNPTKLIEIVEIGKQLLIT
                     RGSLTTFSIANDVAKYFAIIPAMFAGVIPALGRLNLMRLETPRSAILSAVVFNALIIV
                     ALIPLALRGVRHRPGDAASILRRNVGIYGVGGLLIPFLGIKLIDLVVNALGLV"
     gene            7415..7981
                     /gene="kdpC"
                     /locus_tag="AC161X_08060"
     CDS             7415..7981
                     /gene="kdpC"
                     /locus_tag="AC161X_08060"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011802968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HIR4989543.1"
                     /translation="MRRQLAPAIRMFLLMTMLTGVAYTMVVTGIATVVFPEQAAGSLV
                     ESEDGVVGSRLIGQSFTSVDSFQPRPSAVDYDASGSGASNLGPTNPKLIEAVTGRVAY
                     YRTLNGLDASTPVPIDAVTSSASGLDPHISVANARLQASRVARERGLPLTTVMELVDR
                     HTVNPDLGFLSEPAVDVLALNLDLDSLG"
     gene            8024..10570
                     /locus_tag="AC161X_08065"
     CDS             8024..10570
                     /locus_tag="AC161X_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014055421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HIR4989544.1"
                     /translation="MNRGRLRVFMGAAPGVGKTFAMLEEAHRLRDLGHDVVIGFVETH
                     GRVDTAALIEGLEVVPRMELSHRGGQFEEMDLDAVIARRPTVALVDELAHTNVPGSRH
                     AKRWEDVEEILNSGIEVITTLNVQHLESLNDVIFEITGVQQRETIPDSVVRRADEIEL
                     VDLTQEGIRARMAAGKIYPAERVDAALANYFRPGNLSALRELALSWTADRVDEALAQY
                     RHAQGIEEPWETRERVVVGLTGALGGDAVVRRAARIAMRSRAELLGVYVRPTDGLSVA
                     EDEYLAEQRELLGQFGGSYHEIVGDDVGDSLLVFARSENATQIILGASRRSRLDEFLR
                     GSPIHKVIRRSGSIDVHVISYEGAPGAGYQPQKRAGRSALGVRRRVAAWSLLLAGLPL
                     LTWLLVLSGEMLGMQTNLLLYLLLAVGVGLIGGVLPAVVAAVAGFFLANWYLTPPVGT
                     LTIRSADDLVALIVFLLVAVSVGVLVGISTRQSADARRARAQAEALAATTSQPHPVLE
                     SGPAGSIDRIRDTFALRAVAVLRRGSEGWESLAQSGTPDLTNPDQGTEVIELTSDTVM
                     VLLDGKLSGDDRRVLRAFAVQIANALEREQLELEAGSAEAMAETDRLRTALLGAVSHD
                     LRTPLASIKASVTSLLETGVDWSPDQTEAFLVAILGETERLNRLVGRLLDASRVQMGA
                     LHVFFRPTVLAEVTASALAGLGPIQSRVEVDVPESLPEVQTDPELLERVIANLVDNAL
                     NWSPPDRAVRVTAGPVAGRIDLRVIDRGPGIPVADREKIFQPFQRLGDAPRGAGVGLG
                     LAVSRGFLDAMGNELLVEDTPGGGTTMVIAFKVVPTTRVATTIGTGEASG"
     gene            10563..11258
                     /locus_tag="AC161X_08070"
     CDS             10563..11258
                     /locus_tag="AC161X_08070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016432169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR4989545.1"
                     /translation="MAEPTRVLVVDDEPQIVRALAVNLQAIGYKVDVAHSGEEALKVA
                     ADHRPDAVILDLGLPGMDGLEVIQGLRGWTTVPIIVLSVRERDRDKIAALDAGADDYV
                     TKPFGMGELLARLRSALRRATAGDLDEPTLVTPDFTVDLSSKKVLRPDGEEVRLTPTE
                     WGIVEVLARHQGKLVSQTQLLKQVWGPDYGEETNYLRVFMAQIRRKLEPDLTHPRYFI
                     TEPGMGYRFEGNE"
     gene            11303..12259
                     /locus_tag="AC161X_08075"
     CDS             11303..12259
                     /locus_tag="AC161X_08075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014348316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4989546.1"
                     /translation="MTNAILAEGLRKEYGDVVALDGLDLAVPEGSVMCLLGPNGAGKT
                     TTVRILTTLLEATSGKATVAGLDVHADAARLRERIGLSGQYAAVDENLTGFENLEMVG
                     RLYHLGRGPSRERALDLLDRFDLDEAADRQAKTYSGGMRRRLDLAAALVARPEVIFLD
                     EPTTGLDPRSRLSLWEVIEELVKEGATLLLTTHFLDEADRLADSIAVIDHGKVIALGT
                     AEELKQQVGGERIELTVKSQADLVAAQEVLSSVGIGQVTVDEDARDLAVAVGVGGDAL
                     IETVRGLDARGISPLDLALRRPTLDDVFLSLTGHVAEEVVDA"
     gene            12256..13056
                     /locus_tag="AC161X_08080"
     CDS             12256..13056
                     /locus_tag="AC161X_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003945537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989547.1"
                     /translation="MSVMTALSDGAVVTKRNLLKITRLPDLLVFSTIQPIMFVLLFAY
                     VFGSAIVVPGVDYTDFLMAGIFVQTVVFGATITGAGLADDIKKGIIDRFRSLPMAQSA
                     VLIGRTTSDLLNNLLVVTIMALTGLLVGWRIHTSFVEALGGFALLLMFAYAVSWMMAS
                     VGLSVSSVEVVQNASFIVIFPLTFISNAFVPITNFPPVLKAFAEWNPVSAVTQAVREL
                     FGNVNPLAPPPDAWPLQNAILASMIWIVIFLVVFVPLAVSRYKKAAAR"
     gene            13359..13547
                     /locus_tag="AC161X_08085"
     CDS             13359..13547
                     /locus_tag="AC161X_08085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989548.1"
                     /translation="MFLANCDHLDREVIIWTSSLDSIENTENGILVRYQCACGRPAEM
                     LTGAASPIRVSVHVGAAA"
     gene            13612..14130
                     /locus_tag="AC161X_08090"
     CDS             13612..14130
                     /locus_tag="AC161X_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013673587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4989549.1"
                     /translation="MPINRLNHAVLYVRDADHSADFYTQKLGFRRLPLEFPGAVFLQA
                     EGSSNDHDLGLFSIGAGAAASPAGRGAVGLYHLAWEVDTLNDLARLAAVLSEADALVG
                     ATDHGSTKALYGRDPDGIEFEISWLVPADLLDEDSTPTARRLDLDAEIDRFGADTLGG
                     VGVSRPRIAVDS"
     gene            complement(14139..>14708)
                     /locus_tag="AC161X_08095"
     CDS             complement(14139..>14708)
                     /locus_tag="AC161X_08095"
                     /inference="COORDINATES: protein motif:HMM:NF019498.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4989550.1"
                     /translation="GISQRELARRLGLSPSLISQLESGLSKPSVGTLYAIVTELDLSL
                     DKLLRGADHSPSTSLAGRKAAVANGGPSPLVHPDDRESIDLASGVRWEQLSRDTDQGI
                     DFLHAVYEVGGASTPDESLMRHNGKEYGFIISGRLGIQLGFDTYELEPGDSIGFDSTQ
                     PHRLFNVGDEPVHAIWFVVGRTNDHRISP"
BASE COUNT         2603 a         4565 c         4653 g         2887 t
ORIGIN      
        1 ggtagagccc atgaatggcc tcggttacag cctgttcctg tgccagcgat gcctcgaagg
       61 cttcctccgg accggcgaac tcagatcgcg gtgcgggcac gtcgccgagg tgcaccgtgt
      121 tcccgcgatc gacgatgaag tcgaagaatc gatcggcatg agctcgttcc tcacccgcct
      181 gtgcccgcat ccagctgctc atgcccacca agttccggct ggcgaagaaa gctgccatct
      241 gcaggtatgc ggtggcagac gtcaactcca tggtgacctg tcgattgaat gcagcttcca
      301 agtcttcggg catgcgcatc tgagactcct cggttgggtc gtgcttccat cgtatcggcc
      361 ccatcgatgc ccaggatccg accctgaggc cgccctttta gggggtggcg ggatcgattt
      421 ggtcgatgtt ctgcgccggt gcgggctgca tggcaggcgg cacggcctgg ccttcgctga
      481 ccggctgctc agaagactcc cgatgaggct tgccattgcc ctcgagatgg aagggaacgc
      541 tcgtcacggc gattccgacc tctcgagcca ggatcccctt gatcgcgagc ccggtctgat
      601 tatgcagcag ttgggtcaac ctgcctggca ccacgaactc gggcagaaca gcgcagatca
      661 gagttccggg aagggcggtt cgccttcgct cccggaggta gtcgaccagc gggtcgacga
      721 tgccccggta cgggctttcc acgatctcca acgggaagtc gtagccggcg atcttccagt
      781 ggcgctctac ccgctcacgc tggccggcgt caacgtcgat gtggatggcg atggtgggcg
      841 cccccagtcc ttgggcgtag gtcagggctt cccggatcgg tcgggtcatg tcagagatca
      901 gcacgacctg ctcgacatgg ctgatcgcca tcctgggcag gcggctgctc cgagagcgct
      961 tcaggacgtt gaggtcggtc acgatgacat tgggctcgaa caggagccgc gccttgatgg
     1021 cgaggctgtg acggtggctg aaggtgctga atctggatcg ggtgttcacc tgctcgccga
     1081 ggaccacggt gacggttgac tcggggtggc tgttccgttc cttccgaacg cgctcaacga
     1141 tccgacgcga tatcgattcg tgctcgcccg gcaagacctc cagtggatgc ttggggtgga
     1201 ttacatccca cgcataaacg atgtcatcgg agcgctcctc ttcggcatgg acacaagtga
     1261 ccgattccac gtcgaggaac gagacgtact gcaaggcacg gtcggtccgc tcgtcgtgat
     1321 gggcgaccag aaccaggact cgggtggttc gcggctgggc gtcgactctg atgtgcttga
     1381 gctgcgcgcc gacgtggaga tagtgccggc ggatgccgag catcagggcg acgagcaatg
     1441 ggatgagcag catcacgatc caggccccgt gtgcgaactt cacggtcgca acgacgacga
     1501 ggaccacccc ggtggtcact gccccgacgc cgttcacgac aacggaccat cgccaaccct
     1561 gctccctgct cttccgccaa tgcctcacca ttcccgtttg gctcagggtg aaggaggtga
     1621 ataccccgat gacgtagagc tggatgagac ggctcacctc ggcctggaag agcaccagca
     1681 gcaccgaggc gatgaaagcg agcgtcacca caccattgga gaacacaaga cggtctccgc
     1741 gattcagata ttggcggggc atcagtctcg ccctagccag aatcgccgag agccgaggga
     1801 agtcttgata ggcggtgttt gccgccagga tcaggatcgc ggcggtgaac acctggagca
     1861 tgtagaagag aggcccgccg ttgaaggcgg ccctcccgat ctgtgagatc actgtcccgt
     1921 attggtccat cgtggactcg gagattcgga catcgaagag aagggccagc gcagagatcc
     1981 ccaggaacat ggtgatcgag atcactccca tgatggcgag agtcctgcct gcattggcag
     2041 ccttgggcgg acgaaacgcc tgaacaccat ttgccaccgc ttccacaccg gttagcgcgg
     2101 tcgaccctga ggcgaacgcc cggagaatca ggaacaagcc aagcactccg acttctgact
     2161 ggagctcgag gcctgaggag acggcagtcg ggcacacccc gtccatgcac ttcatgaagc
     2221 cgaccaccag catgacgccg acggtgacga taaaaagata ggtgggaagg gcaaagatcc
     2281 tcgatgcctc cctgacgcca cggaggttgg caaccgtcag cagcaccacg aatcccagag
     2341 ccatctcgac tctccaaccg atgaggttgg gtgctgccga ggtaatggcg gccactccag
     2401 ccgcgaccga caccgacacc gtcagtacgt agtcggtgag caacgccgcc gcagcgaccg
     2461 ccccggtgcg tatccccagg ttctcgctgg cgacaatgaa ggctccacct ccgtctggat
     2521 aggcccggat cgtctgtcgg taggagacga tgacaatcag caacaggact gccacggcca
     2581 gtgacaccgg cgtcaggtac acgaaggcag cggtgccggc cagagcgagg acgagcatcg
     2641 cctcctctgt ggcgtaggcc accgaggaca acgggtcgga ggcgaacacc ggtagcgcta
     2701 ggaccttggg cagaagctgg tggtcttctt cgtctgtggc caggggccgc ccgaacaacc
     2761 tttgcttgat ccacatgctt cgcacagaac gactgctcac cctccgcctc tctcttttgg
     2821 cgccaggcca tcgtgacgac tgcgcgagcc gtctagacgt gcagttgcgt tcctggattc
     2881 gaggccttaa aggtcaatga aacccctcac caggcattat cccgatcgcc ctaacgcgtt
     2941 cttaacgcgc cctatggcga tccttacggt gtcttgacct ccaaggcaag ggtcgtagcg
     3001 atggcgtaaa gatcaggccc cggcccgtca ggatcgtgtt catccccttc cggagggtgc
     3061 cccgacggat tacaccggcg gcatgagcat tcttttcgcc ttatccgcat cgggtggggt
     3121 cgtgaccagg ttccatctgt ccccagtgcc gtttgtggtc ggttgcggcc tgctggtcct
     3181 tgcagcggcg gcaacgcgcc ggattgcgca acgagcggtt cagaggccta cgaacgttgt
     3241 ggagaccgga gacctcggta gagaggccgc gctcgtcggc ctcgtacttg cgatgctggc
     3301 ggccattgcc ctgatagccg ccgggctttt cgaatacagc tgaccggcag atacccatca
     3361 gtaggtcgta ctgatatggg tgtcctccgc taacgacgcg ctaaggcgca ctgtctttcc
     3421 ttgtcttcgt cgtataccgg agatgtggaa gatctcttgt atgtcgcagc agcagtcggc
     3481 ttcttcgtcg cagctgccgg gtatgtgtgg ttctgcgatc ggctgagcac cgaggaccag
     3541 ccggctcatg cggtgccaag cgaggaacag cccctgtgat cgacctcatc gctctcattg
     3601 tgagcattgg gctgatcgtc tacctcgtcg tggctctcct cgatcccgag cgtttctgat
     3661 ggactccgcc gcctggcttc agttgatcgc cctggtcgcc cttgtggctc tcctcatccc
     3721 ggtgctaggg agatacatgg ccgggatcta tggggatggc cccgggccgc tgagtcgatt
     3781 cttccttccg ttagagcgcg gcatctatcg actggtcgga gtcgatcccg agcgagagca
     3841 gagatggacc ggctacgcgc tctcgcttct cgccttcagc ttggtttcca ccctcggtct
     3901 gtacctgctc ttgcggaccc agggctggct gccgttcaac ccgaccgata tgcccaacgt
     3961 cccccctcat ctggccttca acatcgccgt gagtttcacg accaacacca actggcagaa
     4021 ctattcgggc gaggcgaccc tctcgcatct cacacagttt ctcggtctgg tgacccaaaa
     4081 tttcgtatcg gccgcggtcg ggattggggt aatggcagcg ctcatcagag gcctggcccg
     4141 gcgtcggacg gacaccgtcg gaaacttctg ggtcgatctc accaggacca ccacccggct
     4201 cctgcttcct ctatgccttc tgtttgcgat cgtgctcgtc tctcaggggg tgattcagaa
     4261 cttcgacgga ggtgtgccgg ccgccaccgc cgaggggggc tcacagttga tacccggcgg
     4321 gccggtcgcc tcccagctgg ccatcaagca actcggaacc aatgggggcg gcttcttcaa
     4381 cgccaactcg gcccatccgt tcgaaaaccc gaacggcctc accaacttcc tgcagacggt
     4441 ggccttggtc ctaatccctt tcgcaactgt cgacaccttc ggccggatga tcgggaacag
     4501 acgtcagggt ttcatcctca tcgtggtcat gggggtgttg tggctgggat cgaccggtgt
     4561 ggtggtgtgg gcggagacct cggggaacga ggctcttgat ggagtcgggg tggatcaggt
     4621 tgcagccgcg gagtcgcccg gtggaaacct cgaaggcaag gaggtcagat tcgggcccgc
     4681 tgcctctgcc ttttgggtgg catcgacgac cgggtcctcc aacggctcgg tcaacgccat
     4741 gcatgattca ctgaccccgg ttggcgggct cgtggccctg ggcaacatgg ttctcggcga
     4801 ggtcagcccg ggtggggtag gggtcggcct caccgggctg ctgatgtttg tcgtgttgac
     4861 cgtgttcatt gccggtctga tggtcggccg aactcctgag ttcctcggga agaagatcca
     4921 ggcctccgag atcaaactgg cgacccttta catcctcgcc atgcccgcgg tggtcctggt
     4981 gctgaccgcg atctcggtcc tcctgcccga tgcgcttgcc gcccgcacga atctcggggc
     5041 acatggacta tccgagatcc tctacgggtt tgcctctccg ggcaataaca atggatcggc
     5101 gttcggcggg ctgtcgtcca acaccgattg gtacaacccg acgcaggctc tggccatgct
     5161 ggtcggccgg ttcttcttga tcatcccggt cctcgcgata gcgggcaccc tggtgcgaaa
     5221 gccgcagctg gcctcatcgg cagggacttt tccgaccgat cggcccctgt tcgctgcgct
     5281 actgctcgga gtcgtggcga ttgtggccgg cctcaccttc ttcccagttc tggcgctggg
     5341 cccgatcgcc gaagccctga gtatgtgagc agctgagaca tggtccgcaa gccaacgaaa
     5401 ttgttcgatc ccgcgattgt ccgccgggcc atgaaagagg ccttgctcaa gctcgacccc
     5461 cggcacctgg tgcgcaaccc ggtgatgttc gttgtgggag taggggctct cttcacttcg
     5521 atcatctgga tgcatgacta tctgacctcg agcagcgcgc ctctcgggtt caccgcccag
     5581 gtaatcatct ggctgtggtt caccgtcctc ttcgccaact ttgcggaggc gatggcagaa
     5641 ggccggggaa aggcgcaagc ggccgcgtta cgcagcacca ggactcagac cgttgccaat
     5701 cgccgcggtc gcgacggccg aatcgaggtc gtgccagggg ccagcctcga tctcgatgat
     5761 gtcgttgtgg tatctgccgg agaggtcatc cccggggacg gcgaggtggt cgagggcgtg
     5821 gcctcggtcg acgagtcggc gatcacgggt gagtcggcgc ccgtgatccg cgagtcaggt
     5881 ggagatcggt cggcggtcac cggtgggacg aaggtcattt ctgacgagat cgcggtgagg
     5941 atcaccgcac gccccggtca gacattcatc gacaggatga tcgcgctcgt ggagggggca
     6001 gaacgacaga agacgccaaa tgagatcgca ctcaacattc tcctggcagg gctgacgatc
     6061 gtgttccttc tggccaccgt cacccttcag ccgttcgcca tctactcggg cgacaagcaa
     6121 gaagtggtga tcctggtcgc gctgttggtc acattgatcc cgaccacgat cggtgccctt
     6181 ctctcagcaa tcgggatcgc aggcatggat cgactggtgc aggccaatgt cctggcaatg
     6241 tcgggccgcg ccgtcgaagc ggccggtgac tgctcgactc tgctcctcga caagaccgga
     6301 accatcacgc tcggtgaccg tcaggcgaga gagttgcttc ctgcgtccgg ggtcagcgac
     6361 cgcgagctcg ccgaggctgc acagatggcg agcctcgccg atgagacccc ggaggggcgg
     6421 tccatcgttg tccttgccaa ggagctctac gggatcaggg cccgggagat gaagggtgcg
     6481 accctggtgc cgttcagcgc ccatacccgg atgagtgggg tcgactacga cgggaggagg
     6541 atccgaaagg gcgccgcctc tgcagtggcc gcgtgggttg tcgatcaggg cggggtgtac
     6601 ccggctgaga tcgaaacaat ggtcgagtcg gtggcgggtt cgggtgcgac acccttggtg
     6661 gttgccgagg gcgaccgcgt gctcggcgtc gtggccctga aagacattgt caaggacggg
     6721 atcaaagagc ggttcgagca actccgacag atcgggatac ggacggtgat gatcacgggt
     6781 gacaacccgc tgaccgctca ggccatcgcg gccgaggcgg gcgtcgacga tttcctcgcc
     6841 gaagccaagc ccgaagacaa gatggctcta atacgccgcg aacaatcagg gggtcgattg
     6901 gtggcgatga ccggagacgg gaccaacgat gcccccgctc tggcgcaggc tgacgtcggg
     6961 gtggcgatga acaccggtac tcaagccgca aaggaggccg ggaacatggt cgatctcgac
     7021 tccaacccga cgaagctgat cgagatcgtc gagatcggca aacagctgct gatcacccgc
     7081 ggctccctca ccacgttctc gatcgccaac gacgttgcca agtacttcgc gatcattcct
     7141 gcgatgtttg ccggggtgat ccctgccctc ggccgtctca acttgatgcg ccttgaaacc
     7201 ccccgctcgg cgatcctttc tgccgtcgtc ttcaacgctc tgatcatcgt ggccctcatt
     7261 cctctggcct tgcgaggtgt ccgccatcgg ccaggggacg ctgcgtcgat ccttcgccgc
     7321 aacgtgggga tctatggggt cggcggcctc ctcattccat tcctcgggat caagctgatc
     7381 gatctcgtcg tcaacgcgct cggtctggtc tgagatgcga cgccaattgg ctcccgcgat
     7441 ccgaatgttc ttgttgatga ccatgctgac cggcgtcgcc tacaccatgg tggtgaccgg
     7501 gatcgctacg gtcgtgtttc cggaacaggc cgcgggctcc ctggttgaat cggaagacgg
     7561 tgtcgtgggc tcacggctaa tcggtcagag ctttacgagc gttgacagct ttcaaccccg
     7621 gccttccgcg gtcgactacg acgcgtcggg atccggcgca agcaacctgg ggcccaccaa
     7681 cccgaaattg atcgaagccg tgacggggcg ggtcgcctac taccggacgt tgaacgggct
     7741 cgacgcttca actccggtcc cgatcgatgc cgtcacctcc agcgcctcgg ggctcgatcc
     7801 tcacatctcg gtagccaacg cccgattgca ggcaagccgg gtggctcgag aacgcggtct
     7861 cccgttgacc acggtgatgg aacttgtcga tcgtcacact gtgaacccgg acctcggctt
     7921 tctcagcgag ccggcggtcg atgtcctggc tctgaatctc gacctggatt cgttgggatg
     7981 agcgcgtggg gaaataccgg gaaccggtac tagcttcaac gtggtgaatc gtggccggct
     8041 ccgtgtcttc atgggtgccg ccccaggtgt gggaaaaacg tttgccatgc tcgaggaggc
     8101 tcaccgtctg cgcgatctcg gccacgacgt agtgatcggg tttgtcgaga cccacgggcg
     8161 cgtcgacacg gccgcgctga tcgaaggtct cgaggtggtt ccgcggatgg agctgagcca
     8221 ccgcgggggg caatttgagg agatggacct cgacgcggtc attgcacgcc gcccaacggt
     8281 cgcactcgta gatgaattgg ctcatacgaa tgttccgggt tcccgccacg ccaaacgttg
     8341 ggaagacgtc gaagagatcc tcaacagcgg gatcgaagtc atcacgactc tgaacgtcca
     8401 gcatctggag tccctgaacg acgtgatctt cgagatcacc ggggtgcaac agagggagac
     8461 catcccggat tcggtggtgc gccgggccga tgagatcgag ttggtcgacc tcacccagga
     8521 ggggatcagg gcgcggatgg ccgccggcaa gatctacccg gcagaacggg tggacgccgc
     8581 cctggccaac tacttccgcc cggggaatct ctcggctctg cgcgagctag ccctttcctg
     8641 gacggcggat cgagtggatg aggcgctcgc tcaatatcgc catgcccaag gaatcgaaga
     8701 accttgggag acccgagagc gggtcgtggt tggcctgacc ggggcattgg gcggcgatgc
     8761 cgtggttcgg agagcggccc gaatcgcgat gcggtctcgc gccgagctgc tcggcgtcta
     8821 cgtcaggccc accgacgggc tctcggtggc cgaggacgag tacctcgcgg agcagaggga
     8881 gctgcttggc caatttggag ggagttatca cgagatcgtc ggcgacgatg tcggcgattc
     8941 gttgctcgtg ttcgccaggt cggagaacgc cactcagatc atcttgggtg cttcgcggcg
     9001 gtcccgactc gacgaattcc tccgcggttc cccgatccac aaggtgatcc gcaggtcggg
     9061 ctcgatcgac gttcacgtca tctcctatga aggcgctccc ggtgccggct accagcccca
     9121 aaagcgggct gggcggagtg ccctgggagt ccggaggcgg gtggcggctt ggtcgctgtt
     9181 gttggccggt cttccgctgt tgacctggct cctggttctt tccggcgaga tgttgggcat
     9241 gcagaccaac ctcttgctgt atctgctcct tgcggtcggg gtgggcctga tcggtggtgt
     9301 gctcccggcc gtcgtcgccg cagtcgccgg attcttcctc gccaactggt acctgactcc
     9361 gcccgtgggg accctcacaa ttcgctcggc cgacgacttg gtggccctga ttgtctttct
     9421 cctggtcgcg gtctccgtcg gggtgctcgt gggcatctcc acccggcaga gtgccgatgc
     9481 tcgccgtgcc cgagcccagg ccgaggccct ggcggcaacc acttcccaac cacaccctgt
     9541 gctcgaaagc gggccggccg gctcaatcga tcggattcgc gacacctttg cactgcgggc
     9601 ggtggccgtt ctgcgtcgcg gctccgaagg atgggagagc ctggctcaat ccggcacccc
     9661 cgacctgacc aatcccgacc agggaaccga ggtgatcgag ctcacctccg acactgtcat
     9721 ggtcctgctc gacgggaaac tgagcggtga tgaccgcagg gtgctgcggg ctttcgccgt
     9781 ccagatcgcc aatgccctcg agcgggagca actggagctc gaggctgggt cggccgaggc
     9841 aatggcggag accgatcgac ttcgcacggc tctccttggt gcggtttccc acgatctgcg
     9901 cacgcctctc gcctcgatca aggcatcggt caccagcctt ctcgagaccg gagtggattg
     9961 gtcaccggat cagaccgagg cattcctcgt tgcgatcctc ggcgagaccg agcgcctcaa
    10021 ccggctcgtt ggccgccttc tcgatgcaag tcgggtccag atgggtgcgt tgcatgtctt
    10081 ctttcggcca acggtgctgg cggaagtcac cgcctcggct ctggcaggcc tcgggcctat
    10141 tcaaagccgg gtggaggtcg acgtgcccga gtctctgcct gaagtgcaaa cggatcccga
    10201 gctgctggag agggtcatcg ccaacctcgt tgacaacgct ttgaactggt cgccgcctga
    10261 ccgcgcggtc cgggtcaccg ccgggccggt ggccggtcga atcgatctcc gcgtcatcga
    10321 tcggggcccg ggaatcccgg tggccgatcg ggagaagatc ttccagccgt tccaacgtct
    10381 cggtgatgcg ccgcggggtg ccggtgtcgg tctcggattg gcggtgtccc gtggattcct
    10441 ggacgcgatg ggcaacgagc tgctggtcga ggacaccccg ggcggcggga caacgatggt
    10501 catcgccttc aaggtcgtcc ccaccacgag ggtggcgacc actattggca ccggggaagc
    10561 cagtggctga gccgacccgg gtcctcgtcg tcgatgatga gccgcagatc gtccgcgccc
    10621 ttgccgtgaa tctccaagcg atcggctaca aggtcgacgt cgcccacagc ggcgaggagg
    10681 cgctcaaggt ggcagcggac catcgacccg acgcggtgat cctcgatctc ggtctcccgg
    10741 gcatggacgg gttggaggtc atccagggcc ttcgaggctg gaccaccgtg ccgatcatcg
    10801 tcctctctgt gcgggagagg gaccgagaca agatcgccgc cctggatgcc ggtgccgatg
    10861 actacgtgac caagccgttc gggatgggcg agctactcgc ccgattgcgc tcggccttga
    10921 ggcgggccac ggccggcgac ctggacgagc cgacgttggt cacccccgat ttcaccgtcg
    10981 acctctcatc caagaaggtt ctgcgacccg acggcgaaga ggtccgcctc accccgacgg
    11041 aatgggggat cgtcgaagtc ctggcaaggc atcagggaaa attggtcagt cagactcagt
    11101 tgctgaagca ggtctggggc cccgactacg gcgaagagac caactacctc cgcgtgttca
    11161 tggcccagat ccggaggaaa ttggagccgg acctcaccca ccctcgctat ttcatcaccg
    11221 agcccggtat gggctatcgg ttcgagggga acgaataacc gggatttgat gtcccgagca
    11281 ccggctactc tctcccgccc tcatgaccaa tgccatcctc gccgaaggtc ttcgcaagga
    11341 gtatggagac gtcgtcgccc tcgacggtct cgacctggcg gtgccagaag gatcagtcat
    11401 gtgcctgctc ggccccaacg gcgccggcaa gacaaccacg gttcgcattc tcaccacctt
    11461 gctcgaggca accagtggca aagccaccgt ggcggggctc gatgtccacg ctgatgccgc
    11521 caggctccgc gagcggatcg ggctctccgg tcaatacgcc gcggtcgacg agaatctgac
    11581 cggtttcgaa aatctggaga tggtgggacg gctctatcac ctgggtcggg gaccaagtcg
    11641 ggagcgcgcc ctcgacctgc ttgaccgttt cgacctcgac gaagcggccg accgtcaggc
    11701 caagacctat tcgggtggca tgcgtcgtcg tctcgacctg gctgcggcac tggtggctcg
    11761 tcccgaggtg atcttcctcg atgagccgac caccggcctc gatccccgaa gccggctcag
    11821 cctctgggag gtcatcgagg agttggtcaa ggagggcgcg actctgcttc tgaccaccca
    11881 tttcctggac gaagccgacc gactcgccga ttccatcgct gtcatcgatc acgggaaggt
    11941 gatcgccttg ggcaccgccg aggagttgaa gcagcaggtg ggcggggagc gcatcgagct
    12001 cactgtcaag agccaagccg atctggtcgc cgcccaggag gtgttgtcct cggttgggat
    12061 tggccaggtc acggtcgatg aggatgcgcg ggatctggcg gtggccgttg gggtcggcgg
    12121 cgacgcgctg attgagaccg ttcgcgggct cgacgcccgg gggatcagcc cgctcgatct
    12181 ggctctacgc cgccccacgc tggacgacgt ctttctgtca ctcactggcc acgtggctga
    12241 ggaggtggtg gacgcatgag tgtgatgact gccctctccg atggcgcggt tgtcaccaag
    12301 cgcaacctgc tcaaaatcac ccgtctcccc gacctcttgg tcttctcaac cattcagccg
    12361 atcatgttcg tgctgctctt cgcctacgtg ttcggcagcg cgatcgtggt gccgggtgtc
    12421 gactacaccg atttcctcat ggctgggatc ttcgtccaga ccgtggtatt tggtgccacc
    12481 atcaccggcg ccgggctcgc cgacgacatc aagaagggga tcatcgatcg atttcggtcc
    12541 cttcctatgg cccagtcagc ggtgctgatc ggccgcacca ccagtgacct gctcaacaat
    12601 ctcctggtgg tcaccatcat ggccttgacc ggcctgctgg ttggttggag gatccacacc
    12661 tccttcgtgg aggctctggg cggctttgcc ctactcctca tgtttgccta tgccgtctcg
    12721 tggatgatgg cgagcgtcgg actgtccgta agcagtgtgg aggtggtcca gaacgcctca
    12781 ttcatcgtca tcttcccgct gaccttcatt tccaacgcat tcgttcccat caccaacttc
    12841 cccccggtgc tgaaggcttt cgccgagtgg aacccggtct cggcggtgac ccaggcagtg
    12901 cgtgaattgt tcggcaacgt aaatcccctc gcgccgccgc ccgatgcttg gccattgcag
    12961 aacgcgatct tggcctcgat gatctggatc gtcatcttct tggttgtgtt cgtcccactt
    13021 gcggtgtctc gctacaagaa ggccgccgcc cgctgaccca tccctaatgc aacacggcgt
    13081 cgtatccggc gtctggtgca ttgggaagcc attcgacctg taatgaatca aataggtttg
    13141 actggttacc gtccaggcag taatgtaatg ctcgtaaaga gagatgctca ttacggaaca
    13201 ggagatggtg atgactccct acgctgcata tctgatcacc caggacatcc tggaccagcg
    13261 tcggcgcgaa ggcgataggg ctcgtctggt aagacaaggt gcccccaagc gaagcaagaa
    13321 gaccggccgc aaccgagcgt cttctcagga ggtgaccgat gtttctcgcc aactgtgacc
    13381 atctagaccg cgaggtcatc atctggactt cgagtctgga cagcatcgag aacaccgaaa
    13441 acgggatcct cgtgagatac caatgtgcgt gtggccgccc tgccgagatg ttgaccgggg
    13501 ctgcttcacc gatcagggtt tcggtccatg taggggcagc ggcctgaagc tgcccctaca
    13561 acggcactgt cactggggaa tagccctgct ctctaaacct ttgtaactgg catgccaatc
    13621 aatcgactca accacgcggt gctctatgtc cgcgacgccg accattcggc ggatttctac
    13681 acccagaagc tcggtttccg aagactccca ctcgaatttc ccggagcggt tttccttcag
    13741 gcagagggat cgagcaacga ccacgatctc ggactgtttt cgattggagc cggtgcagcc
    13801 gcctccccgg ccggacgggg cgctgtcggg ctctatcacc tcgcctggga agtggacaca
    13861 ttgaatgact tggcgcgtct tgccgcagtc ctttccgaag cggatgcact tgtgggtgcg
    13921 actgatcacg gcagcaccaa ggccttgtac gggcgcgacc ccgatggcat cgagttcgag
    13981 atcagttggc tggttccggc cgatctcctc gatgaggact ccacaccgac cgcccggcga
    14041 ctcgacctcg atgccgagat cgatcggttc ggggcagaca cccttggggg agtcggtgtg
    14101 agtcgcccac ggattgccgt cgactcctga gccagcccct agggcgagat cctatggtcg
    14161 ttggtgcgac ctaccacgaa ccagatcgcg tggaccggct cgtcaccgac attgaacagg
    14221 cggtgcggct gcgtcgagtc gaaaccgatg gagtcccctg gctcgagctc ataggtgtcg
    14281 aaaccgagtt ggatcccgag acggccggag atgatgaagc cgtactcctt cccgttgtgc
    14341 cgcatcagcg actcgtcggg tgtggatgct cctcccacct cgtacacagc atgcagaaag
    14401 tcgattccct gatccgtgtc gcggctgagt tgctcccagc gaactccgga ggcgaggtcg
    14461 atgctctcgc gatcgtcagg gtgaaccagc gggctcggcc cgccattggc gaccgcagcc
    14521 tttctccccg ccaaactggt cgaaggtgaa tggtcggccc cgcgcagcag cttgtccagt
    14581 gacaggtcga gctcggtgac tatcgcatag agggtcccca ccgacggctt ggaaaggccg
    14641 ctctcgagtt gcgagatcaa gctgggcgac agaccgagac ggcgggcgag ttcgcgctgg
    14701 ctgattcc
//
LOCUS       DBNHXU010000326         1525 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2301139, whole genome shotgun sequence.
ACCESSION   DBNHXU010000326 DBNHXU010000000
VERSION     DBNHXU010000326.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1525)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1525)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1525
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2301139"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="AC161X_08100"
     CDS             complement(<1..293)
                     /locus_tag="AC161X_08100"
                     /inference="COORDINATES: protein motif:HMM:NF014611.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004592 - pantoate-beta-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantoate--beta-alanine ligase"
                     /protein_id="HIR4989551.1"
                     /translation="MELTKTFDETRELYSGTVGLLPTLGFFHEGHLELMDMLRARCDT
                     LVVSHYVNPTQFNDPGDFEAYPRDVARDTDLASQHAVDIIFLPEQEEVYPEGWT"
     gene            complement(284..1093)
                     /locus_tag="AC161X_08105"
     CDS             complement(284..1093)
                     /locus_tag="AC161X_08105"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF022188.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Rossmann-like and DUF2520 domain-containing
                     protein"
                     /protein_id="HIR4989552.1"
                     /translation="MSGTGERMRLTIVGPGRAGGSIAIAAARAGHEICGVISRSGDAS
                     FGPLISADAPLPESDLLLISVRDEAIAQVAEELSSRAGSVAVAAHLSGFTPVTALRAL
                     AERGVSTGGFHPLQTLPDPERGADALAGSFVGLDGDALAMDTLTHLASSLGMEPFRLD
                     DTIRPSYHAAAAAASNFVLTALATSGDLFDSAGVDPRVARPLVERAVANFYEQGPQAL
                     TGPIARADTETVIGHLEAARTVSEEVGHQFRLMAEATAIRAGRSDEVDRWN"
     gene            complement(1126..1467)
                     /locus_tag="AC161X_08110"
     CDS             complement(1126..1467)
                     /locus_tag="AC161X_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989553.1"
                     /translation="MAKRSRVHRKEIPGYNESRPSSDDRKLEHRKVRHQTHQVLHMVD
                     DPDEVAELPDVRGTGEHDIFYVEAEPGKRRFRVWKTKFWKRRDDYHTMKSELDSNWPV
                     ITSEQLRQEQA"
BASE COUNT          265 a          490 c          477 g          293 t
ORIGIN      
        1 gtccaaccct ccgggtaaac ctcctcctgt tcgggcagga aaatgatgtc cactgcgtgt
       61 tgcgacgcca gatcagtgtc ccgggcaaca tcgcgcggat aggcctcgaa gtcaccggga
      121 tcgttgaatt gggtgggatt cacgtagtgg gagaccacca atgtgtcgca ccgggcacgc
      181 agcatgtcca tcaactcgag gtggccttca tgaaagaagc ccagggtggg aagcaagccc
      241 accgttcccg agtagagctc gcgggtctca tcgaaagtct tggtcagttc catcgatcga
      301 cctcgtcgga ccggccggcg cgaatggcag tggcctcggc catgagccgg aactgatggc
      361 ccacctcctc ggagacggtg cgagccgcct ccaggtgccc gatcacggtc tcggtgtcgg
      421 cccgagcgat ggggccggtc agcgcctgcg gtccctgctc gtagaagttc gccacggctc
      481 gttccaccaa cggacgggca actcgtgggt cgacgccagc cgaatcgaag agatcgcccg
      541 atgtggcaag cgccgtcaga acgaagttgg acgccgccgc cgccgctgcg tgatatgaag
      601 ggcggatggt gtcgtcgagt cggaatggct ccatccccag agaggatgcc agatgagtga
      661 gggtgtccat ggccagcgca tcgccgtcca agccgacaaa ggacccggcc agggcgtcag
      721 cccctcgctc gggatcgggg agagtttgca gcgggtgaaa cccgccggtg ctcacccccc
      781 tctccgccag agccctcaaa gccgtcaccg gagtgaatcc ggacaggtgt gctgccaccg
      841 cgaccgagcc agcccgactg ctcaactcct cggcgacctg ggctatggcc tcatcccgca
      901 ccgagatcag gagcaagtcc gattccggca gcggagcgtc ggcgctaatc agcgggccga
      961 agctggcatc gcctgatcgt gagatgacgc cgcagatctc atgtccggct cgggctgcag
     1021 cgatcgcgat cgacccgccg gccctccccg ggccaacgat cgtcagtcgc atcctctcgc
     1081 ccgtgcctga cacacgggca ggctagttgt gccctgcttc gagcttcagg cctgctcctg
     1141 gcgaagttgc tcggaagtga tcacgggcca gttcgagtcc agctccgact tcattgtgtg
     1201 gtagtcgtcg cgtctcttcc agaacttggt cttccacact ctgaagcggc gttttcctgg
     1261 ctccgcctcc acgtagaaga tgtcatgctc gccggtgcct cgaacatctg gcagctcggc
     1321 tacttcatcc ggatcgtcga ccatgtgcag cacctggtgg gtttgatgcc ttaccttgcg
     1381 gtgctccaac ttgcggtcgt cgctcgacgg cctgctctcg ttgtatccgg ggatttcctt
     1441 gcgatgcact cgcgaccgct ttgccatcta gatcgtttct ccttcctgga cggcgcatgt
     1501 cgatggttgg agaaagtcgg ggggg
//
LOCUS       DBNHXU010000327         4302 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3889614, whole genome shotgun sequence.
ACCESSION   DBNHXU010000327 DBNHXU010000000
VERSION     DBNHXU010000327.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4302)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4302)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4302
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3889614"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..910)
                     /locus_tag="AC161X_08115"
     CDS             complement(<1..910)
                     /locus_tag="AC161X_08115"
                     /inference="COORDINATES: protein motif:HMM:NF015662.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HIR4989554.1"
                     /translation="MAGPDAARRWNPAKVRIAVIGLTLVAAWGGMAYRLVQVQVVQAA
                     ELADKGLSQRLVSRELPPQRGKIYDRNGDLLAMTVETQSLYAVPGQVEDPVWVAQQVG
                     GVLGVDSDLLRDRLASDRDFVYLKRQVEADLADEVMGLEIKGVYSLPEPSRVYPAGQV
                     ASHVTGFVDIDGNGQEGLEYMYDDALRGLEGHALFERDLEGRPIPQGISEITPAVPGV
                     DLNTTIDLPLQYQAQQACLDAIEAPTAAKSCWVVVLSVETGEVLAMTGAPAFDPQTRE
                     NVDPRCVDDPDPVKCLTFSNFIVRGLY"
     gene            complement(995..1381)
                     /locus_tag="AC161X_08120"
     CDS             complement(995..1381)
                     /locus_tag="AC161X_08120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989555.1"
                     /translation="MTARPLRRVAETDPLRVLKGKRLSRPSLAPWMIMALIGVIAFLG
                     LGFARTSLDRSAFELSELTKSIDDATEINQSLSLEIARLENPARIAPLAEDLGLVIPS
                     NTKQLLVDLDPAGPRVADARVDGATQ"
     gene            complement(1378..2313)
                     /gene="rsmH"
                     /locus_tag="AC161X_08125"
     CDS             complement(1378..2313)
                     /gene="rsmH"
                     /locus_tag="AC161X_08125"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HIR4989556.1"
                     /translation="MSQDGNGNGRDYHRPVLAEEVVGLMAPALPGVVVDATFGGGGHT
                     RRLLDEFGDEVTMIGIDRDPAALANARVLGVVAIQGNFSELSALVSPIVPAQISAVLF
                     DFGVSSHQLDEPKRGFSYRNDGPLDMRMGPDAAHSAAEVVNEWPEARIAEVIRRYGEE
                     PLARRIAKAIVAARPIESTSQLSTVIAEALPAARRRAGHPARRTFQAIRIAVNEELEA
                     VADGLDQAIELVRPGGRVIAISYHSLEDRIVKRRFVKGATGCTCPPEMPVCGCGNTAE
                     LRILTRKAVRASADEIAGNNRARSAVLRAVEKVAA"
     gene            complement(2420..2833)
                     /locus_tag="AC161X_08130"
     CDS             complement(2420..2833)
                     /locus_tag="AC161X_08130"
                     /inference="COORDINATES: protein motif:HMM:NF014439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HIR4989557.1"
                     /translation="MFLGEFFHSLDGKGRVVLPSAFRRRLEAGCVVAKGQDGQLVIFA
                     VDDFEAKAAEVMEKPQNRGGRRFSRTVFGGADHQELDKSGRLLVKPDLRQFASLEGST
                     EIAVIGVFDHVELWNRDRYLTDRATGDESYLEEDD"
     gene            complement(3009..3680)
                     /locus_tag="AC161X_08135"
     CDS             complement(3009..3680)
                     /locus_tag="AC161X_08135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989558.1"
                     /translation="MSRLVKLLALLFALTACEGSVIFSGSASSPDPLVIPSTLSTPTT
                     IELTPAPGAEDYHWDAVTISDLNALRNGPVTFEVLDTLTNSCVDSVVAFTRMGQALLG
                     DVDRVLDVGVAAENGTGSIADASTAFDVWGNTALVVGYMAIHVVDNASFPPDAVGFID
                     QFGEAARKAGGYAFAIGNQAFIAADGEIDETKLEIWREHGLHDDALDELIAATDSVDS
                     EALCR"
     gene            complement(3731..4228)
                     /locus_tag="AC161X_08140"
     CDS             complement(3731..4228)
                     /locus_tag="AC161X_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR4989559.1"
                     /translation="MARVLVLNASYEPLSVVTTRRAVVLVLHQKASVVVGKDEVWLSE
                     HDSVEVPSVVRLNRFVKVPYRRAAPVTRRAVFGRDGHRCQYCGGPAESLDHIMPRSKG
                     GNHSWDNVVACCRPCNIRKGDRLPAEAGLRLDSKPGPPRQYAWIYASTGYQVDPAWHP
                     YLGAA"
BASE COUNT          778 a         1395 c         1351 g          778 t
ORIGIN      
        1 cgtagagccc ccgcacgatg aagttggaaa aggtgaggca tttcaccggg tccgggtcgt
       61 cgacacagcg ggggtcgacg ttctcccgag tctgtgggtc gaaggccggt gcaccggtca
      121 tcgccaagac ctcacctgtc tccaccgaga gcacgaccac ccagcaggac ttggcggcag
      181 tgggcgcctc gatcgcatcg agacaggcct gctgagcctg atactgaagg ggaaggtcga
      241 tcgttgtgtt gagatcgacc ccgggcacgg ctggtgttat ctcgctgatc ccctgcggga
      301 tcggccgacc ttccaggtcg cgctcgaaaa gagcgtgtcc ctccaacccc cgcagtgcat
      361 cgtcgtacat gtactccagg ccctcctggc cgtttccatc gatgtcgacg aagcccgtga
      421 catgactggc cacttgtccg gcgggataga cccggctcgg ctcgggcagg gagtagacac
      481 ctttgatctc cagccccatg acctcgtctg cgaggtcggc ctcgacctgg cgcttcagat
      541 agacgaaatc gcggtcggac gcgagccggt cgcggaggag atcagaatcc acaccgagga
      601 cacctcccac ctgctgagcc acccaaacag ggtcctcgac ttggcccggc acggcgtaga
      661 ggctctgggt ctccacggtc atcgccaaca ggtcaccgtt gcggtcgtag atcttccctc
      721 tctggggcgg gagctcacgg gacaccagac gctgcgaaag ccctttgtcg gcgagctccg
      781 ctgcctggac cacctggacc tggacgagcc ggtaggccat ccctccccag gcagccacga
      841 gggtcagccc gattacagcg atccgaacct tggccggatt ccaacgtcgg gctgcatcgg
      901 ggcccgccac gaagtcaggt ctccggccgg aaccccggtg gttcggagtg tcccactccg
      961 tccatcggcc cggctccagc gctgcgtcgg gtcatcattg cgttgctccg tcaaccctcg
     1021 cgtcggccac cctggggccg gccggatcga ggtcgaccag tagttgtttg gtgttggatg
     1081 ggatcacgag acctagatct tcggccagag gggcgatccg ggctgggttt tccagtcgag
     1141 cgatctcgag actcaggctc tgattgatct cggtggcatc gtcgatcgac ttggtgagct
     1201 cggacaactc gaacgccgag cgatcgaggg aggtgcgggc aaagcccaat ccgaggaagg
     1261 cgatcacacc gatcaaagcc atgatcatcc acggggccag tgacggcctg ctgagtcgct
     1321 tgcccttgag aacacgaagc ggatcggtct ccgcaacacg gcgcagcggt ctggcggtca
     1381 tgccgccacc ttctccactg cccgcagcac ggccgacctt gcccggttgt ttccggcaat
     1441 ctcgtcggcc gacgcacgga ctgccttgcg ggtgagaatc ctgagctctg ctgtgtttcc
     1501 gcagccgcac accggcattt ccggcggaca ggtgcaaccg gtggctccct tgacgaaacg
     1561 gcgcttgacg atgcggtcct ccaatgagtg atacgagatg gcgatgaccc ttcccccagg
     1621 tcggaccaac tcgatggcct gatcgaggcc atcagcgacg gcttccagct cctcattcac
     1681 cgcaattcgt attgcctgga aagttcgccg ggcgggatga cccgcccggc gacgggccgc
     1741 aggcagtgcc tctgcaatga cggtggatag ctggctggtc gactcaatgg ggcgggcggc
     1801 gacgatggcc ttggcgatgc gccgtgccaa cggctcctct ccgtatcgac gaatgacctc
     1861 ggcgatgcgg gcttcgggcc attcgttgac gacctcggcc gcactgtgtg cggcatcggg
     1921 ccccatccgc atgtcgaggg ggccatcatt tcggtaggag aacccgcgct tgggctcgtc
     1981 gagttggtgc gaggacactc cgaagtcgaa gagcacagcg ctgatctgag cagggacgat
     2041 cggggagacg agtgccgaga gctcactgaa attcccctgg atggcaacga ctccgaggac
     2101 cctggcgttg gccaaggccg cggggtccct gtcgatccca atcattgtca cttcatctcc
     2161 gaactcgtcc agcaagcgtc tggtgtggcc tccacccccg aacgtggcgt ccaccaccac
     2221 cccggggagc gcgggtgcca tcaacccgac gacttcctca gccaggaccg gccggtggta
     2281 gtcccggcca tttccgttcc cgtcctggct cattgccagc cccccgggcg ctggtaccaa
     2341 aggaagcggg cggagtcagg ggccttccaa ttggttccgg ggggctggcg atgggccagc
     2401 cagttcgatc ggtctcggtt cagtcgtcct cctccaggta gctctcgtcg ccggtggccc
     2461 ggtcggtgag ataccggtct cgattccaca actcgacgtg atcgaagact ccgatcaccg
     2521 cgatctcggt gctgccttcg agtgaggcga actggcggag gtcgggcttg accaggagcc
     2581 ggcccgactt gtcgagctct tgatggtcgg cgccaccgaa cacggttcgg gagaagcgac
     2641 gcccaccgcg gttctggggc ttctccatca cttcggcggc ctttgcctcg aagtcgtcga
     2701 cagcgaagat gaccaactgg ccgtcctgac ccttggcgac gacacagccc gcttcgagac
     2761 gacgacggaa agcggagggc aggaccaccc ggcccttccc atcgagactg tggaagaact
     2821 ctccaaggaa cacttcttct cgtttccgtc cgtccgcacc tgctgacccc ccttgtgggt
     2881 cgtgagcgcc actatgaccc actttccccc acttgtcaac cttttccccc acttggggtg
     2941 gtggctcccc cacaaagcgc gcgaaaaggt ggatgacgga tccaggaggt accggacgag
     3001 gccgatgcct atcggcagag agcctcgctg tcgaccgagt ctgtggcggc gatcagttcg
     3061 tcgagtgcgt cgtcgtgcag cccatgttca cgccagattt cgagctttgt ctcgtcgatt
     3121 tcaccgtcgg cagcaatgaa cgcctggttc ccgatggcga acgcatatcc cccggccttt
     3181 cgggccgcct cgccgaactg gtcgatgaag ccgactgcat ccggcgggaa tgacgcattg
     3241 tcgacaacgt ggatcgccat gtatcccacc accagggcag tgttgcccca gacgtcgaaa
     3301 gcggtggagg catcggcaat gctccccgta ccgttctcgg cggcgacacc gacgtcgagg
     3361 accctgtcca catcgccgag gagggcctgg cccatcctgg tgaatgcgac gaccgagtcg
     3421 acgcaggaat tggtgagagt gtccagcacc tcaaacgtga cagggccgtt ccgcaacgcg
     3481 ttcaggtccg agatggtgac tgcgtcccag tggtagtcct ctgcaccggg agccggtgtc
     3541 aattcgatcg tcgtgggtgt cgagagagtc gacgggatga ccaacgggtc cggcgagctc
     3601 gccgagccgg agaagatcac agacccttcg caggctgtca aggcgaacag gagggcaagg
     3661 agcttgacga gtctcgacac cacgctccct tcgtagacaa tgtctaccgg ccgggaccga
     3721 ccactcgact ctacgcggct ccgagatacg gatgccaggc cggatcgacc tgataaccgg
     3781 tcgaggcgta gatccaggcg tactgacggg gtgggcctgg cttgctgtcg agacggagcc
     3841 ccgcctcggc cgggagccgg tcgcccttcc tgatgttgca cgggcggcaa caggcaacga
     3901 cgttgtccca ggagtggttg ccacccttgg acctgggcat gatgtgatcc agggactcgg
     3961 cgggcccacc gcagtactga cagcgatggc catccctgcc gaacaccgct cgcctggtga
     4021 caggggccgc ccggcgatag gggaccttca caaagcggtt gagtcgaacc accgacggaa
     4081 cttcgaccga gtcgtgctcc gacagccaga cctcgtcctt gccgactacg acgctcgcct
     4141 tctggtgcag caccaagaca accgcccggc gcgtagtcac caccgaaaga ggctcataac
     4201 ttgcattgag gacaagaacc ctggccatag cgtctcttgc gacgcgacgt taccaagcag
     4261 tgctgggtgc gttcgtggca atggtctctt ccgacctgga aa
//
LOCUS       DBNHXU010000328         5257 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2697711, whole genome shotgun sequence.
ACCESSION   DBNHXU010000328 DBNHXU010000000
VERSION     DBNHXU010000328.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5257)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5257)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5257
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2697711"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1317
                     /locus_tag="AC161X_08145"
     CDS             <1..1317
                     /locus_tag="AC161X_08145"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF013736.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4989560.1"
                     /translation="TALVSRLFDQLSESIARYEKATRLQHAIATCSEALLVQTDVFAI
                     YEAVKALMEATDADYSYVARTIHVDGAPHWEIIADARRNANVRMGGWKSGEYDPTSAI
                     YKAQSEGRAITVHTARLKGEERAMYERDGIVSEVSVPIFVAGTFRGSIGFIATLEDRV
                     WSDQEVETLWRASHMIGAYWRRQDDQDELKASNESKDRLLASVSHEIRTPLTAIVGLA
                     EEIASSRSMLAADELVELNGIIAVQSRELAELVEDLLVASRAEAGNLSIKPDMIDLRA
                     EVESVVRGVRESHPSGKDLIVDGENVKAWADALRCRQILRNLLTNAVKYGGQRIAVLV
                     RSVGDQAQILVVDDGTGVPSEEQELIFERYYRGRQSPTQPGSVGIGLAVSRQLAELMA
                     GNLRYVDAHGESRFELTLPTHPSEPARSAPMSADPIMSSVDATFRV"
     gene            1424..2452
                     /locus_tag="AC161X_08150"
     CDS             1424..2452
                     /locus_tag="AC161X_08150"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HIR4989561.1"
                     /translation="MIAAVLLGAGALFAASTWGDVNRVTIDRPPVTVPVETPDNESDP
                     GQPDMNITVPPAGGDDLDVLLLVGSDSRDHLVDTEGFGDFGGHRADVVMVLIRPRDSD
                     RRAAVLSLPRDLLVADSCNGPEHRLNDALEGCGALMNGPTSLTVTVEDLIGLSVDHFA
                     LVDLAGFQEAVDAIGGYEICLQYAVRDEKAQLHLPAGCTLATGAQTLSWLRSRSTQEL
                     TAEGWKVMPGVSDLTRNQRQREFLLSMLGSLGDFSSPQDVASVAQTVAPYVTVDEDLS
                     LMNAVSLAWTMRGLGLGSVEELDFPVRSTTTSAGASVLLPSVDISEVVASYLAPELAQ
                     DSASGFAG"
     gene            2449..3450
                     /locus_tag="AC161X_08155"
     CDS             2449..3450
                     /locus_tag="AC161X_08155"
                     /inference="COORDINATES: protein motif:HMM:NF012401.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HIR4989562.1"
                     /translation="MSFPLPPGQIETKRFPVVGEREPSVDLGDRAGWTVEVAGLVERP
                     LQITLDAFLERANLPLTSDIHCVTSWSRLAMDWIGLPVRVLLDEAGLVDGAAYVSFEA
                     YSERAHHTSLPLQVVLESSWLMHTAGGQPLSVDHGGPVRVFTQGRYFYKSLKWVKRIV
                     VLAEDRPGWWETDSNYHNNADPLPGNERFTTGSIRPEQLRRFLTAPTYEKYRGRVMVG
                     LDLRSWSPKTKDLRRLYLKNCDLSGVDLSGVDMRESNLSLSDLRHAKLQGADLSGSDL
                     EGANFAGADLRDADVSGTALSATRFEEPGLGADVTGARFDGAWGLTETQEEYLAGRT"
     gene            3498..4478
                     /gene="trpS"
                     /locus_tag="AC161X_08160"
     CDS             3498..4478
                     /gene="trpS"
                     /locus_tag="AC161X_08160"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012643253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HIR4989563.1"
                     /translation="MTDRKRVFSGMQPTGEPHLGNLVGALRNWARMQDDYETIYCVVD
                     LHAMTVPYNPADLARERHDLARMILAAGIDPNRSLLYYQSEVPQHVELAWILGTITGI
                     GQLERMTQFKEKSDRSGQNLGILAYPVLQAADILIHKVHAVPVGDDQAQHLELTRDIA
                     ERFNARFGEEFPLPERITPEIGARVMSLTDPTAKMSKSDPSPRSRILLNDKPEDILKK
                     VKSAVTDTGSEVAYDWDEKPGISNLLELFSFFSGRTISDLADEYGPAGYGVFKAAVGE
                     SIVEGLRPIQEAYAGLSDSQVSQVMESSAAIARDRAETSMVTIREKAGLL"
     gene            complement(4482..5186)
                     /locus_tag="AC161X_08165"
     CDS             complement(4482..5186)
                     /locus_tag="AC161X_08165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0070007 - obsolete glutamic-type
                     endopeptidase activity [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HIR4989564.1"
                     /translation="MPKPWTVVDFIFVVLGGFLGAGVFAGLSLIWGDGDWFLVLGLAG
                     QYVGHLGVLWLLARRKPDSDLGFSIEPGDARYLALGLLLQLGLAVLFLPLTQFFFPEG
                     ESVQQVGSALASIESTAARVTAVVIAVVIAPVTEELIFRGVLLKSMGDRSRRAIIVVT
                     ALVFAAFHVIGLDPAHLLEASAVVLPQLFIVGAALAWVTLRTGRLGPAIFLHSGFNLL
                     AALVLLVPPEFLGVST"
BASE COUNT         1001 a         1606 c         1706 g          944 t
ORIGIN      
        1 acggctctcg tctcaaggct cttcgatcag ctttccgaat cgatagcgcg atacgagaag
       61 gcaacccgtc tccagcacgc catcgccacc tgctcggagg cccttctggt tcagaccgat
      121 gtctttgcga tctatgaggc tgtgaaggcg ctcatggagg cgaccgacgc cgactactca
      181 tatgtggccc gaaccattca tgtcgacggc gccccgcatt gggagataat cgccgacgcc
      241 cgtcgcaacg cgaacgtgcg gatgggcggc tggaagagcg gggagtacga cccgacgtcg
      301 gccatctata aagcgcagtc cgagggacgc gcgatcactg tccacactgc acgactcaag
      361 ggcgaggagc gggcgatgta cgaacgcgac ggcatcgtct cggaggtgag cgtcccgatc
      421 ttcgtggccg gcaccttccg cgggtccatc ggtttcatag cgaccctcga agatcgggta
      481 tggagcgacc aggaagtgga gacgctatgg cgcgcctccc acatgatcgg cgcctactgg
      541 cgtcgccagg acgatcagga cgagctgaaa gcttccaacg agtcgaagga ccggctcctg
      601 gcttcggtga gccacgagat tcggacaccg ctcacggcga tcgtgggtct ggccgaggaa
      661 atcgcaagca gccgatcgat gctggccgcc gacgagttgg ttgagctcaa cgggatcatc
      721 gcggtgcaaa gccgcgagtt ggctgagctg gtcgaggatc ttctggtggc gtcgcgtgcc
      781 gaggccggca acctctcgat caagcccgac atgatcgact tgcgagcgga ggtcgagagc
      841 gtggtccgcg gcgtgaggga gtcacatccc agcggcaagg acctgattgt cgatggtgaa
      901 aacgtcaagg cgtgggcgga tgcgctccgt tgccgccaga tcctccgcaa cctgctgacc
      961 aatgccgtca agtacggggg gcagaggatc gcggtcctgg tgcgttcggt cggcgaccag
     1021 gcccagatcc ttgtggtaga tgacggcacg ggggtgcctt ccgaggagca ggagctgata
     1081 ttcgagaggt actaccgcgg tcggcagtcg cccacccagc ccggatcagt cgggatcggc
     1141 cttgccgtct cccgccagct agccgagctg atggcaggca atttgcgata cgtggacgcc
     1201 catggtgagt cccgattcga gctgaccctg cccacccacc cctcggagcc ggctcgctcg
     1261 gcgccgatgt ctgcagaccc gatcatgtcg agtgtggacg caacgttccg agtttgattg
     1321 cctcctgaat cctcagctct gcgcgataca tagaatgtga tcccccaacc ggggctgacc
     1381 aagggcggac ctcctgaaga aacgattgtt gatagctggc gcgttgatcg ctgccgtcct
     1441 cctgggcgct ggagctctat tcgccgcgtc cacatggggg gacgtcaatc gagtcactat
     1501 cgaccgccca cccgtgacgg ttccggtcga gacaccggac aatgagtccg atccgggaca
     1561 gcccgatatg aacatcacgg tccctccggc gggcggggac gatctcgatg tcctccttct
     1621 cgtgggctcg gattcaagag accatctggt ggacacagag ggattcggtg acttcggagg
     1681 ccaccgcgcc gacgtagtca tggttctcat ccggccccgt gacagcgatc gccgcgccgc
     1741 cgttctctcg ttgccccggg acctcttggt ggctgacagc tgcaacggcc ccgaacatcg
     1801 gctcaacgat gcacttgaag gctgcggggc gctcatgaac ggcccaactt cgctcacggt
     1861 cacggtcgag gatctaatcg gtctgagtgt ggatcacttc gcacttgtcg atctggccgg
     1921 gtttcaggag gcggtggacg cgatcggggg ctatgagatc tgcctgcagt acgccgtccg
     1981 tgacgagaag gcccaattgc atctgccggc cggatgcact ctggcaacag gagcccagac
     2041 cctgtcctgg cttcggtcgc gcagcaccca ggagctcacc gccgaaggtt ggaaggtgat
     2101 gccgggagtc agcgacctga cccgtaatca gcgacaacgg gagttcctgt tgtcgatgct
     2161 cggcagtctc ggcgacttca gcagccctca ggacgttgcc tcggttgccc aaaccgtcgc
     2221 cccatacgta accgttgacg aagacctcag cctgatgaat gccgtgagcc tggcctggac
     2281 gatgcggggg cttggtctag ggtcagtcga ggagctggac tttcccgtcc ggagcacgac
     2341 tacctcagcc ggcgcctcgg tccttcttcc gtcggttgac atctccgagg tggtcgcctc
     2401 atatctggcc cctgagttgg cacaggattc ggcctccggc ttcgccggat gagctttccc
     2461 ctgccgccag ggcagatcga gaccaagaga ttcccggtgg tcggggaacg ggagccctcc
     2521 gtcgacttgg gcgatcgggc tggatggacg gtcgaagtgg ccggtctcgt cgagcgccca
     2581 ctgcagatca ccctcgatgc gtttctcgag cgggccaatc tcccgctgac ctccgatatc
     2641 cattgtgtga cgagttggag tcgccttgcc atggattgga tcggccttcc agtgcgggtc
     2701 ttgcttgatg aggccggact tgtcgatggc gcggcctacg tgtctttcga ggcctactcg
     2761 gagcgagctc atcacacctc tctcccgctc caggtggttc tcgaatcgag ctggttgatg
     2821 cacaccgccg gcggacagcc gctgagtgtc gaccatggcg gcccggtgcg ggtgttcacc
     2881 cagggtcgct acttctacaa gtcgctgaaa tgggtgaagc ggatcgtggt cctcgccgag
     2941 gatcggcctg gatggtggga gaccgattcc aactatcaca acaacgccga cccgcttccc
     3001 gggaacgaga ggttcactac cggatcgatc cgtcccgagc agctccgccg attcctgact
     3061 gctccgacct atgagaagta tcgcggccga gtcatggtcg gcctcgacct gaggtcgtgg
     3121 tcacccaaga ccaaggacct cagacgtctc tacctgaaga attgcgactt gtcgggcgtc
     3181 gatctttccg gtgtcgacat gagggagtcg aatctgtcgc tcagcgatct tcggcacgcc
     3241 aagctgcagg gcgccgacct ttcagggtcg gatctggaag gcgcaaactt cgccggcgcc
     3301 gatctgagag atgccgatgt ctcggggacc gccctttctg cgacccgttt cgaagaacca
     3361 ggccttgggg ccgacgtgac cggtgcccgt tttgacggcg cttggggatt gaccgagacc
     3421 caagaggaat acctggcagg ccgtacctaa ggtgtcgccc gacgccgtgt gattgtgtct
     3481 gtgcaggaaa ggcaaatgtg accgatcgaa aaagggtgtt ctcggggatg cagccgaccg
     3541 gcgagcctca cctggggaac ctggtggggg ccctgcgcaa ctgggccagg atgcaggacg
     3601 actacgagac catctactgc gtggtggatc tgcacgcgat gacggttccc tacaacccgg
     3661 ccgacctcgc cagggagcgg cacgatctgg cccgcatgat ccttgccgct gggatcgacc
     3721 ccaatcgctc gctgctctac taccagtccg aggttcccca acacgtagag ctggcatgga
     3781 tcctgggcac gatcacgggc atcgggcagt tggagaggat gacccagttc aaggagaagt
     3841 cggaccggtc cggccagaac ctggggatcc tcgcctatcc ggtcttgcag gccgcagata
     3901 tcctgattca caaggtccac gcggtcccgg tgggcgacga ccaggcgcag cacctcgagc
     3961 tgacccgtga catcgctgag cgattcaacg cccgcttcgg ggaggagttt cccctacccg
     4021 agcggatcac tcccgagatc ggagccaggg tgatgtcgct gactgatccg acggccaaga
     4081 tgtccaagtc cgacccgtcc cctcggtcca ggatcctcct caacgacaag ccagaagaca
     4141 tcctgaagaa ggtcaagtcc gcggtgactg ataccggttc ggaagttgcg tatgactggg
     4201 atgagaagcc gggtatctcc aatctcctcg agctgttcag cttcttcagt gggcggacga
     4261 tctccgacct cgccgatgag tacggccctg ccgggtatgg cgtattcaaa gcggcggtgg
     4321 gggagtcgat cgtcgagggt ctgcggccta tccaggaggc ctacgccggg ctcagtgatt
     4381 cgcaggtcag ccaggtgatg gagagcagcg ctgctatcgc ccgggatcgc gcggaaacgt
     4441 cgatggtgac gatccgggag aaggccggtc tgctctgaat gtcaggtgct gacgccgagg
     4501 aactccggcg gcaccaagag gacaagcgcg gcgagcaggt tgaaaccaga gtggaggaag
     4561 atcgccggtc cgagacgacc ggtgcgcagg gtcacccagg ccagggcagc tcccacgatg
     4621 aaaagttgcg gaagcaccac ggccgaggcc tccagcagat gagccgggtc gaggccgatg
     4681 acgtggaagg cggcgaacac cagggcggtg accacaatga tcgccctccg gctcctgtcg
     4741 cccattgact tcaagaggac tccccggaag atcagctcct cggtgacggg agcgatgacg
     4801 accgcgatga ccactgcagt gacccgagcc gccgtcgatt cgatgctggc cagggctgag
     4861 ccgacctgct gcaccgactc cccttcgggg aagaagaact gggtgagtgg gagaaagagg
     4921 acggcgagcc cgagttggag gaggagtccg agggcgaggt agcgggcatc cccgggctcg
     4981 atggagaacc cgagatcgga gtcgggcttt cttctcgcca gcagccagag gacgccgagg
     5041 tggccgacat actggccagc cagaccgagc accaggaacc agtcgccgtc gccccatatc
     5101 aggctgagac ctgcgaacac ccctgcacca aggaatccgc cgaggacgac aaagatgaag
     5161 tcgacgactg tccagggctt gggcacggac cggtgagcct actgactggg gcacccttcc
     5221 cactgttacc ctgcagccaa gcgtcgccgg aggatgg
//
LOCUS       DBNHXU010000329         2799 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2500473, whole genome shotgun sequence.
ACCESSION   DBNHXU010000329 DBNHXU010000000
VERSION     DBNHXU010000329.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2799)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2799
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2500473"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1636
                     /locus_tag="AC161X_08170"
     CDS             74..1636
                     /locus_tag="AC161X_08170"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013075326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0006637 - acyl-CoA metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA mutase large subunit family protein"
                     /protein_id="HIR4989565.1"
                     /translation="MDESRRTSSDIEVEILHEEPADPQRIGAPGEYPFTRGPHSTMYR
                     GRLWTMRQYAGFGTAEDTNRRFRDLLEAGQTGLSVAFDLPTQMGLDSDHPLAHGEVGK
                     VGVAVDTLDDMRRLLDGIPLDEVSASMTINATAPILLLLYQLVAEEQGVDASSLRGTV
                     QNDILKEYVARGTYIYPPGPSMRLVTDIFEYAQTEMPEWNTISISGYHIREAGATAAQ
                     ELAFTISNGLAYVRAAIGAGLDVDEFAPRLSFFWNGHNNFFEEVAKYRAARRIWARLM
                     KETIGASDPRSWAMRFHTQTAGSTLTAQQPLNNVVRTTIQALASVLGGTQSLHTNSYD
                     EALGLPTDESALLALRTQQVIAFETGVVDTVDPLAGSYFVEYLTDQLEAKADDLIEKI
                     ESLGGAVAAIEDGWMQAQIEESAYIEARRQSDSSSVVVGVNRFQEGDEAVVPVLDIDP
                     ALEEGQKRRLGDWRAGRDGAATGLALDNLEAEAATDHNLLPPMKAALAAGATVGEISD
                     RLRTVFGSHRPG"
     gene            1682..>2799
                     /locus_tag="AC161X_08175"
     CDS             1682..>2799
                     /locus_tag="AC161X_08175"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase family protein"
                     /protein_id="HIR4989566.1"
                     /translation="MADNRTHLVIIGGGPAGTQAATTAASKGARVTLIEKDIVGGAAH
                     LWDCIPSKTMAASALRHTSIRNAAKLGFVTEVGRVDPKVLSERIEVITGDINRNWMRL
                     LESQGVTMIEGTGRFVSDHEVAIATSDGERRLAFDKAMVSTGSAPRVPDWAPVDGKRV
                     LTTRDSYFLDEVPEHIVVIGSGVTGVEFTHIFVSLGSKVSLVVSRQQILPYRDAEVAA
                     VLEEDFLERGVRLVIGARAENIAVSSDGISVTCDDGRTISGSHALLAVGSYPLTDGLD
                     LDAAGIASDGGYITVDDFQRSSVPHIYAAGDVTGQMPLSSVASMQGRKIARHALDLPV
                     TPLDYSKVPEAIFTEPEIASVGLVDVDAAASGRKVRTM"
BASE COUNT          530 a          888 c          877 g          504 t
ORIGIN      
        1 ggggattgga cgtctcgggt gtcgcgagag ttcccgacct acgatccttg ggagatgtca
       61 ggataccccg actatggatg aaagccggcg cacctccagc gacatcgagg tcgagatcct
      121 gcacgaggag ccggcagatc cgcagaggat tggggcacca ggtgagtacc cgttcacccg
      181 cggcccccac tcaacgatgt accgaggccg actctggacg atgcgccagt acgccgggtt
      241 tggaacggcg gaggacacca accggcggtt tcgagatctt ctcgaggccg gccagacggg
      301 tctgagtgtc gccttcgacc tacctaccca aatgggcctc gactccgacc accctctcgc
      361 tcacggcgaa gtgggcaagg tcggggtggc cgtcgacacc ctcgacgaca tgcgtcggct
      421 cctggatggg atcccgctcg acgaggtctc cgcctcgatg accatcaatg ccaccgctcc
      481 catcctcctt ctcctctacc aactggttgc ggaggaacag ggggtcgatg cttcctcgct
      541 tcgggggacg gtccagaacg acatcctcaa ggagtacgtg gcccgtggca cctacatcta
      601 cccacccggg ccatcgatga ggctggtcac cgacatcttc gagtacgccc agacggaaat
      661 gcccgagtgg aacaccatct cgatcagtgg ctatcacatc agggaggcag gtgccaccgc
      721 agcccaggaa cttgccttca cgatttccaa cggcttggcg tacgtgaggg cagccatcgg
      781 cgccgggttg gatgttgatg agttcgctcc gagactgtcc tttttctgga acgggcacaa
      841 caacttcttc gaggaagtgg cgaaatatcg tgcagcccgc cggatctggg cccgactcat
      901 gaaggagacg atcggagcgt ccgatccgag gagctgggcc atgcgttttc acacccagac
      961 cgcgggctca acactcaccg cgcagcaacc actcaacaac gtggtccgca ccactatcca
     1021 ggcactggct tccgtcctcg gtggtaccca gtcgctgcac accaactcct acgacgaggc
     1081 gctcgggctg ccgaccgacg agtcggctct tctcgcattg cggacccaac aggtgattgc
     1141 cttcgagacc ggggttgtgg acaccgtcga tcctttggcc ggttcctatt tcgtcgagta
     1201 cctcaccgat cagttggagg ccaaggccga cgacctcatc gagaagatcg aaagcctcgg
     1261 aggtgcagtc gctgcgatcg aggacggctg gatgcaggct cagatcgaag agtccgccta
     1321 catcgaggca agacggcagt cggactcgag ttcggttgtc gtcggcgtca atcggttcca
     1381 ggagggtgac gaggcagtgg taccggtgct cgacatcgat cctgccctgg aggaaggcca
     1441 gaagcgccga ctgggggatt ggcgtgccgg gcgggacggc gccgccactg gattggccct
     1501 ggacaacctg gaggctgagg cggcgacgga ccacaacctc cttcctccga tgaaggcagc
     1561 cctggcggcg ggagccacgg tcggtgaaat ctcggatcgc cttcgaaccg tgttcggaag
     1621 ccatcgaccc ggttgattcc gccggcgtta tctgaggcgg ttaccgtccc gaccgattca
     1681 gatggcagat aaccgaactc acttggtgat catcggcggc gggcctgcgg gcacccaggc
     1741 cgccaccacc gcagcttcca agggcgcccg cgtcacgctg atcgagaagg acatcgtcgg
     1801 cggggcagct catctttggg attgcatccc ttcgaagacg atggctgcca gcgccctgcg
     1861 ccacacgtcg attcgcaatg cggccaagct tggttttgtg accgaggtgg gacgagtcga
     1921 ccccaaagtc ctgtcggagc ggatcgaagt catcaccggg gacatcaacc ggaattggat
     1981 gaggctcctc gaatcccagg gcgtcaccat gatcgaaggc accggtcgct tcgtgtccga
     2041 tcacgaggtg gcgattgcca cctcagatgg agagcgccgc ttggccttcg acaaggcgat
     2101 ggtgtccacc ggatcggcgc cgcgggtccc ggattgggcc ccggtcgacg gcaagcgggt
     2161 gctcaccacg agggacagct atttcctcga tgaggttccg gaacacatcg tggtgatcgg
     2221 gtcaggggtg accggcgtcg aattcaccca catattcgtg agccttggat ccaaggtcag
     2281 cctggtggtg tcacggcaac agatcctgcc atatcgcgac gccgaggtcg ctgccgttct
     2341 tgaggaggac ttcctggagc gcggggttcg gttggtgatc ggcgcccgcg ccgagaacat
     2401 cgcggtttca tccgatggga tttcggtcac ctgcgacgac ggccggacca tcagcggctc
     2461 tcacgccttg ctggcagtgg gctcgtatcc cctgaccgac ggtctcgacc ttgacgcagc
     2521 cgggatcgcc tccgacgggg gctatatcac cgtcgacgac ttccaacgca gttcggtgcc
     2581 gcacatctac gcagccggcg acgtcaccgg gcagatgcca ctcagctcgg tcgcgtcgat
     2641 gcagggcagg aaaatcgccc gtcacgcact cgaccttccc gtcaccccac tcgactactc
     2701 caaggtgcct gaggccatct tcaccgagcc ggaaatcgct tcggtcggcc ttgtcgacgt
     2761 ggacgcagcc gcgtcaggcc gcaaggtccg gaccatgaa
//
LOCUS       DBNHXU010000330         2877 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3095361, whole genome shotgun sequence.
ACCESSION   DBNHXU010000330 DBNHXU010000000
VERSION     DBNHXU010000330.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2877)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2877
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3095361"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..358)
                     /locus_tag="AC161X_08180"
     CDS             complement(<1..358)
                     /locus_tag="AC161X_08180"
                     /inference="COORDINATES: protein motif:HMM:NF014269.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HIR4989567.1"
                     /translation="MPETAQLTLPVLPLHNGVVFPHMVVTIRVETEEGKTALAAASSS
                     DGRLLLVPKLEGRYSTVGTIAEIQSDEDGSVVVSGLSRARVGAGTVDDSGALWVEAEA
                     YPEIDMIDDDLEDLIAE"
     gene            complement(493..2835)
                     /locus_tag="AC161X_08185"
     CDS             complement(493..2835)
                     /locus_tag="AC161X_08185"
                     /EC_number="3.1.6.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016883316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004065 - arylsulfatase activity [Evidence
                     IEA];
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arylsulfatase"
                     /protein_id="HIR4989568.1"
                     /translation="MSKRFKGVINIDDRDSVPDWEPYVQPVAPPGSPSVLYIVLDDVG
                     FSAMEPWGGLIETPNINRLAANGLTYTNWHTTALCSPTRSSLLTGRNHTTNGMACIAE
                     ATTGFPNANGHIPFECATIAEVLGDQGWNTYMLGKWHLVAADEMNMASTKRNWPVGRG
                     FERYYGFLGGETNQWFPDLIYDNHPVEQPSLPEDGYHLTVDLTDKALEFIKDAKTIAP
                     DKPFFMYFCPGATHAPHHAPKEWIEKYKGKFDMGYEAYREIVFENQKKMGIMPQDAVL
                     SPLNPYAEETSVDGKPWNPVDVVRPWDSLSEDEKALFCRMAEVYAGFLSHADHEIGRL
                     LDYLEDSGQLENTIVVLVSDNGASGEGGPNGSVNENKFFNGIPDSMEENLQYLDQLGS
                     TATYNHYPVGWAWAFNTPFKMWKRYNFEGGVADPLVISWPKGIKASGEIRDQFCHATD
                     IVATMYDCLGVELPEEVKGFHQIPLEGVSIRSTFESADTPTPKETGFFSMLGSRAIWH
                     KGWKAVSVHPTISGWGHFDQDRWELYDTEKDRTEMNDLAADNPVKVKEMINLWFHEAG
                     LYNGLPLLDKTAVEVLADPTRPQVAPPRDRYVYYPGTAEVPETAAVNVRNRSYSIAVE
                     TTVDSEDASGVLFSHGARFGGHSLYIKDRKLKYVYNFVGSNEQIIESTKEIPTGNVIF
                     GASFEKEGDAMPTTGTLSLFVDDEKVGEGKIMTQPGNFSLVGEGLNVGKDGGEPVTSD
                     YSGSHPYAFTGGTIKEAIVDVSGDQYIDLEMEALAMMKRE"
BASE COUNT          520 a          862 c          865 g          630 t
ORIGIN      
        1 actcggcgat cagatcctcg aggtcatcgt cgatcatgtc gatttcggga taagcctcgg
       61 cttcgaccca aagggcaccg ctgtcgtcga ccgtcccggc tccgacgcgg gcgcgggaca
      121 gacccgatac cacgacggat ccgtcctcat cggattggat ctcggcaatg gtgcccacgg
      181 tcgaatagcg accctcgagt ttggggacca gtagcaagcg gccatcactg ctggaggcgg
      241 cagcgagggc agtcttgccc tcttcggtct ccacccggat ggtgaccacc atgtgaggga
      301 acaccacacc gttgtggagg ggaaggacgg gaagggtcag ttgggcagtc tcaggcattg
      361 ggggccaaat tccttcttga aatacgggtg gttgaacctc ttccaaccac gtcgtaacca
      421 gccctattcc agaaacctga gtcttgatcg ctcaggttta ctgttcccca tcccgagaag
      481 gtctgagagg tgtcactccc gcttcatcat ggcgagggct tccatctcca ggtcgatgta
      541 ctgatccccg gagacgtcga ctatcgcttc ttttatcgtg ccgccggtga acgcgtatgg
      601 gtgcgacccg ctgtagtcgg aggtcaccgg ctcgccacca tccttaccca cgttcagccc
      661 ctcaccgacc agtgagaagt tcccaggctg ggtcatgatc tttccctccc cgaccttctc
      721 atcgtcgacg aacaaggaca acgtcccggt ggtcggcatc gcgtcgcctt ccttctcgaa
      781 tgatgctccg aagatcacat tccccgtggg gatctccttg gtcgactcga tgatctgctc
      841 atttgagccg acgaagttgt agacgtactt gagcttgcgg tccttgatgt acagcgagtg
      901 gccaccgaac cgggcaccgt gtgagaagag cactcccgag gcgtcctcgg agtcgacagt
      961 ggtctcgacc gcgatcgaat agctgcgatt gcggacgttg actgccgctg tctctgggac
     1021 ctcggccgtg cccgggtagt agacgtacct gtctctgggg ggcgcaacct gaggccgggt
     1081 ggggtcggca aggacctcaa ccgcggtctt gtccaggagc ggaaggccgt tgtacaaacc
     1141 ggcctcgtgg aaccagaggt tgatcatttc cttgaccttc acggggttat ccgcggccag
     1201 gtcgttcatc tcggtgcggt ccttttcggt gtcgtagagc tcccaccggt cttgatcgaa
     1261 gtggccccac ccagagatgg tcggatggac cgaaaccgct ttccacccct tgtgccagat
     1321 ggccctcgac ccgagcatcg agaagaaccc ggtctccttc ggcgttgggg tatcggcgct
     1381 ctcgaaggtc gagcggatgc tcaccccttc tagtgggatc tgatgaaagc ccttgacttc
     1441 ctccggcagc tctaccccga ggcagtcgta catggtggcg acgatgtcgg tggcatggca
     1501 gaactggtcc cggatctcac cactcgcctt gatccccttc ggccaggaga tgaccagcgg
     1561 atcggcgact ccgccttcga agttgtacct cttccacatt ttgaacgggg tgttgaacgc
     1621 ccaggcccat cccaccgggt agtggttgta ggtcgcggtc gatcccaatt ggtccagata
     1681 ctggaggttc tcctccatcg agtccgggat cccgttgaag aacttgttct cgttcaccga
     1741 cccgttgggc ccaccctcgc cgctggcgcc gttgtccgac accaatacca cgatcgtgtt
     1801 ctccagctga cctgagtcct cgagatagtc gagcagccga ccaatctcgt ggtcggcgtg
     1861 gctgaggaaa ccggcgtata cctccgccat ccgacagaac aacgccttct cgtcctcgga
     1921 gagcgaatcc cagggacgga cgacatctac cgggttccat ggctttccgt cgacgctggt
     1981 ttcctccgca tagggattga ggggagagag cacggcgtcc tggggcatga tccccatctt
     2041 cttctgattc tcgaacacga tctctcgata ggcctcgtag cccatgtcga acttgccctt
     2101 gtacttctcg atccactcct tcggagcgtg gtgtggtgcg tgtgtggcac cagggcagaa
     2161 gtacatgaag aacggtttgt ccggtgcgat ggttttggcg tctttgatga actcgagcgc
     2221 cttgtcagtg aggtcgacgg tgaggtggta gccgtcctcg gggagggatg gctgctccac
     2281 cgggtggttg tcatagatca gatccgggaa ccactgattg gtctcgccgc caaggaaccc
     2341 gtagtagcgc tcgaagccgc gtcccaccgg ccaattgcgc ttggtggagg ccatgttcat
     2401 ctcgtcggcg gccaccaggt gccacttgcc gagcatgtag gtattccagc cctggtcccc
     2461 caagacctcg gcgattgtgg cgcactcgaa ggggatatga ccgttggcgt tcgggaagcc
     2521 agtggttgcc tcagcgatgc acgccattcc attggtggtg tggttgcgac ctgtgagcaa
     2581 tgaggaacgg gttggcgagc acagggcagt tgtatgccaa ttggtatacg tgagcccgtt
     2641 ggccgccagg cgattgatgt tgggtgtctc gatgagcccg ccccagggct ccattgccga
     2701 gaagccgaca tcgtcgagaa cgatgtagag gacgctcggc gagccgggag gagccaccgg
     2761 ctggacgtaa ggctcccagt ccgggaccga gtcgcgatcg tcgatgttga taacgccttt
     2821 gaaccttttg ctcactttcg cccctttcgg atctggaccc caacatgaga gccttct
//
LOCUS       DBNHXU010000331         2697 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2698938, whole genome shotgun sequence.
ACCESSION   DBNHXU010000331 DBNHXU010000000
VERSION     DBNHXU010000331.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2697)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2697
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2698938"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1874)
                     /locus_tag="AC161X_08190"
     CDS             complement(<1..1874)
                     /locus_tag="AC161X_08190"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4989569.1"
                     /translation="MTSRDLPVGTVSFVFTDIEGSTRLLQDLGDAFPTVLERHNDLVL
                     AAVKEHQGLMVKSEGDGFFLAFQSAVDAVRCAVDIQRSMAAEPWATPRPVRVRIGIHT
                     GEGRLGGSDYVGIDVHRAARISDSGHGGQILLSETTTRLAEYSLPERVRIENLGNHRL
                     KDLTHEEHLYQLIIEGLESEFPALRTVSGARGNLPRRDLQLIGRIEEQTAVAEALERS
                     RLVTITGPGGVGKTSLALKVSADLAESFADGVWFVEVSRVADETLLAASVARQLHITE
                     SPHQPLIDTLASRLGKARALLILDGCEHLIDAVAKLSDFLLGATTELKILATSRELLS
                     IREEHLVHVGPLPIPGTGTLSVAEIATFDSVALFVERARLVSPGFVLDGQNATFVAEV
                     CRRLDGIPLAIELAAARLKVLSAAQLVQRLDQQFALLSGTLRDMPPHQQTLETTLDWS
                     FDFLSGAERLLFKRLSIFSGGFTLEAAEQICSGDLVDRSFVMNLLGRLVETSLVMAVG
                     AENQRYRMLEPISHYAKMRLDAEGRNDELLDRHARFFTDLAEQADMQLLGTAQTDAAD
                     HLELEHYNLRNALDWLFRSGDYESAIALAGALRWFWVIKREVSEGTEWIERTLTKREG
                     A"
     gene            complement(1909..>2697)
                     /locus_tag="AC161X_08195"
     CDS             complement(1909..>2697)
                     /locus_tag="AC161X_08195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004923208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HIR4989570.1"
                     /translation="SRRTFRSTSFSRDTAGMDIEQLLALGRQLLEAEPMGRAQLGKLL
                     AERWPERDPASMAYAVSYLLPLVQVPPRGLWSGKGAAKWTTVQHWLGAKPGPKSAIES
                     LVLRYLAAFGPASVSDIRVWSGVTGLAEVVERLRPRLRVFTDESGRDLFDLPEAPRPD
                     PDTPAPVRFLPEYDNVLLGHADRSRVIEPGARPPGWAGNLLHDGFLRGSWKIATTKQV
                     TTITITPFARLSKETADEVQLEASHLLRLVAPDSPHEIQLAIPG"
BASE COUNT          490 a          817 c          891 g          499 t
ORIGIN      
        1 gcgccttccc tcttcgtcag ggtccgctcg atccactcgg tgccctcgga gacctcgcgt
       61 ttgatgaccc aaaaccagcg caaggcgccg gcgagggcta tcgcgctctc gtagtcgccg
      121 gacctgaaca gccagtccag ggcgttgcga aggttgtagt gctccagttc caggtgatcg
      181 gcagcatcgg tctgcgccgt tcccagaagc tgcatgtctg cttgctcggc caggtcagtg
      241 aagaagcgag cgtgccggtc gagcaactca tcgttgcgtc cctcggcgtc gagtcgcatt
      301 ttcgcgtagt gggagatcgg ctcgagcatt cgatagcgct ggttctcggc gccgactgcc
      361 atgaccaggg atgtctcgac gagccttccg agcagattca tgacgaagct ccggtcgacc
      421 agatcgcccg aacaaatctg ttcggccgct tccagggtga agccgcccga gaagatcgac
      481 agtcgtttga aaaggagccg ctcggcaccg gagagaaagt caaagctcca gtcaagagtg
      541 gtctccaagg tctgttggtg ggggggcatg tcccgaagcg tccctgagag gagggcgaac
      601 tgctggtcga ggcgctggac cagttgagca gcggacaaca ccttgagccg ggccgcagcc
      661 agttcgatgg cgagcgggat tccgtcgagt cggcgacaga cctcggctac gaaggtcgcg
      721 ttttggccgt cgagcacgaa accgggggat accaggcggg cccgttcgac gaagagggca
      781 actgagtcga aggtcgcgat ctcggcaacg gatagcgtgc cagtcccggg aatcggcagc
      841 ggcccgacat gcaccaggtg ctcttccctg atggacagaa gctcgcggct cgtggcgagg
      901 atctttagct cggtggtggc gccgagcagg aagtcggaca gcttcgccac cgcatcgatc
      961 aggtgctcgc acccatcgag aatcagcagg gcgcgcgcct tgcccaatct ggaggccagg
     1021 gtgtcaatca agggctggtg agggctctcg gtgatgtgta gctgacgggc gacgctcgca
     1081 gccagcagcg tttcgtctgc cactcgcgag acctcgacga accagacgcc gtcggcgaag
     1141 gactctgcaa ggtcggccga gaccttcaga gccaaactcg tcttgccgac gcctccgggc
     1201 ccggtgatgg tgacgaggcg actccgctct agggcctcgg cgaccgccgt ctgttcttct
     1261 atgcggccta tcagttggag gtcgcgcctc ggaagattgc cccttgctcc cgagaccgtg
     1321 cgtaacgctg ggaattcgga ctcgagccct tcgatgatga gctgatagag gtgctcctcg
     1381 tgggtcaggt ctttgaggcg gtggttgcca aggttttcga tccggacccg ttctggcagg
     1441 gagtactcgg cgagccgggt tgtggtctcc gacagcagaa tctggccgcc atggccgctg
     1501 tcactgatcc gcgctgcccg gtggacgtcg atccccacat agtcggatcc cccgagtctg
     1561 ccctcgccgg tgtggatccc gatccgcacc ctgaccggcc tgggtgtagc ccagggctca
     1621 gccgccatgg atcgttgaat gtcgaccgca caccgcaccg catcgaccgc tgactggaag
     1681 gcgagaaaga atccgtcgcc ctcgctcttg accatcaacc cttggtgctc cttgacggct
     1741 gcgaggacca gatcgttgtg acgctccagg accgtcggga aagcatcgcc aaggtcctgg
     1801 agaagacgcg tcgaaccctc gatgtcagtg aagacgaagc tgacggtgcc gaccggcagg
     1861 tctcgggaag tcatggttga ccccaaagta acaagccctc aacctgcctc agccggggat
     1921 tgccagctgg atttcgtgtg gcgagtcggg ggcaacgagt cgaagaaggt gggacgcctc
     1981 tagctgtacc tcgtctgcgg tctccttgga gagccttgca aacggggtga tcgtgatagt
     2041 tgtgacctgt tttgtggttg caattttcca gctgccacgg agaaacccgt cgtgcaagag
     2101 gttgcccgcc catccgggtg gcctggcacc cggctcgatc acccgcgacc ggtcggcgtg
     2161 acccaggagg acattgtcgt attcggggag gaaccgaacc ggtgccggag tgtccgggtc
     2221 cgggcgtggc gcctcgggga ggtcgaacag atcgcgtccg ctttcgtctg tgaatacccg
     2281 gagccgaggg cgaagcctct ccactacctc ggcgagaccg gtcaccccgg accagaccct
     2341 gatgtcgctg accgatgccg gaccaaatgc agccaggtat ctgagcacga gtgactcgat
     2401 cgcggacttg ggcccgggct tggctcccag ccagtgctgc acagtggtcc acttggcggc
     2461 gcccttcccg ctccagaggc ctcggggcgg cacttggacc aagggaagga gataggagac
     2521 tgcataggcc atcgaggccg gatcgcgttc gggccacctt tctgcgagga gcttgcccag
     2581 ctgggcccga cccatgggtt ctgcttcaag cagctggcgt ccgagagcga gcagctgctc
     2641 gatgtccatt ccggccgtat ccctggagaa tgaggtcgag cgaaaagttc gtcggga
//
LOCUS       DBNHXU010000332         3151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_914959, whole genome shotgun sequence.
ACCESSION   DBNHXU010000332 DBNHXU010000000
VERSION     DBNHXU010000332.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3151
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_914959"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..165)
                     /locus_tag="AC161X_08200"
     CDS             complement(<1..165)
                     /locus_tag="AC161X_08200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4989571.1"
                     /translation="MSVTTPVIEVEGLVKNYEDRVAVAGIDFTVSPGEIFGVLGPNGA
                     GKTTTILMLLG"
     gene            complement(162..1328)
                     /locus_tag="AC161X_08205"
     CDS             complement(162..1328)
                     /locus_tag="AC161X_08205"
                     /inference="COORDINATES: protein motif:HMM:NF022100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="COG1470 family protein"
                     /protein_id="HIR4989572.1"
                     /translation="MKRLFHVLAVGLLATATLALPAMAQETTTPPPTTLSINSPFVGV
                     AVKPGDSATFKLTVGAVPGDRVAFALAGVPDGWDAELRGGGFVVDEVQVDSTGFVSLD
                     LQIDVPAETEEGSYEMSLTGTGSSGTDRLDLSIRVAATVGGDVSMTTDFPNLQGPADS
                     TYSFDVTITNDTPQDIQFGLSATGPEGWVSEIKPSGEAQASTVTVAAGSSGQVTVDVD
                     PPDAAAAGDYAITAKAEGGGVSAEVELGVRITGSFGIDIATANEALNVKVKGEQATDL
                     PLVVTNTGTAPLLGVNLTATPPQGWDVTFDRATIDQLEPGQALQVVATITPSGEAING
                     DYAITFTSSVAEATDSIDVRTTVETSAIWGLVGIGVIVIALVGLSMVFRRYGRR"
     gene            complement(1438..2154)
                     /gene="deoD"
                     /locus_tag="AC161X_08210"
     CDS             complement(1438..2154)
                     /gene="deoD"
                     /locus_tag="AC161X_08210"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010626222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HIR4989573.1"
                     /translation="MPTPHISAEKGDFAPSILLPGDPLRAKHIADTFLDGARQVNAVR
                     NMLGFTGTFNGVPVSVMGTGMGIPSASIYATELITEYGVERLVRVGSCGGILSSVKVR
                     DVILAIGASTDSGVNRARYGGMDFAATADFGLLMAAFDAAAEKGIEVKVGNVHSADTF
                     YNPNPATFDLMERMGILAVEMEAAGLYGVAAEKGARSLTIATVSDHIRTGEATSPEER
                     EQTFNEMVEIALEAVRRDVG"
     gene            complement(2193..>3151)
                     /locus_tag="AC161X_08215"
     CDS             complement(2193..>3151)
                     /locus_tag="AC161X_08215"
                     /inference="COORDINATES: protein motif:HMM:NF012640.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="chorismate-binding protein"
                     /protein_id="HIR4989574.1"
                     /translation="RHPTLWQMTSTITARMPADLGLGGLFTGIFPSASVTGAPKVSTM
                     GIIADLEPDARGVYCGAIGYLEPGGERAEFSVAIRTGVVSDGRFTYHVGGGITYDSLA
                     GAEYDECLWKALVVTEPPDTPDLLETMRYEPGEGIPLLERHVARLAASAAYWGIPFDP
                     VAVGDALSTVGGPVALKVRLVLGASGTAEVSSELLPSWEEPVGLAIWESRVDQSDPRW
                     AHKLDDRSRYPIGDDGIEVVMLNLDGEITETNISNLMLRFDHIWLTPAVSSGCLPGVQ
                     RELALDLGLASEASLTIEDMNRADEIAVTNAVRGWRKAVLIE"
BASE COUNT          597 a          999 c          977 g          578 t
ORIGIN      
        1 accgagcagc atgaggatgg tcgtggtctt cccggcgccg ttgggcccga gaaccccgaa
       61 gatctcaccc ggtgaaaccg tgaagtcgat cccggccacg gcaaccctgt cctcgtaatt
      121 cttgaccagg ccctcgacct cgatgaccgg tgtggtcacg ctcacctccg cccgtagcgt
      181 cggaacacca tgctgagccc gacgagggca atcacgatca cgccgatccc cacgaggccc
      241 cagatggcgg aggtctcgac cgtggtgcgg acatcgatgc tgtccgttgc ctcggcaaca
      301 gatgaggtga aagtgatcgc gtagtcgccg ttgatcgctt ccccggatgg ggtgatggtt
      361 gcaaccacct gaagggcctg acccggctcc agttggtcga tggtggcgcg atcgaaggtg
      421 acgtcccagc cctgtggcgg ggtcgcggtc aagttcacgc cgaggagcgg agcggtgccg
      481 gtattggtca ccaccagcgg gaggtcggtc gcctgctcgc ccttcacctt cacgttcagc
      541 gcttcgttgg cagtcgcaat gtcgattccg aacgagccgg tgatccgcac gccgagctcg
      601 acttcagcgg acacaccgcc accctcggcc tttgcggtta tggcgtagtc acccgccgca
      661 gcggcatccg gcggatcgac atccactgtg acctgaccag atgagcccgc ggccacggtg
      721 accgtggacg cctgcgcctc gcctgagggc ttgatctctg acacccagcc ttcaggtccg
      781 gtcgccgaaa gcccgaactg aatgtcctgg ggagtgtcgt tggtgatcgt cacatcgaag
      841 gagtaggtgg agtcggccgg gccctggagg ttcgggaagt cggtggtcat cgagacatcc
      901 ccgcccactg tggccgcgac ccgaatcgag agatcgaggc ggtcggtccc cgagctgcca
      961 gtgccggtca acgacatctc gtatgagccc tcttcggttt ccgccgggac gtcgatctgg
     1021 aggtcgagcg acacgaatcc tgtggaatcg acctgaactt cgtcgacgac aaacccgccg
     1081 ccacggagct ccgcatccca tccgtcgggg actccggcca aggcgaaagc gacccgatct
     1141 cctggaactg cgccaaccgt cagtttgaag gtggcactgt cacccggctt aacggcaacg
     1201 ccgacgaacg gggagttgat cgaaagggtg gtaggtggag gagtcgtcgt ctcctgggcc
     1261 atcgcaggga gggccagcgt cgctgtggcc agcaacccaa ctgctaacac gtggaaaagt
     1321 cgcttcactt tcatctccgt ttggatccag gtctctcttg gctcagttca acagccaccg
     1381 aagggggaat ctcgctcagc ccacgtttag aagatataaa ggcttggcag ggtaggccta
     1441 accgacatcc cgtctgaccg cctcgagggc aatctccacc atctcgttga aggtctgctc
     1501 gcgctcctcc ggactggtcg cttcgccggt gcggatgtgg tcagagacgg tggcaattgt
     1561 caacgagcgg gcacccttct ctgctgccac tccgtagaga ccggcagctt ccatctccac
     1621 cgcgaggatt cccatcctct ccatcaggtc gaaggtggcc gggttggggt tgtagaaggt
     1681 gtcagcggag tggacattgc ccacctttac ctcgatcccc ttttcggcgg cagcgtcaaa
     1741 ggcagccatc aacagaccga aatcggcggt ggcggcgaag tccatacccc catagcgagc
     1801 ccgattgacg ccggagtcag tggaggctcc gatggcgagg atcacatctc tgaccttgac
     1861 cgaggaaagg atgcctccac aagatccgac ccggaccagc cgctccaccc cgtattcagt
     1921 gatcaactca gtggcataga tagacgccga aggaatcccc atcccggtcc ccattactga
     1981 gaccggcacc ccgttgaagg tgccggtgaa gcccagcatg tttctgaccg cgttcacctg
     2041 cctggcgccg tcgagaaagg tgtcggcaat gtgcttggcc cggagcggat cgccgggaag
     2101 gaggatcgat ggggcgaaat cccctttctc ggccgagatg tggggggttg gcatgaaagg
     2161 gggctcctga actgtgatcg gtcgcccaca acttattcga tcagaactgc tttcctccat
     2221 cctcggaccg cgttggtaac cgcgatctcg tcggctcgat tcatgtcttc gatcgttaga
     2281 gacgcctcac tggcgagacc gaggtccaag gcaagctccc gctggactcc gggaaggcac
     2341 ccggaggaca cggccggggt tagccatatg tggtcgaagc gcaacatcag gtttgagatg
     2401 ttggtctccg tgatctcccc gtcgagattg agcatcacga cctcgattcc atcgtctcct
     2461 atcgggtagc gggaacgatc gtcgagtttg tgcgcccacc tcgggtcgct ctggtccacc
     2521 cgagactccc agatcgcgag gcccaccggc tcctcccatg agggaaggag ctctgagctg
     2581 acctcggccg tgcccgacgc cccaagcacg agccttacct tgagggcgac cggcccgccg
     2641 acggtcgaga gtgcatctcc gacggcgacc ggatcaaagg gaatgcccca ataggcggcg
     2701 gaggcagcga gtcgggcgac gtgccgctcc agcaatggga tcccttctcc aggctcatat
     2761 ctcatcgtct ccagaagatc cggcgtatcg ggaggctcgg tgacgaccaa ggccttccaa
     2821 aggcactcgt cgtactcggc tccggcgagg gagtcatagg tgatcccgcc gccgacgtga
     2881 taggtgaatc gaccgtcgct cacgaccccg gttcggatgg cgaccgagaa ctcggccctt
     2941 tcgccgccgg gttcgagata gccaatcgcc ccgcagtaga cccccctcgc atccggctcg
     3001 aggtcggcga tgatccccat ggtcgacacc ttcggagcgc cagtcaccga agccgaggga
     3061 aagatccctg tgaagagccc acccaggccg agatcggcgg gcatgcgggc ggtgatggtc
     3121 gacgtcatct gccaaagggt gggatgacgt t
//
LOCUS       DBNHXU010000333         7925 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4487700, whole genome shotgun sequence.
ACCESSION   DBNHXU010000333 DBNHXU010000000
VERSION     DBNHXU010000333.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 7925)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 7925)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7925
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4487700"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1627)
                     /locus_tag="AC161X_08220"
     CDS             complement(140..1627)
                     /locus_tag="AC161X_08220"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF024777.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HIR4989575.1"
                     /translation="MTIIGTTLDRTAPEQPYSRIRLTLGVLGRAWMWFVGACLLITIL
                     PMAFGWHPYVILTGSMAPNIEAGDIVLSSPDPQPDQLLGRVVVFEDPNKADAVLTHRV
                     IGFNGDGTMTTKGDANPTPDSTPVQVDAVDGLGRLLVRYVGLPVVWARTGNWALIVLH
                     LALIIGAMVAAAHDYEPDDDDDPEPPGTPATVPSGGWLREVIHSPASFVALIALAGVL
                     AIPTTGAAFTASTTNSADTWSIPNWSYTTEVNALGPYLYWKLDETGTATTAADSSGNG
                     RTGTYNPSGGATDFTRLADGALVTDTPDRAVQLGGNACINTTSTTAINAPQVFTVVAW
                     FKAPASYTNGGKLIGFERPQTGTAVPTAGAYDRHIYMDGNGRIWFGVYNGADVTLSSG
                     TGLNDNQWHMAVGTQSASGMRLYIDGAQVGSNTNTVAETQTGWWRAGCGNLSGWGNEW
                     GGANNPGTNSATTQNRVFAGSLDEIAVFSGTALTAQQVSFLYWAR"
     gene            complement(1712..2353)
                     /locus_tag="AC161X_08225"
     CDS             complement(1712..2353)
                     /locus_tag="AC161X_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989576.1"
                     /translation="MATITSTSDRRQRRMLRAAAPLAGLLVAALLVWQGSNAAFSATT
                     DNTGDAWATGNLLLTNNGGGAAYSGTTAALFTENNLKPGNTGFKCITVESTGSLAGNL
                     RLYRGAITGTNSAALSAVLDVTIDAVAVAAATNVQANCTGYTGGSGGALYNGTLSAMP
                     ATYAGASGMAVAGGTQRVAYRIGWTVNAGAGNSVQSSSAVANLVWEINSGSNR"
     gene            2557..3129
                     /locus_tag="AC161X_08230"
     CDS             2557..3129
                     /locus_tag="AC161X_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989577.1"
                     /translation="MAVVVGVTIPASLLVAALAGWVGLVIVGVVLILMWRLSPGLWRI
                     FGIGLLGGAIAGVLILGPGFRLAMRVVAIVDVRRVEFSVAGTMFIIVLLGVIVGGLGG
                     VFSAMLRKGLGWSSLVMSLLMTVVLVALLVIDTGLRDELVTLGAGPWLNIPMFAAVCF
                     GYSLFANRLIDRFERKKSRPAAREPVEVPT"
     gene            3155..3538
                     /locus_tag="AC161X_08235"
     CDS             3155..3538
                     /locus_tag="AC161X_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989578.1"
                     /translation="MSWWAIELITNVILVGLVVFLGPLIKRFGRSYAGDVFRGNPRTG
                     KSYLVLMDVAYYLIFFALILFTVSFQDQWNDVGEQLESELRRVGGMLLLMGVLHGLNV
                     LTLPLVGRLLGLGRQLEEDTPPPVT"
     gene            3541..4089
                     /locus_tag="AC161X_08240"
     CDS             3541..4089
                     /locus_tag="AC161X_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009777390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4989579.1"
                     /translation="MYWAEALDNTVIWTAETLDRRAVMESLYKQEAPSLVRLARLFTD
                     DRTAAEDLVQEGFIRLFHSAHRIDDPGKAAAYLRSIVLNLARDHNRRGLLSLRHQDAL
                     TEIAPPEQPEEQVVRSESEAQVLDALRSLSARQRDCLVLRFYMDLSEKEIASTLRISP
                     NSVKTHCQRGMAALASILGEES"
     gene            4086..4826
                     /locus_tag="AC161X_08245"
     CDS             4086..4826
                     /locus_tag="AC161X_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989580.1"
                     /translation="MTLEQRLIETLHRADDFEPSPDLFARVERSLEEDRRHRRRITWV
                     AAGTLAGAVAASLLLRATISHSALGELIAPGWVLELIVTLAKVALILVLGPNLRRLGL
                     PLIADVFRLDPPTGKRFLKLLDIAFYLFLFGQILLEAYPQLWDQEVLLGEDGWLWTGS
                     MAIFMVMMGLGHIATIAALPVIGLFHSSLVRRNRRWEAGSGAPPRSEGAEQAERVVRL
                     IVWGIAGLALLSVLIAVGVLFVGGVVDA"
     gene            4926..5780
                     /locus_tag="AC161X_08250"
     CDS             4926..5780
                     /locus_tag="AC161X_08250"
                     /inference="COORDINATES: protein motif:HMM:NF042511.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6544 family protein"
                     /protein_id="HIR4989581.1"
                     /translation="MVYSRDLGGPGARARVATPKPNRSLYRRFLKDVADAGLPSGPGP
                     QEPVGEDDLASLPATARRYMEFMGVPGRPRDWSLRARFVGRFRLRPHADWLPAEAWQY
                     NTALEVGRVYTMRIRFASVLPMTGKDTYLKGHGQMLGKLVGLFTVAEGQGPEFDIGEL
                     TTYLNDAILLAPSFLLGPNTTWAEVDDESFEVTLADSGRHVTARVFVDERGAPTDFST
                     TDRFADLPGGLVRAKWTTPTPVWLSGSGRPVPGPMSAVWHLADGPLPYIEGRLIPESI
                     NYNVAPEA"
     gene            complement(5793..6905)
                     /locus_tag="AC161X_08255"
     CDS             complement(5793..6905)
                     /locus_tag="AC161X_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989582.1"
                     /translation="MSRSRLGLLLVFAMLSTLIPIGIAPAAATTGDLVISGVIQGPRA
                     GGNPKAVEFLVVNNIPNLGVYGFGSANNGGGTDGQEFTFPSGPASAGDFIYVSLEGTQ
                     FASFFGFAPDFMTTAINVNGDDAIELFQNGVVVDVFGVINVDGTGQPWEYTNGWAYRK
                     LGTGADGSTFDLSSWNFSGVNALVGTTLTNATATSPFPRGSYVATAPTTTTTSTSTTS
                     TTSTSTTTTTTSTTSTTSTTTTTTLYVPPPTLPTLPPSPGKDVKAPKVHADFDRFWTD
                     HGRGWFKVDFSCWDRVDRRPSCLGSINGIEVRDGQKVYLVKTDGLASSYRAHGVLFIK
                     APAFLLTVTGTDRSGNSASATAEPWFKYRYQHRHWC"
     gene            complement(7090..>7925)
                     /locus_tag="AC161X_08260"
     CDS             complement(7090..>7925)
                     /locus_tag="AC161X_08260"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:NF044379.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_function: GO:0097216 - guanosine tetraphosphate binding
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="large ribosomal subunit assembly factor BipA"
                     /protein_id="HIR4989583.1"
                     /translation="DRLKREVLGNVSIRLGPTSSPEIVEVAGRGELQLAVLIESMRRE
                     GFELQVSRPEVVVRMIDSSPHEPVERAVVDVPDEYVGAVTQAAAPRKGTIRELQPGDP
                     GRTVITFTAPARGLLGFRSLLMTTTRGTALIHQHHEGWMPWVGELPHRIGGAMVSDRK
                     GTSTGFALDNLQKRGELFIGAGEETYEGMIIGEASRPDEMVVNAARPKQLTNIRTHAS
                     DEAINLRPPREMTLETAIEWIADDELVEVTPSSIRVRKRFLSEPERRRLGKKSVSTVS
                     G"
BASE COUNT         1319 a         2378 c         2607 g         1621 t
ORIGIN      
        1 agatacccgg aatggagatc tcgttgctcg aggcgctcac gggtgacgcc gccgttccct
       61 accgagaggg gcgggcgatg ggtttcgacg aagcggtggc ctacgcgttg gcgggtccca
      121 ccgacgaggg attcggggct cagcgggccc agtagagaaa actcacctgc tgcgcggtga
      181 gggccgtgcc ggagaacact gcgatctcgt cgagcgaccc cgcaaacact ctgttttgcg
      241 tggttgccga gttggtgccc gggttgttgg caccgcccca ctcgttcccc caacccgaaa
      301 ggttgccaca ccccgcccgc caccaaccgg tctgggtctc ggcgacggtg ttggtgttgg
      361 aaccaacctg ggccccgtcg atgtagagcc gcatccccga tgcgctctgg gtaccgacgg
      421 ccatgtgcca ctggttgtcg ttgagccctg tcccggagct gagcgtgacg tctgcgccgt
      481 tgtaaacccc aaaccaaatc cgcccgttgc catccatata gatgtggcgg tcgtaggcgc
      541 ctgccgtcgg caccgccgtc ccggtctgtg gcctctcgaa cccgatcagc tttccgccgt
      601 tggtgtacga ggccggtgct ttgaaccagg cgacgacggt gaacacttgc ggggcgttga
      661 tcgccgtggt cgatgtggtg ttgatgcagg cattgccgcc aagttggact gcccgatccg
      721 gagtgtccgt caccagcgcg ccgtcggcga gccgggtgaa gtcggtcgcg ccgccgctcg
      781 ggttgtaagt gccggtgcga ccgttgcccg aggagtcagc tgcggtggtg gcagtcccgg
      841 tctcgtccag tttccagtag agataggggc cgagggcgtt gacctcggtt gtgtatgacc
      901 agttagggat gctccacgtg tcggcgctgt tggtcgtgga agcggtgaag gcggcgccgg
      961 tggttggaat ggcgagtacg ccagcaaggg caatgagtgc aacgaaactc gccggcgaat
     1021 ggatcacctc acgcagccag cctccggaag gcactgtcgc cggcgtgcca ggaggttcgg
     1081 gatcgtcgtc gtcatcaggc tcgtagtcgt gagcggcggc caccatcgcc ccgatgatca
     1141 aggccagatg gaggactatc agggcccagt tgccggtccg ggcccagacc actggcaggc
     1201 cgacgtagcg gaccagcagc cgtccgagac cgtcgaccgc atcgacctgg accggcgtcg
     1261 agtccggtgt gggattggcg tcgcctttgg tggtcatggt cccgtccccg ttgaagccaa
     1321 tcacccggtg cgtgagaacg gcgtccgcct tgttggggtc ttcgaacaca accactcttc
     1381 ccaagagctg gtcgggttga ggatccggtg aggagagaac gatgtcaccg gcctcgatgt
     1441 ttggcgccat ggagccggtg aggatgacgt acgggtgcca accaaaggcc atggggagga
     1501 tggtgatcag aagacaggcg cccacaaacc acatccaggc ccggccgagg acacccaggg
     1561 tcagacggat tctgctgtaa ggctgctccg gggctgtcct atcgagggtg gtgccgatga
     1621 tggtcatggt gtgcggttgg tcactttccc tgaaacggct gccggccccg gcccgggaac
     1681 tccaggggcc ggggccggca cggttcagga ctcagcggtt tgaccccgag ttgatctccc
     1741 acaccaggtt ggcgacggcg ctcgagctct ggacgctgtt gcctgcgcca gcattcacgg
     1801 tccagccgat ccggtaggcc acacgttgcg tgccgccggc cactgccatc ccggaggctc
     1861 cggcataggt ggcgggcatg gcgctcaggg tcccgttgta gagggcccca ccgctgcccc
     1921 cggtgtagcc ggtgcagttg gcctggacat tggttgcagc cgccactgcc accgcgtcga
     1981 ttgtgacatc gagcactgcc gagagggctg cgctgttggt gccggtgatg gcaccgcggt
     2041 agagcctgag gttcccggcg agggatccag tcgactcaac ggtgatgcac ttgaacccgg
     2101 tgttgccggg cttgaggttg ttctctgtga acaaggctgc ggtggttccc gagtatgccg
     2161 ctccaccacc gttgttcgtg aggagaaggt tgccggtcgc ccatgcatcg ccggtgttgt
     2221 ccgtggttgc gctgaacgcg gcgttgctgc cctgccagac gaggagagca gctaccagaa
     2281 gaccggccag aggggcggct gccctgagca tccttctctg ccgccggtca gatgtcgatg
     2341 tgattgttgc cattgtccca aacctttctt gatctatcag tccctcaaga gatcggaacg
     2401 attggtggcc tggaagtagg acggtcctgg atgtctccta gatgactagt catcggtggg
     2461 tatatcggcc taggcgggct gtcacccgtt gctgcggtcc gttcgtctgt atcagggagc
     2521 gatcaggaga agggtgtgtc caagacagag caatggatgg ctgtcgtcgt cggtgtcacc
     2581 attcccgctt cgctgctggt tgctgcgctc gcaggatggg tcggtctggt catcgtcgga
     2641 gtcgtcctga tcctgatgtg gcgcctgtcg ccaggtctct ggcgaatatt tggaatcggg
     2701 ctgctcggtg gagcgatagc cggcgtcctg atactcggac ctggattccg cctggcaatg
     2761 agggtcgtcg ccatcgtcga cgtccgccgg gtcgaattct ctgtcgccgg cacgatgttc
     2821 atcatcgtgc tcttaggtgt gatcgtggga ggactcggcg gcgtgttttc cgcaatgctg
     2881 aggaagggcc tcgggtggtc gagcctcgtc atgtccttgc tgatgacggt ggttctggtt
     2941 gccctcctcg tgatcgatac cggtttgcgc gacgagttgg tcacccttgg tgccgggccc
     3001 tggctcaaca tcccgatgtt cgccgcggtc tgcttcggct attcgctatt tgccaacagg
     3061 ctgatcgacc gcttcgagcg caagaagtcc cgtccagcgg ccagggagcc ggtcgaggtg
     3121 ccaacatgac tcgatacatg agagtcggac tgtcttgagc tggtgggcaa tcgagctgat
     3181 caccaacgtc atcctggtgg gtctagtcgt gttccttggt ccgctgatca agagattcgg
     3241 ccgcagttat gccggagacg tctttcgggg gaaccctcgc accggcaaga gctatctggt
     3301 cctgatggac gtcgcctatt acctgatctt cttcgccttg attctgttca ccgtcagctt
     3361 ccaggatcaa tggaacgacg tgggagagca actcgagagc gaattgagaa gggttggtgg
     3421 aatgctgctg ctcatggggg tcctccacgg gctgaacgtc ttgaccttgc ccctcgtcgg
     3481 acgtctgctc ggtctgggtc ggcagctcga ggaggacact ccgccacccg tgacctgacc
     3541 ttgtactggg ctgaagctct cgacaacacg gtgatctgga ccgcggagac gctggaccgg
     3601 cgcgccgtca tggaaagcct ctacaaacag gaggctccct ccctggtacg cctcgctcga
     3661 ctcttcaccg acgaccgcac cgccgctgag gacttggttc aggaagggtt catccgtctc
     3721 ttccactcgg cacaccggat cgacgatccg ggcaaggcgg cggcctatct gcgttccata
     3781 gttctgaacc tggcgcgcga tcacaatcgg cggggcctgc tctccctccg tcatcaagac
     3841 gccctgaccg agatcgcccc gcccgagcag cccgaggagc aggtcgtccg gagtgagagc
     3901 gaggcccagg ttctcgatgc cctacggtcg ctctcggccc gtcagcgtga ctgccttgtg
     3961 ctgcggttct acatggatct ctcggagaag gagatcgctt ccacgctccg gatctcaccc
     4021 aactcggtga agacccactg ccaacggggg atggccgctc tcgcctcgat tctgggggag
     4081 gagtcatgac tctcgagcag agattgatcg agacattgca ccgggcagat gacttcgagc
     4141 ccagccctga cctgttcgcc agggtcgagc gcagtctcga agaggaccga cgccaccggc
     4201 gccgcatcac ttgggtggcc gcgggcaccc tggcgggggc cgtggcagca agtctcttgc
     4261 ttcgggcgac gatctcgcac tccgcccttg gagaattgat cgcccctggt tgggtgctcg
     4321 agctgattgt caccctcgcc aaggtcgcac tgatcctggt tcttggcccc aacctgcgac
     4381 gtctaggcct gccactcatc gccgacgtgt tccgtctcga cccgccaact ggaaagcgat
     4441 tcctcaagct gttggacatc gccttctatc tgttcctgtt cgggcagatc ctgttggagg
     4501 cgtatcccca gctctgggat caagaagtgc tcctcggcga ggatgggtgg ttgtggaccg
     4561 gctcgatggc gatattcatg gtgatgatgg gcctcgggca tatcgcgacc atcgccgccc
     4621 ttccggtgat tggccttttc cattcgtccc tggtcagacg gaacagaagg tgggaagccg
     4681 gatctggtgc gccgcctcgt tcagagggcg ccgagcaagc ggagagggtg gtgcggctca
     4741 tcgtttgggg cattgctggt ttggcgctgc tctcggtcct catagcagtg ggtgtcctgt
     4801 tcgtgggtgg cgtggtcgac gcctgatcga agcaaccggc tctcagccgg aggccgttgg
     4861 cgctgccaga ctgtcgtcac tcgaaaggag ctttgtgacg tggcttggtg agccgggtgt
     4921 gagtagtggt ctatagccgt gacctgggtg ggcctggtgc ccgggcccgg gtcgccactc
     4981 ctaagccgaa ccgcagcctc taccggcgat tcctcaagga tgtcgccgac gcggggctgc
     5041 ccagcggtcc cggtccccag gagccggtcg gcgaggacga tctcgccagc ttgccggcga
     5101 cggcgcggcg ctatatggag ttcatgggtg tccccggcag accgagagac tggtcgcttc
     5161 gagcccgctt tgtcggacgt tttcggcttc gaccccatgc cgactggttg ccggccgagg
     5221 cctggcagta caacaccgcc ctcgaagtcg gtcgggtgta cacgatgcgg atcaggttcg
     5281 ccagtgtgct tccgatgaca ggcaaagaca cttatttgaa gggccacggg cagatgctcg
     5341 gcaagctggt cggtctgttc acggtggcag aagggcaggg ccctgagttc gacatcgggg
     5401 agctaaccac ctacctcaat gacgcgattc tccttgcccc ctcgttcctt ctcgggccaa
     5461 acacgacctg ggccgaagtc gatgacgaga gtttcgaggt cacgctcgcc gactcgggcc
     5521 gacatgtcac ggctcgggtc ttcgttgacg agcgaggtgc accgaccgac ttcagcacca
     5581 ccgaccgctt cgccgacctg cccggaggtt tggtgcgtgc gaaatggacg actccgactc
     5641 cggtctggct cagcgggtcg gggcgcccgg ttccgggacc gatgtcagcg gtctggcatt
     5701 tggcagacgg ccctctgccc tatatcgagg gccgtctcat cccggagagc atcaattaca
     5761 acgtggcgcc agaggcctga gtgcacaacc tgtcaacacc aatgacggtg ctggtagcgg
     5821 tacttgaacc agggctcggc ggtggcgctg gcgctgttgc ctgaccggtc ggttccggtc
     5881 accgtcaaca agaaggcggg agccttgatg aagaggacgc cgtgggcccg ataactcgag
     5941 gcaagcccat cggtcttgac cagatacacc ttctgaccgt cgcgcacctc gatcccgttg
     6001 atcgacccga gacatgaggg acgcctgtcg acccgatccc agcaggagaa gtcgaccttg
     6061 aaccagccac gaccatgatc ggtccagaag cggtcgaagt cggcgtggac cttcggggct
     6121 ttgacatcct taccgggact cggcggcaac gttggcaggg ttgggggtgg cacgtacagg
     6181 gtggtcgtgg tggtcgtcga cgtagtcgag gtggtcgagg tcgttgtggt tgtcgtcgaa
     6241 gtgcttgtgg tgctcgtggt cgaggtactg gttgtcgtcg tggtgggtgc agttgcgacg
     6301 tatgagcccc tcgggaaggg actcgttgct gtggcgttgg tcagagttgt tcccaccagt
     6361 gcgttgaccc cgctgaagtt ccagctggac aggtcaaagg tggatccgtc cgctccggtg
     6421 cccagcttcc gataggccca tccgttggtg tactcccacg gttgcccggt tccgtcgaca
     6481 tttatcacgc cgaagacatc gacgaccact ccgttctgga acagctcgat ggcgtcgtca
     6541 ccgttcacat ttattgccgt ggtcatgaag tccggggcga atccgaagaa gctggcgaac
     6601 tgggtgccct cgagtgagac gtagatgaag tctcctgcgg aggcggggcc gctggggaat
     6661 gtgaattcct gaccgtcggt gccgccgcca ttgttggccg acccaaaccc gtatacaccc
     6721 aggttgggga tgttgtttac gactaggaat tcaacggcct tcgggttacc gccagctcgg
     6781 ggtccttgga tgaccccgga aatgaccaag tcacccgtgg tggccgccgc aggggcgata
     6841 ccaatcggga tcaaggtgct cagcatggcg aagaccaaaa gcaggccgag tctggaacgt
     6901 gacaagtgct gctcctcgtg ctgttttcgc cgatcccggg ggtcacattg ggaaccggct
     6961 ggttgtcaat agcggggagc accaccgacg acctacattc caccctggtt tttccacaag
     7021 gcaagaccca ttctccccca gcgaacaaag tgagcctgga agggagtacc gccgatggcg
     7081 gggaggggtt tacccggaaa cggttgagac cgacttcttg ccgagccgtc ttctctcggg
     7141 ctcgctgagg aaccgcttgc ggacccggat cgaggacgga gtgacttcga ccagctcatc
     7201 gtcggcgatc cattcgatgg cggtctccag cgtcatctca cggggtggcc ggagattgat
     7261 cgcctcgtcg gaagcatggg ttcggatatt ggtgagttgt tttggccggg ccgcgttgac
     7321 caccatctca tcgggtcgcg acgcctcgcc gatgatcatg ccttcgtagg tctcctcacc
     7381 ggcaccgatg aacaattcgc cacgcttctg caggttgtcg agggcgaatc cggtgctggt
     7441 ccccttgcga tcggaaacca tggcgcctcc gatcctgtgg gggagctcgc caacccatgg
     7501 catccatcct tcatgatgtt gatggatcag ggcggtgccg cgggtggtcg tcatcaggag
     7561 cgaccggaag ccgaggagcc cgcgggccgg agcggtgaag gtgatgacgg tgcgcccggg
     7621 atccccgggc tgcaactcgc ggatggttcc cttccggggt gctgcggcct gtgtgaccgc
     7681 ccccacgtac tcgtcgggga catcgaccac ggctcgctcc accggctcgt gggggctgct
     7741 gtcgatcatc cgaaccacga cctccggtct actcacctgg agctcgaagc cctctctgcg
     7801 catcgactcg atgagcacgg cgagttgcaa ctctccccga cccgccactt cgacgatctc
     7861 aggcgatgac gtcggtccga gtcggatcga aacatttccg agaacctccc tcttgagccg
     7921 gtcct
//
LOCUS       DBNHXU010000334         1839 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2105569, whole genome shotgun sequence.
ACCESSION   DBNHXU010000334 DBNHXU010000000
VERSION     DBNHXU010000334.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1839)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1839
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2105569"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            78..581
                     /locus_tag="AC161X_08265"
     CDS             78..581
                     /locus_tag="AC161X_08265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019587516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HIR4989584.1"
                     /translation="MNVSMTVNGREVSADVETRTLLVHLIRENLNLTGTHVGCETGYC
                     GACTVHVDGKPVKSCTMLAVQAEGANIMTIEGLANNGELHPVQEGFWERHGLQCGFCT
                     PGMIMAAAALLAENDNPSETEIRHGIEGNLCRCTGYHNIVRAVEYAGARMRGEEPAAP
                     DWEEVTS"
     gene            581..>1839
                     /locus_tag="AC161X_08270"
     CDS             581..>1839
                     /locus_tag="AC161X_08270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR4989585.1"
                     /translation="MASTSVMGGSVRRREDPALIQGYGEYVDDLTPTGMLHVAFLRST
                     VARAKITKLDTTAAKEMKGVIAIYTHDDVAHLGPLLAQVPIGKLRPLLAAGEVNHVGE
                     AVAMVVAEDRYQAQDAVEAIEVDYDTLPAIIDLKKAASDEVKVHDELDSNVLHTWTYH
                     GYWAALGLEDQTPQVDAAMERDDAVVVSLEMINQRLIPVAIEPRSVLAEYNPGYSCFT
                     IHSSTQVPHALGGAISKTFGMKSPSVRVIAPEVGGGFGSKLNVYNDEILVSFAAKELG
                     RPVKWTETRREAAGSTIHGRGWIGTATLVGTKDGQFLGYKLDAIADMGAYTQNFTVAI
                     PLLGLWIASGQYKMPVSWTVACVVTNTMTTDAYRGAGRPEAIYYLERIIDTYAREIGM
                     DPLEVRKKNFWQPDEFPAQMGCGFSLDS"
BASE COUNT          360 a          616 c          556 g          307 t
ORIGIN      
        1 cgaatccggc cgtcacgacc tagcccgttc tgataggttc cccgccgtcc catcgaacaa
       61 ggccgaaagg ggtcttcatg aatgtgtcga tgaccgtcaa cgggcgggag gtgtcggccg
      121 acgtagagac caggacgctt ctcgtccatc tcatccggga gaacctcaac ctcaccggaa
      181 ctcacgtcgg ctgtgagacc ggctattgcg gcgcctgtac cgttcacgtc gacggcaagc
      241 cggtgaaatc atgcaccatg ctcgccgtgc aggccgaagg tgccaacatc atgaccatcg
      301 aggggctcgc caacaacggc gagcttcatc cggttcagga gggtttttgg gagcgccacg
      361 gtctgcaatg tggcttctgc acccccggga tgatcatggc ggcggccgcc ctgctcgctg
      421 agaacgacaa cccctcggaa accgagatcc ggcacggcat cgaaggcaac ctctgccgtt
      481 gcacgggcta tcacaacatc gtccgagccg tcgagtacgc cggggccagg atgcgtggcg
      541 aagagccggc cgcaccggat tgggaggagg tgacctcctg atggctagca ccagcgtcat
      601 gggcggctcc gtccgccgtc gcgaagatcc cgccctcatc cagggctatg gggaatacgt
      661 agacgacctc actcccaccg gcatgctgca tgtcgctttc ctcagatcga cggtggcgag
      721 agccaagatc accaagctcg acaccactgc cgccaaggag atgaagggtg tcatcgccat
      781 ctacacccac gacgacgtgg cccacctcgg tccgctgctg gcgcaggtgc cgatcgggaa
      841 gctgcgtcct ttgctcgccg ccggcgaggt caaccacgtc ggtgaggcgg tggcaatggt
      901 cgtcgccgag gaccgctatc aggcccagga tgccgtcgag gcgatcgaag tcgactacga
      961 cacactcccg gccatcatcg acctcaagaa ggccgcctca gacgaggtga aggtccacga
     1021 cgagctcgac tccaacgtcc tccacacctg gacctaccac ggctactggg ccgcgctcgg
     1081 tctcgaggac caaacaccac aagtggatgc cgccatggag cgtgatgacg ccgtggtcgt
     1141 ctccttggag atgatcaacc agcgcctgat cccggtggcc atcgagcccc ggtcggtgct
     1201 ggccgagtac aaccccggat acagctgctt caccattcac agctccaccc aggtccctca
     1261 cgccctcgga ggcgcgattt cgaagacctt cgggatgaag tccccctcgg ttcgggtgat
     1321 cgcacccgaa gttggtggtg gtttcggcag caagctcaac gtctacaacg acgagatcct
     1381 ggtgtcattc gccgccaagg agctgggacg ccccgtgaag tggaccgaga cccgtcgcga
     1441 ggcggcaggc tcgaccatcc atggccgggg ctggatcggc accgccaccc tcgtggggac
     1501 caaggacggc cagttcctcg ggtacaagct ggatgccatc gccgacatgg gcgcctacac
     1561 ccagaacttc accgtcgcca tccccctcct cgggctctgg atcgcctccg ggcaatacaa
     1621 gatgccggtc tcgtggaccg tggcctgtgt ggtgaccaac accatgacca ccgacgccta
     1681 ccggggcgcc ggccgtcccg aggcgatcta ctacctggaa cggatcatcg acacctacgc
     1741 acgtgagatc gggatggatc ccctcgaagt gcggaagaag aacttctggc agcccgacga
     1801 gttcccggct cagatgggct gcggattctc tctcgactc
//
LOCUS       DBNHXU010000335         6982 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2305657, whole genome shotgun sequence.
ACCESSION   DBNHXU010000335 DBNHXU010000000
VERSION     DBNHXU010000335.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6982)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6982
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2305657"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..486
                     /locus_tag="AC161X_08275"
     CDS             64..486
                     /locus_tag="AC161X_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HIR4989586.1"
                     /translation="MDNLSIFMEHTPVSALQRTPQPEEHFTGKAWMETLSQGDDRVLN
                     AISVTFEAGARTHWHSHPEGQVLYVVSGYGRGGTETGDFFEFAAGDVLYSKPDELHWH
                     GASPGSPMSHLSLTTGGATQWKPRPVSDEEYENSPPGG"
     gene            518..1801
                     /locus_tag="AC161X_08280"
     CDS             518..1801
                     /locus_tag="AC161X_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011690399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HIR4989587.1"
                     /translation="MKDHEFGFATRAVHAGQRPDPETGARAMPIYATTSFVFDDVQHA
                     ADLFALQTYGNIYTRIGNPTTAAFEERMASLEGGLGAVATASGQAAQLIAILTLCQQG
                     DEVVASRSLYGGTYTQFDVTLRRMGIDVTFVDFGDIDAVQAAITDKTKLLYGETVGNP
                     GADILDIETIARLAHDNGVPLVVDNTFATPYLCRPIEYGADIVVHSATKFLGGHGAAI
                     AGVIVESGRFPFDNGKFPTLVEPSPAYHDLRFWENFREYAYLTRARVELLRDIGASLS
                     PFNAFILLLGVETLPLRMQRHVENARAVAEFLRAREEVAWVNFPIFEDNPWTELAMKY
                     LPKGPGAVFTFGLRGGLTAGIRFIETVELASHLANVGDAKTLVIHPASTTHQQVPVAE
                     RERAGVGDDMIRISVGIEDIEDIVWDLDRALVASS"
     gene            1798..2277
                     /locus_tag="AC161X_08285"
     CDS             1798..2277
                     /locus_tag="AC161X_08285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018776128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR4989588.1"
                     /translation="MTVADPEARDRLRIILGARSVALVGVSANPIRSSNFVASYLVRT
                     DFVTYPVNPMYDEVLGLKSYPSLRDLPEPPDIVDVFRKHSEIPGVVDEAIEVGAKVVW
                     FQLGLRHEEAARKAVDAGLKVVQDRCLKIEHARFAGGLHLGGFDTGVITSKRRRPIG"
     gene            2336..3676
                     /gene="xylA"
                     /locus_tag="AC161X_08290"
     CDS             2336..3676
                     /gene="xylA"
                     /locus_tag="AC161X_08290"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010556356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HIR4989589.1"
                     /translation="MGNDFFIEVTGPIPFGGLGSQEPLSFKVYEPDRLVLGKRMEDQL
                     RIAVCLWHSFNWPGSDVFGMGTFDRPWLSPDVDPMTAAANKLEAAFEFLSKLGVPYFC
                     FHDRDVAPEGSSFKETRANLETVIDQIEGHMSGTGLKLLWGTANLFSHPRYAAGAATN
                     PDPDVFAYAAAQVKVMLEATHRLGGDNYVLWGGREGYETLLNTDLAREETQFARFLEM
                     VAEHKRQIGFKGTLLIEPKPQEPTKHQYDYDCATVHGFLVRHGLDGEYKVNIEANHAT
                     LAGHSFHHEVAYATANGIFGSIDANRGDYQNGWDTDQFPNSVDELSLALYEIINSGGF
                     TTGGFNFDAKLRRQSMHRTDLFHGHIGGIDTLARALLVAADMVESKTLEGPRTDRYAG
                     WAKGIGASILAGEVTLDGLEDKVASGEIDPKPTSGRQELLENEVNQRIWATKKD"
     gene            complement(3681..4388)
                     /locus_tag="AC161X_08295"
     CDS             complement(3681..4388)
                     /locus_tag="AC161X_08295"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012706183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4989590.1"
                     /translation="MINLEGRVALITGASKGIGAAVAHGLVAQGAKVALASRSGDDLG
                     IDGAVAQPCDVRDPAALDSIVAETVERFGGLDILVANAGVGAYGSFLDLDPEHLEEMI
                     DVNLKGTLYAVRAALPHLLKSNAADIVTLASEAGRRGLPYEAVYCASKFGQVGFTRAM
                     DHELREKGVRCTNVCPGGVATDFAMGRGRTPNMPELDGMMTSEDVAEMVLFVLTRPRN
                     HRILEVAMRPVTETSWG"
     gene            complement(4413..5207)
                     /locus_tag="AC161X_08300"
     CDS             complement(4413..5207)
                     /locus_tag="AC161X_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007769764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4989591.1"
                     /translation="MTTPSTSKPPLVEMRNMSVSFGGVHAVKDVTITLREGEVLGVVG
                     GNGAGKSTLIKVLAGAQKADSGEIFIDGEPVTIHSPRDAKNLGIETIYQTLALADNID
                     APGNMFLGRELTTRWNTLDDSSMEAATREIMGRLNPKFQNFKTPVASLSGGQRQSVAI
                     ARAVHFNARILIMDEPTAALGPAETEQVRELIRQLQREGIGIIMISHDIHDVFDLSDR
                     ISVMLQGKLVGTVNRGECTMDEILAMIIIGKLPDEVTTKELEQLHG"
     gene            complement(5204..6526)
                     /locus_tag="AC161X_08305"
     CDS             complement(5204..6526)
                     /locus_tag="AC161X_08305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007533819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HIR4989592.1"
                     /translation="MSVVEAAPSQMETLEPGPRRSVGSFLRDNEIDTRMVGMTLILLV
                     IWVLFHILSGGTFLTPRNLWNLSVQTSSIAIMATGMVLVIVSRNIDLSVGSMVGVVGM
                     VMAILQFEWLPPVLGLGNQWIWIITLVAGLALGALIGAFQGSLIAYLGVPSFIVTLGG
                     LLVWRGFAWLTASGRTLAPMDATFQLLGGGSRGSLGETGSWIVGGVACVGIVAFLVMG
                     RRRRRKFGFHVRPMWLDAALGVIGCVVVLAAVWVTNSYYLPENLARAVAEERGIPWPE
                     GGLQIPLGIPYPVLILIGVTLVMSFVANRLRFGRYIFSIGGNPEAAVLAGIKTKRTIV
                     RTFMTMGILVGISAAVSIARLNAAVSGLGTLNELYVIAAAVVGGASLAGGIGTIPGAI
                     IGALVMQSLQSGMVSLKVSAPVQDVVLGIILVTAVAIDTTFRRRAAQR"
     gene            complement(6620..>6982)
                     /locus_tag="AC161X_08310"
     CDS             complement(6620..>6982)
                     /locus_tag="AC161X_08310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-xylose ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR4989593.1"
                     /translation="LAAQGLSIPLSGQDADVAALNRVALGTQSVSVWKDARELGKAAG
                     TAAVALAANPSLADVEGTAEFVYTNKTSGVATTLVSVLLTPIPITQENLNLVVDANWI
                     TADELCAGVTAGSVAACP"
BASE COUNT         1397 a         2218 c         2126 g         1241 t
ORIGIN      
        1 ctgtaccgga ttcgcaccag gaccaccgga gccccccgta cgacgcatta gggcctgaca
       61 tccatggata acctctccat cttcatggag cacacccctg tcagcgccct gcaacggaca
      121 ccgcagcccg aggagcactt caccggcaag gcctggatgg agacgctgtc ccaaggtgac
      181 gatcgcgtgc tgaatgcgat cagcgtcacg ttcgaggctg gagcgcggac ccactggcac
      241 tcccaccctg aaggccaggt cctctatgtc gtgtccggct acgggcgagg agggactgag
      301 accggtgatt tcttcgagtt cgcggccggc gacgtgctct actccaagcc ggacgagctt
      361 cattggcacg gggccagccc gggctcgccc atgagccacc tctcattgac taccggtggc
      421 gcgacgcagt ggaagccgcg gccggtgtcc gacgaggaat acgagaacag ccctccgggc
      481 ggctgagccc agcgagttgg atgccaacat aggaccgatg aaagaccacg agttcggttt
      541 cgccactcga gccgtgcacg ccggccagcg ccctgatccc gagaccgggg cccgggccat
      601 gcccatatac gcgaccacgt cgtttgtatt cgacgacgtc cagcatgccg ccgacctgtt
      661 cgccttgcag acctacggga acatctacac ccgcatcggc aacccgacca cggctgcgtt
      721 cgaggagcgg atggcgtcac tcgagggagg tttgggggcg gtcgccacgg cctcgggaca
      781 ggctgcccag ctgatcgcga tccttaccct gtgccagcag ggtgacgaag tggtcgcctc
      841 gcgatctcta tacggcggga cttacaccca gttcgacgtg actctgcgga gaatgggcat
      901 cgacgtcacc ttcgtcgact tcggagacat cgatgcggtt caggccgcca tcaccgacaa
      961 gacgaaactg ctctatgggg agaccgtcgg caacccaggc gccgacatcc tcgacatcga
     1021 gacgatcgcc cgtctggccc acgacaacgg cgttccattg gtcgtggaca acacctttgc
     1081 cactccctac ctatgccgac ccatcgagta cggcgcagac atcgtcgtgc actccgcaac
     1141 caagttcctc ggcggccatg gggccgcgat cgccggagtg atcgtggaga gtggtcgctt
     1201 tccgttcgac aacgggaagt tccccaccct cgtggagccc tcgccggcct atcacgacct
     1261 ccggttctgg gagaacttcc gtgagtacgc ctacctgaca cgagcgaggg tggagctcct
     1321 ccgcgacatt ggcgcctcgt tgtcaccttt caacgctttc attctcctgc tcggcgttga
     1381 aacccttccg cttcgcatgc agagacacgt cgaaaacgcg cgggcagtgg ccgaattcct
     1441 ccgagcacgc gaggaggtgg catgggtcaa cttcccaatc ttcgaggaca acccgtggac
     1501 ggagctcgcc atgaaatacc tgccgaaagg gccgggggct gttttcacgt tcggcctccg
     1561 gggcggcctg acggcaggga ttcgcttcat cgagaccgtt gagttggcat cgcatctcgc
     1621 caacgtgggt gatgccaaga ccctggtgat ccatccagcg tcgaccacgc atcagcaggt
     1681 gcccgtcgcc gaacgggagc gggccggtgt gggtgatgac atgatcagga tctcggtcgg
     1741 catcgaggac atcgaagaca tcgtctggga cctcgatcgg gccctggtgg cgtcgtcatg
     1801 accgtggccg acccggaagc gagggaccgg ctccgcatca tcctcggcgc tcgcagcgtg
     1861 gcattggttg gcgtttcggc caatcccatt cgatcctcga acttcgtcgc cagctacttg
     1921 gtccgcaccg actttgtcac ctatccggtc aatccgatgt acgacgaggt gcttggcctc
     1981 aagtcctacc cgtccctgcg ggaccttccc gagccacccg acatcgtcga tgtctttcgg
     2041 aagcactctg agatacctgg agtagtggac gaggcgatcg aggtcggagc caaggtcgtg
     2101 tggttccaac tcgggttgcg tcacgaagag gcggcgcgaa aggcggtcga cgcaggtctc
     2161 aaggtggtcc aggaccggtg tctcaaaatc gagcatgcgc gattcgccgg tggtctccat
     2221 cttggagggt tcgacaccgg cgtcatcacg tccaagaggc gacgccccat cgggtaaggg
     2281 tgaataccct gagtgatagg ctcgcccagc ttccctcgag ttcgaaaggc atggcgtggg
     2341 caacgacttc ttcatagagg tgaccggacc gattccgttc ggagggctcg ggtcgcaaga
     2401 gccgctttcc ttcaaggtct acgagcccga caggctggtc ctgggaaaga ggatggaaga
     2461 ccagctccgg atcgcggttt gcctctggca ctcgttcaac tggcccggat ccgacgtctt
     2521 cgggatgggg accttcgacc gcccctggtt gagccctgac gtcgacccga tgacggcagc
     2581 agccaacaag ctcgaagccg ccttcgaatt cctctccaag ctcggggtcc catatttctg
     2641 cttccacgac cgagacgtcg cccccgaggg atcctcgttc aaagagaccc gagcgaatct
     2701 ggagacagtg atcgaccaga tcgagggtca catgtcgggg accgggctca agctgctgtg
     2761 gggaacggca aaccttttca gccatcctcg atacgccgcg ggagcggcca ccaatcccga
     2821 ccccgatgtc ttcgcctacg ccgcggccca ggtgaaggtg atgctcgagg cgacccaccg
     2881 attgggcggt gacaactatg tcctctgggg tggaagagag ggatacgaga ccctcctgaa
     2941 cacagacctc gctcgtgagg aaacccagtt cgcccgattc ctggagatgg ttgcggaaca
     3001 caagcgccag atcgggttca agggcaccct gctgatcgag cccaagcccc aggagccgac
     3061 caagcaccag tacgactatg actgcgcgac agtccacggc ttcctggtcc gccacgggct
     3121 cgacggcgag tacaaggtca acatcgaggc caaccacgcc actctggccg gtcacagctt
     3181 tcaccatgag gtggcctacg ccaccgccaa cggcatcttc ggcagcatcg acgccaaccg
     3241 cggcgactac caaaacggct gggacacgga ccagtttccc aactcggtcg acgagctctc
     3301 gctggcgctt tacgagatca tcaacagcgg cggcttcacc acgggagggt tcaacttcga
     3361 cgccaagctc cgacgtcaga gcatgcaccg aaccgacttg ttccatggtc acatcggagg
     3421 gattgacacc ttggcccgag cccttctcgt ggcagccgac atggtcgagt ccaagactct
     3481 cgaagggccg cgaacggatc gctatgccgg gtgggccaag gggattggcg cttcgatact
     3541 cgccggtgag gtcacactcg atggcctcga ggacaaggtc gcctccgggg agatcgaccc
     3601 caaaccgacg tcgggacgcc aggagctcct cgagaacgag gtgaatcagc ggatctgggc
     3661 gaccaagaag gactgaggtc tcagccccag gaggtctcgg tcaccgggcg cattgccacc
     3721 tccaaaatgc ggtggttgcg gggacgggtc aggacgaaga gcaccatctc ggccacgtcc
     3781 tccgatgtca tcatcccatc cagttccggc atattcggag tgcggcccct gcccatcgca
     3841 aagtcggtcg ccacaccacc ggggcagaca ttggtgcaac gcacgccctt ctcacgcagc
     3901 tcgtggtcca tggccctggt gaaaccgacc tgcccgaatt tggaagcgca gtacaccgcc
     3961 tcgtagggaa ggccacgccg accggcctcg ctggcgagtg tcacgatgtc ggccgcattg
     4021 ctcttcagaa ggtgagggag ggcggcgcgc accgcgtaaa gggtgccctt gagattgacg
     4081 tcgatcatct cttctagatg ctcggggtcc aggtcgagga acgacccgta ggcgccaaca
     4141 ccggcgttgg cgaccaggat gtcgagcccg ccaaagcgtt cgacggtctc ggccactatt
     4201 gaatcgaggg cggcgggatc acgcacatcg cagggttgtg cgaccgcgcc gtcaattccc
     4261 agatcgtcac cacttctgga agcgagagcc accttggctc cttgagcgac caggccatgc
     4321 gccaccgcgg cgccaatgcc cttgctggct ccggtgatga gcgcgactct tccttcaagg
     4381 ttgatcatgg tgtttccttc cgccagcagc attcagccgt ggagttgttc cagctcttta
     4441 gtggtgacct catcgggcag cttgccaatg atgatcatgg ccaggatctc gtccatggtg
     4501 cactctccgc ggttcaccgt tcccaccagc ttgccctgca gcatgacact gatccggtcg
     4561 gacagatcga acacgtcgtg gatgtcgtgg ctgatcatga tgatcccgat cccctcgcgt
     4621 tgaagctgac ggatcaactc ccgtacctgc tcggtctcag ccgggcccag agccgcggtt
     4681 ggctcgtcca tgatcagaat cctggcgttg aaatgaacgg cccgggcgat tgcgaccgac
     4741 tggcgctggc caccggacaa agaggccaca ggcgtcttga agttctggaa cttggggttg
     4801 aggcgcccca tgatctctcg ggtagcagcc tccatcgatg agtcgtcgag cgtgttccag
     4861 cgagtcgtca actctctccc gaggaacatg ttgcccggag cgtcgatgtt gtcggcgagg
     4921 gccagtgtct gatagatggt ctcgatcccc agattcttgg cgtcgcgtgg actatggatg
     4981 gtcaccggct caccgtcgat gaagatctca ccggagtccg ccttctgggc acctgccaaa
     5041 accttgatca gggtcgactt gccggctccg ttgcccccga ctactccgag cacttcacct
     5101 tctcggagag tgatggtgac atcctttacc gcatgcacac caccgaagga gacactcatg
     5161 ttcctcatct cgacgagtgg tggtttggaa gtagaggggg ttgtcatcgt tgggccgccc
     5221 ttcgccggaa ggttgtgtcg attgcgactg cggtcaccag gatgatcccg agtaccacgt
     5281 cctgcacggg agcactgacc ttgagagaaa ccatgccgga ctggagggat tgcatgacca
     5341 gggctccgat aatggctcct ggaatggtcc cgatgccacc agccaaggag gcacccccga
     5401 cgacggccgc tgcaatcaca taaagctcgt tcaacgtgcc gagaccgctc accgccgcat
     5461 tgaggcgggc gattgagacc gcggctgaaa tgccgaccag gattcccatg gtcatgaagg
     5521 tcctgacgat ggtgcgcttg gtcttgattc cagcgaggac ggcagcctcg ggattgcccc
     5581 caatcgagaa gatgtagcgc ccgaagcgga gccggttggc gacgaagctc atgaccaagg
     5641 tgactccgat gaggatcagg accggataag gaatgccaag cgggatctgg agaccaccct
     5701 cgggccaggg gatgcccctt tcttcagcga ccgcccgagc gaggttctcg ggcaggtagt
     5761 agctgttggt gacccacacc gcggcgagca ccaccacaca gccgatcaca ccgagagcag
     5821 catccagcca catcggcctg acgtggaagc cgaacttgcg acgacggcgg cgtcccatga
     5881 cgagaaaggc gacgatcccg acacacgcca ccccgccaac gatccaactc ccggtttcac
     5941 cgagcgaccc ccgagaccca cccccaagga gttggaaggt ggcgtccatg ggggcaagag
     6001 tccggccgct ggcggtcagc caggcaaacc cacgccatac cagcaatcca cccagggtca
     6061 cgatgaacga cggaacgccg agataggcga tgagcgaacc ctggaatgcg ccgatcagcg
     6121 ctccgagagc caatccggcg acgagggtga tgatccagat ccactgattg ccgagaccca
     6181 acacgggcgg tagccactcg aattggagga tggccatgac catgccaacc acgccgacca
     6241 tcgaaccaac ggagaggtcg atgttgcggg agacgattac cagcaccatc ccggttgcca
     6301 tgatcgcaat cgaagaggtc tgcaccgaga gattccacag gttgcgaggg gtgaggaagg
     6361 ttccgcccga caagatgtgg aagagcaccc agatcaccag gaggatgagg gtcatcccca
     6421 ccatgcgggt gtcgatctcg ttgtcccgca ggaaggagcc aactgaccgg cgtggacctg
     6481 gttctaaggt ctccatctgg gacggggcag cctctaccac gctcatgaac tcaccacttt
     6541 agagaaggcg gtcgcattgc gcccccggag aaatccgggg gcgcgaatgc actatcgatt
     6601 tcgcttcagc tgtcgacgat cacgggcaag cagcaacgct gcctgcggtg acgccggcac
     6661 agagctcgtc agccgtgatc cagttggcgt cgaccaccag gttcaggttc tcctgagtga
     6721 tcgggatcgg ggtcagcaga acgctgacca gggttgttgc tacgcctgag gtcttgttcg
     6781 tgtaaacgaa ctcggccgtt ccttcgacat cggcaagcga cgggttcgcg gccaaagcaa
     6841 cggcagccgt accggcagcc ttgcccagct cacgagcgtc cttccacacc gacaccgact
     6901 gcgttcccaa cgccacccgg ttgagggccg cgacgtctgc gtcctgaccc gacaacggga
     6961 tgctgaggcc ctgggccgcg ag
//
LOCUS       DBNHXU010000336         1630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1513309, whole genome shotgun sequence.
ACCESSION   DBNHXU010000336 DBNHXU010000000
VERSION     DBNHXU010000336.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1513309"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(184..1194)
                     /locus_tag="AC161X_08315"
     CDS             complement(184..1194)
                     /locus_tag="AC161X_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010042747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989594.1"
                     /translation="MHPGVTPLVEVEDLHVNFGPARAVDGASLQVGPGEVVALVGESG
                     SGKTTLIRAIQGLQTPTSGSIKVEGKPIDAKTKKAVQTIFQNPAVQMVFQDPTGALNP
                     RQTLYEIVAEGLRIHGQQGGEEAKVAQALSRAGLRPPERFFQMYPHEISGGQRQRVVI
                     AGAMVMEPRLLLADEPVASLDASIRGEILALMRHLVDDTDLAILVVTHDLGLAWNIAD
                     RVAVMYLGRIVETGLTEDILGDPKHPYTRALLSVVPETKEMEQQILIGEIPDATRIPA
                     GCRFHPRCPLLQSGTLSPEVAARCTTDDPGPVAVGAVAAAACWAVVSPPRSGATLGRA
                     PA"
     gene            complement(1179..>1630)
                     /locus_tag="AC161X_08320"
     CDS             complement(1179..>1630)
                     /locus_tag="AC161X_08320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003951198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HIR4989595.1"
                     /translation="PLLLIADEPTTALDLMVQAQVLALLDDLKKERDLTLLFITHDLS
                     VLTYACDELAVMYAGRIVEKGPSFEVFAEASHPYTRALAAAFPTIGDQSSRMTPSGLG
                     GDPPTPGNLPEGCPFHPRCPLAQDACRVEEPALRVTGEGRSAACILV"
BASE COUNT          291 a          523 c          525 g          291 t
ORIGIN      
        1 ggttggcctc gggctggcgg tggcaaggag tttggcccgg gtgatggatg gcgatctgac
       61 ttacgaatac gacggggagt cggtgttcaa gctggctctt ccgttggcct gactctctac
      121 cggccgactc ctcgcgccgg ccgagaagat ccagctgtcg cgccggcgcg ccccccaccc
      181 cggctacgcc ggtgctctcc ccaacgtcgc tccgctcctg gggggagaaa caactgccca
      241 gcaggcggcc gccgccaccg cccccactgc taccggcccc ggatcatccg tggtgcacct
      301 tgcggccacc tctggagaaa gggtgccgga ctggagcaac ggacatcggg gatgaaatcg
      361 acagcctgca gggatccggg tcgcgtccgg gatctcacca atcagaatct gttgctccat
      421 ctccttggtt tcgggtacca ccgagagcag ggcgcgggtg tagggatgtt tgggatcacc
      481 gaggatgtcc tcggtgaggc cagtctcgac gatccgaccc aggtacatga cagccacccg
      541 atcggcgatg ttccatgcga gaccgaggtc gtgggtcacg acgaggatcg ccagatcggt
      601 gtcgtcgacc agatgtcgca tcaatgccag gatctcgccc cggatcgagg cgtcgaggct
      661 ggctaccggc tcgtcggcca ggagcaagcg cggctccatg accatggccc ctgcgatgac
      721 gacgcgttgg cgctggcctc ccgatatctc atgtgggtac atctgaaaga agcgctcggg
      781 cggccggagg ccggcccggg agagcgcctg ggccaccttc gcctcctcgc ctccttgttg
      841 gccgtgaatg cggaggccct cggcgacgat ctcatacagg gtctgacgcg gattgagggc
      901 acctgtcgga tcctggaaca ccatctgcac tgccgggttc tgaaagatgg tctgaactgc
      961 cttcttggtc ttggcgtcga tcggcttgcc ttcgaccttg atgctgcccg aagttggggt
     1021 ctgcaatcct tgaatcgccc ggatcagagt cgtcttgcct gagcccgact ccccgaccag
     1081 ggcaacgact tctccgggcc cgacctggag agacgctccg tctacggccc gcgccggccc
     1141 aaagttcaca tgaaggtcct cgacctcgac gagaggggtc acaccaggat gcatgcggcg
     1201 ctcctgccct ccccagttac acgcaaagca ggctcctcga cacgacaggc gtcctgagcc
     1261 agtggacatc gagggtggaa cggacaacct tcagggagat tccccggggt gggggggtcg
     1321 cctccgagac ccgacggcgt catcctcgag ctctgatccc cgatggtcgg aaacgcagcc
     1381 gcgagcgccc tggtgtaagg gtgggaggcc tcggcgaaga cctcaaagct cgggcccttc
     1441 tcgacgatcc gccctgcgta catgactgcc aactcatcgc aggcataagt gagcaccgac
     1501 agatcatgcg tgatgaaaag cagagtgagg tcgcgctctt tcttgagatc gtccagaagc
     1561 gccagaacct gggcctgcac catgaggtcg agcgccgtgg tcggctcgtc agcgatcagc
     1621 agcagtggat
//
LOCUS       DBNHXU010000337         3042 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5481709, whole genome shotgun sequence.
ACCESSION   DBNHXU010000337 DBNHXU010000000
VERSION     DBNHXU010000337.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5481709"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="AC161X_08325"
     CDS             complement(<1..617)
                     /locus_tag="AC161X_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="13E12 repeat family protein"
                     /protein_id="HIR4989596.1"
                     /translation="MVGRSAVDYHESELVRLEGLKSQIAALQMRHLSHLDRAQVATAD
                     GSRSLSEWVAARLDLGHETAKALVRTMHRIEARPDLTEALAAGEATFDRVEALSRIQE
                     DVGLLQHLDVAGVRRTAALKALITPEEEFRSADERFFVIQPSLDESCWKLWGGLDGAS
                     GALVDKVLTEAADQLPELPDGTRGNESWRKATALVELCMSDNPPPS"
     gene            complement(700..1401)
                     /locus_tag="AC161X_08330"
     CDS             complement(700..1401)
                     /locus_tag="AC161X_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989597.1"
                     /translation="MTAEAEVATTEPKHQPLDWIDIVATIVMAFAALATAWSAFQSDQ
                     WGDTMSFSLNEAGAARTESTRSFTRAGQLSSIDVATFLAWLDAAKQENDSGEIDVSDG
                     YHPDPATLSGFLYARFRPEFKTAMDAWLATRPLSNPDAPPTPFAMEEYQVAQEIEAER
                     LLQEAQDKSALAHAADDNDDKYVISTIIFAGIFLFAGLSTKMRSLAGQYIMLGFSIVL
                     LIGGIVFLLTVPIQV"
     gene            complement(1407..2360)
                     /locus_tag="AC161X_08335"
     CDS             complement(1407..2360)
                     /locus_tag="AC161X_08335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012227376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit
                     alpha/FixB family protein"
                     /protein_id="HIR4989598.1"
                     /translation="MAAIWVFVEEEGGSPTTIGLELLAAGRQLGEVTAIYLGTGSDGA
                     FETLGEHGATSVLHLDAGSRLPAGPVTTALAARIADQAPALIIFGQAYTDRDVAGRLA
                     ARLGVGVLSNAIAVRLVDGGVETDHEILGGAQVATASMTQGPLLVLIRPKSFVAEAVG
                     GPMPAVEEIGLPDPARSEATVTESHVEEREGPQLGEAAVVVSGGRGLGSPEAYELVER
                     LGKKLGAATGATRAIVDAGWVPYAKQVGQTGKTVKPDVYIACGISGAMQHLVGMKDSK
                     TIIAINKDPDAPIFSIADLGVVGDVHKVLPKLIEALESREG"
     gene            complement(2362..>3042)
                     /locus_tag="AC161X_08340"
     CDS             complement(2362..>3042)
                     /locus_tag="AC161X_08340"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013200.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HIR4989599.1"
                     /translation="LRPDDQILDDTDRYGVEMGLQLAQASEGTVTLVCMGPAGNGQGI
                     RQALAMGADQAILIEDDTLRGSNALTTARVLAAAIQRDGFDLVITGTESTDGYSGVLP
                     QMLSELLDVPALTYATKVEAADGKVTIHRQTASGYDVVEASLPAVVAVTAGVVEPRYP
                     TFKGIMDAKKKTVESLSVADLGVERGPDQTVASITDAAERTAGQKIEDDGESHLAIVA
                     LLEQRKVI"
BASE COUNT          523 a          978 c          962 g          579 t
ORIGIN      
        1 gacggtggcg gattgtccga catacaaagc tcgaccagtg cggtggcttt tcgccacgac
       61 tcgttgccgc gggtgccgtc cgggagctcg ggaagctggt cagcagcttc ggtgagcacc
      121 ttgtccacca gggcccccga cgccccgtct aggcctcccc acagtttcca gcaggattcg
      181 tctagggagg gttggatcac gaagaagcgc tcgtcggcgc tccggaattc ctcctcgggc
      241 gtgatcagcg ccttcaaggc ggctgtccga cgaacaccgg caacatccag atgttggaga
      301 aggcccacat cctcttggat ccgggagagg gcctcgacac gatcgaaggt ggcctcgccg
      361 gcggccagag cctcagtgag gtcgggccga gcctcgattc ggtgcatggt ccggaccagc
      421 gcctttgcgg tctcatggcc caagtcgagt cgggccgcca cccactccga gagcgacctt
      481 gacccatccg ccgtcgctac ttgggcccga tcgaggtgac tcagatgtct catctgaagg
      541 gcggcgatct gtgatttcaa gccttccagt cggaccaatt ccgactcgtg gtagtcgact
      601 gcggatcttc caaccatgac ccgaacatac gttctggctg ggacagaaac aagagggttg
      661 tgacgctttt cgcgaaattt ctggcgggcc cgccctcact cagacctgga tcggcaccgt
      721 tagcaagaag acgatccccc cgatcaggag gacgatcgag aagccgagca tgatgtattg
      781 gccggccagg cttcgcatct tggtgctcag accggcgaac aggaagatcc cggcgaagat
      841 gatcgtgctg atcacgtatt tgtcgtcgtt gtcgtcggcg gcgtgggcca gcgccgactt
      901 gtcctgagct tcctggagga ggcgttccgc ctctatttct tgggcaactt gatactcctc
      961 catggcgaac ggggtcggtg gtgcgtccgg gttggatagg ggccgggtgg ccagccaggc
     1021 gtccatcgct gtcttgaact cggggcgaaa acgggcatag agaaatccgg agagtgtcgc
     1081 gggatcgggg tggtagccgt cgctcacatc tatctcgccc gagtcgttct cctgttttgc
     1141 ggcatccagc caggcgagaa aggtggcaac gtcaatcgat gacaactggc cagccctggt
     1201 gaaacttcga gtggactcgg tccgagcggc gccagcctcg ttgagcgaga aggacatggt
     1261 gtccccccac tggtcgcttt ggaacgccga ccaggcagtg gccagtgcgg cgaaggccat
     1321 cacgatcgtc gccacgatgt cgatccaatc gagcggctgg tgtttcggct cggtcgttgc
     1381 aacctcggct tcggctgtca ccgcactcaa ccctctctac tttccaaggc ctcgatcagt
     1441 ttgggcagca ctttgtggac atcaccgacc acaccgagat cagcgattga gaagatgggg
     1501 gcgtcggggt ccttgttgat ggcgatgatc gtcttcgaat ccttcatccc caccagatgc
     1561 tgcatggcac ctgagatccc acatgcgatg tagacgtcgg gcttgaccgt cttcccggtc
     1621 tgaccgacct gtttcgcata gggcacccat ccggcgtcga cgattgccct ggtggccccg
     1681 gtggccgcac cgagcttctt gcccagcctc tccaccaact cgtaggcctc cggtgagccg
     1741 agaccacgac cacctgacac cacgaccgct gcctctccaa gttggggccc ttcgcgctcc
     1801 tcgacgtgcg attcggtcac ggtggcttct gaccgtgcgg gatcgggcag accgatctcc
     1861 tccaccgccg gcatcggccc gccaacagcc tcagcgacga acgacttggg acggatcagg
     1921 accaagagcg ggccctgggt catcgatgcc gttgccacct gggcgccgcc caggatctcg
     1981 tggtcggtct cgaccccgcc atcgactagc cgcaccgcga tcgcattcga gaggacgccg
     2041 actccgaggc gagcagcgag gcgcccagcc acgtcgcggt cggtgtaggc ctggccgaag
     2101 atgatcagag ccggagcctg atcggcgatc ctggcggcca gagccgtggt caccggccca
     2161 gccggaagcc ggctgcctgc gtcgaggtgg agcaccgaag tagcgccatg ctctccgagg
     2221 gtctcaaagg ccccgtccga tccggtgccg aggtagatag cagtgacctc accgagttga
     2281 cggccggccg ccagcaactc gaggccgatg gttgtcgggc tgcccccctc ctcctcgacg
     2341 aagacccaga tggcggccat ctcagatcac cttccgttgt tcgagaaggg cgacgatggc
     2401 cagatgcgac tcaccgtcgt cctcgatctt ttgaccggcc gtacgctcgg ccgcgtcggt
     2461 gatcgaggca acggtctggt cgggtcccct ctcaacccca aggtcggcaa cgctgaggct
     2521 ctcgaccgtc ttcttcttgg cgtccatgat tcccttgaag gtcgggtagc gcggttcgac
     2581 cacaccggcg gtgactgcga cgaccgcggg caggctggcc tcgaccacgt cgtatccaga
     2641 ggcggtctgg cggtggatgg ttactttccc gtcggcggcc tcgactttgg tggcatatgt
     2701 cagcgcaggc acgtcgagta gctcggagag catctgaggc aggactcccg agtagccatc
     2761 ggttgactcg gtcccggtga tcaccagatc gaagccgtcc ctctggatgg cggccgccag
     2821 gacccgggcc gtggtcaggg cgttggagcc ccgaagcgtg tcgtcctcga tgagaatcgc
     2881 ctggtcggcg cccatggcca gagcctggcg gattccttgc ccgttccccg ccgggcccat
     2941 gcacaccagg gtcaccgtcc cttcactggc ctgagccaat tggagtccca tctcgacccc
     3001 gtagcgatcg gtgtcgtcaa ggatctggtc gtcgggccgg ag
//
LOCUS       DBNHXU010000338         2671 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3894205, whole genome shotgun sequence.
ACCESSION   DBNHXU010000338 DBNHXU010000000
VERSION     DBNHXU010000338.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2671)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2671
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3894205"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..594
                     /locus_tag="AC161X_08345"
     CDS             <1..594
                     /locus_tag="AC161X_08345"
                     /inference="COORDINATES: protein motif:HMM:NF019310.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4989600.1"
                     /translation="LAAPFTSGVGIWAFYAFQPYLLQLFGDPNATFLSGIAAAVFALA
                     QIIGGSSVRWVRRIFATRTSVLVGQVMVASLALVGAGLAEWLEIPIGFWVAIVLLTIM
                     SLVGALASPLQQALLNDCIPSEQRATVLSFVSLTGSAGGVVTQPALGKVADVYSLGVG
                     YIVAGGIYVLSLPFVIAVRRMHLTADRVVRAEPTIVS"
     gene            614..832
                     /locus_tag="AC161X_08350"
     CDS             614..832
                     /locus_tag="AC161X_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989601.1"
                     /translation="MEDHDTSSEPSGQAISRGAMIGVPVGIVGMTIAVWLITGLDFWE
                     CFATAIMPGVLLGAFSGGFVGMTMSMGE"
     gene            complement(999..2435)
                     /locus_tag="AC161X_08355"
     CDS             complement(999..2435)
                     /locus_tag="AC161X_08355"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020548946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004030 - aldehyde dehydrogenase [NAD(P)+]
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR4989602.1"
                     /translation="MATDLKLFIAGEWTEGTGNDHYEVRSPSTGEHIYNVPKASPADI
                     DRAVKAAREATDEMRQWTAFERADLCLRIYDLWKTRVNEVARILSTEQGKPYEAEALD
                     DIAESGDYFQIAAEDVKRLKGELIPTTSRHRRMFTMRKPVGVWVAITPWNFPVMIPME
                     YVGPGLATGNAVIVKPPEFTSWALLEMAKVFEEAGAPKGAVSVIPGAGDIGTALVTHD
                     GVDAVGFTGSSATGKKIVAAMGLKRSIMEMSGNGPTIVTADADISKAAQAAVYGAYYN
                     AGQVCVATERVIVVDEVHDEFVEEALKAAQVVSLGDPFDESTNMGPLNNEPTAAKMDR
                     HIADAVNHGAELLMGGGRAAGFPTDLYYQFTMLDRVSEESEVSQEESFGPVLPILSAK
                     DDEDAVGVANRTHLGLQAAVFTNDLSKAFWYADRIRSGTVIVNDSTDFWETFQPFGGA
                     AGTDTGWGRQRIDEFTDLQTVVFVLKEE"
BASE COUNT          436 a          841 c          850 g          544 t
ORIGIN      
        1 ctggcggcgc ctttcaccag cggggtgggg atctgggcct tctatgcgtt ccagccctac
       61 ctcctccagt tgttcggcga tcccaacgcc accttcctct ccggtattgc cgccgcggtg
      121 ttcgccctgg cccagatcat cggcgggtct tcggtgagat gggtgcggag gatcttcgcc
      181 accaggacga gcgtgcttgt cggccaggtg atggtcgcct cactggcact ggtcggtgcc
      241 ggtctcgccg aatggctgga gatcccgatc gggttctggg tagccatcgt cctcctcacc
      301 atcatgtcgt tggtaggtgc tctggccagc ccgttgcagc aagctctgct caatgactgc
      361 atcccatcgg agcaacgggc caccgtgctc tcgttcgtct cgctgacggg gagcgccggt
      421 ggggtggtca cccagccggc actggggaag gtcgccgacg tgtactcgct cggagtcggc
      481 tacatcgtcg ccggagggat ctacgtcctc agccttccgt tcgtgatcgc ggtccggagg
      541 atgcacctga ccgcggaccg ggtggtcagg gccgagccga caatcgtctc ttgagttctg
      601 gcaagattcg gtgatggaag accacgacac ctcgtcggag ccatccggtc aagcgatttc
      661 cagaggggcc atgatcgggg tgccagtggg gatcgtcggt atgaccatcg ccgtgtggct
      721 gatcaccggt ctcgacttct gggaatgctt cgctaccgcg atcatgcccg gtgtcctgct
      781 cggcgccttc agtgggggtt tcgtcgggat gacgatgagc atgggggagt aggggcccca
      841 agctccgacg cgtggcgggt agcccccctc cccggcttcg ccggtactcc ccccaccgct
      901 cactccgttc gcggggggga gaaaccgttt cctcccccag gagcggagcg acgttggggg
      961 aggtggcccg aagggccgga gggggctgct cctgcgcgtc attcctcttt caacacgaac
     1021 accaccgttt gcagatcggt gaactcgtcg atccgctgtc ggccccaccc ggtgtcggtc
     1081 ccggcggcgc ctccgaacgg ctggaaggtc tcccagaagt cggttgagtc gttgacgatc
     1141 acggtgcccg agcggatccg atcggcgtac caaaacgcct tgctcagatc gttggtgaac
     1201 actgctgcct gcagtccgag atgggtccga ttggcgacgc caacggcatc ctcatcatct
     1261 ttggcactga gaatcgggag taccggcccg aaggactcct cctgtgagac ctcggattcc
     1321 tcgctgaccc ggtccagcat cgtgaactgg tagtagagat cggtggggaa gccggcggcc
     1381 cgaccgcctc ccatcagcag ttcggctcca tgatttaccg cgtcggcgat gtgacggtcc
     1441 atcttggccg cggtgggctc gttgttcagt gggcccatgt tcgtcgactc gtcgaagggg
     1501 tctccgagac tcaccacttg tgccgctttg agcgcttcct ccacgaactc gtcgtgaacc
     1561 tcgtccacca cgatgacccg ctccgtggcc acgcagacct gacccgcgtt gtaataggcg
     1621 ccgtaaaccg cggcctgagc agccttcgag atgtcggcgt cggcggtaac gatggtcggc
     1681 ccgttgcctg acatttccat gatcgagcgc ttcagcccca tggcggcgac gatcttcttg
     1741 ccggtggcgg acgatccggt gaacccaacc gcgtcgacac catcgtgggt gaccaaagcg
     1801 gttccgatgt cccccgcacc gggaatcacc gagaccgcac ccttcggggc gccggcctcc
     1861 tcgaacacct ttgccatctc gagtagcgcc caggaggtga actcgggggg tttgacgatc
     1921 accgcgttgc ccgtggccag ccccggtccc acgtactcca tagggatcat caccgggaag
     1981 ttccatgggg tgattgccac ccagaccccg accggtttgc gcatcgtgaa catgcggcga
     2041 tggcggctgg tggttgggat cagctcccct ttgagccgct tcacgtcttc ggctgcgatc
     2101 tggaagtagt cgccggactc ggcgatgtcg tcgagtgcct ccgcttcgta aggcttgccc
     2161 tgctccgtgg agaggatccg ggccacctcg ttcacacggg ttttccagag gtcgtagatt
     2221 cgaagacaga gatcggcccg ctcgaaagcg gtccactgcc gcatctcgtc cgtcgcttcg
     2281 cgggccgcct tgaccgcacg gtcgatatca gccggggacg ccttgggaac gttgtaaatg
     2341 tgctcgccgg tggacgggga cctcacctcg tagtggtcat tacctgtgcc ttcggtccac
     2401 tcgccggcga tgaagagctt gagatcggtc gccatgcacc aaggctagtt tcccgtcatc
     2461 ccccgggtta aacgactgtt cagtacgatg ccgggagagg agtgagagat atgagtctcc
     2521 agggagacaa gaccagacag tggtaccagc gagcttcgaa agtccttgtc aacggcgtct
     2581 ctagccagtt tcgatattgg ggcgacgaag acaccatggt catcgaccat ggggaggccg
     2641 gctatgtgtt cgacatggac ggcaagcgct a
//
LOCUS       DBNHXU010000339         1553 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3496131, whole genome shotgun sequence.
ACCESSION   DBNHXU010000339 DBNHXU010000000
VERSION     DBNHXU010000339.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1553)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1553)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1553
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3496131"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..929)
                     /locus_tag="AC161X_08360"
     CDS             complement(<1..929)
                     /locus_tag="AC161X_08360"
                     /inference="COORDINATES: protein motif:HMM:NF012998.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004664 - prephenate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0009094 - L-phenylalanine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase domain-containing
                     protein"
                     /protein_id="HIR4989603.1"
                     /translation="MSTENEQHEDDITQIRDDIDRVDAEILKLLAERKAFSLRMAREK
                     GLHARPSRDMSREEDLISDRINSGMSHDLDSGLVNRVWREIINDSVRVQQEFLGRTES
                     TPDSIVVAIQGIDGSYSHLASQQFFDAKGITPSYVRSSTFADAIRAVQEGNADVAVLP
                     VENTTSGAISEVYDVLLDSQLHFVGDVRFRVRHCLLGVAGASIEGLRRIYCHPQAVTQ
                     CSEFLAGLDHVELVYFADTALSGRRIGEMNDPTVAAIASEEAARLYGLDILKTGIANQ
                     EENYTRFVVVAGQPAAVPEGVQSKTSIVMAVGN"
     gene            1145..1354
                     /locus_tag="AC161X_08365"
     CDS             1145..1354
                     /locus_tag="AC161X_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020642128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989604.1"
                     /translation="MNRTRLSFGIVLVLVGAVWALQGLNAGFVPESAMTGETTWVVLG
                     SLAVLVGIGLIWWSQTPSKPEDKNQ"
BASE COUNT          299 a          445 c          499 g          310 t
ORIGIN      
        1 tggttgccga ccgccatgac gatcgaagtc ttcgactgca ccccctcggg caccgcagct
       61 ggttgcccgg cgacgaccac gaagcgggtg tagttctcct cctgattggc gatgccggtc
      121 ttgaggatgt cgagcccgta gagacgggca gcctcctcac tggctatggc ggccactgtt
      181 ggatcgttca tctctccaat tcgcctgccg gagagggccg tatccgcgaa gtagacgagc
      241 tccacatggt cgagcccggc caggaactcc gagcactggg tgacggcttg gggatggcag
      301 tagatacgcc gcaatccttc gatcgaagcg cccgccaccc cgagcaggca atggcgaact
      361 cggaaccgga catctcccac gaagtggagc tggctgtcca agaggacgtc gtagacctcc
      421 gagatcgcgc ccgaggtggt gttctccacc gggaggacgg cgacatctgc atttccctct
      481 tgcacggcgc gaatcgcatc ggcgaatgtc gatgagcgga cataactcgg tgtgattcct
      541 ttggcatcga agaactgctg tgaggcgaga tggctgtagg agccgtcgat cccctggatg
      601 gcgacaacga tcgaatccgg ggtcgactcg gtgcgaccca agaactcctg ttggacgcgg
      661 acggagtcgt tgatgatctc tcgccacacc cggttgacca gtccggagtc gaggtcatgg
      721 ctcattccgg aattgatccg gtccgagatc aaatcctcct cacgggacat gtcgcgggag
      781 gggcgggcat ggagcccttt ctcccgggcc atgcgaaggc tgaacgcctt gcgctcggca
      841 aggagtttga ggatttcggc gtccacccgg tcgatgtcgt cccggatctg ggttatgtcg
      901 tcttcgtgct gttcgttttc ggtgctcatc tcattcactg ctttcaggac gcgttgcgtc
      961 agaggggtag gtcaggagta acgcgggggt gggtaaatcg gcgctaggcg ccgaaataga
     1021 agaagtaggt gaacccgaag aagcgctgag cagcgccgaa gaaattcagg gtccgagtcg
     1081 acttcatgtg gtggtgatta tccacagacg gggcacgcca tgtcaagagc tagggtcagc
     1141 ctcgatgaat cggacccgtc tttcctttgg aatcgtcctg gttctggtcg gggcggtgtg
     1201 ggcgctgcag ggtctcaacg ccggcttcgt accggaaagt gccatgaccg gcgagacaac
     1261 atgggtggtt ctcggctcac tcgcggtcct cgtcggtatc ggcctcatct ggtggagtca
     1321 aacgccaagt aagccagagg acaagaacca gtaggagagg gtgcgcggga taagctgagc
     1381 ggtcgaggcg gtcatatgag ccaacgaccc gagcgccctg gggtagccct tccaccggaa
     1441 gagctacagc gcttcatgta tgcctgttcc cacgagcggc gctcccccac cacacagcgt
     1501 gtgatcgacg gttcctccta cggctggatc caccttttca ttcgcagcaa cgg
//
LOCUS       DBNHXU010000340         4928 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5085979, whole genome shotgun sequence.
ACCESSION   DBNHXU010000340 DBNHXU010000000
VERSION     DBNHXU010000340.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4928)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4928)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4928
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5085979"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..769
                     /locus_tag="AC161X_08370"
     CDS             182..769
                     /locus_tag="AC161X_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015749330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR/SmtB family transcription factor"
                     /protein_id="HIR4989605.1"
                     /translation="MTDLSSPDYELDETLDFQNAEQFRALFEETRLRIVELLLERAAT
                     VTELAAVLDKPKGTVGHHVGVLEHAGLIQVVRTKKVRAIEAKYYGRTARTYLLTPQVD
                     VDLHVAPDYFLTSAAAEFAKASHGEEEMIHNTMISTLRYARIPADRAREWGLRLGELA
                     QEFVSEERGGDTTFGLLVALYPTDRPHLPDLMDEK"
     gene            766..2115
                     /locus_tag="AC161X_08375"
     CDS             766..2115
                     /locus_tag="AC161X_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019851927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4989606.1"
                     /translation="MTTPLGRSYWKLWTASVISNFGDGVSFIAYPWLASAVTRDPVQI
                     SLVAVATRLPWLIFTLPAGVITDRVDRRRLVAWMDVVRFLLTLGVAFIVLGVQDQLAS
                     PDAIAAGTAALPDSATLLLVMIYAASLLLGFAEVLRDNSAQTLMPAIVVDENLEKANG
                     RLWGAEQVMNSFVGPPVGGFLLAIGFALPFFVDAGTFGVAAALVFMIGGEFRAKKSPS
                     QTQVKPSFKAEMKEGVRWLWHHPLFRPMAISLGVLNAMSSLAFATFVLFAQEILELDA
                     AGFGILMTAGAAGAVIGSFIAAWVSKKIGQGPSLFVTLAGGAITFLVIGLTSSGLVLW
                     TMVALMTLTSVLWNVITVSLRQSLIPDHLLGRVNSVYRFFGWGMMPIGSVLGGLIVVL
                     AEPLVGREWALRWPFIIGALVHVALFFYAVPNLNSRRIEEARAMAKTQESGVESVGD"
     gene            complement(2082..2648)
                     /locus_tag="AC161X_08380"
     CDS             complement(2082..2648)
                     /locus_tag="AC161X_08380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989607.1"
                     /translation="MSRILAVALLASVNLGGGFGNASAEVLSINDESMEIEVRVEVQS
                     SADAVVVHFALSDEDPVTLPLVSRGDGVFGIVTELKPANYQVLFETLGEPSVQSQAVS
                     LTDLGADIGDVGQEITSTTEEGVSDATQGWGWLAVAFGAASLAALAFWVLGGRDDREA
                     VVDPDPDTSADDEPAAEDQSPTDSTPDS"
     gene            complement(2697..3485)
                     /locus_tag="AC161X_08385"
     CDS             complement(2697..3485)
                     /locus_tag="AC161X_08385"
                     /EC_number="3.1.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF015000.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008081 - phosphoric diester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase family
                     protein"
                     /protein_id="HIR4989608.1"
                     /translation="MIPSHWAPPIAIAHRGSRELWPENTMEAFSQAVALGFRFLETDL
                     HVTADGVLVCIHDDTVDRTTDGTGSVSNFGFEELASLDAGFRHNAAGGYPFRGAGVSV
                     PSLEEAVTTLPEVSFVVDLKGDGQVDLLAALIERLALHDRLIVGSFSDQRLDEFRSAT
                     GGRVATSSGPMLSRMWLLTSRVGRGAGGQASALQLPTQVRGIKVVDKKLVDTAHNNGL
                     QVHVWTVNDPAEMESYLDMGVDGIISDRPDLLRDLLVSRGQWPY"
     gene            complement(3550..3843)
                     /locus_tag="AC161X_08390"
     CDS             complement(3550..3843)
                     /locus_tag="AC161X_08390"
                     /inference="COORDINATES: protein motif:HMM:NF012442.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030527 - structural constituent of
                     chromatin [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HIR4989609.1"
                     /translation="MNKSEMADKLADKADVSKAKAAEIIDIIFSTKDGEGIIAIELDA
                     GRKVNITGFGNFSLKSRSARMGRNPATGQTITIPAKNYAHFAASKGLKDRVAI"
     gene            complement(4006..4413)
                     /locus_tag="AC161X_08395"
     CDS             complement(4006..4413)
                     /locus_tag="AC161X_08395"
                     /inference="COORDINATES: protein motif:HMM:NF023010.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3237 family protein"
                     /protein_id="HIR4989610.1"
                     /translation="MPELEKFCSIDASLGMKILGKAPQGMRIDFPFEGVAVGPHWEGE
                     RPVVGIDHATVREDGNMDLVIFGVIGEKRESVAYTARGVSIANPDQSADPKELVTFQT
                     GNEDLSWLNSEVAVALGHGEAGRLTLELYLVKS"
     gene            complement(4444..>4928)
                     /locus_tag="AC161X_08400"
     CDS             complement(4444..>4928)
                     /locus_tag="AC161X_08400"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012383.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase"
                     /protein_id="HIR4989611.1"
                     /translation="VRKVGVPYQVELAMGAVGERGVVVRNREVMRAAGITEAAFQAVV
                     AAETAELEARVGIYREVAAPIEHAGRTVIVTDDGLATGSTALAAIDIARAGRAREVWV
                     AVPVAPADSIRKVAKVADQVVVLQRPQRFMAVGAWYRDFSQTTNDEVRDLLRRSREGR
                     "
BASE COUNT          901 a         1593 c         1464 g          970 t
ORIGIN      
        1 gtgcggacgt ccagatccaa cgaaccaagg aagtcaccgg tgaacactcc tcgatacgcg
       61 acctgccagg tggcaatgtg gacgccggcg attccaccgg cgtcggccgg caccgcaatt
      121 ctcaccatca agccaaccta aactagttga caattggtcg ggaattgtcg accatacggg
      181 aatgaccgac ctttcatcgc cggactacga gctggacgag accctcgact tccagaacgc
      241 cgagcagttt cgtgcacttt tcgaagaaac caggctccgg atcgtcgaat tgctcctgga
      301 gcgagctgcg actgtgaccg agctcgccgc cgtgctggac aagccgaagg ggactgttgg
      361 ccaccatgtg ggcgtcctgg agcatgcggg cctgatccag gtggttagga ccaagaaggt
      421 cagggctatc gaagcgaagt actacgggcg aactgcccgg acgtatctcc tcacaccgca
      481 agtcgatgtc gatctccacg ttgcccccga ctacttcctg acgtcggcag cagccgagtt
      541 cgcaaaggcc tcccacggtg aagaggaaat gattcacaac accatgatca gcaccctgcg
      601 ctatgcccgc atccccgctg atcgcgcccg agagtgggga ctccgactcg gcgagctggc
      661 tcaggaattc gtgagcgaag agagaggtgg agacactacg ttcggcctgc tcgtcgccct
      721 ctaccccacc gaccgtcccc atctccccga tctcatggac gagaaatgac cacgcccctc
      781 ggtcgcagtt actggaagtt gtggaccgcc tctgtcatat ccaatttcgg agacggagtg
      841 tccttcatcg cctatccctg gctggcgtcg gcggtaaccc gcgatccggt gcagatctcc
      901 ctggtagcgg tcgccactcg cctcccctgg ctcatcttca cccttcccgc tggcgtgatc
      961 accgatcggg tggaccggcg gcgactggtg gcatggatgg atgtggtccg gttcctgctg
     1021 acgctcggtg tggctttcat tgtgctcggt gtccaggacc agctggcatc accagacgcg
     1081 atcgcggctg ggaccgcggc actccccgat agcgcgactc tgctcttggt gatgatctat
     1141 gccgcctcgc tccttctcgg cttcgccgaa gtgctccgcg acaactcggc acagacactg
     1201 atgccggcaa tcgtcgtcga cgagaacctg gagaaggcca acggccggct gtggggcgcc
     1261 gagcaggtca tgaactcctt cgtgggacca cccgttggtg gcttcctcct cgctatcggt
     1321 tttgcgcttc cgttcttcgt cgatgccggg acgttcgggg ttgccgcggc cctggtcttc
     1381 atgatcggtg gcgagttccg ggccaagaag agcccttccc agacccaggt caagccttcg
     1441 ttcaaggcgg aaatgaagga aggcgtgcgc tggctgtggc atcacccgct cttccgccct
     1501 atggccattt ccctaggggt gctgaacgcg atgtcgagcc tggccttcgc caccttcgtg
     1561 ttgtttgccc aggagattct cgagctcgat gccgccgggt tcggcatcct gatgacggca
     1621 ggagccgcag gcgctgtgat cgggtccttc atcgccgcct gggtttccaa gaagatcggt
     1681 caggggcctt ccctgtttgt caccctcgcc ggcggcgcca tcaccttcct ggtcataggc
     1741 ctcacctcat caggtctcgt gctgtggacg atggtggcgt tgatgactct gacttcggtg
     1801 ttgtggaatg tgatcacggt gtcattgcgc cagtcgctga tccctgacca cctgctgggg
     1861 agggtcaact cggtgtatcg cttcttcgga tgggggatga tgcccatcgg ctcggtcctc
     1921 ggcggtttga tcgtggtgct cgccgagcct ttggtcggac gggaatgggc tctgcggtgg
     1981 cccttcatca tcggggcgct ggtccacgtg gccctcttct tctacgcggt gcccaacctc
     2041 aacagtcgtc gcatcgaaga ggcccgcgcc atggcgaaga ctcaggagtc aggcgtggag
     2101 tcggtcgggg actgatcctc agctgccggc tcgtcatctg ctgaagtgtc cgggtcaggg
     2161 tcaaccacgg cttcgcggtc atccctgcca ccgaggaccc aaaaagccag ggctgccaga
     2221 gacgccgctc cgaaggccac ggcgagccag ccccacccct gcgtggcgtc gctgaccccc
     2281 tcctcggtgg tgctggtgat ctcttgaccg acgtcaccaa tgtcggcgcc tagatcggtc
     2341 agggacacgg cttgggactg gaccgagggc tcgccgagag tctcgaagag aacctgatag
     2401 ttcgctggct tcagctcggt gacgatcccg aaaactccgt ctcccctcga aaccagggga
     2461 agggtcaccg gatcctcgtc tgaaagggca aagtggacca ccacggcatc ggccgagctc
     2521 tggacttcta cccggacctc gatctccatc gactcatcgt tgatcgagag gacctcggca
     2581 ctggcgttcc cgaagccccc acccaggttc accgatgcga gaagggcaac agcaagaatt
     2641 cgggacatcg ccctagatgg tacgacctcg accctgcttc gattcgaaaa ccaaggtcag
     2701 tagggccact ggcctctcga aactaggaga tcacggagca gatcaggacg gtccgagatg
     2761 atcccatcga ctcccatgtc gagataggac tccatctcgg ccgggtcatt gacagtccac
     2821 acgtggacct gaagtccgtt gttgtgcgcg gtgtcgacaa gcttcttgtc gaccaccttg
     2881 atcccgcgga cctgtgtcgg caattgcagt gcgctcgcct ggccaccggc cccccggccc
     2941 accctggaag tgagcagcca cattcggctc agcatcggcc cggacgaagt tgcaaccctg
     3001 cccccggtgg cgcttcggaa ctcgtccagg cgctggtccg agaaggagcc gacgatcaat
     3061 cggtcgtgaa gtgcgagcct ctcgatcagt gcggccaaga gatcgacctg accatcgcct
     3121 ttcaagtcga ccacaaacga cacttcgggc aacgtggtga ctgcctcctc gagactgggc
     3181 actgacactc cagccccccg aaatgggtac ccgcctgcgg cgttatggcg aaatccggcg
     3241 tccaggctcg ccaactcctc gaaaccgaag ttgctcacgg atcctgtgcc gtcggttgtt
     3301 cggtcgacgg tgtcgtcgtg aatgcagacc agcacaccgt ctgcggtcac atggagatcg
     3361 gtctccagga agcgaaaacc aagcgcaact gcctgggaaa acgcctccat cgtgttctcc
     3421 ggccagagct cccggcttcc ccggtgggcg atggcgatgg gtggggccca atgcgatggg
     3481 atcatatgtc ggccaaaata atggaccccg ccagaggcgg ggcccaccaa attcccggtt
     3541 gaatggctct cagatggcca cgcggtcctt gagacccttg gacgcagcga agtgcgcata
     3601 attcttcgcc ggaatcgtga tggtttgccc ggttgcgggg ttgcggccca tgcgtgccga
     3661 acgggacttg aggctgaagt tgccgaatcc ggtgatattc accttgcgcc cggcatcgag
     3721 ctcgatggcg ataatgcctt cgccatcctt ggttgagaag atgatgtcga tgatctcggc
     3781 ggccttcgcc ttggagacat ctgccttgtc ggcgagtttg tccgccatct cggatttgtt
     3841 cacggcccct ccttgtgggt cgacgattgg gcctgtcgct tagcgacgat gaggccacgt
     3901 taggcgaaaa cccggaaagt tccgatcatt actggttttt agtctcaagt cgcaggtcac
     3961 aagccaaaga atcttgcctg agacttgaga cttgagacag gggactcaag acttgaccag
     4021 gtacagctcc agggtgagcc ggcccgcctc gccgtgaccg agagcgaccg ccacctccga
     4081 gttgagccaa gagagatcct cattgccggt ctgaaaggtg accagctcct tggggtcggc
     4141 cgactgatca gggtttgcga tggagacccc tcgtgcggtg taggccactg actcacgctt
     4201 ctccccgatc accccaaaga tgaccagatc catgttcccg tcctcgcgga ccgtcgcgtg
     4261 gtcgatcccc accaccgggc gctcgccctc ccaatggggc cccacagcca cgccttcgaa
     4321 cggaaagtcg atcctcatcc cctgaggcgc tttgcccagg atcttcatcc caaggctggc
     4381 gtcgatgctg cagaacttct ccaattcggg caatggtcct ccacgtctgg ttggcgatcg
     4441 acgctagcga ccttctcggg agcgacggag aaggtcgcga acctcgtcgt tggtggtctg
     4501 agagaaatcg cggtaccacg ccccgaccgc catgaagcgc tgtggcctct ggagaaccac
     4561 cacctgatcg gcaactttcg cgaccttgcg aatcgagtcg gcgggcgcga ccggcaccgc
     4621 cacccacacc tctctggccc gtccggctcg ggcgatgtca atggccgcca gggccgtcga
     4681 accggtagcg aggccatcat cagtgacgat gacggtgcgc ccggcgtgtt cgattggcgc
     4741 cgccacctcg cgatagatgc cgacccgggc ctcgagctcg gctgtctcgg cggctaccac
     4801 cgcctggaag gccgcctcgg tgattccggc ggccctcatc acctcacgat ttcgcaccac
     4861 cacgccccgc tcacccaccg cccccatcgc caactcgacc tgatagggaa ctccgacctt
     4921 tcgcacca
//
LOCUS       DBNHXU010000341         6294 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_720116, whole genome shotgun sequence.
ACCESSION   DBNHXU010000341 DBNHXU010000000
VERSION     DBNHXU010000341.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6294)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6294)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6294
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_720116"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1087)
                     /locus_tag="AC161X_08405"
     CDS             complement(<1..1087)
                     /locus_tag="AC161X_08405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989612.1"
                     /translation="MTLQRRAGQGFPWPTFLILTGISLVILIVLNPGWILSATTPTGG
                     DMGAHVFGPAYLRDNLLPHGRILGWSNDWFAGFPAFYFYFPLPSLVIVLLDVVLPYGV
                     AFKVVTVIGLLGIPPAAYFLGRSMLLGRQVSIVAAGSGAVFAFLESYSIYGGNVASTL
                     AGEFAYSWSFALSLVYLALLIKAVREDKKYVKWAALAFAATLLSHVLTTIVIVFASLF
                     VLPWRRGFWRTMAIGFWGFAIAAFWAVPLAARIGLTSDMAWSPLTRWEEVFPVELWLL
                     IPLAIPGAIWASRHTSRAGPLMAATLLPLIYYPLPTVLPELFPSLFGGERWKLWNGRL
                     LPYWYFGVAFFAAVGVGAAVLWASRRLP"
     gene            complement(1084..1260)
                     /locus_tag="AC161X_08410"
     CDS             complement(1084..1260)
                     /locus_tag="AC161X_08410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989613.1"
                     /translation="MDCPQCSQPDVIEIEHLLPDGTEVRFFACHNCEEKWWNKDGVDI
                     DVAQVLEIVRQHRT"
     gene            complement(1310..1588)
                     /locus_tag="AC161X_08415"
     CDS             complement(1310..1588)
                     /locus_tag="AC161X_08415"
                     /inference="COORDINATES: protein motif:HMM:NF042840.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6788 family protein"
                     /protein_id="HIR4989614.1"
                     /translation="MALDRDLLDAVKDLDEHDLRRLLILTRAQLQKAGALAAEDHPRV
                     RLRKQSVRCGKAGCNSCPHGPYWYAYWTEGGRRRSRYVGRLLEEGPLS"
     gene            complement(1588..1809)
                     /locus_tag="AC161X_08420"
     CDS             complement(1588..1809)
                     /locus_tag="AC161X_08420"
                     /inference="COORDINATES: protein motif:HMM:NF023430.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3499 family protein"
                     /protein_id="HIR4989615.1"
                     /translation="MVLSCVRCGSPSAALMTFSYPEAAVWLDDLVVDSAPFGYSLCAD
                     HADRMIPPQGWTLTDRRTVTRLFAPLSVA"
     gene            complement(1842..2207)
                     /locus_tag="AC161X_08425"
     CDS             complement(1842..2207)
                     /locus_tag="AC161X_08425"
                     /EC_number="1.8.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HIR4989616.1"
                     /translation="MSEVEVRLIAVIGAVGVGMALSLILRRRPTQRSPSELKQNRLGP
                     GVYLFTSEACPDCEAVRAVLLERFGPVGFSELSWESDPEIFRELGVDAVPATLVVTAT
                     EGSTLYPGSTEPRQGGVSP"
     gene            complement(2200..3477)
                     /locus_tag="AC161X_08430"
     CDS             complement(2200..3477)
                     /locus_tag="AC161X_08430"
                     /inference="COORDINATES: protein motif:HMM:NF040160.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5719 family protein"
                     /protein_id="HIR4989617.1"
                     /translation="MIRTLMVVVLAAGAVALPAPGSPQPAPAAPVDTPAMAVCAVEEG
                     SGRATNINVVSSVDGSGALTAFAAGATVGSVEFETNAATGSSIAIADIAAVGVGGALL
                     ELPAVASAAASVVVGADSLAMDTCPVAAGGDLILGGGSTLSDQRFEVQLMNPYAGEAI
                     VNLVVLSDAGLESSEALDTVIVPARSTVLVDLAEILPGREWMTVAIEVESGGVLAVGR
                     LGIGTDNAIWDAVAPAQDWFIPVPHGLESRQVVIAARDSDVEYQIDFYGPDGLVEALE
                     SGQIPARGQAVVDVGAVSPVASAVRVVTTAPVGAFLRISSATGVALTSGTTSVASVLL
                     LPGVGSVAGGSGWLALLNPGLEDVSAVMTALGSGSGSETISVPAGEVAEIEISPDPVL
                     GYTIGGDGPMVATWSTTLGGSIGLGIAAPIPDE"
     gene            complement(3483..>6294)
                     /locus_tag="AC161X_08435"
     CDS             complement(3483..>6294)
                     /locus_tag="AC161X_08435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989618.1"
                     /translation="DSHRQSSILLSVATRFTMTHRMTDASTPLMAEESTTDPSASAPK
                     IKVVVTLVEGADLEAALATVDRQVYDSVEEVIVVGDSGLEVPDGIGRSVTLEAAIAST
                     SSDVEYLWILHSDARPRPDALAALVSEVERNDASLGGSKLLVAGTRDELESIGSATDV
                     FGEPYSGLDEGEIDLQQYDVVREVAFVSSVSMLVRRDLAQGLQGLDDLLEPVAAGLDF
                     SQRVRLAGGRVITVPSSEVFHQGRCRQAGRGWREQAGRMRAMLKAYRPITLAWVIPYQ
                     LFVGIVDSLFNLVLLRWRPAAKHVASWGWNLVHLPSTIAARRKFKPVRAFGDEELFRF
                     QARGSVRLRDLGAELTERLLFMFDDDKALSRGTKRIWSSPGIWGAVIAAALAVVAMRS
                     IIFTGVPNAGFSFDFEPPSVALSRFFGGWNDSGLGSPGAVHPSTGMTGLASVVWFGAE
                     GAARLLITVGAAFMAVLGMGRLSGRLGLRGPGRYLSGLVLLAGPGAAVLVGAGSWLGL
                     IAASLLPWAVRSVFVHPNDSEGKPLSRFAWVIAWSLLLGAMSPVLVVVPLITVLLARA
                     LGDDRSRMWLAVAGLVGILVALPFLFGDPGWLIDANRRLGLGVDSSWPFLLIIGVVPL
                     LFLDNGIGRLALTGGILSLASLVLLDVPLGGPGYEEALLIISSFGAAIVVASALDRLS
                     LEPKRVLPAAAAIAVLILSVGSMADGRLGLPSGDINGKYGFAATLAGDEGPGRILVLS
                     EDRAMIPGETRSGPGLWYRLIDGSRMTQDEAWLPEPLPGDEKLSAAIDQMASGADLRP
                     GASLAEFSIDWVVIEGPETYLDQVLAVQLDLVPTPLDPTARIYGNPNSMAMASGSATT
                     WSRVGTGFGGDITSARIRLSINADPRWSPDPQAADWAVSVAGDQGSGRYRPDPTGLAL
                     SIAAIALLVAAFAAVVVGRLRR"
BASE COUNT         1375 a         2050 c         1949 g          920 t
ORIGIN      
        1 atggaagccg tcgagaagcc caaaggacgg ccgccccaac tccgactgcg gcgaagaagg
       61 cgacaccgaa gtaccagtag gggagcagcc gcccattcca caatttccaa cgctcgccgc
      121 cgaacaggct ggggaacaac tcggggagga cggtcggaag ggggtaatag atcaaaggca
      181 gcaaggtcgc agccatcaat ggcccggccc gcgaggtgtg acggcttgcc caaatcgcac
      241 cgggaatcgc caacgggatc aggagccaca gttcgaccgg gaagacctcc tcccaacggg
      301 tgagcggaga ccacgccatg tccgaggtca acccgatccg ggcggccagg ggcaccgccc
      361 agaacgccgc tatcgcgaac ccccagaagc cgatcgccat ggtccgccag aaaccccggc
      421 gccatgggag gacgaacaac gaggcgaaca cgatgacgat cgtcgtgagg acgtgggaga
      481 gcaacgtcgc ggcaaacgcg agagccgccc atttcacgta cttcttgtct tccctcaccg
      541 ccttgatcag aagcgccagg tagaccaagg aaagggcaaa ggaccacgag taggcgaact
      601 caccggcgag ggtcgacgcc acgttgccgc catagatgga gtagctctcg aggaaggcga
      661 acaccgcccc cgatccggca gccacgatcg aaacctgacg tccgaggagc atcgaccgcc
      721 ccagaaagta ggcggctggc ggaatcccga gaaggccgat gacggtgacc actttgaaag
      781 cgaccccgta gggcaggacc acgtcgagga ggacgatgac cagtgaagga aggggaaagt
      841 agaagtagaa ggcgggaaag cccgcaaacc aatcgttaga ccagccgagg atcctgccgt
      901 ggggaagcag attgtctctg agataggccg gcccaaagac gtgtgccccc atgtccccac
      961 cggtgggagt ggttgcagaa aggatccacc cggggttgag gacgatcagg atcaccaatg
     1021 agatcccggt gagaatgagg aacgtcggcc acgggaagcc ctggccggcc ctcctttgga
     1081 gggtcatgtt cgatgctggc ggacgatctc caagacctgg gcgacgtcga tgtcaacacc
     1141 gtccttgttc caccacttct cctcacagtt gtgacaggcg aagaagcgga cttcggtacc
     1201 gtcgggcaag agatgctcga tctcgatcac atcaggttga gagcactggg ggcagtccat
     1261 atcgattcgt tgctgcttgg ggtcggcaca tgctacggcc cacccagact tacgacaacg
     1321 gtccctcctc cagtagtcga cctacgtaac ggcttcggcg ccttccgccc tcggtccagt
     1381 atgcgtacca atagggacca tgagggcagg agttgcatcc ggcctttcca catcgaaccg
     1441 actgtttacg caaccgaacc cggggatgat cttcagcggc cagagcgccc gccttctgca
     1501 actgggccct ggtgaggatt aggaggcgtc tcaggtcgtg ctcgtcgaga tccttgaccg
     1561 cgtcgaggag atcgcggtcg agagccatca cgcaaccgac aacggggcaa acagccgcgt
     1621 cacggtccgc ctgtcggtga gagtccaacc ttggggcggg atcatccgat cggcatggtc
     1681 ggcgcaaagg gagtatccaa acggcgcgga gtcgacgacg aggtcgtcta gccacaccgc
     1741 ggcttcaggg tacgagaacg tcatcagggc cgccgagggc gagccacacc gaacacagct
     1801 gagaaccatg gcgagcacgg tagaagtcgg ccggtcgcca ttcaaggact cacgccgcct
     1861 tgcctcggct cagtgctccc cgggtacaga gtcgagcctt ccgttgcggt gactaccaag
     1921 gtggccggaa cggcatcgac accgagctct ctgaagatct ccgggtctga ttcccagctc
     1981 agctcggaga agcccactgg cccgaatcgc tcgagcagga cagcacgcac cgcctcacaa
     2041 tcggggcacg cctcggatgt gaacaggtac acccccgggc cgagccggtt ctgcttcagc
     2101 tctgaaggcg accgctgggt cggcctccgc cgaaggatca gggagagcgc cattccgaca
     2161 cctactgccc cgatgacggc gatgagccgg acttcgacct cactcatcag ggatcggggc
     2221 ggcaatgccg aggccgatcg agccgccgag cgtggtcgac caggtcgcga ccatggggcc
     2281 atcaccgcca atcgtgtaac cgagcactgg atcaggtgag atctcgatct ctgcgacttc
     2341 gccggcaggt accgagatcg tctccgagcc tgagcccgag cccaatgccg tcatcacggc
     2401 gctcacgtcc tcgagacctg gattcaggag tgcgagccat ccggatccac ctgccacgga
     2461 accgaccccg ggcaggagga gcaccgaagc caccgacgtg gttcccgaag tcagtgccac
     2521 gcctgtcgcg ctcgagatcc ggagaaatgc cccgacaggt gcggtcgtga ccactctcac
     2581 cgccgaagcc accggggaaa ctgccccgac gtcgaccacc gcctgtcccc ttgctgggat
     2641 ctggcccgac tccaaagcct caaccaatcc atctggccca tagaaatcaa tctgatattc
     2701 gacgtcagag tcacgggcgg caatcaccac ctgtctcgac tcgaggccat gaggaaccgg
     2761 gatgaaccag tcctgggcgg gggcgacggc atcccagatc gcgttgtcag ttccgatgcc
     2821 gagtcgacca actgccagga ccccacctga ttcgacttcg atggcgactg tcatccattc
     2881 ccgtcccgga agaatctccg ccagatccac gagaaccgtg gaacgggccg ggacgatcac
     2941 cgtgtccagg gcttcgctcg actccagtcc agcatcagac aagaccacga ggttgacgat
     3001 ggcctcaccc gcgtacgggt tcatgagctg aacctcgaaa cgctgatcgc taagcgtgga
     3061 accaccaccg agaatcaagt caccacccgc cgcgaccggg caggtatcca tcgccaggga
     3121 gtccgcgccg accaccactg aggccgcagc tgatgcgacg gcgggtagct ccaagagtgc
     3181 ccctcccacc ccaaccgcag cgatgtcggc gattgcgatc gaggagccgg tcgcggcgtt
     3241 ggtttcgaac tcaacggagc caaccgtggc accagccgca aacgcagtta gtgctccaga
     3301 tccatccaca gaagagacaa cgttgatgtt ggtcgctcgc ccgcttcctt cttcgacagc
     3361 gcacaccgcc attgccgggg tgtctaccgg agcggctggg gcgggctggg gtgatcctgg
     3421 tgcaggcagt gcaacggcac ccgccgccaa gacaacgacc atgagagtgc ggatcaaggc
     3481 tttcaacgcc gtaaccgccc aaccaccact gcggcgaaag ccgcaacgag aagggcaatc
     3541 gcggcgatcg acaacgcgag ccccgtcgga tccggtcggt agcgcccgct cccctgatcg
     3601 cctgcaacgg acacggccca gtcggcggcc tgtgggtcgg gtgaccatcg ggggtccgca
     3661 ttgatcgaga gcctgatgcg ggcgctggtg atatctccac cgaagcctgt acccacccgc
     3721 gaccaggtcg tcgccgaccc gctcgccatc gccatcgaat tcgggtttcc gtagatgcga
     3781 gccgtcggat cgagtggtgt tgggaccaga tccaactgaa cggcgaggac ctggtcgagg
     3841 taagtctccg gtccctcgat gacaacccaa tcgattgaaa actcggccag gctcgccccc
     3901 gggcgcagat cagcaccaga agccatttga tcgatcgcgg cggagagctt ctcgtctcca
     3961 ggaagcggtt caggaagcca cgcctcgtcc tgggtcatcc gggagccatc aatcagccga
     4021 taccaaagcc caggacccga acgggtctct ccggggatca ttgctcggtc ctcggataga
     4081 acgaggatcc ggcccgggcc ctcatcgccg gccaacgtcg cggcgaatcc gtacttcccg
     4141 ttgatgtcgc ccgacggaag accgagccgc ccgtccgcca tggagccgac cgaaagtatc
     4201 aaaaccgcga tcgcggccgc tgcggggagc acccgcttcg gctcgaggga aagacgatcg
     4261 agggcagacg ccaccacgat ggcagccccg aacgaggaga tgatcaggag agcctcttcg
     4321 tacccgggac ctcccaatgg aacgtccaac aagaccaagc ttgcgagact caggatcccc
     4381 ccggtgagag caaggcgtcc gatcccgttg tcgagaaaca agaggggtac gacaccgatg
     4441 atcagcagga acggccacga actgtctacg ccgaggccga gacggcgatt ggcgtcgatc
     4501 agccaccccg ggtcgccaaa aaggaacggc aacgcgacca ggatgccgac gaggcctgcc
     4561 acggccagcc acatgcgcga tctgtcgtcg ccgagagcac gtgccaggag aacggtgatc
     4621 aaggggacca cgaccagaac cggtgacatc gcgccgagga ggaggctcca ggcgatgacc
     4681 caggcgaatc gggacagcgg tttgccttcg ctgtcgttgg gatggacaaa gaccgatcgc
     4741 accgcccacg gaagcaggga tgccgcgatc aggcctagcc aggaaccggc tccaacaagg
     4801 actgcagcgc ccggcccggc gagtaggacg agaccggaca aatagcgccc gggcccacgc
     4861 agtccgaggc gcccggagag ccggcccatg ccgaggactg ccatgaaggc cgctcccacc
     4921 gtgatcagga gccgggccgc gccctcggcc ccgaaccaca ccacagacgc caacccggtc
     4981 atcccggtgg acggatgaac agccccaggg gagcccagac cggagtcgtt ccaacctcca
     5041 aagaaccgtg acagcgcgac tgaaggcggt tcgaagtcaa agctaaagcc tgcattggga
     5101 acaccggtga agatgatcga acgcatggcg accacggcca gcgcggcggc aatcaccgcc
     5161 ccccatatgc cggggctgga ccagattcgc ttcgtgcccc gggatagagc cttgtcgtcg
     5221 tcgaacatga acagcagtcg ctcggtgagc tcagcgccca agtcgcggag cctgaccgac
     5281 ccacgagcct ggaaacgaaa cagctcttcg tcgccaaaag ctcggaccgg cttgaacttg
     5341 cgccttgcag cgatcgtcga aggaaggtga accaggttcc agccccagga ggccacgtgc
     5401 ttcgctgcag gcctccagcg caacagaacg aggttgaaga gagaatcgac gatgcccacg
     5461 aacagctgat aaggaatgac ccaggccaac gtgatcggtc ggtacgcctt gagcattgcc
     5521 ctcatgcggc cggcttgctc tcgccaccca cgtccggcct gtcgacaccg gccctggtgg
     5581 aaaacctcag aagaagggac cgtgatcacc cggccaccgg ccagcctgac tcgttgcgag
     5641 aagtcgagac cggctgccac gggctccagg aggtcgtcga ggccttggag gccctgagca
     5701 aggtctcggc gcaccagcat cgagacggac gacacgaacg cgacttcccg gacgacgtcg
     5761 tattgctgga ggtcgatctc gccttcgtcg aggcccgaat acggctcgcc gaagacatcc
     5821 gtggcgctac ctatcgactc gagctcatcc cgagtgccgg caaccaggag cttggagcca
     5881 ccgagcgaag cgtcgttgcg ctccacctcc gacacgagcg cggccaacgc gtcggggcgg
     5941 ggccgggcat ctgagtggag gatccacagg tactcgacat cacttgaagt cgatgcgatc
     6001 gccgcctcga gggtcaccga acggccgatt ccgtcgggaa cctccaaccc ggagtccccg
     6061 acgacgatca cctcctcgac cgagtcatac acttggcggt cgacagtggc cagcgctgct
     6121 tccagatccg ctccttcgac cagggtgacc accaccttga tcttgggcgc gctcgcggaa
     6181 gggtcagttg tcgattcctc cgccatcagc ggagtcgagg catcggtcat gcggtgggtc
     6241 attgtgaacc gggtggcgac gctgagcaat atcgagctct gccgatgaga gtca
//
LOCUS       DBNHXU010000342         1630 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3695479, whole genome shotgun sequence.
ACCESSION   DBNHXU010000342 DBNHXU010000000
VERSION     DBNHXU010000342.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1630)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1630)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1630
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3695479"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="AC161X_08440"
     CDS             complement(<1..521)
                     /locus_tag="AC161X_08440"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR4989619.1"
                     /translation="MSVSVTFLGGLGEIGRNCAAVEIDGKIALIDCGLMFPEEDMLGV
                     DLVFPDWSWLVERVADVECVIVTHGHEDHVGALAYFLKVINVPVYGTRLSMELARGRI
                     EELGVEADLRVVEVKKWIDHGPFKFQLIPVSHSVPEAAAVALDTPEGVIVHSGDFKLD
                     PTPIDQIPTDLPA"
     gene            complement(518..1411)
                     /gene="dapA"
                     /locus_tag="AC161X_08445"
     CDS             complement(518..1411)
                     /gene="dapA"
                     /locus_tag="AC161X_08445"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006172785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HIR4989620.1"
                     /translation="MSPTPAAPFGHVATAMITPFTGSGVVDHEKAWRLARYLAEHGSQ
                     ALVVTGTTGESPTLSAAEKIALYKTVVDAVKEKSTFVIAGTGNYNTAESIELTEKAGD
                     LGCHGVLAVTPYYNKPGQSGLIAHFSAIADVGIPVMLYNIPGRTGRLIEVDTLARLSE
                     HDNIVAVKDAVENIDFTSQTVNRVPGLPVYSGQDSHTWPMMAVGAVGVVSVISHLAGP
                     TVAAMVKAAIAGDTAGARRLHHELLPLCEACFLETNPAPVKGAMNRLWEPVGDVRLPL
                     VAASEETLIAVEKAVGAIQGL"
     gene            1449..>1630
                     /locus_tag="AC161X_08450"
     CDS             1449..>1630
                     /locus_tag="AC161X_08450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015745862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989621.1"
                     /translation="MELRLPAPSLIVLIGPSGSGKTTWANEHFRSGEIVSSDQLRAVV
                     GTGEDDQRASPVAFDIL"
BASE COUNT          311 a          540 c          493 g          286 t
ORIGIN      
        1 aaggcgggaa ggtcggtcgg aatctgatcg atcggagtcg gatcgagctt gaagtcgccg
       61 gaatggacaa tcaccccttc aggcgtgtcc agcgccaccg cggcggcctc gggcaccgag
      121 tggctaaccg ggatcagctg gaatttgaac ggcccgtggt cgatccactt cttcacctcg
      181 accacccgaa ggtcggcctc gactcccagc tcttcgatcc ggccccgggc caactccatg
      241 ctgagtcggg ttccgtagac cgggacgttg atcaccttca agaagtaggc gagagcccca
      301 acatggtcct cgtggccgtg ggtgacgatg acacattcga cgtcggcgac ccgctccacc
      361 agccacgacc agtctggaaa gacgagatcg acaccgagca tgtcctcttc gggaaacatg
      421 agcccgcagt cgatgagggc gatcttgccg tcgatctcga cagcagcgca gttgcgaccg
      481 atctccccca aaccccccag aaaagttacc gagacactca taagccctgg atcgccccca
      541 ccgccttctc gactgcgatc aaagtctcct cggatgcagc gaccaacgga agcctgacat
      601 ctccgaccgg ctcccagagc cggttcatcg cgcccttgac cggtgcgggg ttggtctcga
      661 ggaagcaggc ctcgcaaaga ggcagaagct cgtgatgcaa ccgccgcgcc ccggcagtgt
      721 cgcccgcgat ggcggccttc accatcgcgg ccacggtggg gcccgccagg tgtgagatca
      781 cggagaccac cccgactgcc ccgaccgcca tcatcggcca ggtgtgagag tcctgacccg
      841 agtacaccgg cagcccggga acacgattga ccgtctggct ggtgaaatcg atgttctcca
      901 cggcgtcctt gaccgcaacg atgttgtcgt gctcactgag ccgggcgagg gtatcgacct
      961 cgatcaggcg cccggtgcga ccggggatgt tgtagagcat caccggtatc ccgacgtcgg
     1021 caatggcgct gaaatgagcg atcaggcctg attgcccggg cttgttgtag tacggggtca
     1081 ctgcgagcac accatggcaa ccaagatcgc cggccttctc agtcaactcg atcgactccg
     1141 cggtgttgta gttcccggtt cccgcgatga cgaaggtgct cttctctttc actgcatcga
     1201 ccacggtctt gtagagggcg attttctcgg cggcactgag agtcggcgac tcgcccgtgg
     1261 tgcccgtcac caccagcgct tgtgatccgt gctcggcgag gtaccgggcc agacgccatg
     1321 ccttctcgtg atcgaccaca cccgagccgg tgaatggggt gatcatggcg gtggcgacat
     1381 ggccgaaggg ggcagcaggc gtgggactca ccacgtcagg ttaccgatcg cccgactaga
     1441 acagccccat ggagctgcgt ctccccgccc cgagcctcat cgtcctcatc ggaccctccg
     1501 gttccggaaa gacgacctgg gcaaacgagc actttcggtc cggcgagatc gtctcctctg
     1561 atcagctacg ggcagtggtc ggcaccggcg aggacgacca acgcgccagc ccggtcgcat
     1621 tcgacatcct
//
LOCUS       DBNHXU010000343         1519 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5488484, whole genome shotgun sequence.
ACCESSION   DBNHXU010000343 DBNHXU010000000
VERSION     DBNHXU010000343.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1519)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1519
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5488484"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="AC161X_08455"
     CDS             complement(<1..1385)
                     /locus_tag="AC161X_08455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HIR4989622.1"
                     /translation="MLGLTVFLGLALLLIGRRDASNRAPLAAVTLAALGASLFAPLWL
                     LDQGDQIALFIGSITPPMLISFWGSLAGVATLAFLATFPDGKWTPSWTRWLVVAAAVS
                     GLASFFFPRTFFDPRVWPSSLQFAWLVGLPIAAIFAQLVRSRQPDFVKTTKPIVISLV
                     TALGAFLVLWILQPELTADVLDLIVVTPRLKAVYALNVLVLLTIAVFMFPVSVSVAIV
                     RHRLFDLDLLVNRALVYGTVTALVGIGFLALSAGVAAVAGGVSDLTITWQGGLGGVFL
                     GTVLVLVFQPLRTRVQRGVDRRFYRERYDARQVIDDFAGEAARLVDPATLEAELVSTV
                     ERALRPKSVQLHTGPFSKATRKALASGAAIDLEGVAPSKGLAPFSANGTAILVPLVAG
                     GSLTGVLDLGDRASDSRYSTLDLELLDRLAQTAGPALQLAYEVQMREKDAQNKERAAH
                     ELELARKIQQGL"
BASE COUNT          328 a          449 c          501 g          241 t
ORIGIN      
        1 aggccttgtt gaatcttgcg ggccaattcc agttcgtggg cggcgcgttc cttgttctgg
       61 gcgtccttct ccctcatctg cacttcgtag gcgagttgca gtgccggccc tgccgtctgg
      121 gcgaggcggt cgagcaactc gaggtcgagt gtcgagtaac gggagtctga ggcccggtca
      181 ccaaggtcaa gcaccccggt tagggagcct ccggcaacca gtgggacaag tatcgccgtc
      241 ccattggcgc tgaaaggagc gaggcctttg ctcggcgcca caccttcgag atcgattgcg
      301 gcgccggagg cgagtgcctt cctcgttgcc tttgagaatg gcccggtgtg gagctgtacc
      361 gacttcggcc gaagggccct ttccaccgtg gagaccaatt ctgcctcgag cgtggccggg
      421 tcgacgaggc gggccgcctc cccagcgaag tcgtcgatca cctggcgagc gtcgtagcgc
      481 tcccggtaga accgccgatc aactcctcgt tgaacccgtg ttcgcagtgg ttgaaagacc
      541 aggaccaaga ctgttccgag gaatactcct cccaggcctc cctgccatgt gatggtcaaa
      601 tcggaaaccc ccccggccac cgccgccact cccgcggaga gcgcgagaaa gccgattccc
      661 accagggccg tgaccgtgcc atacaccaag gctcggttga ccaggagatc gaggtcgaag
      721 aggcgatggc ggacgatggc caccgagact gacaccggga acatgaagac tgcgatggtc
      781 agcaacacca ggacattgag ggcataaacc gccttgaggc gcggagtaac cacgatgagg
      841 tcgagcacat cagcggtgag ttctggctgg agaatccaga gcacgagaaa tgcgccgagg
      901 gcggtcacca acgaaatgac gatgggcttc gtcgtcttca caaagtcagg ctgacggctg
      961 cgaaccaact gggcgaagat cgccgcgatc gggagcccga ccagccaggc gaactggagg
     1021 gatgagggcc aaactcgcgg atcgaagaag gtgcggggaa agaaaaagct ggccagcccg
     1081 ctgaccgcag ccgccaccac caaccatctg gtccaggacg gtgtccactt gccgtcgggg
     1141 aacgtcgcga ggaaggcaag agtggcgacg ccggccaggg acccccagaa gctgatcagc
     1201 attggcgggg tgatcgaccc gatgaagagg gcaatctggt caccctgatc gagaagccat
     1261 agcggggcaa agaggctcgc tccaagtgca gccagagtca cggcagccaa tggagctcga
     1321 ttggaggcat cgcgccggcc gatgagaagc aatgccagcc cgaggaacac ggtgagtccg
     1381 agcacatgga gcagacgttg agtggtctcg acgaattcgg gctggaacag aacggcgagt
     1441 tcttgttcga tgcgagggtc gaccgccgcc ggaccctgcg cgatcaagcc atctggtggg
     1501 tcgtcaaaca gtgtgacca
//
LOCUS       DBNHXU010000344         2072 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5091757, whole genome shotgun sequence.
ACCESSION   DBNHXU010000344 DBNHXU010000000
VERSION     DBNHXU010000344.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2072)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2072
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5091757"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1262
                     /locus_tag="AC161X_08460"
     CDS             <1..1262
                     /locus_tag="AC161X_08460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989623.1"
                     /translation="RSGDYESAIALAGALRWFWVIKREVSEGTEWIERTLTKREGASD
                     RVVARALNGAGLLAIRRLDFARAATALVDGLDLYRRMGDGPGEARQVYLLGVLAWFED
                     DLDKAERLITEALDLSRTHDVAWGVAWSLAVKGTMARLRGDLVGARRDMLESHQVFLD
                     RMGTLDIGWSLLRLGALARDEGRYSEAEDRYSSGRSLLLKAGDTLGLAHADAGLGAMS
                     WLAGDHDHALDLYRSVLEGFSLSEEASNNLFELKTMIQGNPSTAELQEIVETNRNRAV
                     RIEGHAGARAALAEYLYHVGKTAFRQGQIERSKTALAESLRLADAAGDKRGAAIAVAG
                     LAVSAHTLGDNETAARLFGLAEWMASEGQVRIWPPPDEPDYSSRVGSVRAALGSGFAR
                     STMDGEALTVDKAIELAFPASDLTDPS"
     gene            complement(1259..1828)
                     /gene="cobO"
                     /locus_tag="AC161X_08465"
     CDS             complement(1259..1828)
                     /gene="cobO"
                     /locus_tag="AC161X_08465"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007860962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HIR4989624.1"
                     /translation="MSDPTTPPSEPPPAATVREPSLVLVNTGEGKGKSTAAFGTAIRA
                     VARGWRVGVIQFLKSGDWHVGEETVGRQLGIDWWALGDGFTWDSKDIDESEAIAREAW
                     SVAKEKIDSGEYDLVVLDEITYPINWGWIALDAVLEVIAARPANVNVILTGRDAPDEL
                     IELADTVTEMVKVKHAFDRGVMARRGIDF"
     gene            complement(1821..>2072)
                     /locus_tag="AC161X_08470"
     CDS             complement(1821..>2072)
                     /locus_tag="AC161X_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989625.1"
                     /translation="VLEIVDDLRLSDGLTVVTALHDLTLAGRFAGRVVMLASGKVVAD
                     GSPDDVLTEELLRSHYGAGIRVVDNGEGPTVIPTRNRRV"
BASE COUNT          372 a          669 c          659 g          372 t
ORIGIN      
        1 tcaggtccgg cgactacgag agcgcgatag ccctcgccgg cgccttgcgc tggttttggg
       61 tcatcaaacg cgaggtctcc gagggcaccg agtggatcga gcggaccctg acgaagaggg
      121 aaggcgcatc ggaccgcgtc gtggctaggg cgctcaacgg tgccgggctc ttggccatca
      181 ggcgcctcga tttcgcacga gccgccaccg ccctggtcga tggtctcgac ctctaccgga
      241 ggatgggtga tggcccgggc gaagccagac aggtctatct cctcggggtc ctcgcctggt
      301 tcgaggatga cctggataag gccgagcgcc tcatcaccga ggccctggat ctgagccgca
      361 cccatgacgt ggcctggggc gtggcatggt ccctcgctgt gaaggggacg atggcccggc
      421 tgagagggga cctcgttggg gcgcgcaggg acatgctgga gagccatcag gtcttcttgg
      481 accgcatggg caccctcgac atcggttggt ccttgcttcg actcggggcc ctggcgcggg
      541 acgagggcag gtacagcgag gccgaggaca gatatagctc cgggcgaagc ctcctcctga
      601 aggcaggaga cactctgggc ctggctcacg ccgacgccgg actgggagcg atgtcctggc
      661 tggcgggtga ccatgaccat gcccttgacc tctacaggtc ggtcctggag ggtttcagcc
      721 tgtccgaaga ggcttccaac aacctcttcg agctgaagac gatgattcag ggcaatccat
      781 cgactgcgga acttcaagag atcgtcgaga ccaaccggaa ccgggcagtg cgaatcgagg
      841 gtcacgcggg cgcccgtgcc gccctcgccg agtacctata tcacgtcggc aagaccgcgt
      901 ttcgacaagg gcagatcgag aggtcgaaga ccgccctcgc ggaaagcctg agactcgccg
      961 atgccgccgg cgacaagcga ggcgcagcca tcgcagttgc cgggcttgcg gtctccgccc
     1021 acacactcgg cgacaacgag acagcggctc gccttttcgg acttgccgag tggatggcca
     1081 gcgaaggcca ggtccgaatt tggccgcctc cggacgagcc ggactattcg tcgagagtcg
     1141 gctcagttcg ggcggccctg ggttctggat tcgcccgctc caccatggat ggggaggcct
     1201 tgacggtgga caaggcaata gagcttgcct ttccggcttc tgatctcact gacccgagct
     1261 agaagtcgat tccgcgccgc gccatgactc ctcggtcgaa ggcgtgtttc accttgacca
     1321 tctcggtcac cgtgtcggcc aactcgatca actcgtcggg ggcatcacgt ccggtgagga
     1381 tcacgttcac attcgccggc cgggccgcga tcacctcgag gacggcgtcc agtgcgatcc
     1441 aaccccagtt gatcgggtag gtgatttcgt cgagaaccac gaggtcatat tcgccagagt
     1501 cgatcttctc cttcgcgact gaccatgctt cgcgtgcgat cgcttccgac tcgtcgatgt
     1561 ctttcgaatc ccaggtgaag ccgtcgccca gcgcccacca gtcgatcccg agttgccgcc
     1621 caaccgtctc ttcaccgacg tgccaatcac ccgatttgag gaactggatc actcccactc
     1681 tccaacccct ggccacggcg cggatcgcag tgccgaaggc cgcagtggat ttccctttgc
     1741 cctcaccggt gttcaccagg accagggagg gctcgcgcac ggtcgccgcc ggtggtggct
     1801 cgctgggggg cgtcgtcgga tcagacacgc cggttcctgg tgggtatcac agtcgggcct
     1861 tccccgttgt caaccactcg aataccggca ccgtagtgag atcgaagcag ctcctcggtc
     1921 agcacgtcat ccggggatcc atcggcgacg accttccccg acgcaagcat caccacccgc
     1981 ccggcgaacc ttccagcgag ggtgagatca tggagtgcgg tgacgacagt gagcccatcg
     2041 ctcaacctca ggtcgtcaac gatctcgaga ac
//
LOCUS       DBNHXU010000345         1573 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2313993, whole genome shotgun sequence.
ACCESSION   DBNHXU010000345 DBNHXU010000000
VERSION     DBNHXU010000345.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1573)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1573)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1573
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2313993"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            112..891
                     /locus_tag="AC161X_08475"
     CDS             112..891
                     /locus_tag="AC161X_08475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014228.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - histone deacetylase activity,
                     NAD-dependent [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family NAD-dependent protein deacylase"
                     /protein_id="HIR4989626.1"
                     /translation="MTDISLAIEALRPAEQILVFTGAGISTESGIPDFRGPDGLWTKV
                     DPEDFHIDRFRASSDLRKRGWRMHLDGELWGARSQVEPNHGHLAIKRLADAGRLAGVV
                     TQNVDGLHHRSGLDDDMVAELHGNVRESHCLDCDRTWPTEEVLGWVEAGDEDPTCPDC
                     GGMVKTRTVMFGEMLPDEEMRKAMIFLAIADALLVVGSTVAVWPASDVVFRAAHRSLP
                     IVIINRGETEADNLATAKVDAGIGEVLPDLVDGLLAAAPPS"
     gene            963..>1573
                     /locus_tag="AC161X_08480"
     CDS             963..>1573
                     /locus_tag="AC161X_08480"
                     /inference="COORDINATES: protein motif:HMM:NF014790.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR4989627.1"
                     /translation="MAEYAPRLDDVNFALNHVADLEAVSKLNGYQHADPATVAGILEE
                     AGRFFSEVIAPLNQIGDRQGSVLTDDGTVRTPDGFKEAYRKYVEAGWASVHIPKEWGG
                     GGLPILVGVVIEEMFKSANIAFSLCPTLTHAAVEALRVHGSEELRGTYMEKLVSGEWS
                     GTMNLTEPEAGSDLGGLRTKAVPQDDGTYRLFGTKIFITWGDQ"
BASE COUNT          299 a          482 c          510 g          282 t
ORIGIN      
        1 ccgagggtca tcatctcggt ctccggcagt gattcgccgt tgcgctggtc ttcagtcatc
       61 ggaggagcct aaaacgaagc ggtaaccggc aagtcagccg ataatcgccg tgtgactgac
      121 atcagcctcg cgatcgaggc actccgcccc gcagagcaga ttctggtgtt caccggcgcc
      181 ggcatttcga cggaatccgg tatccccgat tttcggggtc ctgacgggct gtggaccaag
      241 gtggatcccg aggactttca catcgatcgc ttccgggcca gttccgacct gcgaaagcgc
      301 ggctggcgga tgcatctcga cggtgagcta tggggggcca gatcacaggt ggagccgaac
      361 catggccatc tcgcgataaa gcggctggcc gacgcgggac gcctcgccgg tgtcgtgacc
      421 cagaacgtcg acggccttca ccatcgatcg ggattggacg acgacatggt cgccgaactg
      481 cacggcaatg tcagagagag ccactgtctg gactgcgacc ggacctggcc caccgaagag
      541 gtcctcggct gggtagaagc gggcgacgaa gacccgacct gtcccgattg cggcgggatg
      601 gtcaagacga gaaccgtcat gttcggcgag atgctccccg atgaggagat gcgaaaggca
      661 atgatctttc tggcgatcgc cgacgccctt ctcgtggtcg gctcgacggt tgcagtctgg
      721 ccggcgtcgg acgtcgtctt tcgtgcggcg caccgttcgc tgccgatcgt gatcatcaac
      781 cgcggggaga ccgaggccga caacctggcc accgccaagg tcgacgccgg catcggagag
      841 gtgctgccgg atctcgtcga tgggctgctc gccgccgcac ccccttcgta attggctgac
      901 aacaggggtc agatacggac ctcctgaacc caatttggca ggggtggttt agcctttgag
      961 ccatggccga atacgcccct cgcctcgatg acgtcaactt cgcactgaat cacgtggccg
     1021 acctcgaagc ggtctcgaaa ctgaacggct accagcacgc tgaccccgca accgtcgccg
     1081 gcattctcga agaggccggg cgcttcttct ccgaagtcat cgctcctctc aaccagatcg
     1141 gggatcgcca ggggtcggtc ttgaccgacg acggaacggt gcgcaccccc gacggcttca
     1201 aggaggcgta tcggaagtac gtggaagcgg ggtgggcttc cgtccacatc cccaaagagt
     1261 ggggaggcgg cgggctgccg atactggtcg gagtggtcat cgaggagatg ttcaagtccg
     1321 cgaacatcgc ttttagcctc tgtccgaccc tgacccatgc ggcggtggag gcccttcgcg
     1381 tccatggctc ggaagagctc cggggcacct acatggagaa gcttgtgagt ggggagtgga
     1441 gcggcaccat gaatctcacc gagcccgaag ctgggtcgga tctcggtgga cttcgcacca
     1501 aggcagtgcc ccaggacgac gggacgtacc ggctcttcgg caccaagatc ttcatcacct
     1561 ggggcgatca gga
//
LOCUS       DBNHXU010000346         1692 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4694837, whole genome shotgun sequence.
ACCESSION   DBNHXU010000346 DBNHXU010000000
VERSION     DBNHXU010000346.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1692)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1692)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1692
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4694837"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..534)
                     /locus_tag="AC161X_08485"
     CDS             complement(91..534)
                     /locus_tag="AC161X_08485"
                     /inference="COORDINATES: protein motif:HMM:NF025204.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4193 family protein"
                     /protein_id="HIR4989628.1"
                     /translation="MANEELDELIEELPDEVDVPEEELDDALVEELEEVELVPEFEEE
                     FVAEEEVEDFDTDDEEEVHDDETTEALEELESEELQLMDEEVGETLLVDEVEELRAIR
                     REELTMNVDAQLQRSDEFVCQSCFMVKRTSQLANRKKMLCSDCAY"
     gene            complement(583..1467)
                     /locus_tag="AC161X_08490"
     CDS             complement(583..1467)
                     /locus_tag="AC161X_08490"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001727 - lipid kinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol/lipid kinase family protein"
                     /protein_id="HIR4989629.1"
                     /translation="MTKWLVAVNGKAGRLTTDPTLIADVVRTAGLDAEIVAPSSPSEM
                     ADALVAGAGRGFEHFAVAGGDGTVNLAVNALLPLDLGFSPVIGVLPVGTGCDLLRTFG
                     LPQDLAGAAQHLATDSVYPIDVGALEGEWGLRYFVNVAQAGVGAAAAQTAVKIARWLG
                     PVRYPMAFGGRLPRFPRAQITLTTEKKVVESEALAVIFANAQFFAGGWNVAPKATLVD
                     GVLDIQIFDVKKGQAAALVPKVIKGTHLREPGVRRLSVSEFSLVTEPSWPVEADGDLI
                     GNTTVNGRVIPAAVRLKI"
     gene            complement(1467..>1692)
                     /locus_tag="AC161X_08495"
     CDS             complement(1467..>1692)
                     /locus_tag="AC161X_08495"
                     /inference="COORDINATES: protein motif:HMM:NF019758.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989630.1"
                     /translation="PDPGFAMLAHIWASGTDFDDLPTRGMAPGDFVRVSRQLVDLLRQ
                     LREAAPGMREEAVQALKSVDRGVVAAQGVG"
BASE COUNT          313 a          574 c          480 g          325 t
ORIGIN      
        1 aggacccgat aaccctggac cgtgaccgcc gcaacgattt ccgagatgtt ctcccgctcg
       61 ttgtatgtgg ggattacgac cgttacccga tcagtaggca cagtcggagc acagcatctt
      121 cttgcgattg gcgagctggc tggttctctt gaccatgaaa cacgactgac agacgaactc
      181 gtcggagcgc tgaagctgag cgtcgacgtt catcgtcagt tcctccctgc gaatcgcccg
      241 caattcttcg acctcgtcga caaggagagt ctctcccacc tcttcgtcca tgagctgaag
      301 ctcctcgctt tccagctctt cgagcgcttc ggtggtctcg tcgtcgtgca cctcttcctc
      361 gtcgtcggtg tcgaagtcct cgacttcctc ctcggccacg aactcttctt cgaactcggg
      421 gacgagctcg acctcttcga gctcctcgac gagcgcgtcg tccagttcct cttcaggaac
      481 atcgacctcg tcgggcagct cttcgatgag ctcgtctagt tcttcattgg ccattttgat
      541 gtttcaggtg cctcgggggc gggcagtata agtcggcgcg gctcagatct tgagacgcac
      601 cgcggccggg ataacccggc cgttcaccgt ggtattcccg atcaggtcac catcggcctc
      661 gaccggccag gatggctcgg tcaccaagct gaattccgac accgaaagac gtcgaactcc
      721 gggctcacgc agatgtgtcc ccttgatcac cttgggcacc agtgcggcgg cctgaccctt
      781 cttgacatcg aagatctgga tgtcgaggac accgtcgaca agggtcgcct tcggagccac
      841 gttccagccg ccggcgaaaa actgggcgtt ggcgaatatg accgccagag cctcggactc
      901 gaccactttc ttctccgtgg tgagcgtgat ctgcgccctg ggaaagcggg gcaggcgccc
      961 accaaacgcc atcgggtagc ggacagggcc tagccagcga gcgatcttca ccgcagtctg
     1021 cgctgcggcc gccccgacgc cagcctgagc cacgttcacg aagtagcgaa ggccccactc
     1081 gccttccagc gctcccacgt cgatcggata cacggagtcc gtagccagat gttgggccgc
     1141 cccggcaaga tcctgaggta atccaaaggt tcggagtaga tcgcagccgg tccccacggg
     1201 caggactccg atcaccggcg agaagcccag atcgaggggc agtagggcgt tcaccgccag
     1261 attcaccgtt ccgtcgccac cggcgaccgc aaaatgctcg aatcctcgac cagctccggc
     1321 gaccagtgcg tcagccatct cgctgggcga ggagggtgcc acgatctccg cgtccaaccc
     1381 agcagtcctg accacatcgg cgatcaatgt gggatcggtt gtgagacggc ccgccttccc
     1441 attcacggca accagccact tcgtcatcaa ccgactccct gcgcagccac cacgccccgg
     1501 tccaccgact tcagcgcctg cacggcctct tctctcatcc cgggtgctgc ttccctcaac
     1561 tggcgcagca ggtcaaccaa ttgccgagag acccggacga agtctccggg ggccatgccc
     1621 cgggtcggca ggtcgtcaaa gtcagtgccc gaggcccaga tgtgggccaa catcgcgaaa
     1681 ccggggtcgg gt
//
LOCUS       DBNHXU010000347         2400 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4099618, whole genome shotgun sequence.
ACCESSION   DBNHXU010000347 DBNHXU010000000
VERSION     DBNHXU010000347.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2400)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2400
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4099618"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(149..2023)
                     /locus_tag="AC161X_08500"
     CDS             complement(149..2023)
                     /locus_tag="AC161X_08500"
                     /inference="COORDINATES: protein motif:HMM:NF021588.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta-hydrolase family protein"
                     /protein_id="HIR4989631.1"
                     /translation="MPRTSVHQGLATGLSLIAARGVTKLVDIPISAVIPAEAGLVTHL
                     TGRAVMGGAGAALAALPVEDGETMWVSTARTGGQIVRAAAIGGAIYDVGTAVRRRYGS
                     DSPIQPLIISASALAGVLYTARRRRTARQEAVGKWPIVQEVTIPTAVGTAIAVNNFGR
                     VVGWGYKASRDRWINYLGPGWSKNVMGRVINAGLWAGAGVAAYRAGVGKLTKSNDKIE
                     AGYATPPVAPEVSGSADSVSTFEELGLQGRRFVVDVLTPEIIKETLGEPAKTQPIRTF
                     IGFNTEPIYSTGRAELALEELDRTGAFDRSYLLLVSPTGTGWVDHTVVEAAELMTRGD
                     IATCCIQYARSPSFLAVQQVALGRAQFRLLLWGIRERLRERAPEKRPKVLIFGESLGA
                     WTSSDVVMHQGITGFDHYGIDRALWFGLPWLAKWSRSGMIRQTSESVPEGTVGVFDRH
                     EQLAALTPDQRDRMRAVILSHDNDPIALFGPDMLVRKPDWIGAEARRGVADSIEWTPI
                     VTFWQTAIDAANAMVTIPGDFQSFGHDYRADTARFVRDAYHLPATTDDQLERIEKALR
                     TLELERSERIKASHIADAPPAPAEREDGGRAVSAGVPLVAKKAKGANWRRSLQGKHRS
                     "
     gene            complement(2162..>2400)
                     /locus_tag="AC161X_08505"
     CDS             complement(2162..>2400)
                     /locus_tag="AC161X_08505"
                     /inference="COORDINATES: protein motif:HMM:NF013989.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HIR4989632.1"
                     /translation="VLGHFGSGVILCEGGPGINAQLAEVGAIDELNLTVAPLIVGGQS
                     KRVAQGATAHSPQEMELDQILAGDRSLFLRYRST"
BASE COUNT          451 a          780 c          718 g          451 t
ORIGIN      
        1 gccgcccgtc gccatcgact atcgctatcg gctgctggta gtaaagcggt gcccaaccgg
       61 gcaagacctt ttgatgtctc tcgtagagct cggcgtgctt tcccatcagc gcgaggttac
      121 cgagccatgt cgttggtcac accaaaactc agctgcgatg tttcccctgc aagctcctgc
      181 gccaattcgc gcccttcgcc tttttcgcaa ccagggggac gccagcgctg accgcgcggc
      241 caccgtcttc acgctcggca ggagctggcg gggcgtcggc aatgtgactt gccttgattc
      301 gctcggaccg ttccaactcc aaggttcgga gcgccttctc gattcgctct agctgatcat
      361 ccgtggtcgc tggcaggtga taggcgtcgc ggacgaaccg ggcggtgtcc gctcgataat
      421 cgtggccaaa cgattggaag tcgccaggga tcgtgaccat ggcatttgca gcgtcgatcg
      481 cggtctgcca gaaggtgacg atcggagtcc attcgatcga gtcggccacg ccgcgcctgg
      541 cctcggcacc gatccagtct ggctttcgca ccaacatgtc gggaccgaac agggcgatgg
      601 ggtcgttgtc gtgggaaaga atgacagccc gcatcctgtc ccgctgatct ggcgtgagag
      661 ccgccaattg ctcatgccgg tcgaaaacac cgaccgttcc ctccgggacc gactcagacg
      721 tctggcggat catgcccgac cgcgaccatt tcgcgagcca aggcagtccg aaccagaggg
      781 cccggtcgat cccgtagtga tcgaatccgg taattccctg atgcatgacc acatctgacg
      841 aggtccaggc gccgaggctt tctccaaata tgaggacttt gggccgcttc tcgggagccc
      901 gctcccgcag ccgttccctg atcccccaca gcaagagccg gaactgggcc cttcccaggg
      961 ctacctgctg caccgccaga aacgaaggcg aacgcgcata ttggatgcaa caggtggcga
     1021 tatcacctcg ggtcatcagc tcggcggcct cgaccaccgt gtggtcgacc caaccggtcc
     1081 cggttggtga taccaacaga aggtacgaac gatcgaaggc tccggtccga tcgagctcct
     1141 cgagggcgag ttcggctcgc ccggtcgagt agatgggctc ggtgttgaat ccgatgaacg
     1201 ttcggattgg ctgcgtcttg gcgggctcac cgagggtctc tttgatgatc tcaggcgtga
     1261 gcacgtccac tacaaagcgc cgaccttgca atcccagttc ctcgaaggtg gaaacactgt
     1321 ccgcgctgcc tgaaacctcg ggggcgaccg gtggtgtggc gtagccagcc tcgatcttgt
     1381 cgttgctctt ggtcagcttt ccaaccccgg cccgataggc ggcgacgccg gcacccgccc
     1441 atagtcccgc gttgatcacc cttcccatca cgttcttcga ccaaccaggg ccgaggtagt
     1501 tgatccagcg gtcccgactc gccttgtacc cccaaccgac gaccctgccg aagttgttca
     1561 cagcgatcgc ggtgccaacg gccgtcggga tggtcacctc ctggacaatc ggccattttc
     1621 cgaccgcttc ctgtctggcg gtccgtcgtc gtctggccgt atagaggacc ccggcaagag
     1681 ccgaggccga gatgatgagg ggctggatcg gtgaatcgga gccgtagcga cgtcgtactg
     1741 cggtacccac gtcgtagata gcgccaccga tggcggccgc acggacaatc tgaccgccgg
     1801 tcctcgcagt ggagacccac attgtctcgc cgtcctcgac cggaagagcg gcaagcgctg
     1861 cccccgcgcc tcccatgacg gcacgacccg tcaaatgtgt cacgagtccg gcctcggctg
     1921 ggatgaccgc ggaaatgggg atatcgacca acttggtgac accgcgcgca gcgatcaggc
     1981 tgagtccggt cgccaatccc tggtgcacgg aggtcctggg catcagcgaa ggaccgaacg
     2041 agtcgataaa taccgttgtc tccagtggtg aagacgatga cccggttacg gggttcaacc
     2101 accgagcgac ggagctgaca acatcgccaa gtcctgacat tcgcatggtc accctatcgg
     2161 atcatgtcga tcgatagcga aggaaaagag accggtcgcc ggccaggatc tggtccaact
     2221 ccatctcctg cggagaatgc gccgttgcac cctgggccac cctcttcgac tggccaccga
     2281 cgatcagggg cgcaaccgtc aggttcaact cgtcgattgc gccgacctcc gcaagctgtg
     2341 cgtttatgcc gggccctcct tcgcagagga tgaccccgga cccgaaatga cccagcacgg
//
LOCUS       DBNHXU010000348         2508 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5492234, whole genome shotgun sequence.
ACCESSION   DBNHXU010000348 DBNHXU010000000
VERSION     DBNHXU010000348.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5492234"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..2495
                     /locus_tag="AC161X_08510"
     CDS             123..2495
                     /locus_tag="AC161X_08510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012208081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HIR4989633.1"
                     /translation="MTALTVDGEQPLYRDPAQPVHERVEDLLERMTWDEKIAQLGSVW
                     VFQILDGSAFSAEKARDLLRNGLGQVTRVSGASSLDAVAAARVANSIQEHLINETRLG
                     IPAIVHEEICSGLMARESTVFPQAIGVANTWEPALAEAMADVVRVQMRSMGAHQGLSP
                     VLDICRDPRWGRTEETFGEDPFLVSKMGVAFVRGLQGEQMGEGVLATAKHFVGYGASQ
                     GGLNWAPAPIPAREMREVYLHPFEAVVKTADLQSIMNGYHELDGIPCGADRDLLTGLL
                     RNEWGFDGSVVSDYFSIRQLASYHHLTDSAVDAAAIALNAGLDVELPSTDCFGDPLLE
                     AIRSGSVSEETLDNAVRRVLGAKFVLGLFENPNVDPDNASSVVDAPFHRDLARQIARK
                     TMVLLKNNGVLPLKDDLSSIAVIGPNADVARNMFGDYTYPAHIESLMEMRHRENVFSI
                     PFPDDGQVDDPTPKGPTVVDALRDRFGEKVRYAPGCDIQGESRDGFEEAVALAAESEL
                     AILVMGDKAGLTEDCTTGEGRDRLSLDLPGVQEDLVRAVVGTGTPVVLVLVAGRPCGS
                     VLAHQSSGAVIAAGLPGQEGAAAIVDVLSGDFNPGGKLAMSHPRTVGQIPVFYGHKVS
                     GGRSHWKGDYVDGSARPLYSFGHGLSYTAFELSETTVAGGQAGWNDTVTITTRVSNVG
                     SRDGDEVVQLYVRDPEASVTRPVLELKAFKRVEVPAGESALLRFGLPIGQIGFYNRDL
                     DYVVEPGRVDLLIGTSSSDLVEIGFITVVPDPSGEPPRKVFDGRVEVEMT"
BASE COUNT          482 a          764 c          804 g          458 t
ORIGIN      
        1 tacgtaaccg acaccgcgat gatcctcgcc tataccaccg tggcgatgat tcccgccctt
       61 atcttctact tcatagccca acgccagatc gtgagtggtc tcacggcagg aggggtcaag
      121 ggatgacggc actgacagtt gacggcgaac agccgctcta tcgggacccg gcccagccgg
      181 tccatgaaag agtcgaagat ctactcgaac ggatgacctg ggacgagaag atcgcacagc
      241 tagggagcgt gtgggttttc cagatcctcg acgggagcgc cttctccgcc gagaaggctc
      301 gggacctcct gcggaatggc ctggggcagg tgaccagggt gtcgggggcg agcagtctcg
      361 atgcagtggc cgcggcgcgg gttgcgaatt cgatccagga gcacctgatc aacgaaaccc
      421 gcctgggcat tccggcgatc gtccacgagg agatctgctc gggattgatg gcgcgcgagt
      481 ccactgtctt cccccaggcg atcggcgtcg caaacacctg ggagccagca ctggcggagg
      541 ccatggccga cgtcgtcaga gtgcagatgc ggtccatggg tgcccaccag ggactttcac
      601 cggttctgga catctgtcga gacccgcgat ggggccgcac cgaggaaacc tttggtgagg
      661 acccattcct ggtgtcgaag atgggggtcg ccttcgtccg tggtctccaa ggcgagcaga
      721 tgggcgaagg cgtcctggcg accgccaagc acttcgtcgg gtacggcgcc tcacagggag
      781 gcctgaactg ggcacccgcc cccatcccgg cccgtgagat gcgcgaggtc tatctgcatc
      841 ccttcgaagc cgtggtcaaa actgccgacc tgcaatcgat catgaacggc taccacgagt
      901 tggacgggat tccctgcgga gccgatcgcg acctgctcac cggccttctc cggaacgagt
      961 ggggcttcga cggctcggtc gtctctgact acttctcgat acgtcagctc gccagttacc
     1021 accatctgac cgacagtgcg gttgacgcag ctgcaatcgc attgaacgca ggcctcgacg
     1081 tcgaactgcc cagcaccgac tgcttcggcg accccctatt ggaggcaatc cgatcgggct
     1141 cggtcagtga ggagacactg gacaacgccg tgcgcagggt cctgggtgcc aagttcgtgc
     1201 tcggcctgtt cgagaacccc aacgtggacc ccgacaacgc gtcctcggtg gttgacgccc
     1261 cgttccaccg ggatctggcc cgccagatcg cccgcaagac catggtgctc ctcaagaaca
     1321 acggtgtcct cccgctgaag gacgacctgt cgtcgatcgc agtgatcggg cccaacgccg
     1381 acgtcgctcg caacatgttc ggtgattaca cgtatccggc gcacatcgag tccctgatgg
     1441 agatgcggca ccgagagaat gtcttttcca tcccgtttcc cgacgacggg caggtcgacg
     1501 atccaacgcc gaaggggcca accgttgtag acgcacttcg tgaccgattc ggagagaaag
     1561 tgcgttatgc ccctggctgt gacattcagg gcgagtcgag ggacggattc gaagaggccg
     1621 tcgccttggc ggcagagtcg gagttggcga tcctggtgat gggggacaag gcagggctca
     1681 cagaggactg caccaccggc gagggtcgag atcgcctgtc gctcgacctg cctggcgtcc
     1741 aggaagatct ggtcagagcg gtggtcggca ccggaacccc ggtagtcctc gtcctagtcg
     1801 caggacggcc gtgcggaagc gttcttgccc accagagctc cggagccgta atcgcggccg
     1861 ggctgcccgg ccaggaaggc gcagccgcga tcgtggacgt gctcagcggc gacttcaatc
     1921 ctggcggaaa actcgcgatg tcacacccac gaacggtggg tcagattcct gtgttctatg
     1981 gccacaaggt gtcaggcggg cggtcgcact ggaagggcga ctatgtcgat ggatctgccc
     2041 gacctcttta ttcattcgga cacggcctca gttacacggc attcgagctc tcggaaacga
     2101 cagtcgccgg cggacaagcc gggtggaacg acacggtcac tatcacgacg cgtgtttcaa
     2161 acgtggggtc gcgcgatggt gacgaagtcg tgcagttgta tgtgcgtgac cccgaagcca
     2221 gcgtcacccg acccgtcttg gagttgaagg ccttcaagag ggtcgaagtg ccggccggag
     2281 agtccgccct gctgcgattt ggcttgccaa tcggccagat cggcttctac aaccgggacc
     2341 ttgactacgt cgtcgagccc ggtcgggtcg atttgctgat agggacatca tcgtccgacc
     2401 tggtcgaaat cggattcata accgtggtcc ccgacccttc aggagagccg ccccgcaagg
     2461 tcttcgacgg tcgggtcgag gtggaaatga cctaattgtc aggtgaag
//
LOCUS       DBNHXU010000349         4591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_334053, whole genome shotgun sequence.
ACCESSION   DBNHXU010000349 DBNHXU010000000
VERSION     DBNHXU010000349.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4591
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_334053"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="AC161X_08515"
     CDS             <1..411
                     /locus_tag="AC161X_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014618.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nuclease family protein"
                     /protein_id="HIR4989634.1"
                     /translation="YLPIWIGGSEATAIATALEGVNPPRPLTHDLMRSVIDAVGAFAV
                     RVVITEMRDSVFYADLSLDISGREVHVSSRPSDAIALAVRTGTPVFALPGVLEDAGVV
                     FEDEEADEEDEVARFREFLEEVTVEDFLGGEPGD"
     gene            522..980
                     /locus_tag="AC161X_08520"
     CDS             522..980
                     /locus_tag="AC161X_08520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018118949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HIR4989635.1"
                     /translation="MIQGYRAPQVCKLVGITYRQLDYWARTNLIKPSLQQANGSGSQR
                     LYSFSDVVQLKVIKRLLDAGMSLKKIRQAVEILAEQLDSDRPLADVTLLSDGQTIYAA
                     HSEQEVVDVFRRGQGVFGIAIGPVQDEVEGELHRLFPESELAGVVEAGAT"
     gene            1166..1414
                     /locus_tag="AC161X_08525"
     CDS             1166..1414
                     /locus_tag="AC161X_08525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989636.1"
                     /translation="MYIEITTMNQKLVTKKNRKARRLRELYPDVTCKVFYQRDYLNLV
                     VKYGLEEPDHLEDVGAPTRIPGRPEIVRLEDRGERLTG"
     gene            1411..2472
                     /gene="gcvT"
                     /locus_tag="AC161X_08530"
     CDS             1411..2472
                     /gene="gcvT"
                     /locus_tag="AC161X_08530"
                     /EC_number="2.1.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR00528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004047 - aminomethyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system aminomethyltransferase
                     GcvT"
                     /protein_id="HIR4989637.1"
                     /translation="MNRSPLDDLHRRLGARFVDFGGWEMPVQYDSVLAEHRAVRTGAG
                     VFDVSHLGRFELRGPGAKHAISRLLCNDLERIEPGRTQYTMMLNPQGGIIDDLILWWW
                     EPDRLWVMPNAANQERVMAVFSAEPEVTTRSLRDETVLLALQGPSALEIFNSVVGVAP
                     ARFRVAEASWEGGEVDMAGTGYTGERGGEIVCDPVTGARLFEAFLAAGAVPCGLGARD
                     TLRLEAGLPLWGQDLDEATTPYEANLGFAVSLEHDFVGHAALMRQKADGVPRRLTGFV
                     LEERGIPRHGYPARTAHGAGAVTSGNLSPILDKGVGMAYISPPPESGETLLEVEIRDR
                     WVPATIRTPPFHKDPTTNS"
     gene            2484..2987
                     /locus_tag="AC161X_08535"
     CDS             2484..2987
                     /locus_tag="AC161X_08535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012256497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR4989638.1"
                     /translation="MSVRVTGRPEMPEGYGVVDTGPLLPWDTVEERLVEAVNYWLSTA
                     RPDGRPHVVPRWGVWIDEKFFYDGSPTTRHARNLEVNPHCALHLEDGTAVTIVEGVST
                     VPSPIVGELGVRLSDEFKRKYAALGYAPEPTSWSDESAGGLHVLAPTSAIAWSVFPKD
                     LTRFEFD"
     gene            3031..3831
                     /locus_tag="AC161X_08540"
     CDS             3031..3831
                     /locus_tag="AC161X_08540"
                     /inference="COORDINATES: protein motif:HMM:NF023568.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HIR4989639.1"
                     /translation="MSRPEPVEGFGIEQVREWSPGGAPTAVVVLVHGIAEHSGRYERT
                     GTLLAEAGFLVRAFDLFGFGASGGPRGDISDWSIYHDQIERHLGWARGQDVKVVLMGH
                     SMGGTLAAGYVLSGRPAPDLLVLSAPALSGGAAWQRVLAGVLSKVVPSLAIPNSLKGT
                     QLSRDPAVGEAYFSDPLVYPKTTTRLGSLFFTAMTDVTENSHRLDLPTLVLHGGADSI
                     VPPQSTAPLGQLPGVERHLYPGLQHEILNEPEGPEIVGEIASWISAHL"
     gene            complement(3850..>4591)
                     /locus_tag="AC161X_08545"
     CDS             complement(3850..>4591)
                     /locus_tag="AC161X_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020711204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR4989640.1"
                     /translation="CLAGRRHLCHDTKGIGVNLPGAFAEYVAIPKSNVWRHIPGIDFE
                     IAAIFDPFGNAVHTALEFPVLGEDVLITGAGPIGLMASAVVAQAGARNIVITDINPYR
                     LELAKRMGATRAVDVRFEDLQDVQDELGMTEGFDVALEMSGKSAALDDILQNTIHGAN
                     VALLGIPADPYPIDWSIVIFNMLNLKGIYGREMFETWYKMNVLIGTGLDISPVITHRF
                     EVEEFDTAFEVAASHESGKVLINWAGAG"
BASE COUNT          848 a         1365 c         1494 g          884 t
ORIGIN      
        1 taccttccga tatggatcgg cggctccgag gcgacggcca tcgccacggc tctcgagggc
       61 gtgaacccac cccgcccgct gacccacgat ctgatgcgct cggtgatcga cgcagtcggc
      121 gcattcgcag tgcgagtggt gatcaccgag atgcgtgatt cggtcttcta cgccgacctc
      181 tcactcgaca tctcgggtcg tgaggtgcac gtctcatccc gaccctctga tgcgatcgcc
      241 ctcgcggtgc ggactgggac cccggtcttc gcccttcccg gggtcctgga ggacgcaggt
      301 gtcgtcttcg aagatgagga ggcagacgag gaggacgagg tcgcccgttt ccgcgagttc
      361 ctcgaagagg tgacggtgga agacttcctt gggggagagc ccggagactg actgaaaacc
      421 ggtctcgacg agttatccac aggatttcgt tgacattgca tcgggtacat gggtagcttg
      481 ctgacaacgg cggagttaca agcgtgtaac acggaggacc gatgatccag ggctatcgcg
      541 ctccacaggt atgcaagttg gtaggcatca cctatcggca gttggattat tgggcgcgca
      601 ccaacctcat caagccttcc ctgcagcagg cgaacggctc cggatcccaa cggctgtact
      661 cgttttctga cgtggttcag ctcaaggtga tcaagcgcct cctcgacgca gggatgagtc
      721 tgaagaagat ccgccaggcg gttgagatcc tggccgagca gctcgactcg gaccgtcctc
      781 tcgccgacgt gactctcctc agcgatggtc aaaccattta tgcagcacat tccgaacagg
      841 aagttgtgga tgtcttccgc cggggccagg gtgtcttcgg gattgccatt ggcccggtgc
      901 aagacgaagt cgaaggtgag ctgcaccgtc ttttcccgga gagcgagttg gccggggtcg
      961 tcgaggcggg agccacctga tcaccgaact tcaatcggcc gttgagaccg agtttgctca
     1021 caacagtgag cggcagttcg cacttctcct cgatttctac ggagtcccct gggaatacga
     1081 acctcgggct ttcgccctcg cctgggacaa ggagggcact ccgacccagt tcttcacccc
     1141 ggacttctac ctgcccgacg atgacatgta catcgagatc accacgatga accagaagct
     1201 cgtcaccaag aagaaccgca aggcgagacg cctccgcgag ctgtatccgg atgtgacctg
     1261 caaggtcttc tatcagcggg attacctcaa cctggtggtc aagtacgggc tggaggagcc
     1321 ggaccatctc gaagatgtgg gggcgccgac ccggatcccg gggcggcccg agatcgtgcg
     1381 tctcgaagac aggggagagc ggctgaccgg atgaatcggt cccccctcga cgacctccat
     1441 cggaggttag gggcgcgttt cgtcgatttc ggtggatggg agatgcccgt ccaatacgac
     1501 tcggttctcg ctgagcatcg cgccgtgcgc accggtgccg gggtgttcga cgtaagtcac
     1561 ctgggcaggt tcgagttgag gggtcccggg gcgaagcacg ccatcagccg tcttctctgc
     1621 aacgacctcg agcgaatcga gccgggccgg actcagtaca cgatgatgct caacccccag
     1681 ggtgggatca tcgatgacct gattctctgg tggtgggagc ccgatcggct ctgggtcatg
     1741 ccgaatgcgg ccaaccagga gagggtcatg gcggtattca gtgccgagcc cgaggtgacg
     1801 acacgaagcc tccgcgatga aacggtcctg cttgcactcc agggaccgtc cgctctcgag
     1861 atattcaact cggtggtggg agtggcacca gccaggttca gggtggcgga ggcgagttgg
     1921 gaaggcggcg aggtggacat ggctggcacc ggctacaccg gagagcgtgg cggtgagatc
     1981 gtctgtgatc ctgtgaccgg agccaggctc tttgaagcgt ttctggcggc cggagctgtc
     2041 ccatgcggac tgggggcgag ggacacactg cgactcgagg ccggtctgcc gctctggggt
     2101 caggacctag acgaggccac cacgccatat gaggccaacc tcgggtttgc agtgagtctg
     2161 gaacatgact ttgtcggcca cgccgcattg atgaggcaga aggcggatgg agtgcctcgt
     2221 cgactgaccg gtttcgttct cgaggagcgg ggtatccctc gccacggcta cccggcgcgg
     2281 acggctcacg gcgccggtgc ggtcacttcg gggaatctca gcccgatcct ggacaaaggg
     2341 gtgggcatgg catacatctc cccgccgccc gaatcaggag aaacgttgct cgaggtcgag
     2401 atccgcgatc gctgggtgcc ggcaacgatc aggactccgc ctttccacaa ggacccaaca
     2461 accaactctt aggatctcgc cacatgtctg tgagagtgac cgggcgaccc gagatgcccg
     2521 aaggctatgg cgttgtcgat accggtccac tcctgccatg ggataccgtt gaggagcgtc
     2581 tcgtggaggc tgtgaactac tggctgagca ctgcacgccc cgatgggcgc cctcacgtgg
     2641 ttccgcggtg gggcgtttgg atcgacgaga agttcttcta tgacggctcc ccaacgaccc
     2701 gtcacgcccg aaacctcgag gtgaacccgc attgcgcatt gcatctcgaa gatggcaccg
     2761 cggtgaccat tgtcgagggc gtctccaccg tgccgtcacc gattgttggc gagctcggtg
     2821 tcaggctgtc cgacgagttc aagcgcaagt acgcggcgtt gggttatgcc ccggagccga
     2881 catcatggtc cgacgagtcg gccgggggat tgcatgtcct ggccccgacc tcggcgatcg
     2941 catggtcggt gtttcccaag gatctgacgc gtttcgaatt cgactgacca cactgaactg
     3001 agacaaggcg aggagagtca tttgaacagc gtgtcacgac cggagcccgt cgaggggttc
     3061 gggatcgaac aggtgaggga gtggtcaccg ggtggcgccc cgacggccgt ggtagtcctg
     3121 gtgcacggga tcgccgagca ctccggtcgc tacgagagaa ccggaacatt gctcgccgag
     3181 gcggggtttc tggttcgggc cttcgatctg ttcggattcg gcgcttcggg tgggccgcgt
     3241 ggcgacatct ccgattggag catctatcac gaccagatcg agcgtcacct cggatgggcg
     3301 cgtggtcaag acgtcaaagt tgttctcatg gggcattcca tggggggcac cctcgccgcc
     3361 ggttacgttc tctcggggag gccggcgcct gacttgttgg tgttgagcgc tccggctctc
     3421 agcggcgggg cagcgtggca acgggttctt gcaggcgtgc tgagcaaggt cgtgccctct
     3481 ttggcgattc cgaactcgct gaagggaacg caactctcgc gtgacccggc ggtaggcgag
     3541 gcctacttca gcgacccgct ggtgtacccc aagaccacga caaggctcgg ttcactcttc
     3601 ttcaccgcaa tgaccgacgt gaccgagaat tcccaccggc tcgacctccc cactcttgtc
     3661 ctgcatggag gcgccgacag catcgtccca ccccaatcca ctgcaccgct gggccagttg
     3721 cccggagtgg aacgtcacct ctacccgggg ctgcaacacg agatcctcaa tgagcccgag
     3781 gggccggaga tagtcggcga gattgctagc tggatctcag cccacttgtg aggatggctt
     3841 cgagcgccgt cagcccgccc cggcccagtt gatcagaacc ttccccgact cgtgggaagc
     3901 ggccacctcg aaagcggtgt cgaattcctc cacctcgaag cggtgggtga tgaccgggga
     3961 gatatcgagg ccggtgccga tcagcacatt catcttgtac caggtctcga acatctcacg
     4021 cccatagatg cccttgaggt tgagcatgtt gaagatgacg atgctccaat cgatggggta
     4081 agggtccgcg gggattccca gcaaggccac attggccccg tggatggtgt tctggaggat
     4141 gtcatcgagg gcggccgact tgccggacat ctccagcgcg acatcgaagc cttcggtcat
     4201 cccgagctcg tcctggacat cctggagatc ttcgaagcgc acgtctaccg cgcgggttgc
     4261 tcccatccgt ttcgcgagct cgaggcgata tgggttgatg tcggtgatga cgatgttgcg
     4321 tgcgccggcc tgagccacga cggcggatgc catcaaaccg atggggccgg caccggtgat
     4381 caggacgtct tcgccaagca ccgggaactc gagggcggtg tgcaccgcgt tcccgaaggg
     4441 gtcgaagatg gctgcgatct cgaaatcgat gcctggtatg tgcctccaca cgttgctctt
     4501 ggggatggcg acgtactcgg cgaaggcacc ggggaggttg acccctatcc ccttggtgtc
     4561 gtgacatagg tgccgccggc cggcgaggca a
//
LOCUS       DBNHXU010000350         1591 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3704198, whole genome shotgun sequence.
ACCESSION   DBNHXU010000350 DBNHXU010000000
VERSION     DBNHXU010000350.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1591)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1591)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1591
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3704198"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..1285
                     /locus_tag="AC161X_08550"
     CDS             179..1285
                     /locus_tag="AC161X_08550"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010360994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR4989641.1"
                     /translation="MTSDSLRRFDRAGRIGRLRQLMSEAGIDVVMLSVGSDLPYFTGY
                     EAMASERLTMLIVRQTGDPVLLVPALEAARVPPGPFDLLSWGDTDDPIEMVTRAVGHP
                     GKVAVGDHTWSTFLLALQSAIPDAEWQVASEMTRQLRIRKDAAEIEMMRAAAEAADRA
                     LARAASEVRFAGRTERQVARDFALLTVEEGHDVAAFTIIGSGPNSASPHHEPGLRVMQ
                     EGDVVVCDFGGRIGGYYSDVTRTFSVGEVGKEETEVHALVEEANRVGRSVVAPGVTCQ
                     EVDRWARSVIAGAGYGRFFIHRTGHGIGLEVHEHPYMVEGNDMLMQPGMAFSVEPGIY
                     LPGRFGVRIEDIVVCTETGADVLNKADRGLVIVA"
     gene            complement(1282..>1591)
                     /locus_tag="AC161X_08555"
     CDS             complement(1282..>1591)
                     /locus_tag="AC161X_08555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989642.1"
                     /translation="TCSLIVDTFSGRTHLIQEGFFQLLPYQGNEQAQFTSGSTTFTLA
                     LDGTTVSTTQLPISSSSTQATRLWNTTLVFAGGNHTLVGEWRWNGTLVQKTTATIRVD
                     "
BASE COUNT          291 a          447 c          532 g          321 t
ORIGIN      
        1 cgcgaccgga tcggctgcgg ccggatacac gccttgagcc atgacgaagg cgatgatgag
       61 ggtgatgacg attctcaggc gcattgccgt cacccaaaga gaccgtagcc cgagaacccg
      121 attacgcagt caatcgaggg gcgatggtta acgtcatctc gttttggtgg agactcgtgt
      181 gacctctgat tcgctacgtc gcttcgaccg cgccggtcgc atagggcggc ttcgccaact
      241 gatgtccgag gctggcatcg atgtcgtgat gctctccgtg ggatcagatc tcccatactt
      301 cacaggttat gaggcgatgg cctccgagcg gctgacgatg ttgatcgttc gccagaccgg
      361 tgaccctgtg ctcctcgtcc cggccctgga ggcagcccgc gtccccccag gaccgttcga
      421 tttgttgtca tggggtgaca ccgacgatcc aattgagatg gtgactcgtg cggtcgggca
      481 tcctggcaaa gtggcggttg gagatcacac gtggtccaca ttcctccttg ccctccaaag
      541 cgcgataccc gatgctgagt ggcaagtcgc ctccgagatg acgaggcaac ttcggatccg
      601 aaaggacgca gcggagatcg agatgatgag agccgccgcc gaggcggcgg accgggctct
      661 cgccagggcg gcctcggaag tccggttcgc cggcaggacc gagcgccagg tggctcgcga
      721 cttcgccctc ttgaccgtgg aagagggaca cgatgtcgcc gcgttcacga tcatcggatc
      781 gggcccgaac tcggcctctc ctcatcatga gcctggcttg cgggtcatgc aggaaggtga
      841 tgtggtggtc tgtgactttg gtggtcgaat tggtggctac tactcagacg tcacccggac
      901 cttttcggtc ggtgaagttg gcaaggagga gaccgaggtc cacgcactgg tcgaggaggc
      961 gaaccgtgtt ggccgctccg tagtcgcccc aggagtcacc tgtcaggagg tcgaccgctg
     1021 ggcgcgttcg gtaatcgcgg gagcgggcta tggccgtttc ttcatccatc ggaccggcca
     1081 tggcataggt ctcgaggttc acgaacaccc ttacatggtc gaaggcaacg acatgctgat
     1141 gcaaccgggc atggctttct cagtcgagcc ggggatctat ctgccagggc ggttcggggt
     1201 caggatcgag gacatcgtgg tatgtaccga gaccggcgcg gacgtcctga acaaggctga
     1261 ccgcggcctc gtcattgtcg cctagtcgac ccggatggtg gcggtggtct tctgaaccag
     1321 cgtgccgttc cagcgccact cgccaaccag agtgtggttt ccgccggcga aaacgagggt
     1381 ggtgttccaa agccgggtgg cttgggtcga tgacgacgag ataggcagtt gggttgtgga
     1441 gaccgttgtc ccgtcgaggg ccaacgtgaa ggtggtgctc cccgacgtga attgggcctg
     1501 ttcatttcct tgatagggga gcagttgaaa gaacccctcc tggatgaggt gggtgcgccc
     1561 actgaaggtg tcgacgatca gagaacacgt c
//
LOCUS       DBNHXU010000351         6620 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1721225, whole genome shotgun sequence.
ACCESSION   DBNHXU010000351 DBNHXU010000000
VERSION     DBNHXU010000351.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6620)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6620)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6620
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1721225"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="AC161X_08560"
     CDS             <1..545
                     /locus_tag="AC161X_08560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4989643.1"
                     /translation="TTGLTIVAVAMSILATITVDTGYGRVAIALAILGIGMGTAMAPA
                     TESIMGSLPLAKAGVGSAMNDTTRQIGGALGVAILGSILASSYGNAMAPVVANLPGEA
                     AQIAGDSIGGAVAVASQIGDAGAQLVDAASAAFIGGMEIAVWVAAGVVLLGAVITYLF
                     LPARALEVTEMSPVPADDIH"
     gene            532..1119
                     /locus_tag="AC161X_08565"
     CDS             532..1119
                     /locus_tag="AC161X_08565"
                     /inference="COORDINATES: protein motif:HMM:NF028169.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HIR4989644.1"
                     /translation="MTSTDRKPGRPRNASVDAAIIQAALEMLDEVGLQAVSMDGVATR
                     AGVGKATIYRRWDSREDLLIDAVAGLVASVEVPETDDLRADLVTLLVRMRTFMSEMKG
                     GSIFPWLIGEVAAGTEVGKHYADAVILPRRAMIAQVIRRAIENGSLRRDLDVQLAVDM
                     ISGPTLLRKMMTPIRGVDERWEEKLVDALLDGWRS"
     gene            complement(1120..2370)
                     /gene="hisD"
                     /locus_tag="AC161X_08570"
     CDS             complement(1120..2370)
                     /gene="hisD"
                     /locus_tag="AC161X_08570"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: protein motif:HMM:NF013013.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HIR4989645.1"
                     /translation="MNYLRRANPQTVTASRRPVAMETLTEAALIVEDVRLRGDEALRR
                     HAERLGDIAPGEPMVLERDQLEKALENVGDEARVVLESVAQRIRAFAESQRQAVLDLD
                     VEVTGGRAGHNWIPVASVGAYAPGGRYPLPSSVLMTVVPARVAGVDSVWLASPRPTPV
                     TMAAAAVAGVDGLLAVGGAQAIAALAFGTLTPQCDLVVGPGNRWVTAAKKYLFGEIGI
                     DGLAGPSEILVIADDSAIAELVAADLLAQAEHDDDAVPMLVTTSQNLANAVDEAIGRQ
                     LSDLPTSETASRALRNGFCLIVGSLEEASSMSNRLAPEHLALHVDDPGSLLPQLRSYG
                     SVFVGSRTAEAFADYGVGPNHVLPTGGGARYQSGLSVLTYLRSPTWTALDDPGPLVED
                     TVLLARLEGLEAHARAAQLRAAGS"
     gene            complement(2367..3623)
                     /gene="hisE"
                     /locus_tag="AC161X_08575"
     CDS             complement(2367..3623)
                     /gene="hisE"
                     /locus_tag="AC161X_08575"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013168.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HIR4989646.1"
                     /translation="MIIPSIDISRGQAVQLVEGEAVAIEAGDPFPLMERFSVAGEVAV
                     IDIDAARGQGDNRQLISELCRRGRVRLGGGIRDLDTALWWLDQGVSRIIIGTAATPEF
                     LLSLPRERVIVALDSRDSQVLSHGWRQSTGETLLDRVSELNQSCDGFLVTFVEREGRL
                     GGTDLDRAKQVIDAAGDARVTVAGGISTVEEIAELDRLGADAQVGMALYTGDITLADA
                     LAAPLVSDRADGLWPTVVVDEAGVALGLAWSSHESLRQAIETKRGAYESRSRGLWIKG
                     ETSGAVQELVGVAADCDRDTLRFTVRQHGAGFCHTGERTCWGADQGLGRLQRRILATG
                     DDPVSNTRKLLGDPALLAAKIREEAEELAETEDRAQVVTEAADLLYFLMVKTAATGVG
                     LDEIEAELDRRAKRVTRRPMVAKDAQ"
     gene            complement(3620..4375)
                     /gene="hisF"
                     /locus_tag="AC161X_08580"
     CDS             complement(3620..4375)
                     /gene="hisF"
                     /locus_tag="AC161X_08580"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005526281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HIR4989647.1"
                     /translation="MMLAARVIPCLDIKDGRVVKGVRFQELRDAGDPVENAATYEAQG
                     ADEIVLLDVSATPEGRGHAVDTVRAVRSVLSIPLTVGGGVRRESDAGRLLDAGADKVG
                     INTAAVARPDLIADISSRFGSQCTVLAVDAARTETGWEVVVRSGAERTGIDVVEWCRK
                     ATALGAGEILLTSWDRDGTRDGYDVDLVAAVAESVSVPVIASGGAARSEHLVAALQAG
                     ASAALVASILHDGDTTVSDLKAGLTRAGITVRR"
     gene            complement(4372..4959)
                     /gene="hisH"
                     /locus_tag="AC161X_08585"
     CDS             complement(4372..4959)
                     /gene="hisH"
                     /locus_tag="AC161X_08585"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisH"
                     /protein_id="HIR4989648.1"
                     /translation="MVIVPTGTANTASVIAAFQRLDVEPVLAADSQMVDEADRVVLPG
                     VGAFGPAIDRIDQLGMREAMTSRIENDQPTLAICLGMQLLCGASDESPDSVGLGVVQQ
                     SVTRFTGDVKVPQLGWNRVEPDRSNWLEPGWAYFANSYMVPRLPEGWSGATTDYGGSF
                     VSAMARGSVLACQFHPELSGAWGSNLLRRWVWRDA"
     gene            complement(4965..5549)
                     /gene="hisB"
                     /locus_tag="AC161X_08590"
     CDS             complement(4965..5549)
                     /gene="hisB"
                     /locus_tag="AC161X_08590"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015740357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - L-histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HIR4989649.1"
                     /translation="MTRKSEITRATSETNVECRLDLGGVGTSDVKTGLGFLDHMLTAL
                     ARHSRIDLTLLATGDTEVDDHHTVEDCAIVLGRALNEALGDRSGITRFGYSYAPLDES
                     LTRAVVDLSGRPWPEINLSLSRDTINDVASENLTHFFRTLAMEARMALHVDVIRGDND
                     HHKAESAFKAVALALREAVAVSGDQVPSTKGSLT"
     gene            complement(5546..>6620)
                     /locus_tag="AC161X_08595"
     CDS             complement(5546..>6620)
                     /locus_tag="AC161X_08595"
                     /inference="COORDINATES: protein motif:HMM:NF024811.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR4989650.1"
                     /translation="GLAGLRVGYLLGPPELVAEIGAHGNPYPVASISAALATTRLGRP
                     ASELNDFVEEIKRERVELTSTLGELGTKPFPSEANFVLTQCRDPSWLISAAAALGVGL
                     RHFPDRPDLGQMVRITLPGNRPDFQRLVETLQTALAPEAIVFDLDGVLADVSRSQSTA
                     IIETGRSFGIEIDVTDIERAKANGNSNDDWLLTQGLCREKGTDVALDVVTDRYESLYQ
                     GSNSKPGLKTHESPIVDGTTWARWASSRPLAVVTGRPRADAEEFLDRFDLARQVSALV
                     TRDDAPLKPDPAPVSLALQLLGVRRAWMLGDTPDDLTAARAAGVIPIGVIAPGDDPKR
                     SRLSLRNAARILENTNQLEALLP"
BASE COUNT         1132 a         2165 c         2177 g         1146 t
ORIGIN      
        1 tcaccaccgg tctcaccatc gtcgcggtgg caatgtcgat cctggccacg atcacggtcg
       61 ataccgggta cggaagagtg gcgatcgccc tggcgatcct tggcatcggc atggggaccg
      121 ccatggcccc ggccaccgag tcgatcatgg gctccctgcc gctcgccaag gccggtgtgg
      181 gctcggcgat gaacgacacc actcgtcaga tcggcggggc gctaggagtc gccatcctgg
      241 gcagcatcct ggcttcgtca tacggcaacg ccatggcacc ggtggtggcc aacctcccag
      301 gtgaggcggc acagatcgcg ggtgactcga tcggcggggc cgtggcggtg gcttcccaga
      361 tcggcgacgc cggggcgcaa ctggtcgatg ccgcttccgc agcattcatc ggtgggatgg
      421 agatcgcggt ctgggtcgcc gccggagtgg tgctgctcgg agcggttatc acttacctgt
      481 tcctgcccgc ccgcgcccta gaagtgaccg aaatgagccc agttccagcc gatgacatcc
      541 actgatcgca aaccggggcg gccccggaac gcatcggtag acgccgccat catccaggct
      601 gccctggaga tgctggacga agtcggcctc caagccgtca gcatggacgg tgtggctacc
      661 cgagccgggg tgggcaaggc gacgatctac cggcgttggg attcgcgaga ggacctgctc
      721 atcgacgcgg tagccggtct ggtggcttca gtggaagttc cggagaccga cgacctaagg
      781 gccgatctcg tcacgctcct ggtgcgtatg cgcaccttca tgtccgagat gaagggcgga
      841 agcatctttc cctggcttat cggcgaggtg gctgccggca ccgaggtggg aaagcactac
      901 gccgatgcgg tgatcctgcc caggcgggcg atgatcgccc aggtgattag gcgggcgatc
      961 gaaaacggct cgctgcgtcg ggatctcgat gtgcaacttg cggtggacat gatcagcggg
     1021 ccgacacttc ttcggaagat gatgaccccg atacgaggtg tcgacgagag atgggaagag
     1081 aagctggtcg acgccttgct cgatgggtgg cgcagctgat caacttccgg cggccctgag
     1141 ctgagcggct cgggcatggg cctccaggcc ctcgagacgg gcaaggagga ctgtgtcctc
     1201 gaccaatggc cctggatcat cgagtgcggt ccatgtgggt gaccggagat aggtgagcac
     1261 tgacagaccc gactgatatc gggcgccacc acccgtcggc aatacgtggt tggggccgac
     1321 gccatagtcg gcgaaggctt cggcagtccg gctgccgacg aacacagagc catacgagcg
     1381 gagctggggg agaagtgatc cggggtcgtc gacatgtagt gccagatgtt cgggcgccag
     1441 gcgattggac atcgagctcg cctcttcgag cgatccgaca atcaggcaaa acccgttgcg
     1501 aagggcgcgc gaagccgtct ccgaggtggg aaggtcggac agctgccgcc cgatcgcctc
     1561 gtcgactgcg ttagccaggt tctggctggt ggtgaccagc atcggcaccg cgtcatcgtc
     1621 atgctcggct tgggccagga gatcggctgc gaccagttcc gcgatggctg agtcgtcggc
     1681 gatgacgagg atctcgctag gtcccgccag cccgtcgatg ccgatctccc cgaagaggta
     1741 tttctttgcc gcagtcaccc accgatttcc cggcccgacc accagatcgc attgcggggt
     1801 gagagtgccg aaggcgaggg cggcgatggc ctgtgccccg cccaccgcca gaaggccatc
     1861 gactccggcg actgcggctg ctgccatagt gaccggcgtc ggccggggag aggcgagcca
     1921 gaccgaatcc acaccggcta cccgggcggg gaccaccgtc atcaacaccg acgagggaag
     1981 cgggtatcgg cctccggggg cgtaggcccc aaccgatgcc acagggatcc agttgtgccc
     2041 ggctctccca ccggtcacct cgacgtccaa gtcgagcacc gcctgtctct gggactcagc
     2101 gaaggctcgg attcgttggg caacgctttc caagaccacc ctggcctcgt cacccacgtt
     2161 ctccagggcc ttctcgagct ggtctcgttc gagaaccatc ggctctccag gcgcgatgtc
     2221 accgagtcgc tctgcgtgcc ggcgcagcgc ctcgtcaccg cgaagccgga cgtcttccac
     2281 gatgagtgcc gcttctgtga gggtctccat cgccaccggt cgccgggagg cagtgacggt
     2341 ttgagggttg gctcggcgga ggtagttcat tgggcgtcct tggccaccat cgggcggcgg
     2401 gtgacccgtt tcgcccgccg gtcgagctcg gcctcgatct cgtccagacc cacaccggtg
     2461 gccgcggtct tgaccatgag gaagtagaga aggtccgccg cctccgtgac cacctgggcg
     2521 cgatcttctg tctcggccag ctcctccgcc tcttcgcgga tcttcgctgc gagcagggcc
     2581 gggtccccga gcagctttcg ggtgttcgag acgggatcgt cgccggtggc cagaatgcgc
     2641 ctctggagac ggccgagacc ttggtcggcg ccccagcagg ttcgctcgcc cgtgtggcag
     2701 aagccggcgc cgtgctgacg gacggtgaat cgaagcgtgt cacggtcgca gtcggctgcg
     2761 acaccgacaa gctcctgcac tgctcccgag gtctcccctt tgatccaaag gcctcgcgag
     2821 cggctctcat aggctccgcg cttggtctcg atggcttgcc taaggctctc atggctggac
     2881 caggccagtc cgagggcgac tccggcctcg tccaccacca ctgtcggcca caagccatca
     2941 gcccggtccg aaaccagggg agcggcgagc gcatccgcca gcgtgatatc cccggtgtag
     3001 agcgccatgc cgacctgggc gtcggcgccg agtcgatcga gctcggcaat ctcctcgacc
     3061 gtggatatcc ccccagcgac agtcaccctt gcatcacccg cggcgtcgat gacctgcttg
     3121 gcgcggtcga ggtcggtgcc gccgaggcgg ccctcccgtt cgacgaaagt gaccagaaat
     3181 ccgtcacaag actggttcaa ctctgacaca cggtcgagca gcgtttcccc cgtcgactgg
     3241 cgccaaccgt ggctgaggac ctggctatct ctcgagtcga gggcaacgat cacccgctcc
     3301 cgggggagcg aaaggagaaa ctcgggcgtg gccgcggtcc caatgatgat cctgctcaca
     3361 ccctggtcga gccaccacag ggcggtgtcg aggtcccgga ttccgccacc gagccggacc
     3421 cgtccccgac ggcacaattc cgatatcagc tgccgattgt ctccctggcc ccgggcggca
     3481 tcgatgtcga tcaccgcgac ttcgcctgcc accgagaagc gctccatcag gggaaacggg
     3541 tcgcccgcct cgatagcaac agcttcgcct tcgacgagct ggacggcctg gccccgcgag
     3601 atgtcgatgg aggggatgat catcgccgca ccgtgatccc ggcccgggtc agccctgcct
     3661 tgaggtcgct gaccgtggtg tcgccatcgt ggagaatcga ggccaccagg gcggccgagg
     3721 ccccggcctg tagggcggct accagatgct cggatcgcgc cgcacccccc gaggcgatca
     3781 ccggcaccga caccgattcc gccaccgcgg cgaccagatc cacgtcgtag ccatctcggg
     3841 ttccgtcgcg gtcccagctg gtgagcagga tttccccagc tcctagtgca gtcgccttgc
     3901 ggcaccattc gacgacgtcg attccggtcc gctcggcacc gctccgcacg accacctccc
     3961 aaccggtctc ggtacgagcg gcatcgaccg cgagcaccgt gcactgcgac ccgaaacgcg
     4021 aggagatatc ggctatcagg tcgggccggg ccacggccgc cgtgttgatg ccgaccttgt
     4081 ccgctcctgc gtcgagcaat cgcccggcgt cggactcgcg gcggacacca cctcccacgg
     4141 tgagtgggat cgaaagaacc gaacgcaccg ctcgcactgt gtcgaccgcg tgtccgcgac
     4201 cctccggcgt ggcggagaca tcgaggagga cgatctcgtc cgccccttgc gcctcatagg
     4261 tcgcggcgtt ctccacgggg tcgcccgcat ctcggagctc ttgaaagcga acccctttga
     4321 ccacccgccc gtccttgatg tcgagacacg ggatcaccct ggccgccagc atcaggcgtc
     4381 cctccagacc cagcgccgca ggaggttcga gccccaggct cccgagagct cgggatggaa
     4441 ctggcaggcc agcaccgaac ctcgtgccat tgcggacaca aaactccctc cataatcggt
     4501 ggtggccccg gaccaccctt ccgggagccg cgggaccatg taggagttgg cgaagtaggc
     4561 ccaacccggt tccagccagt tcgaacgatc cggttccacc ctgttccaac cgagttgggg
     4621 aaccttgaca tcgccggtga accgggtcac cgactgttgc acgacgccca gacccaccga
     4681 atcggggctc tcatcgctag ccccacatag gagttgcatc ccaaggcaga tcgcaagggt
     4741 cggctggtcg ttttcgatgc gcgacgtcat ggcctccctc attccgagct ggtcgatgcg
     4801 gtcgatggcg gggccaaagg caccaacgcc ggggaggacg actcgatccg cttcatcgac
     4861 catctgtgag tcggcggcga gcaccggttc gacgtcgagt ctttggaaag ccgcgatcac
     4921 agaagccgta ttcgccgtgc cggtaggcac gatcaccacc tgtctcaagt caaagacccc
     4981 ttggtgctcg ggacctggtc accggagaca gccaccgcct cgcgcagggc gagggccact
     5041 gccttgaagg cggattccgc tttgtggtgg tcgttgtcac cgcgaatcac gtcgacatga
     5101 agcgccatcc gggcctccat ggccagcgtt cggaagaagt gggtgaggtt ctcggaggcg
     5161 acgtcgttga tggtgtcgcg ggacaatgac agattgatct ccggccatgg tcgtcccgac
     5221 aggtcgacca cggccctcgt cagcgactcg tcgagagggg cgtacgagta accgaatcgg
     5281 gtgatccccg agcggtcgcc gagagcctcg ttgagtgctc gcccgagcac gatggcgcaa
     5341 tcctcgaccg tgtggtggtc gtcgacttca gtgtcaccgg ttgcgagcag ggtcaggtcg
     5401 atgcggctgt gcctggccag cgcggtgagc atgtggtcca ggaagccgag ccccgtcttg
     5461 acgtctgagg tgccgactcc gcccaggtcc aggcggcact cgacattggt ttccgacgtc
     5521 gcccgggtga tctctgattt tcgtgtcatg gcagcagtgc ctccagttgg ttggtgtttt
     5581 cgaggatgcg ggccgcattc ctcaggctga gacgggaccg ttttggatcg tcgccggggg
     5641 cgatgacgcc gatcggaatc actccggccg ctcgggcggc agtgaggtca tcgggcgtgt
     5701 cgccgagcat ccaggctcgc cgaaccccga gcagttgcag ggccaagctc accggcgccg
     5761 gatcgggttt tagaggtgca tcgtcccggg tcaccaacgc ggacacttgt cgcgcgagat
     5821 cgaagcgatc gaggaactcc tcggcgtcgg cacggggccg gccggtgacg accgccaggg
     5881 ggcgggagga ggcccatcgc gcccaggtag tgccatccac aatcggcgac tcgtgggtct
     5941 tgagccctgg tttgctgttt gagccctgat acaggctctc gtagcggtcg gtcaccacgt
     6001 ccagggcgac atcggtcccc ttctcccggc agaggccttg ggtgagaagc caatcgtcgt
     6061 tgctgttgcc gttggccttg gcacgctcga tatcggtgac atcgatctcg attccgaagc
     6121 tgcgcccggt ctcgatgatg gctgtgctct gtgaccggct gacatcggcc aaaaccccat
     6181 ccaagtcgaa gacaatggcc tcgggcgcaa gcgcggtctg cagcgtttcg accaggcgtt
     6241 ggaagtctgg ccggttcccg gggagtgtga ttcgcaccat ttggcccagg tcaggtcgat
     6301 ccgggaagtg acgaagtcca acccccaatg cggcggcagc cgagatcaac cacgatgggt
     6361 cccggcactg ggtgagcacg aagttggcct ccgagggaaa cggcttcgtg cccagctcgc
     6421 cgagcgtgga ggtgagctcg acccgctctc gcttgatttc ctccacgaag tcgttgagct
     6481 cggaagccgg acgacccaag cgcgtcgttg ccagtgcggc ggagatggag gcgacaggat
     6541 atggattccc atgggcgccg atctcggcaa ccaattcggg tggcccgagt aggtagccaa
     6601 ccctgagccc ggccagcccg
//
LOCUS       DBNHXU010000352        16509 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4301882, whole genome shotgun sequence.
ACCESSION   DBNHXU010000352 DBNHXU010000000
VERSION     DBNHXU010000352.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 16509)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16509)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16509
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4301882"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..1047)
                     /locus_tag="AC161X_08600"
     CDS             complement(13..1047)
                     /locus_tag="AC161X_08600"
                     /inference="COORDINATES: protein motif:HMM:NF022702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HIR4989651.1"
                     /translation="MRSTFSKFVVSLLILAACSPAEAIDSTTTSTSTTTTLATTTSSV
                     GTTTTEATTLSPINGLPVADPALLNRRLLAVKIDNHPNARPQSGINHADAVFEIRVEG
                     ISRFLTMWMQSDAEFLGPMRSGRPTDSTLLTALNKPTFAISGAQGWVQEMIRSKDINL
                     LTETTPAAFRVPFRRAPHNLYTTTAGLRADADARDYEDLPPTGPIWEFGPMPTDAAAA
                     SAVHIDYRGTDVDWTWDPTTATWLRSADGRESNWRDEDDTEGRIGFPVLIGLESEQYS
                     NNGLPSSHTTGSGQAWVFADGKVIEGTWERATEQDWYTLTDADGNNIAVPPGQVWISL
                     VPDIGGLTYE"
     gene            complement(1075..1476)
                     /locus_tag="AC161X_08605"
     CDS             complement(1075..1476)
                     /locus_tag="AC161X_08605"
                     /inference="COORDINATES: protein motif:HMM:NF040320.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR4989652.1"
                     /translation="MTEFIVQMENRPGRLASLTEALAAFGVNIEALTAYGTNGEGTVR
                     LIVDDAATTRRVLSEAALQSEERMVLTAQLPHRPGELARLTRVIADAGINIDALYVLR
                     SSAEGIELAVSIDEPESSMPQLPVTGGVLTV"
     gene            complement(1486..2454)
                     /locus_tag="AC161X_08610"
     CDS             complement(1486..2454)
                     /locus_tag="AC161X_08610"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051604 - protein maturation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S26 family signal peptidase"
                     /protein_id="HIR4989653.1"
                     /translation="MSIRRFVVVEESMLPGLAPGDEFVATDARLPASGDVVVFPHPSR
                     DNFWLVKRMAAGPGEEAGGHRLGEDEAWVISDNLDATRADSRTLGPIPFASLWVRVTS
                     LDAGTFGDAAILLADEEPALGLIVDEWGLPPFWQRQPGFSTLVLLILEQQVSLESGAA
                     MFRRLLDLVDVVTAKTVLEAGDTGLRSIGVTRQKVGYLLGLAEAVSGGELDLDALPQT
                     TEPDARARLIALKGIGLWTADAYLLSALRLPDVFPVGDRALQVGTMEALGLDAVPGPR
                     ELETISAPWRPVRAVAARLIWHRYLSTRGRVEPPDPVAGHVVNDPA"
     gene            2506..2913
                     /gene="sodN"
                     /locus_tag="AC161X_08615"
     CDS             2506..2913
                     /gene="sodN"
                     /locus_tag="AC161X_08615"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011892033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0019430 - removal of superoxide radicals
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase, Ni"
                     /protein_id="HIR4989654.1"
                     /translation="MRQLTVAHAHCDLYCGVYDPAQAKIEAMSVLKIAEKYASSDDEV
                     FKARAISLKEERAEEVKHHLMVLWADFFTAEHRTEFPNLDDLFWRAIHQAGDAKKSVD
                     PEDGRKLIALIDEISDVFWQTEKAKGMGVYPPA"
     gene            complement(2915..3121)
                     /locus_tag="AC161X_08620"
     CDS             complement(2915..3121)
                     /locus_tag="AC161X_08620"
                     /inference="COORDINATES: protein motif:HMM:NF021193.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PGPGW domain-containing protein"
                     /protein_id="HIR4989655.1"
                     /translation="MTRIARVVGGFILLIAGIAMLVLPGPGVLTILGGLALIGQEFPW
                     AQRMLDRIKAAMTRLWRSLTQPSA"
     gene            complement(3205..4842)
                     /gene="metG"
                     /locus_tag="AC161X_08625"
     CDS             complement(3205..4842)
                     /gene="metG"
                     /locus_tag="AC161X_08625"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013218700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HIR4989656.1"
                     /translation="MTRRILVAVAWPYASGSLHLGHLGGAYLPSDIFARYHRVAGDDV
                     LMVSGSDAHGTPITVRADEEGITPREVVDKYHAEILGYWEALGISFDLFTTTMTENHH
                     AVTQDVFLKLLEKGHLVKGTSSQFFDPEVGRFLPDRYVEGTCPHCGYTEARGDQCENC
                     GRTLDATDLINPRSRLSGATPVLRETEHFFFRLPEFAEPLKAWLESKTTWRRHVKNWA
                     LGMIEEGMPDRAITRDIEWGVEVPVDDLGPGKRIYVWFDAVIGYLSAAKEWAQNSGDA
                     EAWRNWWEDPEAESYYFVGKDNIPFHTVIWPAMLLGYGGLNLPTDVPANQYITFKGAK
                     ASKSKGVGRSIGWYAERLEPDALRYAIASVLPEQNDTDLSDEEIIRRINDELVATWGN
                     LVNRVLSLTANNFDRKVPEPDELTGEDQALLADVDQALEAVAGHIAAVELRAGLRAAM
                     EAATEVNVYLNANAPWKLVKENPKRAETILWTAIQAISGIRVALSPYLPFTTPKVGEM
                     LGLPGAVASWKRPEVSAGTALGEIQPLFAKLEDDALD"
     gene            complement(4873..5559)
                     /locus_tag="AC161X_08630"
     CDS             complement(4873..5559)
                     /locus_tag="AC161X_08630"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR4989657.1"
                     /translation="MAIPNTFFTMGLVELPVSLSGLMVALIPAATVGAAHFLVEGERF
                     NPKSTPGLIVSLIGTGILVGVGGGSLEGVGNLGLGVTLSLIAVLFAGIGGALTRRFAL
                     EVGGDGLVIPQFAFATAISLLATPFLDATPLDSLESSDWVLLILLGSFATAVPFTAFL
                     IAAQVNPASRLAVAGYLVPILAVTLAVIFLGETLTLAVLIGAVLIIGGVVLTERSSRH
                     VPVPGIDTAG"
     gene            complement(5575..5781)
                     /locus_tag="AC161X_08635"
     CDS             complement(5575..5781)
                     /locus_tag="AC161X_08635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989658.1"
                     /translation="MNPWTPIVVSILGWGSAAVLTRAVLNNGVSAFTVVPFRFGVALI
                     VILLVSIYWVRFRELHRGHWVRGF"
     gene            5855..6937
                     /locus_tag="AC161X_08640"
     CDS             5855..6937
                     /locus_tag="AC161X_08640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989659.1"
                     /translation="MIKRSLFLVAILAMVVTACGGTVETSTTAAADSTTTAPPAEAVL
                     LSYSLTAGDEFKYEVGLDQHIELTTSGDASAMGEEDVPEAASVDVTGTAVFTHSVADG
                     PEEGTYEIHITGNFDDVSVTGTVDGEPVDSSEVPDFASMEPIDVTVVVDDQGNVVSAG
                     GEDVENPLGGMLGDLGPVGGGAPAPGLDPGQFFGPLFSDGEVAVGDSWSDEIETPGMG
                     EESVVTTVTNTITGIEQFDGAEVYVIDTTSTTSPIEFDLGEFFKGMFGAFAPEGATPE
                     ETAEFDEMMSQLKFVISVDDTTADGTTRFDAEAGVARQSEINAATHIAMDMKIPDETT
                     GEVMSFQMEMTMDQGITYHLISGPQA"
     gene            6989..7942
                     /gene="deoC"
                     /locus_tag="AC161X_08645"
     CDS             6989..7942
                     /gene="deoC"
                     /locus_tag="AC161X_08645"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014742742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HIR4989660.1"
                     /translation="MTLAPARTSILPDRITTPTLRSFLRSVPSVDEVGANERVAALST
                     RSIKRESKRAALDLITRMIDLTTLEGKDTPGKVAQMSAKAIRPDPMDPSVPSVAAVCV
                     YPNMVKYAVEATYGSTVKVASVATYFPSGLVETDLKVEEVTRVVRLGADEVDMVIDRG
                     AFLGGEYFKVFDEIRRVKDACGDAHLKVILETGELVTLDNVRQASLLAMAAGADFIKT
                     STGKVQPAATLPVTLVMLESIRDFMDATGVMVGMKPAGGIRTAKDAIRYLVVLNETLG
                     EPWMSPDWFRFGASTLLNDVLMQVRFLETGRYSSPDYFTKD"
     gene            7981..9423
                     /locus_tag="AC161X_08650"
     CDS             7981..9423
                     /locus_tag="AC161X_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007455491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR4989661.1"
                     /translation="MKWEYAGSRESAEIASIEDRYGLFIDGEFVKPNSGNYFPTINPA
                     TEEHLAEVAEAGVADVDDAVTAARRAYTDVWGPMAGAERAKYVFRIARMIQERSRELA
                     VLETLDGGKPIKESRDVDLPLVASHFFYYAGWADKLEYAFPGVRPRPLGVAGQVIPWN
                     FPLMMLAWKVAPALAAGNTVVLKPAETTPLTALVVAQIAAEAGLPPGVLNVVTGAGAT
                     GAAVVGHADVDKVAFTGSTAVGKLIASQLVGSHKKLTLELGGKAAHVIFEDAPLDQAI
                     EGVINGIFFNQGHVCCAGSRLLVQENIHDRFVDKLKRRLSTLRVGDPMDKNTDVGAIN
                     SRAQLEKITELVDSGLDEGAEMFQPDCVLPDRGFFFKPTLFTNVAQSHRIAQEEIFGP
                     VLSVLSFRTPEEAVEKANNTPYGLSAGVWTSKGSRILWMADRLQAGVVWANTYNKFDP
                     ASPFGGYKESGFGREGGRHGLLAYMDWDET"
     gene            9420..10268
                     /locus_tag="AC161X_08655"
     CDS             9420..10268
                     /locus_tag="AC161X_08655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013492054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR4989662.1"
                     /translation="MSRLDVRKTYKLYIGGKFPRSESGRSYPALSGDGEVLARVAQGS
                     RKDLRDAVRVAREAVPGWAGTSGYNRGQVMYRIAETLEDRRGTFVEQLHAGGLANKAA
                     DAEVAAAIDRMVWYAGWADKFAQIYGNLNPVAGPFFNISSPEPTGVVGVVAPEEPALL
                     GLVSRLAPVLVPGNTAVVLASERYPMPAITFTEVLNSSDVPGGVVNVLTGLKDELIPW
                     LADHMDVNAIDAGGASPEQAIRVQEGAVHNVKRVVLPSTTGPGQSPYLIAAFTETKTV
                     WHPKGL"
     gene            10423..10983
                     /locus_tag="AC161X_08660"
     CDS             10423..10983
                     /locus_tag="AC161X_08660"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4989663.1"
                     /translation="MPPIPSLTDREFSSYLRAARAGDEGAWRALYDWLAPQILGFLRS
                     SGLPDPDDVLGDVFLDLARGVKDFKGNAKGFRALVFTIARARRVDEFRRLSRRSEEPL
                     DAVIHDLAPAGSDVEHEALALVALDDLLEILGQLTDDQAEVLVLRALGGWTSREIGEI
                     TGRSTGAVEQLQHRATEALRDLLEAP"
     gene            11014..11781
                     /locus_tag="AC161X_08665"
     CDS             11014..11781
                     /locus_tag="AC161X_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989664.1"
                     /translation="MDPGESRSVVAFLTSLVDQVAVAPDEVTAYRHISAAAATARGLV
                     VRGSRSFRWVAAATAMVTALGTGGVAMAGGLPDPIQTAVADAARVLPLPIPVPYPHAR
                     LSDPAPVDHPEVDTWSRPAPAEAMADEPEDSGEAPVVSAAGDDSSERPHLAVASDQRS
                     CDFDGERRHEGKERDSACDELVAPPGFEQDVDRFDGDHDEDRDEEFVDRDRESRADRL
                     SEARDDDSDHRGQDESDRQEERHDHESPDDDSSTGRN"
     gene            11817..12728
                     /locus_tag="AC161X_08670"
     CDS             11817..12728
                     /locus_tag="AC161X_08670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989665.1"
                     /translation="MLTIRGRSKALITGLVLTLMATLPGIAQAENNTTEETTSTTTPV
                     PPVTQVTSVLPVLGSGLTVSLTRDAAGVITEVALDPSGATIVKEGDHKVVFLLADGDT
                     QVVVKSYSKGKGDDATAGGVKTTVTADATADVTGPGSWSADVFGTGVVTIPYTVAFDG
                     NVPSITIGDITLPDGVTAEISEPKIKTSDEKSKFRIKVTLISGDNKARVSFLASTRLN
                     EDGELKVKLAVTLDAKNHVRCGWGDGDRDHRDDDDNQVGGDEESGRRWESRNHEERDH
                     DRENRRDREDGDHDGGRHGGGDDDGEG"
     gene            12776..13417
                     /locus_tag="AC161X_08675"
     CDS             12776..13417
                     /locus_tag="AC161X_08675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989666.1"
                     /translation="MNWALRPLLALVVACSTTGGVVTSTSLVATTTTVAPTTSVPATT
                     TSTQAIDVAGEFAAALAATSPNYRFESVVMVGDQTITTIAGVVDGESVAATITAGTSD
                     VSYVRTPEGEWVTGPDDEWSVLEGEAPAGAPLAGLADVSDLALESSDAGLLAVLATLG
                     PAAGPVAGTRVTITLTDGQVTQIRYQAQSGSDLADVTTTITEVGSAGTVEVPV"
     gene            complement(13374..14180)
                     /locus_tag="AC161X_08680"
     CDS             complement(13374..14180)
                     /locus_tag="AC161X_08680"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4989667.1"
                     /translation="MLEATGISHRVNGVVTLADTSIEVKAGEFVAIVGPNGAGKTTLM
                     AMLAGELEPSTGTVTLAGKPLKDTTLEERSRLRAYLPPMQTQEIGFTVREVVAMGRHP
                     WRDQDLPDDDLTEAALEAAGVASLAGRKLGSLSTGEAQLVQIARIFSQQAALLLLDEP
                     TGGLDISHQERILRALASRADHHSVVAVLHDINAAASVADRMLIMSGGKAVAFGSPAQ
                     VLNEDLLSDVYDHPISVISHPFRDGPLVLVGNQTSDVRPAPRPSPQTPPR"
     gene            complement(14180..15169)
                     /locus_tag="AC161X_08685"
     CDS             complement(14180..15169)
                     /locus_tag="AC161X_08685"
                     /inference="COORDINATES: protein motif:HMM:NF013219.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FecCD family ABC transporter permease"
                     /protein_id="HIR4989668.1"
                     /translation="MKGRWALLVGILIGVSVLSLGIGAVSLSLPEVWNGVLTGVGLGS
                     APETATAVIWELRMPRTLAALLVGGGIAAAGVGLRGIYRSPMAEPYLLGISPAAGLGV
                     VIGSLFTPIGVPPVAGAILGAATAVLMALGIHRLTAAVGAGNRLILLGVALGLTFLAW
                     TVTVIFVGDSPRLPTFTYFVFGSFAAVTWSHFLVALPLIAAGAAIIGVRGRALDLMSL
                     GDAPARSLGVEVQRTTTWVLVAAGIAAGASVALAGVIGFVGLIAPHIANRIVGPRHGR
                     SLPVAIAVGGTLLLICDIASRLVANPVEVPVGIVVAAIGGPALVGVLLQSRQA"
     gene            complement(15166..16098)
                     /locus_tag="AC161X_08690"
     CDS             complement(15166..16098)
                     /locus_tag="AC161X_08690"
                     /inference="COORDINATES: protein motif:HMM:NF013648.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme/hemin ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR4989669.1"
                     /translation="MDDLPGHVLRDHVLRQGGLPHWMRRFVALVVVFSALIASCSQPE
                     EAVQLVDSTRIVSLSGDITEILFELGVGERVVGVDVTTVAPEAATQLPIVGVGRFLTA
                     EGALSAEPTLVIGDTQTAPATTLDQIRAAGVKVEILDVPTSFDDLYQKIRVIGELLGR
                     EEQAEDLVTRLEAEVEDASAGALTTSPRVAYVYTRGPDVNLLFGDGMTTRPLIEAAGA
                     VDAGSEVGIVGTSSVTAEALVSAAPDVIVVPAEGVELLGGIEAFLELPGVGQTPAGER
                     GAILAYPEGDFLTFGPRIADSLRLLIEDLAALQN"
     gene            complement(16029..>16509)
                     /locus_tag="AC161X_08695"
     CDS             complement(16029..>16509)
                     /locus_tag="AC161X_08695"
                     /inference="COORDINATES: protein motif:HMM:NF053415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MTB12 C-terminal domain-containing protein"
                     /protein_id="HIR4989670.1"
                     /translation="TTTTVEEASTTTTLGQTTTTLAPETTTTAAVETTTTAPAPIVPG
                     EDADVDAVVAIYGIAFDSATTFDEKAVVIVDPAGLEETVTAYATTGEAMGGVTTQVDA
                     VVIDGDNATVTYTLMFAGTPTYSGLQGDAIRTADGWMISRDMFCGIMSSARVGCPTG"
BASE COUNT         3122 a         5221 c         5342 g         2824 t
ORIGIN      
        1 ggcctttggc gcctactcgt aggtcagtcc gccgatgtcg ggaacgagtg agatccagac
       61 ctggccgggt gggacggcga tgttgttgcc atcggcgtcg gtcaacgtgt accaatcctg
      121 ctcggtcgca cgctcccagg tcccctcgat gaccttgccg tcggcgaaca cccaggcctg
      181 gccggacccg gtggtgtgcg agctcggaag gccgttgttc gagtactgct cagattcgag
      241 gccgatcagc accgggaacc cgatccggcc ctcggtgtcg tcttcatcgc gccagttcga
      301 ctcccgaccg tctgcgctgc gtagccaggt tgcggtggtt ggatcccagg tccagtcaac
      361 gtcggttccg cggtaatcga tgtggaccgc actggcggca gcggcatcgg tgggcatggg
      421 tccgaattcc cagatcggcc cggtcggggg aaggtcttcg tagtccctgg cgtcagcatc
      481 tgcccgcaga cctgcagtgg tcgtgtagag attgtgcggg gcccgtcgga acggcacccg
      541 gaacgcggct ggcgtggtct cggtgagcag attgatgtct ttggacctga tcatttcctg
      601 gacccaaccc tgggcgccgg agatggcgaa ggtcggtttg ttgagcgcgg tgagcaaagt
      661 tgagtccgtg ggacgtcccg atcgcatcgg gcccaggaac tcggcgtctg actgcatcca
      721 catcgtcagg aacctggaaa tgccttcgac ccggatctcg aagacggcat cggcatgatt
      781 gatgcctgac tgcggtctgg cgttgggatg gttgtcgatc ttcaccgcca acaaccttcg
      841 gttgaggagt gcgggatcgg caaccgggag cccattgatc ggtgagagcg tggttgcctc
      901 agtcgtcgtt gtccccaccg aagaggtggt cgtggccagg gttgtggtgg tcgatgtgga
      961 ggtcgtcgtg ctgtcgattg cctccgccgg cgagcaggcg gcgaggatca acaatgacac
     1021 gacgaactta ctgaaggttg aacgcattgg cggcagcgta tctctacccg ccggctacac
     1081 ggttaatacc cctccggtga ccgggagttg aggcatggag ctttcaggct cgtcgatgga
     1141 cactgcgagc tctattcctt cggcgctgga ccgcaggaca tagagggcgt cgatgttgat
     1201 gccggcgtcg gcgatgaccc gggtgagccg ggcaagctcg ccgggacgat gggggagttg
     1261 cgcagtgagc accatgcgct cctcgctctg aagggcggcc tcgctcaaca cccggcgggt
     1321 ggtggcagcg tcgtcgacga tgagacggac ggttccctcc ccgttggttc cgtaggcagt
     1381 gagagcttcg atgttgaccc cgaaggctgc gagggcctcg gtcaaagagg cgagtcggcc
     1441 cggacggttc tccatctgga cgatgaactc ggtcatggtt cgactctacg ccgggtcgtt
     1501 caccacgtgt ccggcgaccg ggtccggtgg ctcgacccgg ccccgcgttg ataggtagcg
     1561 atgccagatc agccgggcgg cgacggcgcg caccgggcgc cacggtgcgg agatggtctc
     1621 cagctctctg gggccgggca ccgcatcgag gccaagcgcc tccatggtcc ccacctggag
     1681 ggcacgatcc cccaccggga agacgtcggg gaggcggagg gcggacaaca agtaggcgtc
     1741 ggccgtccac agcccgattc ccttgagtgc gatcaggcgg gctcgagcgt cgggctcggt
     1801 ggtctggggc aatgcatcca agtcgagctc gcctccagac acggcttcgg ccagcccgag
     1861 caggtatccc accttctgac gggtgacccc gatggagcgc aatccggtgt ctccggcttc
     1921 gagcaccgtt ttcgccgtca ccacatcaac caggtcgaga agacggcgga acatcgccgc
     1981 tcccgattcc agggagacct gttgctccag gatcaacagg accagggtgg agaacccggg
     2041 ctggcgttgc cagaagggag gcagccccca ttcgtcgacg atcaggccca gagccggttc
     2101 ctcatcggcg agcaagatgg ccgcgtcccc aaaggtgccg gcatcgaggc tggtgactct
     2161 gacccacaag gaggcgaagg ggatcggccc gagggtgcgc gagtcggctc gggtggcatc
     2221 gaggttgtcc gagatcaccc aggcctcgtc ttcccccagc cggtggcctc cggcctcctc
     2281 gcccggcccg gcagccatcc gtttcaccag ccagaagttg tcgcgggagg ggtgggggaa
     2341 gacaacaacg tcaccgctcg caggcaggcg ggcgtcggtg gcaacgaact catcacctgg
     2401 cgcgagaccg ggcagcatcg actcctcaac gaccacgaac ctgcggattg acatcagacg
     2461 ggaagggtat ggtcggcgac acagaacgac caacaaggag tgtcaatgcg ccagctaact
     2521 gtcgcccacg cccattgcga cctctactgc ggggtctacg acccggccca ggccaagatc
     2581 gaggcgatgt cggtcctcaa gatcgccgag aagtacgcct catccgacga tgaggtgttc
     2641 aaggcgagag cgatcagcct gaaggaggag cgggccgagg aggtcaagca ccatctcatg
     2701 gtgctctggg ctgacttctt cacggccgag caccgcaccg aatttcccaa cctcgatgac
     2761 ctcttctggc gtgccatcca ccaggccggt gacgcgaaga agtcagtcga tcccgaagac
     2821 gggcggaaac tgatcgcgtt gatcgacgag atctccgacg tgttctggca gacggagaag
     2881 gcgaagggga tgggggtcta cccgccggcc tgattcaggc ggagggctga gtcaagcttc
     2941 gccacaacct ggtcatcgcg gccttgatcc ggtcgagcat ccgctgcgcc caagggaact
     3001 cctggccgat cagagccagg ccgccaagga tggtgaggac accggggccg ggcagcacga
     3061 gcatcgcgat ccccgcgatg aggaggatga agccgcccac gacgcgagcg atccgtgtca
     3121 tgagggcaaa cgtagtcgcg gttggagtct tggcgaatgg ggtttcaccc tgattggacc
     3181 ccgatggacg tcctggagtt tcaatcagtc cagggcgtcg tcctccaact tggcgaaaag
     3241 cggctggatt tcgccaaggg cggtcccggc cgacacctcg ggacgcttcc aactcgcgac
     3301 cgcgccaggc agacccagca tctcccccac cttcggcgtg gtgaacggga gatacggaga
     3361 cagggccacc ctgatgcccg agatcgcctg gatagcggtc cataagatcg tctcggcacg
     3421 tttcgggttt tccttgacca acttccaggg ggcgttggcg ttgagataga cgttgacctc
     3481 ggtggccgcc tccatggcgg cacgcaatcc ggcacgcaac tccacggctg cgatgtgccc
     3541 ggccacggct tccagggcct ggtccacgtc cgcaagcagt gcctggtctt caccggtcag
     3601 ttcgtcgggt tcgggcacct tgcggtcgaa gttgttggcg gtcagtgaga ggacccggtt
     3661 gaccaggttg ccccaggtgg ccaccagctc atcgttgatc cggcgaatga tctcctcatc
     3721 actgaggtcg gtgtcgttct gctcggggag aaccgaggct atggcatagc gcaacgcatc
     3781 gggctcgaga cgctcggcat accagccgat cgaccgtccc acccccttcg atttcgaggc
     3841 cttggcgccc ttgaaggtga tgtactggtt ggccgggacg tcggttggga ggttcagccc
     3901 gccgtagccc aggagcattg ccggccagat cacggtgtga aacgggatgt tgtccttgcc
     3961 gacgaagtag taggactcgg cctcggggtc ctcccaccaa ttgcgccacg cctccgcgtc
     4021 accgctgttc tgggcccact ctttggctgc ggacagatat ccgatgaccg cgtcgaacca
     4081 cacatagatc cgcttccccg gccccagatc gtcgaccggg acctccacgc cccattcgat
     4141 gtcacgcgtg atcgcccgat cgggcatccc ctcttcgatc atccccagtg cccagttctt
     4201 cacatgacgg cgccaggtgg tcttggattc caaccaagcc ttgaggggtt ctgcgaactc
     4261 ggggagtctg aagaagaaat gctcggtctc gcggaggacc ggtgtcgcgc cactgaggcg
     4321 ggacctcgga ttgatgagat ccgtggcatc gagggtgcgc ccgcagttct cacactggtc
     4381 gcccctggcc tcggtgtacc cgcaatgggg acaggtgccc tcgacatacc gatcggggag
     4441 gaaccgtccg acctccgggt cgaagaattg ggacgaggtg cctttgacca gatggccctt
     4501 ttccaagagc ttcaggaaga cgtcctgggt cactgcgtgg tggttctcgg tcatcgtggt
     4561 cgtgaacagg tcgaacgaga tcccaagcgc ctcccaatag ccgaggatct cggcgtgata
     4621 tttgtcaaca acttctcgtg gcgtgatgcc ctcttcgtcg gcgcggacgg tgatcggtgt
     4681 gccgtgggcg tcagagcccg agaccatgag cacgtcgtct ccggcgaccc ggtggtagcg
     4741 ggcgaaaatg tccgatggga gataggcacc gccaaggtgc ccgagatgca gcgagcccga
     4801 tgcgtagggc caggcgacgg cgacgaggat tcggcgagtc attggagccg gagggtaaac
     4861 gccaccgggc cctcagcctg cggtgtcgat gccgggcacg gggacatggc gactcgaccg
     4921 ctcggtcaac accactccac caatgatcag gacggcgccg atgagaacag ccagagtgag
     4981 cgtttcgccg aggaagatga cggccagggt gaccgccagg atcggcacca gatacccggc
     5041 caccgccaat cgggatgcgg gattcacctg cgccgcgatc aggaaggctg tgaatggaac
     5101 ggcagtcgcg aacgaaccga gaaggatcag cagcacccaa tccgacgatt cgagggaatc
     5161 cagcggggtg gcatccagga acggggtcgc cagcagtgag atcgccgtgg caaaggcgaa
     5221 ttgggggatg accaggccgt ccccgcctac ttccagggca aagcgacggg ttagcgctcc
     5281 gccaattccg gcaaacaaga ccgcaatcaa cgaaagggtg accccgagcc cgagattgcc
     5341 cacaccctcc agcgatcctc caccgactcc gaccaggatc ccggtcccga tcagcgacac
     5401 gatcagcccc ggcgtggatt tggggttgaa tcgctcgccc tcgaccagaa aatgggcggc
     5461 tccgactgtg gcggccggga tcagggcgac cattagcccc gacaggctta ccggcaactc
     5521 gaccaggccc atggtgaaga aggtgttggg aatggccaac gcgacggcac cgagtcagaa
     5581 gcctctcacc cagtgcccac gatgaagctc gcggaagcgc acccaataga tcgagaccaa
     5641 caagatgaca atcagggcga ccccgaagcg gaacggaacc acggtgaagg ccgaaacccc
     5701 gttgttcagc acggcccgcg tcaacacggc ggccgagccc catcccagga tcgagaccac
     5761 gattggggtc cacgggttca tggtgcgggc acgctaaccc tgccccgggt tagtcctaaa
     5821 aggaaagccc ctgaatgccg ataacctcac caacatgatc aagcgctcac ttttcctcgt
     5881 tgcgatcctg gccatggtcg tgaccgcctg cgggggcacg gtcgagactt cgaccaccgc
     5941 cgccgccgac agcaccacga ctgctccacc agccgaggcg gtcctgctca gctactcgct
     6001 gaccgctggt gacgagttca agtacgaggt aggcctcgac cagcacatcg agctcaccac
     6061 ctccggcgac gcctccgcaa tgggtgaaga ggacgtcccc gaagcggctt cagtcgacgt
     6121 gacgggaacc gcagtgttca ctcactctgt ggccgatggc cccgaggagg ggacgtatga
     6181 gattcacatc accgggaact tcgacgacgt ctcggtgacc ggcactgtcg acggcgagcc
     6241 ggtggactcg agtgaagtgc ctgactttgc gtcgatggag cccatcgacg tcacagtggt
     6301 cgttgacgac cagggcaacg tggtcagcgc cggcggcgaa gacgtcgaga acccgcttgg
     6361 cggaatgttg ggcgacctcg gtcccgtggg gggcggtgct ccggcacccg gcctggatcc
     6421 cgggcagttc tttggccctc tcttctcaga tggcgaagta gctgtcggcg actcctggtc
     6481 cgatgagatc gaaactccgg gcatgggtga agaatcggtg gtgaccaccg tgacgaacac
     6541 catcaccgga atcgagcagt tcgacggcgc cgaggtgtat gtgatcgaca cgacatccac
     6601 cacctcaccg atcgagttcg atctgggcga gttcttcaag ggcatgtttg gggcctttgc
     6661 ccccgaagga gccacccctg aagagaccgc tgaattcgat gagatgatga gccaactcaa
     6721 gttcgttatc agcgtcgatg acaccacggc agacgggacc acacgttttg atgcagaggc
     6781 cggcgtggct cgccagtcgg aaatcaacgc tgccactcac atcgcgatgg acatgaagat
     6841 ccccgacgaa accaccggcg aggtcatgtc cttccagatg gagatgacga tggaccaagg
     6901 gatcacctac cacctgatct ccggcccgca ggcctagcag tcagcccgtc ggcgtagccg
     6961 gggagggggg cgcccctaga attcggttgt gaccctggcc cctgcccgca cctcgatcct
     7021 gcccgatcga atcacgacgc caacactgcg ctcattcctg cgatcggtgc cctccgtgga
     7081 cgaagtcggg gccaacgagc gggtagccgc cctttccacc cggtcgatca aacgtgaatc
     7141 gaagcgagcc gccctggatc tgatcacccg aatgatcgat ctgaccactc tggaaggaaa
     7201 ggacaccccg ggcaaggtcg cgcagatgtc tgccaaggcg atccggcccg acccgatgga
     7261 ccccagcgtt ccctctgtgg ccgcggtatg cgtctatccc aacatggtca agtacgccgt
     7321 cgaggccact tacggcagca ccgtgaaggt ggcgagtgtc gccacctact tcccatcggg
     7381 cctggtggag accgatctca aagtcgaaga ggtaacgcgg gtggttcgcc tcggtgccga
     7441 cgaggtggac atggtgatcg atcgcggcgc tttcctgggc ggcgagtact tcaaggtctt
     7501 cgacgagatc cgtcgggtca aggacgcctg cggtgacgcc catctcaaag tgatcttgga
     7561 gaccggggag ttggtcaccc tcgacaacgt ccggcaggcc tcgttgctcg ccatggccgc
     7621 cggggctgat ttcatcaaga cctcgacagg caaggtccag ccggcggcca ccctcccagt
     7681 gaccctggtg atgctggaat cgatccgtga cttcatggac gcgaccgggg tgatggtggg
     7741 tatgaagccc gctggcggaa tccgcaccgc caaagacgcc atccgctacc tggtggtcct
     7801 caatgagacc ctcggtgagc cctggatgtc cccggactgg ttccgtttcg gcgcttcgac
     7861 tctcctcaac gatgtcttga tgcaggtccg attcctggaa accggccgct actcctcacc
     7921 cgactacttc accaaggact gaacgtggca atcgagatcg cgaacccggc tcacgacccg
     7981 ttgaagtggg agtacgccgg ctcccgggag tcggcggaga ttgcttccat cgaggatcgg
     8041 tacgggctgt tcatcgatgg cgagttcgtc aaacccaact cggggaacta cttcccaacg
     8101 atcaatcccg ccaccgaaga gcacctggcc gaggtggcgg aagccggggt ggccgacgtg
     8161 gacgatgcgg tcacggcggc ccggcgcgcc tacaccgacg tttgggggcc gatggcgggc
     8221 gccgagcggg ccaaatacgt gtttcggata gcccggatga tccaggagcg gtcccgggaa
     8281 ctggcggtcc tggagaccct ggatggcggc aagcccatca aggaatcacg tgacgtggat
     8341 ctccccctgg tggcgagtca tttcttctat tacgcgggat gggccgacaa gctggagtac
     8401 gccttccccg gggtgaggcc gaggcccttg ggggttgccg gccaggtgat cccctggaac
     8461 ttccccctga tgatgcttgc ctggaaagtt gccccggccc tggctgccgg aaataccgtg
     8521 gtcttgaaac cggccgagac cacccccctc actgccctgg tggtcgccca gattgctgct
     8581 gaggccggtc tgccgccggg ggtgctcaac gtggtgaccg gggcgggagc gaccggcgcc
     8641 gccgtggtcg gccacgctga tgtcgacaag gtcgccttca ccggatcgac cgccgtcggc
     8701 aagctgattg ccagccagct cgtcggcagc cacaagaagc tgaccttgga gttggggggc
     8761 aaggccgccc atgtcatttt cgaggatgct ccactcgacc aggccatcga aggcgtcatc
     8821 aacggcatct tcttcaacca gggccatgtc tgctgcgccg ggagccggct tctggtccaa
     8881 gagaacatcc acgaccgctt cgtcgacaag ctgaagcgtc ggctgtccac cctccgcgtc
     8941 ggtgacccga tggacaagaa caccgatgtc ggagcgatca acagcagggc acagctggag
     9001 aagatcaccg aattggtcga ctcgggcctc gacgaaggcg ccgagatgtt ccagcctgac
     9061 tgtgttctcc ctgaccgcgg cttctttttc aagccgaccc tgttcaccaa cgtcgcccaa
     9121 tcgcatcgaa tcgcccagga ggagatcttc ggcccggtcc tctccgtcct ctcattccgg
     9181 actccggaag aagcagtgga aaaggcgaac aacaccccct acgggctgtc ggcgggggtg
     9241 tggacctcga agggctcgcg gatcctatgg atggccgacc gcctccaggc cggggtggtg
     9301 tgggcgaaca cctacaacaa gttcgacccg gcttcaccgt tcggtggcta caaggaatcg
     9361 ggattcggcc gggagggcgg ccgacacggc cttctcgcct acatggactg ggacgagaca
     9421 tgagccgtct cgatgttcgc aagacataca agctctacat cggaggcaag tttccccgtt
     9481 ccgagtcggg ccgttcctat cccgcactca gcggcgatgg ggaggtgctg gcccgtgtgg
     9541 cccaggggag tcgcaaggac ctcagggatg cggtgcgggt ggcgcgagag gcagtgccgg
     9601 gttgggccgg gaccagcgga tacaaccggg gccaggtcat gtaccggatc gccgagacgc
     9661 tggaagaccg gcgggggacc ttcgtcgagc agcttcatgc cggcgggctt gccaacaagg
     9721 ccgccgacgc cgaagtggcc gccgcgatcg atcgcatggt ctggtacgcc ggctgggccg
     9781 acaagttcgc ccagatctac ggcaacctga atccggtcgc cgggccattt ttcaacatct
     9841 cctcgcccga gcccaccggt gtcgttggag ttgtggcacc agaggagccg gcattgctcg
     9901 ggctggtgtc gcgactggct ccggtgctgg tgcccggaaa taccgcggtg gtcctcgcct
     9961 cggagcgcta cccgatgccg gcgatcacct tcaccgaggt cctcaacagc tctgacgttc
    10021 ccggcggggt agtcaatgtc ctcaccgggc tcaaggacga actgatcccc tggctcgccg
    10081 accacatgga tgtgaacgcg atcgacgccg gaggggcaag cccggaacag gcgatccggg
    10141 tgcaggaggg tgcggtccac aatgtgaagc gggtggttct gccttcgacg actgggccgg
    10201 gccaaagccc ctatctcatc gcagccttca ccgaaaccaa gacggtctgg caccccaagg
    10261 ggctctagaa acgggatgtc ccctggcatg gggtatgacc ctttgggctc ctgggtagag
    10321 ggtgtgtcgg ccttcgccct tgacattggg cagcgactcg tgttcatatg agatcgggcc
    10381 tgggaggccg tgcgagctca gctcgaccga ccaaggagtc cagtgccccc aatcccaagc
    10441 ctcacggatc gggaattctc gtcgtatctc cgtgccgcgc gcgcaggtga tgaaggtgcc
    10501 tggcgggcgc tctatgactg gctcgccccc caaatcctcg gctttctccg ttccagcggc
    10561 ttgccggacc ctgacgacgt gctcggtgac gtcttcctcg atttggcccg tggtgtcaag
    10621 gacttcaagg gcaacgccaa aggtttccga gccctcgtgt tcacgattgc ccgagcgcgg
    10681 cgtgtcgacg aattccgtcg tctctcgcgc cgctccgaag agcccctgga cgccgttatc
    10741 catgaccttg cacccgccgg gagcgacgtc gaacacgaag cattggcatt ggtcgcgctc
    10801 gacgacctgc tcgagatctt gggccagttg accgatgacc aagcggaggt tctggtgctt
    10861 cgcgccttgg gtggttggac ctcgagggag atcggagaga tcaccggtcg cagcacaggt
    10921 gcggtggagc aactccagca tcgggcaacc gaggctctgc gggatctcct cgaggcgccg
    10981 taaggaatca cagcttccca acgattccct ggcatggacc caggagaaag tcgcagcgtc
    11041 gtcgcattcc tgacttcgct ggttgaccag gtggccgtgg cgccggacga ggtgaccgcc
    11101 tatcggcaca tctcagctgc cgccgccacc gcccgaggtt tggtggtgcg tggctcgagg
    11161 tcatttcgct gggtcgcagc tgccaccgcg atggtcacgg ccctcggcac cggcggtgtt
    11221 gccatggcgg gcgggctacc cgacccgatt cagaccgcag tcgccgacgc cgcccgggtt
    11281 ctcccgcttc ccatcccagt gccttatccc catgcccgac tttcggaccc agcaccggtc
    11341 gaccacccag aggtcgacac ctggagtcgg ccggcgcccg ccgaggcgat ggccgacgag
    11401 cctgaagact caggcgaagc gcctgtcgtt tcggccgccg gtgacgattc gtccgagcgt
    11461 cctcacctgg ccgtcgccag tgaccaacgg tcatgcgact tcgatggaga acggcggcac
    11521 gaaggcaaag agcgcgactc ggcctgtgat gaattggtgg cacctcccgg gttcgagcag
    11581 gacgtggacc ggttcgacgg cgaccacgac gaggaccggg atgaggaatt cgttgatcgg
    11641 gaccgcgaat cgcgggcgga caggttgtct gaagcccgcg acgacgactc cgaccacaga
    11701 ggtcaagacg aaagcgatcg acaggaggaa cgacacgacc acgaatctcc ggacgacgac
    11761 tcgtccacgg ggcggaactg accgccactg aacttccctc gagagaaaga gaccgaatgc
    11821 tgacaatcag aggccgatcc aaggcactca tcacaggcct ggtgctgacc ctcatggcga
    11881 ccctgcccgg gatcgcccag gccgagaaca acaccacgga agagacaacc agcacaacca
    11941 ccccggttcc cccggttacc caagtgactt cggtgctacc ggtgctgggc tcgggtctca
    12001 cggtatcgct gacccgtgac gcggccggtg tgataaccga agtggccctg gatccatccg
    12061 gcgccaccat cgtcaaggaa ggtgaccaca aagtcgtgtt cctcctcgcc gatggagaca
    12121 cccaggtggt cgtcaagtcg tactcgaagg gcaaaggcga cgatgccacc gcaggtgggg
    12181 tcaagacgac cgtcaccgcg gacgccacgg cagacgtgac cgggcccgga tcctggtctg
    12241 cagatgtgtt tggtaccggt gttgtgacca tcccctacac cgtggccttc gacgggaacg
    12301 tcccttcgat caccattggt gacatcacgc ttcccgacgg cgtgaccgcc gagatcagcg
    12361 agcccaagat caagacctca gacgagaagt cgaagttccg gatcaaggtc accctgattt
    12421 cgggagacaa caaggcccgg gtcagcttcc tggcttcgac ccgtctcaac gaagacggtg
    12481 agttgaaggt gaaattggcc gtgaccctcg atgccaagaa ccacgtccga tgcggctggg
    12541 gcgacggaga tcgtgaccat cgtgatgacg atgacaacca ggtcggcggc gacgaggagt
    12601 cggggcgtcg ttgggagtcg cgcaatcacg aggagcggga ccatgaccgt gagaatcgac
    12661 gtgatcgcga ggacggcgac catgatggtg gtcgtcacgg tggcggcgat gatgacggcg
    12721 aaggctgagg cggatgattg aaaaacctgg ctcagaccag tctgtcggag ggcccatgaa
    12781 ttgggccctt cgaccgcttc tggcccttgt tgtggcgtgc tccaccacgg ggggcgtggt
    12841 cacctcgacg agcctcgtcg caaccacaac cactgtcgcc ccgaccacgt ccgttccggc
    12901 caccaccact tcaacgcagg ccatcgatgt tgccggcgag tttgcggcgg ccctggccgc
    12961 gacatcgccc aactatcgct tcgagtcagt ggtgatggtt ggcgaccaga cgatcacgac
    13021 gattgccggc gtcgtggacg gtgaatctgt ggcggcgaca atcaccgccg gcacgtctga
    13081 tgtctcttac gtgagaacgc ccgaaggcga atgggtgacc ggccccgatg acgagtggtc
    13141 ggttctggaa ggggaggctc cggcaggcgc tccgctggcc ggtctggccg atgtcagtga
    13201 tctcgccctc gagtcatctg atgccgggct gttggcggtc ctggccacat tgggccccgc
    13261 ggccgggccg gtggcgggca cccgagtgac catcacgctg acggatggcc aggtcacgca
    13321 gatccgatat caggcacagt cggggtccga cctcgccgac gtgaccacca ctatcaccga
    13381 ggtggggtct gcggggacgg tcgaggtgcc ggtctgacat ctgaagtctg gttgccaacc
    13441 aagaccaaag ggccatccct aaacggatgg ctgataaccg agatcggatg gtcatagaca
    13501 tcgctgagca ggtcctcgtt gagcacttgc gccggtgagc caaaagcaac agctttccct
    13561 ccagacatga tcagcatccg atccgccact gacgctgcag cattgatgtc gtggaggact
    13621 gccaccaccg aatggtgatc cgctcggctg gccagggctc gcaggatccg ctcctgatgg
    13681 ctgatgtcca gccctccggt cggctcgtcg agcaggagga gtgccgcctg ctggctgaag
    13741 atgcgggcaa tttgcaccag ttgcgcctcc ccggtggaca acgagccgag ctttcgtccc
    13801 gccagggacg ccactcctgc cgcctccaac gctgcctcgg tcaaatcgtc gtcaggtagg
    13861 tcctggtcgc gccacggatg gcgccccatc gccacgacct cacgcaccgt gaagccgatc
    13921 tcctgggtct gcatcggagg caggtaggca cggagtcgag atcgctcctc gagcgtggtg
    13981 tctttcagag gcttacccgc caaggtgacc gtcccagtcg agggctcgag ctcaccggcg
    14041 agcatggcca tcagggtcgt ctttccggcg ccattggggc cgacgatggc cacgaattca
    14101 ccggctttca cctcgatcga agtgtcggcg agcgtgacga ctccgttgac ccggtgcgag
    14161 atcccggtcg cctcgagcat caggcctgcc tcgactggag caacacaccg accagagcag
    14221 gtcctccgat ggcggcaacg acgatcccta ccggcacctc gaccggattg gccaccagcc
    14281 gggatgcgat gtcgcagatg aggaggaggg tgccaccgac agcgatcgcg accggaagcg
    14341 acctgccgtg gcgaggcccg acgatccggt tggcaatgtg tggagcaatc aggccgacaa
    14401 agccgatgac cccggcgagg gccaccgagg ctccggccgc gatgccggct gccaccaata
    14461 cccacgtggt ggtccgttga acctcgacac cgagactgcg ggcgggggca tcgccgagcg
    14521 acatcaagtc gagtgcacgg ccccgaacgc cgatgatggc cgcacccgcc gcgatcaggg
    14581 gaagtgcaac cagaaagtga ctccaggtca cagcggcgaa gctgccgaac acgaagtagg
    14641 tgaaggtggg caaccgcgga ctgtcaccga cgaagatgac cgtcaccgtc caagccagaa
    14701 aagtgagccc gagagcgact ccgaggagga tgagtcggtt cccggcgccc acggcggcag
    14761 tcaggcgatg gatgcccaac gccatgagaa cggccgttgc cgcccccagg atcgctcccg
    14821 ccaccggtgg cacaccgatc ggcgtgaaca acgacccgat caccacaccg aggccggccg
    14881 caggtgagat cccgaggaga tacggctcgg ccatcgggct gcgataaatg ccgcggagtc
    14941 ccacaccggc tgcagcgatg cccccgccca ccagtagggc tgccagggtg cgaggcattc
    15001 gcaactccca gatcacagct gttgccgtct cgggcgcgga gccgagccca accccggtca
    15061 gaaccccgtt ccatacctcc ggtagggaaa gactgaccgc gccaattccc agactgagca
    15121 ccgacacccc gatcaggatg ccaacgagga gggcccagcg gcctttcaat tctgcaacgc
    15181 cgcgaggtct tcgatcagga ggcgtaggga gtccgcgatc ctcggcccga acgtgagaaa
    15241 gtcaccctcg ggataggcga ggatggcgcc tcgctccccg gccggagtct gcccgacacc
    15301 gggcaactcg agaaacgcct cgattccgcc aagcaactcg actccctcgg ccgggacgac
    15361 gatcacatcc ggcgcggcgc tcaccaaagc ttcggccgtg accgaagatg ttccgacgat
    15421 cccgacctcg gagcctgcgt caacggcacc cgcagcctcg atcaacggtc gggtggtcat
    15481 cccgtctccg aagaggagat tgacatccgg ccctcgcgtg tagacgtagg ccaccctcgg
    15541 agaagtagtc aaggcgccag ctgaggcatc ctcgacctcg gcttcgagtc tcgtgacgag
    15601 atcctccgcc tgctcttccc tgcccaggag ctcgccgatg acccttatct tctggtagag
    15661 atcatcgaag gaggtgggca catcgaggat ctccaccttg acgcctgcgg cgcggatctg
    15721 atccaaggtc gtcgctggcg cggtctgggt gtccccgatg accagcgtgg gctcggcact
    15781 caaggctccc tccgcggtca agaaccgccc cacgccgacg atcggaagct gggttgctgc
    15841 ctcgggggcg accgtggtca cgtcgactcc aacgactcgc tcgccaacac cgagttcaaa
    15901 caggatctcg gtgatgtccc cggataggga gacgattcgt gtcgagtcga caagctggac
    15961 cgcctcctcc ggctgtgaac aggaggcgat cagcgccgag aagacaacga cgagggcaac
    16021 gaatctcctc atccagtggg gcagcccacc ctggcggagg acatgatccc gcagaacatg
    16081 tcccgggaga tcatccatcc gtctgcagtg cgaatggcat caccctggag gccgctgtag
    16141 gtgggggtcc cggcgaacat cagggtgtag gtcactgttg cgttgtcacc gtcgatgacc
    16201 acggcgtcga cttgcgtggt gacaccaccc atcgcctcgc ccgtcgtggc gtaggcagtc
    16261 accgtttcct ccagcccggc cgggtcaacg atcacaaccg ctttctcgtc gaaggtggtc
    16321 gcactgtcga aggcgatccc gtagatagcg acgactgcat ccacgtcggc atcttcgccg
    16381 ggcacgatcg gcgccggggc ggtggtggtc gtctcaaccg cggcagtggt ggttgtctca
    16441 ggggcgagcg ttgtcgttgt ctggccgagg gtggtggtcg ttgaggcctc ttcgacagtg
    16501 gtcgtggtg
//
LOCUS       DBNHXU010000353         2424 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1525537, whole genome shotgun sequence.
ACCESSION   DBNHXU010000353 DBNHXU010000000
VERSION     DBNHXU010000353.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2424)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2424
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1525537"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(2..859)
                     /locus_tag="AC161X_08700"
     CDS             complement(2..859)
                     /locus_tag="AC161X_08700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013734539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR4989671.1"
                     /translation="MTASNASTPHARGFDLGRFLLYAIAISVLLVIVVPLGFSIIGGF
                     RSNAQLAADPVGLPDPWVWTNYQTTFTDPDFYRWVKNSVLVALMATLLVLPAASLAAF
                     VIARYRFRGREIIFAFFTLGLLFPVAVAILPLFITLRQTGLLNNPLGLALPQAAFGLP
                     LSIVILRPFFRGIPRDLEDAASIDGCGPLRFYWSIMLPLSRPVLSTIAVLTIVGSWNA
                     FLLPLLVLTQPGQWTLPLGINNISAQYVTDTAMILAYTTVAMIPALIFYFIAQRQIVS
                     GLTAGGVKG"
     gene            complement(861..1808)
                     /locus_tag="AC161X_08705"
     CDS             complement(861..1808)
                     /locus_tag="AC161X_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015037918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HIR4989672.1"
                     /translation="MTSNSETADPSGPLRRSHRRPGVAPWPMVIGFLLPALLLYGMFV
                     LYPITQSIRYSGYDWNGLEPLTEWVGLDNFREAFADPLFIEAIQHNFIIIGLSLALQI
                     PFAIALAVLLSRSLKGRGLFRTMYFAPFILSEVVTGVVWRQIFRPAGLFDVMLTSAGA
                     GDLTKEWLADPSIALYSLFFVISWKYFGFHMVLIMAGLQTIPDELEEAAAIDGATWWQ
                     GFRFVTLPLLGPTIRVSIFLSIIGALQLFDLVWVTTKGGPVNSTATMATYLVDWGFRR
                     TQFGYASAVSVIIFGLSLVIALLYQRFVLRRDLEGALTN"
     gene            complement(1879..>2424)
                     /locus_tag="AC161X_08710"
     CDS             complement(1879..>2424)
                     /locus_tag="AC161X_08710"
                     /inference="COORDINATES: protein motif:HMM:NF013697.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HIR4989673.1"
                     /translation="AAPWDGPDGESGTIGTGAAAMSLMGQWGPGAYANQSGIGVPEDP
                     IADRLPAEFGWFPFPAVDGGAGAGTDGFGGGDGFAVGKDAEPEAIQFLEFITNVDNAR
                     IVGATGTTLPVTKGAEDSITDPYQLAVLEGLSKATFVQLYLDQYFSPELGAAVNDAVA
                     TLFAGTASPEEVTQAITDAAS"
BASE COUNT          489 a          699 c          841 g          395 t
ORIGIN      
        1 gtcatccctt gacccctcct gccgtgagac cactcacgat ctggcgttgg gctatgaagt
       61 agaagataag ggcgggaatc atcgccacgg tggtataggc gaggatcatc gcggtgtcgg
      121 ttacgtattg cgcagagatg ttgttgatgc cgaggggcag cgtccattgc cccggctgcg
      181 tgagcaccaa gagaggcaag aggaacgcgt tccagctccc gacgatggtg aggacggcga
      241 tcgtgctcag caccgggcgg gagagcggga gcatgatgga ccagtagaag cggagcggac
      301 cgcagccatc gatcgaggcg gcgtcctcga gatcgcgagg gatgccgcga aagaacggcc
      361 tcaggatgac gatcgaaagt gggaggccga aggctgcctg ggggagggcg aggccaagtg
      421 ggttgttgag caggccggtc tgacgcaagg tgatgaacag ggggaggatg gctaccgcga
      481 ccgggaagag aagtccgagg gtgaaaaatg cgaagatgat ctcccgccct cggaaccggt
      541 agcgggcgat gacgaaggcg gcgagcgaag cggcgggcag caccagcagc gttgccatca
      601 gtgcgaccag cacgctgttc ttgacccagc ggtagaagtc cgggtcggtg aacgttgtct
      661 ggtagttggt ccagacccag gggtcgggca gccctaccgg gtcggcggcg agctgtgcgt
      721 tgctgcggaa cccgccgatg atcgagaaac cgagtgggac cacgatcacc agcagcaccg
      781 agatggcgat cgcatacaga aggaatcgcc cgaggtcgaa cccacgggca tgtggggtgg
      841 aggcgtttga ggcagtcatt tcagttggtc agcgctccct ccaggtcgcg gcggaggacg
      901 aacctctggt agagcaaggc gatcaccagt gacagcccaa agatgatcac cgacaccgcg
      961 ctggcgtacc cgaactgggt gcggcggaat ccccagtcca ccagataggt agccatcgtc
     1021 gccgtggaat tgacgggtcc tcccttggtt gtgacccaca ccaggtcgaa cagttgcaac
     1081 gcgccgatga tcgaaagaaa gatggagacc cggatggttg ggccgagcag ggggagggtc
     1141 acgaaccgga atccctgcca ccaggtggcg ccgtcgatcg ccgccgcctc ctcgagctcg
     1201 tcggggatgg tctgcaggcc ggccatgatc agcaccatgt ggaatccgaa gtacttccac
     1261 gagatgacga agaagaggct gtagagggca atgctggggt cggccagcca ctccttcgtg
     1321 aggtcacccg ccccggccga ggtcagcatc acgtcgaaca ggcccgcagg ccggaagatc
     1381 tgacgccata cgactcctgt gaccacctcc gagaggatga acggagcgaa gtacatggtc
     1441 ctgaacaggc cgcggccctt caggcttcgg ctcagcagca cggccagtgc tatggcgaac
     1501 ggtatctgga gggccaacga gagaccgatg atgatgaagt tgtgctggat cgcctcgatg
     1561 aagagcgggt cggcgaatgc ctctctgaaa ttgtccagac cgacccactc ggtcagcggc
     1621 tccaggccat tccagtcgta gccgctatag cggatgctct gcgtgatcgg gtagagaacg
     1681 aacatcccgt agagaaggag ggcggggagc aggaagccga tcaccatcgg ccagggcgcg
     1741 acaccgggcc ggcgatgcga acgccggagc gggcctgaag ggtcggcggt ttcggagttg
     1801 ctcgtcatcg gctcgatagt gggtggaggg ccgccgaaat ggcggccctc cacgtttttg
     1861 ctcacactac ggccgaggtc agctcgcggc gtcagtgatc gcctgagtca cctcttctgg
     1921 agaggcggtc cctgcgaaca gggtggctac agcgtcgttc accgccgcgc caagctcggg
     1981 cgagaagtac tggtcgagat agagctggac gaatgtcgcc ttgctcagtc cttcgagtac
     2041 cgctagctgg tacggatccg ttatcgagtc ctcggctccc ttggtcacgg ggagcgtggt
     2101 gccggtcgca ccgacgatcc gtgcgttgtc gacgttggtg atgaactcga ggaactggat
     2161 cgcctccggc tcggcgtcct tgccgacagc aaagccgtcg ccaccaccga acccgtcggt
     2221 tccggcaccg gctccgccat cgactgctgg gaatgggaac catccgaact cggcgggtag
     2281 acggtcggcg attggatcct ccgggacacc aattcccgac tggttggcgt aggcgcccgg
     2341 gccccactgg cccatgaggc tcatcgccgc ggcacctgtg ccgatggttc ccgactcacc
     2401 gtcgggaccg tcccacggtg cggc
//
LOCUS       DBNHXU010000354         2909 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2319246, whole genome shotgun sequence.
ACCESSION   DBNHXU010000354 DBNHXU010000000
VERSION     DBNHXU010000354.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2909)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2909
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2319246"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..591
                     /gene="ftsY"
                     /locus_tag="AC161X_08715"
     CDS             <1..591
                     /gene="ftsY"
                     /locus_tag="AC161X_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007266815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0006614 - SRP-dependent cotranslational
                     protein targeting to membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HIR4989674.1"
                     /translation="VGVNGTGKTTTIAKIAARLKDEGKKVILAAADTFRAAAGAQLQT
                     WGDRLGVDVVGGQEGGDPASVAYDAISSARAKGADVVIIDTAGRLHAKKNLMAEMSKI
                     YRVAAGDQGTLDEVLLVLDATAGQNGIAQVREFSEAVPVSGLVLTKLDGTARGGIVIA
                     IERELGVPVKLVGVGEGLRDMVPFDPDSFVKDLMED"
     gene            591..980
                     /gene="cdd"
                     /locus_tag="AC161X_08720"
     CDS             591..980
                     /gene="cdd"
                     /locus_tag="AC161X_08720"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002852416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HIR4989675.1"
                     /translation="MNTDDLAAAARAAAEGSYSPYSGFRVGAVVVGDRGEVFIGANVE
                     NGAYPASSCAEATAINTAVSHGVRKIETVAVACIDALDVEGAYPCGRCRQIMSEFDVS
                     TVIIAGGPGSEIREHTLDELLPHRFRL"
     gene            complement(983..1399)
                     /locus_tag="AC161X_08725"
     CDS             complement(983..1399)
                     /locus_tag="AC161X_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015620629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3052 domain-containing protein"
                     /protein_id="HIR4989676.1"
                     /translation="MAGYSGTPLPQKLGIKNGLTVVILDAPDSFMPTLGRLPSDVEVH
                     HEVTASNIFILFGSWADKLEEAFHRAKSLLPPDGAIWVAWPKRSSGVETDITEDTLRD
                     LFLPTGMVDNKVCAIDETWSGLRFVVRKENRATWPG"
     gene            complement(1403..1924)
                     /locus_tag="AC161X_08730"
     CDS             complement(1403..1924)
                     /locus_tag="AC161X_08730"
                     /inference="COORDINATES: protein motif:HMM:NF013412.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR4989677.1"
                     /translation="MSEIPPTYRTPHGLDSDLRQQILARSNAVLATINDDGSPHLTEL
                     LFYLDEKDRVLLPTPETTRKVKNVIARPTVTVFISVASGWASCTGTARLITGEEASRL
                     NQLNRERVLTPAGMATMGLLLAAYEDSTIEVTPARWLSWNDDRVLDTIVDLGGDIEAH
                     PPETWWTDLSAGS"
     gene            complement(1995..>2909)
                     /locus_tag="AC161X_08735"
     CDS             complement(1995..>2909)
                     /locus_tag="AC161X_08735"
                     /inference="COORDINATES: protein motif:HMM:NF013964.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HIR4989678.1"
                     /translation="LSEWVSARLDVSHETGKALVRTMRRIEDRPGLAVALADGEATFD
                     RVEALSRIQKDVGLLQHLDVAGVRRTAALKALITPEEEFRTADERFLVIQPSLDESWW
                     RLWGGLDGASGALVDKVLTEAADQLPELPDGTRGDASWRKATALVELCISDDPPPAQV
                     TVFVDAAEAATSNGQTGVVLEAGPRVGANALAAILCDATTEVVARTEDGRLMEYGRRL
                     RVAPPSLRRAVLLRDGNVCIADACESRYRIEAHHIIPWTEGGPTDPENLISLCWFHHH
                     VVVHERGFQPFRHPDHGRIRFRPPDRPR"
BASE COUNT          514 a          860 c          997 g          538 t
ORIGIN      
        1 gtcggagtca atggcacggg caagaccacc accatcgcca agatcgcggc ccggctcaag
       61 gatgaaggca agaaggtgat cctcgctgcg gccgacacct tccgtgctgc ggccggagcc
      121 caactccaaa cctggggcga ccggttgggt gttgacgtcg tagggggaca ggagggaggc
      181 gacccggcgt cggtcgccta cgacgccatc agctccgccc gtgccaaggg agccgacgtg
      241 gtgatcatcg acaccgccgg ccgtctgcac gccaagaaga acctcatggc tgagatgagc
      301 aagatctacc gagtggcggc aggggaccag ggcacgctcg acgaggtgct gttggtgctc
      361 gacgcgactg ccggccagaa cgggatcgcc caggtgaggg agttttccga ggcagttccc
      421 gtgagtggcc tcgtcctcac caagctcgac gggaccgccc ggggcgggat tgtgatcgcc
      481 atcgagcgcg aactgggtgt gcctgtgaag ctggttgggg taggggaggg gctccgagat
      541 atggtcccct tcgatccgga tagcttcgta aaagacctga tggaggattg atgaacacag
      601 acgatctcgc agctgccgcc cgtgccgcgg ccgaaggctc ctactccccg tactccgggt
      661 ttcgagtcgg cgcggtggtg gtcggcgatc gcggcgaggt gttcatcgga gccaatgtcg
      721 aaaacggcgc atatccggct tcgtcgtgtg cggaggcgac tgcgattaac acggcggtca
      781 gccacggcgt gcggaagatc gaaacggtgg ctgtggcatg catcgatgct ctggatgtgg
      841 agggtgccta tccctgtggc cgatgccgcc agatcatgtc cgagttcgac gtgtcaacgg
      901 tgatcatcgc cggcgggcct ggttcggaaa tccgggagca cacgctcgac gagctgctcc
      961 cgcatcgctt ccgcctgtga agtcatccgg gccaggtggc ccggttctct ttgcggacca
     1021 cgaacctcag ccctgaccag gtttcgtcga tcgcgcacac cttgttgtcc accatccctg
     1081 tcggcaggaa gaggtcgcgc aaggtgtcct cggtgatatc ggtctcgact cccgagcttc
     1141 gcttgggcca tgccacccag atcgccccgt cgggcggaag cagggatttg gcccgatgga
     1201 aggcttcttc gagcttgtcg gcccaacttc cgaagaggat gaagatgttg gacgcggtca
     1261 cctcgtggtg gacttccacg tcagagggaa gacgtcccag ggtcggcatg aaactgtcgg
     1321 gggcgtcgag gatcacgacg gtcaacccat tcttgatccc cagcttctgg ggaagtggcg
     1381 tgccgctgta tccggccaat gctcagctcc cggcgctgag gtcggtccac cacgtctccg
     1441 gcggatgggc ctcgatgtcc ccgccaaggt caacgatggt gtcgaggacc cggtcgtcat
     1501 tccaggagag ccaccgagcg ggagtgacct cgatcgtgct gtcctcgtag gcagcgagga
     1561 gcaggcccat ggtggccatc ccggccgggg tcagcacccg ttcccggttc agctggttga
     1621 gccgggaggc ctcctccccg gtgatgaggc gggcggtgcc agtgcaggat gcccagccgg
     1681 acgccaccga tatgaagacg gtgacggtgg gccgggcgat gacgttcttg actttcctcg
     1741 tggtctccgg tgtgggaaga agtactcgat ccttctcgtc gagatagaag aggagctcgg
     1801 tgagatgcgg cgagccgtcg tcattgatcg tggcgagaac ggcgttgctc ctggcgagaa
     1861 tctgttggcg caggtcagag tcgagaccgt gcggtgtccg ataggtgggt gggatctcgc
     1921 tcatggtcga gaaccctagg tgttgacggt gtgtctgact tcagccggcg ccggggtgtg
     1981 gggaagctcc acccctatct aggtcgatct ggtggccgga agcggatcct tccatggtcc
     2041 gggtgtcgga agggttggaa tcctcgttcg tggaccacca cgtgatgatg gaaccagcag
     2101 agcgagatca ggttttcagg atcggtaggc ccgccctcag tccaggggat gatgtgatgc
     2161 gcctcgattc gataccggga ttcgcaggcg tcggcgatgc agacattgcc gtcccgaagc
     2221 aacaccgccc gacgcaacga cgggggagcg actcgcagtc gtcgtccgta ctccatcagt
     2281 cgtccgtcct cggtgcgagc aacgacctcg gtggttgcgt cgcagaggat ggcggccagg
     2341 gcattcgcgc cgacccgagg cccggcctcg agtaccactc cggtttggcc attgctggtg
     2401 gcagcttcag cagcgtcgac gaacactgtg acttgagctg ggggcggatc gtcagaaatg
     2461 cagagctcga ccagggcggt ggccttgcgc cacgatgcgt cacctctggt tccgtcagga
     2521 agctccggga gttgatcggc ggcttcggtg aggaccttgt caaccagagc gcccgaggca
     2581 ccgtcgaggc caccccagag tctccaccag gattcatcga gagacggttg gatcacgagg
     2641 aagcgctcgt cggcggtccg aaactcttcc tcgggcgtga tcagcgcctt caaggcggcc
     2701 gtccgacgga ccccggcaac atcgagatgt tggaggaggc ccacatcttt ttggatccgg
     2761 gagagggcct cgacgcgatc gaaggtggct tctccgtcgg ccagagccac cgcgaggcct
     2821 ggtcggtcct cgatacgtcg catggtccgg accagagcct tacctgtttc atggctgaca
     2881 tcgagtcgcg cagacaccca ctccgagag
//
LOCUS       DBNHXU010000355         3809 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2913781, whole genome shotgun sequence.
ACCESSION   DBNHXU010000355 DBNHXU010000000
VERSION     DBNHXU010000355.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3809)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3809)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3809
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2913781"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..644
                     /locus_tag="AC161X_08740"
     CDS             <1..644
                     /locus_tag="AC161X_08740"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR4989679.1"
                     /translation="TLPDDLAPEALTDARTLMDHEQHRTLEYPPHTVFVESLAPPQEL
                     IIVGAVHIGQALASFAAQMGYRVTVIDSRGAFATPDRFPNARIIVGWPENVIDQMPLD
                     RRTWVVILSHAQPHEDPVLAAALKSQVRYIGTMGSRRTQDLRVGRLREMGFSDDDIAR
                     VRGPVGLDIGAESPQEVAVSILAEMTLVRYGHGTGVSLHGVEGRVHKQRPEDG"
     gene            682..3390
                     /gene="ppc"
                     /locus_tag="AC161X_08745"
     CDS             682..3390
                     /gene="ppc"
                     /locus_tag="AC161X_08745"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004953426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HIR4989680.1"
                     /translation="MKAISDEQLRADIRRLGNQLGDALVRQHGPELLDLVETVRALGK
                     SVRRGGSAEATQELDQLLSQLEVGDVIALVRAFTTYFYLANVTEQVHRVDQLATDDRY
                     LKSTVDRILEADVDEQELIEVLSRLEVRPVFTAHPTEAARRSILSKTRQLADLIEQRL
                     GVQDDDTRSMIDRRSAELIDQIWQTDELRLQRPSPVEEARSALYYLVQVADEVMPALA
                     DHVAFQLERLGPPVTAPIRFGSWVGGDRDGNPEVTPETTLETLILQHDRGLRHLISLV
                     ESLADELSMSQRLSPISEGLEISLAEDREHLPEIWQRENRRTAAEPYRLKCSYIHARL
                     INTNLRIQRGLPYRAGHDYADPSGLLADLEIMDGSLRASRGQLIADGALARVRRAAMT
                     FGFQMATLDVREHAAKHRSALSEVYSLVDTDYHGLDAVGRQGLLGSELAGSRPLTGPN
                     TVIGEEARNTIGTFHAIAEAKARYGDQVIESYVVSMTETPADVLEVAVLAREAGLIDL
                     RRGVASIGIVPLVETIDDLANAGPLLEGLFADPHYRRVVALRGDSQEVMLGYSDSNKV
                     GGITTSQWEIYKAQRVMRNIAADHGVHLRLFHGRGGTVGRGGGPTHAAILAQPYGTVD
                     GDLKVTEQGEVISEKYGHPDIAARNLELLLASVLESSLLHRRSRRPKEALDRWTEAMD
                     CFSSEAHTAYRELVEHPSLVPYFLTSTPVEELGRLNLGSRPSRRPGGVGGVEDLRAIP
                     WVFGWTQTRQIVPGWYGVGSGLQAARKAGYGEVIDEMAEEWSYFQTFLSNVEMTLAKT
                     DLDIAERYVDALVPSEHKGLLDRLRAEAELTSGEILSARNEEALIQDLPLLRRTLAVR
                     DLYLDPINYLQVSLLARSRAGETDPDLGRALLLTVNGIATGMRNTG"
     gene            complement(3394..>3809)
                     /locus_tag="AC161X_08750"
     CDS             complement(3394..>3809)
                     /locus_tag="AC161X_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989681.1"
                     /translation="GTRLRRMNLLLLGLAVVTTACSSPADEELVISSIPNCAEQEVIQ
                     AELSDEPGYATPEEAAEEVRQAIIDGGGRPDGKPVPELVELSYLTFYLPVEETPAEDG
                     DGLIVVMANPGGGFLANRAMFCTGTIPQPFPWGLE"
BASE COUNT          717 a         1253 c         1175 g          664 t
ORIGIN      
        1 gcaccctccc cgacgacctc gcccccgagg ctctcacgga tgccaggaca ctcatggatc
       61 acgagcagca tcggaccctg gaataccccc cacacacggt cttcgtcgag tcgctggccc
      121 cgccacagga gctgatcatc gtgggggcag tccacatcgg ccaggcattg gccagctttg
      181 ccgctcagat gggttatcgg gtgacggtca tcgactcccg gggtgcgttc gccacaccgg
      241 accgcttccc gaacgcgcgg atcatcgttg gatggccgga gaatgtgatc gatcaaatgc
      301 ccctcgaccg gcggacctgg gttgtgatcc tctcccatgc ccagcctcat gaggaccccg
      361 tcctggcggc agcactgaaa agccaggtca gatacatcgg aaccatggga agccggcgca
      421 ctcaggacct gcgcgtgggg aggctgcggg agatggggtt ctcagacgac gacatcgccc
      481 gggtgcgtgg accggttggc ctcgacatcg gcgccgagag cccacaagag gtggcggtct
      541 cgatcctcgc cgagatgaca cttgtccgct acgggcacgg gaccggggtg tcacttcacg
      601 gtgtggaagg cagggtgcac aagcagcgcc cggaggacgg ctgaatagcc gacccggttc
      661 gagcaccgat accctgccgg catgaaggcg atctccgacg agcaactgcg cgccgacatc
      721 cgtcggctcg gcaaccaact cggcgacgcc ctggtccgcc aacacgggcc agagctcctg
      781 gatttggtcg agacggtgcg tgcgttgggc aaatcggtgc gacgcggggg tagcgccgaa
      841 gccacacagg agctcgacca actcctttcg cagctggaag tcggagacgt cattgcctta
      901 gtccgcgctt tcaccaccta cttctacctc gccaacgtca ccgaacaggt gcatcgggtc
      961 gaccaactgg ccacagatga tcgctatctg aagtccacag tcgatcggat cctcgaggcc
     1021 gatgtggacg agcaggagct gattgaggtt ctctcccggc tcgaggtgag accggtgttc
     1081 acagcccatc ccaccgaggc agcccggcgc tcaatcctca gcaagacgcg ccagctcgcc
     1141 gacctgatcg agcaaagact gggagtgcag gacgacgaca cccggtcgat gatcgatcgt
     1201 cgctctgcgg agttgatcga ccagatctgg cagaccgatg agttgcggtt gcaacggccg
     1261 agcccggtcg aggaggcccg atctgccctc tattacctgg ttcaggtcgc cgacgaggtg
     1321 atgcccgcac tcgcggacca tgtcgccttc caactggagc ggttgggccc accggtcacc
     1381 gccccgatcc gcttcggctc gtgggtgggc ggcgatcgtg acgggaatcc ggaggtgacc
     1441 ccggaaacca cactggagac actgattctg cagcacgacc gcgggctgag gcacctgatc
     1501 tctttggtgg agtccctggc tgacgaattg tcgatgtccc agaggctgtc cccgatcagc
     1561 gagggcttgg agatttcgct cgctgaggac cgtgagcatc ttccggagat ctggcagagg
     1621 gagaaccgcc gcactgccgc cgagccctac cggctcaagt gctcctacat ccacgcccga
     1681 ctgatcaaca ccaacctcag gatccaacgg ggccttccct accgagccgg tcacgactac
     1741 gccgatccct ccgggctgct cgccgacctc gagatcatgg acggctcgct tcgggcatct
     1801 cgtggtcagt tgatagcgga tggggccctg gcccgggttc gtcgtgccgc catgaccttt
     1861 ggctttcaga tggccacgct ggacgtcagg gagcacgctg ccaaacaccg ttccgctctc
     1921 tcggaggtct acagcctcgt agacaccgac taccacgggc tggacgccgt tggccggcag
     1981 ggccttctcg ggtcggaact cgcaggatcg cggcccctca ccggccccaa caccgtcatc
     2041 ggggaagaag ctaggaacac gatcggcact ttccatgcca tcgccgaagc caaagcccga
     2101 tacggagacc aggtcatcga gagctatgtg gtctcgatga cagagacccc cgcggacgtc
     2161 ctcgaagtgg cggtgcttgc ccgggaggcg ggcctgatcg atctacgtcg tggagtggcg
     2221 tcgattggca tcgtcccgct ggtcgagacc atcgacgacc tggccaatgc cgggccactc
     2281 ctcgaaggcc tattcgccga ccctcactac cggcgggtgg ttgcgcttcg cggcgatagc
     2341 caggaggtga tgctcggcta ttccgactcc aacaaggtgg gcgggatcac caccagccag
     2401 tgggagatct ataaggcgca acgggtgatg cgcaacattg ccgccgacca tggggtacac
     2461 ctacggctct tccatggccg tggcggtacg gtcggaaggg gtggtggccc cacgcacgcc
     2521 gcgatcctcg cccagcccta cgggacggtg gacggcgacc tcaaggtcac agagcagggc
     2581 gaggtgattt cggagaagta cggccacccc gacattgccg cccgcaacct ggagttgctg
     2641 ctcgcatcgg tcctcgaatc atctctcctc catcgccgat ctcgacgtcc caaggaggcc
     2701 ctcgaccgat ggaccgaggc gatggactgc ttctcaagcg aggcccatac cgcctatcgc
     2761 gagttggtcg agcacccgag tctggtcccc tactttctga cctcgactcc ggtggaggaa
     2821 ttgggacggc tcaacctggg aagccggcca tcgcgtcgac cgggaggagt aggcggggtt
     2881 gaggatctgc gggcgattcc ctgggttttt ggatggactc agacccgaca gatcgtcccc
     2941 ggttggtacg gggtgggtag cggcctccag gctgcgcgca aggcgggtta cggcgaggtg
     3001 atcgacgaga tggccgagga gtggagctat ttccagacat tcctctccaa cgtcgagatg
     3061 accctggcca agaccgacct cgacatcgcc gagcgctacg tcgacgcatt ggtcccttcc
     3121 gagcacaagg gcctcctcga tcgtctccgc gcggaggccg agttgacctc cggagagatt
     3181 ctcagcgccc gcaatgagga agcattgatc caggatcttc ccctgctccg gcgaaccctc
     3241 gccgtccgcg acctctacct ggacccgatc aactatctcc aggtctcact gctggctcga
     3301 agccgggccg gggagaccga ccccgacctg ggccgcgccc tcctgctcac cgtcaacggg
     3361 atcgccaccg ggatgcgcaa cacaggctga gtgctattcg agcccccaag gaaacggctg
     3421 tgggatggtt cccgtgcaga acatggcgcg attggcgaga aatcctccac ccggattcgc
     3481 catcaccacg atcaaaccgt caccgtcctc ggccggagtc tcctcaactg gcagatagaa
     3541 agtcagatag ctgagttcga ccagttcggg aaccggtttc ccatccggtc tcccccctcc
     3601 atcgatgatt gcctgccgta cctcctcggc tgcctcttcg ggcgtcgcgt aaccgggttc
     3661 gtccgaaagc tctgcctgaa tgacctcttg ctcggcacaa ttcggaatcg acgagatcac
     3721 caactcctca tcggcggggg aactgcaggc cgtagtcaca acggccaggc ccagcagaag
     3781 gagattcata cgcctcaatc gagtccctc
//
LOCUS       DBNHXU010000356         2414 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2715275, whole genome shotgun sequence.
ACCESSION   DBNHXU010000356 DBNHXU010000000
VERSION     DBNHXU010000356.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2414)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2414
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2715275"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..357
                     /locus_tag="AC161X_08755"
     CDS             <1..357
                     /locus_tag="AC161X_08755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989682.1"
                     /translation="WLSVDDNGLTLVNVDGSRRVIPTGASSLLDATFSPDGTLIAGLT
                     GEGLTVFSLDEGTEVAVTSERPGMPQVVWSSDSRYVLYPGLRGVVVLDTSDGTVRELM
                     PTDIVTGLGLFDFDGQ"
     gene            366..923
                     /locus_tag="AC161X_08760"
     CDS             366..923
                     /locus_tag="AC161X_08760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014377388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR4989683.1"
                     /translation="MATFHVLAAGYGGERVASTVSLIVSGKSLVVVDPGMVASRSVIL
                     DPIAQLGYRPDDITDVVISHHHPDHTINIALFPNARVHDHWAIYDGDAWDDRPAEGFE
                     VAPDVTLWETPGHTAQDITTMVRAGDELVAFTHLWWYETAPFHDPLCTHPDDLHVHRE
                     RVLDVATLIVPGHGEPFVPDASTSR"
     gene            934..1662
                     /locus_tag="AC161X_08765"
     CDS             934..1662
                     /locus_tag="AC161X_08765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017592171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA deacylase
                     activity [Evidence IEA];
                     GO_function: GO:0002196 - Ser-tRNA(Ala) deacylase activity
                     [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HIR4989684.1"
                     /translation="MAGTGPIEELAATDSYLARAEGRVLEVLEGGLFLDRTVFYARGG
                     GQPGDVGLIRWDGGEVEVIDTIRREGKPFHVVAEGAVLPDPGTPVEGVIEWDRRYLTM
                     KTHTALHALSGLIFRDFGAKVTGGNMDPGVARMDFELDGISVEFGQTVERKLNEELTK
                     GYPTEVVFMARSEALKDPDLIRTKVNLIPEWVEEIRVIDIVGLDRQADGGTHVASTLE
                     VGEVRVVKTESKGKANKRMRIEIG"
     gene            1708..>2414
                     /locus_tag="AC161X_08770"
     CDS             1708..>2414
                     /locus_tag="AC161X_08770"
                     /inference="COORDINATES: protein motif:HMM:NF014704.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HIR4989685.1"
                     /translation="MLDFVLGLVLAGLLIRGWTRGFVREVLDLVGLILGVWIAFRLSA
                     PLGEFLSDRFGVSPEVAAIGAGIVLFVLFGVAMGVAARYLSKVMNLPGLTMINRVGGA
                     AVALGWGIVILLVVLNLALVLPLPPSWDQAIEESTVAQAIAGPEALPQQAFETVASSQ
                     VLSSLTALQSVLGASRAVPEGNEIIIIPGALEDEIRQVRDEVADLMVRINEYRAGQGL
                     NALTTSAVFTDVAETRAA"
BASE COUNT          404 a          731 c          787 g          492 t
ORIGIN      
        1 tggctttctg tcgatgacaa cggtctgacc ctggtcaatg tggacggatc gcgtcgcgtg
       61 atccccaccg gtgcgagctc gttgctcgat gccacctttt cccccgacgg caccctcatc
      121 gcgggcctca ccggggaagg gttgaccgtc ttctcgctgg acgagggaac cgaagttgcc
      181 gtcacctcgg agcgacctgg aatgcctcag gttgtgtggt cttccgacag ccgttatgtg
      241 ctctatcccg ggcttcgcgg cgtggtcgtt ctcgatacca gcgatgggac ggttcgtgag
      301 ttgatgccga ctgacattgt cactgggctc ggacttttcg acttcgacgg gcaatgatgt
      361 aggccatggc cacctttcat gtcctggcgg cggggtatgg cggcgaacgg gtcgcctcca
      421 cggtgtcgct catcgtctct gggaaatcgc tggtcgtggt cgatcccggg atggtggcct
      481 cgcgttcggt gatcctcgac ccgatcgccc agttgggcta tcggcccgat gacatcaccg
      541 acgttgtgat atcccaccac catcccgatc acacgatcaa catcgcgttg tttccaaatg
      601 cccgggttca tgatcactgg gcgatctacg acggcgacgc ctgggacgat cgtccggcgg
      661 agggcttcga agtcgcaccc gatgtgacgc tctgggagac cccggggcat accgctcagg
      721 acataacaac gatggtgcgg gccggtgatg agctggtggc cttcacccac ctctggtggt
      781 acgagaccgc tcccttccac gatccgcttt gcacccaccc cgacgacctc cacgtccacc
      841 gggagcgggt gctcgatgtc gccacactga tcgtgcccgg ccacggtgag cccttcgtgc
      901 ctgacgcctc gacctctcgg tagcctccgc aatatggccg gaaccggacc gatcgaagaa
      961 cttgccgcaa ccgattcata tctggcgcga gccgagggtc gggttctcga ggttctggag
     1021 ggcgggctct tcctcgaccg gacggtcttc tatgcccgtg gtggaggtca acccggcgat
     1081 gtcggcctga tccgctggga cggcggcgag gtcgaggtaa tcgacaccat ccggcgagag
     1141 ggcaagccgt tccatgtggt ggctgagggc gccgtgctcc ccgatccagg gacaccggtg
     1201 gagggtgtca tcgaatggga tcggcggtat ctgacgatga agacccacac cgcacttcac
     1261 gcgctgtccg ggctgatctt tcgtgacttc ggggccaagg tgactggagg gaacatggac
     1321 ccgggagtgg ccaggatgga cttcgagctc gacgggatct cggtcgagtt cggccaaacc
     1381 gtggagcgga agttgaacga agaactgacc aagggatatc cgaccgaggt cgtgttcatg
     1441 gcccgcagcg aggccctgaa ggaccccgac ctgatccgga ccaaggtgaa cctgatcccg
     1501 gagtgggtcg aggagattcg tgtcatcgac atcgtcgggc tcgatcgcca ggcagacggt
     1561 ggcacccacg tcgcttccac cctcgaggtg ggtgaggtgc gggtggtcaa gaccgaatcc
     1621 aagggcaagg cgaacaagcg aatgaggatt gagatcggct gagcgcatta cgcagaacgc
     1681 gatgcgtaat gcgtaagact cagatcaatg ctcgattttg tgctcggtct ggttcttgcc
     1741 gggcttctga ttagaggttg gacccgggga ttcgtccgtg aggtcctcga tcttgttggg
     1801 ctcatcctcg gggtctggat tgcctttcgg ctgagcgccc ccctcggcga gttcctctcc
     1861 gatcggttcg gggtctcccc ggaggtcgcc gccattgggg cgggcatcgt gctcttcgtc
     1921 ttgttcgggg tggccatggg agttgctgcg cgctacctgt ccaaggtgat gaacctgccc
     1981 ggtctcacca tgatcaaccg ggtcggtggc gccgcggtgg ctctgggttg gggcatcgtc
     2041 atccttctgg tcgtcctcaa tctggccctg gtgttgccgc ttcctcccag ttgggatcag
     2101 gccatcgagg aatccaccgt ggcgcaggca atcgccgggc ccgaagcatt gccccaacag
     2161 gctttcgaaa cggtcgcctc gagtcaggtg ttgtcctcgt tgaccgccct gcagtcggtt
     2221 ctcggggcga gccgggcggt acccgagggg aatgagatca tcatcatccc gggcgccctc
     2281 gaggacgaga tcagacaggt ccgtgacgag gttgccgacc tgatggtccg aatcaacgag
     2341 taccgcgcgg gccagggtct gaacgcactc accaccagcg ccgtgttcac cgatgtcgcc
     2401 gagacccgag ccgc
//
LOCUS       DBNHXU010000357        12664 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4106911, whole genome shotgun sequence.
ACCESSION   DBNHXU010000357 DBNHXU010000000
VERSION     DBNHXU010000357.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 12664)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 12664)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12664
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4106911"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1823
                     /locus_tag="AC161X_08775"
     CDS             <1..1823
                     /locus_tag="AC161X_08775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042649.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HIR4989686.1"
                     /translation="VVSLEMINQRLIPVAIEPRSVLAEYNPGYSCFTVHSSTQVPHAL
                     GGAISKTFGMKSTSVRVIAPEVGGGFGSKLNVYNDEILVAFAAKQLGQPVKWTETRRE
                     AAGSTIHGRGWIGTSTLVGTKDGQFLGYKLDAIADMGAYTQNFTVAIPLLGLWIASGQ
                     YKMPVSWTVACVVTNTMTTDAYRGAGRPEAIYYLERIIDAYAHEIGMDPLEVRKKNFW
                     QPDEFPAQMGCGFSLDSGNYEAAVDKLVEVSDYAGLRRRQEEARAEGRLVGVGIGSYV
                     EVCGFGPAILAEIGFSWTNYGLPASFNGSGLVRVNPDATVTVVIGTGPTGQGHETTWA
                     QLVGDGLGIPVEDISVLHGDTREHPMGIGTFGSRSISVDGTATYEATLKVKEKAARIA
                     AHLLEASADDIVFADGGAHVAGTPDKRVEWAAIAKAAYQAHTSPDDVDSGLEAHTSFS
                     PGNATWPFGTHLAMVEVDPDTGDVEILEYHAVDDCGNVISPMIVNGQVHGGIAQGIGQ
                     AMFEDAIYDADGNMLTGSLLDYPLPTAADLPSFDLHRTVTPSPVNPLGVKGIGEAGTI
                     GSAHTMVNAVVDALTPMGVKHIDMPLRPKRVWQAMQEARG"
     gene            1825..2685
                     /locus_tag="AC161X_08780"
     CDS             1825..2685
                     /locus_tag="AC161X_08780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013134.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HIR4989687.1"
                     /translation="MYPRSFEYVAPASLEEAFSALGDDSKVMAGGMSLLPLMKMRLFS
                     PGTVVDIGRIMGLDTVEDAGDHISIGALVRHATTASDPLVAANAAALATAASLTGDVQ
                     VRNRGTTCGSLAHADVAADQPAAAIACRATMVAQSASGTREIGSESFCVDALTSDLRP
                     DEILVAIKIPKAGPGEGSAYDKLGRRGGHSDYAVAGAAAWVKKSNGTVEDARVALTGV
                     GTRAMLAEGVGQAMIGTDGSEAAIKSAAEHALEGVTVLEDLYGSVEYKSHLAKVFVSR
                     ALKAALANAS"
     gene            complement(2747..3601)
                     /locus_tag="AC161X_08785"
     CDS             complement(2747..3601)
                     /locus_tag="AC161X_08785"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR4989688.1"
                     /translation="MRPVVAGVDLTAMGRRVADRARMMAEASGVDLNLVHVLEPLGEA
                     MIEPGLARLMRDHQTTEAGRLAEWVRERSEVPVTLEVVKGSPSWELAARGKNASLVVV
                     GSSSIDAFAVGPVAKRVARKTTADTLVVRRQPRVPYRRIIAAVDFSEQSRIAVNRSLE
                     AFPGADVTVLFSLPSRFDPILSAAGLYREELEASRGARLAIAEERMDEFTATWDGDIR
                     TLVVDGPPTETIEEIVRRRGADLVVVASRGASATRMVLLGTIAEGLVTEAPCDVLVVR
                     SKNLFRRP"
     gene            complement(3631..4311)
                     /gene="aat"
                     /locus_tag="AC161X_08790"
     CDS             complement(3631..4311)
                     /gene="aat"
                     /locus_tag="AC161X_08790"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019877722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HIR4989689.1"
                     /translation="MAIEPIPSQFLLPEPSDDDGDVIAVGGDLAPGTLLQAYRKGMFP
                     MTLPDGHLGWWSPVERAIIPLRGLHISRSLRQSCRRYRVSVDADFEGVIYSCADPTRR
                     EDGWITDEFVNAYVELHRLGWAHSIEVWDDDGALAGGLYGVAIGGMFAGESMFHDARD
                     ASKVALVHLVVVMSQGDGRLLDVQWQTPHLQTLGATVIGRRAYFGLLPDALEGPDPFA
                     SSHQVDSL"
     gene            4361..5503
                     /locus_tag="AC161X_08795"
     CDS             4361..5503
                     /locus_tag="AC161X_08795"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012568831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR4989690.1"
                     /translation="MPDYVVIGGGIAGVAAAARLAPHGQVTILEMESTLAFHTTGRSA
                     AMFTASYGGHASRPLARASRAFLENPPDESADSPLLARRGALWIGNHDQAPLLADRAE
                     EGRRLGANLEMLDARQAAELVPVLNRERLAGGLLDPEAADLDVAALHQAFVRLARRAA
                     TTIITSSPVRAIERTVTGWRVRAEATTLECDAVVNAAGAWGDQVAALAGILPVGLTPM
                     RRTAFMVPGDDSYARWPLVDDVEDGFYFKSDGTQILCSLAEENPDMPGDPRPRPEDVA
                     MAIERINLATDLAIRTVSSEWVGLRTFAPDREMVIGEEPDAPGFFWLVGQGGTGIMTS
                     PAYGELVASQVLRIPLPPSLTAAGVDPVTTLPNRFRESPLIESGTE"
     gene            5500..7749
                     /locus_tag="AC161X_08800"
     CDS             5500..7749
                     /locus_tag="AC161X_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002686266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FdhF/YdeP family oxidoreductase"
                     /protein_id="HIR4989691.1"
                     /translation="MRQVNAGGGFPAIWYTWRKAREAGGVRSLYKAMRSNNACKTCAL
                     GMGGQLGGMVNEKGHFPEVCKKSIQAMSADMQGAIQPSFFKRFDLEQLRAFTPREMEA
                     AGRLTQPLYAGPIDRRYRVISWDEALMRVEKKMVSTEPDQAFFYTSGRSSNEAGFLLQ
                     LLARIYGTNNVNNCSYYCHQASGVALSAVTGSGTATVELGDLDSMGKDDIVFIIGANP
                     ASNHPRLMTTLAHLKRRGGRVVVVNPLRETGLVRFKVPSDVRSLFFGSKIADEYVQPH
                     IGGDIAFLAGVSKRIVEMGAVDPRFVEDHAEGWEEFRASLELLDWDTIVSRSGVPKSA
                     IDRVADLYAAAPRAVFCWAMGITHHEHGVQNVQAIANLAMTRGMLGRTCCGLLPLRGH
                     SNVQGIGSMGVSPKMKDSVLQALETEFDVKLPTTAGMDTMASVVRASEGGIRFALCLG
                     GNLFGSNPDSEFATRAMGAIDMVVYMSTTLNTGHAWGRGKETIILPVLARDEEPQPTT
                     QESMFNYVRLSDGGPIRHEGPRAEVSVIADLGQAVTEDRILDWDSMRRYGRIREAIAK
                     VIPGYEAIGNIEQTKKEFQITGRTFHDREFPTESGRARFHVLELPVPAGDNGSFRLMT
                     VRSEGQFNTVVYEDYDLYRGQDRRDVIMMSQEDIDRLGFQPDQQVTVTSDTGEMHGLL
                     VRPIDIRPGNTVMYYPEANVLVPRGVDPASKTPGFKNTVVRVTPSRNSVVQGSYPDLS
                     HAITTDRTT"
     gene            complement(7746..8741)
                     /locus_tag="AC161X_08805"
     CDS             complement(7746..8741)
                     /locus_tag="AC161X_08805"
                     /inference="COORDINATES: protein motif:HMM:NF018028.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HIR4989692.1"
                     /translation="MTQPDQEPIVDPDIDSEEVPDTDRRGLFTRMARGASERVMDVVD
                     VDMVLDHVDINALLERVDINAVLERVDVDALMARVDVRALVDRAGIPEIVAESTGHLT
                     GSALDLFRKPIVGIDEIVFHGLNRVIGRDTNEFPQGPGSLTAWVDEKEEEKAQIIGGP
                     PRGTGAVRTGRYAGPVTRLLAVMLDVFLITTGFTLVVGVISFLGDLLFSDTVTIPEGG
                     LWYRIGLGVFAFLYVWLSLAVFGKTAGKSVLGLRVVGADGTVGLGGNRALIRTLTFPI
                     SIAVFGLGLIGVIWGRERRAWHDHFAGSAVVYDWGSRAAQMSSPLADWLERRGGS"
     gene            complement(8781..10448)
                     /gene="ilvD"
                     /locus_tag="AC161X_08810"
     CDS             complement(8781..10448)
                     /gene="ilvD"
                     /locus_tag="AC161X_08810"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019047263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004160 - dihydroxy-acid dehydratase
                     activity [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009082 - branched-chain amino acid
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HIR4989693.1"
                     /translation="MPTKIRSQEVTEGPAKAGARAMLRAVGLTEEDFDKAQVGVVSAG
                     NEVTPCNLTGPELSRHAKAGVAETGGVGLIFSTIAVSDGISQGHEGMRASLVSREVIA
                     DSVELVMHAERFDALVAIAGCDKSLPGMLMAAARLDLPAIFLYGGSSLPGHLNGRDIS
                     IVDVFEGIGSESEGKITPEELLAIERAACPGAGSCAGMFTANTMAAVGEALGMSLPGS
                     ASVPAIDPRLADYAKESGEAVMRLLEADLRPRQIMTRPAFENAVTTVMALGGSTNAVL
                     HLMAIANEAGVAFDLEDFDRISRSTPHIGDLKPFGRYHMVDLDRIGGVPVVLKALLEE
                     GLLHGDVLTVTGSTMAENLADTTFPEGQDVIAPPSAPLAPQGGIAVLRGNLAPEGAVV
                     KVAGIDFEVFEGPARVFDGEQSALESLFNHQIQHGDVVVIRTEGPKGGPGMREMLSIT
                     AAIKGAGLGRDVMLITDGRFSGGTTGLCIGHVAPESEVGGPIGLVEEGDRVKVDIPGR
                     RLDVLVDDETLAERAANWSPHPVRYATGALAKYAKLVGSAGSGAVTG"
     gene            10510..11520
                     /gene="ilvC"
                     /locus_tag="AC161X_08815"
     CDS             10510..11520
                     /gene="ilvC"
                     /locus_tag="AC161X_08815"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015262097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HIR4989694.1"
                     /translation="MATIYYDSDSDLGLIQGKKVAVIGYGSQGHAHALNLKESGVDVV
                     VGLRSGSQRVAEAETAGLTVMDPAEAAAWANVVMMLVPDQVMGTIYRESVASHLESGD
                     TLLFAHGFNIHFGEIVPPENVDVAMVAPKGPGHLVRRTYEQGSGTPCLLAVFQDASGG
                     AHDLALSYASAIGGGRAGILETTFKEETETDLFGEQVILCGGVSELIKASFETLVEAG
                     YQPESAYFETLHELKLIVDLLYEGGLSWMRYSVSDTAEYGDYTRGRRIITDETRQAMR
                     DTLAEIQSGAFAHEWMAQAREGAPFLYEQRALNQAHQIEQVGKELRTMMPFMEPKTAP
                     ES"
     gene            complement(11517..12533)
                     /locus_tag="AC161X_08820"
     CDS             complement(11517..12533)
                     /locus_tag="AC161X_08820"
                     /inference="COORDINATES: protein motif:HMM:NF013702.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HIR4989695.1"
                     /translation="MPPRWLRRILIDPAVTLLVVLAALSLPIWAIGAAFVSRYVPGKW
                     RILRLLWFLFLYLGVEALAMIMMFGLWIGSGFGWKIRSPAFETAHYWLLGLMLRLIIG
                     SAKRTFKLTIDFESAPRVTADDVDRRPILVLSRHAGPGDSMLLIDALANGYLRRPRIV
                     LKDFLQWDPAVDVILNRLPTSFVHTGKNIGDPVVEAIAALARTMSPDDAFVIFPEGGN
                     FSEGRRDRAIKKLIEIGRPDLAERARDLKNTLPPKPKGVMTALSTAPQNSDVIFVGHS
                     GLESFITARDIWRGMPMDTSVAVRVWQYPAETIPPPEGQETWLYDIWDEIDNWIEARM
                     EKTG"
BASE COUNT         2454 a         4112 c         3952 g         2146 t
ORIGIN      
        1 tggtcgtctc cttggagatg atcaaccagc gcctgatccc ggtggccatc gagccgcggt
       61 cagtgctggc cgagtacaac cccggctaca gctgcttcac ggttcacagc tccacccagg
      121 tccctcacgc ccttggaggc gcgatttcaa agaccttcgg gatgaagtcc acctcagttc
      181 gggtgatcgc acctgaagtg ggcggtggtt tcggcagcaa gctcaacgtg tacaacgacg
      241 agatcctcgt ggcgttcgcc gccaaacagt tgggacaacc ggtgaagtgg accgagaccc
      301 gtcgcgaggc ggcagggtcg accatccacg gccggggctg gattggcacc tcaaccctgg
      361 tcggaaccaa ggatggccag ttcctcgggt acaaactcga cgccatcgcc gacatgggtg
      421 cctacaccca gaacttcacg gtcgccatcc cgttactcgg gctctggatc gcatccgggc
      481 aatacaagat gccggtctcg tggaccgtgg cctgtgtggt caccaacacc atgaccaccg
      541 atgcctatcg gggcgccggc cgtccggaag cgatctatta cctggaacgg atcatcgacg
      601 cctacgcaca tgagatcggg atggacccgc tcgaagtgcg gaagaagaac ttctggcaac
      661 cggacgagtt cccggctcag atgggctgcg gattctctct cgactccgga aactatgaag
      721 ccgccgtcga caagctggtc gaagtcagcg actacgccgg gctccgtcgc cgtcaggagg
      781 aggcaagagc cgagggtcgt ctcgttggag tcgggatcgg ctcctatgtg gaggtgtgtg
      841 gtttcggccc ggcgattctc gctgagatcg ggttctcctg gaccaactac gggcttccgg
      901 cctcgttcaa cggatcgggc ctggtccggg tcaaccctga cgccacggtc accgtggtca
      961 ttgggaccgg ccccaccggc cagggtcacg agaccacatg ggcccaactc gtcggagacg
     1021 gcctcggtat cccggtcgag gacatcagcg tcctccacgg cgacacccgg gagcatccga
     1081 tggggatcgg caccttcggc agccgctcga tatcagtcga cgggacggcc acctacgaag
     1141 cgaccttgaa ggtcaaggag aaggcggcca ggatcgcggc acatttgctg gaggcatccg
     1201 ccgacgacat cgtgttcgcc gacggaggcg cccacgtcgc gggcacgccc gacaagcggg
     1261 tcgagtgggc cgcgatcgcg aaggccgcct atcaggcaca caccagcccc gatgacgtcg
     1321 acagcgggct cgaggcccat acctcgttta gcccgggcaa cgccacctgg ccgttcggta
     1381 cccacctcgc aatggtcgaa gtcgaccccg acaccggcga cgtcgagatc ctcgagtatc
     1441 acgccgtgga tgactgcggc aacgtgatca gcccgatgat cgtcaacggc caggttcacg
     1501 gcggaatcgc ccaggggatc ggccaggcga tgttcgagga tgcgatctac gacgccgacg
     1561 gcaacatgct caccggctcg cttctcgact acccactccc gactgcagcg gatctcccct
     1621 cgttcgatct ccatcggacg gtgaccccgt caccggtcaa cccgctgggc gtcaagggga
     1681 tcggcgaggc tggaaccatc ggctcggctc acaccatggt caatgccgtg gtggacgcgc
     1741 tgacgccgat gggggtcaaa cacatagaca tgcccttgcg gccgaagcgg gtgtggcagg
     1801 cgatgcagga agcgaggggc tgagatgtat ccgagaagct tcgaatatgt ggccccggcc
     1861 agcctcgagg aggcgttctc cgccctggga gacgactcca aggtgatggc tggcgggatg
     1921 agcctgcttc ccctgatgaa gatgcggctg ttctcaccgg gaacggtggt ggacatcggc
     1981 cggatcatgg gtctcgacac cgtggaggat gcgggagatc acatctcgat cggcgccctg
     2041 gtgcgccacg ccaccacggc atccgacccg ctggtggcgg cgaacgccgc cgccctggcc
     2101 actgcagcat cactcaccgg tgacgttcag gtgaggaacc ggggtaccac ctgtggctcc
     2161 ttggcgcacg cagacgtagc tgccgaccag ccggcggccg ccatcgcctg ccgggcgacg
     2221 atggtcgccc aatcggcttc ggggaccaga gaaatcggct cggagagctt ctgcgtcgat
     2281 gccctcacct cggacctgcg gcccgacgag atcctggttg caatcaagat cccaaaggcg
     2341 gggcctggcg aagggtcggc ctacgacaag ctgggtcggc gcggcgggca ctcggattac
     2401 gcagtggccg gagctgcagc ctgggtcaag aagtcgaacg ggaccgttga ggatgccagg
     2461 gtggctttga ccggggtcgg gaccagggcc atgctcgccg agggcgtggg ccaggcgatg
     2521 atcggaaccg atgggtcgga agcggcgatc aaatcggccg cagagcatgc actcgaaggg
     2581 gtgacggtgc tcgaagacct ctacggctcg gtcgagtaca agtcacacct ggccaaggtc
     2641 tttgtgagtc gggcgctcaa agcggcactg gcaaacgcga gctgacactc atcctaaatg
     2701 cagggcgtca cgcatctgtg acgccctgca tgaagaagaa agcgcgtcag ggccggcgaa
     2761 acaggttctt ggaccggacg accagcacgt cgcaaggcgc ctctgtgacc aggccttcgg
     2821 cgatggtccc cagcagaacc atcctcgttg cagaggcgcc ccggctggcc acgactacga
     2881 ggtccgcccc ccgccgcctc acgatctctt cgatggtctc ggtcggaggg ccatcgacca
     2941 ccagggttcg gatgtccccg tcccaggtag ccgtgaactc atccatccgc tcctcagcaa
     3001 tcgccagcct ggcccctctc gaggcttcca actcttcgcg ataaaggccg gcggcggaga
     3061 ggatcgggtc gaatcgggaa ggcagggaaa agagcaccgt gacgtcggcg cctggaaaag
     3121 cctcgaggga gcgattcacg gcaatacgtg actgttcgga gaagtcgact gcggcgatga
     3181 tcctccgata agggacccgg ggctggcgac gaaccaccag ggtatccgcc gtggtctttc
     3241 gtgcgacccg cttcgctacc gggcctacgg caaaggcgtc gatcgaggaa gatccgacca
     3301 cgacgagact cgcgttcttg ccacgagcgg cgagctccca ggagggtgac cctttgacca
     3361 cttccaaggt gaccggcacc tcgctccgct ccctgaccca ctcggcgagg cgcccggcct
     3421 cagtcgtttg gtgatcgcgc atcagtcggg ccagcccggg ctcgatcatc gcttcgccaa
     3481 gaggctcgag cacatgcacc agattgaggt ccacgccgct tgcctcggcc atcatgcggg
     3541 cccgatcggc tacacgccgg cccattgctg tcaggtcaac tcccgctacg acgggccgca
     3601 aagaactcct cccggggaag tgggacgact ctacagactg tccacctggt gcgacgaagc
     3661 gaagggatca gggccctcga gagcgtcggg gagcagcccg aagtaggcgc gccgcccgat
     3721 cacagtcgcc ccgagcgtct gaagatgggg tgtctgccac tggacgtcga gcagcctgcc
     3781 gtcgccctgg ctcatcacca ccaccagatg gacgagagcg accttcgaag catcacgagc
     3841 gtcgtggaac atcgactcac cggcgaacat gcctccaata gcgaccccgt agaggccgcc
     3901 cgccagggca ccatcgtcat cccatacctc gatggagtgc gcccaaccga ggcggtggag
     3961 ctcgacgtat gcattgacga actcgtcggt gatccacccg tcctcccgtc gggtcggatc
     4021 ggcgcagctg tagatcacac cttcgaaatc ggcatccacc gacacccggt agcgacgaca
     4081 cgactgacgg agcgaccggc tgatgtggag gccacgcagc gggatgatgg ccctctccac
     4141 cggggaccac cacccgaggt gcccgtcagg aagagtcatc gggaacatcc ctttgcgata
     4201 ggcctgaagc agggtgccgg gagcgaggtc cccgcctact gcgatcacgt cgccgtcgtc
     4261 atcactcggc tctggaagca agaactggga ggggatgggt tctatggcca accaactgat
     4321 tgttccagag tgcagcgtgc ggtgtccgta ggctgcccag atgcctgact atgtcgtcat
     4381 aggcggaggg atcgccggtg ttgccgccgc ggctcgcctc gcaccgcacg gtcaggtcac
     4441 gatcctcgag atggagtcaa ctctcgcctt tcacaccacc ggccgctcgg cggcgatgtt
     4501 caccgccagc tacggcgggc atgcgtcccg gccactggcc agggccagtc gggcgtttct
     4561 cgagaatccg cccgatgagt ctgccgattc gccccttctc gcccgccgtg gcgcgctatg
     4621 gatcggcaac cacgaccagg ccccactgct ggcagaccgg gccgaagaag ggcggaggct
     4681 gggcgccaat ctcgagatgc tcgatgcccg ccaggccgct gaattggttc cggtgctgaa
     4741 tcgagagcgc ctcgccggtg gactactcga cccggaggcc gccgacctcg acgtagctgc
     4801 cctccatcag gctttcgtgc gcctggcgcg ccgcgcagcc accaccatca tcacctcgag
     4861 cccggtcagg gcgatcgaac gaaccgtaac cggctggcgg gttcgagccg aggccacgac
     4921 cctggagtgc gatgcggtcg tgaacgctgc aggagcctgg ggggatcaag ttgcggctct
     4981 tgccggaatc ctcccggttg gcctgacccc gatgcggcgt accgcattca tggtccccgg
     5041 cgatgactcc tatgcccgct ggccgcttgt cgacgatgtc gaggacggct tctatttcaa
     5101 gtctgacgga acacagatcc tgtgctcttt ggctgaggag aatccggaca tgcctggcga
     5161 cccccgcccc cgccccgagg acgtggcgat ggccatcgaa aggatcaatc tggccaccga
     5221 cctcgcgatc cgcacggtga gctcggagtg ggtcggactc cgcactttcg caccagatcg
     5281 agaaatggtg atcggcgaag agcccgacgc gcccggcttc ttctggctgg tgggtcaggg
     5341 cgggaccggc atcatgacct ctcctgccta cggcgagcta gtggctagcc aggtgctgcg
     5401 gattccgcta ccgccgtcgc tgaccgctgc cggagtcgac ccggtgacga cccttcccaa
     5461 ccggttccgg gagagccccc tcatagaatc cggcacagaa tgagacaggt caacgcgggc
     5521 ggcggttttc ccgcgatctg gtacacctgg cgcaaggcca gggaggcagg cggagtccgc
     5581 agtctctaca aggccatgcg gtcgaacaac gcctgcaaga cgtgcgcttt ggggatgggc
     5641 ggccaactcg gtgggatggt caacgagaag ggccacttcc cggaggtctg caagaagtcg
     5701 atccaggcga tgagcgcaga catgcagggt gcgattcagc ccagcttctt caaacgcttc
     5761 gacctcgaac aattgcgagc gttcacccca cgggaaatgg aggcggccgg gcgtctcacc
     5821 cagcccctgt atgcggggcc aatcgatcgt cgctacaggg tgatctcctg ggatgaggcc
     5881 ctgatgaggg ttgaaaagaa gatggtttcc accgagccag atcaggcctt cttctacacc
     5941 agtgggcggt catccaacga ggcaggtttc ttgctccagt tactggcgcg gatctacggg
     6001 accaacaacg tcaacaactg ctcgtactac tgccatcagg catcgggcgt ggctctgagc
     6061 gcggtcacag gctcgggcac cgccactgtc gaattgggtg atctcgactc gatgggcaag
     6121 gacgacatcg tcttcatcat cggagccaac cccgcctcta atcatccccg actgatgaca
     6181 actctcgccc atctcaaacg ccgcgggggc cgggtcgtgg tggtcaaccc gctgcgcgag
     6241 acgggtctgg tccggttcaa ggtcccatcg gatgtcagga gcctcttctt cggcagcaag
     6301 atcgccgacg agtacgtcca gccccatatc ggaggcgaca tcgccttcct cgcaggagtt
     6361 tccaagcgga tcgtggagat gggggccgtg gacccacgat tcgtagaaga tcacgccgaa
     6421 ggatgggagg agtttcgggc ttctcttgaa ctgctcgact gggacaccat cgtcagccgc
     6481 tccggtgtgc ccaagtcagc aatcgaccgg gtcgctgatc tgtacgcggc cgccccgcgg
     6541 gcagttttct gctgggcgat ggggatcacc caccacgagc acggggtgca aaacgttcag
     6601 gcgatcgcca acctcgccat gacccggggc atgctgggac gcacctgctg tggcctgctc
     6661 ccgctgcgcg gccactccaa cgtccagggg atcggctcga tgggggtcag cccgaagatg
     6721 aaggacagcg ttcttcaggc tttggagacc gaattcgacg taaagcttcc cactacagcc
     6781 ggtatggaca ccatggcctc ggtggtccgc gcctcggaag gcggtattcg cttcgcgttg
     6841 tgtctcgggg gaaacctctt cggctccaac cccgactcgg agttcgccac tcgagcgatg
     6901 ggtgcaatcg acatggttgt ctacatgtcg acgacgctga acaccggcca tgcctggggc
     6961 cggggcaagg aaacgatcat cctccccgtc ctggctcgtg acgaagagcc gcagcccaca
     7021 acccaggagt cgatgttcaa ctatgtgcgg ctatccgacg gcggtcccat acgccatgaa
     7081 gggccacgtg cggaggtttc ggtgatcgcc gacctcggcc aagcggtgac cgaggatcgc
     7141 atcctcgatt gggactcgat gcgccgatac gggcggattc gcgaggcgat cgccaaggtg
     7201 atcccggggt atgaggcgat tggcaacatc gagcaaacca agaaggaatt ccaaatcacg
     7261 gggcgcactt tccacgaccg cgagttccct acggaatcgg gacgcgcccg gttccacgtg
     7321 ctcgagctgc cggtgcccgc cggtgacaac ggcagcttcc gactgatgac tgtccgcagc
     7381 gaaggtcagt tcaacacggt tgtgtacgag gactatgacc tgtatcgagg tcaggatcgc
     7441 cgggatgtga tcatgatgag ccaggaagac atcgaccggc tcgggttcca acccgatcag
     7501 caggtaacag tcaccagtga caccggggag atgcacgggt tgctggtgcg cccgatcgat
     7561 attcgcccgg ggaacaccgt catgtactac ccggaggcca acgtcctggt cccccgaggg
     7621 gtggacccag cctcgaagac gccggggttc aagaacacgg tggtgagggt gactccctca
     7681 cgtaattcgg ttgtacaggg gtcatatccg gacctgtcgc acgcaattac gacggaccga
     7741 acgacctagc tgccgccccg acgctccagc cagtcggcca gagggctcga catttgcgca
     7801 gcccgactcc cccagtcgta gaccacggcc gagccggcaa agtgatcgtg ccaggcgcga
     7861 cgctccctgc cccagatcac accgatcagg cccaaaccga agaccgcgat gctgatggga
     7921 aaggtcaatg tccggatcag ggcgcgattg ccgccaagac ctaccgtccc gtccgctccg
     7981 acaaccctga gaccgaggac cgacttgcca gctgttttcc cgaacacagc cagggacaac
     8041 cacacataga ggaaggcgaa tacacccagg cctatgcggt accagagccc tccctcgggg
     8101 atggtgaccg tgtcgctgaa tagaaggtcc cccagaaagg agatcacccc cacgaccagg
     8161 gtgaagccag ttgtgatcag gaagacgtcg agcatgaccg ccaacagccg ggtgaccggg
     8221 cccgcgtagc gaccggtccg caccgcaccg gtgcctcgag gaggcccgcc aatgatctgc
     8281 gccttttcct cctccttctc atcgacccac gccgtcaggc tccccggacc ctgcgggaac
     8341 tcattcgtat cacgaccgat cactcgattc agcccatgga agacgatctc gtcgatccca
     8401 acaatcggct tgcgaaacag gtccagggca gacccggtga ggtggccggt gctctcggcc
     8461 acgatctcgg gaatccccgc ccgatcaacg agggccctga cgtcgactct ggccatcagg
     8521 gcatcgacgt cgacccgctc cagcactgcg ttgatgtcga cccgttccag cagcgcattg
     8581 atatctacgt ggtcgagaac catgtccacg tccacgacgt ccatgacccg ctcgctggcg
     8641 ccgcgtgcca tacgggtgaa aagaccgcgg cgatcggtgt ccggaacttc ctccgaatcg
     8701 atgtcgggat cgacgattgg ctcttggtct ggttgggtca cgactgaaac gctagcccgc
     8761 cggcacctgc ctggggtagg tcaaccggtc acggcgccgg atccggccga gccgaccaat
     8821 ttggcgtatt tggccaacgc cccggtcgcg taccggacag gatgcggtga ccagttggcc
     8881 gcgcgctcgg caagggtctc gtcatcaacc aggacatcga gcctgcgacc gggaatatcc
     8941 accttgaccc ggtctccttc ctcgaccagc ccgatcggcc cacccacctc ggactcggga
     9001 gccacatgac cgatgcacag acccgtggtc ccgccagaga aacgcccgtc ggtgatcaac
     9061 atgacgtctc tgccgaggcc cgcgcccttg atggccgcgg tgatcgaaag catctctcgc
     9121 atgccaggcc cacctttggg cccttcggtg cgaatcacga ccacatctcc atgctgaatc
     9181 tgatgattga acagtgactc gagagcgctt tgctcgccgt cgaagacccg agccggcccc
     9241 tcgaacactt cgaagtcgat gccggccacc ttcaccaccg ctccttccgg cgcgagattc
     9301 ccgcggagaa cggcgatccc accctggggg gcgaggggcg ccgaaggcgg ggcaatcacg
     9361 tcctggcctt cgggaaaggt ggtgtcggcc agattctcag ccatcgtcga tccggtgacc
     9421 gtcagcacgt caccgtgcag gagaccttcc tcgagcaaag ctttcaggac caccggcact
     9481 ccaccaatgc ggtcgagatc gaccatgtgg tagcggccaa agggcttgag atcgccgatg
     9541 tggggcgtcg acctcgaaat ccgatcgaaa tcttcgaggt cgaaggcgac gccggcctcg
     9601 ttggcgatcg ccatcaggtg gagaacggca ttggtcgagc caccaagggc catcactgtg
     9661 gtgaccgcgt tctcgaacgc cgggcgggtc atgatctgac ggggcctgag atctgcctcg
     9721 agcagcctca tgaccgcctc acctgactcc ttggcatagt cggcgaggcg agggtcgatc
     9781 gccggaaccg acgccgagcc ggggagcgac atgccgagcg cctcccccac cgcagccatg
     9841 gtgttggccg tgaacatccc ggcacatgag ccggcgccgg gacaggctgc ccgctcgatg
     9901 gcgaggagct cttcgggagt gatcttgccc tctgactccg agccgatccc ctcgaacacg
     9961 tcgacgatgg agatgtcccg accgttcaag tgccccggca ggctcgaacc tccatagaga
    10021 aagatggccg gaaggtcgag ccgcgccgcg gccatcaaca tccccggcaa ggacttgtcg
    10081 cacccggcga tggcgaccag ggcgtcgaag cgctcggcat gcatcacaag ctcgaccgag
    10141 tcggcgatca cctctctgga taccagggag gcccgcattc cctcgtggcc ctgtgatatc
    10201 ccgtcggaga cggcgatggt cgaaaagatg aggccgacac cacctgtctc agccactccg
    10261 gccttggcat gacgggagag ctcaggtcct gtgagattgc agggcgtcac ctcgttgccc
    10321 gccgaaacca ccccgacctg ggccttatcg aagtcctctt cggtgagccc gactgcacgc
    10381 aacatggcgc gggctcctgc tttggccggt ccttcggtca cttcctggga tctgatcttt
    10441 gttggcatct cccgacgaat cctaaggagt cgggtttggt cgcggaccga aaccgaccac
    10501 aattcgctca tggcaacgat ctactacgac tccgacagcg atctaggcct gatccagggc
    10561 aagaaagtgg ccgtcatcgg ctacggaagc cagggtcacg ctcacgccct caacctcaag
    10621 gaatccggag tcgacgtggt agtcggcctc cggtccggat cgcagcgggt tgcggaggcc
    10681 gaaaccgccg gcctgaccgt tatggacccc gccgaggcgg ctgcttgggc caacgtcgtg
    10741 atgatgctcg tccccgacca ggtcatgggg accatctacc gcgaatcggt cgcctctcac
    10801 ctcgaatcag gagacacact cctcttcgct cacgggttca acatccattt tggggagatc
    10861 gtgccccccg agaacgtcga cgtcgccatg gtggcgccca agggcccggg ccacctggtg
    10921 cgacgcacct acgaacaggg atccggcact ccctgcctcc tcgccgtgtt tcaagacgcg
    10981 tcgggcggag cccacgatct cgctctctcc tacgcctctg ccatcggcgg cgggagggcc
    11041 gggattctcg aaaccacttt caaagaggag accgagaccg acctgttcgg tgagcaggtg
    11101 atcctttgtg gaggcgtgtc ggagctgatc aaggcctcat tcgagacctt ggttgaggcg
    11161 ggctatcagc cggagtcggc ttacttcgag acgcttcacg agctcaagct gatcgtcgac
    11221 ctcctctatg agggaggcct ctcctggatg cgctattcgg tttccgacac cgcggagtac
    11281 ggcgactaca cccggggaag acggatcatc accgacgaga cacggcaggc catgagagac
    11341 accctcgccg agatccagtc aggtgccttc gcccacgaat ggatggctca ggcccgcgaa
    11401 ggcgctccct tcctctatga gcagcgagcc ctgaaccagg cgcatcagat cgagcaggtc
    11461 ggcaaagaat tgcggacgat gatgccattc atggagccca agacggcgcc ggagtcctag
    11521 cccgtcttct ccatcctcgc ctcgatccag ttgtcgatct cgtcccatat gtcatagagc
    11581 caggtctcct ggccttctgg aggcgggatc gtctccgccg ggtactgcca gacccggacc
    11641 gcaacggaag tatccatcgg cattccccgc catatgtcac gggcagtgat gaacgattcc
    11701 agacccgaat ggcccacgaa gatgacgtcg gagttctgtg gcgcagttga gagcgccgtc
    11761 atgacaccct ttggctttgg gggcagagtg ttcttcaggt ctcgagcccg ctcagccaga
    11821 tccggtcggc caatctcgat gagcttcttg attgcccgat ctcgccggcc ctcgctgaag
    11881 ttgcctccct cgggaaagat cacaaatgcg tcgtcgggcg acatggtccg cgccaacgcg
    11941 gctatcgcct cgaccaccgg gtcgccgatg ttcttaccgg tgtggacgaa ggaggtcggg
    12001 aggcgattca ggatcacatc caccgccgga tcccactgca ggaagtcctt gagcacgatc
    12061 cgcggacgtc tcaagtagcc gttggcgagg gcgtcgatga gcaacatcga gtcgccaggg
    12121 ccggcatgcc ggctgaggac aaggatcggc ctccgatcca catcatcggc ggtgacacgc
    12181 ggtgcgcttt cgaaatcaat cgtcagcttg aaagtccgct tggccgaacc aatgatcaac
    12241 cgaagcatca gaccgagcag ccagtagtgg gccgtctcga aggcggggga ccggatcttc
    12301 cacccgaatc cggacccgat ccacaagccg aacatcatga tcatggccag agcctcgacg
    12361 ccgaggtaca gaaagagaaa ccacagcagc cgcagtatcc gccatttccc ggggacgtag
    12421 cgggagacga atgccgctcc gatcgcccaa attggcagcg agagcgcagc gaggaccacc
    12481 agaagggtca ccgccggatc gatgagaatc ctgcgcagcc acctcggggg caacctcatc
    12541 cccggatgcc ggccagatac ccggccgatg cttcataggc gccactgatc aactcgtggg
    12601 tcgatgagac atcgcgccac tttcgctgac ggctgtcgtc gaaggggacg gcgttgcccg
    12661 aggg
//
LOCUS       DBNHXU010000358         2295 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2322821, whole genome shotgun sequence.
ACCESSION   DBNHXU010000358 DBNHXU010000000
VERSION     DBNHXU010000358.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2322821"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..822
                     /locus_tag="AC161X_08825"
     CDS             <1..822
                     /locus_tag="AC161X_08825"
                     /inference="COORDINATES: protein motif:HMM:NF040246.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACT domain-containing protein"
                     /protein_id="HIR4989696.1"
                     /translation="TTKAVDAGPSRDWLGFARTGRAKSKIKQWFTKERRDEALSDGKE
                     MIQALLRRDAGELSAADKEKFLESIAQELGHHDVESLQVGLGEGNVSIDTVSARLNRI
                     LTPDTGDDFFQPRRRRPLDDAQVVVEGQDDMLVHLARCCSPVPGDPIVGYVTVGRGVS
                     VHRADCTNVSSLNERRERTVEVAWPAGRTGAFVVWIQVEALDRTRLLKDVTTVISDTG
                     GNITASSTHSGADRVAILRYEVELSDPSQLPKLLTDLRGVDGVFSAFRLATDPVP"
     gene            826..1479
                     /locus_tag="AC161X_08830"
     CDS             826..1479
                     /locus_tag="AC161X_08830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017933613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR4989697.1"
                     /translation="MLIVRVPAWLADTNAYIVADEPGGMAVVIDAPPEPEKVGAVLAE
                     HGLAAVGILLTHGHIDHSGGSGSLSKTTGASVYVHPDDDFLTLHPAQQLSSMWGMNPP
                     GSYDLPERFESLADGQVLDLAGLSFEVRQTPGHTPGHCCFLLAEQETLFSGDQLFAGS
                     IGRTDFPYGDGAALFRSMREKVMTLDDEVRVLPGHGSETTIGRERATNPFRDNWERS"
     gene            complement(1509..1838)
                     /locus_tag="AC161X_08835"
     CDS             complement(1509..1838)
                     /locus_tag="AC161X_08835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989698.1"
                     /translation="MSQGRRRGEKAAVALAAGAAAWVIGATMIYRSGGLNTAGLLPSV
                     GCLIALWGALRSDVRLMWFGSGVVVVSAVALVFSVGLIVAPAGAALILGSLLLARSTR
                     LSHRGLA"
     gene            1982..>2295
                     /locus_tag="AC161X_08840"
     CDS             1982..>2295
                     /locus_tag="AC161X_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015255349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HIR4989699.1"
                     /translation="MSFQPPKGTDDIGAPQSSVWRSVLGDWEEWSARYGYPLVMTPIF
                     EATELFQRGVGDTSEVVTKQMYTFTDRGGRSVTLRPEGTAGVVRAFLDSGQKGAWKGA
                     YSG"
BASE COUNT          409 a          730 c          735 g          421 t
ORIGIN      
        1 accaccaagg ctgtcgacgc cggcccgagc cgggactggc tgggtttcgc ccgtaccggc
       61 cgtgccaagt cgaagatcaa gcagtggttc accaaggagc gccgggacga ggccctctcc
      121 gacggcaagg agatgatcca ggccctgctt cggcgagatg cgggggagct gagtgcggcc
      181 gacaaggaaa aattcctcga gtcgatcgcc caggagctcg gtcaccacga tgtcgagtcc
      241 ctgcaggtcg ggctgggaga gggcaatgtc tcgatcgaca ccgtgtcggc ccgcctcaac
      301 cggatcctga ctccggatac cggtgacgat ttcttccaac cgaggcgccg tcgcccattg
      361 gacgatgccc aggtggtggt cgagggccag gacgacatgc tggttcatct cgcccgttgc
      421 tgttctccgg ttcccggcga tccgatcgtg gggtacgtca cggtcggccg tggggtatcg
      481 gtccaccgtg ccgactgcac caacgtttct tccttgaacg agcgccggga gcggaccgtg
      541 gaggtggcct ggccggccgg gcgcaccggg gccttcgtgg tctggatcca ggtcgaagcc
      601 ctggaccgca cacggctcct caaggatgtc accaccgtca tcagcgacac cggtgggaac
      661 atcacagctt cgtctactca ctccggggca gaccgggtgg cgattctccg atacgaggtc
      721 gagttgtccg atccgagcca gttgcccaag ctgctcaccg acttgcgagg ggtggacggg
      781 gtcttctccg cctttcgcct ggccaccgat ccggttccgt agaccgtgct catcgtccgt
      841 gtgccggcgt ggctggccga caccaacgcc tacatcgtcg ccgacgaacc tggtggcatg
      901 gcggtggtga ttgacgctcc cccggagccg gagaaggttg gcgccgtgct cgccgaacac
      961 gggctcgcgg ccgtcgggat cctgcttact cacggccata tcgaccacag cggtggtagc
     1021 ggctctcttt ccaagaccac cggcgcctct gtctacgtcc atcctgacga cgactttctg
     1081 accttgcatc cggcacagca gttgtcctcg atgtggggga tgaatccgcc cggttcgtac
     1141 gacctaccag agcggttcga aagtcttgct gatggtcaag tcctcgacct cgccgggctc
     1201 agcttcgagg ttcgacagac accgggtcat accccggggc attgttgttt cctgcttgcc
     1261 gagcaggaaa cgctgttttc gggtgaccag ttgttcgccg ggtcgatcgg cagaaccgac
     1321 ttcccatatg gggacggggc agctctgttc cggtcgatgc gggagaaggt gatgactctc
     1381 gacgacgagg taagggtgct gcctgggcat ggttcagaga cgacgatcgg gcgagagaga
     1441 gcgaccaacc cgtttcggga taattgggag cgttcctgat tcctagattt cgagtctgga
     1501 accgggtctt aggcaagacc tcggtggctc agccgagtgg agcgggcgag tagtagcgac
     1561 cccaatatga gggcggcacc agcaggggcc acgatcagcc cgacgctgaa gacgagtgcc
     1621 acagctgaca cgacgacaac acctgatccg aaccacatca gcctcacgtc tgacctcaac
     1681 gcaccccaaa gagcgatgag gcatcccacg ctcggcaaca aaccggcggt gttcagacct
     1741 ccggaccggt agatcatcgt tgctccgatc acccaggcgg cggcgccggc cgcaagcgcc
     1801 acggccgcct tttcaccccg cctccgtccc tgagacatgt ccagacgctt ccttctcgag
     1861 agtgatccgt tgggcgggca aattctactg gcaatgcgcc aagtcccaca ctcgttgcga
     1921 ccggtgaccc gtcgcacttg ggtcgagggc ccaggtaccc gatgcctatc ctccccgtct
     1981 gatgagcttc caaccgccca agggcaccga cgatatcggc gcgccgcagt cgtcggtctg
     2041 gcggtcggtt ctcggggatt gggaggaatg gtcagcccgc tacggctacc ccctggtgat
     2101 gaccccgatc ttcgaggcga ctgagctatt ccaacgcggg gtgggtgaca ccagcgaggt
     2161 cgttaccaag cagatgtaca cgttcaccga ccgcggcggt cggtcggtca cgctccgacc
     2221 ggaggggacc gccggggtcg tgcgtgcctt tctcgactca ggacaaaaag gggcctggaa
     2281 gggcgcctat tccgg
//
LOCUS       DBNHXU010000359         3920 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2521169, whole genome shotgun sequence.
ACCESSION   DBNHXU010000359 DBNHXU010000000
VERSION     DBNHXU010000359.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3920)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3920
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2521169"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1475
                     /locus_tag="AC161X_08845"
     CDS             <1..1475
                     /locus_tag="AC161X_08845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003887725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HIR4989700.1"
                     /translation="QCQKAVMTNTFAELGLPEHLIAVLERRDITSPFPIQALTIPDVL
                     AGRDLCGRAPTGSGKTLAFGLPLVARIGRGKTRRPRGLILAPTRELAEQITAELRPLA
                     KASNRSMASVYGGVSYTPQRRQLHQGVDLLVACPGRLEDLINQGDVDLRDVDVVVVDE
                     ADRMADMGFLPAVRRLLEMTSPTRQTILFSATLDGDVAVLTQRYQRDPVRHESDVQED
                     AESDVEHVFWMIDRADRVAHTAEVVASASPSIIFTRTRHGADRLAKQLERSGVTAVAM
                     HGGRSQNQRNRALKDFTTGRAQALVATDVAARGIHVEGVASVIHFDPAEDDKAYLHRS
                     GRTARAGASGIVVSLVEGSQVRAAKRLQHSLGIGTQIPTRPAPTGHSMEPIREERKKR
                     PSAPTAPSHRPVSRDQSGSTLYVGNLPYSVTEDSLSAMFRDHGPVHSATILKYKDTDR
                     SRGFGFVTVGEGKGSAAIAEIDGREIAGRRLRVKAARPRG"
     gene            1511..2302
                     /locus_tag="AC161X_08850"
     CDS             1511..2302
                     /locus_tag="AC161X_08850"
                     /inference="COORDINATES: protein motif:HMM:NF014035.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HIR4989701.1"
                     /translation="MSTSAPLSVKAWKLALIGLTAGLLSGGFGIGGGIVMVPLLVAVG
                     LDRHRATATSLAAIVPIAIAGAITFSVSGEMNLELGIFIGVGGVVGSILGASLMNRMS
                     ARSLTVVFGVILLLAAVRMIAGGDPLPGSADFGQSAQIAIALGIGLISGFFAGVAGIG
                     GGVVIVPATVLLLGFGQHEAQGTSLVAIVLTAIAGTLVNLRNKRIRLEDGLAAGLAGV
                     VGSIVGARIALGVEGRTLSLVFGILVLILAVRTLYQAIRAPEVAI"
     gene            complement(2299..2526)
                     /locus_tag="AC161X_08855"
     CDS             complement(2299..2526)
                     /locus_tag="AC161X_08855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989702.1"
                     /translation="MKTHRFDAVSFIAGLAFAILGLVFLVPDTPQELIDALVNSGGWF
                     WPVALLIVGLAVIVPVLLPKKEEAIEAGDSD"
     gene            complement(2545..3762)
                     /locus_tag="AC161X_08860"
     CDS             complement(2545..3762)
                     /locus_tag="AC161X_08860"
                     /inference="COORDINATES: protein motif:HMM:NF015957.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HIR4989703.1"
                     /translation="MEQTNTTTEAPEPTVAGAEELIRPTEGRMLGGVAQGLANRFGIA
                     PWVVRAAFILTAFAGGVGVALYAAGWFLIRSEDEAESAAERVFSGATTSRSWVGIGLI
                     FLAALILLDNFTFLAGGVVWAVGLLIVGLLLYTGDLPRLINRSGEQAADKEGVQQMTT
                     TDTKVTTTEIPTPPIFPPSGGTVGGGVPPTPTPTPPILPPAEHKPREHSLLGRLTIGF
                     MLLGLGVLALLDNIPGVPIEPEPRHYMALAVTILGIGLLVGAFIGRARWLILLGVIMV
                     PTLLFSPALEYSWNSDDFERSVQPTSFAELDESYSLDVGNLLIDLRQLPWDGREVDLT
                     VSVDAGNVEIRLPDDVGLTGQAEVEVGRVAGPNMEASGLGNPALTFNDSGPDGTVNLD
                     ASVDIGNIEISQG"
BASE COUNT          675 a         1263 c         1232 g          750 t
ORIGIN      
        1 gccaatgcca gaaagcagtc atgaccaata cctttgccga gctcggccta cccgagcatc
       61 tgatcgcggt tctcgaacgc cgcgacatca catcaccatt tcccattcag gccctcacca
      121 tccccgacgt tctcgccggg cgtgatcttt gcggtcgtgc cccaaccggc tccgggaaga
      181 cactcgcctt cggcctgccc ctggtagccc gaatcggccg cgggaagacc cgccgaccac
      241 gcggtctcat ccttgccccc acccgggagc tggccgagca gatcacagcg gagcttcgcc
      301 ccttggccaa ggcatccaac cgctccatgg catcggtgta cggcggtgtc agctacaccc
      361 cgcagcgccg tcaactccat cagggcgtcg atctcctcgt tgcctgccct ggccggctcg
      421 aagacctgat caaccagggt gacgtcgacc ttcgtgatgt cgatgtcgtc gtcgtggatg
      481 aagccgaccg gatggccgac atgggcttcc tgcccgccgt gcgtcgcctc ttggagatga
      541 cctcgcccac caggcagacg attctcttct ctgcgaccct cgatggtgat gttgcggtgc
      601 tcacccagcg ttaccagcgc gacccggttc gccacgagtc cgacgtccaa gaggacgcgg
      661 agagcgatgt cgagcatgtc ttttggatga tcgatcgcgc cgatcgggtg gcacacaccg
      721 ccgaggtggt ggcctccgcc agcccttcga tcatcttcac caggacgcgg catggtgccg
      781 accgtctcgc caagcaattg gagcgatccg gggtcaccgc ggtcgccatg cacggcggaa
      841 gatctcagaa ccagcggaac cgggccttga aggacttcac gaccggtcgc gctcaagccc
      901 tggtcgccac cgatgtcgcg gcgagaggca tacacgttga aggggttgcc agtgtgatcc
      961 acttcgatcc tgcagaagac gacaaggcct atctccatcg gtcgggcagg accgctcgtg
     1021 ccggggcatc cggcatcgtc gtctcgctgg ttgaaggttc gcaggtgcgg gcagccaagc
     1081 gcctccagca ctcgcttggg atcggaaccc agattccgac ccgccccgct cccaccggac
     1141 attcgatgga gccaattcgc gaggagcgca agaagcgccc ttcggcacca actgctcctt
     1201 cacaccggcc ggtcagtcgg gatcagtccg gctcgacgct ctacgtcggc aatttgccct
     1261 actcggtcac cgaagacagc ctgtctgcga tgttccgtga ccatggtccg gtgcattccg
     1321 ccaccatcct caagtacaag gacacggatc gctcacgagg cttcggtttc gtgactgtcg
     1381 gggagggcaa aggctctgcg gccattgccg agatcgacgg ccgtgaaatc gccggccgcc
     1441 ggctacgggt caaggcggcg cgccctcgcg gttgacatgt tcggatccac tccccattta
     1501 gggtgagctg gtgtccacct cggcaccgct gtcggtaaag gcctggaagc tcgctctgat
     1561 cgggctcact gcgggtctgc tctcgggtgg tttcgggatc gggggcggga tcgtgatggt
     1621 cccgcttctc gtggccgtcg ggctcgaccg gcatcgggca accgccacct ctcttgcggc
     1681 catcgtcccg attgcgatcg ccggagctat caccttctcg gtatcgggtg agatgaacct
     1741 cgagcttggg atcttcatcg gcgtcggggg cgtagtcggc tcgatcctgg gggcgtccct
     1801 catgaaccgg atgagtgcgc gttcgttgac cgtcgtcttc ggagtgattc tgctgttggc
     1861 tgcggtgcga atgatcgccg gcggcgaccc gcttcccggc tccgccgact tcggtcagtc
     1921 ggcgcagatc gcgattgccc tcggaatcgg gctcatctcc ggcttctttg ccggggtcgc
     1981 cgggatcggt gggggggtgg tcatcgtccc cgcgaccgtc ctgttgctcg gattcggcca
     2041 acatgaggcg cagggcactt cactggtggc gatcgtcctc accgcgattg caggcacgct
     2101 cgtcaacctc agaaacaagc gaatccgcct cgaggacgga ctcgctgccg gcttagccgg
     2161 tgtggtcggt tcgattgtgg gggcccggat cgcactcgga gtggagggtc gcactctctc
     2221 gctggtgttc gggatcctgg tgctgatcct cgccgtgcgg accctctatc aggcgatccg
     2281 ggccccagag gtagctatct agtcggagtc accggcctcg atggcctcct ccttcttggg
     2341 aaggaggacc gggacgatca ccgccagacc aactatcaag agggcgacgg gccagaacca
     2401 gccgcccgag ttgacaaggg catcgatgag ttcttgtggc gtgtcgggga ccagaaagac
     2461 caacccgagg atcgcaaatg ccaatcctgc gatgaaggag actgcgtcga atctgtgtgt
     2521 cttcattttg atcaatcctc tcgatcagcc ttgtgagatt tcgatgttgc cgatgtcgac
     2581 gctggcatcg aggttgacgg ttccgtcggg tccggagtcg ttgaacgtca aggcagggtt
     2641 gcccaggccg gacgcctcca tgttgggccc tgccacccga ccgacctcga cctcggcttg
     2701 tccggtgaga ccgacatcgt cgggcagccg gatctcgaca ttcccggcat ccactgacac
     2761 cgtcaggtcg acctctcggc cgtcccaggg gagttgcctc agatcgatca ggagattgcc
     2821 gacatcgagg ctgtatgact catcgagctc ggcgaaactg gtcggttgca ccgaccgctc
     2881 gaaatcgtcg ctgttccagc tgtactcgag cgccggggag aagagcaagg tcgggaccat
     2941 gatcaccccg agcaggatca gccacctggc gcggccgatg aaggctccca ccaacaaccc
     3001 aatgccgagg attgtcaccg ccagcgccat gtagtggcgg ggctcaggct caataggcac
     3061 ccccggaatg ttgtccagca gagcgagaac gcccaggcca agcagcatga atccaatggt
     3121 gagccgtccc aggagcgagt gctcacgcgg tttgtgttcc gccgggggca ggatcggcgg
     3181 ggtaggcgtc ggagtcgggg ggacgcctcc gccgaccgtg cctcccgacg gggggaagat
     3241 gggtggggtt gggatctcgg tcgtggtgac cttggtgtct gtggttgtca tttgttgaac
     3301 tccctccttg tcagctgcct gctccccgga tctatttatc aggcgcggga gatcgcccgt
     3361 gtagaggaga aggcccacaa tcagcaggcc tacagcccat accacccctc cagcgaggaa
     3421 ggtgaagttg tcgagcagga tcagggcggc gaggaatatc agtccgatcc cgacccacga
     3481 tcgtgaggtc gtcgctccgc tgaacactcg ctcggctgca gactcagcct catcttcgga
     3541 gcggatgaga aaccatccgg cggcgtacag ggcgacgccg acaccgccag cgaacgccgt
     3601 gaggatgaag gcggcccgca ccacccatgg tgcgatcccg aatcgattgg ccaagccttg
     3661 ggccactccg ccgagcatcc gaccctcggt gggccgaatc agttcctccg caccggcgac
     3721 cgtgggctca ggggcctcgg tagttgtgtt ggtttgttcc atagacccca tatggccagg
     3781 attaacgatg cgcggcaatc ggggaccacc ctgagatgcc ccttgatcgc tcagggttct
     3841 ccccgattga ggttgggggc acggcccgcc atcatctccc tgtgagtcga cgtcgcgtct
     3901 tagagttcca ccgccgtcgg
//
LOCUS       DBNHXU010000360         3134 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3512878, whole genome shotgun sequence.
ACCESSION   DBNHXU010000360 DBNHXU010000000
VERSION     DBNHXU010000360.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3134)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3134
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3512878"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..146)
                     /locus_tag="AC161X_08865"
     CDS             complement(<1..146)
                     /locus_tag="AC161X_08865"
                     /inference="COORDINATES: protein motif:HMM:NF019604.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HIR4989704.1"
                     /translation="MARIAIVGDGPGGLSAGLFLAKNGHEVTIFGTDTTAMHYAELHN
                     YLGIP"
     gene            184..1098
                     /locus_tag="AC161X_08870"
     CDS             184..1098
                     /locus_tag="AC161X_08870"
                     /EC_number="3.2.2.-"
                     /EC_number="4.2.99.18"
                     /inference="COORDINATES: protein motif:HMM:NF012933.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032993 - protein-DNA complex [Evidence
                     IEA];
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase family protein"
                     /protein_id="HIR4989705.1"
                     /translation="MRGYARRRTIMPTVETVELPITGALDLRSTLRPLHGWFADDGWW
                     LTARTPEGPGTIRIRRTRDHLIGEAWGEGGEWLLASAGPIGGLNDDPTLFSTNHPLVA
                     DLHHRHPGMRFGRTGLVFDALVAAIVGQKVTGSEATWAMRGLRREFGDPAPGPRPGLS
                     LPPDPEKMAAAPYWALHELHLEKRRADALRRVSRDVTSIEGTASQSPALAAAVLEGHI
                     GVGPWTVAKTLMVSHGDPDQVEVGDFHVKHIVVSHMTGRPRGSDEEMLELLEPFRPQR
                     GRVVRLLHTLGHEPKFGPRMAPRDITTI"
     gene            complement(1099..2274)
                     /locus_tag="AC161X_08875"
     CDS             complement(1099..2274)
                     /locus_tag="AC161X_08875"
                     /inference="COORDINATES: protein motif:HMM:NF012348.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:1901704 - L-glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine synthetase family protein"
                     /protein_id="HIR4989706.1"
                     /translation="MALFSLQLNRVMIPHPGAHHESFPDVEARFSMDDVRPGWENGTG
                     VVVASITKDGAPVELAPRSILDRVVGEWEELGLTPMIGFELEAFLFEPDGDGGWRPIH
                     TPGSYVYGTGTAVDPTGTIQAIMETAHDMGLDLESVNSEYDDGQFELTLRYDKALQAA
                     DDAFLFKVMAREVAAKRGYLLSFLGKPLTERGGSGIHVNISLVDRDGKNAFSDPAARD
                     GLSELARQAIAGLVHDHVSMSALVAPTVNAYKRLKPGSLNGYYANWGYDHRGVAVRVP
                     PANESGTRLEHRLADGATSIHLAAAASLVAALRGVRLELECPPAESQDCITGADADMM
                     APPSLGAALDALESDKEFVEAFSPGYVEGFLAVKRAEWDRYMAHTTDWELEEYLPYY"
     gene            2471..>3134
                     /locus_tag="AC161X_08880"
     CDS             2471..>3134
                     /locus_tag="AC161X_08880"
                     /inference="COORDINATES: protein motif:HMM:NF012345.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HIR4989707.1"
                     /translation="MRALVIEHDPLSTPDRVGEHLERRGARLEPFVVVEDINHPEVTA
                     TFPDNGHDLVVLMGSPWSVYEERLQGWVGPELEFIRRQASSGVPILGICFGAQAMSAA
                     MGGKVTRASRPEYGWVSIESQVDEIAGGPWFQYHHDEFTLPPGAIELARNASGLQAFQ
                     SGKNLAVQFHPEMTAELIASWCEVDGGEEMVDVGIDPDKVIEESRLLEKTTQPSLERM
                     LDW"
BASE COUNT          531 a          981 c         1050 g          572 t
ORIGIN      
        1 ggtatcccga ggtagttgtg gagctcggcg tagtgcatcg cggtcgtgtc ggtcccaaag
       61 atcgtcacct cgtgcccgtt ttttgcgagg aacaggcctg cggatagtcc acctggcccg
      121 tcacccacga tcgcaatcct tgccattcac attcccttct gtctgaggcg tgaacctcga
      181 tggatgcgtg gctatgcccg tcggcggacg atcatgccaa ccgtggagac cgtggaactg
      241 cccatcaccg gggctctcga cctgcgaagc accttgaggc cgctccatgg gtggtttgcc
      301 gacgatgggt ggtggttgac ggcccgtacc ccggaggggc cgggcacgat tcggattcgc
      361 cgcacaaggg accatctcat cggggaggca tggggcgaag ggggcgagtg gcttctggct
      421 tcggcaggcc cgatcggcgg gctcaacgat gatccgaccc tcttcagcac caatcacccc
      481 ctggtggcgg acctccacca tcgccacccg gggatgcgat ttggcaggac cgggctggtg
      541 ttcgatgcgc tggtggcggc gatcgtcggt cagaaggtga ccggaagcga agccacgtgg
      601 gcgatgcgcg gcctgaggcg ggaatttggc gatccggcgc cggggccgcg acccggcctt
      661 tcactccctc cggatccgga gaagatggcg gcggcgcctt actgggctct tcacgagctg
      721 cacctggaga agcggcgagc cgacgccttg cgccgggtat cgcgtgacgt cacatcgatt
      781 gaggggacgg ccagccagtc tccggcactg gcggcagccg tcctcgaggg ccacatcggc
      841 gtgggcccat ggactgttgc caagaccctc atggtttccc acggcgaccc cgaccaggtg
      901 gaggttggcg acttccacgt caagcacata gtggtcagtc acatgaccgg tcgaccccgg
      961 ggcagcgacg aggagatgct ggagttgctc gagccgtttc ggccacagcg ggggagggtc
     1021 gtccggctcc tgcacaccct cggccacgag ccgaagttcg ggccacggat ggcgccccgc
     1081 gacatcacca cgatctgatc agtaatacgg gaggtactcc tcgagctccc aatcggtggt
     1141 gtgtgccatg tagcgatccc actccgcccg cttcacggcc aggaatccct ctacgtaacc
     1201 cggtgagaac gcctcgacga actccttgtc gctctccagt gcatccaagg ccgcccccag
     1261 gctgggtgga gccatcatgt cggcgtcggc acccgtgatg cagtcctgcg attcggcggg
     1321 tgggcactcg agctcgaggc gaacaccgcg cagggctgcc accagcgagg cggccgcggc
     1381 caggtgaatc gaggtggccc cgtctgcaag gcgatgttcc agcctggtgc ctgactcgtt
     1441 ggccggtggg accctgaccg ccaccccgcg gtggtcgtat ccccagttgg cgtagtagcc
     1501 gttgagcgat cccggcttga ggcgcttgta ggcattcacc gtgggcgcga cgagggcgga
     1561 catcgacacg tggtcgtgga ccaggccggc gatcgcctgg cgcgccaact cgctgagccc
     1621 gtctcgtgcg gcgggatcgc tgaaggcgtt cttcccatcg cggtcgacca gagaaatgtt
     1681 gacgtggatc ccgctccctc cccgctcggt gagcggcttg cccaggaaag agaggaggta
     1741 gccacgtttg gctgccacct cgcgggccat caccttgaac aagaaggcat cgtcggccgc
     1801 ctgcaaagcc ttgtcgtatc ggagggtgag ctcgaattgg ccatcgtcat actcggaatt
     1861 gaccgattcc aggtcgaggc ccatgtcgtg ggcggtctcc atgatggcct ggatggtgcc
     1921 ggtggggtca accgcagtgc cggtgccata aacgtacgag cctggggtgt ggattggccg
     1981 ccaaccgccg tctccgtcgg gctcgaagag gaatgcttcc aactcgaagc cgatcatcgg
     2041 ggtcagtccc agttcttccc actcaccgac aactctgtcc agaatcgagc gcggcgccag
     2101 ctcgacgggt gctccatcct tggtgatgga ggcgacgacc acgccggttc cgttctccca
     2161 tcccggtcga acgtcatcca tcgagaatcg ggcctcgacg tcggggaaac tctcgtgatg
     2221 ggctccggga tgggggatca ttacccggtt cagttgcagg gagaagaggg ccacgcagtg
     2281 gttgctcccc tcggccgccc gttgcccgga caggtacttg ccgcgggcca gaccgagatg
     2341 gtcgggccag aggaagcgaa cccggtcgta gctgttgtca ctcagaaggc tgtctccttc
     2401 tcggcgccgg acaggcgcgt gctctcacgg tcggagacag tatcaaccgg ccgcttgata
     2461 aactcaatgg atgcgggctc tcgtcatcga acacgacccg ctctcgactc ccgatcgggt
     2521 gggcgagcat ctggagcggc gcggcgcccg tctcgagcct tttgtcgtcg ttgaggacat
     2581 caatcacccg gaagtgacgg cgacgttccc ggacaacggg cacgacctgg tggtcctgat
     2641 gggctcaccg tggtcggtgt acgaggaacg gctgcaaggc tgggtcggcc ccgagctcga
     2701 attcatccgg cgccaggcca gttccggtgt gcccattctc ggcatctgtt tcggcgcaca
     2761 agcgatgtcc gctgcgatgg gggggaaggt cactcgggcg agccgcccgg aatatggctg
     2821 ggtgagcatc gaatctcagg tcgacgagat cgccggcggg ccctggtttc agtatcacca
     2881 tgacgagttc acccttcctc ctggcgcgat cgaattggcc cgcaacgcct cgggcctgca
     2941 ggcattccaa tcggggaaga acctcgccgt ccagttccac cctgagatga ccgccgagct
     3001 gatcgcctcg tggtgcgagg tcgatggtgg tgaggagatg gtggatgtcg gcatcgaccc
     3061 tgacaaggtg atcgaggaga gccgcctgct ggagaagacc acccaaccct cgttggagcg
     3121 gatgttggac tggt
//
LOCUS       DBNHXU010000361         1510 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4108488, whole genome shotgun sequence.
ACCESSION   DBNHXU010000361 DBNHXU010000000
VERSION     DBNHXU010000361.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1510)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1510
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4108488"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..291
                     /locus_tag="AC161X_08885"
     CDS             <1..291
                     /locus_tag="AC161X_08885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989708.1"
                     /translation="DDDSSPWHTIAEIRAPDRIGLLHSQAMAFAAAGADVSAARITTE
                     GSTAIGRFDLNGSVGGKLTQVDKDLIRSFLVSGGSASRGRSTNLVRRLVKGR"
     gene            complement(278..673)
                     /locus_tag="AC161X_08890"
     CDS             complement(278..673)
                     /locus_tag="AC161X_08890"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012890549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4989709.1"
                     /translation="MAITYQWRGSFHSGEVEALHAEGFGHEPVAYDWESQLESHSLGW
                     VCAREGEDLIGFVNVAWDGAGHAFILDTLVAVPARRSGVGKQLVAIAVENARHAGCEW
                     LHVDFEDELKTFYYEACGFEPTNAGLIAL"
     gene            699..>1510
                     /locus_tag="AC161X_08895"
     CDS             699..>1510
                     /locus_tag="AC161X_08895"
                     /inference="COORDINATES: protein motif:HMM:NF027787.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolB family protein"
                     /protein_id="HIR4989710.1"
                     /translation="MIRAMFPDLATKRRRAGVIVLFTLAACVGQPAADETTTSTAIAV
                     ATTSPRSSLEVGSLQGTIVYSTESAGNDDVFLVKLGDGDPIRLTDGPEKEFDPDLSAD
                     GASIAYRNNPDVASDAADIWVMDSDGQNKRNLTDAPELSNWAPAWTPDGHIVFSSMRG
                     GSGGLELWSMTADGGDVQQVSEGWCEYPDVSPDGSEFVCAEAVGSRYDIVIVDGDGGR
                     RSVTTTPVTEFGPSWSPDGQWIVFSRDTGEHWELYRIRPDGSDEELIAQEGV"
BASE COUNT          286 a          464 c          481 g          279 t
ORIGIN      
        1 gatgacgact cctcaccctg gcataccatc gccgagatcc gggctcccga tcgcatcggg
       61 ctgctccact ctcaggcgat ggcgtttgcc gccgccgggg ctgacgtttc tgcagccagg
      121 atcaccaccg aagggagcac cgcaatcggc cgcttcgatc tcaatggcag tgttggcggg
      181 aagctgacac aggtcgacaa ggatctgatc cgctccttcc tcgtctcggg tggctccgcg
      241 agccgcggac ggagcaccaa cttggtgaga aggttggtca aagggcgatg agaccggcgt
      301 tggtcggttc gaacccgcaa gcctcgtagt agaaggtctt gagctcgtcc tcgaagtcga
      361 cgtggagcca ctcgcatcct gcgtgtctcg cattctcgac ggcgatcgcc acgagctgct
      421 tgcccacccc gctccgacga gctggaacgg cgaccagggt gtcgaggatg aacgcgtgac
      481 cggcgccatc ccaggcgaca ttgacgaagc cgatcaggtc ctcgccttca cgggcgcaaa
      541 cccaccccaa actgtgactc tcgagctggg actcccagtc gtaggcgacg ggctcatgtc
      601 caaaaccttc ggcatgaagt gcctcgacct cgccgctgtg gaaggatcca cgccattgat
      661 aggtgatcgc cattgatcca acttagggaa caagcaccat gattcgggca atgtttccgg
      721 acctagccac gaagcgccgg cgcgccgggg tgatcgtgct gttcactctg gctgcctgtg
      781 ttggccagcc agcggcagac gagacgacca cgagcacggc tatagcggtg gcgacgacct
      841 ccccccggtc atctctcgaa gtcggatcgt tgcaggggac catcgtctac tcgacagaaa
      901 gcgccgggaa cgatgacgtc ttcctggtga aactcggcga tggcgatccc atccgtctca
      961 ccgatggccc tgagaaagag ttcgatccgg atctttcggc ggatggtgcc tccatcgcct
     1021 atcggaacaa ccccgatgtg gcaagcgatg cggccgacat ctgggtcatg gattccgatg
     1081 gtcagaacaa gcgaaacctg accgacgccc cagagctctc caattgggca ccggcgtgga
     1141 cgcccgatgg gcacatcgtt ttctccagca tgcgtggcgg tagcggtggc ctcgagttgt
     1201 ggtcgatgac ggccgacggc ggcgatgtgc aacaggtgtc ggaggggtgg tgcgagtacc
     1261 cggacgtgtc acctgacgga tccgagttcg tctgcgccga agctgtcgga tccaggtacg
     1321 acatcgtgat cgtcgatggg gacggcggcc gtcgatcggt caccacgaca ccagtgaccg
     1381 agttcggccc gagctggtct cccgacgggc agtggatcgt cttcagtcgc gacaccggtg
     1441 agcactggga gctgtatcgg atccggcccg atggttccga cgaggaattg atcgcccagg
     1501 agggtgtgtt
//
LOCUS       DBNHXU010000362         2163 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3711375, whole genome shotgun sequence.
ACCESSION   DBNHXU010000362 DBNHXU010000000
VERSION     DBNHXU010000362.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3711375"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="AC161X_08900"
     CDS             complement(<1..207)
                     /locus_tag="AC161X_08900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989711.1"
                     /translation="MPERGHAAVEFALAVGLLLLPVAITVLAFGPWSERRVVAEAAAA
                     EAARTAVVSLDLSRGSQVVSEIGGN"
     gene            complement(200..595)
                     /locus_tag="AC161X_08905"
     CDS             complement(200..595)
                     /locus_tag="AC161X_08905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989712.1"
                     /translation="MDPHLVDRRLRERGVTSVQFLLAGALSLVLFLVFANLIVVQYGR
                     GAIRSALDQGARVGAVTGALEGCEQRVEAVLSQILGGDMGESTEVECTIDGDLVVAHG
                     SAVFPSWTPFTSDFVVDLSAQATLEPNRA"
     gene            complement(565..744)
                     /locus_tag="AC161X_08910"
     CDS             complement(565..744)
                     /locus_tag="AC161X_08910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989713.1"
                     /translation="MPDVNQILRAMREDDRGLNTAELMGNAALAIAALVVIWGALQAL
                     GVDIVSWIRTSLIGG"
     gene            complement(758..1684)
                     /locus_tag="AC161X_08915"
     CDS             complement(758..1684)
                     /locus_tag="AC161X_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012694.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR4989714.1"
                     /translation="MTGSPLVLLAGMASALAAGLLVHHLVPPPRSLASRIHPYRAPAR
                     SMSRANRVGGPISEVFGPIVRQMAETLGRLLERSGDQVTILKLRQAGWYRQLPEEEMV
                     SAYRVTQLRSTAVAVGLAVVAGWAIGASLLQRLILIGLGLLIGATRQRSKIDKAIESR
                     RELMRIEVYTVNQLLAMRVRAGGGVVHAVTSTVARGRGEVINELADALRLHRAGWRAG
                     DAFRRIAELTPEPFCSRTYRLLASAEERGADLAGALLDLAEDVRETRRETIKRSATKR
                     RAAMLVPTIAILAPVLILFVAAPLPSLITGWR"
     gene            complement(1681..>2163)
                     /locus_tag="AC161X_08920"
     CDS             complement(1681..>2163)
                     /locus_tag="AC161X_08920"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR4989715.1"
                     /translation="ENLAGFGPAPLRSAFQGFGVYSRSLGVVPALEMVKSDLADPTSD
                     RVIEVLILAYERGGSVVPEILSDLAEATTRDIWTLEQIRTEALEQKINARVVFVLPWF
                     VLIAMTAKDGAFREFYSTTAGLTVAVIGGVMSLLGIAIASRLGSQPAEPRVFTSGRAP
                     "
BASE COUNT          446 a          740 c          627 g          350 t
ORIGIN      
        1 gttgccacca atctccgaaa ccacctggct accccggctg agatccagtg aaaccaccgc
       61 agtcctcgcc gcctcggcgg cagcggcctc agcgaccaca cgccgctccg accagggccc
      121 gaaagccagc accgttatcg caaccgggag cagcaacagc ccgactgcga gggcgaactc
      181 gacggcagcg tggcctctct caggcacgat tcggctccaa cgtcgcctgg gcgctgaggt
      241 ctacgacaaa gtctgacgtg aacggagtcc acgatgggaa gacagcactg ccatgggcaa
      301 caaccaggtc gccgtcaatg gtgcactcga cttccgtcga ttcacccata tcgcccccca
      361 gtatttggct cagaaccgct tcgactcgct gctcgcatcc ctcgagggcc ccggtgacag
      421 cacccaccct cgctccttgg tcgagtgcgg agcgaatcgc gcctcgtccg tactggacca
      481 cgatcaagtt ggcgaacacc aggaacagga cgagcgagag tgctcccgcc aggaggaact
      541 ggaccgaggt gacccctcgc tccctcagcc tccgatcaac gaggtgcgga tccaactgac
      601 tatgtccacc ccgagtgcct ggagggcgcc ccagatcacc accaaggcag caatcgccaa
      661 ggcggcgttc cccattagct cggcggtgtt tagcccccgg tcgtcctcgc gcatcgcgcg
      721 caggatttgg tttacatcgg gcatggtcgg cctcctttca tcgccagccc gtgatcaaac
      781 ttggaagcgg cgcagcgacg aacagaatca agaccggagc caggatggcg atggttggca
      841 cgagcatcgc cgcccgcctc ttggtcgccg acctcttgat cgtctcacgc cgggtctctc
      901 gaacatcttc cgcaagatcg agcaaggccc cggccagatc tgccccccgc tcttcggcag
      961 aggcaagaag gcgataagtc cggctgcaga atggctcggg ggtgagctct gcaattctcc
     1021 gaaacgcgtc accggcccgc catccggcac gatgcaatcg cagggcgtcc gccaactcgt
     1081 tgatgacctc accgcgtccc cgagcgaccg tggaagtcac agcatggacg acgccacctc
     1141 cagccctaac ccgcatggcg agaagctgat tgacggtgta cacctcgatc cgcatcagtt
     1201 ctctgcggct ctcgatcgcc ttgtcgattt tcgatcgttg tcgggtcgcg ccgatcagca
     1261 accccaggcc gatcaggatc agacgctgga gcaaagacgc cccgatcgcc catcctgcca
     1321 ccacggcaag tccgaccgca accgccgtcg acctcagttg cgtcacccga tatgcagaaa
     1381 ccatctcttc ttctggaagc tggcgatacc aacccgcctg ccgaagtttg aggatcgtga
     1441 cctggtcgcc tgatctctcg agcagccgcc ccagggtctc agccatctgg cgaacgatcg
     1501 ggccgaacac ctcagaaatc ggacccccga cgcggttcgc ccgtgacatc gatcgggcgg
     1561 gggcacggta gggatggatg cgagacgcca gcgatcgagg aggcggaacc agatgatgga
     1621 ccaagagccc tgcggcgaga gctgacgcca tcccggccag aagcaccagt ggtgagccgg
     1681 tcatggggcc ctgcccgagg tgaacaccct cggctcggcg ggctgactgc caagccggga
     1741 cgcgatcgcg ataccaagaa ggctcatcac gccgccaatg acagccactg ttaggccggc
     1801 agtggtcgag tagaactcac ggaatgcacc gtccttggcg gtcatcgcga tcagaacgaa
     1861 ccaagggagt acaaagacca cccgggcatt gatcttctgc tcgagtgcct cggttcggat
     1921 ctgctcgaga gtccatatgt cccgggtcgt cgcttcggcc aaatcggaca ggatctcagg
     1981 taccactgac cctccccgct cataggcgag gatcagcact tcgatcaccc ggtccgaagt
     2041 cggatctgcc aagtccgact tcaccatctc caaggcaggc accactccaa gggatcgcga
     2101 gtagacgccg aagccctgga acgcggagcg gaggggtgct ggtccgaacc cggcgagatt
     2161 ctc
//
LOCUS       DBNHXU010000363         3158 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5503220, whole genome shotgun sequence.
ACCESSION   DBNHXU010000363 DBNHXU010000000
VERSION     DBNHXU010000363.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3158)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3158
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5503220"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1979)
                     /gene="acnA"
                     /locus_tag="AC161X_08925"
     CDS             complement(<1..1979)
                     /gene="acnA"
                     /locus_tag="AC161X_08925"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006747496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003994 - aconitate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HIR4989716.1"
                     /translation="MKGFTLETALGSRQIARIDDLEGADRLPYSIKVLLESALRNLDG
                     KNVTEEDVRRIAAYDARSVGEQEIPFVPGRVVLQDFTGVPAVVDLAAMRSAIVRLTGD
                     NAAAQKVNPLIPADLVVDHSVQVDAFARSDALTINSRKEFERNQERYEFLKWGQSAFA
                     NFRVVPPATGIVHQVNLEYLAKVVWDDGHQLYPDSLVGTDSHTTMINGLGVVGWGVGG
                     IEAEAAMVGQPIYMLLPEVVGFRLTGKLPEGSTATDLVLRVTEMLREHGVVDKFVEFH
                     GTGLADMPVANRATIANMAPEYGATVGFFPVDEQTLNYLRLTGRDDKLIDTVEQYHKA
                     QGMWRDDSHQITYSAELSLDMSTVEPSLAGPKRPQDRVALSAMSTQWTADLTTLFGKN
                     GGAKTSTVVSWEDEGGQPSGSVALEAQAKVEVEMDGESFDLGDGDVVIAAITSCTNTS
                     NPDVMVGAGLVARKARERGLTRKPWVKTSLAPGSKVVTEYLTSSGLLPDLEELGFYVV
                     GYGCTTCIGNSGPLPGPISEAIHTGDLVTASVLSGNRNFEGRISPDVRANYLASPPLV
                     VAYALAGTVAWDPITEPLGVDQQGNDVFLRDIWPTQEEIAEIVSAHVHQSQFIDKYSD
                     VFAGSDEWRVISTPESDLYPWDDESTYVQEPPFFTT"
     gene            complement(2112..2828)
                     /locus_tag="AC161X_08930"
     CDS             complement(2112..2828)
                     /locus_tag="AC161X_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HIR4989717.1"
                     /translation="MDTETLDSRISDLTSSLGDPTRRAIYIAVRESVEPLTTSHVAGL
                     FDLHPNVARHHLDRLADDGYLKVSHRRSEGKNGPGAGRPAKCYEATAKEVSVHFAPRR
                     FEMLVEMLIRVLDEVAPENVAQIAEDVGRAYGRELAAEIGQPEQPGYDGAIQAVASAM
                     TGLGFSIDPDVPGQRLLTSHCPFGEAATSHPEVICSLDRGIVAGMFGAMSYPCNPVVI
                     PHKALEDDCVTRVPVTIGNA"
     gene            2898..>3158
                     /locus_tag="AC161X_08935"
     CDS             2898..>3158
                     /locus_tag="AC161X_08935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989718.1"
                     /translation="MAADNQIFDAVASIESPLFVGRTLGELGAVKKVDRSLTGKLSVE
                     LLLPTSHTSDLLDQRLKEALQDHGRGVDIDIEVMSEEAAIQLI"
BASE COUNT          574 a          955 c         1018 g          611 t
ORIGIN      
        1 gtcgtgaaga aggggggctc ctggacgtag gtggactcgt cgtcccaggg gtagaggtcg
       61 gactccggag tgctgataac acgccactcg tccgaacctg cgaacacgtc ggagtacttg
      121 tcgatgaact ggctctggtg gacgtgagcg gagacgattt cggcgatctc ttcctgagtc
      181 ggccagatgt cgcggaggaa cacatcgttt ccttgctggt cgacacccaa cggctcggtg
      241 atcgggtccc aggccacggt tccagccagg gcataggcca cgaccagggg cggggaggcc
      301 aggtagttgg ctcggacgtc cggagagatg cggccttcga agttccggtt gcccgacaac
      361 acagatgcgg tgaccaggtc gccagtgtgg atcgcctcgg agatgggtcc gggcaggggg
      421 cccgaattgc cgatgcaggt tgtgcaccca taaccgacga catagaaccc gagctcctcg
      481 aggtcaggca gcaatcccga gctggtgagg tactcagtga ccactttgga ccctggcgcc
      541 agggaagtct tcacccaggg ctttcgggtc agcccccgct cccttgcctt gcgagcgaca
      601 aggccggctc ccaccatcac atctgggttc gaggtgttgg tgcatgaagt gatcgccgcg
      661 atcaccacgt ccccgtcacc gagatcgaag gattcgccgt ccatctccac ttcgaccttg
      721 gcttgcgcct cgagtgccac cgaacccgac ggctgaccgc cttcgtcttc ccaactgaca
      781 acggtggacg tctttgcccc gccgttctta ccaaagaggg tggtcaggtc ggcagtccac
      841 tgcgtgctca tcgcagagag ggccacacga tcctgtggcc gcttgggccc ggccagagac
      901 ggttcgactg tggacatgtc gagggagagc tcggccgagt aggtgatctg gtggctgtcg
      961 tcccgccaca tgccctgggc cttgtggtac tgctcgacgg tgtcgatcag cttgtcgtcg
     1021 cggccggtga gccggaggta gttcagggtc tgttcgtcga ccgggaagaa tccaacagtg
     1081 gcgccgtact ccggggccat gttggcgatg gtcgccctgt ttgccactgg catatcggca
     1141 agccccgtgc cgtggaattc gacgaacttg tccaccaccc cgtgctcccg gagcatttcg
     1201 gtcactcgaa gcacgaggtc ggtggcggtg gatccctcgg gaagctttcc ggtcagccgg
     1261 aacccgacga cctcgggaag caacatgtag atcggctggc cgaccattgc cgcttcagct
     1321 tcgatgcccc cgactcccca gccgactact cccaggccgt tgatcatcgt ggtgtggctg
     1381 tctgttccca cgaggctgtc cggatagagc tgatgcccgt cgtcccagac caccttggcc
     1441 agatactcga ggttgacctg gtggacgatg ccagtggccg gcgggacgac gcgaaagttg
     1501 gcgaaggcgg actggcccca cttgaggaac tcatatcgct cctggttcct ctcgaactct
     1561 ttgcgggagt tgatggtgag agcatcggat cgtgcaaagg cgtcgacctg aaccgagtga
     1621 tcgaccacca ggtcggcggg aatgagtggg ttgaccttct gggcggcagc attgtctccg
     1681 gtcaggcgga cgatcgccga gcgcatcgcg gcgagatcga ccacggccgg cacaccagtg
     1741 aagtcctgga gcaccactcg acccggcacg aaggggattt cctgctcgcc gacgctccgg
     1801 gcgtcgtagg cggcgatccg ccggacatcc tcctcggtca cgttcttgcc atcgaggttg
     1861 cggagggcgg actcgaggag cacctttatc gagtagggga gccggtcggc accttcgagg
     1921 tcgtcgattc gggctatctg gcgggacccg agtgctgttt cgagggtgaa ccctttcata
     1981 ggcccaggtt accggtgtgg cccgcgtgtt cctcgggcca gttgccggtt atcagtcatc
     2041 cgttttccgt tttgagaatt cgtgtcgatc ttgaactggg aacggagaac agagaacgac
     2101 ggggcgacgc ttcaggcgtt gccgatcgtc actggcaccc gggtcacaca gtcatcctcc
     2161 agggccttat gcggtatgac caccgggttg cacggatacg acatcgctcc gaacatcccg
     2221 gcgacgattc cgcgatcgag cgagcaaatc acctcggggt ggctggtcgc cgcctcgccg
     2281 aacgggcaat gtgaggtgag aagccgctgc ccgggaacat cgggatcgat cgagaagccg
     2341 agcccggtca tcgcactcgc gaccgcctgg attgccccgt catagccggg ctgctcgggc
     2401 tggccgatct ccgccgccaa ttcccggccg taggccctgc cgacatcttc agcgatctgg
     2461 gcgacgtttt ctggcgccac ctcgtcgaga acccggatca acatctcgac cagcatctcg
     2521 aatcgcctcg gtgcgaagtg gaccgagacc tccttggccg ttgcttcata gcacttggcc
     2581 gggcgaccgg ctcccgggcc gttcttgccc tcggagcgac ggtggctcac tttcaggtag
     2641 ccatcatcgg ccagacggtc gaggtgatgg cgggcgacgt tggggtggag gtcgaagaga
     2701 ccggccacgt gagaagtggt tagcggctcg accgattcgc gaaccgcgat gtaaatcgcc
     2761 cgacgggttg gatcacccag ggacgaggtg agatcggaga tccgactatc gagggtttca
     2821 gtgtccatga ggggtaccaa caggtcaaaa ctatagtttc aagggccttc tatccactgt
     2881 cgccgtaggg gaaatcaatg gccgcggaca atcagatctt cgacgcggtc gcatcaatcg
     2941 aatcccctct gttcgtcgga cgcaccctcg gggagcttgg cgccgtgaaa aaggtcgacc
     3001 gatcactgac tgggaaactg agcgtggagc tgcttcttcc cacgtctcac acctccgact
     3061 tgttggatca acgactcaag gaggcgctcc aagaccatgg ccgcggcgtc gacatcgaca
     3121 tcgaggtcat gagcgaggag gcggcgatcc agttgatc
//
LOCUS       DBNHXU010000364         2104 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_541794, whole genome shotgun sequence.
ACCESSION   DBNHXU010000364 DBNHXU010000000
VERSION     DBNHXU010000364.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2104)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2104
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_541794"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..953
                     /locus_tag="AC161X_08940"
     CDS             <1..953
                     /locus_tag="AC161X_08940"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR4989719.1"
                     /translation="DWSHIQASPAHTDAAVKALGDSGMRAVFAYGFPWWGKWEERQPS
                     WFVRTATEHFSSNSQLLSLALAAPGPEFVDFEVARDHWKLARETGARITAHVGVGTYG
                     QDAKVQEMGEAGLLGPDTTYIHCTTLNDTEIQMIVDTGGTVSLASPVEMMMGHGMPPI
                     QKFLDRGLRPSLSIDVETNVPADMFNQMRSVVALQRALVNGTDKSQISMREVLEWATI
                     EGAKANGLDSKVGTLTPGKQADVIMLRTKRVNVTPLNDPATAVVAGMDTSNVDTVLIA
                     GRVMKQNGKLLHVDWPAVRRMVGESRDYVVEKSGFKLPKI"
     gene            963..>2104
                     /gene="lhgO"
                     /locus_tag="AC161X_08945"
     CDS             963..>2104
                     /gene="lhgO"
                     /locus_tag="AC161X_08945"
                     /EC_number="1.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-2-hydroxyglutarate oxidase"
                     /protein_id="HIR4989720.1"
                     /translation="MRYDVCIVGAGLVGLSVGRAVLEKYPGSSVVLLDKEARVAAHQS
                     SHNSGVAHSGLYYRPGSLKARLCVEGRAELERLCDSASIPFRRLGKLVIATDSAELEA
                     LDELERRGRANGLTGLRRLDLAGIREIEPAATGLQGLHVPEAGVVDFPGVAAHLAAEL
                     QRNGAEVRTGLEVTAIDHEADGARIVAGDQEVRARIMVNCAGLHSDRVAALAGVESDI
                     RIVPFRGEYYTLDPEVSHLVKGLVYPVPDPRFPFLGVHFTRRVDDSVEVGPNAVLALG
                     REQYRGSPIGWGDLREVMALRGFWKLAGRHWLTGGAEIFRSKSRALYARSAQRLIPAL
                     RSDHLGQGGVGVRAQALGRDGRLVDDFVIEEVGATLHVLNAPSPGAT"
BASE COUNT          365 a          631 c          702 g          406 t
ORIGIN      
        1 tcgactggtc tcacattcag gcctcgcctg cccacactga tgccgcggtc aaggcattgg
       61 gagactccgg gatgcgcgcc gtgtttgcct acgggtttcc ctggtggggg aagtgggaag
      121 agcgccagcc cagttggttc gtgcgaactg cgacggagca cttctcttcg aacagtcagc
      181 tcctcagtct ggccctcgcc gcgccgggtc cggaattcgt cgacttcgaa gtggcacgtg
      241 accattggaa gctggcacgt gagaccgggg cgaggatcac cgctcatgtc ggcgtgggga
      301 cctacggcca ggacgccaag gttcaggaaa tgggtgaggc cgggcttctc gggcctgaca
      361 ccacctatat ccattgcacc acgctgaacg acaccgagat ccaaatgatc gtcgacaccg
      421 ggggcacggt ctctctggcc tccccggtcg agatgatgat gggacacgga atgcctccga
      481 tccagaagtt cctggaccgt gggctgcgtc ccagtctcag cattgatgtc gagaccaatg
      541 tgcccgcaga catgttcaat cagatgcgct cggtcgtcgc tttgcagcgg gcgctcgtca
      601 atgggactga caagtcccag atctccatgc gtgaggtcct cgagtgggcc acgattgagg
      661 gggcgaaggc caatggcctc gactccaaag tcggaaccct cactccgggc aagcaggccg
      721 acgtgatcat gctccgcacc aaacgggtca acgtcacccc tctcaacgat cccgccaccg
      781 ccgtggttgc cgggatggac acctccaacg ttgacacggt cttgattgcg gggcgggtga
      841 tgaagcagaa cggcaagctg cttcatgtcg actggccggc ggttcgccgg atggttggcg
      901 aatcgcggga ctacgtggtc gagaagtcgg gtttcaaact ccccaagatt taggcggggg
      961 agttgaggta cgacgtctgc atcgtcggag ccgggttggt cgggttgagc gttggccgag
     1021 ccgtcttgga gaagtatccg ggctcctcgg ttgtgttgct cgacaaggag gctcgggtcg
     1081 ccgcccatca gagcagtcac aacagcggcg tcgcacattc cggtctctac taccgccccg
     1141 gatccctgaa agcacggctt tgtgtggaag gccgcgccga gctggagcga ctttgtgact
     1201 cagcgtccat cccctttcgt cgtctgggga agctggtaat cgccaccgat tcggccgagc
     1261 tcgaggccct cgatgagttg gagcggcgcg ggcgagccaa cgggctgacc gggctgcggc
     1321 ggctggatct tgcaggcatt cgtgagatcg aaccggcggc aaccgggcta caagggcttc
     1381 atgtgcccga agccggtgtt gtcgactttc ccggggtcgc cgcccacctt gctgctgaac
     1441 tgcaacgtaa cggcgccgag gtgcgcactg gccttgaagt caccgcgatc gaccatgaag
     1501 ccgatggagc gcggatagtt gccggtgacc aggaagttcg agcccggatc atggtcaatt
     1561 gcgccgggct ccactccgat cgtgttgcag cactggccgg tgtggagagt gatatccgca
     1621 tcgtcccgtt ccggggcgag tactacaccc tcgatcctga ggtctcccac ctggtcaagg
     1681 gactcgtcta cccggtgcca gacccacggt tcccgttcct cggcgttcat ttcacccgcc
     1741 gggtggacga ttcggtggag gtggggccga acgcggtgct cgctctgggc cgcgagcagt
     1801 atcggggatc gccgatcggc tggggagact tgagggaggt gatggccctc cgaggatttt
     1861 ggaagctggc gggcaggcat tggctcacgg gtggggccga gatattccgg tcgaaatcgc
     1921 gggctctcta cgcccgttcc gcccagcggc tgattcccgc cctcagaagt gatcacctcg
     1981 gccaaggtgg ggtcggcgtg cgtgcgcagg cgctgggtcg tgatggccga ttggtcgatg
     2041 acttcgtcat cgaagaggtc ggcgcgacat tgcatgtcct caacgcccca tcgcccgggg
     2101 ccac
//
LOCUS       DBNHXU010000365         1672 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5107223, whole genome shotgun sequence.
ACCESSION   DBNHXU010000365 DBNHXU010000000
VERSION     DBNHXU010000365.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1672)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1672
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5107223"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..914)
                     /locus_tag="AC161X_08950"
     CDS             complement(<1..914)
                     /locus_tag="AC161X_08950"
                     /inference="COORDINATES: protein motif:HMM:NF014081.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HIR4989721.1"
                     /translation="MTSTRIEAKRLIPGRGDPIADAVVLLENGVIKFAGAASDGPTAD
                     GADTVTTDTVMPGMWECHGHFIGGYTANIAEIHTLRPQLGAMRVTADARRALDAGFTS
                     VREMGGMGTFLGRAIEEGQVVGPNVYASGSMLSMTGGHGDEHGMELGFARASAARQWG
                     EDGICDGPDECRAGVRRMLRLGAKVIKVHASGGVMSELDDPQLPQFSKAELEAIVDEA
                     TRMERLVGAHCHGKRGIMAALEAGVKTIEHGTYLDDEAADAMIESEAILVPTRWIVEY
                     LMTEGEKNGMPEYARVKGQVASQHHATAV"
     gene            complement(911..1372)
                     /locus_tag="AC161X_08955"
     CDS             complement(911..1372)
                     /locus_tag="AC161X_08955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989722.1"
                     /translation="MFRFIFKTIRLAFLTMLGTAAVAKFMLKSNAGPETQEIDLVTIF
                     GGDNLISSANPFYGGKILTLYGGTILDLRKATPAPTGIYLDALVWMGGVDLVVPEGWR
                     VEFTGKVIFGGFTDATKTTADPDAPTVRIGGMILFGGLRATTRAPLEVVTP"
     gene            complement(1403..>1672)
                     /locus_tag="AC161X_08960"
     CDS             complement(1403..>1672)
                     /locus_tag="AC161X_08960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1684 domain-containing protein"
                     /protein_id="HIR4989723.1"
                     /translation="VLWLDAYGGGVFLPFRDTTSGASTYGGGRYLLDTVKGADLGHAG
                     EEVVLDFNYSYHPSCVHSPRWACPLAPPGNHLGFAVEAGERLSTG"
BASE COUNT          295 a          531 c          534 g          312 t
ORIGIN      
        1 accgcggtgg catggtgctg ggaggcgacc tgccctttca ctctcgcgta ctcgggcatc
       61 ccattcttct cgccttcggt catcaggtat tcgacgatcc accttgttgg caccaggatc
      121 gcctcgctct cgatcatggc gtcggcggcc tcgtcgtcga gataggtgcc atgctcgatg
      181 gtcttcactc ctgcctcgag ggcggccatg atgccgcgct tcccatgaca atgcgcgccg
      241 accaggcgtt ccatgcgggt ggcctcgtcg acgatcgctt ccaactccgc cttggagaac
      301 tgggggagtt ggggatcgtc caattcgctc atcacgccac ccgaggcgtg caccttgatc
      361 accttggcgc caaggcgaag catgcgacgc acgcctgccc ggcactcatc tggcccgtcg
      421 cagatgccat cctcccccca ctggcgggca gcggaagcac gagcgaagcc aagttccatc
      481 ccgtgctcgt caccgtggcc gccggtcatc gagagcatcg atccgctggc atagacgttc
      541 ggcccgacga cctgaccttc ttcgatggcg cgcccgagga aggtccccat gccgcccatc
      601 tcccggaccg aggtgaatcc tgcgtcgagg gcccgtctgg catcggcagt gacacgcatc
      661 gcccccagtt gaggtcgaag ggtgtgtatc tcggcgatgt tggcggtgta gccaccgatg
      721 aaatggccat ggcactccca catgcccggc atcacggtgt cggtggtcac tgtgtctgct
      781 ccgtccgcag tagggccatc agatgcggct ccggcgaatt tgatgacgcc gttctcgagg
      841 aggaccacag cgtcggcgat cgggtcgccg cggccaggga tgagacgctt ggcctcgatc
      901 ctggtgctgg tcatggggtc accacttcca ggggagcccg ggtggtcgcc cgcaggccgc
      961 cgaagaggat catcccgccg atccggaccg ttggtgcgtc gggatcggct gtggtcttgg
     1021 tggcgtcggt gaaaccgccg aagatcacct tgccggtgaa ctcgaccctc cagccctcgg
     1081 ggaccaccag atcaacccca cccatccaaa ccagggcgtc gaggtagatc ccagtcggcg
     1141 ccggtgtggc tttgcgcagg tcgaggatcg tgcctccgta gagggtcagg atcttgcccc
     1201 cgtagaacgg attggctgag gagatgaggt tgtcgccgcc gaagatcgtg accaggtcga
     1261 tttcctgggt ctccgggccg gcgttggatt tcagcatgaa cttggcgact gcagcagtcc
     1321 ccaacatggt gaggaaggcg agacgaatcg tcttgaaaat gaatctgaac acggctctac
     1381 cttacgtcac cttcaggttg cgttagccgg tggagaggcg ttctccggcc tccacagcga
     1441 acccgagatg gttgcccggc ggagcaagag gacacgccca gcgaggcgag tggacacatg
     1501 aggggtggta ggaatagttg aagtcgagga cgacctcctc gccggcgtgg ccaaggtcgg
     1561 cgcccttgac agtgtcgagc aggtaacggc ccccgccata ggttgaagca ccgcttgtgg
     1621 tgtcccgaaa cgggagaaac accccacctc cataggcgtc gagccagagc ac
//
LOCUS       DBNHXU010000366        13859 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2924324, whole genome shotgun sequence.
ACCESSION   DBNHXU010000366 DBNHXU010000000
VERSION     DBNHXU010000366.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 13859)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 13859)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13859
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2924324"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC161X_08965"
     CDS             complement(<1..395)
                     /locus_tag="AC161X_08965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989724.1"
                     /translation="MSNKQRVFFDWTLDTLLYLVVLGFFSEYTDGVTIDSFTLIFLAS
                     LVLYVLTYVTFGFKRRVGVWYSSRPERRIAHFFSIWAILFFSKFIFLEVLDLVFGDAV
                     DFANFLWLMAVLVVVVVLGMVMELIYSKLG"
     gene            770..2794
                     /locus_tag="AC161X_08970"
     CDS             770..2794
                     /locus_tag="AC161X_08970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989725.1"
                     /translation="MGTMWRLVMFAVLATVCLPMSVAGAQQDAAQLSATVGVEGWVSG
                     DEPLTVHATIDAALLVAGFLRVSYGGAVTEIEVDVPAGGSKTYDILLRPPFRNGSVQV
                     ILFDADGQRLTSQSIRPQVADDEVIVGVAGDSGLTTTLSALQTSIDGLSITALDISES
                     LPRADLEGLSYLVSRDVDPVVWEWVSDGGHLVTTRGELSETPLSLEPIGTVPGTDVEW
                     YTAGPTGEVFLIDGVAASEDWTRVIRPLPLKLIPTDQWGSTDGSLVQAATNSGDAGLA
                     NLPWLPFAMVAYLILIGPINFMVLKRMRRRELAWVTIPAMAAIAVLGFWASGRQRLDV
                     TSARHATVVVAGEQAYQRSIFVLAAGNAGAYSVAVPSAERYAVTDAFAALGGNVGPTS
                     AGRLSADGVAWQLPQLGVAAVETWQSANGSITVDSSLDGDTPILAVTNDTGLDIEHWG
                     AVIGGEVHVAQQGLATGQSATLRAGGAVAAWPGMSFGDVLVEQLQMWDDTGWQVISPM
                     GYAAQGEFSPSDSYVFGISMSTPMEAIVNGSAQMVDGPTVWTTPFSTQGFPSGKTMGR
                     VVGVGDWRQIEASQGNLWLETDRIVTSFDLPTDATNTRLGVAANFGAIGKLEMWNWST
                     GAFDAVELGQEFDPVSYRSASGEVIVRIFAANNGEPPYPQSVSVEWTRGT"
     gene            2776..3720
                     /locus_tag="AC161X_08975"
     CDS             2776..3720
                     /locus_tag="AC161X_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012031942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989726.1"
                     /translation="MDKGHMIEFESVEKRFGQAKALDGFDLSVPEGAILGLIGPNGAG
                     KTTSMQVMSTLLERDGGRVRVAGVDPADDAASVRRILGYMPDFFGFYESLTAYEYLEF
                     FAASQRIAPSARSGLISDLLALVDLTSKATADVNTLSRGMKQRLSLARSLLNDPQLLI
                     LDEPASGLDPRARVHLRELIAELSRMGKTVVISSHILSELEGICSHMAIVDRGKVVAQ
                     GLLEDIRLLLVASRRVTARMSDDVIDTVEDLLRARPEVSDMAVERGIIRFVLAGDRAE
                     SAATLRYIVESGVDVSEWRVDDAGLEELFMQLTEDGDV"
     gene            3717..4718
                     /locus_tag="AC161X_08980"
     CDS             3717..4718
                     /locus_tag="AC161X_08980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989727.1"
                     /translation="MNLNPVLAREAKQRFRSKRVVVVTTLWVGIIGGIAFLLYIAASE
                     VAVNGMGVGRLAAAAFMGRFMFHSITLLLLTAIILVVPGIAAPSIIGERERQTFQILQ
                     VTQMTPWQLVTGKLTASMMFAVVLLFAVAPVAAIPLLFGGTSLTDVLAALGMLLLTAV
                     TLASTATWMSSRAASTRGAVGMSYLIAFTLGFLTFVGLGAEMLVYSNLGSRDPMGRDG
                     REVFSILPNPYFGLVDAVLHPLDQTASTSDTPYLPFEYMLRLRQGVNPDLAEPAILGG
                     VAAGNAAGRDFPRPPLWAFNVVIYGGLTVFSLRRASVNVRAPSAKIRRPKRLKGTDA"
     gene            4711..6261
                     /locus_tag="AC161X_08985"
     CDS             4711..6261
                     /locus_tag="AC161X_08985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989728.1"
                     /translation="MLDTAIDHLISGARRRIWLSRTVSLATAAIGVGATMMAMLAAAA
                     HLWVLPWAEPAGLVVVGGCLITLLVVTFLVRPGPRETAQMVDRSLEGFDRVTTAYDLR
                     RSGPVAVMERRQVEAADRWADGRRPDPVATLMPARRLLVAAAVAVMGAAVLGLIPSIT
                     DRALAQREAARAAIAVEADRLEDLAELAPEEIAAELQVLADDLRELGSIDDALARLEE
                     ARRDLADNLEPTDLAERTALAGLQRSLEQSGASDGGDSPAETLGQLAAGAGELTPAEA
                     EQLASELSERAGDFAGVDNEVAESLAAAATALSGQAAGRSGSANSESALRAAADAVAA
                     SESASTAAAASQAGAAAAAEAQQRLQQGQGHGQGQGQGHGQGQGHGQGQGQGHGQGQG
                     QGPGGGGGGSGGQGQGGSSQGGTGSGSLDGAGGDQDPFSAPERATVFDPVVSGLGEEV
                     RVDIEGEGDGETQGTVDGQGIENLPLIPYADRFAEYRQTALDALSTSGIPAQSQDLVR
                     DYFLELEP"
     gene            6258..7253
                     /locus_tag="AC161X_08990"
     CDS             6258..7253
                     /locus_tag="AC161X_08990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011956623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989729.1"
                     /translation="MTTSARSDELTPEVFAQRLAAVRNEVEKVIVGQADLVDTVMMAT
                     LCEGHVLLEGVPGLGKTRLLATLGQALDVEFGRIQFTPDLMPADILGTKVLERGDFEF
                     SPGPIFNNLVLADEINRATPKTQSALLEAMQERNVTLGREVHPLPRPFMVMATQNPIE
                     QEGTYPLPEAQLDRFLFKAIVSFPPATDLVEILRRTTATTDPSAEAVLDGPRLEEMTR
                     LVRSVPIGIHLLEYAARLVESTHPASPLAPERIKAYVRNGASPRGAQAMILGGKAAAL
                     MAGRPNVAAEDLWRIAGQCLRHRLVLGYEAIADGVSADALIDDVLQAHPAPSVED"
     gene            7257..8126
                     /locus_tag="AC161X_08995"
     CDS             7257..8126
                     /locus_tag="AC161X_08995"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR4989730.1"
                     /translation="MLLAPETRSRLDRLALSPRGRVQALWSGGHASVRKGESLDFADY
                     RPYVPGDDFRKIDHNLWARLGQILIREYEAEEELPLRLAVDSSASMSLHGKHETALEL
                     AAMIAYLTLSGGDRVQPVSSPGPTGRAFAVGPSGRHLSVWPVIERWLESLEASGRAPL
                     GPAIRSLIGGSPTRGPVVLISDLFDDEWERALDSLFVSAGGLVLHVLAREELSPDFAG
                     DLRLRDIELGSVVDISTSEAVLTTYRASLDEFVAGAANRARRAGLDYLLVEAGSDAAE
                     TALRALLSAGVVR"
     gene            8123..9913
                     /locus_tag="AC161X_09000"
     CDS             8123..9913
                     /locus_tag="AC161X_09000"
                     /inference="COORDINATES: protein motif:HMM:NF024909.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vWA domain-containing protein"
                     /protein_id="HIR4989731.1"
                     /translation="MSFLVPVALGVAALAGPLLILYMLRSRRRQLDVTSTRLWQASEI
                     QVTSAVPWSRLRWTALLILQLLAIALFAFALARPFHSQRTLLGPHSVFVIDTSGSMAE
                     GDRLDRAKTEAVALAEDLSEGNLISVIGAGPHPQVLIGHARERDDVIATIESLRAGGG
                     EADLSEAIRLGRGLATPDRPTNVIVFSDGGEAPLPEEPVAGATHIPFHEIVDDVAVET
                     LTIDATGGVTRGLVAVANHSEADTSVELAVTVEGMPSTQMELDLGANQRAVETFELDA
                     APGSEVMVERLGEADGNPLDDRVWTVIDQNAARSVRVMGEGSPYLAALVSATPGFELG
                     SDDGDVLVVDRGVLPPIDRPAWLIRPDSTPEGLEITGLVENIPATFQAPGEPVLDNVD
                     LSDLVVAEAQNVAPRGWSPIVRSGDVPLILLGSVDGHRVVYFTFDLTHTNLPVQVSFP
                     ILGAALLNWLVGEAPGTVANGPAGEPIVISSPDGAQPVVTLPDGSQRTLPGDTASFTD
                     TSEPGIYRVGYVDEDGTVTAGQIAVRTFVATEAGAPVRDIVTSTGDSSAEEQGVVVRE
                     WGPWVVSALLALLAFEWWIGHQRPWLRQTA"
     gene            9910..12600
                     /locus_tag="AC161X_09005"
     CDS             9910..12600
                     /locus_tag="AC161X_09005"
                     /inference="COORDINATES: protein motif:HMM:NF012321.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HIR4989732.1"
                     /translation="MTFDHPWALLLLIPAVAVVLLIARHGRTVVGRRQSRWAAAVRVV
                     GVTALCVAIAQPHMILQSGDQTVVFLLDRSDSISEVAATTQEQVLREALQSVPASARW
                     SVVLFGAETRTDRSLAVGDRLPEIVTKVDGSATDLGGALRAVAALMPSAGSRRIVLFS
                     DMAPTDSEGRQAARELVEQGIVVDVIELSVGRRPDALVESVRLPTSVRQGDVVTATAH
                     VRSNSAGEAVLRVTGIEEQAREIVVQLQPGSSTIEFDFVANGTGFLPITTTLDAPFDQ
                     MPQNDTATGLTRVLGPARVALVEGVVGEGGELSRALAAGGVRVDILGSIPSATGLLPY
                     DAVVLVNIPPPESGPAEALASFVEDLGRGLIVVGGDQSFGMGDYHQTPLEVILPVSSN
                     PDDLLRRQPVAEVLVIDTSGSMAACHCDGSAANMATEGGINKTDISKAGAALAIEALD
                     DMDRVGVLSFSSGYDWVLPLANKPDKATIDAALGSLFPQGDTEISRALEEALDQLRGA
                     PESLRHIVLFTDGWDPTDADLVPVAREIADEGVTLSVLGTGEGPGHTLERMAEVGGGR
                     YYAGTDLAAVPDIFVEETLTVARNLINEGSFLPTVIGRSDVTESLTESPPLLGYVLTK
                     AKGSAQVLLEIGQADPLLATWQRGLGRVAAWTSDATSRWSSGWVGWDGYSDFWGGVLR
                     DVLPAGLDTPPEVWVDGTEINVAVFVDDLADDSAAVARIRSPDGSIATEPLTRATGSS
                     FEGSFPAGSPGAYWVAVTITDEQGATETVSSGTVSSYEPEFAFREPDPTLALDISELT
                     GGRIDPDASTIWDSAPLRGTSARPLLPWLLTLALVAFLTDVALRRLSFTPAESSVPKS
                     PSPPRRKRDPVERPEPTADRPAPTVSDSETIQRLMRRKQR"
     gene            12597..12743
                     /locus_tag="AC161X_09010"
     CDS             12597..12743
                     /locus_tag="AC161X_09010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989733.1"
                     /translation="MTAVTERRQVLAVYARRRLIADQVDVQEDGSESRGQLLERLSDA
                     MDVR"
     gene            complement(12804..12877)
                     /locus_tag="AC161X_09015"
     tRNA            complement(12804..12877)
                     /locus_tag="AC161X_09015"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(12841..12843),aa:Pro,seq:cgg)
     gene            13003..13323
                     /locus_tag="AC161X_09020"
     CDS             13003..13323
                     /locus_tag="AC161X_09020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989734.1"
                     /translation="MAVYDAQLRLGGANDAPIHVVIDLTDDRLSMMSGEVEVADWARH
                     EIRVTPMLDGFHIRAEGDEIILNVTDDAHFALDLGLRNAPPTLRRRMSALLRSDQSET
                     RPVA"
     gene            complement(13320..>13859)
                     /locus_tag="AC161X_09025"
     CDS             complement(13320..>13859)
                     /locus_tag="AC161X_09025"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF014793.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR4989735.1"
                     /translation="SGSGQQGVSELIDQVDKLRPDLGALMTGGWADPGPALFSRPIAF
                     NVLPFAGSETDQGYTDEEWKLVHESRKILDIPGLLVEPTCVRVPVLVGHAVVATLRFD
                     REMGPGEARELLEAAPGIEVWTDQVPTPLDAAGIDEVLAGRIRATVGEPGGINLFAVG
                     DNLRKGAALNAVQIAELLI"
BASE COUNT         2457 a         4344 c         4476 g         2582 t
ORIGIN      
        1 ccgagcttcg agtagatgag ctccatgacc atgcccagca cgacgaccac gaccaggacg
       61 gccatcaacc agaggaaatt ggcaaagtcc acggcgtctc caaatacgag atcgagcacc
      121 tccaggaaga tgaacttcga gaagaagagg atcgcccaga tgctaaagaa gtgggcgatc
      181 cgacgttcgg gccgactcga gtaccagacc ccaacccgcc tcttgaagcc aaaggtgacg
      241 taggtgagga cgtacaggac gagggaggcg agaaagatca aagtgaagga atcgatcgtg
      301 acaccgtcgg tgtactcgct gaagaaaccg aggacgacca gatagagcaa ggtgtcgagg
      361 gtccagtcga aaaagacccg ttgcttgttg ctcatcactt cctggaccac cctcgagaat
      421 gggcacgcta cccctgaaac gagatcagac cgctccggcg agcggcctga tcctgagtcg
      481 ggacggtggg acttgaaccc aagacctcag cgtcccgaag ggacccgggc cggattcgga
      541 cggactgtgg cggactaaaa ccgctggtca gactgtgtcg aggcgaagct gatgttccac
      601 gaggagtcaa cgttccacag cgcgggcacc ccagttgtgg acggcacgtt cggccgtgcc
      661 catagctgcg attttcgttg cctaccgcga cctattcaat agagtcgtcc tacctgcact
      721 tacgagggtg tgttcactca gggaacctaa ccgagggtgg cgacgtctga tggggacaat
      781 gtggaggttg gtgatgttcg ctgtcctggc gacggtctgc ctcccaatga gtgtcgctgg
      841 tgcccaacag gacgctgccc agctctccgc caccgtcgga gtcgaggggt gggtgtcggg
      901 cgacgagccc ttgacagtcc atgccacgat cgacgcagct ctgctcgtcg caggcttcct
      961 ccgtgtgtcg tatggcggtg cggtcactga aatcgaggtc gacgtcccag ccggcggatc
     1021 gaagacctac gacatcctcc tcaggccacc tttccgcaac gggtcggtgc aagtcattct
     1081 gttcgacgcc gatggacaac gtctgacttc tcagtcaatc aggccgcagg ttgccgacga
     1141 cgaggtgata gtcggcgttg cgggtgattc cgggttgaca acgaccctct ccgccctaca
     1201 gacgtcgatc gatgggctct cgatcacagc actcgacatc tcagagtctc ttccccgcgc
     1261 cgatctagaa ggtctctcct acctcgtcag tcgggatgtg gacccggtcg tctgggagtg
     1321 ggtgtccgat ggtgggcatc tggtgaccac gcgcggtgaa ctttccgaga cccctctttc
     1381 actcgagccg atcggcacgg tgccgggcac cgacgtggag tggtacacgg cgggacccac
     1441 aggtgaggtg tttctcatcg atggggtcgc agcctccgag gattggacga gagtgattcg
     1501 accccttccc ctgaagctga tacctaccga ccagtgggga tccaccgacg gttcgttggt
     1561 gcaggccgcc accaacagcg gagacgcagg attggcaaac ctgccgtggc tcccattcgc
     1621 catggtcgcc tatctcatct tgatcggtcc gatcaatttc atggtgctca agaggatgag
     1681 gcgacgtgag ctggcgtggg tcaccattcc ggctatggcc gcgattgccg tgctcggttt
     1741 ctgggcttcg gggcgtcagc gtctcgatgt gacgtcggcc cgacatgcca ctgtggtggt
     1801 ggctggagag caggcctatc aacggtccat cttcgtcctt gccgcaggca acgccggtgc
     1861 ctactcggtg gctgtgcctt ccgccgagcg gtatgccgtg acagacgcgt ttgcagctct
     1921 gggtggaaac gtcgggccga cctcggcggg gcggctatcg gcggatggcg tcgcttggca
     1981 gttgccgcag ctgggcgtgg cagccgtgga gacctggcag tcggcaaacg ggtccatcac
     2041 cgttgactca tctcttgatg gtgacacacc catcctcgcc gtcaccaatg acaccggact
     2101 cgacatcgag cattgggggg cggtgatcgg aggtgaggtc cacgttgctc agcaaggctt
     2161 ggcgaccggg cagtcagcga ccctgcgggc cggtggagcg gtagcagctt ggccgggtat
     2221 gtcctttgga gacgtgctcg tggagcagct tcaaatgtgg gacgacacgg gctggcaggt
     2281 catctcaccg atgggatacg ccgcacaagg ggagttctcc ccgagcgact cctacgtctt
     2341 cggtatatcg atgtcgacac cgatggaggc gatcgtgaat ggctcggccc agatggtcga
     2401 tggtcccact gtatggacga cgcccttttc gacacagggc ttcccgagtg ggaagactat
     2461 gggccgtgtt gtcggcgtcg gggactggcg ccaaatcgag gcatctcagg gcaatctctg
     2521 gctcgagacc gaccggatcg tcacgagctt cgaccttccc accgacgcca caaacaccag
     2581 gctgggggtg gccgccaatt tcggggccat cggaaagttg gagatgtgga actggtcgac
     2641 cggcgccttc gatgcggtcg agctcggcca ggagttcgat ccggtgagct accgatctgc
     2701 gtcgggcgag gtcatcgtcc ggatatttgc ggcaaacaac ggggagccgc cctatccaca
     2761 atcagtctcc gtggaatgga caaggggcac atgatcgagt tcgagtcggt cgagaagcgc
     2821 tttgggcaag cgaaggctct cgatgggttc gatctttcag tgcccgaagg cgcaatcctc
     2881 gggctcatcg ggcccaacgg cgcgggtaag acaacctcga tgcaagtgat gagcacattg
     2941 ctcgaacgtg atggtgggcg ggtgcgagta gccggcgtcg atccggccga cgacgctgcc
     3001 tcggtccgca ggattttggg ttacatgccg gacttcttcg gcttctacga gtcgctgacg
     3061 gcgtacgagt atctcgagtt cttcgcagcc agccagagaa tcgctccttc cgcgcgttcg
     3121 gggctcatct ccgacctgtt ggcgttggtc gatctcacat cgaaagccac cgccgacgtc
     3181 aacactttgt cacgggggat gaaacagcga ctctccctgg cccgctcact actcaatgac
     3241 ccgcagttgt tgatcctcga cgagcccgcg tcggggctgg atccgcgggc acgagtccat
     3301 cttcgagagc tgatcgccga gctctcccga atgggcaaga cggtggtgat ctcgtcgcac
     3361 atcctctcgg agctggaggg catctgctct cacatggcca ttgtcgaccg gggcaaggtg
     3421 gtagcgcaag gtcttctcga agacatccgg ctgcttcttg tggcgtcgcg cagggtgact
     3481 gcgcgcatgt ccgatgacgt tatcgacacg gtggaggatc ttctgcgtgc ccgcccggag
     3541 gtatccgaca tggcggtgga acgaggcatc attcgattcg tcctcgctgg cgacagggcg
     3601 gagtcggcag ccaccctgcg ctatatcgtc gagtccgggg tcgacgtttc cgaatggcgg
     3661 gtcgacgatg cgggcctcga ggaactgttc atgcaactga ctgaggatgg tgacgtgtga
     3721 acctgaatcc agtcctcgcc agagaagcca agcaacggtt ccgctcgaag cgcgtcgtgg
     3781 tggttaccac cctgtgggtc ggcatcatcg ggggaatcgc attcctgttg tacatcgccg
     3841 caagcgaggt ggcggtcaac gggatgggag ttggccggct cgcggccgcc gccttcatgg
     3901 gacgcttcat gttccactcg atcacactgc tgctgctcac ggcgatcatc ctggtggtgc
     3961 ccggaatcgc ggcaccgtcc atcatcggtg agcgagagcg acagacgttc cagatcctcc
     4021 aggtgacaca gatgactcct tggcagctgg tgacgggaaa actgacggcc tcgatgatgt
     4081 tcgcagtggt gctcctgttc gcagtcgccc ctgtcgcggc gattcccctg ctgttcggtg
     4141 gcacgtcgct cacggatgtc ctggcggctc tgggcatgct gttactcacg gccgtcaccc
     4201 ttgcctcgac agcgacctgg atgtcatccc gagccgcatc gacacgaggc gctgttggca
     4261 tgtcctatct cattgctttc acgctcggct ttctcacctt cgtcggactg ggtgccgaaa
     4321 tgctggtgta ttcgaatctc gggagtcgag atccgatggg tcgagatggc cgggaggtgt
     4381 tttcgatcct ccccaacccg tacttcggcc tggtcgacgc cgtgctgcat ccccttgacc
     4441 aaactgcctc gacgtcggac acgccatacc tgccattcga gtacatgttg aggttgcgtc
     4501 agggcgtcaa cccagacctc gcggagccgg cgatcttagg tggcgttgct gcgggcaacg
     4561 ccgcggggcg cgatttcccc cgacctccgc tgtgggcctt caacgtcgtg atatacgggg
     4621 gtctgaccgt cttctccttg cggcgggcca gcgtcaacgt ccgcgccccg tcggcgaaga
     4681 tccgcaggcc gaaacggctc aagggcacgg atgcttgaca cggcgatcga tcacctgatc
     4741 agcggggcca ggcgtcggat ttggctcagt aggaccgtgt cgttggcaac agccgctatc
     4801 ggggtcggcg ccacgatgat ggcgatgctt gcggcggccg cccacctgtg ggtgttgcca
     4861 tgggccgagc ctgcgggtct ggtcgtggtc ggtggatgtc tcatcacgtt gctcgttgtg
     4921 accttcctcg tccgtcccgg accccgtgag accgcccaga tggtcgatcg ttcgctcgaa
     4981 ggattcgacc gtgtcaccac ggcctacgac ctgaggcgat cggggccggt cgctgtgatg
     5041 gaacggaggc aagtcgaggc cgccgatcgc tgggcagacg gtcggcgacc cgatcctgta
     5101 gcgacattga tgccggcgcg ccgcctcctg gtcgcagctg cggttgccgt gatgggtgct
     5161 gccgtcctcg ggctcattcc atcgatcacc gatcgggcgc tggcccaacg tgaggcggca
     5221 cgggccgcca tcgcggtcga agcggatcgt ctcgaggatc tggccgagtt ggcaccggag
     5281 gagatcgccg ccgagcttca ggtcctcgca gacgaccttc gtgagctcgg ttcgatcgat
     5341 gatgcgctcg cccgcctcga agaggcaagg agggacctgg cagacaattt ggagccgacc
     5401 gacctcgctg agaggacggc cctcgcgggc ctgcagcgaa gcctcgaaca atccggtgct
     5461 tccgatggcg gggattcgcc ggccgagacc ttgggacaac tggcagcagg cgcaggcgag
     5521 ctgactccgg ccgaagccga gcaattggcc tcggagctct ccgaacgtgc gggggatttt
     5581 gcgggggtgg acaacgaagt ggcagagtcg ctggccgcgg cagcgaccgc tctctccggc
     5641 caggcggccg gtcgctcggg gtcggcaaac agcgaatcag ccctgagggc tgctgccgac
     5701 gcagtcgctg cctccgaatc ggcttcgacg gcggccgccg catcacaggc tggtgcggcg
     5761 gcagcagcag aggcacaaca acgtctccag caagggcaag ggcacgggca agggcaagga
     5821 caaggacacg gacaagggca agggcacgga caaggacaag gacaaggaca cggacaagga
     5881 caaggacaag gacccggcgg agggggaggc ggtagcggtg gtcaaggcca gggaggcagc
     5941 tctcagggag gcacagggtc cggttcgcta gatggcgcag gaggcgacca ggatcctttc
     6001 agcgcgccgg aacgagcgac agtcttcgat ccagtcgtct cagggcttgg ggaggaagtt
     6061 cgagtcgaca tcgagggtga gggcgacgga gagacccagg gcaccgtcga tggtcaaggg
     6121 atcgaaaacc tgcctctcat cccctacgcc gaccgattcg ccgaataccg gcagaccgcg
     6181 ctcgatgccc tgagtaccag cggaatcccg gcacagtctc aagatctggt gcgcgactac
     6241 ttcttggagc tcgaaccatg acgacatctg ccaggtccga tgaactgact cccgaagtct
     6301 tcgcccaacg tctcgccgca gtccgcaacg aggtcgagaa ggtgatcgtg ggtcaagccg
     6361 atcttgtcga cacagtgatg atggccacgc tgtgcgaggg gcacgtcttg ttggaaggcg
     6421 tcccaggcct cggcaagaca cgcctgctcg caacgctggg gcaagccctc gatgtcgagt
     6481 tcgggcgcat ccagttcaca ccggacctca tgccggcaga catccttggg accaaggtcc
     6541 tcgagcgggg tgatttcgag ttctcgccgg ggccgatttt caacaacctg gtcctcgccg
     6601 acgagatcaa ccgggccact ccgaagaccc agtctgccct cctggaggcc atgcaggaac
     6661 ggaatgtcac gctcggtcgc gaggtgcatc ccttgccacg acctttcatg gtcatggcaa
     6721 ctcagaaccc aatcgagcag gaagggacct atcccctgcc ggaagcacag ctcgatcgat
     6781 tcctcttcaa ggcgatcgtg tcgtttccac ctgccaccga tctcgtcgag atcctccgcc
     6841 gcacgaccgc caccacagat ccgtctgccg aggccgtgct cgacgggccg cgcctcgagg
     6901 aaatgacccg gctggtgagg tcagtgccga tcggcatcca cctgctggaa tatgctgctc
     6961 ggctcgtcga gtccacccac ccggcgagcc ccctggcgcc ggagcgcatc aaggcctacg
     7021 tccgcaacgg ggcaagcccc cgcggcgccc aggccatgat tctcggcgga aaggccgcgg
     7081 cgctcatggc cggccgcccc aatgttgccg ccgaggacct gtggcggata gcgggccagt
     7141 gcctgcgtca tcgccttgtg ctcggctacg aggccatcgc cgacggtgtt tcggcggatg
     7201 cgctgatcga cgacgtgctc caggcgcacc ccgcaccctc ggtcgaggac tgacccatgc
     7261 tgctggcgcc ggagacgcgg agccgcctcg atcggctggc tctgtctcct cggggccgtg
     7321 ttcaggctct ctggtcggga ggtcacgcat cggtgcgcaa aggggagagc ctcgatttcg
     7381 ccgactatcg cccgtatgtc ccaggagacg acttcaggaa gatcgaccac aacctgtggg
     7441 cgcgtctcgg acaaatcctg atccgtgagt acgaggccga ggaggagctc cctctgcgtc
     7501 tcgccgtcga ctcgtctgcc tcgatgtcgc tccatggaaa acacgagacc gctctcgagc
     7561 tcgccgcgat gatcgcctat ctcacgctga gcggagggga ccgggttcag cccgtgtcct
     7621 caccaggtcc aacgggtcgg gcgttcgccg ttggtccctc tgggcgtcac ctgagtgtct
     7681 ggccggtcat cgagcgatgg ctcgagagcc tggaggcgtc tggtcgcgca ccgcttggcc
     7741 cggccattcg ctcgctgatc ggcggaagcc caacccgcgg accggtcgtg ctgatctcgg
     7801 atctcttcga cgatgagtgg gagcgggcac tcgactcgct cttcgtcagt gcaggcgggc
     7861 tcgtgttgca tgtgctggcg agagaggagc tgtctccgga cttcgccggc gacttgaggt
     7921 tgcgcgacat agagctgggc tccgtggtcg acatctcgac gtcggaggcg gtgttgacca
     7981 cctatcgagc gtctctcgat gagttcgtcg ccggcgcagc aaatcgcgct cgccgcgccg
     8041 gtctcgatta cctgttggtc gaagccggtt ccgatgctgc tgagaccgcc ctcagggctc
     8101 tcttgtcagc cggagtagtt cgatgagctt tctcgtcccc gtcgctctcg gcgtcgctgc
     8161 gctagccggg ccccttctta tcctgtacat gcttcgcagc cggagacgac aactcgatgt
     8221 cacttcgaca cggttgtggc aggcgagtga aattcaggtc acgtcggcag tgccatggtc
     8281 ccgtctccgg tggactgcac tcctcattct ccagcttctg gccatagcgc ttttcgcctt
     8341 cgccctggct cggccattcc acagccagcg gaccctgctc ggcccgcact cggtgttcgt
     8401 gatcgacacc tcgggatcca tggcggaggg cgaccggctc gaccgagcca agacggaagc
     8461 agtcgccctc gccgaagact tgtccgaagg caatctcatc tcggtgatcg gagccggacc
     8521 tcatccccag gttctgatcg gacatgcccg cgaaagggac gacgtgatcg ccacgatcga
     8581 gagcctgcgg gccggcggcg gcgaagcgga cctctccgag gccatcaggc tgggccgcgg
     8641 attggccaca cccgaccggc ccaccaacgt catcgtcttc tcagacggtg gtgaggcgcc
     8701 gttacccgag gagcccgtgg cgggtgccac gcatatcccg ttccacgaaa tcgtggacga
     8761 cgtagccgtt gagactctca ccatcgacgc cacgggaggt gtcacgcgag gtctcgtcgc
     8821 cgtagcgaat cactccgaag ccgatacgag cgtcgaactc gccgtgacgg tcgaaggcat
     8881 gccctcaacg cagatggagt tggacctcgg tgccaaccag cgcgcagtag agaccttcga
     8941 gctcgatgcg gccccaggat ctgaggtgat ggtggagcgc ctgggtgagg cggacggcaa
     9001 tccgctggac gatcgggtgt ggactgtcat cgatcagaac gcagcccgct ctgtgcgagt
     9061 catgggagaa ggctctccat acctcgccgc cctggtgtcg gccaccccgg ggttcgaact
     9121 cggctccgac gatggcgacg tcctggtggt cgaccgcggg gtgctacccc cgatcgatcg
     9181 gcccgcctgg ctgatccgtc cggattcgac gccggaggga ctcgagatca cgggtctcgt
     9241 ggagaacatt cccgcgacgt tccaggctcc tggagagccg gtgcttgaca atgtggacct
     9301 cagcgacctg gtcgttgcag aagcccagaa cgtcgcgccg agaggctggt cgcccatcgt
     9361 caggtcaggc gacgtgccac tcatcctgct tggcagcgtc gatggtcatc gcgtcgtcta
     9421 cttcacgttc gatctcaccc acacgaacct gccggtccag gtctcgtttc ccattctggg
     9481 agctgccctc ctcaactggc tggtcggcga ggcgccgggc accgttgcca atggcccggc
     9541 gggcgagccg atcgtcattt catcgcccga tggagcccaa cccgtcgtca ccttgcccga
     9601 tggctcccag cgaacgttgc cgggcgatac cgcgagtttc accgatacga gtgagccagg
     9661 catctaccgc gtcggctacg tggacgagga cggcacagtg acagcgggcc agatcgctgt
     9721 gcggactttc gtcgcgaccg aggccggcgc tccggtgaga gacatcgtca cctcaacagg
     9781 agattccagc gcagaggagc aaggagtagt ggtccgggag tggggaccgt gggtcgttag
     9841 tgccctcctg gccctgctcg ccttcgaatg gtggatcggg catcagcggc cctggctgag
     9901 gcagacagca tgactttcga tcacccctgg gcgttgttgc tcttgatacc tgcggtcgcg
     9961 gtcgtgttgc taatcgctcg tcacggacga accgtggttg gtcgcaggca atcacggtgg
    10021 gccgcggccg tgcgggtagt gggcgtgact gccttatgcg tcgctatcgc acaacctcac
    10081 atgatccttc agtcagggga tcagacagtg gtcttcctcc tcgatcggtc cgattcgatc
    10141 tccgaggtcg cggccacgac ccaggagcag gtgctccggg aggccctgca gtccgtgccg
    10201 gcctcagctc gctggtcggt tgtcctcttt ggcgccgaaa cgcgcacgga ccgatcgttg
    10261 gcggtcggtg acaggttgcc tgagatcgtc accaaagtgg atggctcggc gacggacctc
    10321 ggaggagccc ttcgcgccgt cgctgcgctt atgccatccg ccggctcgcg gcgcatcgtt
    10381 ctgttctccg acatggcccc gaccgactct gagggacggc aggccgcgcg cgaactcgtg
    10441 gaacagggga tcgtcgtgga cgtgattgag ctatcagttg gccgccgccc agacgcgctc
    10501 gtcgagtccg tccggcttcc gacgtcggtg aggcaagggg atgtcgtcac ggctacggcc
    10561 cacgtgagat cgaacagcgc cggtgaggcg gtcctacgcg tcaccggaat cgaggagcag
    10621 gctcgggaga tcgtcgttca acttcaacca ggctcgtcga cgatcgagtt cgatttcgtg
    10681 gcaaacggaa cgggattcct ccctatcacg acgaccctcg acgccccgtt tgaccaaatg
    10741 ccacagaacg acaccgccac cggtctcaca cgcgtgctgg gaccggccag agttgctctc
    10801 gtcgagggag ttgtcggcga gggtggagag ctcagtaggg ccctggccgc gggcggagtt
    10861 cgggtggata tcctcggctc gattccatcg gcgaccggtc tgctccccta tgacgcggta
    10921 gtgctggtca acatccctcc cccggagtcg gggcccgctg aggccctcgc atcgttcgtc
    10981 gaggacctgg gacggggtct catcgtcgtt ggcggcgatc agtcgttcgg catgggcgac
    11041 taccaccaga caccactcga ggtgatcctt ccagtgtcct ccaaccccga cgatctccta
    11101 cgtcgtcagc cggtcgccga ggtgctggtc atcgacacgt cggggtcgat ggccgcctgc
    11161 cactgcgacg gatcggcggc caacatggcc accgaaggcg ggatcaacaa gacagacata
    11221 tcgaaggctg gcgctgcatt ggccatcgag gcattggacg acatggatcg agtgggtgtc
    11281 ctttccttct cctccggcta cgactgggtg ctcccccttg ccaacaaacc ggacaaggcg
    11341 accatcgacg cggcactcgg cagcctgttc ccccaaggtg acaccgagat cagcagggct
    11401 ctggaggaag ccctcgacca actacgcggg gcacctgaga gcctccggca catcgtcttg
    11461 ttcaccgacg gttgggaccc gaccgacgcc gacttggtcc cggttgcccg cgagattgca
    11521 gacgaaggcg tcactctttc ggttctcggg accggagaag gaccgggaca cacactggag
    11581 cgcatggccg aggtcggcgg tggccggtat tacgccggga ccgacctggc agcggtgccg
    11641 gatatcttcg tcgaggagac actgaccgtg gcccgcaatc tgatcaatga gggctctttc
    11701 ctgcccacag tgatcggtcg gagcgacgtt acggagtctc tcaccgagtc ccctccccta
    11761 ctcggctacg tcctcaccaa ggccaaaggg tcggcgcaag ttctgctcga gatcggtcaa
    11821 gccgatccgc tcctcgccac ctggcagcgc gggttggggc gcgtggcggc gtggacgtcc
    11881 gatgccactt ctcggtggtc ttcaggatgg gtcggatggg atggttactc cgacttctgg
    11941 ggaggagtcc tgcgcgatgt gctcccggcg gggcttgaca caccacccga agtgtgggtg
    12001 gacggaactg agatcaacgt agccgttttc gtcgatgacc tcgccgacga ctccgcagcc
    12061 gtggcaagga tcagaagccc agatggatcc atcgcaacgg aaccgctgac ccgggcgacc
    12121 ggttcatcct ttgaaggctc ctttccggcc gggtcacccg gggcatattg ggtagcagtg
    12181 actatcaccg acgagcaagg cgccacagag accgtctcgt caggcaccgt ttcgagttat
    12241 gagcccgagt tcgcattccg ggagcccgac ccgaccctcg ccctcgacat atccgaattg
    12301 accgggggca ggatcgatcc cgatgcgtcc accatctggg actctgcacc gctccgaggg
    12361 acatcggctc gtccgctcct cccctggctg ctgacgctag ctctggtggc gttcctcacc
    12421 gacgtggcgc tacggaggtt gtccttcaca cccgccgagt cgagcgtgcc gaagtcacca
    12481 agtcctccgc gccgcaaacg cgaccccgtc gaacgtcccg aaccaacggc cgataggccg
    12541 gcacccacgg tgtcagattc ggagacgatt caacgcctga tgcgtcgtaa gcagcgatga
    12601 cagcagtcac ggagcggaga caagttctgg cggtctacgc gcgtcgtcgc cttatcgcag
    12661 accaggtgga cgttcaggag gacggctccg aatctcgagg acagttgttg gaaagacttt
    12721 ccgatgcgat ggatgtgcga tgagctccgg ctcaacgaat cagggccgcc ctctcgaacg
    12781 gccctgaact gagggtctct tagtcgggac ggtgggactt gaacccacga cctcagcgtc
    12841 ccgaacgctg cgcgctacca agctgcgcta cgtcccgatg gccaaatcct aggccggagg
    12901 cacggtctag cccacttggg tgaattcagg catccccctg ggagattccg gcctggcagg
    12961 tacgctgacc aaggaggctt ctgtaagcga tggggaggaa tgatggcggt ctacgacgcc
    13021 cagcttcgtc tgggcggcgc caacgatgca ccgattcatg tggtgatcga tctcaccgat
    13081 gatcgattga gcatgatgtc gggtgaggtc gaagtggccg actgggcccg gcatgagatc
    13141 agggtgacgc ccatgctcga cgggttccac attcgcgccg agggtgacga gatcattctc
    13201 aacgtcaccg acgacgccca cttcgctctc gacctcggtc ttcgcaatgc gccgccgacc
    13261 ctgcggcgtc ggatgtcggc gttgcttcgc agcgaccagt ccgagacccg gcccgtcgcc
    13321 tagatcaaca actctgcgat ctggaccgcg ttcagtgctg ctcccttgcg caggttgtcg
    13381 cccaccgcga agaggttgat cccacccggc tcaccgactg tggcgcgaat cctccctgcc
    13441 agcacctcat cgataccagc ggcgtccagc ggtgtcggca cctggtcagt ccagacttcg
    13501 attccggggg cagcttcgag gagctcccgg gcctccccag ggcccatctc tcggtcgaaa
    13561 cggagtgtgg ccactacggc gtggcccacc agcaccggca cccggacaca ggttggctcc
    13621 actagtagcc ctgggatgtc gaggatcttc cgcgactcgt gcaccaactt ccactcctca
    13681 tccgtgtagc cctggtcggt ctccgagccg gcaaatggca gcacgttgaa ggcgatgggg
    13741 cgggagaaga gggcgggtcc ggggtccgcc cacccgcctg tcatcagtgc accgaggtcg
    13801 ggccggagct tgtccacctg gtcgatcagc tcggaaacac cctgttggcc cgaaccgga
//
LOCUS       DBNHXU010000367         3116 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3917837, whole genome shotgun sequence.
ACCESSION   DBNHXU010000367 DBNHXU010000000
VERSION     DBNHXU010000367.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3116)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3116)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3116
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3917837"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..793)
                     /locus_tag="AC161X_09030"
     CDS             complement(<1..793)
                     /locus_tag="AC161X_09030"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: protein motif:HMM:NF012509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HIR4989736.1"
                     /translation="MSVFEAPAKVNLSLHVEPPATNGYHPITSLVQTIEWCDRLEVFE
                     GEDDEITVVGADLDTEDNLVHKAIEAVRLRRAVPPLRIRLRKRIPIGSGLGGGSSDAA
                     AALLAAFSFGAGDETEMSEIAASIGADVPLFLVGGTILMSGFGEVIDPQPALPRLSIA
                     VAVPEFSFSTDDVYRKWDEMEGPIGETIGSGSLPPQLRGGMPIRNDLTPAALELEPAL
                     GDFIADLRSVWGTALMTGSGSGCFGFFSDPEEATDAADTVRKWSRV"
     gene            complement(790..1593)
                     /gene="rsmA"
                     /locus_tag="AC161X_09035"
     CDS             complement(790..1593)
                     /gene="rsmA"
                     /locus_tag="AC161X_09035"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: protein motif:HMM:TIGR00755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0000154 - rRNA modification [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HIR4989737.1"
                     /translation="MKAQTRSEIVDLLERHGLTPTHHLGQNFLADSNITRKIVDLAGL
                     HPGDRVVEVGAGTGTLTRALASTGSHVVAYEVDSKLAPVLEEVTAGLDVELRFADVMD
                     VDLGGALGPGRWVMVANLPYNVGTPLVLDALRSVPDIVRFVVMVQREVAERFAASPGG
                     KEYGLPSVICGIYAPARVAFRVPPQVFYPAPSVESAVVIMDRVPAPPDADRAVELAGA
                     GFNQRRKMLRRSLDQMFEDPTASIRAAGLDPTARAEDLAPADYLRLARV"
     gene            complement(1590..2336)
                     /locus_tag="AC161X_09040"
     CDS             complement(1590..2336)
                     /locus_tag="AC161X_09040"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004536 - DNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HIR4989738.1"
                     /translation="MGWVDTHCHLQLDGRDPAQLLERATDIDWVVAPGVDLESSEASR
                     ALASRFPGRVLATAGLHPHDAESWPAQGDGIARLAGEAAAIGETGLDFYRNLAPRDDQ
                     VTAFREQIALAANLSKPIIIHCRDAFAEVFEIVEQTGIGGQSVLHCWTGGPKWTKRFL
                     TLGVMFSFAGPVAFLTGDTVRLGAAEVPPERAMVETDTPYLAPPPHRGEPNEPAWVAF
                     VGAALAEVWDMNVDEVARITSANATRVFKA"
     gene            2407..2643
                     /locus_tag="AC161X_09045"
     CDS             2407..2643
                     /locus_tag="AC161X_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014116412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HIR4989739.1"
                     /translation="METGIARKIDDLGRIVIPAETRRLFNIREGDHLFIAVEGSSIVL
                     RKMTDTCTFCGSDTKVTQFKGRGVCSSCRSQLRS"
     gene            complement(2628..>3116)
                     /locus_tag="AC161X_09050"
     CDS             complement(2628..>3116)
                     /locus_tag="AC161X_09050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HIR4989740.1"
                     /translation="VTSAVAVAGFGGEGFVFEGFLPRRGKARSQRLQRIAVEDRPVVL
                     FLAPHRFEADLVDLASTSGGERRLVVARELTKLHEEVWAGTINEALDEWSQRTPKGEF
                     TIVLAPAEPPVVSLSEAVEQARSLVSGGWTRSEASREVGRATGLPRRQIYEALLEPQD
                     RS"
BASE COUNT          580 a         1025 c          999 g          512 t
ORIGIN      
        1 ccacccgcga ccacttgcgg accgtgtcgg cggcgtcggt ggcctcttcc ggatcggaaa
       61 agaatccaaa gcatcctgac ccggacccgg tcatcagggc tgtgccccag accgagcgga
      121 ggtcggcgat gaagtccccc agcgccggct cgagttccag agccgccgga gtcaggtcgt
      181 tgcggatcgg catgccaccg cgcaattggg gaggcagcga cccggagccg atcgtctccc
      241 caatcggccc ttccatctca tcccacttcc ggtagacgtc gtcggtcgag aacgagaact
      301 ccggcacggc gaccgcgatc gacagcctgg ggagggccgg ttggggatca atgacctcgc
      361 caaagcctga catcagaatt gtgcccccca ccaggaacag gggcacgtct gcccctattg
      421 aggcggcgat ttcggacatc tccgtctcgt cgccggcacc gaaagagaag gccgcgagca
      481 gcgctgccgc tgcatcggag ctgccgcctc caagacctga tccgatcggt atccgcttcc
      541 ggagccgaat ccgcaacggt gggactgccc gacgcagtcg caccgcctcg atcgccttgt
      601 gcaccaggtt gtcctcagtg tcgaggtcgg cgccgaccac ggtgatctcg tcatcctccc
      661 cttcgaagac ctcgaggcgg tcacaccatt caatcgtctg caccagagag gtgatcgggt
      721 ggtagccgtt agtggcagga ggctccacat gaagagacag attcaccttg gctggggcct
      781 cgaacacgct catacccggg ccaacctcaa gtagtcggct ggcgcgaggt cctcggctcg
      841 agcggtgggg tccaggccgg cggctcgaat cgaggcggtg ggatcttcga acatctgatc
      901 aagggacctt ctcaacatct tgcgacgctg attgaagcct gccccggcca gctcgactgc
      961 acgatcggcg tcgggcgggg cgggcacccg atccatgatc accaccgccg actcgaccga
     1021 aggcgcggga tagaacactt ggggaggcac tcgaaatgcg actctggccg gcgcgtagat
     1081 gccgcagatc actgaaggaa gcccgtattc ctttcctccc ggggaggcag cgaaccgctc
     1141 agccacctct cgctgaacca tgacgacgaa gcgaacgatg tcaggcacac tgcgcaaggc
     1201 atcgagaacc aacggggtgc cgacgttgta ggggagattg gcgaccatca cccatcgtcc
     1261 agggcccaat gcgccgccga ggtccacgtc catgacgtcc gcgaaccgga gctcgacatc
     1321 gaggcccgcg gttacctcct cgagaaccgg cgccagcttg gagtcgactt cgtaggcgac
     1381 gacatgcgag ccagtggatg ccagagcacg agtcagagtg ccagtcccgg cgccgacttc
     1441 cacaacccgg tcgcccgggt ggaggccggc aaggtcgacg atcttgcgag tgatgttgga
     1501 gtcggcgagg aagttctgac cgaggtggtg tgtcggtgtc agaccgtgtc gctcgaggag
     1561 atcgacgatc tcggacctgg tctgcgcctt catgctttga acacccgcgt cgcattggcc
     1621 gacgtgatcc gcgccacctc gtcgacgttc atgtcccaga cctcggcgag cgcagcaccg
     1681 acgaaggcga cccacgccgg ctcgttgggc tctcctcgat gcgggggcgg ggcgaggtag
     1741 ggggtgtcgg tttcgaccat cgcccgttcc ggcgggacct cggctgcccc caatcgaacg
     1801 gtgtcccctg tcaggaacgc gaccggcccg gcaaacgaga acatcacccc cagggtcaga
     1861 aatcgcttgg tccacttggg gccacctgtc cagcaatgga ggaccgactg accaccaata
     1921 ccggtctgct cgacgatctc gaagacctcg gcgaaggcgt cccgacagtg gatgatgatc
     1981 ggcttgctca agttggcagc cagagcgatc tgctcgcgaa acgccgtgac ctggtcgtca
     2041 cgcggggcca ggttccgata gaagtcgagc ccggtctccc caatggcagc ggcttcgccg
     2101 gccagccggg caatgccgtc gccttgtgca ggccaggact cggcgtcgtg gggatggagt
     2161 cctgcagtgg ccaagacccg accggggaac ctcgaggcca gggcccggga cgcctcagag
     2221 ctttcgagat ccactccggg agccaccacc cagtcgatgt cggtggcgcg ttccaggagc
     2281 tgggccgggt cgcgcccgtc cagctggaga tgacagtgag tgtcgaccca tcccatcggt
     2341 gcggacggta cccgccaccg atcgcgggcc ccggggtagg ctcggatccc ggaggacaat
     2401 gcccgcatgg aaactggaat cgcccgcaag atcgacgacc tgggaaggat cgtgatcccc
     2461 gctgagaccc gtcgtctgtt caatatcagg gagggcgacc atttgttcat cgcggtagag
     2521 ggaagttcga tcgtgctccg gaagatgacc gacacctgca ccttctgcgg atcggacacc
     2581 aaagtcaccc agttcaaggg tcgtggggtc tgctcgtcgt gccggtctca gcttcggtcc
     2641 tgaggctcga gcagagcctc gtagatctgg cgacggggca ggcccgtggc ccggccgacc
     2701 tcacgggacg cctccgagcg cgtccaacct cctgacacca gcgatcgagc ctgctcgacc
     2761 gcctcgctca ggctgaccac cggtggctcg gctggggcga gaacgatggt gaactcgccc
     2821 ttcggtgtgc gctgtgacca ttcatcgagt gcttcgttga tggtcccggc ccacacttcc
     2881 tcgtgaagct tggtgagctc tctggcgacg accagccgac gttccccccc ggacgtagaa
     2941 gcgagatcca cgagatcggc ctcgaaccgg tgcggggcta ggaacaggac cacgggtcga
     3001 tcctcgacgg cgatcctctg gagacgctgc gacctggcct ttccccttcg ggggagaaaa
     3061 ccctcgaaca caaatccctc gccgccaaat ccggcgacgg cgacagcact ggtcac
//
LOCUS       DBNHXU010000368         3576 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4514802, whole genome shotgun sequence.
ACCESSION   DBNHXU010000368 DBNHXU010000000
VERSION     DBNHXU010000368.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3576)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3576
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4514802"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..673
                     /locus_tag="AC161X_09055"
     CDS             <1..673
                     /locus_tag="AC161X_09055"
                     /inference="COORDINATES: protein motif:HMM:NF012607.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003682 - chromatin binding [Evidence
                     IEA];
                     GO_function: GO:0003714 - transcription corepressor
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_function: GO:0140297 - DNA-binding transcription factor
                     binding [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C-terminal binding protein"
                     /protein_id="HIR4989741.1"
                     /translation="IVVTNVPDYSVEEVATHALALILGLIRKLNAADQKVRNGGWGLD
                     GLRPIRRLSTMTVGLVGYGRIGRRIAAPLQALGAGIIAYDPYLSPADDLPPLVSLEEL
                     LSRADLVSLHLPLTPETRGMIDESALAGMKPGAILVNTSRGPLVELEAVVQALESGHL
                     AGAGLDVFDIEPLDPVRIEGVPNLVATPHMAYYSEEALQESQRKATTQVIKVLTGEPP
                     DYRVN"
     gene            complement(684..1826)
                     /locus_tag="AC161X_09060"
     CDS             complement(684..1826)
                     /locus_tag="AC161X_09060"
                     /inference="COORDINATES: protein motif:HMM:NF012766.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HIR4989742.1"
                     /translation="MAKTHAALSDSQAGGLLVWKDENVRYLTSLRAQLIAGKTTSLNG
                     SLLLPGRDPILLGSGGEIDKARFGMSWIGEAHAVPIMEQRELVDGFVVDILAPILREA
                     GLDTGRLAVDQANISFINSMRSHLPDLELVDGDAVMQQARWIKSDLEVAIIEEACAIG
                     DSVTQRALDETRAGRREMDVAADAMQTLFQLGGEMAHVITPFVASGEHMSPPHRIATD
                     KIIRNQDLCFIDIGAMWNGYFADIGRTTIVGTPSKEQKKIYTAVYEGLMAGIDTMRPG
                     KTNQECADAIIAKIGEHGLEDHLFSLFIGHGIGMGANEPPYIGETLPGATTYEFQPNM
                     VFAVEPLVWVPNVRGGGGVRIEDMILVTNDDPHVLSRVEYEERLLT"
     gene            2128..3024
                     /locus_tag="AC161X_09065"
     CDS             2128..3024
                     /locus_tag="AC161X_09065"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR4989743.1"
                     /translation="MVGLLLLVVLVMGLNWPIMAVGTRHIPPIWLATFRVVGALLVVM
                     IGVTVSGRWRMPPRSDLPIIASLAIFRLAAVFVLVFTALALIPAGRSSVLAWTTSLWT
                     VPIAAVVLGEVMTGRRWFGLALGLVGIVVLVEPWNLNWSQSEVMWGHLFLVLAAVINA
                     ATSVHIRAHRWRMTPLQAIPWQLALAAVILGGVALATEGLPDISWTSQLVWIVVYQGV
                     VATGIAFWAEVVVFRSLPTVSTNLTLMGVPVVGVVASAVLLGEVVTASLVVGLVLVLV
                     GVAVNLLSGAQQIGEEAPVVTG"
     gene            complement(3038..>3576)
                     /locus_tag="AC161X_09070"
     CDS             complement(3038..>3576)
                     /locus_tag="AC161X_09070"
                     /inference="COORDINATES: protein motif:HMM:NF012335.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HIR4989744.1"
                     /translation="VVVIGAGGGVGSHMIQVARLHGAEVVGLDVGEQKLQSVADLGAR
                     PLSSASFSELSGRIGGRAPTLVIDLVGSAESIEWGRAALEPGGRQVLLTTYRGQSFPL
                     SPRQMVSSELTVLGSRYATRAEVATAARLVESGNVSPVIGRVVSPEEAPQVHIQLRRG
                     ALLGRGAIDWRSARDRAQ"
BASE COUNT          577 a         1102 c         1169 g          728 t
ORIGIN      
        1 catcgtggtg accaacgtcc ccgactactc ggtggaagaa gtcgcaaccc acgccctggc
       61 actcatcctc gggctgatcc ggaaattgaa cgccgccgac cagaaggtcc gcaacggggg
      121 gtggggcctg gacgggctgc ggccgattcg gagactgtca acgatgacgg tcggcctggt
      181 cggctacggg cggatcgggc gtcgcatcgc cgctccgctc caggcactcg gcgccgggat
      241 cattgcctac gacccctacc tgagcccggc ggacgacctc ccgccgctgg tcagtctcga
      301 agagctgttg tcgagggccg acctcgtgtc actgcatctg ccgctcacgc cggagacccg
      361 gggcatgatc gacgagtcgg cgttggccgg gatgaagccg ggggcgatct tggtcaacac
      421 ctcgcggggc cccttggtcg aactcgaggc agtggtgcaa gctttggagt caggccatct
      481 tgccggcgct ggcctggatg tcttcgacat cgagcccctg gatccggtgc gcatcgaagg
      541 ggttcccaac ctcgtagcca ctccccacat ggcgtactac tccgaggagg ctcttcaaga
      601 gtcccaacgc aaggcgacca cccaggtgat caaggtcctc accggggagc ctcccgacta
      661 tcgggtgaat tgaagaacga ccgctaggtg aggaggcgct cctcgtactc cacccgggac
      721 agcacgtggg ggtcgtcgtt ggtcaccaga atcatgtctt cgattctgac gccacctccc
      781 cctcggacgt tgggcaccca caccagtggc tcgaccgcga acaccatgtt cggctggaac
      841 tcataggtgg tggcgccggg gagggtctca cctatatatg ggggctcgtt ggcgcccatc
      901 ccgattccgt gcccgatgaa caggctgaag aggtggtcct cgagcccgtg ctcaccgatt
      961 ttggcgatga tcgcatcggc gcactcctga ttggtcttcc cggggcgcat ggtgtcgatc
     1021 cctgccatca agccctcgta gaccgccgtg tagatcttct tctgctcctt ggaaggcgtc
     1081 ccgacgatgg ttgtccgccc gatgtcggcg aagtagccgt tccacatcgc cccgatgtcg
     1141 atgaaacaga ggtcctggtt gcggatgatc ttgtcagtgg cgatccggtg cggtggcgac
     1201 atgtgttccc cggatgcgac aaatggcgtg atgacatggg ccatctcgcc tccgagctgg
     1261 aacagggtct gcatggcgtc ggcagcaacg tccatctcgc gacgcccggc cctggtctcg
     1321 tcgagtgctc gttgggtgac cgagtctccg atggcgcagg cctcttcgat gatcgccacc
     1381 tcgagatcgc tcttgatcca gcgcgcttgt tgcatcactg cgtctccgtc caccaactcg
     1441 aggtcgggga ggtgcgatcg catcgagttg atgaaggaga tgttcgcctg atccacggcg
     1501 agacggcctg tatcgagccc ggcctcgcgg aggatgggcg ccaggatgtc gaccacgaac
     1561 ccgtcgacca attcgcgctg ttccatgatc ggcaccgcgt gggcttcacc gatccagctc
     1621 atcccgaagc gtgccttgtc gatctcgccg cccgagccga ggaggatggg atcccgaccg
     1681 gggaggagca gggatccgtt gagagacgtg gtcttgccgg caatcagttg ggcccgtaac
     1741 gaggtgagat agcggacgtt ctcgtctttc cagacgagga ggccgccggc ctgggagtcg
     1801 gaaagggcgg cgtgggtctt cgccacccgt tctcgtcgga gcgcggcgaa gtcataacgg
     1861 tcttcccaat cgaccgcgaa cggccccttt gcgtaaccgc tgatcgttgt ctccctccat
     1921 gtatcgccac cgacagggcg gtggggggtc catggtcact ctatatagtc cgtagagtac
     1981 agaaagtttg ctgcatcccc aagaggctct gttggtgatc ctgccagcac caattccgct
     2041 tgattggccg gtgagcctag atcgcgagcg gctggcgtga tccccaggga ggatcggcga
     2101 agtcagaatg ggccgagccg tcgcgcattg gtcgggttgc ttcttctggt ggtcttggtg
     2161 atggggctca actggccgat catggccgtt ggaacccgtc atatcccgcc catctggctc
     2221 gccaccttca gagtggttgg agcgcttctg gtggtgatga ttggggtcac cgtttccggg
     2281 cggtggcgca tgcctcccag aagtgacctc ccgatcatcg cttctcttgc gatattccgt
     2341 ctcgcagcag tgttcgtcct cgtattcacc gccctggcgc tgatccccgc cggtcgatcc
     2401 tcggtgctgg catggaccac ctcactttgg accgtcccca tagctgcggt ggttctgggc
     2461 gaggtgatga ccgggcgccg atggttcggg ctggcgctgg gtcttgtcgg gattgtggtc
     2521 ctggttgagc cctggaacct caactggagt cagtcggagg tgatgtgggg gcaccttttc
     2581 ctggttctgg cggcggtgat caacgccgct acctcggtcc acattcgggc ccaccgttgg
     2641 agaatgactc ctcttcaggc gattccctgg caattggcgc tggccgccgt catcctcggg
     2701 ggagtggcgc tggcgaccga agggctcccc gacatttctt ggacgtctca gctggtctgg
     2761 atcgtggtct atcaaggtgt tgtggcgacg ggcatcgcgt tttgggcgga ggttgtcgtg
     2821 ttccgcagcc tccccaccgt gtcgaccaac ctgaccttga tgggggttcc ggtggtcggg
     2881 gtcgtcgcgt cggctgttct gctgggcgag gtggtgaccg catcactggt cgtgggactg
     2941 gtgttggtct tggtcggcgt tgccgtgaac ctgctttctg gagctcagca aataggcgag
     3001 gaagcgccgg tcgtcaccgg gtaggcgaac tcgagcgcta ttgggctcgg tctcgagcag
     3061 acctccagtc gatggctcct ctacccagaa gggccccacg acgaagctga atatggacct
     3121 ggggtgcttc ttcgggtgac acgacccgtc cgatcaccgg gctgacattg ccgctctcaa
     3181 ccagacgggc cgcggtggcg acttcggctc tcgttgcgta gcgcgagccg agaaccgtga
     3241 gctcactgga caccatctgc cttgggctca acgggaagga ctggccgcga taggtggtga
     3301 gcaggacttg gcggccgccg ggttcgagcg ctgcccggcc ccactcgatg ctctctgcag
     3361 agccgacaag gtcgatcacg agtgtgggag cccgaccccc gattcgcccg ctgagctcgg
     3421 agaaggaagc cgatgagagg ggtcgagcac cgaggtccgc gaccgactgc agcttttgct
     3481 cgccgacgtc tagtccgacg acttcggctc cgtggagccg cgccacctgg atcatgtgcg
     3541 atcccacacc gccaccggca ccgatgacga caacca
//
LOCUS       DBNHXU010000369         2896 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3323265, whole genome shotgun sequence.
ACCESSION   DBNHXU010000369 DBNHXU010000000
VERSION     DBNHXU010000369.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2896)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2896
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3323265"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..216)
                     /locus_tag="AC161X_09075"
     CDS             complement(<1..216)
                     /locus_tag="AC161X_09075"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:NF012439.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HIR4989745.1"
                     /translation="MTDERIQGYARGIFDMASGEGLLDRVEDELLTIARSFETSPELR
                     SALTDPQLPVDKKQGILEELIGGRASSL"
     gene            complement(220..768)
                     /gene="atpF"
                     /locus_tag="AC161X_09080"
     CDS             complement(220..768)
                     /gene="atpF"
                     /locus_tag="AC161X_09080"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HIR4989746.1"
                     /translation="MSHPTTTILAASEGGEGSAAELLLPAWPELVAGIIAFSIVFFFV
                     GKWVWPSLNKTIEARQAAIAGELTAAEVAKKEAESLLTDYRSQLAEAKTDANRVIEEA
                     RQQAEQLKTDVLAKAEADADQIRTKARDEASGEKARALADAKSQVGDISVDLAGKIVG
                     ESLDGNAHRVLIDRYLADLEKL"
     gene            complement(770..1027)
                     /gene="atpE"
                     /locus_tag="AC161X_09085"
     CDS             complement(770..1027)
                     /gene="atpE"
                     /locus_tag="AC161X_09085"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014010346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HIR4989747.1"
                     /translation="MDALVVLAQEGVGLTGNIGSGLAAIGYGLGAIGPGIGVGIVAGN
                     AVQAMARQPEVAGMLRTNMFIGMALSEALALIGFVVFFLAG"
     gene            complement(1064..1867)
                     /gene="atpB"
                     /locus_tag="AC161X_09090"
     CDS             complement(1064..1867)
                     /gene="atpB"
                     /locus_tag="AC161X_09090"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HIR4989748.1"
                     /translation="MILAFTCQPTEQPLCIPESILELFELPVLFGSVNRTMVYIFLVA
                     LVVIAALFVAYRKPRLVPTKFGVAVESIVDFVRNDIAIGVIGPGGERYAPYLLALFLY
                     ILVGNFFGITPFIMFPIGARMAIPAFLALLTYVVFVFAGFKKQGFTYVVHTIWPPGVP
                     VALKPLIGLIELVSIFIVRPFSLAVRLFANLVAGHTMIALLLGSGVFFIYAGATGMIS
                     PLKGFLVGPLWFVFGMAIYLLELLVLALQAYIFTLLSAVYIESSLHPAH"
     gene            complement(1864..2283)
                     /locus_tag="AC161X_09095"
     CDS             complement(1864..2283)
                     /locus_tag="AC161X_09095"
                     /inference="COORDINATES: protein motif:HMM:NF053722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cg1360 family ATP-synthase-associated protein"
                     /protein_id="HIR4989749.1"
                     /translation="MTPDQTKPPVDVEMKIARHTVRRAVYVGPLMIAIFWLFRGWDGA
                     WSSAIGVVVVVANFLLAGAVLSISARISLGAYHAAALIGFFLRLGLLTLTMLLIANYA
                     PIDRLAFGITAVVTYLVLIAAETVAVAKGKERELEWT"
     gene            complement(2280..2510)
                     /locus_tag="AC161X_09100"
     CDS             complement(2280..2510)
                     /locus_tag="AC161X_09100"
                     /inference="COORDINATES: protein motif:HMM:NF021073.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045259 - proton-transporting ATP
                     synthase complex [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HIR4989750.1"
                     /translation="MKQESTSKQVHSAVDQGWMQAGSFLNSVLAGTLLGYVLDLWLGT
                     DPWLVIIGIVLGSYSGFMKIWAMIRSQDEEQQ"
     gene            2609..>2896
                     /locus_tag="AC161X_09105"
     CDS             2609..>2896
                     /locus_tag="AC161X_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989751.1"
                     /translation="MSQPPTAVLLDVGGVFLLPSREHIRSALDQIGHRVPLDETLDRA
                     HYLAARVFPLEAGEAEFMGELWRRYLETYAEAVGVAPELRRDALEHLRNEYV"
BASE COUNT          560 a          881 c          945 g          510 t
ORIGIN      
        1 caaggaagag gctctgcctc cgatcagctc ttcgaggatg ccttgcttct tgtcgaccgg
       61 cagttgcggg tcggtgagcg ccgaccggag ctcgggtgag gtctcgaagg aacgggcgat
      121 cgtcagcagc tcgtcctcga ctcggtcgag gaggccttcg ccggacgcca tgtcgaagat
      181 acccctggcg tatccctgga tccgctcatc ggtcatgtgt tacagcttct ccaggtcggc
      241 gaggtagcgg tcgatcagga cgcgatgggc gttcccgtcg agggactcgc cgacgatctt
      301 cccggcgagg tcgacggaga tgtcgccgac ctgggacttg gcatcggcga gggcgcgcgc
      361 cttctccccg gaggcttcgt cccgtgcctt ggtccggatc tggtcggcgt cggcttccgc
      421 cttggcgagg acgtcggtct tgagctgttc ggcctgctgt ctcgcctcct cgatgacccg
      481 gttggcatcg gtcttggcct cggcgagctg cgacctgtag tcggtgagga ggctctctgc
      541 ctccttcttg gccacctcgg cggcggtcag ctcaccggcg atggccgcct gtcgagcctc
      601 gatggtcttg ttgaggctgg gccagaccca tttccccacg aagaagaaca cgatcgagaa
      661 ggcgatgatc ccggcgacca gctcgggcca cgccgggagc agcagctcgg cggcgctgcc
      721 ttcgccgcct tcgctggcgg ccaggatggt tgtcgtcggg tgggacactt cagccagcca
      781 ggaagaagac cacgaacccg atcagggcga gtgcctcgga cagggccatg ccgatgaaca
      841 tgttggtccg gagcattccg gccacttcgg gttgacgggc catcgcctgc accgcattgc
      901 cggcgacgat gccgacaccg atgccggggc cgatggcgcc cagaccgtat ccgatggcag
      961 cgagaccgct gccgatgttg ccggtgaggc ctacgccctc ttgtgccaag acgactagtg
     1021 cgtccattgg ttttgccttt ctctgcctat gggttgttcg gacctagtgg gccgggtgca
     1081 acgagctctc gatgtacacg gcggacagca acgtgaagat gtaggcctgg agtgcgagga
     1141 ccagcagctc caaaaggtag atcgccatac cgaagacgaa ccacagcggc ccgaccagga
     1201 atcccttgag cggcgagatc atcccggtcg cgccggcata gatgaagaag acgccggaac
     1261 cgaggagcag ggcgatcatg gtgtgacccg cgacgaggtt ggcgaacaac cggactgcca
     1321 gtgagaacgg tcggacgatg aatatcgaga ccaactcgat caggccgatc agcggcttca
     1381 gcgccaccgg gaccccgggt ggccagattg tgtggacgac gtacgtgaag ccctgcttct
     1441 tgaagccggc gaacacgaag accacgtagg tgagcaaggc gaggaaggcg gggatcgcca
     1501 tccgcgcacc gatcgggaac atgatgaagg gtgtgatgcc gaagaagttg cccacgagga
     1561 tgtagaggaa cagtgccagc aggtatgggg cgtacctctc cccgcccgga ccgatgacac
     1621 cgatggcgat gtcgttgcgg acgaagtcga cgatgctctc cacggccacc ccgaacttgg
     1681 tggggacgag ccgaggcttg cggtaggcca cgaacagcgc cgcgatgacc accagcgcca
     1741 ccaggaagat gtagaccatc gtccggttga ccgatccgaa cagcacgggc agctcgaaca
     1801 gctcgaggat cgactcgggt atgcacaacg gctgctccgt cggctgacag gtgaatgcca
     1861 ggatcacgtc cactcgagct ccctctcctt gcctttcgcc actgccaccg tctcggcggc
     1921 gatcaatacc agataagtca ccacggccgt gatgccgaac gccaaccggt cgatgggcgc
     1981 gtagttggcg atcaacaaca tggtgagtgt caacagcccc agccggagga agaaaccgat
     2041 cagggccgcg gcatgataag ccccgaggct gatcctggcg gaaatcgaga ggacggctcc
     2101 ggcgaggagg aagttggcga ccacgaccac gacgccgatt gcagagctcc aggctccgtc
     2161 ccaaccccgg aacagccaga agatggcgat catcaggggc cccacgtaga cggcgcgtct
     2221 gacggtgtgg cgggcgatct tcatctcgac gtcgaccggt ggcttcgtct ggtcgggcgt
     2281 cattgctgtt cctcgtcctg tgatctgatc atcgcccaga tcttcatgaa cccggagtac
     2341 gagccgagca cgatcccgat gatcaccagc caggggtcag tgccgagcca taggtcgagc
     2401 acatagccga gcagggtgcc ggcgagcacc gaattgagga atgagcccgc ctgcatccag
     2461 ccttggtcga ccgccgaatg gacctgcttc gaggtggatt cttgcttcac ggggtgagga
     2521 gcttgtgaag agattcacaa actctattcg gggaagtgga tcatggacaa atcgaaccta
     2581 gcaaaggaac cggataggtt cgaccctgat gagccaacca cctaccgccg tcctgctcga
     2641 tgtcggcggt gtcttcctgc tcccctcccg tgagcacatc cgcagcgctc tcgaccagat
     2701 cgggcatcga gttccactcg acgagacatt ggatcgtgct cactacctgg cggcacgcgt
     2761 cttcccgctc gaggctgggg aggccgagtt catgggcgag ctttggcgac gctatctgga
     2821 gacctacgcc gaagcggttg gagtggcccc cgaattgcgg cgagatgcct tggagcacct
     2881 tcgtaacgag tacgtg
//
LOCUS       DBNHXU010000370         5694 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1143451, whole genome shotgun sequence.
ACCESSION   DBNHXU010000370 DBNHXU010000000
VERSION     DBNHXU010000370.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5694)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5694)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5694
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1143451"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="AC161X_09110"
     CDS             <1..568
                     /locus_tag="AC161X_09110"
                     /inference="COORDINATES: protein motif:HMM:TIGR01926.1"
                     /note="This protein belongs to a clade of uncharacterized
                     proteins related to peroxidases such as the
                     alkylhydroperoxidase AhpD; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peroxidase-related enzyme"
                     /protein_id="HIR4989752.1"
                     /translation="AYVEVTPYDEATGVAKEEYDAAIGRAGRIWNIVSVQSQLPAVLR
                     ESMRLYKEVMFGPSPLSRAQRELLAAVTSSANSTRYCTIAHWHDYRDEVEDQLMVDQI
                     MEDWKSADMNEADRALVAFAEKLTWANSTVGPAEIATLRSHGFDDVAISSAVQVVAYF
                     NYINRVADGLGVDLEDWIATDGREAAVG"
     gene            561..2039
                     /locus_tag="AC161X_09115"
     CDS             561..2039
                     /locus_tag="AC161X_09115"
                     /EC_number="3.4.16.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013176911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004185 - serine-type carboxypeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S10 family peptidase"
                     /protein_id="HIR4989753.1"
                     /translation="MADEAKVEAATEKPLTPEVEEKSVAKQYRGTFGGREVAYTATAA
                     TIGVGTADDRRAAFFSVSYTEDGADPAVRPIMFAFNGGPGSSTVWLHLGLFGPKRVEL
                     DEEGFSVRIPGRLIENEYSILDVADVVLVDAIGTGFSTAEPKEKEKDYHHFARDIEAF
                     TEFIVSYLNRHGRWASPKYLAGESYGTTRGAAIAKELFSNQGVELNGIILISVALSFQ
                     TIASEEGTRVFHPGNELPFPLHLPTYAATAWYHGRLSESHQNRPLRELLDEVEEFALG
                     RYWTALAYGDQLDSEKRTEVLTKLSEYTGLSETYLDRYDLRIHINRFCKELLRDQRRT
                     VGRLDSRYTGIDRFADGDNLENDPSGDQMMGLFTSTLNHYLRTDLGYDNDSFYKSLSM
                     KVHEAWDYEDFKGHYVNTSDQLREVMVRSPKTLVFVANGYYDLATPHFATEYTFSHMG
                     LDESVRKNVRMEYYDAGHMMYVHKPSLERLAADLREFVNDTR"
     gene            complement(2059..3507)
                     /locus_tag="AC161X_09120"
     CDS             complement(2059..3507)
                     /locus_tag="AC161X_09120"
                     /inference="COORDINATES: protein motif:HMM:NF024949.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HIR4989754.1"
                     /translation="MSETQQLDPLVLGRRLRHHRKLRGMTLDQLGAAVGKPAPYLSLL
                     ENGKKQPRVNLVLDLATALEVDVADLLDPRPPNHRDALEISLLKSQESAFFEALNLPS
                     VRPTSRMDTETLTHLVGLHEALRQRSGLGTAGSEDVRRANGEVHQWLTEESGYLEGVE
                     RVAGEALETSGYSGEGPFSSRNLLDLAKHAGFEIHSIDDMPVFARSVIDLDLRRVYIA
                     QRDELRTRQARKAVLQTLAGFLLGHETDPDLDTFLRQRVETAYFAAAVLAPEGAVVPR
                     LIEAKDRHDLDVEDVKELFYVSYEMAAWRMANLASRHLSFPTHLIVSDQMGNVIKGYA
                     NDGVPVPRDDHGGIETQRLCKRWGTRMTFESHERFGTHHQYTDTPEGTFFCTTHVEAG
                     RDPSLAVTLGVRFADAHWFRGRETTTRETSTCPDPSCCRMPSEELSKKWSNKVIVSAR
                     SQNRILGLLAPDPYPELDMPEVLGLVEAHFDD"
     gene            3591..5147
                     /gene="aceB"
                     /locus_tag="AC161X_09125"
     CDS             3591..5147
                     /gene="aceB"
                     /locus_tag="AC161X_09125"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013178027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006097 - glyoxylate cycle [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HIR4989755.1"
                     /translation="MTETMKERLEQTLLTADAVAFLSDLERAFRERRAQILDARAGVQ
                     EGLDRGLRPHFLDSTRAIRESDWVIAPVPADLEDRRVEITGPVDARMMINALNSGARV
                     FMADFEDATTPTWLNLLEGQANLVDAYRRTLHHETAEKAYALADQVAVLFVRPRGWHL
                     VEKHFLVDGHAMSASLFDFGLYLFHGGRAAIAAGTGPYLYLPKLESHMEARLWNDVFV
                     WAQDRLDIPQGTIRVTVLIETILAAFEMDEILHELRDHITGLNAGRWDYIFSMIKKFR
                     DDPGFVLPDRAQVTMTVPFMRAYTELMVKTCHRRGAHAIGGMAAFIPNRWDPEVTETA
                     IARVTADKAREAAAGCDGTWVAHPDLVPVAMQEFDSVLGDRPNQVGRARDDVTVSAAD
                     LLDLDFPGEVTDEGVRANISVGIRYLASWLSGVGAAGIDNLMEDVATAEISRSQIWQW
                     IRHRVHTDGGTPVTGAMVKSIADEVEALLQSEGRFGDTAHLARQIFEEVAAGEEFVEF
                     LTLPAYEHID"
     gene            5157..>5694
                     /locus_tag="AC161X_09130"
     CDS             5157..>5694
                     /locus_tag="AC161X_09130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009073950.1"
                     /note="catalyzes the reversible formation of glyoxylate
                     and succinate from isocitrate; glyoxylate bypass pathway;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase"
                     /protein_id="HIR4989756.1"
                     /translation="MTTYTNEANRLADDWRNNPRWDGILRDYTAEEVVRLRGSIREQH
                     TLAIRMSQHLWELLTTEDYVSALGAISGGQATQMVKAGLKAIYLSGWQVAGDGNLAGE
                     VYPDQSLYPANSAPALVKRINNALRRADQIDWSEGDSEIDWFVPIVADGEAGFGGPLN
                     VFELTKSFIEAGAAGVHFE"
BASE COUNT         1080 a         1753 c         1807 g         1054 t
ORIGIN      
        1 ggcgtacgta gaagtaacgc cttacgacga agcgacgggt gtcgccaaag aggaatacga
       61 cgccgccatc ggccgcgccg gtcgcatttg gaacatcgtc tcggtgcaaa gccaactgcc
      121 ggcggtcttg cgggagtcga tgcgcctcta taaggaagtg atgttcgggc cctcccctct
      181 cagccgggcc cagcgcgaac tcctcgcagc ggttacctca agcgccaact ccacccgcta
      241 ctgcaccatc gctcactggc acgactaccg cgatgaggtc gaagaccagc tgatggtgga
      301 ccagatcatg gaggactgga agtcagccga catgaacgag gccgaccggg cgctggtcgc
      361 cttcgccgag aagctcacct gggccaattc cacggtgggg ccggccgaga tcgcgactct
      421 ccgctctcac gggttcgatg acgttgctat ctccagcgcc gtccaggtgg tcgcatattt
      481 caactacatc aaccgggtgg ccgacggact cggtgttgac ctcgaagatt ggatcgctac
      541 cgacggacgg gaagcagccg ttggctgacg aggccaaggt cgaggccgcc acagagaaac
      601 cgctgactcc tgaggtcgag gagaagtcgg tcgccaagca gtaccggggg accttcggcg
      661 gtcgcgaagt cgcctatacg gccaccgcag ccaccatcgg ggtcggcact gccgacgacc
      721 gacgggccgc cttcttctct gtgtcctaca ccgaggacgg tgcggatccc gcagtccgcc
      781 cgatcatgtt cgctttcaat ggcgggccgg gatcgtcgac ggtatggctc catctcgggc
      841 tcttcgggcc caagagggtg gagctggacg aagaaggctt ctcggttcgg atcccgggtc
      901 gactgatcga gaacgaatat tcgatcctcg acgtggccga tgtggtcctc gtcgatgcca
      961 tcgggaccgg gttcagcacg gcggagccca aggagaaaga gaaggactac caccacttcg
     1021 cccgggacat cgaggccttc accgagttca tcgtctccta tctcaatcgc catgggcgtt
     1081 gggcttcgcc caagtatctg gcgggagaga gttacgggac cacccggggc gcggccattg
     1141 ccaaggagtt gttcagcaac cagggcgtgg agctgaacgg gatcatcctc atctctgtgg
     1201 cgctgagctt tcagaccatt gcctccgagg agggcacccg ggtcttccat cccgggaacg
     1261 agctcccctt ccccctccac ctccccacct acgccgcgac tgcgtggtat cacggccggc
     1321 tctcggagag ccatcagaat cggccgctcc gcgagcttct ggacgaagtg gaggagtttg
     1381 ccctggggcg gtattggacg gccctcgcct acggggacca actcgactcg gagaagcgga
     1441 ccgaggttct gaccaagctc agcgagtaca ccgggctgtc cgagacctat ctcgaccgct
     1501 acgacctccg gatccatatc aatcgcttct gcaaggagct gttgcgcgat cagcgcagaa
     1561 ccgttgggcg tctcgactcc cgctacacgg ggatcgaccg cttcgccgac ggggacaacc
     1621 tcgagaacga tccgagtggt gatcagatga tgggtttgtt cacctccacc ctcaatcact
     1681 acctccgaac agacctcgga tacgacaacg actccttcta caaatcgttg tcgatgaagg
     1741 tccatgaggc gtgggactac gaggacttca agggccacta cgtcaacact tcggaccagc
     1801 tgcgagaggt gatggtccgc tctccgaaga ctctggtctt cgtcgccaac ggctactacg
     1861 acctggccac cccccacttc gcaaccgagt acacgttcag ccacatgggg ctcgatgagt
     1921 cggtgcgcaa gaacgtccgg atggagtact acgacgcggg gcacatgatg tacgtccaca
     1981 agccctcact ggagcgactg gccgcggatc tccgcgagtt cgtcaacgac acccgttagg
     2041 ggtcagtcgg tcgcggaatc aatcgtcaaa gtgagcctcg acgaggccca gaacctcggg
     2101 catgtccaat tccggataag gatccggggc gaggagacca aggatccggt tctgggagcg
     2161 agcggagacg atcaccttgt tcgaccattt cttgctgagt tcctccgacg gcatccggca
     2221 acaagaaggg tcggggcagg tcgaagtctc ccgggttgtc gtctcccgcc cacggaacca
     2281 gtgggcgtca gcgaatctga cgccaagggt cactgccaga gagggatccc gacccgcttc
     2341 gacgtgggtg gtgcagaaga aggtcccctc cggggtgtcg gtgtactggt gatgggtgcc
     2401 gaaacgctcg tgggactcga aagtcatccg cgtcccccac cgcttgcaga gacgctgggt
     2461 ctcgatcccg ccatggtcgt cgcggggcac gggaaccccg tcgttggcgt agcccttgat
     2521 cacgttcccc atctgatcac tgacgatgag atgagttggg aagctcaggt ggcggctggc
     2581 gagattggcc attctccagg cggccatctc gtaggaaacg tagaacagct ccttgacgtc
     2641 ctcgacatcg aggtcgtgtc ggtccttggc ctcgatcagc cgggggacca cggctccttc
     2701 gggagccagc actgcagcgg cgaagtatgc cgtctcgact cgctgccgga ggaaggtgtc
     2761 caggtcgggg tcggtctcgt gaccgaggag gaacccggcc agggtctgga gcactgcctt
     2821 gcgagcttgc cgggtgcgca gctcgtctcg ttgcgcgatg tagacacgtc gtaggtcgag
     2881 gtcgatgacc gatcgagcaa acaccggcat gtcgtcgatc gagtgaatct cgaatccggc
     2941 gtgcttggcc agatccagga gattgcgcga ggagaacggg ccctcgcccg agtacccact
     3001 ggtttccagg gcttcaccgg cgactcgttc gactccttct agatatccgc tctcttcggt
     3061 cagccattga tgaacctcgc cattggcgcg gcggacatct tcagatccgg cagtgcccaa
     3121 tccggagcgc tgacgaagcg cttcgtggag gcccaccaaa tgagtcaatg tctcggtgtc
     3181 catcctcgag gtgggtcgca ccgatgggag gttgagggct tcgaaaaagg ccgactcctg
     3241 ggatttgagg agcgagatct cgagggcatc acggtggttg ggaggtctcg ggtcaagaag
     3301 atcggcgacg tcgacctcga gcgcggtcgc cagatcgagc accagattga cccgaggctg
     3361 cttcttgccg ttttcgagca gcgacagata gggcgccggc ttgccgaccg ccgctcccaa
     3421 ctggtcgagg gtcatcccac gcagcttgcg gtgatggcga agacgtcttc ccaggaccag
     3481 agggtcgagt tgttgggttt cgctcatgtt gtctatctcc gaaagatgga cagttcttat
     3541 actgtgactt gcgtccaatt ctagtcctca acgaaagaca ctccactctt atgacagaaa
     3601 cgatgaagga acggttggag cagactttgc tgactgccga cgccgtcgcc ttcctcagcg
     3661 acttagaacg ggcgttccgg gagcggcgtg ctcagatcct cgatgcccgt gccggcgtcc
     3721 aggaggggct cgaccgtggg ctccgtcccc acttcctcga ttcgacgaga gccatcaggg
     3781 agtcggactg ggtgattgcc ccggtccccg ccgacctcga ggatcgccgg gtcgagatca
     3841 ccggtccggt cgacgcccgg atgatgatca atgccctcaa ctccggggcc cgggtgttca
     3901 tggccgattt cgaagacgcc accacgccga cctggctcaa tcttctcgag ggccaggcca
     3961 atctcgtcga cgcctatcgc cgaacactgc accatgagac cgcggaaaag gcctatgccc
     4021 tcgcggacca ggtcgcggtt ctgtttgtgc gtccccgggg atggcatctc gtcgagaagc
     4081 acttcctcgt cgacgggcac gccatgtcgg cttcattgtt cgacttcggc ctctacctct
     4141 ttcatggggg ccgggctgcc atcgcagccg gaaccggccc ctatctgtat ctgcccaagc
     4201 tggagtccca catggaggct cgcctctgga acgacgtttt cgtctgggcc caggaccgtc
     4261 tcgacatccc ccagggcacc atccgtgtca cggtcctcat cgaaaccatc ctcgctgcct
     4321 tcgagatgga cgagatcctc cacgagcttc gtgaccacat caccgggctc aacgcgggcc
     4381 gttgggacta catcttctcg atgatcaaga agttccgcga cgacccgggg ttcgtccttc
     4441 ccgaccgggc tcaggtgacg atgacggtgc ctttcatgcg ggcctacacc gagttgatgg
     4501 tcaagacctg tcatcgccga ggggcccatg ccatcggggg catggccgct ttcatcccta
     4561 atcgctggga tcccgaggtc accgagacag ctatcgccag agtcacggcc gacaaggcgc
     4621 gcgaggcggc cgccggctgt gacggcacct gggtcgccca tcctgacctg gtccctgtgg
     4681 cgatgcagga gttcgactcg gtgctcgggg atcgcccgaa tcaggtgggc cgggcccgcg
     4741 acgacgtcac cgtctccgca gcagatctgc tcgacctgga ctttcccggc gaggtcaccg
     4801 atgagggtgt cagggcaaac atctcggtcg gcatccgcta cttggcgtcg tggctctccg
     4861 gggtgggggc ggccggtatc gacaatctga tggaagacgt cgccactgcc gagatctcac
     4921 gatcccagat ctggcagtgg atcaggcatc gggttcacac cgacgggggg actccggtaa
     4981 ccggggccat ggtgaagtcg atcgccgatg aggtggaagc actcctccag agcgagggcc
     5041 gattcggaga caccgcgcac ctggcccgtc agatattcga agaggtagcg gcaggggagg
     5101 agttcgtcga gttcctgacc ctccccgcat atgagcacat cgattaggga gaagacatga
     5161 ccacttacac caatgaggcc aaccgtctgg ccgacgattg gaggaacaat ccacgctggg
     5221 acgggatcct ccgggactac accgccgaag aggtggtgag gctgcggggg agtattcgcg
     5281 aacagcacac gctggcgatt cggatgtcgc agcacctgtg ggagttgctc acgacggagg
     5341 attatgtgag tgccctgggg gcgatcagcg gtgggcaggc gacccagatg gtgaaggccg
     5401 ggctgaaggc gatctatctg tccggctggc aggtggccgg cgatggcaac ctggccggcg
     5461 aggtctaccc cgatcagtcg ctgtatccag ccaactccgc cccggcgctg gtgaagcgga
     5521 tcaacaacgc cttgcgccga gccgaccaga tcgactggtc ggagggcgac agcgagatcg
     5581 actggttcgt gccgatcgtg gccgacgggg aggccggatt cggcggaccg ttgaacgtct
     5641 tcgagttgac caagagcttc atcgaggcgg gagcggccgg cgtccatttc gagg
//
LOCUS       DBNHXU010000371         3186 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_944289, whole genome shotgun sequence.
ACCESSION   DBNHXU010000371 DBNHXU010000000
VERSION     DBNHXU010000371.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3186)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3186)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3186
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_944289"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="AC161X_09135"
     CDS             complement(<1..395)
                     /locus_tag="AC161X_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989757.1"
                     /translation="MTDSALALLAAVAATVFAVDLWLDYRRRPRPHIAAYGIGMTMFA
                     VATWAFFIGLSFGWTGPVYRTFYLFGAILNIPFLALGSMFLVVGRRSGHVMAIALGAF
                     TAIATTLTATVPFANPLPESGVPHEIFAEG"
     gene            complement(392..1411)
                     /locus_tag="AC161X_09140"
     CDS             complement(392..1411)
                     /locus_tag="AC161X_09140"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011564157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989758.1"
                     /translation="MTSATDRLAIRARDLTKSYGDAVALDHFSLDVWEGGVVTLLGPS
                     GCGKTTALRVIAGFESGSGSIEIHGRTVLDADTFVPPEKRKVGMVFQDYALFPHMTVG
                     ANVGYGLPRGDRSARVGQVLELVGLTGLENRMPNELSGGQQQRVALARALAPEPEVIL
                     LDEPFSNLDASLRDRVRRELRSILTEARTTAVFVTHDQEEALATSDIVAVMKDGRILQ
                     ADTPAELYSNPSDPWVAEFLGDADVIEAVARNGRVDTPVGGFDTALDGDVSVIVRPEN
                     IQISLGETPNAVVAGSEFFGHDQLVTLALTGGTRVRARLGPRPVYRIGEACRARAIDA
                     RVFAR"
     gene            complement(1395..2969)
                     /locus_tag="AC161X_09145"
     CDS             complement(1395..2969)
                     /locus_tag="AC161X_09145"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989759.1"
                     /translation="MTAPLVLPRIDPGPVRHRSSRGWWLAAGVALLPGLIPPISLAIL
                     VLGRGMTLAIPIDRLLELFASTLALTVAVTATALVIGLATAWVTTRVALPGRRSWMIL
                     AALPLVVPSYVAALSLIAATGPNGVITEVPTPYGFVGAWLVLSIFTAPLVHLTVIPGL
                     RAIDTATEEAATGLGAGKWKVFRTVTLPQLRPAMVSSALLVGLYVVSDFGAVSLLRYD
                     TFTRAIYALYRGQIDRRPAATLSLILMILAVLILFVERRTRGRAALYSRRPRRNRPLL
                     RLSTWPMALALFLIAAYALIALGMPLYVMAVTVGRGLEAGQVIGTTLGAETVRSLGVA
                     ILAALVTAFAAFPVAMVTTRRPGRLSGWVESVVWGTYALPHITVGVAAIAFALQWARP
                     LYQTLIFLLVIYVALFLPQAAGSTQDSLRRASPDLEDASRGLGRSPFSTLLHITLPLA
                     RPGLMAGGALVFLSAMKELPATILLRPNGFETLAIRVWSSTSEGFLTRASAAGLVLLV
                     VSIVPLFLVMSRDLGD"
     gene            complement(2971..>3186)
                     /locus_tag="AC161X_09150"
     CDS             complement(2971..>3186)
                     /locus_tag="AC161X_09150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter substrate-binding protein"
                     /protein_id="HIR4989760.1"
                     /translation="EPAQRFVDFLLSEESQAFFATDTFEYPLIDGVPQPDGAPPLSEI
                     NSPDIDLSELASVLDLATRLVAEAGLV"
BASE COUNT          640 a          965 c         1107 g          474 t
ORIGIN      
        1 ccctcggcaa agatctcgtg cggaacgccc gactcaggca ggggattggc gaaagggaca
       61 gtggctgtga gcgtggtggc gatcgcggtg aacgcaccaa gagcgatcgc cattacatgt
      121 ccggatcgcc ttcccaccac gaggaacatc gagcccagag caaggaaggg aatattgaga
      181 atggctccga acagatagaa ggtgcggtag acaggcccgg tccacccaaa gctgagaccg
      241 atgaagaacg cccaggtggc tacggcgaac atggtcattc cgattccgta ggcggcgatg
      301 tggggtcgcg gccgcctgcg atagtcgagc cacagatcga cggcgaacac ggtggcggcg
      361 acggcagcca gaagggccag agctgagtca gtcatcgggc gaagactctg gcgtcgatgg
      421 cccgggcccg gcacgcctca ccgatccggt acaccggtcg ggggccgaga cgggcgcgca
      481 ccctggtgcc tccggtcagg gccaaggtca ccagctggtc atgaccgaag aactcgctcc
      541 ctgccaccac ggcgttgggg gtctcgccta gcgagatttg gatgttctcg gggcggacga
      601 tcaccgacac atcgccatcg agtgcggtgt cgaaccctcc gaccggggtg tcgacccgac
      661 cgttgcgcgc aaccgcctcg atgacgtcgg cgtcgccgag gaactcagcg acccaggggt
      721 cgctcgggtt ggagtagagc tcagccgggg tgtcggcctg gaggatcctg ccgtctttca
      781 tcactgccac gatgtcagag gtggccagcg cctcttcctg gtcatgggtg acgaacaccg
      841 ccgtggtccg agcctcggtg agtatcgagc gcaactcgcg tcgcacccgg tcgcgcaagc
      901 tggcgtccag attggagaac ggctcgtcga ggaggatgac ttcgggctcc ggtgccaagg
      961 cacgggcgag ggcgaccctt tgctgttgcc cgccggaaag ctcgttgggc attcggtttt
     1021 cgagcccggt caacccgaca agttcgagga cctggcccac gcgcgccgag cgatcgcctc
     1081 tgggcaggcc gtaacccaca ttcgccccca ccgtcatgtg cggaaacaag gcgtagtcct
     1141 ggaacaccat gcccactttt cgcttctcag gtgggacgaa ggtgtcagcg tcgaggacag
     1201 tccggccgtg gatctcgatc gagccgctcc cactttcgaa acccgcgatc accctgagcg
     1261 ccgtcgtctt tccgcatccc gacgggccga gaagggtgac cacccctccc tcccacacgt
     1321 cgaggctgaa atgatcgagg gccaccgcgt cgccatagga cttcgtcagg tccctggccc
     1381 ggatggcgag acgatcagtc gccgaggtca cgtgacatca caaggaagag tggcacgatc
     1441 gaaaccacaa ggagaaccag gccggcggcg gacgccctgg tcagaaagcc ttcggatgtg
     1501 gagctccaga cccggatggc cagagtctcg aagccgttgg gccgaaggag gatggtcgcc
     1561 ggcagctcct tcatcgcgct cagaaacacc agtgcccctc ccgccatcaa tccgggtctg
     1621 gccagcggca acgtgatgtg aagcaaagtg ctgaaggggc tgcgaccgag cccgcgcgac
     1681 gcatcctcga ggtccgggct cgcccttctc agcgagtcct gggtcgatcc cgcagcttgg
     1741 ggaaggaaga gtgcgacata gataacgagg agaaagatca acgtttggta gaggggtcgg
     1801 gcccattgca gggcaaacgc tatggcggcg acgccaaccg tgatgtgagg cagggcgtag
     1861 gtgccccaga ccaccgattc cacccatccc gagagtcggc ctggccgccg ggtggtgacc
     1921 atggccacag ggaaggcagc gaaggcggtg accagcgcag caaggatcgc cacgccaaga
     1981 gatcgcacgg tctccgcccc cagggtggtg ccgatcacct gaccggcctc gagcccgcgc
     2041 ccaactgtga cggccatgac atagagaggc attcccaggg caatcagcgc atatgcagcg
     2101 atgagaaaca gagcaagggc catcggccag gtcgacaacc tgagtaaggg ccggttgcgt
     2161 cgtggccttc tcgaatagag ggcggcgcgg cctcgggtgc ggcgctccac gaacaggatc
     2221 aggacggcca aaatcatcag gatcagggag agggtggccg ccggtctccg gtcgatctgg
     2281 ccccggtaga gggcgtagat cgctctggta aaagtgtcgt agcggagcag ggagactgcc
     2341 ccgaagtctg atacgacgta gaggccgacc aacaacgccg acgagaccat ggcggggcga
     2401 agctggggca gcgtgacggt cctgaagacc ttccatttcc cggcgccaag gccggtggcc
     2461 gcttcctccg tggcggtgtc gatggcgcgc aatcccggga tgacggtcag gtggaccagt
     2521 ggtgccgtga agatcgagag gaccagccag gcgcctacga agccgtaggg agtcggcacc
     2581 tccgtgatga cgccgttggg gccggtggca gctatcagac tcagcgccgc gacgtaggac
     2641 gggactacga ggggaagggc ggccaggatc atccagctcc ttcggccggg gagggcgacg
     2701 cgggtggtga cccatgcggt ggcaaggccg atcaccaggg cggtggcggt gacggcgacc
     2761 gtcaacgcca aagtgctggc gaagagctcg aggagccgat caatagggat cgcgagcgtc
     2821 atgcctctcc cgagcaccag gatggcgagg ctgatcggtg ggatcagccc gggcaataga
     2881 gcaacgccgg cagccagcca ccacccgcga cttgaccggt gccggacggg cccggggtcg
     2941 atcctgggaa ggaccagggg ggcggtcaac tcagaccagc ccggcctcgg ccaccaggcg
     3001 agtggcgagg tcgaggacgc tggctagttc cgataggtcg atatcgggcg agttgatttc
     3061 tgagagtgga ggcgccccat cgggctgggg gacgccatcg atcagtggat actcgaaggt
     3121 gtcggttgcg aagaatgcct gcgactcttc cgagagcaag aagtcgacga aacgctgggc
     3181 gggctc
//
LOCUS       DBNHXU010000372         2008 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_944475, whole genome shotgun sequence.
ACCESSION   DBNHXU010000372 DBNHXU010000000
VERSION     DBNHXU010000372.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2008)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2008
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_944475"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..729)
                     /gene="pth"
                     /locus_tag="AC161X_09155"
     CDS             complement(97..729)
                     /gene="pth"
                     /locus_tag="AC161X_09155"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: protein motif:HMM:NF013368.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - peptidyl-tRNA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HIR4989761.1"
                     /translation="MSRLLRTRKKAKAANSQVHMVIGLRNPGSDYEGTRHNAGYEAVA
                     RLVGRHDAKFTRAPSRLRCEVAQIGAGESRFLTVVPSTFMNDSGSAVRAAVDYYGVDL
                     SRTLVLHDDIDLAFGRLRLQVDGGNGGHNGIRSLEKALGTTEFSRLKIGVGRPPGSMD
                     PADFVLRPFAKAERAEIEVVLEEAADVAELWLSDPARAQEMAALRGRDMG"
     gene            complement(683..1345)
                     /locus_tag="AC161X_09160"
     CDS             complement(683..1345)
                     /locus_tag="AC161X_09160"
                     /inference="COORDINATES: protein motif:HMM:TIGR00731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25"
                     /protein_id="HIR4989762.1"
                     /translation="MPEVSLRAQTGRSTGSRDSRRVRKTGDVPAIVYGKGTEPVSIAV
                     NAHDLHVVLHTEAGANALINLEIEGGATLLTMARVIERHPFRNEYRHIDFVTVSLDEI
                     VTAEVAIHFEGSPIGVTNGGVFSPQRTHVVIEALPNAIPSAIMLDVSEVEIGDSLRIG
                     DLPVIEGVTYTEDPDAVVMSVTTMAAEEVVEPEGELEGEGEEPAEGEQAAEDAQEGES
                     GE"
     gene            complement(1380..>2008)
                     /locus_tag="AC161X_09165"
     CDS             complement(1380..>2008)
                     /locus_tag="AC161X_09165"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF042734.5"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase family protein"
                     /protein_id="HIR4989763.1"
                     /translation="EVSGRLAGDGATGLLLAHGAGTNQDHRVIVGLRDRLAAVGLRVM
                     TFNYPYTERGSKSPDRADRLVECHRAAAEVLVAQVDLLFLAGRSMGGRISTLLVAEGY
                     PAAGVILYAYPLHPPGKPESLRVSHFPQVTVPLLFFQGTRDSLSRMDLFETHIGSLPN
                     ATIDILDGATHSLTGGGWSEGAMFEHLTSQTVAWIDALPSGTRDGTRP"
BASE COUNT          360 a          644 c          635 g          369 t
ORIGIN      
        1 ctcccgaccc gtcgaaaacc atgaaccggg tcgaagtcgt cccttgatcc agagcaccga
       61 cgaaaccagg catggtgcga cgttatcggg cggggattag cccatgtcgc gaccgcggag
      121 ggccgccatc tcctgggccc gggccgggtc gctgagccag agctcggcca catcggccgc
      181 ttcctcgagg accacctcga tctcggctct ttctgccttt gcaaagggac ggagaacgaa
      241 gtctgccgga tccatcgacc ctgggggtcg accaacaccg atcttgagcc ggctgaactc
      301 agttgttccc agcgccttct ccagagagcg gatcccgttg tggccgccat ttcccccgtc
      361 tacctgaagt cgaagacggc cgaaggcgag gtcgatatca tcatggagca ccagcgttcg
      421 cgagaggtcg actccgtagt agtccaccgc ggctcgcacc gccgagccgg agtcattcat
      481 gaatgtcgac ggcaccacag tcagaaagcg actttcgccg gcgccgatct gggctacctc
      541 gcagcggagc cgtgatgggg ccctggtgaa tttggcgtca tgacggccga ccagcctggc
      601 caccgcttcg tatccggcgt tgtgcctggt gccctcgtaa tcgctccccg ggttgcggag
      661 accgatcacc atgtgaacct ggctattcgc cgctttcgcc ttcttgcgcg tcctcagcag
      721 cctgctcacc ctctgctggc tcctctccct cgccctcgag ctcaccctcg ggctccacga
      781 cctcttcggc agccattgtc gtgacactca tgaccacggc atcggggtcc tcggtgtagg
      841 tgacgccctc gatcacggga aggtcgccga ttcggagcga atcaccgatc tcaacctcgg
      901 aaacgtcgag catgattgcg gagggaatgg cattcggcag cgcctcgatg acgacatggg
      961 tgcgttgcgg cgagaacacg ccaccattgg tgactccaat cggcgagcct tcgaagtgga
     1021 tcgccacttc ggcggtgacg atctcgtcga gggacaccgt cacgaagtcg atgtggcggt
     1081 actcgttgcg gaatgggtgg cgctcgatca cccgggccat ggtgagaagg gtggcgccgc
     1141 cttcgatctc gaggttgatc agggcattgg ctccggcctc ggtatgaagg acaacgtgaa
     1201 gatcgtgggc gttgaccgcg atcgagaccg gctcggtgcc tttgccgtag acgatggcag
     1261 gcacgtcgcc cgtcttgcgg acgcgacgcg agtcacgcga tccggtgctc cgcccggttt
     1321 gggcgcgcag actgacttcg ggcatcgaaa gactccttgg aggaagagat gggcgccaat
     1381 tatggcctgg tcccgtctct tgtgccagac gggagggcgt cgatccaggc caccgtttgg
     1441 gaggtgagat gctcgaacat cgctccttca gaccaaccgc cgcccgtcag cgagtgagtg
     1501 gctccgtcca ggatgtcgat cgtcgcattg ggaagcgagc cgatatgcgt ttcaaagaga
     1561 tccatccggc tcagcgagtc tcgggtccct tggaagaaga gaagggggac ggtcacctgc
     1621 ggaaagtggg acacccgcaa ggattcgggc ttccccggcg ggtgtagcgg gtaggcgtac
     1681 aggatcacgc ccgccgccgg atacccctcg gctaccagca aggtggaaat tcgccccccc
     1741 atcgaccttc cagccaggaa caacaagtca acctgggcca ccaacacctc cgcggctgcc
     1801 cggtggcatt cgacgaggcg atccgctcga tccgggctct tcgagcctct ttcggtgtat
     1861 gggtagttga aggtcattac gcgaagcccc acagcggcca gccggtcgcg gagcccgact
     1921 atcactcggt gatcctggtt ggtgcccgcg ccatgggcga ggagcagccc ggtggcaccg
     1981 tcaccggcta ggcgcccgga cacctcgc
//
LOCUS       DBNHXU010000373         2038 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_548194, whole genome shotgun sequence.
ACCESSION   DBNHXU010000373 DBNHXU010000000
VERSION     DBNHXU010000373.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2038)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2038
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_548194"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="AC161X_09170"
     CDS             <1..864
                     /locus_tag="AC161X_09170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010987513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989764.1"
                     /translation="ALVAEGHVLVEDVPGVGKTQLAKSVARSIDSIFNRIQFTPDLLP
                     SDVTGVSVWDRNVSEFEFKPGPIFANLVVGDEINRASPKTQSALLEAMEERQVTSDGT
                     TRELPLPFMVMATQNPLEHEGTYPLPEAQLDRFMMRVVIGYPSRVKELEILDTHAVRS
                     TFFDLEPVVSVEEVAEMITIAQSVAVSPAVKTYIIDLVEGTRNHSDVLLGASPRAALF
                     LQRLGRAMAASQDREYVTPDDVKALAHPVLEHRMSLRPEAQMRGETLTGMIDEIMTRL
                     RVPGTTSRIAT"
     gene            864..>2038
                     /locus_tag="AC161X_09175"
     CDS             864..>2038
                     /locus_tag="AC161X_09175"
                     /inference="COORDINATES: protein motif:HMM:NF013998.8"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HIR4989765.1"
                     /translation="MPTLRGWAFSGAGLALIVLWYALGDIELLLIALFLVIAQIFALA
                     LVRARRSQLEVNRRLGSATVHDGDTTSVTLLVENQSKRAIGNISIEDDVNQLGVASFE
                     VARLRKGESTTATYRVTCRPRGVYRVGPTMVSISDPLGLAETRGPVGPVDRIVVYPAL
                     EELTGFPIVRGQDPAMQASRPEHSRRGGEDFYTLREYQRGDDLRRVHWPSSAKTDELM
                     IRQLETPWQSRALVLLDVRDGSYESREAFEKAVSGAATIVTHLVGSGFDADLWAGDAD
                     PIDASRYGSAMERLALVQPDSAIDIQAVATRIRQKGGGGALVIITGVADRSLLSVQRL
                     LARDYPTTVLMTVSSSTSQTLVGFHRFGVATVSIEPDQLWAPAWVTTMRTAWTTASVG
                     "
BASE COUNT          386 a          663 c          621 g          368 t
ORIGIN      
        1 gcgctcgtcg ccgaaggcca cgtcctggtc gaagacgttc ccggagtggg caagacccaa
       61 ctcgccaagt cggtggcccg gtcgatcgac agcatcttca accggatcca attcaccccc
      121 gacctcctcc cctccgacgt gacaggagtg tcggtatggg atcgcaatgt cagcgagttc
      181 gagttcaagc cggggccgat cttcgccaac ctggtggtgg gcgacgagat caaccgggca
      241 agcccgaaga cccaatctgc ccttctcgaa gcgatggaag agaggcaggt gacgtccgac
      301 ggcacgaccc gggaattgcc cctccccttc atggtcatgg cgactcagaa ccctttagag
      361 cacgaaggca cgtatcccct tcccgaggct caattggacc gtttcatgat gagagttgtg
      421 atcggatatc cgagccgggt gaaggaattg gagatcctgg acacgcatgc ggttcgatcc
      481 acctttttcg acctcgaacc ggtggtctcg gtggaagaag ttgccgagat gatcacgatc
      541 gcgcagagcg tggcggtctc accggccgtc aagacctaca tcatcgacct ggtcgaaggg
      601 acgaggaacc attccgatgt cttgctcgga gcctcacccc gcgctgcact cttcctgcaa
      661 cgcctgggcc gggccatggc cgcatcccag gatcgtgagt acgtgacccc ggacgacgtg
      721 aaggccctgg cgcatcctgt gctcgagcat cgaatgtccc tgcggccaga ggctcagatg
      781 cggggcgaga ccctgaccgg catgatcgac gagatcatga ccaggctcag ggtgccggga
      841 accacgtccc ggatcgccac ctgatgccaa cccttcgggg ctgggctttc tcaggggccg
      901 gtctcgcttt gattgtcctc tggtacgccc tcggcgacat cgaactgctc ctcatcgcat
      961 tgttcctcgt gatagcgcag atcttcgccc tcgccctggt gcgtgcccgg cgctcccagc
     1021 tcgaagtcaa cagacgactc ggctccgcca ccgtccatga tggcgacacc accagtgtca
     1081 ccctgctcgt cgagaatcaa tcgaaacggg cgatagggaa catctcgatc gaagacgatg
     1141 tcaaccagct cggagtggcc agcttcgaag tcgcccggct tcggaagggc gaatcgacaa
     1201 ctgctaccta tcgcgtcacc tgccggcccc gcggggtgta ccgggttggc ccgaccatgg
     1261 tcagcatctc agatcccctc gggctcgcag aaacccgcgg gccagtcggc ccggtcgacc
     1321 ggatcgtggt ctatcccgca ctcgaggagc tcaccggatt tcccatcgtg aggggccagg
     1381 atcccgcgat gcaggcttcc cggcccgagc acagccggcg aggcggtgag gacttctaca
     1441 ccctccgcga atatcagcgt ggagacgacc tgagaagggt tcattggcca tcgtcggcga
     1501 agaccgacga attgatgatc cgccaactgg agaccccatg gcagtcgcga gcactggtcc
     1561 tgctcgacgt gcgggacggc agctatgagt cgagggaggc ctttgagaag gcggtgtcgg
     1621 gagccgccac catcgtcacc catctggtcg gctccggctt cgacgcagac ctgtgggccg
     1681 gcgacgccga ccccatcgac gcttcacgct acggctcggc catggagcgc ctcgcactgg
     1741 tccaacccga ttcggcgatc gacatccagg cagtcgccac caggattcga cagaagggcg
     1801 gcggcggcgc tctggtgatc atcaccgggg tcgcggacag gtccctgctt tcggtgcaac
     1861 gactgcttgc ccgtgactac ccgaccacgg tcttgatgac ggtctcatcg tcaacttcgc
     1921 aaactctggt cggatttcac cgattcgggg tggccacggt gtcaatcgag cccgaccaac
     1981 tctgggctcc ggcttgggtc acgactatga ggacggcatg gacaacagcc tcggtcgg
//
LOCUS       DBNHXU010000374         2353 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2135014, whole genome shotgun sequence.
ACCESSION   DBNHXU010000374 DBNHXU010000000
VERSION     DBNHXU010000374.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2353)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2353)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2353
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2135014"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..618
                     /locus_tag="AC161X_09180"
     CDS             <1..618
                     /locus_tag="AC161X_09180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989766.1"
                     /translation="APIGTKGFWSALESDLKGYLVANQRATVFRNASLRIYSRVGETQ
                     DEFVARAAEAAEGAGDEAMAKLKDTYAARIDRVRDSISKAENRVADLQADATSRQGDE
                     LISGAGDLLGAFLGGRKRSNPLGQVARRRAASQKAQTRADNAGEALADEQQELAELES
                     ELAAKISEISDQSGAKASQIETVDIPLEKTDIRVVELKLVWVPIR"
     gene            complement(690..1676)
                     /locus_tag="AC161X_09185"
     CDS             complement(690..1676)
                     /locus_tag="AC161X_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018654987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HIR4989767.1"
                     /translation="MAAAETRIEKTEKPRPRLHSKLTDERGRLTTDLVSQYLTAIGEY
                     DLLTAEQEVELAQKIEAGEEAAERLEKGEYSGKREEMELRRLSRRGAQAKDDFLTANL
                     RLVVANARRYANTSGIDFLDLIQEGNLGLIRAVEKFDWRKGFKFSTYATWWIRQAITR
                     AIADKSRTVRIPVHLHDTLAAVRAAQASLKAELGRDPRPEEIAEEAGVTADKVELALS
                     VADTVSLEQPIGEDGAQLGDFIEDEDAADPVQMTEEMDVANSLRISIERLPEREGRIL
                     ALRYGFYDGVPRTLEEIGDEFNLTRERIRQLEKLALCRLRHPSFGIREADLV"
     gene            complement(1885..>2353)
                     /locus_tag="AC161X_09190"
     CDS             complement(1885..>2353)
                     /locus_tag="AC161X_09190"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SixA phosphatase family protein"
                     /protein_id="HIR4989768.1"
                     /translation="DASYGSDHDRPLAPRGHRSATVMGRVLTAWDEVPDLVITSSAAR
                     ARATAEEVVRAGSWKCPITVESDLYGTGPGTVLGVAEGIATDEERIMLVGHEPTWSML
                     AEQITGGRVEMRTAGVAAIDLLADTWEGLVEGRGWLRFVINPRMFFGSEWDRE"
BASE COUNT          448 a          744 c          732 g          429 t
ORIGIN      
        1 gcgccaatcg ggacaaaggg cttctggagc gcgctggaat ccgatctcaa gggctacctg
       61 gtggccaatc agagggccac ggtcttcagg aacgcatccc tgcgcatcta ttccagggtc
      121 ggcgagaccc aggacgagtt cgttgcgcgg gccgcagagg ccgccgaggg agcgggcgac
      181 gaggcgatgg caaagctcaa ggacacctac gcagcgagga tcgatcgggt tcgcgactcc
      241 atctccaagg cggagaatcg ggtagctgac ctccaggcgg acgccacctc acgtcaaggc
      301 gatgaactga tctccggagc cggggatcta ctcggtgcat tcctgggtgg gcgcaaaagg
      361 tccaacccgc tcggtcaggt ggcccgccgt cgcgccgcct cacagaaagc acagacacgg
      421 gccgacaacg caggcgaagc gcttgccgat gagcaacagg aattggccga gctcgaaagt
      481 gagttggccg ccaagatcag cgagatcagt gatcagtccg gggcgaaggc ttcgcagatc
      541 gaaacggtgg atatcccgct cgagaagacc gacatccggg tggtggagct gaaattggtc
      601 tgggtgccga tccgctgacc atcttcgttc tcgtaattgg gacctacagg gggtcatatc
      661 cggaccccac ggtctcaatt acgggtgggt cagaccaggt cggcttcccg gatcccgaac
      721 gagggatgac ggagacggca cagtgccagc ttctccaact gacgaatacg ctcccgggtt
      781 aggttgaact cgtcgccgat ctcttccaaa gtccggggta ccccgtcgta gaagccatag
      841 cggagggcca ggatccgccc ctcacgctcg ggtaggcgct cgatggaaat ccgcaatgag
      901 ttggcgacat ccatctcctc ggtcatctgc accgggtcgg cggcgtcctc gtcctcgatg
      961 aagtcgccga gttgggcgcc atcctcaccg atcggctgct ccagggaaac agtgtcggcc
     1021 acgctcaggg cgagctcgac cttgtccgct gtcacgcccg cttcctcagc aatctcttcc
     1081 ggccgtgggt cacggcccaa ctcggccttc agcgaggctt gagcagccct gaccgcggcc
     1141 agggtgtcgt ggagatgaac cgggatacgg accgtgcgcg acttatcggc aatggcccgc
     1201 gtgatggcct gacggatcca ccaggtcgca tatgtggaga acttgaagcc cttgcgccag
     1261 tcgaacttct cgacggcgcg aatcaggccg aggttgcctt cctggatcag gtcgaggaag
     1321 tcaatcccgg aggtgttggc gtaacgacgt gcattggcca cgaccagacg gaggttggcg
     1381 gtcaagaagt catccttggc ctgggcaccg cgccgcgaaa ggcgacgcaa ttccatctcc
     1441 tcgcgcttgc cgctgtactc gcccttctcc aggcgctcgg cggcttcttc acccgcttcg
     1501 atcttctggg cgagctcaac ttcttgctcg gcggtgagca ggtcgtactc accaatggcg
     1561 gtgaggtact gggagacgag gtcggtcgtg agacggccgc gctcgtccgt cagcttcgaa
     1621 tgaagacggg ggcgtggctt ttcagttttc tcgatacgcg tttcagcagc ggccataaag
     1681 atctaagtcc tcctgggggc ccgttgggca gggatgcggt gatccccggg ccgcccaact
     1741 atgacacaag aagggcgttt tgtgaacccc cctcttttca acaatgttta gacgtgctgg
     1801 ggacaactgg agttcccgtc tcgccaacac aaagaaaaac gcatgcgggc cgctccacat
     1861 tccgcttttc accccgcatc ggcctcattc tcgatcccac tcggacccaa agaacatgcg
     1921 ggggttgatc acgaatctca gccatccccg cccctcgaca agcccttccc aggtgtccgc
     1981 cagaaggtcg atggcagcca ccccggcggt tctcatctcc acccggccgc ccgtgatttg
     2041 ctcggccaac atcgaccacg tcggctcatg gccgacgagc atgattcgct cctcgtcggt
     2101 ggcgatgccc tcagcgacgc cgaggactgt ccccgggccg gtgccgtaga gatcggactc
     2161 gactgtgatc gggcatttcc acgacccggc gcggacaact tcttctgctg tcgcccgggc
     2221 ccgagccgcg gacgaggtga tcacaagatc cgggacctcg tcccaagctg tgagcaccct
     2281 gcccatcaca gttgcgctcc gatggcccct cggggcaagg ggacggtcgt ggtcggagcc
     2341 gtaggaagca tcc
//
LOCUS       DBNHXU010000375         3817 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2135149, whole genome shotgun sequence.
ACCESSION   DBNHXU010000375 DBNHXU010000000
VERSION     DBNHXU010000375.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3817)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3817)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3817
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2135149"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..865)
                     /locus_tag="AC161X_09195"
     CDS             complement(<1..865)
                     /locus_tag="AC161X_09195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989769.1"
                     /translation="MVQASNVVEDAKDSPTRRPNRTGLVWLVAGLLIGAGLAVVFLDT
                     DIAPEPSTTTSTLGPQETRVVSIAGISEEIPGFPDGFMAIKRGEGLGLELLVWPESGD
                     PTVRSVPFGTSSPPDPVAFDLSGRLAATLIPAPPDEVTSILYAGVPEAAAIISLDVTG
                     YAWHDSVPEVLAYSTLQDGETKLWITRGNLASSELFAQAVGIGGGVVAVGEWGFAVQN
                     GDEVALFDFFGDMTQLGVGRILGSEPTGWLSVDDNGLTLVNVDGSRRVIPTGASSLLD
                     ATFSPDGTLIAG"
     gene            997..2340
                     /locus_tag="AC161X_09200"
     CDS             997..2340
                     /locus_tag="AC161X_09200"
                     /inference="COORDINATES: protein motif:HMM:NF024850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HIR4989770.1"
                     /translation="MKTKRGLKLLTVLAVLSLILAACGGSEGGETTTTAGSEATTTTA
                     ATAETTTTAAPDEGLGSVTVAAGEAIQIRSLEAISGDVSTLGIPNQNSTQLAIADYGQ
                     IQGHDVTFGTGLDDLCSADGGQAAASQIVADAQVVGVIGTSCSGAAAGAMPLISDAGL
                     VMISPSNTSPSLTSDLAGTAAENYFPGYYRTAHNDLYQGRAAAEFMVEQGITKAAAIH
                     DGDPYTDGLATAFADAFTELGGEVVIYTAVQKGDTDMVPVLTEVAGAAPEAIFFPIFP
                     PEGNFIVQQIAGVSGLEDVLLMAADGLLVDNFMELPESADMYFSGPDQRYGANANGVT
                     GKSADEVLAAYNETFGEAPTAAFWAHAYDATVMLLDAINTVAVDDGSGNLTIDRQALR
                     DQLNSVSGFAGLIGTLSCDDFGDCGATKITVVQNTDPSDIAAGKQNVVFEFVGAS"
     gene            2433..3536
                     /locus_tag="AC161X_09205"
     CDS             2433..3536
                     /locus_tag="AC161X_09205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020388729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HIR4989771.1"
                     /translation="MTTTTEIKPTKTRRFRFNWVDSFLWFIRIAAIITIAVGLFGSIQ
                     LFLAGEGPNAEQWRILVVSSISQGAMYGLIALGYSMVYGVLGFINFAHGEVFMAGTMI
                     GFVAANTLSQSGFWDAYPIPSILIVLVVAMGTSMLSAVLIEKIAYRRLRGAPRLIPLI
                     TSIGVSFFLQYAFAGLFGVGVRTYPALPGAMGGQVNILGVPVEVTKLMVIGVAILSSI
                     GLWYFVTRTKAGRAVRAVAEDKEIAGLMGINVDRTIVTTFAIGGAMAGVAALMWALLF
                     RSVDFTTGVLPGIKAFTAAVLGGVGNLPGAMLGGVLLGLFEGVGPLLILAGLGIPSVS
                     QLKDLVAFSALVLVLIFKPSGLLGEQLGSEDRV"
     gene            3533..>3817
                     /locus_tag="AC161X_09210"
     CDS             3533..>3817
                     /locus_tag="AC161X_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989772.1"
                     /translation="MSVPLSIKRGLIAGLSMSFVGGIGMVAVFNNKMLISPILAMGYA
                     TLFGLPLVIAWASTKREALEGFETEPPNVKDVLTGAIIGAVGGITTSLYVL"
BASE COUNT          714 a         1193 c         1153 g          757 t
ORIGIN      
        1 ggcccgcgat gagggtgccg tcgggggaaa aggtggcatc gagcaacgag ctcgcaccgg
       61 tggggatcac gcgacgcgat ccgtccacat tgaccagggt cagaccgttg tcatcgacag
      121 aaagccaccc ggtaggttcc gaaccgagga tgcgaccgac accaagctga gtcatgtcac
      181 cgaagaagtc gaagagggca acctcgtcac cgttctgaac ggcgaaaccc cattcaccca
      241 ccgcgacaac gccacctcca atgccgactg cctgcgcgaa aagctccgaa ctggcgagat
      301 ttccccgtgt aatccaaagt ttcgtctccc cgtcctgcaa cgtcgagtag gccagcacct
      361 ccgggaccga gtcatgccag gcatagccgg tgacatcgag cgaaatgatc gccgccgcct
      421 ctggtacccc ggcgtaaagg atgcttgtca cctcatccgg cggcgctgga atgagcgtgg
      481 ccgccaggcg tcctgaaagg tcgaacgcca ctggatcggg cggtgacgag gtcccaaagg
      541 gaaccgacct gaccgtggga tcaccgcttt cgggccaaac caagagttcg aggccgagac
      601 cctctcctcg cttgatggcc atgaatccat ccggaaaccc cgggatctcc tcactaatcc
      661 cggcaatcga caccacccgc gtctcctgtg ggcccaatgt cgaggtggtg gtcgacggtt
      721 ccggagcgat atcggtgtcg agaaacacaa ctgcgagccc ggccccgatc aggagtccgg
      781 cgaccaacca gaccaggccc gtgcgattcg gcctccgagt cggcgaatcc ttggcatctt
      841 ccacgacatt cgatgcttgg accacccttc cacggtacgc cgataacggg gtttgagggc
      901 tgctcagggt gccggaaccg aatttggggt ctaaagtcgc cccatacggc gcggcttcta
      961 gtcgtgccgc tctgaccaag tggaggaagg aaacacatga aaacaaagag aggcctcaag
     1021 cttctcacgg ttttggctgt cctttcgctc atcctggccg cctgtggcgg ctcggagggt
     1081 ggcgaaacga cgaccactgc tggtagtgag gcaactacta ccacggcagc gacagctgag
     1141 accacaacga cggcagcgcc agacgagggc ctcggctccg taactgtcgc cgccggagag
     1201 gccatccaaa tccgttcgct cgaagccatc agtggcgacg tgtcgacgct aggtatcccg
     1261 aatcagaact caactcaact cgccattgcc gactatggcc agatccaggg tcacgatgtg
     1321 acttttggaa ccggtctcga cgacttgtgc tccgctgacg gtgggcaggc agcagccagc
     1381 cagatcgtgg ctgacgcaca ggttgttggt gtgattggaa cctcgtgctc cggcgctgcc
     1441 gccggtgcga tgcctctgat ctcagatgcc ggtctggtga tgatctcacc gtccaatacc
     1501 tcgccttctc tgacctcaga cctggcgggc acggcggccg agaactactt ccccggctac
     1561 taccggaccg cccacaacga cctctaccag ggtcgtgccg cggccgagtt catggtggag
     1621 cagggcatca ccaaggccgc cgctatccat gacggcgacc cttacaccga tggtctggcc
     1681 accgccttcg ccgacgcctt cacggagttg ggtggagagg ttgtcattta caccgcggta
     1741 cagaagggcg acaccgacat ggtgcccgtg ctgaccgaag tggcgggtgc agcacccgag
     1801 gccatcttct tcccgatctt cccgcccgag ggcaacttca tcgttcagca gatcgctgga
     1861 gtatcaggcc tggaggatgt cctcctgatg gccgccgacg gtctcctggt cgacaacttc
     1921 atggaactcc cggagtcggc ggacatgtac ttctcaggtc ccgaccagcg ctatggcgcg
     1981 aatgccaatg gggtcaccgg gaagtcggct gacgaggtgc tcgctgccta caacgagacc
     2041 ttcggtgagg cgccgacggc tgcgttctgg gcacatgcct acgacgcaac agtcatgctg
     2101 ctcgatgcca tcaacaccgt ggcggtggac gacggcagtg gcaacctgac gatcgatcgt
     2161 caggcgctgc gtgaccagct gaacagcgtc tcaggcttcg caggtctgat cgggactctg
     2221 tcctgtgacg actttggtga ctgtggtgca accaagatca ccgttgtcca gaacaccgac
     2281 ccgtctgaca ttgcggcagg caagcagaac gtcgtgttcg agttcgtggg cgccagttga
     2341 tccgataatt cgtcttcgga cgaactgcta gtcagaaaag ccccggtctc tcggtaatga
     2401 gaccggggct ttctctatag tccccccacc tcgtgacgac gaccaccgag atcaagccaa
     2461 ccaagacccg acggtttcga ttcaactggg tcgactcatt tctctggttc atcaggattg
     2521 cggcgatcat caccatcgcg gttgggctgt tcggttcgat ccagctcttc ctggcgggtg
     2581 aaggcccgaa cgctgagcaa tggcggatcc tggtggtttc cagcatcagc caaggggcga
     2641 tgtatgggct gatcgccctc ggatattcga tggtctacgg cgtgctcgga ttcatcaact
     2701 tcgcccacgg cgaagtgttc atggccggga ccatgatcgg gttcgtcgcc gccaacaccc
     2761 tctctcagag cggtttctgg gacgcctacc cgattccatc gatcctgatc gtcctcgtcg
     2821 tagccatggg tacctcgatg ctcagcgctg tcctcatcga aaagatcgct taccgacggc
     2881 tccgaggtgc cccgcgcttg atcccgttga tcacctcgat cggggtgtcg ttcttcctcc
     2941 agtacgcctt cgcagggctg tttggggtgg gagttcgcac ctatcccgcc cttcccgggg
     3001 ccatgggcgg ccaggtcaac atcctggggg tccccgtcga agtcaccaag ctgatggtga
     3061 taggggtggc cattctctcc tcgatcgggc tctggtactt cgtgacgcgg accaaggcag
     3121 gccgcgcggt gcgggcggtg gccgaggaca aggaaatcgc cggccttatg gggataaacg
     3181 tcgatcgcac catcgtcacc acttttgcca tcggtggcgc catggccggg gtggccgccc
     3241 tgatgtgggc cctgttgttc cgcagcgtcg atttcaccac cggggtcctg cccgggatca
     3301 aagctttcac cgcagcggtg ctgggtggag tgggcaacct ccctggtgcc atgctcggtg
     3361 gggtcttgct cgggcttttt gagggggtcg ggcctttgct catcctggcc gggctcggaa
     3421 tcccgtcggt ctcccagctc aaggatctcg tggccttcag tgcgttggtc ctggttctga
     3481 tcttcaagcc ttcgggcctg ctcggggagc agctcggctc cgaggaccgg gtatgagtgt
     3541 gcccctcagc atcaagagag gcctgatcgc cggtctctcc atgagtttcg ttggggggat
     3601 cggcatggtg gcggtcttca acaacaagat gttgatctca cccatcctgg ccatggggta
     3661 cgccaccctt tttggcctcc cattggtgat tgcctgggcc tcgaccaaac gagaggcgct
     3721 cgaaggattc gagactgagc ccccgaatgt caaagacgtc ctcaccgggg ccatcatcgg
     3781 cgccgtcggt gggatcacaa cctcgctgta cgtccta
//
LOCUS       DBNHXU010000376         2983 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2731051, whole genome shotgun sequence.
ACCESSION   DBNHXU010000376 DBNHXU010000000
VERSION     DBNHXU010000376.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2983)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2983
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2731051"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(69..857)
                     /locus_tag="AC161X_09215"
     CDS             complement(69..857)
                     /locus_tag="AC161X_09215"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012387.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000413 - protein peptidyl-prolyl
                     isomerization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HIR4989773.1"
                     /translation="MTDRRRRQKEQRAAKQEAEKKQTARRELGRRLGTALIFGLLVIG
                     VLVLGSVFGDDEGDLPQGYEGFRNQPTACGAEQPAAEPVTQFTEPEPQTDITPTAAVT
                     ATLETSCGEIVIELDPAGYPETVNSFVFLAREGFFDGQVFHRIIADFQLFAGDPKADG
                     TGGPGYRLPDELPPDDFVYTPGVVAMDNNGKGTTGSQFFIVLSDDAQVLNPQFNGLGN
                     VTSGQETLDRIQAVDTARRPGSVEDSLPLETVYIETVTIDVTGS"
     gene            943..2175
                     /locus_tag="AC161X_09220"
     CDS             943..2175
                     /locus_tag="AC161X_09220"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014374381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009085 - L-lysine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HIR4989774.1"
                     /translation="MAIVVQKFGGTSLADSERIKAVADRVESTRRNGDDVVVVVSAMG
                     ETTDQLVALAAELSDDPSPREMDMLLSAGERISMALLAITLNSRGIPAVSYTGSQAGI
                     LTDSSHGSAKILDIQGTRVRDSLTEGRVVIVAGFQGVDPESKEVTTLGRGGSDATAVA
                     LAAALGADTVEVYTDVDGVFTADPRIVPDATKLAEVPFDEMLELSAGGAGVLMSRAVE
                     FGRRFDIPIHVRSSFHDGEGTWIRESAMEQAIVSGIAHDRSEAKVTVNRVPDRPGVAA
                     ALFGALAEAGVNVDMIVQNVSHDGSTDISFTVPRIHVVEAERISKHIAVDIGAQSVDV
                     DTDVAKVSLVGAGMKTHPGVAARVFEAMSAAGINIEMISTSAIRISCVIRGSQVEAAV
                     KALHAEFDPPTIFEEVGA"
     gene            2172..>2983
                     /locus_tag="AC161X_09225"
     CDS             2172..>2983
                     /locus_tag="AC161X_09225"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF011456.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004073 - aspartate-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HIR4989775.1"
                     /translation="MSDRVVAVVGATGAVGRTMLSILEHSDFPLGSVRLMASSRSAGT
                     VVESAWGEIVVEDLAQADPAGVDIALFSAGGARSREFAPSFAAAGAVVIDNSSAFRME
                     EAVPLVVAGVNDEAAHRHQGIIANPNCTTMTLMMVAGPLHRAAGLRSMNVASYQAVSG
                     SGQQGVSELIDQVDKLRPDLGALMTGGWADPGPVLFSRPIAFNVLPFAGSETDQGYTD
                     EEWKLVHESRKILDIPGLLVEPTCVRVPVLVGHAVVATLRFDREMGPGEAREL"
BASE COUNT          513 a          900 c         1003 g          567 t
ORIGIN      
        1 gctgtcgaat gggagtggag gtcaacggcc atgggtctcc tgcaccccga tgatggctgg
       61 tgtgaccgtc aagaaccggt tacatcgatg gtcacagtct cgatgtagac agtctcgagc
      121 gggaggctgt cctccacaga tccgggtcgt cgcgcggtgt cgaccgcttg gatccgatcg
      181 agggtctcct gaccggaagt cacgttgccc aggccgttga actgcgggtt gaggacttgg
      241 gcatcgtcgc tgaggacgat gaagaactgg ctgccggtcg ttcccttgcc gttgttgtcc
      301 atggccacca cgccgggtgt gtagacgaag tcatctggtg gcaactcgtc gggcaaccgg
      361 tagccgggcc caccggtgcc gtcggccttc gggtccccag cgaagagctg gaagtcggcg
      421 atgatccgat ggaagacctg cccgtcaaag aagccttcac gagcgagaaa gacgaaggag
      481 ttgacggtct cgggataacc ggcggggtcg agctcgatca cgatctcgcc acatgaggtc
      541 tcaagagtcg cagtgacggc agcagttggg gtgatatccg tctgcggctc cggctcggtg
      601 aattgggtca ccggttcggc cgccggctgc tcggccccac atgcggttgg ctggtttcgg
      661 aacccctcgt agccctgagg caggtcgcct tcatcgtcgc cgaagacgct gcccaacacc
      721 agaacgccga tcacaaggag cccgaagatc agagcagtgc ccagtctgcg acccagctca
      781 cgccttgcag tctgcttctt ttcggcctcc tgcttggcgg cgcgctgttc cttttgacgc
      841 cgtcttctgt cggtcatggc cggcgcgact ttaggcacga ccctccatca acctcctgcg
      901 gtgttcgtct tccgcggcga aaggctccaa actgtgttcg acatggcgat cgtggttcag
      961 aaatttggtg ggacgtcact ggctgattcg gagcgcatca aggcggttgc cgatcgggtg
     1021 gagtcgacgc ggagaaatgg cgacgacgtg gtggtggttg tctccgcaat gggagagacg
     1081 accgatcaac tggtggccct cgcggccgag ctgagcgatg acccatcccc acgagagatg
     1141 gacatgctgc tcagcgcggg ggaaaggatc tcaatggcgc tgctcgcaat cacgctgaac
     1201 tcgcggggga ttcctgcggt cagttacacc gggtcccagg ccggaatcct cacagactca
     1261 tcgcatggct cggcgaagat cctcgacatc caggggaccc gggtccgcga cagtctgacc
     1321 gaaggtcggg tcgtgatcgt ggccggattc cagggagtgg acccagaatc gaaggaagtg
     1381 acgaccttgg gccgcggcgg ctctgatgcc accgcagtgg ctctggcggc ggcccttggg
     1441 gcggacaccg tcgaggtcta caccgatgtc gatggggtct tcaccgctga cccgaggatc
     1501 gtgcccgacg ccaccaagct ggccgaggtg ccgttcgacg agatgctcga attgtccgcc
     1561 gggggtgccg gcgtcctcat gtctcgtgcc gtggagttcg ggcgccgctt cgacattccc
     1621 attcacgttc gctcatcctt tcacgacggc gaagggacct ggatacggga gtcggccatg
     1681 gagcaggcaa tcgtgagtgg catcgcccac gatcgctccg aagccaaagt gacggtgaac
     1741 cgtgtgcccg accgccccgg cgtcgccgcc gcattgttcg gcgctttggc cgaggccgga
     1801 gtaaatgtgg acatgatcgt tcagaatgtg tcccacgacg ggtccaccga catcagcttc
     1861 acggtgcccc ggatccatgt ggtcgaggcg gagcggatct ccaagcacat cgcagtcgac
     1921 atcggcgccc agtcggtcga tgtcgacacc gacgtcgcca aggtctcttt ggtgggggcc
     1981 gggatgaaga ctcatcccgg tgtcgccgca agggtcttcg aagccatgtc agcggctggc
     2041 atcaacatcg agatgatctc gacgtcagcg attcggatct catgcgtgat cagggggagt
     2101 caggtcgaag cggcggtgaa ggcactgcac gcggagttcg acccaccgac cattttcgag
     2161 gaggtgggag cgtgagcgat cgagtggttg ccgtggttgg agccaccggg gcggttggcc
     2221 gcaccatgct ctcgattctc gaacacagcg actttcccct gggttcggtc cggctcatgg
     2281 cctcctcgcg ttcggcgggg acggttgtgg agtcggcgtg gggtgaaatc gtcgtcgagg
     2341 acctggccca ggccgacccg gccggtgtcg acatcgcact tttctcggcg ggtggggcac
     2401 gttcccggga gtttgcgcct tcgttcgccg ccgctggagc ggtggtaatc gacaactcgt
     2461 cggcgttccg gatggaggag gcggtcccgc tggtcgtggc cggcgtcaac gatgaggccg
     2521 ctcacaggca ccaggggatc atcgccaatc ccaactgcac caccatgacc ttgatgatgg
     2581 tcgccggtcc gttgcaccga gccgccgggc tacggagcat gaatgtcgcc tcctaccagg
     2641 cagtctccgg ttcgggccaa cagggtgttt ccgagctgat cgaccaggtg gacaagctcc
     2701 ggcccgacct cggtgcactg atgacaggcg ggtgggcgga ccccggaccc gtccttttct
     2761 cccgccccat cgccttcaac gtgctgccat ttgccggctc cgagaccgac cagggctaca
     2821 cggacgagga gtggaagttg gtgcacgagt cgcggaagat cctcgacatc cccggactac
     2881 tagtggagcc aacctgtgtc cgggtgccgg tgctggtggg ccacgccgta gtggccacac
     2941 tccgtttcga ccgagagatg ggccctgggg aggcccggga gct
//
LOCUS       DBNHXU010000377         4482 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3722571, whole genome shotgun sequence.
ACCESSION   DBNHXU010000377 DBNHXU010000000
VERSION     DBNHXU010000377.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4482)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4482)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4482
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3722571"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1485
                     /locus_tag="AC161X_09230"
     CDS             <1..1485
                     /locus_tag="AC161X_09230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020377454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HIR4989776.1"
                     /translation="AVTFAATTVVISLLGMVLMNLGFVTGIAVACATVVAVTASASLT
                     LLPAMIGFAGERIEVTRWRGLIAALLLAVGLVGVAIAFQPLAIIGLSLAAVVIVASFA
                     IAPLRTEVPRRAQRPLKETVPYRWSRFIQHRPWLSAIAGVVILLTLAYPFLDQRLAFS
                     DEGNYPEETTTRQAYDLLAAGFGPGFNGPLLLAVEIPEGTDPAALEAVTEALAADPGV
                     AFVTPAIPNDPEAPTAALWRLFPTTAPQAEETTDLVIRLRDETLPEATSGSELDIAVS
                     GGVAAAVDFSEYLTDRLPTFIGVVLALSFLLLMVVFRSLLVPLKAVIMNLLSLAAAFG
                     VVTAIFQWGWGGGLLGIEPAPIEPFVPMMMFAIVFGLSMDYEVFLLSRIREEYLKSGD
                     SKESVADGLALTARVITAAAAIMVVVFGSFIGETDRIVKLFGVGLATAVLVDATIVRM
                     LLVPATMELLGDANWWLPKWLDRMLPKISVEGHALEVPAEPERV"
     gene            complement(1491..1844)
                     /locus_tag="AC161X_09235"
     CDS             complement(1491..1844)
                     /locus_tag="AC161X_09235"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR4989777.1"
                     /translation="MPKALLVCDTPWVLNEVLASLSTGGWEVEATDDPTEAATKAAEG
                     AVDAVIVDMQVQSMGGMAMIRDIKAAFEGVPRPRTVLLLDRSADQFLARRAGADAWLL
                     KPFQSSELRAALTPS"
     gene            1918..3144
                     /locus_tag="AC161X_09240"
     CDS             1918..3144
                     /locus_tag="AC161X_09240"
                     /inference="COORDINATES: protein motif:HMM:NF051402.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HIR4989778.1"
                     /translation="MRRFLLTVAVLLTLFPLSAVGADPEAGIDVIDVSGPLDASALEF
                     MRSSIELAAERGQVLAVLQIDSPAVLDGAALEALNATVQDPPLPVAIWVGPAPAVAYG
                     GIAQLVTDAEWRAISPGSYLGKPLPTIAGDDIAEGTIGEINAIPAEETGLDLQPTIRQ
                     YLQDLDGETFATADGPVVVETLKPFEDGVTLKTVTFLQPSLGTRFFRLAVIPEAAFFF
                     LVIGLTIVSFEFFAMGPGVAAGVASICLLLAGWGIVNLPVRWWALALAVFGWGLLTAA
                     YQKGGVIVLTILGALLLQIGGIWFVEGSGQIDARWFLILPSVLVVLFFFLLAMPTVQR
                     ARYSTQTIGRDSLIGETGVARVGLDPDGIVEVHGARWRATSHREAKVATGTQVVVTGV
                     DGLFLEVDRVPTEREN"
     gene            3237..3485
                     /locus_tag="AC161X_09245"
     CDS             3237..3485
                     /locus_tag="AC161X_09245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WhiB family transcriptional regulator"
                     /protein_id="HIR4989779.1"
                     /translation="MSHLDDWQLDALCRGNHAYLFFPPSTFERKDERERREMKAKAIC
                     GVCPVRQPCSEHAIQIREPFGIWGGLTEVERRVMPLVS"
     gene            3489..4193
                     /locus_tag="AC161X_09250"
     CDS             3489..4193
                     /locus_tag="AC161X_09250"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR4989780.1"
                     /translation="MAADPASHPVKPRPAASVLLVRDAADGPEVLMVERPARGYFGGL
                     HVFPGGGVDPLDNSALARQVVSGPDEDHGFRAAAVREVAEEVGMALTPEGVISAPVLR
                     GEALFESLHAAGLSLDGESLVLVSRWVTPSYAPVRFDTRFYVVGVEETPAVRLDSEEL
                     TSHLWASPGDLLAMHGRDEMELILPTLTHLRWLTRRNDVNDVLASATGADGRTLIEPR
                     EMEDGSLVPIHLPADQ"
     gene            4190..>4482
                     /locus_tag="AC161X_09255"
     CDS             4190..>4482
                     /locus_tag="AC161X_09255"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR4989781.1"
                     /translation="MIRIDRILANNAGPFTGPGTNTWLLDDGQGEVVIIDPGPVDSAH
                     FEAIQSAILGRSVIAVIVTHTHEDHAPMANPLALELGVPAYGYAPGPEFDPDL"
BASE COUNT          708 a         1411 c         1437 g          926 t
ORIGIN      
        1 gccgtcacct ttgcggccac gaccgtcgtc atctccttgc tcggcatggt gctgatgaat
       61 ctcggcttcg tgaccggtat cgcggttgcc tgcgccacag tggttgccgt taccgccagc
      121 gcttcgctca ccctcctccc ggccatgatc ggctttgccg gagagcgaat cgaggtcaca
      181 cggtggcgcg gcctcatcgc cgccctcctt ctcgccgtcg gcctggtcgg cgtggcgatt
      241 gctttccaac cgctggcaat catcgggctc tccctggccg cggtggtcat tgtggccagc
      301 ttcgccattg ccccattgcg cacagaggtg cctcgtcggg cccagcgtcc gctgaaggag
      361 acagtcccat accgctggag ccgtttcatc cagcatcgac catggttgtc cgcgatcgcc
      421 ggcgtggtca tcctgctgac acttgcctac ccgttcctcg atcagcgcct cgctttctcc
      481 gatgaaggca actatcccga ggagacgacg acccgtcagg cctacgacct cctggccgcc
      541 ggtttcggcc ccggtttcaa cggcccgttg ctgctcgccg tggagatccc tgagggcacc
      601 gatcctgccg cactggaggc tgtcaccgag gctctcgccg ccgatccagg ggtcgccttc
      661 gtaaccccag ccattcctaa cgatccggag gcaccgactg cggcgttgtg gcgattgttc
      721 ccgaccaccg caccccaggc cgaggagacc acagatctcg tgatcagact gcgagacgag
      781 acgctgcctg aggcgacttc aggcagcgaa ctcgacatcg ccgtgtccgg tggggtggcg
      841 gctgcggtcg acttctctga gtatctcacc gatcggttgc cgaccttcat cggcgtggtg
      901 cttgcactgt cgttcctcct gttaatggtg gtgttccgct cgctactggt gccgctcaaa
      961 gcggtgatca tgaacctgct ctccttggcc gcggcctttg gggtggtgac ggcgatcttc
     1021 cagtggggat ggggaggggg cttgcttggc atcgagccgg cgccgatcga gcccttcgtc
     1081 ccgatgatga tgttcgccat cgtcttcggt ctctcgatgg actacgaggt gttcctcctc
     1141 tcgaggatcc gtgaggagta cctgaagagt ggcgatagca aagaatcggt cgccgacgga
     1201 ctggccctca ccgcgagggt catcactgcg gctgcggcga tcatggtcgt cgttttcggc
     1261 agtttcatcg gcgagaccga ccggatcgtc aagctgttcg gagtgggcct cgccaccgca
     1321 gtgctcgtcg acgccacgat cgttcggatg ctgttggttc cggcgaccat ggagctactc
     1381 ggcgatgcca actggtggct gccgaaatgg ctcgatcgaa tgctgccgaa gatctcggtg
     1441 gagggccatg ccctcgaggt gcccgctgag cctgaaaggg tctgaccgga tcaggacggc
     1501 gttagggccg ctcggagctc cgaggactga aacggcttga gaagccaggc gtcggcgccg
     1561 gcccgcctgg cgaggaattg atctgctgat cggtcgagga ggagcacagt cctcgggcgg
     1621 ggcacgccct cgaaggcggc cttgatgtcg cggatcatgg ccattccgcc catgctttgc
     1681 acctgcatgt cgacaatcac cgcatcgacg gcaccctcgg cggccttggt ggcggcctca
     1741 gtgggatcgt cggtggcctc cacctcccag ccacccgttg acagcgaggc gagaacttcg
     1801 ttgagaaccc acggtgtgtc gcagacgaga agggccttgg gcatcggcgg tggacgttag
     1861 ccccggtggc gagggccccg gattccaggt gacgatcgcc tcctaacctc ccaagtcgtg
     1921 cgtcggttcc tgctcacagt ggcggtcctg ttgaccctct tccccctttc ggctgtcggc
     1981 gccgacccgg aggccgggat agatgtcatt gacgtcagcg ggccgctcga tgcgtccgcc
     2041 ctggaattca tgaggtcttc aatcgaattg gcggccgagc ggggccaggt gctggcggtg
     2101 ctccagatcg actcaccggc ggtactggac ggcgctgcgt tggaagcgct gaatgccacc
     2161 gttcaggatc cgccgcttcc tgttgcaatc tgggtcggcc ccgcacccgc tgttgcgtac
     2221 ggcggtatcg cccaactcgt gacggacgcg gagtggaggg caatttcacc aggctcctac
     2281 ctggggaagc ccttgccgac cattgccgga gacgacatcg cggaaggcac gatcggagaa
     2341 atcaacgcca tccccgccga ggaaaccggg ctcgatctcc agcccacgat ccgccaatac
     2401 ctccaggatc tcgatggtga gacgttcgcc acggccgacg gcccggtggt agtggagacc
     2461 ctgaagcctt tcgaggacgg agtcactctg aagaccgtca ctttccttca acccagtctg
     2521 gggacccgct tcttccgttt ggcggtgatc ccggaggccg ccttcttctt cctggtgatc
     2581 ggcctcacca tcgtttcgtt cgagttcttc gccatggggc cgggggttgc agccggagtg
     2641 gcatcgatct gcctgctcct tgccggatgg gggatcgtca atctcccggt gcgttggtgg
     2701 gcgctggcgc tcgcggtgtt cggctgggga ttgctcaccg ccgcctatca gaaaggtgga
     2761 gtcatcgtgc tcaccatcct cggggcgctg ctgctccaga tcgggggcat ctggttcgtc
     2821 gagggctcag gtcagatcga tgcccgctgg ttccttatcc ttccctcggt gttggtcgtg
     2881 ctgttcttct tcctgttggc gatgcccacg gtgcaacgcg cccgctattc gactcagacc
     2941 atcggacgtg actccctgat cggcgagacc ggcgtcgccc gggtcggcct cgacccggat
     3001 gggatcgtcg aggtgcacgg tgctcgttgg cgggcgacca gtcatcggga ggcaaaggtg
     3061 gcgaccggca cccaggtggt ggtcaccggg gtcgacgggc tcttcctcga agtggatcga
     3121 gtcccaacag agcgcgaaaa ctgattttca gacctggcaa aatcttcctt gtgatcgacc
     3181 cgcgttctga gtaggtttcg acccggttgt cgcttcaaaa tacgggagga gtcgaattgt
     3241 cccacttgga cgattggcag cttgacgcgc tgtgccgtgg gaaccacgcc tatctcttct
     3301 ttcccccatc gaccttcgaa cgcaaggacg agcgggagcg cagagagatg aaggcaaagg
     3361 ccatctgtgg ggtctgtcca gtgagacaac cttgctccga acatgcgata cagatccgtg
     3421 agccgttcgg gatctggggt ggattgaccg aggtcgagag aagggtcatg cccctggtca
     3481 gctgacgtat ggcagctgac cccgcttcac accccgttaa gccccgcccg gcagcgagcg
     3541 tcctcctggt tcgcgatgcc gccgatgggc ccgaagttct catggtggag cggcctgctc
     3601 gcgggtattt cggtgggctc catgtctttc ccggcggtgg cgtggatccc ttggataaca
     3661 gcgcactggc gcgccaggtg gtgagcgggc ccgacgagga ccacggattc cgggctgccg
     3721 cggtgaggga agtggccgag gaggtcggga tggccctgac tcctgagggt gtcatctctg
     3781 ctcccgttct ccgcggagag gcgcttttcg aatcgttgca cgccgcggga ttgtcgctcg
     3841 acggtgaaag cctggtactc gtgtcacgtt gggtgactcc gtcctacgcc ccggttcgct
     3901 ttgacacgag gttctacgtg gttggggtcg aggagacgcc agctgtcagg ctcgacagcg
     3961 aggagttgac cagccacctt tgggcgtcgc cgggggactt gctcgccatg cacggtcgcg
     4021 atgagatgga gctgatcctc cctacgctga ctcatctgcg atggttgacc cgtaggaacg
     4081 acgtgaatga cgtgctcgct tcagcaacgg gcgccgacgg gcggacactg attgagcccc
     4141 gcgaaatgga ggatgggtcc ttggtcccga tccacttacc ggcagaccag tgatccggat
     4201 cgaccgcatc ctggccaaca acgccggccc tttcaccggg cccggcacca acacctggct
     4261 tctggatgac ggtcagggtg aggtggtgat catcgatccc ggcccggtcg actctgccca
     4321 cttcgaggca attcagtcag ccattctcgg gcgttcggtg atcgcggtca tcgtcaccca
     4381 cactcacgaa gatcatgctc caatggccaa cccgcttgcg ctcgagctcg gggttcctgc
     4441 ttatggctac gcacccgggc ctgaattcga tccagatctg ag
//
LOCUS       DBNHXU010000378         1588 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2534105, whole genome shotgun sequence.
ACCESSION   DBNHXU010000378 DBNHXU010000000
VERSION     DBNHXU010000378.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1588)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1588
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2534105"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..164)
                     /locus_tag="AC161X_09260"
     CDS             complement(<1..164)
                     /locus_tag="AC161X_09260"
                     /inference="COORDINATES: protein motif:HMM:NF044960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989782.1"
                     /translation="MPYLEAPESLGEVFKGEPGYRADQLRDWLYKTPVLRPESMTNLP
                     GDIRQRLEGHL"
     gene            complement(168..488)
                     /locus_tag="AC161X_09265"
     CDS             complement(168..488)
                     /locus_tag="AC161X_09265"
                     /inference="COORDINATES: protein motif:HMM:NF025873.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HIR4989783.1"
                     /translation="MKRLAKIVGFVAGAAALAWAMRDRFISVATSREPEPPTFRTGAP
                     LTVSPDIDVIDGIGPVFAVRLKSAGIDNAAKLAAASPDRVAEAASVSAARARSWIEQA
                     KSID"
     gene            536..1552
                     /locus_tag="AC161X_09270"
     CDS             536..1552
                     /locus_tag="AC161X_09270"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012122415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HIR4989784.1"
                     /translation="MKVDYYFPPSPPEGAGAAAREAAGLGYGGFFTAETQYDPFMPLV
                     LAGVEAPALELGTAIAVAFPRSPTVVAMTAWDLARLSGGKFLLGLGTQVRAHIVRRFA
                     STWDQPGPRLRDYVLALRAIWEAWQNSTPLKYEGEFYNLSLMTPFFDPGPIPNPDIPV
                     YIAGVGPYLSRLAGELCDGFHVHPFHTTRYLDRVVLPGITEGATRASRSLDDVELATT
                     VFVMTGETDAEIETAMSGVRQQISFYASTPTYKPVLESNDWYFGEQLTAMSKRGQWGE
                     MAAVVPDEAVLEVGVAAPIDRLGTAIRERYGDRVQRIGFYAIGSGLDSDREALTEVIR
                     QIRL"
BASE COUNT          266 a          497 c          519 g          306 t
ORIGIN      
        1 agatgcccct cgaggcgttg acggatgtcg ccagggaggt tcgtcatgga ttcggggcgt
       61 aggaccgggg tcttgtagag ccagtcccgc aattggtcag cccgatagcc aggctcacct
      121 ttgaaaacct ctcccaggct ctcgggagct tcgaggtaag gcacctgtca gtcgatcgac
      181 ttggcttgct cgatccagga tcgagcccgt gccgcgctga cactcgctgc ctcggcgacc
      241 cggtcgggtg acgccgcagc aagctttgct gcgttgtcga tgccggcgct cttcaatcgc
      301 accgcgaaca ccgggccgat gccatcgatg acgtcgatgt caggtgagac cgtcaaaggc
      361 gctccggtcc ggaatgtcgg cggctctggc tcgcgtgagg tggcaaccga gatgaaccgg
      421 tcccgcatcg cccaggcgag cgccgcggca ccggctacga atccgacgat cttggcgagt
      481 cgtttcatca aggagccaag gctacccatt ccggcctgcc tctacgctgg tggacatgaa
      541 ggtggactat tactttccac ccagtcctcc ggagggggca ggggcggctg cccgtgaggc
      601 ggccggtctc ggctacgggg gctttttcac cgcagagacc caatatgacc ccttcatgcc
      661 gctggtgctc gccggtgtcg aagcgcccgc cctcgagctc ggcaccgcta tcgccgttgc
      721 ctttccccgt tctcccaccg tggtggcgat gaccgcgtgg gatctcgccc gactttccgg
      781 gggaaagttc ctgctcgggc tggggacgca ggtccgcgca cacattgttc gccgcttcgc
      841 atcaacgtgg gaccagccag gcccgcggct tcgggactac gtcttggcgt tgcgggccat
      901 ctgggaagcg tggcagaact cgacgcctct caagtatgaa ggggagttct acaacctttc
      961 attgatgact ccgttcttcg atcccgggcc tatccccaat ccggacatcc cggtctacat
     1021 cgccggtgtc ggcccctatc tgagccggct ggcgggcgag ttgtgcgacg ggtttcacgt
     1081 ccaccccttc cacaccaccc ggtatctgga tcgggtcgtc ctacccggga tcaccgaagg
     1141 ggcgactcgg gccagtcgct cgctggatga tgtcgagctg gcgacaaccg tgtttgtgat
     1201 gaccggggag accgatgccg agatcgagac ggcgatgagt ggggttcgac agcagatctc
     1261 gttctatgcc tcgaccccta cctacaaacc ggtgctggag tcgaacgact ggtacttcgg
     1321 cgagcaactg accgcgatgt ccaagcgggg ccaatggggg gagatggcgg cggtggtgcc
     1381 tgacgaggct gtgctcgagg taggggtggc cgccccgatc gaccgcctcg gcacagcgat
     1441 cagggagcgc tacggcgatc gggtgcagcg gatcggcttc tatgccatcg gctcgggtct
     1501 ggacagcgat cgcgaggctt tgaccgaggt gatcaggcag atccggctct gaatgctggc
     1561 gaactcggtc agaacaccaa tgcactcg
//
LOCUS       DBNHXU010000379         5887 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2731514, whole genome shotgun sequence.
ACCESSION   DBNHXU010000379 DBNHXU010000000
VERSION     DBNHXU010000379.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5887)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5887)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5887
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2731514"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..257)
                     /locus_tag="AC161X_09275"
     CDS             complement(<1..257)
                     /locus_tag="AC161X_09275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012181200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HIR4989785.1"
                     /translation="MASQEEVFDSPSGWVSKHIDEYVETDGQKGYEWRNGAPTLLLTT
                     RGRKTGKLRRTALIFGVDGDRYVLVASVGGAAKNPAWYLNLV"
     gene            286..711
                     /locus_tag="AC161X_09280"
     CDS             286..711
                     /locus_tag="AC161X_09280"
                     /inference="COORDINATES: protein motif:HMM:NF020397.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR4989786.1"
                     /translation="MEKTATDPTSFIRSLPTDVRTDIEALDALISEVMHGYSRVMWEG
                     KFWGGTEQNIIGYGDLAYSRPGKETVHWFIVGLASQKDHLSIYVSAADEDGYLVKKYQ
                     DRLGKVKIGSAVITVKKADDLDLPVLRELVAAAADLTSG"
     gene            complement(752..2779)
                     /gene="ligA"
                     /locus_tag="AC161X_09285"
     CDS             complement(752..2779)
                     /gene="ligA"
                     /locus_tag="AC161X_09285"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008413167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HIR4989787.1"
                     /translation="MPDVRTQIEDLRSQVNEHLYRYHVLDDPTISDAQFDALMADLKG
                     LEEEHPELVTPDSPTQRVGAPIGELFQPVRHLRPLFSLDNATSREDLETWEQRLERQL
                     GGPPTGYVAELKIDGLAVVLTYRDGVLATGATRGDGVTGEDITANLRTIKSVPLKLLD
                     KKPPSILEVRGEVYMPEAAFDELNRKQAEAGDRMFANPRNAAAGSVRQKDPAMTAARN
                     LAIWVYQLGLMEGGPDFQSHADTIHYLQEVGFRTNPASQELESIDAVLGYVARAEKDR
                     HQTGYQTDGVVIKVNALAEQDALGFTSRAPRWAIAYKFPAEEQVTTLRDIWINIGRTG
                     AATPFAVLEPVFVGGATVGMATLHNADQVALKDVRIGDQVIVRRAGDVIPEVVGPVLS
                     ARTGKEKKWKMPAKCPFCGNPIVRVEGEAVARCTGGYTCPSRVREWLFHFASRGGMDI
                     EHMGYKTIDLLLTEGMISDPADIFIFDPDELLGREGWGAISVANLKKAIAAAKDRPAS
                     RLLTALGIRHVGGTVARLLVRHFGGLPGLMAATEEQISAVEGIGPVIANGVATWAADP
                     DNQALVAKLGAAGVRLEDEVDRSASSDLLAGAIFVVSGTVPGFSREEAQAAIEQRGGK
                     AASSVSRKTTALIVGESPGASKSRKAEELGIPILDGDGFKRLLEEGLSALG"
     gene            complement(2807..3856)
                     /gene="mnmA"
                     /locus_tag="AC161X_09290"
     CDS             complement(2807..3856)
                     /gene="mnmA"
                     /locus_tag="AC161X_09290"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR00420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HIR4989788.1"
                     /translation="MRALVAMSGGVDSSVAAALMKQAGHDVVGVTLKMWSGPNGEAPT
                     AGCCTVSDAEDARRVAGQLGIPYYVLDYTTEFRDGVVDRFISDYTSGRTPNPCIECNR
                     TVKFDRLLRRLDDFESDFLVTGHHARSRFDGKAWSLHRGADRAKDQSYVLSMLAQREL
                     SRTRFPVGELTKTEVRAKASDLGLRTALKPESMDICFVGRRDYRDFLARTAPGSFTAG
                     PMIDTDGHELGEHDGISGFTVGQRRGLGVAVGEPRFVVRIEPATSTVVLGRRDELRVG
                     GVDLSEVIWTAGIGGSGAVMAQYRAHGDPVPAVLEGDRLLFTDFQEGVSPGQTIAFYD
                     DDRVLGGGLIDRTFL"
     gene            complement(3853..4986)
                     /locus_tag="AC161X_09295"
     CDS             complement(3853..4986)
                     /locus_tag="AC161X_09295"
                     /inference="COORDINATES: protein motif:HMM:NF012488.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase family protein"
                     /protein_id="HIR4989789.1"
                     /translation="MIYLDHAATTPMRPGVWDAMAPYADIIYGNSSGIHGVSRRAKDA
                     LEEAREKIASAIGADTLEIVLTSGGTESDNLAIKGAVFSGSPRRGMVTVATEHEAVLE
                     TSDFVRRLGIPVTVVGVDIEGMVDLDQLLAAIDRETAIVSTMLVNNETGAIQAVADIA
                     RAVKSHNPDVLVHTDAVQAFGSEPIDVAALGVDLMSLAAHKFGGPKGAGILYVREGTL
                     LEPVQHGGGQELGRRSGTHDVGRAVGMAEAMDLALGDREDFRHRVRQIRDDFEKRLIA
                     GIEGLEVNTPEERRSPHHLNVRFPGVLNETLLVRMDLAGVAASAGSACQSGATEVSHV
                     LEAMGLTPNEARESIRFSFGWPSTIAEAEEAAEIVTALVGQLR"
     gene            5006..5479
                     /locus_tag="AC161X_09300"
     CDS             5006..5479
                     /locus_tag="AC161X_09300"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005488 - binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4989790.1"
                     /translation="MRGLFESEIPSSSLLHQLIVDIPAPPERVWAELADLGSHHEWMA
                     DAEEIRFTDQQRSGLGTRMLVPTRVGPFRTTDLMTVTEWDEGRSMSVEHVGAVSGSGR
                     FALEPIETGTRLIWSEQLRFPWWLGGSLGALVAGPVLKRIWKGNLGRLAARVTGP"
     gene            complement(5466..>5887)
                     /locus_tag="AC161X_09305"
     CDS             complement(5466..>5887)
                     /locus_tag="AC161X_09305"
                     /inference="COORDINATES: protein motif:HMM:NF012596.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HIR4989791.1"
                     /translation="RYLAEDALVGQPEVLLGIIPGAGGTQRLGRLVGYQRAKEMILTG
                     RQVGAEEALTFGLADKVAPSGEILELALADAVAWATKATRAIAAAKKAMHRGFGTPVD
                     EGLMVERDAFQVAFGSEDAREGVAAFIEKRAPEFKGR"
BASE COUNT         1051 a         1949 c         1830 g         1057 t
ORIGIN      
        1 acaaggttca ggtaccaggc gggattcttg gcggcgccgc cgaccgacgc caccagaacg
       61 tagcgatcac cgtcgacgcc aaagatcaag gcggtacgac gcaactttcc ggtcttgcgg
      121 ccgcgggtgg tgagcagcaa cgtgggcgct ccgttgcgcc attcatagcc cttttggccg
      181 tcggtctcta cgtattcgtc gatgtgtttc gacacccacc cgctgggact gtcgaatact
      241 tcttcttgcg atgccattgt ccctccggct ttacttcgcc aggtcatgga gaagacggct
      301 accgacccga caagcttcat ccgatccttg ccgacggatg tccgcaccga catcgaagca
      361 ctcgacgcct tgatcagcga agtgatgcat ggttactcca gagtgatgtg ggagggcaaa
      421 ttctggggtg gcaccgagca gaacatcatc ggctacggcg atctcgccta ctcgcgtcct
      481 ggcaaagaga cagttcattg gttcatcgtg gggctcgcct cgcagaagga ccacctcagc
      541 atctacgtca gcgccgcgga tgaagatggc tatctggtca agaaatacca agaccggctg
      601 ggcaaggtca agatcggaag tgcggtgatc acggtcaaga aggccgacga cctcgacctg
      661 ccggtgcttc gagagctggt cgcggccgct gccgacctca catctggcta gaccccgaca
      721 gagcggattg ctgacaaccc agggctatga atcagcccag agccgacaag ccctcctcca
      781 gcagcctctt gaacccgtcc ccgtccagga tggggatacc cagctcctcg gccttccgcg
      841 acttggaggc gccgggtgac tcaccgacga tcaacgccgt ggtctttcta gagaccgagc
      901 tggccgcctt gccgccccgc tgctcgatgg cagcctgagc ctcctcacga ctgaacccgg
      961 ggaccgtccc cgataccaca aagatggccc cagccagcaa gtcggaggag gcgctgcggt
     1021 cgacctcgtc ttcgagacgg acgcctgctg cccctaattt ggcaaccagc gcctggttgt
     1081 ccggatcggc ggcccaggtc gcgacaccat tggcgatcac cggcccgata ccctcgaccg
     1141 ccgagatctg ctcctcggtc gccgccatca atcctggaag cccaccgaag tgacggacca
     1201 ggagtctggc caccgtcccc ccgacgtgtc taatgcccag cgcggtcagc agccgggagg
     1261 cgggccggtc cttggcagcc gcgatcgctt tcttcagatt ggcgaccgag atcgctcccc
     1321 agccctcgcg gcccagcaat tcatccgggt cgaaaatgaa gatgtcggcc gggtccgaga
     1381 tcatcccctc ggtgaggaga aggtcgatgg tcttgtatcc catgtgctca atgtccatcc
     1441 cgccccggga ggcgaagtgg aacagccact cccggacccg cgaggggcag gtgtagcccc
     1501 cggtgcacct cgcaaccgcc tcgccctcga cacgaacgat ggggtttcca cagaacgggc
     1561 acttcgcggg catcttccat ttcttctcct tgccggtgcg ggcggagagc accggcccga
     1621 ccacctcggg gatcacgtcg ccggcgcggc ggacgatcac ctgatccccg attcggacat
     1681 ccttgagagc tacctgatct gcgttatgga gggtggccat tccaaccgtg gcgccaccga
     1741 cgaacaccgg ctcgaggacc gcgaagggag ttgcggcgcc ggtacggccg atgttgatcc
     1801 agatgtcgcg aagcgtggtg acctgctcct ctgcgggaaa cttataggcg atggcccacc
     1861 gcggcgcccg agaggtgaaa ccgagggcgt cctgctccgc caacgcattg acctttatca
     1921 cgacgccgtc ggtctgatac ccggtctggt ggcgatcctt ctcggctctg gccacgtagc
     1981 cgagcacggc gtcgatgctc tctaactcct ggctggcagg gttcgttcgg aaccccactt
     2041 cctggagata gtggatggtg tcggcgtgtg actggaagtc aggcccgcct tccatcaacc
     2101 cgagttgata cacccatatg gcgaggttgc gggccgcggt catcgccggg tccttctgac
     2161 gcaccgaccc ggcggcggca ttgcgtggat tggcgaacat ccggtcaccg gcctcggcct
     2221 gcttccgatt gagctcgtcg aacgccgcct cgggcatata gacctcgccc cgaacttcga
     2281 ggatggatgg tggcttcttg tcgagaagct tcagtggaac cgacttgatg gtgcgcaggt
     2341 tggcggtgat gtcctcaccg gtcaccccgt cgcccctagt ggccccggtt gcgagcactc
     2401 cgtcccggta ggtgagaaca acggccaagc cgtcgatctt gagctcggcg acatatccgg
     2461 ttggaggtcc cccgagctga cgttccaatc gctgctccca ggtctccagg tcctcgcgcg
     2521 aggtcgcatt gtccagcgag aacagggggc gcaggtggcg caccggctga aacaattccc
     2581 cgatcggcgc tcccacccgc tgggtcgggc tgtccggtgt gaccagctcc gggtgttcct
     2641 cctcgagtcc cttcagatcc gccatgaggg cgtcgaactg ggcgtcggaa atcgtcggat
     2701 cgtcaaggac gtgatatcga tacaagtgct cgttgacctg ggaacggagg tcttcgatct
     2761 gggtccggac gtcaggcatc agcgagccga cgttaccgac cggctgtcac agaaaagtcc
     2821 gatcgatcaa acccccaccg agcacccggt catcgtcgta gaaggcaatg gtttggccgg
     2881 gggacacccc ttcctgaaag tcagtgaaca ggaggcgatc accttcgaga acggccggga
     2941 ccgggtcacc atgggcccgg tactgagcca tcaccgcgcc tgatccgcca atgccggctg
     3001 tccagatgac ctccgagagg tcgacaccgc ccaccctcag ctcatcacgt cggccgagga
     3061 caacggtcga agtggcaggc tcgatgcgca ccacgaatcg gggctcgccg accgcaacgc
     3121 cgaggccgcg gcgctgcccg acggtgaacc cgctgatccc atcgtgctcc cccagttcgt
     3181 gcccgtcggt gtcgatcatc ggaccggcgg tgaacgagcc cggggcggtc cgggcgagga
     3241 agtcccgata gtcgcggcgt ccgacgaagc agatgtccat cgactccggc ttcagcgcgg
     3301 tccgcaatcc gagatcagac gccttggccc gcacttcggt cttggttagc tccccgaccg
     3361 ggaaccgggt cctggacaat tcccgctggg cgagcatcga gaggacatag gactggtcct
     3421 tggcccgatc ggctccgcga tgaaggctcc aggccttgcc gtcgaatcgg ctccgggcgt
     3481 gatggccggt cacgaggaaa tcgctttcga agtcatcgag acggcgaagc agccggtcga
     3541 atttcaccgt ccgattgcac tcgatgcacg ggtttggagt gcggcctgag gtgtagtcac
     3601 tgatgaagcg gtcaaccacc ccatcccgga actcggtggt gtagtccagg acgtaatacg
     3661 ggatcccgag ttggccggcc acccgccgag catcctcggc atcggacacg gtgcaacacc
     3721 cggctgttgg ggcctcgcca ttgggaccgg accacatctt cagggtcacc cccaccacat
     3781 cgtggccggc ctgtttcatg agggctgcag ccaccgacga atcgactcca ccggacatcg
     3841 ccaccagcgc cctcatctga gttgccccac cagagccgtc acgatctcgg cggcctcctc
     3901 ggcctctgca atggtgctcg gccagccaaa gctgaaacgg atgctctccc tggcctcgtt
     3961 gggagtcagc cccatcgcct cgaggacgtg agagacctcg gtggctcccg actgacatgc
     4021 ggatccggct gatgccgcga cgccagcgag gtccatgcgg accagaagcg tttcgttgag
     4081 cactccggga aatcgaacat tgagatgatg gggcgagcgt cgctcctcgg gagtgttgac
     4141 ctccaaaccc tcgattccgg cgatcagtcg cttctcgaag tcgtcacgga tctggcgaac
     4201 tcgatgccga aaatcttcac ggtcgcccag ggccaggtcc atggcctcag ccatccccac
     4261 tgcccgtccg acatcatggg tcccggagcg cctgccgagc tcctgacctc caccgtgctg
     4321 taccggttcc aggagtgtcc cttcacggac atacaagatt cccgccccct tggggcctcc
     4381 gaacttgtgg gcggccagtg acatcaagtc cacaccgagc gccgcaacgt cgatcggctc
     4441 ggagccgaag gcctggacgg cgtcggtatg caccaggacg tcgggattgt ggctcttgac
     4501 tgcacgggcg atatcggcca cggcctggat cgcgccggtc tcgttgttga ccagcatcgt
     4561 cgagacgatc gccgtctccc tgtcgattgc cgccagcaac tggtcgagat caaccatgcc
     4621 ctctatgtcg acgccgacca cggtgaccgg aatcccgagc cgcctcacga aatcactggt
     4681 ctccaggacc gcttcatgct cggtagccac ggtgaccatc ccgcgacgcg ggcttccact
     4741 gaaaaccgcc cccttgatgg cgagattgtc cgactcggtg cccccggagg tgaggacgat
     4801 ctccaaagtg tcggcgccga tggccgatgc gatcttctcc cgagcttcct ccagggcgtc
     4861 tttggcgcgg cgggataccc cgtggatacc ggaggagttg ccatagatga tgtcggcgta
     4921 gggcgccatg gcgtcccaga ctccggggcg catcggtgtg gtggcggcgt ggtcgaggta
     4981 gatcacagca ccggattctc gcaggatgcg gggcctgttc gagtcagaga taccctcgtc
     5041 ctccttgttg caccaactga tcgtcgacat cccggcgcca cccgaacggg tctgggccga
     5101 gctcgccgac cttggctcac atcatgaatg gatggcagat gcggaagaga tccgattcac
     5161 ggatcagcaa cgcagcggtt tgggcacccg gatgctggtc ccgacccggg tagggccgtt
     5221 tcgaaccacc gacctgatga cggtgaccga gtgggatgag ggccggtcga tgtccgtcga
     5281 gcacgtgggc gcggtctccg gctccggccg attcgctctc gagcccatag agaccgggac
     5341 ccggctcatc tggtctgagc agctccgatt cccatggtgg ctgggcggtt cactgggggc
     5401 cttggtggca ggcccggtgc tgaagcggat ctggaagggg aatctgggcc gtctcgcggc
     5461 tcgcgtcacc ggcccttgaa ttccggcgcc cgcttctcga taaaggcggc gacaccctcg
     5521 cgagcgtcct ccgacccgaa ggcgacctga aaggcgtcac gctcgaccat gagaccctcg
     5581 tccaccggcg ttccaaatcc acgatgcatc gccttcttgg cggcggcgat ggcccgggtc
     5641 gccttggttg cccaagccac ggcgtcggcg agggccaact cgaggatctc gccggacggg
     5701 gcgaccttgt cggcgagacc gaacgtcagg gcttcctcgg cgcccacctg ccgcccggta
     5761 aggatcatct ccttggctcg ttggtagcca acgaggcgac ccagccgctg ggtccccccg
     5821 gcaccgggga tgatcccgag caggacctcg ggctggccga ccagggcatc ctcggcgagg
     5881 tagcgaa
//
LOCUS       DBNHXU010000380         2874 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4916496, whole genome shotgun sequence.
ACCESSION   DBNHXU010000380 DBNHXU010000000
VERSION     DBNHXU010000380.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2874)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2874)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2874
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4916496"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..940)
                     /locus_tag="AC161X_09310"
     CDS             complement(59..940)
                     /locus_tag="AC161X_09310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989792.1"
                     /translation="MSEVVGGATRRPPRFRFGRWLGVLPFFVFSGLFLIGPALILAYR
                     SLEAPDGSLTLDNYAALTDPNVMAAYGMSIRISLLTAFLGGLFGFALAWAVSMGGLGR
                     SSRSGVSTFSGVASNFAGVPLAAAFIFTLGRIGIITDLFEWMGVDIYGSGFSLYTFWG
                     LTIVYLYFQLPLMLLVILPAIDGLKKDWREAAENLGATPRQYWRRVAFPVLLPSILGT
                     MILLFGNAFGAHATAYALTGGSIPLATLLIGQQISGDVLNNPGLGYAVAMGMVFIMAG
                     SILIYSILQRRSERWLG"
     gene            complement(951..2117)
                     /locus_tag="AC161X_09315"
     CDS             complement(951..2117)
                     /locus_tag="AC161X_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012190011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4989793.1"
                     /translation="MKKRYAMVAVLALLIAACGSGTETTTTAGGTATTADGGTATTAA
                     AGGMEELIAAAQAEGGLTTIALPRDWCGYGAVIDGFSAKYGITVNELDPGAGSAEELE
                     AIDANKDNPGPQAPDVIDVGLAFGPQAKEAGQIQPYKVATWDEIPDGAKDAEGYWYGD
                     YYGVLALETNMSEVTTAPTEYADLLGADFAGKVALSGDPLTSNAAALSVWAAGLATGA
                     TGEEAATAGLEFFAQLNDAGNFVPIDGDSRTVGIGETPVLIDWSYNSIANAAALAGNP
                     EIATNVPADGRMAGVYVQAISAYAPHPNAAKLWMEYLYSDEGQLGWATGYCHPIRFEA
                     MSAAGTIPDDILANLPSTEGALFPTIEEIEAAKAVIVEGWPTVVGVTVAESPSG"
     gene            complement(2199..>2874)
                     /locus_tag="AC161X_09320"
     CDS             complement(2199..>2874)
                     /locus_tag="AC161X_09320"
                     /inference="COORDINATES: protein motif:HMM:NF012705.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HIR4989794.1"
                     /translation="RHHLVDQVFMRLDTRTRARITKLIQDMATGMIEFSTIFERQDGV
                     LDDEEQVLAYCHRVIGLPALFVMETLLGPVSKDHHRDALEVSELIQLANITRDIEKDL
                     RRGVAYHPALRAYLGSDNGSAVAVAAARRDLIHLATRRAPSFRRLVDAVGLPRLSPAR
                     AAAVLMVLFTGRHYRDCAINAGMPFRSGPRRKSTLVLTSIPAAVSPRWADRVMIRVET
                     DLLATA"
BASE COUNT          544 a          890 c          950 g          490 t
ORIGIN      
        1 ggaaccgcgt cgaccgatgg cgcctctgcc gcggttggaa gcggagtcgt ggcaatcgtc
       61 accccaacca cctttcagag cgtcgctgca ggatcgagta gatcaggatg ctcccggcca
      121 tgatgaagac catccccatc gcaaccgcat agccaagccc cggattgttg agcacatcac
      181 cggagatctg ctggccgatg aggagcgtgg ccagagggat gctgccgccg gtgagggcgt
      241 aggcggtggc gtgtgcgcca aaggcattgc cgaagagaag gatcatggtg cccagaatcg
      301 atgggagcag caccggaaac gcgacccgac gccagtattg gcggggggtg gcaccgaggt
      361 tctcggccgc ctcacgccag tccttcttca gaccgtcgat ggcggggagg atcacaagga
      421 gcatgagcgg aagctggaaa tagagataga cgatggtcag cccccagaag gtgtagaggc
      481 tgaatccgct gccatagatg tcgacgccca tccactcgaa gaggtcggtg atgatcccga
      541 tccggcccaa ggtgaagatg aaggcggccg ccagcgggac gccggcgaaa ttagaggcca
      601 cccctgagaa ggtgctgaca ccagaacggg aagatcggcc gagaccaccc atggacaccg
      661 cccatgccaa ggcgaatccg aaaagaccac ccaggaacgc ggtgagcaag ctgatcctga
      721 tcgacatccc ataggcggcc atgacgttgg ggtcggttag cgcggcgtag ttgtcgaggg
      781 tcaggctgcc gtcgggagcc tccaggctgc gatacgccag gatcagggcg gggccgatga
      841 ggaacagccc ggaaaagaca aagaagggca ggacgccgag ccagcgcccg aaacggaatc
      901 gtggggggcg ccgggtggcg ccccccacga cttccgacaa tgcgtcttgg ctagccgctt
      961 gggctctccg cgacggtgac acccaccacg gtgggccatc cctcgacgat gactgccttg
     1021 gcagcctcga tctcctcgat cgtcgggaag agggcacctt cggtgctggg gaggttggcc
     1081 agtatgtcgt cgggaatcgt cccggcggca ctcatcgcct cgaaccgaat cgggtgacag
     1141 tacccagtcg cccaacccaa ttggccttcg tcggagtaca ggtactccat ccacaacttg
     1201 gcagcattgg gatgaggcgc ataggcgctg atcgcctgca catagacacc ggccatccga
     1261 ccatccgcgg ggacgttcgt tgcgatttcc gggttacccg ccagggctgc cgcgttggcg
     1321 atcgagttgt acgaccagtc gatcagaacc ggggtctccc cgattccaac cgtgcgtgag
     1381 tcgccgtcga ttggcacaaa gttgcctgcg tcgttcagct gcgcgaagaa ctcgagtcct
     1441 gccgtcgcag cctcttcacc ggttgccccg gtggcgagac cggcggccca caccgacagt
     1501 gcggccgcat tggaggtcaa cgggtcgcca gaaagcgcaa ccttgccggc aaaatcggca
     1561 cccaacagat cggcgtactc ggtgggagcg gtggtcactt cggacatgtt ggtttcgaga
     1621 gccaacaccc cgtagtagtc gccgtaccag tagccctctg catccttggc accgtccggg
     1681 atttcatccc aggtggccac cttgtagggc tgaatctgac cagcctcttt cgcctgcggt
     1741 ccgaaggcga ggccaacgtc gatgacgtcc ggagcctgcg gtcccgggtt gtccttgttg
     1801 gcgtcaatcg cctccagctc ctcagccgaa ccggcgccgg ggtcgagctc attgaccgtg
     1861 atcccgtact ttgcgctgaa gccgtcgatg acggctccat acccgcacca gtcgcggggc
     1921 agggcgatcg tggtcagccc gccttctgcc tgagccgcgg cgatcaactc ttccattccg
     1981 ccggcagctg ccgttgtggc ggtacctcca tcggctgttg tggctgttcc gccggccgtg
     2041 gttgtcgttt cggttcccga gccgcacgct gcgatcagca gtgccagcac ggcaaccatt
     2101 gcatatctct ttttcatctt gctccttggt tccatgacca accgggcgga gcctacccct
     2161 acgaagtgac cggagtcgga acgaacggga aacggacgtc aggcagtggc gaggaggtcg
     2221 gtttcgacgc ggatcatcac ccgatcggcc catcgagggg agaccgcagc ggggatcgag
     2281 gtcagcacca gagtcgactt acgacgcgga ccggatctga atggcatgcc ggcgttgatg
     2341 gcgcagtcgc ggtagtgacg gccggtgaag agcaccatca gcaccgccgc ggcccgggcc
     2401 gggctgagac ggggaaggcc gaccgcgtcg acgagacgac ggaacgaggg cgcccgtctg
     2461 gtggcgaggt ggatcaggtc gcggcgagcg gcggccacgg cgacagcgct cccgttgtcg
     2521 gacccgaggt aggcccgaag cgctgggtgg taggcgactc cgcgtcgcag gtctttctcg
     2581 atgtcgcggg tgatgttggc caactggatc agctccgaca cctcgagggc atcgcggtgg
     2641 tgatcctttg agaccggccc gagcagggtc tccatcacga atagggccgg cagtccgatc
     2701 acccgatggc agtaggccag cacctgctct tcgtcgtcga gcaccccgtc ctggcgctcg
     2761 aagatggtcg agaactcgat catgcccgtc gccatgtcct ggatcagctt tgtgatccgg
     2821 gcccgggtcc gggtgtcgag cctcatgaac acctggtcga cgaggtgatg cctt
//
LOCUS       DBNHXU010000381         4129 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_550261, whole genome shotgun sequence.
ACCESSION   DBNHXU010000381 DBNHXU010000000
VERSION     DBNHXU010000381.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4129)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4129)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4129
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_550261"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..1053)
                     /gene="mraY"
                     /locus_tag="AC161X_09325"
     CDS             complement(1..1053)
                     /gene="mraY"
                     /locus_tag="AC161X_09325"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014376006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HIR4989795.1"
                     /translation="MISLLIAAAVGLLVSIFGTPYAIRFFRRREIGQFIQEEIVGMHG
                     HKQGTPTMGGVVFVSAAIIGYFVSHIRLLGPSGSFDFEFQPVRAGGLLAVFALSGMAV
                     VGLLDDWTKFSNKRSLGLSKSAKFGGQLAIAALFAWGASMAGVVTEISFVRPLGIDLG
                     AFFVIWVLIMLTGAANGVNLADGMDGLAGGSSALVVASYTVITFWQFRNPQVYESVDP
                     LELAIISAAMFGAILGFLWWNAPPAKIIMGDVGSQAIGGLLAALALLSSTNLLLVVLG
                     AIFVAETISVILQVASFRIFGKRIFRMTPIHYHFDLGGWPETTVVIRFWILTGIGVAL
                     GLGFFYGDFLRLEGLL"
     gene            complement(1050..2537)
                     /locus_tag="AC161X_09330"
     CDS             complement(1050..2537)
                     /locus_tag="AC161X_09330"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001124.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HIR4989796.1"
                     /translation="MPARGKKLVDLAVVVGGVTHGDPEVTVTDVTHDSRHAGAGTMYV
                     AVRGSHFDGHEFIPNAVAAGSSAVCVDHFTASGVSELEVRDTREVMGHLAASVHDDPS
                     GSVAVVGVTGTNGKTTVTHFIESITASAGRTPGLIGTVHTRVGDRTLSSERTTPEATD
                     FQRLLAEMRDLGADVIAAEISSHALELGRVSATRFAVAAFTNLSQDHLDFHGDMDAYR
                     AAKERLFQEYEVGAAVINIGDPVGGELASRVRMPVLRVGPGGELSAGEVRTSFEGTSF
                     EMILGGQSRQVTAPLIGMFNVENALVAAGCCLQLGLSVDEVVAGLGGLGPVPGRFELV
                     SGSFPVRVVVDYAHTPEGIHQAISVARDLAFGRVIAVFGAGGDRDREKRPLMGRAAIE
                     ADLSVLTSDNPRSEDPEAIIGEVMSGIEGSGVVVEPDRRRAVELAIRAASPGDVVLIL
                     GKGHETGQEIGERVLPFDDKLVAAEVIDRMARSSDSEGGSGSMAR"
     gene            complement(2521..>4129)
                     /locus_tag="AC161X_09335"
     CDS             complement(2521..>4129)
                     /locus_tag="AC161X_09335"
                     /EC_number="3.4.16.4"
                     /inference="COORDINATES: protein motif:HMM:NF013100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_function: GO:0009002 - serine-type D-Ala-D-Ala
                     carboxypeptidase activity [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="peptidoglycan D,D-transpeptidase FtsI family
                     protein"
                     /protein_id="HIR4989797.1"
                     /translation="SRELPPQRGKIYDRNGDLLAMTVETQSLYAVPGQVEDPVWVAQQ
                     VGGVLGVDSDLLRDRLASDRDFVYLKRQVEADLADEVMGLEIKGVYSLPEPSRVYPAG
                     QVASHVTGFVDIDGNGQEGLEYMYDDSLRGVEGHALFERDLEGRPIPQGISEITPAVP
                     GVDLNTTIDLPLQYQAQEACLSAVERTGAKSCWVVVLSVETGEVLAMTGAPAFDPQTR
                     ENVDPRCVDDPDPVKCLTFSNFIVRGLYEPGSTEKLITVAAAIEEGEVDDTTVIPDVA
                     DVLELREDACRSADDTTYGCYRDFDEHDTSDLTVAEIFTQSSNVGSILVAEKLGQERL
                     IDYIEAFGLGAKTGIDYSVEAPGLLNFAAGCETCWASAAIGYSVAVTPLQMAAAYAAI
                     GNDGVWTTPHIVSTTTDVDGNTEVAGFETRRVVSPETAVLMRNMLASVVDEGTGHQAT
                     IEGYRVGGKTGTANILGDDGRYTEVTRASFVGMAPIDDPQVVVAVLVDSPSWDYRTGG
                     ASAAPVFAEVMEEALHRLGVTPDASQG"
BASE COUNT          798 a         1358 c         1289 g          684 t
ORIGIN      
        1 tcacaacaaa ccctccaggc gcaagaagtc gccgtagaag aagccgagac cgagagccac
       61 gccgattccg gtgaggatcc agaacctgat caccaccgtg gtctccggcc agccgccgag
      121 gtcgaagtga tagtggattg gggtcatccg gaagatgcgt ttgccgaaaa tccggaacga
      181 ggccacctga aggatcaccg agatcgtctc ggccacgaag atcgccccaa ggacgaccaa
      241 caggaggttg gtgctggaga gaagggccag agcggcgaga aggccgccga ttgcctgcga
      301 accgacgtcc cccatgatga tcttcgccgg tggcgcgttc caccagagaa agcccagaat
      361 cgccccgaac atggcggcgg agatgatcgc cagttccagt gggtcgaccg attcgtagac
      421 ctgggggttt ctgaactgcc agaaagtgat gaccgtgtag gacgccacca ccagtgccga
      481 cgatcctcca gcaagcccgt ccatgccgtc ggcgaggttc acgccgttgg cggcaccggt
      541 gagcatgatc aggacccaga tcacgaagaa ggcgccgaga tcgattccca ggggccgcac
      601 gaaggagatc tcggtcacta cacctgccat ggaggccccc caggcgaaga gggcggcaat
      661 cgccagttgg cctccgaact tggccgactt ggaaagcccg aggcttcgct tgttggagaa
      721 cttggtccag tcgtcgagca ggccaaccac ggccatcccg gacaaagcga aaacggcgag
      781 caagcctccg gcacggaccg gttggaattc gaaatcgaag ctccccgagg gccccagaag
      841 gcggatatgg gacacgaagt acccgatgat cgccgccgac acgaacacca cgcctcccat
      901 ggtcggtgtg ccctgcttgt gcccgtgcat cccgacgatt tcctcttgga tgaactggcc
      961 gatctcgcgc cgtcggaaga agcgaatggc atacggcgtg ccgaagatgg acaccaatag
     1021 gccaacggcg gcagcgatga gcagcgagat caccgggcca tgctccctga accgccctct
     1081 gaatctgagg atctcgccat ccggtcgatc acctcggccg ccaccaactt gtcgtcgaag
     1141 ggaaggacgc gctccccaat ttcctggccg gtctcgtgcc ccttgcccag gatcaggacg
     1201 acgtcgccgg gcgatgccgc ccggattgcc agctcgacag ccctccgacg atcaggctcc
     1261 acgaccacgc cggagccctc gatacccgac atcacttcgc cgatgatcgc ttcggggtcc
     1321 tcagatctcg ggttgtcgct ggtcaagacg gacaggtcgg cttcaatcgc cgcccgcccc
     1381 atcaggggcc gcttctcccg gtcgcggtcg cctccggccc cgaaaaccgc gatcacccga
     1441 ccgaacgcca ggtctctggc caccgaaatc gcttggtgga tcccctcggg ggtgtgtgcg
     1501 tagtccacca ccacccgcac cgggaatgag cccgaaacca actcgaagcg cccggggacc
     1561 gggcccagtc cgccgagccc ggcgaccacc tcgtcgaccg agaggccgag ttgcagacag
     1621 cagccggccg cgaccagggc gttctcgacg ttgaacatgc cgatcaacgg ggccgtgacc
     1681 tggcggcttt gcccgcccag gatcatttcg aagctggtgc cctcgaagga ggtgcgaact
     1741 tcgccggcac tcagctcgcc gccggggccg acccggagga ctggcattcg aactctggag
     1801 gcaagttcgc ctccgacagg atcgcctatg ttgattaccg cggcgcccac ctcgtactcc
     1861 tgaaagaggc gctctttggc ggctcggtag gcatccatat ccccgtggaa gtcgaggtgg
     1921 tcctgcgaga ggttggtgaa agcagcgacg gcaaacctcg tggccgagac ccggcccaac
     1981 tcgagggcat gggaagagat ctccgccgcg atgacgtcgg cacccaggtc acgcatctcg
     2041 gcgaggagcc gctggaaatc ggtggcctcg ggtgtcgtgc gctccgagga aagggtgcgg
     2101 tcgcccaccc gggtgtggac cgttccgatg aggccggggg tgcggcctgc gctggcagtg
     2161 atcgactcga tgaagtgagt gaccgtggtc ttgccgttgg tcccggtgac ccccaccacc
     2221 gcgaccgagc cggaaggatc gtcgtgaacc gatgcggcga ggtggcccat cacctctcgg
     2281 gtgtcgcgta cctcgagctc ggatacccca ctcgccgtga aatggtcgac acagaccgcg
     2341 ctcgaccctg cggcgaccgc attgggaatg aattcgtggc cgtcgaagtg ggagccccgg
     2401 accgccacgt acatggtccc cgccccggca tggcgtgaat cgtgggtgac gtcggtaacg
     2461 gtgacctccg ggtcaccatg agtcacccct cccaccacca ccgccagatc caccaacttc
     2521 ttacccctgg ctggcatctg gggtcactcc gagacgatgg agggcctctt ccatgacctc
     2581 ggcgaacacc ggagcagccg aggcgccccc ggtgcgatag tcccaggaag gcgagtcgac
     2641 gagcacagca accacgacct gcggatcgtc aatgggtgcc atcccgacaa aggaggcccg
     2701 cgtgacctcg gtgtagcggc cgtcgtctcc aagaatgttg gccgtcccgg tcttgccacc
     2761 gaccctgtag ccctcgatgg tcgcctgatg gccggtgcct tcgtcgacca cgctggcgag
     2821 catgttcctc atcaacacag ccgtctccgg agagacgact cgacgggtct cgaaaccggc
     2881 gacttcggtg tttccatcga catcggtcgt cgtcgaaacg atgtgtggtg tcgtccacac
     2941 gccgtcgttg ccaatagccg cataggcagc agccatctgc aaaggggtca cagccacgga
     3001 gtagccgatg gccgccgagg cccaacaggt ctcacaccct gctgcgaagt tgagcaggcc
     3061 tggcgcctcg acggagtagt cgatcccggt cttcgccccg aggccgaacg cttcgatgta
     3121 gtcgataagg cgctcctgac cgagcttctc ggctaccaag atcgagccga cattggacga
     3181 ttgggtgaag atctcggcca cagtcaggtc ggatgtgtca tgctcatcga agtcccgata
     3241 acagccgtaa gtggtgtcat cggctgaacg acaggcgtct tctcgtagtt ccagcacgtc
     3301 ggcgacgtca gggatgactg tggtgtcatc gacctcgccc tcctcgatgg ctgcagccac
     3361 ggtgatcagc ttctcggtgg atcccggctc gtagagcccc cgcacgatga agttggaaaa
     3421 ggtgaggcat ttcaccgggt ccgggtcgtc gacacagcgg gggtccacgt tctcgcgggt
     3481 ctggggatcg aaggcaggcg ctccggtcat ggccaagacc tcacccgtct cgaccgagag
     3541 caccaccacc cagcacgact tggctccggt gcgttccacg gcgctgagac aggcctcctg
     3601 ggcctgatac tgcagtggaa ggtcgatcgt ggtgttgagg tccactcccg gcaccgcagg
     3661 cgtgatctca ctgatcccct gggggatggg gcgcccttcc agatcgcgtt cgaagagggc
     3721 gtgcccctcc acgccccgca gggagtcgtc gtacatgtac tccagacctt cctgcccgtt
     3781 gccatcgatg tccacgaagc cggtgacgtg gcttgcgacc tggccggcgg gatagacccg
     3841 gctcggttcg ggcagggagt agacaccttt gatctccaac cccatgacct cgtctgcgag
     3901 gtcggcctcg acctggcgct tcagatagac gaaatcgcgg tcggacgcca gccggtcgcg
     3961 gaggagatca gaatccacac cgaggacacc tcccacctgc tgagccaccc aaacagggtc
     4021 ctcgacttga cccggcacgg catagaggct ctgggtctcc acggtcatcg ccaacaggtc
     4081 accgttgcgg tcgtagatct tccctctctg gggcggaagc tcacgggac
//
LOCUS       DBNHXU010000382         2444 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_154302, whole genome shotgun sequence.
ACCESSION   DBNHXU010000382 DBNHXU010000000
VERSION     DBNHXU010000382.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2444)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2444)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2444
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_154302"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..493
                     /locus_tag="AC161X_09340"
     CDS             170..493
                     /locus_tag="AC161X_09340"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_216458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:0016075 - rRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HIR4989798.1"
                     /translation="MMDLPMRGEVWWCELPQISRRPVVVLSRNAAIPRLRRALVAPCT
                     TTVRGLQSEVVLEAGEDPIPQRSAVNLDSVESVSIGALTTRLGRLSAERMHQVCSALA
                     VAVDC"
     gene            564..959
                     /locus_tag="AC161X_09345"
     CDS             564..959
                     /locus_tag="AC161X_09345"
                     /inference="COORDINATES: protein motif:HMM:NF012257.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HIR4989799.1"
                     /translation="MDASRLESIPLFADLSRKDLKAVARHADEVDLPSGAKIVEQGRL
                     AYELFVVESGTADVLIDDQVIAQIGPGDVVGEIGVLKTHTRTATVVATSPVTAIVIYG
                     PELTALTESLPGVRAELERLIVERTGSTS"
     gene            1010..1696
                     /locus_tag="AC161X_09350"
     CDS             1010..1696
                     /locus_tag="AC161X_09350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989800.1"
                     /translation="MKSSRLIALVLALFLIAACGGASSGSSTTAPRDVVTTGGAAATT
                     VPGDATTVPSEDDPGVASLEDMPAECLQAFTDFLQAIEPYVENVDFENSTMADLEAVF
                     TEMEPIITPFEEETINCPDLDMSEEESLAAMREMAERDAPGTVAYFAWISEFMVEGGA
                     GGAEISGDCETDIATLQGYVDQGGTISDLTMGELAAVGELMSALGTECSSERFQEWLT
                     QEDVAAWSGG"
     gene            complement(1706..2227)
                     /locus_tag="AC161X_09355"
     CDS             complement(1706..2227)
                     /locus_tag="AC161X_09355"
                     /inference="COORDINATES: protein motif:HMM:NF015513.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HIR4989801.1"
                     /translation="MEEPQLSLTEYAVLGVLAEGPTHGFAISRDLAPKGSVGRVLTVR
                     RPLTYRALDRLVSVGLATPVHSERGDGGPQRLVHKATPAGKRRLKRWLGEPVWHVRDM
                     RIEFQLKLVLLQRSGSSPLALIEKQRRLLGPTLVALDIQSSDPPDLLELWRQHNAAAT
                     DGYLKALEDIYTT"
BASE COUNT          464 a          726 c          784 g          470 t
ORIGIN      
        1 gtttgcagat gaacgatgcg gccctgattg acgcggcatt gaccgctctg ttggacgctc
       61 accagacgga ggagatcaac gccgcctacc tggcatacga cggtcacccg ctcaatgagc
      121 ccgacgagtg gggcgacctc gaatcgtttc gtgccgcagc tggctcgtcg tgatggacct
      181 tcctatgagg ggagaggtct ggtggtgcga gttgccacag atctcacgcc gcccggtcgt
      241 ggtcctgtcc cgaaacgcgg cgattcccag gttgcgtagg gccctggtgg ctccctgcac
      301 tacaaccgtc cgtgggcttc agagcgaagt cgttctcgag gccggcgaag acccgatccc
      361 acaacgttcg gcggtcaacc tggactcagt cgaaagtgtg tccattggcg cattgacaac
      421 acgcctgggt cgactcagcg ccgagcggat gcatcaggtc tgctcggcgc tggccgtcgc
      481 cgtcgactgc tgatcaccta gcccggcggt cccaacccga ggggagctac cctccgactt
      541 ctcggacgga aagtgccaga acgatggatg caagtcggct cgaatcgatt cccctcttcg
      601 ccgacttatc ccgcaaggat ctcaaggctg ttgcccgtca cgccgacgag gtcgacctcc
      661 cgagcggggc gaagatcgtc gagcagggtc ggcttgccta cgagctcttt gtggttgaga
      721 gcgggaccgc cgacgtattg atcgacgatc aggtgattgc ccagattggc cccggggacg
      781 tagtcggcga gatcggcgtc ttgaagaccc acacccgtac tgcgactgtc gttgccacat
      841 caccagtcac cgcgatcgtg atctatggac ccgagctgac ggcgttaacg gaatccctgc
      901 caggggtcag agcagagctc gagcgtctga tagtcgagcg caccggctcg accagctgac
      961 gaaccccagc ggtgtagggt ctcgccccta cgacttggtg gaggttcatg tgaagagttc
     1021 ccgcttgatc gccttggtgt tggccctatt cctcattgca gcttgcggag gtgccagctc
     1081 gggctcaagc acaaccgctc cacgagacgt cgttaccacc gggggagcgg cggcgaccac
     1141 tgttcctggc gacgccacca ccgttcccag cgaagacgat cccggagtgg ccagtctcga
     1201 agatatgccg gccgagtgtc ttcaggcctt tactgatttc ctgcaggcga tcgagcccta
     1261 cgttgagaac gtcgacttcg agaactcgac catggcggat ctcgaggcag tgttcaccga
     1321 gatggagccg ataatcaccc ccttcgaaga agagaccatc aactgccccg acctcgacat
     1381 gtctgaggaa gagagtctcg cagcgatgag ggaaatggcc gagcgtgacg ctcccggcac
     1441 ggtcgcgtac ttcgcctgga tctcggaatt catggtcgaa ggcggcgcag gaggtgcgga
     1501 gatctctggt gactgcgaga ccgacatcgc gaccctccag ggatatgtcg atcagggtgg
     1561 gaccatctcc gacctgacca tgggagagct agccgcggta ggcgagctga tgtccgccct
     1621 tggcaccgag tgctcatctg agcgctttca ggagtggctc actcaggagg acgtcgctgc
     1681 atggtcgggc ggctagaagc catcgtcagg tggtgtagat gtcctcgagc gctttgagat
     1741 agccgtcggt agcagcggcg ttgtgctgtc gccacaactc gagaaggtct ggcggatcgc
     1801 tcgactggat gtcgagggcg accagggttg gtccaagtag ccgtcgttgc ttctcgatga
     1861 gagcgagcgg tgaagacccg gagcgctgca gcaagaccag tttgagttga aactcgatgc
     1921 gcatgtcgcg gacgtgccag acgggctctc cgagccacct cttcaatcga cgtttgcccg
     1981 caggcgtcgc cttgtgcacc aggcgctgtg gcccgccatc accacgctcg gagtggacag
     2041 gcgtggcaag accaacggac accaatcggt cgagggcgcg ataggtcagt ggtcgccgca
     2101 cggtgagcac ccggcccacg gaccctttcg gagccagatc gcgggagatg gcgaagccat
     2161 gagtcggtcc ttcggcgagg actccgagga ctgcgtattc cgttagggaa agctgtggtt
     2221 cttccacggt gcagaaaggg tagatacctc aggccgcatg acgtattctc cctgcgacta
     2281 ccaactagtc acgatgagag taatggaggc ctgattcatg cgcagatatc gactggcttt
     2341 gctggtcgca ttcgtcctca cgatcgcggc ttgtggttca tcgagtggag acaccaccgc
     2401 ggaggcgggg ttcgagggcg agctactggt ctctgccgca gcgt
//
LOCUS       DBNHXU010000383         1915 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4123146, whole genome shotgun sequence.
ACCESSION   DBNHXU010000383 DBNHXU010000000
VERSION     DBNHXU010000383.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1915)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1915
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4123146"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="AC161X_09360"
     CDS             complement(<1..873)
                     /locus_tag="AC161X_09360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HIR4989802.1"
                     /translation="MPVDRRVRAASVATALTLLVALFSVDYTVKPGDTLGAIARDNNV
                     SVSDLAAANNISNPNLIRAGQVLVIPSKDRTHVVSSGESLYTIAKKYGTTVAALIKAN
                     KLDNPNLIRPDQKLVVPGSAGGSGSSGGSGGSTGGSDSNGSNQKPDGNSARSGRYHIV
                     KKGETLEQIAKAEGVSVEAITTANGITNHVIYTGTRLFLDGPGHTSRGSSESGTYTVQ
                     RGDRLVDIANRYGTSVSKIASTNGISNINLIRAGRSLKVPAGGAWVCPVKGASFFNDW
                     GFPRAGGTRFHEGND"
     gene            complement(919..>1915)
                     /gene="rlmN"
                     /locus_tag="AC161X_09365"
     CDS             complement(919..>1915)
                     /gene="rlmN"
                     /locus_tag="AC161X_09365"
                     /EC_number="2.1.1.192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015441818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016433 - rRNA (adenine) methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0030488 - tRNA methylation [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dual-specificity RNA methyltransferase RlmN"
                     /protein_id="HIR4989803.1"
                     /translation="GEPGYRADQLRDWLYKTPVLRPESMTNLPGDIRQRLEGHLWPFE
                     VELEQFADKGRTRKWLFRAPDGAAIESVLMGYPRRATLCISSQAGCALGCTFCATGQF
                     GFERNLEAGEIVAQVAFAQAFLRQIGMPGVPDHLTNIVFMGMGEPLANYPRVREAIRR
                     MIEFMGLSARSITVSTVGIVPGIRMLADEPWPVYLAISLHAADDELRSRLVPINKRYP
                     LEELEAAAAEYLEKKRRRLSIEWTMMDGVNDTAEQAEKLARIALRLRAHVNLIALNPT
                     PLTQEKAPKGTVIRAFAGMLEGRGVNVTIRDTRGRDIDAACGQLRVFSGKRGLGG"
BASE COUNT          338 a          603 c          586 g          388 t
ORIGIN      
        1 gtcgttgcct tcatggaacc tggttcctcc ggccctgggg aagccccaat cattgaagaa
       61 gctggcgcct ttgaccgggc acacccatgc ccctcccgcc ggaaccttga gacttcgacc
      121 tgcccgaatc aggttgatgt tcgatattcc attggttgag gcgatcttcg agaccgacgt
      181 gccatagcga ttggcgatat ccactaggcg gtctccgcgc tgcacggtgt aggtgcccga
      241 ttcgctgctg ccccgggatg tgtgccccgg cccatccagg aagagcctgg tgccggtgta
      301 gatgacgtga ttggtgatcc cgttcgccgt agtgattgcc tcgacgctga caccctctgc
      361 cttggcgatc tgctcgaggg tctcgccctt cttgacgatg tgatatcgac cggagcgggc
      421 gctgtttccg tccggcttct gattggagcc gttgctgtcg gagcctcccg tggatccacc
      481 cgagccgccg ctgctccctg aacctccggc ggatcccggc acgaccagct tctgatcggg
      541 acggatcagg ttggggttgt cgagtttgtt ggccttgatc agcgcggcga cagtcgtccc
      601 gtacttcttg gcgatcgtgt acaggctctc gccgctcgac acgacatgcg tgcgatcctt
      661 gctcggtatc accagcacct gaccggcgcg aatcaaattc gggtttgaga tgttattggc
      721 ggcagccagg tccgacaccg agacgttgtt gtccctggcg atagcgccaa gcgtgtcgcc
      781 gggtttcacg gtgtagtcaa ccgagaagag ggcgacgaga agagtgagcg ccgtggctac
      841 cgaagcagcc cggacgcgac gatcgaccgg catgaaggca cagctccttc agcccctgaa
      901 ccgctccgcc cgaccaccct accccccgag accgcgtttt ccagaaaaaa ctcggagctg
      961 tccacaggcg gcatcgatgt cccggcccct ggtgtcgcga atggtgacgt ttaccccgcg
     1021 accttcgagc attccggcaa aagccctgat cacagtgcct tttggggcct tttcctgggt
     1081 caggggagtg gggttgaggg cgatgagatt tacatgggcg cgcaaccgca atgcgatgcg
     1141 ggccagtttc tcggcctgtt cggccgtgtc gttgaccccg tccatcatgg tccactcgat
     1201 cgaaagccgg cgacgcttct tctcgaggta ttcggccgcc gcggcctcca attcttccaa
     1261 cgggtacctc ttgttgatgg gcaccaggcg gcttctcaac tcatcgtcgg ctgcgtgcag
     1321 gctgatcgcc aggtacaccg gccacggttc gtccgccagc atccggatcc cggggacgat
     1381 gcccacagtg gagacagtga tcgaccgagc cgaaaggccc atgaactcga tcatccgacg
     1441 gatcgcctcc ctgacccgtg ggtagttggc gaggggctca cccatcccca tgaagacgat
     1501 gttggtgagg tgatctggga ctccaggcat gccgatctgg cgcaggaatg cctgggcgaa
     1561 ggcgacctga gccacgatct ctccggcttc gaggttgcgc tcaaatccga actgcccggt
     1621 tgcacagaag gtgcagccga gagcacagcc cgcctggctc gaaatgcaaa gcgtcgctcg
     1681 acgcggataa cccatcagca cggattcgat ggcggcaccg tcgggtgccc gaaacagcca
     1741 cttgcgggtt cggcccttgt cggcgaactg ctccaattcg acttcaaacg gccacagatg
     1801 cccctcgagg cgttgacgga tgtccccagg gaggttcgtc atggattcgg ggcgtaggac
     1861 cggggtcttg tagagccagt cccgcaattg gtcagcccga taaccaggct caccc
//
LOCUS       DBNHXU010000384         5723 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3725892, whole genome shotgun sequence.
ACCESSION   DBNHXU010000384 DBNHXU010000000
VERSION     DBNHXU010000384.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 5723)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 5723)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5723
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3725892"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..384)
                     /locus_tag="AC161X_09370"
     CDS             complement(<1..384)
                     /locus_tag="AC161X_09370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HIR4989804.1"
                     /translation="MVTFLAAAATAAAAALVVIAVHSAYRRRQKVAPVEAPWQSQAGL
                     DVTPTQFWLASLGAGVVTYLLVYAVTSLPVISAMPAFVVATLPRAYFGRKRGRRLAEV
                     QEAWPDGLRDLISSVRSGASLPTAIE"
     gene            complement(378..1652)
                     /locus_tag="AC161X_09375"
     CDS             complement(378..1652)
                     /locus_tag="AC161X_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TadA family conjugal transfer-associated ATPase"
                     /protein_id="HIR4989805.1"
                     /translation="MTTDAYQEIRRRALERIDGAGIDPAEESGPLMKLLEEAVDDYQR
                     LAHLGEGRSLANPEETVRRLYRSVSAHGPFGDLLEREDVEEIFIEGDSVTYLEAGGWL
                     RGLETPTSEEENRQLIERLIATTDRRLDASNPIAQARILDGTARLTAVIPPVADRLSA
                     TIRRYALRRETMDSLVGLASLTPAAASFLSSAMRASASVIVSGPPGAGKTSFLSALIA
                     AVPVSHCVRACEEVRELHVPLIHGSYYEARPPALDGGGEIALRDLVKVTLAMRPDLIV
                     VGEVRGAEAFELTRAANAGCGMVCTIHANSSTDALAALVNAALMAGENVPERVVRKIF
                     STSIDFLVHLDRDVEPSEGKIRRQTVEIRALVPSLHEDFSSEPLFVRERMGGPMVWTG
                     ALPPEPVARRLQDALPEGLTLTEVMKGAQSSW"
     gene            complement(1649..2746)
                     /locus_tag="AC161X_09380"
     CDS             complement(1649..2746)
                     /locus_tag="AC161X_09380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR4989806.1"
                     /translation="MSVGPELALAASARDWPDRLHRFLLDHGGGRVLARVMGPEQAIH
                     DQYDVLLIDDVCSFLTPRLVHRLREAGREIVGVYSPGDGPDAKRRLLECGITDVIETD
                     AEPEEFVAMSAAILTHRVVVTREGPSGSAAYRIGVTGSGAGTGVTEVAIALASELGSR
                     GSALLIDLNQKWPALAQRLDLALHPNLRTAVDLAHHSPSRLDEAVHECGSLAVVTGLA
                     NPGTGQEVPDHEMSGLMDDLAMRGYEFLVADLGPLADLSGLDEFSLLIVVGTASPVGL
                     TRLVQSIEKVPTGFRPPLLVVVNRAPAGGHARSEIRSELSRSIPGVPVVLLPGDPRIE
                     AAAWNGVIADRGGFHRAIKDMAGLLIDANRS"
     gene            complement(2743..3420)
                     /locus_tag="AC161X_09385"
     CDS             complement(2743..3420)
                     /locus_tag="AC161X_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0009297 - pilus assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaB family protein"
                     /protein_id="HIR4989807.1"
                     /translation="MATGPSVISREAGTSSPPAKPARRRPSLSHVLIAVTVILAFGLN
                     YLALKSRDAQVLVGVAAVPISEGSPLTADVVKFVPLAADFEAVGTLLLENELEDLDGR
                     IFARSIPIGGLVDESALIEPGAEDGHRAMSIPVAIEHAAGARIVVGDRVDVITVVDGV
                     ARFVATDLAVIAHADTETGGLSTMAYHLTVAVDDDQALALAEAIASGSVEIVRSTGAA
                     PMSESEG"
     gene            3474..4004
                     /locus_tag="AC161X_09390"
     CDS             3474..4004
                     /locus_tag="AC161X_09390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989808.1"
                     /translation="MNEQENHDPELESVLREAGREMNEEAAEDERLTELLRRRRLGMP
                     ELALEWVHRGQRVRAESGTQTFSGQVVFAGTDYVTIERTEDQVAVRLDRATWTLESEP
                     GAGREQSGDATSLKAHLAGLASSGEMVRLVVAEGRALIGSIAVVAADQVEIRQDHLTV
                     LVPNDLILAVITSHHH"
     gene            4123..5019
                     /locus_tag="AC161X_09395"
     CDS             4123..5019
                     /locus_tag="AC161X_09395"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021509.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oxygenase MpaB family protein"
                     /protein_id="HIR4989809.1"
                     /translation="MRERIVSSATSPFTHAAYPLEHTLDYPNDPGLLGPGSISWLVFA
                     DVATFIGGIRALILQAAHPEVVAGVGDHSRYREDPLGRLSRTSAYVTATTYGAMPEVD
                     AGVTQVRRIHRVVSGVSSRGLPYDAGDPGYAAWVHNALTDSFLVAHQTYGRLTLSPSD
                     QDAFVVEQTRVGALLDSDPMPITASELSRWIGLHPELAPSPEMREAVDFLVDPPLEPG
                     IKIGYKILLEAAVATLPRRLLSILGLTKTPGAVTAGRVAISGLRWALGDSPSWKLALL
                     RTGSPIPEGRFSRTIQREPHGR"
     gene            5009..5362
                     /locus_tag="AC161X_09400"
     CDS             5009..5362
                     /locus_tag="AC161X_09400"
                     /inference="COORDINATES: protein motif:HMM:NF020397.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HIR4989810.1"
                     /translation="MADNELTVDEFVNTKVLPQFRPVVEMLRKLMREHVPDAREEIRY
                     GIPNYTRNKGLAVISPTKKDITFAFSRGASFEDRYHLLQGVGHVSKHIKLRSVDDVAE
                     DVLIYYVDQALKFDS"
     gene            complement(5359..>5723)
                     /locus_tag="AC161X_09405"
     CDS             complement(5359..>5723)
                     /locus_tag="AC161X_09405"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HIR4989811.1"
                     /translation="PHYGIYDLLVRNATRYDWPFVSRYVLKARPEEAPELYRLGSPID
                     LVRLDAPPFLLVHGDFDSIVLVEESRHFADALESVGADVRLLEVHGAQHGFDAFASLR
                     TRSVDHAVAAFLIEVAGS"
BASE COUNT         1127 a         1856 c         1758 g          982 t
ORIGIN      
        1 ctcgatcgca gtgggcagcg atgcacccga ccgaaccgaa gagatgaggt ctcgcagacc
       61 gtccggccac gcctcctgga cctcggccaa tcgccgccct cgcttacggc cgaaataggc
      121 ccggggaagg gtggcgacta cgaacgcggg catcgccgag atgaccggca gagatgtgac
      181 cgcgtagacc aacaggtagg tcaccactcc ggctccgaga gacgccagcc agaactgggt
      241 cggagtgaca tcgagccccg cctgcgactg ccacggcgct tcgaccgggg ccaccttctg
      301 acggcggcga taggccgaat gaacggcgat tactacaagg gcagccgctg ctgcggtcgc
      361 tgccgccgca aggaaggtca ccatgatgac tgagcgccct tcatcacttc cgtcagggtc
      421 aatccctcag gcaaagcatc ctggagccgg cgggcaactg gctcgggcgg taaagcaccg
      481 gtccaaacca tcggtcctcc catccgttcc ctgacgaaca gcggctccga cgagaagtct
      541 tcgtgaagcg agggaaccag cgcgcgaatt tcaacggtct gacgacggat cttcccctca
      601 gatggttcga cgtcccgatc caggtggacg aggaagtcga tcgaagtcga gaagatcttt
      661 cgtaccacgc gctccggcac gttttcgcct gccatcaagg cagcgttgac caacgcggcc
      721 agcgcatcgg tggaggagtt ggcgtgaatc gtgcagacca tgccgcaacc tgcattggca
      781 gctctggtga gctcgaacgc ctccgctccc cggacctcac cgaccacgat cagatccggg
      841 cgcatcgcca gggtcacctt caccaggtcg cgaagtgcga tctcgccgcc accgtcgagc
      901 gccggaggcc ttgcctcgta gtaggagcca tggatcagag ggacatgcag ttcacgtacc
      961 tcctcgcagg cgcgcacaca atgggagacc ggaactgccg cgatcagagc ggacagaaac
     1021 gaagtcttcc cggcgccagg aggaccgctc acaatgaccg acgccgatgc ccgcatcgcg
     1081 gacgaaagaa atgaggcggc ggcgggggtg agcgaggcga ggcccacgag ggagtccatc
     1141 gtctctctac gcaaggcgta gcgtcggatc gtggccgaga ggcgatccgc cacaggtggg
     1201 atcaccgccg tcagacgtgc ggttccgtca agaatccttg cctgcgcgat tggattcgaa
     1261 gcgtcgagac gccgatcggt cgttgcgatc aggcgctcga tcagctgacg attctcctcc
     1321 tcgctggttg gggtctccag gccccgtagc catccgccgg cttccaggta ggtaacgctg
     1381 tcaccttcga tgaagatctc ctcgacgtcc tctcgctcga gcagatcgcc gaacggcccg
     1441 tgtgccgaga ccgatcgata cagcctccgg accgtttctt ctggattggc cagtgaacgg
     1501 ccctctccaa ggtgggccaa tctttggtag tcgtcgactg cctcctccag aagcttcatc
     1561 aagggccccg actcctcagc cggatcgatg cctgcgccgt cgattcgctc caaagcacgt
     1621 cttcttatct cctggtaggc gtcagtcgtc atgagcggtt cgcatcaata aggaggcccg
     1681 ccatgtcctt gatcgctcga tggaacccgc cccgatctgc gatcactcca ttccaggcgg
     1741 cagcctcgat tctcgggtca cccgggagaa gcacgacggg caccccggga atcgagcggc
     1801 tcaactctga acggatttcg cttcgagcgt ggccacccgc cggcgctcgg ttgactacca
     1861 cgaggagggg tggccgaaag ccggtgggca ccttctcgat gctttggacg agacgtgtga
     1921 gtcccaccgg actcgctgtt ccaaccacga tgagcaggct gaattcatcc aggccagaca
     1981 ggtcagcgag gggcccaagg tcggcgacca agaactcata cccccgcatc gccagatcgt
     2041 ccatcaaacc gctcatttcg tggtcaggca cctcctgacc ggtgcccggg ttggctagac
     2101 ccgtcactac ggcaagtgag ccgcactcgt gaaccgcctc gtcgagtcgg ctgggcgagt
     2161 ggtgggccag atcgacggcg gtcctcaggt tcggatgcag ggccaggtcc agacgctggg
     2221 ccagtgccgg ccatttctga ttcaggtcaa ttagaagagc ggatcctcga ctccccaact
     2281 cgctggctaa ggcaatcgcc acctcagtga cgccggtgcc ggcgcccgat ccggtcaccc
     2341 cgattcgata ggccgcagac ccggaagggc cttcccgagt tacgaccact cgatgggtga
     2401 ggatggcggc cgacatcgcg acgaactcct ctggctcagc gtctgtctcg atcacatcag
     2461 tgatcccaca ttcgagaaga cgccgcttgg catccggtcc gtcgcccggg ctatagacac
     2521 caacgatttc gcggccggcc tctcgcaggc ggtgcaccag ccttggagtg agaaacgagc
     2581 acacgtcgtc gatgaggagg acgtcatatt gatcatggat tgcctgctca gggcccatga
     2641 ctcgggccag gacgcggcct ccaccgtgat cgagcaggaa acgatgaagc cgatcaggcc
     2701 agtcacgggc ggaggcagcc agtgccaact caggccccac gctcacccct cagactctga
     2761 catcggcgcc gcccctgttg agcggacaat ctccacggag cctgacgcga tcgcttcggc
     2821 caacgccagg gcctggtcgt cgtcgacagc gaccgtgagg tgataggcca tggttgacag
     2881 accgcccgtc tcggtgtcag cgtgggcgat caccgccagg tcggtcgcta cgaaccgggc
     2941 aactccgtcg accaccgtga tcacatcgac ccgatcgccg accacgatcc gggctcctgc
     3001 tgcatgctct atggcgaccg gaatgctcat cgcccgatga ccatcctccg ccccaggctc
     3061 gattagcgca ctctcatcca cgagcccgcc gatcggaatt gaccgagcga atatccggcc
     3121 atccaggtcc tcgagctcgt tttcgaggag aagggtccca accgcctcga agtcggctgc
     3181 cagtggcacg aacttcacga catcggccgt cagcggagac ccttccgaaa tcgggacggc
     3241 agccactccc acgaggactt gtgcgtctcg gctcttgagg gccagatagt tgaggccgaa
     3301 agccaggatc accgtgaccg cgatcagaac gtgcgacagt gaaggtcgac gacgcgccgg
     3361 cttagccggt ggtgaagagg tgcccgcttc cctcgatatg acagagggac ccgttgccac
     3421 ggggcgacaa cctatcttcg ggatgacacc cgatgtcgag agcgcgaaaa gacatgaacg
     3481 agcaagagaa tcacgatccg gagctggagt cggtgctccg cgaagcgggt cgagaaatga
     3541 atgaggaggc ggctgaagac gagcgcctca ccgagctcct ccgacgtcgg cggctgggga
     3601 tgcccgagct cgcattggag tgggtccatc gaggacagag ggtgcgagcc gagtcgggga
     3661 cccaaacttt ctccggccag gtggtgttcg cgggcaccga ctacgtgacc atcgagagaa
     3721 ccgaggacca ggtcgcggtc cgactggacc gggccacctg gaccctcgaa tccgagcccg
     3781 gcgccggtcg cgagcagtcc ggggatgcca ccagcctcaa ggcccatctg gcgggactgg
     3841 cgtcgagcgg tgagatggtg cgccttgtgg ttgcagaagg tcgagcgttg atcggttcga
     3901 ttgctgtagt tgccgccgat caggtcgaga tccgacagga ccatctcacg gtgctggtgc
     3961 ccaacgacct gatccttgcg gtcatcacct ctcaccacca ctgacatctc gcggcccacg
     4021 atcacgcgac cgacagtacg ggcctctcag cttggagtta agggggttga taatgagccc
     4081 gtcttgcaaa ttccgacatc cctgagtggc cgcttggagg gcttgagaga gaggatcgtg
     4141 tcctcggcga cctcaccttt cacccacgcc gcctatcccc tcgagcacac cctggactac
     4201 ccgaacgatc ccggcttgtt aggcccggga tcaatctctt ggctggtgtt cgccgatgtg
     4261 gcgaccttca tcggagggat tcgggctctg attctccagg ccgcgcatcc cgaagtggtg
     4321 gcaggcgtcg gcgatcattc gagatatcgg gaagaccctc tgggccgctt gtcgagaacc
     4381 tcggcctacg tcacagccac cacctacggg gccatgcccg aagtcgacgc aggcgtgaca
     4441 caagtgcggc gaatccaccg tgtggtctcg ggagtctcga gccggggcct tccgtacgac
     4501 gcaggcgacc cgggctacgc ggcgtgggta cacaacgccc tcaccgattc attcctggtc
     4561 gcccaccaga cctatgggcg actcaccctg tccccctccg accaggacgc ctttgtggtc
     4621 gagcaaaccc gggtgggggc gcttctcgac tcggacccga tgccgatcac cgcgtctgag
     4681 ttgtcgagat ggatcggcct ccatcccgaa ctggctccat cgccggagat gcgggaggcg
     4741 gtcgactttc tcgtcgatcc gcccctggag cccggaatca agatcggcta caagatcctc
     4801 ttggaagctg cggtggccac ccttccccgt cgcctcctgt cgatcctcgg cctgaccaag
     4861 accccgggtg cggtgaccgc cggccgggtg gcaatctcgg gtctgcgttg ggctctgggc
     4921 gactcgccat catggaagtt ggctttactc cggaccggat cgccgatccc ggagggaagg
     4981 ttcagccgca cgatccagag ggagcctcat ggcagataat gagctgaccg tcgacgagtt
     5041 cgtcaacacc aaagtcctgc cgcaatttcg gccggtcgtc gagatgcttc gcaagttgat
     5101 gcgggaacac gtcccggatg cccgcgagga aatcagatac ggcattccca actacacaag
     5161 gaacaaaggc ctcgcggtga tcagccctac caagaaggac atcacattcg ccttctcccg
     5221 tggcgcctcc ttcgaggacc ggtaccacct cctccaaggc gttgggcatg tctccaagca
     5281 catcaagctc cgatcggtcg acgacgtcgc ggaggacgtc ctcatctact acgtcgacca
     5341 agcgctgaag ttcgattcct agctcccggc tacctcaatc aggaaagccg ctaccgcgtg
     5401 atcgaccgac cgggtgcgaa ggctggcgaa ggcgtcgaaa ccgtgttgag ctccgtggac
     5461 ttcgagcagc cgcacatccg cccctactga ttccaacgca tcagcgaaat ggcgggactc
     5521 ctcgacgagg acgatcgagt cgaagtctcc atggaccaag aggaagggcg gggcgtcgag
     5581 ccggaccaga tcgatcggcg agcccaggcg gtacaactcc ggggcttcct cgggtcgggc
     5641 cttgagcaca tagcgggaga cgaacggcca gtcgtagcga gtggcattcc tgaccaacaa
     5701 gtcgtagatc ccgtaatgag gga
//
LOCUS       DBNHXU010000385         4147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1149411, whole genome shotgun sequence.
ACCESSION   DBNHXU010000385 DBNHXU010000000
VERSION     DBNHXU010000385.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4147
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1149411"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /locus_tag="AC161X_09410"
     CDS             <1..297
                     /locus_tag="AC161X_09410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989812.1"
                     /translation="ARFAEIGPMTASTAEFFTDWTAHPVGKVTRALIAVVGVIVATLV
                     MLLATLGAQNAWILILVGVALAATSIRAAMEPTAIRLTTLAVTLMAIPIVSQVL"
     gene            611..1735
                     /locus_tag="AC161X_09415"
     CDS             611..1735
                     /locus_tag="AC161X_09415"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4989813.1"
                     /translation="MTSRFSHRGSPELEATLRRMVVIFRFLGLVWMAILVVITLAVDP
                     PPKLWIVYASVGLAVIWTLLTWYMSRAHPKAMSSIGWFALDSLAMLAIGAASVASGAE
                     ELFHGGLPLSLVFTGALVGGLPGSLVAALLLGVEQFLVHIIADLGAVRAAGSVIFFVV
                     GAIVGWTFDKLRDYDIARQEAQARLAEEQAVVLLHEQRAALADRLHDSVLQTLHAIRM
                     GADDPAQSRYLARRQERELRRNIEEWRSDFPNSFRASLLAVRDEIEDTHRVEIEAVIR
                     DDAQLNASLNAAVEAAREALANAAKHSGSPNIALYAEFSNGDAHIHVRDRGTGFTDEG
                     ARMRVHERLAKRVEAVGGVVEIDSGQESGTEIKITVEVKA"
     gene            1732..2382
                     /locus_tag="AC161X_09420"
     CDS             1732..2382
                     /locus_tag="AC161X_09420"
                     /inference="COORDINATES: protein motif:HMM:NF012301.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR4989814.1"
                     /translation="MTDRLRILLVDDHDVVRAGMRAILDHSFEIVGEADNVDSAIELT
                     RERNPQLVVLDVKLPGGGGASVIDAIRPQQPDIRFMALTVSTSREDVARLMEAGVDGY
                     ITKTTLGTDLPDLIHQTYEGARPVSPDVAGYLLDIDEDIVAESSISRLTPREREVVNL
                     IARGYTYRETASRMGIRVKTLENHMAHIFDKLSVASRHELTALAYEEGYLRPDDER"
     gene            complement(2384..3361)
                     /locus_tag="AC161X_09425"
     CDS             complement(2384..3361)
                     /locus_tag="AC161X_09425"
                     /EC_number="2.3.1.-"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:TIGR00747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP synthase III family protein"
                     /protein_id="HIR4989815.1"
                     /translation="MAAFLGFGGYVPERVMTNDDWAVLVDTTDEWITQRTGIKRRRVA
                     AEDETTLTLAANAAEVAIKDAGLIPADIGEIIIATDTPELYTPDTAALLQHRLGCDHI
                     PAYDLGGSGCAGFVQALDIARARISFSPRNILVVGVELITRLLDWSDRAVSVLFGDAA
                     GAVVLGPEGGRARLLEVVSGTDGSQAEILTLATGGTRNPFNEASLAAGDHKRLVMDGP
                     KVFTEAVKRMSASVDDLLARIDKTTDDVDLVIPHQANLRIIDAVGRRLGLSEEQVYIN
                     IQEYGNTGSATVPFALWEAVETGRIKEGDLVVLTAFGAGLHWASAALQF"
     gene            3447..3719
                     /locus_tag="AC161X_09430"
     CDS             3447..3719
                     /locus_tag="AC161X_09430"
                     /inference="COORDINATES: protein motif:HMM:NF014229.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UBP-type zinc finger domain-containing protein"
                     /protein_id="HIR4989816.1"
                     /translation="MGWTREQPPCEHLGLIQEVEPGGSGCGECLAIGDTWVHLRLCLS
                     CGNVGCCDQSKNTHARKHAAASEHPIVRSFEVGEEWRWCFPDSRFV"
     gene            3721..>4147
                     /locus_tag="AC161X_09435"
     CDS             3721..>4147
                     /locus_tag="AC161X_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017739802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HIR4989817.1"
                     /translation="MPKPVILTVDDDPEVLRAVQRDLRKHYSEEFRVVAANSGTEALE
                     TLKELSLRNEAVALLLVDQRMPDMSGVQFLAQTLERLPDAKKALLTAYADTEAAIAAI
                     NEVSLDHYIMKPWDPPEEKLYPILDDLLEDWWDHFTPRFE"
BASE COUNT          784 a         1333 c         1277 g          753 t
ORIGIN      
        1 gcccgatttg ccgaaatagg gcctatgacc gcatcaacag ccgagttctt caccgattgg
       61 actgcccatc cggtcggaaa ggtgaccagg gcactgatag ccgtagtcgg cgtgatcgtg
      121 gccaccctgg tcatgctcct ggcgactctc ggagcccaaa acgcctggat tctcatcttg
      181 gtcggtgtcg cgctcgccgc gacatcgatt cgtgctgcga tggagccgac cgctatcaga
      241 ctgaccacgc tcgccgtgac gctgatggcg atccccatcg tcagccaagt tctctaagcc
      301 cccgcgaagg gtctcaacga cccgacgctg acccacacgg ccaccggcta acggtggccg
      361 tgtcttgcgt ctgatggagg gtcgccctgg gccccgcatg ggggagatcc cccataggcg
      421 atctgagctg ccaccgcgat catcacgttc ggggacttca gggaaaccac gtcccttgaa
      481 gcgacaatgg ggaaccccat ggggggaccg cctcggcagt ccccccatgg ttcaacccca
      541 ccttgctccc atggtcatca acgcaatcaa aatggatccc agggacgccg aaggggtagc
      601 ttcggccacc atgacctcga gattcagtca tcgcgggagc ccggagctcg aggccacgct
      661 ccgccggatg gtggtcatct tccgattcct cggattggtg tggatggcga tcctggtggt
      721 gatcaccctg gccgtcgatc cgccaccgaa actctggatc gtctacgcat ccgtcggcct
      781 ggccgtgatc tggaccctgc tcacctggta catgtcgcga gcccacccca aggccatgag
      841 ctcgatcgga tggttcgccc tcgattcgct ggcgatgctc gccattggtg ctgcctcggt
      901 ggcgtcgggc gccgaggaac ttttccatgg cggccttccc ctctcattgg tattcaccgg
      961 ggcactggta ggaggtttgc caggctccct ggtagccgct ttgttactcg gcgtggagca
     1021 gttcctggtc cacatcattg ccgaccttgg agcggtcagg gcggccggga gcgtcatctt
     1081 cttcgtcgtg ggggcgatcg ttggatggac gttcgacaag ctgcgcgact acgacatcgc
     1141 aaggcaggaa gcccaggcac gtcttgccga ggaacaggct gtggtgttgc ttcacgaaca
     1201 gagagccgcc ctggccgacc gcctccatga ttccgtcctg cagacattgc acgcgatacg
     1261 aatgggagcc gacgaccccg cgcagtcccg ttatctcgcc agacgtcagg aacgcgagct
     1321 ccgccgcaac atcgaagagt ggcgctcaga ctttcccaac agcttccgcg cctctctcct
     1381 tgcggtgcgg gacgagatcg aagacaccca tcgtgtcgag atcgaggcgg tgatccgtga
     1441 cgatgctcag ctcaacgcca gtctcaacgc cgcggttgag gcggcacgcg aggccctggc
     1501 taacgcagcc aagcactccg gatcgcccaa catcgccctc tacgccgagt tcagcaacgg
     1561 cgacgcccac atccatgtcc gggaccgggg caccggattc accgacgagg gagcacggat
     1621 gcgggttcac gagcggctcg ccaaacgagt cgaagccgtc ggcggagtag tggagatcga
     1681 ctccgggcaa gagtcaggca ccgaaatcaa gatcaccgtt gaggtcaagg cgtgacggac
     1741 aggcttcgaa tccttctcgt cgacgatcat gatgtcgttc gggccgggat gcgggcaatc
     1801 ctcgatcact cctttgagat cgtgggagag gcggacaacg tcgatagtgc gatcgagttg
     1861 acccgggagc gaaatccgca gttggtggtg ctcgacgtga agctgccagg gggcgggggc
     1921 gcctcggtca tcgacgccat ccggcctcag caacccgata ttcgattcat ggcactgacc
     1981 gtctcgacct cgcgcgagga cgtggcccgg ctgatggagg ccggagtcga cggctacatc
     2041 accaagacca ccctggggac cgacctcccc gacctcatcc accagaccta tgagggagcc
     2101 cgaccggtct ctcctgatgt tgccggttac ctgctcgaca tcgacgagga catcgttgcc
     2161 gaatcgtcga tctcgcgact cactccgagg gagcgcgaag tggtcaacct gatcgccagg
     2221 ggttacacct accgggagac cgcttcacgg atggggatca gggtcaagac cctggagaac
     2281 cacatggctc acatcttcga caagctgagt gtcgccagcc gccacgagct caccgcactg
     2341 gcctacgaag agggctacct ccggccagat gatgagagat gacctaaaac tgcagcgcag
     2401 cggacgccca gtgcagcccg gcgccgaatg cagtcagcac caccaggtcg ccttccttga
     2461 tccgcccggt ctcgaccgcc tcccacagcg caaacggcac ggtcgccgag ccggtgttcc
     2521 cgtactcctg gatgttgata tagacctgtt cctcgctcag ccccaacctg cgcccgacgg
     2581 cgtcgatgat cctcagattg gcctgatggg ggatcaccag gtcgacatcg tcggtggtct
     2641 tgtcgatccg ggccagaaga tcatctaccg atgcgctcat ccgtttcacc gcctccgtga
     2701 acaccttcgg gccgtccatg accagccgct tgtggtcacc ggcagcgagg ctggcttcgt
     2761 tgaacggatt cctggtgccg ccagtggcga gggtgaggat ttcggcttgg gagccgtcgg
     2821 tccctgaaac cacctcgagc agcctggcac ggcctccctc gggaccgagt accacagccc
     2881 ctgcggcgtc gccaaagagg actgaaacgg cccggtcgga ccagtcgagg agcctggtga
     2941 tgagctcgac gccgaccacc aggatgttcc gcggggaaaa tgagatgcgg gcccgggcga
     3001 tgtccagcgc ttggacgaaa cccgcgcagc cgctcccacc gaggtcgtaa gccggtatgt
     3061 ggtcgcagcc gaggcgatgt tggagaaggg cggctgtgtc cggcgtgtag agctccggag
     3121 tgtccgtggc gatgatgatc tcgccaatgt cggcagggat cagcccggca tccttgatgg
     3181 cgacctcggc agcattggcg gccaaggtga gggtggtctc atcctcggcg gcaacccggc
     3241 gacgcttgat gccggtccgc tgggtgatcc attcgtcggt cgtgtcgacc aggacggccc
     3301 aatcgtcatt ggtcatgact cgttcgggca catatccccc gaaacccaag aacgctgcca
     3361 tcgcgattca ggttagggcc gttcgcagag cacccgacaa tggcctagat ttcgcagcag
     3421 gtttcggaca ggcagggaag aacgaaatgg gatggactcg cgaacaacca ccttgtgagc
     3481 acctgggcct gattcaggag gtcgaaccgg gcggctccgg ctgtggcgag tgcctcgcga
     3541 tcggggacac ctgggtccac ctgcgcctct gtctcagctg cgggaatgtc ggctgctgcg
     3601 atcagtcgaa gaacacacat gcccgcaagc acgccgctgc ctccgagcat ccaatcgttc
     3661 gctcgttcga ggtcggcgag gaatggcgtt ggtgcttccc cgactctcga ttcgtctgac
     3721 atgccaaagc ctgtgatctt gaccgtcgac gatgaccctg aggtgctgcg cgcggtccag
     3781 cgtgaccttc gaaagcacta cagcgaggag ttccgggtgg tcgcggccaa ctccggcacc
     3841 gaggccctgg aaaccctcaa ggagctgagc ctcagaaacg aagcggtcgc ccttctcttg
     3901 gttgaccaga ggatgcccga catgtcggga gtccaattcc tcgctcagac cttggagcgc
     3961 ctgcccgacg ccaagaaggc actgctcacg gcgtatgccg acaccgaggc tgccatcgcg
     4021 gccatcaacg aggtgtccct cgaccattac atcatgaagc cctgggatcc ccccgaggaa
     4081 aagctctatc cgatcctcga cgacctcctg gaggactggt gggatcactt caccccacgt
     4141 ttcgagg
//
LOCUS       DBNHXU010000386         4274 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_156977, whole genome shotgun sequence.
ACCESSION   DBNHXU010000386 DBNHXU010000000
VERSION     DBNHXU010000386.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4274)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4274)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4274
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_156977"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="AC161X_09440"
     CDS             complement(<1..732)
                     /locus_tag="AC161X_09440"
                     /inference="COORDINATES: protein motif:HMM:NF025757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtrH N-terminal domain-containing protein"
                     /protein_id="HIR4989818.1"
                     /translation="MMTQHKKLKQRIRARTDKTGESYTTARRQVVGVEEGTGTRLGVD
                     PDTSALSRILADSGVTGPTGPLNEGLLLGIGGGLGAGYILWEFSPETVRKSSHHSDGR
                     RRVVITGFRNRWQYPDRWADTVLDRLGVTFRREQTGSIARASQQLDEALASGKSVMAD
                     ISVADLPYWHMPVVEAGCWGYPVAVTGVEGGRYVVDDRSQGRLTVEAGALSIARNRIP
                     SYKNRIVMIEESQQLGVVAVTNAIEA"
     gene            complement(1072..1368)
                     /locus_tag="AC161X_09445"
     CDS             complement(1072..1368)
                     /locus_tag="AC161X_09445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003935456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SHOCT domain-containing protein"
                     /protein_id="HIR4989819.1"
                     /translation="MPRLMRTMARTAVVAGTATAVSGRVARRQNDKWQDQADAQAYQE
                     QQAAPPPQAYAAPAPAAPSTDDKLGQLKELGALKEAGVLTDAEFEAQKAKILAG"
     gene            complement(1379..1831)
                     /locus_tag="AC161X_09450"
     CDS             complement(1379..1831)
                     /locus_tag="AC161X_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020135274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6325 family protein"
                     /protein_id="HIR4989820.1"
                     /translation="MTDNDIEVGPVDYFVVEWPIGSPPTGEAFPYLVDLVDRGLIHIL
                     DLAFVQKDMEGNILAMELGDFDLDGNPDLAVFQGASSGLIDEEDKMEAGGIMEPGTAA
                     AILVYENSWAAPFATALRKTGARLVAYGRIPINDLIATLDALEAAEAS"
     gene            complement(1959..3554)
                     /locus_tag="AC161X_09455"
     CDS             complement(1959..3554)
                     /locus_tag="AC161X_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005256026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HIR4989821.1"
                     /translation="MSDPTPSSVGEGSFNRRLAILLAMAMFVLVVDTTLMNVSISAVV
                     GDLSTTVSAVQAAIALEALVSAAFILISSKIGDLIGRKRAYVLGLLGYAIGALTMTLT
                     DSLAPMIIFWAILGGLGASLLLPAMQSLIHGNFEGDAQKRVYALVGASAAIAAAFGPL
                     LGGFVTTYMSWRVGFLLEVVIIAIVLLGMGMVRDVPYTGDRRLDLVGAVLSIVGMGGV
                     VLGILVWQEGGEAVGILIAVGTIGLLLLARWLVRRKREGKVTLLDPDLLKSKMFRLGI
                     TGQMLQQIALGGSMIVIPIFLQMVLGYNAMQTGLTLAPLSLTMFAVSVRAGKRAGKRR
                     PAAVIRAGFGLLTLGIIVMIPIVPRTDTGWWLAIPLFIMGMGLGLLVSQLNNYALAPI
                     SGERISEAAGVNSAAGSFGLSFGLAFAGGIMLAALSLTFVNMSESSTVLPPQEQQQVA
                     TALEEDAEVMSNAQLEELLVGQPEAIQEEIIRINTEARPIALQFALLVPLVAGLIGFG
                     NSFRMLRLPDVTPSADIEGIIGG"
     gene            complement(3733..>4274)
                     /locus_tag="AC161X_09460"
     CDS             complement(3733..>4274)
                     /locus_tag="AC161X_09460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989822.1"
                     /translation="LLAGAVALTFIYIMGSVALGAGDPGDSAWIFAFLVMILFPASFG
                     VAVLRYRLFDIDRIVSRTVGYTLVAGTLAAVFALIVTVLTSLLPANSDNIVVAGSTLA
                     VAALFNPMRRRIQDSVDRRFNRSQYDARRVGDRFSNLVRDEVDMDRIVEGWVDVISET
                     MQPATIAIWLNGEAVATQT"
BASE COUNT          808 a         1375 c         1317 g          774 t
ORIGIN      
        1 cgcctcgatc gcgttggtga ccgcaacgac cccgagttgc tgtgactcct cgatcatcac
       61 gatccggttc ttgtacgacg ggatcctgtt tcgggcgatt gacagggcgc ccgcttcgac
      121 ggtgagcctg ccctggctgc ggtcatcgac cacatatcgg ccgccctcga ctccggtcac
      181 cgctacggga taaccccaac acccggcctc gaccactggc atgtgccaat agggcaggtc
      241 ggccaccgag atgtcagcca tgaccgactt cccggatgcc aaagcctcgt cgagctgctg
      301 gctcgctctg gcgatgctgc ctgtctgctc ccggcgaaat gtcactccaa ggcggtccag
      361 gacggtgtcc gcccagcgat cgggatactg ccaacggttg cgaaacccgg tgatcaccac
      421 acgacggcga ccgtcggagt ggtgggacga cttgcggacg gtctcgggcg agaactccca
      481 gaggatgtac ccggcgccga ggccgccccc gatcccaagg agcaggcctt cgttcagcgg
      541 acccgtgggc ccggtcacac ccgaatcggc aaggattcgg gaaagggcgg aggtgtcggg
      601 atcgacgccg agccttgtgc cggtgccctc ctccactccg accacctgcc tgcgggctgt
      661 cgtgtagctc tcaccggtct tgtcggtgcg ggcgcgaatg cgctgcttga gcttcttgtg
      721 ttgggtcatc atcgatctcc agtgaccacc gcctcagacc cacactccgt ctggcggccg
      781 tcacgagctc gacgacatcg acatcactcc cgatgacatc tggccacctt tccctgcgct
      841 tcgccggatg gtgtgggcac cggtctgcag gtgaggcgtg agagcacgcc ttgacagagg
      901 ctagctcagt attggaaggg ccatgaggtc aaccatccca tcgcgggggt gggatggcct
      961 caggtcttgg agatcagtca tccgtgcgca ggactttccg gccgagaaag agatccggaa
     1021 gcggatactc cgctcccgga tcggatcatt cgtcggaccg cgccacggtg gtcatccagc
     1081 cagaattttg gccttctgcg cctcgaattc ggcgtctgtg aggactccgg cttctttgag
     1141 ggcgcccaac tccttgagct gtcccagttt gtcgtcggtg ctgggggcgg cgggtgctgg
     1201 cgccgcgtag gcctgaggcg ggggagcagc ctgctgctcc tgataggcct gagcgtcggc
     1261 ctgatcctgc cacttgtcgt tctgccgacg tgccacccgg ccagaaactg ccgtcgccgt
     1321 acccgcgacc accgcggtgc gggccatcgt tctcatcaat ctcggcattt cgatcctcct
     1381 aactagcctc ggcggcttcg agcgcgtcga gggttgcgat caggtcgttg atagggatgc
     1441 ggccgtacgc caccagcctg gcgcccgtct tgcggagtgc ggtggcgaac ggggcggccc
     1501 aagagttctc gtagaccagg atcgcggctg cggtgcccgg ctccatgatg cctccggcct
     1561 ccatcttgtc ctcttcgtcg atcagacccg aggacgctcc ctggaaaacc gcgagatccg
     1621 gattgccgtc gaggtcgaag tcaccgagct ccatcgcaag gatgttgcct tccatgtctt
     1681 tctggacgaa cgccaggtcc aggatgtgga tcaggcctct atcgacgagg tcgaccaggt
     1741 aggggaaggc ctctccggtg ggaggagagc cgatcggcca ctccactacg aagtagtcga
     1801 cgggtccgac ttcgatgtcg ttgtcagtca catgtgtcct ttcgtcttag acgcgggcct
     1861 cagccctcct ggggatctta cgcgggcgga acctcgtgtg atccggcccg ccggcgcttc
     1921 caccagaagt cgacgatcgc gctgagacgg actgaggctc agcctccgat gatcccctcg
     1981 atatcagccg atggcgtgac gtccggaaga cgcagcatcc ggaacgagtt cccgaaccca
     2041 atcaacccgg ctaccagagg caccagcaac gcgaactgca gggcgatcgg tcgagcctcg
     2101 gtgttaatcc gaatgatctc ttcttggatc gcctcgggct gccccacgag aagctcctcg
     2161 agttgggcgt tgctcatcac ttcggcgtct tcttcgagcg ccgttgccac ctgctgctgc
     2221 tcctgcggag gaagaacagt gctgctctcg gacatgttca caaaggtcaa ggagagcgct
     2281 gcgagcatga tcccaccggc gaaagcgagg ccaaaggaga gcccgaacga tccggccgcc
     2341 gagttgaccc ccgctgcttc actgattcgc tccccggata tcggggccag cgcgtagttg
     2401 ttcaattggg agacgaggag gcctagcccc atgcccatga tgaaaagcgg gatggccagc
     2461 caccatccgg tgtcggtccg gggcacgatt gggatcatca cgatgatccc aagggtgagc
     2521 aagccgaatc cggccctgat cacggccgcc ggccgccgtt tgcccgccct ctttccggcc
     2581 cgaaccgaca ccgcgaacat cgtcaaggag aggggagcga gggtgagccc ggtttgcatc
     2641 gcgttgtagc cgagcaccat ctgcaggaag atcggtatca cgatcatgga accgccaagg
     2701 gcgatctgtt gcagcatttg accggtgatt ccaagccgaa acatcttcga tttaaggagg
     2761 tcggggtcga gcagcgtcac cttcccttcc cgcttgcgcc gcaccagcca tcgggccagg
     2821 agcaacaggc cgattgtccc gactgcaatg aggatcccga cggcttctcc gccctcttgc
     2881 cagacgagga tccccaggac cacacctccc atgcccacga ttgagaggac ggcaccgacc
     2941 aggtcgagtc gtcggtctcc ggtgtaggga acatcacgca ccatccccat cccaagcaag
     3001 acgatggcga tgatgaccac ctcgagcagg aaccccaccc gccaggacat ataggtagtg
     3061 acgaaacctc ccaggagagg cccgaaggcg gcggcgatgg ctgccgaggc cccgacgagg
     3121 gcgtagaccc gcttctgagc atcgccttcg aaattgccgt ggatcagcga ctgcatcgcc
     3181 ggaagcaaca gggatgctcc cagccctccg aggatcgccc agaagatgat catgggtgcc
     3241 aggctgtcgg tgagggtcat cgtgagcgcc ccgatcgcgt agcccagcaa acccaggacg
     3301 taggcccgct ttcggccgat caggtcaccg atcttgctgc tgatgaggat gaaggcggcc
     3361 gataccagcg cctccaatgc gatggccgct tgcaccgcgc tgaccgtggt actgaggtcg
     3421 ccgaccaccg ccgagatcga gacgttcatc aacgtcgtgt cgaccacgag gacgaacatg
     3481 gccatcgcca gcaggatggc cagccgccgg ttaaagctgc cttccccaac ggaggatggg
     3541 gttggatccg acacatcgct cctttgcgtg cgcacgccct accgaacgga atcggaggag
     3601 atcgagtgaa tctaaccctt cgcccgatct cgaagcgggt gatcgttgca ggtccggctc
     3661 gacggccggc accatccgag gaagggagat cgttcctcat tgcagacgtt cctcagattc
     3721 cgtcaatggt ttctaagtct gtgtcgcgac agcttctccg ttcagccaga tcgcaatcgt
     3781 ggccggctgc atggtctccg agatcacatc aacccagccc tccacgatgc ggtccatatc
     3841 cacctcgtcc ctgaccaggt tgctaaaacg gtcgccgacc cgtcgtgcgt catactggga
     3901 tcggttgaac ctccgatcca ccgagtcctg gatccgtcga cgcatcgggt tgaagagtgc
     3961 tgcgacggcc agagtcgacc cggcaaccac gatgttgtca ctgttggcag gcaggagtga
     4021 agtcaacacg gtgacgatga gggcgaatac ggccgccaac gtcccggcga ccagtgtgta
     4081 cccaacggtc cgggacacga tccggtcgat gtcgaagagt cgatagcgaa gcactgccac
     4141 cccgaatgag gccgggaaca ggatcatcac gagaaacgcg aagatccagg ccgagtcccc
     4201 tgggtcgccg gcgcccaggg cgaccgatcc catgatgtag atgaaggtga gggctaccgc
     4261 gcccgccaga aggg
//
LOCUS       DBNHXU010000387         2714 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_554731, whole genome shotgun sequence.
ACCESSION   DBNHXU010000387 DBNHXU010000000
VERSION     DBNHXU010000387.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2714)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2714)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2714
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_554731"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..487)
                     /locus_tag="AC161X_09465"
     CDS             complement(182..487)
                     /locus_tag="AC161X_09465"
                     /inference="COORDINATES: protein motif:HMM:NF017103.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DciA family protein"
                     /protein_id="HIR4989823.1"
                     /translation="MNGGDLSSFESSLGDVFRKMGLPDPVLMSQLIDEWDDLAGTPWV
                     GRSRPVVIKGRTLVVEASAPSMVAFLRYGATQLVETLSERFGEGVVDQVEVIPPSRN"
     gene            complement(484..1578)
                     /gene="recF"
                     /locus_tag="AC161X_09470"
     CDS             complement(484..1578)
                     /gene="recF"
                     /locus_tag="AC161X_09470"
                     /inference="COORDINATES: protein motif:HMM:NF014516.7"
                     /note="All proteins in this family for which functions are
                     known are DNA-binding proteins that assist the
                     filamentation of RecA onto DNA for the initiation of
                     recombination or recombinational repair; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HIR4989824.1"
                     /translation="MQVDWLSLSDFRSYESLQWSPDPGVNLLLGPNGAGKTNLLEAIG
                     FLATLRSFRSVDEGSLIRDGAAGAVVRAGISSDQRTRLVEIELNRTGPRRAQVDKTRL
                     RRAADLLGVVRVIAFLPEDLDLVKRGPAYRRDLVDEIALQLWPAAHLDQAEFDRALRQ
                     RNAFLRAGERDEATLSVWDSRFAQSGGRVMARRAAVLETLAPWLEESYREISQRTDLA
                     TLGYRCSWTTGEISTAASGEFATAIATALEASRKADFDRRLTTTGPHRDEPVFLIDGV
                     DTRTHGSQGEQRTMALAVKLAAHRAVADAVGEQPVLLLDDVFSELDPVRAKALAGAIP
                     QSAQTLITSARPEDVPVPGSVWRVEDGIRR"
     gene            complement(1579..2661)
                     /gene="dnaN"
                     /locus_tag="AC161X_09475"
     CDS             complement(1579..2661)
                     /gene="dnaN"
                     /locus_tag="AC161X_09475"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HIR4989825.1"
                     /translation="MRIRAQRDDLADVLARANRAVGTRTALPVLQGLLCEVSGTSLRV
                     TGTDLDITVRTSAEVEVLEEGRAVIPGRLLSEAVRKMPAGAVTIGSSDNDIEIQGNGP
                     RFTLRPLALDDFPLHEEIVSDGVEVDGEELAEAINQVTIAASGDGARPILTGVLFEGS
                     ETGLRMVATDSYRLARRDLTGVGLQGTALVPARGLRELPRTIGSTKVTAQLLDREAVF
                     TSDKGQLRLRLIDGSFPKYQSLLPDVYPNQVVVEKETLLDALGRVSLVAEDHIPVRLK
                     LMEGGIEVTVTRQDVGAEAEHLDGVYTGTDEEVSIAFNPRYLQDGVSAIPGDSVRIRV
                     IDAFKPSVIDHGADDEFLYLLMPVRV"
BASE COUNT          497 a          892 c          823 g          502 t
ORIGIN      
        1 acggcttcga gaccgtcgag aacggtgatg tctttggctt cgtagctgtc gcgggtggcc
       61 gtcacgaccc tccttatggg acctggatgg aaggtggagt ccgcctactc gcggatactt
      121 tcacgtatgt cattttaccg atatttactg acaaaaacca atccatccgt tgcgggagcg
      181 atcagtttcg cgacggcggg atcacctcaa cctgatcgac gacgccttcc ccaaaacgct
      241 ccgacaacgt ctcgaccaat tgggttgccc cgtacctgag aaaagccacc atcgagggcg
      301 ccgaagcctc gacaaccagg gtccggccct tgatcaccac cgggcgcgac ctgcccaccc
      361 acggggtccc ggccaaatcg tcccactcat cgatcagctg ggacatgagc accgggtcgg
      421 gcagccccat cttgcgaaag acatcgccaa gagaggattc gaacgacgag aggtccccgc
      481 cgttcatcgt cgaatcccgt cttcgaccct ccacaccgac cccgggaccg ggacgtcctc
      541 gggtcgggcc gaggtgatca aggtctgggc actctggggg atggccccgg cgagcgcctt
      601 ggcgcggacc gggtcgagct cggagaagac gtcatcgaga agcagcaccg gctgctcgcc
      661 gactgcatcg gccaccgccc ggtgcgcggc cagcttcacg gcgagggcca tcgttcgctg
      721 ctcaccctga ctcccgtggg tgcgggtgtc gaccccgtcg atcaagaaca cgggctcatc
      781 ccgatgtggg ccggtcgtag tcagccggcg atcgaaatct gctttgcgcg atgcctccaa
      841 ggccgttgca attgcggtgg cgaactcccc cgaggcagca gtggagatct cgccggttgt
      901 ccaggagcat cgatagccca gggttgcgag atctgtgcgc tgggagatct cccgataact
      961 ctcctcgagc caaggagcca gagtctccag caccgcggct cgccgcgcca tcacccttcc
     1021 tcccgattgg gcgaacctgg aatcccagac cgagagggtc gcctcatccc tctcgcccgc
     1081 tctgaggaac gcattgcgct ggcgcagggc acggtcgaac tcggcctggt cgagatgtgc
     1141 ggcgggccac agctggaggg caatctcgtc gacgaggtcc ctcctgtacg ccggtccgcg
     1201 tttgacgagg tccagatcct cgggaaggaa cgcgatgacc cgaaccaccc ccaacaagtc
     1261 cgccgctcgt cgcagcctgg tcttgtccac ctgggcgcgc cggggcccgg tccgattcag
     1321 ttcgatctcc accaatcgcg ttcgttgatc gctcgagatg ccggctcgga caactgctcc
     1381 cgctgcgccg tccctgatga gagagccctc gtcgaccgag cggaacgacc tgagagtcgc
     1441 cagaaaccca atggcttcca gcagatttgt cttaccagcc ccgttggggc cgagcaggag
     1501 attcacgccc gggtcggggc tccattggag agactcgtat gaccggaagt cagagagcga
     1561 taaccagtcg acctgcattc acactcggac gggcatcagc aggtagagga actcatcgtc
     1621 ggctccgtgg tcgatcacgc tcggcttgaa agcgtcgatc acccggattc gaaccgaatc
     1681 accggggatg gcggacacgc cgtcttggag gtatcggggg ttgaaggcga tggacacttc
     1741 ctcgtcggtc ccggtgtaga caccgtcgag gtgctcggcc tcggcgccga cgtcctgacg
     1801 ggtcaccgta acttcgatgc caccttccat cagtttgagg cgaaccggga tgtggtcctc
     1861 ggcaaccagg ctcacccgcc ccagggcatc aagcagggtc tctttctcga cgaccacctg
     1921 gttgggatag acgtcgggca gcagtgactg gtatttcgga aagctgccgt cgatcagccg
     1981 aagccggagt tgccccttgt cggaggtgaa taccgcttca cgatcgagca gttgcgccgt
     2041 cacttttgtc gagccgatgg tcctgggcag ttcccgcaat ccacgggccg gaaccagagc
     2101 cgtgccctgc agcccgaccc cggtgagatc tcttcgcgca agtcggtacg aatcggtggc
     2161 gaccatccgt aggccggtct ccgagccctc gaacaacacg ccggtgagga tgggacgggc
     2221 tccgtctccg gaggccgcga tcgtcacctg gttgatggct tcggccagct cctccccatc
     2281 gacttccacc ccgtccgaga cgatctcctc gtgaagtgga aagtcgtcga gggccagcgg
     2341 tcgcagagtg aaacgggggc cattgccctg gatctcgatg tcattgtcgg agctcccgat
     2401 ggtcaccgct ccagccggca tcttccgcac cgcctcggac agcaggcgcc ccgggatcac
     2461 cgctcgtccc tcctcgagaa cctcgacttc cgccgaggtc cgcacggtga tgtcaaggtc
     2521 ggtgccagta actcgcagac tcgtccctga aacttcgcag agaagtccct gaagcaccgg
     2581 caacgcggtc cgagtcccaa cggcgcggtt cgccctggcg aggacatcgg ccaggtcgtc
     2641 tcgctgagct cggattctca caggtggttg tcccttcttc tttttctact gagtgtcatt
     2701 taaagagtat tagt
//
LOCUS       DBNHXU010000388         1589 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2341144, whole genome shotgun sequence.
ACCESSION   DBNHXU010000388 DBNHXU010000000
VERSION     DBNHXU010000388.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1589)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1589)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1589
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2341144"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1028
                     /locus_tag="AC161X_09480"
     CDS             <1..1028
                     /locus_tag="AC161X_09480"
                     /inference="COORDINATES: protein motif:HMM:NF012415.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030115 - S-layer [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CAP and S-layer homology domain-containing
                     protein"
                     /protein_id="HIR4989826.1"
                     /translation="SGVKGRRPIVVALIIASLLVPQLALPAFAQTEGAYEVCLLDAIN
                     DSRAAAGAGALTMASDLVPPVREYSEWMRHHSFQHMSGSARNAILPDGTFTWGENIAM
                     WGDPSAHCDRVHDMLMDSPGHRANILNPNFRYVALGAHIDSSGEWVTELFFNANGYTP
                     GINGHFWDDDTSIFESAIEKFAAAGITQGCNPPVNDRFCPDDYVTRGMMAAFLVRGLG
                     LTDPGNVDFIDDNGSTFESAIEKLAAAGITQGCNPPQNNRFCPNQYVTRGMMAAFLVR
                     GLELTDQGNVDFIDDNGSLFEDAIEKLAAAGITQGCNPPQNNRFCPNDVVTRGMMAAF
                     LVRGLDL"
     gene            1054..1452
                     /locus_tag="AC161X_09485"
     CDS             1054..1452
                     /locus_tag="AC161X_09485"
                     /inference="COORDINATES: protein motif:HMM:NF013098.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4989827.1"
                     /translation="MSTPFESLDFLYIPTQDVAGDAAYFTDVLGGTLVFAIEEWRTRV
                     AMVEMTVGPPHLLFTDHVDGERPILIYRVEDLEATLERLTADGWERDSVFEIPQGPCC
                     SFRTPGGHRVAVYVLARPEVADHFKGRIDF"
     gene            complement(1459..>1589)
                     /locus_tag="AC161X_09490"
     CDS             complement(1459..>1589)
                     /locus_tag="AC161X_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007903509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HIR4989828.1"
                     /translation="PEERPLIYICGPTSFVETAAAALVDLGHDPGLVRTERFGPSG"
BASE COUNT          302 a          537 c          467 g          283 t
ORIGIN      
        1 aaagcggagt caagggtcgg cggccgatcg tggtcgccct catcatcgcg tccttgctgg
       61 tgcctcagct ggcgctcccg gcgttcgccc agaccgaggg tgcctatgag gtctgtctcc
      121 tcgacgctat caatgactcc cgagccgccg ctggggccgg cgccctgacg atggcctctg
      181 acctcgttcc ccctgtgcgg gagtactcag agtggatgcg ccaccacagc ttccagcaca
      241 tgtccgggtc ggcccgcaac gcaatactcc ccgacggcac cttcacctgg ggcgagaaca
      301 tcgccatgtg gggggatccg tccgcccact gtgatcgggt ccatgacatg ttgatggact
      361 ccccagggca ccgggccaac atcctcaatc cgaattttcg ctacgtggcc ctgggagcac
      421 acatcgactc ctctggcgag tgggtgaccg agttgttctt caacgccaac ggctacaccc
      481 cggggatcaa cgggcatttc tgggacgacg acacctccat cttcgagagc gcaatcgaga
      541 agttcgccgc cgccggcatc acccaagggt gcaacccacc ggtaaacgac cgcttctgcc
      601 ctgacgacta cgtcacgcgg ggaatgatgg ccgccttcct ggtccgcggc ctcggtctca
      661 ccgatcccgg caacgtcgac ttcatcgacg acaacggatc gacctttgaa agcgctatcg
      721 agaaactggc agccgccggc atcacccagg ggtgcaaccc accgcagaac aaccggttct
      781 gccccaatca atacgtgact cgggggatga tggccgcctt cctggttcga ggcctcgaac
      841 taaccgacca aggcaacgtc gacttcatcg acgacaacgg atcccttttc gaagacgcca
      901 tcgagaaact ggcagccgcc ggcatcaccc aaggatgcaa cccaccgcag aacaaccggt
      961 tctgccccaa cgacgttgtc acccggggca tgatggccgc cttcctcgtc cgcggcctcg
     1021 atctctagcg ccgtataccg ttcacggcga gccgtgagca ctccgttcga atccctcgac
     1081 ttcctgtaca tcccgaccca ggacgtggcg ggggacgccg cctatttcac cgatgtgctg
     1141 ggtgggacgc tggtgttcgc catcgaagag tggagaacca gggtggcaat ggtcgagatg
     1201 acagtgggtc ctccccacct cctcttcaca gatcacgtgg atggagaacg cccgatcctg
     1261 atctaccggg tcgaggacct cgaggccacc ctcgaacggc tcacggccga tggctgggag
     1321 agggactccg tctttgagat cccacagggc ccttgctgct ccttccgcac gccgggcggg
     1381 catcgagttg cggtctatgt tcttgcccgg ccagaggtag ccgatcactt caaggggcgg
     1441 atcgacttct gagcggaact agccggacgg gccgaatcgc tcggtcctga ccagacccgg
     1501 atcgtgcccc aggtcgacga gggcagccgc ggcggtctcg acgaaagagg taggcccgca
     1561 tatatagatc aacggtcgct cctcgggcg
//
LOCUS       DBNHXU010000389         4810 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_360433, whole genome shotgun sequence.
ACCESSION   DBNHXU010000389 DBNHXU010000000
VERSION     DBNHXU010000389.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4810)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_360433"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1148
                     /locus_tag="AC161X_09495"
     CDS             <1..1148
                     /locus_tag="AC161X_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020144715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family lipoprotein"
                     /protein_id="HIR4989829.1"
                     /translation="FMRVFAALAALALVLAACGDSGSGDTTTTAGGSSTTAGGDTTTT
                     GADAPTTTAAALIGEGRTIGMAFDVGGRGDLSFNDLAALAWDDGMAMYGYTGEELAPD
                     AGGENREENLRLLAESGQELIIANGFAFAQNVWRVGTEFPDTMFAITDDCPQDDTFTV
                     VEMPNVRCMLFSEEQGSFLMGVAAAMKSETGTVGFIGGVQVPLILKFQAGFEAGALAA
                     NPDITILPAVYLTQVPDFTGFNDPVKGKEAALALYGQGADVIFHAAGGSGLGLFQAAD
                     ELSTDESFLWAIGVDSDQYNTVSDPALQEHILTSMLKRVDVAVGKSITDFLEGSFTAG
                     AVRLGLAEEGVGYSKTGGFVDDIAADLDGYAAQIIDGTIVVPTEPAA"
     gene            1221..2747
                     /locus_tag="AC161X_09500"
     CDS             1221..2747
                     /locus_tag="AC161X_09500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013154251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989830.1"
                     /translation="MAQAPAIELRGVTKRYPGVVANKDVELTVLPSEVHAIVGENGAG
                     KSTLMKIIYGMVRPDEGTIAINGEPTAFKSPKEAIDAGIGMVHQHFMLADNLTVLENV
                     VLGSEPRSGLEIDFDFAANRIKELGDSYGLRLDPTQRVEALSVGERQRVEIIKVLYRG
                     ASILILDEPTAVLVPQEVDELFDSIRELTSEGQTVLFISHKLDEVLSIADRITVMRGG
                     MTVGTTTPAEVDARELAEMMIGSELPSPQTRESTVQDEVLLEVSDLGLLSADGRTVLD
                     GVTFKVRKGEIVGVAGVEGNGQGSLVDSIIGLASDATGEVTLAGESVSHLPARERRGA
                     GIGLIPEDRHRQGLLLSSPLWENAALGHQYQEPYANGIWIDKKGSRKQTEEIIRRFAV
                     KTPNVDVPATALSGGNQQKLVVGREIMADPVVLIAAQPTRGIDIGAQAAVWEELKAAR
                     AAGMGILLISADLEELIGLSDTIHVMFEGKFVAKLDPNQVTPEVLGSYMTGAGQEKAS
                     "
     gene            2744..3859
                     /locus_tag="AC161X_09505"
     CDS             2744..3859
                     /locus_tag="AC161X_09505"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989831.1"
                     /translation="MKRGRLLTAVVAPLLALIIAFGIAAIALLLVGIDPIQAYKEMIT
                     FGTTTASLIITVNDAIPLFVSALAVAVGFKMGLFNIGVEGAYLLAALVGAYVGALYSI
                     TPVLHVLSILLVCVAVGAVWALIPALLKVKRGVHEVISTIMLNFIAFSLSAWLFLNVF
                     KAESAGLTIATDLIPETGWIPSLNPVLGWFGIEPRAGSELHGFLIIAIFLGIGYHYLV
                     NKSRFGYDLRASGINPSAANASGVDPKAMVIKTMLISGGLAGLVGVSQLLGFYHQYPQ
                     DFPAGYGFSGIAVALLGRNNPVGMAFGALLFGFMNRSSLILDLKDVPKEIVTIIQGIV
                     VLAVVIVYEVISRYVQRRTVAAAARATEADEAAQVAA"
     gene            3856..>4810
                     /locus_tag="AC161X_09510"
     CDS             3856..>4810
                     /locus_tag="AC161X_09510"
                     /inference="COORDINATES: protein motif:HMM:NF014684.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989832.1"
                     /translation="MTTAEVVTRRTRLPARSRRIVIIALAGILVMTIAELITGTEDLT
                     SRSTASAALRLSIPILLAGLGGLYSERSGVVNIGLEGMMIAGTWFAAWGSWMFGPWEG
                     VALGIVGGAMFGLLHALATVTFNVDHIVSGVAINILGLGAMRFLSSVVYTEETGGSVI
                     QSPPIQGIENVSFPFLAGGQLFGWQSPDALGWLEEKSWFFVSDIAGIMRGFFGDVNWL
                     TLIGLAIVPLSAWLLWRTAWGLRLRSCGEDPWAAESLGVPVLRMKYYGVVISGMLAGL
                     AGAFLVLVQAGIYREGMSGGRGFIGLGAMIFGNWIPVGVLGG"
BASE COUNT          837 a         1413 c         1544 g         1016 t
ORIGIN      
        1 gctttatgcg ggtgtttgcc gcactggcag cattggcact ggtcctggcg gcctgtggcg
       61 atagcggtag cggtgacacc acaaccacgg cgggtgggtc gtccaccacc gccggcggag
      121 atacgactac tacaggtgct gacgcgccga cgacgactgc agcagccctc atcggagagg
      181 gtcggaccat cggaatggct ttcgacgtag gtggccgtgg cgacctgtca ttcaacgacc
      241 tcgcggcttt ggcgtgggac gacggaatgg cgatgtacgg ctacaccggt gaggagctcg
      301 ctcctgacgc cggtggtgag aaccgtgagg agaacctccg gctgctcgcc gagtcgggcc
      361 aggaactgat cattgcgaac ggcttcgctt ttgctcaaaa cgtctggcgc gtcgggaccg
      421 agttccccga cacgatgttt gccatcaccg acgactgccc ccaggacgac actttcaccg
      481 tggtcgagat gcccaacgtc cgatgcatgc tcttctctga ggagcagggt tcgttcctga
      541 tgggtgtggc cgccgcgatg aagagcgaga ccgggaccgt cgggttcatc ggtggcgttc
      601 aggtgccttt gatcctgaag ttccaggctg gtttcgaggc tggcgcactt gcagccaacc
      661 ctgacatcac catcctgcct gcggtgtatc tcacccaggt tccggacttc accggattca
      721 acgacccggt aaagggcaag gaagctgctc tcgctctgta cggacagggt gcggacgtga
      781 tcttccacgc tgccggtggt tccggtttgg gtctgttcca ggcggccgac gagttgtcga
      841 ccgacgagag cttcctctgg gccatcgggg ttgactccga ccaatacaac acggtgagcg
      901 atcccgctct tcaggagcac atcctgacct cgatgttgaa gcgggtcgac gttgcggtgg
      961 gcaagtcaat caccgacttc ctcgagggga gcttcaccgc aggtgcggtt cggctcggtc
     1021 tcgccgaaga aggcgtcggt tactccaaga cgggtggatt cgtcgacgac atcgcagctg
     1081 atctggacgg ttatgccgct cagatcatcg acgggacgat tgttgttccc acggagccgg
     1141 cggcctgacc taaggcactc ctgctaattt gcgccgggcc cagggttcct gggcccggcg
     1201 ttctttattg ggacgggaaa ttggcccagg cacctgcaat tgagctcaga ggcgtaacca
     1261 agcgttaccc cggtgtcgtc gccaacaaag acgtcgagct caccgtgctg ccctccgagg
     1321 tgcacgccat tgtcggtgag aacggggccg gcaagtcgac cctgatgaag atcatctacg
     1381 ggatggttcg ccccgacgag ggcaccattg cgatcaacgg cgaacccact gccttcaagt
     1441 cgccaaaaga ggcgatcgac gccgggatcg ggatggtcca ccagcatttc atgttggcgg
     1501 acaatctcac cgttctcgag aatgtggtgt tgggctcaga gccgcggtcc ggcctggaga
     1561 tcgactttga ctttgcagcc aatcgcataa aggagctcgg cgactcgtac ggattgcgtc
     1621 tggacccgac ccagcgagtc gaagccctgt cggtggggga gagacagcgc gtcgagatca
     1681 tcaaggttct ttaccggggg gcaagcatcc tcattctcga cgagccgact gctgtgctcg
     1741 tgccccaaga agtggacgag ctcttcgact cgatacgcga gctcaccagc gagggacaaa
     1801 cggttctttt catctcgcac aagctcgacg aggtgctctc gatcgccgat cgcatcaccg
     1861 tcatgcgtgg cgggatgact gttggaacca cgactcctgc tgaggtagat gccagggagt
     1921 tggcggagat gatgatcggc tcggagctcc catcaccgca aaccagagag tcgacagtgc
     1981 aagacgaggt gttgctcgag gtcagcgacc tcgggctcct gtccgcagac ggtcgcaccg
     2041 tgctggacgg agtgaccttc aaggttcgga agggggagat cgtcggtgtt gccggggttg
     2101 agggcaacgg ccagggatca cttgtcgact cgatcattgg cctagcttct gacgcgacgg
     2161 gcgaagtgac actcgccggt gagtcggtgt cacatcttcc cgcccgggag cgccgcggtg
     2221 ccgggatcgg cctcatccct gaggatcggc atcggcaagg gctgctcctg tcctccccac
     2281 tctgggagaa cgccgcgctt ggccatcaat accaggagcc ttacgccaac gggatctgga
     2341 tcgacaagaa ggggtcgcgc aagcagaccg aggagatcat tcgcagattc gcggtgaaga
     2401 ccccgaacgt cgacgtgccg gccaccgcct tgtccggggg caaccagcag aagttggtcg
     2461 tggggcgcga gatcatggcc gacccggtgg tcttgatcgc agcccaaccc acccgcggca
     2521 tcgacatcgg cgcccaggcg gcggtctggg aggagttgaa ggctgctcgc gctgccggca
     2581 tgggaatcct gctgatctcc gccgatctcg aggagttgat cgggctctcc gacaccatcc
     2641 acgtcatgtt cgaaggcaaa ttcgtggcga agttggatcc aaatcaggtt acgcccgagg
     2701 ttctcggctc gtacatgacc ggtgcgggac aggagaaggc ttcgtgaaga gggggagact
     2761 gctgaccgca gtggtggcgc cgctcctcgc gttgatcatc gctttcggga tcgcggccat
     2821 cgctcttctc ctggtgggca tcgatccgat tcaggcctac aaggagatga tcacctttgg
     2881 cacgacgact gcctcgttga tcatcaccgt caacgatgcc atcccactct tcgtatccgc
     2941 cctggccgtt gccgttggct tcaagatggg cctgttcaat atcggagtcg agggggcgta
     3001 cctgcttgcc gctctggtcg gggcctatgt cggcgccctg tactccatca cacccgtgct
     3061 gcacgtctta tcgattctcc tggtctgtgt ggcggtcggg gcggtttggg cactgatccc
     3121 tgctctattg aaagtgaagc gaggtgtcca tgaggtgatc tccacgatca tgctcaactt
     3181 cattgccttc tcgttgagtg cgtggctgtt cctcaatgtt ttcaaggccg agagtgctgg
     3241 gttgaccatc gcaacggatt tgatcccgga aaccggatgg attccgtcgc tgaacccggt
     3301 gttgggatgg tttgggatcg agccccgagc cggttcagag ctccatggct ttctgatcat
     3361 cgccattttc ctcggtatcg gctatcacta cctcgtcaac aagagccggt tcggatacga
     3421 cctccgcgcc agcggcatca acccgtcggc cgccaacgcc agcggggtcg acccgaaggc
     3481 gatggtgatc aagacgatgc tcatctcggg tgggctcgcg ggtctcgttg gagtatccca
     3541 actgcttggc ttctatcacc agtacccgca ggactttccc gctggttacg gattctccgg
     3601 catcgcggtg gccttgctcg ggcggaacaa tccggtaggc atggcctttg gagccctgct
     3661 cttcgggttc atgaaccggt cgtcgctgat cctcgacctc aaagatgtgc ccaaggagat
     3721 cgtgaccatc attcagggca tcgtcgtcct ggccgtcgtc atcgtgtacg aagtgatctc
     3781 tcgctacgtg caacgtcgga ccgtcgccgc tgcggcccga gcgacagagg ccgacgaagc
     3841 ggctcaggtg gcggcatgac aacggcagaa gtagtaactc ggcggacacg gcttccggcc
     3901 aggtcgcggc gcattgtgat catcgcgctg gccgggatcc tggtcatgac cattgccgag
     3961 ctgatcacgg ggaccgaaga cctcacctct cggagcactg ccagcgcggc attacggctc
     4021 tcgataccca tcctccttgc cggactcggt gggctctact cggagcgatc gggcgtggtg
     4081 aacatcggtc tcgagggaat gatgattgcg ggcacctggt tcgccgcctg gggctcatgg
     4141 atgttcggcc cgtgggaagg ggtggcccta ggcattgttg gcggtgccat gttcggtttg
     4201 ttacatgccc tggccactgt caccttcaac gtggatcaca tcgtctccgg tgtggcgatc
     4261 aacatcctgg gcctgggtgc gatgcgtttc ctgtcttcgg tcgtctacac cgaggagacg
     4321 ggcggatcgg tgatccagtc gccaccgatc cagggtatcg agaatgtcag ttttcccttc
     4381 ctcgccggcg gccagctgtt cggctggcaa agccctgacg ctctcggttg gctggaggag
     4441 aagagctggt tcttcgtatc cgacatcgcc gggatcatgc gtggcttctt tggtgacgtg
     4501 aactggctga ctctcatcgg gcttgccatc gtccctctct cggcatggct tctctggcgg
     4561 accgcgtggg gccttcgact ccgatcgtgt ggtgaggacc cctgggccgc ggagtcactg
     4621 ggcgtgcccg tgctccggat gaagtattac ggggtcgtca tctcaggcat gctcgccggg
     4681 ctggccggtg ccttcctcgt tctggttcag gccggtatct atcgggaagg gatgagtggt
     4741 ggtcgcggat tcatcgggtt gggggcgatg atcttcggca actggatccc ggttggcgtt
     4801 ctcggtggct
//
LOCUS       DBNHXU010000390         4229 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2143117, whole genome shotgun sequence.
ACCESSION   DBNHXU010000390 DBNHXU010000000
VERSION     DBNHXU010000390.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2143117"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..457
                     /locus_tag="AC161X_09515"
     CDS             <1..457
                     /locus_tag="AC161X_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011756777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HIR4989833.1"
                     /translation="ASEFLALKRIAVTGVSRTPQGHGGNIVYQRLRDRGYEVFAVNPN
                     ADSVEGDDCYQDLKSIPGGVDAVVIGTRPEIADETMKECADLGIEHVWMHRAFGEGSV
                     SDAATEFGRDHGIKVIDGGCPCMFEPTADPAHKMIRFVSTLTGKVPREV"
     gene            688..1383
                     /locus_tag="AC161X_09520"
     CDS             688..1383
                     /locus_tag="AC161X_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015625017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HIR4989834.1"
                     /translation="MRILVVEDEADLAMAIAAGLRQDGYAIDIAESASSAKRFVAVNS
                     YDLVCLDLNLPDGDGLDVCRQIAVGENHDLAPGPAPKIIMLTARGSVADRIRGLDEGA
                     DDYLVKPFSLGELGARVRALLRRDTATGSSLLTFGGIEMDTARHEVTYCGKLVGLTVK
                     EFALLRWFLLHPDEVHSSERLLEHVWDEHADPFTNTVRMTLSNLRRKLAVVGAGSHII
                     TLPGRGYMLRVES"
     gene            1380..2513
                     /locus_tag="AC161X_09525"
     CDS             1380..2513
                     /locus_tag="AC161X_09525"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4989835.1"
                     /translation="MKEALLQNLPGGRSRARLTLLLVSPALLVIVLAIAATSVFLLTN
                     DAGTQDAAKAGLARVGDALAVVIVTGDGGAATIAGTRDLVVVWGLVLPAAALVPAAVA
                     AWLLAGRVLRIIGRANSEVERADDERSNHLQEIVHELRTPLAVMGANLELASTKVDLD
                     SGATPHIGAARRAVDRMARTVDDLDGHGRLSVEQEDGPVDLANLAAGAVAEHAGLAQA
                     GGIHIVLTGAPSTMLPAADPAAVSAAIGNFLSNAVRFAPRGSSVTVDWGEVGDWAWLS
                     VTDEGPGLAPHLHARVFERGWQGPYDRSRGSGSGGAGLGLTIARQLTEAQGGAVTLES
                     EEGGGATLAMWLPLDHEANQDDVIDSDRVHTAIQPWRKDFQPA"
     gene            2691..3755
                     /locus_tag="AC161X_09530"
     CDS             2691..3755
                     /locus_tag="AC161X_09530"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HIR4989836.1"
                     /translation="MATEVQPSVPPTPVAVPEKAPRRWIRVIAYVTSALVLIAALGGY
                     WLVRRADTTTTPTVAAGEAEAGSTAAVASALSPAVVHIEVMGGLEAGGGVGSGVIYDT
                     SGLVLTAHHVVSFSDEVTVITADGRSFNGRVVGRIPERDLAVIALDDASDLVAAAIAE
                     PGTVEVGEPAIALGSPFGYQQSVTAGIISGLDREVETPMGTLTGLIQTDAPINPGNSG
                     GPLADAEAKVIGINTAIASMSGGNDGVGFAVPIEDFASLIDEVQANGGVDAPTIPAPE
                     DASSMQGLVPGLEDLLPGLEDLFGPGGAQDLQDLPDLQEMLDQMFGPGAIPPELEQLL
                     EELLSIPAPTQTPDEETPGA"
     gene            3755..>4229
                     /locus_tag="AC161X_09535"
     CDS             3755..>4229
                     /locus_tag="AC161X_09535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018731711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989837.1"
                     /translation="MTTPLLPTVDGPQSDLLATRLRQVMTELKTVIVGQDRVLERVLV
                     ALLAGGHCLLEGVPGLGKTLTVSTLARTLGGTFTRIQFTPDLLPSDIVGTRIYRPSTE
                     GFDVELGPVFANFVLADEINRAPAKVQSALLEAMGERQISIGGTTYRLPLPFLVLA"
BASE COUNT          747 a         1418 c         1311 g          753 t
ORIGIN      
        1 tgcctctgag ttcctcgcct tgaagcggat agccgtgacc ggagtgtcgc ggaccccgca
       61 agggcacgga ggcaacatcg tctaccagcg gcttcgggac cgcggctatg aggtgttcgc
      121 tgtcaacccg aacgccgact ctgtcgaggg tgacgactgc tatcaggatc tgaagtcgat
      181 tcctggcggt gtcgatgcgg tggtgatcgg aaccagaccc gaaatcgcag acgaaaccat
      241 gaaggaatgc gccgacctgg gaatcgagca cgtttggatg catcgtgcct tcggtgaggg
      301 aagcgtgtcc gacgcagcga ccgaattcgg ccgggaccac gggatcaagg tgatcgacgg
      361 gggctgcccc tgcatgttcg agccgaccgc agacccggcg cacaaaatga tccgcttcgt
      421 gtcaaccctc accggcaagg tcccccgcga ggtctgaacc gggggactag gtccacctcg
      481 acggtcgcaa cagagaaccc aaatcctggc cacttggagg cgggttctca ttgccctgcc
      541 gtcctgtcat cctgagagtt ctggacccca tcgggaactc tcccgacatg ttcaacgtca
      601 tatcaagggc tccccgcccc gctccatgat ccttcacctc ctaaccccgt tcccacgcgt
      661 cgccgcgccc atcacggtgg cccgctgatg cgcatcctcg tagtcgaaga cgaggccgac
      721 ctggccatgg ccatagccgc cgggttgcgt caggacggat acgccatcga tatcgctgaa
      781 agtgccagct ccgccaagag attcgttgcc gtcaactcat acgacctcgt ctgcctcgac
      841 ctcaatctgc ccgatgggga cggcctagat gtctgccggc agatagcagt aggggagaat
      901 cacgacctcg ctcccggccc tgccccgaag atcatcatgc tcaccgctcg ggggagcgtc
      961 gccgatcgga ttcgcgggtt ggacgagggc gccgacgact atctggtgaa gccgttctcg
     1021 ctgggcgagc tcggagcccg ggttcgtgcc ttgttgcgac gggacaccgc caccggcagc
     1081 tcactgctca ctttcggcgg tatcgaaatg gacacggctc gccatgaggt gacctattgc
     1141 gggaagctcg tcgggctcac cgtcaaggag ttcgcccttc tccgttggtt cctccttcac
     1201 cctgacgagg tgcacagcag cgagcgtctc ctggagcacg tttgggacga gcacgctgac
     1261 cccttcacca acacggtcag aatgaccctg tccaacctcc gtcggaagct tgcggtggtc
     1321 ggagcgggat cacacatcat cacactgccc ggtcggggct acatgctgcg ggtcgagtct
     1381 tgaaagaagc gctcctgcag aacctgccgg gtggacggag ccgggcaaga ctgaccttgc
     1441 tcctcgtttc gccagcgctg ttggtgatag tcctcgctat cgccgcgact tcggtctttc
     1501 tgctgaccaa cgatgccgga acccaagacg ctgccaaggc cggtctggcc cgggttggcg
     1561 acgctctcgc cgttgtgatc gtcaccggcg acggcggcgc agccacaatc gcggggacca
     1621 gggatctcgt cgttgtctgg ggtcttgttt tgccggccgc agcactggtt ccagcggcgg
     1681 tcgccgcatg gctgctggcc ggacgcgtcc tgcggatcat tggccgtgcc aactccgagg
     1741 tggaaagggc cgatgacgaa cgcagcaacc acctgcagga gatagtccac gagttgcgga
     1801 ctcccctggc agtgatggga gccaatctcg aactcgcatc caccaaggtc gacctcgact
     1861 cgggtgccac tcctcacatc ggcgcggctc gtcgggcggt cgatcgaatg gctcgcaccg
     1921 tcgacgacct cgacgggcac ggtcgactgt cggtcgagca ggaagacggc ccggtggatc
     1981 tcgcaaacct ggccgcaggc gccgtggccg aacatgccgg tcttgctcag gcgggtggga
     2041 ttcacatcgt cctcaccgga gcaccctcga ccatgcttcc ggcagcggac ccggcggcgg
     2101 tgtcagctgc gatcgggaac ttcctgagca acgcggtgcg gttcgcgccg cgcggctcct
     2161 cggtgaccgt cgactggggc gaggttggcg actgggcttg gctctcggtc accgacgaag
     2221 gaccagggct ggcgccacac ctccacgctc gagtctttga gcgaggatgg cagggaccat
     2281 acgaccggag tcgtggttcc ggcagcggtg gagcgggcct cggtctgacc atcgcccgac
     2341 aactcacaga agctcaaggc ggtgcggtga ccctcgaatc ggaggagggc ggaggggcca
     2401 cacttgccat gtggctgccc ctcgaccacg aggcaaacca agacgacgtt atcgactccg
     2461 atcgagtgca caccgcgatc cagccttggc gcaaagattt ccagccggct tgacggcccc
     2521 tttaaaccgc gtttcgtatc ttcccgcggt ttaccccaat cggaggcctc ttgactcagc
     2581 tcgacccaaa tcccccaaag cttcgttcgc gtcgtcgatc gtggcgtgac cttccggcgt
     2641 ctccgccggt gtccgacctg attgcgccct ccacaaatac gagcgaccag ttggcgaccg
     2701 aagtccaacc gtcggtcccg ccgacgcccg tcgcggtccc cgaaaaggca ccgaggcgct
     2761 ggattcgggt cattgcctac gtcacgtccg ccctggttct catcgcggcg ctcggtgggt
     2821 actggctggt acggcgagcc gacaccacca cgaccccgac cgtggcggcc ggcgaagccg
     2881 aggccgggag cacggctgcg gtggcttccg ccctcagccc ggctgtggtc catatcgaag
     2941 tcatgggcgg gctagaagcc ggcggcggcg tcggttcagg agtgatctac gacacatccg
     3001 gcctggttct caccgcacac cacgtggtct cgttctccga cgaggtcacc gtcatcacag
     3061 ccgatgggcg gagtttcaac ggccgagtgg tcgggcgcat ccccgagcgt gacctggcgg
     3121 tcatcgccct cgatgacgca tccgacctgg tggcggcagc aatcgccgag ccaggaacgg
     3181 tggaagtcgg cgagccagcc attgccctcg gaagcccgtt cggctaccag caatcggtga
     3241 ccgcgggcat catctcgggt cttgacaggg aggtggagac ccccatgggc accctcaccg
     3301 gcctcattca gaccgacgca ccgatcaatc cgggcaactc gggcgggccg ttggccgacg
     3361 ccgaagccaa ggtcattgga atcaacaccg ccatcgcctc gatgtccggc ggcaacgacg
     3421 gagtcggatt cgcagttcca atcgaggatt tcgcctcact gatcgacgag gtccaggcca
     3481 acggtggggt cgacgcccca accatccccg ccccagagga cgcctcgagc atgcagggat
     3541 tggtccctgg tctcgaggat ctgctccccg gtctcgagga tctgttcggt cccggtggcg
     3601 cacaggacct tcaggatctc cccgaccttc aggagatgct cgaccagatg ttcggcccgg
     3661 gtgcgatccc gcctgagctg gaacagttgc tcgaagagct tctgtccatc cccgcaccga
     3721 cccagacgcc cgacgaggag acaccaggag catgatgaca accccacttc taccgaccgt
     3781 ggacgggccg cagtccgacc tcctcgccac ccggctccgc caggtcatga cggagctgaa
     3841 gaccgtcatc gtcggccagg accgcgttct cgaacgtgtc ttggtggcgc tgctggccgg
     3901 cggccactgc ttgctggaag gtgtgcccgg actgggtaag accctcacgg tttcgaccct
     3961 cgccaggacg ctcgggggca ccttcacccg aatccaattc accccggacc tgctgccatc
     4021 cgacatcgtc ggcacccgca tctatcgacc ctcgacggag ggcttcgatg tggagttggg
     4081 cccggtgttc gccaacttcg tactcgccga cgagatcaac agggctccgg ccaaggttca
     4141 atcggccctc ctcgaggcca tgggtgagcg tcaaatctca atcggcggta ccacctaccg
     4201 actcccgctt cctttcctgg ttctggcca
//
LOCUS       DBNHXU010000391         2886 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3532500, whole genome shotgun sequence.
ACCESSION   DBNHXU010000391 DBNHXU010000000
VERSION     DBNHXU010000391.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3532500"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..432)
                     /locus_tag="AC161X_09540"
     CDS             complement(<1..432)
                     /locus_tag="AC161X_09540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989838.1"
                     /translation="MRRLALMVAALVVTAACSSESVAPFGILASSQPIGIGEQRVMVG
                     LVDQDSGEYLASPDNPATVTLRNEDGSPIETYPTEFMWTAEEVRGLYVARMDIPEAGT
                     YQLTLDAEGFSTAGPMGLVAVDAPGVIQAGDPAPASETRVAA"
     gene            complement(429..1352)
                     /locus_tag="AC161X_09545"
     CDS             complement(429..1352)
                     /locus_tag="AC161X_09545"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018686123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HIR4989839.1"
                     /translation="MATDLPGPPAFPEVAVSIPHDHPRSKVAAYVALTKPRIIELLLI
                     TTVPAMVLAAGEWPGAWILVTTLIGGTLSAGGANALNCYVDRDIDELMRRTKGRPLAR
                     HEVPPSNALVFGICLGVAGFFWLWVTTNLLAASISTAALLFYVFVYTIALKRSTTQNI
                     VIGGAAGAAPVLVGWAAVTGEIALPAWVLFFVVFYWTPPHFWALAIRYKDDYARAGVP
                     MLPVVVGVEATTRKMLLYTALMVGITLLLVPVAGMSWIYLVAAIGLGAWFLWDSWRVY
                     LHPEQAMRLFTTSTVYLAALFASVMLDVLIR"
     gene            complement(1342..1512)
                     /locus_tag="AC161X_09550"
     CDS             complement(1342..1512)
                     /locus_tag="AC161X_09550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989840.1"
                     /translation="MRFIFAHYWGIDEVGIFVIPALAAIWSLRWAERRARRNAEERKL
                     AEEPLEEPSDGD"
     gene            complement(1509..>2886)
                     /locus_tag="AC161X_09555"
     CDS             complement(1509..>2886)
                     /locus_tag="AC161X_09555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989841.1"
                     /translation="RDPSHPLMRRRAGVVGGALLLILLIPGTALAHGIGGRLDLPVPV
                     TYFIAAAGVVIVISFVALALLWPEPRLQGGPRQEPGRLTVPRRGLLPVLGVIGLLLVI
                     GQLVVAAFGLTTDPTRPSIAPVLIWVVFWLIIPFTGALIGDWYSDLNPWRTIGRTFKI
                     GRAERLWLRDAVGVLPAAALLLSFTWLELVNHESGSATALGWAALAYTVVMLGTMAYA
                     GLETGLTVFDAFTPYNRLISSISPLGRRADGKLVWRGWLRALTVLPEWRGLALLVCVA
                     IGTVTYDGASGTSWFKAAFGTMAESVPGGTLLLLASVALIAVAYWLASWVAARMVGGD
                     WTANRVSARFAHTLVPIALAYAVAHYMTLIIFEGQQLIAAVSDPFALGWDLFGTADRK
                     IDFFITTSEPIWYLQLAVIVTGHVTGVVLAHDRALGDFGPDAVRSQYAMLLLMVALTT
                     LGLLILAG"
BASE COUNT          645 a          897 c          931 g          413 t
ORIGIN      
        1 ggccgcgacc cgggtttcgg aagccggagc agggtcgccg gcctgaatga cacccggagc
       61 gtcaacggcc accaggccca tcggccccgc cgtcgagaac ccttcggcat ccagggtcag
      121 ctgataggtg ccggcttcgg ggatatccat ccgggccacg tagagaccac gaacctcctc
      181 cgcggtccac atgaactcgg tggggtaggt ctctataggc gagccatcct cgttgcgaag
      241 ggtgaccgtc gccggattat cgggagaagc caggtactcg ccgctgtcct ggtcgacgag
      301 accaaccatg acccgctgtt ctccgatgcc gattggctgc gaggaggcaa gaatgccaaa
      361 cggggccacg gattccgagg agcaggccgc cgtgaccacc aaggccgcca ccatgagtgc
      421 cagtcgcctc atcggatcag cacgtcgagc atcaccgaag caaagagagc cgccaggtac
      481 accgtggaag tggtgaacag gcgcatcgcc tgttcggggt ggagatagac cctccatgag
      541 tcccagagga accaggcgcc gagcccgatc gctgccacca ggtagatcca actcatcccg
      601 gctaccggga ccaacagcag tgtgatcccg accatgagcg cggtgtagag gagcatcttg
      661 cgggtcgtgg cctcgacacc taccaccacc gggagcatgg ggaccccagc ccgggcgtaa
      721 tcatccttgt aacggatggc gagggcccag aagtggggag gcgtccagta gaagacgacg
      781 aagaagagca cccacgccgg cagcgcgatt tcaccggtga ccgctgccca accaacgagt
      841 accggtgccg ccccggccgc gcctccgatg acgatgttct gggtggtcga gcgtttcaac
      901 gcaatggtgt atacgaagac gtagaaaaga agagcagcag tggagatcga cgccgcgagg
      961 aggttggtcg tcacccagag ccagaagaag ccggcaacac caaggcagat gccgaacacc
     1021 agggcgttgg acggggggac ttcgtgccgg gccagcgggc ggcccttggt tcgacgcatc
     1081 aactcgtcga tgtcccggtc gacatagcaa ttgagggcgt tggcgccgcc cgcggagagt
     1141 gtgcctccga tcagtgtggt gaccaggatc cacgctccgg gccactcgcc agcagcgagg
     1201 accatggccg gcaccgtggt gattagaagc aactcgatga tccgtggctt ggtcagagcg
     1261 acataggcgg cgaccttgga tcggggatga tcgtgcggaa tcgagacggc cacctcagga
     1321 aaagccggcg ggccggggag gtcagtcgcc atcggatggc tcctcaaggg gttcttccgc
     1381 cagctttctc tcctcggcgt tgcgacgtgc ccggcgctct gcccatcgca acgaccagat
     1441 agcagccagg gccgggatca cgaagatccc aacctcatcg atcccccaat agtgggcaaa
     1501 tatgaatctc atccggccag gatcaacaat ccgagtgtgg tgagagccac cataaggaga
     1561 agcatcgcgt attgggaacg gaccgcgtcc ggtccgaagt ctccaagagc gcgatcgtgg
     1621 gcgagaacta cgcccgtcac atgcccggtc acgatgactg cgagctgcag gtaccagatc
     1681 ggctcgctcg tagtaatgaa gaagtcgatc ttgcgatcgg cggttccaaa caagtcccag
     1741 ccgagggcga acggatcaga cacggctgcg atcaattgtt ggccttcaaa gatgatcagc
     1801 gtcatgtagt gagccaccgc ataggcgagg gcgatcggga ccaacgtgtg ggcgaatcgg
     1861 gccgagaccc gatttgcggt ccaatcccca cccaccatcc gggccgcgac ccaagaggcc
     1921 agccagtagg cgactgcgat cagcgccacc gaggccagca ggagcagggt ccctcccgga
     1981 accgattccg ccatggtccc gaacgcggcc ttgaaccagg aggtgccgga agctccgtca
     2041 taggtcacgg tgccgatcgc aacgcagacc agcagagcaa ggccacgcca ttcagggagg
     2101 acagtcaacg cccgcagcca gccacgccag acgagttttc cgtccgcacg gcgacccaga
     2161 ggtgagatcg aggaaatcag cctgttgtaa ggagtgaagg cgtcaaacac agtcagcccg
     2221 gtctctaacc ccgcataagc catcgtcccc aacatgacca cggtgtaggc cagcgccgcc
     2281 cagccgaggg ccgtcgccga tcccgactca tggttgacca actccagcca ggtgaacgac
     2341 aagaggagtg cggcggcagg gagaacgcca acggcatcgc gcagccacag gcgctcggcg
     2401 cgtccgatct tgaaggttcg cccgatggtg cgccacgggt tgagatcgga gtaccaatca
     2461 ccgatcagag cccccgtgaa gggaatgatc agccagaaga cgacccagat cagcaccgga
     2521 gcaatcgagg ggcgggtggg gtcggttgtg aggccaaagg cagccaccac cagctgcccg
     2581 ataaccagta gcaagccgat gacacccaac accgggagca acccccgacg aggaactgtg
     2641 agccgtcctg gttcctggcg ggggcctcct tgaagcctgg gctcgggcca gagcagtgcc
     2701 agggccacga agctgatcac gatgacgaca cctgcggctg cgatgaaata ggtgacggga
     2761 accggcaggt cgagacgacc cccaatgcca tgggccaggg ctgtccccgg aatgagaaga
     2821 atcaggagca gagccccgcc gacgacgccg gcgcgacgtc tcatcagggg gtgacttgga
     2881 tctcga
//
LOCUS       DBNHXU010000392         2821 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3335646, whole genome shotgun sequence.
ACCESSION   DBNHXU010000392 DBNHXU010000000
VERSION     DBNHXU010000392.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3335646"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(44..763)
                     /locus_tag="AC161X_09560"
     CDS             complement(44..763)
                     /locus_tag="AC161X_09560"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046873 - metal ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR4989842.1"
                     /translation="MTSDSESLPYEPHIGESRQYWRDIILGVNDGLVSIYLLVVGVAG
                     GGLDTSQVLLTGLAGALAGAVSMAAGEYLATKSQDEVLEAELALERVHIKDHRGMEVG
                     QLRDMFADMGIAESDLDGVVTAFASSDEAMLNAMKALEFGFVESERRSPYRAMFASGG
                     LFLVGSLSSILPFMIFDTNTPALIWASILSLGGLFLVGVVKARVTRNNWVRSGLENMV
                     IAGIGGLLAWLIGTAIGGALD"
     gene            complement(753..1910)
                     /locus_tag="AC161X_09565"
     CDS             complement(753..1910)
                     /locus_tag="AC161X_09565"
                     /inference="COORDINATES: protein motif:HMM:TIGR01891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HIR4989843.1"
                     /translation="MTDPERVAGLAGEITPLALDLRRLLHRHPEGAHEERHTTELIAT
                     ALDESGISYHLRAPRTGLWVDIGDRPTIGFRADLDALPINEPESNDPISERPGWMHAC
                     GHDAHAAIAFGIAAVLDRMDLPSGIRILFQPAEESFPGGAVELVEEGLVDGLKGLIAF
                     HIDPTLATGRIGSRPGPNTASADKFTLVLEGPGGHTARPHRTVDLIADAARLVHELPG
                     WMRRTVDARSPLTVAFGSIHGGEAGNVIPTRVELEGTVRTLDGDLWGALPGLVDKAVS
                     SLLAVSDATYELTYSQAIPPVVNDVGVVESAVSGIVGMLGRESVVPTEASMGGEDFAH
                     YLTRVPGALFRLGGGGSGGDLHSPSFQLDEAAIGFGIQAGVAALLGMQSDI"
     gene            complement(1927..>2821)
                     /locus_tag="AC161X_09570"
     CDS             complement(1927..>2821)
                     /locus_tag="AC161X_09570"
                     /inference="COORDINATES: protein motif:HMM:NF019935.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase family
                     protein"
                     /protein_id="HIR4989844.1"
                     /translation="TDSADAAVAGALHIATEADPTGFAVVAMGKWGGGELSYGSDLDV
                     MYVFDPESERDEGLRIAAYLSKVISEPNRHGEAYMLDAEIRPEGRRGPLARSVDSYRR
                     YYEEWAEPWEVLALVRARPAAGDDKVAVAFASVIEPVVWRENLSESFIRSIRDIKARV
                     EKERIPPGEDPDFHLKLGKGSLSDIEFLTQILQIRHGFAIPALRVTGTLDALAQLRDH
                     EVLTGVEEETLRESYIFCTRVRLRLHLQAGRASDSLPTDSDELIRLASSLGYPRAAEL
                     RDQYRRVTRRARSVFERRFYE"
BASE COUNT          512 a          916 c          896 g          497 t
ORIGIN      
        1 agctaactgc taactattgc aagcacgacg cgtgcaggcg taactagtcg agggcgcccc
       61 cgattgcggt gccgatcagc caggcgagaa ggccaccgat tccggcgatc accatgttct
      121 ccagaccgga gcgaacccag ttgttgcgag tcacccgggc ttttaccacc cccaccagga
      181 acagaccccc gagtgacaga attgaggccc agatcagggc tggcgtgttg gtgtcgaaga
      241 tcatgaaggg aaggatcgaa gacagggaac cgaccaggaa gaggccccct gaggcgaaca
      301 tcgcccgata tggcgagcgg cgctctgatt cgacgaaccc gaactcgagt gccttcatcg
      361 cgttgagcat cgcctcgtcg gagctggcaa aggcggtgac cacaccgtcg aggtcggact
      421 cggcgatccc catgtccgcg aacatgtccc ggagttggcc gacctccatg cctcggtggt
      481 ctttgatgtg aacccgttcc agagcgagct ctgcttcgag gacctcgtcc tgcgacttgg
      541 tcgccaagta ctcgccggcc gccatggaga cggctccggc aagcgccccg gcaagacctg
      601 tcaggagcac ctgactggtg tcgagaccac ctccggcgac ccccaccacc agcagataga
      661 tcgagaccag cccgtcgttg accccgagaa tgatgtctcg ccagtactgc ctggactccc
      721 cgatgtgggg ctcgtacggc agggactcgc tgtcagatgt cactctgcat ccccaacagt
      781 gcagccacgc ccgcctggat cccgaacccg atcgcagcct cgtcgagctg aaacgagggt
      841 gagtgaaggt cgccaccgga tccgccgcca ccgaggcgga acagggctcc cggaacccgg
      901 gtcaggtagt gagcgaagtc ttcgcccccc atgctcgcct ctgtcgggac caccgactcc
      961 cggccgagca tcccaacgat ccccgagacg gcggactcca cgacaccaac gtcgttgacg
     1021 accggcggga tggcctggga gtaagtgagc tcataagtgg cgtccgaaac cgcgagcagt
     1081 gagctcactg ccttgtcgac aagacccggg agggcccccc acaggtcacc gtcgagggtg
     1141 cgaaccgttc cctcgagctc gacgcgggtg gggatcacat tgcctgcttc tcctccatgg
     1201 atcgatccga atgcgaccgt aagtgggctt cgggcgtcga ctgtccggcg catccagccc
     1261 ggaagctcat gcaccaaccg ggcagcgtcg gcgatgaggt cgaccgtccg gtgaggtctc
     1321 gcggtgtggc caccgggtcc ctcgagtacc agggtgaact tgtcggcgct cgccgtgttc
     1381 ggccctggcc ggctcccgat tcggcccgtc gcaagagtcg ggtcgatatg gaaggcaatg
     1441 agacctttga gcccgtcgac cagaccctcc tccaccagtt cgacggctcc gcccgggaag
     1501 gactcctctg ccggttggaa caggattcgg atcccggatg gaaggtccat tcgatcgagg
     1561 actgccgcga taccgaacgc gatcgcggcg tgcgcatcgt gcccgcaggc gtgcatccaa
     1621 ccgggtcgct cagagatcgg atcgttcgac tcgggctcgt tgatggggag ggcgtccagg
     1681 tccgcccgga aaccgatcgt cgggcggtcc ccgatgtcta cccatagccc ggttcgagga
     1741 gcccttaggt ggtagctgat gccactctcg tccagagccg tcgcgatcag ttctgtcgta
     1801 tggcgctctt cgtgagcacc ttcagggtgc cggtggagca ggcggcgaag atcgagagca
     1861 agaggcgtga tctcgccggc cagcccggcg acccgctcgg gatcggtcat tattcgaggt
     1921 taccggtcac tcgtagaacc tcctctcaaa cacgctccgc gctcgcctcg tcactctcct
     1981 gtattggtct cgcaactcgg cagctcgcgg ataacccagc gagctggcga gccggatcaa
     2041 ctcgtcggag tcagttggca acgagtccga ggctcgaccc gcctggaggt ggagacggag
     2101 ccggaccctg gtgcagaaga tgtacgactc ccgcagggtc tcttcttcga ccccggtgag
     2161 aacctcgtgg tcacggagtt gggccaaggc gtccagggtt cccgtcaccc ggagggcggg
     2221 aattgcgaac ccgtgtcgga tctggaggat ctgggtgagg aattcgatgt cgctcaggct
     2281 gcctttgccc agcttgaggt gaaagtcggg gtcctccccc ggtgggatgc gctccttttc
     2341 cacccgggct ttgatgtcgc ggatcgatcg gatgaaggat tcggagaggt tctcgcgcca
     2401 gacgaccggc tctatcaccg aggcgaatgc gaccgcgacc ttgtcgtcgc ctgccgccgg
     2461 gcgtgcccga accaatgcga ggacctccca cggctcggcc cactcttcgt agtaacggcg
     2521 atagctgtcc actgaccggg caagcgggcc tcgccggcct tccgggcgta tctcggcgtc
     2581 caacatgtag gcctccccgt gccgattcgg ctcggaaatg accttggaaa ggtaggcggc
     2641 tatgcggagt ccctcgtcgc gctccgattc cggatcgaag acatacatga cgtcgaggtc
     2701 cgaaccgtat gagagctcgc ctccccccca tttgcccatg gcgaccacgg cgaacccggt
     2761 gggatcggct tcggtggcga tgtgcagggc accggcgact gctgcatcgg cgctgtctgt
     2821 g
//
LOCUS       DBNHXU010000393         1747 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5122807, whole genome shotgun sequence.
ACCESSION   DBNHXU010000393 DBNHXU010000000
VERSION     DBNHXU010000393.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1747)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1747
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5122807"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..471
                     /locus_tag="AC161X_09575"
     CDS             <1..471
                     /locus_tag="AC161X_09575"
                     /inference="COORDINATES: protein motif:HMM:NF047172.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR4989845.1"
                     /translation="DRKLDFDALLAVSNIYRAANAVRNRMEREVLAPEGLSWGGFTIL
                     FVLWVWGERETGELADDCGLAKGTLSGMLGTLEKTGLVSRSRHPGDGRRVIVVLEADG
                     RELIERVFPRFNEHETKFTSRLGPTEQGELARLLRIVTATADDLPLLADPQVKA"
     gene            509..1384
                     /locus_tag="AC161X_09580"
     CDS             509..1384
                     /locus_tag="AC161X_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006383696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DODA-type extradiol aromatic ring-opening family
                     dioxygenase"
                     /protein_id="HIR4989846.1"
                     /translation="MGEIVGAGLISHAPTIMFDEATRLELNEGKEISLVPGLVRLRDE
                     VLNRLEADTIVIVDSHWFSTVEHLVSAHARRHGIFTSSELPRGMAQIPYDYPGDPELA
                     KLAADLGSELGTPTHANEDDYLPVYYATINLVHYLRKDENVVSVSVVQTGDTDDFMTM
                     GEAIGEAVTRLDRRAVILASGGMSHTFWPLKEIAKHEASDPVHIITPAARAADEERLE
                     MMLAGDHARVVDTMDDYFEYSPEGAFGHYLMMLGAIGGRNCAAPGEMFSDYENATGTG
                     QVHVWFERPSGGWTA"
     gene            1427..>1747
                     /locus_tag="AC161X_09585"
     CDS             1427..>1747
                     /locus_tag="AC161X_09585"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR4989847.1"
                     /translation="MALNAPDDLISELEELACSVDNLVFGGDPAGQAHAKDRDRIVRT
                     IRSYAIPRMTLPATPLLVVFAGPTGAGKSTLVNSVSGLEVAETGPLRPTTRAPVVLAA
                     DGSGY"
BASE COUNT          311 a          573 c          554 g          309 t
ORIGIN      
        1 gatcggaaac tcgacttcga cgccctgctc gccgtgtcca acatctatcg cgctgccaac
       61 gcggtgcgca atcggatgga aagagaagtc ctcgccccgg aagggctgtc ctggggcggg
      121 ttcacgatcc tgttcgtgct ctgggtgtgg ggggaacggg agacgggtga gcttgccgac
      181 gactgcgggc tggcgaaggg aactttgagc gggatgctcg gcacccttga gaagacgggc
      241 ctggtgtcac ggtcccgtca ccccggcgac ggtcgccggg tgattgtcgt tctcgaggcc
      301 gatggccggg agctgatcga gcgggtcttt cccaggttca acgagcatga gaccaagttc
      361 acgtcgagac tcggcccgac cgagcaggga gagttggcgc ggctgctccg catcgtgacc
      421 gccaccgccg acgatctgcc actcctggcc gacccccagg taaaggcgta aagtctcatt
      481 tggttcctaa cgacctccag gaggacccat gggtgagata gtcggcgccg gtctgatcag
      541 tcacgcacca accatcatgt tcgacgaggc aacccgcctc gagctcaacg agggcaaaga
      601 gatctcattg gtgcccggac tggttcggct gcgcgacgag gtgctgaatc gccttgaggc
      661 cgacacgatc gtgatcgtcg actcgcactg gttttccact gtggagcatc tggtatctgc
      721 ccacgcccgc cgtcacggca tcttcacctc ttccgagctg ccccgtggga tggcccagat
      781 cccctacgac tacccgggcg accctgagtt ggccaagctt gcggccgacc ttggctccga
      841 gttaggcaca ccgacccacg ccaacgagga cgattatctg ccggtctatt acgcgacgat
      901 caacctcgtc cactatctgc gcaaggacga gaatgtggtc tcggtgtcgg tcgtgcaaac
      961 cggggacacc gatgacttca tgacgatggg tgaggccatt ggggaggcgg tcacccgcct
     1021 cgaccggagg gcggtgatcc tggcctcggg agggatgagc cacaccttct ggcccctcaa
     1081 ggagatcgcc aagcacgagg cgtctgatcc ggtgcacatc atcacacctg ccgcccgcgc
     1141 tgccgacgag gagcgcctcg agatgatgtt ggccggcgac cacgcccggg tggtggacac
     1201 catggacgac tacttcgaat actcgccaga gggcgccttc ggccactacc tgatgatgct
     1261 cggcgccatc ggtggccgga attgcgcggc accgggggag atgttctccg attacgagaa
     1321 cgccaccggc actggccagg tccatgtctg gttcgagcgc ccctcggggg gctggaccgc
     1381 ctgaccacga tcagggggct ccccgtgcca caatttcacc gaagtggtgg cactcaacgc
     1441 gcccgatgac ctgatctccg agctcgaaga gctcgcatgc tccgtggaca atctcgtctt
     1501 cggcggcgac cccgccggac aggcgcatgc caaagaccgg gaccgcatcg tgaggaccat
     1561 ccggtcctac gcgatcccgc ggatgactct tccggcgaca ccgctcctgg tggtcttcgc
     1621 agggcctacc ggtgccggga agtccaccct cgtcaacagc gtctccggtc tcgaggtcgc
     1681 ggagaccggc cccctccgcc caaccaccag agctcctgtg gtcctggccg ctgatggctc
     1741 cggctac
//
LOCUS       DBNHXU010000394         4216 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1747823, whole genome shotgun sequence.
ACCESSION   DBNHXU010000394 DBNHXU010000000
VERSION     DBNHXU010000394.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4216)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4216)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4216
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1747823"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="AC161X_09590"
     CDS             <1..988
                     /locus_tag="AC161X_09590"
                     /inference="COORDINATES: protein motif:HMM:TIGR01976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HIR4989848.1"
                     /translation="NADPAEVSFGQNMTSITFAVSRALATTWKPGDAIVVTSLDHDAN
                     FTPWVRAAGDVGVEVRIAEFDTASGELDPDVIGRLLDDRVRLVAVCVASNAIGTVVDV
                     QGVASLAHETGALVYLDAVHAGPHRLLDVQAFDCDFLVASAYKFFGPHTGILYGKLDR
                     LAGLDAYKVRPAPSDPPGKLETGTQSFESMAGVTAAVDYLASLSGKDGGSRRDRLQDA
                     YSAIGDHERTLSERFLSGLESVPGVRVYGVPIADQRRVATFAIGIEGHSPDSVAAHLA
                     RAGVYVWSGHYYAVNAIDRLGVLDQGGLVRIGFVHYNTLEEVDRALEALTDL"
     gene            complement(993..1175)
                     /locus_tag="AC161X_09595"
     CDS             complement(993..1175)
                     /locus_tag="AC161X_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016330222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system antitoxin"
                     /protein_id="HIR4989849.1"
                     /translation="MTDRKSFLLRINPKLHRVLERWAAEDLRSLNSQIEFLLVDAARR
                     NGKWVKEDPGPDRDEV"
     gene            complement(1172..2062)
                     /locus_tag="AC161X_09600"
     CDS             complement(1172..2062)
                     /locus_tag="AC161X_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020128021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HIR4989850.1"
                     /translation="MTERQSMSAERLVEGFSGWLMASIALILWLGGVIAFVVALSQDD
                     GWLIAAAGLVFLIGAFIVPGLFINQPNQSRVVTLFGHYSGTVRKNGFRWVNPLTQRRQ
                     VSLRIRTFDSDVLKVNDAVGNPVEIASVINWQVVDTARAAFDVEDYEHFVKVQSEAAI
                     RHVASEYPYDHIEDEGPSLRANADDVTATLQSELQDRLDEAGVTIRDCRLRRLAYAPE
                     IAGEMLRRQQAGAVIAARKLIVRGAVTMVQDALEQLAAEHIVELDEERKAAMVSNLMV
                     VLTGDRSVQPVVNAGTLYPG"
     gene            complement(2094..2720)
                     /locus_tag="AC161X_09605"
     CDS             complement(2094..2720)
                     /locus_tag="AC161X_09605"
                     /inference="COORDINATES: protein motif:HMM:NF024859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4989851.1"
                     /translation="MAKSDLVIDHVIFCAPDLDQASDWFLRRHGLRSVAGGRHAGLGT
                     ANRIIPLGDNYIELLTVVDRRAARANPFGMWASVSIEEKSMLPVGLCMRTGDIASVTK
                     RLQLKPTAMSRTTPDGIELRWQLAGLEQTLNRGHPFFIQWDIDMPDHPGRLLEGQGPM
                     EKLTLSGRAEELRGWLGANTDEVAVVDGPSEITRLTISHPSGSIELGY"
     gene            2895..3530
                     /locus_tag="AC161X_09610"
     CDS             2895..3530
                     /locus_tag="AC161X_09610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989852.1"
                     /translation="MIVGVLITGDTAVRAAHSLSAHPSVDEVVVVGPARSKSFRVVPS
                     AEECDFLIGTGPDAPSLARGHGVELIWDGETLEEGVVVAGCSPAGMALAIAARESDPR
                     LVAVAHPDFEEGSGHVVRFPDPVGAAPVADSTLANRRVATAKSPNRFAACLAVSAGRS
                     VTIVDDGAFLSGIALAAGIDVAQEEPKLAFDDALTYLETAAAMGLLMAGDR"
     gene            complement(3584..4084)
                     /locus_tag="AC161X_09615"
     CDS             complement(3584..4084)
                     /locus_tag="AC161X_09615"
                     /inference="COORDINATES: protein motif:HMM:NF023511.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3566 domain-containing protein"
                     /protein_id="HIR4989853.1"
                     /translation="MATVRRVRRIIRKIDPWTALKVSAVVWAVVAIAMVLGTVIFWSV
                     FERAGIPAKLTAFMVEITLIDEGAQPFANVEQFFRVVVFGSVVWWVLATGATVLMAIT
                     YNLVSDVVGGLEVVVLEETLNPVVMPQQVLHPPTDYRPTNGSVAPSDQADIPTEETAP
                     VTVPLT"
BASE COUNT          745 a         1364 c         1296 g          811 t
ORIGIN      
        1 caacgccgat ccagccgagg tgtctttcgg tcagaacatg acgagcatca cgttcgcggt
       61 cagccgtgcc ctggccacta cctggaagcc tggtgacgcg atcgttgtca ccagcctcga
      121 tcacgacgcc aacttcacac cctgggttcg tgctgcggga gacgtcgggg tcgaggttcg
      181 gattgccgaa ttcgacaccg ccagcggtga gctcgacccg gacgtcatcg gacggctcct
      241 cgatgacaga gtccgtttgg tggcggtgtg tgttgcctcg aacgccatcg gcacggtggt
      301 cgacgtgcag ggcgttgcat cactcgcaca cgagaccgga gcgctggtct atctcgacgc
      361 ggtccacgcc gggccccatc ggcttctgga tgttcaggca ttcgactgtg atttcctggt
      421 ggcatcggcc tacaagttct tcgggcctca caccgggatt ctctacggca agctggatcg
      481 tctggccggc ctcgacgcat acaaggtgcg gccggcaccc tccgacccac ctgggaagct
      541 ggagacgggg acccagtcat tcgagtcgat ggccggagtc accgccgcgg tcgactacct
      601 ggccagcttg tccggcaagg acggcgggtc acggcgggat cgtctccagg acgcctattc
      661 cgcgatcggg gaccacgagc gcacgctctc cgagcgcttt ctctccgggc tggagtcggt
      721 gcctggagtc agggtttacg gggtgccaat cgccgatcaa cggcgggtcg ccaccttcgc
      781 cattgggatc gagggacact cccctgattc ggtggccgcc cacctcgccc gagccggcgt
      841 ctatgtctgg tcaggtcact actacgcggt gaatgcgatc gaccgcctcg gagtcctcga
      901 tcagggcggc ctggttcgca tcggatttgt tcactacaac accctcgagg aggtcgaccg
      961 ggccctcgag gccctgaccg acctgtaggc gttcagacct cgtctcggtc cgggcccggg
     1021 tcctctttga cccacttccc attcctgcgg gccgcgtcga ccaagaggaa ctcgatctga
     1081 ctgttgaggc tgcgcaggtc ctcggccgcc catcgctcga ggacccgatg cagtttcggg
     1141 ttgatccgca agaggaagct cttgcgatcg gtcatcccgg atagagagtg ccggcgttga
     1201 ccactggctg cacgcttcgg tcgccggtga ggaccaccat caggttggag accatggcgg
     1261 ccttgcgctc ctcgtcgagt tcgacgatgt gctcggcggc aagctgctct agggcgtcct
     1321 ggaccatggt tacggcaccg cgaacgatga gcttgcgagc cgcgataacg gctccggcct
     1381 gctggcggcg caacatctcg ccggcgatct ccggggcgta ggccaatctg cgcagacggc
     1441 agtcgcggat tgtcaccccg gcctcgtcca ggcggtcctg gagctcactc tgcagcgtcg
     1501 ccgtgacatc gtccgcgttg gcacgaagtg aagggccctc gtcttcgatg tggtcgtagg
     1561 gatactcact cgccacatga cggatcgccg cctccgactg caccttgacg aaatgctcgt
     1621 agtcctcgac gtcgaacgcg gcgcgggcgg tgtctaccac ctgccagttg atgaccgacg
     1681 cgatctccac agggttgccc actgcgtcgt tgaccttgag cacatccgaa tcgaacgtcc
     1741 gaatccgcag tgagacctgc cgacgttgtg tcaaagggtt gacccaacga aacccgttct
     1801 tgcggaccgt ccccgaatag tgaccgaaga gggtcaccac ccgggactga ttcggctggt
     1861 tgatgaacag cccgggcaca atgaaagccc cgatcaggaa caccaggcct gcggccgcga
     1921 tcagccagcc gtcgtcttgt gacagggcca ccacgaaggc gatcaccccg cccaaccaca
     1981 agatcagggc gatagacgcc atcaaccaac ccgagaaacc ctcgaccaac cgctcagcgg
     2041 acatcgattg cctttctgtc atatcacgat gatatcaccg tgatatgaca gtttcaatat
     2101 ccgagctcga tcgatcccga cggatgggag atcgtgagcc tggtgatctc ggagggcccg
     2161 tcgacgacag ccacctcatc ggtgttcgct cccagccagc ctctcaactc ctcggcccgt
     2221 ccgctgagag tcagcttctc catcgggccc tgaccctcga ggaggcgtcc cggatggtcg
     2281 ggcatgtcga tgtcccactg gatgaagaag ggatggcccc ggttcagggt ctgttcgagc
     2341 cccgccaact gccaacgaag ctcaataccg tcgggtgtgg ttctggacat cgcagtcggc
     2401 ttcagctgca agcgtttggt aactgaggca atgtcgcccg tccgcatgca caaacccacc
     2461 ggcagcatcg acttctcttc gatcgagacg ctggcccaca tcccgaacgg gttggctctg
     2521 gctgctcgtc gatcgacgac ggtcaacaat tcgatgtagt tgtcgccgag aggaatgatc
     2581 cggttggcgg tgcccaatcc ggcgtggcgg ccgccggcga ccgaccggag tccatgccgc
     2641 ctcaggaacc agtccgacgc ctgatccaga tcaggtgcac agaagatcac atggtcgatg
     2701 acgaggtcag acttggccaa tgcttcctct cctacctgtt tcgattgttg tcatcacatc
     2761 ggccgtagac tcggccatgt gaaacgtctt tctcccctgc tcatacttgg cgccatcgtc
     2821 gccttcgtct tgatgcgtcg acgtggtggc gacccggtgg agcccgactc cgcctggagc
     2881 ccgattctgc cctcgtgatc gtcggggttc tcatcaccgg agacaccgcg gtccgggccg
     2941 ctcactctct cagcgcccac ccctcagttg atgaagtcgt ggtcgtcggc ccggcgcgaa
     3001 gcaagagctt tcgcgtcgtg ccatcggccg aggagtgtga cttcctgatc gggaccgggc
     3061 ccgatgcgcc ctccctggcc agaggtcacg gcgtcgagtt gatctgggac ggcgaaacac
     3121 tcgaggaagg ggtcgtcgtt gccggttgct ctcccgcagg catggccctc gcgatcgccg
     3181 cccgtgagtc ggacccgcgt ctggtggcgg ttgcccaccc cgatttcgaa gagggctcag
     3241 gccatgtcgt ccggttccct gatccggttg gtgccgcccc tgtcgccgac tccacccttg
     3301 ccaaccgacg tgtggccaca gcgaagtcac caaatcgctt cgctgcgtgt ctggcggtga
     3361 gtgcgggccg cagcgtcacc atcgtcgatg acggtgcctt cctctccggc atcgccctgg
     3421 cagcgggaat cgatgtggcc caggaagagc cgaaattggc cttcgatgac gctctcacct
     3481 acctggagac tgcagccgcc atgggcttgt tgatggcggg ggaccgctaa ttgcggtcgt
     3541 gaggggtcat atccggacct ctcgaaccca attacggacg aggtcaggtg agcggaacgg
     3601 tgactggggc ggtttcctcg gtcgggatgt ccgcctggtc acttggcgcc accgagccgt
     3661 tggtggggcg atagtcggtc gggggatgga ggacctgctg gggcatgacc accgggttca
     3721 gcgtctcttc gaggacgacg acctccaacc ctcctacgac gtctgagaca aggttgtagg
     3781 tgatcgccat cagcaccgtg gcaccggtgg cgagcaccca ccacaccacc gatccgaaga
     3841 caacgacccg gaagaactgc tcgacgttgg cgaaaggctg cgctccttcg tcgatcaatg
     3901 tgatttcgac catgaacgcg gtgagcttgg ccgggatccc ggctcgttcg aagaccgacc
     3961 agaagatgac cgtccccagg accatggcga tggccacgac cgcccagacc accgctgaaa
     4021 ccttcagcgc ggtccaagga tcgatttttc tgatgatgcg tcgaacgcga cggacagttg
     4081 ccataccggc tcctagttgt gacccaagtc tacgagaccg ccacagatga ccaacgacat
     4141 cgagtcggcg tgggttcctt cactcctcga catcgtggac ggtggcgatg gcagagagct
     4201 cgtttcctgc gccgac
//
LOCUS       DBNHXU010000395         6151 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4529313, whole genome shotgun sequence.
ACCESSION   DBNHXU010000395 DBNHXU010000000
VERSION     DBNHXU010000395.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 6151)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 6151)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6151
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4529313"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1316)
                     /gene="glmS"
                     /locus_tag="AC161X_09620"
     CDS             complement(<1..1316)
                     /gene="glmS"
                     /locus_tag="AC161X_09620"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: protein motif:HMM:NF001484.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 -
                     L-glutamine:D-fructose-6-phosphate transaminase
                     (isomerizing) activity [Evidence IEA];
                     GO_process: GO:1901137 - carbohydrate derivative
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HIR4989854.1"
                     /translation="MCGIVGYVGPRPALDVLMPGLARLEYRGYDSAGVALNYGDSITV
                     LKQAGRIADLQNLVDREPPSEAHTGIGHTRWATHGAPNTGNAHPHLDCRREVVVVHNG
                     IIENWMDLKVKLVEGGHVFESDTDTEVVAHMIEDMSDISLADAVRRVMNEADGALALV
                     VMRRSEPDVLVGARRGSPLVVGHGDGENFLASDIPAFLEHTREMVVIEDDRIVEIRPD
                     SIVLSDLEGNQLELDRRTVEWDLEAAEKGGYATFMLKEIFEQPHAIIDTLAGRVIAPG
                     RVVLDELKIDNRRLREVDKVFVVACGTSYHAAMMAKYAIERWTRLPVEIDIASEFRYR
                     DPVLGSNALVIGISQSGETIDTLAAIRYARAQGSTVIGVSNVVESALAREADAVLYTR
                     AGPEIGVAATKTFTTQLVAMQLLGLYLAQVRGTLDPGAIEIQVKEML"
     gene            complement(1605..2957)
                     /gene="glmM"
                     /locus_tag="AC161X_09625"
     CDS             complement(1605..2957)
                     /gene="glmM"
                     /locus_tag="AC161X_09625"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015043821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HIR4989855.1"
                     /translation="MADLRRLLDDGKPIFGTDGVRGAAGSELTPELALAIGRAAGSYL
                     RGGPVVVGRDTRRSGEMLSSALQAGFHAVGIDTVDVGVLPSGGISYLTATSGATMGAV
                     VSASHNPAEDNGIKLLAARGTKLPDEVERELEDRLRSSGFRSALGAAIGTRFPDSQAL
                     ESYVAHLQAVSRYKLAGMRVALDCANGAAFKAGPMLFGALGADVRVFGDAPDGNNINQ
                     GCGATHPEFLAEQAEGRIGLSFDGDADRLIAVDEDGRVANGDVIMAIVARHLKAQGKL
                     TKNLVVATVMSNLGFRKSMAEAEIEIIETKVGDRYVMEALIESEGLLGGEQSGHVIFL
                     DHGQTGDGLLTGVRLLDVLAGTGKELRELRAEAIVEYPQILTSVRVERTVDLESASEY
                     WEQVRAVEEELGGEGRVLVRASGTEPVIRIMVEAPSEDEARHYADRLLEMTESSFGKE
                     "
     gene            complement(2960..3352)
                     /gene="rpsI"
                     /locus_tag="AC161X_09630"
     CDS             complement(2960..3352)
                     /gene="rpsI"
                     /locus_tag="AC161X_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012223971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HIR4989856.1"
                     /translation="MSKPLVQATGRRKSSVARVRLRDGSGQVTLNKRQLEDYFPTMAA
                     RLRVMEPLQITTTQGRYDVDATLEGGGISGQVDALRLGIARALIVLDPELRPMLKKAG
                     MLTRDARVVERKKYGLRKARRAPQYTKR"
     gene            complement(3352..3810)
                     /gene="rplM"
                     /locus_tag="AC161X_09635"
     CDS             complement(3352..3810)
                     /gene="rplM"
                     /locus_tag="AC161X_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017598565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HIR4989857.1"
                     /translation="MTINKTYSPKPGDINRVWHVVDATDLPLGRLASEVATILRGKHK
                     PTYAPHMDGGDFVIVVNAEKVAVTSNKSQTKIYYRHSGFPGGIKAETFENLRERRPEA
                     IIERAVTGMLPKNKLGRQMARKLKVYAGPDHPHVAQSPQALELDIRKVEL"
     gene            3917..5104
                     /gene="ilvA"
                     /locus_tag="AC161X_09640"
     CDS             3917..5104
                     /gene="ilvA"
                     /locus_tag="AC161X_09640"
                     /EC_number="4.3.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR01127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004794 - threonine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine ammonia-lyase"
                     /protein_id="HIR4989858.1"
                     /translation="MITLEDIQEARSRFEGRVTETPLVWSQSFSRLTGRDVYLKAECM
                     QRAGSFKIRGALNVLTRLGGQAVVAASAGNHAQGVALAAAFSGSKCTVFMPEGAAIPK
                     IKATQDYGATVELAGNSLADAIEVATEYAATSGARLVHPYDDPLIIAGQGTLGVEIFE
                     QLPDVGTVVIPTGGGGLLSGSAVGLRSLKPTVKVVGVEIEAAAVYAESRRRGDVVRDF
                     PRGQPTVADGIAVSVPSDLVWEIAQEYVDDFLVVNDAQTTSALTLILERSKLMVEAAG
                     AVGVAALMEKLIPDDAPDPICIVLSGGNIDLMFLGKAVRHGLEASGRFAKFKVLVPDQ
                     PGNLFRILGVIAENGGNLVHVEHHREGFRLPFGTVEIELSVETRDADHAARIASALMA
                     ATS"
     gene            complement(5410..>6151)
                     /gene="truA"
                     /locus_tag="AC161X_09645"
     CDS             complement(5410..>6151)
                     /gene="truA"
                     /locus_tag="AC161X_09645"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HIR4989859.1"
                     /translation="SVLMPVYRLDLAYDGTGFRGYARQDGQRTIQGELEDALRTLLGV
                     SPSTAVAGRTDAGVHARGQVVSFDTDREVNGPQLVRSLNGILGPEMAVSTVARVEDGF
                     SARFSATFRRYRYQLSQAETGDPLARNQVWNVGRRLDLVGMQALAEAIVGAHDFSAFC
                     RSVEGKSNIRLVDEARWDDRDDLLTFWIKANAFCHQMVRSLIGLAYDVGRGFTPADSI
                     SEIIESEDRSRVVTVAPPHGLTLWEVGY"
BASE COUNT         1159 a         1972 c         1870 g         1150 t
ORIGIN      
        1 agcatctcct tgacctggat ctcgatcgcc cccgggtcga gggtgcccct gacctgcgcc
       61 aaatagagcc cgaggagttg cattgcaacc agttgggtgg tgaaggtctt ggtggctgcc
      121 actccgatct caggccctgc cctcgtatag aggacggcat cggcctcacg ggccagagcc
      181 gactcgacga cgttggacac accgatcacc gtcgagccct gggcccgggc gtagcgaatc
      241 gcagcaagag tgtcgatcgt ctcgccggac tgactaatcc cgatgaccaa cgcgttgctg
      301 ccgagtaccg gatcgcggta tcgaaattcg gaggcgatgt cgatctcgac cgggagtcgg
      361 gtccagcgct cgatggcgta cttcgccatc atggccgcgt gataggaggt cccacaggcg
      421 accacgaaga ccttgtccac ttcccgaagc ctgcggttgt cgatcttcag ctcgtcgagc
      481 acgacccgcc ccggagctat tacccgtccg gcgagtgtgt cgatgatggc gtggggctgc
      541 tcgaagatct ccttgagcat gaaggtggcg tatccgccct tttcggcggc ctcgagatcc
      601 cactcgaccg tcctccgatc caactccagt tgattgcctt cgaggtcgga cagcacgatc
      661 gaatcgggcc ggatctcaac gatccgatcg tcctcgatca cgaccatctc ccgggtgtgc
      721 tcgaggaacg ccgggatgtc ggacgccagg aagttctccc catcgccgtg ccccacgacc
      781 agcggtgagc ccctcctggc accgaccagg acatccggct cggagcgacg catcaccacg
      841 agggccaggg ctccgtcggc ctcgttcatg acccgacgaa ccgcatcggc cagagatatg
      901 tcggacatgt cttcgatcat gtgggcgacg acttcggtat cggtgtcact ctcgaagacg
      961 tggccgccct cgaccaactt caccttcagg tccatccagt tctcgatgat cccgttgtgc
     1021 accacaacca cctcacgtcg acagtcgaga tggggatggg cgtttcccgt gttgggagcg
     1081 ccgtgggtgg cccaccttgt gtgcccgatc ccggtgtggg cctcggaggg tggctcccga
     1141 tcaacgagat tctgaagatc cgcaatcctt ccggcctgct tgaggacggt gatcgagtct
     1201 ccgtagttga gggcgacgcc ggccgagtcg taaccccgat actcgagccg ggcgagaccg
     1261 ggcatcaaga catcgagagc gggccgtggc cccacatatc cgacaattcc gcacataaca
     1321 gttccttcat tcgtcgaaat tgcgaacatc agggatcaaa tacggaccct cacgtccaca
     1381 atttcaggtg tggtggatgg cgtgcggggg agccgatgac cggcgctcat gagtgagtct
     1441 ctgttccgag atctctcccg ggttttctac tcatccctta cgacctgagc tggccgggaa
     1501 ggcatccgcc gaatctttcg atgaccttcc tcctcgtcac ctcgcagact gcgaggcctg
     1561 gcgcttgtct gttcgcacgc tgttgaatgt tgttttgggt ctgcctactc ctttccgaac
     1621 gaactctcgg tcatttccag tagacggtca gcgtaatgcc tcgcttcatc ttcggaaggg
     1681 gcttccacca ttatccggat cacgggctcg gtccccgaag ccctgaccag cacccggccc
     1741 tctcccccca actcctcttc gacagccctg acctgctccc aatactccga tgcggattcg
     1801 agatccaccg tccgctccac cctgacgctg gtcaggatct gggggtattc aacgatcgct
     1861 tctgcccgga gctcgcgcaa ctccttcccg gtgccagcca ggacatcgag cagtcggacc
     1921 ccggtgagca gcccgtcacc ggtctgaccg tggtccaaga agatcacgtg ccctgattgc
     1981 tcaccgccca agaggccttc gctctcgatc aaggcctcca tgacgtagcg gtcgccaacc
     2041 ttggtctcga tgatctcgat ctcggcctca gccatcgact tccgaaaccc gagattcgac
     2101 atcaccgtgg ccacgacgag attcttggtg agcttgccct gtgccttcag gtgccgggcg
     2161 acgatcgcca tgatgacgtc tccgttggcg actcgaccgt cctcatcgac cgcgatcaaa
     2221 cggtcggcgt caccatcaaa agagagaccg atccgacctt cggcctgctc ggccaagaac
     2281 tccggatgag tggcaccgca accctgattg atgttgttcc cgtctggtgc gtcgccgaag
     2341 acacggacat ccgcacccaa ggcgccaaac aacatcgggc ccgccttgaa cgcggcccca
     2401 ttggcacagt cgagggccac cctcatccct gccaacttgt accgggagac ggcttggaga
     2461 tgagcgacat atgactcgag ggcctgtgag tcgggaaaac gggtgccgat ggcagcgccc
     2521 aaggcagagc gaaagcccga ggatcgcaac cggtcttcga gctctcgctc cacctcgtcg
     2581 ggcaacttgg tgccccgggc cgcgagaagc ttgatcccgt tgtcttcggc tgggttgtgg
     2641 gaagcggata cgaccgcccc catcgttgcc cccgacgttg ccgtgaggta actgatcccg
     2701 ccgctgggga gcacaccgac atcaacggtg tcgatgccta cggcgtggaa gcctgcctgc
     2761 agcgccgatg aaagcatctc gcctgatcga cgggtgtctc tgccaacgac aacgggccca
     2821 ccccggagat aggaccccgc ggcacggcca atggcgagag ccaactcagg tgtcagttcg
     2881 gagccagcgg caccacggac accatcggtt ccgaagatgg gcttcccatc gtcgagcaga
     2941 cgacggagat ccgccaactt cagcgcttcg tgtactgagg ggcccggcgc gctttgcgga
     3001 ggccgtactt cttgcgctcg accacccgtg catcacgggt gagcatgccg gccttcttca
     3061 gcatcggcct gagctcgggg tcgagaacga tcagagcgcg ggcaatgccc aagcgaagtg
     3121 catccacctg acctgagatc ccgccgccct cgagggtggc gtcgacgtcg tagcggcctt
     3181 gagtcgtcgt gatctgcagt ggctccatca cccgcagccg ggcggccatg gtcgggaagt
     3241 agtcctcgag ttgacgcttg ttgagggtca cctggccgga tccatcgcgg agtcgcactc
     3301 tggcgaccga agacttgcga cgaccggtcg cctggacgag gggtttggac atcagagttc
     3361 cacctttcgg atgtccaact ccagggcctg cgggctctgg gcgacatggg gatgatcagg
     3421 cccggcatag accttgagct tgcgcgccat ctggcgtccg agcttgttct tgggaagcat
     3481 tccggtgacc gcccgctcga taatggcttc cggccggcgc tcacggagat tctcgaacgt
     3541 ctccgccttg atgccgcccg ggaaacccga gtgccggtag tagatcttcg tctggctctt
     3601 gttggaggtc acagcgacct tctcggcgtt gaccacgatc acgaagtcgc caccgtccat
     3661 atgcggcgca tacgtcggct tgtgctttcc ccgaagaatg gtggcgacct cagaggcgag
     3721 gcggccgaga ggaagatcgg tggcatcgac gacatgccaa acacgattga tgtcaccagg
     3781 cttgggcgaa tatgtcttgt ttatggtcat ctgattggcg gcccggcagg tcgaaaaata
     3841 ccgggacgtt gggaaacggc tcccgcttgg ggagcgggca tatgctacgc cggagccaga
     3901 gactaggcca atcgcgatga tcacgctcga ggacatacaa gaagcccgtt ccaggttcga
     3961 gggacgggtg accgagaccc ccttggtgtg gtcgcaatcc ttcagtcgtc tcaccgggcg
     4021 cgatgtctat ctcaaagccg agtgcatgca gcgtgccggt tcgttcaaga tccgcggcgc
     4081 actcaacgtc ttgacgaggc tcggcgggca agccgtggtt gcggcctccg ccggcaacca
     4141 cgcccagggt gtcgccctgg ctgctgcctt ttccggctcg aaatgcacgg tcttcatgcc
     4201 cgaaggggcg gccataccca agatcaaagc cacccaggat tatggggcga cggtcgagct
     4261 ggcagggaat tctcttgcag atgccatcga ggtcgccacg gaatacgcgg cgacttctgg
     4321 cgcccgcctg gtgcacccat acgacgaccc gctgatcatc gccggccaag ggactttggg
     4381 cgtcgagatc ttcgaacagc tgcccgacgt gggcacggtc gtgatcccca ccggcggcgg
     4441 cgggctcttg tcaggcagcg cggtcggact gcgatccctc aagccgacgg tcaaggttgt
     4501 cggagtcgag atcgaggcgg cagccgttta cgcagagagt cgtcgcaggg gcgatgtagt
     4561 gcgtgacttc cccagaggac agccgactgt tgcagacgga atcgcggtat cggtcccctc
     4621 ggacctggtt tgggagatag ctcaggagta cgtcgacgac ttcctggtgg tgaacgacgc
     4681 tcagacaacc tcggccctga cactcatcct ggagagatcc aagctcatgg tggaggcggc
     4741 cggggcggtg ggggtggcgg cgttgatgga gaagctgata cccgacgatg cccccgaccc
     4801 catctgcatc gtgctctccg gcggcaacat cgacctgatg ttcctgggca aggcggtccg
     4861 tcacggcctg gaagcctcgg gcaggttcgc caagttcaag gttctggtgc ccgatcagcc
     4921 gggcaacctg ttccggatcc ttggcgtgat cgccgagaac ggcggcaatc tggttcatgt
     4981 cgaacatcac cgggagggat tccgcctccc attcggcacg gtcgagatcg agctctcggt
     5041 cgagaccagg gacgccgacc acgctgcccg catcgccagc gcattgatgg ctgccaccag
     5101 ctgaaatagg cataggggtc ttcccccagg ggagggttcc ccatatgcac tccggtcctg
     5161 acgatgtcct attgcacata cggccgccaa cggccgatga gcaggaggac caaagtgcta
     5221 cagagaatcg ttatgcgatc aaccggaaaa ccacaaccat cgaagttcgc cgtggaacgt
     5281 gcccggctcc gtcaaccgtc gatcgacgcc gagcccccgc gcaacccggt gttcgacgcc
     5341 atcggcaggg ggctcatgac cctcgggcag agagtctcgt ctcgcgggtt cgaccctggc
     5401 cggatctgat caataaccga cctcccacag ggtcagaccg tgaggcgggg ccacggtgac
     5461 cacccggctc cgatcttcgg actcgatgat ctcggagatc gaatccgctg gggtgaatcc
     5521 gcggccaacg tcgtaggcga ggccaatcag ggatcgaacc atctgatggc agaaggcgtt
     5581 ggccttgatc caaaaggtca gcaaatcgtc tctgtcatcc catcgcgcct cgtcaacgag
     5641 acggatgttg ctcttcccct cgaccgatcg gcagaaggcg ctgaagtcgt gcgcacccac
     5701 gatggcctcg gccaaggcct gcatcccgac cagatccagc cggcgtccca cattccagac
     5761 ctgatttcgg gcgagaggat cgccggtttc cgcctgggac aactgatatc gataacgacg
     5821 gaacgtcgcc gagaatcggg cgctgaaacc gtcttcaacc cgggccaccg tcgaaaccgc
     5881 catctccgga ccaaggatcc cattcaatga gcggacgagt tgagggccgt tgacctcacg
     5941 gtcggtgtcg aaggagacca cctggccacg ggcatggaca ccggcgtccg ttcggcccgc
     6001 caccgccgtc gaaggcgata ccccgaggag tgtgcgtaga gcatcctcga gctcaccctg
     6061 aatcgtgcgc tggccgtcct gtcgggcata tccgcggaac ccggtgccgt cataggccag
     6121 gtcgagccga tagacaggca tcagaacaga g
//
LOCUS       DBNHXU010000396         1693 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3537105, whole genome shotgun sequence.
ACCESSION   DBNHXU010000396 DBNHXU010000000
VERSION     DBNHXU010000396.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1693)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1693)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1693
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3537105"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..972
                     /locus_tag="AC161X_09650"
     CDS             <1..972
                     /locus_tag="AC161X_09650"
                     /inference="COORDINATES: protein motif:HMM:NF022077.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CpaE/TadZ family protein"
                     /protein_id="HIR4989860.1"
                     /translation="DPVLVLMMVAETVTADLLRYAMRAGVSDVIESPLDETKIEAAIE
                     QFAHDVLKRRNVAARSGREVERTERGQLITVTSAKGGSGKTVMATNVALLLNRLADKK
                     VVLVDADLQFGDVCLLLQLEPRFTMVNAAHELHQLDSEMLDSLLTEHPSGLRVLAAPL
                     EPAFADDITTAGLMQMLDVLQETYDYVVVDTASMLDELILSLIEKADQVLMIVDMDLP
                     SVKNAKLALETLRLLKFSTGNVKLVMNRSNSKARLDNKEIEGALKMEIAAAVPSDAAV
                     PNSVNEGRPVVETDPKGKVAKGFESVAALIAGKIPESSQKSGLFGRK"
     gene            990..>1693
                     /locus_tag="AC161X_09655"
     CDS             990..>1693
                     /locus_tag="AC161X_09655"
                     /inference="COORDINATES: protein motif:HMM:NF012652.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HIR4989861.1"
                     /translation="MPSLSERVAAARQSETADVGVNRSDAMSELRGRLHFKVVEELGP
                     TLYDRQMSDAELRLRVLEMLEWAVDQEQGLPLTSADRRALLNEIASDVLGYGPIDPLL
                     NDPDVTEVMANGPYDIYYEKLGKITLSDVKFVDEVHLRRIIDKIVGQIGRRVDEATPM
                     VDARLPDGSRVNAVVHPLAIGGPFLTIRKFAVDPYQEADLINFGTITQRVADFARACV
                     RGRLNILISGGTGSGKT"
BASE COUNT          324 a          536 c          508 g          325 t
ORIGIN      
        1 gatccggttc tcgttctgat gatggtggcc gagacggtca ccgccgacct cctcaggtac
       61 gccatgcgtg ccggggtgtc ggacgtcatc gagtccccgt tggacgagac caagatcgag
      121 gctgcaatcg agcagttcgc tcacgatgtc ctgaagcggc gcaacgtcgc cgcccgttcg
      181 ggacgtgagg ttgagcgcac cgagcggggc cagctgatca cggtcacctc ggccaagggt
      241 ggaagcggca agaccgtgat ggccaccaac gttgccctcc ttctcaaccg cctggccgac
      301 aagaaggttg tgctggtcga tgccgatctc cagttcggcg acgtctgcct gctccttcag
      361 ctcgagccgc gtttcaccat ggtgaacgcc gcccacgagt tgcatcagct cgattccgag
      421 atgctcgatt cgttgctgac cgagcatccg agcgggcttc gggtcctggc tgcgccgttg
      481 gagccggcat ttgccgacga catcacaacg gccggcctga tgcagatgct cgatgtcctt
      541 caggaaacct acgactacgt cgttgtggac accgcctcga tgctcgacga gttgatcctg
      601 tcactcatcg agaaggcgga tcaggtcctg atgatcgtgg acatggacct gccttcggtc
      661 aagaacgcca agctcgccct ggagaccctc cgcctcctca agttctccac gggcaacgtg
      721 aaactcgtca tgaaccgctc caactccaag gcccgcctcg ataacaaaga aatcgaaggt
      781 gccctcaaga tggagatcgc agctgccgtt ccctctgatg ccgccgttcc caactcggtc
      841 aacgaggggc gcccggtggt ggagaccgac cccaagggga aggtcgccaa gggcttcgag
      901 agtgtcgctg ccttgattgc ggggaagatc cccgaaagct cgcaaaagag cgggctcttc
      961 gggcgtaagt agtgggaagg ttgtgaacgg tgccatcttt atctgaaaga gttgcagcag
     1021 ccaggcagag cgagaccgcc gacgtcggcg tgaaccgcag cgacgcgatg agcgagcttc
     1081 ggggtcgtct ccacttcaag gttgtcgaag agctcggtcc gactctctat gaccgccaga
     1141 tgtccgacgc cgagttgcgc ctccgcgtgc tcgagatgtt ggaatgggcc gtcgaccagg
     1201 agcaggggct tcccctcacc tcggccgacc gtcgtgccct tctcaacgag atcgcctccg
     1261 acgtcctcgg ttatgggccc attgacccgc tgctcaacga cccggacgtc acagaagtca
     1321 tggccaacgg tccgtacgac atctactacg agaaactggg aaagatcacc ctgtctgacg
     1381 tcaagttcgt cgacgaggtc catctccgca ggatcatcga caagatcgtc ggccagatcg
     1441 gtcgtcgcgt cgacgaagcc accccgatgg tggatgctcg tctccccgac ggctcacgtg
     1501 tcaacgcggt ggttcacccg ttggcgatcg gcgggccgtt cctgacgatt cgaaagtttg
     1561 ccgtcgaccc ctaccaggag gccgacctca tcaacttcgg caccatcacc cagcgtgtgg
     1621 ccgactttgc ccgggcctgt gtccgtggac ggctcaacat cctgatctca ggtggtaccg
     1681 gttccggtaa gac
//
LOCUS       DBNHXU010000397        14740 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4531090, whole genome shotgun sequence.
ACCESSION   DBNHXU010000397 DBNHXU010000000
VERSION     DBNHXU010000397.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 14740)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 14740)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14740
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4531090"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..951)
                     /gene="fabD"
                     /locus_tag="AC161X_09660"
     CDS             complement(16..951)
                     /gene="fabD"
                     /locus_tag="AC161X_09660"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: protein motif:HMM:TIGR00128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HIR4989862.1"
                     /translation="MSVGILFPGQGSQHVGMGRDLFEARPDLLGDLADHILGFSLRGI
                     CLDGPEDDLTRTEHAQPALFALSFALWDTLAHEAGFTPAGCAGHSLGEYTALTAAGVF
                     DFDSALSVVARRGQAMADAADLEPSGMAALLGSEEDEAEAICAGRRETGGRLEVANIN
                     APGQVVVAGGMADIEWLVENARDLGVRRVIPLKVSGAFHSTFMTPAAETLAIALAGVE
                     LGDPRFPVWANATGRPHMPDEIRESLTRQVVSPVRFAESLTDMAGAGIDTFVHVGPGD
                     VTAGLARRTLPDANVLVVSGIEDIGAAAEAIGTMP"
     gene            complement(983..2008)
                     /locus_tag="AC161X_09665"
     CDS             complement(983..2008)
                     /locus_tag="AC161X_09665"
                     /inference="COORDINATES: protein motif:HMM:NF041359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004793 - threonine aldolase activity
                     [Evidence IEA];
                     GO_process: GO:0006567 - L-threonine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine aldolase family protein"
                     /protein_id="HIR4989863.1"
                     /translation="MAFADGIADFRSDTVTRPTPEMLAAMVSAPLGDDVYHDDPTVNL
                     LEEESAAVTGKESAIFVPTGTMGNQLAIMAQTRPGEEILAHEASHVRSIEGGAPQALS
                     GVGFRTVSGDGGRIEPEDVERAMELSGFFPRIGLMVWENTHNLSGGRVIPIDLMEKTS
                     QVAKAHSLRIHIDGARIFNAVTASGVGADRYAAVADTIQFCFSKGLGAPVGSILCGPT
                     DLIDEVRYLRKRLGGGMRQAGVLAAAARIALAARDRLVEDHLLARDIAEGLADRFPGS
                     VGADVTETNMVRVEFAALGMEWGDVRSRLEAAGIKSSAPIAGAWRIVAHRDVDARDVE
                     RLFGALQ"
     gene            complement(2037..2468)
                     /locus_tag="AC161X_09670"
     CDS             complement(2037..2468)
                     /locus_tag="AC161X_09670"
                     /inference="COORDINATES: protein motif:HMM:NF018508.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YkvA family protein"
                     /protein_id="HIR4989864.1"
                     /translation="MTQEPISIRPDEVLPPPYGSGVQVRQLAKDAVLLVPNFAKLVAR
                     LLMDPRVPRRSKIALGMAAAYVASPIDLLPEFIPVIGWLDDLLILTYVLDRVIDRAGK
                     EVVEELWDGPGDLLGLIREVLGLASNVIPKKLRVVLDRLHG"
     gene            complement(2465..2650)
                     /locus_tag="AC161X_09675"
     CDS             complement(2465..2650)
                     /locus_tag="AC161X_09675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989865.1"
                     /translation="MKDQSETVQALARAGRRVVYHLMQAVVEGLKAVEAVIEEVGSIR
                     DNDDNKAETTRTRIEIE"
     gene            complement(2647..3825)
                     /locus_tag="AC161X_09680"
     CDS             complement(2647..3825)
                     /locus_tag="AC161X_09680"
                     /inference="COORDINATES: protein motif:HMM:NF014433.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ArsA family ATPase"
                     /protein_id="HIR4989866.1"
                     /translation="MRIVLVTGKGGVGKTTVAASTALASADAGYRTLVTSTDPAHSLA
                     DALEVPLGSDPTKVVSGLDGQQIDTQRQLDKYWGSIRRQLMDVLDWGGVAGIEAEEFL
                     VFPGMDELFALLDVNRHARSGVYDVIVVDCAPTAETLRLLSLPEVLSWYFEKVMPTER
                     RLMRAARPILSRLTDLPLPDDEVFIAAHSVFESIEDVKELMSSPEVTSARLVVNPEKM
                     VIDEARRTYSYLGLFGYGVDGVVVNRVLPDLVADPYFERWRAIQKGHLDTIDDAFPEI
                     PRLWLRLFDDEMVGVDRLRFMATELYGDKDPIEDFSATAPFRIVEVNGSVSMEVDLPF
                     FEKSDLDVYRSGHELYIQVGPYRRSFILPDALHRREVTRARLDEGTLSVAFSDPDARS
                     "
     gene            complement(3827..4264)
                     /locus_tag="AC161X_09685"
     CDS             complement(3827..4264)
                     /locus_tag="AC161X_09685"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4989867.1"
                     /translation="MAEGTFQTLEIDAAPEVLYDAAADIGSYPEWATGVKEVEVLESG
                     PDGRVDRARFVIEVFVKEIEYVLRYEHDRPNRLSWVAEESDDLQMLEGSYTFTANDGV
                     TEVQYGLKVGLKFTMPGFLMRQAEKQIVTTALRGLRKRVTGAS"
     gene            complement(4311..5087)
                     /locus_tag="AC161X_09690"
     CDS             complement(4311..5087)
                     /locus_tag="AC161X_09690"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012376.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HIR4989868.1"
                     /translation="MILVISDVHFALDALHRVVGMGAPVLILGDLVNLTDYRTGEGAV
                     ADVLGLEFARATAEARAAGDYQGMRDLWADSAEDRYDEVREKIGAAIDAQYQGVSGAL
                     EGGSGYVIHGNVDRPRSLRASLPSGFEYVHGQVVELEGLTFGFVGGGAPTPLQAEGEI
                     SDDEMTSLLEQLGPVDVLCTHVPAAVHSLRTDVITGRAERGSTPILEYLVREQPRLHL
                     FGDVHQPQATTWRVGSTLCRNVGYFRATGRPLRIEPSRDD"
     gene            complement(5084..5377)
                     /locus_tag="AC161X_09695"
     CDS             complement(5084..5377)
                     /locus_tag="AC161X_09695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989869.1"
                     /translation="MVKVYAVALAVGVIGLLVVILGGAFAENLNREQADPGKRLGKTG
                     RAAIGGLVGFGMAGLSAEFAPLDFDWAIALLLALTGGIAGAIWGYYGAGVADN"
     gene            complement(5435..5596)
                     /locus_tag="AC161X_09700"
     CDS             complement(5435..5596)
                     /locus_tag="AC161X_09700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018685730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989870.1"
                     /translation="MRDTSACDDCVVTFLLDGGPLELDESESEALRNLAGEGLVPRLR
                     LVPVERKTG"
     gene            complement(5671..6579)
                     /locus_tag="AC161X_09705"
     CDS             complement(5671..6579)
                     /locus_tag="AC161X_09705"
                     /inference="COORDINATES: protein motif:HMM:NF015995.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HIR4989871.1"
                     /translation="MRKAFRFRAVGAILSLALLATACGSGDGATEGEPIKVASFNFNE
                     SVIVAEIYAQGLEANGYAIERRMNLGAREVVKPALESGEIDLVPEYTGTVAGFLEVEQ
                     SADPQTTWEAARDAYSELGVTLLAFSPAQDKNAYVVTAETASEFGLENVSDLQPVAGE
                     MIFGGPPECPARPLCLQGLEDVYGLEFSEFKALDVAGPITIAALEGGEIDVALLFSTQ
                     GIIAANGWVSLADDKGLNPAENLVPAIRTEVVEAYGDELVALLDSYSEALTTEDLTEL
                     NKRADLDQEDPAVLAGEWLAEHGFVE"
     gene            complement(6664..7350)
                     /locus_tag="AC161X_09710"
     CDS             complement(6664..7350)
                     /locus_tag="AC161X_09710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989872.1"
                     /translation="MTVWESMREFLSEPSTWSGPDSIWVRLGEHIAVSVAAVVIAGIL
                     VLPLAVWLGHIRKGSAIATAVVNIGRAVPSFGVLAIAFLIFVQIGAGIGEPWPVLVAL
                     IALAAPPMFTNTIAGIQTVEPTTLESARGMGLTEREVLTGVELPLSMPLIMEGVRIAF
                     VQVIATATLGALVAWGGLGRFIVDGFAQQDVGELIVGAILVGMLAIAAELGLSATQRA
                     TTPRGLRVGR"
     gene            complement(7347..7955)
                     /locus_tag="AC161X_09715"
     CDS             complement(7347..7955)
                     /locus_tag="AC161X_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020668630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989873.1"
                     /translation="MGEILDRTWQHLSLTAVSVALGLVISLGLTAVVLRWRRTYTPIT
                     WVTGLLYTIPSLALFALLVPIVGLNATNAVIALTSYTLLIIIRNMVAGINGVPEPVLE
                     AADGMGYTRRARFWRMEIPLALPVIIAGLRIATVTTVGLVTVTAVLGLGGYGYFILRG
                     LNTFFWTQILVGVTLSVVLATILDLAFIWLQSALSPWARSPK"
     gene            complement(8003..9106)
                     /locus_tag="AC161X_09720"
     CDS             complement(8003..9106)
                     /locus_tag="AC161X_09720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010639369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989874.1"
                     /translation="MTDTMIRLEKVAKVFPGASRPAVDDLDLDVGKGEFVTLVGPSGC
                     GKTTTLKMINRIIEPSSGRILIDGADVVAVPPHELRRSIGYVIQQVGLFPHRTIAQNI
                     ATVPALMGWDAGRIKERIEQLAALVGLDPEMLDRYPAALSGGQQQRVGVARALAADPP
                     LLLMDEPFGAVDPIVRKRLQHEFQALQQRLGKTVVFVTHDVDEAILLGDRMAILNVGG
                     VLEQFDEPATILANPANAFVEDFLGGERGLKRLALLPVGEAQGIRWGPVVDVSVSPEE
                     AKRVMEEESTDWIGLLEEGRLLGWVDGSAVGTGPLRDLVPRPFLVTLGSDASLRSALD
                     AVVTSHARVAMVVDQGLYRGMLDLEGISEEITE"
     gene            complement(9155..10066)
                     /locus_tag="AC161X_09725"
     CDS             complement(9155..10066)
                     /locus_tag="AC161X_09725"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HIR4989875.1"
                     /translation="MTLRTYLWGAFWTLVGLALVFHVVGGWYFSGELIDDGFEIQAEP
                     LVVPSGSYQLEEVAYASPLGSMAAWYLPAAGTTWVIHVHGKGTTPAEAEPMFAPLQEA
                     GYPQLAITYRNDDGQPVDPSGYYQYGVTEWEDVAGAVTYALDNGAERVILNGYSTGGA
                     HVLSFMYHQSLDTVGGVLLDSPNIDLAETVDYRASQRELPILPFNVPTSLSTVAQFFT
                     SLRIEFNWQSVDYVEKAADSLRQPVLVHHGTADLTVPISESIELQETQPDLVQLIQVE
                     GADHVGSYDVDPQKYISEVLGFIQEVG"
     gene            complement(10088..10993)
                     /locus_tag="AC161X_09730"
     CDS             complement(10088..10993)
                     /locus_tag="AC161X_09730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989876.1"
                     /translation="MTVGGQAGGGFSVVWTRGKGCSELADLLAGAQSNRITGDLVEAC
                     ISYRADLLVSRKLPGSFDLVNVAVPVAFVPENVTGVVATVAGGPHSILAAQTAALVGA
                     SLGVEAEMVSASGPGDDPSAAQLTLDRIAAVVPRLKGRVVGVEGISSLAQDLDEGSLL
                     VLGAPGGSWLQRSMLGPGARLRSKAQAGAVMVRSAPERVFRWMGDPVFVGPLRQANDT
                     LRIHTEKVLAVADQGVLIGLVRRDRLEQSGDTVVGSIMEEAVSAKVDETLAEARELEP
                     TFGEDPIPVTDHEEHLVGGLSLPAA"
     gene            11110..11706
                     /locus_tag="AC161X_09735"
     CDS             11110..11706
                     /locus_tag="AC161X_09735"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014982938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HIR4989877.1"
                     /translation="MEFKEVIAARRMVRNYLPDPVARESLDRIVDSGLRAPSAGFSQG
                     LGFVVVTEPEMRQAIAELADEAEYVASGFDPWISRAPAHIIITVSEKVYRDRYREPDK
                     LGPDGAEIEWPVPFWWVDAGAALMAVLLAAVDEGLAAGFLGVHSLPDLATLLRLPPDH
                     LPIGVVTIGAPAPDRRSSSLTRGWKRRESVVHSETWGQ"
     gene            11703..12479
                     /locus_tag="AC161X_09740"
     CDS             11703..12479
                     /locus_tag="AC161X_09740"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012797.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrmH family RNA methyltransferase"
                     /protein_id="HIR4989878.1"
                     /translation="MTDLAITSHANPRIKALVGLRDRGARDRQRLFVVEGPRLVERAL
                     AAGHRPVEVFYDPNRFDPGPLTGPTVFPCSAEVLSRASYRASDEGVIAVVPQFDLDLS
                     GLALPEVPLILVAEGIEKPGNLGAILRTADAVGADAVVAVDPEIDPFNPNVVRSSTGA
                     LFTVPIAVSGLENALHWLHEAGIRLIGADPSADHDLWSIDFTQPCALMVGSEHGGLTD
                     RVRQAADGLASIPMRGATDSLNASVTIALLAYEALRQRRA"
     gene            12529..14157
                     /locus_tag="AC161X_09745"
     CDS             12529..14157
                     /locus_tag="AC161X_09745"
                     /inference="COORDINATES: protein motif:HMM:NF005907.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003724 - RNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000027 - ribosomal large subunit assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEDD exonuclease domain-containing protein"
                     /protein_id="HIR4989879.1"
                     /translation="MSALLQPSFDELSTPLYAVTFCVLDLETTGGSAKDCEITEIGAV
                     KYRGGELLGSFQTLVDPGLPIPPSITILTGISEAMIFDAPKIESAFPTFLEFIGDAVV
                     VGHNVRFDMSFLQAAATRLGFERMANDTVDTAALARRLVRDEVRNLRLRTLAAHFRSP
                     VTPIHRALDDAQATAHVLHALLERVGNLGVTNLDDLLQLPKARGSAFYSKISLSEGLP
                     RRPGVYIFRDRHGTPIYVGKATNLRARVRQYFYGDERRSIGNLMRELVSIDHVLCETP
                     LEAEVTELRLIHAHRPRHNRRSRPPKTSHWVKLTREPFPRLSVVRTLKEDGLAHLGPF
                     RSKQAADLVVTALWDATEIRRCRSSASERSGPCAAAQLGVAACPCDGSLLPGDYRPIV
                     ERLIEGLDRNPALLLTPLAEKMAELADQHRYEEAGWLRDRHHALSRAIERRQVWSALS
                     AFGLCEVESRDGCRVLIDHGKLVSTWRLDRTPPLRPAPEVGEHGNRQVPESVEIAEEA
                     DLIWKWLDTTEIRLVDATGLLALPVHQVPRLRAS"
     gene            14199..>14740
                     /locus_tag="AC161X_09750"
     CDS             14199..>14740
                     /locus_tag="AC161X_09750"
                     /EC_number="7.5.2.-"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HIR4989880.1"
                     /translation="MGRLVDLNQIAVSLGGLPVLRDIELAIDAGETLGVSGPNGAGKT
                     TLLRVLATLLAPITGSGTVLGAELGTAAVYPVRRSIGLLSHRPALIPELTLAENLRHA
                     ANLSGIEFSKAETALRVVGLDEVSSRTAAASSFGMLRRTEVARLLITSPTILLLDEPF
                     TGLDVEAQELIGALVTRTTG"
BASE COUNT         2747 a         4881 c         4493 g         2619 t
ORIGIN      
        1 ggcatctcct caaggtcagg gcatggtacc gatggcttcg gccgccgccc cgatgtcttc
       61 gattccagag acaaccagca cattcgcatc tggcagggtg cggcgggcaa gcccggcggt
      121 cacgtcaccg ggccccacat ggacaaaggt atcgatgccg gcccctgcca tatcggtcag
      181 gctttcggca aagcgcacag gtgacaccac ctggcgggtc aatgactcgc gtatctcatc
      241 gggcatgtgt ggccgccccg tcgcattggc ccacaccggg aatctcggat caccgagctc
      301 gactccggcc agggcgatgg caagggtctc ggcagccgga gtcatgaagg tcgagtgaaa
      361 tgcgccggac accttgagtg ggatcacgcg cctgacaccg agatcccgag cgttctcgac
      421 gagccattcg atgtctgcca tcccgccggc tacaaccacc tgacccgggg cattgatgtt
      481 ggccacctcg agtcgccctc ccgtctccct cctccctgca caaatcgcct ctgcctcgtc
      541 ctcctccgat ccgagaaggg ccgccatccc ggagggctcc agatcggcgg cgtccgccat
      601 tgcctgaccg cggcgagcaa cgaccgacag agcggagtca aagtcgaaca cccctgcggc
      661 cgtcagggcg gtgtattccc ctagcgagtg cccagcacag cctgctgggg tgaacccggc
      721 ctcgtgcgca agagtgtccc aaagggcgaa ggagagggcg aacagggccg gctgagcgtg
      781 ctcggttcgg gtcaggtcat cctcggggcc atccaggcag attccccgca atgagaaccc
      841 gagtatgtgg tcagctaggt caccgaggag gtcgggcctg gcctcgaaga ggtccctccc
      901 catcccgacg tgctgactcc cctgaccggg aaagaggatt ccgacactca cttgctatcc
      961 gactcctgag ctcgaggctc ggttactgga gggcgccgaa gaggcgttcg acgtcacggg
     1021 cgtccacgtc gcggtgagcg acgatcctcc aagcgcctgc gattggcgcg ctggatttga
     1081 ttccggcagc ttcgagcctg gatctgacat cgccccattc catccccagg gcagcgaatt
     1141 cgactcgcac catgttggtc tcggtgacat cggcgcctac cgaaccgggg aacctgtcgg
     1201 ccaacccctc ggcaatgtcc cgggccagca gatgatcttc gaccagccgg tctctcgcgg
     1261 cgagggcgat tcgggccgcg gcggccaaca cccccgcctg acgcatgcca ccgccgaggc
     1321 gcttgcggag gtacctcact tcatcgatga gatccgtcgg accgcagagg atcgagccca
     1381 ccggagcccc gagacccttg gagaagcaga actggatggt gtcggcgacc gccgcataac
     1441 gatcggcacc cacacctgac gccgtgactg cgttgaagat ccgggcgcca tcgatatgga
     1501 ttctcaagga atgagccttc gccacctggg acgttttctc catgagatcg atggggatca
     1561 ctcgtccccc ggagaggttg tgggtgttct cccagaccat cagcccgatt cgcggaaaga
     1621 agccactcag ttccatcgcc cgctcgacat cctcgggctc gatccgcccc ccatcaccgg
     1681 agacggtgcg gaagccgaca ccggaaagcg cctgcggggc gccgccctcg atgctgcgaa
     1741 cgtggcttgc ctcgtgggcc aggatctcct caccgggacg ggtctgggcc atgatcgcca
     1801 gttgattgcc catggtccct gttgggacga atatcgccga ctccttgccc gtaaccgccg
     1861 ccgactcctc ttcgagcagg ttcaccgttg gatcgtcgtg atagacgtcg tctcccaggg
     1921 gcgccgagac cattgccgcc agcatctcgg gcgtcgggcg ggtgaccgtg tctgatcgaa
     1981 agtcggcgat gccgtcggcg aatgccatgc tcgaaggtta ggacgagccc aggatgtcag
     2041 ccgtggagac ggtcgaggac gacccgcaac ttcttgggga tgacgttgct ggcgagcccc
     2101 aagacctccc ggatcagccc caacaggtca ccggggccat cccacaactc ctcgacgact
     2161 tccttccctg ctcggtcaat gacacgatcg aggacatagg tcaggatcag gaggtcatcg
     2221 agccatccga tcaccgggat gaactcagga agcaagtcga tggggctggc cacataggca
     2281 gcggccatgc cgagggcgat cttggagcgc cgcgggaccc gagggtccat gagaagccgt
     2341 gccaccagct tggcaaagtt cggcactaac aagacggcgt ccttggcaag ttgccgcacc
     2401 tgaaccccgc tcccgtaggg agggggcagc acctcatcgg ggcggatgct gattggctct
     2461 tgggtcattc gatttcgatc ctggtcctgg tcgtttcagc cttgttgtca tcattgtcgc
     2521 ggatgctgcc tacttcttcg attacggcct ccaccgcctt tagcccttcc accactgcct
     2581 gcatcaggtg gtacacgaca cggcggcccg ctctggccag cgcctgcacc gtctctgatt
     2641 gatctttcat gatctcgcat ccggatcaga gaaggccact gacaatgttc cctcgtcgag
     2701 ccgggcccgg gtgacctccc gccgatggag ggcgtcggga aggatgaagg agcggcggta
     2761 gggcccaacc tggatgtaga gctcatggcc gctgcggtag acgtcgaggt ccgacttctc
     2821 gaagaatgga aggtccactt ccatggaaac cgagccattc acctcgacga tccggaacgg
     2881 ggcggttgca ctgaagtcct caatcgggtc tttatccccg tacaactccg tggccatgaa
     2941 ccgcagtcga tcgacgccaa ccatctcgtc gtcgaacagc cgcagccaga gccgggggat
     3001 ctcgggaaag gcgtcatcga tggtgtcgag atggcctttc tggatcgccc gccagcgctc
     3061 gaagtagggg tcggcgacca gatcagggag gacccgattc acgactaccc cgtcgacgcc
     3121 gtacccgaac aggcccaggt agctataggt ccttcgagcc tcgtcaatca ccatcttctc
     3181 gggattgacc accagccgcg ccgaagtcac ttccggggat gacatcaact ctttgacgtc
     3241 ttcgatcgac tcgaagaccg agtgggcagc aatgaacact tcgtcgtcgg ggagagggag
     3301 atcggtgaga cggctcaaga tcggccgcgc ggcccgcatc aatcgacgct cagtcggcat
     3361 caccttttcg aagtaccagc tcaagacctc ggggagcgac aggaggcgga gggtctccgc
     3421 ggtgggagcg cagtcgacca cgatcacgtc gtacacaccg gatcgagcgt gacgattgac
     3481 atcgagcagg gcaaagagct catccatgcc ggggaagacg aggaattcct ctgcctcgat
     3541 gccggctacg ccgccccagt cgagcacgtc catcagctgg cggcggatcg agccccagta
     3601 tttgtccagc tggcgctggg tgtcgatctg ctggccgtcc agaccggaaa ccaccttcgt
     3661 cggatcgctg ccgaggggca cctcgagggc gtcagccagc gaatgggcag ggtcggtcga
     3721 cgtgaccagg gttcgatagc ccgcgtccgc cgatgccagg gcggtggagg cagcgactgt
     3781 ggtcttgccc actccccctt tgccggtgac caggacgatt cgcatgtcag ctcgcgccag
     3841 tgactctctt gcggagcccg cgaagcgcgg tggtcacgat ctgcttctcg gcctgacgca
     3901 tcaagaagcc tggcattgtg aacttgagac ccaccttcag cccgtactgg acctcggtga
     3961 cgccgtcatt cgcggtgaag gtgtaggagc cctcgagcat ctgcaggtca tcactttcct
     4021 cggcgaccca actcaatcga ttcgggcgat cgtgctcata acgaaggacg tattcgattt
     4081 ccttgacgaa tacctcgatt acgaagcgag cacggtcgac acgaccatct ggcccgcttt
     4141 cgaggacttc gacctccttc acaccggtcg cccactctgg atacgagccg atgtcggccg
     4201 cggcgtcgta gagcacctca ggtgcggcgt ctatctcgag cgtctggaag gttccctcgg
     4261 ccatctctcc acgatccttg tccaggaagc gagactagcg agatgccggg ttagtcgtcc
     4321 cgggacggct cgatgcggag cgggcggccc gttgccctga agtaccccac gttgcgacac
     4381 aacgtcgaac ccacccgcca ggtcgtggct tgaggttggt gcacgtcacc gaacaagtga
     4441 agccggggct gttcccgaac cagatactcc aggatcggag tcgaaccgcg ctcggcgcgt
     4501 ccggtgatca cgtcggtcct aagcgaatgg actgccgccg ggacatgggt gcagaggacg
     4561 tcgacgggcc ctaactgctc gaggagggag gtcatctcgt catcggagat ctctccttcg
     4621 gcctgaagtg gtgtcggggc tccgccgccg acgaatccga acgtgaggcc ctcgagttcg
     4681 acaacctgcc cgtggacgta ttcgaacccg ctcggaagac tggctcgaag acttcgcggg
     4741 cgatcgacat tcccgtggat cacataaccg ctaccccctt cgagggcacc ggaaaccccc
     4801 tggtactggg cgtcgatcgc ggcgccgatc ttctcccgga cctcgtcgta ccggtcctcg
     4861 gcgctatcgg cccagaggtc cctcatcccc tgataatccc cagcagcacg agcctcggca
     4921 gtcgcccggg cgaactcgag accgaggaca tcggcgaccg caccctctcc cgtgcggtag
     4981 tcggtcagat tcacgagatc gccgaggatc aggaccggcg cccccatgcc gaccaccctg
     5041 tgcagggcgt cgagggcgaa atggacgtca gaaatgacga ggatcaattg tcggccaccc
     5101 cggcgccgta gtacccccag atcgcccctg cgatgccacc cgtgagcgcg aggaggaggg
     5161 caatcgccca atcgaaatcg agaggagcaa attcggcgga cagcccggcc atgccaaacc
     5221 cgaccaatcc gccgatcgcc gccctgccgg tcttgcccag tctctttccc ggatcggcct
     5281 gttcgcggtt cagattctcc gcaaatgcgc ccccgaggat cacgactaga agcccgatca
     5341 ctccaaccgc aagagcaact gcatacacct tgaccatgcc cggcggagcg taacggtcgc
     5401 tactccattt tctttgacgc cgaccccaaa gggctcagcc ggtcttccgc tccacgggga
     5461 ccaggcgaag ccgcgggacc aggccctcac cggccaggtt tcgcaaggcc tcgctctctg
     5521 actcgtcgag ctcgagcgga cctccgtcca ggagaaaggt cacgacgcaa tcgtcgcaag
     5581 cactggtgtc ccgcatcgca cattgctggc aatcgatgat caattcgtac ctcctgtcga
     5641 agaaggtacg aatccccggt gacagatttc ctactcgacg aagccgtgct cggccagcca
     5701 ctccccggcc agcaccgccg ggtcctcttg atcgaggtcg gcacgcttgt tgagctcggt
     5761 caggtcctcg gtggtcaggg cctccgagta cgagtcgagg agggccacaa gttcgtcgcc
     5821 gtaggcctcc acgacctcgg tccggatcgc cggcaccagg ttctctgccg ggttcaaccc
     5881 cttgtcgtca gccaacgaga cccagccgtt ggcagcaatg atcccttgtg tgctgaacag
     5941 cagcgccacg tcgatctcac cgccttccag ggcggcgatc gtgatcggcc ctgcgacatc
     6001 gagtgccttg aattcgctga actccagccc gtagacgtct tcgaggccct gcaggcagag
     6061 tggccgtgcc gggcactccg ggggaccgcc gaagatcatc tccccggcca caggttgcag
     6121 atccgacaca ttctccagcc cgaactcgga tgcagtctct gctgtcacca catacgcgtt
     6181 cttgtcctga gccgggctga aagcgagcag tgtcaccccg agctcgctat aagcatcccg
     6241 tgccgcctcc catgtggttt gcgggtcggc agactgctcc acctccagaa agccggccac
     6301 ggttcccgtg tactcgggaa caaggtcgat ctcacccgat tccagagccg gcttcacaac
     6361 ctcccgggct cccaggttca tccgccgctc gatcgcgtac ccgttggcct ccaggccctg
     6421 ggcatagatc tcggcgacga tcaccgattc gttgaagttg aacgacgcaa ccttgatcgg
     6481 ctcgccttcg gtagcgccgt cacccgaccc acaagccgtg gccagtaggg ccaggctgag
     6541 gatcgcccca acggcgcgga agcggaatgc tttccgcatg ggtctctcct tctattggat
     6601 tttgaagagc cccttctcgg gggctctgaa ttgcaaccgt agacgccggt ctccgattcc
     6661 ccctcacctg cctacccgca accccctagg tgtagtcgcc cgttgcgtcg cggagagccc
     6721 gagctcggcc gcgatcgcga gcatcccgac caggatcgcc ccgacaatca gctcaccgac
     6781 gtcctgttgg gcaaaaccgt ccacgatgaa gcggcccaag cctccccagg cgacgagggc
     6841 tcccagagtc gcggtcgcga tcacctgaac gaaagcgatc cgcacccctt ccatgatcag
     6901 aggcatcgat agcgggagct ccacgccagt cagcacctcc ctctcggtca gacccatgcc
     6961 ccgagccgat tccagcgtcg tcggctccac cgtctggatc ccggcgatgg tgttcgtgaa
     7021 catgggcggg gccgccaggg cgatcagagc taccagcact ggccagggct cgccgatccc
     7081 ggcgccgatc tggacgaaga tcaagaaagc gatggcgagg acgccgaaag aggggaccgc
     7141 tcgcccgatg ttgaccactg ccgtggctat ggctgagccc ttacgaatat ggccaagcca
     7201 gacagccaac gggagcacca ggatcccggc gatgacgacg gccgccaccg aaaccgcgat
     7261 gtgctcgccg agccgcaccc agatcgagtc gggccccgac catgtcgacg gctctgagag
     7321 gaattccctc atcgactccc agaccgtcat ttcgggctcc tcgcccacgg actgagggca
     7381 ctctggagcc agatgaaagc gagatcgagg atggtggcta gtaccaccga cagagtcacc
     7441 ccgaccagga tctgggtcca gaagaaagtg ttgaggcccc ggaggatgaa gtatccgtag
     7501 ccaccgaggc ccagcaccgc cgtgaccgtc accaggccga ccgtggtcac ggtggcgatc
     7561 cgaaggccgg cgattatgac cgggagcgca aggggaatct ccatcctcca gaagcgggcg
     7621 cgtcgggtgt accccatgcc gtctgccgcc tccaggaccg gttcgggcac cccgttgatc
     7681 ccggcaacca tgttccggat gatgatgagg agtgtgtaag aggtaagcgc gatgactgca
     7741 ttggttgcgt tcagcccaac gattggcacc aggagggcga aaagcgccaa tgacgggatg
     7801 gtgtacagca agcctgtcac ccatgtgatc ggtgtgtagg tgcggcgcca gcgaaggact
     7861 actgcggtca gccccagaga aatcaccagc cccaaagcca cggagaccgc ggtcaacgac
     7921 aggtgctgcc aggtgcggtc gaggatctct cccatgttcc tccagatcca ttgccagtcg
     7981 atcagaggtg atgctcgcgc catcactccg tgatctcctc ggatatccct tccaggtcca
     8041 gcatcccgcg gtaaagaccc tggtcgacca ccatcgcgac ccgggcatgg ctggtcacca
     8101 cggcatcgag cgccgaccgc agcgacgcat cactacccaa ggtgaccagg aaaggccttg
     8161 ggacgaggtc gcgcagtggt ccggtcccaa ccgccgagcc gtccacccaa cccaggagcc
     8221 gcccttcctc gagaaggcca atccagtcgg tcgattcctc ctccatcact ctcttcgcct
     8281 cctcggggct taccgagacg tcgaccaccg gtccccacct gattccctgg gcctcaccca
     8341 ccggcagcag tgcaagtcgc ttcagccccc gctccccccc gaggaagtcc tcgacgaagg
     8401 cattggcagg gttggcgagg atcgtggctg gctcgtcgaa ctgctcgagc acgccaccca
     8461 cgttgaggat tgccatgcgg tcaccgagga ggatcgcttc gtcgacgtca tgagtcacga
     8521 acaccacagt ctttccaagg cgttgctgca aggcttgaaa ctcatgctga aggcgcttgc
     8581 gaacaatcgg atcgacggca ccgaagggct cgtccatgag cagaagcggc gggtcggctg
     8641 ccagggcccg agcaacaccg acccgttgct gttgcccacc ggagagggcg gccgggtaac
     8701 ggtcgagcat ctcggggtcg agaccgacca aggccgccag ctgctcgatc cgctctttga
     8761 tcctgcccgc gtcccacccc atgagggcag ggacggtggc gatgttctgg gcgatagtgc
     8821 ggtgtgggaa caacccgacc tgttggatca cgtagccgat cgagcgtcgc aactcatgag
     8881 gtggaacggc gacgacatcc gcaccgtcaa tcaggattcg tcccgaggag ggctcgatga
     8941 tccggttgat catcttgaga gtggtggtct tgccgcatcc cgaagggccg accagggtca
     9001 cgaactcccc tttgccaaca tcgaggtcca gatcgtcgac cgcgggtctg gaggcgcccg
     9061 gaaacacctt ggctaccttc tccaggcgga tcatggtgtc ggtcaagtcc tgaggtccca
     9121 cggtgggtga gaccctacgt ccggccgggg tagctcaccc gacttcttgg atgaacccca
     9181 ggacttctga gatgtacttc tggggatcca cgtcatatga cccgacatgg tccgcaccct
     9241 cgacctgaat caactgcacc agatcgggct gggtctcttg gagctcgatg ctttcgctga
     9301 tcggaactgt cagatccgcg gtcccgtgat ggacaagcac cggttgacgc aacgagtcgg
     9361 cagccttctc gacgtagtcg actgattgcc agttgaactc gatgcgaagc gaggtgaaga
     9421 actgggccac cgtgctcaga gacgtcggga cgttgaacgg cagaatcggc agttcacgtt
     9481 gcgaggcgcg atagtcgacc gtctcggcca gatcgatatt gggtgagtcg agaagcactc
     9541 ccccaactgt gtccagagac tggtgataca tgaacgagag aacatgggca ccgccggtgg
     9601 agtagccgtt gagtatcacg cgctctgctc cgttgtcgag ggcgtaggtg actgcgcctg
     9661 cgacgtcctc ccactcggtg actccgtact ggtagtagcc gctcgggtcg accggttgcc
     9721 cgtcgtcgtt tcggtaggtg atggccaatt gggggtatcc agcctcttgc agcggggcga
     9781 acatcggctc ggcctcggca ggtgtcgtcc ctttgccatg gacgtggatc acccaggtgg
     9841 tgccagctgc aggtaggtac caggcggcca tcgacccgag tggtgaggcg tatgccactt
     9901 cctcgagctg atagctgccc gaaggaacca ccaaaggctc tgcctggatc tcgaaaccgt
     9961 cgtcgatcag ctcaccggag aagtaccagc cgcccacgac gtggaagacc agggcaaggc
    10021 caaccaaggt ccaaaaggct ccccagagat aggttcggag cgtcacgctc ggtgattctg
    10081 cccggattca ggccgcaggc aatgacaaac ctccaacgag gtgctcctca tgatcggtca
    10141 ccggtatcgg gtcctcgccg aatgtcggct ccagttcccg cgcttcggcg agggtctcgt
    10201 cgaccttcgc cgacaccgct tcctccatga tcgagccgac aacggtgtca ccggactgct
    10261 ccagccggtc tcgacgcacc agtccgatga ggacgccttg atcggctacc gccaacactt
    10321 tctcggtgtg gatgcgcagc gtgtcgtttg cctggcggag gggcccaacg aagaccgggt
    10381 cacccatcca gcgaaagacc cgctcgggag cgctgcggac catgacggcg ccggcctggg
    10441 ccttgcttcg caagcgggct cccggtccaa gcatcgaacg ttggagccac gatccacccg
    10501 gagcgccaag gacgagtagg gagccttcgt cgagatcctg ggcaagactg ctgatgcctt
    10561 ccacgcccac aacccgcccc ttgagtcgag ggacgacggc agcgatgcga tcgagggtga
    10621 gctgggcagc agagggatcg tcacccggcc cggaggcaga caccatctcg gcttccacgc
    10681 cgaggctggc tccgaccaga gcggcagtct gtgcggcaag gatcgagtgc gggccaccag
    10741 caacggtggc caccaccccg gtcacattct cgggcacgaa ggcgaccgga acggccacat
    10801 tgaccaggtc gaaggaaccg ggaagtttcc tcgagacgag aagatcggcg cggtagctga
    10861 tgcaggcctc gaccaggtcg ccagtgatgc ggttcgactg ggcaccagcg aggagatcgg
    10921 ccaactcgga gcaccccttg ccccgggtcc acaccacgga gaatccgcct ccggcttgcc
    10981 caccgaccgt cattcgtctc cctccatgat ccgcacccct caaaagctcc tcaacgttaa
    11041 gccctttgcc gggcattgtc cccgacctgc tcccgacatc atcgtcagcg ggagtgacaa
    11101 gatggagcga tggagttcaa agaagtgatc gcggcacgcc gaatggtgcg caactatctc
    11161 cctgatccgg tggcgaggga gagtctggat cgcatcgtcg attccgggct tcgggccccg
    11221 tctgccggct tctcgcaagg tcttggattt gtggtggtga ccgaaccaga gatgcgtcag
    11281 gccatagcgg agcttgctga tgaggccgaa tatgtggcca gcggtttcga tccctggatc
    11341 tcgcgcgcac cggcacacat catcatcacg gtcagcgaaa aggtttacag ggaccgatac
    11401 cgggagcctg acaaactcgg accggacggc gcggagatcg agtggccggt cccgttctgg
    11461 tgggtcgacg ccggcgcggc gttgatggcg gtcctccttg cggccgtgga cgaaggcctg
    11521 gccgcgggct tccttggtgt gcactcgctc cccgaccttg ccactctcct ccggctgcca
    11581 ccggatcacc ttccgatcgg agtggtgacc attggggccc ctgctcccga tcgccggtcg
    11641 tcgagcctca ctcgtggctg gaaacgacgg gagtcggtgg ttcactcgga aacatggggc
    11701 caatgaccga tctcgcgatc acttcgcacg cgaatccgcg gatcaaggcc ttggtcggac
    11761 tgcgggatcg aggcgcccgg gaccgccaga gattgttcgt ggtcgagggg cctcgccttg
    11821 tggaacgggc gttggcggcc ggacatcggc cggtcgaggt gttttacgac cccaatcgct
    11881 tcgaccccgg cccgctgacc ggccccaccg tcttcccctg ctccgccgag gttctctccc
    11941 gtgcctccta ccgggcgtcg gatgaggggg tcatcgccgt ggtgcctcag ttcgatctcg
    12001 atctgagcgg gctcgccctg cccgaggtgc cgttgatcct ggtggctgaa ggcatcgaga
    12061 agccggggaa cctgggggcg atcctccgca cggctgacgc agtaggtgcg gatgccgtgg
    12121 tcgccgtcga tcccgagatc gacccattca atccaaatgt ggtgcggtca tcgaccggag
    12181 cgctgttcac cgtccccatc gcagtgtccg gtctcgagaa cgccctccac tggctgcatg
    12241 aggccgggat ccgcctcatt ggtgccgacc cgagtgcaga ccacgacctc tggtcgatcg
    12301 acttcacaca gccctgtgcg ctcatggttg gttccgagca tggtgggctc accgaccgcg
    12361 tacggcaagc ggcggatggg ttggcgtcga ttccgatgcg aggggccacc gattccttga
    12421 acgcatcggt gacgatcgca ctgctcgcct acgaagccct ccgtcaacgt cgagcctgat
    12481 acccactctt gacatcgaac gtatgttcgg gttagcctcg aatgggccgt gtccgccctg
    12541 ctccaaccca gtttcgacga gctctccacc ccgctctacg cagtgacctt ttgcgtgctc
    12601 gacttggaga caaccggcgg ctcggccaag gactgcgaaa taaccgagat cggggccgtc
    12661 aagtaccggg gtggcgaact gctcggaagc ttccagacct tggtggaccc cgggctcccg
    12721 atccctccct cgatcacgat tctgaccggg atctcggagg caatgatctt cgacgctccc
    12781 aagatcgagt ctgcgtttcc caccttcctc gaattcatcg gcgatgcggt ggtcgtcggg
    12841 cacaacgtgc gattcgacat gtcgttcctc caggccgccg cgacccgatt gggttttgag
    12901 cgaatggcca acgacaccgt cgacactgcc gctctggctc gccgactggt acgcgacgag
    12961 gtacgaaatc tgaggctgcg caccctggcc gcccatttca ggagcccggt gaccccgatc
    13021 cacagggctc tggacgacgc ccaggcgaca gcccacgtgc tccacgcatt gctggagagg
    13081 gtcggcaacc tcggggtcac caacctcgac gacctgctcc agcttcccaa ggctcggggc
    13141 tctgccttct actccaagat cagcctcagc gaagggcttc cccgccggcc cggggtctac
    13201 atcttccgcg accgccacgg gacaccgatc tacgtcggca aggcgaccaa tctccgagcc
    13261 cgggtgcgcc agtacttcta cggcgacgaa cgacgctcga tcgggaacct gatgcgcgaa
    13321 ctggtgtcga tcgatcatgt cctctgcgag acgccgctcg aggccgaggt caccgagctg
    13381 aggctgatcc acgcccatcg accccggcac aatcggcggt cgaggccccc gaagacctcg
    13441 cactgggtga agctgacgcg cgagccgttt cctcgacttt ctgtggttcg caccctcaag
    13501 gaggatgggc tcgcccacct ggggccattt cgctcgaaac aggcggccga tctcgtcgtg
    13561 accgcccttt gggatgcgac cgagattcgc cggtgccggt cctcggcttc agaacgaagc
    13621 ggtccgtgtg cagcagcaca actcggcgtc gcagcctgcc cctgcgatgg ctcactgctc
    13681 cccggggact accgcccgat cgtcgagcgg ctgatcgagg gcctcgatcg caaccccgcc
    13741 ctcctcctga ccccactggc cgagaagatg gccgaattgg ccgatcagca tcgatacgag
    13801 gaggccggct ggttgcgaga ccgtcaccac gccctgtccc gggcgatcga gcgccgccag
    13861 gtctggtctg ccctcagcgc ctttgggctc tgcgaggtgg agagtcgaga cggttgtcga
    13921 gtattgatcg accatggaaa gctggtgtcg acctggcgcc tggaccggac acctccgctg
    13981 cgcccggcgc ccgaagtcgg cgagcatggc aatcgccagg tcccggagag tgtcgagatc
    14041 gccgaggaag ccgacctcat atggaagtgg ctcgacacca ccgagatacg actggtcgat
    14101 gccaccgggc tcctggccct ccccgttcac caggtgcctc gccttcgggc cagttgacct
    14161 gacttgagtc tcccccatca gaccatgaga cttgatccgt gggtcgtctc gtcgatctca
    14221 accaaatagc agtctccctg ggcgggcttc ccgtccttcg tgacatagag ctcgccattg
    14281 atgccggcga gacactcggc gtgagcgggc ccaacggtgc gggaaagacc actctgcttc
    14341 gggtcctcgc aaccctgctg gcgcccatca ccggttcggg cacagtcctg ggagccgaat
    14401 tggggacagc agcggtctat ccggttcggc gcagtatcgg gctgctctct catcgacctg
    14461 ccttgatccc agagctgacc ctcgccgaga acctgcgcca cgcagccaat ttgtctggca
    14521 tcgaattcag caaggcagaa accgccttgc gagtagttgg cctggatgag gtgtcctcga
    14581 ggacggccgc cgccagctca ttcggcatgc tccgaaggac agaggtcgct cggctcttga
    14641 tcacctctcc gacgatcctc ctcctcgacg agccattcac cgggctcgac gtcgaagccc
    14701 aggagctcat cggggcgctc gtcacccgaa ctaccgggga
//
LOCUS       DBNHXU010000398         4220 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3937083, whole genome shotgun sequence.
ACCESSION   DBNHXU010000398 DBNHXU010000000
VERSION     DBNHXU010000398.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4220)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4220)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4220
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3937083"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..543
                     /locus_tag="AC161X_09755"
     CDS             <1..543
                     /locus_tag="AC161X_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014445224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HIR4989881.1"
                     /translation="DEFTSMLAGAMGSLKMGPGIDRSNQVGPMINERSRDDIAKLVQG
                     AVDEGARVVTGASVPDRQGFFYEPTVLSGVAAGSDILQQEIFGPVAPVVTFETDEEAI
                     ALANDTVYGLAAYLYSGDLARGLRAAEGIEAGMVGVNRGLVSDPAAPFGGVKHSGVGR
                     EGSHEGMDEYLETKYVALNW"
     gene            complement(567..1028)
                     /locus_tag="AC161X_09760"
     CDS             complement(567..1028)
                     /locus_tag="AC161X_09760"
                     /inference="COORDINATES: protein motif:HMM:NF037170.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HIR4989882.1"
                     /translation="MNDLDQGYAFWSAVTGLEVIGRDPGGWHGRFGYLGRKDPWKHEL
                     ILQVVDSSKGERPNRAHIDVTPNKGIDDAITRILSLGGSVKKPPSLYPRPGSHGDEPP
                     IIDWAVMQDPFGNEFCLVDDLSDAQIEAVAKATAGGASTDEEWRTAANGTR"
     gene            complement(919..2382)
                     /locus_tag="AC161X_09765"
     CDS             complement(919..2382)
                     /locus_tag="AC161X_09765"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014741.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease signature motif containing
                     protein"
                     /protein_id="HIR4989883.1"
                     /translation="MFESEGIPKGLAEMAPGPQLAAILLSIDRDRITGRERVILMKAR
                     SRLRAWVESDLYADLLSVAAAEAEIFGQPEEAMEAAAGEIQAALNLTRRSADIQMGLA
                     YQLGDRLPDVWAALHAGSIDLARARVLCDETMHLPIEDARRIAEIALERAPRQTTGQL
                     GARIRRLVIALGPDLAKDRYTKATEDRRVVLEPNHHGTSNLTGLELPAADAQAAMGRI
                     NTLAKAAKSADDPRTMDQVRADVYLDLLNGRHLKQSRGRGMVDMTVELTTLAGLDDHP
                     GDLNGYGPVIADVACQVAATQADVEHRVTVTEHGEPIWTGITRRRPTSSQKRYVQARD
                     RTCVFPGCRMPATECDIDHTIDYSQGGPTTPWNNAPLCRHHHRGKHKRGWKLERTAPG
                     AYKSAIPTPSSPNRRSTQGPRHVSSTTNGATPLRAGTARSDPTRYTNCRDRQLSRDRH
                     RRERPRSGVRLLVGGHRSRGDRARPGRLAWPIWISRA"
     gene            complement(2508..3971)
                     /locus_tag="AC161X_09770"
     CDS             complement(2508..3971)
                     /locus_tag="AC161X_09770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017668167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HIR4989884.1"
                     /translation="MATTRVEISEYAVSPDHYIGGKRVDSPETFEVRSPLDWDWKLAD
                     IAQGDPLTSDQAVSAAVAAFPGWAALAPKERGEHLHRLADLIDQNIEAIAQVECVDMA
                     MLLESLQARVIHRGAENFRNYADLAAEWEGRRWSSKGTDNQVQRMPAGPAVIITPWNA
                     PWILSTWKIAPALAAGNPVILKPAEWAPLSASILADLADQAGIPPGVLNVVQGIGEEV
                     GAALVSDRRVRRISFTGSPETARHIGIAAASNIVPFTAELGGKGPFLVFADSDLEAAA
                     RKAAGQYDDAGQVCLAGTRLLVEESVSSDFLELFHRFTDAHVLGDSRSPQTTISPLIH
                     PDHLARVDGFVKRARDAGDRIVRGGERWKQDGLWYEPTLIEPKSNDSEVVQREVFGPV
                     LTYQTFATEAEGIALANSTQYGLSGIVYTGSEERAERVGRGVRAGTIWVNTFLVRDLT
                     APFGGFRISGIGREGGDYALDFHSDLKTLQVNQGTVG"
     gene            4019..>4220
                     /locus_tag="AC161X_09775"
     CDS             4019..>4220
                     /locus_tag="AC161X_09775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HIR4989885.1"
                     /translation="MPRPLIDHDPEVVLEAERDIRARLGDRKLDFDALLAVSNIYRAA
                     NAVRNRMEREVLAPEGLSWGGFT"
BASE COUNT          717 a         1310 c         1395 g          798 t
ORIGIN      
        1 gatgagttca cctcgatgct cgccggtgcg atggggtcgt tgaagatggg tcccgggatc
       61 gaccgttcca atcaggtcgg cccgatgatc aacgagcgct cgcgtgacga catcgccaag
      121 ttggtgcaag gtgccgtcga cgaaggcgcc cgggtggtga ctggagcctc ggtacccgac
      181 cgtcaggggt tcttctacga gccgaccgtc ctcagcgggg tggcggccgg ctccgacatc
      241 ctccagcagg agatcttcgg cccggtggcg ccggtggtga ccttcgagac cgacgaggag
      301 gcaatcgcac tggccaacga cacggtgtac gggctcgccg cataccttta cagcggcgac
      361 ctggcgcgag ggctgcgtgc ggcggaaggc atcgaagcag gcatggtcgg ggtcaatcgc
      421 ggtctggtct cggacccggc ggcgccgttt ggcggcgtca agcactccgg cgtaggtcgg
      481 gaagggtccc atgaggggat ggacgagtac ctggagacca agtacgtagc cctcaactgg
      541 tagagccggc ctctggccga ttgtttctaa cgcgttccgt tggcggcggt ccgccattcc
      601 tcgtcggttg atgcaccgcc cgccgtggcc ttcgccaccg cttcgatctg ggcatcggac
      661 aaatcgtcga ccaggcagaa ctcgttgcca aacgggtcct gcatgaccgc ccagtcgatg
      721 atcggtggtt cgtctccatg cgagcccggt ctcgggtaca agcttggcgg cttcttcacc
      781 gaaccgccaa ggctgaggat ccgggttatc gcgtcgtcga tgcccttgtt cggggtcacg
      841 tcgatgtggg cacggttggg ccgttcgccc tttgaactgt ccaccacctg aaggatgagc
      901 tcgtgcttcc acggatcctt acgcccgaga tatccaaatc ggccatgcca accgcccggg
      961 tcgcgcccga tcacctcgag acctgtgacc gccgaccaga aggcgtaccc ctgatcgagg
     1021 tcgttcacgt cgatgacggt cgcgcgaaag ctgcctatcc cgacaattgg tgtagcgagt
     1081 gggatcggag cgggccgtac ccgctcgtaa tggggtggct ccattagttg tcgacgatac
     1141 gtggcgggga ccctgggtgc tacggcggtt cgggctcgac ggtgtaggta tggccgattt
     1201 gtaagcgccg ggtgcggttc tttccagttt ccagcctcgc ttgtgtttgc cacggtggtg
     1261 gtggcggcaa agtggggcgt tgttccacgg ggtggtcggc ccgccttgtg agtagtcgat
     1321 ggtgtggtcg atgtcacact cggtggcggg catgcgacat ccggggaaga cacaggtccg
     1381 gtcccgtgct tggacgtatc gcttctgact gctggttgga cgtcgccggg tgataccggt
     1441 ccagatcggc tccccgtgct cggtaaccgt cacccgatgc tcgacatcgg cctgagtggc
     1501 ggcgacctgg caggcgacat cggcgatgac gggtccgtag ccgttgaggt ctccaggatg
     1561 atcgtcgagg ccggccaggg tggtcagctc cacggtcatg tcgaccattc cccggcctcg
     1621 actctgcttg aggtggcggc cgttgaggag gtcgaggtag acgtcggccc gaacttggtc
     1681 catggtccga gggtcgtcag cacttttggc agccttggcc aaagtgttga tccgtcccat
     1741 tgcggcttgg gcatcggcag cgggtagctc gaggccagtg aggttggagg tgccgtggtg
     1801 gtttggctcg aggaccaccc gacgatcctc cgtcgccttc gtgtagcgat ccttggctag
     1861 gtcggggccc aaagcgatga ccaatctccg gatccgggct cccaactggc cggtggtctg
     1921 gcgaggtgcc cgttccagtg cgatttcggc aatccgtcta gcgtcttcga tggggaggtg
     1981 catcgtctcg tcgcagagca cccgcgcgcg agccaggtcg atcgacccgg cgtgcaaggc
     2041 ggcccaaacg tcggggaggc ggtcgccgag ttggtaggcc aaccccattt ggatgtcggc
     2101 agaacggcgg gtgagattga gtgcggcctg gatctcgcca gcggcggcct ccatcgcctc
     2161 ttccggttgg ccaaagatct ctgcttcggc agcagcgacg gacaacaggt cggcgtagag
     2221 atccgactcg acccacgccc ggagccggga ccgggccttc atcaggatca cccgctccct
     2281 tccggtgatc cggtcccgat cgatcgacaa caggatcgcg gccagctgcg gaccgggcgc
     2341 catttccgcc agtcctttgg ggattccctc gctctcgaac atatgttcgt taccctacaa
     2401 acccccaccg acagattcca tacccctgtt caggaatttg gaagatttct ttgcggcatg
     2461 cgaacccccc gtcccccctt gaataggggg gacatcggaa gcgctcctca gccgaccgtc
     2521 ccctggttga cctggagtgt cttcaggtcg gaatggaagt cgagggcgta gtcgccgcct
     2581 tctcgcccga tcccggagat tcggaacccg ccgaatgggg cggtcaagtc acgcaccaag
     2641 aaggtgttga cccagatcgt ccccgcccgg actccacggc ccacccgctc ggcccgttcc
     2701 tccgatccgg tgtagacaat ccctgagagc ccgtattggg tggagttggc caaagcgatg
     2761 ccttcagcct cggtggcaaa ggtctggtag gtgagcaccg gcccgaacac ctcccgttgc
     2821 accacctcgg agtcgttcga cttgggctcg atcagggttg gctcgtacca gaggccatcc
     2881 tgcttccagc gctccccgcc gcggacgatc cggtcacccg cgtcccgcgc ccgtttcacg
     2941 aacccgtcga cccgcgccag atggtcgggg tggatcagag ggctgatcgt cgtttgcggg
     3001 ctccggctgt caccgagcac atgggcgtcg gtgaaccgat ggaacaactc gaggaagtcg
     3061 gacgagaccg actcctccac gaggagccgg gtaccggcga gacaaacctg cccggcgtcg
     3121 tcgtattggc cggcagcctt acgggcagcg gcctccagat cagagtcggc gaacacaagg
     3181 aaaggcccct tgccaccgag ttcggcggtg aacggaacga tgttcgacgc agcagcgatc
     3241 ccgatgtgtc gcgcagtctc cggtgagccg gtgaatgaga tccgtcgcac ccggcggtca
     3301 gagaccagag ccgcgcccac ttcctcgccg atgccctgaa ccacgttgag caccccggga
     3361 ggaatcccgg cctgatcggc gaggtcggcc agaatcgagg cggagagcgg ggcccattcc
     3421 gcgggtttga ggatcacagg gttgcccgcg gcgagtgcgg gggcaatctt ccaggtggac
     3481 aggatccaag gggcgttcca aggagtgatg atcaccgccg ggcccgcagg catccgttga
     3541 acctggttgt ccgtcccctt ggacgaccag cggcgaccct cccactccgc ggccagatcg
     3601 gcatagttgc ggaagttctc ggcgccacga tggatgaccc gcgcctgaag gctctcgaga
     3661 agcatcgcca tgtcgacaca ctcgacctgg gcgatggcct cgatgttctg atcgatcagg
     3721 tcggccaggc gatggaggtg ctcgccccgt tccttcggag caagtgcggc ccaaccgggg
     3781 aaagcggcca ccgcggcgct aaccgcctga tctgatgtga gtggatcccc ttgcgcgatg
     3841 tcagcgagtt tccagtccca atcgagcggg gagcggacct cgaatgtctc gggcgaatcg
     3901 actcgtttgc caccgatgta gtgatcgggg gagacggcgt attcggagat ctcgactcgt
     3961 gtggttgcca tagggatcgt tcgcaaccta aggataaact gcgctcacca ggactctcat
     4021 gccaagacca ctgattgatc acgatcccga ggtcgttctc gaggcggagc gagacattcg
     4081 cgcccgtctt ggcgatcgga aactcgactt cgacgccctg ctcgccgtgt ccaacatcta
     4141 tcgcgctgcc aacgcggtgc gcaatcggat ggaaagagaa gtcctcgccc cggaagggct
     4201 gtcctggggc gggttcacga
//
LOCUS       DBNHXU010000399         3032 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3539287, whole genome shotgun sequence.
ACCESSION   DBNHXU010000399 DBNHXU010000000
VERSION     DBNHXU010000399.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3539287"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..240)
                     /locus_tag="AC161X_09780"
     CDS             complement(<1..240)
                     /locus_tag="AC161X_09780"
                     /inference="COORDINATES: protein motif:HMM:NF036859.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989886.1"
                     /translation="MTTTRHSTLTNWGAYEVETDGREITAVHPFAKDPDPSPIGQSLK
                     AVRKSRVDKPSIRESWLRDGPGSNPEGRGQEPFVEV"
     gene            complement(300..1046)
                     /locus_tag="AC161X_09785"
     CDS             complement(300..1046)
                     /locus_tag="AC161X_09785"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR4989887.1"
                     /translation="MAVQSIDRAFQVLKALAIEPSGVTDLAQRVGLPKSTVARMLSSL
                     EEQGAVAKGTDGNLYSIGMGLVELAGAIDATAALATAVRPHLRGLSDQLGEASGFSVP
                     TGYTIQYMVQVESPNPVQVRDYTGLAVPMHIGPSGLCVMAQWPREEVDRYLKRSLERY
                     TPKTVVEPSQILERLEAIRRVGHCWIYEEFAEGINSVAAPVLIEGSLAMGAIHVHGPA
                     YRFPTEGKADSIAEMVMDAAARFSDRPEVI"
     gene            complement(1046..1867)
                     /locus_tag="AC161X_09790"
     CDS             complement(1046..1867)
                     /locus_tag="AC161X_09790"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase [NAD(P)H] activity [Evidence IEA];
                     GO_process: GO:0006555 - L-methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HIR4989888.1"
                     /translation="MSESLVKALLTGADFEVIPLKSVMDQVATIPPGSTVSVTASPAK
                     GMKTTVDLAIELQNQGYRAVPHLSARMIEGRQDLGSIIKRLDDGGVSRAFITGGDAEQ
                     RGIYFDALSLLLDLAELGHPFFEVGITGYPEGHPAIPEDRLRKALVEKQAHATYIVTQ
                     MCFNPKAIVDWVNTIRADGVTLPIKVGVPGAVEPARLLTIGARIGVGDSLRFVAKNRR
                     ALFRILRPGRYRPDRIIEPLGRLGEGLGLTGIHVFTFNQVGPTVAWHNKALARLG"
     gene            complement(1871..>3032)
                     /locus_tag="AC161X_09795"
     CDS             complement(1871..>3032)
                     /locus_tag="AC161X_09795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015451802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HIR4989889.1"
                     /translation="AYGSPFVRDLEGGRRYATIGDFENFARLTHMTSSLHHGGGTLCE
                     PVDLPVNKRHFDMVFAHIKNHDKAFMGSVTAPQRAQDTVDMADIAFGGEAKAGKTVLL
                     SLCNANSPMSWDYNMLGSAKIYAENNQACLMTPFILAGAMAPVTVAGTATQTLAEAMA
                     GMAFCQIVRPGAPVVFGSFASSISMQSGAPTFGTPEPALVLFVMAALARRLGIPFRSG
                     GNLTAAKTPDYQAAYESAATFLPPILAGVNFVLHTAGWLEGGLAMGYEKFILDADQAG
                     MAEAILGGIDLSENGQALDALLDNPPGQHFLGNAHTLANFESAFWRSGTADNNSFEQW
                     QEAGSQEAVSRASAIWRRMLTEYEPPALDEAIEAELVDWIGQRKASFPDSDV"
BASE COUNT          551 a          975 c          940 g          566 t
ORIGIN      
        1 cacctcgaca aatggctcct gaccccgccc ctcggggttg gagcccgggc catcccgaag
       61 ccacgactcc cgaatcgacg gtttgtcgac ccggctcttg cggactgctt tcagagactg
      121 gccgatcggg gacgggtcag ggtccttggc gaacgggtgc acggcggtga tctcacgtcc
      181 gtcggtctcg acttcatagg caccccagtt ggtcaaagtc gagtgtctgg tcgtggtcaa
      241 tcggcctccg tatttgcggc caggtcaggc tagttctgcg gtccggtcat gaggctcgat
      301 caaatgactt cgggccggtc ggagaaccgg gctgccgcgt ccatgaccat ctcagctatc
      361 gaatcggctt tcccctcggt agggaatcga tacgccggcc catgtacatg aattgcgccc
      421 atcgccaggc tcccttcgat cagcaccggc gccgccaccg agttgatccc ctcggcgaac
      481 tcctcgtaga tccagcaatg acccacccgg cgaatcgctt cgagtcgttc aaggatctgt
      541 gagggctcga caactgtctt cggtgtgtac cgctccagcg accgcttgag gtagcggtcg
      601 acttcctcgc gcggccactg cgccatcaca cacaaccccg acggcccgat gtgcatgggg
      661 accgccaaac ctgtgtagtc gcggacctgc acaggattgg ggctctccac ctggaccatg
      721 tactggatgg tgtatccggt gggaacgctg aagcctgacg cctcgccgag ttgatctgac
      781 aggcctctca ggtgagggcg caccgcggtt gcgagagctg ccgtggcgtc gatggccccc
      841 gccagctcga ccagccccat tccgatcgag tagaggttgc cgtcggtgcc tttggcaacc
      901 gccccctgct cctccaagct cgacaacatc cgggccactg tcgacttggg caggcccacc
      961 ctctgcgcga ggtcggtgac ccccgacggc tcgatggcga gagccttcaa gacttggaaa
     1021 gcccggtcga tcgactgcac cgccatcagc ccaacctcgc caatgccttg ttgtgccagg
     1081 caacagtggg gccgacttgg ttgaaggtga agacgtggat cccggtcagc cccagcccct
     1141 cacccagacg gcccagtggc tcgatgatcc gatcgggccg atatcgcccg ggacggagga
     1201 tccggaacag tgcccgtcga ttcttggcca cgaaccggag cgagtcccca accccgattc
     1261 gtgccccgat ggtcaacaac ctggccggtt caaccgcccc gggcaccccg accttgatcg
     1321 gaagagttac cccatcggcc cggatcgtgt tcacccagtc gacgatcgcc ttcgggttga
     1381 agcacatctg ggtcacgatg taggtggcgt gagcctgctt ctccaccagt gctttccgaa
     1441 ggcgatcctc agggatcgct ggatgacctt cgggatagcc ggtgatcccc acctcgaaaa
     1501 agggatgccc gagctcggca aggtcgagaa ggagtgagag ggcatcgaag tagatcccgc
     1561 gttgctcggc gtcgccgcct gtgatgaagg ccctggaaac ccctccgtca tcgaggcgct
     1621 tgatgatcga tcccaggtct tgacgccctt cgatcatccg ggccgacagg tgcggcacag
     1681 cccgataacc ctggttctga agctcgatgg ccagatcgac ggtggtcttc atccccttcg
     1741 ccggcgacgc ggtcaccgaa accgtggagc cgggaggaat cgtggccacc tgatccatga
     1801 cggacttcag ggggatcacc tcgaagtcgg ctcctgtgag aagcgccttg accaggctct
     1861 cactcatgat tcagacgtcg gagtccggga aagacgcctt gcgctggcca atccaatcga
     1921 ccaattccgc ctcgatcgcc tcatcgagag ctggcggttc atactcggtg agcatgcggc
     1981 gccagatcgc gctcgcccga ctcacggctt cctgtgaccc tgcttcctgc cattgctcga
     2041 aactgttgtt gtcggcggtc ccgctccgcc agaaggcgct ttcgaagttg gccagagtgt
     2101 gggcgttgcc caggaagtgc tgacccggcg ggttgtcgag aagtgcatcg agggcttgcc
     2161 cgttctcgga gagatcgatg cctccaagga tggcttcggc catgccggcc tgatcggcgt
     2221 cgaggatgaa cttctcataa cccattgcca gaccgccttc gagccatccg gcggtgtgaa
     2281 ggacgaagtt gaccccggcc aggatggggg ggaggaaagt cgccgccgac tcgtaggcgg
     2341 cttggtaatc gggagtcttg gccgcggtga gattcccgcc gctgcggaat ggaataccca
     2401 agcgccgcgc cagggccgcc atcacgaaca ggaccagcgc cggctcgggt gtgccaaaag
     2461 ttggggcccc ggattgcatc gagatcgaag atgcgaaaga cccgaacacg accggcgccc
     2521 ccgggcgcac gatctggcag aaggccatcc cggccatggc ctcggccagg gtctgagtcg
     2581 cggtcccggc gacggtcacc ggagccatcg ccccggcgag gatgaacggc gtcatcagac
     2641 aggcctggtt gttctcggca tagatcttcg ccgagccgag catgttgtag tcccagctca
     2701 tcgggctgtt ggcgttgcac aacgagagca gcaccgtctt tcccgctttc gcctcgcctc
     2761 caaacgcgat gtcagccatg tcgaccgtgt cctgggcacg ctggggagcg gtcaccgagc
     2821 ccatgaatgc cttgtcgtgg ttcttgatgt gagcgaagac catgtcgaaa tgccgtttgt
     2881 tgaccggtaa gtcgaccggt tcgcaaagcg ttcccccacc gtgatgcagc gacgaagtca
     2941 tgtgagtgag ccgggcgaag ttttcgaagt cgccgatggt ggcgtagcgg cgaccacctt
     3001 ccaggtcacg aacgaatggc gacccgtagg cc
//
LOCUS       DBNHXU010000400         1507 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_963138, whole genome shotgun sequence.
ACCESSION   DBNHXU010000400 DBNHXU010000000
VERSION     DBNHXU010000400.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1507)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1507)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1507
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_963138"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..497)
                     /locus_tag="AC161X_09800"
     CDS             complement(<1..497)
                     /locus_tag="AC161X_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HIR4989890.1"
                     /translation="MSDDYPAIAGVTDLTDRLRQGGYLADRGLATSVYVALTLGRPLL
                     LEGEVGVGKTELAKVLATVFDRRLIRLQCYEGIDTNQALYEWDYARQMLQIRALSEHN
                     LADEEQVDKLFGPKFLLERPLLTAVREGDRAVLLIDEIDRADDEFEAFLLELLSDYQI
                     TIPEIG"
     gene            complement(541..792)
                     /locus_tag="AC161X_09805"
     CDS             complement(541..792)
                     /locus_tag="AC161X_09805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989891.1"
                     /translation="MTGQEETTADVLRSVWATTTHDHPLDEDCSPNKACRNMKDVTFD
                     GYAEPGTDIGAAWRAKLAREGKLTTSGGSVRDLVLGQGE"
     gene            complement(803..>1507)
                     /locus_tag="AC161X_09810"
     CDS             complement(803..>1507)
                     /locus_tag="AC161X_09810"
                     /inference="COORDINATES: protein motif:HMM:NF024869.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HIR4989892.1"
                     /translation="VRFEVSEIDALIAGLPTAGVARCLVVPATAFPGELWEMAAARQP
                     LCLVCRIEGDEVAEISLFSDDNIGEAGPDVEQLFQAGASGSTLIDDQVITVLRPVPQL
                     VVVGDTPVGDALAELASLVGWKARMFNDAASATGVIATLSPLDKVVVTAHDLDLAGAA
                     LMAALESEAGYIGALGAHRMQENRADWLAYRGVTDLSRIHGPAGIDIGAETPSEIAVS
                     ILAEAIGAGVTDPVSD"
BASE COUNT          262 a          469 c          497 g          279 t
ORIGIN      
        1 ccgatttcgg ggatcgttat ctgatagtcg gagagaagct ccagaaggaa ggcttcgaat
       61 tcgtcgtcgg cccggtcgat ctcgtcgatg agcaacacgg cgcggtcacc ttcccgcacc
      121 gcagtgagca ggggccgttc gaggaggaac ttgggaccga acagcttgtc gacttgctcc
      181 tcgtcggcca ggttgtgctc ggaaagcgcc cttatctgga gcatctggcg ggcgtagtcc
      241 cactcataga gggcctgatt ggtgtcgatt ccctcgtagc actggagccg gatcagacgg
      301 cggtcgaaca ccgtcgccag cactttggcc agttcggtct tgcccacgcc gacttcgccc
      361 tcgagcaaga gtgggcggcc cagggtcagt gccacataga cggaagtggc cagtccccgg
      421 tcggcgaggt acccgccctg gcgaaggcga tcggtgagat cggtgacacc ggcgatggcc
      481 gggtaatcgt cactcatcgc atgagcacca atcgatggga agtggccgct ttggtcaggg
      541 ttactcgccc tgaccgagga ccaggtcgcg gaccgacccc ccggaagtgg tcagcttgcc
      601 ttctcgggcc aatttggccc gccaagctgc gccgatgtcg gttcccggct cggcgtagcc
      661 gtcgaaggtc acatccttca tgtttcggca ggctttgttg gggctgcaat cctcatcgag
      721 tgggtggtca tgggtggtgg tcgcccaaac cgaccgcagg acatcggcag tggtttcttc
      781 ttggcccgtc ataaggacaa gtctaatcgc tgaccgggtc ggtgacaccc gcgccgattg
      841 cctcggcgag gatggagacg gcgatttctg agggggtctc ggcgccgatg tcgatgcccg
      901 cagggccgtg gatgcggctg agatcggtca ctcccctgta ggcaagccaa tcggcacggt
      961 tctcctgcat tcggtgagcg ccgagcgcac cgatgtaacc ggcttcgctc tccagggcgg
     1021 ccatcagtgc ggcgccggcc agatcgaggt cgtgtgcggt gaccaccacc ttgtcgagcg
     1081 gggacagggt ggcgatcacc cccgttgccg aagcggcgtc gttgaacatc cgggccttcc
     1141 agccgaccaa tgacgccagt tcggcgaggg catctccgac cggggtgtcg ccaaccacca
     1201 cgagttgggg caccgggcgc agcacggtga tcacttggtc gtcgatcagt gtcgagccgc
     1261 tggcccctgc ctggaacaac tgctccacgt ctgggcctgc ttcgccaatg ttgtcgtccg
     1321 agaagaggct gatctcggcc acctcatcgc cttcgatccg gcacacaagg caaagaggtt
     1381 gtcgggcagc ggccatttcc cagagctcgc ccggaaaggc ggtggccggc accaccaggc
     1441 aacgagcaac gcccgctgtg ggaaggccgg cgatcagagc gtcgatctcg ctcacctcga
     1501 accggac
//
LOCUS       DBNHXU010000401         1791 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_765785, whole genome shotgun sequence.
ACCESSION   DBNHXU010000401 DBNHXU010000000
VERSION     DBNHXU010000401.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1791)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_765785"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="AC161X_09815"
     CDS             <1..495
                     /locus_tag="AC161X_09815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007027158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein DrrA1-3 family
                     domain-containing protein"
                     /protein_id="HIR4989893.1"
                     /translation="KGNRQKVALIAALASDVELLILDEPTAGLDPLMEAEFRACVNED
                     RHQGRTVLLASHILAEVEALCDRVSIVRAGKTVESGTLTELRHLTRTTISAEVANEPT
                     GLADLPGVHDLEVEDHRVHFEVDSEQLGPVLSHLTGLGVRSLTSQPPTLEELFMRHYR
                     DEIA"
     gene            492..>1791
                     /locus_tag="AC161X_09820"
     CDS             492..>1791
                     /locus_tag="AC161X_09820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989894.1"
                     /translation="MTTFTGTWHLIRFILRRDRFRLLVWILVLSSLAVGTASAFISLY
                     PNEAERQMLVKTIGSNPAIVAFLGPIYGSSVGNLTVWRVGVIGSVLIGVMAALTMIRH
                     TREEEETGRRELLGATVVGRNAPLTASLIVTASAGLILGALCTAGLIGLGLPTPGSIA
                     FGMGYAAIAIVFAGLSGVAAQLTQGAGAAKGIAIGMVGASFVLRLAGDGGSESGLGWM
                     SWLSPIGWFTQLRPYAEERWPVLLLSAGLALLATAGAFWLASRRDVGAGIVQPRLGAG
                     SASPRLGSPLGLAWRLHRPALLGWGLGLAFLGLVYGGIADSVGGFLTDSPQLAEIFEQ
                     LGGEAGITAAYFATAMGILAIIASAYAIRTALRARVEEESLRADSVLATATPRIRWAN
                     SHFIFAILGPVLILAVAGLVAGAVYGVIIGDVGGQVPRVLE"
BASE COUNT          277 a          573 c          595 g          346 t
ORIGIN      
        1 aaggggaacc gccagaaggt cgccctgatc gcggccctgg cttcggacgt ggagttgctc
       61 atcctcgacg aaccgaccgc aggattggat ccgctcatgg aggcggaatt ccgggcctgt
      121 gtcaacgaag acaggcacca aggcaggaca gtgctcctgg cgagtcacat cctcgccgag
      181 gtcgaggcgc tttgtgatcg ggtgagcatc gtgcgagcgg ggaagaccgt ggaaagtggg
      241 acgctcaccg aactccgcca tctgacccgg accacgatct cggccgaagt ggccaatgag
      301 ccgaccggac tcgccgacct ccccggggtc cacgacttgg aggtcgaaga ccaccgggtc
      361 catttcgaag tcgactccga gcagctcggg cccgtcctca gtcatctgac ggggctcggg
      421 gtgcgaagcc tgaccagtca gccgcccaca ctcgaagagt tgttcatgcg tcactaccgg
      481 gacgagatcg cgtgaccacc ttcaccggca cctggcatct gattcgattc atcctgagac
      541 gtgaccgctt ccgcctcttg gtctggattc tcgtgctgtc ctccctggcg gtaggcaccg
      601 ctagcgcctt catcagcctc tacccgaacg aggccgaacg tcagatgctg gtcaagacca
      661 tcggctcgaa cccggcgatc gtcgcctttc tcggcccgat atatggctcg tccgtcggca
      721 acctcaccgt ctggcgggtg ggagtcatcg gatccgtcct gatcggggtc atggctgccc
      781 tgaccatgat caggcacacc agggaggaag aggagaccgg gcgacgcgag ttgctggggg
      841 caacggtggt gggacgcaac gccccactga ccgccagcct catcgttacc gcatctgccg
      901 gcctgatcct gggggctctg tgcaccgcgg ggctcatcgg cctcggtttg ccgacaccgg
      961 gatcgattgc atttggcatg ggatacgccg ctatcgccat cgtcttcgcc gggctgtccg
     1021 gtgttgcggc gcagttgacc cagggtgccg gggccgcgaa gggaatcgcc attgggatgg
     1081 ttggtgcttc cttcgtgctc cgcctggccg gtgacggtgg ctccgaaagc ggtctgggat
     1141 ggatgtcgtg gctgtccccc atcggctggt tcacccagct gcggccctac gccgaggagc
     1201 ggtggcccgt gttgctgctc tccgccgggc tggcgctgtt ggcgactgcc ggcgccttct
     1261 ggttggcgtc gcgccgggat gtcggagccg ggattgtcca accgagactg ggcgccggct
     1321 cggcctcgcc acggctggga tctccgctcg gcctggcctg gagactgcat cgccccgccc
     1381 tgcttggctg gggcctcggt ctcgccttcc tcggtttggt atatgggggt attgccgaca
     1441 gtgtcggtgg cttcctcacg gacagtccac aactcgccga gatctttgag cagttgggtg
     1501 gcgaagccgg gatcaccgct gcctacttcg cgaccgccat ggggattctt gcaatcattg
     1561 ccagcgctta cgcaatcagg acagccctcc gcgcgagagt cgaggaggaa agcctccgcg
     1621 ccgactcggt gctggccacg gccacgcccc ggattcgctg ggcgaatagc cacttcatct
     1681 tcgcgatcct gggcccggtc ttgatccttg cggtggccgg attggtcgca ggcgccgttt
     1741 acggagtgat catcggcgat gtcggagggc aagtgccacg cgtgctcgag g
//
LOCUS       DBNHXU010000402         3146 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1361767, whole genome shotgun sequence.
ACCESSION   DBNHXU010000402 DBNHXU010000000
VERSION     DBNHXU010000402.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1361767"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            398..1216
                     /locus_tag="AC161X_09825"
     CDS             398..1216
                     /locus_tag="AC161X_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014625.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HIR4989895.1"
                     /translation="MPGLLSFHAHPDDESISTGGTLARYAANGEPVLVVTATDGALGE
                     IHNHEDPESIRPRLAQVRADEMAAALEILGVRDHAFLGYRDSGMMGWEDNFHPDCFWQ
                     ADFMEATRRLVHLIRKHRPEVVTIYDPFGGYGHPDHIQVHRVGIAAFHGSADLGRFPI
                     RGGEEPWQADRLWMSVWPRSRMAIWADIDEAAGTIDANEAARRRNSGFPDERVNVWMD
                     VEDLIEVKVAAIAAHVTQIPPDWALLNVPEAHKPTLLGHEAFVELYPRAGLGAS"
     gene            complement(1202..2284)
                     /locus_tag="AC161X_09830"
     CDS             complement(1202..2284)
                     /locus_tag="AC161X_09830"
                     /inference="COORDINATES: protein motif:HMM:NF014325.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium channel family protein"
                     /protein_id="HIR4989896.1"
                     /translation="MRARLRSLFDSPFLRRVNWHVKRVAEKIDRRFFASLLAGLAVFL
                     SLAATSIWLAETDRSIGQLGHSFYWAGATVLGLGEGGFVGGPVGWVVGWVLALFGVAI
                     VATITGALVGFVIDFLLKEGQGMGASGYRDHIVICGWNSTARDLISELAKDEYGTKIV
                     LLHDSERSPAPDQIYFVRGSTTNDSDLRRAGIEHAASAIVCPVDGSDQADMSSILTVL
                     AIESISPDVRTVVEVNNPDHVTHFERANVDEVMVTSKLASHLLARSAMYPGLSKLVTD
                     IVSGGEGSELYRVELPEDYAGLTVDDLSAKLRADHRATLLAVNRAGTSHPNPETGFLV
                     QAGDDLVVVAESLGEMLPLQEALARA"
     gene            2382..>3146
                     /locus_tag="AC161X_09835"
     CDS             2382..>3146
                     /locus_tag="AC161X_09835"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4989897.1"
                     /translation="MLIARDLRIEAGPRTLIDEVSFSVQSGDRIGLVGRNGAGKTTLM
                     RTLAGQLNAAAGSVLRSGTIGYFSQEAALPELEHQDATALERILMARDIGAMQRRIEE
                     ARHRLHDLTGEERDRGIVRFSRLHDEFEAKGGFVAEAEAKRMAAALGIGNDELGQPVV
                     TMSGGQRRRVELARILFADTDILLLDEPTNHLDLDAKAWLIEWLTTYRGGMLVVSHDL
                     PLLDEAITSVLALEHGELEAYRGNYSHYLLERDRRRE"
BASE COUNT          593 a         1015 c          973 g          565 t
ORIGIN      
        1 agctctgcct gcgggtcctc ccaatcggag tcgaaggccc cgaagctccc cgcggttatg
       61 accaggtcga tcgatgaatc cgggctctcg ccgaagacct cgtaggcgat gcgatccgag
      121 ccaagacgtg catacccggt cgtgcgctcc accaggcgag gatagctgcg gcctacctgc
      181 cactccgagc cggtatctcc tggttcttca acgaattcgg ggcgacgctg gcgtcgcccc
      241 gagttgcccc tgggaggagg gatctgttgt agtcacgatc cttcagaccg tgaccctcca
      301 atttgtaacc gggcgaggtg accggaaggt gaacgcaggc tggcgctcgc ccgaatcttg
      361 agtgaaggcc ggatttgggc gcccgtagac tcgatcgatg cccggcctcc tctcatttca
      421 cgcccatccc gacgacgaat cgatctccac cggggggacc ctggcccggt acgcggcaaa
      481 cggggagccg gtgctggtgg ttaccgctac cgatggcgcc ttgggtgaga tccacaacca
      541 cgaggatccg gagtcgattc gcccccgctt ggcccaggtt cgggccgatg agatggccgc
      601 ggcccttgag atcctcgggg tccgtgacca tgccttcctc ggttaccggg actccgggat
      661 gatgggctgg gaggacaact tccatcctga ttgcttttgg caggccgact tcatggaggc
      721 cacccggcgc ctggttcacc tcatcaggaa gcaccggccg gaagtcgtca ccatttacga
      781 cccattcggc gggtacgggc acccggacca catccaggtt caccgggtcg ggatcgccgc
      841 cttccacggc tcagccgatc tcgggcgatt tccgattcga ggcggggagg agccatggca
      901 agccgatcga ctgtggatgt cggtttggcc aaggagccgg atggcgatct gggccgacat
      961 cgacgaggcc gccggaacaa tcgatgccaa cgaagccgcc cgtcgccgca attcggggtt
     1021 ccccgacgag agggtcaatg tctggatgga tgtcgaggat ctgatcgagg tcaaggtcgc
     1081 cgccatcgcc gcccacgtga cccagatccc gcccgactgg gccttgctca atgtccccga
     1141 agctcacaag ccgacccttc tcggtcatga ggcattcgtg gagctatacc cccgcgccgg
     1201 gttaggcgcg agctaaggcc tcttgcaggg gaagcatctc gcccaacgac tctgcgacta
     1261 ccaccaggtc gtctccggcc tgaacgagga atccggtttc cggattgggg tgtgatgtgc
     1321 cggctctgtt gacagcaagg agggtggcgc ggtgatcggc gcgcaacttc gccgacaggt
     1381 catcgacggt gagccctgcg tagtcttcgg gaagctcgac cctgtacaac tccgatccct
     1441 caccgccgga cacaatgtcg gtcacgagct tcgacagacc cgggtacatg gccgaacgtg
     1501 ccagcaagtg cgatgcgagc tttgaggtga ccattacctc gtcgacgttg gcccgctcga
     1561 aatgcgtcac gtggtcggga ttgttgacct cgaccacagt ccgtacgtcc ggcgagatcg
     1621 actcgatggc gaggacggtg aggatcgagg acatgtctgc ctggtcggac ccgtcgacgg
     1681 ggcacacgat ggccgatgcc gcgtgttcga tgccggctcg gcgcaggtcc gagtcgttag
     1741 tggtgctccc gcgaacgaag tagatctggt ccggagcggg gcttcgctct gagtcatgaa
     1801 gcagcacgat cttggtgccg tactcgtctt tcgccagttc actaatcagg tctctcgccg
     1861 ttgagttcca accgcagatc acgatgtggt cgcgataccc ggacgcaccc atcccttgcc
     1921 cttctttcaa taggaagtcg atcacgaatc caaccagtgc cccggtgatc gtggcgacaa
     1981 tcgccactcc aaacaatgca agcacccatc ccacgaccca cccgaccggc cccccgacaa
     2041 accccccctc acccagaccg aggacggtgg ctccggccca gtaaaacgag tggccgagtt
     2101 ggccgatcga gcggtcggtc tctgcaagcc aaatggaagt ggccgccaag ctgaggaaca
     2161 cagccagacc tgccaacaac gacgcgaaga agcgtctatc gatcttctcg gccactcgct
     2221 tcacgtgcca gttcactcgg cgcagaaatg gcgagtcgaa cagggaccgc agtctggcgc
     2281 gcatgatcgc gaatttagtc gccgctcggg cccatccagc ttggtaaatc ggtcagattg
     2341 ggcgcaatcc gcacctaacg tctcgggcac ctggaacacc catgctgatt gcacgcgacc
     2401 ttcgcatcga agccggacct cgaactctca tcgacgaggt ctctttcagt gtgcaatcag
     2461 gagatcgcat cggcctcgtg gggcgcaatg gggccggcaa gaccacgttg atgaggacac
     2521 tcgccggcca gctcaacgct gcggcaggat cggtcctccg ttccggaacc atcggctact
     2581 tctcccagga ggcggccctt cccgaattgg agcatcagga cgccaccgct ctcgagcgaa
     2641 tcctgatggc gcgcgacatc ggggccatgc agcgacggat cgaagaggcc cggcaccgac
     2701 ttcacgacct caccggcgag gagcgtgacc gcgggatcgt ccgtttcagc cgccttcacg
     2761 atgagttcga agccaagggc ggattcgtgg ccgaggctga agccaagcgg atggcggccg
     2821 ccctgggaat cgggaacgac gaattgggcc aacccgtggt gacgatgtcg ggtggccagc
     2881 ggagacgcgt ggagctggcc cggattctgt ttgccgacac cgacatcctc ctgctcgacg
     2941 agccgaccaa ccacctcgat ctggatgcca aggcctggct gatcgagtgg ctgaccacct
     3001 atcggggcgg gatgctcgtg gtctcccacg acctgcccct tctcgatgag gcgatcacgt
     3061 ccgttctcgc tctcgagcac ggcgagttgg aggcctaccg gggcaactac tcccactatc
     3121 tcctggagcg ggaccgccga cgtgaa
//
LOCUS       DBNHXU010000403         4085 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2154130, whole genome shotgun sequence.
ACCESSION   DBNHXU010000403 DBNHXU010000000
VERSION     DBNHXU010000403.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4085
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2154130"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="AC161X_09840"
     CDS             <1..460
                     /locus_tag="AC161X_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989898.1"
                     /translation="IAARIRPNRTQPGFILGLVVVVVGGLALVSHMTNQVARDYLAIP
                     LGVFGGLTLGAIIWRMRSKWSSRRVEVAAAIYIVAFVTVSFSLDWFDPRGVVPSAILA
                     MIALLRRTTKDLDRPPEADPISANAAVVFLMVAAIVLLLLGYAPSGGDQL"
     gene            complement(469..1122)
                     /locus_tag="AC161X_09845"
     CDS             complement(469..1122)
                     /locus_tag="AC161X_09845"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017621179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HIR4989899.1"
                     /translation="MPRLVDLSHPIEAGMTTYPGMPGPVISDYMSRADSRGRYDPGTE
                     FNIGRIEMIANTGTYLDTPFHRFENGFDLADLPLEKVADLPGICVHNPGPELGAEVLY
                     DIDVSGRAVLFATGWDRHWRTEAYGDPSHPFVSAGLVDALVAGGAALVGIDSVNIDDT
                     RGGGRPAHTGLLEAGIPIVEHLTGLDRLVDQEFRFFSVPSPVRGMGSFPVRAFAVVG"
     gene            1157..1822
                     /locus_tag="AC161X_09850"
     CDS             1157..1822
                     /locus_tag="AC161X_09850"
                     /inference="COORDINATES: protein motif:HMM:NF014090.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005384 - manganese ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0030026 - intracellular manganese ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VIT1/CCC1 transporter family protein"
                     /protein_id="HIR4989900.1"
                     /translation="MTGLTPSFFRGRLVDANDGIIATAGIVEGLAAAGATGFTMVVAA
                     FAATVAGSVALAGAKFAETSLEREARERRIEEERRRVALSPEQEQSQLAAHYESRGLS
                     PNLAATVAAELSRNDPLGAQIDIEPDLGPDEGPSPLPAALWAGLAFALGSGIPLTAIL
                     VTPPSQRIPVTIVVVIASLVVTSIVTARAVGGNLRRALVRATCIGMVTMLVSLGAGLL
                     FRP"
     gene            complement(1842..3644)
                     /locus_tag="AC161X_09855"
     CDS             complement(1842..3644)
                     /locus_tag="AC161X_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011970120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HIR4989901.1"
                     /translation="MSDAAIVYLVLAAVVVLFIWNKFPVEVVAVGSALVLAATNVLTL
                     DQALAGFGDSTVLFIAALFVVSEGIDSTGVTTWAGQQLIERAGGSRTRLIVFMMLLVA
                     LLTAVISVNGAVAALLPMVVVVALRMGRAPSQLLMPLAFGAHAGSLLALTGTPVHILV
                     SDAAVQAGESGFSYFEFGLVGVPVLVGAIAIVVFLGSRLLPERKAESIPPDLSDHART
                     LIDQYSLADWVARLEVRAGSPLIGKTLSSLDLSPYPSMALVGAQGERGVALDDQSPIG
                     EGFVITVKGESEDIYRFAAGSDLERKPGHTGGKLIDRRIGVVEVVVPPRSEMIGVPVF
                     PGMITESGDFVVLAIQRQGEDMDRSTTTLAVGDTMLLQGTWDDLDSRFDSERGVLVVD
                     SPSLVRRQAIALGPRAWWAIAILLAMVVLLATNAVPAVEAALLAAGAMIITRVVTIYQ
                     AYRSISWTTVILVAGMIPLSTAVTQSGAADDLAGLLIDVVGTSGPYFLLLGLFVLTAV
                     FGQLISNMATALIMIPIAVTAATDVGISVRPVLMSLTVAAAAAFLTPVATPVNLMVMG
                     PGAYKFGDYWKLGFPMLLLFMAAAVFLVPVFWPF"
     gene            complement(3652..>4085)
                     /locus_tag="AC161X_09860"
     CDS             complement(3652..>4085)
                     /locus_tag="AC161X_09860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020489654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR4989902.1"
                     /translation="LPRVAAVEMAHFLEQANIGGLALLVGFILVIVILDFILPGVVPK
                     WAIFAPVFIPIFARLGVAPQTVLAAYRVGDSPVNVITPLMVYLPFIVTIAQRYKKDAG
                     IGTVVALMIPFVIAILIAWILLFMIWFWLGLPLGPGYPIGT"
BASE COUNT          821 a         1330 c         1288 g          646 t
ORIGIN      
        1 gatcgcggcc cggatccggc ccaatcggac gcagccgggc ttcatcctcg gactggtggt
       61 tgtcgtggtc ggaggactgg ctctggtgtc acatatgacg aatcaggtgg cgagggacta
      121 tctggccatc ccgttggggg tcttcggcgg gctgaccctc ggcgcgatca tctggcgaat
      181 gcgttcgaaa tggtccagca gaagggtgga agtggcggcc gccatctaca tcgtcgcgtt
      241 tgtgactgtg agcttcagcc ttgattggtt cgatccccga ggggtcgtcc cgagcgccat
      301 cctcgccatg atcgccctgt tgcgccgtac gaccaaggac ttggaccgac cgcctgaggc
      361 cgatccaatc tcggccaatg ccgccgttgt cttcctgatg gtggccgcaa tcgtcttgct
      421 cttgctcggc tacgcaccgt cggggggaga tcagctctag acagagcctc accccacgac
      481 ggcgaaagca cggaccggga agctccccat cccccgaacc ggagacggca ccgagaagaa
      541 tcgaaactcc tgatcgacca ggcggtccaa tccggtgagg tgttccacga tcggaatccc
      601 cgcctcgagc aaaccggtgt gggccggtcg accaccaccc cgagtgtcgt cgatgttcac
      661 cgagtcgatt cctaccagcg cggctccgcc ggcaaccagg gcgtcgacca gcccggcgga
      721 gacaaacggg tgcgacggat cgccataggc ctcagtgcgc cagtgccggt cccacccggt
      781 ggcgaacagg accgctcggc cactcacatc gatgtcgtag agaacctccg ctccaagctc
      841 cggacccggg ttgtggacgc agattcccgg caggtcggcc accttttcca agggaagatc
      901 ggccaggtcg aacccgtttt cgaaacgatg gaacggggtg tcgagatagg tgccggtatt
      961 ggcaatcatc tcgatccgac cgatgttgaa ctcggtcccc gggtcgtacc gaccccgcga
     1021 gtccgcccgg gacatgtagt cgctaatcac cggccccggc attcctggat aagtggtcat
     1081 cccggcctct attgggtggg agaggtcaac gagacgcggc atgatgtcga ccacactacg
     1141 tccccgcatg aatctgatga caggcctgac cccgtcgttc tttcgggggc gattggtaga
     1201 cgccaacgac ggcatcatcg ccacggctgg aatcgtcgaa gggttggctg ctgccggggc
     1261 aaccgggttc accatggtcg tggcggcatt cgccgccacg gtggccggga gtgtggcttt
     1321 ggccggagcc aaatttgccg agacttcgct ggagagggaa gcgcgcgagc gccgaatcga
     1381 ggaggagcgt cgccgggtgg ccctgtcgcc cgagcaggag caatcccagc tcgccgccca
     1441 ctatgagagc cggggattga gcccgaatct ggctgccacc gtggccgccg agttgagccg
     1501 caacgatccg ctcggcgccc agatcgacat cgagcccgac ctcggcccag acgagggacc
     1561 gagcccactt cctgccgccc tctgggcagg tctggccttc gctctcggct ccgggatacc
     1621 gctcacggcg atcctggtga ccccgccgag ccagcgcatt ccggtcacga tcgtcgtggt
     1681 gattgcctcc ctcgtagtga cgtccatcgt caccgcccga gccgtcggcg gcaacctgag
     1741 acgggcgctg gtcagggcca cgtgcatcgg aatggtcacc atgctggtga gcctcggtgc
     1801 agggctcttg ttccgtccgt aagccgcttc gagcagctcg atcagaacgg ccagaacacg
     1861 gggaccagga acacggcggc cgccatgaac agcaggagca tggggaagcc caacttccag
     1921 taatcgccga atttgtaggc gcccggcccc atcaccatca gattgactgg tgtggccacc
     1981 ggggtgagga aagccgctgc cgccgccacc gtgagtgaca tgaggaccgg acgcaccgag
     2041 atcccgacat cggtggccgc tgtgaccgca attgggatca tgatcaacgc cgttgccatg
     2101 ttggagatca gctggccgaa gacggcagtc aggacaaaca gccccaagag gaggaagtag
     2161 ggccccgagg tacccacgac gtcgatcagg aggccggcca agtcgtcggc tgcgccgctc
     2221 tgggtcaccg cggtcgacag cgggatcatt cccgccacaa ggatcaccgt cgtccacgag
     2281 atggaccgat atgcctggta gatggtgacc acccgggtga tgatcatcgc ccccgcggcc
     2341 aggagcgcgg cctccacggc agggaccgcg ttggtggcga gcaggaccac catcgccaga
     2401 agaatggcta tggcccacca ggcacgtggc ccgagcgcaa tcgcctgacg ccgaaccaga
     2461 gacggagagt cgaccaccag cactccccgc tcggaatcga accgactgtc cagatcgtcc
     2521 caggtcccct ggagcaacat ggtgtcaccg acggcgagtg tcgtcgtcga ccgatccatg
     2581 tcctctcctt ggcgttggat ggcgagaacg acgaagtcgc cactctcggt gatcatcccg
     2641 gggaacacgg gaacaccaat catctcagag cgcggcggaa ccacgacctc gaccaccccg
     2701 atcctgcgat cgatcagctt ccctccggtg tggcctggct ttcgctccag atcgctcccg
     2761 gcagcgaacc gatagatgtc ctccgactcc cctttgaccg tgatcacgaa tccctccccg
     2821 atcggcgact ggtcatcgag ggcgacgccg cgttcaccct gcgcccccac gagagccata
     2881 ctcggataag ggctcaggtc gagcgaggac agcgtcttgc cgatgagggg tgagcccgct
     2941 ctgacctcga ggcgggccac ccagtccgcg agcgagtact ggtcgatcag ggtgcgggca
     3001 tggtcggaga gatcaggtgg aatcgattcc gccttgcgct ccgggagcaa gcgggacccc
     3061 aagaacacga cgatcgcaat cgctcccacc agtaccggca cgccgaccag gccaaactcg
     3121 aagtagctga agccgctctc cccggcctgg accgcggcgt cggagaccag gatgtggacc
     3181 ggggtcccgg tcaaagcaag caaggagccg gcgtgggcgc cgaaggccag gggcatgagc
     3241 agttgtgacg gtgcccgacc catacgcagg gcaaccacca ccaccatcgg gagtagagcc
     3301 gcaactgccc cgttgacact gatcactgcg gtgagcagag ccaccagcag catcatgaaa
     3361 acgatcaacc tcgtacggct accacccgcg cgctcgatca gttgctgccc cgcccatgtg
     3421 gtcacacccg tggaatcgat cccctcgctg actacgaata gggcagcgat gaagagaacc
     3481 gttgagtcgc caaacccagc gagggcctgg tcgagcgtga gcacattggt cgccgccagc
     3541 accagagccg agccgaccgc caccacctcg accgggaact tgttccagat gaagaggaca
     3601 acgacagccg caagaaccag atagacgatg gcggcgtcgg acattgggag gctaggtacc
     3661 tatcgggtac cccggtccca gcggtagacc gagccagaac cagatcatga acaggaggat
     3721 ccaggcaatc aggatggcga tcacaaacgg aatcatcagc gccacgacgg ttccgatgcc
     3781 agcgtccttc ttgtacctct gtgcgatggt cacgatgaac ggcagataga ccatcagcgg
     3841 cgtgatcacg ttcaccggtg agtcgcccac cctgtacgcg gcgagcacgg tctggggcgc
     3901 cacaccgagc cgggcaaaga tcgggatgaa caccggagcg aagatcgccc atttcggtac
     3961 caccccgggg aggatgaagt cgaggatcac tatcacgagg atgaagccga ccaacaacgc
     4021 cagcccaccg atgttggcct gctcgaggaa gtgcgccatc tcgacggcgg cgacccgggg
     4081 caggt
//
LOCUS       DBNHXU010000404         3270 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4735333, whole genome shotgun sequence.
ACCESSION   DBNHXU010000404 DBNHXU010000000
VERSION     DBNHXU010000404.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4735333"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1059)
                     /gene="ychF"
                     /locus_tag="AC161X_09865"
     CDS             complement(<1..1059)
                     /gene="ychF"
                     /locus_tag="AC161X_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019058470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HIR4989903.1"
                     /translation="MSLTIGIVGLPNVGKSTLFNALTDARILAANYPFATIEPNTGVV
                     PIPDPRLQELASLFDSKKVTPATVTFVDIAGLVRGASEGEGLGNQFLANIRDVNAICH
                     VVRVFDSESVVRVGEGGSPASDIETIETELGLADLQTIEKRLPRLEREATLDRSLAPQ
                     VELLRALQEKVGSGEMVSSDRSLMVRQHEFADLHLLTAKPIIYVFNCDPGLLTDADGR
                     SALVATVAPAEALFLDAQIEAELNELDEEDRVEMLAAVGQEEPGLNSVIRSAYRTLGL
                     QSYLTAGEKEARAWTIRSGATAPEAAGVIHTDFEHGFIAAEVIDFDQLVRAGSWVEAR
                     SKGMVRTEGKSYVMRPDDV"
     gene            1110..2108
                     /locus_tag="AC161X_09870"
     CDS             1110..2108
                     /locus_tag="AC161X_09870"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012773722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase (FAD) complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HIR4989904.1"
                     /translation="MDRIAVVGAGSWGTTAAALAARSTPTVLWARRPELAKEITETGE
                     NRQYLPGLALPEGLTTTSDLGAAVDGASAIVMAIPSHGFREIYSQIANVVAEEIPVLS
                     LTKGIEQETLATMTQVVREASPGKPGSLVGVMTGPNLAGEVAAGQPTASVIAMADQAT
                     ARGLQDVFMGPTFRVYTHDDVVGCELGGALKNVMAIAAGMSDGLGFGDNTRASLITRA
                     LAELTRLGVALGGRPATFAGLAGMGDLIATCISTASRNHQVGAALASGKTLTEVEAEM
                     QMVAEGVKTTRAVLDLAGRQGIEMPIASHVGQVLYDGLQPREAVLRLMTREAKQED"
     gene            2110..2748
                     /locus_tag="AC161X_09875"
     CDS             2110..2748
                     /locus_tag="AC161X_09875"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003988828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HIR4989905.1"
                     /translation="MSSKKTVIRRLAELAVHDMSNIHAILDEGFVCHVAYLNDGRPVV
                     IPTLYARDGDRVLLHGSHASGLMLAVKRGSPLSIAVTLVDGLVVARSAFESSANYRSV
                     VIHGHGRFLEGEEHEAALDVIVEAVIPGRLADIRRPTTQELRKTAIFEVGLDEVSAKV
                     HVGPPDDAESDLGTGVWAGVIPLQLTVGTAEPAPDLEPGVELPDYLKPYRRS"
     gene            2807..>3270
                     /locus_tag="AC161X_09880"
     CDS             2807..>3270
                     /locus_tag="AC161X_09880"
                     /inference="COORDINATES: protein motif:HMM:NF013088.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HIR4989906.1"
                     /translation="MANQILTTSHGTNREAFSPRDWGLFLGISAIWGSSFLWMAIGLD
                     AFHPGLVTWLRIGFGAAFMAALPQARRAQIPRMERKRIAVLGLIWLAIPFVIFPIAQQ
                     WIDSSVTGMLNGATPIFTALVASILLWQLPGRLQFIGLVVGFSGILAIALPSA"
BASE COUNT          567 a         1052 c         1035 g          616 t
ORIGIN      
        1 gacgtcgtcc gggcgcatca catacgactt gccctcggtg cgaaccatgc cttttgacct
       61 ggcctcgacc cacgaacctg cccggaccag ctggtcgaag tcgatcacct ctgccgcgat
      121 gaatccgtgc tcgaaatcgg tgtggatgac tccggcagct tcgggggcgg tcgctccgga
      181 tctgattgtc caggctcgcg cctctttctc ccccgccgtc agatagctct gcaagcccaa
      241 tgtccgatag gcggacctga taaccgagtt cagccccggc tcttcctgtc cgacggctgc
      301 gagcatctcg accctgtctt cttcgtcgag ctcgttcaac tcggcctcga tctgggcgtc
      361 gaggaagagt gcctcggcag gggcgaccgt tgccacaagc gccgacctcc cgtcggcgtc
      421 ggtaagaagg cccggatcgc agttgaagac atagatgatc ggcttggcgg tgagcaagtg
      481 gaggtcggca aactcgtgct gacggaccat caggctccga tcgctgctca ccatttcccc
      541 cgagccaacc ttttcctgca gggcccgaag caactcgacc tgaggtgcca gtgaccggtc
      601 cagggttgcc tctctttcca gccgcggaag acgcttctcg attgtctgca ggtcggcgag
      661 acccaactcg gtctcgatgg tctcgatgtc cgatgccggg gaaccgcctt cgcccaccct
      721 gacgacactc tcggaatcaa acacccggac cacgtggcag atcgcgttga cgtcacggat
      781 gtttgccagg aactgattgc ccaggccttc gccctcgcta gcacctcgaa ccaacccggc
      841 gatgtcgacg aaagtcactg tcgccggagt gaccttcttc gagtcgaaca ggctcgccag
      901 ctcctgaagt cgagggtcgg gaatgggcac aacgccggtg ttgggctcaa tggtggcgaa
      961 cggatagttg gcggcgagga tcctggcgtc ggttagcgcg ttgaagagtg tcgactttcc
     1021 gacgttgggg aggcccacga tgccgatggt caggctcatg ctgggcgcag gttaggaagg
     1081 gcatggcggc gactggctag cgtgccgaca tggacaggat cgcagttgtc ggcgccggct
     1141 catgggggac gaccgccgcc gccctggccg cccgctcgac gccaacggtg ctctgggcaa
     1201 ggcgcccgga actggcgaaa gagatcaccg aaacaggcga gaaccgtcaa tatctgccag
     1261 ggctcgccct gcccgaaggt ctgaccacca cctcagatct cggggccgcg gtcgatggcg
     1321 cttcggccat cgtcatggcc atcccgtccc atgggttcag ggagatctac agccaaatcg
     1381 ccaatgtcgt ggctgaagag atcccggtgc tctccctgac caagggcatc gagcaggaga
     1441 ccctggcgac tatgacgcag gttgttaggg aagcttcgcc gggaaagccg ggctcgctgg
     1501 tcggggtgat gaccggaccc aatctcgccg gcgaagtcgc cgccggtcag ccgaccgcga
     1561 gtgtcatcgc tatggccgac caggcaacag cgcgcggcct tcaggatgtc ttcatgggac
     1621 ccacattcag ggtctacacc cacgacgatg tggtgggctg tgagctgggc ggcgccttga
     1681 agaacgtgat ggcaatcgca gccggcatgt ctgatggcct cggattcggt gacaacacga
     1741 gagcttcact gatcacccgg gccctcgcgg agctcacccg cctcggcgtg gccctcggcg
     1801 ggcgtccggc cacctttgcc ggcctggcgg ggatgggcga cttgatcgca acctgcatct
     1861 caaccgccag ccgcaaccac caggttggtg ccgccctcgc atcggggaag acgctcactg
     1921 aagtcgaagc cgagatgcaa atggtggctg aaggcgtgaa aacgaccagg gccgtgctcg
     1981 atctggccgg tcgacaaggg atagagatgc ccatcgccag tcacgtcggc caggtcctct
     2041 acgacggcct tcagccgcgg gaggcggtgc tccgcctgat gacccgcgag gcgaagcagg
     2101 aggattgaca tgtcatcgaa gaagaccgtg attcggaggc tcgccgagct cgccgttcac
     2161 gacatgagca acatccatgc catcctcgac gagggattcg tctgtcatgt tgcctacctg
     2221 aatgacgggc gccccgtggt catcccgact ctttacgccc gtgatggtga ccgggtcctc
     2281 ctccacggat cccacgcctc gggcttgatg cttgcggtca aaaggggctc gcctctgagc
     2341 attgcggtca cgctggtgga cggactggtg gtggcccgca gtgcattcga gtcctcggcc
     2401 aactatcgca gcgtcgtcat ccacggtcac ggccgcttcc tcgaaggcga ggagcatgag
     2461 gcagcgctcg acgtgatcgt ggaggcggtg atcccgggtc ggttggccga catcaggcgt
     2521 cccacgaccc aggagctgcg caagacggcg atcttcgagg tcggcctcga tgaggtgtcg
     2581 gccaaggtgc atgtcggccc ccccgatgat gccgagtcgg acctgggtac cggtgtctgg
     2641 gcgggtgtca ttcctctcca gttgacggtg ggcacggccg agccggcgcc ggacctcgag
     2701 ccaggtgtcg agctcccgga ctatctgaag ccgtaccggc gttcctagat cctgcaaccg
     2761 ctgaggcggt gctggtgttc ttgacagaga tgagaaacga gcacgtgtgg ccaaccagat
     2821 cctgaccacc tcccacggca ccaaccggga ggcattcagc cccagggact ggggcctttt
     2881 cttgggtatt tccgcgatct ggggatcgtc attcctctgg atggcgatcg ggctcgacgc
     2941 gtttcacccc ggtctcgtca cctggctccg gattggcttc ggcgccgcct ttatggcagc
     3001 actcccgcaa gcccgcaggg cccagattcc caggatggag cgaaagcgga tcgcggtcct
     3061 cgggctgatt tggcttgcca tacccttcgt gatcttcccc atcgcccagc agtggatcga
     3121 ctcgtccgtc actgggatgt tgaacggcgc cactccgatc ttcaccgctc tggtcgcctc
     3181 gatcctgctc tggcagctcc ctgggcggct ccaattcatc ggcctggtgg ttgggttcag
     3241 cggcatcctc gccatcgccc tcccctccgc
//
LOCUS       DBNHXU010000405         2435 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3943440, whole genome shotgun sequence.
ACCESSION   DBNHXU010000405 DBNHXU010000000
VERSION     DBNHXU010000405.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2435)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2435
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3943440"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1211
                     /locus_tag="AC161X_09885"
     CDS             <1..1211
                     /locus_tag="AC161X_09885"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015909396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HIR4989907.1"
                     /translation="PVANAIVWQDTRTADICSRLAEGEGADRFRPVTGLPLATYFSGP
                     KIRWLLDEHNLHDAAARGELAFGTIDSWLAWTLTGGPGRGAHITDVTNASRTLLMDLA
                     TTTWDADIAVAMEIPTVMLPEIMPSIGVLGSCIGPLDGVPLTAILGDQHAALFGQVCF
                     RPGEAKNTYGTGNFMLLNTGPTPVVSEAGLLTTLAYQRSGEKPVYALEGSVAVTGSLV
                     QWLRDNLGLVGDASEIEKLATTVEDNGDVYFVPAFSGLFAPYWRPDARGVITGLTRYA
                     NRGHIARAALESTAYQTRDILDAMTSDSGVRLTELRVDGGMTSNSLLMQFQADILGVE
                     VIKPRVAETTALGAAFAAGLAVGFWANLDEISDLWEEGSRWQPQMSESDRERFYARWQ
                     EAVERSLGWV"
     gene            1281..1823
                     /gene="pgsA"
                     /locus_tag="AC161X_09890"
     CDS             1281..1823
                     /gene="pgsA"
                     /locus_tag="AC161X_09890"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR00560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HIR4989908.1"
                     /translation="MLALSRILLVPVIMAFTRSEQIPHSLAIAAWLFALAAFTDYLDG
                     YFARKISGGTVLGAFLDTTADKILVTGTLLALVSIDRVSVWPAALIIFREFGVMALRG
                     VVAAQGGLIRPSTWGKLKTATQFAALFLAYFRLAEPWGPWYLDQWAMLVAVVATIGSA
                     WGYTTAFWHTVRAEPAGTRR"
     gene            1820..>2435
                     /locus_tag="AC161X_09895"
     CDS             1820..>2435
                     /locus_tag="AC161X_09895"
                     /inference="COORDINATES: protein motif:HMM:NF013530.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HIR4989909.1"
                     /translation="MKVAVTGGSGVVGAAVIRHLVEAGHRVRALARSGEASIKVSAIG
                     AEAVPGDVLDPKSLANLVSGAERVFHVAGVNDMCSMDPGNMDRVNIVGTSNIRDACLV
                     GGVRRLIHTSSAVTIGEKQGSVGSETSPHRGSYLSRYERSKHISELLVLEARAGLEVV
                     SVNPSSVQGPGRATGTAKLILDVIRGKLPFLLGTTVSIVDIDDCA"
BASE COUNT          405 a          767 c          779 g          484 t
ORIGIN      
        1 ccccggttgc gaatgccatc gtgtggcagg acaccaggac cgcggacatc tgctcacgcc
       61 tcgccgaagg tgagggagcc gatcgcttcc gcccggtcac cgggttgcct ttggcgacct
      121 atttctcggg gcccaagatc cgctggctcc tcgacgagca caaccttcat gatgcggcag
      181 cccgcggcga gctggccttc ggcaccatcg actcctggct ggcgtggacc ctgactggag
      241 ggccgggccg gggcgcccac atcaccgatg tcacgaacgc ctctcgaacc ttgctcatgg
      301 atctcgccac caccacctgg gatgcggaca tcgcggtggc tatggagatc ccgacagtca
      361 tgcttcccga gatcatgccg tcgatcgggg tgctcggcag ctgcatcggg ccgttggacg
      421 gggtgcctct gaccgccatt ctcggcgacc aacacgcggc acttttcgga caagtctgct
      481 tccggccggg ggaggcaaag aacacatacg gaaccggcaa tttcatgctg ctcaacactg
      541 gcccgactcc ggtggtctcc gaagcgggac tgctgacgac attggcctat cagcggtccg
      601 gcgagaagcc ggtgtatgcc ctggagggtt ccgtggcggt gacgggatcc ctggtgcaat
      661 ggctgcggga caacctcggt ctggtcggtg atgcctccga gatcgagaaa ttggccacca
      721 ccgtcgagga caacggggat gtctatttcg tccccgcctt ctccgggttg tttgccccgt
      781 actggcggcc agacgcccgg ggggtcatca ccggcctcac ccgatatgcc aatcgcggtc
      841 atatcgcccg tgccgctctc gagtcgaccg cctatcagac ccgcgacatc ctcgacgcga
      901 tgacatctga ttccggggtc cgtctcaccg agttgagggt ggatgggggg atgacgtcca
      961 actcgctact catgcaattc caggccgaca tcctcggggt ggaggtcatc aaaccccgag
     1021 tcgccgagac caccgcgctc ggcgctgctt tcgccgccgg tctcgccgtt ggattctggg
     1081 cgaatctcga cgagatctcc gatctctggg aagaaggctc gagatggcaa ccgcagatgt
     1141 ccgagagtga tcgggagcgg ttttacgccc gatggcagga agctgtggaa cgatcattgg
     1201 gatgggtatg acacggggtt tggctgccac ctatcgataa gatcgctgcg agtcgaggag
     1261 gtctcatctc tctgcctgac atgctcgcgc tgtccaggat cctgttggtg cctgtgatca
     1321 tggccttcac gaggagtgag cagattcccc actccctggc cattgccgcc tggcttttcg
     1381 ccctggccgc atttaccgac tatctcgacg gctatttcgc tcgaaagata agtgggggca
     1441 cggtcctcgg cgcctttctc gacacgaccg cggacaagat cctggtcacc gggaccctgc
     1501 tcgccctggt gtcgatcgac cgggtctcgg tgtggccggc cgcactgatc attttccgtg
     1561 agttcggtgt gatggccctg cgcggcgtgg tggcggcaca gggcggcctg atccgaccct
     1621 cgacctgggg caagctgaag acggcgacgc agttcgcggc cttgttcctg gcctatttca
     1681 gattggccga gccctggggc ccctggtacc tcgatcagtg ggcgatgttg gttgccgtcg
     1741 tcgccaccat cggttcggcg tggggctaca ccactgcttt ctggcacacg gtgcgcgccg
     1801 agcctgccgg caccaggcgg tgaaggtcgc tgtcaccggc ggctcaggtg tcgttggagc
     1861 ggccgtaatc aggcatctcg tcgaagccgg ccaccgggtg cgggcccttg cccgaagcgg
     1921 agaggcctca atcaaggtca gcgcaatcgg ggccgaggcg gttcccggtg atgttctcga
     1981 tccgaagagc ctcgcaaact tggtctctgg cgccgagcgc gtcttccacg tcgccggggt
     2041 caacgacatg tgctcgatgg acccggggaa catggatcgg gtcaacatcg ttggcacctc
     2101 caacattcgt gatgcctgtc tggttggggg agttcgacgc ttgatccaca cctcgtcggc
     2161 cgtgaccatc ggcgagaagc agggaagcgt tggctccgag accagcccgc accgtggcag
     2221 ctatctgtca cgttatgagc gatcgaagca catctcggaa ctgctggttc tggaagcaag
     2281 ggccggtctc gaagtcgtct cggtgaatcc cagttcggtc caaggtccgg gccgggcgac
     2341 cggcaccgca aagctgatcc tcgatgtgat ccgcgggaag cttccattcc tcctcggcac
     2401 cacggtttcg atcgtggaca tcgacgactg cgctc
//
LOCUS       DBNHXU010000406         4029 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4936476, whole genome shotgun sequence.
ACCESSION   DBNHXU010000406 DBNHXU010000000
VERSION     DBNHXU010000406.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4029)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4029)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4029
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4936476"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..417)
                     /locus_tag="AC161X_09900"
     CDS             complement(<1..417)
                     /locus_tag="AC161X_09900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail sheath family protein"
                     /protein_id="HIR4989910.1"
                     /translation="MTGGYTAPGIYIEEITLPQTIVGVGTGTTAFVGRAARGPVDTPT
                     LITSFLDFERVFGGLWLRSDLGYSVRDFFEQGGRRAIVVRAYLAAANAVATLTFGRGS
                     SRLILEAATPGAWGSSLEATIDPLPRNRFDLTVTDRG"
     gene            456..1280
                     /locus_tag="AC161X_09905"
     CDS             456..1280
                     /locus_tag="AC161X_09905"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020669342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HIR4989911.1"
                     /translation="MTTIPLGTFGFWRALRTTTADMAREAETLGYGAIWVGASPTGDL
                     VDVEVLLEATERIPVVTGIVNMWREPAELVAASYHRIAVLHSDRFLLGVGIGHREATA
                     EFQAPMAKIDDYLNLLGQAGVPRDRLVLAALGPKALQVAAERTAGAHPYLTTPDHTRF
                     ARGVMGPGPLLAPEHKVTLDNDPERARATGRKVVAYYLDRVNYRNNLIRSGWTEDDLA
                     DGGSDALLDALVLHGTPEQVAVGLRAHIDAGANHLGVQVLGEEPMPTYRRLAEVLF"
     gene            1367..1804
                     /locus_tag="AC161X_09910"
     CDS             1367..1804
                     /locus_tag="AC161X_09910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR4989912.1"
                     /translation="MDGRDVVDLYLKAAGSGDLDTVREMQHPDIVGRYPQSGEVFRGR
                     GNYMDMQVAYPGLPHAEASNVIGQDNVVLMPAFLPFGHPTVTVFGGDRFVVEGVATYP
                     NGEVYNIVLILRLQSGKVIEETVYFAAPFDAPEWRRLYADRQV"
     gene            complement(1813..2979)
                     /locus_tag="AC161X_09915"
     CDS             complement(1813..2979)
                     /locus_tag="AC161X_09915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012830605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HIR4989913.1"
                     /translation="MVTERIRQITEANETLLSFLPIWEEHRRQPDVADLAFGNPQEMV
                     VDGFASALASVTTPRDKGHYAYKFSEEESRRTVANNLAEWRGQQFDPEDIAMTPGAFG
                     AIAAALGALVEVGDEVIYCDPPWFFYRSMILASGATPVSVPVRSDDYDLDLDRIASVI
                     TEKTRLVIVNTPHNPTGRIYPPSTLQGLATVLTEASDRFGAPIFLLSDEPYSKLVFSN
                     AKFTSPAEYYPHTLVSYSYGKILLTPGQRIGWLALSPLMPDPEPVRSAIFMAQIAGGW
                     LFPSAIMQYSLEQLDKLSIDLVELERKRDFTVAELQAAGYDLQTPEGTFYLWVRSPDP
                     DDVAFVEMLAMQGVLVLPGTTCASPGHFRISLTGTGEMIERSLPAFREATSGGS"
     gene            3035..>4029
                     /locus_tag="AC161X_09920"
     CDS             3035..>4029
                     /locus_tag="AC161X_09920"
                     /inference="COORDINATES: protein motif:HMM:NF013252.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HIR4989914.1"
                     /translation="MSTSEPIPDVDPLTAPVGDTFVIQQHHATALHHDFRLEMLSEEG
                     PVLVSWAVPKGLPRRKGERHLAIRTPDHSMDHATFSGSIPGDEYGGGEVRIFDHGEYE
                     MVGRTDDRISFRLQGERLAGVWHLVSTGLKGGKEQWLALMSKDLRLAAQARPPLEPML
                     ATPTAEAFDNPEWGFEPKWDGIRALAVCEEETHLISRRNHDITVAYPELQRIHEQVVA
                     LDAVLDGELVAFDDGVPSFQTLQQRMHLRNLKHVEQLSLSLPVVYMVFDLLYLDGRDL
                     TDQPLQERRRMLEEAVVPSEHIQISPSVIGDGVALFRAAAGQGLEGIMAKRLSSL"
BASE COUNT          742 a         1252 c         1318 g          717 t
ORIGIN      
        1 tccccgatcg gtcacggtca ggtcgaaccg gttgcgggga aggggatcga tcgtcgcctc
       61 caaactcgag ccccacgctc cgggagtggc cgcctccagg atcaatcggg agctgcccct
      121 cccgaaggtc agagttgcga cggcgttggc agcggccagg taggcgcgga ccacgatggc
      181 ccttcgtccg ccctgctcga agaagtctcg gaccgagtac cccaggtcgc ttctgagcca
      241 gagcccgccg aacacacgct cgaagtcgag gaacgaggtg atcagggtgg gggtgtcgac
      301 cgggccccgg gcggcacggc ctacgaaggc ggtagtcccg gtgcccaccc cgacaatcgt
      361 ctggggaagg gttatctctt cgatgtagat cccgggtgcg gtgtagccgc cagtcatcgt
      421 cgaggacgct atcggtctgt gccaggatgc gggtcatgac caccattcct cttggaacgt
      481 ttggcttctg gcgagcactc cgaaccacga ccgcggacat ggcccgggag gcggagaccc
      541 tcggttacgg cgccatctgg gtgggggcgt cacccaccgg cgacctggtg gatgttgaag
      601 tccttctcga agccacggag cggatcccgg ttgtcaccgg gatcgtcaat atgtggcggg
      661 agcctgccga attggtcgcc gcctcatatc accggatcgc tgtactgcat tccgatcgat
      721 tcctgttggg ggtggggatc gggcaccggg aggccacggc ggagttccag gcgccgatgg
      781 ccaagatcga cgactacctg aatctcctcg gccaggcagg ggtgccccgg gaccggttgg
      841 ttctcgccgc cctcgggccg aaggcgctcc aagtcgccgc tgagcgcacc gcgggggccc
      901 acccctatct gaccaccccg gatcacaccc gcttcgctcg tggagtaatg ggccccggtc
      961 cattgctcgc cccggagcac aaagtcaccc tcgacaacga tccggagcgg gcccgggcga
     1021 ccgggcggaa ggtggtcgcc tattacctgg accgggtcaa ctaccgcaac aacttgatcc
     1081 gcagcggttg gacagaggac gacctggccg atggtggcag tgatgcgctg ctcgacgccc
     1141 tcgtgctcca cgggacgccg gagcaggtgg cggttgggct ccgggcccat atcgacgccg
     1201 gggcgaacca cctcggagtt caggtgctgg gggaggagcc aatgcccacg taccgtcgat
     1261 tggccgaagt cctgttctag aggccagaac ccaacgctca ccagacactg tcccctattt
     1321 gtcgatatct ttccggagtc ggagccggtc agatcgaagg agtgtcatgg acggtcgcga
     1381 tgtcgtggat ctctatctga aggcggctgg atccggggac ctcgacaccg ttcgtgagat
     1441 gcagcacccc gacatcgtgg gtcgataccc ccaatccggc gaggtctttc gggggcgagg
     1501 caattacatg gatatgcagg tcgcctaccc cggacttccc cacgcggagg cttcaaacgt
     1561 gatcggccag gacaacgttg tcctcatgcc cgcatttctg ccgttcgggc acccaacggt
     1621 cactgtcttc ggcggcgacc gattcgtggt ggagggggtt gccacctatc ccaacggcga
     1681 ggtttacaac atcgtgctca tccttcggct gcaaagcggg aaggtgattg aagagaccgt
     1741 ctacttcgcc gcgcccttcg acgccccgga gtggcgccgg ctatacgccg accgacaagt
     1801 ctgagtaacg cctcagctac cgccggaggt ggcctcccgg aaggcgggaa gactccgttc
     1861 gatcatctcc ccggttccgg ttagcgagat ccggaagtgt cccggcgagg cgcaggtggt
     1921 gcccgggagc accaagaccc cctgcatggc gagcatctct acgaaggcga catcgtcagg
     1981 gtcgggagag cggacccaca gatagaaggt cccctccggt gtctggaggt cgtaaccggc
     2041 cgcctggagc tcggcgaccg tgaagtctcg cttccgctcg agttcgacga gatcgatcga
     2101 aagcttgtcg agctgttcga gggagtactg catgatggcg ctcgggaaca gccatccgcc
     2161 cgcgatctgg gccatgaaga tcgccgatcg cactggttcg gggtcgggca tcagaggcga
     2221 cagggccagc catccgatcc gttgaccagg tgtgagcagg atcttgccgt agctgtagga
     2281 gaccagtgtg tgggggtagt actcagccgg gctggtgaac ttggcgttgc tgaacaccag
     2341 cttggaatag ggctcgtcgg agagcaggaa gatgggcgcc ccgaagcggt ccgaggcttc
     2401 ggtcaagacc gtggccaggc cctgaagcgt tgagggcggg tagatccgac cggtcgggtt
     2461 gtgtggggtg ttcacgatga ccagacgggt cttctcggtg atcaccgacg caatccgatc
     2521 caggtcgagg tcgtaatcgt cgctccgaac cggcacgctg accggggtcg caccggaagc
     2581 gaggatcatc gatcggtaga agaaccaggg aggatcgcag tagatcacct cgtccccgac
     2641 ctcgacgagc gcgccgagag cagcagcgat ggcgccgaaa gctcccgggg tcatggcgat
     2701 gtcctcgggg tcgaattgct ggccccgcca ctcggccaga ttgttggcca cggtgcgccg
     2761 agactcttcc tcggagaact tgtaggcgta gtggcccttg tcacgggggg tggtcaccga
     2821 ggcgagggcg gaggcgaacc cgtcgaccac catttcctga gggttaccga atgcaaggtc
     2881 ggcgacatcg ggctggcgac gatgctcctc ccatatcgga agaaacgaca acagcgtctc
     2941 gttggcttcc gtgatctgtc ggatccgctc agtcaccatc gccggcgacg atagccaccc
     3001 tccgataggg tcgacatttc ccctcaccca tgccatgagc acttcggagc ccatccccga
     3061 cgtcgatccg ctgactgcac cggtcggcga cacattcgta attcaacagc accacgccac
     3121 cgccctccat cacgactttc ggctcgaaat gcttagcgaa gaaggcccgg tgctggtctc
     3181 ctgggcggtc cccaaaggcc tgcctcggcg aaagggcgaa cgccacctcg ccatccggac
     3241 ccccgatcat tcgatggacc acgccacgtt ctcggggtcc atcccaggtg acgagtacgg
     3301 cggtggagag gtccggattt tcgaccacgg cgagtacgaa atggttggtc ggaccgatga
     3361 ccggatcagc ttcaggctcc aaggggagcg gctggccggg gtctggcatc tggtgtcgac
     3421 cggtctcaaa ggagggaagg agcagtggct ggcgctcatg tccaaggact tgcgcctagc
     3481 cgcccaggca cgccctccac ttgagcccat gctggcgact ccgaccgcgg aggctttcga
     3541 caaccctgaa tgggggttcg agccgaagtg ggacgggatc cgagccctcg cagtctgcga
     3601 ggaggagacc cacctcatct cacgcaggaa ccacgacata actgtggcct atcccgagct
     3661 gcagcgcatc cacgaacaag tggttgctct cgacgccgtg ctcgacggcg agcttgtggc
     3721 cttcgacgac ggtgtgccct cctttcagac gctccagcag cggatgcatc tcagaaatct
     3781 gaagcacgtc gagcagttgt cactgagttt gccggtggtg tacatggtct tcgacctcct
     3841 ttatctggac ggccgagacc tgaccgacca gccactgcag gagcggcgtc ggatgctgga
     3901 agaagcggtg gttccatcag agcacatcca gatctcgccc tcggtcatcg gagatggggt
     3961 tgccctgttt cgggccgccg ctggacaagg cctggaaggg atcatggcca aacgcctgtc
     4021 gtccttgta
//
LOCUS       DBNHXU010000407         2108 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2751128, whole genome shotgun sequence.
ACCESSION   DBNHXU010000407 DBNHXU010000000
VERSION     DBNHXU010000407.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2751128"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..1992)
                     /locus_tag="AC161X_09925"
     CDS             complement(139..1992)
                     /locus_tag="AC161X_09925"
                     /inference="COORDINATES: protein motif:HMM:NF044955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2207 domain-containing protein"
                     /protein_id="HIR4989915.1"
                     /translation="MSRLSRLAIVVLLVVGPATAAFAKSFWLSNADVTVVVNDDGSLS
                     ITEILSFDFSGDFTGAYRDIPLEPGQRVTSVSVRDESTVYTPGGCTELGCSSPPGTYG
                     VAEFNDLVRIVWHHNSQDETRIFKLSYVVTGLATVYDDVVDVNYKVWGDQWSVGLDQL
                     HARVELPGQPVDGDVKVWGHPFDVDGVTSLGDDGVSPSLEARNIPAEQWVEIRVVFPT
                     DLLSATNGATVVSGDGLPTILAEEESFADDAEQAARAGRTGLIWGTVFALLTAVGLGG
                     FVYLRYGKEPRVDYDRDYEQEPPTDLSPAEVGALITQGSVTEKEFTATMFDLIRRGVI
                     AASPSQVERSTWGGLKNETITDLVLSVPEKEVELRNFEQSLMGVVRRVLELGPRPLHE
                     FRDAIRDDAAANAKSYDNFRDQSLGAVRRSGLLEDRGYGVSWAVRLIMAGAVIASFFL
                     LPRLLSRVPGGDVIAALISIGLLVGTILLFVLLSFRRVRNRRSKEGALESARWEAFRR
                     YLSDFSRLEEAPPISLELWDRYLVYAIVFGVAAEVLQQARLHAPPELETTSSIYWFGT
                     YGYSGGHSENAFAGIQSSLAGAFTPPSSSGGGGGFSGGGGGGGGGGGGGAW"
     gene            complement(1994..2092)
                     /locus_tag="AC161X_09930"
     CDS             complement(1994..2092)
                     /locus_tag="AC161X_09930"
                     /inference="COORDINATES: protein motif:HMM:NF015944.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989916.1"
                     /translation="MPTNLVAGAFGFTVREFFEAGPEAEQAPTVDF"
BASE COUNT          416 a          669 c          672 g          351 t
ORIGIN      
        1 gtggtgacgg gttgggtgca gcgtggggtc gtcagaaggc tgagcgctat gccgccgatg
       61 ctgggttcag tcgctttcag accctggagg acatcagcaa ccgattctcc gccttctatt
      121 tgctgtctgc atagcgaact accaggcgcc gccgcctcca ccaccgccgc caccacctcc
      181 accgccggag aaaccgccgc cacccccgct ggacgacgga ggggtgaagg ccccggcgag
      241 ggaggactgg atgccggcga atgcgttctc gctgtgaccg cccgagtagc cgtaagtgcc
      301 gaaccagtag atggacgacg tggtttccag ctcgggtggt gcgtgaaggc gagcctgctg
      361 aaggacttca gcggctacgc cgaaaacgat ggcatacacc aggtagcggt cccagagctc
      421 gagggagatc ggcggcgcct cctcgagtct cgagaagtcg gacaggtacc tgcgaaaggc
      481 ctcccatcga gccgactcca gagcgccctc cttggatcgc cggttcctga ctcgccgaaa
      541 actgaggagg acgaataaca ggatcgtccc caccagaagc ccaatggaga tcagagccgc
      601 gatcacatct ccgccgggca cgcgggacaa caacctggga agcaggaaga aggaggcgat
      661 cacggcacca gccatgatga ggcggaccgc ccatgaaacc ccgtagcccc gatcttcgag
      721 cagcccgctc ctccgcaccg cacccaggga ctgatcccgg aagttgtcat agctcttggc
      781 gttggctgcg gcatcgtccc gaattgcgtc ccggaactcg tggagtgggc gcggccccaa
      841 ttcgagaacc cgacggacaa cgcccatgag ggattgctcg aagttgcgaa gctcgacttc
      901 cttctccggg accgagagaa ccaggtcggt gatggtctca ttcttgagac cgccccatgt
      961 cgaccgttcg acctgggatg ggctggccgc gatgaccccc cgtcggatga ggtcgaacat
     1021 ggtcgcggtg aattccttct cggtgacact tccttgggtg atcagggctc cgacctcggc
     1081 cggcgagaga tcggtcggcg gctcctgctc atagtcgcgg tcatagtcca cccggggctc
     1141 cttgccatac cgcagataca cgaacccacc caggccgacc gccgtgagca gggcaaagac
     1201 agtcccccag atcaggccgg ttcgccccgc ccgggcagcc tgttcggcgt catcggcgaa
     1261 ggattcctcc tcggcgagga tggtcggcaa gccgtctccg gagacaacgg tcgccccgtt
     1321 ggttgcggac aggaggtcgg tggggaagac cacccgaatc tcgacccact gctcggccgg
     1381 tatgttcctc gcctccaacg aaggcgagac gccgtcgtca cccagtgagg tcactccgtc
     1441 cacgtcgaag ggatgacccc agaccttgac atcgccgtca accggctgtc cgggaagctc
     1501 gactcgggcg tggagttgat cgaggccgac tgaccactgg tcgccccaaa ccttgtagtt
     1561 gacgtcgacc acgtcgtcgt agacggtggc caacccggtg accacatagg aaagcttgaa
     1621 gatccgggtt tcgtcctgcg agttgtgatg ccagacgatt cgcaccagat cgttgaactc
     1681 ggcaactccg taggtgccgg gcggcgagct gcagccgagc tcggtgcatc cgccaggggt
     1741 gtagacggtc gactcatccc ggaccgagac cgaagtcacc cgctgacccg gctcgagggg
     1801 tatgtcgcgg tatgcccccg tgaagtcccc agaaaaatcg aagctgagga tttcggtgat
     1861 agacagcgat ccgtcgtcgt tgaccacgac ggtgacatcg gcgttggaga gccaaaagga
     1921 tttggcgaat gcggcggtcg ccgggccgac caccaataga acgacaattg ctagccggga
     1981 gaggcggctc aactcagaag tcgacggtcg gcgcttgctc cgcctcaggg ccggcctcga
     2041 agaactccct gactgtgaag ccgaaggcgc ccgcgacgag gttggtcggc acctgctgga
     2101 ccgtgttg
//
LOCUS       DBNHXU010000408         2305 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2555728, whole genome shotgun sequence.
ACCESSION   DBNHXU010000408 DBNHXU010000000
VERSION     DBNHXU010000408.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2305)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2305
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2555728"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..629)
                     /locus_tag="AC161X_09935"
     CDS             complement(<1..629)
                     /locus_tag="AC161X_09935"
                     /inference="COORDINATES: protein motif:HMM:NF019845.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HIR4989917.1"
                     /translation="MWGYRIHQWGSDPIWESIPDPHPDRDGVLIEVEACGIGRTVLNS
                     LAGDLTDERAALPRVPGHELVGLVVETGEDVDPSLVGTRVVAYFYLSCGSCSECLAGR
                     EQRCTVLKGWVGVHRDGGYAPLCALPARNIIPVSSDLDPVTATVIPDAVATPVHVAAR
                     AGIEASDLVVVIGAGGGVGSHMIQVARLHGAEVVGLDVGEQKLQSVADLG"
     gene            765..1490
                     /locus_tag="AC161X_09940"
     CDS             765..1490
                     /locus_tag="AC161X_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019349.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HIR4989918.1"
                     /translation="MAGEEQSTVSNGLTDAQPTTLLPEGLRLNADVRKRVTAVELVRD
                     VLRAGIIRGDLPGGSRLVQTDIASQLNVSTTPVREAMRDLASEGLITLDSHRIGTVRT
                     PDWDEMVEIVEVRRALERVAIEKAMANITPAQMQEATDLAEELAHEEDLGNWVLKNSA
                     FHSIFHRATGTKRLAGVLLALEEAGGVFVAQAQRLHPELRRQAVVDHFAMLDCYRSGD
                     LDGAVEIQYGHISLPLATPESIS"
     gene            1573..>2305
                     /locus_tag="AC161X_09945"
     CDS             1573..>2305
                     /locus_tag="AC161X_09945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008390659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxyltransferase"
                     /protein_id="HIR4989919.1"
                     /translation="MPGEPWKTDMWFSSPWNYLPEVTEGFQFPETLQVHDVTLRDGEQ
                     QAGVEFTADDKVRIAVALAEAGIHRIEAGLPAVSPADAEAVRRIVAEGLPTEVYAFSR
                     CMVPDVELALDCGAKGVVMEVPASHHLIEKAYNWPIERAIDASIEATKYAHDNGLEVT
                     FFPIDATRATMTDLLDDLEAVATHGHVDKIALVDTFGVTSPHAIQYYIRRVKERFDVP
                     LETHFHMDFGMGVANTVIAVAEGASV"
BASE COUNT          455 a          752 c          705 g          393 t
ORIGIN      
        1 ccgaggtccg cgaccgactg cagcttttgc tcgccgacgt ctagtccgac gacttcggct
       61 ccgtggagcc gcgccacctg gatcatgtgc gatcccacac cgccaccggc accgatgacg
      121 acaaccaggt ccgaggcttc gatcccggct cgagcggcga cgtgaaccgg ggtggccacc
      181 gcatccggta tcaccgttgc ggtcaccgga tcgaggtcgc tcgaaaccgg gatgatgttg
      241 cgagccggca aagcgcagag aggggcgtat ccgccatcgc ggtgcacgcc aacccagcct
      301 ttgaggactg tgcaccgctg ctcacgcccc gccaggcatt cggagcagga accgcaggac
      361 aggtaaaagt aggcgaccac cctggtgccg accagcgagg ggtcaacgtc ttcgccggtt
      421 tcgaccacta agccgacgag ctcgtggcca ggaacgcggg gaagggctgc ccgctcgtcg
      481 gtcaggtccc ctgcaaggct gttgagcacc gtgcgcccga tcccacacgc ctcgacctcg
      541 atgagaactc cgtcacgatc gggatgcgga tcggggatgc tttcccaaat cgggtccgag
      601 ccccactggt ggattcggta gccccacatt ttcaagccac ccctgtgtca ggggatttca
      661 gcgtcgggcc cccaattgtg tttgacactc tgcatactat atgacacgga atcggccctg
      721 attcgttata gttttcctgg tcacaaggcc ggatcggacg aggtatggca ggcgaagagc
      781 aatccacggt tagcaacgga ctgactgacg cccagccaac caccctcctc cccgagggtt
      841 tgcgccttaa cgccgatgtt cgaaagcggg tcaccgcggt cgagttggtg cgtgatgtgc
      901 tccgggccgg gatcattcgc ggcgatctgc ccggaggatc gcgcctggtt caaaccgaca
      961 tcgccagcca actgaacgta agcaccaccc cggtccggga ggcgatgcgc gacctggcga
     1021 gcgaagggct gatcacactc gacagccacc ggatcggcac cgttcgcacc ccagactggg
     1081 acgagatggt cgaaatcgtc gaggtgcgcc gcgccctgga gcgggtcgcc atcgagaagg
     1141 cgatggccaa catcaccccg gcacagatgc aggaggcgac cgatcttgcc gaggagctgg
     1201 cgcatgaaga ggacctggga aactgggtcc tcaagaacag cgcctttcat tcaattttcc
     1261 atcgggcgac cggcaccaag cgactggctg gcgtgctgct cgctctcgag gaggcgggcg
     1321 gagtgttcgt cgcccaagcg caacggctcc atcccgagct ccgccgccag gctgtagtcg
     1381 accacttcgc catgctcgac tgttatcggt cgggagacct cgacggggcg gtcgagatcc
     1441 agtacgggca catcagcctc cccctggcca ccccagagtc cataagttga ccctgcactg
     1501 tcctcttagg ggcccggaca cgctcgaaac tcgattgtat atgatgcagt ctgtcgaatc
     1561 tgaggagaat tgatgccagg agagccttgg aagaccgata tgtggttttc gagcccatgg
     1621 aactacctgc ccgaggtcac cgaaggtttc cagttccccg aaacactcca ggtccacgat
     1681 gtgacgctca gagacggtga gcagcaggcc ggcgtcgagt tcactgccga cgacaaggtc
     1741 aggatcgccg tcgccctggc cgaagccggg atccaccgca tcgaggccgg attgccagcg
     1801 gtgtcacccg ccgacgccga ggcggttcgg cgcatcgtgg ccgaagggct accaaccgag
     1861 gtctacgcct tcagccgctg catggtcccc gacgtcgagc ttgctctcga ctgtggggcc
     1921 aaaggcgtgg tcatggaagt cccggccagc caccatctca tcgagaaggc gtacaactgg
     1981 ccgatcgagc gggctattga cgcttcgatt gaagcgacca agtacgccca cgacaacggc
     2041 ctcgaagtca cctttttccc catcgacgcc acccgggcga cgatgaccga ccttctcgac
     2101 gacctcgagg cggtcgccac ccatggccac gtcgacaaga tcgcactggt ggacaccttc
     2161 ggggtgacct cgccgcacgc catccagtac tacatcagac gggtcaaaga gcgttttgac
     2221 gtcccgctcg aaactcactt ccacatggac ttcgggatgg gggtggccaa caccgtcatc
     2281 gccgtcgccg aaggggcctc ggtga
//
LOCUS       DBNHXU010000409         1738 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4739518, whole genome shotgun sequence.
ACCESSION   DBNHXU010000409 DBNHXU010000000
VERSION     DBNHXU010000409.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1738)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1738
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4739518"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="AC161X_09950"
     CDS             complement(<1..213)
                     /locus_tag="AC161X_09950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989920.1"
                     /translation="MDQSIARRAAIGVAVVIALIYIATVFVYTRIESWELADLGDTIG
                     FTVWIVAFTVVGVIIAVHRPGNPVAWI"
     gene            289..>1738
                     /locus_tag="AC161X_09955"
     CDS             289..>1738
                     /locus_tag="AC161X_09955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017623568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HIR4989921.1"
                     /translation="MTRSVPEKPALEGLEAKWMDRWRVDDTYRFDRSKTRGEIFSIDT
                     PPPTVSGSLHMGSVFGYVQTDAIARYQRMKGMEVFYPMGWDDNGLPTERRVQNYYGVR
                     CDPTLHYDPDFTPPTEPPDPPVSISRRNFIDLCHGLTAEDEKVFEDLWRTLGLSVDWA
                     QSYATIDETSQRTSQKAFLRNLKRGEAYSQEAPVLWDVDFKSAVAQAELEDRERPGAY
                     HRIAFHRSGGIPLYIETTRPELVCACVALVAHPDDQRYAELVGKTVTSPLFGVELPVV
                     AHELADPEKGSGIAMICTWGDTTDVTWWRELDLPTRSVIQQSGRLQEEVPEWIDSEGG
                     RAAYAEIAGLYPNQAQRKMAELLTEAGDLDGEPRPITHPVKFFEKGDRPLEIISSRQW
                     YIRNGGRDDELRQALLKRGDELDWVPDFMGVRFKHWVEGLNGDWLISRQRFFGVSVPV
                     WYPIDSAGELDFEHPILPDLADLPVDPTTDVPP"
BASE COUNT          334 a          544 c          549 g          311 t
ORIGIN      
        1 gatccatgcc accgggttgc ccggccgatg aacggcgatg atgacgccga ccacggtgaa
       61 cgcaacgatc cagacggtga atccgatagt gtctcccagg tcggccaact cccatgactc
      121 gatcctggtg tagacgaaca ccgtggcgat gtagatcaac gcgatgacaa cggcgacgcc
      181 gatcgccgcg cgccgggcga tggactggtc cacagccaaa acctaggccg cggtcgttcc
      241 gagagcgtta cggcctggtc ccttgcgacc gttaccctta accgccaaat gacccgatcc
      301 gtccccgaaa aacctgctct cgaaggactc gaggcgaagt ggatggaccg gtggagggtc
      361 gacgacacct atcgattcga ccgctccaag acccgcggcg agattttctc catcgacacc
      421 ccgcccccga ccgtctccgg ctcgttgcat atggggtcgg tgttcggata cgtccaaacc
      481 gacgccatcg cccgttacca gcggatgaag gggatggagg tcttctatcc gatgggttgg
      541 gacgacaacg gcctccccac cgagcgtcga gttcagaact attacggggt ccgatgtgac
      601 ccgaccctcc actacgaccc cgacttcacc ccgcccaccg agccgcctga cccgccggta
      661 tcgattagcc ggcgcaactt catcgatctc tgtcacgggc taaccgccga ggacgaaaag
      721 gtcttcgagg atctgtggcg cactctcggc ctatcggtgg attgggctca gagctacgcc
      781 accatcgacg agacctcgca acgaaccagc cagaaggctt ttctccgcaa tctcaagcgt
      841 ggggaggcct attcccaaga ggcgccggtg ctttgggacg tcgatttcaa atcggctgta
      901 gctcaggctg aattggagga ccgggaacgg cccggggcct atcaccggat cgcgtttcat
      961 cggtcgggtg ggatcccgct ctacatcgag accacccgac ccgagctggt ttgtgcctgc
     1021 gtggccctgg tggctcatcc cgacgaccag cgatatgccg aactggttgg caagacggtg
     1081 acctcgccgc tgtttggggt cgagttgccg gtggtggccc atgagctggc cgatcctgaa
     1141 aaggggtccg ggatcgccat gatctgcacc tggggcgaca ccaccgatgt gacctggtgg
     1201 cgcgagctcg acctgccgac caggtcggta atccagcaga gtgggcggct ccaggaggag
     1261 gtgccggagt ggatcgattc cgagggggga cgcgctgcct atgcggagat cgccgggctc
     1321 taccccaacc aggcccagcg caagatggcg gagttactga cggaagcggg ggatctcgac
     1381 ggcgagccgc ggccgatcac ccacccggtg aagttcttcg agaagggcga ccgacctctg
     1441 gagatcatct ccagccggca gtggtacatc cgaaacggcg ggcgggatga cgaactgcgc
     1501 caggcgctcc tgaaaagggg tgatgagctg gattgggttc cggatttcat gggggtgcgc
     1561 ttcaaacatt gggtggaagg tctcaacggt gactggctga tctcgcggca aaggttcttt
     1621 ggggtctccg tcccggtctg gtacccgatc gacagcgccg gtgagctcga cttcgagcac
     1681 ccgatccttc ccgacctggc tgacctgcca gtggacccta cgaccgatgt ccctccgg
//
LOCUS       DBNHXU010000410         2028 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1762891, whole genome shotgun sequence.
ACCESSION   DBNHXU010000410 DBNHXU010000000
VERSION     DBNHXU010000410.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1762891"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..271)
                     /locus_tag="AC161X_09960"
     CDS             complement(<1..271)
                     /locus_tag="AC161X_09960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989922.1"
                     /translation="MDEKKLLATGVTLLLSLIVYLVVAKIGARFVEKIGARGPEAASR
                     ASTLWLMVRRVLQFTLILFVVLMVFSIWGWSVAPFIAIGTVIAAAV"
     gene            complement(287..1486)
                     /locus_tag="AC161X_09965"
     CDS             complement(287..1486)
                     /locus_tag="AC161X_09965"
                     /inference="COORDINATES: protein motif:HMM:NF014036.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HIR4989923.1"
                     /translation="MRDGITGLIPVIDQAVADCVGVVDPASLETVALMRKRIVTRLDY
                     PDDVLFAALAGGTGSGKSSLFNAIAGEDIAPTGGVRPTTSQPKALVPSSRAEAVAGYL
                     DLIGVADRVPHSQHQWLCLIDLPDTDSVEVDHRQRVEALLPQIDFVIWVVDPEKYRDA
                     SIHREYLAPMAAYQEQFLFVLNQRDRLSDDDLSRVVADLSAALTEDGIRRPTVIATAA
                     GPSAGPAQGIDQLVAHLQRELGGSVYEKALVDLRTAADSLLQMLGGGSSSEFDVRWSE
                     IVDEVTAQANAGDLSDASQQLVAFLDDLSDEVGGEIGNAIGALATEAPRVILSAEPDV
                     APNSPPPRWWRRSSREDPPQQDTLREALEKRIARPTRDLLTRRAKAQASVAELALALN
                     NRTPRPR"
     gene            complement(1479..>2028)
                     /locus_tag="AC161X_09970"
     CDS             complement(1479..>2028)
                     /locus_tag="AC161X_09970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989924.1"
                     /translation="ANARLRRVRSWMRRKAPGAESIEAAKRRVSETLATAVAIDLKAS
                     ITDGGVAVHDQSPSSYRLGTPTQGRIARAIDSWWMLLDEMSRSAIGRPDVTAHLLGVA
                     ALQEDPGLELVIAAVFPGTDPLAVIEGAREALVWALEPVYSSVRNPIAFAVAREATPA
                     QEVERARTVVAEVVARSAFADA"
BASE COUNT          379 a          671 c          645 g          333 t
ORIGIN      
        1 cgacggcggc ggcgatcacg gtgccgatgg caatgaacgg ggccacgctc cagccccaga
       61 tcgagaagac catcagcacc acgaacagga tcagcgtgaa ctgcaagacc ctgcgaacca
      121 tcagccagag cgtgcttgcc cgcgatgccg cttccgggcc gcgggcaccg atcttctcaa
      181 cgaaccgggc gccaatcttg gccacgacga gatagacgat cagagagagg agaagggtga
      241 ctccagtcgc gaggagcttt ttctcgtcca tggccggcga gggtacctac cgaggtcgcg
      301 gggtccggtt gttgagggcg agcgcaagct cggccaccga ggcctgggcc ttggcgcgcc
      361 gggtcaggag gtcacgtgtc ggccgggcta tccgtttctc cagagcctcc cgcaacgtgt
      421 cctgttgtgg tgggtcctcg cgagatgacc gtcgccacca tctcggcggg ggagagttgg
      481 gagccacgtc aggctcagcc gagaggatca cccgaggtgc ctccgtcgcc agcgcgccga
      541 tcgcattccc gatctcgcct cccacctcat cggacagatc gtcgagaaag gcgacgagct
      601 gctgtgaggc atccgacaga tctccggcat tggcttgagc ggtcacttcg tcgacgattt
      661 cgctccaccg aacgtcgaat tctgagctcg atccccctcc gagcatctgg agcaacgagt
      721 cggccgccgt gcggaggtcg accaaagcct tctcgtagac cgagcctccc agctcacgct
      781 gcagatgagc caccagctga tcgatcccct gagccggccc agccgatggt ccggcagcgg
      841 tcgcgatcac agttggccgc cgaatgccgt cctcggtcag ggccgccgac aggtcggcca
      901 ccactctcga caggtcgtcg tcgctcagcc gatcccgctg attgaggaca aacagaaact
      961 gctcctgata ggcggccatt ggcgcgaggt actcgcgatg gatcgaggca tcccggtact
     1021 tctcaggatc gaccacccag atcacaaagt cgatttgtgg gagtagagcc tcaacacgtt
     1081 gccgatgatc gacttcgacg ctgtcggtgt ctggcaggtc gatgaggcac aaccattggt
     1141 gctgggagtg tgggacccga tcagccactc cgatcaggtc gagatagccg gcgaccgcct
     1201 ccgcgcggga cgacgggacc agagctttgg gctgcgaagt cgtaggtcgg actccgccgg
     1261 tgggggcgat gtcctcgcct gcaatcgcat tgaacaggct ggacttgccg gagccggtgc
     1321 cgccggccaa agccgcaaac aacacgtcgt cggggtaatc cagccgcgtc acgattctct
     1381 tccgcatcag ggcgaccgtt tccagggagg cggggtctac gacgccgaca caatcggcta
     1441 cggcctggtc gatcaccggg atcaggccgg tgatcccgtc acgcatcggc gaacgccgac
     1501 cgcgccacga cttcggcgac gacggtccgt gcccgttcga cctcctgggc gggggtggcc
     1561 tccctggcca cagcgaaggc aatgggattg cgaaccgatg agtagaccgg ctcgagggcc
     1621 cagaccagcg cctcccttgc gccttcgatc acggccaagg gatcggtccc aggaaaaacg
     1681 gcggcgatga ccagctcgag cccaggatcc tcttgcagag ccgccacccc caacagatgg
     1741 gccgtcacat cgggtcgacc aatagccgac cgggacatct cgtccaggag catccaccac
     1801 gagtcgatcg ccctggcaat cctcccctga gtgggcgtcc cgagccgata cgacgaggga
     1861 ctctggtcgt ggaccgcaac tcctccatcc gtgatcgagg ccttgaggtc gatggcaaca
     1921 gcagtggcga gtgtttccga gactcgacgc tttgctgcct cgatcgactc cgcgcccggc
     1981 gccttccttc gcatccacga gcgcactcgc cggagacggg cgttggcg
//
LOCUS       DBNHXU010000411         3681 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1367945, whole genome shotgun sequence.
ACCESSION   DBNHXU010000411 DBNHXU010000000
VERSION     DBNHXU010000411.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3681
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1367945"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..700)
                     /locus_tag="AC161X_09975"
     CDS             complement(<1..700)
                     /locus_tag="AC161X_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012974.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DnaB-like helicase N-terminal domain-containing
                     protein"
                     /protein_id="HIR4989925.1"
                     /translation="MSSLTEQTEMRPGYRSPPHSREAEESVIGAVLLSVDAVNEVMDR
                     LHPEDFYIPAHQAIFESMRQLFDGNQAIDAVTVSEELRRRGELEKVGGVNYLTRLVDI
                     VPSTSNIVYYAGIVEEHGKRRELIRAGATVTEVAFNIDEEIAIVLDRAEQTVLGVAER
                     RSSQTLLEVGPLFSDVLEHIEALEQQGSEINGLATGFVDLDSKLAGIQPANLVVIAGR
                     PGMGKSAIALNIATN"
     gene            complement(968..1417)
                     /gene="rplI"
                     /locus_tag="AC161X_09980"
     CDS             complement(968..1417)
                     /gene="rplI"
                     /locus_tag="AC161X_09980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HIR4989926.1"
                     /translation="MKLILISDVNELGKRGDVVDVAEGYARNFLLPRKKAVKASEGSM
                     AQAEATREARIENEQRAREAAENVATQLVGTRVVLAAQAGDEGRLYGSISVNDVVEGI
                     KRFTGLELERDSVVIATPIKAIGLHEIEIKLHPEVEFPLLLDVIPAT"
     gene            complement(1414..1638)
                     /gene="rpsR"
                     /locus_tag="AC161X_09985"
     CDS             complement(1414..1638)
                     /gene="rpsR"
                     /locus_tag="AC161X_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011563835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HIR4989927.1"
                     /translation="MEVRRGRPKPCAFCKARVEYIDWKDVSVLRKYISDRGKIRGRRV
                     NGNCPRHQREIAVAIKSAREMALLPYLSTR"
     gene            complement(1707..2156)
                     /gene="ssb"
                     /locus_tag="AC161X_09990"
     CDS             complement(1707..2156)
                     /gene="ssb"
                     /locus_tag="AC161X_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020019276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HIR4989928.1"
                     /translation="MSNNVTLIGNLVDDPELRFTPSGVAMAKIRMAVNRRWRGNDGEW
                     QEDTSFFTGTVWREQAESAAESLQKGARVIVTGRLEQRTWETDQGDKRSVVEVQIDEI
                     GPSLRWATATVNKTARSGAGDFPSPQGGDVPAAPVARDSYGPDEAPF"
     gene            complement(2217..2633)
                     /gene="rpsF"
                     /locus_tag="AC161X_09995"
     CDS             complement(2217..2633)
                     /gene="rpsF"
                     /locus_tag="AC161X_09995"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HIR4989929.1"
                     /translation="MGKATESGYTSSDRTDVSRRWDACNPCSIKWMGPSIRMSTGGRM
                     RDYELMTIHRPDLAEDDIDAKIGELESYLSEHGASVKGKDLWGKRRFAYEIEHLSEGY
                     YSVVNFQAEAGAVDSLDRMLALADEVVRHKIVRLGG"
     gene            complement(2617..3027)
                     /locus_tag="AC161X_10000"
     CDS             complement(2617..3027)
                     /locus_tag="AC161X_10000"
                     /inference="COORDINATES: protein motif:HMM:NF014678.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HIR4989930.1"
                     /translation="MLLTACSTSQQVVETTSVQVGGVPLEVLIADTPEERQLGLTGVE
                     VIPGGADGMLFVYETPAPVRYFMLDVPIALDIWFFDPDGVLIGGTEMAPCPAEPCPLY
                     PSPADVSWVLETVAGVYEFDEGAALTGAPNGESD"
     gene            complement(3042..>3681)
                     /gene="panB"
                     /locus_tag="AC161X_10005"
     CDS             complement(3042..>3681)
                     /gene="panB"
                     /locus_tag="AC161X_10005"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007920307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HIR4989931.1"
                     /translation="AVKRAQVSALFVVDMPWLSFHISPEDAVRNAGRIVQEGGAEAVK
                     LEGGRKRIPAIERILDAEIPVMGHLGLTPQSVNAMGGFKVQGKVVDAAREMIEDAHAL
                     ADAGCFSIVLEGVPDVLGELITKEVDVPTIGIGAGPNTDGQVLVYHDVLGIGLGHHAK
                     FVRSYANVGEESIEALKSFAADIQSGAFPGEEESYHVSDEVAAELLRGKSSD"
BASE COUNT          661 a         1197 c         1133 g          690 t
ORIGIN      
        1 tgttggtggc gatgttcaag gcgatggccg acttgcccat gcccggtcgc ccggcgatca
       61 caaccaggtt ggccggctgg attcccgcga gcttcgagtc gaggtccacg aacccggtgg
      121 ccaggccgtt gatctcggat ccctgctgct ccagggcctc gatgtgctcc aggacatcac
      181 tgaacaaggg gccgacttcg agcaaggtct gcgaactgcg ccgttcggcg acgccaagca
      241 cggtctgctc ggcgcggtcg aggacgatgg cgatctcttc gtcgatgttg aaggcaacct
      301 cggtgaccgt ggctccggcc cgaatcaact cccgtcgttt gccgtgctcc tcgacgatcc
      361 cggcgtagta gacgatgttc gaggtggatg ggacgatgtc gacgagacgt gtcaggtagt
      421 tgaccccgcc cactttctcg agctctcccc ggcgacgaag ctcttcggag acagtcactg
      481 cgtcaatcgc ctggttgccg tcgaacagct ggcgcatcga ctcgaagatc gcttgatggg
      541 ccgggatgta gaaatcctcg gggtggagcc ggtccatcac ctcgttgacg gcatccaccg
      601 agaggagcac ggctccgatc accgactcct ccgcctcacg tgagtggggc gggcttcggt
      661 agccgggacg catctcggtc tgctcggtca ggctgctcat ctactctctt ctaccaacgc
      721 cgtgtgacag ttttcgcgaa ccgacgcgtc aaaccctgac ggcttggggg aagatcggcc
      781 agcttcgggg gaagcgtccg gaacatagac caccggcggc cggcattcca aaactccgcg
      841 aaagactgcc aaaagacgcg aaatcggagc ctggaacccg cgaaaactga cgctagttgc
      901 gcacggtctc cacgaaattg ggttcctcag gggtcatata cggacctgtg gatcccaatt
      961 agggggccta ggtggcgggg atgacgtcga ggaggagtgg gaactcgacc tctgggtgga
     1021 gcttgatctc gatctcgtgg agcccgattg ccttgatcgg agtggcgatc acgaccgagt
     1081 cacgctccaa ctcgagaccg gtgaaccgct tgattccctc gaccacgtcg ttgaccgaga
     1141 tcgacccgta gagccttccc tcgtcaccgg cctgagccgc gagaacgacc cgggtgccca
     1201 cgagttgggt ggcgacgttc tccgccgcct cccgagctcg ctgttcattc tcgatgcggg
     1261 cctcgcgggt ggcttcggcc tgagccatgg agccctcgct cgccttcacc gccttcttcc
     1321 tcggcaggag gaagtttcgg gcataaccct cggcgacgtc gacgacatcg ccccgtttcc
     1381 cgagctcgtt gacatcggag atgaggatca gcttcatcgg gtgctcagat agggcagcaa
     1441 ggccatctca cgggcgctct tgatggcgac cgcgatctcg cgctggtggc gaggacagtt
     1501 cccattgacc cgacgacccc ggatcttgcc gcgatcggag atgtacttgc gcaggacgga
     1561 gacgtccttc cagtcgatgt actcgaccct tgccttgcag aacgcacagg gcttcggacg
     1621 accccggcgc acttccatgc cgcgacgtcg ctttggccgc gggttgttcg tggatttgcg
     1681 tgccatctag tttgaaactc ctgaaatcag aacggggcct cgtcgggccc atagctgtca
     1741 cgggcgaccg gggccgcagg aacgtcccca ccctgtgggc tgggaaagtc accggcgccg
     1801 gagcgcgccg tcttgttcac cgtcgccgtt gcccagcgca gggaagggcc gatctcgtcg
     1861 atttggacct cgactacaga tcgcttgtcc ccctggtcgg tctcccaggt ccgctgctcg
     1921 agccgcccgg tgacgatcac ccgtgcgccc ttctgcaacg actcggcggc ggattccgcc
     1981 tgctctctcc acacggtgcc ggtgaagaag ctggtgtcct cctgccattc gccatcgttg
     2041 ccccgccagc ggcgattgac cgccatgcgg atcttggcca tggccacccc agatggggtg
     2101 aagcgcagct cggggtcatc gacgaggttg ccgatgagtg tgacgttgtt cgacattgag
     2161 atctcctctt ctcacgccca ccgacgatca tgccgcgccg gtgtgacgta ttccagtcag
     2221 ccgcccagcc gcacgatctt gtgtcgaaca acttcgtcgg cgagggccag catccgatca
     2281 agtgagtcga cggctcccgc ctcagcctgg aagttcacca ccgagtagta gccctcggag
     2341 agatgctcga tctcgtaggc gaagcggcgc ttcccccaga gatccttgcc cttgacactc
     2401 gctccgtgct cggaaaggta cgactcgagc tcgccgatct tggcgtcgat gtcgtcctct
     2461 gcgagatcag gacgatgaat cgtcattagt tcatagtcgc gcattcgacc tcctgtggac
     2521 atccgtatgg aaggccccat ccatttgatg gagcaggggt tgcacgcatc ccaacggcgc
     2581 gagacgtcgg tgcggtcgct cgaagtgtat ccggattcag tcgctttccc cattcggggc
     2641 gccggttaac gctgcgcctt cgtcgaactc atagacgccg gccacagtct ccaagaccca
     2701 ggacacatcg gcaggggacg gatagagcgg gcagggctcg gccgggcacg gagccatctc
     2761 ggtgcctccg atgaggaccc catcgggatc gaagaaccag atgtcgagtg cgatcggcac
     2821 atccagcatg aagtaccgaa ccggagccgg agtctcgtag acgaagagca tcccgtcggc
     2881 cccgcccggg atcacctcga ctcccgtcag accgagctga cgctcctcag gagtgtctgc
     2941 gatgaggacc tccaggggaa ccccgccaac ctggaccgac gtggtctcaa cgacttgctg
     3001 ggaggtggag cacgccgtca gaagcaagac acaggcgaaa gtcagtcgga cgacttgccc
     3061 ctgagcaact cggcggcgac ttcgtccgac acgtgatagc tctcctcctc gccggggaag
     3121 gcgccggact gaatgtcggc ggcgaagctc ttcagggctt cgatcgactc ctcacccaca
     3181 ttggcgtagc tccggacgaa cttggcatgg tggccgaggc ctatcccgag gacgtcgtgg
     3241 tacacgagga cctggccgtc ggtattgggg ccggcgccga tcccgatcgt gggcacgtcg
     3301 acctccttgg tgatcagctc accgaggaca tcggggacgc cttcgaggac gatcgagaag
     3361 caacccgcgt cggcaagagc gtgagcatcc tcaatcattt ccctggcggc gtctacgacc
     3421 ttcccctgca ccttgaaacc acccatcgca ttgaccgact gcggagtgag cccaaggtgc
     3481 cccatcaccg ggatctcggc atccaggatg cgctcgatcg ccgggatccg cttgcggccg
     3541 ccctccagct tcacagcctc cgcgccgccc tcctgaacga tccggcccgc attgcgcacc
     3601 gcgtcctctg gcgagatgtg aaagctcagc cagggcatgt cgaccacgaa gagggccgaa
     3661 acctgggcgc gcttcaccgc c
//
LOCUS       DBNHXU010000412         2872 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5139319, whole genome shotgun sequence.
ACCESSION   DBNHXU010000412 DBNHXU010000000
VERSION     DBNHXU010000412.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5139319"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..389
                     /locus_tag="AC161X_10010"
     CDS             <1..389
                     /locus_tag="AC161X_10010"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HIR4989932.1"
                     /translation="SLFGRHQGVEVSHTGDGFFVTFDRPSDAVEAAVAIQRRMTEHRR
                     ESGFAPWVRLGIHQAEVTKDGGDYRGQGVHVAARVGAVAEEEEIVISAAALSVAGEIK
                     FPLSKSRSVELKGVLEPVEVSTVDWR"
     gene            450..833
                     /locus_tag="AC161X_10015"
     CDS             450..833
                     /locus_tag="AC161X_10015"
                     /inference="COORDINATES: protein motif:HMM:NF024092.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HIR4989933.1"
                     /translation="MSEHPNAAMYRDVMEKFNAGDTNAMVDSLADDVVWWQIGSAEPL
                     MGKAALMESMAGLGEMPITVDLTDVVANDDMVVGLVTATVGSGPEAFSYRTAEVAWLK
                     DGKVAERRAYSDDTEAINNFFNQFS"
     gene            complement(835..1323)
                     /locus_tag="AC161X_10020"
     CDS             complement(835..1323)
                     /locus_tag="AC161X_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024100.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HIR4989934.1"
                     /translation="MVDSQELEASLEAGERMLDQEQYQEAIDHFIDLANQNPGNPRVT
                     LHVAGAYDSAGREEEAVAPYRAALAAGLDEEDDLRARIQLASTLRNLGQNQEAVEILT
                     RVCEENPRHRAARAFLALALTSADRGPDAVSELLDLLLTNPGPLEAYQRSLRWYADDL
                     RS"
     gene            complement(1372..1674)
                     /locus_tag="AC161X_10025"
     CDS             complement(1372..1674)
                     /locus_tag="AC161X_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989935.1"
                     /translation="MSTSVVGSPRAPQSIGRSRAVGAGLAALVLALGIGLGLSADRSD
                     PTRVVDANVTRLEPSIQARRLQMTAEQRALRLQAIIEHRTELALRHTPFDGSIGRS"
     gene            1765..>2872
                     /locus_tag="AC161X_10030"
     CDS             1765..>2872
                     /locus_tag="AC161X_10030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989936.1"
                     /translation="MRRTTGQVAAAAVTGGVLGAVGGAALGSSSGNAASGAVLGGIIL
                     AVISGWADATRKPGEPQALAARIAGSVFIAATIGWLLAWLLPDWPVWVVAVMAGVGTG
                     LTGLRPLKVLLGLVVGAVIGFTLYLVSPDIGWPVASALTVVTYRTLAAFLWRGKEQLR
                     VMGERAPEADLPFVVPFSEATKHVGVDYLERYARSSGAHFAHSPADVGIVSDFDELAG
                     PTFDPTIVHPLIHEFYEHTSRFKLSIVPEWKPWMRLPYRIYRATVAKPLGQANAPFEI
                     EEVQRGVISWIDTIDVDNDGTADFRAWIRAYEDGEPIYVGIYTVIHVEDMAYVAVGFP
                     LPSGNFSATLQPFNNRGDGLLLRSHTDRALPGHYL"
BASE COUNT          499 a          855 c          950 g          568 t
ORIGIN      
        1 gctcgctgtt tggcaggcac cagggtgtcg aggtcagcca cacaggcgac ggcttcttcg
       61 tgaccttcga ccgtccttcg gacgcggtcg aagcggcggt cgccattcag cgccggatga
      121 ccgagcaccg gcgagagtca ggttttgccc cgtgggtgag acttgggatc caccaagccg
      181 aagtcaccaa ggacggcggc gactatcggg gtcagggagt gcacgttgcg gctcgggtgg
      241 gtgcggtggc cgaggaggag gagattgtga tctctgctgc agcgttgtcc gtagccgggg
      301 agatcaagtt cccgctctcc aagtcccgct ccgttgagct caaaggagtg ctcgagccgg
      361 tcgaggtatc gactgtcgac tggcggtaac ccaccgagtc tctcggagga gtaggttcaa
      421 ggaacgctct ccgtcccaaa ggaggaacca tgagcgagca tccaaacgcc gcgatgtacc
      481 gcgatgtcat ggagaagttc aatgccgggg acacaaatgc aatggtcgac tcgcttgccg
      541 acgatgtcgt ctggtggcag atcggctcgg ctgagccgtt gatgggaaag gctgccttga
      601 tggagtcgat ggcagggctc ggcgagatgc caatcaccgt cgacctgaca gacgtggtcg
      661 ccaacgacga catggtggtc ggcctggtca cggcaaccgt gggctcaggc cccgaggcct
      721 ttagctatcg gacggctgag gttgcctggc tcaaggacgg caaggtcgcc gagaggcgtg
      781 cctactcaga cgacacggaa gccatcaaca acttcttcaa ccagttcagc tgactcagga
      841 gcggagatcg tccgcatacc agcgcaatga acgttggtag gcctcgagcg gaccggggtt
      901 agtgaggaga agatccagca actctgacac cgcgtcgggc cctctgtcgg cgctggtcaa
      961 tgccagggcg aggaaggctc gggcggcccg atgtcgagga ttctcctcac aaacccgggt
     1021 cagaatctcg accgcctcct ggttctggcc aagattgcga agggtgctgg ccaactgaat
     1081 tcgagctcgc aggtcgtctt cctcgtcgag gccggccgca agggcggcgc ggtaaggggc
     1141 gacggcctct tcttcccttc cggccgagtc gtaggcgcct gccacgtgaa gtgtcaccct
     1201 cggattcccc ggattctgat tagccaggtc aatgaagtga tcgattgctt cctggtattg
     1261 ctcctgatcg agcatccgct cgccggcttc tagcgaagcc tccagttctt gcgaatcgac
     1321 catgaatcga ctgtaccgct ggcgttgcgg gccgttgatc tagggagcct ttcagctgcg
     1381 accgatcgaa ccgtcgaacg gggtatgtct cagggccagt tcggtgcgat gctcaatgat
     1441 ggcctggagg cgcagggcgc gctgttccgc agtcatctga agacgtcggg cctgaatcga
     1501 tggctcgagt ctggtcacgt tggcgtcgac gacccgtgtt gggtccgagc gatcggcgct
     1561 gaggccgagt ccgatgccga gggccaggac gagagcggcg agtccggccc cgactgctcg
     1621 agatcggcca atggattggg gtgccctggg tgaaccaacg acactcgtgg acatgacttc
     1681 ctcctcacga tcacgtcgtg gcatcgatgc tcacgacatc agcgaagagg tcatcgctcg
     1741 cccgttgata cgtagcttcc gatcgtgaga cgcaccacgg gccaggtcgc cgctgccgca
     1801 gtcactggtg gcgtgttggg tgcggtgggt ggtgccgccc tcgggtcgtc gagcggaaat
     1861 gcggcttcgg gggctgtcct cgggggcatc atccttgcgg tcatctcagg atgggctgac
     1921 gccaccagga agccggggga gccccaagct ctcgcggctc gcatcgccgg atccgtcttc
     1981 atcgccgcca ccatcggatg gctgctggcg tggcttttgc ctgactggcc ggtgtgggtg
     2041 gttgccgtta tggccggcgt aggcacgggc cttaccgggc tcaggccgtt gaaggtgctg
     2101 ctcggactgg tggtgggggc cgtgatcggt ttcacccttt acctggtgag tcctgacatt
     2161 ggctggccgg ttgccagcgc tttgaccgtg gtcacctatc gcacgctcgc cgcctttctc
     2221 tggcggggaa aggaacagct ccgggtcatg ggggagcgag ccccggaggc tgaccttccg
     2281 tttgtggttc ctttctccga ggcgacgaag catgtgggcg tcgactacct cgagcgctat
     2341 gcccgctcat cgggcgcgca tttcgcgcac agccctgccg atgtggggat cgtcagcgac
     2401 ttcgacgagc tagcagggcc aaccttcgac ccgaccatcg tccacccatt gatccatgag
     2461 ttctacgagc acaccagtcg cttcaagttg tcgatcgtcc ccgagtggaa accctggatg
     2521 cgtctgccct atcggatcta tcgggcgacc gtggccaagc ccctcggtca ggcaaacgcc
     2581 ccgttcgaga tcgaggaggt gcagcggggg gtcatctcct ggatcgacac catcgatgtc
     2641 gacaacgacg ggaccgccga ctttcgggcc tggattcggg cttacgaaga cggcgagccg
     2701 atctacgtcg ggatctacac ggtgatccat gtcgaggaca tggcatacgt ggctgtcggg
     2761 ttcccactcc cgtccggcaa tttcagcgca actcttcagc cgttcaacaa ccgaggtgat
     2821 ggcttgttgc tccggtcgca caccgatcgg gccttgcccg gccactacct gt
//
LOCUS       DBNHXU010000413         1883 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1369026, whole genome shotgun sequence.
ACCESSION   DBNHXU010000413 DBNHXU010000000
VERSION     DBNHXU010000413.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1883)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1883
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1369026"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="AC161X_10035"
     CDS             complement(<1..308)
                     /locus_tag="AC161X_10035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989937.1"
                     /translation="MPELTQSPAVDEKAPAGRRPITRWTGPGWLWLLLLIIIVVASLV
                     AENFLSSRNLSNILRQMIPLGIVAVGQTLVILIAGVDLAVAATIGMANTALMGIVAGQ
                     A"
     gene            complement(301..1821)
                     /locus_tag="AC161X_10040"
     CDS             complement(301..1821)
                     /locus_tag="AC161X_10040"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HIR4989938.1"
                     /translation="MLKVEGISKQFDGVHALTSVDAEFKAGEVHAVVGENGAGKSTLV
                     KIMAGVHGADSGTLFLDGDEVTFASPRDAIDASISVVYQEPSLVPMLTVEDNLILGRE
                     PTRRGFISNGKAREFSIDALRMVGASIDPGSPVERLTIAERQLVEIAKALSLHSRMIL
                     LDEPTSSLSITEIERLRGVVEDLRSRDVAVVLVTHDLDEVFLLADRVTVLKDGKVSLT
                     SMVADVTAAEVIKAMIGRDLAHMFPPRTARERASKPVVQVKGLTIPGRAEDISFELRE
                     SEVVGFVGLIGAGRTDVAKALVGATPSRRGDILIDGVDANVREPADAVALGLALVPED
                     RLADGLVMDLGVKQNIGLPQLNALSTWGVIDKSAEAALADGQIESLSIRTPSNSTVVH
                     NLSGGNQQKVVLGRWLAKGCRVLILDEPTRGVDVGAKAEIYRIIRQLADEGTAVMLIS
                     SELPEILHLSDRIIVMSKGRIVAEIENTSTSERDLASEEDLVKLALGLQAEGSTRA"
BASE COUNT          359 a          613 c          555 g          356 t
ORIGIN      
        1 gcctgtccgg cgactattcc catcagcgcg gtgttggcca tgccgatcgt cgctgcgaca
       61 gcgaggtcga cgcctgcgat gaggatgacc agtgtctgac caacggccac gatcccgagc
      121 gggatcattt ggcggaggat gttgctcagg ttgcgcgaac tgaggaagtt ctcggccacc
      181 agagaggcca ccacgatgat gatcaacagg agcagccaaa gccagcctgg gcccgtccag
      241 cgggtgatgg gacgcctgcc tgcgggcgcc ttctcgtcga ccgcaggtga ctgagtcaac
      301 tcaggcacgg gtcgagcctt ccgcctgaag tccgagcgcc agcttcacta aatcctcttc
      361 actagccaga tctctttcac tggttgaagt gttctcaatc tcagcaacga ttcgaccctt
      421 ggacatgaca attatccggt cgctgagatg cagaatctcg ggtagttccg acgagatcaa
      481 catcacggcg gtgccctcgt cggcgagctg gcgaataatc cggtagatct ccgccttggc
      541 ccccacgtct accccccgcg tgggctcatc gaggatcagg acccgacacc ccttcgccag
      601 ccagcgtccc aacacaacct tctgttggtt cccaccgctg aggttgtgga ccacggtcga
      661 gttcgacggg gttcggatgc tcagcgactc gatctggccg tcggcaagcg ccgcctcggc
      721 gctcttgtcg atgacccccc aagtgctcag tgcattcagt tgcgggaggc cgatgttctg
      781 cttgacaccc aggtccatga ccagaccatc cgcaagtcga tcctcgggaa ccagagcgag
      841 tccgagagcc acggcgtcgg ccggctcgcg gacgttggca tcgacaccat cgatgaggat
      901 gtcgccacgc cggctcggtg ttgccccaac caatgccttg gcgacgtcgg tccgcccggc
      961 tccgatgagg ccaacgaacc cgacgacctc ggattctctc aactcgaaac tgatgtcctc
     1021 ggccctcccc gggatggtga gccctttgac ctggaccacc ggtttcgaag cgcgttcccg
     1081 agcagtcctt ggggggaaca tgtgggccag atctcgcccg atcatcgcct tgatcacctc
     1141 cgcggccgtc acgtccgcga ccatcgaagt cagtgacacc ttgccgtcct tgaggacagt
     1201 gacccggtcg gcgaggagga acacctcatc gagatcgtgc gtcaccagga ccaccgccac
     1261 atcgcggctc cgcaggtcct cgacgacgcc ccgaagtctt tcgatctcgg tgatcgagag
     1321 cgatgatgtc ggctcgtcga ggaggatcat ccgggaatgg aggctcagcg ccttggcgat
     1381 ctcgaccagt tgccgttcgg caatggtcag ccgctcaacc ggggaccctg gatcgatgga
     1441 cgccccaacc atccgcagcg cgtcgataga gaactccctg gctttcccat tggaaatgaa
     1501 tcctcgccgg gtcggctcgc ggcccaagat caggttgtcc tcgactgtca gcatcggaac
     1561 cagagatggc tcctggtaga cgaccgaaat gctggcgtcg atcgcgtcac gaggtgaggc
     1621 gaaggtgact tcatccccgt cgagaaacag tgtgccgctg tcggctccat ggacgccggc
     1681 catgatcttg accaatgtcg acttgccggc gccattttcg ccgacgacgg catgaacctc
     1741 cccggctttg aactccgcat cgacagaggt gagagcatgg acgccgtcga actgtttcga
     1801 gattccctct accttgagca agacctcact cccatgggtg ccggccggcc aaacctaatc
     1861 gcgggagtgg ggcatcgagg acc
//
LOCUS       DBNHXU010000414         3273 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_972098, whole genome shotgun sequence.
ACCESSION   DBNHXU010000414 DBNHXU010000000
VERSION     DBNHXU010000414.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_972098"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(59..1036)
                     /locus_tag="AC161X_10045"
     CDS             complement(59..1036)
                     /locus_tag="AC161X_10045"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013496082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HIR4989939.1"
                     /translation="MSVMNLAQSLNSALDTALESDDRVVLLGEDIGRTGGVFRITDGL
                     QEKYGENRVFDTPVAESGIVGAAFGMAIAGLRPVAEIQFLGFSYPAYDQVINHVARIR
                     NRSAHRFTAPLVIRIPFGGGIGAAEHHSESTEAIYTHIPGLKVVVPSTPHDAKGLLLA
                     AIEDPDPVIFLEPIRLYRAVKDEIPDIYYTSEIGPARIEREGKDITLISWGAMMKETR
                     AAATALGEHGVSVEVLDLRTLSPLDTATIVSSAEKTGRVVVIHEAPRTGGLGGEIAAI
                     IQEQAFYSLLAPVRRVTGWDTVFPLKRSEGHYLPNVDRIVQAAEQTLED"
     gene            complement(1033..2052)
                     /gene="pdhA"
                     /locus_tag="AC161X_10050"
     CDS             complement(1033..2052)
                     /gene="pdhA"
                     /locus_tag="AC161X_10050"
                     /inference="COORDINATES: protein motif:HMM:TIGR03181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045254 - pyruvate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0004739 - pyruvate dehydrogenase
                     (acetyl-transferring) activity [Evidence IEA];
                     GO_process: GO:0006086 - pyruvate decarboxylation to
                     acetyl-CoA [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HIR4989940.1"
                     /translation="MFSLINPDGTVRDRLPVTLEEMRALYADMVEARTYDTKCMAMQR
                     QGRLATYAPFEGQEAAQIGAAAAIRSDDWVAGTYRDAALMWRAGYPWELLIAGRTGDE
                     RGGKPPEGVNILPPSITVGGHMIHAVGLAWAERLQGSDRIALTSFGDGATSEGDFHEA
                     MNFAAVFKTPTVFFCQNNQFAISHPRSEQTMATSIAAKADGYGMPGVEVDGNDVAAVL
                     VAVREAAESARNGDGPALIEAVTYRLGPHTTSDDPGRYRDEAEADEWRERDPLIRIRI
                     LLDKAGGWTQEWQDELESRASAAIEAAVENAEALPLPTVDEMFERMFAEPTGSLRQQQ
                     DEAGS"
     gene            complement(2108..2893)
                     /locus_tag="AC161X_10055"
     CDS             complement(2108..2893)
                     /locus_tag="AC161X_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989941.1"
                     /translation="MNLVFTLGLTVAVAAAARSTWSPCGLSMLSTITPVTEQARGRRY
                     WATVPWYLLGALAGGATLGGLAALVSLPLGAYEIPEMTVFAIIGLFAVVSSFSDLGVK
                     GFRLPRHGRQVDRLWLDHYRSWVYGTGFGWQIGVGLATYIVTTAVYLTVLIAILTGSP
                     IQALIIGLVFGGLRGLAIFLGVRATTLDRLHALHRGLTRWEPQSRAVMFAIQAAVATL
                     AIGEAWGQIAKGVVFALLILVCLYMLRSGKTRPMAPNRTPNPV"
     gene            complement(2957..>3273)
                     /locus_tag="AC161X_10060"
     CDS             complement(2957..>3273)
                     /locus_tag="AC161X_10060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009405031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HIR4989942.1"
                     /translation="KEAVYQDQEVLEVVAAAAAAQADEEDDDDAALAREAMEMVVRSQ
                     LGSTSMLQRKLRIGFARAGRVMDMLERRGVVGPSQGSKAREVLMTVEELEEALAHQAV
                     EV"
BASE COUNT          625 a         1065 c         1009 g          574 t
ORIGIN      
        1 acgatttccg cttcggtgag cccttccccg atgtcgggaa gcctgaattc cttcgccact
       61 aatcctccaa ggtctgttcg gccgcctgaa cgatcctgtc cacattcgga aggtaatgac
      121 cctccgaacg tttcaaggga aacacggtgt cccacccggt gactcgtcgc actggcgcca
      181 gaagtgaata gaaggcctgc tcctggatga tcgcggcgat ctcgcctccg agcccaccgg
      241 tccttggcgc ctcgtggatc accacgaccc gaccggtctt ctcggccgac gacacgatcg
      301 tggcggtgtc gagcggtgac agcgtgcgga ggtcgagcac ttcaacggac acaccgtgct
      361 ccccgagagc cgtcgctgcg gcccgggtct ccttcatcat cgccccccag gagatgaggg
      421 tgatgtcctt gccctcacgc tcaattcggg caggcccgat ctcagacgtg tagtagatgt
      481 cagggatctc gtccttgacc gcccggtaga ggcgaatcgg ctccaggaag atcaccgggt
      541 cggggtcctc gatggccgcc agcaacaggc ccttggcgtc atgcggtgta gagggaacca
      601 ccactttgag tcccgggata tgggtgtaaa tggcttccgt cgactctgag tgatgctcgg
      661 cggccccaat tcccccacca aagggaatcc ggatgacgag aggcgcagtg aaccgatgcg
      721 cggagcggtt tcggatccgg gccacatggt tgatcacttg gtcataggcg ggataagaga
      781 aaccaaggaa ctggatctcg gcgaccgggc gcaggccggc gattgccatc ccgaaggccg
      841 cccccacgat cccggactcg gcaacgggag tgtcgaagac ccgattctcc ccatacttct
      901 cctggagacc gtcggtaatc cgaaacacgc caccggtgcg gccgatgtcc tcgccgagca
      961 ggaccacccg gtcatcggac tcgagagcgg tgtcgagtgc gctgttcaac gactgcgcca
     1021 gattcatgac gctcatgagc cggcctcgtc ctgctgctgg cgtagtgatc cggtgggttc
     1081 ggcgaacatg cgctcgaaca tctcgtcgac tgtgggcagg ggcagcgctt ccgcgttctc
     1141 gaccgccgcc tcgatggcag ccgaagcacg gctctccagt tcgtcctgcc attcctgggt
     1201 ccagccaccg gccttgtcga gaaggatccg gatccggatc agtgggtcgc gctctcgcca
     1261 ctcgtcggct tcggcctcgt cccgatatcg acccgggtcg tcggaggtgg tgtgcgggcc
     1321 gaggcggtaa gtgaccgcct cgatgagtgc gggcccgtca ccattgcggg ccgactcggc
     1381 cgcctcacga accgccacca ggacggcggc aacatcgttg ccatccacct ccacgcctgg
     1441 catcccgtac ccatcggcct tggccgcgat cgacgtggcc atggtctgtt ccgacctggg
     1501 gtgtgagatc gcaaactggt tgttctggca gaagaagacg gtcggagtct tgaaaaccgc
     1561 ggcgaagttc atcgcctcgt gaaagtcgcc ttcggaagtg gcgccgtctc cgaatgaggt
     1621 aagggcgatc cgatccgacc cttgaaggcg ctccgcccaa gccaggccca cagcgtggat
     1681 catgtgacca cccaccgtga tcgacggcgg caggatgttg accccctccg gcggtttgcc
     1741 ccctcgctcg tcaccggttc gacccgcgat cagaagctcc cacgggtagc ccgcccgcca
     1801 catcagggcg gcatccctat aggtccccgc cacccaatcg tcggagcgga tggcagcggc
     1861 ggcaccgatt tgggccgcct cctggccttc gaatggcgcg taagtggcga gacgaccctg
     1921 acgctgcatc gccatgcatt tcgtgtcgta ggtgcgcgcc tcgaccatgt ctgcatagag
     1981 agcgcgcatc tcctcgagag tcaccggaag gcgatcgcgg accgtcccgt cagggttgat
     2041 caggctgaac attcggcggc gagcctattc ggaggcggag agaacctgaa gagcagagtt
     2101 gtgtgggtca gaccgggttg ggagtccggt tcggtgccat cggccgggtt ttcccactcc
     2161 gaagcatgta gaggcacacc aaaatcaaca gggcaaacac cacgcctttg gctatctgac
     2221 cccatgcctc gccgatcgcc aaggtggcca ctgcagcctg aattgcaaac atgacggcgc
     2281 gagattgcgg ctcccaccgg gtgagaccac gatgaagcgc atgaagcctg tcgagggtgg
     2341 tggctcgcac gccgaggaat atggcgagtc cgcgcaaccc cccaaacacg aggccgatta
     2401 tgagtgcctg gatgggagag ccggtcaaaa tcgcaatcag gacagtcaga tagaccgcgg
     2461 tggtaacgat gtatgtggcc agcccgactc cgatctgcca accgaacccg gtgccataaa
     2521 cccaggatcg gtaatgatcc agccagagac ggtcaacctg acgaccgtgg cggggaagcc
     2581 gaaatccctt gactccaagg tcggagaagc tgctcaccac cgcgaacagc ccgatgatcg
     2641 caaagaccgt catctcggga atttcataag cccccaaggg cagcgatacc agcgcggcaa
     2701 gaccgcccag agtcgcacct ccagcgaggg caccgagcag gtaccagggg accgtggccc
     2761 aataccgccg acctcgcgcc tgctcggtga cgggggtgat ggtggagagc atcgatagcc
     2821 cgcaggggga ccacgttgag cgagcagcgg ctgcaacagc gactgtgaga ccgagagtga
     2881 aaaccaggtt catgtcagga gacctgggcc aaacctacca caggtccccg gatccgccat
     2941 tcacgcgacc gggtgctcag acctcaactg cctggtgggc cagggcctct tccagttcct
     3001 cgacagtcat caggacttcc cgtgccttgc tcccctggga ggggccgacg accccgcggc
     3061 gctcgagcat gtccatcact ctccccgccc gggcgaaccc gatccggagt ttgcgttgca
     3121 acatcgaagt ggagccgagc tggctgcgta ccaccatttc catcgcctct cgggccaggg
     3181 ctgcgtcgtc gtcgtcctct tcatcggcct gggcggctgc ggcagccgca accacttcga
     3241 ggacttcctg gtcctgatag acggcttcct tct
//
LOCUS       DBNHXU010000415         2636 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_5339441, whole genome shotgun sequence.
ACCESSION   DBNHXU010000415 DBNHXU010000000
VERSION     DBNHXU010000415.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2636)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2636
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_5339441"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="AC161X_10065"
     CDS             <1..250
                     /locus_tag="AC161X_10065"
                     /inference="COORDINATES: protein motif:HMM:NF024591.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP-binding enzyme"
                     /protein_id="HIR4989943.1"
                     /translation="IEAALDEIAGVESALVFGMPSPEWGMEVACVYVGSLVPEDVEVR
                     LRERLSGPLIPKRWHRVDALPTTDMGKPDRSQAARMLS"
     gene            complement(243..890)
                     /locus_tag="AC161X_10070"
     CDS             complement(243..890)
                     /locus_tag="AC161X_10070"
                     /EC_number="2.1.-.-"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.6"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008171 - O-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HIR4989944.1"
                     /translation="MTMTPERWDLLDDYSREVFGSEDDHLSGLMHEAVAAGLPDIAVT
                     ADVGRLLRILTSMTRGLLAVEVGTLAGYSGIWIARGLRPNGKLITIELDPKHAAFARE
                     QFARAGVADQVEIRLGKGIEVLTDLAAEIEPGSVDVLFIDAVKTEYSEYFQAARPLIA
                     VGGLVLADNVLGTSEGWVDQGYGTDDFNRLIAADPDFEAVAVPLRQGVMIARRFS"
     gene            993..1346
                     /locus_tag="AC161X_10075"
     CDS             993..1346
                     /locus_tag="AC161X_10075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989945.1"
                     /translation="MARSDEERIAELEESPQEVDLGEMEAEKIRLGALAKKTKRDRVI
                     DWGLGLFGGAVVGAIVAVNLIITVGIGYDVSLAEVYRENPLVGIVTTAILVAGPILGV
                     ALMRKLRRDRNRSTP"
     gene            1496..1903
                     /locus_tag="AC161X_10080"
     CDS             1496..1903
                     /locus_tag="AC161X_10080"
                     /inference="COORDINATES: protein motif:HMM:NF012791.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042802 - identical protein binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HIR4989946.1"
                     /translation="MIVVVGVDESSMAKEVLGRAIDEAAWREAELHVVNVYQLPFTYV
                     EIPVDYVAMAEGHHSAVWGPLEGQLSESRVELLRVDLEGYPAEALVSYANDIGASLLV
                     VGTRGRGEWGALILGSTSHRAVHLADCDVLIVR"
     gene            complement(1907..>2636)
                     /locus_tag="AC161X_10085"
     CDS             complement(1907..>2636)
                     /locus_tag="AC161X_10085"
                     /inference="COORDINATES: protein motif:HMM:NF022112.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="GerMN domain-containing protein"
                     /protein_id="HIR4989947.1"
                     /translation="VYFLLDEVGGSSGVGPFLVPVYREGPESDDAASDAVELLLAGPT
                     AGEMAGTPAMQTAIPEATSLLGLSVDDGVATVDLSAEFDDGGGSAGMFARLAQVVFTV
                     TRATGVDEVTFELASEPVAVFSSEGIELSRPQIRSDYYDWLPAIFVDMPAWGEPVTSP
                     IEVQGLSNVFEAVSQIMLTDDDGLPLFENAVMASCGTGCWGDWEASIPYEVDRHQVGA
                     LIVWEYSAKDGSRVNIREYPLLLR"
BASE COUNT          507 a          791 c          848 g          490 t
ORIGIN      
        1 gatcgaggct gccctggatg agatcgcagg agtcgagtcg gcgctggtgt tcgggatgcc
       61 cagtccggaa tgggggatgg aagtcgcctg tgtctacgtg gggagtttgg tgcctgaaga
      121 cgtcgaagtc cggctcagag agcggttgtc cggccccctg atcccgaagc gctggcatcg
      181 ggtagatgct ctgccgacta ctgacatggg caaacccgat cgcagccagg cggctcgaat
      241 gctcagctga aacggcgggc gatcatcaca ccctgacgca atgggaccgc gaccgcttcg
      301 aaatcggggt cggcggcgat cagccggttg aaatcgtcgg ttccgtatcc ctgatccacc
      361 cagccttcgc tggtaccgag gacgttgtcc gccagaacga gacccccgac cgcgatcaat
      421 ggtcgggcag cctggaaata ctcggagtac tccgttttca cggcgtcgat gaacaagacg
      481 tccacggacc cgggttctat ctccgccgcc agatcggtga gcacctcgat cccctttccg
      541 agtcggatct cgacctggtc agccaccccc gctcgtgcga actgttcccg ggcgaaggcg
      601 gcgtgcttcg gatccagctc gatcgtgatc agcttcccat tgggccggag accgcgggcg
      661 atccaaatcc ccgagtagcc ggccagggtg ccgacttcta ccgcgagcag acccctggtc
      721 atggaggtaa ggattctgag cagtcgtccc acatcggcag tcaccgcgat gtctggcagg
      781 ccggccgcca ctgcctcgtg catcagcccg gacaggtgat cgtcttcgga gccaaagacc
      841 tcacgggaat agtcgtcgag aagatcccaa cgctcgggtg tcattgtcat cgaccggacc
      901 ctagagcacc gtgtccgggt tggccgatcg aggctgcggg atcgggactt ccggctctgc
      961 cgagagtgag agaaggcatg catgctcggg gtatggcacg aagcgacgaa gagcgcattg
     1021 cggagttgga ggaatcgcct caggaggtgg atctggggga gatggaggcc gagaaaatca
     1081 gactcggggc tcttgccaag aaaaccaagc gtgaccgagt cattgactgg gggctgggcc
     1141 ttttcggtgg tgcagtagtc ggcgcgattg tcgctgtgaa cctcatcatc accgtcggga
     1201 ttggctatga cgtgagcctg gccgaggtct atcgggagaa cccgctggtg ggcatcgtca
     1261 ccaccgccat tctggtggcg ggcccaattc tgggcgtggc gctgatgcgg aagctgcgaa
     1321 gagaccggaa ccggtcgacg ccgtgacgag ggtttcccac cgcgatggct tcttcgcctc
     1381 gctgtggcct gaaaccgaac ctccgatgtg ggcagtacgg cgggtggacg gggacttccg
     1441 gccgtaggcg aaggccgacg gcccatgtca cccttgatcg aaccaaggag atcccgtgat
     1501 tgttgttgtc ggagtcgacg agtcgtcgat ggcgaaggag gtactgggga gggcaattga
     1561 tgaggcggcc tggcgggaag cggagcttca cgtcgtcaat gtctatcagt tgccattcac
     1621 ctacgtcgag ataccagtcg actatgtggc catggctgaa ggccaccaca gcgcggtgtg
     1681 gggcccgctc gaaggccaac tctccgagtc gagagtcgaa ttgctccgtg tcgatctcga
     1741 ggggtacccg gcggaggcgc ttgtgtcgta tgccaacgac attggcgctt cactattggt
     1801 ggtcggcact cgtggccgtg gcgagtgggg tgcgttgatc ctggggagca ccagccatcg
     1861 tgccgttcat ctggccgact gcgatgtgtt gatcgtcagg tgatcgtcac cgcagcagga
     1921 ggggatactc gcgaatgttg actcgacttc cgtctttggc cgagtactcc cagacgatca
     1981 gcgcaccgac ctggtgacga tccacttcgt aggggattga agcttcccaa tcgccccagc
     2041 atccggtgcc acagctggcc atgacggcat tctcaaacag gggcagccca tcgtcatccg
     2101 tgagcatgat ctgagaaacg gcctcgaaca cgttgctcaa cccctggacc tcgattggac
     2161 ttgttaccgg ctcaccccac gccggcatgt cgacgaagat ggccggcagc cagtcgtagt
     2221 agtcgcttct gatctggggg cgactgagct cgattccttc cgaggagaac acggcgactg
     2281 gctcgctcgc cagttcgaaa gtcacctcat cgacccccgt ggcgcgcgtc acggtgaaca
     2341 cgacctgagc aaggcgtgcg aacatgcccg cactgccacc gccgtcatcg aactcggcac
     2401 tgaggtcaac agtggcgaca ccgtcatcga cactcagccc gagcagtgag gttgcctcgg
     2461 ggatggcagt ttgcatcgcc ggggtcccag ccatttcacc tgcggtcggt cctgccagca
     2521 agagctccac agcatccgaa gccgcgtcgt ccgactcagg cccctcgcga tagaccggga
     2581 ccaggaacgg gccaacacca gagctcccac cgacctcatc caacaagaag tagacc
//
LOCUS       DBNHXU010000416         3622 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_379905, whole genome shotgun sequence.
ACCESSION   DBNHXU010000416 DBNHXU010000000
VERSION     DBNHXU010000416.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_379905"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..512
                     /locus_tag="AC161X_10090"
     CDS             <1..512
                     /locus_tag="AC161X_10090"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HIR4989948.1"
                     /translation="AGDRSAFAVLVREHGNEVYTLARRLVGDPHLAADVAQETMIRAW
                     RALPNFRGEAAFSTWLHRITVNTAWTQKKRARRHQGASLDDLAEVAAPYTREHPEVAG
                     EMIELRGRLRRALESLPESQRQVVILKDVYDWSHIEIAEAMGISVTAAKVRLHRARAR
                     LARNLEGPG"
     gene            509..841
                     /locus_tag="AC161X_10095"
     CDS             509..841
                     /locus_tag="AC161X_10095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989949.1"
                     /translation="MTIVCELAAPFVANSAVDNSDVIWPFRGHTDACLRCQARHAAMS
                     RTARELDGLSSETIRAPLDLEWRVMSSLEGDLAVARSWKRPIALAAAVLSMAAAIVIW
                     RLRPRAQS"
     gene            complement(845..1624)
                     /locus_tag="AC161X_10100"
     CDS             complement(845..1624)
                     /locus_tag="AC161X_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020543428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4989950.1"
                     /translation="MNDHVALITGANHGIGAATAKVLAAEGIGVLVTYLRLRDSADLP
                     EAYRANRAADAEQVVSAISAGGGKVIAIEADLADPAAPSMLFDTAEEALGSVDILINN
                     ATGWIADSFNGEDSDRFSRDLHQVSAATFDQQFAVDARATALLISEFAIRHRRHDLNW
                     GRLVGLTSGGPRGFPEEVSYGAAKAALENYSMSAALELADRGITSNVIHPPVTDTGWV
                     TDEVRKYVGGRVALPEDVAEVIAFLVSDQARLVTGNVIQMK"
     gene            1723..3279
                     /locus_tag="AC161X_10105"
     CDS             1723..3279
                     /locus_tag="AC161X_10105"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020523550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HIR4989951.1"
                     /translation="MFAIAQSGLIREVVAAMQAFTGLGSFDPVPSVEQSKAVSITVGR
                     KSPEAEFSGALISPGASLPDEVLVDWESLSAAGFAAGVGQTLILPQAERPTLVLVGAG
                     PGSDLDADKLRDAAAAFARATESSGNLELAIPDTGGVTPAVAAQVVVEGVLLARYRFD
                     ALKEGPTVTPITSLNLVVEGRRSAATKRGAAKGKTMSEATQMARDLANSPPAYLTASR
                     IAQVAVDIGPGRGLTIEVFDKDQLVEMGCGGLLGVNAGSSEPPRMVKLIYKPAKPRGH
                     LTLVGKGIMYDAGGIAIKPGDAVHATMKNDMSGAAAILAAMSELASLGCRAAVTGYLM
                     CTDNLPSGTAIAMGDVLKVRGGKTVEVANTDAEGRLVMADALVLAAEEGTDAIIDIAT
                     LTGAAMRALGTQVAGVIGNNAQMIEQVESAAAATGESVWELPLEHRYRGELNSDVADI
                     KNLGGPNAGAITAALFLEEFVAGLPWAHIDIAGTAQNDRDTSWRPPGCTGFGARLLIE
                     LALNFSRPRA"
     gene            3297..>3622
                     /locus_tag="AC161X_10110"
     CDS             3297..>3622
                     /locus_tag="AC161X_10110"
                     /inference="COORDINATES: protein motif:HMM:NF015746.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015558 - secondary active
                     p-aminobenzoyl-glutamate transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902604 - p-aminobenzoyl-glutamate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbgT family transporter"
                     /protein_id="HIR4989952.1"
                     /translation="MATTMEPAEKKSFSQKMLDGIEKAGNKVPHPVMMFAYLIVFIAV
                     LSALLAWAGVSVTEEIAVPVPNEVSEDAHGGLGGSDVPYDVTPMEPDFEPEYEIQEET
                     IEVQSLL"
BASE COUNT          664 a         1124 c         1180 g          654 t
ORIGIN      
        1 aggccgggga tcgctccgcc ttcgcagtcc tggtccgtga gcatggaaac gaggtctaca
       61 ccttggcccg caggctcgtt ggagatccac atctggctgc agatgtcgcc caggagacca
      121 tgattcgcgc ctggcgcgcc ctgcccaatt tccgaggcga agccgccttt tccacctggt
      181 tgcatcggat cacggtcaac accgcctgga cccagaagaa gcgggcccgt cgtcatcagg
      241 gggcctcact tgacgatctc gccgaggtgg ctgcccccta caccagggag catccggagg
      301 ttgctggtga gatgatcgag cttcgaggtc ggttgagacg ggctttggaa tccctccctg
      361 aatctcagcg acaggtggtg atcctcaaag acgtctacga ctggtcccac atcgagatcg
      421 cggaagcgat ggggatctca gtcaccgcgg ccaaagtccg tctgcaccgc gctcgggccc
      481 gtctggccag gaatctggag ggacctggat gaccatcgtg tgcgagctgg ctgctccttt
      541 cgtggcgaac agcgctgtcg acaacagcga tgtgatctgg cccttccgcg gccataccga
      601 cgcttgtctg cggtgccagg cgcgacacgc agctatgagt cgaaccgctc gggaactcga
      661 tggcctgtcc tcggagacga ttcgcgcccc gctggacctg gagtggaggg tgatgtcctc
      721 gctcgagggc gacctcgctg tagcccggtc ctggaagcgg ccgatcgcgc tcgcggccgc
      781 cgttctgtcg atggctgcgg ccatcgtcat ctggaggttg cggccccgcg cccagagttg
      841 aagctcattt catctggatc acgttgccgg tgaccaaccg ggcctggtcc gacacgagga
      901 aggcaatgac ctcggccacg tcttcgggta gagcgaccct gccgcccacg tacttcctca
      961 cttcgtcggt gacccatccg gtatcggtga ccggggggtg gatcacgttc gaggtgatgc
     1021 cccgatcggc caactccaaa gccgccgaca tcgagtagtt ctccagggca gccttggctg
     1081 ctccatagga cacctcttcg ggaaagccac gggggcctcc ggaggtcagc ccgaccagcc
     1141 gtccccagtt gaggtcgtgt cgacgatgcc gaatggcgaa ctccgagatc agtaaggctg
     1201 tcgcacgagc gtccactgcg aactgctggt cgaaggtggc cgccgacacc tggtgcaggt
     1261 ctcgactgaa gcggtcgctg tcctcgccgt tgaagctgtc tgcgatccac cctgttgcgt
     1321 tgttgatcaa gatgtcgacc gacccgagcg cttcttcggc ggtgtcgaac agcatcgatg
     1381 gcgccgccgg gtcggcaaga tccgcctcta tcgcgatcac tttgcccccg cccgccgaga
     1441 tggccgagac gacctgttca gcgtcggcag cccggttggc ccggtatgcc tcggggagat
     1501 cggccgaatc ccggagccgc aagtaggtca ccaggactcc aatcccttcc gcggccagga
     1561 ccttggcggt cgccgcccca attccgtggt tggcaccggt gatgagagcg acgtggtcgt
     1621 tcaagagcct cccaaatccg agtcccgagt cgcagggaga ctaggcacgc gcccgggtcg
     1681 tcctctgtag ttggaagatc cttgcgggct gatcgatctg atatgttcgc gatcgcccaa
     1741 tcgggcctga tcagagaggt tgtcgcggcg atgcaagcat tcactggact gggatccttc
     1801 gacccggtgc cctcggttga gcagtcgaag gcagtatcga tcaccgtggg gcgcaagtcg
     1861 cccgaggccg agttttcagg agccctgatc agtccgggag cgagtctccc cgatgaagtg
     1921 ctcgtcgatt gggagtcact atcggctgca gggttcgcgg ccggcgtggg ccagacattg
     1981 atccttcctc aggcggagag gccaaccctg gtacttgtgg gtgccgggcc ggggagcgat
     2041 ctcgacgccg acaagttacg ggacgctgct gcggctttcg cccgtgccac cgagtcgagt
     2101 ggcaatctgg aattggcgat cccggacacc ggaggcgtta ccccggcagt agcagcccag
     2161 gtggtggtcg agggagtcct ccttgcgcgc taccgctttg acgcgctcaa ggaaggcccg
     2221 accgtcacgc cgatcaccag cttgaacctg gtggttgaag gcagacgctc cgcggcgaca
     2281 aagaggggtg cagccaaggg caagaccatg tccgaggcga ctcaaatggc ccgggatctg
     2341 gccaattcgc cgccggcata tctcacggcg agtcgcattg ctcaggttgc tgtcgatatc
     2401 ggccctggcc ggggactcac cattgaggtg ttcgacaagg accagctcgt cgaaatggga
     2461 tgtggcggcc tgctcggggt caatgcaggg agcagcgagc cgccccgaat ggtgaagctg
     2521 atctataagc ctgcaaagcc gagaggacac ctcaccctgg taggcaaggg gatcatgtat
     2581 gacgccgggg gaatcgcgat caagcccgga gatgccgtcc acgcgaccat gaagaacgac
     2641 atgtcgggcg ccgcggccat cctcgctgcg atgtccgagc tggcctcgtt gggttgtcgg
     2701 gcggcggtga ccggttatct catgtgcacc gacaacctgc cgtcggggac cgccattgcc
     2761 atgggtgacg tgctcaaggt ccgcggcggg aagacggtcg aggtggccaa caccgacgcc
     2821 gaaggacgat tggtcatggc ggatgcactg gttctggctg ctgaggaggg gaccgacgcc
     2881 atcatcgaca tcgccaccct caccggagcc gcgatgcgag ccctcggcac ccaggtggcc
     2941 ggggtcatcg gcaacaacgc tcagatgatc gaacaggtcg aatcagcagc tgcggctact
     3001 ggcgagtcgg tgtgggaatt gccactggag caccgttatc ggggagagct caactcagac
     3061 gtggccgaca tcaagaacct gggtgggccc aacgccggtg caatcactgc cgccctgttc
     3121 cttgaagaat tcgtcgccgg gctgccgtgg gcccacatcg acatcgcagg gaccgctcag
     3181 aacgaccgag acacctcctg gcgcccaccg ggatgcaccg ggttcggcgc ccgtctcctc
     3241 atcgagcttg ctctgaactt ctcccgacct cgagcctgaa aggaacccca ggaagcatgg
     3301 ccacgacgat ggaaccggcg gagaagaaga gcttcagcca gaagatgctc gacggcatcg
     3361 agaaggcggg gaacaaggtc ccgcacccgg tgatgatgtt cgcctacctg attgtgttca
     3421 tcgccgtgct ctcggccctg ctcgcttggg ctggggtcag cgtcaccgag gagatcgcgg
     3481 ttccggtgcc aaacgaggtg tccgaagacg cacatggcgg attggggggc tccgatgtcc
     3541 cctatgacgt caccccaatg gagcccgatt tcgagcccga gtatgagatc caagaggaaa
     3601 ccatcgaggt gcagagcctt ct
//
LOCUS       DBNHXU010000417         2004 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1570404, whole genome shotgun sequence.
ACCESSION   DBNHXU010000417 DBNHXU010000000
VERSION     DBNHXU010000417.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2004)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2004
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1570404"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..733
                     /locus_tag="AC161X_10115"
     CDS             <1..733
                     /locus_tag="AC161X_10115"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycoside hydrolase 5 family protein"
                     /protein_id="HIR4989953.1"
                     /translation="EPDLFAHPNSAAEGRAWVREMTALIRDLDPDHQVTTGLHASNLF
                     VDNGLRVDQVFAESDVAVMHGYPMYVDWARDPLDPDFVPYLCALVTALSGMPCLAEEW
                     GGCTSPDTEESVVWEWTAYGSPRTQFMAGEGPFADYVGEVLPRLVDVGSTGAFLWCFA
                     DYAEDLWDRPPCDPGGAMHERHFGLVRPDGSLKPHAEVIRRFADSQPIVQQAAKTVTL
                     DITPDEYYADPWGHAQRLYESFLDR"
     gene            945..1952
                     /locus_tag="AC161X_10120"
     CDS             945..1952
                     /locus_tag="AC161X_10120"
                     /inference="COORDINATES: protein motif:HMM:NF012742.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HIR4989954.1"
                     /translation="MARTLDDLAELSGVSRATVSRVINGGAVSPETRQKVLDVLETTS
                     YRPNLAARNLASGKSGVVGVVMHVAAQLTFSDNYFAGLLTGICDSLTESAAGMMLWLG
                     NRTKEETLDQILSMGMLDGIIVTADTIDDPLVDGLRSSGVPSVLIGHRRADSDASYVD
                     IDNEAAGEAIADHLIGLGRQRIGHITGRRDSVSSRDRKTGYRRALRRANLPTEGLIIE
                     GDYTAEGGYKAATKLIEAKVDAIFCASDNTAVGALDAIRNAGLRVPDDIALAGFDDLA
                     FASELSPPLTTMRQDIQGIGQEAARNLLMLLDRPEGGPRRVLLPTELVIRQSTVGGLS
                     G"
BASE COUNT          375 a          657 c          608 g          364 t
ORIGIN      
        1 cgagcctgat ctgttcgcgc atcccaacag cgcggccgag ggccgggcgt gggtccgcga
       61 gatgaccgcc ctcatacgcg acctcgatcc ggatcatcag gtgaccaccg gcttgcacgc
      121 ttccaacttg ttcgtcgaca acggccttcg cgtcgaccag gtgttcgccg agagcgacgt
      181 ggcagtcatg catggctatc cgatgtacgt cgactgggct cgggacccct tggaccccga
      241 cttcgtgccc tacctctgtg ccctggtgac ggcactcagc gggatgccct gtctggcaga
      301 ggaatggggc gggtgcacgt ctcctgacac cgaagaatcg gtggtctggg aatggaccgc
      361 ctacggatca ccccgcactc agttcatggc aggggagggc cccttcgccg actacgtggg
      421 cgaggtactg ccccgactgg tggacgtcgg gtccaccggt gccttcctgt ggtgctttgc
      481 cgactacgca gaggatttgt gggaccgccc gccttgtgat ccgggtggag caatgcacga
      541 gcggcacttc ggcttggttc gccctgacgg aagcctaaag ccacacgccg aggtgattcg
      601 acgcttcgcc gactcccagc ccatcgtgca gcaagccgcg aaaaccgtca ctctggatat
      661 cacccccgac gagtactacg ccgatccctg gggtcacgcc caacgcttgt acgagtcgtt
      721 tctcgaccgc tgacgcggtc tctagccaca ggtcccaagt ctcaagtccc aaggctgaat
      781 ctggcatgcg gcctaggcct gttgcttgag actcgagacg ctggcgtagc cagccctgag
      841 agcgcttccc acgaattgcc cgaaaagttg atgccacttc ccttgacacg ctctaagaat
      901 tgtttgtatg tttgagagcg ctctcagagg aggagtctcc cggcatggca cggacgcttg
      961 atgacttggc cgaactcagc ggagtttctc gtgccaccgt ctcccgggtc atcaacggcg
     1021 gagcggtatc tcccgagacg cgacagaagg tcctcgatgt cctggaaacc accagctacc
     1081 ggccgaacct cgccgcacgc aacctcgcca gcggcaaatc cggcgtcgtc ggcgtcgtca
     1141 tgcatgtcgc ggcacagctc accttctcgg acaattactt tgctggcctg ctgaccggca
     1201 tctgcgactc gctcacagag tcagcggcgg ggatgatgct ctggctcggc aatcggacca
     1261 aggaagagac cctcgaccag atcctctcca tggggatgct cgacgggatc atcgtcaccg
     1321 ccgacaccat cgatgatccg ctggtcgacg gcctccgctc ctccggtgtg ccaagcgtgc
     1381 tcatcgggca ccgccgcgct gacagcgacg ccagctatgt cgacatcgac aacgaagctg
     1441 caggcgaggc gatcgcggat catttgatcg gactgggtcg acaacggatt ggccacatca
     1501 ccggcaggcg agacagtgtt tcgagtcgag atcgcaagac tggataccgg agggcgctgc
     1561 gccgggccaa tctccccacc gaaggcctga tcatcgaggg cgactacacc gccgaaggtg
     1621 gttacaaggc ggccaccaag ctgatcgagg ccaaggtcga tgccattttc tgcgccagtg
     1681 acaacacggc cgtcggggcg ctagatgcga tccgaaacgc cggattgcgc gtccccgacg
     1741 acatcgcact cgccgggttc gacgaccttg cgttcgccag cgagctctcc ccgccactca
     1801 cgacgatgcg ccaggacatc caggggatag gccaggaggc tgctcgcaac ctcctcatgc
     1861 tcctcgaccg ccccgagggc gggccacggc gggtacttct tccaaccgag ttggtgataa
     1921 ggcaatcaac ggttggaggt ctatcgggat agcccgaagt agcacggtcg cgcccgtccc
     1981 cggagtgcgg ccgcatcgat cgta
//
LOCUS       DBNHXU010000418         1524 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_972991, whole genome shotgun sequence.
ACCESSION   DBNHXU010000418 DBNHXU010000000
VERSION     DBNHXU010000418.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1524)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1524
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_972991"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="AC161X_10125"
     CDS             complement(<1..443)
                     /locus_tag="AC161X_10125"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018775187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0141098 - tRNA
                     (cytidine(34)-2'-O-ribose)-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (cytidine(34)-2'-O)-methyltransferase"
                     /protein_id="HIR4989955.1"
                     /translation="MFEIVFYHPEIPPNTGNAMRLAAVTGCRLHLIEPLGFTMDDAKL
                     RRAGMDYRDRASVTVHPDLDTWRGASAAPRVYALTVDGDQLHTDVAYEEGDAFLFGPE
                     SVGLPDEVKALPWITQRLRIPMVADVRSLNVANAAAVVVYEAWRQV"
     gene            complement(446..1114)
                     /gene="nucS"
                     /locus_tag="AC161X_10130"
     CDS             complement(446..1114)
                     /gene="nucS"
                     /locus_tag="AC161X_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016469832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease NucS"
                     /protein_id="HIR4989956.1"
                     /translation="MRLVIARCTVDYSGRLTAHLPEAVRLIMVKADGTLAIHSDGNAS
                     KPLNWMVAPNLLETFEDRWEVSNNKGEKLTIMIHEVIEDVIHSLGDDPGLQKDGVEAH
                     LQALLADRPWVLEEGLSLIRREFPTDIGPVDLLCADLTGSTVAIEVKRRGEIDGVEQL
                     ARYLERLENDPRLSPVRGILVAQVISPQARVLADARGIEAIEVDYDGLRDAEDTALRL
                     FDVR"
     gene            1176..>1524
                     /locus_tag="AC161X_10135"
     CDS             1176..>1524
                     /locus_tag="AC161X_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="exo-alpha-sialidase"
                     /protein_id="HIR4989957.1"
                     /translation="MTRIAVGTHKGAYVVEGNGKEWSVEGPLFPGWKATTFGAAPDGT
                     HITAVGSNWFGVGIQTSADFQVWEQTDSPPGWPDGGDRKMEQVWTIHNDGPRLWAGVS
                     EAGLFISDDQGQTW"
BASE COUNT          301 a          479 c          484 g          260 t
ORIGIN      
        1 acttgccgcc acgcctcgta gaccaccacc gcggccgcgt tggccacatt caatgagcgg
       61 acgtcggcga ccatcgggat cctcaaacgc tgtgtgatcc aggggagcgc cttgacttcg
      121 tcgggcaggc ccaccgactc gggcccgaag aggaaggcat cgccctcctc atatgccacg
      181 tcggtgtgca gttggtcacc gtcgactgtg agggcataca cccggggtgc agcagacgca
      241 ccccgccacg tgtcgagatc aggatggacc gtgaccgagg cgcgatcccg gtagtccatt
      301 ccggcccgtc tcagtttggc gtcgtccatc gtgaacccga ggggctcgat gagatggagc
      361 cggcacccgg tgaccgccgc caatctcatg gcgttgcccg tgttgggagg gatctccggg
      421 tgatagaaga cgatctcgaa cacggtcagc gaacgtcgaa caggcgaagg gcggtgtctt
      481 cggcgtcgcg taacccgtcg taatccacct cgatggcctc gatgcctcgt gcgtcggcaa
      541 gcacccgggc ctggggagag atgacctgcg cgacaaggat cccccgcacc ggcgacagac
      601 gagggtcgtt ctccaaacgc tccagatagc gggcgagttg ttcgacacca tcgatctcgc
      661 ccctccgctt gacctcgatc gcaacggtcg aaccggtcag gtcggcgcag agaagatcga
      721 cgggaccgat gtcggtgggg aactcacgcc ggatcaggga gaggccctcc tcgaggaccc
      781 agggacgatc agcgagcagc gcctgcaaat gggcctcgac gccgtccttc tgaagaccgg
      841 gatcatctcc caaggagtgg atgacgtcct cgatgacctc gtggatcatg atggtcagct
      901 tctccccctt gttgttgctc acttcccatc gatcctcgaa cgtctcaaga aggtttgggg
      961 cgaccatcca gttgagcggc ttggacgcat tgccgtcgga gtggatggcg agggtcccgt
     1021 cggctttgac catgatcagg cgcaccgcct cggggagatg ggcggtgagg cgacccgaat
     1081 agtcgacagt gcaacgggcg atgaccagcc tcacgagacc aacagggtac cgggcgctct
     1141 gacccactcc ctcgggccac taaattccaa tggtcatgac caggatcgca gtcggtactc
     1201 acaaaggggc ctacgtcgtc gagggcaacg gcaaggaatg gtctgtcgaa gggcctctct
     1261 tccccggctg gaaagccacc acatttggcg ccgcacctga cggaacccat atcaccgcag
     1321 tcgggagcaa ctggttcggc gtcggaatcc aaaccagtgc cgacttccaa gtgtgggagc
     1381 agaccgacag ccccccaggg tggcccgacg gtggggaccg gaagatggaa caggtctgga
     1441 ccatccacaa cgacggtccc cggctttggg ccggtgtctc tgaggccggc cttttcatat
     1501 ccgacgatca gggacaaacc tggg
//
LOCUS       DBNHXU010000419         3922 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1964128, whole genome shotgun sequence.
ACCESSION   DBNHXU010000419 DBNHXU010000000
VERSION     DBNHXU010000419.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 3922)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 3922)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3922
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1964128"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..867)
                     /locus_tag="AC161X_10140"
     CDS             complement(<1..867)
                     /locus_tag="AC161X_10140"
                     /inference="COORDINATES: protein motif:HMM:NF019850.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HIR4989958.1"
                     /translation="MIWALFVACVLAAIPAGLRWLRVAQREHYLAGSVTSFATRWWNS
                     GWLNRILGSVMCIGVVGFLAPWESLAWAFLVPAAQVGPVGLGIRGRTSRLAWTGRLRR
                     LAALSGLLFAVMVGIGVVFESGWVAAYALFLLPELIDLALWALAPLEKRLGQKWVDQA
                     ASRLRSSGVKVVAITGSYGKTTTKQYATHILSESFRTVASPASFNNRMGLARAVNEHL
                     VPGTEVFIAEMGTYGPGEIAALCEWVKPDISAIVAIGPVHLERFKTEERIVAAKSEIL
                     DGAAIGVIGIDHP"
     gene            complement(864..1610)
                     /locus_tag="AC161X_10145"
     CDS             complement(864..1610)
                     /locus_tag="AC161X_10145"
                     /inference="COORDINATES: protein motif:HMM:NF024109.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HIR4989959.1"
                     /translation="MALRVFANGALFADATGSGPPRVIALHGWARRGSDFRAVLGDLD
                     ALAPDLPGFGASPAPSEALGAEGYAHLVNELLDGCEVPPVVVGHSFGGRVAVCLAALY
                     PDRVGSLVLTGVPLLRVNPTRPPSARYRMLRAFNRSGMLSDEKFEEIRRQTGSSDYRA
                     ASGVMRDVLVRVVNESYEPQLRALTAPVSLIWGAGDREVPVSIAHAAAEVIESEGREV
                     DLQVLEGVGHFLPLEAPEALRAAIDRALAT"
     gene            1710..3458
                     /locus_tag="AC161X_10150"
     CDS             1710..3458
                     /locus_tag="AC161X_10150"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024583.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4989960.1"
                     /translation="MARFRASLLRRASAVAWVVLIIISAQAVRKEVFAQPAFWIPMSG
                     LSILLVFSTAARWDRLMATRAAPWIAAAWLMSLIVGITSLATIPELTATAQPILFGIA
                     AVSGLLLTWWAHALVTLFIAAAIFFIPYTMGEPDAFEQVLAPILTVAVVAAATALVGL
                     EFEREAVRSNERELQIDQQRLDFERLYAVSATLARAESLAEIVPHLIGTICKYLDAQV
                     GVVLLYDPDRHRLELMSPIWVNGYPLESEPITLDVAAAGIVTQTYRAGKPYYVREVDH
                     DSDNHILLKELGLKQALVAPLKVESLRVGVIIVGDPYVGTFTEDQVEQLGSLAAPAGL
                     VLSQIGRYEAQADMTKRLEEVAQMKSDFVSTVSHELRTPLTSIIGSLDTVNRPELQPT
                     TPTAQQLLSTARRQAGRLQRLIDDLLVVSRIDRGAIPVHLEPIGLRQAFDEVNRVVSI
                     EPTFVVEPEGLAVEADRDHLSRILINLVENAAKYAAGSPVELFAWERGPNVLIVVVDH
                     GPGIPDHERDRVFERFTQVDQSDTRSKGGTGLGLSIVKSLTEVMHGSVRVEATEGGGA
                     TFVVELPMAPALQTVD"
     gene            complement(3455..>3922)
                     /locus_tag="AC161X_10155"
     CDS             complement(3455..>3922)
                     /locus_tag="AC161X_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002635865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4989961.1"
                     /translation="ESAQTAFVTSAFSYKTLAVALLPLFDAAGGGALVTLDFDNSQAW
                     PAYDWMGVAKSALQSVTRYLARDLGPHGVRVNAVSAGPLTTVAAKGIPGFSVFDQVWN
                     ERSPLPWDTSNPDPVARMVCVLLSDWAPLTSGEIVHVDGGFHAMAAGVDKPES"
BASE COUNT          745 a         1316 c         1218 g          643 t
ORIGIN      
        1 tggatggtcg atgccgatca ccccgatcgc cgcaccgtcg aggatctccg atttggcggc
       61 aacgatccgc tcctcggtct tgaaacgctc cagatggacc gggccgatgg ccacgatggc
      121 ggagatatcg ggcttgaccc actcgcagag tgcggcgatc tcccccggcc cgtaagtccc
      181 catctcggcg atgaacacct cggtgcccgg cacgagatgc tcgttaaccg ctctcgccag
      241 ccccatccga ttgttgaacg atgccgggct ggcgacggtg cgaaacgact ccgacagaat
      301 gtgggttgcg tattgcttgg tggttgtctt gccataggag ccggtgatcg caaccacctt
      361 gaccccggac gaccgtagac ggctggcggc ctggtccacc cacttctgtc cgagacgctt
      421 ctccaaagga gcaagagccc acaaagccag gtcgatcagt tccgggagca ggaaaagcgc
      481 ataggcggcg acccagccag attcgaacac cacgccgatc ccgaccatca ctgcgaacag
      541 gagcccagac agcgccgcaa gtcgtcgcag cctcccggtc caggccagcc tggacgtccg
      601 ccccctgatc ccgagcccga ccggacccac ctgggccgcg ggcaccagaa atgcccaggc
      661 caggctctcc cacggcgcga ggaacccgac gaccccaatg cacatcaccg agcccagaat
      721 ccgattcagc catccactat tccaccaccg ggtggcgaac gaggtcaccg atccggccag
      781 gtagtgctct ctttgtgcga cacggagcca acgaagcccg gcgggaattg cggccaggac
      841 gcaggcgacg aacaaggccc agatcatgtg gcgagggccc ggtcgatggc ggcgcgcagg
      901 gcctcgggcg cctccagggg aaggaaatga cccacgccct cgaggacctg gagatcgact
      961 tcacgacctt ccgattcgat cacctcggcg gcagcgtgag cgatgctcac cggaacctcc
     1021 cggtcccccg ccccccagat aaggctcacc ggggcggtaa gggcccgaag ctggggctcg
     1081 tagctctcgt tgaccactcg aaccagcaca tcacgcatca ctccggacgc ggcgcggtag
     1141 tcggacgacc cggtctggcg ccggatctcc tcgaatttct catcactcag catcccggat
     1201 cggttgaagg cgcgcagcat ccggtaccgg gctgacggcg ggcgggttgg gttgacacgg
     1261 agcaagggga ccccggtgag caccagtgac ccgactcgat ccgggtacag ggcggcgaga
     1321 caaaccgcga cccggccccc aaacgagtgt cctacgacca caggcgggac ttcgcaccca
     1381 tcgagtagct cattgacgag atgggcgtag ccttcggcgc ctagggcctc ggaaggggcg
     1441 ggtgaggctc caaaaccggg taaatcgggc gccagagcat ccaaatcgcc aagcacggcg
     1501 cggaaatcac tgccccgccg tgcccatcca tgaagggcga ttacccgggg aggaccggaa
     1561 ccggtcgcat ctgcgaacag tgccccgttc gcgaatacgc gcaacgccac gtttcaatcc
     1621 tcgatcagtc cgggagaaat gccgagaata cagtcgaagc agttcgaaac catgacatgg
     1681 atgagctgaa gccaacgacc catcaagcga tggcccgttt ccgggcgtcg ctgcttcgcc
     1741 gcgcctcggc cgtggcctgg gtggtgctga tcatcatctc agcccaagcg gtccggaagg
     1801 aagtgttcgc ccagcctgcc ttctggatcc cgatgtccgg cctctcgatc ttgctcgtct
     1861 tttcaaccgc ggctcgttgg gatcgtctca tggcgactcg agccgccccc tggatcgctg
     1921 cggcgtggct gatgagcctc atcgtgggga tcacgagcct tgccactatc cccgagctga
     1981 ccgcaaccgc ccaaccgatc ttgttcggga tcgctgccgt ctccggtctt ctcctcacct
     2041 ggtgggccca tgccctggtg accctgttca tcgccgcggc catcttcttc atcccctaca
     2101 cgatgggaga gcccgacgcc ttcgaacagg tgctggcgcc gatcctgacc gtggccgtgg
     2161 tcgccgcagc caccgccctt gtcgggctcg agttcgagcg ggaggccgtc cggtccaacg
     2221 agcgggaact ccaaatcgac cagcagagac tcgacttcga gcgtctgtat gcggtgagcg
     2281 ccacccttgc tcgcgccgag tctctggccg agatcgttcc ccacctgatt gggacgatct
     2341 gcaagtacct cgacgcccag gtgggggtgg tcttgctcta cgacccggac cgtcatcgct
     2401 tggagctgat gagcccgatc tgggtcaacg ggtatccgct ggagtcagag ccgatcaccc
     2461 tcgacgtcgc cgcggccggg atcgtgaccc agacctatcg cgccggcaag ccctactacg
     2521 tccgggaagt tgaccatgac tcggacaacc acatcctcct caaggagctc ggcctgaagc
     2581 aggccctggt ggcgccgctg aaggtggaga gccttcgggt cggtgtgatc attgtcggcg
     2641 acccctatgt cggcaccttc accgaggacc aggtcgagca actcggatcg ttggccgccc
     2701 ctgcggggtt ggtgctctct cagatcggcc gatatgaggc gcaggcagat atgaccaaac
     2761 ggctcgaaga ggtggctcaa atgaagagcg acttcgtctc gacagtcagc cacgagctgc
     2821 gcaccccgct gacttcgatc atcggatcgt tggacaccgt caatcgcccg gagcttcagc
     2881 ccacaacgcc caccgcccag cagctcctgt caacggcgcg tcgtcaggcg ggtcgcctcc
     2941 agcgcctcat cgatgacctc ctggtggttt cccgaatcga ccggggagcg atcccggttc
     3001 atctggagcc gatcggcctc cgacaggcct tcgacgaggt gaaccgcgtg gtctcgatcg
     3061 agcccacgtt cgtcgtcgag cccgaaggcc tggcggtgga agccgaccgt gatcacctga
     3121 gccggatcct gatcaacctc gtggagaacg cagccaagta cgcggcagga agcccggtgg
     3181 agctattcgc ctgggaacga ggccccaatg ttctcatcgt ggtggtcgac catggccctg
     3241 gcatccccga ccacgaacgg gaccgcgtgt tcgagcgatt cacccaggtc gatcagtccg
     3301 acacgagatc caaggggggc accggtctag gcctgtcgat cgtgaagagc ctcaccgaag
     3361 tcatgcacgg ctcggtgcgg gtcgaggcaa ccgagggcgg tggggcgacc tttgtggtgg
     3421 agctcccaat ggcccctgcc ctacagacgg tcgactagct ctccggcttg tccaccccgg
     3481 cggccatcgc gtggaacccg ccgtcgacat ggacgatttc ccccgaggtc aggggagccc
     3541 agtcggagag gagcacacaa accatgcgag ccacggggtc agggttggag gtgtcccagg
     3601 gaagtggcga acgctcgttc cacacctgat cgaagacact gaacccggga atccctttgg
     3661 ccgccactgt ggtgagtggc cctgccgaca ccgcattgac ccgaaccccg tggggcccga
     3721 gatccctcgc cagatagcgg gtgacgctct gcagggccga cttggccacg cccatccagt
     3781 cataggccgg ccaggcctgg gagttgtcga agtcgagagt caccaaggct ccgccaccgg
     3841 ctgcgtcgaa gagcggaagg agagccacag ccagtgtctt gtaggaaaag gcggaggtga
     3901 cgaaggccgt ctgcgccgac tc
//
LOCUS       DBNHXU010000420         1707 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2364901, whole genome shotgun sequence.
ACCESSION   DBNHXU010000420 DBNHXU010000000
VERSION     DBNHXU010000420.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1707)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1707
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2364901"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            150..>1707
                     /locus_tag="AC161X_10160"
     CDS             150..>1707
                     /locus_tag="AC161X_10160"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006347143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HIR4989962.1"
                     /translation="MQAEWFYDDVPAESPLFADLNPTQREAVAATEGPVLVVAGAGSG
                     KTRVLTYRIAHLIRDLRVAPDAILAITFTNKAANEMRGRVGALVGGAVRSMQVSTFHS
                     ACVRILRREVSRLGYRSSFSIYDEADSIRLINLVLKDLDLDSKRFPAKAMKAVISKSK
                     NELVDFETFADQGEGFYHEQVADVYRLYQQRLLEASAMDFDDLLMVTVELFGAFPDVL
                     SQYQNKFRYVLVDEYQDTNRAQYVLVKQLTAVHRNLCVVGDSDQSIYRFRGADIRNIT
                     DFEKDYPDARIIVLDQNYRSSETILGAANSVIANNTKRTPKNLWSDKGPGVPIVRFEA
                     EDEHDEAGYVVDEIERLKSAGHNLSDMAVFYRTNAMSRVIEDVFVRRAIPYTVVGSVK
                     FYDRKEIKDAVAYLRTIVNPSDEVSVKRIINEPRRGIGDTTLAHVDRFSQTQGISFYE
                     GLRRAPEIHQLNGRAHAAVGEFVAIMDRLTESAAEGGVAAAVQSVLDDTSMLATLEAE
                     KTIEAMGRVEN"
BASE COUNT          337 a          529 c          537 g          304 t
ORIGIN      
        1 cgccgtcgca cggtggttga cgacgatgag cgacccgagg tccccgaaga ctcgagtcag
       61 gcggaagtcg atctcggcga tcccgtatac cgctcggctt gatcacaaca gtgtgattca
      121 gtcggtcgcg gccggtacca tcgccctaga tgcaggctga gtggttttac gacgacgtac
      181 cagccgagtc cccgctcttt gctgacctga acccgacgca acgcgaggca gtggctgcga
      241 ccgaagggcc ggtcctcgtt gtggcgggcg ccggttccgg caagacccgg gtgctcacct
      301 atcgcatcgc ccacttgatc cgcgacctcc gggtggcgcc agacgccatc ctggcgatca
      361 cctttaccaa caaggccgcg aacgagatga gagggcgggt gggagcgttg gttggcggcg
      421 ccgtccgctc gatgcaggtg tcgaccttcc actccgcctg tgtgcggatc ctgcgccgtg
      481 aggtctcgag gctgggctat cgctccagct tctcgatcta tgacgaagcg gactcgatcc
      541 ggctgatcaa cctggtcctc aaggacctcg atctggacag caagcggttt ccggccaagg
      601 cgatgaaggc ggtgatctcg aaatcgaaga acgagctggt cgacttcgag acctttgccg
      661 accagggtga gggcttctat cacgagcagg tcgcggatgt ctatcgcctc taccagcaga
      721 ggctcctcga ggcctcggcg atggacttcg acgaccttct gatggtgaca gtcgagctat
      781 ttggcgcctt ccccgacgtc ttgtcccagt accagaacaa gttccgatac gtgctggtcg
      841 acgagtacca ggacaccaat cgcgcccaat acgtcctggt gaagcagctc accgcggtcc
      901 atcgcaactt gtgcgtcgtc ggtgactccg accagtccat ctatcggttc cggggcgccg
      961 acatccggaa catcaccgac ttcgagaagg actacccgga cgccaggatc atcgttctcg
     1021 atcagaacta ccgctccagc gaaaccattc tcggcgcggc caactcggtc attgcgaaca
     1081 acaccaagcg cacacccaag aacctgtggt ccgacaaggg ccccggtgtg cccatcgttc
     1141 gattcgaggc cgaggacgag cacgatgaag ccgggtatgt ggtggacgag atcgaacgtc
     1201 ttaaatcggc cgggcacaac ctctcggaca tggcggtgtt ctatcggacc aatgccatgt
     1261 cgcgtgtgat cgaggacgtc ttcgtgcgac gggcgatccc atatacggtc gtgggctcgg
     1321 tcaagttcta cgatcggaag gagatcaagg acgcggtcgc ctatctgcgg acgatcgtca
     1381 acccctccga cgaggtgtcg gtgaagcgga tcatcaatga gccgaggcgg ggcatcggcg
     1441 ataccaccct ggcccacgtg gatcgcttca gccagaccca gggcatcagc ttctacgagg
     1501 ggctgcgcag ggcgccggag atccaccaat tgaacggccg agcgcacgcg gccgtaggcg
     1561 agttcgtcgc catcatggac cgtctcaccg agagcgcagc cgagggaggc gtcgcggccg
     1621 cggtgcagtc ggtgctcgac gacaccagca tgctggccac cctcgaagcc gagaagacga
     1681 tcgaggcgat ggggagggtg gagaacc
//
LOCUS       DBNHXU010000421         1890 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1973329, whole genome shotgun sequence.
ACCESSION   DBNHXU010000421 DBNHXU010000000
VERSION     DBNHXU010000421.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1890)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1890
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1973329"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..917
                     /locus_tag="AC161X_10165"
     CDS             48..917
                     /locus_tag="AC161X_10165"
                     /inference="COORDINATES: protein motif:HMM:NF025690.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4388 domain-containing protein"
                     /protein_id="HIR4989963.1"
                     /translation="MQLSGQLADWSVNDLLQIMQVTKKTGSLDVDGERRARVFFGEGR
                     VTGAELTGPKGSYLGGDRSGIADILYVLSGIETGTFSVGPADGPEIKGWTVEEILSDV
                     EALRSLEGEVIDAGLFEASGVRLIHEIDGPITIEPADWHALVTLVQPFTFNHLEVKFG
                     RGTAVRIFHTLQRLGMAEAVVAEDGESNWLDRLAEDVAPGTSAPAWLESSPEPEPAAT
                     ELVVEPAPAAESEPEPEPEPEHVLVSKAAEARSNSAKVAVEVKGVSAPASTTLTDGVY
                     DEIRRLRSRVADK"
     gene            929..1564
                     /gene="udk"
                     /locus_tag="AC161X_10170"
     CDS             929..1564
                     /gene="udk"
                     /locus_tag="AC161X_10170"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020402716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004849 - uridine kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HIR4989964.1"
                     /translation="MPERSPWKPDRPLFIGLAGGSGSGKTTIAESVLDRLDGQVALVH
                     HDAYYRHAPELSYEERTKVNYDHPASLETELLVEHLMALRSGVAIERPVYDFAVHLRS
                     DVTVRVEPAPVVVVEGILVLAEPELRSELDLKIFVDTDSDIRLARRLQRDIEERGRTV
                     DSVVSQYFETVRPMHIEYVETSKRYADLIIPEGYNPRAVATVVELIRSRLG"
BASE COUNT          354 a          609 c          596 g          331 t
ORIGIN      
        1 gccgatagtg cccaaaaggg acagggacct catctatgga atcacagatg cagctttcgg
       61 ggcaactcgc tgactggagc gtcaacgatc tgcttcagat catgcaggtc accaagaaga
      121 ctggctcttt ggatgtcgac ggggagcgca gagcccgcgt cttcttcggc gaaggaaggg
      181 tcaccggcgc cgagctgacc ggtcccaagg gcagctacct gggcggcgac cgcagcggca
      241 tcgccgacat cctctacgtc ttgtccggca tcgagaccgg cacgttctcc gtcgggcccg
      301 ccgatggccc ggagatcaaa ggatggacgg tcgaagaaat cctctctgac gtcgaagccc
      361 tcaggtccct ggagggagaa gtcattgacg ccggcctgtt cgaagccagc ggagtccgac
      421 tcattcatga gatcgacggc ccgatcacga tcgagcccgc cgactggcat gccctggtca
      481 ctctggtcca gcccttcacc ttcaaccacc tcgaggtgaa gtttgggcgg ggaaccgcag
      541 tgcgcatctt ccacacgctg caacggctcg ggatggccga ggcagtggtg gccgaagacg
      601 gtgagtcgaa ttggctcgat cgcctggccg aggacgtggc cccgggcacg agcgcccctg
      661 catggctgga aagctcgcct gagcccgaac cggcagcgac cgagctggtc gtcgagcccg
      721 caccggctgc cgagtcggag ccggagcccg agcctgagcc ggagcacgtg ctggtctcca
      781 aggccgcaga agcccgttcc aattccgcca aggtcgccgt cgaggtcaag ggggtttcgg
      841 cgccggcctc caccactctc accgacgggg tctacgacga gatccgccgt ctccgtagcc
      901 gggtagccga caagtaacgt cggcgggcat gcctgagcgc agcccctgga agccagatcg
      961 ccctctcttc atcgggcttg ccggagggtc gggatcgggc aagaccacga tcgccgaatc
     1021 ggtcctcgat cgtctcgacg gccaggtggc cctggttcac catgacgcct attaccgcca
     1081 cgcaccagag ctgagctacg aggagcggac caaggtcaac tacgaccacc cggcctcgtt
     1141 ggagaccgag ttgttggtcg aacatctcat ggccttgcgg tcaggcgtcg ccatagagcg
     1201 ccctgtatat gacttcgccg ttcacctccg ctcggacgtg acggtccggg tggaacctgc
     1261 gcccgttgtc gtggtggagg ggatcctcgt gcttgccgaa ccggagcttc ggtcagagct
     1321 cgacctgaag atcttcgttg acaccgattc cgacatccgt ctcgcccgtc gtctgcaaag
     1381 ggacatcgag gaacgggggc ggacagtgga ctcggtggtg agccagtact tcgagaccgt
     1441 gcgccccatg cacatcgaat atgtcgagac ctcgaagcgc tacgccgact tgatcatccc
     1501 cgagggctac aacccgaggg cggtggcgac agtggtcgag ctgatcagat cccgcctcgg
     1561 ctgagcatcc ggcccgtgcc gaaatcacca agaccacatt ccgacccgtc tcgtacctac
     1621 tcttcccgcc cagcgggttc cgcgacgtgg gaacgcagtt gcgttttgcg gcacacagag
     1681 gcaaggcaaa caggctggag cgtggattag cgacggtctt gtcggtggtt ttggccgccg
     1741 gagttctgtt gatcccgcaa caacccgccc aagcggcacc tgtcgattgc tcccgcgggg
     1801 tcaaccttgg attcgagacg ccgctggtgc gaagcaatcc catgggccca ttccccaact
     1861 gggccctgtt caacgaagca ttggttccgg
//
LOCUS       DBNHXU010000422        11147 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3760170, whole genome shotgun sequence.
ACCESSION   DBNHXU010000422 DBNHXU010000000
VERSION     DBNHXU010000422.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 11147)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11147)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11147
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3760170"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="AC161X_10175"
     CDS             complement(<1..644)
                     /locus_tag="AC161X_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018244990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HIR4989965.1"
                     /translation="MREGTKRFGGVLALDNVDVEVFAGEVLAILGDNGAGKSTLIKCI
                     SGVHRLDAGVIEMDGIEAAFHNPSDARELGIETVYQDLALFDNLSPSANFYAGREMAG
                     PGWLPTGMRFMRRRAMSVATREVLDRLHVGLPDLERPVGLMSGGQRQAVAVARATAFA
                     ARLVILDEPTAALGVREARGVLDLINRLRGEGKAVIVISHAMDHVIEVADRAVV"
     gene            complement(674..1606)
                     /locus_tag="AC161X_10180"
     CDS             complement(674..1606)
                     /locus_tag="AC161X_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HIR4989966.1"
                     /translation="MILAILILIIAVISPVFLTTRNIGNVLAQTAVIAVLALFQLLVI
                     VTRGIDLSVGSTLALATVVGALVYQSNGSGAGTIMIMLVTGLVVGLVNGFLYVKGRIP
                     HPFIVTLATLTLVRGVALWLSDGRPIQGAPEVVLTLGGKSMLGWIPVSTIVVVGTALL
                     VWLLTTRMVWGRWIYAVGGSPEAARRTAIPTSSVLISVYVLSGLAAGVAGVLTMGRTN
                     AGAPTFGALAELDAIAAVIIGGASFLGGRGTIGNALVGALMIGVIRNAMNLANLASFF
                     QLIVLGVVIILAVESDVLRGWLEQRVRLMQATRS"
     gene            1951..2682
                     /locus_tag="AC161X_10185"
     CDS             1951..2682
                     /locus_tag="AC161X_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018557526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HIR4989967.1"
                     /translation="MSAPLTEAAIRQIREMIASGALAPGAKLPSEADLAEDLGASRNT
                     LREAVRGLVTAGVLDVRRGDGTYVTSLRPEQLLEGIGAAAELMADGFSLELIQVRRIL
                     EPAATAMASLRIDEETISKLEIFLQRMRASETEEELVQVDSDFHAVVASASGNETLAS
                     MLNGVSTRTMRSRIWRGVIDADSKAQTIAQHAAILDALTRGDPVLAEAAALVHVATTE
                     DWFRRMIGGHVPDDRSRSRRSLPVA"
     gene            complement(2725..3489)
                     /gene="kduD"
                     /locus_tag="AC161X_10190"
     CDS             complement(2725..3489)
                     /gene="kduD"
                     /locus_tag="AC161X_10190"
                     /EC_number="1.1.1.127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007459157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000272 - polysaccharide catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-D-gluconate 5-dehydrogenase
                     KduD"
                     /protein_id="HIR4989968.1"
                     /translation="MPSAFDLQGKTALVTGAKRGLGMGIALGLARAGADIIGVSATQE
                     SEGSEIQGLVEQEGRSFVGRRCDFARRADTHELVDWLTANDHKVDILINNAGTIRRNP
                     ATDHTDEDWDQVLETNLSAPFVLTRGLAKAMIDRGRGKVVFIASMLSFQGGINVASYT
                     AAKSGIAGLTRALSNEWASQGVNVNAIAPGYMATDNTKALQDDPVRAKAIMERIPAGH
                     WGAPSDLAGAAVFLSSAASDYIHGAILTVDGGWLGR"
     gene            complement(3500..4261)
                     /locus_tag="AC161X_10195"
     CDS             complement(3500..4261)
                     /locus_tag="AC161X_10195"
                     /inference="COORDINATES: protein motif:HMM:NF013757.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HIR4989969.1"
                     /translation="MTAIDATPGTSAPAVVRAAAVLNLIAAAEGDPPRISELSRKLAL
                     PKSSTLNILSALTETGLVRRLGGGYALGPTLVDLASAFLRHEDPVQRFREFVPALATA
                     SQETVQLAMLNGPDVLYLARHDGNQPITLTSIVGKRLPATCTALGKVMLSALERPALE
                     EILVEPLRQLTLRSHATLESLIADLKATSERGYAIDDEEAAANVVCLAVAVPGRSGQG
                     AYAVSTTIFKDRLTPDLERPLVEDLTKLASYLSTI"
     gene            complement(4258..5199)
                     /locus_tag="AC161X_10200"
     CDS             complement(4258..5199)
                     /locus_tag="AC161X_10200"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009718253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-dependent alcohol dehydrogenase"
                     /protein_id="HIR4989970.1"
                     /translation="MQITVAYTGICGTDLKIAHGAMDNRITSPWPIGHEMSGIIAEVG
                     AGVEGWTEGDRVTVMPLDWCGQCPACEAGYSHVCHNLNFVGIDSPGSLQEKWNVRAGL
                     LVRIPSELDLRAAALVEPVAVAVHDVRRARLASKSQVVVLGGGPIGILIALVSRHHDA
                     QVIISEVNPFRAQFATGLGFHVINPRSEDLKTAVDEWTAGKGADFAFEVSGSSEGVAA
                     LTEVLAVRGRGVIVAIHPDPQPFNLFQMFWKELEIVGTRVYERTDFEEAIRLLTIGVI
                     PAKALITDVMPLSEASAAIERLSSGEDIMKLLVSSGE"
     gene            5346..6023
                     /locus_tag="AC161X_10205"
     CDS             5346..6023
                     /locus_tag="AC161X_10205"
                     /inference="COORDINATES: protein motif:HMM:NF013263.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta/alpha barrel domain-containing protein"
                     /protein_id="HIR4989971.1"
                     /translation="MAAHPKARILARISADGVVPVFYDGDIDLTKEVASRLVAGGLST
                     LEFTNRGDGAVAVFAQLVQWAHLELPALAIGMGSVIEAGTAGQAIDAGADFVFAPSLS
                     AEVAAVCHARNVPYIPGCGTVTEILAAYRLGCDLVKLFPAGSLGGPTFLSSIRAPCPW
                     IQAVPTGGVEPTVDSMKAWFDAGAPAVGMGSKLLPPQMIADEDWSGLQERIVATVAAV
                     KTARAGE"
     gene            6020..7126
                     /locus_tag="AC161X_10210"
     CDS             6020..7126
                     /locus_tag="AC161X_10210"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018113189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HIR4989972.1"
                     /translation="MSPVTLAIKSPESCRWDCVALGEVMLRLDPGESRVRTARSFRVS
                     EGGGEYNVARALSKVFALRTAVVTALVDNEVGHLVEDLMMTGGVDLRHVRWVPHDGIG
                     LESRVGLNFTEKGYGIRPALGVSDRAHTAVSQLRPGDIDWHQIFDVEGVRWLHTGGIF
                     AALSEGTAEVALAAMQAAKASGTAVSYDLNYRPSLWKPRGGQAAATEVNRRLARYVDV
                     MIGNEEDFTAALGIEVEGVDEEILTIDTSSFETMIGRAVEEFPDFAVVATTLRVAKSA
                     GFNDWGAILFADGEFHHARVREDLQIYDRVGGGDGFASGVIWSFLEGRSAEEAVEIGA
                     AHGALTMTTPGDTSMMTASEVLRAVSAQGARIVR"
     gene            complement(7140..8315)
                     /locus_tag="AC161X_10215"
     CDS             complement(7140..8315)
                     /locus_tag="AC161X_10215"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014414.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HIR4989973.1"
                     /translation="MTPMMACGLWIFRRLAREGALEVEMPTGDRVTIGHGHPIADISI
                     RDLGAILAILREGLNGFAEAYMSGRIDTSDLDRLIAWGLANQKAWFDHPLAVATSPIR
                     RVWQRIRPDRRHSRVRTMNEHYNLGNDFYASWLDPTMTYSSARFQSPEQILEEAQRHK
                     YRTIAEHAQLAPGMRVLEVGCGWGGFAEYAAIEIGCDLVGITLAKEHAGYAEKRIANA
                     GLADRVEIRIEDFRSTTGTFDAVVSIEMIESIDERQWPDLFQTIFARLEPGALAAMQI
                     ITIADPYWERYRSRSDFIQQYIFPSGQLPAPKVLRRLSADAGLEVEQIETFGPDYART
                     LAAWRANFTESWDRIQSEHDLDERFRRMWHLYLTLCEAGFTLGRINVEQWVFRKPAS"
     gene            complement(8312..9094)
                     /locus_tag="AC161X_10220"
     CDS             complement(8312..9094)
                     /locus_tag="AC161X_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007674957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1365 domain-containing protein"
                     /protein_id="HIR4989974.1"
                     /translation="MSGFHSALYRGTVRHTRLRPVHHDFKYRVYYGLWDIDELDRLDR
                     SLRLFSLDRFNLFSIDRRDHGAEDGTSLRAWVVKILARAGVELNGGRIDLLAYPRVLG
                     YAFNPISVWYCYDLENRLMAVVHEVRNTFGDKHSYVVPIDGRDLSHEFEKHLHVSPFN
                     DMDSNYSFSMTRPGEHLSLGISQGDDNGHFLRAGLSLTRIPLTDLNLMRLFWSHPLLT
                     LKVVGGIHWEAVRLWFKGARFHRRPMPPLDSVTVVDTFRSVR"
     gene            complement(9091..10401)
                     /locus_tag="AC161X_10225"
     CDS             complement(9091..10401)
                     /locus_tag="AC161X_10225"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018431230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HIR4989975.1"
                     /translation="MGQRIAVVGAGISGLGAAWALHRDNEIVVYEADERLGGHSNTVE
                     VETPSGAVPVDTGFIVYNEVTYPHLTRLFSALGVNTEGSDMSFAFSMGKSFEYAASAR
                     GLVAQPTNLLRASYRSMISDIMRFRRIGSELRPRQDETIDELLTRHGFGAAFLEEYLL
                     PMTASIWSARQSEIRKFPAVSILRFLNNHGLIRITDRPLWRTVSGGSREYVRRLAHPF
                     KDRVRVATPVTLVERHEDRVEITANGRRESFDQLVLATHSDHSLAILGRNATDTERNV
                     LGEIRYEPNHAVLHSDPSLMPTRRRVWSSWNAMVSPADRGSRVASVTYWMNRLQRLDT
                     GKQLFVSLNPIQDPDPSLTHRRFDYAHPQFDHRTVDAQRRLAKIQGARRTWFAGAYCG
                     YGFHEDGLQAGLNVAAALGSPPPWWGSFEPMSSSLPPDRIGALV"
     gene            10493..10921
                     /locus_tag="AC161X_10230"
     CDS             10493..10921
                     /locus_tag="AC161X_10230"
                     /inference="COORDINATES: protein motif:HMM:NF022072.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HIR4989976.1"
                     /translation="MRLQEQRTVLRGLDEVFAYTADFSNIENWDPGVARSAKVGVEAV
                     GVGTRFDLDVKFGSRIVPMTYEVTVYEPPKRVVLVGTGDTVGAIDEIRFSSQNGRTII
                     DYTADIEFRNFIRFLTPFMGSTMRKVGERALDGLVTALDG"
     gene            10918..>11147
                     /locus_tag="AC161X_10235"
     CDS             10918..>11147
                     /locus_tag="AC161X_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019633633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HIR4989977.1"
                     /translation="MNKLSIAGFTNLGFQLHSRSFEPVGADLTSKTVLITGASGGLGE
                     AATVALTGLGARVLAVGRDRAKLDAVEQRTAGR"
BASE COUNT         2114 a         3341 c         3558 g         2134 t
ORIGIN      
        1 atcaccacag ctcggtcggc gacctcgatc acgtggtcca tcgcatggga aatgacgatt
       61 accgccttgc cctcgccgcg taaccggttg attaggtcga ggacacctcg ggcttcgcga
      121 acgccaagtg cggccgtcgg ctcgtcgagg atcaccagcc gtgccgcgaa cgccgtcgcg
      181 cgagccacgg ccactgcctg acgctgaccg ccggacatca aacccaccgg gcgctcgagg
      241 tctggcagac cgacatgcaa gcgatcgagc acctctctgg ttgcaacaga catggctcgt
      301 cgtcgcatga aacgcattcc ggtcggaagc catcccggcc cagccatctc ccgacccgcg
      361 tagaagttcg ccgaagggct gaggttgtcg aagagtgcaa gatcctgata gacggtctcg
      421 atgccgagtt ccctggcgtc cgacgggttg tggaacgcgg cctcgattcc gtccatctcg
      481 atcacgcccg cgtcgagccg gtggacaccg ctgatgcact tgataagtgt cgatttgcca
      541 gcaccgttgt caccgaggat cgccagaact tccccggcga agacctcgac atcgacgttg
      601 tcgagggcaa gtactccacc gaaccttttg gtgccctctc gcactctcaa aacgggttcg
      661 agtccttgtg gcgtcacgag cgtgttgcct gcatcagccg gacgcgctgc tcgagccagc
      721 cgcgaagaac atcggactca acggcgagga tgatgaccac gcccaacacg atgagttgga
      781 agaaggaggc gaggttggcc aggttcatcg cgtttcggat caccccgatc atcagcgccc
      841 ccacgagggc atttccgatc gtgccccggc cacccagaaa gctggctccg ccaatgatca
      901 ccgcagcgat ggcgtcgagc tcggccagtg ccccaaacgt cggagcgccg gcgttggtcc
      961 ggcccattgt gaggacgccg gccacccccg cggccagccc actcagcacg tagaccgaga
     1021 tcagcaccga agacgttgga atggcagttc ggcgtgctgc ttccgggctg cctccaaccg
     1081 catagatcca acgtccccaa accattcggg tcgtgagcag ccagacaagg agggcggtgc
     1141 caacgaccac gattgtcgaa accggaatcc agccgagcat cgactttcct ccgagggtca
     1201 gaacaacctc aggcgctccc tggatcgggc gcccgtcgga gagccagagc gcgacgcccc
     1261 gcaccagcgt caaggtggcc agcgtcacga tgaacggatg cgggatcctg cccttgacgt
     1321 agaggaatcc attgacgagg ccgacaacaa gtccggtgac cagcatgatc atgatggttc
     1381 ccgcccctga gccattggac tggtagacga gagcgccaac gaccgtcgcc aaggccagcg
     1441 tcgatccaac cgataaatcg attcctcttg tgactatgac cagcagttgg aagagagcca
     1501 gcaccgcgat cacagcggtc tgggcgagca cgttgccgat gttgcgcgtt gtcagaaaga
     1561 ccggactgat cacagcgatg atgagaatga ggatggcgag gatcaggacc ggtcccgctt
     1621 tcagaaacca caacgccaac cccagagatc gatcgaatct ggctctttcc actatctcag
     1681 tggcttcggg atcggatgaa tctgtaggac tctcgggatg ggacgcattc gcggcggtct
     1741 ttccggcgct ggggccagtg ttacggagat caggctccca tgatccggct cccgtcctgt
     1801 cacccacttc gatcctccac cttcgaatgc atgccagcga tgagagtgat cgggtgtcta
     1861 acgccaaatt ggtcactctc ggacccgccg gggtagcata cgggcttaaa cataggatgt
     1921 ctagttgtca aacaggagga gaacgaggac atgtcggcgc ccctgactga ggcggcgatt
     1981 cgccaaattc gcgagatgat cgccagtggc gccttggcgc ccggggccaa gctcccgtcg
     2041 gaggccgacc tcgcggagga tctgggagca tccaggaaca cgttgcgtga ggctgtgcga
     2101 gggctggtca ccgcaggggt tctcgatgtt cggcggggag atgggacgta tgtcaccagt
     2161 ctgcgcccgg agcagctcct ggagggcatc ggtgcggcag ccgagttgat ggccgatggc
     2221 ttttcgctcg aactgatcca ggttcgtcgg attctcgaac ctgccgccac cgcgatggcc
     2281 tcgctgcgca tcgacgaaga gacgatctcc aagctggaga tattcctgca gagaatgcgg
     2341 gcttcggaga cagaggaaga gctggtccag gtcgacagcg actttcacgc tgttgtcgcc
     2401 tctgcttccg gaaatgagac cctggcatcg atgctgaacg gggtatccac taggacgatg
     2461 cgttcgagaa tctggcgcgg tgtcatcgat gccgattcca aggcacagac cattgcccaa
     2521 catgccgcaa tcctcgacgc actgactcga ggcgatccgg tgttggccga agccgcagcg
     2581 ctggtccacg tcgccacgac tgaagactgg ttccggcgca tgatcggcgg gcacgtgcct
     2641 gatgaccgca gcagatcccg gcgctcgctg cctgtggcgt aaagggctcc gcggactcgg
     2701 actcggactt ggggtcgggt ccggctatcg gcccagccag ccgccatcca cggtgaggat
     2761 tgcgccatgg atgtagtcgg aggcagccga tgaaaggaag accgctgctc ccgccaggtc
     2821 ggacggtgct ccccagtgcc cggcaggaat tcgctccatt atcgccttgg cccggaccgg
     2881 atcgtcctgg agcgctttgg tgttgtccgt cgccatgtat ccaggggcga tggcgttgac
     2941 attgactcct tgggacgccc actcgttgga caacgctctg gtgaggccgg cgatcccact
     3001 cttggccgcg gtatagctgg ccacgttgat accaccctgg aagctcaaca tcgaagctat
     3061 gaaaacgact ttgcctcgcc cacggtcgat catcgccttc gcgagccctc gggtgaggac
     3121 gaacggagca ctcaggttgg tctccaggac ctggtcccag tcttcatcgg tatggtcggt
     3181 ggcggggttg cggcgaatgg tcccggcgtt gttgatcagg atgtcgacct tgtggtcgtt
     3241 ggcggtgagc caatccacaa gctcatgggt atcggcgcgt cgggcaaagt cgcatctgcg
     3301 gccaacaaag gatcgtccct cttgctcgac cagtccctgg atctctgagc cctctgactc
     3361 ctgtgtcgcc gacaccccga tgatgtcagc gccggcacgt gccaatccga gggcaattcc
     3421 catccccaaa cctcgtttgg caccggtgac aagggcggtc ttgccttgga gatcgaaagc
     3481 gcttggcatc ttgtgttgct cagatggtcg agaggtagct ggcgagtttg gttaggtcct
     3541 cgaccagcgg ccgctccaaa tcaggggtga gccggtcctt aaagatcgtg gtgctgacgg
     3601 cataagcccc ctgaccgctt cgtccgggga cggcgacagc gaggcaaacc acgttggcgg
     3661 cggcctcctc gtcatcgatg gcgtatccac gctccgatgt tgccttaagg tcagcgatca
     3721 ggctttcgag agtggcgtgt gaccgcaggg tgagctgtcg aagcggctcg accaatatct
     3781 cctcgagggc gggccgctcg agagcgctca gcatcacctt gcctaatgcg gtgcacgtcg
     3841 caggcagacg tttgccgacg atcgaagtca gggtgatcgg ttggttgccg tcgtgtcggg
     3901 cgaggtagag gacatcgggg ccattcaaca tcgccaactg tacggtctcc tgagaggcgg
     3961 tggccagggc gggcacgaat tcccgaaacc tctgaacggg atcctcgtga cggagaaatg
     4021 cagacgccag gtcgaccaac gttggcccga gtgcgtagcc cccaccaagc cggcggacga
     4081 gacccgtctc agtcagtgcc gacaggatgt tcaaggttga tgacttgggc agtgcgagct
     4141 tccgggacaa ctcgctgatc cggggcgggt cgccttcggc ggcggcgatc aggttcaaga
     4201 ccgctgcggc gcgaacgact gccggcgccg aggtcccggg cgttgcgtca atcgccgtca
     4261 ttcacctgag ctcacgagca acttcatgat gtcttcgccg cttgaaagcc gttcgatggc
     4321 agccgatgcc tcggagagcg gcatcacatc agtgatcagc gcctttgcgg gtatgactcc
     4381 gatggtcagg agacggatag cttcctcgaa gtcggtccgc tcgtacaccc gggtgccgac
     4441 aatctcgagc tccttccaaa acatctggaa caagttgaaa ggctggggat cgggatgaat
     4501 cgcaacgatc acaccccgcc cccggactgc caggacctcg gtcaaggcgg ctacgccctc
     4561 agaagaccct gagacctcga atgcgaaatc ggcccctttg cctgcagtcc attcgtcgac
     4621 cgcggtcttc aggtcctcgg atctggggtt gattacatga aagccgagcc cggtcgcgaa
     4681 ctgggcccgg aacgggttga cctccgagat gatgacctgg gcgtcgtggt gtcgggacac
     4741 gagtgcgatg aggatgccga tcgggcctcc cccgaggacc acaacctggc ttttgcttgc
     4801 caagcgagca cggcgcacat cgtggaccgc gaccgccact ggctcgacca gagcagcggc
     4861 tcgcagatcg agttcagagg ggatgcggac gagcaggccg gcccgcacgt tccatttctc
     4921 ttgcaatgat cccggggagt cgatcccgac aaagttgagg ttatggcaga cgtgcgagta
     4981 tccggcttcg cacgcggggc attgcccgca ccagtccagt ggcatcaccg tgaccctgtc
     5041 cccttcggtc cagccctcca cgccggcacc cacctcggcg atgattcccg acatctcgtg
     5101 gccgataggc cagggcgagg tgatccggtt gtccatggcg ccgtgagcaa ttttcaggtc
     5161 ggtgccgcag ataccggtat aggcgaccgt gatctgcact tcgccgtcac caggtcgcgc
     5221 gaccggaagg cgtatcgctt cgaactcccc gttctggtgg tattggacta ctagttggtc
     5281 gatgatgcgg gcccctgatg ttcagtagag tgtaatgtcg ccagcatact gaacaggagg
     5341 tgctggtggc ggctcacccc aaggccagga ttctggcgag aatcagtgct gacggcgtgg
     5401 tgccagtctt ctatgacggc gacatcgatt tgacgaaaga ggtcgctagc cggctggttg
     5461 ctggtggtct ctcgacactg gagttcacca atcgcggcga cggcgcggtc gctgtcttcg
     5521 cccagcttgt gcagtgggcg cacttggaat tgccggcgct tgcgattggc atgggatcgg
     5581 tgatcgaggc aggcaccgcg ggtcaggcga tcgacgccgg cgccgatttc gtgttcgccc
     5641 cctcactctc ggccgaggta gcagcggtgt gccacgcccg aaatgtcccc tacatcccgg
     5701 gttgtgggac tgtcaccgag atcctggctg cttaccgctt gggctgcgac ctggtcaagt
     5761 tgtttcccgc cgggtcgctc gggggtccga cctttcttag ctccatcagg gcgccctgcc
     5821 cctggataca ggctgttccg acaggtggag tcgagccgac tgtggattcg atgaaagcat
     5881 ggttcgacgc cggggccccg gcggtgggaa tgggctcgaa gctgttgccc ccccagatga
     5941 tcgcggatga ggattggagt ggcctccagg agagaatcgt ggccacggtt gccgctgtga
     6001 agacggctcg ggccggggag tgagcccagt gaccctggca atcaagagcc ccgagagctg
     6061 tcgatgggat tgtgtggctc tcggtgaggt gatgcttcga ctggatcccg gagaaagcag
     6121 ggtgcgaact gcgcgctcgt ttcgggtgag tgaaggggga ggggagtaca acgtcgcccg
     6181 ggcgctgagc aaggtgtttg ctctacggac tgctgtggtc accgcactcg tcgacaacga
     6241 ggtcggccac ctggtggagg acctgatgat gaccggaggc gtcgacctcc gacatgtgcg
     6301 gtgggtgccc catgatggga tcggtctcga atcccgtgtt ggcctgaact tcacggagaa
     6361 gggctatggg attcgtccgg cgctcggcgt gtcagaccgg gcccacaccg cagtctctca
     6421 gctaaggccg ggggacatcg attggcacca gatcttcgac gttgagggcg ttcgctggct
     6481 gcacacaggt gggatcttcg cggccctatc tgagggtacg gccgaggtcg ccctcgcggc
     6541 aatgcaggcc gccaaggcgt cgggcacggc cgtttcctac gacctgaact atcgcccttc
     6601 cctttggaag ccccgcggtg gccaggctgc ggcgacggaa gtcaatcggc gtcttgcccg
     6661 atacgtcgat gtgatgatcg gcaatgagga ggacttcaca gccgcgctcg ggatcgaggt
     6721 cgaaggagtc gacgaggaaa tcttgaccat cgacacctcc agcttcgaaa ccatgatcgg
     6781 tcgagcggtc gaggaattcc cggacttcgc agtggtcgca accacgcttc gtgttgccaa
     6841 gagcgcaggg ttcaacgatt ggggagcgat cctctttgct gacggcgagt ttcatcacgc
     6901 tcgagtgcgg gaggatcttc agatctatga ccgggtcggc gggggagatg gatttgccag
     6961 cggggtgatc tggagcttcc tcgaaggaag gagcgcggag gaggccgtcg agatcggagc
     7021 cgctcacgga gcgctaacga tgacgacgcc aggcgacacg tcgatgatga ccgcctccga
     7081 agtgctcaga gcggtatcag ctcaaggagc gcgcatcgtc aggtgagtac gacgatggct
     7141 catgaggccg gcttgcggaa cacccactgc tcgacattga tccggcccaa ggtgaaaccg
     7201 gcctcgcaaa gggtcaagta gaggtgccac atccggcgaa acctctcgtc gaggtcgtgc
     7261 tcgctctgga tgcgatccca ggactcagtg aagttcgctc tccatgcggc gagggttcgg
     7321 gcgtagtcgg ggccgaacgt ctcgatctgc tccacctcga gaccggcatc ggcgctgagg
     7381 cggcgaagca ccttgggggc cggcaactgt ccgctcggaa atatgtactg ctggatgaag
     7441 tctgatctac tccgatatcg ctcccagtag gggtcggcga tggtgatgat ctgcatcgcg
     7501 gccagggctc ccggttccag gcgtgcgaaa atggtctgga agagatcagg ccactgcctc
     7561 tcgtcgatcg attcgatcat ctcgattgag accacggcat cgaaggtccc ggtggtgctc
     7621 cggaagtcct cgatccggat ttcaactctg tcggcaaggc cggcgttggc gatgcgcttc
     7681 tctgcgtacc cggcgtgctc cttggcgagc gtgattccaa ccaaatcaca gccgatctcg
     7741 atcgccgcgt actcggcgaa ccctccccag ccgcatccaa cctcgagcac tctcatcccg
     7801 ggagcgagtt gagcgtgctc ggcgatcgtt cggtacttgt gccgttgtgc ctcttccaga
     7861 atctgttctg gactttggaa ccgggccgat gagtaggtca tcgtcgggtc cagccaggat
     7921 gcgtagaagt cgttgcccag gttgtagtgc tcgttcatgg tgcgcactcg tgagtggcgc
     7981 cggtcgggtc gaatccgctg ccatacccgc cggatcggac tggtcgcgac tgccagtgga
     8041 tggtcgaacc acgccttctg gttggcgaga ccccaggcga tgagtcggtc cagatcgctg
     8101 gtgtcgatcc gcccgctcat gtacgcctca gcgaatccat tgagcccttc tcgaaggatc
     8161 gccaggatcg ccccgaggtc tcggatcgaa atgtcggcga tgggatggcc gtggccgatt
     8221 gttacccgat ctccggtcgg catctcgacc tcgagggctc cctccctcgc cagacggcgg
     8281 aagatccaca aaccgcatgc catcatcggt gtcatcgcac actcctgaag gtgtcgacca
     8341 ccgtgacgct atctaacggc ggcatgggac gtctgtggaa tctggcaccc ttgaaccaaa
     8401 ggcgaactgc ttcccagtga atcccgccga ccaccttcag ggtgagcaga gggtggctcc
     8461 agaacagtcg catcaggttg aggtcggtga gagggatgcg ggtcagggag aggccagccc
     8521 gtaggaagtg gccgttgtcg tcgccttgac tgattcccaa agacaagtgc tctccaggac
     8581 gggtcattga gaacgagtag ttcgagtcca tgtcgttgaa cggcgagacg tggaggtgct
     8641 tctcgaactc gtgactcagg tcacggccgt cgatgggcac gacataggag tgcttgtccc
     8701 cgaaggtgtt cctgacttca tggaccaccg ccatgagccg gttctcgagg tcgtagcaat
     8761 accagacgct gatcgggttg aaggcatatc cgagcactct gggataggcg aggaggtcga
     8821 tccgcccccc gttgagctcg actcctgccc gagccaggat cttcaccacc caggctcgca
     8881 aggacgtccc gtcctccgct ccatggtcgc gcctgtcgat cgagaagaga ttgaagcggt
     8941 cgagggagaa caatctcagg ctccggtcga gtcggtccag ctcgtcgatg tcccagagcc
     9001 cgtagtagac ccggtacttg aaatcgtgat gcaccggtct gagacgggtg tgtcggaccg
     9061 tgcccctgta aagggccgaa tgaaatccgc tcacaccagg gctccgatcc ggtccggggg
     9121 tagcgacgag ctcatcggtt cgaaggagcc ccaccatggc ggagggctgc ccaatgccgc
     9181 ggccacattg agccccgcct gaaggccgtc ctcatggaag ccgtagccgc aataggcgcc
     9241 cgcaaaccaa gttcgcctgg ccccttggat cttggccaac cttcgctgtg catccacagt
     9301 tcggtgatcg aattggggat gtgcatagtc gaaccgtcgg tgagtcagtg aaggatccgg
     9361 atcctggatc gggttgagcg acacaaagag ttgctttccg gtgtcaaggc gctgcagccg
     9421 attcatccag taggtgaccg aggcaactct cgacccgcga tctgcagggg agaccatggc
     9481 attccaactc gaccagaccc ggcgccgggt tggcatcaag gaggggtcac tgtggagcac
     9541 cgcatggttc ggctcgtatc gaatctctcc gagcacgttt cgctcggtgt ccgttgcgtt
     9601 ccgaccgagg attgccagcg agtgatcgga gtgagttgcc aacaccaatt ggtcgaaact
     9661 ctcccgccgg ccgttggctg ttatctcaac gcgatcctca tgacgctcga ccagggtgac
     9721 aggggttgca acccggaccc tgtccttgaa cgggtgggcg aggcgccgta cgtattcccg
     9781 gcttccgccg gacaccgtcc gccacagggg tctgtcggtg atgcggatca atccgtgatt
     9841 gttgaggaat ctgaggatcg agaccgcagg gaatttgcgg atctcgctct ggcgggctga
     9901 ccagatcgat gccgtcatgg gcaacaggta ctcctcgaga aaggcggcac cgaacccatg
     9961 gcgagtgagc aactcatcga tggtctcgtc ctgtctcggc ctcaactcgg agccgatcct
    10021 ccgaaaccgc atgatgtccg agatcatcga tcgataactt gcacggagaa ggttggttgg
    10081 ctgggcgacc aaacctctcg cgctggcggc gtactcgaag gatttgccca tggagaaggc
    10141 gaaggacatg tcgctcccct cggtgttgac tcccaaagcg gagaagagcc tggtgagatg
    10201 cgggtaggtc acttcgttgt agacgatgaa tcctgtgtcg accggcaccg ccccactcgg
    10261 ggtttcgacc tcgaccgtgt tggagtgacc accaagccgc tcgtccgcct catagaccac
    10321 gatttcgttg tcccggtgca aggcccaagc ggcgcccagc cctgagatgc ctgcaccgac
    10381 cacggcaatt ctctgtccca cgaaggtagt tcggagcggt cccgcggagc ggatgacaaa
    10441 atcgcgcggt gttccgccat ccgtccaggc gccccattcc gaatagttgc ctatgagact
    10501 tcaagagcag agaacggtct tgcgcgggct tgacgaagtg ttcgcctaca ccgccgactt
    10561 ctccaatatc gagaactggg accccggggt ggctcggtcg gccaaggtcg gcgtggaagc
    10621 ggtaggggtg ggcacccggt tcgatctcga cgtgaagttc ggatcgcgga tagtccccat
    10681 gacctacgag gtgaccgttt acgagccgcc caaacgtgtg gtgctcgttg gcaccggtga
    10741 caccgtcggc gcgatcgatg agattcggtt ttcgtcccag aatggccgca cgatcatcga
    10801 ctacaccgca gacatcgagt tccggaactt cattcgattc ctgaccccgt tcatgggctc
    10861 gacgatgcgg aaagtcgggg agagggcact cgacgggctg gtgaccgccc tcgacggatg
    10921 aacaagctgt cgatcgcagg gttcaccaac ctcgggtttc aacttcactc acgctccttc
    10981 gagcctgtag gcgcggactt gaccagcaag accgtgctga tcaccggcgc cagcggtggg
    11041 ttgggagagg cggccacggt ggctttgacc ggtctcgggg cccgagtctt ggcggtgggc
    11101 agggaccggg ccaagctcga tgcggttgaa caacgaaccg ccggtcg
//
LOCUS       DBNHXU010000423         2078 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3962257, whole genome shotgun sequence.
ACCESSION   DBNHXU010000423 DBNHXU010000000
VERSION     DBNHXU010000423.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3962257"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(49..837)
                     /locus_tag="AC161X_10240"
     CDS             complement(49..837)
                     /locus_tag="AC161X_10240"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007120512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HIR4989978.1"
                     /translation="MELRDKVIVITGAGSGIGAALARRFSSEQPHALVVADLNFAAAE
                     DVATDIGAVAIGVDVTRPEDNDRLIDEVEELFGPIDLFCANAGIGFAGDEQTPPDEWN
                     RMWQVNVMSHIHAARRLIPMWSARGSGYFLATVSAAGLLTNLKAAQYSVTKHAALAFS
                     EWLAITYGDQGIKVSALCPMGVKTPFLDSAAEFRDLLLPDAIEPEALAEAVVAGLEAE
                     RFLILPHPEVARFFQNKANDYDRWLEGMRRLQRSAFPTPRIGQN"
     gene            complement(858..>2078)
                     /locus_tag="AC161X_10245"
     CDS             complement(858..>2078)
                     /locus_tag="AC161X_10245"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012656.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HIR4989979.1"
                     /translation="YRLFGTKIFITWGDQDLTDNIVHFVLARLPDSAPGTKGISMFVV
                     PKFIPDDRGEPGPRNDYKIVSLEHKLGIHASPTCVISFGDAGEGAVGHILGEPNAGMR
                     NMFTMMNAARIGVGMEGVSIGERAYQRALQYARDRVQGRAVGTTGAGRVPIIEHPDVR
                     RMLLTMKAYVEATRCLLYVAAAEADHQLHGETEERRQAAGNRLALLTPIVKAWSTDIG
                     VEVASLGIQVHGGAGYIEETGAAQYLRDSRIAPIYEGTNGIQAIDLVLRKLPLENGVV
                     VTTLITEMTDVVIAMNSHEELAAFGDELSRAIRGLGESSAWLGERLAAGDIQSALAGA
                     SPYLRQFGTVVGGWLMGKAALAALDAPSDFTPEFLAEKVTTARFYGEQLLPTANGLIP
                     AIKGGADILENAVF"
BASE COUNT          348 a          659 c          675 g          396 t
ORIGIN      
        1 gcgacggctc cagtgccggt caccaccact cgccttctgc tcatgtgctc aattctgccc
       61 gattcgcgga gttggaaacg cagaacgttg cagtctgcgc attccttcca gccatcggtc
      121 gtagtcgttg gccttgttct ggaagaaccg ggccacctcg gggtggggga ggatcaggaa
      181 tcgctccgcc tccaaaccgg cgaccaccgc ttcggccagc gcctccggtt cgatggcatc
      241 gggcaggaga aggtcccgga attcggcggc cgaatcgaga aacggggtct tcactcccat
      301 ggggcagaga gcgctcacct tgatgccctg gtcgccgtag gtaatggcga gccattcgga
      361 aaaggccagc gcagcatgct tggtcaccga atactgcgcc gctttgaggt tggtgagtag
      421 gccggccgcc gaaaccgtgg caaggaaata accgctgccc cgagccgacc acattgggat
      481 caggcggcgg gcggcgtgga tgtgggacat gacgtttact tgccacatcc gattccactc
      541 gtcgggaggc gtctgctcgt ctccggcgaa tccgatcccg gcgttggcgc agaacaggtc
      601 gatcgggccg aaaagctcct cgacctcgtc gatcaggcgg tcgttgtctt cgggtcgggt
      661 cacgtcgacc ccgattgcaa ctgctccgat atcggtcgcc acatcctctg ccgccgcgaa
      721 gttgagatcg gcgacaacca gcgcatgtgg ctgctcgctg gagaaacggc gggccagcgc
      781 tgccccgatc ccgctaccgg cgccggtgat gacgatgacc ttgtcccgaa gctccatgtt
      841 cggaggctac gtgggtgtca gaacacggcg ttctccagga tgtcggctcc gcccttgatc
      901 gccgggatca ggccgttggc agtcggaagc aactgttcgc cgtagaaccg ggcggttgtg
      961 accttctcgg ccaggaattc cggcgtgaag tcggagggag cgtcgagcgc tgccagcgcc
     1021 gcctttccca tgagccaccc gccgacgacg gtgccgaact ggcggagata ggggctggcg
     1081 ccggccaggg cggactggat gtcgcccgca gccagtcgtt ctcccagcca ggccgatgac
     1141 tcgccaagcc ctctgattgc ccgggacaac tcgtctccga aagcggccag ctcctcgtgg
     1201 ctgttcatgg cgatcaccac atcggtcatc tcggtgatca gcgtggtgac caccacgccg
     1261 ttctcgaggg gcagtttgcg aagaacgagg tcgatggcct ggatcccgtt ggtgccctcg
     1321 tagatgggcg cgatcctcga gtcacgaagg tattgggcgg cgcccgtctc ctcgatgtag
     1381 cccgctcctc cgtgcacctg gatgccgagc gaggcgactt ccactccgat gtcggtcgac
     1441 cacgccttca cgatcggggt gaggagggcg agccggtttc ccgctgcttg acggcgctcc
     1501 tcggtctcac cgtggagctg gtgatcggcc tcagccgctg cgacgtagag aaggcaccgg
     1561 gtcgcttcga cgtatgcctt catcgtcagc agcatccgtc gaacgtcggg gtgctcgatg
     1621 atcgggaccc gacctgcccc ggttgttccc accgcgcgac cctggacccg gtctctcgcg
     1681 tattggaggg cccgttgata ggcccgctct ccgattgaca ctccctccat gcccaccccg
     1741 atgcgggctg cgttcatcat cgtgaacatg ttgcgcatgc cggcgttcgg ctcccccagg
     1801 atgtgcccga cggcgccctc cccggcgtca ccgaaggaga tcacgcaggt cggggaggcg
     1861 tggatgccga gcttgtgctc cagggagacg atcttgtaat cgtttcgcgg ccctggttct
     1921 cccctgtcgt cgggaatgaa cttgggcact acgaacatcg agatgccctt ggtgcccggg
     1981 gcagaatcgg ggagacgggc cagaacaaag tggacgatgt tgtcggtcag gtcctgatcg
     2041 ccccaggtga tgaagatctt ggtgccgaag agccggta
//
LOCUS       DBNHXU010000424         1676 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2175504, whole genome shotgun sequence.
ACCESSION   DBNHXU010000424 DBNHXU010000000
VERSION     DBNHXU010000424.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1676)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1676)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1676
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2175504"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1600)
                     /gene="nuoL"
                     /locus_tag="AC161X_10250"
     CDS             complement(<1..1600)
                     /gene="nuoL"
                     /locus_tag="AC161X_10250"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HIR4989980.1"
                     /translation="MSFAVLAAETEHVVEAGVTGGFWVDTAGIMLILPFVAFGLIMIF
                     GKRMKRQGAEIAIGALAINTVWAVVLFVMNMTEGVLAKTTFEIARIGTVAGHEIVFEL
                     GWVVDGLSIMMFFLVNVVGLLVFIYAVGYMHGDVRVPWFFAAFSLFAGSMLLLVGAPN
                     LIQLIIGWEGVGLASYLLIGFYWEDLDNVRAGNKAFLTNKVADVGLILGAIVVAIAVG
                     SFDFEAINVAAVDHLDALASVAVVGGLLLFLGAMGKSAQFPLHIWLPDAMAGPTPVSA
                     LMHAATMVTAGIYLMARTFPLFQNLAEEARPWMVAIGTVTLFAMGLLALVANDIKKVL
                     AYSTVSQLGYMMTAVAAGGYTAGLFHLFTHAFFKALLFLGAGSVIHAVHSNNMTDMGG
                     LRKQMPVTYWTFLIGSLSLAGIIPLAGFWSKDEILVTLGHEGYTAVMWIAIAGAFVTA
                     FYMTRAVALTFFGAYKGHGHPHESPRVMTVPLVLLAIPSAIAGLFSIPGVTFPGIGNF
                     SEWLAVREVSMGDHHAEGIDFLLAG"
BASE COUNT          382 a          513 c          516 g          265 t
ORIGIN      
        1 tgcccgccag gaggaagtcg atcccctctg cgtggtgatc ccccatcgaa acctcgcgca
       61 ccgccagcca ttcgctgaag ttgccgatcc cgggaaaagt gaccccaggg atgctgaaca
      121 gtccggcaat cgccgaggga atcgcgagca ggaccaatgg aaccgtcatc acacgtggag
      181 actcatgggg gtggccatgt cccttgtagg ccccgaagaa ggtgagggcg actgctctcg
      241 tcatatagaa ggcagtgaca aaggccccgg cgatggcgat ccacatcact gcggtgtaac
      301 cctcatgtcc cagcgtcacc aggatctcat ccttggacca gaagccggcc agcgggatga
      361 tccccgccag ggacaaggat ccaatgagga aggtccagta ggtgaccggc atttgtttgc
      421 gcaggccacc catgtcagtc atgttgttgc tgtggacggc gtggatgacc gagcccgccc
      481 cgaggaagag caacgccttg aagaaggcgt gggtgaacag gtggaacagc ccggcggtgt
      541 acccgcctgc ggccaccgca gtcatcatgt agccgagctg agacaccgtc gagtaggcga
      601 gcaccttctt gatgtcgttc gccaccaagg cgagcaggcc cattgcgaac agggtgacgg
      661 tgccaatcgc caccatccag ggccgagcct cctcggccag gttctggaag agtgggaacg
      721 tgcgagccat gagatagatg ccggcggtga ccatggtcgc ggcgtgcatc agcgccgaca
      781 ccggtgtggg gccagccatc gcatcgggca gccagatgtg gagcgggaac tgggcgctct
      841 tgcccatggc accgaggaat agaagtaggc ccccgaccac cgccaccgac gccagtgcat
      901 ccaggtggtc cacggccgca acgttgatcg cctcgaaatc gaagctgcca acagcgatgg
      961 caaccacgat ggcacccagg atcaggccga catcagccac cttgttggtg aggaaggcct
     1021 tgttgccggc tcgcacgttg tcgaggtcct cccagtagaa cccgatcaga aggtacgaag
     1081 cgaggccaac accttcccag ccgatgatga gctgaatcag gttgggagca cccaccaaca
     1141 gaagcatcga ccctgcgaat aaggagaacg cggcgaagaa ccaggggacc cgaacgtcgc
     1201 catgcatgta accgactgca tagatgaaga ccagcaggcc gaccacgttg accagaaaga
     1261 acatcatgat cgacagcccg tcgaccaccc agccgagctc aaagacgatc tcgtggccgg
     1321 cgactgtccc gatccgggcg atctcgaacg tcgtcttggc gagaacccct tcggtcatgt
     1381 tcattacgaa gaggacgacg gcccagacgg tgttgatggc gagggctcca attgcgatct
     1441 cggcgccctg ccgcttcatc ctctttccga agatcatgat cagaccgaag gcaacaaagg
     1501 ggaggatcag catgatccct gcggtgtcga cccagaaacc tccggtgaca cccgcctcga
     1561 cgacgtgctc ggtctcagcg gcgagaacgg cgaagctcat caccatctca tcaggctcag
     1621 gtcgtcgacg ttggccgagc ggcggttgcg gaagatcatg atcacgatgg cgagcc
//
LOCUS       DBNHXU010000425         4389 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_1780299, whole genome shotgun sequence.
ACCESSION   DBNHXU010000425 DBNHXU010000000
VERSION     DBNHXU010000425.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 4389)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 4389)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4389
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_1780299"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..278)
                     /locus_tag="AC161X_10255"
     CDS             complement(<1..278)
                     /locus_tag="AC161X_10255"
                     /inference="COORDINATES: protein motif:HMM:NF016213.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF456 family protein"
                     /protein_id="HIR4989981.1"
                     /translation="MSPLGEFVVALAILAGLFGIVLPFLPGLLLIVAAVVVWAFAVGT
                     PIGWAVAVVVLAIGVGGTIFKYLVPGRRLKEVGLPTSTLVIAIGVAIVG"
     gene            complement(306..1088)
                     /locus_tag="AC161X_10260"
     CDS             complement(306..1088)
                     /locus_tag="AC161X_10260"
                     /inference="COORDINATES: protein motif:HMM:NF012955.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HIR4989982.1"
                     /translation="MTAGPLFSRRMAIAEMGKAGLAIAVLGIAACSKEGSQSGSSIQG
                     GSSNPSTSVPSSATSSTVFAGSSYRRINLGFVNAYILFRGGEAAVVDTGISGSEEAIE
                     AALGEIGLGWDAVGHVIVTHKHPDHQGSLAAVVALAPAAQVYAGAEDIPEMETLATPT
                     AVGDGEQVFGLEIIKTPGHTPGHISVLDEAAGVLVTGDALNGGSGGVTGANPSFSEDM
                     TTANASVVKLAGFTYETALFGHGEPVLTMASAQVNELASSLG"
     gene            1156..2511
                     /locus_tag="AC161X_10265"
     CDS             1156..2511
                     /locus_tag="AC161X_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012852030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0000162 - L-tryptophan biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006568 - L-tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TrpB-like pyridoxal phosphate-dependent enzyme"
                     /protein_id="HIR4989983.1"
                     /translation="MDTKITLPESELPTHWYNIVPDLPVPPPPVLHPGTHEPVGPDDL
                     APLFPMDLILQEVSQDRFVEIPEEVREVYRMWRPTPLYRAKNLEKSIGGAARIYYKYE
                     GVSPAGSHKPNTAVAQAYYNAKAGVKKLSTETGAGQWGSALSFACALFGIELEVYMVR
                     ASYDQKPYRRAMIEAWGASVYPSPSDRTGAGRAILEADPGSTGSLGMAISEAVEVAAT
                     HDDTKYSLGSVLNHVLMHQTVIGEEALKQMALVNETPDVIIGCAGGGSNFSGLTFPFI
                     RDKLAGTTNPTVIAVEPAAAPSLTRGSYQFDFGDAVGMAPIVKMHTLGHDFVPDPIHA
                     GGLRYHGMAPLVSLLHDQGVIEAVAKHQSETFTAAVRFARTEGILPAPESSHAIAEAI
                     TQAERAKEAGEEKVILFNLSGHGHFDMGAYQSHFDGSMEDYEYPAERIAAAMEKVPVI
                     A"
     gene            complement(2622..3440)
                     /locus_tag="AC161X_10270"
     CDS             complement(2622..3440)
                     /locus_tag="AC161X_10270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989984.1"
                     /translation="MGRKTIGLLAAATLTLVACGTEVAPLDPPDDPVTPVLRIQYEGG
                     FVPLEWSLGSGPTYELLSDGRLIVPGVTTMQYPGPLVVPYQQVQLSEADVDRIMAVLE
                     DMGIAEMTDEKDDSQAAHIADAATYVITFWDDEGAHKYSVYALGIESDSNNAATQAAV
                     QLDALMLELSSGATEPFVAEEIKVLAGVSTAQLEPGFEDVRPWPLPDDPQEWGGFSGI
                     DWKCNVYPGDDIDLFTDATQATTWTPPSGEGDPYTLLVRQLLPGEEGCRELASP"
     gene            complement(3478..4014)
                     /locus_tag="AC161X_10275"
     CDS             complement(3478..4014)
                     /locus_tag="AC161X_10275"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015803391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0071554 - cell wall organization or
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HIR4989985.1"
                     /translation="MTEEDLSASWRPGCPLDVSELRAVDVSHWGFDGEVHTGRLIVAT
                     DLAEDVVEIMRDLFDASFPIERMEPVDAYDGNDDQSMAANNTSAFNCRPVTGGSSWSE
                     HSYGRAIDVNPLVNPYVVRSTVLPPEGAAYADRSQNAPGMIHDGDAVVDAFAARGWKW
                     GGYWDSPTDYQHFSTTGH"
     gene            4046..>4389
                     /locus_tag="AC161X_10280"
     CDS             4046..>4389
                     /locus_tag="AC161X_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009477352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989986.1"
                     /translation="MVVVEGADVVVVVVVVVVVVVVAIVIVVEDTRDGVVVVMDDEVA
                     VVLAAQPPIRSATSITPADRPSPCMNRVSQVGLGLLGRSKGDLLGAVIGEVLGEAVGV
                     AISPIPIIAVVL"
BASE COUNT          800 a         1378 c         1415 g          796 t
ORIGIN      
        1 cccacgatgg cgacgccgat cgcgatcacc agggtcgacg tgggaaggcc gacctccttg
       61 agtcggcgtc cggggaccag gtacttgaag atcgtgcccc cgactccaat cgccagcacg
      121 accacggcca ccgcccatcc aatcggagtt ccgacagcga aggcccagac gacaaccgcg
      181 gcaactatca ggagcagccc gggcaggaac gggaggacga tcccgaacag gccggcaaga
      241 atcgccaatg ccaccacgaa ctctccaagc gggctcatgt ccggatcgta aggatttgcc
      301 ggcgttcagc cgagactcga agcgagttcg ttgacctggg ccgaagccat ggtgagaacc
      361 ggctcaccat ggccgaagag ggcggtttcg taggtgaacc cggccagctt gaccacggag
      421 gcattggctg tcgtcatgtc ctcggagaat gacgggttgg ctccagtcac gcccccactc
      481 ccgccgttga gggcgtcacc ggtcacgagg acccccgctg cctcgtcgag caccgaaatg
      541 tgcccgggtg tgtgccccgg agtcttgatg atctccaggc caaagacttg ctccccgtcg
      601 cccaccgccg tcggcgtggc aagggtctcc atctcaggga tgtcttcggc accggcatag
      661 acctgcgccg cgggcgccag cgccaccact gcggcgagac tcccctgatg gtcggggtgc
      721 ttgtgggtga cgatcacgtg gccgaccgcg tcccatccca acccgatttc cccgagggcg
      781 gcctcgatcg cctcctcgct ccctgagatc ccggtgtcga cgacagcggc ctccccgccg
      841 cggaagagga tgtatgcgtt gacgaacccg agattgatcc ttctgtagga ggagccagcg
      901 aacacggtgc tcgacgtcgc cgacgatggg accgacgtcg atgggttgct cgagccgccc
      961 tggatgctgc tgcctgattg gcttccttcc ttggagcagg ctgcgatgcc gaggacggcg
     1021 atcgccagcc cggccttacc catctccgcg attgccatgc ggcggctgaa caaggggcca
     1081 gcggtcatcg agtcgctccc ggatagctct tcccgtaccc aacatacccc caaccccgcg
     1141 gtgtaacttc aaaccatgga caccaagatc accctgccgg agtcagagct gccgacgcac
     1201 tggtacaaca tcgtccccga tctcccggtg ccccctccac ccgtccttca tccgggcacc
     1261 cacgagccgg tggggcccga cgatctcgca cctctgttcc cgatggatct gatcctccag
     1321 gaggtgagtc aggaccgatt tgtcgagatt cctgaggaag tgcgggaggt ctatcggatg
     1381 tggcgtccga cgccgcttta ccgcgccaag aacctggaga agtcgatcgg cggcgccgcc
     1441 cggatctact acaagtacga aggcgtcagc ccggccggat cccacaagcc caataccgcg
     1501 gtggcccagg cctactacaa cgccaaggcc ggggtgaaga agctctccac tgagaccggt
     1561 gccggccagt ggggctcggc gctctcgttt gcctgcgcct tgttcggcat cgagctggag
     1621 gtctacatgg tccgggcctc ctacgaccag aagccctatc gccgggcgat gatcgaagct
     1681 tggggtgcca gcgtgtatcc gtcaccatcg gatcggaccg gtgccgggcg cgccatcctc
     1741 gaggctgacc ccggctcgac tgggagtctt ggcatggcga tttccgaggc ggttgaggtg
     1801 gccgccaccc acgacgacac caagtactcg ttggggtcgg tcctcaacca cgtgctgatg
     1861 catcagaccg tgatcggcga ggaagcgctg aagcagatgg cgctggtcaa cgaaacgcca
     1921 gatgtcatca tcggttgtgc tggtggcggc tccaacttct caggattgac cttccccttc
     1981 atcagggaca agctggccgg aaccaccaat ccgaccgtga tcgccgttga gccggctgcc
     2041 gccccgtcat tgaccagggg cagttaccag ttcgatttcg gcgacgcggt ggggatggcc
     2101 cccatcgtca agatgcacac cttgggccat gatttcgtgc ctgacccgat ccacgccggc
     2161 gggcttcgct atcacgggat ggcgcctctg gtgtccttgc tccacgatca aggggttatc
     2221 gaggccgtgg cgaagcatca aagcgagacc ttcaccgctg cagtcaggtt cgcccgcacc
     2281 gaaggcatcc tccccgcgcc ggagtcgagc cacgccatcg ccgaggcgat cactcaggcc
     2341 gagcgagcca aggaggcggg tgaggagaag gtgattctct tcaacctctc ggggcacggt
     2401 catttcgaca tgggcgccta ccagtcccac ttcgacggtt cgatggagga ctacgagtac
     2461 ccggcggagc ggatcgcggc cgcgatggag aaggttccgg tcatcgccta gctgtttctc
     2521 cccccagcga acgaagctgt ttcctccccc aggagcggag cgacgttggg ggcggtggcc
     2581 cggagggccg gagggggcta ctccaacgcg agacccgcaa gctagggcga ggcgagctcc
     2641 cgacagcctt cctcgccggg cagaagctga cggaccagca aggtgtaggg gtcgccttca
     2701 cctgacggcg gggtccatgt cgttgcctgg gtcgcatccg tgaacagatc gatgtcgtcg
     2761 cccgggtaga cgttgcattt ccagtcgatc ccggagaacc cgccccattc ctgaggatcg
     2821 tcgggcaacg gccaggggcg aacgtcttcg aagccaggtt cgagctgagc cgtgctcacc
     2881 ccggccaaga ccttgatctc ttcggccacg aagggctcgg ttgctcccga ggacaactcc
     2941 agcatcaagg cgtccaactg gaccgccgcc tgggtggcgg cattgttgga gtcggactcg
     3001 atgccgaggg cgtagaccga gtacttgtgc gctccttcgt cgtcccagaa ggtgatcaca
     3061 taggtggcgg cgtcggcgat atgagccgcc tgggagtcgt ccttttcgtc cgtcatttca
     3121 gcgattccca tgtcctcgag cacggccatg atccgatcga catcagcctc gctcaattgc
     3181 acctgctggt agggaacaac caaaggccct ggatactgca tggtggtgac accggggacg
     3241 atcaaacggc cgtcgctcaa aagctcatag gtgggaccgg agccgagact ccattccagg
     3301 gggacgaatc caccttcgta ttggatgcgg aggaccgggg tgaccgggtc gtcaggtgga
     3361 tcgagcgggg cgacttcggt cccacaggcg acaagagtca aggtggctgc ggcgagcagt
     3421 ccgatggttt tccttcccat gggtcttaga tgtcctcggt gcactcggcg gttcccgtca
     3481 atgtcccgtg gtcgaaaagt gttggtagtc ggtgggcgaa tcccagtatc cgccccactt
     3541 ccatccccgg gcggcgaagg catcgacgac cgcatccccg tcatggatca ttcccggggc
     3601 gttctggctc ctgtctgcgt aggccgcacc ttcgggtggg agaacggttg accggacgac
     3661 gtaggggttg accaacgggt tgacgtcgat ggctcgaccg taggagtgct ccgaccagga
     3721 tgagcccccg gtcaccggac ggcagttgaa ggcactggtg ttgttggcag ccatgctctg
     3781 gtcgtcattg ccgtcgtacg catcgaccgg ctccatgcgc tcgatgggaa acgaggcatc
     3841 gaacaggtcc cgcatgatct ccacaacgtc ctcggccaga tctgtggcca cgatcagacg
     3901 gccggtgtga acttcaccgt cgaatcccca atgggacaca tcgaccgcgc gaagttcgga
     3961 cacgtcgagt gggcatccag gacgccagga cgccgaaagg tcctcctcgg tcacgtgcga
     4021 caccctatga gcgaacccag gcgaggtggt tgtcgtcgaa ggtgccgacg tggtggtcgt
     4081 ggtggtcgtg gtggtcgtgg tggtcgtggt ggccatcgtg atcgtggttg aggacaccag
     4141 ggacggagtt gtggttgtga tggatgacga ggtcgccgtc gtattggcgg cgcagccgcc
     4201 gatcaggagt gccacgagca ttactcccgc tgaccggcca agtccttgca tgaatcgagt
     4261 gtcacaggtt ggcctaggtt tgctcggaag gagcaaagga gatctgttgg gtgctgtgat
     4321 cggtgaggtc ctgggcgagg cggtgggagt ggcgatcagc ccgataccga taatcgcggt
     4381 ggtactcat
//
LOCUS       DBNHXU010000426         1650 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_2971665, whole genome shotgun sequence.
ACCESSION   DBNHXU010000426 DBNHXU010000000
VERSION     DBNHXU010000426.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1650)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1650)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1650
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_2971665"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="AC161X_10285"
     CDS             complement(<1..704)
                     /locus_tag="AC161X_10285"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012371.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HIR4989987.1"
                     /translation="MKDLKAGGLGAWIDDQAGQQSFSGVALVWEAGEPVFTHAAGLAH
                     RGHQVPVTLDTRFQVASVTKMVTATAALRLVERGTLRLDRPLLDFLPPDLRPASLDKR
                     HTLHHFLSHTSGLTNYHDDEDETWASFTSAWDRVPVYHARGPKDILPLFADLPAASDP
                     GEFLYADANFILIGVLIEWVTGKSFAEVAAEEVLGPAGMDDSGFFQIDLEPRNMATGY
                     LATDEPPDTWRSNIYSV"
     gene            complement(701..1288)
                     /locus_tag="AC161X_10290"
     CDS             complement(701..1288)
                     /locus_tag="AC161X_10290"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HIR4989988.1"
                     /translation="MARPYFETDRLIIQRFTRRDQATLDDAIRSSLPELNQWLPWARM
                     DYEYGDTTAFIRESLQAWKEERAWDYTIRYKAKPERHLGNVSLWTVSKLGKIAEFGYW
                     IRTDENSKGICTEAMECLLGEAFGSLGYHKVTLRIAVGNDQSDRVASKLGFTREGVLR
                     EELLIRGNWVDHSLWSLLDREYRALRRSGEVHGTR"
     gene            1326..>1650
                     /locus_tag="AC161X_10295"
     CDS             1326..>1650
                     /locus_tag="AC161X_10295"
                     /EC_number="3.6.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012514.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016817 - hydrolase activity, acting on
                     acid anhydrides [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HIR4989989.1"
                     /translation="MWDRLHLSAEEPEPGDGPQAAVLAALYEDEDGLVRIVLTKRPDT
                     MPTHAGHISLPGGKADPSDRGPIDTALREAHEEVGIEPSSVEILGFLPSIDTVEFKPW
                     VVPVVG"
BASE COUNT          314 a          495 c          527 g          314 t
ORIGIN      
        1 acgctgtaga tattggaacg ccaggtgtct ggtggttcgt cggttgcgag atagccggtg
       61 gccatgttgc ggggctcgag atcgatctgg aagaagccgg agtcgtccat gcccgcagga
      121 cccagaactt cctcggcagc cacttcagcg aatgacttcc ccgtaaccca ctcgatcagc
      181 accccgatca ggatgaagtt ggcgtcggcg tagaggaact ctccggggtc tgaggcagcg
      241 gggaggtcgg cgaagagagg aagaatgtcc tttggccccc gggcgtgata gaccggtacc
      301 cggtcccagg cgctggtgaa agaagcccac gtctcgtctt catcatcgtg gtaattggtc
      361 aggccagagg tgtgggacag gaaatgatga agcgtgtgcc gtttgtcgag tgaggccggg
      421 cgtaaatcgg ggggtaggaa atcgaggagg ggacggtcga gtctcagtgt tcccctctcg
      481 accagccgca gagcggccgt tgcggtgacc atcttggtga cggatgcgac ctggaaacga
      541 gtgtcgaggg tgaccggcac ttgatgcccg cggtgggcga ggccggcagc atgggtgaag
      601 actggttcgc ctgcttccca caccaatgcc accccgctga acgactgctg gccggcctgg
      661 tcgtcgatcc aggcgcccag gccacccgcc ttcaggtcct tcatctggtg ccgtgcacct
      721 caccggaccg ccgaagggcc cggtactcac gatcgagaag cgaccacagc gaatggtcca
      781 cccaattgcc acggatcaac aactcctcgc ggaggacccc ttccctcgtg aaacccagtt
      841 tgctggcgac gcggtcggac tgatcgttgc cgaccgcgat tcggagcgtc accttgtggt
      901 agcccagcga tccaaacgcc tcaccgagga ggcattccat tgcctcggtg cagattccct
      961 tggagttttc gtcggtgcgg atccagtatc cgaactcggc gatcttgccc agcttggaga
     1021 ctgtccacaa cgagacgttc cccaggtggc gctcgggttt ggccttgtat cggatcgtgt
     1081 aatcccaggc cctctcctcc ttccaggctt ggagcgactc ccggatgaag gccgtggtgt
     1141 ctccgtactc gtagtccatg cgcgcccaag ggagccattg gttcaactcg ggcagtgagg
     1201 aacggatcgc gtcgtcgagg gtcgcctggt cgcgcctggt aaagcgctga ataatcagcc
     1261 gatcggtttc gaagtatggg cgcgccaccg gatcaggtta tcggcgcact taacctgacc
     1321 attgaatgtg ggacagactt cacctgagtg ccgaggaacc cgagcccggg gacgggcccc
     1381 aagccgcagt cctggccgct ctatatgagg acgaagacgg gttggtgcgg atcgtcctca
     1441 ccaaacggcc ggacacgatg cccacccacg ccggccacat ctcccttccc ggaggcaagg
     1501 ccgacccttc ggatcgaggg ccaatcgaca ctgcattgcg cgaagctcac gaagaagtgg
     1561 gcatcgagcc gtcctcagtg gagattctcg gcttcctgcc gtcgattgac accgtggaat
     1621 tcaagccatg ggtggttccc gtagtgggcc
//
LOCUS       DBNHXU010000427         1746 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_790653, whole genome shotgun sequence.
ACCESSION   DBNHXU010000427 DBNHXU010000000
VERSION     DBNHXU010000427.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1746)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1746
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_790653"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1013)
                     /locus_tag="AC161X_10300"
     CDS             complement(<1..1013)
                     /locus_tag="AC161X_10300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HIR4989990.1"
                     /translation="MRRALLFMTLAISVVACADQPEVVNPESLTLIAHDSFVEGVDAT
                     TFAGFTAGTGIDVNVIAGGDAGAMVNQLVLTKDNPLADVAFGVDDTFLSRAVDEDVFI
                     EHESPLLEDTVDGVHSTTPLVTSIDFGDVCINFDKQWFTDNGLTVPTTLDQLRDPTYA
                     SLLTVEHPATSSPGLAFVLATIDQYELHGWLDFWTDLRDGGVNVAPDWNTAYYGDFTR
                     YGGDSPMVVSYASSPPAEVVFAEEPIDEAPTGVIEDGCYRQIEYAGVLNGTLYPDASG
                     HLIDYMLSVEFQEKIPLTWFVFPANRDAELPEVFTENTTVPEAPVRFTYESFADREVW
                     IDE"
     regulatory      complement(1040..1152)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:15.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.5"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            1254..>1746
                     /locus_tag="AC161X_10305"
     CDS             1254..>1746
                     /locus_tag="AC161X_10305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015051931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HIR4989991.1"
                     /translation="MKVAMLAPISWRVPPRHYGPWEQFVSLLTEGLVRRDVDVTLFAT
                     ADSITSARLVGTVPTGYSEDPSLDPKVWEGLHIAAVFERADEFDLIHNSFDFLPLTYS
                     GLVDTPMVTTIHGFSSERIVPVYQKYNQHSHYIAISDSDRHPSLNYLATIHHGIDMSA
                     FEMS"
BASE COUNT          329 a          534 c          541 g          342 t
ORIGIN      
        1 cattcgtcga tccagacctc acggtcggcg aagctctcat aggtgaaccg gaccggtgcc
       61 tccgggacag tcgtgttttc ggtaaagacc tcgggaagtt ccgcgtctcg attggccggg
      121 aagacgaacc aggtgagcgg aatcttctcc tggaactcga cagacagcat gtagtcgatc
      181 agatgcccgg aggcatcggg gtacaaggtc ccgttgagaa ctccggcgta ttcgatctgg
      241 cgatagcacc cgtcctcgat caccccagtg ggagcctcgt cgatcggctc ctccgcaaac
      301 accacttcag ctgggggcga tgatgcatat gagacgacca tgggcgaatc cccgccgtag
      361 cgggtgaaat cgccgtagta ggcggtgttc caatcagggg ccacgttgac tccgccgtcg
      421 cggaggtccg tccagaagtc gagccaaccg tgcaactcat actggtcaat ggtggccagc
      481 acgaaagcca aacccggcga agaggttgcc gggtgctcga ccgtgagcaa tgaggcatag
      541 gtgggatcgc gcagttggtc caaggtggtg ggcactgtga gcccgttgtc ggtgaaccat
      601 tgcttgtcaa agttgatgca gacgtcccca aagtcgatgc tggtcaccaa tggggtggtg
      661 gagtggacgc cgtcgacggt gtcctccagc aaagggctct catgttctat gaacacgtct
      721 tcgtcgacgg cccgggagag gaaggtgtca tcgaccccga aggcgacgtc tgccagcggg
      781 ttgtctttgg tgaggaccag ttgattcacc atcgccccgg cgtcgccgcc ggcaatgacg
      841 ttgacatcga ttccggtgcc ggcagtgaag ccggcgaacg tggtggcatc cactccttcc
      901 acgaacgagt cgtgggcgat gagggtgagc gactcggggt tcaccacttc tggttggtcg
      961 gcacacgcga cgacagatat cgccagggtc atgaagagca gtgcccttcg catctcaatc
     1021 cactccttct acttgggagc gatgagtgcg ggtaaatccc tacgccggca ttatccgggt
     1081 caggttcttc cggtcggggt ctctgcagac cccctctcag cccggtctca ccgagctccc
     1141 gttgcgctgt caatgatagg ccgcgtggtt gtccccctgc gaagcggggg gaccgtccga
     1201 ccgaaggtcg ggcgaggggg gcaagcccga cgtgtcggta cgctctggct gagatgaagg
     1261 ttgccatgct ggccccgatc tcgtggcggg ttcccccccg ccactatggg ccgtgggagc
     1321 agttcgtttc cctgttgacc gagggcttgg tgcgccgcga tgtggatgtc accctcttcg
     1381 ccactgccga ttcgatcacc agcgcccgct tggtcggcac ggttccgacg ggctactccg
     1441 aggaccccag cctcgacccg aaggtgtggg aagggcttca catcgccgcg gtcttcgaac
     1501 gagccgacga gttcgacctg atccacaaca gctttgactt tctgcccctg acctacagcg
     1561 gcctggtcga caccccgatg gtcaccacca tccacggctt ctcctccgag cggatcgtgc
     1621 cggtctatca gaagtacaac cagcactccc actacatcgc gatcagtgat tcggaccgtc
     1681 acccgagcct gaactacctg gcaacgattc accacgggat cgacatgagc gccttcgaga
     1741 tgagcc
//
LOCUS       DBNHXU010000428         1662 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_3567462, whole genome shotgun sequence.
ACCESSION   DBNHXU010000428 DBNHXU010000000
VERSION     DBNHXU010000428.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 1662)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1662)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1662
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_3567462"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..814
                     /gene="folP"
                     /locus_tag="AC161X_10310"
     CDS             8..814
                     /gene="folP"
                     /locus_tag="AC161X_10310"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006980355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HIR4989992.1"
                     /translation="MMGVLNVTPDSFSDGGMHATASDAVAAGIAMSDAGARIVDVGGE
                     STRPGALSVTVEEELERVIPVVEELAGRGLLISIDTSKPEVASAAIEAGAVILNDVTA
                     CSAPSMAELVADTGVGVVLMHMKGSPRDMQEDPQYEDVVTEVAGFLAERASSLVGLGV
                     NREAIAVDPGIGFGKTVEHNLQLLAGLPDLAALGYPLVLGASRKSFLGKITSIENPSD
                     RDGVTAVTTALGYERGARIFRVHDVSSSRAALVTAATIVAPELWEEWSPD"
     gene            790..1284
                     /locus_tag="AC161X_10315"
     CDS             790..1284
                     /locus_tag="AC161X_10315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HIR4989993.1"
                     /translation="MGRMVAGLKPRGFTWVITDRLAVSERVGGSGFQHRRVRREEEIT
                     WLVEEAGINSVVSMLPGNQNLLAYKEAGMATYSVPVAPTFEAAEIEAFFATLSKAMTR
                     NRARVLVHRETIDDTVGGLLGGYLVVSGLVKDPILALAVIQQILGRALGPEGRSLIPA
                     TADS"
     gene            1281..1640
                     /gene="folB"
                     /locus_tag="AC161X_10320"
     CDS             1281..1640
                     /gene="folB"
                     /locus_tag="AC161X_10320"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012854811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HIR4989994.1"
                     /translation="MTDRIDLTGIEVWARHGALPEEKTKDQLFVVDVGIHFDTSRAAA
                     SDDLEHTIDYGSLAQAVHDLVTAESHNLIETVADRVATRVLEDARVQRVIVTVHKPQA
                     PIAVPFGDVSVTLDRSR"
BASE COUNT          303 a          503 c          529 g          327 t
ORIGIN      
        1 tggcgtcctg atgggagttc tcaatgtcac ccccgactcc ttttccgatg gagggatgca
       61 cgccacggcc tccgatgcgg tcgcagcagg gatcgcgatg agcgatgccg gtgcacggat
      121 cgtcgacgtc gggggagagt cgacccggcc cggagcccta tcggtgactg ttgaggagga
      181 attggaaagg gtcatcccgg ttgtggagga gctcgccggc cgaggtctgc tgatttcgat
      241 cgacacttcg aagccggagg tggcaagtgc cgccatcgag gccggggccg tgatactcaa
      301 cgatgtcact gcctgttccg ctccgagcat ggcggagttg gtggccgaca cgggggttgg
      361 ggtggtcctg atgcatatga agggttctcc gagggacatg caggaggatc ctcaatacga
      421 ggacgtcgtc accgaggtgg ctggctttct cgccgaacga gcctcatcgc tcgttggcct
      481 tggcgtcaac cgggaagcga ttgccgtcga tcccgggatc gggttcggca agaccgtcga
      541 gcacaacctc caattgcttg ccgggctgcc cgatctcgcc gcccttggct acccgttggt
      601 gctgggagcc tcgaggaagt cctttctcgg caagatcacc agcatcgaga acccgtccga
      661 cagggatggc gtgactgccg tcacgacggc cctcggatac gagcgaggag caaggatttt
      721 tcgagtccac gatgtttctt cgtcaagggc agcgctggtg acagccgcca ctatcgtggc
      781 cccagagcta tgggaagaat ggtcgccgga ctgaaaccac gtggattcac gtgggtcatc
      841 accgatcgtc ttgccgtgtc ggaacgggtg ggaggctccg gtttccagca ccgacgcgtc
      901 aggcgggagg aagagatcac gtggctggtc gaagaggcag gtatcaactc tgtcgtttca
      961 atgcttcccg gaaatcaaaa cctgctcgcc tacaaggaag cggggatggc cacctactcg
     1021 gtccctgtgg ccccgacttt cgaggcagcc gagatcgaag ccttcttcgc caccctctcc
     1081 aaggcgatga cccgcaatcg cgcccgcgtc ctggtccatc gcgagacaat cgatgacacc
     1141 gtcggcggcc tgctcggtgg gtatctcgtg gtctcgggcc tcgtcaagga cccaatcctg
     1201 gctttggcgg tgatccaaca gatcttggga agggctctcg gccctgaagg ccgttcgctc
     1261 atccccgcaa ccgccgattc gtgaccgatc gcatcgatct caccgggatc gaagtttggg
     1321 cccgccacgg cgcccttccc gaggagaaga ccaaagacca gttgttcgtt gttgatgtcg
     1381 gtatccactt cgatacttcg cgagccgcgg cctccgacga tctcgaacac acgatcgact
     1441 acggaagcct cgcccaagcc gtccatgacc ttgtgaccgc cgagagtcac aacttgatcg
     1501 agaccgtcgc cgatcgggtg gcgacccggg ttctcgaaga tgctcgggtt cagcgtgtga
     1561 tcgtcaccgt ccacaagcca caggcgccga tcgcggttcc gttcggtgat gtgtcggtca
     1621 cgctcgatcg cagtcgatga cgaggtacgc cgtcgctctc gg
//
LOCUS       DBNHXU010000429         2893 bp    DNA     linear   ENV 30-APR-2026
DEFINITION  MAG TPA_asm: Acidimicrobiia bacterium isolate MAG04727
            SRR25158249_k127_4365286, whole genome shotgun sequence.
ACCESSION   DBNHXU010000429 DBNHXU010000000
VERSION     DBNHXU010000429.1
DBLINK      BioProject: PRJNA1430164
            BioSample: SAMN56414134
            Sequence Read Archive: SRR25158249
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Acidimicrobiia bacterium (soil metagenome)
  ORGANISM  Acidimicrobiia bacterium
            Bacteria; Bacillati; Actinomycetota; Acidimicrobiia.
REFERENCE   1  (bases 1 to 2893)
  AUTHORS   Wei,Y.
  TITLE     An integrated global resource of wetland microbiomes linking
            environmental metadata, community profiles, and genome-resolved
            metabolic traits
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 2893)
  AUTHORS   Wei,Y.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-MAR-2026) College of Life Sciences, Zhejiang
            University, Xihu District, Hangzhou City 310000, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: MetaBAT2 v. v2.12.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: 10x
            Sequencing Technology  :: Illumina NovaSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 04/25/2026 07:41:50
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.11
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,066
            CDSs (total)                      :: 2,045
            Genes (coding)                    :: 2,038
            CDSs (with protein)               :: 2,038
            Genes (RNA)                       :: 21
            rRNAs                             :: 1, 1 (16S, 23S)
            complete rRNAs                    :: 1 (16S)
            partial rRNAs                     :: 1 (23S)
            tRNAs                             :: 18
            ncRNAs                            :: 1
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 6 of 7
            Pseudo Genes (internal stop)      :: 0 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2893
                     /organism="Acidimicrobiia bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SRR25158249_k127_4365286"
                     /isolate="MAG04727"
                     /isolation_source="soil"
                     /db_xref="taxon:2080302"
                     /environmental_sample
                     /geo_loc_name="Germany"
                     /lat_lon="52.47 N 13.26 E"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="AC161X_10325"
     CDS             complement(<1..758)
                     /locus_tag="AC161X_10325"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HIR4989995.1"
                     /translation="MFELSVGEILAYLLPVVGIACVIWALAITRHGRQREREVVRLSE
                     EAHRIKNDFVAMVSHELRTPLTSIAGFADTLVESWKELPKDEVDEFLSIINRQAIYLG
                     DLVEDVLVIPRLEADRLRFQAELFDLGDLIEDVAKMVFPNGGKKTSLVSLPDGVRVKA
                     DRRRVQQVIRNLMENARKYGGDQIMVEGFVMGDQYLIIISDNGPGVPDEETQKVFENF
                     EQLSKGDARESTGIGLGLPIARRLARAMGGEVWY"
     gene            complement(806..2800)
                     /locus_tag="AC161X_10330"
     CDS             complement(806..2800)
                     /locus_tag="AC161X_10330"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF014567.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hybrid sensor histidine kinase/response
                     regulator"
                     /protein_id="HIR4989996.1"
                     /translation="MDLNTPELTRIFREELDERSSRLVAGARALANDTLDPAWVPDLV
                     RDAHTIKGSAGLLGYDSIREAATRLEQLWRLVSDGHEPDADIVMAMENSAGVLVASMS
                     EGDGQLSDLTERLVGGIEASKHEDEAAPLRRPEPGSLGGLLSSVSESLLGGATRVDTG
                     DLYRLINRIVEVALDTEALADLALVSIEGSDPSVFRRSWRNQLEKLSTSIGDIQDQAV
                     ALANVSFRDAASTFPQFVRYLGRRMGKDVRFEILGDDVQLDRQIVDLLREPLRHLLVN
                     AIDHGIEGGAERIAAGKTATGVVRIAAQEVDEGVEVSVSDDGAGIDWDKVAAVAVQND
                     LPFDLTPLLFQPGFTTNEDVSDFSGTGEGLAAARDSVERVHGAVRVESVKGQGTTVTM
                     TLPMSMVLQNVVVVAAGDQFWGFPEASVEAAIPLSQAEIKTTKGGRELHFESAAIPCV
                     SLSQAVGTGGDADESEILIMNTRFGLVAVTVGELIDRRRVAVKNLGPILEGPGHVTGA
                     ALLGGGQVLVVIDPNFLGILARRRPRLAGRRPRILVVDDSAGVRQLLSATLNGAGFEV
                     ALAGGAREAVIAMAEGDFDAMVIDYSMPRSNGVELVRAMRAAEVRVPIVMVSGVATQE
                     EKRAAWEVGVNAYLDKFDLRQGVLTTTLRRLIGLEDVGTA"
BASE COUNT          550 a          962 c          865 g          516 t
ORIGIN      
        1 tcataccaga cctcgccacc catggcccgg gcgagccgac gggcgatggg caggccaagc
       61 ccgatccccg tcgattccct ggcatcgccc ttggacaact gctcgaagtt ctcgaacacc
      121 ttctgggtct cctcgtcggg gacaccaggg ccgttgtccg agatgatgat caggtactga
      181 tcacccatga cgaagccctc gaccatgatc tggtcgccgc cgtactttcg ggcgttctcc
      241 atcaggtttc ggatgacctg ctgcactcga cggcggtcgg ctttgaccct gacaccatcg
      301 gggagggaga ccagcgatgt cttcttccca ccattgggga acaccatttt ggccacgtcc
      361 tcgatcaggt cgccaaggtc gaagagctcg gcctggaaac gaagacgatc agcttcgagc
      421 ctagggatga cgagaacgtc ctctacgaga tcacccaggt agatggcctg gcgattgatg
      481 atcgagagga attcatcgac ctcgtccttg ggcagttcct tccaggactc gaccagcgtg
      541 tcggcgaatc cggctatgga agtcagggga gttcgcagct cgtggctcac catggcgacg
      601 aagtcgttct tgatgcggtg ggcctcttcg gacaagcgca ccacctcgcg ctctcgctgc
      661 cggccgtgtc tggtgatcgc cagcgcccag atgacacagg cgatacccac caccggaagg
      721 agataagcca gtatctcgcc gacagagagc tcgaacatcc tcaactttta tcggggagtt
      781 tcggggtcgg ctgaagttct acgactcaag ccgttcctac atcttcgaga ccgatgagtc
      841 ggcgcaacgt ggtggtgagg acgccctggc gcagatcgaa cttgtcgaga taggcgttga
      901 cccccacttc ccaggccgca cgcttctcct cctgggtggc cacaccggag accatgacga
      961 tcggcacccg aacctcggcc gcacgcatcg cacgcaccaa ctcgacgcca ttcgagcgcg
     1021 gcatcgaata gtcgatcacc attgcgtcga aatccccctc cgccatggcg atgaccgcct
     1081 cgcgggcgcc cccggcgagg gccacctcga acccagcgcc gttgagagtc gccgacagca
     1141 gttgtcgcac tccagccgag tcgtcgacca caaggattct ggggcgtcgc ccggccaggc
     1201 gaggccggcg tcgggccagg atgccgagga aattggggtc gatgacgacc agaacctggc
     1261 caccgcccag caacgctgcg cctgtgacat gccccggccc ttcgagaatc ggacccaggt
     1321 tcttgactgc cacccggcga cgatcgatca gctcgccaac ggtcacggca accaggccga
     1381 acctggtgtt catgatcagg atctccgact cgtccgcatc cccaccggtt cccaccgctt
     1441 gagagagcga gacacagggg atggccgcac tttcgaaatg aagctcacgc cctcccttgg
     1501 tggtcttgat ctcggcctgc gagagaggaa ttgcggcttc aaccgaggct tccggaaagc
     1561 cccagaactg gtccccggcc gcaaccacaa ccacgttctg aaggaccatc gacatcggga
     1621 gcgtcatcgt cacggtggtg ccctggccct tgaccgactc gacccggacg gcgccgtgaa
     1681 cccgctccac ggaatcccgg gccgcggcga gcccctcacc tgtgccggag aagtcgctga
     1741 cgtcttcgtt ggtagtgaac ccgggttgga acagcagcgg tgtcagatcg aagggaaggt
     1801 cgttttgcac cgccaccgcg gccaccttgt cccaatcgat cccggcgccg tcgtcggaca
     1861 ccgaaacctc gactccctca tcgacctcct gggccgcgat gcggacgacg ccggtcgccg
     1921 tcttccctgc tgcaattcgc tcggcgccac cctcgatccc atgatcaatc gcgttgacca
     1981 gcagatgacg cagcggctca cgcaacaagt cgacgatctg gcggtcgagc tggacgtcat
     2041 cgccaagtat ctcaaatcgg acgtccttgc ccatgcggcg gcccaggtac cgcacgaact
     2101 ggggaaatgt tgatgccgcg tctcgaaagg agacattggc caacgcgact gcctggtcct
     2161 gaatgtcccc aatcgatgtg ctcagcttct ccagctggtt ccgccaggat cgccggaaca
     2221 cagacggatc cgagccctcg atggacacca gggccaaatc ggccagggcc tcggtgtcca
     2281 gcgccacctc aacgattcgg ttgatcagac ggtagagatc gccggtgtcg acccgggtcg
     2341 caccaccgag caaagactcg gacacggacg acaggagacc tcccaggctt ccaggctcgg
     2401 gccgtcggag aggggccgcc tcatcttcgt gcttcgaggc ctcgattcca ccgaccagcc
     2461 gctcggtcag gtcggagagt tggccgtccc cttcggacat cgatgccacc agcacaccgg
     2521 ccgagttctc cattgccatg acgatgtcgg cgtcgggctc atggccgtcc gagaccaggc
     2581 gccaaagctg ctcgagtcgc gttgcggcct cacgaatgga gtcgtatccg aggagcccgg
     2641 ccgagccttt gatggtgtgg gcgtcgcgaa ccagatcagg gacccaggcc gggtccaggg
     2701 tgtcgttggc cagggcgcgg gcgcccgcga cgaggcgact ggacctctca tccagctctt
     2761 cacggaagat gcgcgtcagt tcaggggtgt tgaggtccac ctgcattttt cttctggccg
     2821 ataaggcgtt atcggcaagg cgatggggcc cctgaatggg cccagccagg ctttttcact
     2881 cccactcgat ggt
//
